LOCUS       DAOSHP010000001        64617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_355_length_64617_cov_8.977835, whole genome shotgun sequence.
ACCESSION   DAOSHP010000001 DAOSHP010000000
VERSION     DAOSHP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 64617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64617
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_355_length_64617_cov_8.977835"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLB33_00005"
     CDS             <1..430
                     /locus_tag="PLB33_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61930.1"
                     /translation="FSRSVINAPFFSFIVAMILYKVRKPGAVSLFGLGYGAMMARMSI
                     FGTLSIAAGGIVADIIISLILRDYSSNTKIAIFAPIYSVSGVISTFIMTTFFIKSSLY
                     SFGGTVAMAIGAVSVYAAGAVGAFFAMKLYQTRLSKCLTN"
     gene            598..1845
                     /locus_tag="PLB33_00010"
     CDS             598..1845
                     /locus_tag="PLB33_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOG61931.1"
                     /translation="MEDNLFEKLRILSDAAKYDVSCVSSGVERKNNTVGGIGNASAAG
                     ICHAWSSDGRCISLLKILLTNDCIYDCKYCLNRVTNPIKRATFTPEEVAELTIQFYRR
                     NYIEGLFLSSAVEKSPDHTMENIYRVLELLRYKYNFWGYVHVKVIPGANPLLVQKTGM
                     LADRMSVNIELPTRESLSLLAPQKKMENILKPMSLIHNEISRAIEDKSHFKNAQKFVP
                     GGQSTQMIIGATPDSDKTILSASQNLYDKFRMKRVFFSAYVPVISSPNLPAVSTMPPL
                     LREHRLYQADWLMRFYYYKSEELFTEVEQNLDLEFDPKMMYALRNIDKFPIEINNASY
                     EELLRIPGLGVTSAQRIIRERKNAQISYDSLKKMRTVIKRAKYFITVKGKYYGNTKID
                     PEAIKAEIRSKEIQRQMSIFELL"
     gene            1848..2576
                     /locus_tag="PLB33_00015"
     CDS             1848..2576
                     /locus_tag="PLB33_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOG61932.1"
                     /translation="MDYLYDGTFEGLMTCIYYHYKKEKATGIYEISEYQQSIVYEAEI
                     VKTDISKAKIVCDSINRQISKEAYIHVYYCYLSNDSKKENLILDFLIFAFKYGRDTMN
                     FYTHEKVLPINEIYKKVAREEHRVLGILRFSDIGGILFAKYSPDNDISILLADHFADR
                     YKHEKFIIYDEKRKKAVVYANNSWEIKENINIDNVEFSLDETKIHKLWKQYFTDLAIK
                     ERKNINLQFQFVPARYRKNMTEFK"
     gene            complement(2741..3427)
                     /locus_tag="PLB33_00020"
     CDS             complement(2741..3427)
                     /locus_tag="PLB33_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG61933.1"
                     /translation="MKKKILSLTLALIIALSSTSAFAAEQVYYTNFNIYDLCKNLGYY
                     YFEPVNTNNNIEIKTNIEKAPVNNGAETTQAPVNNGAETAQAPSNERATTEAATTAAP
                     SVSNYERRVVELVNVERQKNGLAPLTLDPAISNVARIKSKDMSDNNYFAHQSPTYGSA
                     GDMLSQHGIRYSAWGENIAAGQRTPEEVVSAWMNSPGHRANILGSHYTKIGVGYVNNG
                     RPYWTQMFTN"
     gene            complement(3747..4400)
                     /locus_tag="PLB33_00025"
     CDS             complement(3747..4400)
                     /locus_tag="PLB33_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOG61934.1"
                     /translation="MSSLVLKITALITMIIDHYGAIFQGNIIIYRIIGRLAFPIYCFL
                     LVEGYTHTSNIKKYASRLIAFAIISEIPFDLAFFGKIGFEHQNIFFTLFIGLAAIYFI
                     DNKEGKYSTNSAFIIIAASVIATLLRTDYQFIGIIYILAFYFTRNQAKHKKFLTIGLI
                     MFAVNLSAGMLQQFSLLALIPIYFYNKKEGRKNKILQISFYTAYPLHLIIFYLINTL"
     gene            complement(4563..4874)
                     /gene="trxA"
                     /locus_tag="PLB33_00030"
     CDS             complement(4563..4874)
                     /gene="trxA"
                     /locus_tag="PLB33_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG61935.1"
                     /translation="MNTVKITSDNFEQEVLKSDKPVLLDFWAAWCGPCKMISPIIEEI
                     AKEVTDAKIGKVNIDEQPELASAFGIMSIPTLVKIKDGKVVNKMIGVRPKASIVQMLA
                     S"
     gene            5112..5411
                     /locus_tag="PLB33_00035"
     CDS             5112..5411
                     /locus_tag="PLB33_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61936.1"
                     /translation="MEKLFLQTENGLIPLDEKMIEKYNLVKGRKSPFTRYYVVDETGN
                     HNLGSTKETSSDDVDRMPAGEGAEDDEIVEFSDTGTILSQSEIIDFSHGTDSEEP"
     assembly_gap    5469..5568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5655..6026
                     /locus_tag="PLB33_00040"
     CDS             5655..6026
                     /locus_tag="PLB33_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG61937.1"
                     /translation="MSSNEGFVCDCEVIHGDIVNEVKKRMPDDEDIYDLSDFFKVLGD
                     STRMRIIWALDESEMCVCDIAVLLNMTKSAISHQLRALRNANLVKFRKEGKTVYYSLK
                     DKHIRDIYEIGMEHIKEKYNK"
     gene            6076..6297
                     /locus_tag="PLB33_00045"
     CDS             6076..6297
                     /locus_tag="PLB33_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOG61938.1"
                     /translation="MTKTYKLQGLDCANCLAKMERKISKLKGVKSVTSNFFTEKMIIE
                     ADDGLMPEIEEAALKIIKKYEPQVVFEEA"
     gene            6309..8144
                     /locus_tag="PLB33_00050"
     CDS             6309..8144
                     /locus_tag="PLB33_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOG61939.1"
                     /translation="MKKLLWRLIISAIFFVSAIIINDSQLKLIMYIISYILAGGDVVK
                     RAIENIKNGQVFDENFLMSVASIGAFFIGEAPEGVGVMLFYQIGEMLQSYAVGQSRRS
                     ISSLMDIRPDYANVLRNGEVLTIPPEEVEIGEIIVIKAGERVPLDGIVVEGNSMLDTS
                     ALTGESLPREVITGSELLSGCINMNGLLNAKVTKSYSESTVNKILELVENASSRKSKS
                     EKFITRFARVYTPVVVVIAVVLAIIPPLIIKGATFNEWIYRSLAFLVVSCPCALVISV
                     PLSFFGGIGGASRNGILVKGSNYLESLAKTEIIVFDKTGTLTKGVFKVQKIHSEKIKP
                     EELLKLAAYAESYSNHPISLSLKEAYKKDIDIEKISQVEEISGHGVSAAVENIKVLAG
                     NDKLMEKFKIPYNKETSEGTLVHIAADGEYAGYILIADKIKDDAFEAVSDLRKHNIKD
                     IVMLTGDSESVAINTARELGIDRVYAGLLPADKVEKVEELISQKSKKVTLMFVGDGIN
                     DAPVLARADVGVAMGALGSDAAIEAADVVIMTDEPSKIAKAIEIAKNTIKIAYQNIIF
                     AISVKVIILVLSTLGLASLWAAVFADVGVTFIAVLNAMRALRLNN"
     gene            8580..9581
                     /locus_tag="PLB33_00055"
     CDS             8580..9581
                     /locus_tag="PLB33_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOG61940.1"
                     /translation="MGGSILKITNEQLKEMYLTMLKIRKFEKSASELFAAGKIPGFVH
                     LYIGEEAVATGACANLREDDYITSTHRGHGHIIAKGGELKYMMAELFGREDGYCKGKG
                     GSMHIADATKGILGANGIVGAGHNIAVGSGLAIQHKKTDQVCVCFFGDASTNQSTFHE
                     ALNLASIWKLPVIFVCENNGYGISVSQKRHQAIADVSVRASAYNMPGITVDGNDLFAV
                     YEAVSEAVARARKGQGPTLIECKTYRWRGHFEGDPVVYRPEGELEAWIKKDPIPKVEK
                     YLLDNGVMNEEEMKKMNDDVDKMVEEAIEFAEKSPFPSLESALKDVYSDIVEEGRVR"
     gene            9578..10552
                     /locus_tag="PLB33_00060"
     CDS             9578..10552
                     /locus_tag="PLB33_00060"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOG61941.1"
                     /translation="MKKMTYAEGIREAMRFKMREDPNVILFGEDVGKFGGCFGVSAGL
                     WEEFGDDRIRDTPISEGVIVGAAVGSAACGIKPIAEIMFIDFITVGMDQLVNQAAKMR
                     YMFGGEINLPMVIRVPAGAGIGAAAQHSQSLEGWLTHVPGLKVVYPSNARDAYGLLLS
                     SIDDQNPVVFIENKVLYAMQNDADENELKPIPLGKARIAKEGTDVTIITYGRQVYDAL
                     EAAEILAKDGINAEVIDLRTLYPLDKETVFNSVEKTHKVVLVSEECKRGAYTGEIAAM
                     IAEERFYECDAPIMRVGALNTPIPFATDLESYVLPKARDIELAVKSLF"
     gene            10612..11616
                     /locus_tag="PLB33_00065"
     CDS             10612..11616
                     /locus_tag="PLB33_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG61942.1"
                     /translation="MASIGIIANPASGKDIRRLVSHATVIDNNEKINIVERIVLGAQA
                     LGVEKVFVMPDSYNMGYRVEDKLNSCNELRCEINVVNMMRFDGMEDTVKAADYMEKND
                     DIKCVIILGGDGTNRAAAKSLKDTPIITISTGTNNVYPTMLEGTVAGMAAAVVASGRF
                     DDIRYVTKDKRIEIYKDGDLIDIALIDAVISKDVFIGSKAIWNIDTIEKIIATRSHPA
                     SIGFSSLVGCKKIIYPEDDFGAYVDINSGSVRIKAPVAAGVVESVSVSEPVILRLDDE
                     YEFTAKDRGTIALDGEREIEFKKDQKLIFKITREGPYHVDVIKALETAQENNFFIIKN
                     "
     gene            11632..12930
                     /locus_tag="PLB33_00070"
     CDS             11632..12930
                     /locus_tag="PLB33_00070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOG61943.1"
                     /translation="MAEFIVMPKMGLTMTKGMLTNWKKKEGDLVNKGDILFEVETDKI
                     TNKVEAKISGILRKILVDKETVDVFVPVGIIGRADEDISELLEQASKELKTDKPQKEE
                     GPKETRELSPAVQAGDRVKISPRAKKIAKDLKVDYNLVQGTGPGGAITEEDIRKFAEG
                     TKAKVSPTAAIVAEQLGVDIFNIEKDTRIMKADVIKYKLDESLAKYADPQEHRQSMTN
                     MRKIIAKRMLHSVQTSPSVSFNIKVDTTGMKELRDELKDTFKVSYTDILVKIVSEVLL
                     EFPLLNSTLDGDEIITRNYVNMGVAVALPAGLLVPVVKFTNVKGLKEISNEIKDLSER
                     AKTNDLLPDELTGGTFTISNLGMYGIESFTPIINQPEVAILGVNAIHKEPVVVNDEVV
                     IKPMMNLSLTADHRVVDGAVAAEFLAKLKKYIENPCLLLL"
     gene            13040..13945
                     /locus_tag="PLB33_00075"
     CDS             13040..13945
                     /locus_tag="PLB33_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOG61944.1"
                     /translation="MELKNYQDLIDRIRKTDSKKRVAVAAAHDEHTLEAVFRAADDKL
                     VEPVLIGNKAKIIEILKELNVEYDLNSIISTDSDKESAEKTVELINSNMADFIMKGKL
                     QTADLLRAVVDKEKGLRTGRVMSHVAFLEIPAYHKLIAVTDGGMMMYPNAEEKKQILE
                     NAVDVFRAMGYECPKVAVLAAVETVNPKMPETVDADILKKMNQSGEIKDCLVEGPISV
                     DLTFNRESAEIKGFKSPVTGEADILLVPSITTGNIMCKALIEMAGATMAGMIVGAKVP
                     VVLASRGASSQEKYLSIVLSASSVK"
     gene            complement(14076..14504)
                     /locus_tag="PLB33_00080"
     CDS             complement(14076..14504)
                     /locus_tag="PLB33_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOG61945.1"
                     /translation="MKLRDIMSREVACLSAEDTVEKAAQLMKTYNVGSIPICAQNSVI
                     GIITDRDIALRAVAVGDDGYLQVKKVMTANPVVGTPDMDVEDAVRIMSENQIRRLPVV
                     ENNRLVGIVSLGDISVEPRLQDEAEIALKNISKSATNRFS"
     gene            14646..15137
                     /locus_tag="PLB33_00085"
     CDS             14646..15137
                     /locus_tag="PLB33_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG61946.1"
                     /translation="MSIVKWNGRNLLKSIAENEKEATKLYRMFASEARIGEKFFELLA
                     KDEERHEKIYNALLEKYSDKLELEMEESDAEYMDLLVESNIGFDDELVEKAKKIFTKS
                     QIFDLAERAERDAVLFVTELQRLYPDLAKDEMAIILKEEKSHLKKVLERKKESQPMFG
                     RGM"
     gene            15247..15390
                     /locus_tag="PLB33_00090"
     CDS             15247..15390
                     /locus_tag="PLB33_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61947.1"
                     /translation="MADKNIKKENKKKKKKETKQANVSSVSYEQPVMSQPELVPKKKK
                     KDE"
     gene            15578..16750
                     /locus_tag="PLB33_00095"
     CDS             15578..16750
                     /locus_tag="PLB33_00095"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG61948.1"
                     /translation="MNILILNCGSSSIKFQIAEMPDYNVIIQGSVERIGYEDAEFKFF
                     LVKQKIIQPVLSHEEGINLIINTLFKSNFGIETKDINAIGHRVVQGGDKQNISVLIDD
                     KVIDYIESIKDLAPLHNPNHLAGIYACRKLLPNVPQVAVFDNGFHMSLPRSTYIYGIP
                     YEYYEKYRIRRYGFHGIAFRSMAEEVDKILNETHKNYKIVNLMLGSGTTANALKYGKS
                     VEVSTGFTPHEGLIQSTRAGDIDAAAILYLMKKENLSIEQADELINKKSGWKGLSGIS
                     NDMREIYQASQSGNERAKLTIDAVCHRFKKYVGAYSAIMEGIDILVFSGGVGQNAPYI
                     REKVCKGLEYMGIELDEYKNQNTAGIISKDSSKVKVIVVNANEEKIIAQDTYEICK"
     gene            16897..17547
                     /locus_tag="PLB33_00100"
     CDS             16897..17547
                     /locus_tag="PLB33_00100"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOG61949.1"
                     /translation="MNKNCFVSWSGGKDSCLALYKAIEMGYKPVKLFTMFGTEKISSS
                     HRLDENIIKLQADSIGIEYLIGKSDFDEYEKVFVSNLKSFKDQGIDYGIFGDIDLDEH
                     RQWEERVCKSAGMEAILPLWKRDRKEIAKEFISLGFKAKIVVINTSMIDIKFLGKDLS
                     LSLIEEIEKAGADPCGENGEYHTLVYDGPIFKKPVDIKYDYEIIPVGETWAQIKVY"
     gene            17613..17981
                     /locus_tag="PLB33_00105"
     CDS             17613..17981
                     /locus_tag="PLB33_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HOG61950.1"
                     /translation="MVDRFIIEFGMGVDFHGQDVTKAAVKAAKDAISKSCLIGLNEIC
                     GFNQDNVDDKVLIDVTIGVTRPDEVKIDEVVKCFPVGKVTVKAVYGGLKTEGLYFSKF
                     GDKDNSIEVAVASVSVKIKK"
     gene            17993..19381
                     /gene="lpdA"
                     /locus_tag="PLB33_00110"
     CDS             17993..19381
                     /gene="lpdA"
                     /locus_tag="PLB33_00110"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOG61951.1"
                     /translation="MKIAIIGGGPGGYVAAIRAAQLNAEVTLIEKEHIGGTCLNKGCI
                     PTKVLLQTSGEFEAVNKSFKDLGIKISGAELDWKQLQKRKTKIIRKLVAGVDNLLKNN
                     KVTKIMGEGSFVNKNQLKVKTADGAEIFVDFDYAIIATGSKPVVIPIPGVELPGVVTS
                     DEVLSLEEIPNSMVIIGGGVIGSEFAEVFSAVGCKVTIIEMLPNIIANMDRDIVETLV
                     EKFKKKGVEIYTNTKVVSISKAEKGLSVNTLSSGNEISFNAEKVLLSIGRKPVTDNLA
                     LENVGVETNRGAVLVNKNMQTNVPNIYAIGDATAGVMLAHVASAEGIVAVESIMNKKS
                     NIDFKTIPYCVYTKPELAGVGLTEEQAREKGYDVKIGIFPMYINGKAMIEGEQEGLVK
                     YVVDAATGEVLGLHMSGPNATELIVEGALAVRLEATVDEIISTIHAHPTVAESLQEAA
                     HAVHNNAIHIPK"
     gene            19455..19934
                     /locus_tag="PLB33_00115"
     CDS             19455..19934
                     /locus_tag="PLB33_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG61952.1"
                     /translation="MKLLVPVDGSNASISAVKKALELAKKYNFSIKLISVVSAKSSEY
                     RRHENAWKGIDSSVISESEELERQLEMKIKENAQRLLNAIISKLDFSGIRVELEVLLG
                     EPYVKILETARNDNIDMIVMSNRGFSKIKRFFVGSVTQKVISEAPCPVLVVNTGFEP"
     gene            19964..21295
                     /locus_tag="PLB33_00120"
     CDS             19964..21295
                     /locus_tag="PLB33_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG61953.1"
                     /translation="MRVVIIGAVAAGTSAATEIRRNDKEAEIIIYEKDKYISYAGCGM
                     PYYISNEAEGFSSVVPRDKDFFKEKHNIDIYTEHEVVSVNPNNKTLRVKNKSGEIMDR
                     YDKLIIATGAMSVKPDLKGSDRDNVFLLRNINDMIDIKNFIDENKPKSAVIIGSGFVG
                     LEMCESFKLLGLDVTIIARSKISKGIDNDLSLYIEEHLKEKGVNVYTNTKTVEINDSG
                     VVLGDGSIVKADMVLLATGAKPNVTLAKEMGVELGATGAIKVDKYMKTNIEDVYSCGD
                     CIEMYYSFNGNPVYRPLGSTANKTGTIAGSNITGIEQEFKGILGTAIYRVFELTVGMT
                     GLSEEEAVKSGYNVVITIDKRPNKPEYMGGRPIIIKTVAEKGTRRLLGAQLIGYEGVD
                     RRLDVLVTAITFNAKAEDLMHLDLAYAPPYSTPRDNLYYTGAKLKAKLSAL"
     gene            complement(21284..21835)
                     /locus_tag="PLB33_00125"
     CDS             complement(21284..21835)
                     /locus_tag="PLB33_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOG61954.1"
                     /translation="MYKEQTFSVRDITQIGIFTALTAVGAFISIPIGPVPITLQSFFI
                     LLSGIILGSKKAMFSQITYLLLGLIGFPIFSGFSGGLQHIFKPSFGFIIGYVAAAYVV
                     GKLTEEKTASQYLWAAVFAGTLIIYTFGLPYMYYILNIMLNSNLSIVKILQLGMFAFI
                     PGDMLKAVIIVFFAQKISPLLKG"
     gene            complement(21916..22890)
                     /locus_tag="PLB33_00130"
     CDS             complement(21916..22890)
                     /locus_tag="PLB33_00130"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG61955.1"
                     /translation="MSVKNNILHILESNKGKTLSGQELADMLNVSRTAVWKAINSLKD
                     DGYNIKSTPNKGYSLSESSDMLSAEGIRLYLNEEFKNIPITVYKTIDSTNAKAKLLAV
                     QNAEHGTTIIAEEQTKGRGRFGRDFFSPAESGIYMSIILKAELSIENSVLVTTAAAVA
                     VCQAIEKFTSVYPKIKWVNDIFIGDKKVCGILTEAVTNFESGMMDCVVVGIGINVKTK
                     NEEFPLELQNTAGSIFIDDDNYIRNQLAAEIINNLLKAANNLADKSFMKSYKQRSMVL
                     GENILYKKDNKWHEGYASDIDDYGGLVVYTEDGQKITLNSGEVSIKKK"
     gene            23102..24013
                     /locus_tag="PLB33_00135"
     CDS             23102..24013
                     /locus_tag="PLB33_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOG61956.1"
                     /translation="MILPSEIRLCEVGLRDGLQNEPKILTTEEKLKLAEGLINAGFKV
                     IELGSFMSPKAVPQMADTDELFRHFNNVEGVELRALVANKKGVERAINCGCGKIKLNV
                     SASKKHNLANINKTPEETVAGFAENVDLALENGLEVSGSISMPFGSPWEGVIPIKDVE
                     EIVEAYLRVGIKEISLSDAAGVASPRQVYDICSSMQKKYPDVKWWLHFHNTRGMAMAN
                     ILASMEAGITNFDSSLGGFGGCPFVPGAAGNVSTEDVVHMMDEMGIKTGIDILKLIEL
                     SRNLQDMLGNEGDSYILKAGRTKDLFK"
     gene            24083..25552
                     /locus_tag="PLB33_00140"
     CDS             24083..25552
                     /locus_tag="PLB33_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOG61957.1"
                     /translation="MSRMSSMDEAYAKLTQDQIDEIEEKFAKADAAQKIFEKWSQEDI
                     DRTIRAIAWKVANTQTFKELVKMSIEESKLGDPVSRENKKYKIRGVLRDALRQKSVGI
                     IEEIPEKGIVKYAKPVGVIACVIPATNPDLTPAGNAIYALKARNAIIFSPHPRAAKTG
                     GRTIELMREGLRQVGAPEDLIQILGQGKAKINRAMSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAYGVGAGNATMIWDETAKDELYKAAYNTMLSKTSDFGSGCSADGNIVMHESI
                     FEEGINALIEAGGYLLNESEQESIKKIMWDEECHRLPDSVAQSPQRMAQLAGFEIPED
                     RKFLIAMGTGSYTTAYEDVWCREKLTTLLAVHKYEGEFQNAIDIIKAIHEVGGKGHSV
                     GIYSYDEDHIHRLALEAQVGRIMVRQPQSKSNAGSFTNGMPMTSSIGCGTWGGNATSE
                     NVHLKHYMNVTWVSKEIPEDRPTDEELFGEFYEPEREGK"
     gene            25568..27385
                     /locus_tag="PLB33_00145"
     CDS             25568..27385
                     /locus_tag="PLB33_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOG61958.1"
                     /translation="MKRLVADQMVEYLERRNVEYIFGLCGHTVIGFLDALSRSEKLKY
                     VSFRNEQLAAMAADGYARIKKKAGVVLCHLGPGLANTVTGVANASFDSIPMVVIAGDV
                     PRYYYGKHPHQEVNMHADASQYEILRPIVKRAWRIDDAEALPEILDKAFRLAESGRPG
                     PVLISIPMDVFSVKIDEELFNRTFKDSSETIKPALAPSAAKAIAKKLIEAKNPVIHAG
                     GGILLAGASEELRQLVEFLDIPVSRTLMGQGCLSDTHPLMLGQTGFWGLEFTHKFTAN
                     ADVILGLGTRFAEADSSSWYTGVTFDMNKTEFLQIDIDPAEIGRNYPVSIGAIADLKL
                     ALAQILEEAKALVPEGIKRPGLRDMIKANRDEFRKSNIEVSTDGRFPMTPQRILRDVK
                     SVIPEDAVIFTDVGWNKNGVAQQFDITIPGTIHHSSGLATMGFGGSALIGGKLAAPDK
                     VVLTLVGDGGFGVNPGALATAVEQNIPVVWVVMNNSAFGTIAGLEYSNYQTKFGTVFT
                     TPDGKPYSPNWAEVAKAYGVDAVKIKSADDFKPELEKAIKSNKPYLLDVPMENIAVPT
                     PGSWNINDIYNPKEGVVNGKLVKNEKGEYMPPDHSTSHK"
     gene            27407..28252
                     /locus_tag="PLB33_00150"
     CDS             27407..28252
                     /locus_tag="PLB33_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOG61959.1"
                     /translation="MKRKFSLAYLTIPGTHPVDQIRIAAEAGYDFVSLRPIPMHLPNE
                     PLFQFDLDRKLFKDIRDALEQNDIKLMDIELARVREDLKVEDYESAFAAAAELGATDV
                     LSSIWTKDKEFAFKEFAKICDLAAKYNLKVNLEFVTFSGVTDLTGALEVLDKVGRPNA
                     YLMVDTLHAHRSRVSPEDLAKVDRKKFGFIHLCDGPGEIPSLEDPSMIGVAREGRLYA
                     GEGEIDLKGMLLAMPDNPISIELPNSKEMKERGAAGHATRCLITAKELLVNMAKEEDI
                     ECQSI"
     gene            28237..29118
                     /gene="aroE"
                     /locus_tag="PLB33_00155"
     CDS             28237..29118
                     /gene="aroE"
                     /locus_tag="PLB33_00155"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOG61960.1"
                     /translation="MPVNIKTKMVTLLGKPLSQSYAARMQNAAYRAAGIDMLYFYTEV
                     ENDHLPDVVNGIRYMNFAGFAVTKPNKVEIMKYVDEKDPLCEKMNASNTVVILPDGRL
                     KAYNTDGIGFFRALKEEYPNTKIEESTFFCIGAGGAGRAICSVLAYYGAKKIYIANRT
                     LSKAQTLVDDINRNFAPVAELVDLNDTNLLKEKIKESDVVMNNTGLGMPSSLDGTPIP
                     KEYLHSGQLCFDATYNPSKTRFLIEAEEMGCSIMNGLGMSLYQGAEQIELWSGKTAPI
                     EAMRQELMNILAGKKEE"
     gene            29149..29727
                     /locus_tag="PLB33_00160"
     CDS             29149..29727
                     /locus_tag="PLB33_00160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOG61961.1"
                     /translation="MSAIKWLDEHLEEAIMVIMLALISCVMMAQIIARNVFNSMTWPE
                     EFSRYCYIWTVFLSLGYTIKKGNMLKVGLVMDLLPTKIRRSIEIVANLIILAICVVFF
                     RHAITYTGKIYNMGQISPAMHLPMWIMYLATVTGFGLASIRAAEEIVKNIKNFNVKVE
                     TTIEATLKEAMEEVETTGINVDDELKDSGGDE"
     gene            29727..31010
                     /locus_tag="PLB33_00165"
     CDS             29727..31010
                     /locus_tag="PLB33_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOG61962.1"
                     /translation="MALWIFVVFMVALIFAVPIAMSMVLGALTPLFLGGTGSSIQQLI
                     ANSFSGSDSTPILAVPLFILAGVLMAEGGISRKLFNFFAYFIGNFTGGLPCAVIITCL
                     FYGAISGSGPATTAAVGSMTIPFLVEMGYDKVWAAGMIATSGGLGVIIPPSIPFVLYS
                     LATGVSTGALFLGGVLPGIAIGLGMMVYAVYYCKKKGEDKEKINAKMKELRGKGFFNL
                     FKESFWALLSPVIVLGGIYSGYVTPTEAACISVFYSLFVSLIVYKSFTVKEIIPFLAK
                     AVKTYAPLCFVLAFAIAFGRVLALAKAPAIIEGFILDNFTTSITVLTGIVIVFLILGM
                     VMDTGPAIVILSPILLPAVQALGVHPVHFGVIMVCCLSIGLATPPFGLDLFVAGTLSD
                     TPPMTVAKKAIPFIIAFGIVLFVITYVPWLSLALL"
     gene            31050..32138
                     /locus_tag="PLB33_00170"
     CDS             31050..32138
                     /locus_tag="PLB33_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOG61963.1"
                     /translation="MKKLLILLLIATMALLPVACTQPSTPTPPEQPEQPEQPEQPEQP
                     EQPGGFDALEPVVLIGADNAGVGAAAQLYGELITEKVKEITGGKLTIDYFPNSQLGND
                     QELQGQMLAGDIDFVICQTAQTVSFVPEVAIFDLPMVFAKYDAETIDYALNKSEFTDK
                     INQAYKAKNMYCLHFLQGGTFRETTSNKKIETIDDFNGLKIRTMENKNHMAFWQAMGA
                     APTPLPWPEVYISLQQGLVDAQENATDTCVGANLQEVQDYLILTHHILYCNQFLINAS
                     KFDSLDPLYQEALQQAIDEAAVEIEAQLTEIDVTNRQTLVDGGMELVEFDASFIDQVL
                     EKAKGVYESIGNDIGQEYVDSLLNALEK"
     gene            complement(32283..33158)
                     /locus_tag="PLB33_00175"
     CDS             complement(32283..33158)
                     /locus_tag="PLB33_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG61964.1"
                     /translation="MTLQQLHYFRVLAKVENYTKAAGILHIAQPSLSYSISQLESELN
                     IKLFERHGKKIKLSNPGELFLKYVEKSLDILDEGTIMLKTVADSSMGEVSLGYIYSIS
                     SKFIPEMIKKFYENNSNRLIKFSLVQNLGEQLIRDLKSNKIQLAFCPTPDKSLKCVKI
                     FSQQLHLIVPKKHPFSQRKCIEISDIIDEPFVALNKSSSLRSTVDEIFARYNFSPKIV
                     FEAEECNAVISFVSLNFGISIVPEIPSMNKHVNTVKICNMPLKREIYLCWIDDKYLSP
                     AAIKTRDFIIENFTV"
     gene            33560..34459
                     /locus_tag="PLB33_00180"
     CDS             33560..34459
                     /locus_tag="PLB33_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG61965.1"
                     /translation="MESVNKYKYMGFILLQSVIYGFGNPLTKIAFKSITPFWSLSVRF
                     TIAFIVFMLFFGRRIIGSLKSVEPKVYLPAGICMATAYITCNLALSWTSATNVGFLMS
                     LPVIFAPILALLIYKVKYRLIHIPVQIVVVIGLYLLCFDGEKLVFGKGEITALITAIS
                     IAGALVFGEESLKQLDAVAVSAVQAGITALISLPCALIFENTEILVHVTIPAWLVVLY
                     LAIFCTNIAYMLQNSALKYISASTVSMLQCTQPILTAIISYLLLREKLSTLGIIGAAI
                     IILCIIAENIITTKEQSASKNLV"
     gene            34515..>34828
                     /locus_tag="PLB33_00185"
     CDS             34515..>34828
                     /locus_tag="PLB33_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOG61966.1"
                     /translation="MFIKKNQFYIIIAVIVFLMAVYMIYSIRKDNRLNNNEENVIKLV
                     EDFGSTLKNVPLLAPRDEVIQAIKDNYSPYVTDELMQNWIAHPRRAPGRTTSSPWPDR
                     IE"
     assembly_gap    34829..34928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35001..35456
                     /locus_tag="PLB33_00190"
     CDS             35001..35456
                     /locus_tag="PLB33_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOG61967.1"
                     /translation="MAGLVPYNRRNRGLVDRGFSDFRDFYNMLDDFFYDAWPSKKYFA
                     RDTFKIDVQENDNEYLVEAELPGVNRDEVNIEMNEGRLNISIQREESINEENKNYVHK
                     ERRFSSMSRSLYLDDAKSTGIKAKLDNGVLSIIVPKENKPNKSVKIDIE"
     gene            35704..36474
                     /locus_tag="PLB33_00195"
     CDS             35704..36474
                     /locus_tag="PLB33_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG61968.1"
                     /translation="MSEVLRLEHVSKSFGSKDVLKDINFSVNSGEIIGYIGSNGAGKT
                     TTIKIILGLIDDYQGDVFIFGEKVKGQFEYKKRIGYVPEVSDMYDNLTAYEYLSFIGM
                     LYGIGEDKAVKKGKEMMSVFGIDDAINGRIHTFSKGMRQKLSIITGMIHNPDILFLDE
                     PLGGIDANSVLVFKEVLKNLKKEGKTIFYSSHILEVVEKLSDRILLINEGEIVIDGTV
                     ADVMSMQSDSSLESIFNYVTGFHDYEMLAKKYTDVFMS"
     gene            36486..38099
                     /locus_tag="PLB33_00200"
     CDS             36486..38099
                     /locus_tag="PLB33_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61969.1"
                     /translation="MNEFLSLKILDSFKRIFEGFCIDYKLLRIILKTKLILDSRRAPV
                     INTNQKKKDEEKVSLLKGYFMHLLFGAFLAFFIYFIKDEMIRMMYFSGAFFFMTTMYL
                     ISDFSFVLLDTKDKNILLTKPVNSRTVSLAKVLHILIYMFRLNTFIAGPSLIALVMSN
                     DLFVIPIFLVEILLMDLLIFLVTAFIYLLVLRFFDGELLRNIINFIQILLTILVTVGY
                     QIAIRMIDFTSLRNMEYTFNSRHFLNPILWFGAPFEIFYHGGESYLYVFTALIIIVPL
                     VSFIVYLSLSKKMESLLLKLEGEGKERPSIHWIEKIVGFIINPNEISKQSFNFANAMF
                     RSDRSLKLKIYPALSSGILLPILMIFNMRMGGREFKPSGYEYLYLYFNLLIFSSVFYL
                     VKYSSAWKGSYVFISSGFKDYKNLYRGLMRSMAYRFFIPLIILNGVVFTLIFKFRFVE
                     DIVILFLAGIAILPLIGKIYLEIPFSKPIEESNQRDSFDKFLLSFAIVAIFAIVHFYS
                     NIIPMGKWIYLSVLAVGIPISWKFATPGE"
     gene            38118..38693
                     /locus_tag="PLB33_00205"
     CDS             38118..38693
                     /locus_tag="PLB33_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HOG61970.1"
                     /translation="MDKVTLWTRQDIRSLDDLERKGVIRITRKHLEEKFDVISDHIIF
                     LYNWFVNEAEKRVPRPEGVEFFIWCSISEEYMLRPTLNEIVYVLEVDKSEVVYFDGMK
                     WDYVLNHHYVPKDEKDAEEYLKDLERKGFKNSYSFINEKTAHFYPQERKRVMDSWARV
                     FEIDEWDIFRIQANIWEIRPEMIKDILRADS"
     gene            complement(38690..39310)
                     /locus_tag="PLB33_00210"
     CDS             complement(38690..39310)
                     /locus_tag="PLB33_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOG61971.1"
                     /translation="MTKYKSDNVHNRTMTLTGHSEMEIAPDIVIIRLGVQTTGEDLSQ
                     IQADNARISQSIIQTLQRMGITDIKTSQYSIDRVYDFEDGRQIDRGFSVRNILQIRTT
                     NLEAAGSIIDAAVNAGANVVDLITFDISNREYYYQQVLNMAILNAIQKANSITMNLGY
                     STPALVVNIVENAAIPFLPVQREFAATPIIPGTIRIEANVTVDFVF"
     assembly_gap    39380..39479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            39626..39997
                     /locus_tag="PLB33_00215"
     CDS             39626..39997
                     /locus_tag="PLB33_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG61972.1"
                     /translation="MKFLWTTILVNNMDESLKFYEEIVGLKMTERFQAGPGMEISFLG
                     EGETKVELIYNEKFKKLDAGNRVTLGFKVESLDEKINFIKEKGIKIITGPIEPNPSIK
                     YFIIKDPDGVKIQFAQQLQSN"
     gene            40181..40909
                     /locus_tag="PLB33_00220"
     CDS             40181..40909
                     /locus_tag="PLB33_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG61973.1"
                     /translation="MLRIAICDDDKELCIELKSMLNEISKNTGEAFEISVFCNGEGLC
                     DSLMSGNRFNLIFLDIELCEINGVEVGKKIREELNDEITQIVYISAKESYAMELFDIR
                     PLNFLVKPLKIEKIKYVLKTARKVLGSSNQYFEYRVGNVNFNVLISDILYFESSGRKV
                     KVVLKNEVNEYYGKLSEVEEKIKNDDFFFIHKSYLINYNHVIEYSYEYVKMSNNEILG
                     ISQNNRKAVREKLLRKKQRMRHVK"
     gene            40899..42170
                     /locus_tag="PLB33_00225"
     CDS             40899..42170
                     /locus_tag="PLB33_00225"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOG61974.1"
                     /translation="MSNSYVAVYLITNMFATYTLYRYMCIFFDRKDVDKRQEFLSYSL
                     YFCINSFLYLTFNKPALNIASNLIMFFLLTYNYQSTTKTRLIAVVLIYMILMTIEVLV
                     MLILQNYGITVTSGNDAALITGLISIKIISYIFMLFMSNFKMINNEIKVSNIHWLSIF
                     IIPVGTLILVLIMISMDYASNVTGIIISIMILLIINVFVYYFYDELMKSYQSKIEKTL
                     LQQQNNAYLKQLEIINQSKENIKTFRHNVKNHALTLKYYIDNHDRDGAIKYLDNIFEF
                     INYSKEHAKSGNFEIDSLINYKMDLAEKNNIKAEVYLAIPHKLNINPFDLNIVIGNLL
                     DNAIEAALKAENKFINISIELDRNVLYISISNSYDGRLKLIKNKLVTTKDNDNHGIGL
                     SSVQNSIEKYNGTMNIHYDERVFYVDVLMYN"
     gene            42322..42720
                     /locus_tag="PLB33_00230"
     CDS             42322..42720
                     /locus_tag="PLB33_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61975.1"
                     /translation="MNFEEYIRKVYTIAFRLTGDETSAKDVASLAIDKNANIVKENID
                     SNILHITIKEVCGIFLKEYSIFSSESFYNKNNTTIQNKLMNLEPLSRTAVVWKDIMGY
                     NMKDLETISNCPKSELYEKLNKARKNLIHF"
     gene            42803..43480
                     /locus_tag="PLB33_00235"
     CDS             42803..43480
                     /locus_tag="PLB33_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003207775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOG61976.1"
                     /translation="MKYKCLILDHDDTAVKSTPNIHYPSFVEALKSLRPDIELSLNDF
                     VNYCFNPGFSLLCKDILKFSDTEQQYQYKIWKDYVNNNSPEFYSGFPELIKEYKSLGG
                     TICVVSHSESQHIIRDYTKHCGLKPDLIFGWELDEHQRKPNSYPIIETIKRFNLRNTE
                     MLVLDDSKLGLDMARSCNVEFAAAGWSHSVPGIEKYMRENSDYYFSTIDEFRKFILDC
                     GNLYDFK"
     gene            43596..43823
                     /locus_tag="PLB33_00240"
     CDS             43596..43823
                     /locus_tag="PLB33_00240"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOG61977.1"
                     /translation="MSVEKLLLSNLSTIEAEIVIAKLKSYGIPVLKKSKGTGELMEIY
                     TGANMYGIDLYVPSDMIELAEELLKPENETE"
     gene            44267..45157
                     /locus_tag="PLB33_00245"
     CDS             44267..45157
                     /locus_tag="PLB33_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG61978.1"
                     /translation="MKKYKVWILLTSCVLFWSGNYVFGSLVVTELTPVGLTFIRWLFA
                     SVILLIVGQKIEKPDWKEVCKQWPKITMLSMTGIIGYNIILYYALYYTSPLNAALVNS
                     INPSFIAIVSAFYLKERITKYHVFGIMISFLGVWIVLTKGNLLQAFNMKYNRGDLLVI
                     AAVITWTSYSVISKKLTTVPPITSTGVSGLMAGIILFPFAMTSGVNYLNMSTLALTAV
                     VYIILFPSLLSFVFWNIGVREIGAGKTGIFLNLNPVFTAFISRAMGKDITWVQVIGGL
                     LVFTGVYITTAFKGKTARLK"
     gene            45168..45683
                     /gene="cobO"
                     /locus_tag="PLB33_00250"
     CDS             45168..45683
                     /gene="cobO"
                     /locus_tag="PLB33_00250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG61979.1"
                     /translation="MEKGYIHIYTGNGKGKTTAAFGLAVRAALSGKKVFIGQFVKNME
                     YNETKIKDYLSNIEIKQLGRGCFIYEDPEEIDKETAKKGLEECSTLLASGEYGLIILD
                     EINIALYFKLLEVSDVVSALKNKAENTEVVLTGRYAPKELIEIADLVTEMVEIKHYYS
                     RGVLSRDGIDH"
     gene            complement(45736..46539)
                     /locus_tag="PLB33_00255"
     CDS             complement(45736..46539)
                     /locus_tag="PLB33_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG61980.1"
                     /translation="MKKSEIKPEEGKKPSPKSPKYTEVNSKFIDKWIDEGWEWGQPVS
                     HEVFERAKNNDWFVLLTPTKPVPKEWFCEMKNAEVLGLASGGGQQMPIFTALGAKCTV
                     LDYSEKQIASEIEVAKRENYEIKTIRADMTKPLPFEDETFDLIFHPVSNCYIEDVLPV
                     WKECFRVLKKGGILLAGLDNGINFIFDDDETTPVRKLPFNPLKDEELYQLSVEKDWGI
                     QFSHTIEEQIGGQIKAGFIIEDIYEDTNGSGRLHELNMPTFYATKAVKK"
     gene            46747..47121
                     /locus_tag="PLB33_00260"
     CDS             46747..47121
                     /locus_tag="PLB33_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG61981.1"
                     /translation="MNIIISNSSGQPIYEQIASQIKSMIITGELREGEALPSMRLLAK
                     ELRISVITTKRAYDELEREGFIVSFTGKGSFVAGKNIELIKEQHLKEIENHMQEILKL
                     SKECGLKLDEMIEMMTLLYEGE"
     gene            47123..47971
                     /locus_tag="PLB33_00265"
     CDS             47123..47971
                     /locus_tag="PLB33_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG61982.1"
                     /translation="MEYSLKLENVTKVYKDFKLDNINITLPKGSIMGFIGENGAGKTT
                     TIKLILDLISRDGGNISILGKDNKTEMDSVKENIGVVLDESSFPENLNAREINNMLKK
                     VYKTWDEGKFSKFLDMFSLPYNKSIKDYSRGMKMKLSTAVSMSHDSKILILDEATSGL
                     DPVVREEILDTFLDFLQDETNSIFVSSHIISDLEKICDYITFIHKGKIIFSETKDDLL
                     DNYGILKCSPEEYENIDKSLVKGMRKNKFGIEALVLKDRITGPYLIDRASLEDIMIFI
                     IKEQVQ"
     gene            47968..48579
                     /locus_tag="PLB33_00270"
     CDS             47968..48579
                     /locus_tag="PLB33_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOG61983.1"
                     /translation="MTGLILKDLLNLKKQGKVYLILIIFYFAIGLVNENSSMFASMMT
                     MVAVLIPITAMAYDERCKWDRYALTMPVSRKDMVMSKYLLGLIFLTAAFVISILLNMV
                     FSNMLINEKILTALGNFFAGLTLMAVIFPLLFKFGVEKGRILMMIVIFIPVAAVMMIS
                     KIGIPMPAEEKLEQLIYFLPIIGVLLFIISIYVSLRIYRKKEF"
     gene            48626..48892
                     /locus_tag="PLB33_00275"
     CDS             48626..48892
                     /locus_tag="PLB33_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOG61984.1"
                     /translation="MKKVYEFDAVIKKVPDIDGAYIEFPYDVKEEFGKGRVKVYATFD
                     GEPYVGSLVRMGTPCHIIGIRKDIRAKINKQPGDIVHVTIRERS"
     gene            49074..51416
                     /locus_tag="PLB33_00280"
     CDS             49074..51416
                     /locus_tag="PLB33_00280"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOG61985.1"
                     /translation="MDFKKFMVLFLCILLFASCSDNNVVKEPEPVEEPEVIEEDNIPT
                     AWNLSMEEFRVDVPFSVPDVIPVVEKYEVNEDLSNLVNAGQYAGFTDKQLKSIYEDGF
                     VVLKPSYEYLKMHHLYEYPMYKESPVFITVDSALHLYHIFYGNSLKLLEVSSLYDKLQ
                     SLSKNMLTESLNAYNDSKYANLKEELKFAAAYFLTGAKLIDEDLEGIVVPEEIAVLSD
                     DEIKLIDEAFDFARSPIFGKDLDYSQFTVRGHYTGNEELGQYFKTMMWYGLSGFPIFD
                     ESKSKPVLDMDSLTKSMIITCLLLRNEDSFDDFENIYTATALYTGMSDDLGIFEIRDL
                     ITKVYGQNPDLNKFKDNSYYDKLLEEALLLPEPKIQPKYSSVSTPAGRQFRLMGQRYS
                     FDAEVMQALIEPIIRPIPSGLDVIASFGSKRAEELLDTYYKPKEDWDKYEENLNLMRK
                     KQTEITDDEWKSDLYKGWLWSIKSSAVSFEDKEGMPHFMRNEKWTDKNIHTALGSYAE
                     LKHDSILYMKQSGAEMGGGPEPIIPYNYVEPNVEVYAKLKWLAENTKAQLQERNMLKD
                     EIGLVLDQIIDIQDTLMNVSVKELTNQDITDEENLKLYRYGGLIDSVIQIMQMNLMRN
                     DVDTSNDFTTALIADVSTIAPNDLFPKGTYLEIGNGLPCEIYVVCQTNGKTYLARGAL
                     FNYYEFLSDKRLTNHEWQTLVGVKRMAMVYDEEKNIHVPMDIYDEDGNRILEEDEYDF
                     ENIMIIGPSENMVPKPAWTESFISQEENKVTIKDISISWE"
     gene            51418..52140
                     /locus_tag="PLB33_00285"
     CDS             51418..52140
                     /locus_tag="PLB33_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61986.1"
                     /translation="MKRLLIPLAVIMAGAVCVLIRQPESYAAGKISIEQNTYGLYEKS
                     NYLYIKSGKKYVKIIDEEIIDFKTYDIDSDKDDEILAISKHISEEYGSDFIIYDIYEA
                     DGIILTKEIYRQDFFDFKPWKVDACNLDNDGETDIFIGVYKDTVFYKDLRKRPFFYSW
                     DGDRLHKKWLGSFFSDWDLVDIAFGDFYNLGYDVAAVLEKNKDACRIGIYQFIGFGFE
                     NMKTINSVDNSFRSQTELLFGK"
     gene            52241..53467
                     /locus_tag="PLB33_00290"
     CDS             52241..53467
                     /locus_tag="PLB33_00290"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG61987.1"
                     /translation="MKRFFTRKKVIVFVVVAALAAIIFWLVNKGSKAPEVTANVSKLE
                     RKTLEHVISVKAPLVGIEKAEVTSPLNYKINDIRVKEGDLVKKDDILAVLDSEDLEKE
                     IEYEENQIELSKMEAEEKIKALQIEYDKALASYRDMETVYEQNKDLYENGVITEEAFK
                     KTEKDLVVLKKSLEGYNAVNGKIQLSQADKKRIEIQEDRLKLKKEELEKVMIRSSIDG
                     TVTRVNVNIGRYAKDTENSQAMFVVENLEKLQMKAAVSEFDVGKLKLNQEVEIYSDIL
                     GKGFAKGKVARISPTAEQKDNNTMERVIPVLIDVTERPENLIAGVIATAKIRTEKKEN
                     VFAVPSGAIVQDGEEYKIYILNEDNTVRSIPVEIGLETDLESEITGDLYEEMKYVINP
                     DETFEDGMTVIPNEIE"
     gene            53474..54181
                     /locus_tag="PLB33_00295"
     CDS             53474..54181
                     /locus_tag="PLB33_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG61988.1"
                     /translation="MEKMIRIENLNKIYKNGSVEVHALNDVNMEVSRGEFLAVMGHSG
                     SGKSTLMNIIGCLDRPSSGKYFLDGIEIDKQSPDELSLIRNKKIGFVFQAFNLIPRTN
                     VLSNVELPMIYAKVKSKQRSERALELLEKVGLKDRINHMPNELSGGQMQRAAIARALA
                     NNPPIILADEPTGNLDTNSSEEIMKIFKDLNREGTSIIVVTHEPEVAKYADRIIVFKD
                     GCIIEDRWKEGGRNYLA"
     gene            54099..55376
                     /locus_tag="PLB33_00300"
     CDS             54099..55376
                     /locus_tag="PLB33_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG61989.1"
                     /translation="MQTELLCLRTDALSKTDGKRVEEIIWLENIKIAFKSILSNKMRS
                     LLTMLGIIIGISSVISIVSIGDSMKGVMDDIYKNFGKNRAVLYVGNIEDFRTTDFFYN
                     DDLEILKERFSEKLAYICPSDSLRSDVTAGRNTIKLNMEFIDSGYDKVQQIKMLYGRM
                     INSKDIERKSKVVVLEQKAALKLFGKDNAVGKTIRVKIDNNMEDLLVVGVYKIEQSPL
                     LTLMQGQSNYENSFIPYTMPTSSYYAYYALDIFIDEKYSMAITGDEIINYIARMKNRT
                     PENYIYYTVEEEQTQVESVVTGLSVAVAAIAAISLLVGGIGIMNIMLVSVTERTREIG
                     IKKALGARTKDVLFQFLIESATLSAIGGIVGTITGIGLVMIGGSFISIPVVVNPASII
                     LAVVFAMVIGIFFGYYPAKKAANSDPIEALRYE"
     gene            55407..56780
                     /locus_tag="PLB33_00305"
     CDS             55407..56780
                     /locus_tag="PLB33_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOG61990.1"
                     /translation="MILSEEQKNHYKRQIIIPEIGEQGQKKLLDSTVLVYCKNTDSLR
                     PLAYYLAAMGIGKIFCDLQDKIGADSLFAEVIDLNNDTKIDYLNEENYSGGFSEIYEN
                     NKNHPQNFCRIVLGNYSFIKNSAAKLLNDKYLPTIISANSQWKGTLQTFINEDLFKNF
                     IALIADNNTKDFSPPFLANALSATLSSIECVKLCLNIGNITKDMLFFDLFNMEFKQTD
                     NNANLFEFLNEANKMDIKQKLSDAKVLIVGVGGLGSPAAYGMAMADVNTLGLLDLDEV
                     EMSNLNRQILHSFSRIGMPKASSAEFMLKKLNPSINIKTYKTELTRDNAEKIFLEYDL
                     IIAAVDNIPSRYLINDICFLLNKPFAEAGVLRFDGTATTLVPKEGHCYRCLYPNVDSS
                     NLSGNNGVLGSVPGVMGFIQAAEAVKILSGFGKTLKNKILLFDSLAMDFNIIDIEKNP
                     RCPTCGK"
     gene            56907..57350
                     /locus_tag="PLB33_00310"
     CDS             56907..57350
                     /locus_tag="PLB33_00310"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG61991.1"
                     /translation="MKKFKKFTIIFCALMLLLVACAPGDGNLNEDEDNEPIDEPIDDN
                     NTGANANNDTFDEEEKTLTGSAQGYGGEINVTVVVNGEDIVSVEAEGKDETEGVGSLA
                     IDELPDKIAQANSTDVDNVSGATKTSEGIKQAVNNALQSNKQSKQ"
     gene            complement(57387..57821)
                     /locus_tag="PLB33_00315"
     CDS             complement(57387..57821)
                     /locus_tag="PLB33_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOG61992.1"
                     /translation="MDFLDMQIIKHLEKEGRISHEELSKRLNLSRPAIHNRVAKLEDQ
                     GIITGYKAMIDWSKLGFTTYAIISLRVRTADYDKLLEQLKNLAIPDLVIEECHIVTGT
                     WCILMKVRCSAPSGLKAIQDELHKIECIRESSTSLILSSLYD"
     gene            58096..58656
                     /locus_tag="PLB33_00320"
     CDS             58096..58656
                     /locus_tag="PLB33_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61993.1"
                     /translation="MKNKIILILICFLLFITGCAVNDNPPEKETEPYGKYTANGRIVK
                     IDKDGFHVEGEDNVGLYKVDMEKNKNFYIGERVRLNSMDGNSYDVELDEEYDYTAAMT
                     AEIFDDDSKLDLKVGEISRDEMGRMRIYGLAADDKEYDIIVGADTITNFAHSTLKVGD
                     NIYVYPKDVPNGIPAVVDAKAVTVVR"
     gene            58788..60305
                     /gene="hutH"
                     /locus_tag="PLB33_00325"
     CDS             58788..60305
                     /gene="hutH"
                     /locus_tag="PLB33_00325"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOG61994.1"
                     /translation="MDKVIITGNDLTLDEVVAVCRNYKKVELSDFAVERILKSRKVVD
                     DFVENEDVVYGITTGFGKFSDVTISKEESKTLQKNLIISHSVGAGKPFDSEVVRGIIL
                     LRINNLAKGYSGARLETIQTMIEMLNRKVHPIIPEKGSLGASGDLAPLSHMVLPMIGL
                     GQAEYEGEIMAGEEAMEKAGIPVIELTAKEGLALINGTQVMTALAALTVYDSLNIIKT
                     ADIAAALSFEAQNGIIDALDQRLHDIRPHKGQIDTARVLTELLTGSKMTTKQGEIRVQ
                     DAYSLRCVPQVHGASKDAINYVKEKIEIEINSVTDNPIIFPETNEGISGGNFHGQPMA
                     LSLDFLGIALSELANISERRIERLVNPTLSGLPAFLVEHGGLNSGFMIVQYSAAALVS
                     ENKILAHPASVDSIPSSANQEDHVSMGTIAARKAKEIMENVRRVLAMEIMCACQGIDL
                     RGNKGLGAGTKPVYDEVRKVVPMLKEDRPIYDDINKCEELIKNGTITKACEGRIR"
     gene            60435..61016
                     /locus_tag="PLB33_00330"
     CDS             60435..61016
                     /locus_tag="PLB33_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG61995.1"
                     /translation="MNNNERWLVEESRKGNVEAFEELIKDYKKTAYNIALRIMRNVED
                     AEDASQEALIKIFKNISSFNMESTFKVWMYRIIVNTCIDFKRRKNISTVSIDETMDLG
                     SGREVQREISDESYNPDALIERNYSTQLVNDAINALEDDFKTIIILRDIKGFTYDEIS
                     QILSCNLGTVKSRLSRARKRLRELLENEMKVQG"
     gene            61029..62300
                     /locus_tag="PLB33_00335"
     CDS             61029..62300
                     /locus_tag="PLB33_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOG61996.1"
                     /translation="MKCNEINDKLSLYIDNELSHEEMQQVEEHLHSCEKCQKILEEYK
                     NLISVLRNLPEEEPPVGYCKRLHEKLLESNPQSKTKKAASKIKSFNRNKWLKYGSLAA
                     ALVLVFVAANINNIGRMGMKAKNESYDMATTESPAEYYGAGSPAPEAPQAGPPVMTEE
                     AAEEGYDGDNKSARDDLSYRTNDSVMMTSMEQREMKIIKTGTISAQTEDYDMFLNDLS
                     MKVSSMDGFLESNNTQVYQVYNEKKLMYGSIRIRVPQESFYELIDYLENTVDIKRKNI
                     NETDVTKEYYEKDNKVKNLEVQEQHLRELFEKATTVEEMLLIENELRRIRTEIDALNI
                     SLSDINDRAKMSTINLEIEEVMTASLTLKTKDSVWDRSKEGFINTVNGIIRAVENLIV
                     GIVSSSPILVPAIIICIVGFLKIRKYWHRKI"
     gene            62604..63575
                     /locus_tag="PLB33_00340"
     CDS             62604..63575
                     /locus_tag="PLB33_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG61997.1"
                     /translation="MIKTNKTIAELKPGEKADGFYLIKSYEVKKTTNGKQYIDIELVD
                     KTGEINAKVWDYSKETEVLIEKNPIVKVRGEVLEWSGTRQLKVNKIREKKAEDNVQIQ
                     ELIPAAPIEPSEMLEKVKSYINKIKDNEIRTLTDNIFKKYENKLYYYPAAKRNHHSYR
                     GGLLYHNLRMLQSGEKLCQVYDFLNTDYVYAGIILHDMCKILEMESDEYGIVSDYSME
                     GKLLGHIIQGVKEIEFEGEKIGIDREKSIILQHMVLSHHFEPEFGSPKKPMTPEAELL
                     HFLDIMDARMFDFEHFLEMVEPGQFTDKIWLLDNRNLYKLKGDNKDS"
     gene            complement(63600..64247)
                     /locus_tag="PLB33_00345"
     CDS             complement(63600..64247)
                     /locus_tag="PLB33_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG61998.1"
                     /translation="MENLIGMIRTFAPLTSPQTVSKVNTYLPAVEKASTLLGMYSFIS
                     KAQNFRPIQAIDAKTPADKVTALLKNGNIPVTKLMAQPLIAENMQKIVSALAMNMAKN
                     GGLNEMLSSLTNSNNNNLSDMLSSFSDGNKENTDLSNLIEKFMPMINNVMSSSNKTEE
                     PNKPYEEEVFKKAQFDHKKNDEINNKTINTPPSAEKNIRSEKHIQKPIRIRQRRR"
BASE COUNT        22229 a         9802 c        13258 g        19028 t
ORIGIN      
        1 attttcacga agtgttatca atgcaccatt ctttagtttt attgtggcaa tgatcttgta
       61 caaggttcga aagcccggcg cagtatcttt atttggttta ggttatggag ctatgatggc
      121 aagaatgtca atattcggga cattgtccat agcagctgga ggaattgtcg ctgatattat
      181 aattagtctc atccttagag actatagctc aaacactaaa attgcaattt tcgcaccaat
      241 ttattcagta tccggagtta tttcaacctt tataatgaca accttcttta taaaaagctc
      301 cttgtacagt ttcggaggaa cagtagccat ggcaattggg gccgtgtcgg tatatgcagc
      361 cggagctgtt ggagcattct ttgccatgaa gctgtatcag acgaggctta gtaaatgctt
      421 aacaaactaa cggtggagta gcctgtggtg cctggcacct tgtcttatgg caccgttcca
      481 atcccatttt tattttccgt acgtaaaaaa tttattctag aatcgaacaa atgtttgaca
      541 aatgtttttc tttgtaatat aataatcaaa gaagactaag aaaaacttgg tgaaaatatg
      601 gaagacaatt tgtttgaaaa gttaaggatt ttgtctgatg cggcaaaata cgacgtatca
      661 tgtgtttcaa gcggtgtaga aagaaaaaac aatacagtgg gcggaatagg caatgcttct
      721 gccgcaggca tttgtcatgc ttggtcctcg gatggcagat gtatttcact tctaaaaatt
      781 ttacttacaa acgactgcat ttatgattgc aaatattgtt tgaacagggt gacaaatcca
      841 atcaaaagag caacttttac tccggaagag gttgcggagc tgaccattca attttataga
      901 agaaattata ttgaagggtt atttttaagt tcagccgttg aaaaaagtcc cgaccatacc
      961 atggagaata tttacagggt acttgagctt ttaagatata agtataattt ttggggctat
     1021 gttcatgtga aggtcattcc gggagcaaat cctctattag ttcaaaaaac aggtatgctg
     1081 gcagacagga tgagcgtaaa tatcgagctt cccacaagag aaagcttatc cttattggca
     1141 cctcaaaaaa agatggagaa tattcttaag ccaatgtctc tcatacataa tgaaatttct
     1201 cgtgctattg aagataagag tcattttaaa aatgctcaga agtttgtacc gggcggtcaa
     1261 tcgacacaga tgataatcgg agccacccct gacagcgata aaacaatcct aagcgcatca
     1321 caaaatttgt atgataaatt caggatgaag agagtgtttt tttctgcata cgttccggtt
     1381 atatcatcac cgaatttgcc tgccgtaagt accatgcctc cgttgttaag ggagcacagg
     1441 ttgtatcagg cagactggct catgcggttt tattattata aatctgagga attgtttacc
     1501 gaagttgagc aaaatcttga tttggaattt gacccgaaaa tgatgtatgc attgagaaat
     1561 atagacaaat ttccaataga aatcaacaat gcatcatacg aagagctttt aaggataccg
     1621 gggcttggtg taaccagtgc ccaaaggata ataagggaaa gaaaaaatgc ccaaatttcc
     1681 tatgattctc ttaaaaaaat gaggactgtt ataaagagag ctaaatattt tattaccgtt
     1741 aaaggtaaat actatggcaa tacaaagatt gacccggaag caataaaagc agaaataagg
     1801 agtaaggaaa ttcaaagaca aatgagtatc tttgaattgt tataatcatg gattatttat
     1861 acgacggtac atttgaagga ttgatgactt gcatttatta tcattacaaa aaagaaaagg
     1921 cgaccggtat atatgaaata tctgaatatc agcaatcaat tgtatatgag gcggaaattg
     1981 taaaaacaga tatttcgaaa gcaaaaatag tgtgcgattc aatcaatagg cagatttcta
     2041 aagaggctta tatacatgtt tattattgct atctttcaaa tgattctaaa aaggaaaact
     2101 tgattttaga ttttttaata tttgcattta agtacggcag agataccatg aacttctata
     2161 cacatgaaaa ggtccttcct attaatgaaa tatacaaaaa ggtagcaaga gaagagcaca
     2221 gggtgcttgg catactcagg ttttctgata taggaggaat tttgtttgct aaatattctc
     2281 ccgataatga cataagcatc ttattggctg accattttgc agatagatat aagcatgaaa
     2341 agtttattat atatgatgaa aaaagaaaaa aagcggttgt gtacgctaat aacagttggg
     2401 aaattaaaga aaacataaat attgataatg ttgaattttc tttagatgaa acaaagatac
     2461 ataaattgtg gaaacaatac tttaccgatt tggcaattaa ggagagaaaa aatattaact
     2521 tacagtttca atttgtaccc gcaagataca gaaaaaatat gactgaattc aaataaaaac
     2581 agcagacact tcaattagaa atgcctgctg ttattgattt agagtatatt tttctttgtc
     2641 attctgaacg cagtgaagaa tctatctttt aagacatggg atccttcgca tacgctcagg
     2701 ataagcaact gacaccaaat caaagatttg ggtcatttgc ttaatttgta aacatttgtg
     2761 tccaatatgg tctaccgtta ttcacatagc caacacctat ttttgtatag tgtgatccaa
     2821 gtatatttgc tctgtggccc ggtgaattca tccatgcact tactacttct tccggtgttc
     2881 tctggcctgc tgctatattt tcaccccatg cactgtatct gattccgtgc tgagaaagca
     2941 tatcaccggc actaccgtaa gttggagatt ggtgtgcaaa ataattgttg tctgacatat
     3001 cttttgattt gattcttgca acatttgata ttgcaggatc caaagtaagc ggtgctaatc
     3061 cgtttttctg tctttcaaca tttacaagct ctactactct tctttcataa tttgatactg
     3121 aaggagcagc agttgttgca gcttctgttg ttgctctttc atttgaagga gcttgagctg
     3181 tttcggctcc gttatttaca ggagcttgag ttgtttcggc tccgttattt acaggagctt
     3241 tttcaatatt tgtttttatt tcaatgttat tgtttgtatt aacaggttca aaataataat
     3301 aacctaaatt tttacacaag tcataaatat taaagtttgt ataatacact tgttctgcag
     3361 caaatgcaga tgttgaagat agtgctataa ttagcgctaa tgttaaagat aaaattttct
     3421 ttttcatttt ttgtttctcc ttctatatat attcttttac cccttacact tcagttgaag
     3481 cgtgtaactt atttttagtt actgacgaat tatagcacga tacttttcgt aatgaaaccc
     3541 tcaatattat gttttattgg taaatgtaac aaatatatta cacaattatg aagcaaaagt
     3601 catatattac aagtttatta caaaaattaa actaaattat tgcatgactg gtaattatta
     3661 taaacaatta ttgaatatga gactgcttcc cggggcgggt tcggagagag gatttatgtg
     3721 aaaggttaaa atatttgagc tatgttttat aatgtattta ttagataaaa tattatcaga
     3781 tgcaaggggt aagcagtgta aaaacttatc tgaagaattt tgttttttct tccttccttc
     3841 ttgttataga aatagattgg tattaatgca agcaaagaga attgttgcag cattcctgcc
     3901 gaaagattta ctgcaaacat aattaatcct atagtgagaa attttttgtg ctttgcctga
     3961 tttctagtaa aataaaatgc taaaatataa ataataccta taaattgata atccgttcta
     4021 agcaaggttg caatgacaga tgctgcaata ataataaaag cactattggt gctatatttt
     4081 ccttccttgt tatcaataaa atatatggca gctaaaccta taaagagagt aaaaaatata
     4141 ttttgatgct caaaaccgat tttaccgaaa aatgccaaat caaaaggtat ttccgaaatg
     4201 atagcaaatg ctatcagtct tgatgcatat ttctttatat tgctagtgtg ggtatatcct
     4261 tcaaccaata agaaacaata aatcgggaat gctaatcttc ctattattct gtaaataatt
     4321 atattacctt gaaaaattgc tccgtaatga tcgattatca ttgtgattag tgccgttatt
     4381 tttaaaacca gactgctcat gattttctcc atttttgtgt tttcaattga ttatatcaat
     4441 ttatatttat aatttcaaca aaaaaggcag ctactgctgc cttttgcgag gttccatata
     4501 gggggtttaa ttattgggtt ctttttttgg tgtaaaatat tatatttttg gaggagaaaa
     4561 ttctatgatg ctaacatttg tactattgaa gctttaggtc ttactccaat cattttgtta
     4621 acaactttcc cgtctttaat ttttaccaat gttggtatac tcataatgcc aaatgctgat
     4681 gctaattccg gctgctcatc tatattaacc ttgcctattt ttgcatcggt tacttctttt
     4741 gctatctcct ctattatcgg agaaatcatt ttgcagggac cgcaccaggc tgcccagaaa
     4801 tctaagagta ccggtttatc tgattttaaa acttcttgct cgaagttgtc gcttgtaatt
     4861 tttactgtat tcatatcata atctcctttc agcttattat atttttatat tacctgatta
     4921 aagtattatt gcagtgacta agtcacaaat cggtaatttc agttttatga tatgatttta
     4981 tccgatttaa atattattgc agtgactaaa tcacaaatca taatatagtt ttaagtctat
     5041 tcatttacca aaatcaaatt tattaaatta ttttaaaagg gtatactata aaaaaattat
     5101 tggagattaa aatggaaaaa ttatttttac agacagaaaa cggattaatt ccccttgacg
     5161 aaaaaatgat tgaaaagtat aacttagtga aaggcagaaa atctcctttt actcgttatt
     5221 atgtagttga cgaaacggga aatcacaatt taggttcaac aaaagaaaca tcaagtgacg
     5281 acgttgatag aatgcctgcc ggggaaggtg cagaagatga tgaaattgta gaattctccg
     5341 acacgggaac aatcctttca cagtcggaaa ttattgattt ttctcacgga acagattcag
     5401 aggaaccata gtgataaaac tattgacaat tgagcagaca ttcaactata atataatcaa
     5461 acggttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat aaaactattg
     5581 acaattgagc agacattcaa ctataatata atcaaacggt tgaacaatca ttcaactgaa
     5641 aacgggaggt agaaatgtct tctaatgaag gttttgtatg cgactgtgaa gtgattcatg
     5701 gagatattgt taatgaggtc aaaaaaagga tgcccgatga tgaggatatt tatgatttat
     5761 cggatttctt taaggtgttg ggtgacagca caagaatgag aataatttgg gcgctggatg
     5821 aaagcgaaat gtgtgtttgc gatattgctg ttttgttgaa tatgaccaag tcagccattt
     5881 ctcatcagct gcgtgcatta agaaatgcaa atttagttaa gttcagaaaa gaaggcaaga
     5941 cggtttatta ctccttgaag gacaagcata taagggatat atacgaaata ggtatggagc
     6001 atatcaaaga aaaatataac aaataaatca aagcaaaata taatgaataa actgaaccaa
     6061 tatgtggagg aagatatgac aaagacgtat aaattgcaag gattggattg cgctaattgt
     6121 ttagcaaaaa tggaaagaaa gataagcaaa cttaagggtg ttaaaagcgt tacatctaat
     6181 tttttcacag agaagatgat aattgaagca gatgatggtc ttatgcctga aatagaagaa
     6241 gctgcattaa aaataataaa aaaatatgaa ccgcaggtag tatttgaaga agcttaacag
     6301 gagaatttat gaaaaaatta ttatggcggt taattatcag tgcaatattt tttgtttctg
     6361 caataattat aaatgacagc caattaaaat taataatgta tataataagt tatatattag
     6421 ccggtggcga tgtagtaaaa agagcaattg aaaacattaa aaacggacaa gtttttgatg
     6481 aaaattttct gatgtctgtt gcttcaatcg gagcgttttt tataggtgaa gctcctgaag
     6541 gtgtcggcgt aatgttattt tatcagattg gtgaaatgct tcaaagctat gcggtgggac
     6601 aatccagaag atctatttca agtttgatgg atattcgtcc ggattatgca aatgtattaa
     6661 ggaatggcga agttcttaca atacctcctg aagaagtgga gataggtgaa ataatagtaa
     6721 taaaagcggg agaaagggtt ccccttgacg gtattgtcgt ggaagggaac tcaatgctcg
     6781 acacatcagc tcttacaggc gaatcacttc caagggaagt aattacggga agcgaattgt
     6841 taagcggttg cattaatatg aatggattgc tcaacgcaaa ggttacaaag agctatagcg
     6901 aatccaccgt aaataagata cttgaattgg tagaaaatgc aagcagcagg aaatccaaat
     6961 cggagaaatt cataacaaga tttgcaaggg tttacacgcc agttgtggtt gtaatagcag
     7021 ttgttctcgc aattataccg ccattgatta ttaagggtgc tacatttaat gagtggattt
     7081 acagatcatt ggcatttttg gttgtatcat gtccttgtgc attggtaatt tcagttcctc
     7141 tgagtttctt cggaggaata ggaggtgctt ccagaaacgg tattcttgtt aaaggaagca
     7201 attacctgga atccttggcg aaaaccgaaa taatcgtttt tgacaaaacc ggaacattaa
     7261 ctaagggtgt tttcaaagtt caaaaaattc attctgagaa aattaaaccg gaggagcttt
     7321 taaaacttgc agcttatgca gaaagttatt caaatcaccc gatttctctt tcattaaaag
     7381 aagcatacaa aaaagatatc gacattgaaa aaatttctca agtggaagaa atttcaggac
     7441 atggtgtatc agcagcagta gaaaatataa aagtcttggc tggaaatgat aagcttatgg
     7501 aaaaattcaa aattccatac aataaagaaa catctgaagg aactctagtc catattgctg
     7561 cagacggaga atatgcaggg tatattctga ttgctgataa gataaaggat gatgcttttg
     7621 aagctgtaag tgatttaagg aaacataaca tcaaggatat agtaatgctt accggtgaca
     7681 gtgaatcggt tgcaataaat actgcaaggg aattgggaat tgacagggtt tatgccgggt
     7741 tattgcctgc agacaaagtt gaaaaagtag aagaattgat ttctcaaaaa tcgaaaaagg
     7801 taacattaat gttcgtggga gacggtatta atgatgcacc tgttttggca agagccgatg
     7861 tgggagttgc aatgggagct cttggctcag atgctgcaat tgaagctgct gatgttgtaa
     7921 ttatgacgga tgagccgtct aaaattgcaa aagcaattga aattgcgaaa aatactataa
     7981 agattgcata tcaaaatatt atttttgcca tatcggtaaa agtgataatt ctggttttga
     8041 gcaccttagg ccttgcaagc ttatgggctg cagtatttgc agatgttggc gtaaccttta
     8101 tagctgtatt gaatgccatg agggcactta gacttaataa ttaaataaat atttaaaaaa
     8161 atgtgagggt tttagcccga agaatctcat gaaaaatctc aattacctgt tgagattttt
     8221 tgtttaatat gctaaattat ggatatatat aaaagtacaa taatataaac aactcatcta
     8281 tgcgaaacat tatacgacag ttctcaagaa tcgaaacatc tcggtgtttt ttctcaatct
     8341 gatacatttt ctcatcatga cataatttca ggcatgcatt ttatatcatt tgactgtcct
     8401 acaatccgtg tatgacaaag acctagaatc ttaaaagctt gtcataaaaa ttataatata
     8461 aaatattatt tatattaaag gggtggtgcc ttggaaaaat ttattgttga aatcggcatg
     8521 ggagcggatt tccatggcca ggatgtgacg gatgagtgta tccgtaaaaa ttaaaaatga
     8581 tgggaggtag tattttgaaa atcactaacg aacaattgaa agaaatgtat ttgaccatgc
     8641 taaagattcg aaagtttgaa aaaagtgcgt cagaattgtt tgctgcagga aaaattccgg
     8701 gctttgtcca cctctatata ggtgaagaag ctgttgcaac aggcgcttgt gcaaatttaa
     8761 gggaagatga ctacataacc agcacccaca gagggcacgg acacataatc gccaagggcg
     8821 gagaattaaa atacatgatg gctgaacttt ttggaaggga agacggatat tgcaaaggca
     8881 aaggcggctc aatgcatatt gcggatgcaa ccaagggcat tttgggagca aacggtattg
     8941 taggcgcagg tcataatatt gcagtaggtt cgggcttggc tattcaacac aagaaaacag
     9001 accaggtctg cgtgtgcttt ttcggtgatg cgtcaaccaa ccagagtaca ttccatgaag
     9061 ctttgaactt ggcaagtatt tggaaactcc cggtaatatt tgtgtgtgaa aacaacgggt
     9121 atggtatttc cgtaagccag aaaagacacc aagctattgc ggatgtttcc gtgagagcat
     9181 ctgcatataa catgcccggt attactgtag acggaaatga tttgtttgcg gtatatgagg
     9241 ctgtgagcga agcagttgca agagccagaa aaggacaagg tccaaccctc attgagtgca
     9301 aaacatacag gtggagaggt cattttgaag gagacccggt tgtgtacagg ccggaaggag
     9361 aattggaagc atggattaag aaagatccaa ttcccaaggt agaaaaatac ttgctggata
     9421 acggtgttat gaatgaagag gaaatgaaga aaatgaatga cgatgtggac aagatggttg
     9481 aagaggcaat agagtttgcc gaaaagagcc ccttcccatc attggaatca gctttaaagg
     9541 atgtgtattc tgatatagtt gaggagggaa gagtaagatg aaaaagatga catatgccga
     9601 aggaataagg gaagcaatgc gttttaaaat gcgggaagat cccaatgtta tactgtttgg
     9661 agaagatgtg ggtaaattcg gaggatgctt cggagtcagt gccggcttgt gggaagagtt
     9721 cggggatgac agaattcggg acacccccat atcggaaggt gtaatagtag gtgctgccgt
     9781 aggctcagcc gcatgcggaa taaaacccat cgctgaaata atgttcatag attttatcac
     9841 ggttggtatg gaccaattgg ttaaccaggc agccaaaatg aggtacatgt tcggcggaga
     9901 aataaacctc cccatggtta taagagtacc ggcaggagca ggcattggtg ctgcagccca
     9961 gcactcccag tccctggagg gatggctgac acatgtgccg ggccttaaag ttgtttatcc
    10021 gtcaaatgcc cgggacgcat acggcttgct gctatcatcc attgatgacc aaaatcctgt
    10081 ggtatttatt gaaaataaag tactctatgc aatgcaaaac gatgctgatg aaaatgaatt
    10141 aaagccaata cccttaggga aggcaagaat tgctaaagaa gggaccgatg ttacaatcat
    10201 cacatacggc cgacaggttt atgacgcctt ggaggcagcc gaaatcttgg ccaaggatgg
    10261 gataaatgct gaagttattg atttaagaac cctttatcct cttgacaaag aaactgtatt
    10321 caattcggtg gaaaaaaccc ataaggttgt tttggtaagc gaggagtgca agaggggggc
    10381 ctatacggga gaaattgcgg caatgatagc agaagagcgg ttctatgaat gtgatgctcc
    10441 catcatgagg gtgggtgcct tgaatacacc catacctttt gccaccgacc ttgagtctta
    10501 tgtgctacca aaagcaaggg atattgaatt ggctgtaaaa tcattgtttt aaactaggat
    10561 aaggccgggg ctgttgcact atgcggcagc ccctgctgtt aggaggttta tatggcttca
    10621 ataggaataa tcgcgaatcc tgcttccggt aaggatatca gaaggctggt atctcatgcc
    10681 acggttatcg acaacaatga aaaaatcaac atagtggaaa gaattgtact tggagcccag
    10741 gctttaggag ttgaaaaggt ttttgtaatg cccgactcat acaatatggg atacagggtt
    10801 gaagataagt taaacagctg taatgagctt agatgtgaaa ttaatgtggt taatatgatg
    10861 cggtttgacg gtatggagga caccgtcaag gctgccgatt atatggaaaa gaacgatgat
    10921 atcaagtgcg taatcatatt agggggagac ggcaccaaca gggcagcagc aaaaagttta
    10981 aaagacactc ccataataac tatttccacc ggaaccaaca acgtatatcc aaccatgctt
    11041 gaaggaaccg ttgccggcat ggcagctgcg gtggttgctt ccggtcgatt tgatgacata
    11101 agatatgtaa ccaaggacaa gagaattgaa atatacaaag atggagacct tattgacatc
    11161 gcccttatag atgccgtgat atcaaaggat gttttcatag gctcaaaggc aatatggaat
    11221 atagatacaa tcgaaaaaat cattgcaacc agatctcacc cggcttccat cggtttttcc
    11281 tccctggtgg ggtgcaaaaa aattatttat ccggaagatg atttcggagc ctatgtggat
    11341 atcaactcag gaagcgtaag gattaaggcc cccgttgcag caggagtggt ggaatccgta
    11401 tctgtaagcg agccggtaat tttgagatta gatgatgagt atgaattcac ggccaaggac
    11461 aggggcacca tagctttaga cggagaaagg gaaattgaat tcaaaaagga tcagaagttg
    11521 atttttaaaa ttacaagaga gggtccttat cacgttgatg ttataaaggc tttagaaact
    11581 gcacaagaaa ataatttttt cattataaaa aactgatact aaggaggtaa tatggctgaa
    11641 ttcattgtaa tgccaaaaat ggggcttacc atgacaaagg gcatgctgac aaattggaaa
    11701 aagaaagaag gggacttagt aaataaaggc gatatcctgt ttgaagtgga aacggataaa
    11761 ataacaaaca aagttgaagc caagatatcc ggaattttaa gaaaaatctt ggttgacaag
    11821 gaaaccgtag atgtttttgt acctgttgga attataggta gggcggatga agatatttct
    11881 gaattgttgg agcaggcatc aaaagagctt aaaacggata agccgcaaaa ggaagaaggt
    11941 cccaaagaaa caagggaact atcgccggct gtacaagcag gagatagagt aaagatttct
    12001 ccgagagcaa agaagatagc aaaggattta aaggtggatt ataatttggt gcagggaacg
    12061 ggaccgggcg gcgccattac ggaagaagac ataagaaaat ttgccgaagg tacaaaagca
    12121 aaggtatctc cgacagcggc tatagttgcc gaacaattgg gagtagacat cttcaatatt
    12181 gaaaaagaca ccaggataat gaaggcagat gtaattaaat ataaattgga tgaaagcttg
    12241 gcaaaatatg cagatcccca agaacaccgc caatccatga ccaatatgcg aaagattatt
    12301 gcaaagagaa tgcttcacag cgttcaaaca tctccatccg tatcatttaa cataaaagtt
    12361 gacacaaccg ggatgaaaga gctgagagat gaattaaagg atacattcaa ggtatcttac
    12421 acggatattc ttgtaaaaat agtatccgaa gtattgcttg agtttcctct tttaaacagc
    12481 acccttgacg gagatgaaat aataacccgg aattatgtaa acatgggtgt tgccgttgcc
    12541 cttccggcag gactcctggt tcctgtggta aaattcacaa acgtaaaagg cttaaaagaa
    12601 atttccaatg agattaaaga cctaagcgag agagcaaaaa ccaatgattt attgccggat
    12661 gaactgaccg gcggaacatt caccatcagt aatctgggaa tgtatggaat cgaatccttc
    12721 acccccatca taaatcagcc tgaagttgca atattgggag taaacgcaat tcataaggag
    12781 ccggtcgtag taaatgatga agttgttatt aagccaatga tgaatttaag ccttacggca
    12841 gaccacaggg tggttgacgg ggcggttgca gccgaattct tagcaaaatt aaaaaaatac
    12901 attgaaaatc cgtgcttgct tttattatag atactgcctt gagatgtctc aaatcccggt
    12961 tgaagtattt gcaaatgatg atataatata acaaataatg ttttgtacat aagcggacaa
    13021 acaacaatag gaggaaacaa tggaattaaa aaattaccag gatttgattg acagaattcg
    13081 caaaactgat tcgaaaaaac gtgtagccgt tgctgcagcc catgatgagc atacattgga
    13141 agctgttttc cgagcagctg atgacaaatt ggttgaacct gtattaatag gaaataaagc
    13201 aaaaatcata gaaatattaa aagagcttaa tgtagaatac gatttaaaca gcattattag
    13261 tacagatagc gacaaggaat ctgcagaaaa aaccgttgaa ttaataaatt caaacatggc
    13321 agacttcatt atgaaaggaa agcttcagac tgcagattta ttgagggcgg tagttgacaa
    13381 agaaaaaggc ttgagaacag gcagggtaat gtcccatgtt gcatttttgg aaattccggc
    13441 ataccataag ctgattgccg ttacagacgg cgggatgatg atgtatccaa atgcagagga
    13501 aaagaagcag atactcgaaa atgctgtcga tgttttccgt gccatgggat acgagtgtcc
    13561 caaggttgca gtccttgcag cggttgaaac cgtgaatcca aaaatgcctg aaacagttga
    13621 tgcggatatt cttaaaaaaa tgaatcaatc gggagaaata aaagattgtt tagttgaagg
    13681 acccatttcc gtggatttga ctttcaacag ggaatcagca gaaatcaaag gttttaaaag
    13741 tcctgttacc ggagaagcgg atatattatt ggttccgagc ataacgacag gaaatataat
    13801 gtgtaaagca ttgatagaaa tggcaggcgc aactatggca ggaatgatag tgggagcaaa
    13861 agtcccggta gtattggcat caagaggcgc atcatcacag gaaaaatatt tatcgatagt
    13921 tttgtctgct tcatcagtaa aataaatatt ttggaataaa atacggggac attcctttaa
    13981 cgcactctca aagattttcc tgcgttaaag gtgtgtcccc atacttctaa tgaatgggtc
    14041 ttgggcatct cgcattgaaa tttgtttagt ccatcttaac tgaatctgtt tgtagcagat
    14101 tttgatatat ttttaagtgc tatttcagcc tcatcctgca gtcttggttc aacagaaata
    14161 tcacctaatg atacgattcc tactaatctg ttattttcta cgacaggaag tcttctaatc
    14221 tggttctcgc tcattattct cacagcatcc tcaacgtcca tatcgggagt tcctaccaca
    14281 ggatttgcag tcattacttt ttttacctgc aaataaccat catcgcccac agctactgct
    14341 cttaaagcaa tgtccctatc ggtaattatg ccgattacgc tgttttgcgc acatatcgga
    14401 attgagccga cattgtaagt tttcataagc tgcgcagctt tttcaacagt atcttctgca
    14461 cttaaacatg caacttctct tgacataatg tctcttaatt tcaaaatatc acctccaaat
    14521 atatatttac ctttattctt tgttgtattc agatttatga ataaaaaatg aattttcggt
    14581 atttattgtt taaagcagtc cctgatgggt aaaagtattt atatattaaa aggagggtat
    14641 tttatatgag tattgtaaaa tggaacggaa gaaatttact taaatcaata gctgaaaatg
    14701 aaaaagaagc aaccaagtta tacagaatgt ttgcaagtga agcaagaata ggagagaaat
    14761 tttttgaact gttggcaaag gatgaagaaa gacacgaaaa aatctacaat gcactgcttg
    14821 aaaaatattc agataaactg gaactggaaa tggaagaatc cgatgcggaa tacatggatt
    14881 tattagttga aagcaatatc ggtttcgatg atgaactagt tgaaaaggca aaaaaaatat
    14941 ttactaaatc acaaatattt gatttggcag agagagctga gagagatgca gtattattcg
    15001 ttactgaatt gcaaagattg tatcccgatt tggcaaagga tgaaatggca attatcttaa
    15061 aggaagaaaa atctcacttg aagaaagttc tggaaagaaa aaaagaaagc cagccaatgt
    15121 ttggaagagg catgtaaaat attatcttct ttaaaaaaat aatgcattat tattccattt
    15181 gtattataat agtgtcacaa aatcaatttg acacaaatga aaaatgcaaa taaaggagtt
    15241 aataaaatgg cagataaaaa cattaagaaa gaaaacaaga aaaaaaagaa aaaagaaaca
    15301 aaacaagcaa atgtatcatc agtatcttat gaacaacctg ttatgagtca gcctgaatta
    15361 gttcctaaaa agaagaaaaa agatgaatag tatctaaaaa tgtgtctcgg atataccgga
    15421 cacattttct ttattgcagt aattgattgt tttttttata tgttgtataa ttaaataagt
    15481 tttttgtcac aagagagagt tcgaataatc tcaacatatt gttttaacag caaaggataa
    15541 agggatatat catggtctcg gtaaatcagg agggaatatg aatattttga tattaaactg
    15601 cggcagctca tcaatcaaat ttcagatagc agaaatgcct gattacaatg taattataca
    15661 gggcagcgta gaaagaatag gatatgaaga tgcagaattt aaatttttct tagtaaagca
    15721 aaaaataatt cagcctgtgc taagtcacga agaaggcatt aatttaatca ttaatactct
    15781 tttcaaaagt aattttggaa ttgaaacaaa agacattaat gcaatcggtc atagagttgt
    15841 gcagggagga gataagcaga atatttcggt ccttattgat gataaagtta tagactatat
    15901 agaaagtata aaggacttgg cccctcttca taaccctaac catttggcag gaatttatgc
    15961 atgcaggaag cttcttccca atgtaccgca ggtggctgta tttgataacg gatttcatat
    16021 gtcgcttcca aggtctacat atatctatgg tattccttat gaatattatg aaaaatatcg
    16081 tatcagaaga tacggctttc atggtatcgc attcagaagc atggcggaag aagttgataa
    16141 aatattaaat gagactcata agaattataa aatagtaaat ttaatgcttg gaagcggaac
    16201 aacggctaat gctttaaaat acggcaaatc agttgaagtg agcacaggtt tcactcctca
    16261 cgaaggactg atacagtcta caagagccgg agatattgat gctgctgcca ttttatattt
    16321 aatgaaaaaa gaaaatttat ctattgaaca agcggatgaa ttaataaaca aaaaaagcgg
    16381 atggaaaggt ctttccggta tcagtaacga tatgcgtgaa atctaccaag catcacaaag
    16441 cggaaacgaa agagcgaaac tcacaattga tgctgtatgc cacaggttta aaaagtatgt
    16501 gggtgcttac agtgccataa tggaaggtat tgatattctt gtgttttccg gtggagtagg
    16561 tcagaatgcc ccttatataa gagaaaaagt atgcaaggga ttggaataca tgggaattga
    16621 gctggacgag tataagaatc aaaacactgc aggtataatc agcaaagatt catcaaaagt
    16681 aaaagttatt gtagttaatg caaatgaaga aaaaataatt gcccaagata catatgaaat
    16741 atgcaagtaa gtctgagcga taggaaagga ttatccataa acaatcggaa tactcattgc
    16801 tatagtattt tatgcattat tgggtagaat taataaaaat atatactaat gctgttataa
    16861 gctgtcaact aataactaag tatagagagg tagaatttga ataaaaattg ttttgtttca
    16921 tggagcgggg gcaaggattc ctgtcttgca ttatataagg caatagagat gggatataag
    16981 cccgtcaagc tttttaccat gtttggcacg gaaaaaatca gttcctctca cagattggat
    17041 gagaatatta taaaattaca ggcggattca atagggattg aatacctgat agggaaatca
    17101 gactttgatg aatatgagaa ggtatttgtt tcaaatctaa aaagttttaa agaccaggga
    17161 attgactatg gaatattcgg agacatagat ttagatgaac atagacagtg ggaagaaaga
    17221 gtatgcaaat cagcaggtat ggaagctatc ctgccccttt ggaagaggga tagaaaagaa
    17281 atagcaaaag aatttatttc gcttggattt aaggctaaaa tagttgttat caacaccagt
    17341 atgatagata taaaattttt gggaaaagat ttaagtctgt ctttgataga agaaattgaa
    17401 aaggcaggag ctgacccatg cggcgaaaat ggagaatatc atacccttgt gtatgatggt
    17461 cccattttta aaaaaccggt ggatataaaa tatgattatg aaattattcc cgtaggagaa
    17521 acatgggccc agattaaagt atattaaagg gatacaatta ttgttgaata aacattattt
    17581 tataaactat actaataata taaaggggtg atatcgtgga cagatttatt attgaatttg
    17641 gtatgggagt ggatttccat gggcaagatg taacgaaagc tgcagtaaaa gcagcaaaag
    17701 atgccatatc caaaagttgc cttataggtc ttaatgaaat atgcggattt aaccaggata
    17761 atgttgatga caaggtgctg attgatgtta cgattggagt tactcgtcct gacgaggtga
    17821 agattgacga ggttgtaaag tgttttcctg taggaaaggt aaccgtaaaa gcggtttatg
    17881 gcggtttaaa aaccgaaggt ctttattttt caaaattcgg agataaggat aattcaattg
    17941 aagttgcagt tgcaagtgta tctgttaaaa ttaaaaagta aggggaggaa taatgaaaat
    18001 agcaataata ggcggaggcc ccggcggcta tgtagcagct attagggcgg ctcagcttaa
    18061 tgcggaagta acgctaattg aaaaagagca tatcgggggt acatgcctca ataaaggatg
    18121 tatacctact aaggttctgc ttcaaacttc aggagagttt gaagcagtca ataaaagttt
    18181 taaggacttg ggcataaaaa tatccggagc agaattagat tggaaacagt tacaaaaaag
    18241 aaaaactaaa ataataagaa aattagtagc cggagtggat aacctgttaa aaaacaataa
    18301 agtaacaaaa ataatgggag aaggaagctt tgtcaataaa aatcaattaa aagtaaagac
    18361 tgcagacggt gctgaaatat ttgttgattt tgactatgca ataattgcga caggttcaaa
    18421 acctgtcgta attcctatac cgggggttga actgccggga gttgttacaa gcgatgaagt
    18481 attgtctctt gaagaaatcc caaactccat ggttattata ggaggaggag taataggttc
    18541 tgaatttgca gaagtattca gtgcagtggg atgtaaagtt acgataattg aaatgctgcc
    18601 taatattata gcaaatatgg atagggatat tgttgaaacc ttagtagaaa agtttaaaaa
    18661 gaaaggtgta gaaatttata caaataccaa ggtggtttca atttcaaaag cagaaaaagg
    18721 actaagcgta aatacactat cgagcgggaa tgagatatca ttcaatgctg agaaggtgtt
    18781 gctttcaata ggcagaaaac ctgttaccga taatcttgca ttggaaaatg tcggcgttga
    18841 aacaaacaga ggcgcagttc ttgtaaacaa aaatatgcag acaaatgtac caaacattta
    18901 cgccatcgga gatgctacag caggagttat gcttgctcat gtagcttcgg cagaaggaat
    18961 agttgcggtt gaatctatta tgaacaaaaa gtctaatata gattttaaaa caattcctta
    19021 ctgcgtttat acgaaacctg aacttgcagg agtaggacta actgaagagc aggcaagaga
    19081 aaaaggatat gatgtaaaaa tcggtatatt tccaatgtac ataaacggaa aggcaatgat
    19141 tgaaggagag caagaaggat tggtaaaata tgtagttgac gcagcaacgg gagaagtttt
    19201 gggacttcac atgtcaggac cgaatgcaac cgagcttata gttgaaggag ctcttgcagt
    19261 taggcttgaa gcaacggtgg atgaaattat atcaaccatt catgctcacc ctactgtagc
    19321 agaatctctc caagaagcag ctcatgcagt acataacaat gcaatacaca taccaaaata
    19381 aatttagaat ataatatgtt tagcctcata aattgtggat ataaaattaa tataacgtat
    19441 aaggagtgat atttatgaaa ctgcttgttc cggtagatgg ttcaaacgct tcaataagtg
    19501 cagtaaaaaa agctttggag ttggcaaaaa aatataactt ttcaataaaa ctaatttctg
    19561 ttgtttctgc aaagagttct gaatacagaa ggcatgaaaa tgcatggaaa ggaattgaca
    19621 gctccgtaat ttcggaaagt gaagagctgg aaagacaatt ggaaatgaaa atcaaagaaa
    19681 atgcacaaag attgttaaat gcaataatat ccaagttgga tttcagtggt ataagagtag
    19741 aattagaagt tcttttggga gaaccctatg taaaaatatt agaaacagca agaaatgata
    19801 atatcgatat gatagtaatg agtaacagag gtttctcgaa aataaaaaga ttttttgtcg
    19861 gctctgttac tcaaaaggtt atttcagaag caccgtgccc tgttttagtt gtgaacaccg
    19921 gttttgagcc ttaatgggaa attataaaat tagcggaggg aatatgcgtg ttgttataat
    19981 tggagccgtt gcggcaggaa cttcggcagc aaccgaaatc agaagaaatg ataaggaagc
    20041 agaaataatc atttatgaaa aggacaaata tatttcatat gcaggttgcg gtatgcctta
    20101 ttatataagc aatgaagctg aaggtttttc tagcgttgtt ccaagagaca aggatttttt
    20161 taaagaaaaa cataatatcg atatttatac tgagcatgaa gtagtgtctg taaatccgaa
    20221 taacaaaaca ttaagagtaa agaataaatc aggcgagatt atggacagat atgacaagct
    20281 gataattgcc accggagcaa tgtctgttaa gcctgattta aaaggctctg atagagacaa
    20341 tgtatttctt ttaagaaata taaatgacat gattgatatt aagaatttca tagatgaaaa
    20401 taaaccaaaa tcagctgtta ttatcggaag cggattcgta ggtctcgaaa tgtgcgaaag
    20461 cttcaaacta ttggggttgg acgttacgat tattgccagg tctaaaattt caaagggaat
    20521 cgacaatgac ttatctttat atattgaaga acatttaaaa gaaaagggag ttaatgttta
    20581 tacaaataca aagacagttg aaattaatga cagcggggtt gtattaggtg acggaagcat
    20641 tgtgaaagcg gacatggtac ttcttgctac aggtgctaaa ccaaatgtaa cccttgcaaa
    20701 agaaatggga gttgaacttg gagcaaccgg tgccatcaag gtcgataaat atatgaaaac
    20761 aaatattgag gatgtttaca gctgcggcga ttgtatcgaa atgtattatt cttttaacgg
    20821 caatcctgtt tacagaccct taggctctac tgcaaacaag accggtacga ttgccggaag
    20881 caatattacg ggaatagaac aagaatttaa aggaatactt ggcactgcca tatacagagt
    20941 tttcgaactt accgtgggga tgacaggttt atctgaggaa gaggctgtaa aatcaggata
    21001 taatgttgtt ataacgattg acaaaaggcc taataaacct gagtatatgg gaggaagacc
    21061 aattattatc aagactgttg ccgaaaaggg aacacgcaga ttattaggtg cccaattaat
    21121 cggttacgaa ggagttgaca gaaggcttga tgtgttggta actgctatta cttttaatgc
    21181 aaaggcagag gacttaatgc atcttgattt agcatacgct cctccttatt caacacctag
    21241 ggataatcta tattacacgg gagcaaaact aaaagctaaa ttatcagccc tttaaaaggg
    21301 ggctgatttt ttgggcaaag aatactatga ttacagcttt cagcatatct cccggtataa
    21361 atgcaaacat gcctaattga agaattttaa ctatacttaa attgctgtta agcattatat
    21421 ttaaaatata atacatatag ggaagtccaa aagtatatat aattaaggtt cccgcaaaaa
    21481 ctgccgccca taaatattga gaggcagttt tttcttccgt taatttacct acaacatagg
    21541 ctgcagcaac atatcctatt ataaaaccaa agcttggttt aaatatatgc tgcaatcctc
    21601 ctgaaaatcc ggagaaaatc gggaagccta taagtcccaa caacaaatat gttatttgag
    21661 aaaacatagc tttcttagaa cctaaaataa ttcctgacaa tagtatgaaa aatgattgca
    21721 gtgttatagg cactggacct atgggaatgc tgataaatgc accaactgcc gtcaatgcgg
    21781 tgaaaattcc tatttgtgtt atatctctta cgctgaatgt ttgttcctta tacatgtttt
    21841 tcctctcatt ctgtttttat taagtcttta tcatgagatc cttcgctatc gctcaagatg
    21901 actcgccagg gtttgtcact tctttttaat tgaaacttct cctgaattca atgttatttt
    21961 ttgtccatct tcggtataaa caaccaatcc tccataatcg tcgatgtccg acgcataacc
    22021 ttcatgccat ttattgtcct ttttatataa aatgttttct cccaatacca tggagcgctg
    22081 tttatatgat ttcataaaac tcttgtctgc caagttattt gctgctttca gcagattatt
    22141 aattatttct gctgcaagct gattccttat ataattgtca tcatcaataa atattgaacc
    22201 tgcagtattt tgtaattcca agggaaattc ttcatttttt gtttttacgt taattcctat
    22261 tccaactacc acgcaatcca tcatgccgct ttcaaagttg gtaaccgctt ccgtaagtat
    22321 gccgcatacc tttttatcac ctataaatat gtcatttacc cacttgattt ttgggtaaac
    22381 acttgtaaat ttttcaatgg cttgacagac agccacagct gctgcagttg tcactaagac
    22441 tgaattttct atgcttaatt cagccttaag aattatactc atataaatac ctgattcggc
    22501 aggtgagaaa aaatctcttc caaaccggcc tcttccctta gtttgttctt ctgcaatgat
    22561 tgtcgttcca tgctctgcat tttgaacagc taaaagctta gcctttgcgt ttgttgaatc
    22621 tattgtttta tatacggtga taggaatatt tttaaattct tcatttaaat acaatcttat
    22681 tccttcggca gataacatat cggacgattc agacagagag taccctttgt tgggtgttga
    22741 tttaatgtta tatccatcat cttttaatga attgattgct ttccaaactg ctgttctcga
    22801 aacattcagc atatctgcta attcttgacc tgaaagagtt ttacccttat tggactctaa
    22861 aatatgtaat atattatttt ttactgacat aatttcacct caataaacaa tattataaaa
    22921 agcaattgta agattgtcaa ctaaaaaata attatggttg accttgtatg caatttatat
    22981 ttgttatata gagaaattct attgatacta tataaaacta gtattggaca tatagatatt
    23041 attaatataa aataatatta ataacattgc gtgcataaat aatattgaaa ggagtggcat
    23101 tttgatactt ccatcagaaa taagattatg cgaggtaggt ttgagagatg ggttgcaaaa
    23161 cgaacctaaa attttaacca cagaagaaaa gctcaagctt gctgaaggtc taattaatgc
    23221 aggttttaag gtaatagaac taggttcttt tatgagccct aaagcagttc cccagatggc
    23281 agacacggac gagctgttca ggcactttaa taatgttgaa ggcgtggaat tgcgtgcgct
    23341 tgtagccaat aaaaagggag ttgaaagggc gataaattgc ggttgcggta aaattaagct
    23401 gaatgtttct gccagcaaaa aacataacct tgcaaatata aataaaacac ctgaggaaac
    23461 tgtggcaggt tttgcggaaa atgttgacct tgctttggaa aacggccttg aagtttcagg
    23521 ctccatatcc atgccattcg gttctccttg ggagggcgta atccctatta aggatgtaga
    23581 agaaattgtt gaagcttatt taagagtagg aataaaagaa atttcccttt cagatgctgc
    23641 aggtgtggct tcaccaaggc aggtttatga catatgctct tcaatgcaaa aaaaatatcc
    23701 ggatgtaaaa tggtggctgc actttcacaa taccagagga atggctatgg ccaatatttt
    23761 agcttcaatg gaagcaggaa taacaaactt tgattcatcc ttaggaggct ttggagggtg
    23821 tccttttgtt cccggagcag caggtaacgt atctacggaa gatgttgttc acatgatgga
    23881 cgaaatgggt ataaaaacag gaattgatat attgaagctt attgagcttt cgagaaacct
    23941 gcaggatatg cttggaaacg agggagactc atatattcta aaagccggca gaactaagga
    24001 tttatttaaa tagaattata tattaatcta aattgtttaa actattctaa aatatagttt
    24061 tctatataat ataggaggaa atatgagcag aatgagttca atggacgagg cttatgcaaa
    24121 acttactcaa gaccaaattg acgaaataga agaaaagttt gcaaaagctg atgccgcaca
    24181 aaaaattttt gaaaaatgga gtcaagagga tattgataga actattaggg ctattgcatg
    24241 gaaagtagcc aatacacaaa cttttaaaga attagtcaaa atgagcatcg aagaaagtaa
    24301 attgggagac cccgtaagca gagagaataa aaaatataaa ataaggggag tacttcgtga
    24361 tgctctcaga caaaaaagcg taggaataat tgaggaaatt cccgaaaaag gaattgtaaa
    24421 atatgcaaaa ccggttgggg ttatagcatg tgttatacct gcaactaacc cggatttaac
    24481 acctgcaggg aatgcaatat atgcccttaa ggctagaaat gcaataatat tttctcccca
    24541 tcccagagca gcaaagaccg ggggcagaac gattgaactg atgagagagg gtttaaggca
    24601 ggtaggagct cccgaggatt tgatacaaat cctgggacag ggaaaagcta agataaacag
    24661 agccatgtcg gaagctttaa atacaaaatg tgatttggta attgcaaccg gcggtcaagg
    24721 tgtagtaaga agggcatatc actccggcac accggcttat ggagtaggtg caggaaatgc
    24781 tacaatgata tgggatgaaa cagccaagga tgaattatat aaagcagcat ataataccat
    24841 gctttccaaa acatccgatt ttgggtcggg atgttctgca gacggcaata ttgttatgca
    24901 tgagagcatt tttgaagaag gaataaatgc attaatagaa gcaggcgggt atttattgaa
    24961 tgaaagcgag caagaaagca ttaaaaaaat aatgtgggat gaagaatgcc accgcttgcc
    25021 tgattcggtc gcccaatcgc ctcagagaat ggcacagctt gcaggatttg aaatacccga
    25081 agacagaaaa ttcctgatag caatgggaac cggaagctat acaactgctt acgaggatgt
    25141 gtggtgccgt gaaaaattaa caacactttt ggctgttcat aaatatgaag gtgaatttca
    25201 aaatgcgatt gatataatca aagccattca cgaagtaggg ggtaagggtc acagcgtggg
    25261 aatatattca tacgatgaag atcatatcca tagacttgct cttgaagccc aagtaggcag
    25321 gataatggtt agacaacctc aatcgaaatc aaatgccggc tccttcacaa acggaatgcc
    25381 catgacatca agcataggat gcggcacatg gggcggtaat gcaacatcgg aaaatgttca
    25441 tttaaaacat tatatgaatg ttacctgggt ttctaaagaa atacctgagg acagaccgac
    25501 tgatgaagaa ttgttcggag aattttatga gcctgaaaga gaaggaaaat aattaaggag
    25561 gtaatatatg aaaagattag ttgcagacca aatggtagaa tatttggaaa gacgtaatgt
    25621 agagtacatt tttggtttat gcggtcatac ggtaattggt tttttggacg cattatcgag
    25681 aagcgaaaaa ctaaagtatg tatcatttag gaatgagcag cttgctgcta tggctgccga
    25741 cggttatgca agaatcaaaa agaaggccgg agtagtacta tgccatttag gcccggggtt
    25801 ggctaatacc gtaaccggag ttgccaatgc ttcgtttgat tcaataccca tggttgttat
    25861 tgcaggagat gttccaagat attattatgg aaaacatcct catcaagaag ttaatatgca
    25921 tgcagatgct tctcaatacg aaatactaag accaattgta aaaagagctt ggagaattga
    25981 cgatgcggaa gcactgcctg aaattttgga taaggctttc aggcttgcag aaagcgggag
    26041 accgggaccg gtgcttatat caatccccat ggatgtattt tctgttaaaa tagatgaaga
    26101 attatttaac cgtacattta aagacagctc ggaaacaata aaaccggcac tggcaccttc
    26161 agcagcaaag gcaatagcta aaaaacttat agaagccaaa aatcctgtta ttcatgcagg
    26221 aggaggaata cttttggcag gtgcatcaga ggaattaaga cagctggtag aatttttgga
    26281 cattcctgtt tcaagaacac ttatggggca aggatgtttg tcggataccc atcccttgat
    26341 gcttggtcaa acaggattct ggggtcttga gtttacacac aaatttactg caaatgcaga
    26401 tgtaatactt ggattaggaa cacgattcgc cgaagcagac agttcaagct ggtatacggg
    26461 tgttactttt gatatgaata aaacagaatt tttgcagata gatattgacc cggcagaaat
    26521 aggaagaaat tatcccgtat caatcggtgc aatagcggat ttaaaacttg ccttggctca
    26581 aattcttgaa gaagccaaag cccttgtacc ggaagggatt aaaagaccgg gtttaaggga
    26641 tatgattaaa gcaaatcgtg atgagtttag aaagtcaaat attgaagttt caacagatgg
    26701 cagattccca atgacacctc aaagaatttt aagagatgtt aaatcagtta taccggaaga
    26761 tgcggttatc tttacggatg taggctggaa taaaaacgga gttgctcagc aatttgatat
    26821 tactataccg ggaacaatcc accacagttc agggttggca actatgggat ttggcggctc
    26881 ggcgcttata ggcggcaagc ttgcagcacc ggataaggtt gtattaacac ttgtaggaga
    26941 cgggggattc ggagttaatc ccggagcatt ggcaactgca gtagagcaaa acattccggt
    27001 agtgtgggta gtaatgaaca attctgcatt tggaacaatt gcgggattag aatattcaaa
    27061 ttatcaaaca aaattcggaa cagtattcac aacacctgac ggtaaacctt attcgccaaa
    27121 ttgggcagag gttgccaaag cttatggagt tgatgctgta aaaataaaat cagctgatga
    27181 tttcaaaccg gaacttgaga aggcaatcaa gagcaacaag ccatatttgt tggatgtacc
    27241 gatggaaaat attgcagtgc ccacaccggg aagctggaat ataaatgata tttataatcc
    27301 taaggaaggt gttgttaacg gtaaattggt aaagaatgaa aaaggggaat atatgcctcc
    27361 agatcattcg acatcacata aataagtgtt accaaaggag gataatatga aaagaaagtt
    27421 ttcgctggct tatttgacca taccgggtac tcatccggtt gatcagataa gaattgccgc
    27481 tgaggcagga tatgattttg tgagtttaag accaattcct atgcatcttc caaatgagcc
    27541 attgtttcaa tttgatttgg acaggaaatt gttcaaggat ataagagatg cattggagca
    27601 aaatgatata aagcttatgg atattgagct cgcgcgagtt agagaagatt taaaagttga
    27661 agattatgaa tcagcatttg ctgcagcagc cgagctaggt gcaacagatg tgctctcaag
    27721 tatctggact aaggataagg aattcgcttt taaagaattt gcaaaaattt gcgatttggc
    27781 tgctaaatat aatttaaaag ttaatcttga atttgtaaca ttctccggag taaccgatct
    27841 tacgggggct ttagaagttc ttgacaaagt aggtagacct aatgcatact taatggtaga
    27901 cactctgcat gctcataggt ctagggttag tcctgaggat ttagctaaag ttgacagaaa
    27961 gaagtttgga tttattcatc tttgtgacgg tcccggtgaa attccgtcct tagaagaccc
    28021 ttccatgatt ggtgttgcca gagaaggaag actatatgcg ggagaaggag aaattgacct
    28081 taagggtatg cttttagcca tgcccgataa tccgatatca atagaactgc caaattctaa
    28141 ggaaatgaaa gaaagaggag cagcagggca tgctacaagg tgtctcatta cggcaaaaga
    28201 attattagtt aatatggcta aagaggagga tatagaatgc cagtcaatat aaaaacgaaa
    28261 atggttacat tgctgggaaa acctttatcc cagtcctatg ctgcaaggat gcaaaatgca
    28321 gcctatagag ctgccggaat tgatatgttg tatttctata ccgaagtaga aaatgaccac
    28381 ctgcctgatg tggtaaatgg aataaggtat atgaattttg caggttttgc ggttacaaaa
    28441 ccaaacaagg ttgaaattat gaaatatgtg gatgaaaagg atcccctttg tgaaaagatg
    28501 aatgctagca atacggttgt tatattacct gatggcaggc taaaagcata caatacagat
    28561 gggataggtt ttttcagagc cttaaaagaa gaatatccga atacaaaaat tgaagaatcg
    28621 acatttttct gtataggtgc aggcggcgcg ggaagagcaa tatgcagtgt gttggcttat
    28681 tacggtgcca aaaaaatata tatcgcaaac agaactttat caaaagctca gacattggtt
    28741 gatgatataa atcgaaattt tgcacctgtc gctgagttgg ttgatcttaa tgacacaaat
    28801 cttctaaaag aaaagatcaa agagtcggat gttgttatga ataatacagg acttggaatg
    28861 ccttcaagcc ttgatggaac acctatacct aaggaatatc ttcattccgg acaactatgt
    28921 tttgatgcaa cctataatcc gtcaaaaact cgttttctta ttgaggctga agaaatggga
    28981 tgcagcataa tgaatggttt gggaatgtct ttataccaag gggctgagca gattgaactt
    29041 tggagcggaa agaccgcccc aattgaagca atgcgtcagg agcttatgaa tatccttgca
    29101 gggaaaaagg aagagtaatg caaatttcca acaaaaggag gaaatataat gtctgcaata
    29161 aaatggttgg atgagcactt agaagaagcc attatggtga ttatgttagc acttatatca
    29221 tgcgtaatga tggctcaaat aattgctcga aatgttttta actcaatgac ctggcctgag
    29281 gaattttcaa gatattgcta tatttggact gtctttttat cccttgggta tacgataaaa
    29341 aaaggcaata tgttgaaggt tggacttgta atggatttat tgcccacaaa aataagaagg
    29401 tctatagaaa ttgtggctaa cttaataata ttagcaatat gtgtggtttt tttcagacat
    29461 gctatcacct ataccggtaa aatctacaat atgggacaga tttcgccggc aatgcattta
    29521 cccatgtgga taatgtatct ggcaactgtt acaggttttg gattggcttc aattagagct
    29581 gctgaggaaa tagtaaagaa cataaagaat tttaacgtaa aagttgaaac aacaattgaa
    29641 gctactttaa aagaggctat ggaggaagta gagactaccg gtattaacgt tgatgatgaa
    29701 cttaaggatt cagggggtga cgagtaatgg ctttatggat attcgtagta tttatggttg
    29761 cattgatatt tgctgtacct attgccatga gcatggtcct gggtgctttg actccgcttt
    29821 ttctcggcgg tacaggcagc tcaattcagc aactgatagc taacagcttc tcaggctcgg
    29881 attctacccc tatattggca gtacccctgt ttattctggc gggagtactt atggctgaag
    29941 gaggcatatc cagaaaactc tttaatttct ttgcttattt tataggtaat tttactggag
    30001 gacttccttg cgcagtaata ataacttgtt tgttctatgg agcaatttcc ggttcaggac
    30061 ctgcaactac agctgcagta ggctccatga ccataccctt cttggttgaa atgggttacg
    30121 ataaggtttg ggcagccgga atgattgcca catccggtgg tcttggcgta attataccgc
    30181 cttctatccc ctttgttctt tactcattgg caaccggtgt ttctacgggg gcacttttct
    30241 tgggaggtgt attgccgggc atagcaattg gcttaggcat gatggtttat gccgtatatt
    30301 attgtaagaa aaaaggagaa gacaaagaga aaatcaatgc aaaaatgaag gagcttagag
    30361 gcaaaggatt ttttaacctg tttaaagaaa gtttctgggc attactttca ccggttattg
    30421 tactgggtgg tatttattca ggttatgtaa cacctacgga agctgcttgt atttcagtgt
    30481 tttattcttt gtttgtttca ttgattgtat ataagtcatt tacggttaaa gaaataattc
    30541 cttttttggc aaaagcagta aaaacatatg cacctctttg ctttgtactc gcgtttgcaa
    30601 ttgcatttgg aagagtcttg gcattagcta aggcaccggc tattattgaa ggattcattt
    30661 tagacaattt tacaacaagt attaccgtat tgacgggtat agttatagta tttttaatac
    30721 ttggaatggt tatggatacg ggacctgcca tagtaatatt atcacctata ttactgccgg
    30781 cagttcaagc tttgggagtg catcccgtac actttggtgt tataatggtt tgctgcttat
    30841 cgataggttt ggcaacacca ccatttggat tggatttatt cgtggcgggg actttatcag
    30901 atacccctcc aatgaccgtt gcaaaaaaag ctataccttt tataattgca ttcggtatag
    30961 tgttgtttgt tataacatat gtaccatggc tcagtcttgc actcctgtaa aggatcaagt
    31021 atatatttaa aaagaaagga aggagaaaaa tgaaaaaatt attgatatta ttgttgatag
    31081 caacaatggc gttgctgccg gtagcttgta ctcaaccgag tacacctact ccccccgaac
    31141 aacctgaaca gcctgaacaa cctgaacaac ctgaacaacc tgaacaacct ggaggctttg
    31201 atgcacttga acctgtagtc ttgataggtg ctgataatgc aggagtcggt gctgcagctc
    31261 agctctatgg agaacttatt accgagaagg ttaaagaaat aaccggaggt aagcttacaa
    31321 ttgattattt cccaaacagc cagcttggaa atgaccagga actccaaggt cagatgcttg
    31381 ccggagatat tgacttcgta atttgtcaaa cagcgcaaac cgtatctttc gttcctgaag
    31441 tagcgatttt tgaccttccg atggtatttg caaaatacga cgctgaaacc attgattatg
    31501 cattaaacaa aagcgagttt acagataaaa taaaccaagc ttacaaggca aaaaatatgt
    31561 actgtctgca cttcctccag ggaggaacat tcagagaaac aacatctaac aagaaaattg
    31621 agacaattga tgatttcaac ggcttaaaga taagaacaat ggaaaacaag aaccacatgg
    31681 cattttggca ggcaatgggt gctgcaccta caccgcttcc atggccggag gtatatattt
    31741 cattgcagca gggattggtt gatgcacagg aaaatgccac agacacttgt gtgggagcaa
    31801 acctccagga agtacaggat tatctgattc tgacccacca tatactgtat tgtaatcagt
    31861 tcttaatcaa tgccagcaaa tttgacagcc ttgacccctt gtaccaagaa gcactgcaac
    31921 aagctattga tgaagctgct gtagaaattg aagctcaatt aaccgaaata gacgtaacaa
    31981 acagacaaac attagtagac ggcggtatgg aattagtaga atttgatgca agtttcatag
    32041 accaagttct agaaaaggca aagggtgtat atgaatctat aggcaatgat atcggacagg
    32101 aatatgtgga ttctttgcta aatgcacttg aaaaataaaa aacttatctg actaagcaaa
    32161 taaactgaac aggtagatta agaccgccgg caacagccgg cggttttagt accctctatt
    32221 cactacaaaa atttaatttt attgaatgaa tataactttg ataaaatagg aatcttcttc
    32281 atttagacag tgaaattttc aataataaaa tctcttgttt taattgcggc aggtgataaa
    32341 tatttatcat caatccagca caggtagatt tctcttttta agggcatatt acaaatcttt
    32401 accgtgttta catgtttgtt catggatggt atttccggta caatcgaaat tccgaaatta
    32461 agagaaacaa atgaaatgac agcattgcat tcttcagctt caaaaactat tttaggagaa
    32521 aaattatacc gtgcaaatat ttcatcaaca gtgctcctaa gagagctgct tttatttaaa
    32581 gcaacaaacg gttcgtcgat tatatcagaa atttcaatgc attttctttg agaaaaggga
    32641 tgctttttcg gcacaatcaa atgaagctgt tgactgaaga ttttcacgca tttaaggctt
    32701 ttatcaggtg tcgggcaaaa tgccagttgg attttattgc tttttaagtc ccttataagc
    32761 tgttcaccca aattctggac caatgaaaac tttattaagc ggtttgaatt gttttcatag
    32821 aattttttaa tcatctcagg gataaatttt gaacttatac tataaatata tccaagagac
    32881 acctctccca ttgaagaatc ggcaacggtt tttagcatta tagtgccttc atccaatata
    32941 tccaatgatt tctcaacata tttaagaaac agctcaccgg gattacttag ttttatcttt
    33001 ttcccatgtc tttcaaacaa cttaatattt aattcgcttt ctaattgaga aattgaataa
    33061 cttaagcttg gttgggcaat atgaagtatt ccggcagctt tcgtataatt ctctactttt
    33121 gctaaaacac gaaaataatg caattgttgt aatgtcatct caaccaaacc tcctaatatc
    33181 aaaataagcg gtaagatgtt aatactaagt gcggcttaat aattatagct attataccag
    33241 attatttata gaatgtctct ataattttaa aaaattttgt cattgctgtg atactatcat
    33301 catgaaccga agggtaagaa cctcaaggat gacagattga cagggtttga gcagtgacaa
    33361 gttgagggta atgtaggtaa atcttagtgt aaaacaaaat attgaatgat ttttattttt
    33421 ataatataat atttaaaacg tttgcattaa gatgatacat cttgtctttg tctcaaatag
    33481 atatacggtt attgaataac agggtggcag aatttcaaag ttcagaaaat tttgaatttc
    33541 tatggaaagc aggaatgaaa tggaaagtgt gaacaaatac aaatatatgg gtttcatatt
    33601 gcttcaaagt gtaatttatg ggtttggaaa tccacttact aaaattgctt ttaaaagcat
    33661 tactcccttt tggagtcttt cagttcgatt cacaattgct tttatagttt tcatgttatt
    33721 ttttggaagg cgtataattg gctctttaaa aagcgtagag ccaaaggttt atctacctgc
    33781 cggcatatgt atggcaacag cttacattac gtgtaatctt gcattgagct ggacctcagc
    33841 gacaaatgtc ggatttttaa tgagtctgcc tgtaatattt gcaccaatac ttgcactctt
    33901 gatatataag gttaaataca ggctgataca catacctgta caaattgtag tcgttatcgg
    33961 attatatctt ctctgctttg atggagaaaa attagttttt ggaaaaggag agattaccgc
    34021 attaatcaca gccatatcca tagcaggagc actggtattt ggagaagaat ctcttaagca
    34081 gctggatgcc gtagccgtat ctgctgtcca agccggcata actgctttaa taagcctgcc
    34141 ctgcgcattg atttttgaaa atactgaaat tttagtccat gttactatac cagcctggtt
    34201 agtggttttg tatctggcga tattttgtac caatattgcc tacatgcttc aaaattcagc
    34261 tctaaaatac atatcagcat caactgtgtc aatgcttcaa tgcacacagc ccattctaac
    34321 ggcaataatt tcttatttgc ttcttagaga aaagcttagc acattaggaa ttataggagc
    34381 tgcaataata atcttatgta taattgcaga aaatataatt actacaaaag aacagtctgc
    34441 ttccaaaaat cttgtctaaa aaaatagtaa ttttctaatc cctttctgta taaacataaa
    34501 taggggggat atttatgttt attaaaaaaa atcagtttta tattatcatt gcagttatag
    34561 tttttttaat ggctgtatat atgatttatt ccattaggaa ggacaatcgt ttaaacaaca
    34621 atgaagaaaa tgtgattaaa ttggtagagg attttggaag cactctgaag aatgtgcctc
    34681 ttttagcacc aagagatgaa gttatacaag caattaaaga taattatagt ccatatgtta
    34741 cggatgagct tatgcaaaac tggattgctc atccaaggag ggctccggga agaactacat
    34801 cgagcccatg gcctgacagg attgaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34921 nnnnnnnnta tattacattt aaggattagc actcaacgag tttaagtgct aacaacaata
    34981 gagataaagg agtgttgaac atggctggat tagtacctta taacagaagg aacagaggtt
    35041 tggttgacag aggtttcagc gattttaggg acttttacaa catgcttgat gactttttct
    35101 acgatgcctg gccatctaaa aaatactttg caagagatac atttaaaatc gacgtacagg
    35161 aaaatgacaa tgaatactta gtagaagcag aacttcccgg tgttaacagg gatgaagtca
    35221 atattgaaat gaacgaagga agactgaata tttcaataca aagagaagaa agcatcaatg
    35281 aagaaaacaa aaattacgtt cataaggaaa gacgtttcag ctctatgagt agaagtctat
    35341 atttagatga tgcaaagtct accggcatta aagcaaaact tgacaacgga gtattaagca
    35401 ttattgttcc taaggaaaac aaaccaaaca aatcagtaaa aattgatatt gaataatcaa
    35461 acttaaagca tgtggtcata atttccacat gcttttttta ttgaaaaagt atgttattat
    35521 tagtagtggt tcttagctgt aattgctatg aataacccgc agggtgtcat tctgaacgca
    35581 gtgaagaatc tcatgagatc cttcgcatac gctcaggatg acaaccggat tgtaaacaat
    35641 taataaaatt gaacaaaatc atgcagtatt tcgtcaatat aaaaaacaaa aaaaggagta
    35701 attatgtctg aagttttaag attagagcat gtaagtaaaa gtttcggaag caaggatgtt
    35761 ttaaaagaca taaatttcag tgtgaatagc ggtgaaatta tcggatatat aggttctaac
    35821 ggtgccggca agactacaac aatcaaaata attttaggtc ttatagacga ttatcaaggg
    35881 gatgttttta tttttggaga aaaagtaaaa ggacagtttg aatataaaaa aagaataggc
    35941 tatgtacctg aagtatctga tatgtatgat aatttaaccg catatgaata tttgagcttt
    36001 ataggaatgt tatacggtat aggggaagat aaggctgtaa aaaaaggaaa agaaatgatg
    36061 agtgtctttg gtatagacga tgccattaac ggcagaattc atacattttc caaaggaatg
    36121 agacaaaagc tgtcaattat caccggaatg attcataatc ctgatatttt atttttggat
    36181 gaacccttgg gaggaataga tgccaacagt gtattggttt ttaaagaagt attgaaaaat
    36241 ttaaaaaaag aaggtaaaac aattttttat tcttctcaca ttttggaagt tgttgagaaa
    36301 ctaagcgaca gaatactcct tataaatgaa ggggaaatag ttatagacgg aacagtggca
    36361 gatgtaatga gcatgcagtc ggattcatca ttagagtcaa tatttaatta tgttaccgga
    36421 tttcatgatt atgaaatgct tgcaaaaaaa tatacggatg tatttatgag ctgacggggt
    36481 ctgatatgaa tgaattttta tctttaaaaa tacttgattc tttcaaaaga atatttgaag
    36541 gcttttgcat agattataaa ttgcttcgaa ttatattgaa aaccaaatta atattggatt
    36601 caaggcgggc accggtgata aatacaaacc aaaagaagaa agatgaagaa aaggtttctc
    36661 ttttgaaggg atatttcatg catctgcttt ttggggcatt tttggcattc tttatttatt
    36721 ttataaaaga tgaaatgata cgcatgatgt atttttccgg tgcatttttc tttatgacta
    36781 ccatgtattt gatttctgat ttttcctttg tcttattgga tacaaaggat aaaaatatat
    36841 tgttgacaaa gccggtgaac tcaaggaccg tgtctttagc aaaagtattg catatactta
    36901 tatacatgtt caggttaaat acctttattg caggtccgtc tcttatcgca ttggtaatga
    36961 gcaacgattt atttgttatt ccgatatttc tggtggaaat attgcttatg gatttactga
    37021 tatttttagt gactgcattt atatatttat tggttttaag atttttcgac ggagaacttt
    37081 taagaaatat aatcaatttt attcaaattc ttttgacaat cttggttacc gtaggttatc
    37141 aaattgccat aagaatgata gattttactt cacttcgaaa tatggagtat acttttaatt
    37201 caagacattt tctgaatcct attttatggt tcggagcacc ctttgaaata ttctaccacg
    37261 gaggagaatc atacttatat gtttttacgg ctttaataat tattgttccc cttgtttctt
    37321 ttatagttta tttaagcctc agtaaaaaaa tggagagcct gcttcttaag ttagaagggg
    37381 aaggtaagga aagaccttca atacattgga ttgaaaaaat agtaggattc ataataaacc
    37441 ctaatgaaat ttcaaagcaa tcatttaatt ttgccaatgc catgtttaga agtgacagaa
    37501 gcttaaagct gaaaatttat cctgccttat cttccggaat acttcttccc atattaatga
    37561 tatttaatat gaggatggga ggaagggaat ttaagccttc cggttatgaa tatctttatt
    37621 tgtattttaa tttgcttata ttctcatccg tgttttattt agtgaaatat tcgtctgcat
    37681 ggaaaggttc atatgtattt atttcatcgg gatttaaaga ttataaaaat ttatacagag
    37741 gattaatgcg ctcaatggcc tataggtttt ttataccctt aattatttta aatggagtag
    37801 tatttacatt aatttttaaa tttagatttg tagaggatat agtaatttta tttttggcag
    37861 gtattgcaat tttgcctctt atcggtaaaa tatatttaga gattcccttt tctaaaccta
    37921 ttgaggaatc aaatcaaagg gattcttttg ataaatttct gctttcattt gctatagttg
    37981 caatttttgc tattgtgcat ttttactcaa atattattcc tatgggaaag tggatctatt
    38041 tatcggtact ggcagtcggg attccaataa gttggaaatt tgcaacaccg ggagaataaa
    38101 tattattggg agcatttatg gataaagtaa ctttatggac caggcaggat ataagaagtc
    38161 tggatgattt ggaaagaaag ggtgtaatca ggataacaag gaagcattta gaggagaaat
    38221 tcgatgttat ttccgaccat attatatttt tatataattg gtttgtcaat gaggcagaga
    38281 aaagagtgcc aagaccggag ggcgtggagt ttttcatctg gtgctccatt agcgaggagt
    38341 atatgctaag acctacatta aatgaaatag tatatgtttt ggaagtagat aaatctgagg
    38401 tagtatattt tgacggcatg aagtgggatt atgttctaaa tcatcactat gttccaaagg
    38461 atgaaaagga tgctgaagaa tatttaaaag acttagaaag aaagggtttt aagaattctt
    38521 attcatttat taacgaaaaa acagcccatt tttatccgca ggaaaggaaa agagtaatgg
    38581 atagctgggc tagagtgttt gaaattgatg aatgggatat tttcaggatt caagcaaata
    38641 tatgggaaat tcgccccgaa atgataaaag acatattaag agcagattct taaaatacaa
    38701 aatctactgt tacgttagcc tcaattctaa ttgtccccgg aattatggga gtagcagcaa
    38761 attctctttg aacaggaaga aaaggtattg ctgcattttc aacaatatta acaacaagag
    38821 caggtgtaga atacccaaga ttcattgtga ttgaattagc tttctgaatt gcatttaata
    38881 tagccatatt caaaacctgc tggtaatagt attccctgtt tgatatatca aaagttatca
    38941 aatcaacaac attggcacct gcgtttaccg cagcatcaat tatactgccg gcagcttcta
    39001 aattggttgt tcttatttgc aagatatttc taaccgaaaa acctctgtca atttgccttc
    39061 catcttcaaa atcatatact ctgtcaattg aatattgaga agtttttatg tcggtaattc
    39121 ccattcgttg aagtgtctga ataatagatt gacttattct tgcgttgtct gcctgtattt
    39181 gagataagtc ttctcccgta gtttgaaccc ccaaacgtat tataactatg tccggtgcta
    39241 tttccatctc gctatgacct gtcaatgtca tggttctgtt atgtacatta tccgatttat
    39301 attttgtcat cataatcccc cttttattta aaaattttcg aattatatta taatatgaac
    39361 tttattaaat tatgtgtcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    39481 ggtccatagg ggacggttct tgccggacat ttggagacaa tacgtcaggt ggttactgtt
    39541 atcacgttaa attataatca atttttaatg aaatagtata attattatgg taaaataaat
    39601 tagctaatat caaattggag gaattatgaa atttttgtgg actacaatac ttgtaaataa
    39661 tatggatgaa tcattaaagt tttatgaaga aatagtaggt cttaaaatga ctgagagatt
    39721 ccaggccgga cccgggatgg aaatatcttt tttgggtgaa ggagaaacta aggtcgagct
    39781 tatatacaat gagaaattta aaaagttgga tgcaggcaat agggtaacat tgggttttaa
    39841 agttgaatca ttggatgaaa agattaactt tattaaggaa aagggaataa agataataac
    39901 aggtcccata gaaccaaatc catcaatcaa atatttcata ataaaagacc ctgatggagt
    39961 aaaaattcaa tttgcccagc agctccaaag taattaagca aggtgttcat tttgataagc
    40021 ttaataaaaa aggatttaga caaactaaaa aaaagaagag aagattttaa ataataaaaa
    40081 ggtttaaaat cagcaagtac ggctcaatta aatacaagaa aagatatctg attaacatgt
    40141 aataattcta ggcaaaagca aggttacgag tggggatatt atgttaagaa tagcaatatg
    40201 cgatgatgat aaggaactat gcattgagtt aaaaagcatg ctgaatgaaa tcagcaaaaa
    40261 tacgggcgaa gcctttgaaa tttcggtttt ttgtaacgga gaaggactgt gcgattcatt
    40321 gatgagcgga aatcgcttca acttgatttt tcttgatata gaattgtgtg aaattaacgg
    40381 tgttgaagta ggaaagaaaa tcagggaaga attaaatgac gaaatcactc aaatagtcta
    40441 tatatcagcc aaggaaagct atgctatgga gctgttcgat atacgaccct taaattttct
    40501 tgttaaaccg ttaaagatcg aaaaaattaa atacgttctt aaaacagcaa ggaaagtatt
    40561 aggcagcagc aatcaatatt ttgagtatag agtaggcaat gtaaatttta atgtattaat
    40621 aagtgatata ttgtattttg aaagcagcgg cagaaaagta aaggttgtat tgaagaatga
    40681 agtaaacgaa tattacggaa aactttctga agttgaggaa aagattaaaa atgacgattt
    40741 ttttttcatt cataaatctt atctaataaa ttacaatcat gttattgaat attcctatga
    40801 atatgtgaaa atgtccaata atgaaatact tggaataagt caaaacaaca gaaaggcagt
    40861 tcgtgaaaaa ttgctgcgaa aaaagcagag gatgcgccat gtcaaatagc tatgttgctg
    40921 tttacctgat aacaaatatg tttgcaacat atactcttta cagatatatg tgcatatttt
    40981 tcgacaggaa ggatgttgac aaaaggcagg aatttttgtc ttattcatta tatttttgca
    41041 taaacagttt cttgtacctg acctttaata aacctgccct caacattgct tcaaatttaa
    41101 taatgttttt tttgcttaca tataattatc agtcaacaac aaaaaccagg ttgatagctg
    41161 ttgtgttaat atatatgatt ttaatgacta ttgaagttct tgtgatgctg atacttcaaa
    41221 attacggtat aaccgtaaca tccggaaatg atgcggcttt aattacaggg cttatcagta
    41281 taaaaattat ttcatatatt tttatgttgt ttatgtcaaa ttttaaaatg ataaataacg
    41341 aaatcaaagt gtctaatatt cactggctgt ccatttttat tataccggtg ggtaccttga
    41401 tattagtgct gattatgatt tctatggatt atgcttccaa tgttaccgga ataataataa
    41461 gcataatgat tttgctgatt attaatgtgt ttgtttatta cttttacgat gaacttatga
    41521 agtcatatca atcgaaaatt gaaaaaaccc ttcttcagca gcaaaataat gcttatttaa
    41581 agcagcttga aattattaat caatccaaag aaaacattaa aacattcagg cataatgtta
    41641 aaaatcatgc tttaacatta aagtattata tagacaatca tgacagggat ggcgccataa
    41701 aatatttgga caatatattc gaatttatta attattcaaa ggagcatgcc aaatcaggca
    41761 attttgaaat agacagcctt ataaattata agatggattt ggctgagaaa aacaatataa
    41821 aagcagaagt gtatctggca attccccata agctaaatat aaatcccttt gatttaaata
    41881 ttgttatagg aaatttgctg gataatgcaa ttgaagcagc attaaaggca gaaaacaaat
    41941 ttatcaatat atccatcgaa ttggacagaa atgttctata tataagtatt tctaattcct
    42001 atgacggcag actgaaactt ataaagaata aattggtaac aacaaaagac aacgataatc
    42061 acggcatagg attaagcagt gtacaaaatt cgatagaaaa gtataatggt accatgaata
    42121 ttcactatga cgagcgagtg ttttatgtgg atgtattaat gtacaattaa tattctatgt
    42181 cttttcatat ttgatgaaca aatattaggg cttagtttca tgtttaagtt ttgacataaa
    42241 ttcatattaa ttattacatg gtaagaaatt aggtatgtat ttgtgataca atggcattac
    42301 attatagagg aggacgatgc catgaatttt gaagaatata ttagaaaagt atatacaatt
    42361 gcatttagac ttacaggaga cgaaacttca gccaaagacg ttgcttctct tgctattgat
    42421 aaaaatgcaa atattgtaaa agaaaatatt gattcaaata tattacatat taccataaaa
    42481 gaagtttgtg gcatatttct taaggagtat agtatattca gcagtgaatc attttacaat
    42541 aaaaataata caactataca aaacaaattg atgaatttag agccgctgag caggacagca
    42601 gtggtatgga aggacataat gggatataat atgaaggatt tggaaacaat atcaaattgc
    42661 ccaaaatcag agctttatga gaagttaaac aaggccagga aaaatctcat ccacttttag
    42721 tcatccttcg cgcagctaat gatctcaaac gtttccatta taccatctta agctgcagcg
    42781 aaggatctca tttggaggac taatgaaata taaatgtcta atacttgacc atgatgatac
    42841 agccgtaaaa agcacaccaa atatacatta tccctccttt gtggaggcgt taaaatcatt
    42901 aagacctgat attgaactaa gccttaatga ttttgttaat tattgtttta atcccggatt
    42961 ttcattgctt tgcaaggata tattgaaatt cagcgataca gagcagcagt atcaatacaa
    43021 gatttggaag gattatgtca ataataattc tccggaattt tattcaggtt ttcctgaact
    43081 aataaaggaa tataaaagct taggcggaac aatatgtgtt gtatcacatt ctgaaagcca
    43141 gcatataatc agagactata ctaagcattg tggtcttaaa ccggatttaa tttttgggtg
    43201 ggaacttgat gagcatcaaa ggaaacctaa ttcttatcca ataattgaaa ctattaaaag
    43261 gtttaattta agaaatacgg aaatgctggt gttggatgat tcaaaattgg gtcttgacat
    43321 ggcaagaagc tgtaatgttg aatttgcagc agcgggatgg tctcactcag ttcccggaat
    43381 agaaaaatac atgagagaaa actcagatta ttatttttca acaattgatg aatttagaaa
    43441 attcatattg gactgcggga acctatatga tttcaaatag ttaaactcat acccggcatc
    43501 ttttaatttc caattttttg ttgataggca aaaccttatt tgatatactg gttatatcaa
    43561 tatttttttg tgcaaatgga aaggtggaaa taattatgtc ggttgaaaaa ttattacttt
    43621 caaaccttag caccatagaa gcagaaattg ttatcgcaaa attaaaatca tatggaatac
    43681 cggtattaaa aaaatcaaaa ggcaccggtg agctaatgga aatatacaca ggtgcaaaca
    43741 tgtatggaat agatttatac gtaccttcag acatgattga attggcagaa gaactgctaa
    43801 aaccggaaaa tgaaacggaa tagttgcgtt atctaatttg actataggtt gattacaaaa
    43861 aaaattacta ataaaaaagg aatgaaaaga caatgatgtt taaaacatgg atatggttat
    43921 taataatagc tgttttatgt atattgctta tatacgtagc aaaaaagaaa accaaatggt
    43981 ttggtttgat gctttccata ctattctttg ttgtttcaat ttttatagtt gtatattcaa
    44041 aaagcatgac atcatcatat ttaatattat tcagtatttt tataatagct aatattccaa
    44101 cagcaataca attatatatt ttttttaaat atcccgaaaa gtagaaatag ggacggtttt
    44161 acgaaccgtc cctaataatc ttgatctcag ttgattatta atatagtata atggagaaat
    44221 ctgaagatat actaaggata aaaatgttac taaagaggag atattattga agaaatataa
    44281 agtatggata cttctgacat catgtgtttt gttttggtca ggtaattatg ttttcggctc
    44341 attggttgtc accgaattga cgccggttgg tctgacattt ataagatggc tgtttgcatc
    44401 ggttatttta ttgatagttg ggcaaaaaat agaaaagcct gattggaagg aggtttgcaa
    44461 acagtggcct aaaattacaa tgctgtctat gaccggcatt ataggttata atataatttt
    44521 gtactatgct ctatattaca catcgccgct taatgccgcc ttggttaatt ctataaatcc
    44581 aagttttata gcaatagttt cagcatttta tttaaaagaa agaatcacaa aatatcacgt
    44641 ttttggaata atgatttcat tcttgggtgt atggattgta ttaaccaagg gaaatttgct
    44701 ccaagctttc aatatgaagt ataacagagg agatttgctt gtaatagcag ctgtgattac
    44761 atggacatca tattctgtaa taagtaagaa gcttacaact gttcccccca ttacttctac
    44821 aggtgtttcg ggacttatgg ctggaattat actatttccg tttgcaatga ccagcggagt
    44881 taattattta aatatgagta ccttggcatt gacagccgta gtttatatta ttttgttccc
    44941 gtcattatta tccttcgtct tctggaatat tggagtgagg gaaatcggtg caggcaagac
    45001 aggcatattt ttgaatttaa accctgtgtt tactgcattt atcagcaggg caatggggaa
    45061 ggatattaca tgggtgcagg ttataggggg actattagtg tttacgggag tttatataac
    45121 tactgcattt aagggtaaga ctgcaaggct caaatgagga ggttataatg gagaaaggtt
    45181 atattcatat atatacaggt aacggaaaag gaaaaacaac tgctgccttt ggtcttgctg
    45241 taagggctgc tttatccggc aaaaaagttt ttatagggca gtttgtgaaa aacatggagt
    45301 acaatgaaac aaagataaaa gattatttaa gcaatatcga aataaaacaa ttgggaaggg
    45361 gatgcttcat atacgaagat cctgaagaaa ttgacaaaga aacagcaaaa aaaggcttgg
    45421 aagaatgcag cactcttctg gcaagcggag aatatggact gataatcctt gatgaaataa
    45481 acatagccct ttattttaag ctgcttgaag tatctgatgt tgtaagtgca ttaaaaaata
    45541 aggccgagaa cactgaagtt gtgttgacag gtcgatatgc tcccaaggag cttattgaaa
    45601 tagccgattt agtaaccgag atggttgaaa taaagcacta ttacagccgc ggagtattat
    45661 ccagagacgg tatagatcac taaaattatt agggacggtt ctttttagta agaaccgtcc
    45721 cttaaaaatg aaatgtcatt tttttactgc ctttgttgca taaaaggtag gcatattcaa
    45781 ttcatggagc cttcctgaac cgtttgtatc ttcataaatg tcttctataa taaatccggc
    45841 ttttatttgt ccccctattt gttcctcaat agtatgagaa aattgaatac cccaatcctt
    45901 ttctaccgaa agctgataaa gctcttcatc cttcagcgga ttaaagggca gcttgcgtac
    45961 aggtgttgtt tcatcatcat cgaaaataaa attaattccg ttatctaatc ctgccaataa
    46021 tattcctcct tttttcaaca ctctgaagca ttccttccaa acaggaagaa catcctctat
    46081 atagcagtta gatacaggat gaaatatcaa atcaaaagtt tcatcctcaa agggcaaggg
    46141 ctttgtcata tcagccctta tagttttaat ctcataattt tcccttttag caacctcaat
    46201 ttcacttgca atttgtttct ctgaataatc aagtacagta cattttgcac ccaatgcagt
    46261 gaaaattggc atctgctgac cgccgcccga agcaagacca agaacttcgg cattcttcat
    46321 ctcgcaaaac cattcctttg gtacaggctt agtcggtgtt aacaaaacaa accaatcatt
    46381 gttttttgct ctttcaaata cttcgtgact gacaggctgc ccccattccc atccttcatc
    46441 aatccattta tcaatgaatt ttgaattaac ttctgtatac tttggagatt ttggagacgg
    46501 tttttttcct tcttcgggtt ttatctctga ttttttcata aaactatcct ttcagtttta
    46561 tatatcgttg atacaatatt atcatttata ttatgaaaat caatataatt tttatatgac
    46621 aaggggacgg gggtattgtc agctttttgt tttgagagcc cttagttaaa aaaattccaa
    46681 aaaaatatat tgacaaagtt gatatattgt atataatgga tatatacact atttacggag
    46741 atggaaatga atataataat aagcaattca agcggtcaac caatttacga acagattgct
    46801 tctcagataa agtctatgat tatcacaggt gaattaagag agggagaggc actgccttcc
    46861 atgagacttc ttgcaaagga gctgagaatc agtgttatta caacaaaacg ggcatacgat
    46921 gaacttgaaa gggaaggatt cattgtttcc ttcaccggga aaggaagttt tgtggcaggc
    46981 aaaaatatcg aattgataaa ggaacagcat cttaaggaga tagaaaacca catgcaggaa
    47041 atacttaaat tatcaaagga atgcggtttg aagctggatg aaatgattga aatgatgaca
    47101 ttgctttatg aaggagaata aaatggaata ctcattgaaa ctggaaaatg taacaaaggt
    47161 ctacaaggat tttaagttag acaatatcaa cattaccctg cccaagggaa gtataatggg
    47221 ttttataggg gaaaacggag ccggaaaaac caccaccata aaactgattt tggatttaat
    47281 cagccgggat gggggaaata tttcaatttt aggcaaagat aacaaaactg aaatggattc
    47341 cgttaaggaa aacatcggag tcgttttgga tgaatctagt ttccctgaaa atctaaacgc
    47401 aagagaaata aacaacatgt taaaaaaggt atataagacc tgggacgaag gcaaattttc
    47461 aaaattcctc gatatgttct cactccccta taataaaagc atcaaggatt actcaagagg
    47521 aatgaaaatg aagctgtcaa cggcagtttc catgtctcat gattcaaaaa tattaatttt
    47581 ggatgaagcc acgagcggcc ttgaccctgt tgtcagggaa gaaatcctgg ataccttttt
    47641 ggactttctt caggatgaaa caaattccat ctttgtatcg tcccacataa taagtgattt
    47701 ggaaaaaatc tgcgactaca tcacctttat tcacaagggg aaaattatat tctcggaaac
    47761 caaggacgat ttgctggaca attacggtat cttaaaatgc tctccggaag agtatgaaaa
    47821 cattgataag agccttgtga agggaatgag gaaaaacaaa ttcggaatcg aagctttggt
    47881 gcttaaggat aggatcacgg gaccttatct gattgacagg gcttccctgg aagatataat
    47941 gatatttatt ataaaggagc aagtgcaatg actggattga ttttaaagga tttgttaaac
    48001 ttaaagaaac aagggaaggt atatttaata ttaataatat tctattttgc cataggcctg
    48061 gtcaatgaaa actcatccat gttcgcctcc atgatgacta tggtggcagt attgatacct
    48121 attacggcaa tggcttatga tgaaaggtgc aaatgggaca gatatgccct tacaatgccg
    48181 gtatcaagaa aagacatggt tatgagcaag tacttgctgg gcttgatatt tttgaccgct
    48241 gcttttgtta tatcaattct tcttaacatg gttttttcca atatgctcat aaatgaaaaa
    48301 atcttgacgg ccttgggcaa cttttttgcc ggattgacat tgatggcagt catctttccc
    48361 ctcctcttca agttcggagt ggaaaaaggg cgcatcctga tgatgattgt tatattcata
    48421 ccggtggcag ctgtgatgat gatttcaaaa attggaatcc caatgcccgc agaggaaaaa
    48481 ttagaacaat tgatttattt tttacccata attggtgtac tcttatttat aatttcgatt
    48541 tatgtttctt tacggatata caggaaaaaa gagttttgat gcttactaat gggagaccgg
    48601 aaaagatcaa aaggaaggaa ggaatatgaa aaaagtttat gaatttgatg cagtgattaa
    48661 aaaggttccg gatatagacg gggcctatat tgagtttccc tatgatgtaa aggaggaatt
    48721 cggaaaaggg agagttaaag tatatgcaac ctttgacggt gagccctatg taggaagctt
    48781 agtcaggatg ggaactcctt gtcatataat aggcatcaga aaagatatac gggcaaaaat
    48841 caataaacaa ccgggggaca tagtccatgt gacaattaga gaaagaagtt aatagttgcg
    48901 taatctatag aataacaatt atcaaaaata gccgtacttg tggcaatgga caggtcgttt
    48961 taagcttttg agaataaaag gaactatccc taaaattttg gaactatttg ttaataggta
    49021 acgtcttatt gatataatac aataattagc aaaaaccggt ataggaggtt taaatggatt
    49081 ttaaaaagtt tatggtttta tttttgtgca tattattatt tgcttcgtgc tcagataata
    49141 atgtagttaa ggaaccggaa ccggtagaag agcctgaagt tattgaggaa gacaatatac
    49201 ctacagcttg gaacttgagt atggaagaat ttcgtgtaga cgttcctttt agtgttcctg
    49261 atgttatacc tgttgtggag aaatatgaag tcaatgaaga tttatctaac ctggtaaatg
    49321 caggccagta tgccggattt acagataagc agctaaaatc aatctatgaa gatggttttg
    49381 tcgtattgaa accaagctat gaatatttaa aaatgcatca tttatatgaa taccccatgt
    49441 ataaggaatc acctgtattt ataacagttg actccgctct ccatttatac cacatttttt
    49501 acggcaattc actaaagctc cttgaagtat cttcactgta tgataaattg cagtcccttt
    49561 caaaaaatat gctgaccgaa agcttaaatg cctataatga ttccaagtat gctaatttaa
    49621 aggaagaatt gaaatttgca gccgcatatt ttttaaccgg agcaaaattg attgatgaag
    49681 atttggaagg tatagtagtc cccgaggaaa ttgcagtttt atccgacgat gagataaagc
    49741 ttattgatga agcctttgat ttcgcaaggt ctcccatctt cggaaaggat ttggattatt
    49801 cccaatttac cgtaagaggc cactatacgg gaaatgaaga attaggtcaa tattttaaaa
    49861 ctatgatgtg gtacggctta agcggattcc cgatatttga tgaaagcaaa agcaagccgg
    49921 tccttgatat ggacagcttg acaaaatcaa tgataattac ctgtcttctt cttagaaatg
    49981 aagatagttt tgatgatttc gaaaatattt atactgcaac agctctttat accggcatgt
    50041 cagacgattt gggaattttc gaaataagag acttaataac aaaggtatac ggacagaatc
    50101 ctgatttgaa caaatttaag gataattcat actatgataa gcttttagag gaagcacttc
    50161 ttttgcctga accaaaaatt cagcctaaat attcttcagt cagtacacct gcgggaaggc
    50221 agttcaggct tatgggacaa agatattcct ttgatgcgga agtaatgcag gcactaatcg
    50281 agccaataat aagaccgatt ccttcaggtc ttgatgtgat tgcatccttt ggcagcaaaa
    50341 gagccgaaga gcttttggat acatactaca aaccaaagga agattgggat aaatacgaag
    50401 aaaatttaaa cctcatgcga aaaaaacaga ctgaaattac agatgatgaa tggaaatcag
    50461 atttatataa aggatggctg tggagcatta aatcatctgc cgtatccttc gaggataaag
    50521 aaggtatgcc ccatttcatg agaaatgaaa aatggacgga caaaaacatt catacagctc
    50581 ttggcagcta tgccgaacta aagcatgaca gcatcttgta catgaagcag tccggtgccg
    50641 aaatgggcgg aggacctgag ccaataatac cttataatta tgtggagccg aatgtggaag
    50701 tatatgcaaa gcttaagtgg cttgcagaaa atacgaaagc acagctacaa gaaagaaata
    50761 tgcttaagga tgaaatcggt ctggtattag accaaataat tgatattcaa gatactttaa
    50821 tgaatgtttc agttaaagag ctaacaaatc aagatattac agatgaagag aatttaaaac
    50881 tttacagata cggtggattg attgacagtg taattcaaat aatgcaaatg aatcttatga
    50941 ggaatgacgt tgatacaagc aatgatttca ctacggcatt aatagcagat gtatcaacaa
    51001 ttgcgccaaa cgacctcttt ccgaaaggaa cgtatttgga gataggaaac ggcctaccct
    51061 gtgaaatcta tgtagtatgt cagaccaacg ggaaaactta tttagcaagg ggagccttgt
    51121 ttaactatta tgaatttctt tccgacaaga gacttactaa tcacgaatgg cagaccttgg
    51181 tgggggtaaa gagaatggct atggtttatg acgaggaaaa gaatattcat gtccccatgg
    51241 atatatacga cgaagacgga aacaggattc tcgaagaaga tgaatatgat tttgagaata
    51301 taatgattat cggtccttca gaaaacatgg ttcctaagcc ggcatggaca gagtcattta
    51361 tatcacagga agaaaacaag gtaaccatta aggatataag tattagctgg gagtaaaatg
    51421 aagagactat taataccgct agcggtcata atggcagggg ctgtctgtgt actcattaga
    51481 cagcctgaat cttatgccgc cggaaaaata tcgatagaac agaataccta tggtttatat
    51541 gaaaaaagca attatttata tataaaaagt ggcaagaaat atgttaaaat cattgatgaa
    51601 gaaataattg attttaaaac atatgacatt gactctgaca aagatgatga gattttggct
    51661 atatctaaac atatttctga agaatacggc agtgatttca tcatatatga catatatgaa
    51721 gctgacggta tcatattgac aaaagaaatt tacagacagg attttttcga tttcaaacct
    51781 tggaaggttg atgcctgtaa tttggataat gacggtgaaa cggatatatt tatcggggta
    51841 tataaagaca ctgttttcta taaggatcta agaaagaggc cattctttta ttcttgggac
    51901 ggagaccgct tgcataagaa gtggcttggc tcatttttct ccgactggga tttggtggac
    51961 atagcttttg gcgattttta taatttaggc tatgatgtgg ctgcggtttt ggaaaaaaac
    52021 aaggatgcct gtagaatagg catttaccaa ttcataggat ttggctttga aaacatgaag
    52081 actataaatt ccgtggacaa ttcttttcgt tctcaaacag agctgctgtt tggcaaatag
    52141 agggttatta attaattttt aatagttctt taaggattac ataattttat gaaattataa
    52201 tagatactaa aattaagtcg atctgcaatt tggagggatg atgaaaaggt tttttacacg
    52261 taagaaggtt atcgtttttg ttgtggttgc ggctcttgct gcaatcatat tttggcttgt
    52321 aaacaagggt tccaaggcgc cggaagtgac ggcaaatgtt tcaaaattgg aaagaaaaac
    52381 cttagagcat gttatttcgg tgaaagcacc cctggtaggg attgaaaagg cggaggttac
    52441 atctccttta aattacaaaa taaacgatat aagggtgaag gaaggagatt tggttaaaaa
    52501 ggacgatatt ttagccgttt tggacagcga ggacctggaa aaagaaatag aatatgaaga
    52561 aaatcaaatt gaattaagca aaatggaagc agaggagaaa atcaaggccc tgcagattga
    52621 atacgacaag gctttggcaa gctacaggga tatggaaaca gtatatgagc aaaacaagga
    52681 cctgtacgaa aacggcgtaa taaccgaaga agcctttaaa aagacagaaa aggatctggt
    52741 tgttttaaag aaaagcctag aaggttataa tgccgtcaat gggaaaatac aattgtccca
    52801 agcagataaa aagcgcattg aaatacagga agaccggctt aagttaaaga aagaagaact
    52861 tgaaaaggtc atgataagaa gttctattga cgggaccgtc acgagggtaa atgtcaatat
    52921 cggaagatat gccaaggata ccgaaaacag ccaggcaatg tttgtcgtgg aaaatctgga
    52981 aaagctgcaa atgaaggcag cagtcagcga atttgacgtg ggaaaactta aattgaatca
    53041 ggaagtagaa atttattctg atattttggg taagggattt gcaaaaggaa aagttgcgag
    53101 aatatctcct acagcagagc aaaaagataa taacaccatg gaaagagtga ttcctgtttt
    53161 aatagatgtt acggaaaggc cggaaaattt aatagcagga gtaattgcca cagctaaaat
    53221 aagaaccgaa aagaaagaaa atgtatttgc ggtcccatcg ggagccatag tgcaagacgg
    53281 ggaggaatac aagatttaca tattgaatga agacaatacc gtaaggtcca taccggttga
    53341 aattggtctt gaaaccgatt tggaatcaga aatcacgggt gatttatacg aggaaatgaa
    53401 atatgtgata aatcccgacg agacattcga agacggtatg acagtaatac ctaatgaaat
    53461 tgagtaggtt gatatggaaa agatgattag gatagaaaac ttgaataaaa tctacaaaaa
    53521 cggctccgtg gaagttcatg ccttaaatga tgtgaatatg gaggtctccc ggggagaatt
    53581 cctggctgtc atggggcatt ccggctcagg aaagtcaacc ctcatgaata ttataggttg
    53641 tcttgacaga ccaagcagcg gcaagtattt tcttgatgga attgaaattg ataaacaaag
    53701 ccccgatgag ctttctttga taaggaataa aaaaatcggt tttgtttttc aggcatttaa
    53761 tttaatcccc aggaccaatg tgcttagcaa tgtggaactt ccaatgatat atgccaaagt
    53821 caagtcaaaa caaagaagtg aaagggcttt ggagctttta gagaaggtag gcttgaagga
    53881 taggataaac catatgccaa atgaattatc aggcggtcaa atgcaaagag ctgccatagc
    53941 cagagccttg gcaaacaatc ctccaataat tcttgccgat gaacctacgg ggaatttaga
    54001 caccaactct tctgaagaaa tcatgaaaat attcaaagac ctcaacaggg aaggaaccag
    54061 catcatagtg gtcacccatg aacctgaagt ggcaaaatat gcagacagaa ttattgtgtt
    54121 taaggacgga tgcattatcg aagacagatg gaaagagggt ggaagaaatt atttggcttg
    54181 aaaatattaa gatagctttt aaatctattt tatcaaacaa aatgcggtct cttctcacca
    54241 tgctgggaat tatcatagga ataagctcgg ttatttcaat cgtgtccata ggcgacagca
    54301 tgaagggggt aatggacgat atatacaaga atttcggcaa aaacagggct gttttgtatg
    54361 tgggaaacat agaggatttc agaaccacgg acttttttta taacgatgat ttagaaattt
    54421 taaaggaaag attcagtgaa aagttggctt atatttgccc ttccgattcc ttgagaagcg
    54481 atgttacggc aggcaggaat accataaaac tcaatatgga attcattgac tccggatacg
    54541 ataaagtcca acaaattaag atgctttacg gcaggatgat aaatagcaag gatatagaaa
    54601 gaaagagcaa ggtagttgtt ttggagcaaa aagccgcctt aaagctcttc gggaaggaca
    54661 atgctgtagg aaaaacaatt agggttaaaa tcgacaacaa tatggaagac ctcctggttg
    54721 tgggagtata taaaatagag caatcccctc ttttaacctt gatgcaggga caaagcaatt
    54781 atgaaaacag cttcatacct tatactatgc ctacttcatc atattacgcc tattatgcct
    54841 tggatatttt tattgatgaa aagtattcca tggccattac gggtgatgag ataataaatt
    54901 atattgccag aatgaaaaac aggacaccgg aaaactacat atattatacc gttgaggaag
    54961 aacaaacaca ggtggaaagt gttgtgaccg gcttatcagt tgcggttgca gccatcgcag
    55021 ccatatccct cctggtgggg ggaataggca taatgaatat catgctggta tccgttactg
    55081 aaagaacgag agaaatagga ataaaaaaag ctctcggcgc aagaacaaaa gatgttttgt
    55141 ttcagttttt gatagaatca gccacacttt cagcaatcgg ggggatagtg ggaaccataa
    55201 cgggcatagg tttagttatg atagggggtt cttttatatc catacccgtg gttgtaaatc
    55261 cggcttctat cattttggct gttgtatttg ccatggtaat cgggatattc ttcggctatt
    55321 atccagccaa gaaggcagcc aattcagacc ccatcgaagc tttgagatac gaataataac
    55381 aaaaaataca aaaaaggagt gatattgtga tattgtcgga agaacaaaaa aatcattata
    55441 aaagacaaat aataatacct gaaataggtg aacagggtca aaaaaagctt cttgattcaa
    55501 ctgttttggt gtattgcaaa aatacagaca gcctaagacc cttggcatat tatcttgctg
    55561 ctatgggaat aggaaaaata ttttgtgatt tacaagataa aatcggcgct gactccttat
    55621 ttgctgaggt aattgattta aacaatgata caaaaattga ttatcttaat gaagaaaact
    55681 attcgggagg tttttcggag atatatgaaa ataacaagaa ccatccccaa aatttttgca
    55741 ggattgtttt gggaaattat agttttataa aaaattcagc agctaaatta ttaaacgata
    55801 aatatttacc gactattata tcagctaaca gccaatggaa gggtacactg cagacattta
    55861 tcaatgagga tttgtttaag aattttattg cattaatagc agataataat acaaaagatt
    55921 ttagtccgcc ttttttagcc aatgcattat ctgcaacgct ttcttctata gaatgtgtta
    55981 agctttgcct taatatcggt aatataacaa aagatatgct gttttttgat ttgtttaata
    56041 tggaatttaa acaaacagat aataatgcta acttgtttga atttttaaat gaagcaaata
    56101 aaatggatat taaacaaaaa ttatctgatg caaaagtctt gattgtaggt gtgggaggct
    56161 taggctctcc ggcagcctac ggaatggcaa tggctgatgt caacacattg gggttattgg
    56221 atttagatga agtggaaatg agcaatttaa acagacaaat acttcattcc ttttcacgca
    56281 tcggtatgcc gaaggcaagc tcagcagaat ttatgctaaa aaagctaaac ccaagcataa
    56341 atattaaaac ctataaaact gaattaaccc gagataatgc agaaaaaatt tttttggaat
    56401 acgacctgat tattgctgcg gttgataata taccttcaag gtatctgata aatgatatat
    56461 gctttttatt aaataaacct tttgctgagg caggagtttt gagatttgac ggcactgcaa
    56521 ccacccttgt tcctaaggaa ggacattgtt acaggtgttt atatcccaat gtggacagca
    56581 gcaatttatc aggtaataat ggtgtcttag gttctgttcc gggagttatg ggttttatac
    56641 aagcggcaga agcagtaaaa atattgtccg gatttggaaa aaccttaaaa aataaaatac
    56701 tcttgtttga ttcattggca atggatttta atattattga tattgaaaaa aatccccgat
    56761 gtcctacatg tggaaaataa cctattattc tcatttatgt aagaaatttt ttaactgaaa
    56821 aatttctaaa tatttaaatt tttacctctt tatattgagt tatttctgga gatactatta
    56881 aaaaataatt cattatggag gtgactatga aaaaatttaa aaaatttaca attatttttt
    56941 gtgcattaat gcttttattg gttgcatgtg cacctggaga tggaaaccta aatgaggacg
    57001 aagacaacga gcctattgat gaacctattg atgacaataa taccggagca aatgcaaata
    57061 atgatacctt tgacgaagaa gaaaaaacat tgacaggatc tgcacaagga tatggaggag
    57121 aaataaacgt aacagttgta gttaacggag aggatatagt atcggttgaa gctgaaggaa
    57181 aagatgaaac tgagggtgta ggctctttgg caatagatga acttcctgat aaaatagcac
    57241 aagcaaattc aacagatgtt gacaatgtat ctggtgctac gaaaacaagc gaaggaataa
    57301 agcaagcggt taacaatgca ttacagagta ataaacaatc aaaacaatag caaaatgcat
    57361 atgaaaaacc agccgacagg ctggttttaa tcatataagg aggataaaat caatgacgtg
    57421 gatgattccc tgatacattc gattttatgc agctcgtctt gaatagcttt taaacctgaa
    57481 ggtgcactgc atcttacttt cataagtatg caccaagtgc cggtgactat atggcattct
    57541 tcaattacta aatcaggaat agccaagttt tttaattgtt ccaaaagctt atcataatca
    57601 gcagttctca ctcttaatga aataattgca tatgtggtaa aaccaagttt tgaccagtct
    57661 atcattgcct tgtatcctgt tataataccc tgatcttcca attttgcaac tctgttatgt
    57721 attgcaggtc ttgataaatt taatcttttt gaaagctctt cgtgtgaaat tctcccttcc
    57781 ttttccaagt gtttaattat ttgcatgtct aagaaatcca tatctacctc ttgtcatatc
    57841 gtaaattatt tgaagaataa aaaactaatc agttataata tagcacaaaa aggtacaaca
    57901 tgtaaataat tatttaataa atttttaaat aatgcatatg aatttagaat tcgaggcgag
    57961 ccgggaattc atgatttaag cctttatgaa tctttaaact ataaaaagta ttatcgctac
    58021 aattaatatt gcatgaatat atagtattat tttggcaaat ataaataata cataaatgtt
    58081 aaattggagg tatttatgaa aaataaaata atattaatat taatttgttt tttgctgttc
    58141 ataacaggat gtgccgtaaa tgataatcca ccggaaaaag agaccgagcc atatggtaaa
    58201 tatactgcca atggaagaat tgttaaaata gataaagacg gatttcatgt tgaaggtgaa
    58261 gataatgtgg gactttacaa agttgatatg gagaaaaaca aaaatttcta cataggtgaa
    58321 cgcgtaagat taaattctat ggatggaaac agttatgatg ttgaattaga cgaagaatat
    58381 gattacactg ccgcaatgac tgcagagatt tttgatgatg attctaaatt ggatcttaaa
    58441 gttggcgaga tatcgagaga tgaaatggga agaatgagga tttacggact ggcagctgac
    58501 gataaggaat atgatataat agtgggcgcc gatacaataa caaattttgc tcattcaaca
    58561 ttaaaagtag gcgataatat atatgtatat cctaaggatg taccgaacgg tatacctgca
    58621 gtagttgacg caaaagctgt tacagtggta cgttaatata atttctgcac tgattgacaa
    58681 aataaataat gtgttataga ataaaaatac ttcaagaaaa tggatgttag gaggtaagtt
    58741 ttagcgtttt attatgctga aatttacctt tttttaaggg aggaaaaatg gataaggtta
    58801 taattacagg aaatgatttg acattggacg aagtagtagc agtgtgcaga aattataaaa
    58861 aggtagaatt atcagatttt gctgttgaaa ggattttgaa atcaagaaaa gttgttgatg
    58921 actttgttga aaatgaagat gtagtctacg gtattacaac aggcttcgga aagttcagcg
    58981 atgtaaccat atcaaaagaa gaatcaaaaa cactccagaa aaatttaata atatctcact
    59041 cggtaggtgc aggaaaaccg tttgacagtg aggttgtcag aggaataata ctgttgagaa
    59101 taaacaatct ggcaaagggt tattcaggtg cgaggcttga aacaatacag acaatgattg
    59161 aaatgctgaa cagaaaagtt catccaatca tacctgaaaa aggctctctc ggtgcaagcg
    59221 gggatttagc tcctctgtca cacatggtgc tgcctatgat agggcttggg caggcggaat
    59281 acgaaggaga aataatggca ggcgaagaag ctatggaaaa agcgggaata cccgtaattg
    59341 agctgacagc caaggaagga cttgctctta taaacgggac acaggttatg actgccctgg
    59401 cagcattgac tgtttacgat tcgttaaaca tcataaaaac agcggatatt gcagcagcgt
    59461 taagctttga agctcaaaac ggaataatcg atgcattgga ccaaaggctc catgatattc
    59521 gtcctcacaa gggtcaaata gatactgcaa gagtattgac tgagctgttg acagggagca
    59581 aaatgacaac caagcaaggg gaaattcgtg tacaggatgc ttattccttg cgatgcgttc
    59641 ctcaggtgca cggggcaagc aaggatgcaa taaattatgt taaggagaaa atagaaatag
    59701 aaataaattc agttaccgat aaccccataa tattccctga aacaaatgaa ggtatttcgg
    59761 gaggaaattt ccacggacag cctatggcac tctccttaga ctttttagga attgcacttt
    59821 ctgagcttgc aaatatttcc gaaagaagaa ttgaaaggct ggtaaatcct acacttagcg
    59881 gattacccgc atttttggta gagcatggcg gattaaattc ggggtttatg attgttcaat
    59941 attctgcagc tgccttagtt tccgaaaata aaatattggc acatcctgct tctgttgaca
    60001 gcattccatc ctctgcaaat caagaagacc atgtttctat gggaacaatt gctgcaagaa
    60061 aagcaaaaga aattatggaa aatgtgcgaa gagtcttggc tatggaaata atgtgcgctt
    60121 gtcagggaat agacctaaga gggaacaaag gattaggagc gggaacaaaa ccggtttacg
    60181 atgaggtaag aaaagttgtt cccatgctaa aagaagacag accaatttat gacgatatta
    60241 ataaatgcga agaattaatt aaaaacggga caattacaaa ggcttgcgaa ggcagaataa
    60301 gatagatgct aatcatcctg ctgccttttt attgattata attaacttga atgttataat
    60361 taaacaaaat caaaaattcg tggaacaaat taaaaaatgt ttacgtctaa tatataaccg
    60421 aacggggtgg aataatgaat aataatgaaa ggtggcttgt agaggaaagt cggaaaggta
    60481 atgttgaagc ttttgaagag cttatcaagg attataagaa aactgcctac aacattgctc
    60541 ttcgcataat gagaaatgtt gaagacgcag aagatgcttc tcaggaagca ctgataaaaa
    60601 tatttaaaaa tataagcagc tttaatatgg aatctacatt taaagtatgg atgtatagaa
    60661 ttatagtaaa cacatgtata gatttcaaac gcagaaaaaa tataagcaca gtatctatag
    60721 atgaaacaat ggatttaggc tccggaagag aagttcaaag agaaatttca gatgaatcct
    60781 ataacccgga tgctttaatc gaaagaaatt acagtaccca gcttgtaaac gatgcaatca
    60841 atgcgttgga ggatgatttt aaaacaataa taatactgag ggatataaaa ggattcacat
    60901 atgatgaaat atcacaaata ttatcctgta atttaggcac ggtaaagtca agattgtcaa
    60961 gagccagaaa aagattaaga gaactattgg aaaatgaaat gaaagttcag ggctaaggtg
    61021 gtgataaaat gaaatgtaac gaaataaatg acaaattgtc attatatatt gataatgaat
    61081 taagccatga agaaatgcag caagtggaag agcatttgca ttcatgcgaa aaatgtcaaa
    61141 aaatcttgga agagtataaa aatttaatat cagtattgcg aaatttgccg gaagaagagc
    61201 cacctgtagg ttactgcaaa agacttcatg aaaaattatt ggagagcaac cctcagtcaa
    61261 aaacaaaaaa ggcagcttca aagataaaaa gtttcaatcg caataaatgg ctgaaatacg
    61321 gcagtcttgc cgctgcactg gtattggtgt ttgtagcggc caatataaac aatataggca
    61381 gaatgggaat gaaagctaaa aatgaatcct atgatatggc aacaactgaa tcaccagccg
    61441 aatactacgg tgccggctcg cctgccccag aagcacccca agcaggaccg ccggtaatga
    61501 ctgaagaagc tgcggaagag gggtatgacg gtgataataa gagcgcaaga gacgaccttt
    61561 catatcggac aaatgattct gtaatgatga cttcaatgga acaaagagaa atgaaaataa
    61621 tcaagaccgg aacaatttca gcacaaacag aagattatga catgttttta aacgatttgt
    61681 caatgaaagt aagttccatg gacggattct tggaaagcaa taatactcag gtttaccaag
    61741 tatataatga gaaaaaatta atgtatggga gcattagaat aagagttcct caggaaagtt
    61801 tttatgaatt gatcgattat ctggaaaata ccgttgatat aaaaagaaag aatatcaatg
    61861 aaacagatgt aaccaaggag tattatgaaa aggacaataa agtaaaaaac ttggaagttc
    61921 aggagcagca cttaagagaa ttgtttgaaa aggcaacaac ggttgaagaa atgcttctca
    61981 tagaaaatga gcttagaaga atcagaactg aaatagatgc tttaaatatt agtttaagcg
    62041 atataaatga cagggctaaa atgtcaacta ttaatttgga aattgaagaa gtgatgacag
    62101 caagtcttac cttaaaaact aaggacagtg tgtgggacag atcgaaggaa ggttttatta
    62161 atacagttaa cggaataata agagccgtgg aaaacttaat tgtaggcata gtatcaagtt
    62221 cacctatact ggtaccggca ataatcattt gtatagtagg cttccttaaa ataagaaagt
    62281 attggcatcg aaaaatttaa cagtttgaca tcctgtgcag ttgagacatt tctcatgcat
    62341 tgaagacaca ccttttattc aggtgtgttt ttgttttaca aaattctgtt tatagatttg
    62401 aataagtatg gtataatgat taaaatcaat gaagcaaaga ctttggtctt tgccctaatg
    62461 cattcattgt atcggtgtga agcaagtcca ttttgcggct tgcacctatg atacaattaa
    62521 tcatcaaatt aaaagtgagg aaaattatga gccaaagtat tgctgatatg cttagtgatg
    62581 aatcaagaga acagatgaaa agtatgatta aaacaaataa aacaattgcc gagcttaagc
    62641 ccggagaaaa ggcggacggg ttctatttga ttaagagtta tgaagttaaa aaaactacta
    62701 acggtaaaca atatatagat attgagctgg ttgacaagac aggtgaaata aatgcaaagg
    62761 tgtgggatta ctctaaagaa acggaagtat taattgagaa aaaccccatt gtaaaagtaa
    62821 ggggagaggt cttggaatgg agtggaacca gacagcttaa agttaataaa ataagagaga
    62881 agaaggctga ggataatgtg cagattcagg aattaatacc tgctgcaccg attgagccga
    62941 gcgaaatgct tgaaaaagta aaatcctata taaataaaat aaaggataat gaaattagaa
    63001 ccttaacgga taatatcttt aagaaatatg agaataaatt gtattattac cctgcggcaa
    63061 aaagaaatca ccattcatac aggggaggac ttttgtatca taatcttcga atgcttcagt
    63121 caggcgagaa gctttgtcag gtatatgatt ttcttaatac ggattatgta tatgcaggca
    63181 taattcttca tgatatgtgc aagatattgg aaatggaatc cgatgaatac ggaatagtat
    63241 ctgattacag catggaaggt aaattgctgg gacatataat tcaaggggta aaggaaattg
    63301 aatttgaagg tgaaaagata ggtatagaca gagaaaaaag cattattttg cagcatatgg
    63361 ttttatcaca tcattttgag ccggagtttg gcagtcctaa gaaacccatg acaccggaag
    63421 cagagctttt gcatttcttg gatataatgg atgcaagaat gtttgatttt gaacactttc
    63481 ttgaaatggt cgagccggga caatttaccg ataaaatatg gcttttggat aacagaaatc
    63541 tttataaatt aaaaggagac aataaagatt cttaacccct taggattaag aatctttttt
    63601 tatcttcttc tttgtcttat gcgaattggt ttttgtatat gtttttcact ccttatgttt
    63661 ttttctgctg aagggggcgt gttaatagtt ttattattta tttcatcgtt tttcttgtgg
    63721 tcaaattgag ctttcttaaa tacttcttct tcataaggtt tatttggctc ttctgtttta
    63781 tttgaagaag acatcacatt gtttatcatc ggcataaatt tttctattaa attgcttaaa
    63841 tcagtatttt ccttgttgcc atccgaaaaa gaagaaagca tatcacttag gttattgtta
    63901 ttgctgtttg ttaaagaaga aagcatttcg tttaaaccgc cgtttttagc catattcatc
    63961 gccaaggcac ttactatttt ttgcatattt tcggctataa gtggttgtgc cattaacttt
    64021 gtaacaggaa tattgccgtt tttcaacaat gctgttactt tatccgccgg tgttttggcg
    64081 tctatagctt ggattggcct aaaattctga gccttgctga taaatgagta catgccgagc
    64141 aaagtgcttg ctttctctac tgccggcaga tatgtattta cttttgatac tgtttgcggt
    64201 gaagtaaggg gagcaaaggt tcgaatcatg cctattaagt tttccatctt agccgggtcc
    64261 ttgagtatcc ccgaggccat cgttttagtt gttcccgaaa gcttggaaac aacaccgctt
    64321 aattgattgc cgtcaaaaat atattttgcc gctaatatat ataataatat ttgaaccgga
    64381 ataatatcac cttcttataa atatctccat atatcatatt ccgattgcat gattatgtta
    64441 cagagcaaac tgtaattgta taaacactat tgtaattaaa tggcaccggc ggcatattat
    64501 ataacatata ggaggtagat gaatgtcaaa tatatttaga agtgacaatg aaattaataa
    64561 aatcaaagat agcaaagaat ttaaggatgc tgaagctaaa gcaaatgaat tagcaaa
//
LOCUS       DAOSHP010000002        54764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_444_length_54764_cov_8.556563, whole genome shotgun sequence.
ACCESSION   DAOSHP010000002 DAOSHP010000000
VERSION     DAOSHP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 54764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54764
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_444_length_54764_cov_8.556563"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLB33_00350"
     CDS             <1..328
                     /locus_tag="PLB33_00350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG61999.1"
                     /translation="FLSFDKVLLMLFQLAFLALGEEIAWRAFFQKQLNKTFSIIHVLL
                     ISSLLFAIGHFNQGNPVIVIYDIFFVFINSILYGIIFYKSKNAWVSAISHFAANLFSV
                     IVLVFI"
     gene            383..1156
                     /locus_tag="PLB33_00355"
     CDS             383..1156
                     /locus_tag="PLB33_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62000.1"
                     /translation="MINFNKLPLMIVIAVMAVVILSACDGVNSNSNETDDIITDENTP
                     TTETTAVPFPWGGNIDGKEMTLDDVHQLAGKGDDLLFEDLWQYKGGNTSSSHERYIMV
                     YKVEGGYRLVVHSDSTGKPDMVNLESIWESGGSGIDIRYNDVYEFLRANPSQDGITEE
                     EAQDIGQTKLTMELEPVSWYILGDWPESTDENAVFAEALLDSIYTIDEPCWVFRVKGT
                     AEWGGSYYAVGKKSGTIFICSFDDNSKPIWSVVDSESNS"
     gene            1246..1479
                     /locus_tag="PLB33_00360"
     CDS             1246..1479
                     /locus_tag="PLB33_00360"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOG62001.1"
                     /translation="MHHVTVNDESGREARLCDYDWVLDIREQYKKYDITFWFKGTGSL
                     FKHDGTIKKINPFKQGSHAKKFDINIKNSGDRA"
     gene            1530..2294
                     /locus_tag="PLB33_00365"
     CDS             1530..2294
                     /locus_tag="PLB33_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62002.1"
                     /translation="MDNLERIAQFEKFSPMLYLDYKLTSWGRMLCDIGNKESYTSNYA
                     VIYRNENVDNIIKEVEEYYNSRSIIPKIFNRCGSVELDILRPFFTKHDYAIREFDMDL
                     MILDTHSTRNKIAEKVEVKIINRALEGQEYNLAIEQDNGETYGVKLLNKQIASGNNMF
                     FAYNELKKPVSMALAEKYNDVVYITDVYTTPSQRCNGYGTAVVYAVLSHYHNSLIYLH
                     TDNPDAASIYRRLGFSGKRLKSWWAVKGSLPEWCLE"
     gene            2282..2407
                     /locus_tag="PLB33_00370"
     CDS             2282..2407
                     /locus_tag="PLB33_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62003.1"
                     /translation="MFGIISTESRYEFVGDSMEKLQDDYNLITGVYIGDVLDVHL"
     gene            2448..3080
                     /locus_tag="PLB33_00375"
     CDS             2448..3080
                     /locus_tag="PLB33_00375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG62004.1"
                     /translation="MLDSKGFDLWAHGYDKSVNLSEENNEYPFAGYKDVLNYIYNQVR
                     KHNYSSVLDIGFGTGILTTQLYNEGYHITGIDFSRTMIDIAKSEMPEAILINWDFTRG
                     LPVEIKNNKYDYIISTYAIHHLPDEEKIKFIKSLLKLLNVNGKILIGDVSFMTRKELE
                     ECKETHTEIWDDEEFYFVANEMMMSLNEFYCNYEKISYCSGILIVADYLL"
     gene            3153..4184
                     /locus_tag="PLB33_00380"
     CDS             3153..4184
                     /locus_tag="PLB33_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG62005.1"
                     /translation="MLKKPNRLNRGDKIALVSLSWGGAGDENLLWRYKQGKERLEQVF
                     GLEVVEMKHTLAGTEYIYNHPEKRAEDLMNAFQDNSIKGIIACIGGIDSIRMLPFIDF
                     DIISNNPKIFMGYSDTTTTHLFCYKAGISSIYGPTLLVDFAENVSMSPYTVEHIEKTL
                     FKSEAIGEIEPSPKWTSEYLPWEGKNKLIQRKFKDNSGYELLQGKGITKGRLIGGCIE
                     VFDSLRGTEVFPKLEDFDDSILFLETSEEKPPAWIIECSLRIYGINGILDRIKGMIWG
                     KPQDETYYAEYKEAIKKVMKEFGKEDLPILYNMNFGHTEPKICLPYGAMAEINCDKSS
                     FSILESAVL"
     gene            4222..4593
                     /locus_tag="PLB33_00385"
     CDS             4222..4593
                     /locus_tag="PLB33_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002119671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HOG62006.1"
                     /translation="MEIKVIKINNVRIAKIYSNEILIKDGQSALDLLATVSYETDCDR
                     IIIDKSAIAEDFFDLSTKLAGEVLQKCINFHFKVAIIGDFSVYKSNSLKDFIYECNKG
                     KDIFFLPDENQAVEKLSIVRR"
     gene            4711..6030
                     /gene="rsmB"
                     /locus_tag="PLB33_00390"
     CDS             4711..6030
                     /gene="rsmB"
                     /locus_tag="PLB33_00390"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOG62007.1"
                     /translation="MKSYRAQAIETLKKIFKDKSYSNIVINNDMKNVDNRFLSLYRKS
                     VLGVVENLIFIDWIINQVSKTKTKKMETDVLLVLRLAVYQIFFLDKSHENIVVNESVQ
                     YIKDNGNIRASKFVNAVLRNILRSKDNLIKKMNELPRDDYLSVKYSYPKELIVKWEKQ
                     FGKDVDKVLIANNTEAPLEIRVNTLKIQRDELIKLFEEKGIKSYKCKYADKGLIIENP
                     FEIDKTDEYKNGLFSVQSESSMLAGQILNPKENSFIIDVCAAPGGKTLNAAEMMNNTG
                     KILSRDIYPAKLRLIENELKRLVIKNVKVEKYDARKLDESLVGKADYVIADVPCSGLG
                     IIRRKPEIKYNETDLKDITEIQYKILENASKYLKQSGELVYSTCTTNKEENIEIINRF
                     LKNKNFILADFSDIINEDFKTAKDGYIEIYPHLHGMDGFFIAKLKRL"
     gene            6081..7109
                     /gene="rlmN"
                     /locus_tag="PLB33_00395"
     CDS             6081..7109
                     /gene="rlmN"
                     /locus_tag="PLB33_00395"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOG62008.1"
                     /translation="MKKIDDLSFEELKDAVLKIGEPKFKANQIFDWIHNKTVESFDDM
                     TNLSQKTKAALKKEYEFTKAIIELKLESKLDETKKYVIKLEDNNIVESVYLKYKFGNT
                     ACISSQVGCKMGCHFCASTKKGFIRNLTAAEMLKQIYLIQNDINEKISNVVIMGSGEP
                     LENFDNIVRFLNIINDERGQNISMRKITLSTSGIVPNIYKLADENIPITLAISLHAST
                     QEKREQIMPIARKYKLPEILKACDYYVKTTGRRLTFEYIMIKGFNDSREDAENLSRLL
                     KNKLANVNLIPCNYVKEANIEPSEDLDIMKFKKILTDNGINATVRRELGSDINAACGQ
                     LRNDLLKM"
     gene            7118..7870
                     /locus_tag="PLB33_00400"
     CDS             7118..7870
                     /locus_tag="PLB33_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOG62009.1"
                     /translation="MDVFFDTNKGLMRENNEDSLIVEQFDNYCLYAVADGMGGHKAGE
                     VASSIAIEEIRNFFVECMKDENFQPPSFIMESVKLSNDKIREVSLNNEDCCGMGTTVT
                     MAVIDKLNNIIYIGNIGDSRAYLLRNNEIKQITTDHTYVNELIKDGRITEEEAKTHPK
                     RNVITRALGSEEYVHADIFELELLKNDILLLCTDGLTTHVSDDNMLEIIKEYGTSESV
                     QKLIKQANDNGGTDNITIIIVDNIIRGETYDR"
     gene            7860..9821
                     /gene="pknB"
                     /locus_tag="PLB33_00405"
     CDS             7860..9821
                     /gene="pknB"
                     /locus_tag="PLB33_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOG62010.1"
                     /translation="MIGRTLGNRYEIIEKIGGGGMSNVYKAKCNVLNRFVAIKILRDE
                     LTQDSDFVNNFKQESLSAASLAHPNIVNIYDTGFEDDIYYIVMEYVKGETLKNYIKRK
                     GSLSEKEAVKISRQVAEALKHAHSHNIVHRDIKPHNILLTEDGTAKVTDFGIARASTS
                     STINNTSNVIGSVHYFSPEQARGGYVDEKSDIYSLGIVMYEMVTGTLPFDADNHISVA
                     MKQIQEKPVPPSKRVKNVKISKNYEDIIMKCLEKHQSFRFQNTDELLKKLDALNGNNN
                     PVKDETEIIDSPTIEIPALNDLEENVINIDLIDENSDKAFKSFFSGNEEDENVEKSIN
                     NKKITIAAILSALVIAVIGGVILFKSFLYVPEVPVPSLLGRSEEEAKKLIEDLGLVFK
                     VSKREYSSEYDEGYVMEQSIDEGSKVKENYPIEVVISRGEKEIVVPNLIGKYAIEAGG
                     ILNNAGLSEGETTEENSDTYLAGQIIDQYPLPNTPARENDKVSYVVSIGPKIIYVKMP
                     NVLNLNLETAKLSIVQHGLAVGQVNEEPSEEIEKGLVMRQSITADQEVEQGTSIWLTV
                     SSGKPEEPDEPTPEEGVFPLAISLPADRDSVSIVIQRIASDGREVVYSEEVDTSEGNI
                     LVNVKGKGTGIFEIYIDNELYDRVEITFE"
     gene            9834..10712
                     /gene="rsgA"
                     /locus_tag="PLB33_00410"
     CDS             9834..10712
                     /gene="rsgA"
                     /locus_tag="PLB33_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG62011.1"
                     /translation="MIEGIITKGVGGNYYVDIGSKIIECRARGLFRLKNIKPLVGDKV
                     LIRITEEDEDMGYIEEIKERTSEIKRPPVANAQQLVIIFSVKNPEPSFILLDKLLIAA
                     KSNNLNPVICINKSDLANEEEKENILNIFKNTGYKIIFTSKYDEDSLQNLKDILKDRL
                     NVFSGPSGVGKSSLMNAIEPGFYLETGEISDKLKRGRHTTRHAEIFKLGFGGYAVDTP
                     GFSSFELEGIDEYSLKSYYPEIVKYDDGCKFLDCLHYKEPGCVIKEAVNSDLISRVRY
                     NNYIKLLEQIKESKPY"
     gene            10722..11378
                     /gene="rpe"
                     /locus_tag="PLB33_00415"
     CDS             10722..11378
                     /gene="rpe"
                     /locus_tag="PLB33_00415"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOG62012.1"
                     /translation="MKNKLAPSILSADFSNLGEAVKLVEEGGADYIHIDVMDGHYVPN
                     ITIGPAVVKDLRKTTSLIFDVHLMIENPDNFIEHFYKAGADIITVHQEATVHLHRTIQ
                     KIKSYGLKAGVSLNPATPISVLNEIIKDVDMVLLMSVNPGFGGQSLIENVKYKFYDLK
                     QLLNKYNLDIDIEIDGGVNYSNLKEILSWGANVIVAGSAIYHAEDIVLETKKFKEIMS
                     "
     gene            11375..12010
                     /locus_tag="PLB33_00420"
     CDS             11375..12010
                     /locus_tag="PLB33_00420"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOG62013.1"
                     /translation="MKTALIVGNGDETAKRIIENIKYDYLICADGGLEKVSNYGIIPD
                     IIIGDFDSVASNVLKQYENRIPIEKFPSEKDFTDMELAVELAVSKGYKNIILTGASGS
                     RLDHTLGNILLMEKYFKDGVNISIIDNNNEMKIISDNSELFIEYKEGYFISIIPITDS
                     IQGLCLEGFKYNLDNVDVERGSTLCISNQIKDNKGKITLKKGRAIVIISKD"
     gene            12022..13404
                     /gene="asnS"
                     /locus_tag="PLB33_00425"
     CDS             12022..13404
                     /gene="asnS"
                     /locus_tag="PLB33_00425"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOG62014.1"
                     /translation="MKTTDIKDIFLSPENYKEVRIEGWIRTIRDSKNFGFIELNDGTF
                     LKNVQVVFENNLSNFEEIKKFSTGSAITVRGNIVLTPEAKQPFEIKATEIALEAPSDS
                     SYPLQKKRHSMEYLRTIAHLRPRTNTFSAVFRVRSLAAYAIHKFFTERGFIYAHPPII
                     TASDAEGAGEMFRVTTLDLDNIEKVDGKTDYSKDFFGRSANLTVSGQLEAEIFALAFK
                     KTYTFGPTFRAENSNTARHAAEFWMIEPEIAFADINDNMDLAEDMVKYVVNYVMENAK
                     EELEFFNNFVEKGLLEKLDNVVNSEFGRISYTEAIDILMKNNDNFEYKVKWGTDIQTE
                     HERYLSEKIFKKPVFAVNYPKEIKAFYMRMNEDNKTVAAMDLLVPGIGELIGGSQREE
                     RLEFLEKRMDEMGVPKDNMWWYLELRKYGGVKHSGFGLGFERLIMYLTGMSNIRDVIP
                     FPRTPKNADF"
     gene            13506..13703
                     /locus_tag="PLB33_00430"
     CDS             13506..13703
                     /locus_tag="PLB33_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62015.1"
                     /translation="MNYFKHKKKCSPANKPSFFKFTEILGLMIATIGAIIIVQMLPVK
                     VWLFVLGILLIILGCTLFRLL"
     gene            complement(13820..14011)
                     /gene="rpmB"
                     /locus_tag="PLB33_00435"
     CDS             complement(13820..14011)
                     /gene="rpmB"
                     /locus_tag="PLB33_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOG62016.1"
                     /translation="MAKFCEVCGKGTISGHSITHSDRKINRTWKPNVRTITVMEDGRA
                     LRKTVCTRCIRAGKIQRAN"
     gene            14303..14662
                     /locus_tag="PLB33_00440"
     CDS             14303..14662
                     /locus_tag="PLB33_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOG62017.1"
                     /translation="MSVKIINENGSIYVDDQALAAIAGLAAMECYGVVGMASRNATQG
                     FFELLKREQLTKGIKVTSTEEDKINIDLYIVLQYGVKISVVAENVISRIKYSVETFSG
                     VSVENVNIFVQGVRVQK"
     gene            14676..16295
                     /locus_tag="PLB33_00445"
     CDS             14676..16295
                     /locus_tag="PLB33_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOG62018.1"
                     /translation="MLIKYIDNVKFKKMLLGAAVNLENNKTIVDSLNVFPVPDGDTGT
                     NMNLTLQAAIKEINNLSESTITSLASAAANGSLMGARGNSGVILSQLLRGMSKALKGL
                     NHIDCKIIAESLKSASDTAYKAVMKPVEGTMLTVARETAEKAMATYTDYEDVIEFIES
                     LIQQAKDTLNRTPEMLNVLKQADVVDAGGKGLIYLMEGALAALKGVEIKQEITEQAVI
                     DTEVELDEFTSEDEIIFAYCTEFLIKNPIKTAEEFLTRIYDKGDSIVCAGNENVIKIH
                     IHTNNPGQILAMAVKFGELSNIKIDNMKEQFRERLKENHKNNQKKKKYGFVSIGVGKG
                     IEKVFTDLNVDVFLSGGQTMNPSTEDILEAANDINAEHIIILPNNSNIILAATQAKKI
                     SDKNLYVVPSKTIPQGIAALIAFKEDSDIDENIEAMSQAVKEVKTGLVTYAVRDTVFN
                     DLEIKKDQIIAIYEGDITNHGENPEDEAVELINKMVDEDSFLVTVFYGDNVDEKTAES
                     IKPRIEEIAEDCDIEIIYGGQPLYYYIISVE"
     gene            16339..18393
                     /gene="recG"
                     /locus_tag="PLB33_00450"
     CDS             16339..18393
                     /gene="recG"
                     /locus_tag="PLB33_00450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG62019.1"
                     /translation="MLDADIQYLKGVGPNRALKLHKLDIFTVSDLLNYFPVKYEDRRT
                     VKNINEICEQGKYLLKVRIFEKPKYSRIKKNMSIIKAICKDDTGFVTLTFFNQDFLKD
                     KLIVGEYYYVFGNVKIAYGKLESTNPEIEFINKDWKAGKIMPVYGLTYGLSNNEITKL
                     VKTALETYYDEIQNVLPDEIIKKYKLISKKAAVKALHFPSDNKKYTLAKRTIAFEKLL
                     IMQLGLFNIKNNLTNELNDIELRNTELSEKLLSSIPYKLTKAQLKVIEEIKEDLKSQK
                     TMNRLVQGDVGSGKTVVAVYAMLMAAGSGYQSSMMAPTEILALQHYDTIKEYIKIAGL
                     DVKAEILTGSTKPKEKQEILDKLKGGEIDIIVGTHALIEENVEFKNIGLVVTDEQHRF
                     GVRQRARLSNKGKNPHILIMTATPIPRTLALMIYGDLDISLIDEMPPGRQKVITSIIS
                     EKEKTKAYDFIKSQVDSGRQAYIVAPLVDESENLDLNSAVEIYKELSTSHFSGYKLGL
                     LHGKMSNSEKEEIINKFYRGKINVLISTTVVEVGVNVPNAVVMLILNAERFGLAQLHQ
                     LRGRVGRGKCQSYCILVNESKSKKSKDRMNILKRTDNGFIIAEEDLKIRGPGDFFGVR
                     QHGLSKLEIQNIVNNVETVQEVQLLSKRILSENPYLKGSDYQNLKMEIMNLFKDETIV
                     FN"
     gene            18418..18981
                     /gene="rsmD"
                     /locus_tag="PLB33_00455"
     CDS             18418..18981
                     /gene="rsmD"
                     /locus_tag="PLB33_00455"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOG62020.1"
                     /translation="MRIISGTNKGKKLYAPEGKSVRPTGDKIKEAIFNIIGSIDEEST
                     VLELFAGSGSIGIEFLARGAKHCTFVDISRKSLNYVKKNLELCNFSDKAKIISSDYEK
                     AIINLSKDNEKFDYIFADPPYELNISNKILNKIVEYGILKSDGLLIIESDKSEKVIDN
                     EVANMIEYKEKIYGRTKISIIKYLEEQ"
     gene            18978..19457
                     /gene="coaD"
                     /locus_tag="PLB33_00460"
     CDS             18978..19457
                     /gene="coaD"
                     /locus_tag="PLB33_00460"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOG62021.1"
                     /translation="MKVLYTGSFDPITMGHLDIIKRISKKFEEVVVTVFNNKEKKYWF
                     SIEERCEMVREAVKDLENVEVDFTGGLVVDYCKRNNISIIVRGLRAVSDYEYELAISS
                     INKHLNNELETIFLVATSEYSFLSSSMVKDVAEHGGNIEGLVPDNVAQNIYRLLGRK"
     gene            19460..19903
                     /locus_tag="PLB33_00465"
     CDS             19460..19903
                     /locus_tag="PLB33_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOG62022.1"
                     /translation="MDILNLLERIEDIIEDSSKFPLSNKVMIDKEEVLEVINEIRLKL
                     PDEINRASWVAKERQRILNEAQSEADELINKVKEQQKHLIEESEITRQAKIYANEIIE
                     EAERKANEMKVGAYNYSDEILSKLQDKIKEINNIIEDNREVLKKM"
     gene            complement(19954..20589)
                     /locus_tag="PLB33_00470"
     CDS             complement(19954..20589)
                     /locus_tag="PLB33_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOG62023.1"
                     /translation="MPQEIYNASGIKIFGKRIKSLIYTTDLAIIKNNNADGVIAVYPF
                     TPQLAINQAIIDISPTPVFVGVGGGTTTGQRSIDIALNAELTGAYGVVLNAPTKNELI
                     SEMKERLDIPIIITIVSEKEDFESRIKAGVSIFNVSGGANTTNIVRQIREKHPYFPII
                     ATGGPTPESILETIRAGANIITYTPPPTGKIFSEMMEVYRHKITEELSTNK"
     gene            20834..21400
                     /locus_tag="PLB33_00475"
     CDS             20834..21400
                     /locus_tag="PLB33_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62024.1"
                     /translation="MKKLMLSGTVQIETRRFVLRKFQMSDAQDIYYNWLSDKDSAKYN
                     AWRVHTSIDETKEYLSEWTSLYKKDDYLHWAVVDKETKEVIGSVTVSNIKKWKKYCEV
                     GYTIAKKLWNQGIATEVLISILHYLTQEVGFEKVCAIHDIRNEASGRVMEKAGMKFVK
                     NKTHIFLNSDNIIMKCRLYEYRGMGTHL"
     gene            21550..22932
                     /gene="tig"
                     /locus_tag="PLB33_00480"
     CDS             21550..22932
                     /gene="tig"
                     /locus_tag="PLB33_00480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOG62025.1"
                     /translation="MSSKLIKKEKNTVTLEFTVSQDEFDKAVNKAYLKVKNNINVQGF
                     RKGKAPRHIIEKKYGKSIFYDDALDIAVQEAYPKAVNELELDIIDSPKINVEKFEDGE
                     EIVFTAEVEVMPEVELPKYKGIEVVKTEIKVTDEDVERELKSIQEKNARIVEVADRPV
                     QKGDFLTIDYAGFVGEEQFEGGTAENQTLEIGSNSFIPGFEDQLIGKSKGDEVTVNVT
                     FPDDYHAENLKGKDAVFNVKIHEIKAKELPEMDDEFAKDVSEFDTLEELIEDTRKVLE
                     KKAADQSKISNDNKVITKIVNDTKLEVPEVLIQREIEHMGRDYEQQFRQQGFTGKEYD
                     ELINNFVNQYKESARGQAEFNVKAELVLEEIIKKEEITVSDEELQEEINKIAESYKVE
                     EDRLEGFKESLMQSSKGYIEETLKKRKVFDMLVENAVFVDAPETVESIEAHETINESE
                     KAEEPEEDKE"
     gene            23002..23586
                     /gene="clpP"
                     /locus_tag="PLB33_00485"
     CDS             23002..23586
                     /gene="clpP"
                     /locus_tag="PLB33_00485"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOG62026.1"
                     /translation="MTLIPYVIEQTGRGERSYDIFSRLLKDRIVILSDEVNDATASLI
                     VAQMLFLESEDPDKDIQFYINSPGGSITAGMAIYDTMQLIKPDVSTICVGMAASMGAF
                     LLLSGAKGKRFALPNSEIMIHQPLGGMRGQASDIKIHADRIIKIRDTLNKIISEKTGQ
                     PIAKVQQDTDRDNFMTAEEAKIYGIIDDIIAKRK"
     gene            23598..24857
                     /gene="clpX"
                     /locus_tag="PLB33_00490"
     CDS             23598..24857
                     /gene="clpX"
                     /locus_tag="PLB33_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOG62027.1"
                     /translation="MSKVNEKHIKCSFCGKSQEQVRRLIAGPDVYICDECIELCYNIV
                     DEDIDLFEEYDFTELPKPKEMKAKLDEYVINQDNAKKALVVAVYNHYKRINYKTKDDG
                     VEIQKSNILLLGPTGSGKTYLAQTLAKLLNVPFAIADATSLTEAGYVGEDVENILLKL
                     LQAADFDVERAEKGIIYIDEVDKISRRSENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFIQIDTTNILFILGGAFDGIEKIIQRRVGKKSIGFGADISGNNSMDT
                     DELLKQVQSQDLLKYGMIPEFVGRIPVIVALEKLDKNALISILTEPKNSLVKQYKELF
                     KMDNVELEIEKDALEEIAKKALEINTGARGLRGILESTMTEVMYEIPSRSDIKKCVIT
                     KDTIINKSEPKLVLTKKPVSDKKENVS"
     gene            24897..27224
                     /gene="lon"
                     /locus_tag="PLB33_00495"
     CDS             24897..27224
                     /gene="lon"
                     /locus_tag="PLB33_00495"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOG62028.1"
                     /translation="MIKNYKIERRNLPLIPIRGIGIFPNTVIHFDIGREKSINALEEA
                     MLEDSDIFLTVQKVADMESPKEDDFYEVGVICKIKQMIKMPGDNIRVLVEGINRAKIV
                     SVTQDDPYFEVALDEYIYDDSLPIDDNLEAMVRLTLNNFEEYALIYAKIAPDAVISLK
                     EIKNPDKLADVVASYIYLKTNQKQALLEIFDPYKRLESINKMLAKEIKVLQIENEINE
                     LVKKQVSEFQKDYYLKEQMRAIQKELGEDEGTDYEVEEYLQKISKANMPKDVKEKAEK
                     EINRLLKISPSSPDAGVIRTYVDWLLDLPWNKKTKDNTDLSRARKILAEDHYGLEEVK
                     ERILEYLAVKNIKKDMKGPILCFVGPPGVGKTSIAKSIARAMDRKYVRISLGGVRDEA
                     EIRGHRRTYIGAIPGRIISSIAKVKVNNPVFLLDEIDKLNSDFKGDPASALLEALDPE
                     QNNTFTDHYIEAPFSLQNVMFITTANTTSTIPGPLLDRMEVIEISGYTDIEKRNIAEK
                     YLIKKQLSEHGLKENQVKISTNAINEIIERYTRESGVRNLERNIGKLIRKAAVQIVEK
                     NKSTVSINNLNIKKYLGIPKYDYDIAYEEDQVGVVTGLAWTQVGGETLFVEVNTMKGD
                     GKLQLTGQLGDVMKESAMAALSYIKANAENFDIDNKIFKETDIHVHVPEGAIPKDGPS
                     AGITMATAIISALTKKPVRKDVAMTGEITITGRVLPIGGLKEKLLAAKRAGIKKVLLC
                     SKNKADVSKIDPKIIRSMEIVYADKIDDVVKEALR"
     gene            27275..27868
                     /gene="yihA"
                     /locus_tag="PLB33_00500"
     CDS             27275..27868
                     /gene="yihA"
                     /locus_tag="PLB33_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOG62029.1"
                     /translation="MIIRKAEFLISAVKKEQYPNTGVPEIAFAGKSNVGKSSMINALV
                     NRRNLAKTSSQPGKTQTLNFYNINDEFNFVDLPGYGYAKVSKAEQAKWADMIDLYLHS
                     RQQLKEVILLVDIRHEPGRNDLLMYEWIKSFGFKGYIIATKADKLSKSQQIKSLNNIK
                     KTLQIKDIGLIYQFSSTSKLGVEEIWILFESILNLHD"
     gene            27967..28353
                     /locus_tag="PLB33_00505"
     CDS             27967..28353
                     /locus_tag="PLB33_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62030.1"
                     /translation="MHYVVERKVNSDIKCGKKIIEEILGSIEGLIDPNTYFNTKLILN
                     ELMINSVVHGNCGDLNKMIYIKVYINESRIIVEVSDEGSGICYEHKNCGEYDYLECGR
                     GLMLVEGLSDKLSIEGNTVTCVQYLK"
     gene            28452..30752
                     /locus_tag="PLB33_00510"
     CDS             28452..30752
                     /locus_tag="PLB33_00510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOG62031.1"
                     /translation="MDNIKLSVRELVEFIYKSGDINVRNLGADRAIEGIKAHKILQNQ
                     MGENYYKEYYLKGEFVLNDIIFLIEGRADGILIEDDETIIDEIKSTYTDLSLINGEYS
                     TAHMAQAKCYGYMYGLSNNLENISVQLRYYNLDTKEVKTIKQTFSTEELKIFFYSLLN
                     TYIDWAETIINLRKERNRTIKELNFPFEKYRKGQRDFSIAVYLSIRSGKKLFAQAPTG
                     VGKTVSVLFPAIKSLYEKNAKIFYLTAKSSTKTIAFNTVKMMHKQGLRLRTTVITAKD
                     KICFMDETKCEPEYCPYAKGYYDKLNIPLRETIKNNCMYDREFIEELGKKYELCPFEL
                     SLDLSYMSDIVICDYNYYFDPRVALKREDLFNKDKDILLIDEAHNLEDRVRNMYSPEL
                     IKEEFYEVYKIIKSEKSLSKELFNINKKFINIKKSIDEAQILKETPEELIKALRKFIA
                     KADKYFNERKNEKIPEELTDIYFKSIFFIKISEIADDNFCYYADSANNKTMVKLFLID
                     TSQILKEIERNARSSIFFSATLTPLKYFRYILGGEEDDYILKLSSPYDKENLNLMITS
                     DISMKYTKRDSNIEKTCEYINTLITEKKGNYMIFFPSYNFMNKVYSVYESLYDTMNII
                     VQSQGLNEEEQMDILSRFENERNVVLFTVVGGTFSEGIDLPLDKLIGAVIIGTGIPQI
                     SFERNIIKAFFDEKMNTGYDYAYKYPGFNKVLQSAGRVIRTEDDRGTVLLIDSRFCQY
                     TYLKLFPDHWKHFKKIKTSEDIKNLL"
     gene            30766..31575
                     /locus_tag="PLB33_00515"
     CDS             30766..31575
                     /locus_tag="PLB33_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOG62032.1"
                     /translation="MNILSDKNVMNNQIITKEETELLINKTKIYEVVRVIDGKPIFLK
                     EHFQRMSESINLSGIEGNINYEEFKNSVELLIKENSFKNLNIRVSYFYDKKPLTLFYF
                     IESYYPSKDEFAEGVHTVTVKMERANPNVKSFQKEYKDKVTRIIKENNAFEAILINHD
                     NTVSEGSKSNIFFVEGNKLITSPDSSVLLGVTRSKVISVCEENSIEVHKRIIRLDEIE
                     SFDGVFLTGTSNDVLPIKTVDNIVFNSSENEVVKKVSRLYLGEVKKEIEKG"
     gene            31848..32990
                     /locus_tag="PLB33_00520"
     CDS             31848..32990
                     /locus_tag="PLB33_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOG62033.1"
                     /translation="MLVYLDNSATTKVRDEVLKEIIDAHENYYGNPSSLHRMGHKVEK
                     KIDEARTLSAKLIGAKPEEIYFTGGGTESNNIAIFGHLSNLKEYNNVITTNIEHPSVY
                     NVFKQLEDRCLVKYLETDENGKINIDELHNMVDENTLLISIMHVNNEIGAVQELNEIT
                     AIIKRKNKNAKLHVDAVQSYGKIKIDVNKTPLDTLSFSSHKIHGPKGVGGLFIRSGIK
                     ITPLIYGGGQERGIRSGTENTTGIIGFGKACKLMYDNFDEEVNKLYGLKNTYIKRLEE
                     IEDIKINSPIDGAPHIISVSFKNVPGEVLVHYLEDKNIFVSTGSACSSKSKDSRILEA
                     IKLSKEYRAGTIRISLGHFNNFEEVDYVIDNIKKSVKDIRSITKRG"
     gene            32992..34152
                     /gene="thiI"
                     /locus_tag="PLB33_00525"
     CDS             32992..34152
                     /gene="thiI"
                     /locus_tag="PLB33_00525"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOG62034.1"
                     /translation="MYNIAAVSFGELFLKGKNRGKFEQKLIEQIKYALKEFNHVSVYK
                     DSGKVYVETKNEEDMDKIVEKVRKIFGISNISPSVKTSKDVDIIIEKTIELFRYLLSK
                     EDIKTFKIKTKRSDKEFPLKSMDFSALVGGKILENFSFVKVDVHTPDVEVYIDIKKCC
                     YISSERIKTLGGLPLGSNGRALLLLSGGIDSPVAGYMIAKRGVEIDALYFHTYPFTSE
                     RANEKVIKLKQKLEEYCGKIKLYSINMLEIHKAIREFCKEEETTILARRFMMRIAEKI
                     ALENKIDMLITGESLGQVASQTMKSMAVIEDAINMPILKPLVGLDKSEIIEIAREIGT
                     YETSILPYDDCCSVFAPQHPLINPKLESIKRSESNLNVEELIEKVLLTLEIL"
     gene            34389..34529
                     /locus_tag="PLB33_00530"
     CDS             34389..34529
                     /locus_tag="PLB33_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62035.1"
                     /translation="MSDEVRATNFRCCDDRSNDWFEWLIILGIIFFLLGGNNFFGRGF
                     GR"
     gene            34634..35815
                     /locus_tag="PLB33_00535"
     CDS             34634..35815
                     /locus_tag="PLB33_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HOG62036.1"
                     /translation="MFKVALFCDIPQETLNLLPEDKYYLIREETSEADGIIIRSLDIH
                     DMKFSKQLKAIARSGAGVNNIPVERCSEEGIVVFNAPGANANAVCELTFLGLLMSSRK
                     VIDSIDWTKKLESNVVETVEREKSKFVGPELKGKTLGVIGLGNVGHLVANLGTKLGME
                     VIGYDPYISVKNALSLSWDVQYTGDINDLYKRSDYITIHVPYNNETKDYINKDNIKLM
                     KDGVKILNFARHGIINEDDLIEALESGKVSYHVSDLPNEKLLKAKNTLCIPHLGGSTI
                     EAEENCAEMAVTELTEYLEKGNITNSVNFPDCAMDRYPGTDRIVVLNKNIPNMIGKMT
                     TALASQNVNIIGMVNKSKGDYACNIIDVIGELNEDMLNGLVEHYGILRVMLIKNKRSK
                     K"
     gene            35812..36255
                     /gene="rnhA"
                     /locus_tag="PLB33_00540"
     CDS             35812..36255
                     /gene="rnhA"
                     /locus_tag="PLB33_00540"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOG62037.1"
                     /translation="MKKVNIYTDGACSKNPGPGGYGVILEYKGHEVELSGGSENTTNN
                     QMELMGVIIGLEALKEPCDVTIVTDSKYIADSFNKGWIDAWQKKSWKKSDGKAVLNKE
                     LWQRLLKAMEGHKVKFVHVPGHKGHIYNERCDKLAVQERKKFEKK"
     gene            36330..37037
                     /locus_tag="PLB33_00545"
     CDS             36330..37037
                     /locus_tag="PLB33_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG62038.1"
                     /translation="MIYRQNQQLCPNIYSMPYEVRPGDTLNSIAARFGTNLVGLIELN
                     PTLRLRSLTEGMTICVPRVPERPPCVNGAYYTIREGDSFYNIAQRYGIPLNLLLEANP
                     EANPYDLKVGQEICIPDVPPGCPFGTVVTIQEGTRLSDILISYNLSLNELRDANVNFN
                     PNIIVPGTELCIPQETFMQCPEGTTTEYVIQAGDSLSTVAIANNITPSELLIANPHLR
                     PANFLIVGTRVCIPTAR"
     gene            37160..38050
                     /locus_tag="PLB33_00550"
     CDS             37160..38050
                     /locus_tag="PLB33_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOG62039.1"
                     /translation="MYVEKIVTTGICQGKLFLFIMELLKEDYSFKKALYLGAGDSFAI
                     HSIEVFNDAVYMTDSFNNKIYKFDSVNNFFETNVGRDPRHMCTDKDNIYVANFESDNI
                     SIIEMDTFTLTGSLPAGIKAHDVIYSEKNKSLYTTCYEENEVLEYSIDNGIRRRFKTD
                     GKPMHIFVLDDTIIVMTYYVNGIVQTKINFINLSNKEIEDTIDIDGLASDFLLDYENN
                     MLYIINIVDKSLYIADSLLRKIIKKIYLGGYPESISCGSKNIYVTNSKKQQIDIIEKQ
                     GHNISTLKLMFTPGLIKIIP"
     assembly_gap    38105..38204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            38568..39656
                     /locus_tag="PLB33_00555"
     CDS             38568..39656
                     /locus_tag="PLB33_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG62040.1"
                     /translation="MKKIIILIALIMMTGCGAPTAEIPENPPAVEAPGEEYPEEDKED
                     TDNLNEDKELSDEEILASIDLSLNPNELGEIMILMYHGIGPEESVWQRTPDNFRKDLE
                     YMYQNGYRMISLNDYAKGEIKTEAGYTPIILTFDDGRQNNFSYIEENGKIVIDPDCAI
                     GILEEFKNKYPDFNVTASFFLGTNLFGQKEYAEKKLNWLIENGYDLGNHTYSHNEMAE
                     LNSDEIIIEIGTVNNIIKQYLPDYAVETLALPHGSNPKDEFIESVLEGEYEGNKYRTL
                     AVLDVGWRPAYSPFDILTNFSNLYRVTASEINVDNCGINDYFKQYEENKRERFISDGN
                     PDVVTIPKRHVDYLNMDIVGDRIVNIYE"
     gene            39832..40536
                     /locus_tag="PLB33_00560"
     CDS             39832..40536
                     /locus_tag="PLB33_00560"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOG62041.1"
                     /translation="MIAELKEIISKSDNIVFFGGAGVSTESGIPDFRSVDGLYSKKYK
                     YPPEMMVSHSFFRSNTEEFFDFYINKMIFLNAKPNSAHKKLAELEKEGKVKAVITQNI
                     DGLHQAAGSKNVLELHGSVHRNYCMKCRKFYDVNFIVNSKGIPKCQCGGIVKPDVVLY
                     EESLNTDVIEKAVNFIERADVLIVGGTSLVVYPAAGLINYFKGSKLVLINKSSTPMDR
                     RADLVINDSIGKVFED"
     regulatory      40590..40706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            40807..41961
                     /locus_tag="PLB33_00565"
     CDS             40807..41961
                     /locus_tag="PLB33_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62042.1"
                     /translation="MKKFKVIAMVLAIVMILSQSLVFAEDYAIEIGGTLVATELKLTL
                     EDLKSMPEEAQIAQEYVYNSKGGEKTVQVKGVSLAYLLKEMAGVTAEEGNVEFFAADE
                     WPIDPQPLQDVLNDELKFVLAYEVDGEAINNDEGIIEEITVYRNLKYEDEFDTVYKLI
                     NKITVGEAEAVEEEEAPEEETIEPIEEETEETEETEEVTFTDITEEYKFAEAAIYDLA
                     KRGIIDGMGGGLYAPGNEFTREQFCKIIVVALEYELKEYEGAFSDIDDDRWSAPYVQA
                     AVDSGLFVGYPDGTFLPEKVITRQEMALVAARAAVAKGLVEQAKVDKFVMEKSDYIDK
                     EDVADWAGNAVAWLEAQDVFAGIAVEKFEPARNVNRAEAALVVFNTLFSE"
     gene            42013..44844
                     /locus_tag="PLB33_00570"
     CDS             42013..44844
                     /locus_tag="PLB33_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62043.1"
                     /translation="MTRKITVWFLIVSMIIMNATFVFAAEPELIITGTGVEEEVVISS
                     GDWSKYKMVERIYSTNNSLGFHKIIKAKGYDLFELMGNGLKTDKDYSVKFTCSDGFEF
                     TKTISELKSAYYFSDFTLESKQAVMPMVAKYTTVMADFPKNIFAPPLTWTDIPITEKD
                     LDKDFPKLVFGQTDIDDMNMSKWGKEVIKITVGDEIVKVTPSSPYKHISYEGEPYNVD
                     AVTGATFTIEGPAVEGYRAISLRQIEEDTTGQTIDTYYEKVNGKVVEVSYEGINVKYL
                     IDNYVKAKSNAGNIIFKDKSRKTIITADISEAGNYMVAYGINEVPLVYLDTDEGYIKE
                     KHNDNGCFKLVYEQDKNKAEEFSNVAYIYIEEKDAKNIFEHSYAPYNAEKYTDYELII
                     HGSALGKEVRYKVKDIEAMTDLHETHEYSLSNSEYFWYYNTYKGVPLWDLLLKAGLDP
                     DIDENTSVRFIAADNYNFAPMTIKEIKDRSLYGYYEKSAMDKGDGTFPGEYVDPLYTN
                     LPVMVAYGFNKYPYVIRPTDEGYNPGLGNDGGPLRIIFGKTNYNDTNGSNQVQFAKEI
                     IIGEGESLATDTQGIGEGVSTEIEIDESWNHNQGVYKEYLDMPVLRVTGSQVKQPMTF
                     TLRQIEAMTEYAVRDTYTGDGIREMQGIVLWDLINNVVGLKEDVEVPSIRVFSGQNYN
                     QILRSNEQVVNGVLNSQGQTKKIILAYAADGYPLVPDESSIGYVNNNAYGPLRLIIEE
                     SKSMWVKWTDCIVVGTGDYEAPDMKDVKIDTFSDIESHWAKDSIIEMAAMGYAKGSDG
                     LFRPNDNISRAEFVSLLIRVLGMGNEDAIPKFNDVKATDWFAKDVAIAAEKGYVKGYE
                     DGTFKPNSPISRQEIGTIIGSLLEDDITNAHEILSIFSDEVPEWAKVPVAKTVKAEII
                     KGLPGNIFGGAQNTTRAEAAVMLLRYLDK"
     gene            44998..45768
                     /locus_tag="PLB33_00575"
     CDS             44998..45768
                     /locus_tag="PLB33_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG62044.1"
                     /translation="MKKLLIILFAILLISAGCSQNAADAPATNEITESGENNNEVSKE
                     IGNSNEPEAEEDEKTEEPLSEKPSEEAEEAAKKEEPSEEISEQKEPITEEVLEDNPAE
                     EETEITENALIIKGHAEKELSLTLDELKAMDDIIFEADFYSLNNFGTTGYTHFKGVKL
                     WDLLKNEALIKPEASKISIIAQDGYSMEFTIEQVQMEYMDETNPENKYPMIIAWEENG
                     EEYSLDEGNPYKLVVGQKEPGDINKPQWVSNIDVILIE"
     gene            45777..47336
                     /locus_tag="PLB33_00580"
     CDS             45777..47336
                     /locus_tag="PLB33_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG62045.1"
                     /translation="MKNRYIIIIFIILVIAGSLFYVLNDSSEEEEAVRLFYPDAKRVT
                     LIKGINDDLYSSLYFPAVKRAYEVDGEISAYVVSCVGYNGPVEVLAAIDDDKLIGIKI
                     LSHEESLDYAEHIEYDYFLDRFKNLPINKYLNLVVLDKENPEDIIQVTGATISSQAVV
                     NAVNAAIGSYMLWNYDIQMAKVPDVVPQEMWQKDINSFAINWEGGSIRIDTDEIKEYE
                     QLEMDVTLINTTGTETKMRVKGPTLRHVLEKEGIDLSEYAGIGVTGRDGYYTLIDKEK
                     LAVNDIILTWQVNRKNIKDEEKPIRISVPLELGPYWVKMVSNIDLYKEISPKDIDKVH
                     MFNPLTEDIEPYYYEYYGSKDKSIEVGKILRKFDVVDEKGFFTMAATDGLEKHETISL
                     VRQRYFLKVEGDNAPMNIAPNFKLGMNVKHMTHFSTTKDAVIFPEKMIEVVRTKSIEG
                     NDGMLLEDVLLTAGMRWDEGNKFTAVNKNKKIDLSLEEMLNCYLIYKEGQVSLYNDKE
                     IMTELSRIEKK"
     gene            47333..48490
                     /locus_tag="PLB33_00585"
     CDS             47333..48490
                     /locus_tag="PLB33_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG62046.1"
                     /translation="MKLSIRSIIQITATLIVIISCLIYGMYINEIIEFRILSIADLNP
                     YGGWSALKSLFTDLSYRWKGFSRSISLTLGIVAASFLMGRFFCGYICPIGAMQDFFKY
                     IGRKLRFKEVRFSNKPEILKYIFLIVIMVLSIVGMGNIVSPLSPWLAYLNIFAGLKLQ
                     TGAVILLFLIFVSLFGRRIFCRYFCPLGAFQSLIYAIGPLKIKKGSCNCSYCLKNCPV
                     SEEFRRTENEISPECINCLKCINSCIKNKEGFSITFMNKKINRLTYIVISIFIIAGSY
                     ILLPLTVSNSSVQAITSITGINDGTYEGFGFGFGGLINTEVTINNNKITDIRILNHSE
                     TAGYYEEVFRELAYEITETQNLNLDAISGATSTCRGFLNSLKDAVNKSLAD"
     gene            48499..48969
                     /locus_tag="PLB33_00590"
     CDS             48499..48969
                     /locus_tag="PLB33_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62047.1"
                     /translation="MQKKFIILIILIIVLSGCSKEKPSLNLNIDGKVESINTEIIYTH
                     KEALVFPAVVRSSGSKPVETEYKGIELIKLFDSLNVDITSYSKVTFNASDGYRIILSM
                     EEIKEPSNVYLTFERDGEILKSKKQGGNGPFQLVIRRDPFSQRWIKHVNEIILE"
     gene            48976..49527
                     /locus_tag="PLB33_00595"
     CDS             48976..49527
                     /locus_tag="PLB33_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOG62048.1"
                     /translation="MTNKFLEKFSVYNLIIIAVVSALGIATKPIVVPLVHIITGPLFI
                     PGGAIAGGFYMFWIVLGAGIVKKSGTGTLIGIVQGILVIATGTMGTHGIMSIVSYTLP
                     GIVTDIIFMFSINKKYNILHYVFGCLAANVTGTLISNILFFRLPFVTVILILSAAALS
                     GATGGLIAYAINRSLEKMKILED"
     gene            49530..50570
                     /locus_tag="PLB33_00600"
     CDS             49530..50570
                     /locus_tag="PLB33_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOG62049.1"
                     /translation="MKLKKILSAINYTEHSLGIKLFLLIFLVILVGCSADVNYKESTG
                     INIFNENKNFFVDFKSEKEISINEITNDKFSEAAVILNTGEIIQVPAEFYIKNNTVTA
                     DGETVENTVGIYLTDNYQSITNAYYDMKEYLDKGEKVMTVLLDGFSYNQFKRANEENT
                     LTFLSKYFQYPALSVYTPVTNAGFAAIITGQAPDVNGVHDRSNRRMKVDSIFKYALDN
                     NKSCILLEGDIKILNTEIEPELHTDINKNSETDDEIYESALISVNEDYDLIFIHFHGI
                     DDRGHSFGPYSREVMDYIKTIDKYIKDLSEEWEGAIILVPDHGMHETAEGGGHGICRQ
                     SDMVVPYFIKEP"
     gene            50567..51079
                     /locus_tag="PLB33_00605"
     CDS             50567..51079
                     /locus_tag="PLB33_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62050.1"
                     /translation="MKKRALIIVLLVIILTITLIISLNLNKASIEKQKQILSNAQIVL
                     KYNDKEKVLTKEEIILYGEDFEAVLDTSTTEPSTHIYRGVQLKDLLNNHNIDLQDKII
                     IIKAADGYSVAYSSEEVSIDKNVYIAFMEDGRYLGSRDSGGRGPYESIVVSDFFSNRR
                     CKWITEIEVK"
     gene            51076..51732
                     /locus_tag="PLB33_00610"
     CDS             51076..51732
                     /locus_tag="PLB33_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62051.1"
                     /translation="MMNAVEVKNLCFKYDNRKEYIFKDVNFNVRTGETIAITGRSGSG
                     KTTLCHCICGLVPRLYKGELTGEVLIFGENIKNLSIAEVVTKIGIIFQNPSTQLFSPT
                     IEDELAFGPENLCVEREKISSRIDEILRIIKMEKYRYDNPNNLSGGQQQLIAIASVLM
                     LKPSILICDEIMSWIDDEGRKIIKDLLLKLKKEGKTIILVDHDNDNLNIADRIIHIGE
                     "
     gene            51732..52385
                     /locus_tag="PLB33_00615"
     CDS             51732..52385
                     /locus_tag="PLB33_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62052.1"
                     /translation="MNNKIEINNISYEYPRGRKVFINFSLELNKGETTVITGKNGCGK
                     TTLAKLIMGILKPSFGEIKLFGQSTKDMSLGAIGEIIGYVFQSPERQLFGTTVLEELT
                     FPLLFKGYNREETMDRAEKMLEIFDLKKVRNSYPYFLSYGEKRCLAIASVLMVGPEYL
                     ILDEPTASLDKERIEALSQALENLKKRNIGMLIISHNKDFIERHKDRIINLEGGSYE"
     gene            52378..53112
                     /locus_tag="PLB33_00620"
     CDS             52378..53112
                     /locus_tag="PLB33_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOG62053.1"
                     /translation="MNNIDPRTKLIQVLILSTLALIYNEITLLSIILIAAIMIALINN
                     VNFISVIIRLNKLLKIIFLIALIQSLLTNMGNPIFKMGNFTLFTDYGILKSFEFILRM
                     GIIIVSAAIITTSSSREIIQGLIQWNCPYEIAFMVSVAVRFLPIFKEEMTDIITAIQL
                     RGIDLKKVKLSKKFQVYKYILTPITVNSIMKAKELAAAMEMRGFRAYSERTSYLVLKM
                     QKFDYILLGLSISAALLFIIITRGAL"
     gene            53109..53855
                     /locus_tag="PLB33_00625"
     CDS             53109..53855
                     /locus_tag="PLB33_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOG62054.1"
                     /translation="MKVFSVIGLTKSGKTTTIENIIKELRKRNYTVGSVKEIHFEKFK
                     MDVDGTNTHRHKMAGSQLVTARGAYETDVLFQRKLSMNEILCFYNHDFVILEGVRDTC
                     APKIVTAHDVEGIIERLDETTFAISGQISNSLTEYEGIPVINSLTEIEKLVDLIEKKV
                     FDRLPDMKDECCKKCGYTCTQLCSKILKCEAERRDCVLAENSVTLKINGQEIAMNPFV
                     QNILKNTVEAIAKELYGYTESGDIEVCIKR"
     gene            53840..54460
                     /locus_tag="PLB33_00630"
     CDS             53840..54460
                     /locus_tag="PLB33_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOG62055.1"
                     /translation="MHKEIKLRSKRNNVFRIVEDEGTYILKKFENHENYIREKEVLSI
                     LKKAGVNVPSIIKAEENYLYLEDLGEVNFLEWYEEQEKNNALNISMVYELCSWFKDFY
                     SAVFEFYKKQFILYDVNFKNFIICDNKVYGIDFEQVKPGHIEEDAGRLSAFALTYNPS
                     MTEWKMDFRNILINILSNELNIEKEKIISEENKELAAIKKRRGAFS"
     gene            54468..>54764
                     /locus_tag="PLB33_00635"
     CDS             54468..>54764
                     /locus_tag="PLB33_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62056.1"
                     /translation="MKSLSILIIILIISIVGCSSDKEIIEDINEEVLEISKDGISEDE
                     QTIEYSEEIHEENQQIPEEEEPLPELSAKEPLYDEESENDMTSAPADEPLAELEQ"
BASE COUNT        20490 a         7509 c        11061 g        15604 t
ORIGIN      
        1 ttttttatct tttgataagg ttttattaat gctatttcaa cttgcttttc ttgccttagg
       61 agaagaaatt gcatggcgtg ccttctttca aaagcagttg aacaagactt tttctattat
      121 acacgtacta ctaatttcat ctctattatt tgccattgga cattttaatc agggaaatcc
      181 tgtcattgta atatatgata ttttctttgt ctttataaac agtattttat atggaattat
      241 attttacaaa tcaaagaatg cttgggttag tgcaatatcg cattttgctg caaatctatt
      301 tagtgttata gtattagtat ttatataagc catactcttg atgggcagga gtatttcttt
      361 aatcaaggag gtgactttaa ctatgataaa ttttaataaa ctacccttga tgatcgtcat
      421 tgcagtgatg gcagtcgtta tattaagtgc atgtgatggg gttaatagta atagcaatga
      481 gacggatgat attatcactg acgaaaatac acccactacg gaaacaactg ctgttccgtt
      541 cccatggggc ggcaatattg acgggaaaga aatgacactt gacgatgtac accagcttgc
      601 cggaaaaggc gatgatttat tgtttgaaga tttatggcaa tataagggag gaaataccag
      661 ttcaagtcat gaacgataca ttatggttta taaagtagaa ggaggatatc gtttagttgt
      721 acattcagat tcaaccggca agccggatat ggtaaacctt gaaagtattt gggaaagtgg
      781 cggtagcggt attgatatac gatataatga tgtgtatgaa ttcctgcgcg ccaatccttc
      841 acaagatggc atcacggagg aagaagcaca ggatataggg caaacaaagc ttacaatgga
      901 gttggaacca gtaagctggt atattcttgg cgattggccg gaaagcacag acgagaatgc
      961 tgtttttgca gaagcattgc tggattcaat ttatacaata gatgaaccat gttgggtctt
     1021 tcgcgtgaaa ggtacagccg agtggggagg cagttattat gctgtgggga aaaagagcgg
     1081 aactatattt atatgcagct ttgatgataa cagtaaaccc atatggagtg ttgtggattc
     1141 tgaatcaaat tcttaaaaac agtatttatt tctgttgaat aattgaataa taaaagatag
     1201 aaggacaaaa aatatcatat taatctgtct tcatatctta atgaaataca tcatgttaca
     1261 gtcaatgatg aaagtggacg ggaagctcgg ctttgcgatt atgattgggt attggatatt
     1321 cgtgagcaat ataagaaata tgatattaca ttttggttta aaggcacagg gtcacttttc
     1381 aaacatgacg gaacaatcaa gaaaataaat ccgtttaaac aaggaagtca tgcaaaaaaa
     1441 tttgatataa acattaagaa ctctggggac cgagcttgaa atataaattt tgtgattaat
     1501 ataactgcaa aataatgcga ggaagacata tggataattt agaacgaatt gctcaatttg
     1561 agaaattcag ccccatgttg tatttagact ataaattaac gtcatgggga cgaatgcttt
     1621 gtgacatagg gaataaagaa agctatacat ctaattatgc tgtaatttac agaaatgaaa
     1681 atgttgacaa tataattaaa gaagtagaag aatactataa ttcaagaagc ataattccaa
     1741 aaatatttaa tcgctgcggc tcggtggaac tggatatatt aagaccattt ttcacaaagc
     1801 atgactatgc aataagagaa tttgacatgg atttgatgat tttagataca catagtacca
     1861 gaaataaaat tgcagaaaaa gttgaggtaa aaataataaa ccgtgctctt gaaggtcagg
     1921 aatataattt agcaatagaa caagataatg gtgaaacata tggtgtaaag cttttaaaca
     1981 aacaaattgc ttcaggaaac aatatgtttt ttgcttataa tgaattaaag aagcctgtaa
     2041 gcatggcatt agctgaaaaa tataatgatg ttgtctacat aacagatgtt tatacaacac
     2101 cgtcacagag gtgcaatgga tatggaacgg ctgtcgtata tgctgtttta tcacattatc
     2161 ataattcatt gatttacttg catacagaca acccggatgc agcaagtatt tatagaagat
     2221 tgggattttc aggcaagaga ctaaagtcat ggtgggctgt aaaaggttca ttgccggagt
     2281 ggtgtttgga ataatatcta cagaaagtag atatgagttt gtaggagatt caatggaaaa
     2341 acttcaagat gattacaatt taataacagg cgtctatata ggtgatgtat tggatgtcca
     2401 tttgtaatga ttgaaaatta aggaaacttg aaaattagga gaaaagtatg ttagacagta
     2461 aaggttttga cttatgggca catggttatg ataagagtgt aaaccttagt gaagaaaaca
     2521 atgaatatcc atttgccggc tacaaagatg tattaaatta tatttataac caagttagga
     2581 aacataatta ttcaagcgtt ttggatatcg gatttggaac cggaatatta acaacacaac
     2641 tatataatga aggttatcat attacaggaa ttgatttttc acgcaccatg attgatatag
     2701 ctaaaagcga aatgccggag gccatattaa taaattggga ttttacgaga gggctacctg
     2761 tagaaattaa aaacaataaa tacgattata taataagcac atatgcaatt catcatttac
     2821 cggatgaaga aaaaattaaa tttattaaat cacttttaaa actgttaaat gttaatggta
     2881 aaatattaat tggtgatgtt tctttcatga caaggaaaga attagaagaa tgtaaggaaa
     2941 cgcatacaga aatttgggat gatgaagaat tttattttgt tgcaaatgaa atgatgatga
     3001 gtctaaatga attttattgt aattatgaaa agatatcgta ttgctcaggt atattaatag
     3061 ttgcggatta tctgttataa ttagtaagag gaaaatttga attattaagt taaatactta
     3121 aaaaaattct gtaggagaaa ttgtatatga aaattttaaa aaagcctaac aggttaaacc
     3181 gtggagataa aatagcatta gtaagcttgt cttggggcgg tgcaggtgat gaaaatttat
     3241 tgtggcggta taaacaagga aaagagcggc ttgagcaagt tttcggcttg gaagttgttg
     3301 aaatgaagca tacattggcg ggaacagaat acatatacaa tcatcctgag aagcgagcag
     3361 aagatttaat gaatgcgttc caggataatt ccattaaggg tattattgca tgtatcggcg
     3421 gaattgatag tatacgaatg cttccattta ttgattttga tataatcagt aataatccaa
     3481 agatttttat gggatattcc gatacaacaa caacgcatct tttctgttac aaagcgggga
     3541 tatcgagtat ttatggaccg acactattgg ttgattttgc agaaaatgtt tcaatgagtc
     3601 catatacagt tgaacatata gaaaaaactt tgtttaaatc agaggcaata ggagaaattg
     3661 agccttcacc aaagtggacc agtgaatatc ttccctggga gggaaaaaac aaattaattc
     3721 aaaggaaatt taaagataac agcggatatg aactcttgca agggaaggga attacaaagg
     3781 ggcgattaat cggaggctgt attgaagtat tcgattcgct tcgtggtact gaagtctttc
     3841 caaagttaga ggattttgat gatagtatac tgtttttaga gacctctgaa gaaaaaccgc
     3901 ctgcgtggat tattgaatgc agtttaagaa tttatggaat aaatggtata ttggatagaa
     3961 taaaaggaat gatatggggt aaaccgcagg atgaaacata ttatgcggaa tacaaagaag
     4021 ctataaaaaa agttatgaag gaattcggga aagaggattt acctatttta tataatatga
     4081 acttcggtca taccgagcct aaaatatgtc tgccgtatgg agctatggcg gaaattaatt
     4141 gtgataaatc atcattttcc attttagaat cagcagtgct ctaaggcaaa aaaagaaaaa
     4201 gaagaatttt atggagtagc tatggagata aaagtaataa aaataaataa tgtaagaatt
     4261 gctaaaattt acagtaatga aattttaata aaggatggtc agtcagcctt ggatttattg
     4321 gcaacagttt catatgaaac agattgcgat cgtataataa tcgataaatc tgcaatagca
     4381 gaagattttt ttgacttaag caccaaatta gccggagaag tgttgcaaaa atgtattaac
     4441 tttcatttta aggtcgcaat cataggtgac ttttccgtat ataaaagcaa tagtttaaaa
     4501 gattttatat atgagtgcaa taagggaaaa gatatattct ttttgcccga tgaaaatcag
     4561 gctgtagaaa agttaagcat tgtacgaaga tagagtagta cctcgtcccc ttaacgcgtg
     4621 tattcttgat ataatataaa aagatgattg aaagaataaa ttggcaagat gacagtaccc
     4681 ccgtcccctt gtcatctaaa aagaggaatt atgaaaagtt acagagcgca agcaattgaa
     4741 acactgaaga aaatatttaa agataaatcc tattcaaaca ttgtgataaa taatgatatg
     4801 aaaaatgttg ataataggtt cctttccttg taccgaaaat cggttttggg agttgttgaa
     4861 aatctaatat ttattgattg gattattaat caggtatcca aaacaaaaac aaagaaaatg
     4921 gaaaccgatg tcttgcttgt gctgaggtta gcagtatatc aaatattctt tttggataag
     4981 tctcatgaaa atattgttgt gaacgaaagc gttcagtata ttaaggacaa cggtaatata
     5041 agagcatcga aatttgttaa tgcggtactt aggaatatat tgcgcagcaa ggataatctt
     5101 atcaaaaaaa tgaatgagct tccccgggat gattatttaa gtgttaaata ttcatatcca
     5161 aaggagttga tagttaaatg ggaaaaacag tttggcaagg atgttgataa ggtactaata
     5221 gccaacaata ccgaagcacc tttggaaata agagttaata ccttgaagat tcaaagagat
     5281 gagttgataa agctttttga agaaaagggt ataaaatctt acaaatgcaa atatgccgat
     5341 aagggattga ttattgaaaa cccatttgaa atagataaga cagatgaata taaaaatgga
     5401 cttttttctg ttcaaagcga aagttcaatg ctggccggac aaattttaaa ccctaaagaa
     5461 aacagcttta taatagatgt ttgcgctgct cccggcggca agacattaaa tgctgcagaa
     5521 atgatgaata atacgggtaa aatattatca agggatatat acccggcaaa gctaagacta
     5581 atagaaaatg aattaaaaag attggttata aaaaatgtta aagttgaaaa atatgatgca
     5641 agaaaactag atgaatcctt agtcggaaaa gcagattatg ttatagctga tgttccgtgt
     5701 tcgggactcg gtataataag acgaaaaccg gaaataaaat ataatgaaac agacttaaaa
     5761 gatataacag aaatccaata taaaatactt gagaatgcat caaagtattt aaaacaatca
     5821 ggcgaattgg tttattctac ctgtaccaca aacaaagagg aaaatattga aataattaat
     5881 agatttttaa aaaacaagaa ctttatttta gctgattttt ctgatattat aaatgaagat
     5941 tttaaaacag ccaaagacgg ctatattgaa atttatccgc atctccacgg tatggatggg
     6001 ttttttattg cgaaattgaa aaggttatga tataattgac aacaaaattc aattcataat
     6061 gtctattaaa gaagggtaat atgaaaaaaa ttgatgattt aagctttgag gaattaaaag
     6121 atgcagtgct taaaataggc gagcctaaat ttaaagcaaa tcaaatattc gactggatac
     6181 ataataagac tgtcgaaagc ttcgatgaca tgaccaattt atctcaaaaa accaaggcag
     6241 cgcttaaaaa agaatatgaa tttacaaaag caataattga attaaagctt gaatcaaaat
     6301 tggatgaaac aaaaaaatat gtaataaagt tggaagataa caacatagta gaaagtgttt
     6361 atttaaaata taaattcggc aataccgctt gcatttcatc acaagtggga tgcaagatgg
     6421 gttgtcattt ttgtgcttcc actaaaaagg gttttatcag aaatctgact gctgcagaaa
     6481 tgcttaagca aatatacttg attcaaaatg atataaatga aaaaatatct aatgtagtta
     6541 ttatgggctc gggagagcct ctcgaaaact tcgataatat agtgagattt ctaaatatta
     6601 taaatgatga aagaggccaa aatatttcca tgcgaaaaat tactctttcc acaagcggta
     6661 tagtaccaaa tatttataag ttggctgatg aaaatattcc gataacattg gcaatttctc
     6721 ttcatgcttc cacacaggaa aaaagagagc aaattatgcc cattgcaaga aaatataagc
     6781 ttcccgaaat attaaaagca tgtgattact atgttaaaac aacagggcga agacttacct
     6841 ttgaatatat aatgataaag ggttttaatg acagccgtga agatgcggaa aacttaagca
     6901 gattattgaa aaataaatta gcaaatgtta atttaattcc atgcaattat gtaaaagaag
     6961 caaatattga accaagcgaa gatttggata ttatgaaatt taaaaaaata ttgacggata
     7021 acggaattaa tgcaacggta agaagagaat tgggcagtga cattaacgct gcctgtggtc
     7081 agctcagaaa tgatttgtta aaaatgtgag gtactgtatg gatgtgttct ttgatacaaa
     7141 taagggttta atgcgagaaa ataatgaaga tagtttaata gtcgagcaat ttgataatta
     7201 ttgtctgtat gctgtggctg acggaatggg agggcacaaa gccggtgaag tggcaagctc
     7261 cattgctatt gaagagataa gaaacttttt cgttgaatgt atgaaggatg aaaattttca
     7321 gcccccatca ttcattatgg aaagcgtaaa gctgagtaat gacaaaataa gagaagtgtc
     7381 attaaataat gaagattgtt gcggtatggg aacaaccgta accatggctg taatagataa
     7441 gcttaataat ataatttaca ttggaaacat tggcgacagc agagcttatt tgttaaggaa
     7501 caatgaaatt aaacaaatta caactgacca tacttatgtg aatgagttga taaaggatgg
     7561 cagaattacg gaagaagaag ccaaaacaca tcctaaaagg aatgtaataa ctcgggcttt
     7621 aggcagtgaa gaatatgtac atgctgatat ttttgaattg gaattattaa aaaatgacat
     7681 actgcttctt tgcaccgacg gtcttacaac tcatgtatcc gatgataata tgttggaaat
     7741 cataaaagaa tacggcacat cagaaagtgt gcaaaaattg attaaacaag caaacgataa
     7801 tggcggaact gataatatta ccataattat tgttgataat ataattagag gtgaaactta
     7861 tgataggtag aacactagga aacagatatg aaattataga aaaaatcggc ggcggtggaa
     7921 tgtcaaatgt ttataaagct aagtgcaatg ttttaaaccg ctttgtagct ataaaaattt
     7981 taagggatga attaactcag gattctgatt ttgttaataa cttcaaacaa gaatctttat
     8041 cggcggcaag tctcgctcat cctaatattg taaatattta cgatacagga tttgaagacg
     8101 atatctatta tatagttatg gaatatgtaa aaggggaaac tttaaaaaac tatataaaaa
     8161 gaaaaggcag tctgagtgaa aaggaagccg tgaaaatttc aagacaggtg gcagaagctt
     8221 taaagcatgc tcactcacac aatatagtac acagggacat taagcctcac aacatacttc
     8281 ttactgagga cggaacagca aaggttactg atttcggaat tgcaagagca tcaacaagtt
     8341 ctaccataaa caacacttca aatgttatcg gctcagtgca ttacttttct cctgaacagg
     8401 caagaggagg atatgttgat gaaaaatcag atatttactc attgggaatt gtaatgtatg
     8461 aaatggtgac aggtactctt ccttttgatg cagataacca tatttcggtt gctatgaaac
     8521 aaattcagga aaaacccgtt ccgccttcaa aaagggtgaa aaatgttaaa atatcaaaaa
     8581 attatgaaga tattataatg aagtgtcttg aaaaacatca aagcttcaga ttccaaaaca
     8641 ctgatgaact gctgaaaaag cttgatgcat tgaacggaaa taataatccc gtaaaagacg
     8701 aaacggaaat aatagattca ccgactattg aaattcctgc gcttaatgat ttagaagaaa
     8761 atgttattaa tatagattta attgacgaaa attccgacaa agcatttaaa agcttcttta
     8821 gcggaaatga agaagatgaa aatgttgaaa agagcataaa caataaaaaa attactatag
     8881 cggcaatttt aagcgcattg gttatagcag taattggcgg agtaatttta tttaagtcat
     8941 ttttgtatgt gccggaagtt ccggtgccga gtttattggg aagaagtgaa gaggaagcca
     9001 agaagctcat tgaagatttg ggtctggtat tcaaagtttc aaaaagagaa tattcttctg
     9061 aatacgatga aggttatgta atggagcaaa gtattgatga gggctctaag gttaaagaaa
     9121 attaccccat agaagtggta ataagcaggg gagaaaaaga aatagttgtt cctaatttaa
     9181 taggcaagta tgctatagag gcgggaggaa ttctcaataa tgcagggctt tcagaaggag
     9241 aaacgacaga agaaaattct gacacatatt tggcaggaca gataattgat caatatcctt
     9301 tacctaatac accggctagg gaaaatgata aagtaagcta tgtggtgagt ataggtccga
     9361 agattatata tgtgaaaatg ccaaatgtgt taaatttgaa tttagaaacg gcgaagcttt
     9421 cgattgttca acatggtctt gcggttgggc aggtaaatga agagcccagc gaagaaatag
     9481 aaaagggctt ggttatgaga caaagtataa cagccgatca ggaagtcgaa caaggaacat
     9541 caatatggct tacggtaagt tcgggcaagc cggaagaacc tgacgagcct acaccggaag
     9601 aaggagtatt tcccttggca atatctcttc ctgcagacag ggatagtgta tcaatagtga
     9661 ttcaaagaat agccagtgac ggacgtgaag ttgtgtactc agaggaagtg gatacttcgg
     9721 aaggaaatat cctagttaat gttaagggaa aaggaacagg aatattcgaa atatatattg
     9781 acaatgaatt atatgacaga gttgaaataa catttgaata aaaaggagtt ttaatgattg
     9841 agggaatcat aacaaagggt gtcggaggaa attattatgt tgatatcggc agcaaaataa
     9901 tagaatgccg agcaagaggc ttgttcagac tcaaaaatat caaacctttg gtaggggata
     9961 aagtccttat caggattaca gaggaagatg aggatatggg ttatatcgaa gaaattaaag
    10021 agagaacaag cgaaattaaa agacctcccg ttgccaatgc tcagcagctt gtaataattt
    10081 tctccgtaaa aaacccggaa ccaagcttta tattgttgga taaacttttg attgcagcaa
    10141 agtcaaataa tttaaaccct gttatttgta ttaataaatc agatttagca aatgaagaag
    10201 aaaaagaaaa tatacttaac atatttaaaa acacaggcta caaaattatt tttacaagca
    10261 agtatgatga ggattcccta cagaatttaa aagatatcct taaggacaga ttaaatgtgt
    10321 tttccggacc ttcaggagtt ggaaaatcat ccttaatgaa tgccatagag cccggatttt
    10381 atttggaaac aggtgaaatc agcgataaat taaaaagagg aaggcatacg accaggcatg
    10441 cggaaatatt taaattaggc tttggcgggt atgcggttga tacgccgggt ttcagttcct
    10501 tcgaattgga aggaattgat gagtattctt taaaaagtta ttatccagaa atagtaaaat
    10561 atgatgatgg ctgtaaattt ttagactgcc tccattacaa agagcccggt tgtgtgataa
    10621 aagaagccgt taatagtgat ttaatcagca gagtaaggta taataactat ataaagcttt
    10681 tggagcaaat aaaagaaagt aagccttatt aaggaggaag tatgaaaaat aaattagccc
    10741 cttcaatttt gtctgccgac ttttctaatc taggagaagc cgtaaaactt gtggaagaag
    10801 ggggagcaga ttacatacat atagatgtaa tggatggtca ttacgttccc aacattacaa
    10861 tagggccggc agttgtaaaa gatttaagaa aaacaacatc tctcatattt gatgtgcatc
    10921 ttatgattga gaatccggat aattttatcg agcactttta taaagcagga gcagatataa
    10981 ttaccgtgca tcaggaagca accgtacatc ttcacagaac tatccagaaa ataaaatcat
    11041 acggcttaaa agcaggagta tcattaaatc cggcaacacc catatctgtt ttaaatgaaa
    11101 ttattaaaga tgtggatatg gttttgctga tgagcgtaaa cccgggtttt ggaggacaaa
    11161 gtctgataga aaatgtcaag tataaatttt atgatttaaa gcaattatta aataaatata
    11221 atttagatat tgatattgaa attgacggag gcgtgaatta ttcaaattta aaggaaattt
    11281 taagctgggg tgcaaatgtt atagttgcag gctctgcaat ttaccatgca gaggatattg
    11341 ttttggaaac aaagaaattt aaggaaatca tgtcatgaaa acagcgctga ttgtcggaaa
    11401 cggagatgaa accgccaaaa gaataattga aaatattaag tatgattatt tgatatgtgc
    11461 ggatggaggg cttgaaaaag tttcaaatta cggaataatt ccagatataa taatcggtga
    11521 ttttgattcg gtagctagta atgttctgaa acaatatgaa aacagaatac ctattgaaaa
    11581 atttccttcc gaaaaggatt ttacagacat ggaattagct gttgaactgg ctgtttccaa
    11641 aggatacaaa aatataatac tcacgggagc ttccgggtca agattagacc ataccttggg
    11701 caacattttg cttatggaga aatacttcaa agatggagtt aatatttcaa taattgataa
    11761 caataatgaa atgaaaatta tttccgataa ttcggagctg tttatagaat acaaagaagg
    11821 ttattttata tcgataatcc ccataacaga tagtattcag ggactatgtt tggaaggatt
    11881 caaatataat cttgacaatg ttgatgttga aagaggctcg actctttgta tcagcaatca
    11941 aataaaagat aataagggaa aaattacgtt aaaaaaagga agagctatag taattatatc
    12001 taaagattaa caggagggtt catgaaaaca acggatataa aagatatatt cttatcgccg
    12061 gaaaattaca aagaagtccg tatagagggc tggataagaa caataagaga ttctaaaaat
    12121 tttggattca tagaattaaa tgacggaaca tttcttaaaa atgtgcaggt tgtttttgaa
    12181 aacaacttga gtaattttga agaaattaaa aaattttcaa caggcagtgc cattacggta
    12241 agaggaaaca tagttttaac tcccgaagca aagcagcctt ttgaaattaa agcaacagaa
    12301 atagccttgg aagcaccgtc agattcaagc tatcctttgc aaaagaaaag acattcaatg
    12361 gaatatttaa ggactattgc gcatttaaga ccccgcacca acactttttc cgcagttttt
    12421 cgtgtaagga gcttagctgc ttatgccata cataaatttt ttaccgaaag gggatttatt
    12481 tatgcgcatc cgccgataat tactgcttca gatgcagaag gtgcaggtga aatgttcaga
    12541 gttaccacac tggatttgga taatattgaa aaggttgacg gaaaaacgga ctattcaaag
    12601 gactttttcg gaaggtctgc aaatcttacg gtaagcgggc aattagaagc tgaaattttt
    12661 gctttggcat ttaagaaaac ttatactttt ggacctacat tcagagccga aaattcaaat
    12721 actgcaaggc atgccgcaga attttggatg atagagcctg aaattgcttt tgccgacata
    12781 aatgataata tggatttggc agaagatatg gttaagtatg tcgtaaatta tgtcatggaa
    12841 aatgccaaag aagaactgga gtttttcaat aactttgtgg aaaaagggct tttagaaaaa
    12901 cttgataatg tggtaaattc agagtttgga agaatatcat acacagaagc aatagatata
    12961 ttgatgaaaa acaatgataa tttcgaatat aaggttaaat ggggaacaga tatacagact
    13021 gagcatgaaa gatatttgtc ggaaaaaata tttaaaaagc ctgtatttgc agttaattat
    13081 cctaaggaaa taaaggcatt ttatatgcgg atgaatgaag ataataaaac agtggcagca
    13141 atggacttat tggttcccgg aataggtgag ttgataggcg gcagccaaag agaagaaaga
    13201 cttgaatttc ttgaaaagcg aatggatgaa atgggagttc caaaagataa tatgtggtgg
    13261 tacttggaat taaggaagta cggcggtgtg aaacactcag gtttcggact tggttttgaa
    13321 agacttatta tgtatttaac aggcatgagc aacataaggg atgtaattcc gttcccaaga
    13381 acccctaaaa atgcagattt ttaaggtata ggtaagatac aaagaaaatt tctttgtatc
    13441 ttttttttat gcttgaatag aatgtaataa tagtagagct ctacataaat tctaaggggg
    13501 atattatgaa ttactttaag cataaaaaga aatgcagtcc tgcaaataaa ccatcatttt
    13561 ttaaattcac cgaaatatta ggccttatga ttgcaaccat aggagcaatt attatagttc
    13621 aaatgcttcc tgttaaagta tggttgtttg tgttaggaat attattgata attttgggtt
    13681 gcacattatt caggctgtta taggtatggg gacacacctc taatagatgg acagtctccg
    13741 ggggaatgag gaatgtcccc aatttggtgg ggacacaaaa aaataatggc gttggccact
    13801 attttaatga tagatgtgct taatttgcac gttgtatttt tcctgctcta atacatcttg
    13861 tacaaactgt ttttcttaaa gctcttccgt cttccataac cgtgatagtt cttacgttcg
    13921 gtttccatgt tctgtttatt tttctatcgg aatgcgttat gctgtgtcct gatatagttc
    13981 cttttccgca tacttcacaa aactttgcca taattccacc tcccatttat attaacgtat
    14041 acatcattat ttattatata agttactgtc acacacagtg tcatccttag cgaagcaata
    14101 tttcgtaaga gcgtaactta tttgaacttc atcattctaa cattaaaaat taaatattgc
    14161 aatattaatt ttaaattagt ataatgaata gtgctacttt agaagcagaa atttattcag
    14221 gaatcaagta aattatatta taattgtaaa ttcatgttaa taaggttata atgataaata
    14281 aagtattaaa taggaggtta gtatgtcagt taagataatt aacgaaaatg gaagtatata
    14341 tgttgatgac caggccttag ctgcaattgc aggattagct gcaatggagt gttatggtgt
    14401 tgtgggcatg gcttccagaa atgcaactca aggttttttt gagctgctga aaagagagca
    14461 gttaaccaaa gggataaaag ttacatcaac cgaagaagat aaaataaata tagatttata
    14521 tatagtattg caatatggag taaaaatatc cgttgtagca gaaaatgtaa tttccagaat
    14581 taagtacagc gttgaaactt tttcgggagt aagcgttgaa aatgttaata tttttgtgca
    14641 gggtgtaagg gtgcaaaagt aatcaggagg ctaaaatgtt aattaagtac atagataatg
    14701 tcaaattcaa aaagatgctt ttgggggctg ccgttaacct tgaaaacaat aaaacaattg
    14761 tggattctct aaatgtattt cctgttcccg acggagatac cggtacgaat atgaatctta
    14821 ctttgcaggc agctataaag gaaataaaca atctttcaga aagcacaatt acatctcttg
    14881 caagtgcggc agctaacggc tcacttatgg gagcaagagg aaactccgga gtaatattgt
    14941 ctcaattgtt aagaggaatg tcaaaagcac ttaagggtct taatcatata gattgcaaaa
    15001 taatagcaga atcccttaaa tcagcctcag atactgctta taaagcggta atgaagcccg
    15061 ttgagggaac aatgcttaca gttgcaaggg aaacggcaga aaaagcaatg gcaacttata
    15121 cagattatga agacgtaata gaatttatag aaagtcttat tcagcaagca aaagatactt
    15181 taaatcgcac tcctgaaatg cttaatgttt taaaacaggc agatgttgtg gatgcaggcg
    15241 gaaaaggatt aatatatctt atggaaggtg cacttgcggc actaaaagga gtagaaatca
    15301 aacaggagat aacagagcag gccgtgattg atacagaagt tgaattagat gaatttacga
    15361 gcgaagatga aattatattt gcatattgca ctgaattttt gattaaaaat cccataaaga
    15421 cggcagagga atttttaaca agaatttatg acaagggtga ttccatagtt tgcgccggca
    15481 atgaaaatgt aataaaaatt cacatacaca ccaacaaccc aggccaaata ttggctatgg
    15541 cagttaaatt cggtgagctt tcaaacatta aaattgacaa catgaaggag caatttaggg
    15601 aaagacttaa ggaaaatcac aaaaataatc agaaaaaaaa gaaatacggt tttgtttcca
    15661 taggcgtagg aaagggaata gaaaaagtat ttactgattt aaatgtagat gtgtttttgt
    15721 ccggcggaca aacaatgaat ccaagcacgg aagatatatt ggaagcagca aacgacataa
    15781 atgcagagca cattataata ttgccaaata attccaatat aatattagct gcaacacaag
    15841 caaagaaaat ttcggacaaa aatttatatg tggtgccaag caagactata ccccaaggta
    15901 tagcagctct tattgcattt aaagaagaca gtgatataga cgagaatatt gaagccatgt
    15961 cacaggcggt aaaggaagta aaaacagggc ttgtaactta tgctgtaagg gatactgtat
    16021 ttaatgattt agaaattaaa aaagaccaaa ttattgctat ttatgaaggc gatataacaa
    16081 atcacggaga aaatccggaa gacgaagcag ttgaattaat aaataaaatg gttgacgaag
    16141 acagtttttt agttaccgta ttttacggag ataatgtcga tgaaaaaact gcagaaagta
    16201 ttaaaccaag gattgaagag attgcagaag actgcgatat tgaaattata tacggaggtc
    16261 agcctcttta ttactatata atctcggtag aataacaaaa caaggcaggc agacttctat
    16321 aacatgtagt ggtgaaaaat gttagatgca gatattcaat atttaaaggg agtagggccg
    16381 aacagggctt taaaattgca caaattagac atatttacag tgagcgattt attaaattat
    16441 tttcccgtta aatatgaaga caggagaaca gttaaaaata taaatgaaat atgtgagcag
    16501 ggtaaatatc tgttaaaagt caggattttt gaaaaaccca aatattcaag aatcaaaaag
    16561 aatatgtcaa taattaaagc aatatgtaag gatgatacgg gatttgtcac ccttacattt
    16621 ttcaatcagg actttttaaa ggataagctg attgttggcg aatactacta tgtttttgga
    16681 aatgtaaaaa ttgcctatgg caaattagaa tctacaaatc ctgaaatcga atttattaat
    16741 aaagactgga aggcaggaaa aataatgcct gtttacggtc ttacatacgg attgtcaaat
    16801 aatgaaatta caaagttagt aaagactgcc ttagaaacat actatgatga aattcaaaat
    16861 gtcttgcctg acgaaataat aaagaaatat aagcttattt caaaaaaagc tgcagtaaaa
    16921 gctcttcatt ttccaagcga caataagaaa tatacattgg caaagagaac tattgccttt
    16981 gaaaaattgt tgataatgca gcttggcctt tttaatatta agaataattt aaccaatgaa
    17041 ttaaatgata tcgaattaag aaacacagag ctgtcagaaa aactattgag ctccatacct
    17101 tacaagctta ctaaggcaca gcttaaagtt attgaggaaa taaaagaaga tttgaaaagt
    17161 caaaaaacaa tgaacagact tgttcaggga gatgtcggct ccggaaagac cgtggtagct
    17221 gtttatgcaa tgctaatggc agccggctcg ggatatcagt catccatgat ggctccaacc
    17281 gaaatattag ccctgcagca ctatgataca attaaagagt atataaaaat agccggattg
    17341 gatgttaagg cagaaatttt aacaggttca acaaagccaa aagaaaaaca agaaattctt
    17401 gataaactca aaggcggaga aatcgatatt atagttggaa cccatgcgct tattgaagaa
    17461 aatgtggagt ttaaaaatat agggcttgtg gttaccgacg aacagcatcg ctttggggta
    17521 aggcaaagag caaggctgtc aaataagggg aaaaacccgc atattcttat tatgacagcg
    17581 acacctattc caagaacctt ggctttgatg atttatggtg atttggatat ttcattgata
    17641 gacgaaatgc ctccgggaag acaaaaagta attacttcta tcataagcga aaaggaaaag
    17701 acaaaagcat atgattttat taaaagtcaa gttgacagcg gcaggcaggc atatattgtg
    17761 gcacccttgg ttgatgaatc cgaaaattta gatttgaatt ccgctgttga aatatataaa
    17821 gaattaagca cctcacattt ttcagggtac aaattaggtc ttttgcacgg taaaatgagc
    17881 aatagtgaaa aagaagaaat cataaataaa ttttataggg gaaaaataaa tgtgcttatc
    17941 tctaccaccg ttgtcgaagt tggcgtaaat gttccaaatg cagttgttat gcttatttta
    18001 aatgctgaaa ggtttggtct tgcacagctg catcagctaa gaggcagagt cggaagaggt
    18061 aaatgccagt catattgtat tttggttaat gaaagtaaga gtaaaaaatc aaaagataga
    18121 atgaatattt taaaaagaac agacaacggg tttataatag cagaagaaga tttaaaaata
    18181 agaggacccg gagacttttt cggagtaaga cagcatggat tatcaaaatt ggaaattcaa
    18241 aatatagtga ataatgtaga aacagtacag gaagtccaac ttttatcaaa aagaatttta
    18301 tcggaaaacc catatttaaa aggttctgat tatcaaaatt taaaaatgga aattatgaat
    18361 ttgtttaaag acgaaacaat tgtatttaat tgataaataa aataaaaaga ggttctaatg
    18421 agaataatat ccggaacaaa taaaggcaaa aaattgtatg ctcctgaagg gaaaagtgta
    18481 agacctaccg gtgataaaat caaagaggcg atttttaata taatcgggtc aatcgatgaa
    18541 gagtcgactg tattagaatt gtttgcaggc tcaggaagca taggcatcga atttttggca
    18601 agaggtgcaa aacactgtac atttgttgat atatctcgta aaagtttaaa ttatgtaaaa
    18661 aagaatttgg aattatgtaa ttttagcgat aaagctaaaa ttatatcctc tgattatgaa
    18721 aaagcaataa taaatttatc taaggacaat gaaaaatttg attatatttt tgcagacccg
    18781 ccatacgagt taaacataag caataaaata ttaaataaaa ttgttgaata tggtatatta
    18841 aaaagcgacg gactccttat tattgaaagc gacaaatccg aaaaagtaat tgataatgaa
    18901 gttgcaaata tgatagaata taaagagaaa atatatggaa ggactaaaat cagcataatc
    18961 aaatatttgg aggaacaatg aaagtattat acaccgggag tttcgacccg ataacaatgg
    19021 gacaccttga tataattaaa agaatttcga aaaaatttga agaggttgta gtaacggtat
    19081 ttaataataa ggagaagaaa tactggtttt ccatagaaga aagatgtgag atggtaagag
    19141 aagccgttaa ggatttagaa aatgttgagg tggattttac gggagggcta gtagtagatt
    19201 attgtaaaag gaataatatc agcattatag taagggggtt gagagccgta tccgattatg
    19261 aatatgagct ggcaatatca agtattaata aacatttaaa taatgaatta gaaactatat
    19321 ttttggttgc aacatcggaa tattcttttt taagttccag catggtaaaa gatgtggctg
    19381 aacatggagg aaacattgaa ggattagttc ctgataatgt tgcacaaaat atatatagat
    19441 tacttgggag gaagtaaaaa tggatatctt gaatttattg gaaaggatag aggatattat
    19501 agaagactct tctaagtttc ccttatcaaa taaggttatg attgacaaag aagaagtttt
    19561 ggaagtaata aatgaaatca gattaaaatt acctgatgaa atcaacagag cttcatgggt
    19621 tgcaaaagag agacagcgta tacttaatga agcacagagc gaggcagatg aacttatcaa
    19681 taaagtaaaa gagcaacaga aacatttaat tgaagaaagt gaaataacaa gacaagctaa
    19741 aatttatgca aatgaaataa ttgaggaggc agaaagaaaa gcaaacgaaa tgaaagttgg
    19801 agcatataat tacagcgatg aaatattgtc caagcttcaa gacaaaatca aagaaataaa
    19861 caacataata gaagacaata gagaagtact taaaaagatg taataataaa taaagaacca
    19921 tcccgataat taaattagga ggttctttaa attttattta tttgtgctca attcttctgt
    19981 gattttatgc ctgtatacct ccatcatttc ggagaaaatt ttaccggttg gaggcggagt
    20041 gtaagtaata atgtttgcac ctgctcttat ggtttccaat atactttcag gtgtcggccc
    20101 acccgttgca ataatcggaa aatatgggtg tttttctcgt atttgtctta caatatttgt
    20161 tgtatttgct cctcctgaaa cgttgaaaat ggatactcct gcctttattc ttgattcgaa
    20221 atcctctttt tcggacacta ttgtaattat aatcggtata tccaatctct ctttcatttc
    20281 ggatatcagt tcattcttag taggcgcatt caatactacc ccataggctc ccgtcaattc
    20341 ggcatttaaa gcaatatcaa ttgatctctg tcctgttgtt gtacctcctc cgacacctac
    20401 aaacacaggc gtaggagata tatctataat tgcctggtta attgccaatt gaggagtaaa
    20461 aggatatact gcaattaccc cgtctgcatt attattcttt ataattgcta aatcagttgt
    20521 atagattaag gactttattc ttttcccaaa aatttttata ccgctggcat tgtatatttc
    20581 ttgcggcatc tgtatgggtt tcttttttag aacggattca atggtcggta caaacttttt
    20641 atccttaatc tcagtgtttg attttacggc ttcactgtaa ttatcggtaa taatttcttt
    20701 attatccata tacgactcct ttttagatta ttatactttt tttattaata aaattcaaca
    20761 caaataaaaa taaagctatg caattttata aataaaaggt ataatacaag tagtacaatc
    20821 agcggaggtc attttgaaaa aattaatgct ttcagggact gttcagattg aaacaagaag
    20881 atttgtactt aggaaattcc aaatgtccga tgcacaagat atttattata attggttaag
    20941 cgacaaggat tcagctaaat ataatgcatg gagggttcat acgtctattg atgaaacaaa
    21001 agaatatcta tcggaatgga cgagccttta taaaaaagat gattatttgc actgggctgt
    21061 tgtggacaag gaaacaaaag aagtaatcgg atcagttacc gtatcaaaca ttaaaaagtg
    21121 gaaaaaatat tgtgaagtag gttacactat agcaaaaaaa ctatggaatc aaggaattgc
    21181 tacggaagta ttaatcagta tactgcatta tttaacacaa gaagttggat ttgaaaaagt
    21241 ttgtgcaatt catgatatta gaaatgaagc atcagggaga gttatggaaa aggcaggaat
    21301 gaaatttgta aaaaataaaa ctcacatttt tttaaacagc gataatataa taatgaaatg
    21361 cagattatat gagtacagag gtatggggac acacctctag tataaggaaa gtctttgcaa
    21421 gttcgaggaa tgtccccaaa aataaaggtt gctttaaaga tgttttatgt tataatataa
    21481 tggttaattc atgaatctaa ggtcaataat tgaatgatac atagaaactt aaggataacg
    21541 gagggctaaa tgagttcaaa attaataaaa aaagagaaaa atacagttac attagaattt
    21601 acagtatctc aagatgagtt tgataaggct gtgaataaag catatttaaa ggttaagaat
    21661 aatataaatg ttcagggatt cagaaaggga aaagctccaa gacatattat agaaaaaaaa
    21721 tatggaaaga gcatttttta tgatgatgct ttggatattg ctgttcagga agcatatccg
    21781 aaagctgtta atgaattaga attagacatt atagacagtc ctaaaataaa tgttgaaaaa
    21841 tttgaagatg gtgaagaaat agtatttacg gccgaagttg aagtaatgcc ggaagttgaa
    21901 ctgccgaagt acaaaggaat tgaggttgta aagacagaaa taaaagtaac tgacgaagat
    21961 gtagaaagag aattaaaatc aatacaggaa aaaaatgcga gaatagttga agttgcggac
    22021 agaccagttc aaaaaggcga ctttttaaca attgattatg ccggttttgt cggagaagaa
    22081 caatttgaag gcggtacagc cgaaaatcaa acattggaaa tcggctcgaa ttcttttata
    22141 ccggggtttg aagaccaatt gataggcaag tcaaaaggtg atgaggtaac agttaatgta
    22201 acattccctg atgattatca tgcagagaat ttaaaaggca aggatgcagt tttcaatgtt
    22261 aaaatacatg aaatcaaagc aaaggaactt cctgaaatgg atgatgaatt tgctaaggat
    22321 gtaagtgaat ttgatacatt ggaggagctg atagaggata ccagaaaagt attggaaaaa
    22381 aaggctgccg accaatctaa aatctcaaac gacaacaaag ttataacaaa aattgtaaat
    22441 gatacaaagt tagaagttcc cgaagtttta atccagaggg aaattgagca tatgggaaga
    22501 gattacgagc agcagttccg tcagcaagga tttaccggca aagaatacga cgagttgatt
    22561 aataattttg ttaatcaata taaggaaagt gccagaggtc aggctgagtt caatgtaaag
    22621 gcagaattgg ttttagaaga aattattaaa aaagaagaaa taacagtttc agatgaagaa
    22681 ttacaagagg aaataaataa aatagcagag tcatacaagg ttgaagaaga cagacttgaa
    22741 ggatttaaag aaagtttaat gcagtcaagc aaaggctata ttgaagaaac tcttaaaaaa
    22801 agaaaagtct ttgatatgct tgttgaaaat gcggtttttg tcgatgctcc tgagactgtt
    22861 gaatcaatag aagctcatga aacaattaat gaaagcgaaa aagcagaaga gccggaagaa
    22921 gacaaagaat aagattaaga taaatttaag aaaattaagt acaattaagg tagaaataaa
    22981 tataatatag gaggaataaa gatgacatta ataccatatg taattgaaca gaccggtaga
    23041 ggtgaaaggt cttacgatat attttcaaga cttttaaaag atagaattgt aatacttagt
    23101 gatgaagtta atgatgcgac ggcaagctta atagttgcac aaatgttgtt tttggagtct
    23161 gaagacccgg ataaagatat acaattttat atcaattcac ccggaggctc cattactgca
    23221 ggcatggcaa tttatgacac catgcagtta ataaaacctg atgtttcaac aatatgcgtc
    23281 gggatggcag ccagtatggg agcattcctg ctcttgtccg gagcaaaagg aaagagattt
    23341 gctcttccaa attcagaaat aatgattcac cagcctttgg gaggcatgag agggcaagct
    23401 tcagacataa aaatacatgc cgatagaatt atcaagataa gagatacatt aaacaagatt
    23461 attagcgaaa aaacaggtca accgatagca aaggtacaac aggatacaga cagagataac
    23521 tttatgactg cggaggaagc taagatctat ggcataatag atgatattat agctaaaaga
    23581 aaataaggag tgtttcaatg agcaaagtaa atgaaaaaca tattaaatgc tcgttttgcg
    23641 gcaagtctca ggagcaggta agaagactta tagccggtcc ggatgtttat atatgtgatg
    23701 aatgtataga gttatgctat aacattgttg atgaagatat agatttattt gaagaatatg
    23761 attttacgga actgccaaaa ccaaaagaaa tgaaggcaaa attggatgag tacgtaataa
    23821 atcaggataa cgccaaaaaa gctttggttg tagcggtata caatcattat aaaagaatta
    23881 actacaaaac taaagatgac ggagtagaaa ttcaaaaaag caacatacta ttgttgggac
    23941 ctacaggcag cggaaaaaca tatttggcac aaacattggc aaaattatta aatgtaccct
    24001 ttgccatagc tgatgccaca tctttaaccg aagcaggata tgtaggcgaa gatgttgaaa
    24061 atatcctttt aaaattattg caggcagcgg actttgatgt agaaagagcc gaaaagggta
    24121 taatatatat cgatgaagtt gataaaattt caagacgttc agagaaccct tcaataacaa
    24181 gggatgtaag cggcgaaggt gtacagcagg cattgcttaa aatacttgaa ggaactgttg
    24241 ccaatgttcc gccccaggga ggaagaaaac atcctcatca ggaattcatt caaatagata
    24301 ccaccaatat attgtttata cttggaggag catttgatgg tatcgagaaa ataattcaaa
    24361 gaagagtggg taagaaaagc attggttttg gagcagatat ttcaggaaac aactcaatgg
    24421 atacggatga attgttaaaa caggttcaat cccaggattt attgaagtat ggaatgattc
    24481 ctgaatttgt cggaagaata ccggtaattg tagctttgga aaaattagat aaaaatgctt
    24541 taatatcaat tctgacggaa ccaaagaact ccttagtcaa acaatacaaa gaattattta
    24601 aaatggataa cgtagagttg gaaatcgaga aagatgctct ggaagaaata gctaagaagg
    24661 cattggaaat caatacggga gcaagaggct taagaggaat tcttgaaagc acaatgactg
    24721 aagtaatgta tgaaattcca tcaagaagtg atatcaagaa atgtgttatt actaaagaca
    24781 caattataaa taaaagtgag ccgaaattag tattaacaaa gaagcctgtt tctgataaga
    24841 aagagaatgt ttcatagact gcctgccatt agcaggcagt tttttaagga gaaagtatga
    24901 ttaagaatta taaaattgag agaagaaacc ttccgctgat tccgataaga ggcataggaa
    24961 ttttccccaa taccgtaata cattttgaca taggaaggga aaaatcgata aatgcattgg
    25021 aagaagcaat gcttgaagat tcagatattt ttttgaccgt acaaaaggtt gcggacatgg
    25081 agtcgcctaa ggaagatgat ttttatgaag tgggcgttat ttgtaaaata aagcaaatga
    25141 ttaaaatgcc cggagacaat atcagagtcc ttgttgaagg tataaacagg gctaaaattg
    25201 tatctgtaac ccaggatgac ccctattttg aagttgcttt ggatgagtat atatatgacg
    25261 acagtcttcc tattgatgat aatttggagg caatggttcg tcttacatta aacaattttg
    25321 aagaatatgc tttaatttat gcaaaaattg caccggatgc cgttatatca cttaaagaaa
    25381 taaaaaatcc cgataagctt gcagatgttg ttgcttcata tatttatctg aaaacaaatc
    25441 aaaaacaagc tcttctggaa atttttgacc cttataagag gcttgaatcc ataaataaaa
    25501 tgttagcgaa agaaattaag gtgctgcaga tagaaaatga aataaacgag cttgttaaaa
    25561 aacaggtcag cgagtttcaa aaagattatt atttaaaaga gcagatgaga gctattcaaa
    25621 aagaattagg ggaagatgaa ggtacggatt atgaagttga agagtatctt caaaaaatct
    25681 caaaggccaa catgcctaaa gatgtaaaag aaaaagcaga aaaggaaatt aacagacttt
    25741 taaaaatatc tccatcttca ccggatgccg gggttataag aacgtatgtt gattggcttt
    25801 tagatttacc ttggaataaa aagacaaagg acaataccga tttaagcaga gccagaaaaa
    25861 tacttgctga agaccattac ggattggaag aagtaaagga aagaatattg gaatatttag
    25921 ctgtaaagaa tattaaaaag gatatgaagg gaccaatact gtgctttgtg ggaccaccgg
    25981 gagtagggaa aacatcaatt gcaaaatcca ttgctcgcgc catggataga aagtatgtgc
    26041 gaatttcttt gggaggagta agggacgaag cagaaatcag gggtcacaga agaacatata
    26101 tcggtgcaat accgggaaga ataatatcat caattgcaaa agtaaaagtg aataatcccg
    26161 tatttttatt ggatgaaata gataagttaa acagtgattt taaaggtgac ccggcttctg
    26221 cactgctcga agcacttgac cctgagcaaa acaatacatt taccgaccat tatattgagg
    26281 cacctttcag tttgcaaaat gtaatgttta ttacaaccgc aaacacgacc tcaaccattc
    26341 ccggtcctct tttggacagg atggaagtta ttgaaatatc aggatacaca gatattgaaa
    26401 aaagaaatat tgcagaaaaa tatttaatta aaaaacaact ttccgagcat ggacttaaag
    26461 aaaaccaggt aaaaatttca acaaatgcaa taaatgaaat aatcgaaaga tatacaagag
    26521 aatcaggagt cagaaactta gaaagaaata taggtaaatt aataagaaaa gctgcggttc
    26581 aaatagtaga aaaaaacaaa agcactgttt caataaataa tttaaacata aaaaaatatt
    26641 taggcatccc taaatatgat tatgatattg cctatgagga agaccaggtt ggagttgtaa
    26701 caggacttgc ttggactcag gtaggcggag aaacactgtt tgttgaagtg aataccatga
    26761 agggtgacgg gaaactacag cttacaggac aattaggcga tgttatgaag gaatctgcaa
    26821 tggctgcatt aagctatata aaagccaatg cggaaaattt cgatattgac aataaaatat
    26881 ttaaagaaac ggacatccat gtacatgtgc ctgaaggtgc aattccaaag gatggtcctt
    26941 ccgcaggaat taccatggca acagcaataa tatcggcttt gacaaagaaa cctgtcagaa
    27001 aagatgttgc gatgaccggt gaaattacaa ttacgggaag agttcttccc atcggaggcc
    27061 ttaaagaaaa acttttggct gcaaagagag caggaattaa aaaagtttta ctctgctcta
    27121 aaaacaaggc ggatgtttcg aaaattgacc caaaaataat tcgctcaatg gaaattgtat
    27181 atgcagataa aatagatgat gtggtgaaag aagcgttgcg ataagatata aaaaatccgc
    27241 taacggtttt ggccaagaat aagaagggat atatatgatt atcagaaaag ctgaattttt
    27301 gatttcagcg gttaaaaaag agcagtatcc gaatactgga gttcctgaaa tagcatttgc
    27361 aggcaaatca aatgtcggca agtcttctat gataaatgct ttggtcaaca ggagaaatct
    27421 tgcgaaaacc agctcacagc ccggaaaaac acaaaccttg aatttttaca atataaatga
    27481 tgaattcaac tttgtggacc ttccgggata tggctatgca aaagtatcaa aggcagaaca
    27541 agctaaatgg gcagatatga ttgatttata cttgcacagc agacaacagc ttaaggaagt
    27601 tatactgctt gtagatataa ggcatgagcc gggcagaaac gacctgctga tgtacgaatg
    27661 gattaaaagc tttgggttca aaggatatat tatagctaca aaagcagata aattatctaa
    27721 gtcccaacag attaaaagtt tgaataatat taaaaaaacc ttacaaataa aggatatagg
    27781 tttaatatat caattttcat caacatccaa acttggagta gaagaaatat ggatactatt
    27841 tgaaagcatc ttaaatttac atgattagac aaacccctgt aaaaaataag aatacctttg
    27901 tgttataata tattttgctg tttaagctca ttaaaacagg gtatattatg acgaggagga
    27961 attattatgc attatgtagt tgaaagaaaa gttaatagcg acattaaatg cggcaagaag
    28021 ataatcgaag agattcttgg aagcattgag ggactgattg atcccaatac ctatttcaac
    28081 acaaaactta ttcttaatga actgatgata aatagtgtcg tccacggtaa ttgtggagac
    28141 ttaaataaaa tgatatatat aaaagtctat attaacgaat caagaattat tgttgaggtt
    28201 tcagacgaag gttcaggtat ttgctatgag cataaaaatt gcggggaata tgattatttg
    28261 gaatgcggaa gaggtttaat gctggtggaa ggactttccg acaagttaag cattgaagga
    28321 aatacggtta cttgtgtaca atatttaaaa taattattta aataacatac aaaatttgct
    28381 ataataaaca ctacgagtgt ttatttttta tggtatgggg acacaccgat gtccctgttc
    28441 aaggagtaat aatggacaat ataaaacttt ctgtcagaga attggtcgaa ttcatttata
    28501 aaagcggcga tattaatgta agaaatttag gtgcagatag agcaattgaa ggaattaaag
    28561 cccataaaat tctccagaat caaatgggag aaaattatta taaagaatac tatctgaaag
    28621 gcgaatttgt ccttaacgat attatatttt tgattgaagg cagagcagac ggaattttaa
    28681 ttgaagacga tgaaacaata attgatgaaa taaaatcaac ttatacggat ttaagtttga
    28741 ttaatggtga atacagcacc gcccatatgg ctcaggctaa atgctacggt tatatgtacg
    28801 gactctcaaa caatttggaa aatatcagtg tgcagcttag gtactataat cttgacacca
    28861 aagaagtgaa aacaataaag cagacatttt caacagaaga attaaaaata tttttttatt
    28921 ctcttcttaa tacatatatt gattgggcag aaacaattat aaatcttcga aaagaaagaa
    28981 acaggacaat aaaggaatta aattttcctt ttgaaaaata tagaaaagga caaagagatt
    29041 tttcgatagc tgtatattta tcaataaggt caggaaaaaa actttttgca caggcaccta
    29101 cgggagtggg gaaaacagta tccgttttat ttcctgcaat aaaatccctg tatgaaaaaa
    29161 atgctaaaat attctatctt acggccaagt cttcaacaaa gactatagct ttcaacactg
    29221 ttaaaatgat gcataaacag ggtcttaggc tgagaacaac cgttataacc gccaaggaca
    29281 aaatttgctt catggatgaa acaaaatgcg agccggaata ctgcccttat gcaaagggtt
    29341 attatgacaa gcttaatatt ccattaagag aaacaataaa gaataattgc atgtatgacc
    29401 gtgagtttat tgaagaatta ggaaaaaagt atgaactgtg cccttttgaa ctgagccttg
    29461 atctgtccta tatgtcagat atagtaatat gtgattacaa ctattatttt gacccgaggg
    29521 tagcactgaa aagagaggat ctatttaata aggacaagga tatactgctc atagatgagg
    29581 cgcataatct tgaagaccga gtacgaaata tgtattctcc ggagttaatc aaggaagaat
    29641 tttatgaagt atataaaatt attaaatctg aaaaaagctt aagcaaggaa ttatttaata
    29701 ttaataaaaa atttataaat attaaaaaga gtatagatga agcacaaata ttaaaagaaa
    29761 ctccggaaga attaataaaa gctttaagaa aatttatcgc taaggctgat aaatatttca
    29821 acgaaagaaa aaatgaaaaa attccggagg aacttaccga catatatttt aagagtattt
    29881 tttttataaa aatatcggaa attgcggatg ataatttttg ctattatgcc gattctgcaa
    29941 acaataaaac aatggtgaaa ttattcttga ttgatacttc ccaaatattg aaggaaattg
    30001 aaaggaatgc ccggtcatca attttcttct cggcaacttt aactcccttg aaatatttca
    30061 gatatatatt agggggagag gaagatgatt atatattaaa gttaagctct ccttatgata
    30121 aagagaattt aaacttaatg ataacttcag atatatcaat gaaatacaca aagagggatt
    30181 caaatattga aaagacatgt gaatatatca atactttgat aacagaaaaa aagggaaatt
    30241 atatgatatt tttcccctct tataatttca tgaataaggt ttattcggta tatgaatcat
    30301 tgtatgatac catgaacata attgttcaaa gccaggggct caatgaagaa gaacagatgg
    30361 atatcttaag caggttcgaa aatgagcgca atgtggttct attcactgtt gtagggggta
    30421 ctttttctga aggaattgat ttaccccttg ataaattaat tggcgcagtt ataataggaa
    30481 ccgggatacc gcaaatttct tttgaaagaa atataattaa agcatttttt gatgaaaaaa
    30541 tgaacacggg ttatgattat gcatataaat atcccggctt caacaaagtc cttcaatcag
    30601 caggaagagt aatacgaact gaagatgata ggggaacagt cctgttgatt gactcaagat
    30661 tttgtcaata tacatatttg aaattatttc ccgaccactg gaaacatttt aagaaaatta
    30721 agacttcaga agatataaaa aatttgttgt agtataggag gacatatgaa tatattatcc
    30781 gataaaaatg taatgaacaa tcaaattatt acaaaggaag aaacagagct tcttataaat
    30841 aagactaaaa tttatgaggt agtaagggtt attgatggaa agcctatttt tttaaaggaa
    30901 catttccaac gtatgagcga aagcataaat ttaagcggaa tagaaggaaa cataaattat
    30961 gaagagttca aaaattccgt agaattgctt ataaaagaga attccttcaa aaatttaaac
    31021 attagagttt cttactttta tgataaaaag cctcttactt tgttctactt tattgaaagt
    31081 tactatcctt ccaaagacga atttgctgaa ggagtacata ccgttacggt gaaaatggag
    31141 agagcaaacc ccaatgttaa gtcattccaa aaagaatata aggataaagt tacaagaata
    31201 attaaagaaa ataatgcttt tgaagccata ttaataaatc atgacaacac ggtcagcgag
    31261 ggaagcaaat caaatatttt tttcgtagaa ggcaataagc tgataacatc gccggattct
    31321 tctgttcttt tgggcgttac aagaagcaag gttataagcg tttgtgaaga aaattcgata
    31381 gaggttcata aaaggataat tagattggac gagatagaaa gtttcgacgg cgtattctta
    31441 acagggacat caaatgatgt acttccgata aaaaccgttg ataatattgt ctttaattct
    31501 tcagaaaatg aagttgtgaa gaaggtgtcc agattatatt taggagaggt taaaaaagag
    31561 attgagaagg gttaggtcat aaacacaaaa atggcctggt ggccattgtt gtgaagcatt
    31621 aatatgcata aaaggggtat tgggaatata tttggttatc gggggggata accaataaca
    31681 attatagcaa acataaaatt attatgcaag ctttttttac aaatttcgag ttccctaggt
    31741 gccttctcca ggtgcctggc acctcagcat ggtgcctggc accgttagat gaagggcttg
    31801 tcaaagaatc ttaaaagaaa cttataaaaa taaacaaaga ggtaaatatg ttagtatatt
    31861 tggataacag tgcaacgaca aaagtaagag atgaagtatt gaaagaaata atagatgctc
    31921 atgaaaatta ttacgggaat ccttcttccc tgcacaggat gggacataag gttgaaaaga
    31981 agattgatga agcaagaaca ctttcggcaa aactcatagg tgcaaaacct gaagaaattt
    32041 attttaccgg cggaggaacg gaaagcaata atattgcaat ttttggacat ttatcaaacc
    32101 taaaggagta caataatgtt atcaccacta atattgagca tccttccgta tacaatgttt
    32161 ttaaacagtt agaagacaga tgtttggtta agtatttaga aacggatgaa aacggaaaaa
    32221 ttaatattga tgagcttcat aatatggtgg atgaaaatac cctcctcata tcaattatgc
    32281 atgtaaacaa tgaaattgga gcagttcagg aattgaatga aattacggca attataaagc
    32341 gaaaaaacaa aaatgcaaaa ctgcatgttg atgctgttca atcctatggg aaaattaaaa
    32401 tagatgtaaa taaaactcct cttgatacct tgtcgttcag cagccacaaa atacacgggc
    32461 ctaagggagt aggcggatta tttatacgct ccggtataaa aataactcca ttaatttacg
    32521 gaggaggaca ggagagaggg attcgctcgg gaaccgagaa cacaacagga attataggct
    32581 tcggcaaggc ttgtaaatta atgtatgata attttgatga ggaagtaaat aagctttacg
    32641 gtttaaaaaa tacatatata aaaaggcttg aagaaattga agatattaaa ataaacagtc
    32701 ctatagacgg agctcctcat attattagtg tatcatttaa aaatgtgccg ggagaagtat
    32761 tggttcatta tttggaagat aaaaatattt ttgtttcaac cggctctgct tgctcttcaa
    32821 aatcaaaaga cagcaggata ttggaagcaa taaaactaag caaggaatac cgggcaggaa
    32881 ctatacgcat cagtcttggt cattttaata actttgaaga agtcgattat gtaattgata
    32941 atattaaaaa atcggttaaa gatataagaa gtataacaaa gagaggataa tatgtacaat
    33001 atagctgcag taagcttcgg agaattattt ttaaaaggaa aaaacagagg aaaatttgag
    33061 caaaagctga ttgagcaaat caaatatgct ctgaaagaat ttaatcatgt gtccgtatac
    33121 aaagattcag gcaaggtata tgttgaaacg aaaaatgaag aggatatgga taaaatagtc
    33181 gaaaaggtaa ggaaaatttt tggcatatcc aatataagtc cttccgttaa aacaagcaag
    33241 gatgtagata taattattga aaaaacaatt gaactgttta gatatctgtt aagtaaggag
    33301 gatataaaaa catttaaaat taaaaccaag agaagcgaca aggaatttcc ccttaaatca
    33361 atggatttca gtgccttagt gggcggtaaa atcttggaga atttcagctt tgtaaaagtt
    33421 gatgtccata cccccgatgt ggaagtatat attgatatta aaaaatgctg ctatatatct
    33481 tccgaaagaa taaaaacatt gggcggtctt ccccttggct cgaacggaag agcactgcta
    33541 ttactttcag gtggaattga cagccctgtt gcaggatata tgattgcaaa aagaggtgtt
    33601 gaaatagatg cactttattt tcatacatat ccttttacgt ctgagagagc aaatgaaaaa
    33661 gtaattaagc tgaaacaaaa acttgaagaa tattgcggga aaataaaatt atacagtatt
    33721 aacatgcttg aaattcacaa agcaataaga gaattttgca aagaagaaga aaccactata
    33781 ttggcaaggc gttttatgat gcgtattgca gaaaaaattg cattggaaaa taaaatcgat
    33841 atgcttatta cgggagaaag cctgggtcag gttgcaagcc agacaatgaa gtcaatggct
    33901 gttattgaag atgcaatcaa tatgcctatt cttaaacccc tggtgggatt agataaatct
    33961 gaaataatag aaattgctcg agaaataggc acctatgaga cttcaatttt gccatatgat
    34021 gactgctgct ctgtatttgc tccacagcat cctttaataa atcctaagct tgagagtatt
    34081 aaaaggtctg aaagcaattt aaatgtcgaa gaattaatcg aaaaagtctt attaacttta
    34141 gaaattttat aataatgaaa cttgtcattg ctgaacaaca tttttgtcat tctgagagct
    34201 tcagctcgaa gaatctcatg agacattcgc tgggctcagg aagacgaggg ctacgtccat
    34261 gagttgaatt ccaaattatg caacaaatat taataatata ataattaaaa ataataaaac
    34321 gtttcagtat tatcacatat ttttttatct gacatatatt gaattataaa ttaagagagg
    34381 tgatttatat gtcagatgaa gtaagagcaa ctaatttcag atgctgcgat gacagatcta
    34441 atgattggtt cgaatggtta attatattgg ggattatatt tttcttgtta ggaggaaaca
    34501 atttcttcgg aagaggattc ggcagatagt acttactgaa atgcggcttt agaggccgta
    34561 ttttttttat cccttgattt tctattatat aggttgtata ctaatcttta atatagagga
    34621 ggatgttgac aaaatgttta aagtagcact tttttgtgac ataccccagg aaactttaaa
    34681 tttgcttcct gaggataaat attatttaat aagagaagaa acatcagaag ctgacggaat
    34741 tataataaga agtttggata ttcatgatat gaagttttca aaacagctca aagccatagc
    34801 caggtcggga gcaggagtaa acaacattcc tgttgaaaga tgctcagaag aaggcattgt
    34861 cgtattcaat gctcccggag caaatgccaa cgcggtgtgc gagttaactt ttttaggact
    34921 gttaatgagc tcaagaaaag taatagacag tatagattgg acaaagaagc tggaaagcaa
    34981 cgtcgttgaa accgtggaaa gggagaaatc caaattcgta ggaccggagc tgaaagggaa
    35041 aactcttggg gttataggac ttggaaatgt gggacatctt gttgcaaacc tggggactaa
    35101 actaggtatg gaggttatcg gatatgaccc ttatatttca gtaaaaaacg cactctcatt
    35161 atcctgggat gttcaatata ccggtgatat taatgattta tataaaagaa gcgattatat
    35221 aaccattcat gttccttata ataatgaaac aaaggactat ataaataaag ataatattaa
    35281 attgatgaag gacggtgtaa aaatattaaa ttttgcccgt cacggaataa tcaatgagga
    35341 tgatttaata gaagctttag aaagtggtaa ggtaagttat catgtttcag acctgccaaa
    35401 cgaaaaactt ttaaaagcaa aaaatacctt gtgtattcct catctgggag gctctacaat
    35461 cgaagcggaa gaaaactgtg ccgaaatggc tgttacggag ttgacagaat acttggaaaa
    35521 aggcaacatt acaaattccg ttaattttcc cgactgtgcc atggacaggt atccaggaac
    35581 ggataggatt gttgttttga ataagaatat tccaaatatg ataggtaaaa tgaccactgc
    35641 acttgcatct cagaatgtta acataatagg gatggtcaat aaatcaaaag gtgactatgc
    35701 ttgtaatata attgacgtaa tcggtgagct caatgaagat atgttaaatg gattagtaga
    35761 gcattacggc atactaagag taatgcttat taaaaataag aggtccaaaa aatgaaaaaa
    35821 gtcaacatat atacggatgg agcctgcagc aagaatccgg gtcccggagg gtatggtgta
    35881 atattggaat acaaaggaca tgaagttgag ctttcgggcg gttcagaaaa tacaaccaac
    35941 aatcaaatgg aactcatggg agttattata ggacttgagg cattaaaaga gccctgcgat
    36001 gttacaatcg taaccgactc aaagtatatt gctgattcct tcaataaagg atggattgat
    36061 gcatggcaga aaaagtcttg gaagaaatct gacggtaaag cagttttaaa caaggaatta
    36121 tggcaaagac tattaaaggc aatggaaggc cataaagtaa aatttgttca tgtgcccggg
    36181 cacaaagggc acatctataa tgagagatgt gataaactag ccgtgcaaga aagaaaaaaa
    36241 tttgaaaaaa agtaaagatg cataatgtgg aaacattcac tttatcatga atattataaa
    36301 ctaacaaatt ataatatgag gtgattatag tgatttatag acaaaatcag caattatgcc
    36361 ctaatatata ttcaatgcca tacgaggtgc gacccggaga tacgttaaat tcaatagcag
    36421 caagatttgg tacaaattta gtagggctga ttgaattgaa tcctacatta agattaaggt
    36481 cattaacgga aggaatgact atatgtgtgc caagggttcc tgaaagaccg ccatgtgtta
    36541 atggagccta ctataccata agggaaggag atagttttta taatattgcg caaagatatg
    36601 gtattccttt gaatttgctc ttagaagcca atccggaagc aaatccgtat gatttgaaag
    36661 ttggtcagga aatatgcata cctgatgttc ctccggggtg tcctttcggt acggttgtga
    36721 ctatacaaga aggaacaagg ttaagtgata ttctcatatc ttacaattta agcttaaatg
    36781 aattaagaga tgcaaatgtt aatttcaatc ctaatattat tgtacccgga actgaactat
    36841 gcatacctca ggaaacattt atgcagtgtc ctgaaggaac aaccacagaa tacgtaatac
    36901 aggcgggaga cagtctttct acagtggcaa ttgcaaataa tattacacct tcagaattac
    36961 ttattgcaaa tcctcatttg agaccggcaa actttctgat tgtaggaaca agagtgtgta
    37021 taccgacagc acgataatat gaaaatcagc acagcagtga aaactgctgt gttttaatta
    37081 gtctaatatc atttgaggaa tttatcaaaa atattcactt cttttgttgt gattcataaa
    37141 ctatatggag gtgattgcag tgtatgttga aaaaatcgtt acaacaggca tatgtcaggg
    37201 aaaattattt ttgtttataa tggagctttt aaaagaggat tattctttta aaaaagcttt
    37261 atatttggga gcaggcgaca gctttgcaat ccacagcata gaagttttta atgatgcggt
    37321 atatatgaca gattccttta ataacaaaat ttacaaattc gacagcgtaa ataatttttt
    37381 tgaaaccaat gtgggaagag acccgcggca tatgtgcacg gacaaagata atatatatgt
    37441 agcaaatttt gaatcggata atatttcaat cattgaaatg gataccttca cacttacagg
    37501 ctctttacct gcaggcataa aagcacatga cgtaatttat tcagaaaaaa acaagtcgct
    37561 ttacacaacc tgctacgaag aaaatgaagt attggaatac agtattgaca atggaataag
    37621 aagacgattc aaaacggatg gaaagcccat gcatatattt gtgctggatg atacgataat
    37681 agtaatgact tattatgtca atgggattgt acaaactaaa ataaatttca ttaatttatc
    37741 aaataaagaa attgaagata ctatcgacat agatggtctt gcttcagatt ttttactgga
    37801 ttatgaaaac aatatgctgt atataataaa tattgttgat aaaagtcttt atattgctga
    37861 ttccttatta agaaaaatta ttaaaaaaat atatttggga ggatatcctg aatccatttc
    37921 ttgcggcagc aaaaatattt atgttactaa ttctaaaaag cagcaaattg atataataga
    37981 aaaacaaggg cataatataa gcaccttaaa actaatgttt acccccggtt taataaagat
    38041 tataccttaa tttgttaatg cattaataat ttctccgaat attttatcag gattgttttc
    38101 ccatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaataa tccatcatgc
    38221 catattccaa tatataatta gtaatttcat tatttgtcaa aggtatatca atataatcca
    38281 atatatacaa aagtttaagc ttatcttggg tacttttatt gctgcttatc aattttgccc
    38341 tccattaatc atataattat tccggggacg aagactgtca tccccctaag tacagggaca
    38401 ttccttctac aagcaaagac tttccctaaa ataagaaggt gtgtccctct tcatcttttc
    38461 ctattatatc aaacatgcag ttaataataa actaaaattt gaaaatactt catgtagaca
    38521 tttatactta aataatatag tataataata aggaaagagg agggcaaatg aaaaaaataa
    38581 ttattttaat agcattaata atgatgacag gatgtggagc tcccacagca gaaattcctg
    38641 aaaacccccc tgccgtcgaa gctccgggtg aagaataccc tgaagaagat aaagaagata
    38701 ccgataattt aaatgaagac aaagagctaa gcgatgagga aattcttgct tccatcgatt
    38761 tgtcgttgaa ccctaatgag cttggggaaa ttatgatatt gatgtatcat ggaataggtc
    38821 cggaagaatc agtatggcaa agaactccgg ataactttcg caaggacttg gagtacatgt
    38881 atcaaaacgg ttaccgaatg ataagtttaa atgattatgc taagggagaa atcaaaacag
    38941 aagcaggata tactccaata atacttacct ttgacgacgg aaggcaaaat aatttcagtt
    39001 atattgaaga aaacgggaaa attgtaatag atcctgactg tgccatcggg attttggaag
    39061 aatttaaaaa taaatatccc gactttaatg taactgccag cttttttttg ggaactaatt
    39121 tgttcggaca aaaggaatat gcagaaaaaa aacttaattg gcttattgaa aacggatatg
    39181 atttgggaaa ccacacctac agccataatg agatggcaga attaaattcg gatgaaataa
    39241 taattgaaat aggcaccgta aacaacataa taaaacaata tttgcccgat tatgcagtag
    39301 aaacattggc tcttcctcac ggcagcaatc caaaagatga atttatagaa tcggtgcttg
    39361 aaggagaata tgaaggcaat aaatatagga cccttgcagt tctggacgta ggctggagac
    39421 ctgcctattc accttttgat atattgacaa actttagtaa cctctaccgt gtaactgcaa
    39481 gtgaaattaa tgtggataat tgcgggatta acgattattt taaacaatat gaggaaaaca
    39541 aaagagaaag atttataagc gatggcaatc ctgacgttgt aaccatacca aaaagacatg
    39601 tcgactacct taacatggat attgtagggg acagaatagt aaatatttac gaataatgac
    39661 aaggggagct ctgctcctca ggttattggc atactccatt atgtcatcct gagcgtaagc
    39721 gaaggatctc ttcttatagg gagctctgct ccttttttat tgcgataggt ttgagagaaa
    39781 taaggaaata tagtataatg gttaaaagaa taataagagg gagatgttgt tatgatagct
    39841 gaattaaaag aaataatatc aaaaagtgat aatatagtat tttttggagg agcaggtgtt
    39901 tcaactgaaa gcggtatacc tgattttagg agtgttgacg gattgtacag caaaaaatat
    39961 aaatatccgc ctgaaatgat ggtttcccac agtttcttca ggagtaatac cgaggagttc
    40021 tttgattttt atataaataa aatgattttt ttaaatgcta agccaaacag tgcccataaa
    40081 aagcttgctg aacttgaaaa agaggggaaa gtaaaagctg ttattacaca aaatatcgat
    40141 ggacttcatc aagccgcagg aagcaagaat gtattggaac tgcatggctc ggtacataga
    40201 aattactgca tgaagtgcag aaaattttat gatgtgaatt ttattgtcaa ctcaaaagga
    40261 atacctaaat gccaatgcgg aggaatagtt aagccggatg ttgtattgta tgaagaaagt
    40321 cttaataccg atgttataga aaaagcggtt aacttcatag aaagggcaga tgttttaatt
    40381 gtcggcggga catcccttgt agtttatccc gctgccggcc ttattaatta ctttaaggga
    40441 agcaaattgg tactcattaa caaatcatca actccaatgg atagaagagc cgatttagtt
    40501 ataaatgaca gtatcggcaa ggtctttgaa gattaattaa tattggcgta tttacacttc
    40561 ttttacatta tgttataatt aaattaatat aaacttaata actccgagca taacagccta
    40621 agaatgatat ttatggcctt atgccgatag ggtttgattg gaaacggtca agcctcctgg
    40681 tggaaaggag aaactagata atatgtgaac agcaattcca tccagaagtg ctgtttttta
    40741 gttcgtttac ttttctgatt tatatttagt ctaactaaat taaaatataa ctattaggag
    40801 gaatgtatga aaaaatttaa agtaattgca atggtattgg caatagtgat gattctttcg
    40861 caatcattgg tttttgcaga agattatgca attgaaatcg gagggacatt agttgcaaca
    40921 gagcttaagc ttacccttga agatttaaaa tctatgcccg aggaagcgca aatagcacag
    40981 gagtatgttt acaattctaa gggaggagaa aaaacagtcc aggttaaggg tgtaagttta
    41041 gcttatttat tgaaagaaat ggcaggagta actgctgaag aaggaaatgt agaatttttc
    41101 gctgccgatg aatggccgat tgacccacag ccccttcagg atgttctaaa cgatgaactt
    41161 aaatttgttt tggcttatga ggtagacggt gaggcaatta acaatgatga aggaataatt
    41221 gaagaaataa ctgtatacag aaatttaaaa tatgaagatg aatttgatac tgtttataaa
    41281 ctaattaata aaattactgt aggggaagca gaagcagtgg aagaagaaga agcacccgaa
    41341 gaagagacta ttgagccgat tgaagaggaa actgaagaaa cagaagaaac agaagaagta
    41401 acattcactg atataaccga agaatataaa tttgcagagg cagctattta tgacctcgcc
    41461 aaaagaggaa tcattgatgg aatgggaggc ggactctatg ctcccggaaa tgaatttaca
    41521 agagaacaat tctgcaaaat tatagttgtg gcattggaat atgagctgaa agaatacgaa
    41581 ggagcattca gcgatataga tgatgacaga tggtcggcac catatgtaca agctgcagtt
    41641 gattcgggtt tgtttgtagg atatcctgac ggtacatttt tacccgaaaa ggtaataaca
    41701 agacaggaaa tggcacttgt agcagccagg gcagcagttg caaaaggatt ggttgaacaa
    41761 gcaaaagttg ataaatttgt aatggaaaaa tctgattaca tagacaaaga agatgtggca
    41821 gactgggcag gaaatgcagt tgcatggctt gaagcacagg atgtgtttgc agggattgca
    41881 gtagaaaaat ttgagcctgc aaggaatgtt aatcgtgccg aagcagcctt ggtagtattc
    41941 aatacattgt tttcggaata atcaaaataa atgagcgata ttcgctcatt tattttaaag
    42001 gaggtaaggt aaatgacaag aaaaattaca gtctggttct tgattgtatc catgattata
    42061 atgaatgcaa cgtttgtatt tgcagcagag cctgaactta taattacagg cacgggagtg
    42121 gaggaagaag tagtaatctc atccggtgac tggtcaaaat ataagatggt agagagaatt
    42181 tactccacca acaacagcct tggatttcat aaaatcataa aggcaaaagg atatgattta
    42241 tttgaactta tgggaaacgg tttgaaaacc gataaagact acagcgtaaa atttacatgc
    42301 tctgacggtt ttgaatttac aaagactatc agcgagctta aaagcgctta ttatttcagt
    42361 gattttactt tggaaagcaa acaggcagtt atgcctatgg ttgcaaaata tactacggta
    42421 atggctgatt ttccgaaaaa tatttttgct cctcctctta catggacgga tattcccatt
    42481 acggaaaaag atttagataa agattttcca aaacttgtat tcggacagac tgacattgac
    42541 gatatgaata tgtctaaatg gggcaaggaa gttataaaaa ttacggtagg agatgaaata
    42601 gtaaaggtaa ctccttcatc accatataaa catatcagct atgaaggtga gccttacaat
    42661 gtggatgccg taacaggagc aacttttaca attgaaggac ctgcagttga aggttacaga
    42721 gccatatcat taaggcaaat agaagaagat acaactggac agacaataga tacatattat
    42781 gaaaaagtaa acggtaaggt agttgaagtt tcctatgaag gaataaatgt caaatatcta
    42841 atcgataatt acgtaaaggc aaagtctaat gccggaaata ttatatttaa agataaatca
    42901 agaaagacaa ttataaccgc cgacatatcg gaggcaggca actacatggt tgcatacggt
    42961 ataaatgaag ttcccctggt ttacttggat actgatgaag gctatatcaa ggaaaaacat
    43021 aacgataacg gttgttttaa attagtttat gaacaagaca agaataaagc cgaggaattc
    43081 tccaatgtag catatattta catagaagaa aaggatgcca aaaatatttt tgagcacagc
    43141 tatgctcctt acaatgctga aaaatataca gattatgaat tgataattca tggttctgct
    43201 ttgggaaaag aagtaagata caaggtaaaa gatatagaag caatgacaga ccttcatgaa
    43261 acacatgaat acagtttgtc aaacagtgaa tatttctggt attataacac atacaagggt
    43321 gttcctttat gggatttact cttaaaggcg ggtttagacc ctgacattga cgaaaataca
    43381 agcgtacgat ttattgctgc agataattat aattttgcac caatgactat caaagagatt
    43441 aaggacaggt ctctgtacgg atattatgaa aaaagtgcaa tggataaagg tgacgggacc
    43501 ttcccgggag aatatgtaga tcctctttat acgaatttac ctgttatggt tgcatatgga
    43561 tttaacaaat atccctatgt aataaggcct accgatgaag ggtacaaccc aggtctcggc
    43621 aatgacgggg gacctcttcg aataatattc gggaaaacaa attataacga tacgaacggc
    43681 tccaatcagg tacaatttgc aaaggaaata attatcggcg aaggtgaatc tcttgcaaca
    43741 gatacccaag gaattggaga aggagtcagc acggaaatag aaatcgatga atcatggaat
    43801 cacaatcaag gtgtatataa ggaatatttg gatatgccag tactgcgagt aacaggctca
    43861 caagttaagc aacctatgac atttacctta aggcaaattg aggccatgac tgaatatgcg
    43921 gttagagata cctatacagg cgatggcata agagaaatgc aaggtattgt tttatgggat
    43981 ttaataaata atgtggtagg gctcaaagaa gatgtagaag ttccaagtat aagagtattc
    44041 agcggacaaa attacaacca aatattaaga agcaacgagc aggtagtaaa cggagtctta
    44101 aattctcaag gtcagactaa gaaaataatc ctagcttatg cggcagacgg gtatccctta
    44161 gtacccgacg agtcaagtat cggatatgta aacaacaatg catacggacc attaagactc
    44221 ataattgaag aaagcaaatc aatgtgggtt aaatggactg actgtatagt agtaggtaca
    44281 ggggattatg aagcacccga catgaaggat gtaaaaattg atactttcag tgatattgaa
    44341 agccactggg caaaagacag cataatagaa atggcagcaa tgggttatgc aaaagggagc
    44401 gacggtttat tcagaccaaa cgataatatc agcagagcag aatttgtatc actgcttata
    44461 agggtactgg gtatgggcaa tgaagatgca atacctaaat ttaatgatgt gaaagctacg
    44521 gattggtttg ctaaggatgt tgcaatagca gcagaaaaag gatatgttaa aggctatgaa
    44581 gacggcacct tcaaaccgaa ttctcctatt tcaagacagg aaatagggac tataattggc
    44641 tctttgctag aagatgatat aacaaatgct catgaaattt tgtctatttt cagcgatgaa
    44701 gttcccgagt gggcaaaggt acccgttgca aagacggtaa aggctgaaat tatcaaaggg
    44761 ctgcccggaa atattttcgg cggtgcacaa aatacaacaa gagcagaagc agcggtaatg
    44821 ctgttaagat atttagacaa atgaaaaaca ttttgtcatt gcaatgaata acaattttgt
    44881 cattctgaga gctttagctc gaagaatctc atgagatcct tcgcttgcgc tcaggatgac
    44941 aaatatgttt gatattttaa cagataaatg gttaaggaaa tatttgaggt gtatgatatg
    45001 aaaaaattat taattatttt atttgcaata cttttgatat cagcaggatg ttctcaaaat
    45061 gcagctgatg ctcccgcaac aaatgaaatc actgaatccg gtgaaaataa taatgaagta
    45121 agtaaagaaa ttggaaattc aaatgagccg gaagcagaag aagatgaaaa gactgaagaa
    45181 cctctaagtg aaaaaccttc tgaagaagca gaagaggcag caaagaaaga agaaccctcc
    45241 gaagaaatat ccgagcagaa agaaccgata actgaagaag ttttagaaga caacccggca
    45301 gaagaagaga cggaaattac agagaatgca ctaataatta aagggcatgc agaaaaggag
    45361 ctttctctta cattagatga attaaaggca atggatgata ttatttttga agctgatttt
    45421 tattcattaa ataatttcgg tacaacgggt tatacgcatt ttaaaggcgt taagctgtgg
    45481 gacctgttga aaaatgaagc attgataaaa cccgaagctt ctaaaatatc aattatagcc
    45541 caagatggat acagcatgga atttactatt gagcaggtac aaatggaata tatggatgaa
    45601 acaaatcccg aaaataaata tccaatgatt attgcatggg aagaaaacgg agaagaatat
    45661 agtcttgatg aaggtaatcc gtataaattg gtggtaggac aaaaagagcc cggagatatt
    45721 aacaaacctc aatgggtttc aaatatagat gtaatcctta ttgaataggt gaaaaaatga
    45781 aaaacagata cattatcatt atttttatta ttcttgttat tgcaggctca ttattttatg
    45841 tattaaatga ttcttccgag gaagaagaag cagttaggtt attttatccg gatgcaaaaa
    45901 gagtgacact cataaaaggt attaatgatg atttatacag ctccttatat tttcctgcag
    45961 ttaaaagagc ttatgaagta gacggtgaaa tttccgcata cgttgtaagc tgtgtcggat
    46021 ataacggacc tgttgaagta ttggctgcaa tcgatgatga taaattaatc ggaataaaaa
    46081 ttttaagcca cgaggaatcg cttgattatg cagagcatat agaatatgat tactttttgg
    46141 acaggtttaa aaatctgcct attaataaat acttaaactt ggttgtgctg gataaggaaa
    46201 atcctgaaga tattatccag gttacgggag ccacaataag ctctcaagct gttgtgaatg
    46261 ccgttaatgc cgcaatcggc tcttatatgc tttggaatta cgatattcaa atggcaaaag
    46321 ttcccgatgt agtacctcag gaaatgtggc agaaggatat aaacagcttt gcaattaatt
    46381 gggaaggcgg ctctattcgg atagatacag atgaaatcaa agaatatgaa cagttggaaa
    46441 tggatgtaac cttaataaat acaaccggaa cagaaacaaa aatgcgtgta aaaggtccta
    46501 ctcttcgcca tgtgcttgaa aaagaaggta ttgatttaag tgagtatgcc ggaatagggg
    46561 taacgggaag agacgggtac tacactttaa ttgacaagga aaaactggca gtaaacgata
    46621 taatacttac ctggcaagta aacagaaaga atatcaaaga tgaagaaaaa ccgataagaa
    46681 tttccgttcc tttagagctt gggccttact gggtaaaaat ggtttcaaat atagatttat
    46741 acaaggaaat atcccccaag gatattgata aagtacatat gttcaatcca ttgactgaag
    46801 atattgagcc atattattat gaatactacg gctctaagga taaatccata gaggtcggaa
    46861 aaatattaag aaaatttgat gtggttgatg aaaagggctt ttttaccatg gctgccacgg
    46921 atggtttgga aaaacatgaa acaatatcat tggtaaggca aaggtatttt ctaaaggtag
    46981 aaggtgataa tgctcctatg aatattgcac cgaatttcaa gcttggaatg aatgtaaagc
    47041 atatgactca cttttctact acaaaggatg ccgtaatttt tcccgaaaag atgattgaag
    47101 ttgtaagaac taaatcaatt gaaggcaatg atggaatgct tttggaagat gtgcttttga
    47161 cagccggaat gagatgggat gaaggcaaca aattcactgc cgtaaacaag aataaaaaaa
    47221 tagatttatc attggaagaa atgttgaatt gttacttaat ctataaagaa ggacaagtaa
    47281 gcttatataa tgataaagaa ataatgactg aactttcaag gatcgaaaaa aaatgaaact
    47341 tagtataaga agcattatac aaattaccgc aactcttatt gtgataatat catgtttgat
    47401 ttacggcatg tatataaacg aaataattga atttagaatt ctctccatag cagatttgaa
    47461 tccatacggg ggatggagtg ctttgaaatc tttatttacg gatttatctt acagatggaa
    47521 ggggttttca aggagtattt ctttaacttt aggaatagtt gccgcttcat ttttaatggg
    47581 aagatttttt tgcggatata tttgtcctat cggagcaatg caggattttt ttaaatatat
    47641 tggacgcaaa ttaaggttta aggaagtaag attttctaat aaacctgaaa ttcttaaata
    47701 cattttttta attgttataa tggttttaag tatagtcggg atgggaaata tagtgtcccc
    47761 tctttcaccc tggcttgcat acttaaatat atttgccgga ttaaaacttc aaacaggagc
    47821 ggtaattttg ctttttctta tctttgtatc attattcggg aggagaatat tctgcaggta
    47881 tttttgtcct ttgggagctt ttcaaagcct catatatgca ataggaccgc tgaaaattaa
    47941 aaaaggaagc tgcaattgct cctattgcct gaaaaattgt ccggtatcag aagagtttag
    48001 aagaactgaa aatgaaatat ctcctgaatg cataaattgt ttaaaatgta ttaattcatg
    48061 cattaaaaac aaagaaggct tttctataac ctttatgaat aaaaaaataa acagattaac
    48121 ttatatagta atatctatct ttattattgc aggttcatat attttacttc ctttgacggt
    48181 atcaaattct tcggtacagg caataacatc aattaccggt ataaatgacg gtacctatga
    48241 aggatttgga tttggttttg gcggattaat aaatacggaa gtaaccatta acaataataa
    48301 aataacagat ataagaattc taaatcacag tgagacagca ggttattacg aagaagtatt
    48361 cagggaatta gcctatgaaa tcaccgaaac tcaaaattta aaccttgatg ccataagcgg
    48421 tgctacatca acctgcaggg gtttcttaaa ctccttaaag gatgcagtaa acaaatcctt
    48481 agctgattag aggttattat gcaaaagaag tttattattt tgattatatt aattatagtt
    48541 ttatcgggct gctcaaaaga aaagccttcc ttaaatttaa atattgacgg aaaggttgaa
    48601 tcaataaata ccgaaattat ttatacccat aaggaagctc ttgtatttcc tgcagttgta
    48661 aggagcagcg gttcaaaacc tgttgagacg gaatataaag gaattgaact tataaagctt
    48721 tttgattctt taaacgtaga tataacaagt tacagcaaag taaccttcaa tgcaagtgac
    48781 ggttatagaa taattcttag catggaagaa attaaagagc cttcaaatgt ttatttgact
    48841 tttgagaggg atggagaaat tcttaagtca aagaagcaag gaggaaacgg acccttccag
    48901 ttggttatca gaagagaccc cttcagtcaa cgctggatta agcatgtaaa cgaaataata
    48961 ttagagtagg tttttatgac aaataaattt ttggaaaaat tttcagttta taatttaatt
    49021 attatagcag tggtatcggc actcggaatt gcaacaaaac caattgtggt gcccttggtt
    49081 cacattataa cgggaccctt gtttattccg ggaggagcaa tagccggcgg tttctatatg
    49141 ttttggatag tcttgggagc cggaattgtc aaaaaatcag gcacgggtac cttaataggc
    49201 attgtccaag gaattttggt aattgcaacg ggaactatgg gaactcacgg catcatgagc
    49261 attgtttctt acactctgcc cggtattgtt acagatataa tatttatgtt ttcaataaat
    49321 aaaaaataca atattctcca ctatgtgttt ggctgtttgg cggcaaatgt tacgggtacg
    49381 cttatttcca atatactatt tttcagactt ccctttgtaa cggttatttt aatcttgtct
    49441 gcagcagctt tgtccggagc aacaggcggg cttatagctt acgccattaa cagaagtttg
    49501 gaaaagatga aaatattaga ggattagaaa tgaagctgaa aaaaatattg tcggctatta
    49561 attatacaga acatagtctt gggattaaac tatttttatt aatatttttg gtaatcttgg
    49621 ttggatgcag tgctgatgta aactataaag aatcaacagg cataaatata ttcaacgaaa
    49681 ataaaaattt tttcgttgat tttaagtctg aaaaagagat ttcaataaac gaaataacaa
    49741 acgataaatt ttcagaagca gcggtaattt taaatacagg agaaattatt caagtgcctg
    49801 ccgaattcta cataaaaaat aatacagtta cagctgatgg ggaaacagtt gaaaatacag
    49861 taggaattta tttaaccgat aattatcaaa gcataacaaa tgcatattat gacatgaaag
    49921 aatatttgga taaaggcgaa aaggttatga cggttctttt agacggcttt tcctacaatc
    49981 agtttaaaag ggctaatgaa gagaatactc ttactttttt gagcaaatat ttccaatatc
    50041 ctgccttaag tgtatacact cctgttacaa atgcaggatt tgcagcaatc attacgggac
    50101 aagcccctga tgtaaacgga gttcatgaca gaagtaatcg ccggatgaaa gtagacagca
    50161 tattcaaata tgctcttgat aacaataaaa gctgtatttt gcttgaagga gatataaaaa
    50221 tattaaatac cgaaattgag cctgaacttc atacagatat taataaaaac agcgagaccg
    50281 atgatgaaat atacgaaagt gcattaattt ccgttaacga agactatgat ttgattttta
    50341 ttcattttca cggcattgat gacagaggcc acagttttgg accatattca agagaagtga
    50401 tggattatat aaaaactatc gataaatata taaaagactt aagtgaagag tgggagggag
    50461 caataatact tgtgcccgac cacggtatgc atgaaactgc cgaaggagga ggacatggaa
    50521 tatgccgtca atcagatatg gttgtgcctt atttcattaa agagccatga aaaaaagagc
    50581 tttaataatt gtattattag taatcatatt gacaattaca ttaataatat ctttaaactt
    50641 aaacaaagcc tccatcgaaa aacaaaaaca aatattatct aatgcgcaga ttgtattgaa
    50701 atataacgac aaagagaaag tattaacgaa ggaagaaata attctttacg gtgaggactt
    50761 tgaagctgtt cttgacacat cgactactga accgtcaacg catatatata ggggtgttca
    50821 gctgaaagac ttgcttaata accacaatat tgatttacag gacaaaatta ttataatcaa
    50881 ggcggccgac ggctattctg ttgcttattc atcggaagaa gtttctattg ataaaaatgt
    50941 atatattgct tttatggaag acgggagata tcttggcagc agggactcgg gaggaagagg
    51001 tccttatgaa tccatagtag tttcggattt tttcagcaac agaagatgca agtggattac
    51061 tgaaatcgag gttaaatgat gaatgcagtt gaagttaaaa atttatgctt taaatatgac
    51121 aaccggaaag aatatatttt taaggatgta aatttcaatg taagaacagg tgaaacaatt
    51181 gcaataacag gacgaagcgg aagtggcaaa accaccttgt gccattgtat ctgcgggctt
    51241 gtgccgagat tatacaaggg ggaattaacc ggagaggtct tgattttcgg tgaaaatata
    51301 aaaaatttaa gtatcgcgga agtagtgaca aagataggaa taatatttca aaatccttca
    51361 acacagctat tttctcctac gattgaagat gaacttgcat ttggtcccga aaatctatgt
    51421 gttgaaagag aaaaaataag ctctaggatt gatgaaatat taagaattat aaaaatggaa
    51481 aaatacagat atgacaatcc gaacaattta tcgggaggtc agcagcagct aattgcaatt
    51541 gcatctgtac taatgcttaa accttcaatt ctcatatgcg atgaaattat gtcatggatt
    51601 gacgatgagg gaagaaaaat aatcaaagat cttttactga agttaaaaaa agaaggaaaa
    51661 acaataattc tggttgacca tgacaatgat aatctaaata tagccgatag gattatacat
    51721 ataggagaat aatgaacaat aaaattgaaa tcaacaatat ttcatatgaa tatccgagag
    51781 gaagaaaggt atttataaat ttctctctcg aattaaataa aggggaaaca actgttataa
    51841 cggggaaaaa cggttgtgga aaaacaacct tggctaagtt aattatggga atattaaaac
    51901 cttcattcgg ggaaataaaa ctattcggtc aaagcacaaa agatatgtcc cttggagcca
    51961 taggtgaaat tataggctat gttttccaga gtcccgaaag gcagcttttt ggcactaccg
    52021 tattggaaga attgacattt cctttgttgt ttaaaggata caacagggaa gaaactatgg
    52081 atagggctga aaaaatgctg gaaatttttg atttgaaaaa agttagaaat tcatacccct
    52141 attttttaag ttacggagaa aaaagatgtt tagcaattgc ttccgtattg atggttggtc
    52201 ctgaatatct tattttggat gagcctacgg cttctcttga taaagaaaga atagaggcat
    52261 tatcgcaagc tcttgaaaat ctaaaaaaaa ggaatatcgg gatgcttata ataagccata
    52321 acaaggattt tatcgaaagg cataaagaca gaattattaa tcttgaaggg ggaagttatg
    52381 aataatatag acccccggac aaaattaata caagttttaa ttctatcaac ccttgcatta
    52441 atttataatg aaataacact tttatcaatc atacttattg ccgcaattat gattgcactt
    52501 attaacaatg taaattttat atcagtaatc ataagactta ataagttatt aaaaataata
    52561 ttcttgattg cgttaattca gagtttattg acaaatatgg gaaatcctat ttttaaaatg
    52621 ggtaatttta cgctttttac cgattacgga attttaaaat catttgaatt tattttgcgc
    52681 atgggaataa tcatagtttc tgctgcaata ataacaacat catcaagcag ggaaataata
    52741 cagggactta ttcagtggaa ttgtccttat gaaatagcat tcatggtttc tgtcgcagta
    52801 aggtttttac ctattttcaa agaagaaatg acagatataa taacggcaat acaactaaga
    52861 ggaattgatt tgaagaaggt taaacttagt aagaaatttc aagtttacaa atatatactt
    52921 actccaataa ccgttaattc gattatgaag gcaaaggaat tggctgcagc aatggagatg
    52981 agaggtttta gagcttactc cgaaagaaca agctatttgg ttttaaaaat gcagaaattt
    53041 gattatattc tattagggct gagcatttca gcagcattat tgtttattat tataacgaga
    53101 ggtgcattat gaaagttttt tcagtaatcg gactgacaaa atccggcaag actactacca
    53161 tagaaaatat aataaaggaa cttaggaaac gtaattacac tgtcgggagt gtaaaagaaa
    53221 tacattttga gaaatttaaa atggatgttg acgggactaa cactcacagg cataaaatgg
    53281 caggctctca attggttacg gcaagaggcg cctatgaaac agacgttttg tttcaaagaa
    53341 aactaagtat gaatgaaatt ctttgttttt acaatcatga ttttgttatc ttggaaggag
    53401 taagggatac atgtgctccc aaaatagtaa cagcccatga tgtggaagga ataatagaaa
    53461 ggcttgatga aacaacattt gcaatatcag gacaaatatc caacagttta acggaatatg
    53521 aaggtatacc cgtaataaat tcgttaacgg aaattgaaaa gctggtagat ttaattgaaa
    53581 aaaaagtatt tgacagactt ccggacatga aggatgaatg ctgtaaaaaa tgcggatata
    53641 catgcactca attatgctca aagattttaa aatgcgaggc ggaaaggagg gattgcgttt
    53701 tggctgaaaa cagtgtaacc cttaaaatta acggacagga aattgctatg aatccgtttg
    53761 ttcagaatat tttgaaaaat acagtggaag caattgcaaa ggagctatac ggttatacgg
    53821 aaagcggaga tatcgaagta tgcataaaga gataaaacta agaagcaaaa gaaataatgt
    53881 atttcgaata gtggaagatg aaggaacata tattctgaaa aaatttgaga accacgaaaa
    53941 ttacataagg gaaaaagaag tgctaagtat tttaaaaaaa gcaggagtta atgttccttc
    54001 aataattaaa gcagaagaga actatctata ccttgaagat ttgggagaag ttaattttct
    54061 tgaatggtat gaggaacaag aaaaaaataa tgctttgaac ataagcatgg tttacgagct
    54121 ttgcagttgg tttaaagatt tttattctgc agtttttgag ttctacaaaa aacaatttat
    54181 attatatgat gtaaatttta aaaattttat tatttgcgat aataaagttt atggaattga
    54241 ttttgaacag gtaaagccgg gtcatataga agaagatgca ggcagattat cagcttttgc
    54301 attgacttac aacccttcta tgacagaatg gaaaatggat tttaggaata ttttaattaa
    54361 cattttatct aatgaattaa atattgaaaa agaaaaaatt atttcggaag aaaacaagga
    54421 attggcagcc attaaaaaaa gaagaggtgc tttttcttga ggtgactatg aaaagcctat
    54481 caattttaat aataatttta ataatttcta ttgtcggatg ctcatcggat aaagaaataa
    54541 ttgaagatat aaatgaagaa gtattagaaa tcagcaaaga tggtatatcg gaagatgaac
    54601 aaacaattga atactctgaa gaaattcatg aagaaaatca acaaatacct gaagaagaag
    54661 agcctttgcc tgaattatct gcaaaagagc ccttatacga tgaagaatcc gaaaatgata
    54721 tgacttctgc acctgccgat gagccattag cggagttgga acag
//
LOCUS       DAOSHP010000003        48465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_560_length_48465_cov_9.435199, whole genome shotgun sequence.
ACCESSION   DAOSHP010000003 DAOSHP010000000
VERSION     DAOSHP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 48465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48465
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_48465_cov_9.435199"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..843
                     /locus_tag="PLB33_00640"
     CDS             157..843
                     /locus_tag="PLB33_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG62057.1"
                     /translation="MKVLIVDDELLTIKNLKYSFEQDNYEVESTGDSNEALKKITTTD
                     YDIIILDLVLPQTNGLEVCQKIREISNVPILILSANNDDMSKILGLEYGADDYITKPF
                     NILELKARMKAILRRTRGTSQKANEQTLVVDDFIINALGRKVSLHKQEINLTAKEFDL
                     LLLLITNPGKVFSREELLEIIWGYEYYGDLRTVDVHVRRLREKIETKNKAYEYILTKW
                     GVGYYFRNRN"
     gene            957..1051
                     /locus_tag="PLB33_00645"
     tRNA            957..1051
                     /locus_tag="PLB33_00645"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:991..993,aa:Sec,seq:tca)
     gene            1069..1437
                     /locus_tag="PLB33_00650"
     CDS             1069..1437
                     /locus_tag="PLB33_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG62058.1"
                     /translation="MPNCVLVCVTRQKTSEKLIKKGVELIKGEAGSCLNVIHVVNEND
                     KFLYGLSDGDALEYLFEITKDVGAANLVVKRSKDVIKTIVEYAEEINCTHIVLGHSNN
                     PSNNFIAKLQRKLPNVEFII"
     gene            1447..1728
                     /locus_tag="PLB33_00655"
     CDS             1447..1728
                     /locus_tag="PLB33_00655"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOG62059.1"
                     /translation="MGRILYIIKFLFDKNIPIREKWWVIIPLIYILSPADLIPFPILG
                     LGIVDDLVMLGFLLTIVYDKTKKYYFDKRHEDKDIIENVEYEIDEDEEK"
     gene            1824..3224
                     /locus_tag="PLB33_00660"
     CDS             1824..3224
                     /locus_tag="PLB33_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62060.1"
                     /translation="MNNNYKIIRTIYENAFQRILECKDDDTGAIFYSNIITSKKVINL
                     ININDLKKIKSNILECYNTEDRIYIFTKSLETDCRKLTDLANSNLTMKQQFSLAEKSI
                     NLASNIYNMTDVVQQKILDIDRLFIDDNNDLIVDCNLIFEQEYDIADNETFKRLGNII
                     HMIFSGIEIIDYNISDSIPPDILKIIVRCLTREYVFPNDALVELKRSPIFNMLFGEEI
                     INNTVDIEQPEEPSLVIHNEESDDYLNIYLEDNQPAKKKALINKDIKRAAVSLLTVVM
                     VLLLGNFIIKKFNKNDEAEAINNVKPPLQEAPSETPVEEEPVYESTDIHFSEELLESI
                     GYEGAKAQKDTDIYVEGSSSLTVANSGQDKVKALFAAVDFKDDNFNYMLMKQIGVSGK
                     IKSQYDTQARVVFEAYKDGVIKSNFHSLVNIYDDIWSQFTVPINVTEADVLYIYLEYE
                     GENKVWIDSLFIDVIK"
     gene            3351..4049
                     /locus_tag="PLB33_00665"
     CDS             3351..4049
                     /locus_tag="PLB33_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62061.1"
                     /translation="MDIIRMQGVSKIYKSGQIQVEALKNISFNIKEKEFVSIMGPSGS
                     GKSTLLNLVGCLDHPTSGTYELAGNQVDKLSDSKMSDVRNQFIGFVFQSFHLLPRMTA
                     IKNVEIPLIYRGMSDKKRRELAENALELVGLKDRMTHLPAQLSGGQQQRVAIARAIVG
                     NPSIILADEPTGALDSKSGELIMELFVELNEKNSMTVVQVTHEEFIAEYGSRIIRLVD
                     GQIVSDDVVEVKNV"
     gene            4042..5784
                     /locus_tag="PLB33_00670"
     CDS             4042..5784
                     /locus_tag="PLB33_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG62062.1"
                     /translation="MYKKIISIAIVLGLVFFGGYLTAKWLIPKDTEASAGPRYSTKAV
                     ERGDIKQGVNLSGQLSGNWGGSITAPRPEGITDSNGMSVSVTYTVEEVFVEPNQMIKK
                     GDNLVRLSAVNLGDILTELTDSIQKKQEDIQAKISNLEKILNKDVTDISQVNAYDGIV
                     FRAPIRGRITELSVEEGEKVENFNIATIVDDSMVKISFKVNTYEFESLKEGQKVLLQY
                     RKTTEHGSQLAFDGFYPGTIKKLNKNKVPNGMTYVHTGTIEAENPGLVQPGMTVSIYT
                     ENNGIPVTALSYTGEVNSFVNEKKVTYSYLHGGENSNVIATEVYVSTNEFVEEGDLLV
                     RIAGSDVTQLIQNDIDEITNAYKEIENIYRRIDKISELTSKLMVTAPTDGMVAYVNYR
                     VGDQITANEQSDQWSLMLMDVYNTDEMYIYTNASDLDVLYIAQDAPVIVTVDALPGET
                     FDGKVMRLDSYTDYRTGETVYNVQIRVEGREGLRPGMNTNCFVDSGESLDTLLIPIEA
                     VFEENGKQKVEILKEDNQVEVVEIETGLMNDMYVEVLSGLEEGQLVVTGSTKDLMPSQ
                     SVPESDIILPGTNK"
     gene            5797..7146
                     /locus_tag="PLB33_00675"
     CDS             5797..7146
                     /locus_tag="PLB33_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62063.1"
                     /translation="MQARGNISNLFVRLKFSAMMALDGITSNLLRTFLTVLGISIGVA
                     AVISLMGIGEGARRSIVAQFESLGENVIVIKSNADNVKFEIEEAEILPERVTGIEYAT
                     PVIYTEDTPVRWRRDESEMNIVGVNESYPQIKDHPVISGNFFSSLHVKQRSPVVVLGY
                     NVAKSLLNGRSPVGYTMKIDGINYRIIGVLGEKGEGKGDGIDDKIVIPYTSAMKIAKT
                     KDILEIWAKSNSAEDAELAMVQLGRIYKRKVGLGSNIKPSTEEGEQPPGEGGGMDMPI
                     EEPMPEPVPEEPEEPGEKENVFEKGEEPLTITSLNNLVKEADQANRIMTVLLGGIAAV
                     SLLVGGIGIMNIMLVAVTERTSEIGVRRALGAKKEDLLIQFLLEAVYVSIIGCVAGIV
                     LGAWAISIVAKYGLSAVVSLDSIRIAVFVALTSGLLFGVYPAINASNLPPVEALKRQ"
     gene            7291..7674
                     /locus_tag="PLB33_00680"
     CDS             7291..7674
                     /locus_tag="PLB33_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOG62064.1"
                     /translation="MELNLSVGLKYKEEKTVTINDTAKVFGSGAAEVFATPMMIGLME
                     AASMNAVKNFLPRGCSTVGTLVNVKHMSATRVGKKVWAEAELIEIDRRRLVFNVTAYD
                     EDKKIGEGTHERFVIDDEKFMSNLK"
     gene            7704..8165
                     /gene="nrdR"
                     /locus_tag="PLB33_00685"
     CDS             7704..8165
                     /gene="nrdR"
                     /locus_tag="PLB33_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOG62065.1"
                     /translation="MRCPKCNYTESKVIDTRPTDDGFKIRRRRECIECGHRFTTYEKI
                     EETQIVVIKRDGTRQGYNRDKIINGLIRACDKRSVSLEQIEKIADKVEKQLYNTFQNE
                     VSTEIIGELVMNELQNVDDVAYVRFASVYRKFKDIDTFMDELTKILNERKK"
     gene            8175..8807
                     /locus_tag="PLB33_00690"
     CDS             8175..8807
                     /locus_tag="PLB33_00690"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HOG62066.1"
                     /translation="MYINLDVLNITNGNTHIIDSVFGRMNIEKIEAGKLKLLTGRIIT
                     TDPVLLYDDECFSEHVKPGLYTVNIYVGKCGKRKKQTVAAELRINENVPVKWEMALLE
                     GESAKGFSSDEFMGYEVENGLGCFMDEKVMEMLDIMSEEELNDYETKVKDAVRQSDSS
                     CADIIMDEEKGLNIIVFASGWNEGTFPTYYGFDKNNKLSRLVTDFMVIEK"
     gene            8971..9360
                     /locus_tag="PLB33_00695"
     CDS             8971..9360
                     /locus_tag="PLB33_00695"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOG62067.1"
                     /translation="MNKKINKYDIGLIVSIIIISLFFILYGGRDAVISNSKTAYIYSN
                     NKLVGEYVLTDDYKDVVNIESETGYNIMHIENGQIWIHEASCPDKICIYQGKISKNGE
                     MIVCIPNRMFIKIVDENDESEIDFIAD"
     gene            9373..9894
                     /locus_tag="PLB33_00700"
     CDS             9373..9894
                     /locus_tag="PLB33_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOG62068.1"
                     /translation="MNKIHRYIFLSLLTAAALILSIIEGMMPLPYIAPGAKLGLSNIV
                     ILSTVVVFGFKDALLVVIIRSILLMLVATNPITFIYSIASGIVSTIVMSLACKYLSNI
                     FSLIGISVLGAMAHNTTQVTVAAILFTTVNLYYYLPILSLVSIFTGCFVGYTAIFVTN
                     NLETTVKTWRRGV"
     gene            9894..10136
                     /locus_tag="PLB33_00705"
     CDS             9894..10136
                     /locus_tag="PLB33_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOG62069.1"
                     /translation="MRKGNSFYLILLILTSVIIGTILGKAFSNMLPILNYGEGIGFGP
                     ATLDLNIFTITFGFSARLTVAGIIGLIAGIIIFRKL"
     gene            10145..10720
                     /locus_tag="PLB33_00710"
     CDS             10145..10720
                     /locus_tag="PLB33_00710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOG62070.1"
                     /translation="MKKIVLASNSPRRIEMMKKYVDIITYIPDIVEKVNSFDYPQTTV
                     MKVAFEKALNVYLKYKENALIIAADTIVYLDEIMGKPKDEAEAYKMLSLLSGKTHYVF
                     TGICIIDTESSKKIVDYEKTQVVFNELTEGLIKRYLKTYEYLDKAGAYGIQGYGELLV
                     KEIHGCYNNVKGLPIAKLNNLLSKHFSYDLL"
     gene            10747..11436
                     /gene="radC"
                     /locus_tag="PLB33_00715"
     CDS             10747..11436
                     /gene="radC"
                     /locus_tag="PLB33_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOG62071.1"
                     /translation="MDNTNYTIKSLPADDRPQEKLLKYGAGALSNSELIAVILRTGTR
                     EENVVMLSQRILKEGGKGLRNIAEGSLEKFKSYKGISDAKAAKLMALAEISKRMSTLK
                     IEKLKISSPDDAAVMMMEEMRYYQKEYFKIMLLDTKNNVKKVSEISVGSLNSSIVHPR
                     EVFYEAVVNLASSIILVHNHPSGDCEPSHEDIVLTNRLDESGKILGIRVLDHIIIGDG
                     VYFSFKEEGLI"
     gene            11454..12605
                     /locus_tag="PLB33_00720"
     CDS             11454..12605
                     /locus_tag="PLB33_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG62072.1"
                     /translation="MGLFDFFTQKIGIDLGTSNTLVYVNNKGITLNEPSVVAINTTSY
                     EVCAVGFDAKAMLERTPKTIRAIRPLQNGVIADFEITRAMIKYFITKALNKKRFIKSN
                     IVISVPVGVTSIERRAVEEVAYDAGARKVKLVEEPMAAAIGAGLNVDQPIGNMIVDIG
                     GGTTEIAVISLGGIVESDSLRIAGDDIDINIKEYIKNKYKMDIGIVTAENIKKVLGNA
                     SKEYYKRIIDSDERIDSKEANEDNEDFEINEDNKDKKDLKDKKDKKEKKDEKENKKTD
                     YVQQMEIRGRDIVSGLPLKITITSDEVREAILETVNSIVNAIKRVLERTPPELSSDIY
                     TNGIYLSGGGALLKGLDIYLEKETGIKVFIADNPLQSVALGAGKISEEL"
     gene            12654..13487
                     /gene="mreC"
                     /locus_tag="PLB33_00725"
     CDS             12654..13487
                     /gene="mreC"
                     /locus_tag="PLB33_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOG62073.1"
                     /translation="MNFLKEHFKKILFFLTIFILIIMIGLSSIGRFEGADLGFIGNLI
                     AGFQKLTYKTGNTLTNSFYSVQDIVKMREENIMLTESIYKLREQVRILENIINKSEAL
                     ETEYEMRKNLTYDYVVGQVIAIDDSNWFSRFTIDKGENDGIRQNDIVIQAVKTDDGIV
                     QIGLLGIVTQTSSDWAKVITLLDEGCKVSFKNIENDESGIVQGSIDGNVTGYFFNSKA
                     GAEKGDNLVTSGIGEIYIPDIYIGDVKDVVKTTDASTLRIIVEPAVDFTKISRVYVLT
                     K"
     gene            13507..14001
                     /gene="mreD"
                     /locus_tag="PLB33_00730"
     CDS             13507..14001
                     /gene="mreD"
                     /locus_tag="PLB33_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOG62074.1"
                     /translation="MKKLILMSSLIVIGFVFQTSFIGFFNYFKILPNIPLILLVIFAM
                     LSDGVEGGVLGILTGILHDAMIYDVFGIYTLIYFIIGAVIGAFSDEMLRENKLVYSAV
                     TGISTVVMHLLLFLILFFLRFRVHLAKSILPNILFETALNAVLVIFVLKFVIYIFDRF
                     NVKA"
     gene            14008..17340
                     /locus_tag="PLB33_00735"
     CDS             14008..17340
                     /locus_tag="PLB33_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG62075.1"
                     /translation="MIKFFKNRFNILYLIIVLLLIILGFRMAVLTIVEGQEYRQIADI
                     KKIKDIPVKAPRGKIFDRNGIILADNVSSFTVQMYTDEIKPEDFNEIAYKLSKILDEN
                     MERPIDDFPIELDTFDYINESVEIIDSDLNLEEEIEVNYYRTAEEKVIEKVKENIHEW
                     LNFSISIYGENFSPKQRVHAAIINDNVPINLEGDKFEYEDEMLIQTDSDLTEAENETT
                     EQKTPIQIWLSEHNLKENISAEEAVAQLLVNNNKYLSSLFSNSKIRRLSYEFIIQKGV
                     ENIKLNEFSFIQDEEYENIKRDLNALNEEITMDTSAKDDFVILTMKNSIDNLLQTVYQ
                     GNEKIMPGAILIERLKEIYPDLPVKFVENGELGVFEYTDTETRNKYFNQQHMEYSTTA
                     YDFIKEIAFKNYDVLYNLVTDDNIKYYSQIELLKYVNPSISINEWEYTPLRNKKNWIN
                     NNVELDEETDDYSAKKVFEMLRESLKIPEEINDYDMRNMMVIRERYNIKSYLSYHPVD
                     ICYSISQKTVAMISERHHELNGINIEIEPLRYYPQYESASHIIGYLGKIAQDYELHEY
                     VVEKGYSTDDIIGKTGLEEKFEDYLRGEKGKKTVEVNNVGRTIRSVSSEAPVPGDDLY
                     LTLDLRLQKKAEESLKKGLEKIREGGVFESEWGDFQFRDKYENAYSGALVALDVKTNE
                     ILALANYPAYDLNLFSTGITTENWNSLLNDSKNPLAPRPLYNIAMLTAIQPGSTFKMI
                     TSLAALEKGVNPNTTVYCAGTMKVGDRDFRCWIYNMFGGRHGSQTMYQAIMNSCNFYF
                     YTTVLGENLATHQKHTVKVDAEDIIEMASKFGLDSKTGIEIDIPQEASGGVPSIEGKK
                     SGIRVYLRLFLEANIERYLNDGVVIDASMKNEIIEEIASWIDRDELMTRAEVYEGLLA
                     LNLNPDKTNDNYVPLVDIIKYSYLNQATWTVGDNLNISIGQGNNAYTALQMANYVSSI
                     ASGLRRNVSVVKEIKTYDGKKTDYIPLRESEKIELSDDSYLDIVKHGMKLVSYDDSAK
                     PFAIFPVEVGSKTGTAQNQGINPETGRPYDDFAWYVAFAPYDDPQIAVACVLFEGGSG
                     RYPIPIVREVIGEYLKINGLIE"
     gene            17376..18059
                     /locus_tag="PLB33_00740"
     CDS             17376..18059
                     /locus_tag="PLB33_00740"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG62076.1"
                     /translation="MNVILTGSQKNNIGKTIISIKLAIELANSGKNVLMMDLSSGKIK
                     MSEYLNVNEDIIYDIVDVVNKTCTLEQGIIEINENLSLLPCPRIAGKINEIKKESFIN
                     LLQSVEVYDYVIIDADKLTSVFIDFSIINNAITINNNDFSCIKEINTDKTISSNAGNF
                     IVAINKYNKKKAKKGIMMKSNDIEKLTETTISALIEENIKYSDLNHEKFLDDNFLKTE
                     IKNIVKNLK"
     gene            18202..18825
                     /locus_tag="PLB33_00745"
     CDS             18202..18825
                     /locus_tag="PLB33_00745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG62077.1"
                     /translation="MNKLTKKRLNIKRKEQVNKKLVLQVIFSIVLVAAVIVTKNINSN
                     LSEKIIEAADEKMSASIELLEVRDTIAKGFTTAINKLPFVGKEEASPVIGTLYRKYGL
                     NKTDDVSYYNHGIDIKSETQSVKALTDGKVMSVGKNEKHSGYIVIQDDDRTLIYGKIN
                     ETFVKEGDNISKGDVIGALDEENMILHIEVWEDGASINPSSLFDLTK"
     gene            18839..19357
                     /locus_tag="PLB33_00750"
     CDS             18839..19357
                     /locus_tag="PLB33_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG62078.1"
                     /translation="MLLILALLAHEVSHVFSAILLNIEFSKVKITLFGINLNAHLDNV
                     SHAKKIILFFAGPLCNLCFYFGFRNTEYFCFANMNLLLAYINLFPIVPLDGGNICKTI
                     LEIFLDTRTVSGYITMTNAFFILFFIIIIYIFKDYLFFLLVVMGLKGIVEENSYLLEK
                     SIIAVYNKINTK"
     gene            19402..19926
                     /locus_tag="PLB33_00755"
     CDS             19402..19926
                     /locus_tag="PLB33_00755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOG62079.1"
                     /translation="MESENIIIRKSTFDDCKIFTEWEQQDYVKEFLTINKERTYEEVV
                     REFILREQDPTKEQYTIILKNTGPIGRVYLSNISREFDFIDITRIYIGEKEHLGKGYG
                     RECMELLLDYCFNILKMERVTLDHYTGNKASNLYLKLGFKYEGIMRHAAKKDGKYYDL
                     HLMSMLREEYKQSI"
     gene            20097..20942
                     /locus_tag="PLB33_00760"
     CDS             20097..20942
                     /locus_tag="PLB33_00760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG62080.1"
                     /translation="MNKKPIVAIMYDFDKTLSPKDMQEYGFISELGMKSEDFWEKSRL
                     NMVNHNMDQILAYMLTMLQEAEGRMLLTRNIFNEMGRNVKLFEGLETWFDRINLYGEK
                     LGLEIEHYIISSGLKEIIQGTEIAHNFKEIYAAEYCYNENNIPIWPAMAVNYTSKTQF
                     LFRINKGVLDVTEHKGLNESTPDNKKRIPFCNMIYIGDGLTDVPCMKLVKINGGHSIA
                     VYHDDRCIADSLISQGRVDFVVSADYRENSEMEKTVQTILNQIAALNKVLKLHTKHMK
                     NLITD"
     gene            21376..21458
                     /locus_tag="PLB33_00765"
     tRNA            21376..21458
                     /locus_tag="PLB33_00765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21409..21411,aa:Leu,seq:gag)
     gene            21622..22485
                     /locus_tag="PLB33_00770"
     CDS             21622..22485
                     /locus_tag="PLB33_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOG62081.1"
                     /translation="MEVAILKSFTYENSGGNGAGVVVLNQQIPYDKKQEIANKVGLSE
                     TAFIYKRDENDYDVSFFTPVCEVDLCGHATIAAFYYLGKIGQIKGNDETKCVYQNTKA
                     GRLKIEITFKGQDVEYVLMQQSEPRIYGELKEKKQIADSLNIDERLIGLPNYDIAPTI
                     VSTGLKDILVPVSSRKVLNSLDVDFKMIEDISYENDVVGYHVFTIDNERVYARNFAPR
                     VGINEECATGTSNGALGSLLFSKGFSNQMEVIQGESMNQKSLIFVRIDSHNDIMDVYV
                     GGKAHIENYIK"
     gene            22497..22850
                     /locus_tag="PLB33_00775"
     CDS             22497..22850
                     /locus_tag="PLB33_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG62082.1"
                     /translation="MQIKEIVDIVKADVLTNYHDGNTEFKFGFASDLMSDVLAYGRSD
                     SLLITGLNNPQVIRTVEMLDINAVLFVRGKRPCEEVIELAKENGITILATDYTMFKTC
                     GLLVMNGMEGLEIND"
     gene            22843..23268
                     /locus_tag="PLB33_00780"
     CDS             22843..23268
                     /locus_tag="PLB33_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62083.1"
                     /translation="MTDKFLKRYSYNITANDFMSAGKSASAIKSNLKSLGIDSNTIRK
                     ISIISYETEINIVIHSYGGKLHCDIYENKIVIISEDTGPGITDLDMAMTEGYSTATDS
                     VRELGFGAGMGLPNIKKYSDQLEINTSSTGTTIIATIYL"
     gene            23278..24519
                     /locus_tag="PLB33_00785"
     CDS             23278..24519
                     /locus_tag="PLB33_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOG62084.1"
                     /translation="MNFSIMFDKDKCRGCTNCMKRCPTQAIRLIEGKAFINTAKCIHC
                     GECIKICPYNAYSPESIEWYNQMHDTPKKFKIAIPTTSLYGQFPRGTDICKVQNAILK
                     LGFDFVYDASWAADLVAKAIKLKIDKEKTMRPYISTYCPAAVRLIKYRYPSLMDNLIL
                     IREPMEIAGILARERVRKKFGLKDEDIKVFYISPCPAKLLAVTNPIGDYKPSVDWVIP
                     LNKIYGDLYREVIREDNITCSYPSLLGMKWAVAGGQSEHAELNNYIAVHGMENIIEIL
                     EEIENGKLSDIDFVEASACVNGCVGGTFNVVNPFIASSSINYISNSLPDECLNPDIDR
                     FDEMYKTGFFNYKLKEEEKFDKLNLKEAVERNEKIREILDKLPGLDCGSCGAPTCLAH
                     AEDVYNNESEFYDCVVLRAKK"
     gene            24532..24864
                     /locus_tag="PLB33_00790"
     CDS             24532..24864
                     /locus_tag="PLB33_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOG62085.1"
                     /translation="MNCLEFAEKLQLTPLCEKEGDIEIEGVYIGDLLSIVMAKAKKNY
                     VWITIQTHINIVAIAELLELSCIIVVENMAVDNDTLDKAKELNIPIFKTSESAYQVAC
                     RLSDMGIK"
     gene            24861..25550
                     /locus_tag="PLB33_00795"
     CDS             24861..25550
                     /locus_tag="PLB33_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG62086.1"
                     /translation="MKFYYDLHIHSDLSPCSACDMTPNNIINMSLIKGLDIISVTDHN
                     SAGNLRAVMELGNKTGIKVVPGLEVTTKEEVHVLCYFKELDNAIEFGNIIYNSLPFIK
                     NNPEIFGEQNIYNSKDEITGTLEKLLINASQYTIEDIFSLVKKYNGVMIPAHINKMTN
                     GILGVLGFMPINLKIETVEVYSKMPINSKIAEKYKVIYNSDAHQLVDIAEAVNYFDLD
                     DIEEIYEYLFE"
     gene            25692..26243
                     /locus_tag="PLB33_00800"
     CDS             25692..26243
                     /locus_tag="PLB33_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62087.1"
                     /translation="MKEISMHILDIAMNSIKADASLIEINIEDSIKNNRLSITIADNG
                     RGMSEDVINRVSNPFYTTRTTRKVGLGIPMLKEACERCNGYLKIESKIGVGTKVCCYF
                     ERDNIDRAPLGNMGETIMTIINSGDNFELTYSHKTDRGEFIFDTREVKEMLEGININD
                     IGVLMWIKEYINENVNLVSAGKI"
     gene            26400..27731
                     /gene="rsxC"
                     /locus_tag="PLB33_00805"
     CDS             26400..27731
                     /gene="rsxC"
                     /locus_tag="PLB33_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOG62088.1"
                     /translation="MSTKLFTFKGGIHPPQSKSFTQNVPIEDAPAPDIVVIPLSQHIG
                     APCDPLVAVGDKVKVGQKIGESKAFVSAPVHSSVSGTVKKIENRQVPGGAKVNCVIIE
                     SDGKYEVHESVQPKGKLEDLSKEDILSIIKEAGMAGMGGATFPTHVKLSPPKDKTIDT
                     LLVNGSECEPYLTADHRIMLEKPDLVLLGTKAVMKALGVNKAYICIEDNKPDAIETLQ
                     QAVGNEEGIEVVSMIAKYPQGDEKRLINAVTGRVVPSGGLPMEVGCVVENVGTVATIG
                     NVIKTGMPCIQRVVTVTGSAVENPKNLYVRIGTLFKDCIEMCGGYKEEPGKIISGGPM
                     MGIAQYTDEVPVIKGTSGILVLNKKEANVPEHSNCILCGKCVEACPVYLMPYKIARFS
                     LLRNFDGADEYHATDCIECGACSYVCPAKRPLKESISVAKKEILTRRKKAK"
     gene            27744..28694
                     /locus_tag="PLB33_00810"
     CDS             27744..28694
                     /locus_tag="PLB33_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG62089.1"
                     /translation="MEIKLIGSSSPHIRSDETTQKLMLDVIIALIPALAASIYYFGYK
                     AMILVLASVISAVATEYFCQKFMNRPITVTDLSAVVTGILLAFNLPAEAPWWIAVIGS
                     VFAIGIVKQIFGGIGFNFLNPALGARAFLMASWPHHLSGGFIDPAIDAVTSATPLSII
                     KGAASGQLPSLWDMLIGNIPGVIGETSAILLLAGGIYLIYTGTIKWIIPAFYIGTVAA
                     IALLFDAGNLSYHILGGGLMLGAFFMATDYATSPITDKGKVIYAVGAGMLTMFIRKMG
                     GYPEGVSYSILLMNILTPMIDKYVKPRVFGVATGGKKNEK"
     gene            28684..29256
                     /locus_tag="PLB33_00815"
     CDS             28684..29256
                     /locus_tag="PLB33_00815"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG62090.1"
                     /translation="MKNNFVKLAGVLLIIGAVSAGVLAWLNDVTSDRIAQNEAMASMD
                     PAILEAVMPGSVMFNDYEDTALVDTIKADNNKFINLLTAVDESGNELGKVIRTWSTVK
                     GFNGDLELYVGFTPDGQISGTSVISHAETEGVGSKVTLLAFTEQFIGKDASGEISEGD
                     YDAVSGATYSSKSFLSAVNNAINVYAEYVK"
     gene            29266..29862
                     /locus_tag="PLB33_00820"
     CDS             29266..29862
                     /locus_tag="PLB33_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOG62091.1"
                     /translation="MNKLEIFKNGVTTENPILVQQVGMCATLAITTSLINGIGMGVAV
                     IAVLTGSNIVISLLRKFIPDEVRIPSFIIVIAAFTTIIDLLMHAYTFELYKALGVFIP
                     LIVVNCIILARAEAFASKNSLSDSIIDGLGMGLGYLVAMILLSSVREILGNGTILGFR
                     ILPEAYEPMMMAIQPPGAFILLGLLIGIVNYLSRRKKA"
     gene            29873..30448
                     /gene="rsxA"
                     /locus_tag="PLB33_00825"
     CDS             29873..30448
                     /gene="rsxA"
                     /locus_tag="PLB33_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOG62092.1"
                     /translation="MELVSLFITSFIVNNIIFMQFLGTCPFLGVSKKIDTAAGMGIAV
                     IFVITLASVITYIIHYAILVNLELEYLQTIVFILIIAALVQFVEMFLKKSAPGLYQAL
                     GIFLPLITTNCAVLGVAINNIIYEYNFVESLVYSVGISAGFLLAIVLFAGVRDKVDYA
                     DLPESLKGSAISLITAGLMSVAFLGFSGLQL"
     gene            30460..31269
                     /locus_tag="PLB33_00830"
     CDS             30460..31269
                     /locus_tag="PLB33_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOG62093.1"
                     /translation="MVIVKAVAVTGGIGAICGLLLALAAKLFHIDVDQRITDVRNALP
                     GANCGACGYAGCDAMAEAIAAGRAPVDGCPVANYQAHAKIADIMGTSAEEGEKKVAHV
                     LCRGNDCLASKKHEYHGVRDCKAAAAVQGGDKSCDKGCLGYGNCVRVCDFDAIDIIDG
                     IAVIDKEKCTACGKCIEECPKAVIEFVPYKNNVIVGCNNRDFGKAVKDVCKIGCIACK
                     ICEKNCPYDAIHVTNNLAKVDYDKCTHCMICVEKCPTKVIEGDLSIKDKAV"
     gene            31411..32868
                     /locus_tag="PLB33_00835"
     CDS             31411..32868
                     /locus_tag="PLB33_00835"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOG62094.1"
                     /translation="MYYNNFGERRLSMLMDFYELTMANGYFSNGMKDEIVYFDLFFRK
                     NPDGAGFSIVAGLEQIIQYIKELKFYEEDINFLRSKKLFTEEFLQYLKDFKFSGDIYA
                     IPEGTPVFPNEPLITVRAKTIDAQIIETMVLLTINHQSLIATKANRVVRAAKGKPIME
                     FGARRSQGYDGAIYGARAAYIGGVIGTATTLADEAFGVPALGTMAHSWVQMFESEYEA
                     FKSYAQNYPDACNLLVDTYNVVKSGIPNAIRISKEVLEPMGKRLKGVRIDSGDIAYLS
                     KKCRKMLNEAGLHDCAIVASNSLDEFIISDLLDQGAKIDYFGVGERLITAKSEPVFGC
                     VYKLVAVERDGKIIPKIKISENEEKITNPGYKKVWRLYDKKTNSPIADVITLADEIID
                     NSKPYTIFDEIHTWKKKKVTSFYAKNLQVPVFINGECVYESPSLNEIREYCKKKVGNL
                     WGEIKRFSNPHHYYVDLSKELWDCKQHLISQYSFK"
     gene            complement(32970..34160)
                     /locus_tag="PLB33_00840"
     CDS             complement(32970..34160)
                     /locus_tag="PLB33_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOG62095.1"
                     /translation="MERIVGTVVRGLRAPIITKGDDIVKITVETVLKASKTEGFSLRD
                     KDIVGITEAIVARAQGNFANIEQIALDVKQKFDSDTVGLIFPILSRNRFAVCLKGIAK
                     SFKKIYLMFSYPSDEVGNPLVDYDLLDKEGVNPWTDILKENEFRSHFKNTKHIFTGVD
                     YIKYYSEIIRESGCEVEVIFANNPISILNYTKNVLTCDIHTRNRSKRLLKSNGAETVY
                     GLDDILNQSVEGSGFNEKYGLLGTNKATEDTVKLFPRDCQSLVEKIQEKLFELTGKKI
                     EVMIYGDGAFKDPVGKIWELADPVVSPGYTEGLIGTPNEFKLKYLADNQFPHLKGDEL
                     KKAICDYIMNKDCDLKDRMESQGTTPRRLTDLIGSLCDLTSGSGDKGTPIVYIQGYFD
                     NYSV"
     gene            34411..34935
                     /locus_tag="PLB33_00845"
     CDS             34411..34935
                     /locus_tag="PLB33_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOG62096.1"
                     /translation="MIKIGMRNIKTTISVFICLLVFELINRNNPIFACIAAVICVQNT
                     IVDSKEIGFSRIIGTIIGGLAGAFTLFIVNTFFNHKILIFIIPLGIMLLIQICVTINM
                     KKAVVICCVVFLVITLNHDEGYILYTFNRVLDTSIGIIIALFVNKYVKIPEKLKSKIG
                     DKEELIETHENSEP"
     gene            35036..35305
                     /locus_tag="PLB33_00850"
     CDS             35036..35305
                     /locus_tag="PLB33_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOG62097.1"
                     /translation="MTGADLGIILAYTAGIMLVFMTSWIFFKPLKFIGKIILNSVIGA
                     IVLIIFNYFGQYTGVHIGVNEFTALIIGILGVPGFFAILLVKLLI"
     gene            35566..37011
                     /locus_tag="PLB33_00855"
     CDS             35566..37011
                     /locus_tag="PLB33_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG62098.1"
                     /translation="MKKLNIISGIKQYRSEVDTNFHMPGHKGKPEILGELEKNLNFFD
                     ITETLGTDNLHYPTGFLKDAMNYISKVYGTKKSYMVVNGTSCGIISAIMACTNPGDKI
                     LVQRDCHKSVYNACILGDLKLFYLYPDFNKKYGLNFSISLDKLDQMLTDNPDIKMVVL
                     TYPTFYGICFDVKKAAEIVHKHGKILMIDEAHGSHLQFCKELLPPPAENSGADIVAQS
                     THKTLPCLTQGSILHICSDRVNVNNIETMLRMLQTTSPSYILMASIENAIHWMAENGE
                     ERLKRNIEVFKRRTLELRNMGINVLEDDFLISEGLYDFDATRAVISMSELGITGTELQ
                     DILRYKYKIQMEFADLQYTVGYVTATDEPEDIDRLFDAVKEIYIEESKSKEKREIVQI
                     EPFPKLEHERKMRKAFYAENMLLHMDDAIGKTAAEFIIPYPPGIPLVCPGEIIVQDTI
                     DYVKIMLENGINVNGVNKNNQVRVVKWKEDL"
     gene            36993..37601
                     /gene="tmk"
                     /locus_tag="PLB33_00860"
     CDS             36993..37601
                     /gene="tmk"
                     /locus_tag="PLB33_00860"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOG62099.1"
                     /translation="MEGRFIVLEGSDGSGKSTIAERIGRYYSEKGRDIEFTREPGGTS
                     ISEKIRELILDRDNTEMDYRTEALLYAASRAQLVSEKIIPWLKAGKVVISERYVYSSL
                     VYQGLGRGLGIDEIKKINDFATAGIKPDLVMLLDVEPEKALKRKLCIDGGDRLENEDI
                     SFHRSVYEGYKKLSSHFPEIITIDAERSIEEIFEEIKGLLTI"
     gene            37612..37944
                     /locus_tag="PLB33_00865"
     CDS             37612..37944
                     /locus_tag="PLB33_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOG62100.1"
                     /translation="MKLITAVVQNDDANKLISALRENGISSTKMSSTGGFLSSGNTTV
                     ISGIEDDRVDEAIEIIRKICKTKKKVTAAIPPSSFSTVGGYLPQTIEVTVGGAVVFVT
                     PVDRFEKI"
     gene            37955..38926
                     /gene="holB"
                     /locus_tag="PLB33_00870"
     CDS             37955..38926
                     /gene="holB"
                     /locus_tag="PLB33_00870"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOG62101.1"
                     /translation="MLYSQVAGQEEIKTSLIKSIDNNQVSHCYLFEGPEGMGKFELAL
                     VFAQSLLCLNFHKEPCNKCKNCIKVNSLNHPDLHIINKDGNSIKREDIDLLINTVYNK
                     PYEAKRKIFIIKDAHLMTPQAANGFLKTLEEPPGDTVIILLTINSNLLLPTIVSRCQE
                     IKFKNISKDSIKSYLKDYDSDRVELAANYSKGILSKAINIIKGSDDTLKKREEIIKIF
                     DKIVNSDSEIIFELESYFDDKKDDINSIIEIMIMWIRDIIFVNNNMDRLVINKDYINL
                     LKTHGKNMKKDTDIIEYMQNTSDNIKSNVNYKLTVDNMLLKIQEVFK"
     gene            38923..39813
                     /locus_tag="PLB33_00875"
     CDS             38923..39813
                     /locus_tag="PLB33_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOG62102.1"
                     /translation="MIKVVGVRFKKAGKIYYFDPEKKWINNGDFVIVETIRGIEFGQV
                     VVGPKMVKEEDIVQPLKKVLRLATEDDIKRNRENKEKEKEAYDICFKKIEEHGLEMKL
                     IDIEYTFDNNKVIFYFTADGRIDFRELVKDLAAIFRTRIELRQIGVRDEAKMIGGLGP
                     CGRHMCCSSFLGEFYPVSIKMAKEQKLSLNPAKISGICSRLMCCLNYEHQVYEENIKK
                     LPDIGDKVQVLGTNKTGIVIDINPLFVTAKVNVEKSDGTIEVEEIQGSRLKVVEEGVL
                     KHYDAEDTDIDIEELKELED"
     gene            39925..40095
                     /locus_tag="PLB33_00880"
     CDS             39925..40095
                     /locus_tag="PLB33_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG62103.1"
                     /translation="MAYIITDACISCGACEPECPVGAISAGDDKYVIDAATCIECGAC
                     ASVCPVEAPQQD"
     gene            complement(40170..41864)
                     /locus_tag="PLB33_00885"
     CDS             complement(40170..41864)
                     /locus_tag="PLB33_00885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOG62104.1"
                     /translation="MKFNEYEYIRPDYNIIKEKYLNLINDFKNSATADEQYTILKKIN
                     ALANNVDTMSQLVYIRNSINSADEFYDTEKDYMDMTSPKFYGLRIEFYKQLLNSRFKE
                     ELTDKIHPQLITLAEMEIKTFSDEAIPLMQEENKLISEYDKLISSAKIEYDGKLLNLS
                     QMQPYMESKDRRIRKEAHEKSSNFFKENEAKFDLIYDKLVKIRHQIALKLGFNNFIEL
                     AYLRNGRSDYNSTDVRNFREHVLKYIVPITNELRERQAKRIGVDEFKYYDIPLNFISG
                     NPTPKGNKDWIMERTKKMYEELSPETHEFINIMIDRELFDLETKPNKQAGGYCTFLHD
                     YKSPFIFANFNGTQDDVTVVTHEAGHAFQTYQSRHFDAPEYSFPTSEAAEIHSMSMEF
                     ITWPWMELFFEKDIEKFKFFHLADSLCFIPYGVTVDEFQHIVYEYPDMTPDERKKAWR
                     DIEKKYTPYKNYDDNEFLNKGTFWFKQGHIFSSPFYYIDYTLAQICSYQFWLRFNENR
                     KEAWEDYLKICKIGGSQSFLEILKAGNLKSPFEESTINSVASKIKEYLNTINDMKL"
     gene            42078..43373
                     /locus_tag="PLB33_00890"
     CDS             42078..43373
                     /locus_tag="PLB33_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOG62105.1"
                     /translation="MKNIIVKVLKNSIAEELNIEPGDELLSVNNSIIKDYIDYKYQIS
                     DNLVYFEIKKKDGEIWELEIEKDYDEDIGIVFENPLMDNIKVCSNKCIFCFIDQMPKG
                     MRKTLYVKDDDTRLSFLYGNFITLTNLSDDEFDRIIQYKISPIKVSVHTTDPQLRKYM
                     MGNDKDMDILKYLKRLIDANITVDCQIVLVRDVNDKEQLTKTINDLSSLHPGLRSVAV
                     VPVGLTKYREKLTELKPFDKESAKDVVEQVTKLQETLLRKFGTRFIFIADEFYILSKY
                     DFPPYEDYEDFDQLENGIGMCRLFKNRVEEEIKNIRYNKVLKDEVTFVTGVAAYELMV
                     ELAEKIMSRINVKINVLRIINNYFGDKITVSGLITGKDIIDQLKGKNYKNLILPRNMI
                     NDNHVMLDDMVIEDLERELNTKVEIYDAEDDTLLSLIVK"
     gene            43387..44706
                     /gene="der"
                     /locus_tag="PLB33_00895"
     CDS             43387..44706
                     /gene="der"
                     /locus_tag="PLB33_00895"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOG62106.1"
                     /translation="MGRPIVTIVGRPNVGKSTLFNRIAGKRISITEDTPGVTRDRIYA
                     ECEWLDNYFTLIDTGGIEPNTTDTIFAQMRIQAEVAIEMADVVLFMVDGKEGVTAADT
                     EVAQMLRKAKKEIILVVNKMDRFVNEDSVYEFYNLGLGDPIPISSAEALGIGDLLDEI
                     ISKFNGKMDTKEEEDSIKIAVIGKPNAGKSSIINRIIGEERVIVSDIPGTTRDAIDTK
                     FERNSKKYTLIDTAGIRKKSKVLESVEKYSVLRAFTAVERADICVLVIDADKGVTEQD
                     IRIAGYSHDSNKGMVIVINKWDLIEKDNSTFKEFTEEIRQKLAYATYAPILTVSALTG
                     QRVNKILEVVDEIYSFRNLRISTGVLNDILTEAVLMNQPATVKGKRLKIYYGTQVAVN
                     PPKFLIFVNDKEIVHFSYERYIENKIREAFEYKGTPIIIEFRNRSEK"
     gene            44751..45332
                     /gene="plsY"
                     /locus_tag="PLB33_00900"
     CDS             44751..45332
                     /gene="plsY"
                     /locus_tag="PLB33_00900"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOG62107.1"
                     /translation="MKYIFIAIISYFIGNISFAYILGKILTNKDVREFGSGNAGTTNA
                     IRAFGKKVGAMVFSGDVLKGVVAVLFGRYFAGQTGAYIAGAFAIIGHNWPVLLNFKGG
                     KGVATTIGVMVFVNFYLTLICVIIGVIIIIFTRTVSLGSISGMAVAPLVAIILIRPLT
                     FDLLLFTIFVASMSIYRHKDNIKRLIKGNENKF"
     gene            45342..46340
                     /locus_tag="PLB33_00905"
     CDS             45342..46340
                     /locus_tag="PLB33_00905"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOG62108.1"
                     /translation="MNKKITVLGGGSWGTALSKLLSENGHNVTVWLRDEAQAKELTVK
                     RVNEKYLPKVKIPENIVFASDINKAVEEAEILLLVIPTQMVRGVLKQIKDEYKDGKII
                     INASKGIEKGTMKLVSTIVKEETKNTIFAVLSGPSHAEEVGLSLPTAITVACENKDVA
                     EEIQDIFMSEYFRVYTNEDVLGAELGGALKNIIALGAGISDGVGYGDNAKAALMNRGI
                     VEIARLGLAMGADLHTFYGLTGIGDLIVTCTSKHSRNWNAGYLIGQGLTKDEAIKKVG
                     MIVEGIPTTYAAYELSKKLMVEMPIVDAMYDVLENKADVKDTVNKLMLRDKKEERL"
     gene            46533..47996
                     /gene="spoIVA"
                     /locus_tag="PLB33_00910"
     CDS             46533..47996
                     /gene="spoIVA"
                     /locus_tag="PLB33_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOG62109.1"
                     /translation="MYEDIAKRTKGEIYLGVVGPVRTGKSTFIRKFSENVMLPNIEDE
                     YTRERANDEMPQSGTGKTVMTTEPKFVPSDAVMVTFSNNVRAKLRLIDCVGYMIPGAI
                     GNMEGEVPRLVSTPWNDEPIPFAEAAEIGTKKVINDHSTIGVLVTTDGSITDINRDNY
                     IMAEERVVRELKEINKPFVIILNTTHPKNENTVALSYQLKEKYDAPVLIVDCMNLKTD
                     DIENVLENVLYEFPIKEINVELPRWFDGLSYDYPIKRNLINILKDDFENYYKLSDFKA
                     EDIFSEENDYVEDYYVKDIELGTGKINLAVDLDNSYYYKIISDLCGEEITGEHQILKL
                     IGDLSKTRKSYSRVEAAINDAKANGYGYVTPTVSDMIIEPPEVFKEGGRYGIKIKAQA
                     PSLHIFSAGVNTEVSPVIGSKTQSEDLMCYLTEQMKENPQSIWQAEMFGKPLSSLVEE
                     QLDSKLTAMPDNARRKLRKTIEKIVNDGQGSIIFLIV"
BASE COUNT        17488 a         6784 c         9850 g        14343 t
ORIGIN      
        1 cttcgatatg ttaaaactta caaaaagaat agaaaatgat agaatactac taaatttgac
       61 ataaaattaa tatttttctt gacaaaataa gtttttttat gtaagattat taaaagttta
      121 attttttgta acaatcaaat ttaaggagcg taataaatga aagtcttgat tgttgatgat
      181 gaacttttaa ccattaagaa tcttaaatat agtttcgagc aggataatta tgaagtggaa
      241 tctaccggtg acagcaatga agctttaaaa aaaattacaa cgactgatta cgatattata
      301 atacttgatt tagtgcttcc tcaaacaaat ggtttagaag tttgtcaaaa aattagagaa
      361 atatcgaatg ttccgatttt gattttgtca gcaaacaatg atgatatgag caaaatctta
      421 ggattggaat atggtgctga tgattatatt accaagccct ttaatatttt agagcttaaa
      481 gcacgaatga aagcaatttt aagaaggaca agaggtacga gccaaaaagc aaatgaacag
      541 actttggtgg tggatgattt tataattaat gccttaggca ggaaagtaag tcttcataag
      601 caggaaataa atcttaccgc gaaagaattt gatttgttgc ttttattaat aacaaacccc
      661 gggaaagtat tttccaggga ggaactttta gaaataatat ggggttatga atactacggt
      721 gatttgagaa ccgttgatgt ccatgttaga agattgaggg aaaaaataga aacgaaaaac
      781 aaggcatatg agtatatact cacaaaatgg ggagtaggat attattttag aaacaggaat
      841 taaatgtcct cgtactgccc atactgctgc atattatgca gcttttttta attcagtcat
      901 tacgatagta aaatcagctt gaatttatca ggtggatatg gtaatatatt tgtgatgggg
      961 atagataggt gctggtgtgc ctcctggtct tcaaaatcag ttgcggggcg gaatcgtcct
     1021 gggtgggttc aattcccaca tattcccgcc aaattaagga gataaattat gcctaattgt
     1081 gttttggtat gtgtaacaag acagaaaaca agcgaaaaat tgataaaaaa aggagtggaa
     1141 ctgataaaag gagaagctgg cagctgttta aatgttatac atgttgtaaa tgaaaatgac
     1201 aagtttttgt atggtttaag cgacggagat gcgttagaat atttatttga aattactaaa
     1261 gatgtgggtg ctgctaatct agttgttaaa agatccaagg atgtaataaa gacaatagtg
     1321 gaatatgcgg aagaaataaa ttgcactcat attgtattgg gccactcgaa caatccatca
     1381 aacaatttta tcgctaagct gcagaggaag cttcctaatg ttgagttcat aatctaggat
     1441 gcgaatatgg gcagaatttt atatataata aaatttttat ttgataaaaa tattcccata
     1501 agggaaaagt ggtgggttat aatacctctt atttacatat tgagtcctgc ggatctgata
     1561 ccttttccaa tattggggct cggaatagta gacgatttgg taatgcttgg atttttatta
     1621 acaatagtct atgataaaac caagaaatat tattttgata aaagacatga agacaaagat
     1681 attatagaaa atgttgaata tgaaattgac gaggatgagg agaaataact catccttatt
     1741 tatttgtaaa ataatacaaa aatgtcttta tttttgtcga aatacttgtt aatatatagt
     1801 taataatatt tggaggtctg tatatgaata ataattacaa gattatccgt acaatttatg
     1861 aaaatgcttt tcaaagaatt ttagaatgca aggacgatga tacgggtgca attttctact
     1921 ccaatataat aacaagcaaa aaggttataa atttaataaa cataaacgat ttaaaaaaga
     1981 ttaaatccaa tatattagag tgctacaata cagaggatag aatttatata tttacaaaat
     2041 ctttagaaac agattgcaga aagttaacag atttagcaaa cagtaacctc accatgaaac
     2101 aacagttcag tcttgcagaa aagtcaataa atttagcctc aaatatttac aatatgaccg
     2161 atgtagtaca gcagaaaatt ttggatatag accgattgtt catcgatgat aataatgatt
     2221 tgattgttga ctgcaatctg atatttgagc aggaatatga tatagcagac aatgaaacat
     2281 ttaaaagatt gggcaatatt atacatatga ttttttccgg aatagaaatt attgattata
     2341 acatatccga ctcaatacct cctgacatat tgaaaataat agtaagatgc cttacaagag
     2401 aatatgtttt tccaaacgat gctttggtgg aattaaaaag gtcgcctatt tttaatatgc
     2461 tttttggaga agaaattatt aataatactg tggatattga acaacctgag gaaccgtctc
     2521 ttgtaataca caatgaagaa tcggacgatt atctcaatat ttatttggaa gataatcaac
     2581 cagctaaaaa gaaagcactt ataaataaag atatcaaaag agccgctgta tcattgctta
     2641 cagttgttat ggtacttctt ttgggtaatt ttataataaa aaaattcaat aaaaatgatg
     2701 aggccgaagc aattaacaac gttaaaccac ctttgcagga ggcaccttca gaaacaccgg
     2761 ttgaagaaga acctgtttac gagtcaacag atattcattt cagtgaagag ctgttggaga
     2821 gcatcggata tgaaggagca aaagcacaaa aagataccga tatatatgtg gaaggaagct
     2881 cttccttaac agtggccaat agtggacagg ataaagttaa agcactgttt gcagctgttg
     2941 atttcaaaga tgataatttc aactatatgc ttatgaaaca aataggggtt tcaggaaaaa
     3001 tcaaatcaca atatgatact caagctcggg tagtttttga agcatataaa gatggtgtta
     3061 taaagtcaaa ttttcattca ttggttaata tatatgatga tatatggtca cagtttacag
     3121 tacctattaa tgtaacggaa gctgatgtct tgtatattta tttggaatat gagggtgaaa
     3181 ataaagtgtg gattgattcc ttgtttattg acgtaatcaa ataattaatc aaactgacta
     3241 ttggaacgac ccggtcgttc ttttttttac aaaattatta atgtttactg aactttttct
     3301 taagtttata cgtctaatat atggactgat gtgtaaaaga gaggtggaag atggatataa
     3361 tcagaatgca aggtgtttca aaaatttata aatcaggtca aattcaagtg gaagctttaa
     3421 aaaacatttc cttcaatatt aaagaaaaag aatttgtttc aattatggga ccttccggct
     3481 cgggaaaatc aacactgtta aacttagtgg gatgcttaga ccatcctaca agcggaacat
     3541 atgaattagc aggaaatcag gttgacaaat taagcgattc taaaatgtca gatgtaagga
     3601 accaatttat cggatttgta tttcaaagct ttcatttgct gccaaggatg actgcaataa
     3661 aaaatgttga aattccttta atatataggg gaatgagcga taaaaaacga agagagctgg
     3721 ccgaaaatgc attggagctg gtaggactga aggacagaat gacacatttg cctgctcaat
     3781 tgtcgggcgg ccagcagcaa agggtagcta tagccagggc tatcgtagga aatccgtcaa
     3841 ttatattagc ggatgagcct acaggagcat tagattcaaa atcaggcgaa ttgattatgg
     3901 aattatttgt agaattaaat gaaaaaaact caatgaccgt cgttcaggta actcacgaag
     3961 aatttattgc agaatacggc tcaagaatca taagacttgt ggatggtcaa atagtatctg
     4021 atgatgtagt ggaggttaaa aatgtataaa aaaattatat cgatagcaat agtattagga
     4081 ttagtatttt tcggcggata tttaacggca aagtggctta ttcctaaaga tacggaagca
     4141 tctgcaggtc caagatattc aacaaaggca gttgaaagag gagatataaa acaaggagtt
     4201 aatttatcag ggcagcttag cggtaactgg ggaggctcaa taacagcacc aagacctgaa
     4261 ggtataactg attcaaacgg tatgagcgta tcggtaacat atactgttga agaagtgttt
     4321 gttgagccta accaaatgat taaaaaggga gataatttag ttcgtttatc tgccgtaaac
     4381 ttaggtgaca ttttgactga attgaccgac agcattcaaa agaaacagga agatattcag
     4441 gcaaaaatat ctaacttaga aaaaatatta aataaggatg ttacagatat aagtcaggtt
     4501 aatgcttatg atggaatagt atttagagca cctattcgag gacgaattac agaactttcg
     4561 gttgaagagg gtgaaaaggt agaaaacttc aatattgcta ctattgttga tgacagtatg
     4621 gtgaaaattt ctttcaaagt taatacatat gagtttgaaa gtttgaagga aggtcaaaaa
     4681 gtattgctgc agtatagaaa gacaacggaa catggctccc agcttgcttt tgacggtttc
     4741 taccccggga ctataaaaaa attgaacaag aataaggtgc ccaacggtat gacatatgta
     4801 catacaggta caattgaagc tgaaaacccc ggtcttgtac aacccggaat gactgtttct
     4861 atttataccg aaaacaatgg aattcctgta actgccttat catatacagg agaagtaaac
     4921 tcattcgtaa atgaaaagaa agtaacttat tcttatcttc atggcggtga aaattctaat
     4981 gtaattgcta ctgaagttta tgtaagcaca aatgaatttg ttgaagaagg agacctgctt
     5041 gtccgtatag ctggaagtga tgtaactcaa cttattcaaa atgatataga tgaaataacg
     5101 aatgcataca aagaaataga aaatatttac agaagaatag ataaaatatc cgagcttaca
     5161 tcaaagttaa tggttacggc tcccacagac ggaatggttg cttacgttaa ttacagagtc
     5221 ggtgaccaga taacagcaaa cgagcaaagc gaccagtggt ccttgatgct gatggatgtg
     5281 tataatactg atgaaatgta tatctacaca aatgcttcgg atttggatgt tctttacata
     5341 gctcaggatg cgcctgttat agtaacggtt gatgcacttc ccggagaaac tttcgatggc
     5401 aaagtaatga gattggattc ctatacggat tacagaaccg gggaaactgt atataacgtg
     5461 caaattagag tcgaggggcg agaaggatta aggcccggta tgaatacaaa ctgttttgta
     5521 gactccggag aatcattaga tacactttta ataccgattg aagctgtctt cgaagaaaac
     5581 ggaaaacaaa aagttgaaat tcttaaggaa gataaccagg tagaggtagt tgaaattgaa
     5641 acaggtttaa tgaatgatat gtatgttgag gttctaagcg gattagaaga agggcagctt
     5701 gtcgttaccg gcagcactaa agatttgatg cccagtcagt ctgtgcccga gagcgatata
     5761 atactgcccg gaacaaacaa ataaggggtg atgataatgc aagcaagagg gaatatttct
     5821 aacctgtttg taaggttgaa attttctgcc atgatggcat tagacggcat aacatcaaat
     5881 ttgcttagaa catttttaac agtccttggt atttccatcg gtgttgcagc tgtaatcagc
     5941 ttaatgggca tcggtgaagg tgcgagaaga tctatagtag cacaatttga aagcttgggt
     6001 gaaaatgtta ttgttattaa atcaaatgct gataatgtaa aatttgaaat agaagaagca
     6061 gaaatactgc cggaaagagt aaccgggatt gaatatgcaa ctccggtcat atatacggaa
     6121 gacacacctg taaggtggcg aagagatgaa agtgaaatga atattgtggg agtaaatgaa
     6181 agttatccac aaataaagga ccaccccgta atcagcggaa actttttttc atcccttcat
     6241 gtaaaacaga ggtctccggt tgtggttttg ggctataatg ttgctaaatc gcttttaaac
     6301 ggcagaagtc cggttggcta taccatgaaa attgacggta taaactacag aataatagga
     6361 gttctgggag aaaaaggaga aggtaaggga gacggaatcg atgataaaat tgtaataccc
     6421 tatacttctg ctatgaaaat agcaaaaaca aaggatatat tagaaatatg ggcaaaatcc
     6481 aacagtgccg aagatgcgga acttgcaatg gttcagcttg gaagaatata taaaaggaaa
     6541 gtcggtttag gaagcaatat taaaccgagt actgaggaag gcgagcagcc tccgggagaa
     6601 ggcggaggaa tggatatgcc aatagaggag ccaatgcctg aacctgttcc tgaagaaccg
     6661 gaagaaccgg gtgaaaaaga aaatgtcttt gaaaagggtg aagagccgct tacaattact
     6721 agtttaaata atttagtaaa ggaagctgac caggcaaaca gaataatgac agtactactt
     6781 ggaggtattg ctgctgtatc gcttctggta ggcggtatag gaattatgaa cataatgctt
     6841 gtggcagtga cggaaagaac aagcgagata ggagtaagaa gagctttagg agctaagaag
     6901 gaagatttat taattcagtt tttattggaa gctgtctatg taagcataat cggatgtgtt
     6961 gcaggaattg ttcttggtgc ttgggcaata agtattgtgg caaaatacgg actttctgca
     7021 gttgtaagtc ttgattcgat cagaatagca gtatttgttg cactgacttc cggacttttg
     7081 ttcggtgtat atcctgcaat aaatgcttca aatttaccgc cggttgaagc attgaaaaga
     7141 cagtaagaac gtttggtgac atgcttttct cggtgccagg caccatggcg gtgcctggca
     7201 ccaatgtcta tgaggtttta atttcaaaac ctcttttatt ttatattatt ttattgtata
     7261 atacggtaag aacaggtatt ggaggtaata atggaattaa atctatcagt tgggttaaaa
     7321 tacaaggaag aaaaaactgt tacaataaat gataccgcaa aagttttcgg cagcggtgct
     7381 gctgaagttt ttgcaacgcc tatgatgatt ggtctaatgg aagcagcttc catgaatgcc
     7441 gtaaaaaatt ttttgccaag gggctgttca actgttggaa ccttagttaa tgtaaaacac
     7501 atgagtgcta caagagtcgg caaaaaagtg tgggcagaag cagagcttat tgaaatagac
     7561 agaaggagat tggtttttaa tgtgacagct tatgatgaag acaaaaaaat aggagaagga
     7621 acccacgaaa gatttgttat tgatgatgaa aaattcatga gtaatttgaa ataatttgtc
     7681 attgctatga aaaggagtaa tttatgagat gtccaaaatg taattataca gagagcaagg
     7741 ttattgatac aagacctacc gatgatgggt ttaaaataag acgtcgaagg gaatgcatag
     7801 aatgcggaca tcgttttact acatacgaaa aaatagaaga aactcaaatt gtagtaatta
     7861 aaagagatgg aacaaggcag ggttataaca gagataaaat aataaacggt cttatccgtg
     7921 cttgcgataa acgctccgta agtcttgagc aaattgaaaa aattgcggat aaggttgaaa
     7981 aacaattgta caatacattt caaaatgaag tttcaaccga aattatcggg gaattggtca
     8041 tgaatgaact tcaaaatgtg gatgatgtgg catatgtaag atttgcatca gtttacagaa
     8101 aatttaaaga cattgatact ttcatggatg aattaacaaa aattttaaat gaaagaaaaa
     8161 agtaaggtga taagatgtat ataaacttag atgttcttaa tattacaaac ggcaatacac
     8221 atatcataga ctccgtattt ggcaggatga atattgaaaa aattgaagca gggaaactta
     8281 aattgttgac cggcagaata ataaccacag acccggtatt attgtatgat gatgaatgtt
     8341 tttcggagca tgttaagccg ggattatata ctgtcaatat atatgttgga aaatgcggaa
     8401 aaagaaaaaa acaaactgtt gccgccgagc tgagaatcaa tgaaaatgtg cctgttaaat
     8461 gggaaatggc tttattggaa ggtgaatccg caaaagggtt cagcagcgac gaatttatgg
     8521 gatatgaagt tgaaaacggg ctcggatgtt tcatggatga aaaggtcatg gaaatgcttg
     8581 atattatgag cgaagaagaa ttaaatgatt atgaaacaaa agtaaaggat gcggtacgac
     8641 aaagtgacag ctcatgtgca gatataatta tggatgaaga aaaaggtcta aacataattg
     8701 tttttgccag cggctggaat gaaggaacat tccctactta ttacggtttt gacaagaata
     8761 ataaactatc ccggcttgta actgatttta tggtaataga aaagtagtac tgcctaaccg
     8821 atacttctcc ctgcaagccc tatttttcag agtctttttt tgcgtttctt aaataattat
     8881 taaaaacaca tatataaaca tatacattct tgaaatatta attaattggt gataaaataa
     8941 ataatctatt aaatatgacg aaggaatact atgaataaga aaatcaacaa atatgatatt
     9001 ggccttatag tatctataat aattattagt cttttcttta ttttgtatgg cggtcgggat
     9061 gccgtgatca gcaatagcaa aacagcatat atttattcta acaataaatt ggtgggagaa
     9121 tatgttttaa ccgacgatta taaggatgta gttaacatag aatctgaaac aggttataat
     9181 ataatgcata ttgaaaatgg acaaatttgg atacacgaag caagctgtcc cgataaaatt
     9241 tgcatatatc agggcaaaat atcaaaaaac ggcgagatga ttgtatgtat tccaaataga
     9301 atgtttataa aaattgtaga cgaaaatgac gaaagtgaaa ttgatttcat agctgattag
     9361 aaagagaata ttatgaataa aatccatagg tatatttttt tatcgctttt gactgcggca
     9421 gcgctgatac ttagcataat agaaggtatg atgccattac cttacatagc tcccggagca
     9481 aagttaggtt tatcaaatat tgtaatacta tcgacagtag ttgtatttgg ttttaaggat
     9541 gctcttcttg ttgttataat aagaagtatt cttttaatgc ttgttgctac aaatcccatt
     9601 acatttattt acagtattgc gagcggtatc gtaagcacaa ttgtaatgtc attggcatgt
     9661 aagtatttaa gcaatatttt tagtttaatc gggattagcg ttttgggagc aatggcacat
     9721 aatacaactc aggtaacggt ggctgcaata ttgtttacta ccgtgaactt gtattattat
     9781 ttgcccatat tgtcattggt aagtattttt accggatgct ttgtaggata tacagcaatt
     9841 tttgtaacaa acaacttgga aaccactgtt aaaacttgga ggagaggcgt gtaatgagaa
     9901 aaggcaattc tttttatttg atattactca tacttacatc tgtaattata ggaacaattt
     9961 taggtaaggc attttcaaat atgcttccca tactaaatta tggcgaagga attggttttg
    10021 gtcctgcaac tttggatttg aacattttca ctattacatt cggattcagc gccagactga
    10081 ctgttgcagg aataatcggt ctgattgcag gaataataat attcagaaaa ctataaggat
    10141 taatatgaag aaaatagtat tggcatcaaa ttctccgaga agaattgaaa tgatgaagaa
    10201 atatgtagac ataataactt atattcctga tatcgtagaa aaggtaaatt cttttgatta
    10261 cccccaaaca acggttatga aggtagcttt tgaaaaggca ttaaatgtct atttaaaata
    10321 taaagaaaat gcactgatta tagctgcaga tacaatcgta tatttagacg aaataatggg
    10381 caagccaaaa gatgaagcag aagcttataa aatgcttagt ctgctttcag gaaaaactca
    10441 ctatgtgttt acaggtattt gtataattga tacagaaagc tcaaaaaaga tagtggatta
    10501 tgagaaaact caagttgtgt ttaatgagct tacagaagga ttaattaaaa gatacttgaa
    10561 aacatatgag tatttggata aagcaggagc atacggtatt caaggatacg gagaattatt
    10621 ggttaaagaa atacatggat gctacaataa tgtaaaaggg ctgcctattg caaaactgaa
    10681 taatttgctt tcaaaacatt tttcttacga cttgctgtaa tcaaatattg catagtgggg
    10741 gataaaatgg acaatactaa ttataccata aaatcattgc ctgcagatga caggcctcag
    10801 gagaaactat taaaatatgg agccggtgca ttgtctaact cagagcttat tgcagtaatt
    10861 ttaaggacag gaacgaggga agaaaatgtt gttatgctgt ctcagaggat tctaaaggaa
    10921 ggcggcaaag ggcttcgaaa tattgcagaa ggaagtctgg aaaagtttaa atcctacaag
    10981 ggaatcagtg acgctaaggc tgcaaagctt atggctcttg cagaaataag caaaagaatg
    11041 agcacattaa aaattgaaaa attaaagatt tcttctcctg atgatgcagc tgtgatgatg
    11101 atggaagaaa tgagatatta tcaaaaagag tattttaaaa taatgctttt agacaccaaa
    11161 aacaacgtaa aaaaggtttc tgaaatatca gtgggaagct taaacagttc aattgtccat
    11221 cctcgggaag tattttatga agcagtcgta aatttggctt cttcaataat attagttcac
    11281 aatcacccca gcggagattg cgagcccagc catgaggata ttgttttaac aaacaggttg
    11341 gatgaaagcg gcaaaatttt gggaatcaga gttttagatc atataataat cggtgacggc
    11401 gtatatttca gttttaagga agaaggctta atttaaaatg aaaggataca taaatgggac
    11461 tatttgattt ttttactcag aaaataggaa tagatttagg aacatcaaac accttagtat
    11521 atgtaaacaa taaaggaatt actttaaatg aaccatcagt ggttgcaata aacacgacgt
    11581 catatgaggt atgtgcagtc ggttttgatg caaaagcaat gcttgagaga actccaaaga
    11641 ccataagggc gataagaccg ttgcaaaacg gagtaattgc agactttgaa atcacaaggg
    11701 caatgataaa atactttata acaaaggctc ttaacaaaaa aagatttatt aaatcaaata
    11761 tagtaataag tgttcccgtg ggagttactt caattgaaag aagagcagtg gaagaagtag
    11821 catatgatgc cggtgcaagg aaggtaaaac ttgttgaaga acctatggca gctgcaattg
    11881 gcgccgggct taatgtagac cagccgatag gcaatatgat tgttgatata ggagggggta
    11941 caaccgagat tgcagtaata tcactgggag gaattgttga aagcgattct ctaaggattg
    12001 caggagatga tatcgatatt aatattaagg aatatatcaa aaataagtat aaaatggata
    12061 taggtattgt tactgctgaa aatatcaaaa aagtattagg aaatgcaagc aaggaatatt
    12121 ataaaagaat catagattca gatgaaagaa ttgacagcaa agaagcaaat gaagacaatg
    12181 aagattttga aatcaatgaa gataataaag ataaaaaaga tttaaaagac aagaaagaca
    12241 aaaaagaaaa aaaagatgaa aaagaaaata aaaaaaccga ttatgttcaa caaatggaaa
    12301 taaggggaag ggacattgtg tcgggacttc cgttaaaaat aacaataaca agcgatgagg
    12361 tgagagaagc tattcttgaa accgtaaatt caattgttaa tgccataaaa agggttcttg
    12421 aaagaacacc tcccgaatta tcttcagata tttatactaa tggaatatac ttaagcggtg
    12481 ggggagcgtt gttaaaaggc ttggatatat atcttgaaaa ggaaaccgga ataaaagtat
    12541 ttattgcaga taatccattg caaagtgttg ctttgggtgc aggcaaaatc agtgaagaat
    12601 tataacgaga aattgcgtgg caatttcttc tgatgtttga aagcggtgag caaatgaatt
    12661 ttttgaaaga gcatttcaag aaaatattat tttttcttac aattttcata ttgataataa
    12721 tgataggact gtcatcaatc ggacgatttg aaggagcaga tttaggcttt atcggcaact
    12781 tgatagcagg ctttcaaaag ctcacctata agacaggcaa tacacttaca aattcattct
    12841 attccgtaca ggatatcgtg aaaatgcgtg aagaaaatat aatgctcact gaaagtattt
    12901 ataaactcag ggagcaagta aggatattgg agaatattat caataagtcc gaagctttgg
    12961 aaacggaata cgaaatgagg aagaacctta cttatgacta tgttgttgga caagtaatcg
    13021 ctattgatga ttctaattgg ttcagcagat ttacaatcga caagggagaa aatgacggta
    13081 tacgacaaaa tgacatagtt atacaagcag tgaaaacgga tgacggcata gtacagatag
    13141 gtcttttagg cattgttact caaacttcat ccgattgggc taaggttatt acacttttag
    13201 acgagggctg taaggtaagc ttcaaaaata ttgaaaacga tgagagcgga attgtacagg
    13261 gaagtattga cggcaatgta acaggatact ttttcaacag caaagccggt gctgaaaaag
    13321 gtgataatct cgttacatca ggaataggag aaatttatat tcctgatatt tatataggtg
    13381 atgtaaaaga tgttgttaaa acaacagatg cttctaccct aagaattatt gtggagcctg
    13441 ctgttgattt tacaaagata agcagagtat atgtacttac aaaataatga ttagaaaggc
    13501 ttaataatga aaaagcttat tcttatgtct tccttaatag ttataggttt tgtattccaa
    13561 acatcattta ttggcttttt taactatttt aaaattcttc caaatattcc gctgatttta
    13621 ttggtgatat ttgccatgtt atcggatggg gtagagggag gagttctggg gattctgaca
    13681 gggattttgc atgatgccat gatttatgat gtctttggaa tatatacttt gatttatttt
    13741 attataggcg cagtaatagg agccttcagc gatgaaatgc tcagagaaaa taagcttgtt
    13801 tacagtgccg taaccggtat ttcaactgta gtgatgcatt tattattatt tcttatttta
    13861 ttctttttaa gattcagagt acatttagca aaaagtatat tgccaaatat actttttgaa
    13921 acagcattaa atgctgtgct tgttatattt gtgttaaaat ttgttatata tatttttgat
    13981 agatttaatg taaaggcatg aggtgaaatg ataaagtttt ttaaaaatcg ctttaatata
    14041 ttatacttga taatagtttt gttgcttatc attttaggtt ttagaatggc tgtactcacc
    14101 attgttgaag gacaggaata cagacaaatt gcagatataa agaaaattaa ggatatcccc
    14161 gttaaagcac ccagggggaa aatttttgac agaaacggca taattcttgc cgacaatgtg
    14221 tccagcttta ccgtgcaaat gtacacggat gaaataaaac ctgaagattt taatgagata
    14281 gcatataaac tgtcaaaaat attagatgaa aatatggaaa gaccaataga tgattttcct
    14341 attgagcttg atacatttga ttatataaat gaaagtgttg aaataattga cagcgattta
    14401 aatcttgaag aagaaattga agtaaactat taccgaaccg ctgaagaaaa agtaatagaa
    14461 aaagtcaagg aaaatattca tgaatggctg aacttcagca tttctatata cggtgagaat
    14521 ttttctccca aacaaagggt tcatgcagcc attataaacg ataatgtacc aataaactta
    14581 gagggcgata aatttgaata tgaggatgaa atgcttatac aaactgattc ggatttaact
    14641 gaagcagaaa atgaaacaac tgagcaaaaa actcccatac aaatttggct ttcagaacat
    14701 aatttaaaag aaaatataag tgctgaagaa gcagtggcac aacttttggt caacaacaat
    14761 aaatatttat cttcactttt ttccaattca aaaataagga gactttccta tgaatttatt
    14821 attcaaaagg gagtagaaaa tattaagctt aacgagtttt catttataca ggacgaagaa
    14881 tatgaaaaca tcaaaagaga tttaaatgca ttgaatgaag aaattactat ggacacatcg
    14941 gctaaagatg attttgttat tttgaccatg aaaaactcta tagataattt gcttcagaca
    15001 gtatatcagg gaaatgaaaa aattatgccc ggagcaattc ttatagaaag actgaaagaa
    15061 atttatcccg acttgccggt taaatttgta gaaaacggtg aacttggagt atttgaatat
    15121 acagatacag aaacccggaa caagtatttt aatcaacagc atatggaata cagcactaca
    15181 gcctatgatt ttataaaaga aatagctttt aaaaattatg atgtcttata caatttggtt
    15241 acggatgata acataaaata ttattcccaa atagagcttt taaaatatgt aaatccttca
    15301 atatcaataa acgaatggga atacacacct ttgcgaaaca agaaaaactg gattaataac
    15361 aatgtagaac tagatgaaga aactgatgat tactcagcaa aaaaagtatt tgaaatgctt
    15421 agagagtcct taaaaatacc tgaagaaatc aacgattacg atatgcgaaa tatgatggta
    15481 atcagggaaa ggtataacat aaaaagctat ctttcatacc accccgtgga tatttgctat
    15541 agtatttcac aaaaaaccgt tgcaatgatt tctgaaaggc atcatgaatt aaacggaata
    15601 aatatagaaa ttgaaccgct aaggtattat ccccagtatg aaagcgcctc tcatattata
    15661 ggctacctgg ggaaaattgc tcaggattat gaattgcacg aatatgtcgt tgaaaagggc
    15721 tatagtacag atgacataat aggcaagacg gggcttgaag aaaaatttga ggattattta
    15781 aggggagaaa agggtaaaaa aactgttgaa gtaaacaatg tgggaagaac aatccgctca
    15841 gtatcaagcg aagcgcctgt tcccggcgat gatctgtatt taaccttgga tcttaggctg
    15901 cagaaaaaag cagaagaatc tttgaagaaa ggtcttgaaa aaatacgtga aggcggagta
    15961 tttgaaagcg aatggggaga ttttcaattt agagacaaat atgaaaatgc ctattcggga
    16021 gcattggttg cacttgatgt taaaaccaat gaaatcctgg ctttggcaaa ttacccggca
    16081 tatgacttga atttgttttc aacgggaatt accacagaga actggaacag cctgttaaat
    16141 gattcaaaaa accctttggc tccaagacca ctttacaata ttgcaatgtt aacggcaata
    16201 cagccgggct ccacctttaa aatgattaca tccttggctg ccttggaaaa gggagtaaat
    16261 cctaatacta cggtgtattg tgcaggaacc atgaaggtag gggatagaga tttcagatgt
    16321 tggatttata acatgttcgg aggaaggcac ggctctcaaa caatgtatca ggcaataatg
    16381 aattcctgta acttttactt ttatacaaca gtcttaggtg aaaatttagc aactcaccaa
    16441 aagcataccg tgaaggttga tgcagaggat ataatagaaa tggcaagcaa gtttggatta
    16501 gattcaaaaa caggaataga gattgacata ccccaggaag cttcaggcgg agttccgagc
    16561 atagaaggta aaaaatccgg aattcgtgtt tacctaagat tatttctaga agcaaacatc
    16621 gaaagatatc ttaatgatgg ggttgttata gatgcatcga tgaaaaatga aattatagaa
    16681 gaaattgcaa gctggataga cagggatgag ctgatgacaa gagccgaagt ctatgaagga
    16741 ctattagcat taaatttaaa ccccgataaa acaaacgata attatgtacc cttagtcgat
    16801 ataataaaat attcatattt aaaccaagca acatggacag tgggagataa tttaaatatt
    16861 agcataggtc agggaaataa tgcctataca gcattgcaga tggcaaatta cgtttcttcc
    16921 atagcaagcg gcttaagaag aaatgtcagc gtagtaaaag aaataaaaac ttatgacgga
    16981 aagaaaacag attatattcc gcttagggaa tccgaaaaaa ttgaattgtc cgatgacagc
    17041 tatttagata ttgtaaaaca tgggatgaag cttgtttctt atgatgacag tgcaaaacca
    17101 tttgcaatct tccctgttga ggtaggcagc aagaccggta cggctcaaaa tcaaggaatc
    17161 aatcctgaaa caggacggcc ttatgatgat tttgcgtggt atgttgcatt tgcaccttac
    17221 gatgacccgc aaatagccgt agcatgtgta ctttttgaag gaggctcggg acgttatccc
    17281 attccaatag tgagagaagt tataggagag tatttaaaga taaacggttt gattgagtga
    17341 caaaatgcag aatagtcgtt atagggggat aaaaaatgaa tgttatatta acaggttcac
    17401 aaaaaaacaa tataggcaag acaataataa gtataaagtt agcaattgag cttgcaaact
    17461 caggcaaaaa tgttctaatg atggatttgt cctcgggaaa gataaaaatg tcagaatact
    17521 tgaatgtcaa tgaagatatt atttatgaca tagttgatgt tgtaaataaa acttgcacct
    17581 tggaacaggg tattatagaa attaacgaaa atttatcgct gttgccatgt cccagaatag
    17641 ccggcaaaat aaatgaaata aaaaaagaat catttataaa cttactccaa agtgtagaag
    17701 tctatgatta tgttataata gacgcagaca aattaacatc cgtatttata gatttttcaa
    17761 taattaataa tgcaataaca atcaataaca atgatttctc atgtataaag gaaataaata
    17821 cggataaaac aatttcttct aatgccggta actttattgt tgcaataaac aagtacaaca
    17881 aaaagaaagc taaaaaagga attatgatga agagcaatga tattgaaaag ttaactgaaa
    17941 caacaatttc agctttaatt gaagaaaata taaagtattc ggatttgaat catgaaaagt
    18001 tccttgatga taatttttta aaaacagaaa tcaaaaacat agtaaagaat ttaaaataac
    18061 ctgctttcct ctgtttttat aggaatagtg aaggattatg aaatattaac ttttgtcact
    18121 ggtaatacag ggacaaaagt ttttttattt tgtaataaag cttgtaacac attaataaaa
    18181 tgtattggaa ggggtggtga aatgaataag ctaacaaaaa aaaggctcaa tattaaaaga
    18241 aaggaacaag ttaataagaa attagtttta caggtaattt tcagcatagt tttggtagca
    18301 gccgttattg taacaaagaa tataaactcg aatctttcag aaaaaattat agaagcagca
    18361 gatgaaaaaa tgagtgcaag cattgaatta ttggaagtaa gagatactat tgcaaagggt
    18421 tttacaactg caataaacaa attacctttt gtaggtaaag aagaagcttc acccgtaata
    18481 gggacacttt atcgaaaata cggactgaac aaaacagacg atgtatcgta ttacaatcat
    18541 ggaatagaca ttaaatccga aacacaatcg gttaaggctc ttacggacgg aaaagtaatg
    18601 tcggtaggca aaaatgaaaa acattcgggc tatattgtaa tacaagatga tgatagaaca
    18661 ttgatttacg ggaaaataaa tgaaactttc gttaaagagg gggataatat atccaaggga
    18721 gatgttatag gagcccttga cgaagaaaat atgattcttc acattgaagt atgggaggac
    18781 ggtgcttcaa taaatccttc atcattattt gatttgacaa aataaacacc tttgggaaat
    18841 gcttttaatc ttagctctct tagcacacga ggtttcccat gtattttctg caatattact
    18901 gaatattgaa ttttctaaag ttaaaattac actgttcgga atcaatctaa atgcacattt
    18961 agacaatgtt tcacatgcaa aaaaaattat tttgtttttt gcaggacctt tatgtaatct
    19021 gtgtttttat tttggattta gaaatacaga atatttttgt tttgcaaata tgaatttgtt
    19081 attggcatac ataaatttat ttccgattgt gcctttagac ggaggaaata tttgcaaaac
    19141 tattttagaa atttttttgg atacaaggac ggtatccggt tacataacta tgacaaatgc
    19201 tttttttata cttttcttta taattataat atacatattc aaagactatt tgtttttttt
    19261 gctggttgtt atgggactaa aggggatagt ggaggaaaac agttacttgc ttgaaaaatc
    19321 cattatagca gtatataata aaataaatac aaaatgaaat aaccctaaac aactcttaaa
    19381 tccaaatagt cggagggata aatggaatct gaaaatataa taattagaaa aagtactttt
    19441 gatgattgca aaatatttac cgaatgggag caacaagatt atgttaagga atttttgaca
    19501 ataaacaaag agcgtacata cgaggaagtt gttagagagt ttatcttgag agagcaggac
    19561 cctacaaagg agcaatatac gattattctg aaaaacacag gacccatcgg aagagtctat
    19621 ctttctaata tatcaagaga atttgacttc attgatatta cacggatata tatcggagaa
    19681 aaggagcatc ttggaaaggg ttacggaaga gagtgcatgg aattgctctt agactattgc
    19741 tttaatattt taaaaatgga aagggtaaca ttagaccatt atacaggaaa taaagctagt
    19801 aatctgtatt tgaaattagg ttttaaatat gaaggaataa tgagacatgc tgcaaaaaaa
    19861 gacggtaaat actatgattt gcacttaatg tcaatgttga gggaagaata taaacaatct
    19921 atttaaaaat aattgaaaat tcaaaaaatt tgtattgatg tagtaagcgt gaaaagaatg
    19981 attaattcgg atgcaaataa tagaaaaggc gaagaagtta taatatataa ctacaaggta
    20041 taaaagatgt tgcaatgaag tgcattgttt attatagaaa ggaatttgag acaagcatga
    20101 ataaaaaacc gatagttgca ataatgtatg attttgataa aacactttca ccgaaggata
    20161 tgcaggaata tggattcatc tctgagttag ggatgaaatc cgaggatttt tgggagaaaa
    20221 gcaggttaaa tatggttaat cacaacatgg accagatact ggcatatatg ttaaccatgt
    20281 tgcaggaagc agaaggcaga atgcttttga cccgaaatat atttaatgaa atgggcagga
    20341 atgtgaaatt atttgaaggg ctagaaactt ggtttgacag aattaactta tatggagaaa
    20401 agttgggatt ggaaatagag cattatataa tatcttcagg attgaaggaa ataatccagg
    20461 gaactgagat tgctcataat ttcaaagaaa tttatgctgc cgaatactgc tataatgaaa
    20521 ataatattcc tatatggccg gcaatggctg taaattatac cagcaaaact caatttttgt
    20581 tcagaataaa taagggtgtg ttggatgtta cagagcataa aggattaaac gaatccactc
    20641 cggataataa gaaaagaata cctttctgta atatgattta tattggtgac ggtttgactg
    20701 atgtgccgtg catgaagtta gtcaaaatta acggcgggca ttccattgca gtctatcatg
    20761 atgacaggtg tattgcagac agcctgattt cacaaggcag ggttgatttt gtcgttagtg
    20821 ccgactatag agaaaacagc gaaatggaaa aaacagttca aacgattctt aatcaaatag
    20881 cagctttaaa taaggtgcta aaactgcata caaagcatat gaagaacctt ataacggact
    20941 gagttaatgc ataaatgaag aataaaaaaa ctatttgaaa ttttttaaaa aagtagtata
    21001 atataaatac atttgtaaat gaaaatttat aaatgcctct taaatttttt gcctcaagcc
    21061 gtgaatgtat tcacggcttg cccttttata ggcatgggga aggcgtcctc gtcatgtatg
    21121 taaaagtgaa aataaaatag tcaaaataca aaatataaaa attataataa aaatatgttt
    21181 ttttagaaat tcccatattt ttaaaatcaa tataatcccc ccgtatatta tatgtgaata
    21241 aatgaacgct aattataaga atgcataaat aaattaaaaa tcaatttttc tgtttcaggt
    21301 attttttgga tggcttaatt gactgaaaaa ttactttaca agagttaatc aatctattat
    21361 aatattatag gtaatgcggt tgtggcggaa cggcagacgc actagcttga ggggctagcg
    21421 aatgtatatt cgtgggggtt caaatccccc caaccgcact aagatagttt cgggtaatcc
    21481 ccgaaactat ttgttttatc tgtgtaattt tgttacggtt cattcataga atgataaggg
    21541 taaagattga taatgtattt ttgcttgtaa ttatactgct tttgaattat aatgattata
    21601 taataaagcg gtgaggggaa aatggaagtt gcaattttaa aatcatttac ctatgaaaac
    21661 agcggtggca atggagcagg tgttgttgtt ttgaatcaac agataccata tgataagaag
    21721 caagaaattg caaataaagt aggattatca gagacggcgt ttatttataa acgggatgaa
    21781 aacgattatg atgtgagttt tttcactccc gtttgcgaag tagacctctg cggacatgct
    21841 acaattgcag ctttttatta cttgggtaaa attggacaaa ttaaaggtaa cgatgaaaca
    21901 aaatgcgtgt atcaaaatac gaaggcaggc agactcaaaa ttgaaataac atttaaagga
    21961 caagatgttg aatatgttct tatgcagcaa agcgaaccac gtatatacgg tgagcttaaa
    22021 gagaaaaaac aaatagcaga ttcgctgaat atagatgaaa gattaatagg tctgccgaat
    22081 tatgatattg ctcctacaat tgtttctaca ggtttaaaag acattttggt accggttagc
    22141 tcgaggaagg tgctaaatag tcttgatgtt gatttcaaga tgatagagga tatttcttat
    22201 gaaaatgatg tagttgggta tcatgttttt acaattgaca atgaaagagt ttatgcgaga
    22261 aatttcgctc caagagtggg tataaatgaa gaatgtgcaa caggtacatc caacggagca
    22321 ttgggaagct tacttttcag taaagggttt tcaaatcaaa tggaagtaat tcaaggtgaa
    22381 tcaatgaacc aaaaaagtct tatctttgta agaattgatt ctcataatga tattatggac
    22441 gtatacgtag gcggaaaagc ccacattgaa aattatatta aatagcgagg gatggtatgc
    22501 aaataaaaga aatagttgat attgtaaaag cagatgttct tacaaactat catgacggta
    22561 ataccgaatt taaattcggt tttgccagtg atttaatgag tgatgtactt gcatatggac
    22621 gcagcgattc gttgctgata acagggttaa acaatccgca ggttattagg actgttgaaa
    22681 tgctggatat taatgccgta ttgtttgtaa gggggaaaag accttgcgaa gaagtaatag
    22741 aattagcaaa ggaaaacggt attacaattc ttgcaaccga ttacactatg tttaaaacat
    22801 gcggattatt ggttatgaac ggaatggaag ggctagaaat aaatgactga taaattttta
    22861 aaaagataca gctacaatat aacagcaaat gattttatgt ctgcaggcaa aagtgcaagt
    22921 gccataaaaa gcaatttaaa atcattggga attgattcga atactataag aaaaatatca
    22981 ataatatcat atgaaactga aataaatatt gttatacatt cttacggcgg gaaacttcac
    23041 tgcgatatat atgaaaataa aatagtaata atttcggaag atacaggtcc gggaataacg
    23101 gatttagata tggcaatgac agaggggtac tccactgcga cagacagtgt aagagagctt
    23161 ggatttggtg caggtatggg acttccaaat attaaaaaat attctgacca attagaaatc
    23221 aatacaagta gtaccgggac aacaataatt gcgacaatct atttgtagga gggtattatg
    23281 aacttttcaa ttatgtttga caaagataaa tgcagaggat gtaccaattg catgaaaaga
    23341 tgtcctactc aggctataag attaatagaa gggaaagcat ttataaatac ggcaaaatgc
    23401 attcattgcg gtgagtgtat aaaaatatgc ccttataatg cttattctcc cgaatccata
    23461 gagtggtaca atcaaatgca tgatacaccc aaaaaattca aaattgcaat tcctaccact
    23521 tctttatacg ggcagttccc caggggaaca gatatatgca aggtccaaaa tgcaattttg
    23581 aaattaggtt ttgattttgt gtatgatgca agctgggcgg cagatttggt tgcaaaagcc
    23641 ataaaactta aaatagataa agaaaaaaca atgagacctt atatatcaac atattgtccg
    23701 gcagcggtta ggcttattaa atacaggtac ccttcattga tggataatct tattttaata
    23761 agggagccta tggaaattgc aggaatactt gcaagggaaa gagtcaggaa aaagttcggt
    23821 ttaaaggatg aagatataaa agtattttat atatcaccat gtcctgccaa gttgttggcg
    23881 gtaacgaatc caatcggaga ctacaaacct tcggtagatt gggtaatacc tcttaacaaa
    23941 atatacggag acctgtacag ggaagttata agagaggata atattacatg ctcatacccg
    24001 tcactattag gtatgaaatg ggcagtggca ggcggtcagt cggaacatgc ggaattgaat
    24061 aattatatag ccgttcacgg catggaaaac atcattgaga tcttagagga aatcgaaaac
    24121 ggtaaattaa gcgatataga ttttgtggaa gcttcagctt gtgttaacgg ttgtgtaggc
    24181 ggaaccttta atgttgtaaa tccgtttatt gcaagcagca gcattaatta tatttcaaac
    24241 agtctgcctg atgaatgttt aaatcctgat atcgacagat ttgacgaaat gtataaaacc
    24301 ggcttcttta attataagct gaaggaagaa gaaaaatttg acaaacttaa cttaaaggaa
    24361 gctgttgaaa ggaatgaaaa aataagggaa atccttgata aacttccggg attggattgc
    24421 ggctcctgcg gagcacccac atgtttggct cacgctgaag acgtttataa taacgagtct
    24481 gaattttatg actgtgttgt actgagagca aaaaaataag aggaggggaa aatgaactgc
    24541 ttagaatttg cagaaaaact gcagcttaca ccactgtgtg aaaaagaagg agatattgaa
    24601 attgaaggtg tgtatatagg tgatttacta agtattgtaa tggcaaaagc aaaaaagaac
    24661 tatgtatgga taactattca aacccatatt aatattgtag ctattgccga gcttttggag
    24721 ctttcttgta ttatagttgt tgaaaatatg gcagtcgaca atgatacttt ggataaagca
    24781 aaggaattaa acatacctat atttaaaaca tcagaaagtg cataccaagt agcatgcaga
    24841 ttgtcagata tgggaataaa atgaaatttt attatgattt gcatattcat tcggatttgt
    24901 ctccgtgcag cgcatgcgat atgactccaa ataatattat aaatatgtcc ttaataaaag
    24961 ggctggatat aatatctgta acagaccata attcagcggg aaatttgcgt gcagtaatgg
    25021 aattaggcaa taaaacaggc ataaaggtag tacccggatt agaagtaaca acaaaggaag
    25081 aagtacacgt gctttgctac tttaaagaat tggataatgc aatagaattt ggcaatatta
    25141 tttataattc attacctttt ataaaaaata atcctgaaat attcggtgag caaaatatat
    25201 ataattctaa ggatgaaata acaggcacct tggaaaaatt attgattaat gcttcgcaat
    25261 ataccatcga agatattttt tctttagtca agaaatacaa tggagtgatg attcctgccc
    25321 atattaacaa aatgacaaac ggtattctcg gggtattagg ttttatgcct atcaatctga
    25381 aaatagagac tgtggaagta tattcaaaaa tgcctattaa cagtaaaata gcagaaaaat
    25441 ataaggtgat ttataattcg gacgcccatc agcttgttga tatagctgaa gcggttaatt
    25501 attttgatct ggatgatatc gaagagattt atgagtattt atttgaatga atgtccacgt
    25561 atttactaaa aggagggggg acacacctat agaggtatgg ggacacacct ctttctattg
    25621 ggataaccta tgtcggtcag aggaatgtcc ccaattgcat tccactgcgt aatatcttgg
    25681 ttggaggaat tatgaaagaa atttcaatgc acatattgga tattgcaatg aacagcatta
    25741 aggctgatgc ttcattaatt gaaataaata tagaagatag tataaaaaat aaccggttaa
    25801 gtataactat tgcggataac ggcagaggaa tgagcgaaga tgttataaac agagtatcca
    25861 atcccttcta tacaactcgc actaccagaa aagtaggatt aggaattcca atgctgaaag
    25921 aagcatgcga aagatgtaac ggctatttaa aaattgaatc aaagataggc gtcggaacaa
    25981 aggtttgctg ttattttgaa agggataata tagacagagc acccctcggt aacatggggg
    26041 aaacaattat gacaattatt aactccggtg ataattttga actaacttat tctcataaga
    26101 ctgacagggg agaatttatt tttgatacga gagaagtaaa agaaatgctt gaaggtatta
    26161 atattaacga catcggtgtt ttaatgtgga ttaaggaata tatcaatgaa aatgtaaacc
    26221 ttgtatcggc aggtaaaatt tgataattta tggtcttgca taatttgata aataatgtta
    26281 aaattataaa aataaataaa aaaaggtatt taaaagtcga aaacaatctg ttataatctt
    26341 tatgtttgag attataactt ttttataatt cataattatt aatttcacga ggtggtataa
    26401 tgagcacaaa attgtttacg tttaagggtg gaattcatcc ccctcaaagt aagtctttta
    26461 cgcagaatgt gccaattgaa gacgcaccgg ctcctgatat tgttgtaatt ccattgtcgc
    26521 aacatattgg agccccatgt gatcctttgg tggctgtagg agataaagta aaagttggac
    26581 aaaaaattgg cgaatcaaaa gcctttgttt cagcacccgt tcattcgagc gtgtccggaa
    26641 cagtaaaaaa gattgaaaat cgacaggtgc cgggcggagc gaaggtaaat tgcgttatta
    26701 tagaatccga cgggaaatat gaagtgcatg aatcagttca gccaaaaggc aaattagaag
    26761 atctttctaa agaagacata ctttctataa tcaaagaagc aggtatggca ggaatgggag
    26821 gagcaacttt ccccactcat gttaagctaa gtcctccaaa agacaaaacc attgatacct
    26881 tgctggtaaa cggttcggaa tgtgagcctt atttgactgc cgaccataga attatgttgg
    26941 aaaaacctga tcttgtattg cttggcacaa aggctgttat gaaggcatta ggagtaaata
    27001 aagcatatat ttgtatagaa gataacaagc ctgatgcaat tgaaacattg cagcaagctg
    27061 ttggcaatga ggaaggtatt gaagttgtat cgatgatagc taaataccct caaggcgatg
    27121 aaaaaagatt aattaatgcg gtaaccggaa gagttgtacc ttcaggagga cttccaatgg
    27181 aagttggatg tgttgtagaa aatgtcggta cagttgctac aataggcaat gtaataaaaa
    27241 caggtatgcc ctgcattcaa agagttgtaa ccgtaacagg aagtgctgtt gaaaacccta
    27301 aaaacctata cgtaagaata ggaactctgt ttaaggattg tattgaaatg tgcggcggtt
    27361 acaaagaaga gccgggaaaa attatcagcg gcggaccaat gatgggaatt gcccaatata
    27421 ctgacgaagt ccctgttata aaaggaacat cgggaatatt ggttttaaat aaaaaagaag
    27481 caaatgtacc ggagcattca aattgtattt tatgcggcaa atgtgttgag gcttgtcctg
    27541 tatacctaat gccttacaaa atagcaagat tttcattatt gagaaatttc gacggtgctg
    27601 atgaatatca tgcaactgat tgtatagaat gcggtgcttg ttcgtatgta tgtcctgcaa
    27661 agagaccgct taaagaatcg ataagtgttg caaaaaaaga aattttaacc agacgcaaaa
    27721 aggctaaata ggaggaaata ttgatggaaa ttaaattgat tggttcatcg tcacctcata
    27781 ttagatccga tgaaacaaca caaaaattaa tgcttgatgt gataattgca ttaattcccg
    27841 cattggcagc aagcatatat tattttggat ataaagcaat gatattggta ttagcatctg
    27901 taatttctgc agttgctacc gaatattttt gtcaaaaatt tatgaacaga ccaataacag
    27961 ttaccgattt aagtgcggtt gtaacaggaa tactcttagc atttaattta cccgcagaag
    28021 caccatggtg gatagcagtt atcggctctg tatttgcaat aggaatagta aaacagatat
    28081 ttggcggaat agggtttaac tttttaaatc cggcattggg agcaagagca ttcttaatgg
    28141 catcatggcc tcatcatctg tcaggaggat ttattgaccc ggcaattgat gcagttactt
    28201 ctgcgacacc tctttctatt atcaagggtg cagcatccgg acaactgccg tcattgtggg
    28261 atatgttaat aggaaatata ccgggagtta tcggagaaac ttcggcaata ctattattag
    28321 ccggtggaat atatttaatt tacaccggaa caatcaaatg gataattccg gcgttttata
    28381 taggtacagt tgcagcaata gcattacttt ttgatgctgg caatttatca taccacatct
    28441 taggtggagg tttgatgttg ggagcatttt tcatggctac cgattatgca acaagcccca
    28501 ttaccgataa gggaaaagtt atatatgcag ttggagccgg aatgcttaca atgtttataa
    28561 gaaaaatggg aggatatccc gaaggagttt cttattcaat acttctgatg aatattttga
    28621 ctccaatgat agataaatat gttaagcctc gcgtattcgg agttgcaaca ggaggtaaaa
    28681 aaaatgaaaa ataattttgt aaaacttgca ggagtattgc ttattatagg tgcggtatca
    28741 gcaggagtat tagcatggct gaatgacgta acaagtgata gaattgcaca aaatgaagca
    28801 atggcaagta tggacccggc tatattggaa gcagttatgc ccggatctgt aatgtttaat
    28861 gattatgaag atactgcatt ggtagataca attaaagctg ataataacaa atttattaat
    28921 cttcttacag ctgttgatga atcaggtaac gaacttggga aagttataag aacatggagt
    28981 actgttaaag gcttcaacgg agacttggaa ttatatgttg gtttcacccc tgatggacaa
    29041 atatccggaa caagtgtaat atcacatgct gaaactgaag gagtaggctc taaagttact
    29101 ctacttgcat ttacagaaca gtttattggc aaggatgcaa gcggcgaaat ttctgaaggt
    29161 gactatgatg ctgtttcagg cgctacgtat tcttctaaat catttttaag tgccgtaaac
    29221 aatgctataa atgtttatgc ggaatatgtg aaataggagg atgacatgaa taaattagaa
    29281 atatttaaaa atggagtaac aacagaaaat cctattttag ttcaacaggt tggtatgtgt
    29341 gctaccttgg caattacaac atcgcttatt aacggaatag gaatgggtgt agctgtaata
    29401 gcagttttga ccggttcaaa tatagtaata tctttattgc gtaaatttat acccgatgag
    29461 gttagaatac cgtcatttat tatagtaatt gcagcattta caaccataat agatttattg
    29521 atgcatgctt atacatttga attatataaa gcattaggcg tatttatacc gttaatagtt
    29581 gttaactgta ttatccttgc ccgagctgaa gcatttgcct caaaaaacag cttgtctgac
    29641 tctataatag acggtttggg aatgggatta ggatacttag ttgctatgat tttactcagc
    29701 tctgtaagag aaatattagg caacggtaca attttgggat ttagaattct tccggaagct
    29761 tatgagccta tgatgatggc aattcaaccg ccgggagcat ttattctcct tgggttgttg
    29821 ataggtatag taaactatct ttcaagaaga aagaaagctt aggaggtagg gaatggaatt
    29881 agttagttta tttataacat catttatagt taataatatt atattcatgc agtttttggg
    29941 aacatgtcct ttcctcggag tatcaaagaa aattgatact gcagccggta tgggtattgc
    30001 ggttatattc gttataacct tggcatccgt aataacatac attattcatt atgctatttt
    30061 ggttaatttg gaattggaat atttgcagac aatagtattc atattgataa ttgctgcatt
    30121 ggtacaattt gttgaaatgt ttcttaagaa atcagctccc ggtttgtatc aggcgttggg
    30181 aattttcctg ccccttatta ctacaaactg tgcagtctta ggtgttgcca taaataatat
    30241 tatttatgag tacaattttg ttgaatcatt agtttattct gtaggtatat ccgcaggttt
    30301 cttgctggca atagtattgt ttgcaggagt tagagataag gtcgattatg ctgatttgcc
    30361 tgaatcttta aaaggctctg ctatttcatt gattactgca gggcttatgt cagttgcttt
    30421 ccttggattt tcaggattgc agttatagga ggtaaaaaga tggttatagt taaagcagta
    30481 gcagtcactg gcggcatagg tgccatatgc ggattattat tggctcttgc agctaaactg
    30541 tttcatatag atgtagatca aagaattacc gatgtaagaa atgctttgcc gggtgcaaat
    30601 tgcggtgcat gtggatatgc aggctgtgat gctatggctg aagcaatagc agctggaaga
    30661 gctcctgtag acggatgtcc ggtggcaaat tatcaggctc atgcaaaaat agccgatatt
    30721 atgggtactt cagctgaaga gggagaaaaa aaggttgctc atgttttatg tcgtggaaat
    30781 gactgcctgg cttcaaaaaa acatgaatat catggagtaa gggattgtaa ggcagcagcg
    30841 gctgttcaag gcggagataa gtcctgtgat aagggatgcc tcggttacgg aaactgtgtc
    30901 agagtatgcg attttgatgc tattgacata attgacggaa tagcagtaat agacaaagaa
    30961 aaatgtactg cttgcggaaa atgtatagaa gaatgtccaa aagccgtaat agaatttgtt
    31021 ccgtataaga ataatgttat tgtaggatgc aacaacagag acttcggtaa agcagtcaaa
    31081 gatgtttgca aaatcggttg tatagcatgc aaaatttgtg aaaagaactg tccttacgat
    31141 gcaatacacg ttacaaacaa tttagccaag gtagattatg acaaatgtac gcattgcatg
    31201 atttgcgtag aaaaatgtcc cactaaggta atagaaggag atttatcaat aaaagataaa
    31261 gcagtataac aagcatcata tctactcagt gattgagaat caatgggtag atatctattt
    31321 tttgacaaaa aatgattttg gcactataat atacaaaatg tataatctgt gataaaataa
    31381 accataggaa gataaaaaca ggagtatatt atgtattata ataattttgg cgaaagaaga
    31441 ttatctatgc tgatggattt ttatgaactt acaatggcaa acggatactt ttctaacgga
    31501 atgaaggatg aaatagtata ttttgattta tttttcagaa aaaatcctga tggtgcaggg
    31561 ttttcaatag ttgcaggatt agagcaaata attcaatata taaaagaatt aaaattttat
    31621 gaagaggata ttaattttct aagaagcaaa aaattattta cggaagaatt tttgcagtat
    31681 ttgaaagatt ttaaattttc aggtgatata tatgcaatac ctgaagggac tcctgtattt
    31741 cccaatgagc ctttaattac ggtaagagcc aagacaattg atgctcaaat aattgaaacc
    31801 atggtgcttt taacaataaa tcatcaaagc ttaattgcca ctaaagcaaa tcgagtggtt
    31861 agagcagcaa aaggtaagcc aataatggaa tttggcgcaa gaagaagtca aggctatgat
    31921 ggagcaattt atggagcaag agctgcttat atcggcggag taataggaac agccacaaca
    31981 ttggcagatg aagcctttgg agttccggca ctgggaacca tggctcattc atgggtgcaa
    32041 atgtttgaaa gcgaatatga agcatttaaa tcttatgcac aaaattatcc tgatgcttgc
    32101 aatttactgg ttgatactta caatgtagta aaaagcggaa taccaaatgc aatcaggata
    32161 tccaaagaag tattggagcc tatgggaaaa agacttaagg gagttaggat tgacagcggc
    32221 gatattgcat acttaagcaa gaaatgcagg aaaatgctta atgaagcagg gttgcatgac
    32281 tgtgcaatag ttgcatcaaa cagtcttgat gaatttatta tttctgattt actggaccag
    32341 ggagctaaaa tagattactt tggcgttggg gaaagattaa ttacagctaa gtcagagcct
    32401 gtgttcggat gcgtttataa gttagtggcg gttgaaaggg acggaaagat tattccaaag
    32461 attaaaatat cggaaaacga agaaaaaata actaatcccg gctacaaaaa ggtatggaga
    32521 ttgtatgaca aaaaaacaaa cagtcctatt gcagatgtaa taacactggc tgatgaaata
    32581 atagataatt caaagcctta tacaatattt gatgaaatac atacttggaa gaagaagaaa
    32641 gtaacaagtt tttatgcaaa aaatcttcag gtccctgttt ttataaacgg cgaatgcgtt
    32701 tatgaaagtc caagcttaaa tgaaataagg gaatattgca aaaagaaggt aggcaactta
    32761 tggggagaaa taaaaagatt ttcaaatcct caccattact atgttgacct ctctaaagaa
    32821 ctatgggact gcaaacagca cttaatatca cagtacagct tcaagtaagc agatgaccca
    32881 aatctatgat ttggtgtcag tcgcttatcc tgagcgaaag cgaaggatct tcagatgacc
    32941 caaataaaaa aagggcgttg cccttttttt tataccgaat aattatcgaa atagccttgg
    33001 atatatacta taggtgtacc tttatctccg cttcccgatg ttaaatcgca tagtgagcct
    33061 ataaggtcgg taagtcttct cggagtagtt ccttgagatt ccatcctgtc ttttaaatca
    33121 cagtccttat tcataatata atcacaaata gcttttttaa gttcatcgcc tttaaggtgc
    33181 ggaaattggt tgtctgccaa atatttaagt ttaaattcat tgggagtacc gattaatcct
    33241 tctgtatatc ccggagatac aaccgggtct gccaactccc aaattttccc gacaggatct
    33301 ttaaaagcac catccccgta tatcattact tcaattttct tgcctgtaag ctcaaaaagt
    33361 ttttcttgaa ttttttcaac taaggactgg caatctctcg gaaaaagctt tacggtatct
    33421 tcagtagcct tatttgttcc taacagtccg tacttttcat tgaaaccgct tccttctaca
    33481 gattgattca aaatatcatc caagccgtat acggtttcag cgccatttga tttcaacaat
    33541 cttttgcttc tgtttcttgt atggatgtca caggtaagaa cattctttgt ataattcaaa
    33601 atacttatgg gattatttgc aaatatgact tcaacttcac atccgctttc tcttataatt
    33661 tctgaatagt atttaatata atccactccc gtgaagatat gttttgtatt cttaaagtgg
    33721 ctcctgaatt cattctcttt aagtatatct gtccaaggat taactccttc cttatcaagg
    33781 aggtcataat caactaaggg attacccact tcatctgaag ggtaactgaa catcagatat
    33841 atttttttga atgatttagc aataccttta agacaaacag caaatctgtt tctgcttaaa
    33901 atcggaaata ttagacctac agtatcacta tcaaattttt gttttacatc cagcgctatt
    33961 tgttcaatat ttgcaaaatt accttgagct cttgctacaa ttgcttccgt aatacctact
    34021 atatccttat ctcttaatga aaagccttca gtcttggaag ccttcagcac agtttcaaca
    34081 gttattttta ctatatcatc accttttgta ataattggag ctcttaatcc tctgacaaca
    34141 gttcccacta ttctttccat ttttgtctcc ttaattttga attcaaatct atttatcata
    34201 agtgcaagcc gcaaaatcgg cttgcttcac accgatacaa tgaatgcatc agtgcaaaag
    34261 ccagagcttt tgcttcattg tttgcattca ttataataca ttttaagaca tgtgtaaaat
    34321 attattatat attactggac aaatttataa ttattaatta caatatgttt aaaaatcaaa
    34381 atgttattat gattccacgg aggaaacgtc gtgataaaaa taggtatgcg caatataaaa
    34441 acaacaattt cagtctttat atgcttattg gtttttgaac ttattaacag aaataatccc
    34501 atatttgcat gtatagctgc agtaatctgt gtacagaata cgattgttga ctcaaaggaa
    34561 ataggatttt caaggattat cggaacaata ataggaggcc tggcgggagc ctttacattg
    34621 tttatcgtaa atactttttt taatcataaa attttgattt ttattatacc attaggaatt
    34681 atgttgttaa ttcaaatctg cgtaacaatt aacatgaaga aagcagtagt tatttgctgc
    34741 gttgtatttt tagttataac cttgaatcat gatgaaggtt atatcctata tacctttaac
    34801 agagttttgg atacctcaat cggtataata attgctcttt ttgttaataa atatgtaaaa
    34861 attcctgaaa agctaaagtc aaaaattggg gataaggaag agctgataga aacacacgaa
    34921 aattctgagc cttagtaaag atgtctgcaa tgtatataga tgacataaat ataaggacaa
    34981 cttcataaaa ttaataaacc gggttaacgg attatttagt aatggaggtt gttttgtgac
    35041 tggagcagat ttaggtataa tattagctta tacggcaggg ataatgcttg tattcatgac
    35101 atcctggata ttttttaaac cgcttaaatt tataggaaaa ataattttaa attcagtgat
    35161 aggcgctata gtcttaataa tattcaatta tttcggacag tataccggtg ttcatatagg
    35221 agtaaatgaa ttcaccgcac ttattatagg aatattaggt gtacctggat tttttgctat
    35281 attgttggta aaactgctta tatagaatat tcaagtttta ttggacaatg gtaatgtgct
    35341 gttatataat atgtacagga aggtgattga ttgtatgtgg cttgaatata ttgtactatt
    35401 ttcaattgct atgacgtttt tctcaataac agtggtactt acagttgaaa ctcttgatac
    35461 cacaaataat atttctagaa aaagaaataa aaaactataa tcttaaagga gctgagtact
    35521 aatcccaata aatagaagga tatattttat taacgggaaa aaaagatgaa aaaacttaat
    35581 attatttcag gcattaaaca gtatcgaagt gaagtagata caaatttcca catgccggga
    35641 cacaaaggaa aaccggaaat tttaggtgaa ctcgaaaaaa atcttaattt ttttgacatt
    35701 acggaaacac ttggaactga taatttacat tatcctacag gctttttaaa ggatgcaatg
    35761 aattatattt ccaaagtata cggtactaaa aagtcgtaca tggttgttaa cggtacatca
    35821 tgtggtatta tctcggcaat tatggcatgc acaaaccccg gagataaaat acttgtgcaa
    35881 agagattgtc acaagtcggt ttacaatgct tgtatattag gagatttaaa actgttttat
    35941 ctttatccgg attttaataa aaagtacgga ttaaacttta gtattagctt agataagctt
    36001 gaccagatgc ttacagataa tccggacata aaaatggtgg tgctaactta tcctacattt
    36061 tacggtattt gttttgatgt aaaaaaagca gcagaaatag ttcataagca cggcaagatt
    36121 ttgatgatag atgaagccca tggctcgcat cttcaatttt gcaaggagct tttaccgccg
    36181 cctgcggaaa actccggggc agacatagta gctcaaagta cacataagac attgccatgc
    36241 cttactcaag gctcaatttt gcatatttgc tcagacagag taaatgtcaa caatattgaa
    36301 acaatgctaa ggatgctgca aaccacatct ccgtcttata ttttgatggc atcaattgaa
    36361 aatgcaattc attggatggc tgagaacgga gaagaaagat taaaaagaaa tattgaagtt
    36421 ttcaaaagaa gaacattaga gcttagaaat atgggaatca atgtattgga agatgacttt
    36481 ttaatcagcg aaggtttgta tgatttcgat gcaactcgtg cggttatcag catgagcgag
    36541 cttgggataa caggtacgga gcttcaggat atattaaggt ataaatataa aatacagatg
    36601 gaatttgctg acttgcagta tactgtcggt tatgttactg ctacagatga gcctgaagat
    36661 atcgacagac tttttgacgc tgtaaaagaa atatatatag aggaatccaa aagcaaggaa
    36721 aaaagagaaa ttgttcaaat cgagccattt cctaagctgg agcatgaaag aaaaatgagg
    36781 aaggcattct atgccgaaaa catgcttctt catatggacg atgctatagg aaagactgcg
    36841 gcagaattca taataccata tccgccggga atacctcttg tttgtcccgg agaaataatc
    36901 gttcaagata caatagatta tgtaaaaatt atgttggaaa atggcattaa cgtaaatggt
    36961 gttaacaaaa ataatcaagt gagagttgtt aaatggaagg aagatttata gtattggaag
    37021 gctccgacgg ttcaggcaaa agtaccatag cagaaagaat tggaagatat tacagcgaaa
    37081 aaggccgtga tattgaattt accagggagc cgggaggtac ttcaataagt gaaaaaatca
    37141 gggaactgat tttagacaga gataacactg aaatggatta cagaaccgaa gctttgcttt
    37201 atgccgcatc aagagcacaa ttagtgtctg aaaagataat accctggctt aaagcaggga
    37261 aagtggtaat aagcgaaaga tatgtttatt caagtttagt ataccaaggc cttggaagag
    37321 gccttggtat tgatgaaata aaaaaaataa atgattttgc aacagccgga attaagcctg
    37381 atttggttat gctattggat gttgagcctg aaaaagcatt gaaaagaaaa ctttgcattg
    37441 acgggggaga caggctggaa aacgaggata tttcttttca ccgaagcgta tatgaaggat
    37501 ataaaaagct ttcatcacat tttcctgaaa taataaccat tgatgctgaa aggtctattg
    37561 aagaaatatt tgaagaaata aagggcttat taacaatata aggaggttct tatgaaactg
    37621 ataactgcag ttgtacaaaa tgatgatgca aataagttga tatcggcatt gagagaaaat
    37681 ggcatttcat ccactaaaat gtcatcaaca ggcggctttt tgagctcagg aaatacaact
    37741 gtgatttcag ggattgaaga tgatagagtt gatgaagcaa ttgaaataat aagaaagata
    37801 tgcaagacta aaaagaaagt tactgctgcc atacctccaa gttcattcag tacggtagga
    37861 ggatatttac cgcaaactat tgaagtaact gtagggggag ctgttgtgtt tgtaactccc
    37921 gttgatagat ttgaaaagat ttaagggggc ttgtatgctt tacagccaag tagcaggaca
    37981 agaagaaata aaaacatctt taatcaagtc aatagataac aatcaagtat ctcattgtta
    38041 tctgtttgaa ggacctgaag gcatgggtaa atttgaattg gcattagtat ttgcccagtc
    38101 gcttctatgc ttaaattttc ataaggagcc atgcaataaa tgcaagaatt gcataaaagt
    38161 aaattctctt aaccaccctg atttgcacat tataaataaa gacgggaata gtattaaaag
    38221 agaagatatt gatttgctga ttaatacagt atacaataag ccttatgagg ctaaaagaaa
    38281 gatttttatt ataaaggatg ctcatttaat gacaccgcag gcagctaatg ggttcttaaa
    38341 aaccttagag gagcctccgg gtgatacagt tataatactt ttgacaatta attcaaattt
    38401 gcttctccct acaattgttt cccgctgcca ggaaataaaa tttaaaaata taagcaaaga
    38461 ttcaataaag tcatatttaa aagattatga ttcagatagg gttgaattag cggcaaatta
    38521 ttcaaaagga atactcagta aggcaataaa tataattaag ggcagtgatg acactcttaa
    38581 gaaacgagaa gaaataatta aaatttttga taaaattgta aattcggatt ctgaaataat
    38641 ttttgaattg gaaagttact ttgacgataa aaaagatgat ataaattcaa taatagaaat
    38701 aatgataatg tggataagag atataatatt tgtgaataac aacatggata gacttgtaat
    38761 aaacaaggat tatataaatc ttttaaaaac tcacgggaaa aatatgaaga aagatacaga
    38821 tataattgaa tatatgcaaa atacaagcga taatataaag agcaatgtta actataaatt
    38881 gactgtagat aatatgcttt taaagattca ggaggtcttt aaatgataaa ggtagttggc
    38941 gtaagattta aaaaagccgg caagatatac tattttgatc ctgaaaaaaa gtggattaac
    39001 aatggagatt ttgttatagt agaaacaata agaggtattg aattcggaca ggttgttgta
    39061 ggtccaaaaa tggttaagga agaagatatt gtacagccat taaaaaaagt actaaggctt
    39121 gcaacagagg atgatattaa aagaaaccga gaaaataaag aaaaagaaaa agaagcttat
    39181 gatatttgct tcaaaaagat agaagaacat ggtttggaaa tgaagctcat tgatatagaa
    39241 tatactttcg ataataacaa agtaattttt tatttcacgg ctgacggcag aatagatttc
    39301 agggaattag taaaagattt agcggcaata ttcagaacaa gaatagaatt aaggcaaatc
    39361 ggtgtcaggg atgaagctaa aatgatagga ggtcttggac cttgcggcag acatatgtgc
    39421 tgctcatcgt ttttgggtga attttatccg gtatccatta aaatggcaaa agagcaaaaa
    39481 ttatctttaa atcctgccaa aatatcagga atatgctcca ggctcatgtg ctgtttaaat
    39541 tacgagcatc aggtatacga agaaaatatt aaaaagcttc cggatatagg agataaagta
    39601 caggtgttgg gaacaaataa aacaggtata gtaatagaca ttaatccttt atttgtaact
    39661 gcaaaagtta atgtagaaaa aagtgacgga actattgaag tagaagaaat tcaaggttca
    39721 cgactgaagg ttgtggaaga aggtgtcctg aaacattacg acgcggaaga tacggacata
    39781 gatatcgaag aattaaagga attggaagat taataatttc acaccttata taaggtgtga
    39841 aattattatg aaaaaacact ttaaaattat tttaaatagt gttacaataa gtggtgatga
    39901 ccatattggg ggaggtgaat ttaaatggct tacataataa cagatgcatg tataagctgt
    39961 ggagcatgtg aacctgagtg ccctgtaggt gcaatttcag ccggtgatga caaatatgtg
    40021 atagacgctg caacttgtat tgaatgcgga gcatgcgcca gcgtatgccc agtggaggct
    40081 cctcaacagg actaataaat taataaaagg gactgattaa agtgtgtatg tatacgcttt
    40141 aatcagtccc ttttatattt ccagaatcct tacagtttca tgtcattaat cgtatttagg
    40201 tattctttaa tttttgatgc aactgaattg atggttgatt cttcaaaggg gctttttaga
    40261 ttaccggctt ttaaaatctc aaggaaggat tgactcccgc cgattttaca gatttttaag
    40321 taatcttccc atgcttcctt tctgttctca ttaaacctta accaaaattg ataggagcaa
    40381 atttgtgcca gagtataatc gatgtaataa aagggagatg agaaaatatg accttgttta
    40441 aaccaaaaag tacctttatt taaaaattca ttatcatcat aatttttata aggggtatat
    40501 tttttttcga tatctctcca tgcttttttt ctttcatcag gagtcatatc aggatactca
    40561 taaacaatat gctgaaattc gtcaacggtc actccataag gtatgaagca caaagaatcc
    40621 gccaagtgga aaaatttaaa cttttctatg tctttttcaa aaaataattc catccaaggc
    40681 catgtaataa attccatgct cattgaatgt atttcggcag cttcagacgt agggaaactg
    40741 tattcgggcg catcaaaatg tctgctttgg tatgtttgaa aagcatggcc cgcttcatga
    40801 gtaacaacgg ttacatcgtc ttgagtgccg ttaaaattgg caaatatgaa aggacttttg
    40861 taatcgtgaa ggaatgtgca atatcctccc gcctgcttat ttggctttgt ttccaagtca
    40921 aataattctc tgtcaatcat tatatttata aattcatgag tttcgggaga caattcctca
    40981 tacattttct tggtgcgctc cattatccag tctttatttc cttttggagt cggatttcct
    41041 gatatgaaat tcaggggaat gtcatagtat ttaaattcat cgactcctat gcgctttgcc
    41101 tgtctttctc ttaattcatt cgtaatcggt actatgtatt tcaatacatg ttctctgaaa
    41161 tttcgaacat ctgttgaatt gtaatcagac cttccgtttc ttaaataagc aagctctata
    41221 aaattattaa atcctaattt taatgctatt tggtgtctaa tctttaccaa tttatcgtat
    41281 atcaagtcaa atttagcttc attttctttg aagaaattgc tgcttttttc atgagcttct
    41341 tttctgatac ggcggtcctt tgattccatg tagggttgca tttgagacaa gtttaataac
    41401 tttccgtcat actctatctt tgcggatgat atcagcttat catattcgct tattaactta
    41461 ttttcttcct gcattagagg aatcgcttca tcagaaaatg ttttaatctc catttccgct
    41521 aaggtaataa gctgaggatg tattttatct gttaattctt ccttaaatct tgaatttaac
    41581 agttgcttgt aaaattcaat cctcaatccg taaaatttcg gtgatgtcat atccatatag
    41641 tccttttcag tatcataaaa ttcatcagct gaattgatgg aatttcgtat atacaccagt
    41701 tgactcattg tgtcaacatt atttgctaaa gcattgattt tttttaggat ggtatattgt
    41761 tcatcggcag ttgctgagtt tttaaaatca ttaatcagat ttaaatactt ttcttttatg
    41821 atattataat caggtcttat atattcatac tcattgaatt tcataatgcg cctcgcttta
    41881 tatttagtta gctcttaact ttacatattt catatggcag aaaagataat aatattataa
    41941 caaaaattgt taaatatgca agacattagt gtttaaaaat aagattgcag ttgtttgtta
    42001 agatatggta taatgataaa accaaatcct tttggcacag actgaagaac ttatgttatc
    42061 aattgaaagg gaaattcgtg aaaaacatta ttgttaaagt attaaaaaac agcatagcgg
    42121 aagaattaaa tatagagccg ggagatgagc ttttaagcgt taataattcc ataataaagg
    42181 attacataga ttataaatat caaatttctg ataatttggt ttattttgaa atcaaaaaaa
    42241 aagacggaga aatttgggaa ctcgaaattg aaaaggatta cgatgaagat ataggaatag
    42301 tatttgaaaa tcccctgatg gataatatta aggtatgctc taataagtgt attttttgtt
    42361 ttatcgacca aatgccgaaa ggcatgagaa aaacacttta tgtgaaagac gatgatacca
    42421 ggctttcttt tctgtacggt aatttcataa ccctgaccaa tttatcggat gatgaatttg
    42481 atagaattat tcagtataaa ataagtccca taaaagtatc tgttcataca accgaccctc
    42541 aactaagaaa gtatatgatg ggcaatgata aagacatgga tattttaaaa tacttaaaaa
    42601 ggctgattga tgccaatata acggttgatt gccagatagt attagtaaga gacgttaatg
    42661 ataaagagca gcttaccaag accattaatg atttgtcttc tcttcacccc ggacttagga
    42721 gtgttgcagt tgttcccgtg gggctcacca agtacaggga aaagcttacg gagcttaagc
    42781 ctttcgacaa agaaagtgca aaagatgttg ttgagcaggt tacaaaactc caggaaacac
    42841 tgcttagaaa gttcggtacc agatttattt tcattgcgga tgaattttat attctaagta
    42901 aatatgattt tcctccctac gaagattatg aggattttga tcaattagaa aacgggatag
    42961 gtatgtgcag gttatttaaa aaccgtgtag aagaagagat aaaaaatata agatataata
    43021 aagtattaaa agatgaagtt acttttgtaa caggagttgc tgcttatgaa ttaatggtag
    43081 aattagccga aaaaattatg agcagaataa atgtgaaaat taatgttctt agaattataa
    43141 ataattattt tggagacaaa ataaccgtat caggtcttat tacgggaaaa gacatcatag
    43201 accaactcaa gggtaaaaat tataaaaacc ttatattgcc tagaaatatg attaatgaca
    43261 atcatgtgat gctggatgat atggtaattg aagatttgga aagagaatta aacactaagg
    43321 tagaaatata cgatgcagaa gatgatactt tgctcagctt aatcgttaaa taaacggagg
    43381 aaataaatgg gaagacctat agtaacaatt gttggcaggc cgaatgtagg aaagtcaaca
    43441 ctttttaata ggattgccgg aaagagaata tccataacgg aagatactcc cggtgttaca
    43501 agagacagaa tttatgctga atgcgaatgg cttgataatt actttacttt aatagacacc
    43561 ggaggaatag agccaaacac aacggatacg atttttgcac agatgcgcat ccaagcggaa
    43621 gtagccatag aaatggctga tgttgttttg tttatggtag acggcaaaga aggtgttacc
    43681 gctgctgaca ctgaggtggc acaaatgctc agaaaagcaa aaaaagaaat aattctcgta
    43741 gtcaataaaa tggacagatt cgtaaatgaa gacagcgtat atgaatttta caatttagga
    43801 cttggagacc ctattccgat atcatctgcc gaagccttgg gcataggtga tttgctggat
    43861 gaaattattt caaaattcaa tggaaaaatg gatacaaaag aagaggaaga cagcataaaa
    43921 attgctgtta tcggaaagcc taatgcaggc aaatcttcaa taatcaacag aataataggc
    43981 gaagagcgag taatcgtaag tgatattccg ggtacgacaa gggatgccat cgatacaaag
    44041 ttcgagagaa actccaagaa gtatacgctt attgatactg caggcataag gaaaaagagc
    44101 aaggttttgg aatcagtcga aaagtacagc gtattaagag cttttactgc agtggaaaga
    44161 gcagatatat gtgtattggt aattgatgcc gataaaggag taacagagca ggatataaga
    44221 atagccggat attctcatga cagcaacaaa ggaatggtta tcgtcataaa caagtgggat
    44281 ttaattgaaa aggataatag cacttttaag gaatttactg aagagataag acaaaaatta
    44341 gcatatgcca catatgcccc catattaaca gtttcagctc ttacaggtca gagggtcaac
    44401 aaaatacttg aagtagttga tgaaatatac agcttcagaa atttaagaat ttctacggga
    44461 gtattaaacg atattcttac agaagcagtt cttatgaatc aaccggcaac agtaaaaggg
    44521 aagagactta aaatatacta cggaacgcag gttgcagtaa atcctcctaa gtttttaata
    44581 tttgtaaatg ataaggaaat tgttcatttt tcatacgaaa gatatattga aaataaaatc
    44641 agggaagctt tcgaatacaa gggaacacca attattattg aatttagaaa cagaagcgag
    44701 aaataagatc caaactgtgt aatagataat aaggttatgc tggaggtatt atgaaatata
    44761 tttttatcgc aataatatct tattttatag gcaatatatc atttgcctac atattaggaa
    44821 aaatattaac caataaagac gtcagagaat ttggaagcgg caatgccgga acaacgaatg
    44881 caataagggc attcggcaaa aaagtaggcg cgatggtttt ttcaggagac gtattaaagg
    44941 gtgtcgttgc tgtcttgttt ggcagatatt ttgcgggaca aacaggagct tatattgcag
    45001 gagcttttgc aataataggt cacaattggc ctgttcttct taatttcaaa ggaggaaaag
    45061 gagtcgccac aactatcggt gttatggtat ttgtaaattt ttatctcact ttgatttgtg
    45121 ttattattgg cgtaataatt attattttta caagaacggt atccttgggc tctatttcag
    45181 gcatggcagt tgcaccactc gtggcaatta ttttgattcg tccgcttact tttgatcttt
    45241 tattatttac aatatttgtt gcaagtatgt ccatttacag acacaaggat aatataaaaa
    45301 gactgattaa aggaaatgaa aacaaatttt aggaggcgag gatgaataag aaaataacag
    45361 tcctaggcgg aggaagctgg ggaaccgcac tttccaaact tctttcggaa aacgggcata
    45421 atgttaccgt atggctgaga gatgaagctc aagctaaaga attaaccgta aaaagggtta
    45481 atgaaaaata cttgccaaaa gttaaaatac ctgaaaatat agtatttgct tctgatataa
    45541 acaaagctgt agaggaagcg gagattttat tgttggttat tccaacccaa atggttaggg
    45601 gtgtattaaa gcaaattaaa gatgagtata aagacggtaa aataattata aatgcttcca
    45661 agggtattga aaagggtacc atgaaattgg tttcaacaat tgttaaggaa gaaacaaaga
    45721 atacaatatt tgcggtttta tcaggacctt cccatgcaga ggaagtcggg ttgtcattgc
    45781 ctactgcaat aactgttgcc tgcgaaaata aagatgtagc cgaggagatt caagacatat
    45841 ttatgtctga atatttcaga gtatatacaa atgaagatgt attaggtgca gagttaggag
    45901 gagcactcaa aaatataata gctcttggtg cgggtatatc cgacggagta ggctacgggg
    45961 acaatgcaaa agcagccctt atgaacaggg gtattgttga aattgcaaga cttggacttg
    46021 ccatgggagc ggatttgcac acattttacg ggcttaccgg aattggagac ttgatagtta
    46081 catgtaccag caagcattca agaaactgga atgcaggcta ccttatcggt caaggcttaa
    46141 caaaggatga agcgataaaa aaggtaggaa tgattgttga aggaattcct accacatatg
    46201 ctgcatatga attgtctaag aaactaatgg tagaaatgcc gatagtcgat gcaatgtatg
    46261 atgttttgga aaacaaggcc gatgtaaaag atacagtaaa taaattaatg ttaagagaca
    46321 aaaaagaaga aagactttag tgacaagggg acgggggtat tgtcacaagt cttcagtcga
    46381 gtctcttgta atttaaaaga agaatataga gaatattaga atatattctt tttttgtttt
    46441 tttggaataa agaagttgaa caaggcatat aaattaattg tatacaatag tgcatatttg
    46501 caaggggagg gattattatc actaatatag atatatacga agatatagct aaaagaacca
    46561 agggtgaaat atacttgggg gttgtgggac cggtaagaac cggtaagtca acatttataa
    46621 gaaagttttc ggaaaatgtg atgctgccca atattgagga tgagtataca agagaaaggg
    46681 caaatgatga aatgcctcaa agcggtacag gcaagaccgt tatgactact gagccaaaat
    46741 ttgtaccgag tgatgcagta atggttacat tcagcaacaa tgtaagagca aaattaaggc
    46801 ttattgactg tgtagggtat atgattcccg gtgcaattgg aaatatggaa ggtgaagtac
    46861 caagacttgt tagtactccc tggaacgatg aacccatacc ctttgcagaa gcagcagaaa
    46921 taggtacaaa aaaggtaatc aacgaccatt caacaattgg ggtattagta acaaccgacg
    46981 gaagcatcac agacataaac agagataatt acataatggc agaagaaaga gtggtaaggg
    47041 aactgaaaga aattaataag cctttcgtta ttattttaaa tactactcat ccaaaaaatg
    47101 aaaatacagt tgctttatct tatcagttaa aggagaaata cgatgctccg gttcttatag
    47161 tcgactgtat gaatttaaaa accgatgata ttgaaaatgt gttagaaaat gttctttatg
    47221 aatttccgat taaagaaatc aatgttgaac ttccaagatg gtttgacggg ctatcatatg
    47281 attatccaat aaaaagaaat ttaattaaca tactaaaaga tgatttcgaa aattattaca
    47341 aattaagcga ctttaaagca gaggatattt tttcggaaga aaatgattat gttgaagatt
    47401 actatgtaaa agatattgaa cttggtacag gaaaaatcaa tctagcagtt gacctggata
    47461 attcttatta ttacaaaata atcagcgacc tttgcggtga agaaattaca ggtgagcatc
    47521 aaatattaaa gcttataggt gacttgtcaa aaacaaggaa gagctacagc cgtgttgagg
    47581 ctgccataaa cgatgctaag gctaacggct acggatatgt gacaccgact gtaagcgaca
    47641 tgattattga gcccccggaa gttttcaaag aaggcggcag gtacggaatt aaaattaaag
    47701 cacaagctcc atcattgcat atattcagtg ccggtgtcaa tacggaagta tcacctgtca
    47761 taggaagcaa aactcaaagc gaggatttaa tgtgttatct taccgaacaa atgaaagaaa
    47821 atccccaaag tatatggcag gctgaaatgt ttggcaaacc cctatcaagt cttgttgagg
    47881 aacagctgga ttctaagctt acggctatgc ccgacaatgc acgcaggaag cttagaaaga
    47941 caattgaaaa aatagtaaac gacggtcaag ggagcataat attcctcata gtataagatg
    48001 cctgacttaa gcagcgatat gtcctttata tcctaactta atttcttcga taaaagattt
    48061 tctatttcga aaaatgaaat atgaaaatct atttttttat tataataaaa atgtgttata
    48121 aattaatatt atattaatgt tgaaacgatc taataaacgt gcataatata ttattaatat
    48181 ttaaggattg gaaataaagg acaatcagta aaatatagat aaaaatctga ttgacaagcg
    48241 taaataagtg agctaaaata aaaaagtgat tgccaagaag ccgattgcat ttatgattta
    48301 aatttcagta gattttgtga aagttttttt gattaagatt gtaaagaaaa ctgagaagac
    48361 aagtcaaaaa tacagaaaaa atgagtgaaa aattattgac tttaattcgc agttatggta
    48421 aactaacaaa gctgtcacaa aaggcagagg cgtagaagac tggca
//
LOCUS       DAOSHP010000004        43438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_680_length_43438_cov_8.936703, whole genome shotgun sequence.
ACCESSION   DAOSHP010000004 DAOSHP010000000
VERSION     DAOSHP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 43438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43438
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_43438_cov_8.936703"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..884
                     /gene="thyA"
                     /locus_tag="PLB33_00915"
     CDS             57..884
                     /gene="thyA"
                     /locus_tag="PLB33_00915"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOG62110.1"
                     /translation="MSTADKIFKEMCKDILTKGVWDTDYDVRPRWEDGTPAHTIKKFG
                     IVNRYNLQESFPILTLRKTNFRAAVDELLWIWQKKSNNVNDLNSRIWDSWADETGSIG
                     KAYGYQLGIKHKYKEGEFDQVDRVLYDLKNNPASRRILTNIYNFQDLHEMHLYPCAYS
                     MTFNVSGNKLNAILNQRSQDVLAANNWNVVQYSILVHMMAQVSGLVAGELVHVIADAH
                     IYDKHVPIIEELIEMEEHEPPTLEIDNIDDFYKFTVDSFRLINYQYNPFKVKVPIAV"
     gene            895..1416
                     /locus_tag="PLB33_00920"
     CDS             895..1416
                     /locus_tag="PLB33_00920"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOG62111.1"
                     /translation="MKLIVAVARDWGIGNKGELLFNVPEDMEFFKKTTLNKVVVMGRK
                     TLLSLPGGTPLKNRTNIVMTSDVNFKKEGCIIVNSKEALFEELKKYESDDVFLIGGGI
                     LYKELYPYCSEAYITKFDAILEADTYLHNFDEDKDWLLTYASEIHEHNGVKFIFTIYK
                     NLTLNDKGTGVLS"
     gene            complement(1569..3044)
                     /locus_tag="PLB33_00925"
     CDS             complement(1569..3044)
                     /locus_tag="PLB33_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62112.1"
                     /translation="MSEKDYNFDKQIIEQLSYVTPPEEEIRKTNPWSKPIGFITWGFI
                     LTTFKLNIIYLQYILPVIGVIHIFLGFRSLRKENKYFKIAWLLSIIKLSLHLIDLAWI
                     STPLNIMDYPNLIIGSVMLPFQISMYLVFRAALKETSKKAGRLMETDPLLWASLWTMA
                     AYLIALSPLSNSWLIFIPMVVSYIFIVRSLYHIGDEMDDTGYVLTNAPIKISNKIFGW
                     TYFLIAFAVVITCSVFSNHLKLQSQEYLHPETTETRQHLLDMGFPAEALRYLNNEDLM
                     MLSDAVNVESFSKLLMFDAKRIEHEERYGNYIQITHTYEPGKKNMDSTTVYIELPKNV
                     VYVMQYFKWKGGRPIWQDGILISGETAADDKQIISSGLFYSKKGTEYTADFPRLICDR
                     MTQHTMFGTYYPVLITGTLSYPFGSEEQGGYVLYRYKVMTDSDIYDTYNIFSYVHLSS
                     PLHMPYTRTEDLILSGGYTFEDMLQQHQTNYESMALREKIK"
     gene            complement(3041..3568)
                     /locus_tag="PLB33_00930"
     CDS             complement(3041..3568)
                     /locus_tag="PLB33_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG62113.1"
                     /translation="MDEKIIELVKSSKDKGLTLMQEQYSGLMHYIVRGILQNESDIEE
                     CISDICIKVWNSIESYSPEKGKFSTWLTVISRNTALNYMKSKYTEHDELKEETADKHS
                     VEDDVLRRERAEELKCAIRTLSPEEQHIFYRKYYYLQRTSQIAAELGMTERSVEGRLY
                     RLRKKLQKKLGGDFI"
     gene            complement(3737..4087)
                     /locus_tag="PLB33_00935"
     CDS             complement(3737..4087)
                     /locus_tag="PLB33_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOG62114.1"
                     /translation="MTNPSIYKMSVAKVYPFYVAKAEKKGRTKAEVDEIIRWLTGYSQ
                     EEFQEQLNKQTDFETFFLEAPLPNPARTLIKGVVCGVRVENVEEPIMREIRYLDKLID
                     ELAKGKAMDKILRK"
     gene            complement(4161..6806)
                     /gene="ppdK"
                     /locus_tag="PLB33_00940"
     CDS             complement(4161..6806)
                     /gene="ppdK"
                     /locus_tag="PLB33_00940"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOG62115.1"
                     /translation="MSKKWVYLFKEGSASQKSLLGGKGSNLAEMTNIGMPVPQGFIVT
                     TEACTEFYNQGEKLSEEIISQIRTNLSILEKQTEKVFGDTNNPLLVSVRSGAKFSMPG
                     MMDTILNLGLNEDTVEGIARKTGNTRFAYDSYRRFIQMFSDVVLGVPKSLFENALEAK
                     KEKNGYKFDTELTADDLKELVQEFKQIYKNEVGEDFPDNPEKQLLMAVEAVFKSWNNQ
                     RAITYRNLYNIPHDIGTAVNVQSMVYGNMGDTSGTGVAFTRNPSTGEDKVFGEFLINA
                     QGEDVVAGIRTPLTIDKLKDIMPEIYDEFINICEKLEQHYKDMQDIEFTIEEGKLYIL
                     QTRSGKRTADAALRAAVEMVDEGLISEEEAVLRVEPKSLDQLLHPKFDPVELKKSSPI
                     AKGLPASPGAASGKIYFTAEDAVEASLKGEDAILVRKETSPEDIEGMSKAKGILTSRG
                     GMTSHAAVVARGMGKCCVAGCESVSVDEKNKTMTVNKAVYHEGDYISLDGSTGSVYAG
                     IIKTVPANISGNFEKLMKWADEFRRLKVRTNADTPHDSEAAVKFGAQGIGLCRTEHMF
                     FEESRIFSVRKMIISDTVEQREKALAEIMPMQKNDFKGIFKAMGVRPVTIRLLDPPLH
                     EFLPTEEDDLIELAEAMNVSLQEVKAKVNSLHEFNPMLGHRGCRLAISYPEIAKMQTR
                     AIIEAAIEVSREENLEIIPEIMVPLVGKKEELLILRKLIIETAEETKKEFNSDLNYLV
                     GTMIEIPRACLTADEVAEYADFFSFGTNDLTQMTFGYSRDDAGKFLEDYKEARILEQD
                     PFETIDQEGVGILVDMAVKLGRKAKEHLHLGVCGEHGGDPASVEFFHRVGLDYVSCSP
                     FRVPVARLAAAQAALKDKMNVHIDK"
     gene            7047..8480
                     /gene="glnA"
                     /locus_tag="PLB33_00945"
     CDS             7047..8480
                     /gene="glnA"
                     /locus_tag="PLB33_00945"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOG62116.1"
                     /translation="MFSTVNEVQEYCVSNKIKMIDFKMIDINGRWRHLTIPAERLNED
                     TMKYGIGFDGSNYGYATVEKSDMIFIPDLTSAHIDPFVDIPTLSMIGNVFVLHPDGNK
                     PFNQYPRNVALRAEEYLRESKIADSMIIGPEFEFHVLDHISYECKPNKVAFEMDTSQA
                     EWNSGNNESRNLGYTVPLKSGYHITAPHDITYGLRSRMCLLLEDWGVEVKYHHHEVGG
                     PGQVEIEVELGELTEMADKTMIIKYVVKNSAIAENKTATFMPKPFFAEAGNGMHVHML
                     LMKDGKPVFYDENGYSGLSREAHWFIGGLLKHVRSLCGLTNPSTNSYRRLVPGYEAPV
                     TIGYATSNRSAVVRIPAYCKSPEAKRFEIRNPDGTCNPYYAYAAILMAGIDGIINKID
                     PHENGWGPYDFNLYNLSPEEQKKLSALPQSLDEALKALEEDHDYLLRGGVFPEELLNT
                     WIANKRMEMNEINKIPHPAEFAYYYDL"
     gene            8763..10664
                     /gene="dxs"
                     /locus_tag="PLB33_00950"
     CDS             8763..10664
                     /gene="dxs"
                     /locus_tag="PLB33_00950"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOG62117.1"
                     /translation="MKLLDSINSPEDIKHLNMDELNSLCRELREYILENVSKTGGHLA
                     SNLGVVELTVALHRVFNTPHDKIIWDVGHQTYAHKILTGRKNKMSSLRQLNGISGFPK
                     RNESIFDVFDTGHSSTSVSAAMGMARARDLNNEDYEIVAVIGDGALTGGMAFEAINDA
                     GRSNTKLLIILNDNEMSISKNVGGLSRYLSRIRTTKGYLSTKRDVEKILNKIPVGKQI
                     KSFLKRAKDGIKQIVVPGMLFEELGLTYMGPIDGHNIHKIIESLERAKNIEGPLLMHV
                     ITKKGKGYKFAEERPNEFHGVSAFNIETGEALKKSSQSYSDYFGMKMCEIAEKNENVV
                     AITAAMTDGTGLRDFAKKFPNRIYDAGIAEQHAVTMAAGLAVSGVVPVVALYSSFLQR
                     AYDQIIHDVALQNLHVVFAVDRAGLVGNDGETHQGVFDEGFLSLIPNMTVLSPADYRE
                     FGNMMEFAVSYNGPVAIRYPRGSMSEYIKNSEQSIVLGKGYIVEEGKDITIAAAGKMV
                     KTAIETREILKTKNIDAEVLNLRFIKPFDKELIINSVNKTKSLVIIDEATVFASYAVN
                     IYNILPKNLDVMVKTLPDEFIKQGSIEELLKENQLDADSIAAEIIKWKADNYDIDISN
                     NYTCLKQGR"
     gene            10789..11868
                     /locus_tag="PLB33_00955"
     CDS             10789..11868
                     /locus_tag="PLB33_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62118.1"
                     /translation="MNKVEIVVGDKKYSVRTDESPEYVRKIESVLNDQINIIANQNKR
                     FNEIDKLILSSFVVIDRYLKLSDEINEYKKDICEEILSLKEAKELSEKEKEEAVNKAA
                     DAIIEKERFKEKLMARDNDREYLNSQITKLQEKVSEQEQQLLKSEMLINELKIKNEEL
                     IEVNEELIKERENFTKEISFMNNTKASLNGRISKLQLKLNEKEQEIIKLEKTIRDLKS
                     AVDDKSQKIYNFSDEQQKMNLLVDSKEKDINSLINKINLLQNKLNEKDESIASKDKLI
                     NDLKSTEEVLKQKYESINDEKEKYLEELLMINSDKESLINSINQLQEKLNRKEAENFQ
                     NQLEINKLKKDNMELMQLLDEETAK"
     gene            11904..14075
                     /locus_tag="PLB33_00960"
     CDS             11904..14075
                     /locus_tag="PLB33_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOG62119.1"
                     /translation="MSVELLAPAGSIESFYAAVNSGADSVYLGGKFFNARYNSRNFDN
                     DEMKHIVKYAHNKGVKVYVTMNTVLKDNEITDALNYAIFLYENDVDALIVQDLGLFCL
                     VNKYLPDFPVNISTQASVYDEYGVKFFEEFNVKKVIMARELSLTELKETVKNTDADLE
                     VFIHGALCTCYSGQCYMSSFLGGRSGNRGKCAQPCRLNYSFYDKETKEEIESFKEVPL
                     LSLKDFIAGETIYELIEAGIKTFKIEGRMKGPAYTSSVVEYYRQIIDNYYSGKNIDST
                     HLKEKAMETFSRGYTNGYLKPSKDDEMFAKLSSGIKGENIDEIEEDVKDKAQEYSRFR
                     RNKISFDIELVIGEYALLKVFDGKNTVEVKSKEVCESSIKNNVTDEIIYEQLSKLGNT
                     IYELHDLKIVKDEGVFIRKSSLNQLRRDATEILYEKCSKFYDRRKVNTLTYNDIFSNI
                     NKESHSAKISLKINSIDELKYADDVKVKRLYVPFYLNIEEVKNTKVVEKYLWVPNIVS
                     KENYKILKENLNLYDEIFDGVCVNNVGSFYFFKKNSRLKLHCGPFFNIINTFSAELLK
                     EKGAHSFTFSVETNIRDMESIVKHTSLSSEIVAHEYVQLMVMKNCPMSVIKNCKNLQD
                     CEKCSFKSKYQLKDRKGIHFNIERQNKLTHIYNSVPLSIIGIANNFVEKNIDYFLVDT
                     KFLEDAEEIIDTLYCEIKGVKTHYILEENKFTRGHYLKNVL"
     gene            14290..16665
                     /locus_tag="PLB33_00965"
     CDS             14290..16665
                     /locus_tag="PLB33_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOG62120.1"
                     /translation="MKEKSKSVLEFDKIIEKVAGFAETKMGKEEVYKLDTSHNLEGVK
                     FKQEQTAQALSIIIEKGSPPLGGISDIKEYVKRGAVGGIISLRGLLNCADTLRAARLL
                     KNYVLLNNDDRNYDLLEDLCNSIYVNKDIEERIYSVIISEEEIADDASPELKRIRREI
                     QIKNNSIKNKINSIVSSSSMQKYLQEAIVTMRNDRYVIPVKKEYRSMVKGIIHDQSAT
                     GSTLFIEPMALVEMTNDIANLKIEEKKEIERILLELSMMIGDISEEMLNNQEILKELD
                     FIFAKGKYAISINGIEPKINDRGYIRIKKGRHPLISPELVVPIDAVLGDEYTTLIITG
                     PNTGGKTVSLKTLGLFTLMGMAGLHLPAEYGTEISVFSAVYADIGDEQSIEQSLSTFS
                     SHMTNIVKILKEVDEKSFVLFDELGAGTDPTEGAALAIAILDTLHERKILTAATTHYS
                     ELKLYALTTEGVINGSVEFDVETLSPTYKLLIGIPGKSNAFEISRKLGLQQEIIDKAK
                     KNIETEKIEFEDALKQIEENRIYIEEKKQEIDRLESESKKKHSDFLMKERKSLEKSEK
                     IIDEANYEARKIVENAKREAAEIIKELRKLNLEMDKDKNRRVNELRQALNEKTKELSE
                     NIYSDIIEEEDTIDTDTPLKIGDAVTVRSLNQKGFIISDVDDSQTVMVQIGLIKTKVK
                     KSDLIKIKSDEEVKQKTNASRKVRLRTSSINPTIDIRGYNLDDALLELDKYLDDAFMS
                     NLNEVQVIHGKGTGVLREGVRQFLKNHKHVKESRLGTFNEGGDGVTIVKFK"
     gene            16729..17811
                     /locus_tag="PLB33_00970"
     CDS             16729..17811
                     /locus_tag="PLB33_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62121.1"
                     /translation="MNVRDKYSNKAVIKYTGAFIAWVIGSGFATGQEALQFFSSYGYL
                     SYGVLLVNLIGFLFLGQVLLVTGFEHKTESSFNHFKYFCGEKIGTIYSWLIPITLILI
                     MPVLISGAGATLSEYYGVNRYVGSFLMSVLVLSVYLIGFEHLIKIVSAIGPAIIIFSL
                     LVGAITLANDYGNFSDVPKYQFKLNELQTAPHWTISAILYLSLNFLSGSVYFTELGKS
                     AYNRNDVKYGAFFGALAIILNIAIMNTAILLNAENISTLAIPVLYLAKKISYMLGAIF
                     SIILILGMFSSCSAMMWSICNRFTRGGKRGNRIFAVFISILTFVLGLFSFSELIGTFY
                     PLVGYVGLFFIGSVIYKGIKYNIIKN"
     gene            17822..18043
                     /locus_tag="PLB33_00975"
     CDS             17822..18043
                     /locus_tag="PLB33_00975"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HOG62122.1"
                     /translation="MERIIKDVNCPKCGQVMEEGYIHSRKQILWSEDDKPRLFDFYDE
                     NLVTMPIRRANKVPAWRCKECKIAIFRYE"
     gene            18165..18944
                     /locus_tag="PLB33_00980"
     CDS             18165..18944
                     /locus_tag="PLB33_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOG62123.1"
                     /translation="MRQYVVDAFAEKVFEGNPAAVCILDEWLPDEVMLNIARENNLAD
                     TAFVVKDGTNFKLRWVTPGGEIDLCGHATLATGYVISRFIAPELSEVVFQTKSSGILK
                     VLKKGELYEMDFPAYKLKKVEVTNAMADAIGVKPIEAYMGRDLLCVLESEDEVRNLNP
                     NLEKVKLLDGLLFHVTAKGNEFDCVSRSFVPKLSGTEDPVCGSGHCHIIPYWAERLGK
                     DNLIAYQASQRGGTLYCKYQGDRVIMGGKVALFSTADINIE"
     gene            19015..20094
                     /locus_tag="PLB33_00985"
     CDS             19015..20094
                     /locus_tag="PLB33_00985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62124.1"
                     /translation="MEREIVRLDMDRDFEAFEDLLKISFGEATNSKKEMYEWLFENNP
                     YNKSGNMMYLLKEGDKVIGCDGLLPNELYVNGKTLLTAHSVKSMTHPDYKRQGIFRKM
                     TENSCERGKQDGVDVIIGLANDQSYPAYQKFGWPTLFEKEVYVKPILINNILKRRIKI
                     GFLSEAANSVYTVFMKNKLKNQMDKEIKFEILDRVPEEIQKCWDRYKSKYNVLLVRDY
                     KYLNYRYNERPDINYVTILARHKGEIIGFAILHNAVANGSNMTSAVEFFTDPDNERYI
                     KALANGIAKYCYDKGLEYAVIGTGHYGEYRKVLLNNGFMVTRKPPKNNMMIAHILSDK
                     VTLEEITGHEKWHITQGDGETELDL"
     gene            20383..21252
                     /locus_tag="PLB33_00990"
     CDS             20383..21252
                     /locus_tag="PLB33_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG62125.1"
                     /translation="MIKLIVDSTCDLNCEILDNHDVEVIPLSITIDDKTYLDGVDITT
                     DRVYEYMKDGKVPKTSQVSFESVSQAVEKCISKESDFIYLAFSSKLSGTYSFAKKIID
                     MYKEKYPERKMAIIDSKGGAGGTGLIAIQVLKMIERNLPFEYILNQAQFMVQHIVYRF
                     TISDLEWLSKGGRISKSAGYVGNVLNIKPYLVLDDGLIKVSKMIRGSRKTIQTLIQDI
                     EDGTDKFKNQIIGISHADDLDTALMVEQKIKDSIKGCKTTIFKIGAVLGVHLGIGGVG
                     VFFFDEKPEFYEL"
     gene            21405..23111
                     /gene="argS"
                     /locus_tag="PLB33_00995"
     CDS             21405..23111
                     /gene="argS"
                     /locus_tag="PLB33_00995"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOG62126.1"
                     /translation="MDFKEKVAEIFTSIETELSMDDALQLIEIPPSSDMGDYAVPCFK
                     FAKKYRKSPAAIANEIVEKIGAVEEFEKIESAGPYVNFFVNKSQFAKKILKQAFEEKE
                     NYGRTNVGEGKTVIVEFSSPNIAKPFHIGHIRSTLIGNAINNIYKYLGYNTVAINHLG
                     DYGTQFGMLISAYKKWATPEIIEAIEKDPIPELLKLYVRYNQEIETHPEYQEEARYWF
                     NQLEKGNQEAYQLWAWFREVSLKEFNRVYDMFGIKFDSYAGESFYSDKMPAIIDELED
                     KGLLKDSEGAKIVELSEFGLTDALIQKSDGATLYVTRDVAAAKYRKETYNFYKNIYVV
                     GAQQKLHFDQWRKIIDLMGYDWAYDCVHVMFGTVSLEGGAIATRKGRVVFLEDVLNKA
                     IEKTTEIINERNPGLEDREKVAKQVGIGAVIFQELFNNRIKDYVFSWDKTLSFEGETG
                     PYVQYTFARTCSLLRKADIDINDDVDYSVLTDSESFNVIKKLEGFKEAVLNAHDKYEP
                     FMITRYIVGLAQEFNRFYHECQIITDDESVKKARLLLTLTVNTVLKQGMKLLGMEAPE
                     KM"
     gene            23272..23919
                     /locus_tag="PLB33_01000"
     CDS             23272..23919
                     /locus_tag="PLB33_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOG62127.1"
                     /translation="MKRVNYLTFLIFLCIFSYFMMINTSMTAIAEDNQNYYKFSQDKI
                     IKSIIHDYRDSFIREKELIRYTEELTGLDYEHCRFLIKESKEKYIDPFIVLGLIKRES
                     DFNPKAKGVGGELGLGQLMENTAQIIAMNLGYSYNNEMLLDPVFNLKLTIAQLSYLNK
                     VYDNDMHKTLTAYNRGQQGLKNYLTTCDGRGVSDYSTKVLQFASIYKEEFENLKY"
     gene            24098..24952
                     /locus_tag="PLB33_01005"
     CDS             24098..24952
                     /locus_tag="PLB33_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62128.1"
                     /translation="MQYKINKMLNFCFDDIQKIDFQLFLITVKCLIPYDDKIGCLFDK
                     DRYKKEIELFSSYMNGNDETIDYWLRNKEASNLDDNLLEYKIIPLALSNTVWENLTDE
                     LIKVVLFYTINKNAILNALLISSAVFDFMTGSDIDSIEAAARERLINFSVKEFLIKNQ
                     INADKNYIIDFEKERIKMIAKPILFDNNFINGFKVLNYIFEKSPEHLTENNSDIIDSF
                     SVYLYKLRKGLISPEKLIIPDKIPEIKECLKYSTFSHPLLGRCKVIKRTEKEAIVRNK
                     LGLMRIRI"
     gene            24949..26661
                     /locus_tag="PLB33_01010"
     CDS             24949..26661
                     /locus_tag="PLB33_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOG62129.1"
                     /translation="MKILLVTLDSKFIHANLAVRYLKKYCRGFDIVIKEFTINQKIEY
                     ILDEILAEDADLICFSCYIWNIDYIKDISYIIKESNNKAKILYGGPEASFEIKELMEN
                     NPFIDYVIFGEGEETFKEFLEEIQKTNPNLHKIRGLAYKENNDVIINEGREPIDNLDI
                     INYPYEVDDKFANKIIYYESSRGCPFSCSFCMSSIDKKMRFFSMDRVKKDLKMLLNTK
                     ARQIKFVDRTFNADFRRSMEIMQYIVENNRNNMTIHFEITADIINDEFLSFIAKMPVN
                     MFQFEIGVQSLNEDTLREINRHMDKDKLCRVIKEIGKNKNVHMHLDLIAGLPYEDYES
                     FKRSFDGIYNLDAEKIQLGFLKVLKGTKIYEDREKHQIKYGLKAPYEVICTKYISLKE
                     LLKLKNIEELLDKYYNEKYFSKSLKYITCYIFPDSAFEFYSSFSDYWQKYNLYNYAHS
                     RKELYKILYDYIVHMKRKNDEFVQALKFDYIFNNQYEELPDYLNRETEDKFKSIKRQL
                     ANDIEFKNKYFPSMKGKIINDFRIAEIGGKIVLFVYRDRENIFNRCDTYVVNNIEEIV
                     YGKR"
     gene            26648..27775
                     /locus_tag="PLB33_01015"
     CDS             26648..27775
                     /locus_tag="PLB33_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOG62130.1"
                     /translation="MERDKYFEEYYEKKRNNISFITLKKGAAVNYKDKTYAAKKELPV
                     PVRIEKLLEDINKQNEMDGITLNNIIDGIIYILGTDRNFEYIQDYKEMFKELNFELIP
                     YIIYCINNDIKTEDGVVYGRALINSEENEKTCFIYASCLEKMGMEYHEKGNDSSRYFL
                     EEAHFYYEKCLDYNEKFSLAYYKLGYYYKRKQQYVKAELIWQKHQELDDNDLRIEEIR
                     NELIQLKPFVDYEKGYNLVLKERPDEALDLLLPLVKDFGGWWNLLFFIGLAYRSKREY
                     DIAETYFENVLKIDSTQKETLNELGLCKICTGKYVEAAELFSKLLSLDPGNCEVFCNR
                     AVAYLYNNQLERAKEDIQTALKINPEDAVALGIKEELEKKY"
     gene            28009..29235
                     /locus_tag="PLB33_01020"
     CDS             28009..29235
                     /locus_tag="PLB33_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOG62131.1"
                     /translation="MARKNFGLKNFFLSFALVVIAIYVFQNIKSPADFMIAENGILED
                     VIKAEGIVVKDEKVYSATLDGNVLYYYEEGTKIKQGQLIADINTDADSVEVNRQIAEI
                     QAAINKLENSSQVLTKENFTAYQNELQISILNNDLQNMYGIVEQMTNNDFYGSKYEDF
                     SISQLIEMKNSLANSISTHKVPYYSDRAGIISYKTDGLEGIYRFENVMDMTPSSTLRR
                     EYSLKDKRESVQKDEAFYKIIRNFEYYIIVTINNEKARLFEENKYIKVRILSDGEQHI
                     VWGYIEKINYGSEESVLILYFDDYFYKVYDKRYVNIELITAAYEGLKIKNESLIEVNG
                     IKGVYVQDASNIIKFFPVQILGSNEEYSVVFPGDYIGINERNIITIGEKRYPTIKIFD
                     KVILEPDKVYEGQIAD"
     gene            29245..29949
                     /locus_tag="PLB33_01025"
     CDS             29245..29949
                     /locus_tag="PLB33_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOG62132.1"
                     /translation="MSIRENIDDIVKRIEDTCKKVGRNPNDITVIAVSKTVESERARE
                     AFEAGINNLGENRVQELVKKYDELKDLDIKWHMIGHLQKNKVKYIIDKTVLIHSVESL
                     SLAEEINKRAEKNNLIANVLVELNIGEEDSKFGIKEESVYDFILSLEKFENLRVVGLM
                     TVAPFCENPEDVRWVFKKMKYIYDKISTMNLRNTKMKYLSMGMTNDFEIAIEEGSNII
                     RIGTAIFGARKYKEEE"
     gene            29950..30387
                     /locus_tag="PLB33_01030"
     CDS             29950..30387
                     /locus_tag="PLB33_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOG62133.1"
                     /translation="MSMMEKVKDFIGITDLEEDYEEEDLVSEGTERNKNERMGTNTKR
                     NNVIKVHSNTDMKVFICEPEKYEDCTKAVDEIKNRKVVVLNIEGMELEDQKQVFEFIK
                     GAIYALEASIQKVSNGIFVLAPNNVQIDGRLSDRYERNYYYGK"
     gene            30397..30660
                     /locus_tag="PLB33_01035"
     CDS             30397..30660
                     /locus_tag="PLB33_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOG62134.1"
                     /translation="MFYIIQLALASLLRIIYYLILVRVILSFFPTLQTSRISYFIYQM
                     TEPVLAPCRAILDKLGLGMGMFDFSPILALLLLNILQRLIYAI"
     gene            30687..31469
                     /locus_tag="PLB33_01040"
     CDS             30687..31469
                     /locus_tag="PLB33_01040"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HOG62135.1"
                     /translation="MKSLEKYYDFIRDDEIKNTVKKIQDKASYVNKNYIAAITEFINP
                     YVVKISIPVIKSYDIKFEIFPSYENCERKVFILYPQYSEIDENEFISGIRITNKSKFK
                     KLSHKDYLGVLMSLGIERSKLGDIYVCDDYGDVVVHSDIADYIIYNLDKIGHNKIEAE
                     KINLSQVNFKEQEHEILNIITSSMRIDNIVKHLTKLSREKASFIIKSGDVKINHIPEY
                     KVSTELKEEDILSIRRAGRFKIYKIIGTTKSGKLKIQIKHYI"
     gene            31481..31945
                     /gene="lspA"
                     /locus_tag="PLB33_01045"
     CDS             31481..31945
                     /gene="lspA"
                     /locus_tag="PLB33_01045"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOG62136.1"
                     /translation="MSIIAVFIIIFSVVFDQLSKYWATEVLKNGESIKIIGNFLRFTY
                     AENRGAAFSILQNQRVFFLIITIIMLIILGIVYFTNRNLSKLSRLSMAMIAGGAIGNF
                     IDRYRMGYVIDFIDVRFGTFYNFPVFNIADSFVVCGTILMVILILFNKFETS"
     gene            32061..32978
                     /locus_tag="PLB33_01050"
     CDS             32061..32978
                     /locus_tag="PLB33_01050"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG62137.1"
                     /translation="MEISDSEKEIILVSDEENKRIDVFISEKSGESRNFVQKLISDGN
                     ITVNEREVKANYKVKINDTIKIIIPPPEILDVKAENIDLDIVYEDNDLAVINKPQGMV
                     VHPAPGHYSGTMVNGLMYHLKNLSTINGVLRPGIVHRLDKNTSGLILVAKNDRSHNFL
                     AKCLKEHTINRIYYALVKGNIKKDEGEINAPLGRSEKDRKKYTVTNKNSKEAITNYWV
                     VERFGKYTLLKLKLKTGRTHQIRVHMKHIGHPVVGDDVYGNVNEFGLEGQLLHSKILG
                     FIHPTTGKYMEFESELPDYFVKVLNKIKS"
     gene            33035..33565
                     /gene="pyrR"
                     /locus_tag="PLB33_01055"
     CDS             33035..33565
                     /gene="pyrR"
                     /locus_tag="PLB33_01055"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOG62138.1"
                     /translation="MKIKALIMDEKAIERATFRIAHEIIERNKGVENIVLIGIKTRGW
                     PLSIRLAKKIEEIEGSKVPVFPLDITYYRDDVEKDDRAPLSVKSFDFDIKNKTVVLVD
                     DVLYTGRTVRAALDAIVDQGRPNNVELAVLIDRGHRELPIRADFIGKNVPTSRSERIS
                     VRLEETDKINQVVITE"
     gene            complement(33610..35370)
                     /locus_tag="PLB33_01060"
     CDS             complement(33610..35370)
                     /locus_tag="PLB33_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOG62139.1"
                     /translation="MSLDGIFLNSIINDLYKKLIGGRVDKIYQPDKNEIVISIRSKGE
                     NYKLLITAISNSPRLHLTNVIRQNPSEPPMFCMLLRKHLTSAHITNIKQINFDRIVEI
                     SFECKDEFGTAVNKSLITEIMGKHSNIIFINEDKTIIDAIKRVAENISSVRQVYPGLK
                     YVIPPGSDKLNPLTVTKDLFMKEIESTNNGIYIYNFLYKNFLGLSPFISREICYLTNL
                     NESTYLGELSEEKKEEIWNVFSKIMNKIKQNDFSFNIFYDENMNQYGFYCLEVEYLKD
                     YERKNFLSPGELLDEYYYELDNKNKINQRVASLIKNINTKIDRNRKKVEKQRNELLNA
                     ENRDKYKIYGELILANLHKTPENHKLRVINYYDPEQKEITIPLDPRFNLPQNSQKFFK
                     RYNKLKKAEEELEKLIDITLNEIKYLENILFSIEECETIDDLNDIYSELISEGFMKRK
                     SKADKSNKTKPITAFISSGGHEILVGKNNTQNDMITFKIAKNEDYWFHAKNMPGSHVI
                     IRTKGDELTDEEFIEAAKIAAYYSKGKNSSTVEVDYTKKSNIKKPPNAKPGFVIYDTN
                     YSMLVKPNIEGIKRDSGDVS"
     gene            35667..36554
                     /locus_tag="PLB33_01065"
     CDS             35667..36554
                     /locus_tag="PLB33_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOG62140.1"
                     /translation="MANIFSMTGYGKGEHNDGKRSITAEIKAINNRYCDINIKTPRHL
                     RIYEDNIRKILKNGIQRGRIDVYINIEYISESDTVILPNLALAKQYMDAIEKIKSELG
                     INGNPSLDTIIKFQDVLIAKEDDADDDELRICLENAMNEAVKNLLTMRVKEGIQLKDD
                     ISLSLVKIRELMEVISENSHTIVEDYKIKFEDRIKELLGNTHELDENRLYNEIVIYAD
                     KSDINEEIVRFNSHMVQLSDALDSGGAIGRKLDFILQEANREINTIGSKVSSLDIIQV
                     VIEIKNLLEKIREQVQNIE"
     gene            36564..36812
                     /locus_tag="PLB33_01070"
     CDS             36564..36812
                     /locus_tag="PLB33_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOG62141.1"
                     /translation="MSNTINIGFGNIALANRIVAIVSPESAPIKRLIQETREKNKLID
                     ATYGRKTRAVIITDCDYIILSAIQPDTMALRFENHNIK"
     gene            36822..37451
                     /gene="gmk"
                     /locus_tag="PLB33_01075"
     CDS             36822..37451
                     /gene="gmk"
                     /locus_tag="PLB33_01075"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOG62142.1"
                     /translation="MDEGLLVVISGPSGAGKGTICAQLLLEMSYLKVSVSVTTRKPRA
                     GEVDGISYYFIEEEEFINRINNDEFLEYAKVYGNYYGTPKEEVFKQLKAGNDIILEID
                     IQGALQVKKNYPMGVFIFILPPSLEELKSRIEGRGTDSKEVILRRMESAYEELNYAFQ
                     YDYVVVNDHVDTATEKIKHIILAEKNRVIRKKEIISKIREIKNEKNINR"
     gene            37429..37716
                     /gene="rpoZ"
                     /locus_tag="PLB33_01080"
     CDS             37429..37716
                     /gene="rpoZ"
                     /locus_tag="PLB33_01080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOG62143.1"
                     /translation="MKKISIDKLNDMVDSQYSLVTIISKRARQIIDGSEPLIKTKTKK
                     PVSIAIEEFYERKFEAIYNYDKYKKSLTANKETAVETTDEKKDMDETTAQE"
     gene            37770..39974
                     /gene="priA"
                     /locus_tag="PLB33_01085"
     CDS             37770..39974
                     /gene="priA"
                     /locus_tag="PLB33_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOG62144.1"
                     /translation="MYNTFVQVILNNISKSTDKIYTYGVPDALKSFVDEGKRVRINFG
                     RGKNIIDALIVSVDLTCDYPIEKIKPILDIIDEKPIVTKEMIKILFWIRERYLCKYSD
                     AMRLMIPSETKSIYEKHIRLKEDKEISQYKINKTENQIKVIEYLKNHKSASIAQLMDR
                     LNISKAVINNLIKRDIIEESLVFDYSNQREFQSDEPLCLNEEQKNACDTILNSDKKVF
                     LLHGVTGSGKTEVYMSIIEKYLKEGRQSIMLVPEISLTAQTIERFEKRFGNKIAVFHS
                     KLSKKDRYIQWMRVYKKEADIVIGARSALFAPLNRLGVIVIDEEHEDSYISTTSPKYD
                     TMEVAVKMAYMMDGKVILGSATPSLNTYYMAMKGQIELIEMKNRVKDAEMPEMKIIDM
                     SAELDKGNNTIVSEDLYESIKNSLKQNEQVILFLNKRGYSGFVSCKKCGYVVRCQRCD
                     VSMTYHVVGNIMKCHYCGSAKKMIYTCPKCGSEKIEQFNAGTQHVEKLINQLFPYAST
                     ERMDLDSMTDTESYENIYNDFKNKKIDILIGTQMLAKGFDFPNVTTVGVLSADAILNL
                     PFYNASEKTFQLITQVSGRAGRGDKRGNVYIQTYEPENFIINAAKNNDYELFLHEELK
                     LRKEFAFPPFINIINICLISKIEELVVKTANEKYLEVKETVKEMVKERSLLLYRPVPH
                     NIYKVNDEYRINLYIKVSNHSLSELKKILRTVYMEKDIENIKVSININTDTI"
     gene            39987..40445
                     /gene="def"
                     /locus_tag="PLB33_01090"
     CDS             39987..40445
                     /gene="def"
                     /locus_tag="PLB33_01090"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOG62145.1"
                     /translation="MALRNLRYEGDEILRKKSREVPLVDDRIRMLMEDMVETMYKYDG
                     VGLAAPQVGILKRVIVIDVEDGKVYKMANPNIINESGEQSAQEGCLSVPEKKGIVSRP
                     MKVIVEYTDENNETVKLEAEGLLARAICHEIDHLNGVLFIDRVTDDVIEE"
     gene            40454..41380
                     /gene="fmt"
                     /locus_tag="PLB33_01095"
     CDS             40454..41380
                     /gene="fmt"
                     /locus_tag="PLB33_01095"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOG62146.1"
                     /translation="MNVIFMGTPGFAVPTLEKLHESKHNLVLVVTQPDKPSGRGKKLK
                     KSEVKETAEKLGLNIFQPDKIKKQENIDLLKSYNPDVIVVVAYGQILNKEILTLPKYG
                     CINVHASLLPKLRGAAPLNWALINGETKTGITTMQMDVGLDTGDMLLKSEVEIDENIN
                     VGELHDILMHKGAELLIETLDKLEKNELTPQKQDDSLSSYAPILNNENRKINWNLPAK
                     SIHNLIRGLSPWPAAYFTLDEKTVKVYSSSYINNDSDYEPGYVIKANNEGIFVKAKDG
                     IVILKEIQMPGKKKMPVEAYLRGNKFPEKIIL"
     gene            41660..42025
                     /locus_tag="PLB33_01100"
     CDS             41660..42025
                     /locus_tag="PLB33_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOG62147.1"
                     /translation="MKFTFNHFNFNVLDLEKSMKFYEEALGLKEVKRKEAEDGSFVIV
                     YLGDGKTNFSLELTWMKGRTEPYDLGEQEFHLAMAADNYDEAFKKHEQMGCIEYINEK
                     MGIYFIVDPDGYWVEVIRG"
     gene            42134..42745
                     /locus_tag="PLB33_01105"
     CDS             42134..42745
                     /locus_tag="PLB33_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62148.1"
                     /translation="MIERKLLKILSLSLVLSLSLIGFVFAETKENLDEPVSIQITAVD
                     EEMYEEQADIDKLLFEQNYANDLELKGIYITHTSTVDDYVEIGITPYTPENADAVVRL
                     LGKDNVKVVEGVLAEILQYSTDNPETIDPAELADDSQIVDVTADAEDISQPADEDVVE
                     DAEIISAPAEKEVEKNGISPFNILGLSAFVIGIVVVLFKKKSA"
     gene            42952..43302
                     /locus_tag="PLB33_01110"
     CDS             42952..43302
                     /locus_tag="PLB33_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOG62149.1"
                     /translation="MAEPVKANITLDLLDKIDIRVGMIISVEDVAKSDKLVKLTVDFG
                     EFTRNILVGMKKERDNPKEIEGKQALFVVNLAPKKMAGEISEGMLFDIGYADGITPVL
                     AQPEKPVPNGSRAG"
BASE COUNT        15652 a         6417 c         8465 g        12904 t
ORIGIN      
        1 aatgcaaaca atgaagcaaa agctttggct ataatatata atgcgtggga ggaattatga
       61 gtactgcaga taaaattttt aaagagatgt gtaaagatat attgactaag ggagtatggg
      121 atactgatta cgatgtaaga ccaagatggg aggacggcac gcctgctcac actattaaga
      181 agtttggcat agtaaacaga tacaatttac aggaaagttt tcctattcta acactgagaa
      241 aaacaaattt cagagctgca gttgatgaac ttttgtggat atggcagaaa aaatcaaata
      301 acgtaaatga tttaaacagc cgtatttggg attcctgggc agacgaaaca ggctccattg
      361 gcaaggctta cggttaccaa ctcggaataa aacataaata caaggaaggc gaattcgacc
      421 aggtagacag ggttttatat gatttaaaaa ataatccggc aagcagaaga attttaacaa
      481 atatttataa tttccaagac ctgcatgaaa tgcacttata tccatgcgca tacagcatga
      541 ctttcaatgt ttccggaaac aagttaaatg ccatattaaa tcagcgctct caggatgtct
      601 tggcagccaa caactggaat gtggtgcaat attcaatatt ggtacatatg atggctcagg
      661 taagcggact ggttgcagga gaattagttc atgtaattgc cgatgctcat atttatgaca
      721 agcatgttcc cataatagaa gagcttattg aaatggaaga gcatgagccg ccgacattgg
      781 aaatagataa tatagatgat ttttataaat ttactgtcga tagttttagg ttgattaatt
      841 accaatacaa tccttttaaa gtaaaggtac cgatagcggt ataaaggaga aaccatgaaa
      901 ttaatagttg cggtagcaag agattgggga ataggaaata agggagagct cttgttcaat
      961 gtgcctgaag atatggaatt ttttaaaaaa actacattga acaaggtcgt cgttatgggc
     1021 agaaaaacac ttctttctct tcccggcggg acccccttaa aaaacagaac aaatatagtt
     1081 atgacaagtg atgtgaattt taaaaaggaa ggttgcatca tagttaattc aaaagaagct
     1141 ttgtttgaag agttaaaaaa atacgagagc gacgatgtgt ttttaatagg tggaggtata
     1201 ttgtacaagg agctttatcc ctattgcagt gaagcttata ttaccaaatt tgacgccata
     1261 ttggaagcgg atacatattt gcataatttc gatgaagaca aggattggct tctcacttat
     1321 gcatcagaaa tacatgaaca caacggagta aaatttattt tcaccatata caaaaactta
     1381 accttaaatg acaaggggac gggggtactg tcataatttg ataatactcc gtccccttga
     1441 cccggagggg acggttctct ccggacaatt aaactaaatg acatcaatga aaccatcctc
     1501 ggtttaaaat aagtgctgaa atatctttct atcaacccac cttcataaga actaaaccat
     1561 tcccaaaact atttgatttt ttctcttaat gccatagatt cataatttgt ttgatgttgc
     1621 tgtaacatat cctcaaaggt ataaccacca cttaaaatca agtcctctgt ccttgtgtat
     1681 ggcatgtgta atgggcttga aaggtgtacg taactaaaga tattataagt atcataaata
     1741 tcactgtcag tcatgacctt atagcgataa agcacatagc cgccctgttc ttcagagcca
     1801 aagggataac ttaaagtacc tgttatcagt acggggtagt aggtgccaaa cattgtgtgt
     1861 tgtgtcattc tgtcacatat aaggcgggga aaatccgcag tatactctgt gccttttttg
     1921 ctgtaaaaga gaccgctgct gattatctgt ttatcatccg ccgctgtttc tcccgatatc
     1981 agaattccat cctgccagat tggccgtcca cccttccatt tgaagtattg cattacatat
     2041 accacatttt tcggtaactc gatgtagacc gtagtagaat ccatattttt tttacccggt
     2101 tcataagtat gagttatttg aatgtagttt ccatatcttt cctcatgctc aattctcttt
     2161 gcatcaaaca ttagcaactt actgaatgat tccacattca cagcatcact tagcatcata
     2221 aggtcttcat tgttgagata tcgcagggct tcagccggaa atcccatgtc aaggagatgt
     2281 tgccttgttt cagtagtttc tggatgcaaa tattcttgtg attgtaattt caggtgattg
     2341 gaaaaaacac tgcatgttat cacaactgca aatgcaataa gaaaataagt ccatccgaat
     2401 attttgttgc tgattttgat aggtgcattt gtcagaacat atccggtatc gtccatttca
     2461 tctcctatat ggtataatga gcgcacaata aaaatatagg acactaccat aggtataaaa
     2521 atcagccaac tattggaaag tggtgataga gcaataagat atgctgccat tgtccataaa
     2581 gacgcccaaa gtaatgggtc agtttccatt aacctgcctg cttttttaga agtctctttc
     2641 aaagctgcac ggaaaacgag atacatagat atttggaatg gaagcattac agagcctata
     2701 atgagattgg gatagtccat aatgtttaag ggcgttgaaa tccaagccag gtctatcagg
     2761 tgtaatgata gctttattat ggaaagtaac catgcgattt taaaatattt attttccttt
     2821 ctcaggctgc gaaagccaag aaaaatatga ataacaccta taacaggcaa aatatattgt
     2881 aggtatataa tattcaattt aaatgttgtc aggataaaac cccaggtgat aaagccaatg
     2941 ggtttgctcc agggatttgt ctttcggatt tcctcttccg gaggggtcac gtagctgagc
     3001 tgctcaatta tctgtttatc aaagttataa tctttttcgc tcatatgaaa tcacctccta
     3061 atttcttctg cagctttttt cgcagtcggt atagtcttcc ttcgacagag cgttctgtca
     3121 ttccaagttc tgcagcaatt tgagatgtgc gctgcaggta ataatattta cggtagaaaa
     3181 tatgctgttc ttccggagaa agtgtgcgta tagcacattt taattcctcc gcccgttccc
     3241 ggcgaaggac atcatcttct acagagtgtt tatccgctgt ctcctctttt agctcgtcat
     3301 gttcagtata cttgcttttc atataattaa gcgctgtatt acgtgatatt acggtaagcc
     3361 atgtggagaa tttgcccttt tccggagaat agctctcgat ggaattccaa actttaatgc
     3421 agatatcgct tatacattct tctatgtcgc tctcattttg caaaatccct cttactatat
     3481 aatgcatgag ccctgaatac tgttcttgca tgagcgttaa gcctttgtcc tttgatgatt
     3541 tcacaagctc aataatcttt tcatccacat gcccacctcc tccatcttcc attatattat
     3601 acaagtaaat ttgtagaatc ccacgaaata ttataaagtt cctgaaaatc gcaagatata
     3661 aatatggtac tgtttcttat ttttttaaga aacagtacct cagattattt acaaacctaa
     3721 tcttcctatt ttgaatttat ttgcgtaaaa tcttatccat tgcttttcct tttgccaatt
     3781 catcaatcag tttatccaaa taacggattt cacgcatgat tggttcttca acgttctcta
     3841 ctcgaacacc gcaaactaca cctttaatca gggttcttgc aggatttggc aggggagctt
     3901 ccaaaaagaa agtttcaaag tctgtttgtt tattaagctg ctcttgaaat tcttcttggc
     3961 tgtatcctgt taaccaacgg ataatttcat cgacttctgc ttttgtgcgt ccttttttct
     4021 ccgccttggc aacataaaag ggatagacct ttgccacgct cattttatag atagagggat
     4081 tggtcatgta agcctcctgt attaatatga tcgggacggg gatgtcattt atgacactac
     4141 ccccgtcccc ttgtcatctt ttatttatcg atatgaacat tcattttatc ttttaatgca
     4201 gcttgtgcgg cagcaagtct tgcaacagga actctgaatg gtgagcatga tacatagtca
     4261 agccctactc tgtggaaaaa ttcaactgag gcagggtctc cgccatgctc cccgcatact
     4321 cccaggtgca aatgttcctt tgcttttctt cctaatttta cagccatgtc tactagtatg
     4381 cctacacctt cctggtcgat agtttcaaag gggtcttgct cgaggattct tgcttccttg
     4441 tagtcctcta ggaatttgcc tgcatcatcc cttgaataac caaatgtcat ctgtgtgagg
     4501 tcatttgttc caaaggagaa gaaatcggca tattctgcta cttcgtcagc tgttaagcat
     4561 gcacgcggta tttcaatcat tgtgcctaca agatagttca aatcggaatt gaattccttt
     4621 ttagtttctt cagctgtttc gattattaat tttcttagta tcaatagttc ttcctttttg
     4681 cctacaagcg gaaccattat ttccggtata atttctaaat tttcttccct tgaaacttca
     4741 attgcagctt caattattgc tcttgtctgc atttttgcta tttcaggata tgaaattgcc
     4801 agacggcagc ctctgtgacc aagcatcggg ttgaattcgt ggagactgtt gactttggct
     4861 tttacttcct gtaaagaaac attcatagct tcagccaatt ctattaaatc atcttcctct
     4921 gtaggtaaaa actcatgaag cggagggtca agaagcctga ttgtaacagg acgaacgccc
     4981 attgctttaa atattccttt aaagtcgttt ttctgcatag gcataatttc tgccaatgct
     5041 ttttctcttt gctcaactgt atcagaaata atcatttttc taacggagaa tatacggctt
     5101 tcctcgaaga acatgtgctc agtcctgcaa agtcctatac cttgtgctcc gaatttaacg
     5161 gctgcttctg aatcatgggg tgtatccgca tttgttcgaa ctttaagtct tctgaattca
     5221 tctgcccatt tcattaattt ttcaaaattt cctgatatgt tggcaggtac tgtttttatt
     5281 attcccgcat atacgcttcc tgttgagccg tcgagtgaaa tataatcgcc ttcatggtaa
     5341 actgctttgt ttacagtcat tgttttattc ttttcatcta ctgataccga ttcacagccg
     5401 gcaacacagc atttacccat acctcttgca acaactgcag catgagaggt cattcctccc
     5461 cttgaagtca gtatgccttt agctttgctc attccttcaa tatcttcggg agatgtttct
     5521 tttcttacaa gaattgcatc ttcacctttt aatgaagctt caaccgcatc ttcggctgta
     5581 aagtatattt tcccggaggc tgctcccggt gaagccggca gcccttttgc aataggtgat
     5641 gattttttaa gctctacagg gtcaaactta ggatgcaaaa gctggtctaa tgatttcggt
     5701 tcaactctta aaactgcttc ttcttcagat atcaatcctt catcaaccat ctcaaccgca
     5761 gctcttaatg ctgcatcagc tgttcttttg cctgaacgtg tttgaagtat gtagagtttg
     5821 ccttcctcta ttgtaaactc tatatcctgc atgtctttgt aatgctgctc gagtttttcg
     5881 caaatattta tgaattcatc atatatttca ggcataatat cctttagctt atcaattgtt
     5941 agaggtgtac gtattccggc aacaacatct tcgccttggg catttatcag gaattcaccg
     6001 aatactttgt cctctccggt ggacgggttt cttgtgaatg caactcccgt tcctgatgtg
     6061 tctcccatgt tgccgtatac cattgactga acatttactg ctgttcctat atcgtgaggt
     6121 atattgtata aattcctgta tgtaatagct ctttggttat tccatgattt aaatactgct
     6181 tctactgcca ttaaaagttg tttttccgga ttatcgggaa aatcttcacc tacttcattc
     6241 ttgtaaattt gtttgaattc ctgcacaagt tcttttaaat catctgcagt taattcagta
     6301 tcaaatttgt aaccgttttt ttctttttta gcttccagag cattttcaaa caaagattta
     6361 ggaactccta atactacatc gctgaacatt tgaatgaatc ttctgtaact gtcgtatgca
     6421 aagcgtgtat ttcctgtttt tcttgcaata ccttcaacag tatcttcatt taaaccaagg
     6481 tttaaaattg tgtccatcat tcccggcatt gaaaatttag ctcccgagcg gacagatacc
     6541 aaaagagggt tattagtatc accaaatact ttttctgtct gtttttccag aatagataaa
     6601 ttagtcctga tttggctgat aatttcctca gataattttt ctccttgatt gtaaaattcc
     6661 gtgcaagcct ccgttgtaac tataaatcct tgaggtacgg gcattcctat attagtcatt
     6721 tctgctaaat ttgagccctt tcctcccaat aagctttttt gagaagcact tccttctttg
     6781 aataagtaaa cccatttttt actcatatga acctccttct atattcctat tattattgca
     6841 aactcattaa ttgagtatta ttcctaaaat aaattatata gaccaataat attataaatt
     6901 taataaaaag tacagtatta tttcaaaaaa tataaaataa tataccggac tgacatataa
     6961 cggcaaggtt aaataaggct tgttttaatt tttatatgtt gtataatatt accataagct
     7021 acctaaaaaa atttttggag gtttttatgt tttcaacagt aaatgaagtg caagagtatt
     7081 gtgtcagtaa caaaattaaa atgattgact ttaaaatgat tgacataaac ggacgctggc
     7141 gtcatttaac tattcccgcc gaaagactta acgaagatac aatgaagtat ggtatcggtt
     7201 ttgacgggtc aaattacggt tatgctaccg ttgagaaaag cgatatgata tttattcctg
     7261 atctaaccag cgcacatatc gatccttttg ttgatatacc tacactttct atgattggaa
     7321 atgtatttgt tttacatcct gacgggaata aaccctttaa tcaatacccc aggaatgttg
     7381 cattaagagc cgaagaatat ttacgagaaa gtaaaattgc tgattccatg attattggtc
     7441 ccgaatttga atttcatgtt ctggatcata ttagttatga atgtaaacca aacaaggtag
     7501 cttttgagat ggatacatca caagctgagt ggaacagcgg taataatgaa agcagaaatt
     7561 taggttatac cgtaccttta aaaagcggtt atcatataac tgcgcctcat gatataactt
     7621 acggactaag gagcaggatg tgtcttcttt tggaggattg gggcgtagaa gttaaatatc
     7681 accaccatga agtaggcggc cccggacagg ttgaaattga ggttgagctt ggagaattaa
     7741 ctgaaatggc cgacaaaacg atgataataa aatatgttgt taagaattcc gccatagcag
     7801 aaaacaaaac tgcaactttt atgcctaaac cgttttttgc ggaagccggg aacggcatgc
     7861 acgtacatat gctcttgatg aaggatggaa aacctgtttt ctatgatgag aacggatatt
     7921 ccggactcag ccgggaggct cattggttta taggaggttt attgaaacat gtcaggtccc
     7981 tttgcggttt gacaaatcct tccacaaatt catacagacg ccttgtgccg ggatatgagg
     8041 ctccggttac gataggttat gcaacttcaa acagaagtgc agttgtacga atacctgcgt
     8101 attgcaaatc gcctgaagca aaacgttttg aaataagaaa tccggatgga acatgcaatc
     8161 cttattatgc ttatgcagct atacttatgg caggtataga cggtataatc aataaaatag
     8221 accctcatga aaacggctgg ggtccatatg attttaatct atacaatctt tcacctgagg
     8281 agcagaaaaa actttcagcc cttcctcaat cattggacga agctcttaaa gctctggaag
     8341 aagaccatga ttatttgctt cgcgggggag tattccctga agaattactg aatacatgga
     8401 ttgcaaataa aagaatggaa atgaacgaaa tcaataaaat acctcaccct gctgaatttg
     8461 cttattatta tgatttgtag ttgttctatg aattagcgcc tttccgaatg cacattatta
     8521 cctccaaagt agataatgta attaattatt ttacaaaatc tatcaggagg taaatttttt
     8581 gtatacagaa atcagtattg tcttagtata tgtgggattg gattaattga atatattatt
     8641 atagctttga cataataatt caaacataat ataattataa aaaacaaaga gcttgttgat
     8701 agcgttcatt gcatggtaat gcaaatgaat aatgcatttt aaatacgtat agtgaggtta
     8761 aaatgaaatt actggatagt ataaattcgc ctgaagatat caagcatttg aatatggacg
     8821 aattaaattc tctttgcagg gaattaagag aatatatttt agaaaacgtt tcaaaaacag
     8881 gaggacatct tgcatctaac ttgggagtgg tagagctcac tgtagccctg catcgagttt
     8941 tcaatactcc tcatgataaa ataatatggg atgttggtca tcaaacgtat gcccataaaa
     9001 ttcttactgg aagaaaaaat aaaatgtcat ccttgcggca gcttaatggt ataagcgggt
     9061 ttcccaaaag aaatgaaagt atttttgatg tgtttgatac gggacacagc agcacctctg
     9121 tttctgcagc aatggggatg gcaagagcaa gagacttaaa taatgaggat tatgaaattg
     9181 ttgcagtaat aggagacgga gcccttaccg gaggaatggc ctttgaagca ataaacgacg
     9241 cgggaaggag caacacaaaa ttattgataa ttttaaacga caatgaaatg tccatatcca
     9301 aaaacgtagg aggcttgtcc cgctatttaa gcagaataag aactaccaaa ggatatttgt
     9361 ccactaaaag ggatgtcgaa aaaatattaa ataaaattcc ggtcggtaaa cagattaaat
     9421 catttttaaa aagagcaaaa gacggaatta agcaaatagt tgttccgggg atgctttttg
     9481 aagaattggg gcttacctat atgggaccta tcgacggtca caatatacat aaaattattg
     9541 aatccctgga aagggcaaaa aatattgaag gccccttatt aatgcatgta attacgaaaa
     9601 aaggcaaggg ctataagttt gctgaagaaa gaccgaatga attccatggt gtttctgctt
     9661 ttaatataga aacaggagaa gctctaaaaa aatcctctca atcttattcc gattattttg
     9721 gaatgaaaat gtgtgaaatt gcggaaaaaa atgaaaatgt ggtcgcaata acagcagcta
     9781 tgactgatgg tacggggctt agggattttg caaaaaaatt ccccaataga atttatgatg
     9841 caggcattgc agagcaacat gcggtgacaa tggctgcagg gcttgccgta agcggggttg
     9901 tcccggttgt tgcactttat tcgtcatttc tacaaagagc ttatgaccaa atcatccatg
     9961 atgttgcctt acaaaacctt catgttgttt ttgctgttga cagggcaggt ttggtgggaa
    10021 atgacggcga aacgcatcaa ggtgtgtttg atgaaggatt tttgagttta attcccaata
    10081 tgacagtatt atcacccgct gattacaggg aattcggaaa tatgatggaa tttgccgttt
    10141 catataacgg acctgtagca ataagatatc cgaggggcag catgagtgaa tatatcaaaa
    10201 acagcgagca aagtatagtt ctcggcaagg gatacatagt cgaggaaggg aaggacataa
    10261 caatagctgc agccggtaaa atggtaaaga cggcaataga aacaagggaa attttaaaaa
    10321 caaaaaatat agatgcagag gttcttaatt taagatttat aaaaccattt gacaaagagc
    10381 ttataataaa ctccgtaaat aaaacgaaat cattggtaat aatcgacgaa gcaactgttt
    10441 ttgcttccta tgcagttaat atttacaata tacttcctaa aaatcttgat gtaatggtta
    10501 aaactttgcc cgatgaattt attaaacaag gctcgattga agagctctta aaagaaaatc
    10561 aattagatgc tgattcgata gctgctgaaa taattaagtg gaaggcagat aattatgata
    10621 ttgatataag caataattat acttgcttaa aacaaggacg ttagagcgga agtagaatta
    10681 ttaaattagt gaaaatgtat tatttactat tgaaatttta agggttttca tttatattat
    10741 agttacggat atattttact cacgataaaa aataaagggg atttgaatat gaataaagta
    10801 gaaattgttg ttggagataa aaaatatagt gtaagaactg atgaaagtcc cgaatatgta
    10861 agaaaaatag aatctgtttt gaacgatcaa attaatataa tagctaatca gaataagcgt
    10921 tttaatgaaa tagacaaatt aattctgtca tcttttgtgg ttattgacag gtatttaaaa
    10981 ctttccgatg aaattaacga atacaaaaaa gatatatgtg aagaaattct atctttaaag
    11041 gaagcaaaag aattatccga aaaggaaaag gaagaagctg taaataaggc agcggatgcc
    11101 attattgaaa aagaaagatt caaagaaaag ctaatggcaa gagacaatga ccgtgaatat
    11161 ttaaattccc aaataacaaa gcttcaggaa aaagtcagtg agcaggaaca gcagctcttg
    11221 aagagcgaaa tgctcataaa tgaattaaaa ataaagaacg aagagttgat tgaagtaaat
    11281 gaagagctaa tcaaagaaag agaaaacttc acaaaagaaa taagctttat gaacaataca
    11341 aaagcaagct taaacgggag aatttcaaag ctgcagttaa aacttaacga gaaagaacag
    11401 gaaataatca aacttgaaaa gactatcagg gatttaaaaa gcgcagtaga tgataaatca
    11461 caaaagatat ataatttcag cgatgagcag caaaaaatga acttattggt tgattcgaaa
    11521 gaaaaagata tcaattcatt aataaataaa atcaatttgc tgcaaaataa gcttaatgaa
    11581 aaagatgaat caattgcttc caaggataaa ttaataaatg atttaaagag cactgaagaa
    11641 gtattaaaac aaaaatatga atcgattaat gatgaaaaag aaaaatattt agaagagctt
    11701 ttaatgataa acagcgataa agaaagcctt atcaacagca taaatcagct gcaggaaaaa
    11761 ttaaaccgca aggaagcgga aaacttccag aaccagttgg aaattaataa gctgaaaaag
    11821 gataatatgg agcttatgca gcttttagac gaagaaacag caaaatagaa cgcgtcggcg
    11881 ttctttttta aacaggagaa gaaatgagcg tagaattatt agcgccggca ggcagtattg
    11941 aatcatttta tgcagctgta aacagcggag cagattcagt atatttaggc ggaaagtttt
    12001 ttaatgcaag atataattcc cggaattttg ataatgatga aatgaagcac attgtaaaat
    12061 atgctcacaa caagggtgta aaagtatatg ttacaatgaa tactgtttta aaggataatg
    12121 aaattacgga tgcattgaac tatgcaatat ttttgtatga aaatgatgta gatgccttaa
    12181 tagttcaaga tttaggtttg ttctgcctgg taaataaata tttacccgat tttcccgtaa
    12241 acattagcac acaggcaagt gtatacgatg aatacggtgt taaatttttt gaagaattta
    12301 atgtcaaaaa agttattatg gcaagagaac tgtccttaac agagctaaaa gagacagtta
    12361 aaaatacaga tgccgatttg gaagtattca ttcacggtgc cctttgcaca tgctattcgg
    12421 gacaatgcta catgtcaagc tttttgggtg gaagaagcgg aaaccgaggg aaatgtgcac
    12481 agccgtgcag attaaactac agcttttatg acaaagaaac aaaggaagaa attgaaagtt
    12541 ttaaagaggt gcctctgctt agtttaaaag actttatagc aggggaaaca atttatgagc
    12601 ttattgaagc aggaattaaa accttcaaaa tagaagggcg catgaaaggt cctgcatata
    12661 cttcatcagt tgtggaatat tacagacaaa taatagataa ttattacagc ggcaaaaata
    12721 ttgacagtac tcatctaaaa gaaaaggcta tggaaacatt cagcagaggt tatacaaacg
    12781 gatatttaaa gccttcaaaa gacgatgaaa tgtttgcgaa attaagttcc ggcataaagg
    12841 gagaaaatat cgatgaaata gaagaagacg taaaggataa ggcacaggaa tattcaaggt
    12901 tcaggagaaa taaaataagc tttgacatag agcttgtgat aggcgaatat gccctgttaa
    12961 aagtttttga cgggaaaaac acggttgaag taaaaagtaa agaggtctgc gagagcagca
    13021 ttaaaaacaa tgtaacagat gaaataattt atgagcagct ctcaaagtta ggtaatacta
    13081 tatatgaact gcatgattta aagattgtaa aagatgaagg tgtttttatc agaaaaagtt
    13141 ctttaaatca attaaggcgt gatgcgacgg aaattcttta cgaaaaatgc agcaaatttt
    13201 atgacagaag aaaagttaat actcttacat acaatgatat attttcaaat atcaataaag
    13261 aaagtcactc tgcaaaaata agccttaaaa taaacagcat tgatgaatta aagtatgctg
    13321 acgatgttaa ggttaagcgc ttatatgtgc ctttttattt aaatattgaa gaagtaaaaa
    13381 atactaaagt tgtagaaaaa tatttatggg ttccaaatat agtatctaaa gaaaattata
    13441 aaattttaaa agaaaatttg aacttgtatg atgaaatatt tgacggtgta tgtgtaaaca
    13501 atgttggaag tttttatttc tttaagaaaa atagcaggct gaagcttcac tgcggaccgt
    13561 tttttaatat tatcaatact ttttcagcag aacttttaaa ggaaaaggga gctcacagct
    13621 ttacattttc tgtagaaaca aatatcaggg acatggaaag tattgtgaaa cacacttcac
    13681 tcagctcgga aattgtcgcc catgaatatg tacagcttat ggttatgaaa aattgtccca
    13741 tgtcggtgat aaagaattgt aaaaacctgc aggactgtga aaaatgcagc ttcaaaagca
    13801 aatatcaatt aaaagacaga aaaggaattc attttaatat cgaaagacaa aataaactta
    13861 ctcatatata taacagcgtg cccctgtcga ttataggaat tgcaaataat ttcgttgaaa
    13921 aaaatattga ttatttttta gtcgatacaa aatttctaga ggatgcggaa gaaataatag
    13981 atacattgta ctgcgaaata aagggagtta aaactcatta tattttggag gaaaacaaat
    14041 ttacgagagg gcactatttg aagaatgtgt tgtaacacac ttgtcatcct gagcaaagcg
    14101 aaggatctca tgagattctt cgagctaaag ctctcagaat tatcacgtgt ttatcacacc
    14161 acgataaacg aatattctct gtgattgtca tcctgagcaa agcgaaggat ctcatgagat
    14221 tcttcgagat aaagctctca gaatgacaaa atgctaatca tagcaatgac aaacaggaaa
    14281 ggaaaaacga tgaaagaaaa atctaaatca gttttggaat ttgataaaat aattgaaaag
    14341 gttgccggat ttgctgaaac caagatggga aaagaagagg tgtacaaatt agatacatct
    14401 cacaacctgg aaggtgtaaa attcaagcag gagcaaacag cacaggcact ttccataatc
    14461 attgaaaaag gttcacctcc cttgggaggt atttcagata taaaggaata cgtgaaaaga
    14521 ggagccgtag ggggtattat atctctaaga ggactattga actgtgccga taccctaaga
    14581 gctgcaaggc ttctaaaaaa ttatgtcctt ctgaataatg acgacagaaa ttatgatttg
    14641 ctggaagact tgtgtaacag tatatatgta aacaaggata ttgaagaaag aatatattcg
    14701 gttattatca gcgaggaaga aattgccgat gatgctagcc cggagcttaa gcgcataaga
    14761 agagaaattc aaataaaaaa caattcaata aagaataaaa ttaattcaat cgtaagctca
    14821 tcttccatgc agaaatatct gcaggaagca atagtaacca tgcgcaatga cagatatgta
    14881 atccccgtga agaaagaata cagaagtatg gtcaaaggaa taattcacga ccagtctgca
    14941 acaggctcca cgctttttat agagcctatg gcccttgtgg aaatgaccaa tgatattgcc
    15001 aatttaaaaa tcgaagaaaa aaaggaaatc gagcgtatac tgttagagct tagcatgatg
    15061 ataggtgaca taagtgaaga aatgctaaac aatcaagaaa tactaaaaga gcttgacttt
    15121 atttttgcaa agggcaagta cgccataagc ataaacggaa ttgagcccaa gataaatgac
    15181 agaggatata taaggataaa aaagggaaga catcctctca tttcacctga attggtggta
    15241 cctatcgatg cagtgcttgg tgacgaatat acaaccctaa taataacggg acctaatacc
    15301 ggcggaaaaa ccgtttcatt aaaaacctta gggcttttca ctcttatggg aatggcaggg
    15361 cttcatctcc ctgccgaata cggcaccgaa atatctgttt tcagcgcagt ctatgccgat
    15421 ataggcgatg agcagagcat cgagcaaagc ttgagcacat tttcctctca tatgacaaat
    15481 atagtgaaaa tattaaaaga ggttgatgaa aagtcattcg ttcttttcga cgagctcggt
    15541 gcgggaacgg accctactga aggtgctgcg ttggcaattg caatacttga cactctccat
    15601 gaaagaaaga ttctgactgc tgcaaccaca cattactcag agcttaaatt atacgctctt
    15661 acaaccgaag gagtaataaa cggaagtgtt gaatttgatg ttgaaacatt aagtccgacc
    15721 tacaagcttt taattggtat acccggtaaa tccaatgcct ttgaaatatc aaggaaacta
    15781 gggcttcagc aagaaattat cgataaggcc aaaaagaaca tagaaaccga gaaaattgaa
    15841 tttgaagatg ccttaaaaca aattgaagaa aaccgaattt atatcgaaga gaagaaacaa
    15901 gaaattgaca gactcgaatc agaaagcaaa aaaaagcaca gcgacttttt gatgaaggaa
    15961 agaaaatcac ttgaaaaaag tgaaaaaatt atagatgaag ctaattatga agcaagaaaa
    16021 atagttgaga atgccaaaag ggaagctgct gaaattatca aggaattaag aaaattaaac
    16081 cttgaaatgg ataaggataa aaaccgccgt gtaaacgaat taagacaggc actaaatgag
    16141 aaaacaaagg agctttcaga aaatatttat tcggatatta ttgaggaaga agatacaatt
    16201 gacacagaca cacctttaaa aatcggtgac gccgtaactg taagaagtct aaatcaaaag
    16261 ggattcatta tttctgatgt ggatgattca cagactgtaa tggtccaaat aggactaatt
    16321 aaaacaaagg tcaaaaaatc cgacctaata aaaataaaat ctgatgaaga agtaaaacaa
    16381 aaaaccaacg catcccgtaa ggtcagacta agaacttcgt caatcaatcc tacaattgat
    16441 atcagaggat acaatcttga tgatgctttg ttggagctgg ataaatattt ggatgatgcc
    16501 tttatgtcaa atttaaacga agtccaggta attcacggta aaggtaccgg ggtcctgcgt
    16561 gaaggagtaa ggcaattctt aaaaaatcac aagcatgtta aggaatcaag acttggcacc
    16621 ttcaacgaag gcggcgatgg tgttacaata gtaaaattca agtaaatgac aaggggacgg
    16681 gggtactgtc ataattcaga ggcggtagct gtcttaaggg attaaattat gaatgtacgg
    16741 gataaataca gtaataaagc tgtaataaag tatacgggag cttttatagc ttgggttatc
    16801 ggttcgggat ttgcaacggg acaagaagct ctgcagttct tttcaagtta cggatactta
    16861 agctatgggg ttcttctggt taatttgata ggttttttat ttttggggca agtattatta
    16921 gttacaggat ttgaacataa aaccgaaagt tcatttaatc attttaaata cttttgcggc
    16981 gagaaaatag gaacaattta ttcttggctg attccaatta ccttgatttt aattatgcct
    17041 gtcttaatct ccggtgccgg tgccaccctt tccgagtatt atggagtaaa tcgctatgtc
    17101 ggttcttttc ttatgtccgt cttggtttta agtgtctatt taattggatt tgaacattta
    17161 ataaaaatag tttcagccat aggacctgct attattattt tttccttgct tgtgggagcg
    17221 ataacactag caaatgatta cggcaatttt tctgatgtac caaaatatca atttaaactc
    17281 aacgagttgc aaacagctcc acattggacg ataagtgcta ttttgtactt atcgctgaat
    17341 ttcttaagcg gcagcgtata ttttactgag cttggcaaat cagcatataa tcgaaatgac
    17401 gtaaaatacg gagcattttt tggggctctt gcaataattt taaatattgc aattatgaat
    17461 actgcaatct tattgaatgc tgaaaacata tcaacattag caataccggt cttatattta
    17521 gcaaaaaaaa tatcttatat gttaggagca attttttcaa ttatattaat attaggtatg
    17581 ttttcttctt gttctgcaat gatgtggtca atatgcaacc gatttacaag aggcgggaaa
    17641 agaggcaaca ggatttttgc agtctttatc tcgatattaa cctttgtgtt agggttattc
    17701 tcgttcagtg aacttatagg taccttttat cccctcgtag ggtatgttgg attgtttttt
    17761 attggttctg taatatataa aggaattaag tataatatta ttaagaattg aggtgcataa
    17821 tatggaaaga ataataaaag atgtaaattg tcccaaatgc ggacaagtga tggaagaagg
    17881 atatattcat tcacgaaagc aaatattatg gtccgaagat gataagccaa gactgtttga
    17941 tttctatgat gaaaatttag tcacaatgcc tataagaaga gcaaataaag ttcccgcatg
    18001 gagatgcaag gaatgtaaaa ttgcaatatt ccggtatgaa taattatgat ggtataatat
    18061 ttagtaaatg ctaccattta tgattaatcc atgatacaat atttaataaa aaggtaaatt
    18121 tattattaat attaaataaa aataaaagaa agggagtgta aaagatgaga caatatgtag
    18181 ttgatgcatt tgcagaaaag gtttttgaag gaaatcctgc agcggtatgc attttggatg
    18241 agtggctgcc ggatgaagtt atgcttaata ttgccagaga aaacaactta gccgatactg
    18301 cttttgtggt taaggatggc acaaatttca aattaagatg ggttactccc ggcggtgaaa
    18361 tagatttatg cgggcatgca acccttgcaa ccggatacgt tattagcaga tttattgctc
    18421 ctgaattatc ggaagttgtt tttcaaacca aaagcagcgg aatattgaaa gtccttaaaa
    18481 aaggtgagct ttatgaaatg gattttcccg cttataaatt aaaaaaggta gaagttacaa
    18541 atgcaatggc ggatgctata ggagttaaac ctatagaagc atatatgggg agagatttac
    18601 tctgcgtact ggagtccgag gatgaagttc gaaatttaaa tccgaactta gaaaaggtaa
    18661 aattactgga cggattgctt tttcatgtta cagccaaagg aaatgaattt gattgtgtat
    18721 caaggtcatt tgtacctaag cttagcggta ccgaagaccc ggtatgcggt tcggggcatt
    18781 gccatataat cccttattgg gccgaaagat taggaaaaga caatttaatt gcttatcagg
    18841 catctcaaag aggcggtacc ttgtattgta aatatcaagg ggacagggtg ataatgggag
    18901 gcaaggtagc attgttttcc accgcggata taaacataga atagtttttt atttacataa
    18961 agaaaatatt attaagatga aacacataat atcgatatta aatatggagt acttatggaa
    19021 agagaaatag taagattgga tatggataga gattttgagg catttgaaga tttattgaaa
    19081 atctctttcg gtgaagcaac caactcaaag aaagaaatgt atgaatggct ttttgaaaat
    19141 aacccttaca ataaatccgg aaatatgatg tatttattaa aggaagggga taaggttata
    19201 ggttgtgacg gattgttgcc aaacgaactt tatgttaacg ggaaaacttt gttaaccgct
    19261 cattcggtta aatccatgac ccatcccgat tacaaaagac agggtatttt cagaaaaatg
    19321 acggaaaact cctgtgagag gggaaagcag gacggtgtgg atgtgataat aggtttggca
    19381 aacgaccaat cttatccggc ataccaaaaa ttcggctggc ctacactttt tgaaaaagaa
    19441 gtatatgtaa agcctatact tattaacaat attcttaaac gaagaattaa aattggtttt
    19501 ttgtcagaag cagccaattc tgtctatact gtatttatga agaataaact aaaaaatcag
    19561 atggacaaag aaattaaatt tgaaatactt gacagggttc cggaagaaat tcaaaaatgt
    19621 tgggacagat acaaatcaaa atataatgtt cttttggtca gagattataa atacctgaac
    19681 tacagatata acgaaagacc ggacattaat tatgtaacca ttcttgcaag gcataagggg
    19741 gagattatcg gctttgcaat acttcataat gcagttgcaa acggctctaa tatgacatct
    19801 gctgtagaat tttttactga ccccgataat gaaagataca taaaagcatt ggctaatggc
    19861 atagcgaagt attgctatga caagggactt gaatatgccg ttattggaac ggggcattac
    19921 ggtgaataca ggaaggttct tttgaataat ggatttatgg taacccgcaa acctccaaag
    19981 aataatatga tgattgctca tatattatct gacaaagtaa ccttagagga aataacaggt
    20041 catgaaaagt ggcatataac tcaaggagac ggcgaaacag agctagactt gtaactgtat
    20101 tgtatccgtt tttataagag gttttcaaat agcagtaaaa tactcctaat acgcctctat
    20161 aattatttta aaatatatta gtcaggaaaa accaagcttt ggtttttcct gagttctttt
    20221 atatttataa gcaccactcc ggaatttatg taggtgcttt tctcaggcat atcaagccta
    20281 agctgcaaat aatgtttgtt tacagttaaa attcatgtta aaatatagtt tgagattata
    20341 tatataatac aattattcat atgtaatggg gagaaggtaa taatgattaa attaatagtg
    20401 gacagcacat gtgatttgaa ttgtgaaatt ttggacaacc atgatgtaga ggttattccg
    20461 ctaagtatta ctattgatga caaaacctat ttagatggag tcgatataac tacggacaga
    20521 gtttatgagt atatgaaaga cggtaaagtc cccaagactt cacaagtgtc atttgagtct
    20581 gtatcccaag ccgttgaaaa gtgtatttca aaagaatcag attttattta tttggctttt
    20641 tcatccaaat tatcaggcac atacagtttt gccaagaaaa taatagatat gtataaggaa
    20701 aaatatcctg agagaaaaat ggcaataatt gattctaaag gcggagccgg agggaccggt
    20761 ttaatagcta tacaagtact taaaatgatt gaaagaaact taccctttga atatatctta
    20821 aaccaggcac aattcatggt tcaacatata gtttacagat ttacaatatc tgatttagaa
    20881 tggctgtcaa aaggaggcag aataagcaag tctgcaggat atgtaggaaa tgtattaaat
    20941 attaaaccat atctcgtttt agatgatgga cttattaaag tatccaaaat gattcgcggc
    21001 agcagaaaaa ctattcaaac tttgattcaa gatattgaag acggtacaga taaatttaaa
    21061 aatcagatta tcggcataag tcatgcggat gatttagata ctgctttaat ggtggaacaa
    21121 aaaattaagg attcaattaa aggatgcaag acaacaattt ttaaaatcgg tgccgtttta
    21181 ggagttcatt tgggaatagg cggtgtagga gtttttttct ttgatgaaaa acctgaattc
    21241 tatgaattat aatattatta atagtatata aaaatagtta ttgcattagt tgacacatta
    21301 aaaatgctgt gatattatta caaaaaatag aagttacaac ctgccgaaca tatcaaatgg
    21361 taggttttag ttttaataat tcgtaattac cttggaggag agagatggat tttaaggaaa
    21421 aagtggctga aatttttaca tccatagaaa ccgagctgtc aatggatgat gctcttcaat
    21481 taatagagat accacccagt agcgatatgg gagattatgc ggtaccgtgt tttaaatttg
    21541 caaagaagta caggaaatct cctgcagcaa tagcaaatga aattgtagaa aaaataggag
    21601 cagttgaaga attcgaaaaa attgaaagtg caggaccata tgtaaatttc tttgtaaata
    21661 aatctcagtt tgcaaaaaaa atacttaaac aagcttttga agaaaaagaa aattacggca
    21721 gaacgaatgt gggtgaaggc aagactgtta tagttgagtt ttcatctccg aatattgcaa
    21781 aaccgtttca catagggcat atacgctcaa cattaatagg aaatgccata aataatattt
    21841 ataaatacct tggttataat actgttgcaa taaatcattt gggggattat ggaacacagt
    21901 tcggaatgct tatttcggca tataaaaaat gggcaactcc tgaaataatt gaagcaatcg
    21961 aaaaggaccc catacctgaa cttttaaagc tgtatgtaag atataatcag gaaattgaaa
    22021 cacatccgga ataccaggaa gaagcaaggt actggtttaa tcaattagaa aaaggcaatc
    22081 aagaagcata tcaattatgg gcatggttta gggaagttag cttaaaagaa tttaaccgcg
    22141 tatatgatat gttcggtatt aagtttgatt catatgccgg agaaagcttt tattcggata
    22201 aaatgcctgc aataatcgat gaattggaag ataaaggact gcttaaagat tcggagggag
    22261 caaaaatcgt agagctttcc gagtttggtc tgacggatgc attaatccaa aagagcgacg
    22321 gcgctactct atatgtaacc agggatgtgg cagctgcaaa atacagaaaa gaaacatata
    22381 atttttacaa aaatatatac gtggttggtg cacagcaaaa attacatttt gaccagtgga
    22441 gaaaaataat agatttaatg ggatatgact gggcatatga ctgtgtacac gtaatgtttg
    22501 gaaccgtaag cctggaaggc ggtgctatag ctacaagaaa aggaagagta gtatttttgg
    22561 aagatgtttt gaataaagca attgaaaaaa ctaccgaaat tattaatgaa agaaatccgg
    22621 gcttggaaga cagggagaaa gttgcaaagc aggttggtat aggtgctgta atattccaag
    22681 agcttttcaa taacagaata aaagactatg tattttcttg ggataaaaca ttaagctttg
    22741 aaggtgaaac aggaccttat gttcagtaca catttgcaag aacctgcagt cttcttagaa
    22801 aagcagatat tgatataaat gatgatgtgg attattctgt tttgacagac agtgaatcat
    22861 tcaatgtaat taaaaaactg gaaggtttta aagaggcggt tttaaacgct catgataaat
    22921 acgagccctt tatgattacc cgatatattg tcggccttgc acaagaattt aaccgattct
    22981 atcatgaatg tcagattatt actgacgatg aaagtgttaa aaaggcaaga ctgcttctta
    23041 ctctcacagt caatacagtt cttaaacaag gtatgaagct tctgggaatg gaagcaccgg
    23101 agaagatgta actacgtaaa cttcggtgcc aggcaccaat tttggtgcct ggcaccaatt
    23161 ttttttacta aaacgtcaaa ttaactccct attcaacaat acatttcaag tttcaacatt
    23221 gagcataaat ttaatttaca ttataattaa tattggacaa ggaggtacga aatgaaaagg
    23281 gtaaattatt tgactttttt aatatttcta tgtatatttt cttactttat gatgataaat
    23341 acaagcatga ctgcaatagc agaggataat caaaattatt acaaattttc acaggataaa
    23401 ataataaaat caattattca tgattacagg gattcattta ttcgcgaaaa agaattgatc
    23461 cggtatacag aagaactgac gggattagat tacgagcact gtagatttct tattaaagaa
    23521 agcaaagaaa aatacataga cccctttatt gtattgggtt taataaaaag agaaagtgat
    23581 tttaatccca aggcaaaggg tgtcggagga gaattgggtt tgggtcagct gatggagaat
    23641 actgcccaaa ttattgctat gaatttagga tattcatata ataatgaaat gcttttagac
    23701 ccggtattta atttaaaact aacaattgca cagctttcat atcttaataa agtttacgat
    23761 aatgacatgc ataaaactct cactgcttac aacagaggtc agcaaggact caaaaactat
    23821 ttgacaacat gtgatgggag aggtgttagt gactactcta ctaaggtttt acaatttgca
    23881 tccatttata aagaagaatt tgaaaattta aagtattgat taatagcatt tgataatttc
    23941 aataattgat tgtataatgg aatcgcagtt aatactgcga attctttttt atgacctgac
    24001 tccattgctg atgtaatagc aaaacataaa gggataaggg gtcaaacgca aggaatgcca
    24061 taaatttgcg gcattccaat gcgatttagg agaatatatg caatataaaa taaacaagat
    24121 gctaaatttc tgtttcgacg acatacaaaa aattgacttt cagctctttt taattacggt
    24181 aaaatgtctc ataccctatg atgataaaat cggatgcctt tttgataaag atagatataa
    24241 aaaggaaatt gaattatttt cctcttatat gaatggtaat gatgaaacaa ttgattactg
    24301 gctgcgaaat aaagaggcgt caaatttaga tgacaatctt ttagagtata aaatcattcc
    24361 acttgccttg tcaaacactg tgtgggaaaa tttaacggat gaacttataa aagtggtttt
    24421 attctacaca ataaataaaa atgccatatt gaatgctttg cttatttcat ctgcagtttt
    24481 tgattttatg acaggctctg atattgacag tattgaggca gcagcaaggg aaaggcttat
    24541 aaatttttcg gtaaaagaat ttttaataaa aaatcaaata aatgcagata aaaattatat
    24601 aatcgatttt gaaaaagaaa gaataaaaat gattgctaag cctatactgt tcgataataa
    24661 ttttatcaac ggattcaagg tcttaaatta catatttgaa aagagcccgg aacatttaac
    24721 cgaaaataac tcggatataa tagacagctt ttctgtatat ttgtacaaac tcaggaaggg
    24781 tttaataagt cctgaaaaat taataatacc ggataaaatt ccggaaataa aggaatgctt
    24841 aaagtattcg acattcagcc atcccctgtt agggagatgc aaggttataa aaagaaccga
    24901 aaaagaagca attgttagga ataaattggg gctgatgagg attagaatat gaaaatattg
    24961 ttggtaacct tagattcaaa atttattcat gctaatttag cagtgcgata tttaaaaaag
    25021 tattgcaggg gttttgatat tgtaataaaa gaatttacaa taaatcaaaa aatcgaatac
    25081 atactggatg aaattttggc tgaagatgca gatttaattt gtttttcctg ctatatatgg
    25141 aatattgatt atataaagga tatttcatac ataataaagg aatcaaataa taaagccaag
    25201 atattgtacg ggggtcccga ggcttctttt gaaataaaag aattgatgga aaacaacccc
    25261 ttcatcgatt atgtaatatt cggggaaggg gaagaaacat ttaaagaatt tcttgaagaa
    25321 attcaaaaaa caaatcccaa tcttcataaa atcaggggtc tggcatataa agaaaacaat
    25381 gatgtaatca taaacgaagg cagagagccg atagataatc tcgacataat taattacccc
    25441 tatgaggttg atgataaatt tgcaaataaa ataatatact atgaatcttc aaggggatgt
    25501 ccttttagct gcagcttctg catgtcatcc atcgataaaa aaatgagatt tttttcgatg
    25561 gatagagtta aaaaagattt aaagatgctt ttaaatacaa aggcaaggca gattaagttt
    25621 gtggacagaa cattcaatgc agatttcagg cgctccatgg agataatgca gtatattgta
    25681 gaaaataaca gaaataatat gacaatacat tttgaaataa ctgccgacat aataaatgat
    25741 gagtttttaa gctttatagc gaaaatgccg gttaatatgt ttcagtttga aattggtgtt
    25801 caatccttga atgaagatac tttgcgtgaa atcaacaggc atatggacaa ggataaatta
    25861 tgccgcgtta ttaaggaaat aggaaaaaac aaaaatgtac atatgcacct tgacttgatt
    25921 gcagggcttc cttatgaaga ttatgaaagt tttaaaaggt catttgacgg tatatataat
    25981 cttgatgcag agaaaattca gcttggtttt ttaaaggtgc tgaaaggaac caaaatatac
    26041 gaagacagag aaaagcacca aatcaaatac ggcttgaagg ctccatatga agtgatatgc
    26101 acaaaatata taagtttgaa ggaattatta aaattaaaaa atattgaaga attgctggat
    26161 aaatattata atgaaaaata cttcagcaaa tccttaaaat atataacatg ctatatattt
    26221 cccgattcag cttttgaatt ttacagctct ttcagtgatt actggcagaa atataattta
    26281 tataattatg ctcacagcag gaaagagctt tacaaaattt tgtatgacta tatagtacat
    26341 atgaaaagaa agaatgatga atttgttcaa gcattaaaat ttgattatat attcaacaac
    26401 caatatgaag agctgcctga ttatttaaac agagagactg aagataaatt taaaagcata
    26461 aagcggcagc tggcaaatga cattgaattc aaaaataaat atttcccttc aatgaaaggg
    26521 aaaattataa atgatttcag aattgcagaa atcggaggga aaatagtatt atttgtatat
    26581 agggacaggg agaatatttt caacagatgt gatacttatg ttgttaataa catagaggag
    26641 attgtttatg gaaagagata aatattttga agagtattat gaaaaaaaaa gaaataatat
    26701 ttcatttata accttaaaaa aaggtgcagc cgttaactat aaggataaaa catatgctgc
    26761 aaaaaaagag ctgcctgtac ctgtaagaat tgaaaagctt ttggaggata taaacaaaca
    26821 aaatgaaatg gacggaataa cactgaacaa tattattgac ggcataatat atatattagg
    26881 aactgacaga aacttcgaat atatacaaga ttataaggaa atgtttaaag aactcaattt
    26941 cgaattaatt ccttatatta tatattgcat aaacaatgac attaaaacag aagacggtgt
    27001 ggtatacgga agagctttga ttaacagtga agaaaatgaa aaaacctgct ttatttatgc
    27061 ttcatgtttg gaaaagatgg ggatggaata tcatgaaaaa ggaaatgatt caagccgata
    27121 ttttttggaa gaagctcatt tctattatga aaaatgcctt gactataatg aaaagttttc
    27181 attggcatac tataaattag gttattatta taaaaggaag cagcaatacg taaaagccga
    27241 gctcatttgg caaaaacatc aggaattgga tgataatgat ttaagaatag aagaaataag
    27301 aaacgaatta attcaattaa aaccatttgt tgattatgaa aagggctaca atcttgtatt
    27361 aaaagaaaga ccagacgaag ccttggactt attattgcct ttggtaaagg attttggcgg
    27421 atggtggaat ttgttgtttt tcataggtct tgcatacagg tcgaagagag aatacgatat
    27481 tgcagaaaca tactttgaga atgtattaaa aattgacagc actcaaaagg aaacattaaa
    27541 tgaattgggc ttgtgcaaaa tatgcacagg caaatatgtc gaagcagctg aattgttctc
    27601 aaaattgtta agcctggacc cgggcaactg tgaagtattt tgcaacaggg cagtagccta
    27661 tttgtataac aatcaacttg aaagagcaaa agaagatatt caaacagcgt taaaaatcaa
    27721 cccagaagat gctgtggcac tcggtattaa ggaagaattg gaaaaaaagt attgaaataa
    27781 gattagcaag taaaaaatgt gtcatatata ttgattaatt ttaataatta ttgcaatatt
    27841 tctcgaaaat ctcttaatat agcttgcaca agatatgaag attatgttat aatggttgta
    27901 caattgatga taaattatac agcgagtgtc attctaaagg cgaaacctga agaatttcat
    27961 gagggctcga tacataagag aacttaatat agaacttgga gattaatagt ggcaagaaaa
    28021 aattttggtt taaaaaattt ctttctttct tttgcattag ttgttatagc catttatgta
    28081 tttcaaaata taaaatctcc tgcagacttt atgatagcag aaaacggaat attagaggat
    28141 gttattaagg ctgaaggaat tgttgttaag gatgaaaaag tttacagtgc aacccttgat
    28201 ggcaacgttt tgtattatta tgaggaaggt acaaaaataa aacaaggaca gcttatagca
    28261 gacataaata cggatgcgga ttccgttgaa gtaaacagac aaattgccga aatacaagct
    28321 gccataaata aattggaaaa cagcagtcaa gttttaacta aagaaaattt cactgcttat
    28381 caaaacgaat tgcaaatcag tatactgaac aatgaccttc agaatatgta cggtatagtc
    28441 gaacaaatga ccaataatga tttttacggc agcaaatatg aagatttttc aatcagccag
    28501 ctgattgaaa tgaaaaatag cttggcaaac agtatttcta ctcataaggt tccatattat
    28561 tcagacaggg cgggaataat atcatataaa accgatggat tagaaggtat ttatagattt
    28621 gaaaatgtta tggacatgac cccaagcagc accctaagac gtgagtattc attgaaggat
    28681 aagagagagt ctgttcaaaa ggatgaagct ttctataaaa taataaggaa ttttgaatat
    28741 tatataattg taactataaa taatgaaaaa gcaagactgt ttgaagaaaa taaatacata
    28801 aaggttagaa tactttcaga tggggaacag cacatagtct ggggttatat agaaaaaata
    28861 aattacggca gcgaagaatc ggtattaata ctgtattttg atgactattt ctacaaggtt
    28921 tatgataaga gatacgttaa tatagagctg attacggcag catatgaagg gctgaaaata
    28981 aaaaacgaat cactgataga agttaacggt attaaaggtg tgtatgtaca agatgccagt
    29041 aatataatca aattttttcc cgttcaaata ttagggagca atgaggaata ctcggtagta
    29101 tttccgggtg actatatagg tataaatgaa aggaatataa taactatcgg agaaaaaaga
    29161 tatcctacaa ttaagatttt cgataaagta atacttgaac ctgataaggt gtatgaaggt
    29221 caaattgcag attaggaggc aaagttgagc atcagagaaa atattgacga tattgtaaag
    29281 agaattgaag atacctgtaa aaaagtcgga agaaacccga atgatataac agtgatagct
    29341 gtatcaaaga cggtagaatc agaaagagca agagaagctt ttgaggcagg aattaataac
    29401 cttggagaaa acagggttca ggaactggta aaaaagtacg acgagcttaa agatttggac
    29461 attaaatggc acatgatagg tcatttgcaa aagaataaag taaaatacat aatagataaa
    29521 acagtactta ttcattctgt tgaaagttta agtctggcgg aagaaataaa taaaagagca
    29581 gaaaaaaaca atttaattgc aaatgtactt gttgaactta atattggaga agaagacagt
    29641 aagtttggca taaaagaaga aagtgtttat gattttatac ttagtttgga gaagtttgaa
    29701 aatttaagag tagtcggatt gatgacggtt gccccgtttt gcgaaaaccc tgaggatgta
    29761 agatgggttt tcaaaaaaat gaaatatata tatgataaaa tttcaactat gaatttgaga
    29821 aatacaaaaa tgaaatattt gtccatgggc atgacaaatg actttgaaat tgctatcgag
    29881 gagggctcta acattattcg tatcggaaca gctatatttg gagccagaaa atataaggag
    29941 gaagagtaaa tgagtatgat ggaaaaagtg aaggacttca ttggcatcac agacttggaa
    30001 gaagactatg aggaggagga tcttgtttct gaaggaacag aaagaaacaa aaatgaaaga
    30061 atgggaacca acacaaaaag aaacaatgtg attaaggttc actccaacac tgatatgaaa
    30121 gtctttattt gtgagccgga aaaatatgag gattgcacaa aagctgttga tgaaattaaa
    30181 aacagaaaag ttgtagttct taatattgaa ggaatggaac ttgaggacca aaagcaagta
    30241 tttgaattta tcaaaggtgc aatttatgca ttggaagcaa gcattcaaaa agtatcaaac
    30301 ggtatattcg tactagctcc gaacaatgtg caaattgacg gaagattaag cgacagatat
    30361 gaaaggaatt attattacgg taaataagga gcatttatgt tttatattat acagttggct
    30421 ctagcatcac tattaagaat aatttattat ttaatactgg tcagagtgat attatcattt
    30481 ttcccaaccc tacaaacttc aagaatatcc tactttatct atcaaatgac cgagcctgta
    30541 ctggctcctt gcagggcaat tttagataaa ctgggattag gcatgggaat gtttgatttt
    30601 tctccaatat tggctcttct attattaaac atattacaaa gattgatata cgccatatag
    30661 atggctgcaa tactaccggg agaaaaatga aaagtttaga aaaatattat gattttatca
    30721 gggatgatga aatcaaaaac acggtaaaaa agatacaaga taaagcatcg tatgttaata
    30781 aaaactatat tgccgcaata accgaattta taaatccata tgttgtaaaa atcagtattc
    30841 cggtaataaa aagttatgat ataaaatttg aaattttccc ctcatatgaa aattgtgaga
    30901 ggaaagtttt cattttatac ccgcaatatt ctgaaataga tgaaaatgaa tttatttcgg
    30961 gaattcgtat tactaataag tctaaattca aaaaattaag ccataaggat tacttaggag
    31021 tattgatgtc cttaggtatt gaaagaagta agctcgggga tatctatgtc tgtgatgatt
    31081 acggggatgt agtggttcac tcggatattg ctgattatat aatttacaat ttggataaaa
    31141 taggtcataa taaaatagaa gcagaaaaaa ttaacttaag ccaagtcaat tttaaagaac
    31201 aggaacatga aatcttgaat attattacct cctccatgcg cattgacaat atcgtaaagc
    31261 accttacaaa attatcaagg gaaaaggcct ctttcataat aaaatcggga gatgttaaaa
    31321 tcaatcatat tcctgaatac aaggtaagca cagaattgaa ggaggaggac atcttgtcaa
    31381 tcagaagggc aggacggttt aaaatatata aaataatcgg tacaacaaaa agcggaaagc
    31441 taaaaattca aattaagcat tatatttaac gggagaagta atgagtatta tagcagtatt
    31501 tataattata ttttcagttg tttttgacca attatcaaag tattgggcaa cagaagtttt
    31561 aaaaaacggt gaatcaataa aaattatagg aaattttttg cgtttcactt atgcggaaaa
    31621 cagaggtgct gcattcagca tattgcagaa tcaaagggta ttctttttga ttattactat
    31681 tataatgctt attatattag gaatagttta ttttacaaac agaaaccttt caaagttgtc
    31741 cagattatcc atggctatga ttgcaggagg agcaataggc aattttattg acagatatcg
    31801 catgggatat gtaatagatt ttattgatgt aagatttgga actttttata attttccggt
    31861 atttaacata gctgacagct ttgttgtatg cggaacaata cttatggtaa ttttaattct
    31921 atttaacaag tttgaaacga gttgagaaaa tgaaaaagtt aaataattta aatagtacaa
    31981 cggaaaaaga tatagaaaat ttagacgata ttaaagaaat agatgatttt gacaattccg
    32041 atgatgatga ttttgaagac atggaaattt cagacagtga aaaagaaatt atccttgttt
    32101 cagacgaaga aaataaaaga attgacgtgt tcatttctga aaaatcaggc gaatcgcgaa
    32161 actttgtcca aaagcttatc agcgatggaa atataaccgt aaatgaaaga gaagttaaag
    32221 ctaattataa agttaaaata aacgatacta taaaaattat aataccgcct cctgaaattt
    32281 tggatgtgaa ggcagaaaac atagacttgg atattgttta tgaagataac gatttagcgg
    32341 ttataaataa acctcaggga atggttgttc atcctgcccc ggggcattat tcaggcacta
    32401 tggtaaacgg acttatgtat catctgaaaa atttgtcaac cattaacggg gttttacgcc
    32461 ccggtattgt tcacagatta gacaaaaata cttcaggttt gatcttggtt gctaaaaatg
    32521 acagatcaca caacttttta gccaagtgtt taaaggagca cacaattaac aggatttatt
    32581 atgcattggt taaaggaaat atcaagaaag atgaaggaga aataaacgca cccttgggga
    32641 gaagcgaaaa agacaggaaa aagtataccg taacaaataa gaacagcaag gaagcaatta
    32701 caaattattg ggtagtggaa aggtttggta aatatacttt attgaaactg aaattaaaaa
    32761 ccggcaggac tcatcagata agggttcaca tgaagcatat aggacatccc gtcgttggtg
    32821 atgatgtata tggaaatgta aatgaatttg gtttggaagg acagctcctg cattcgaaaa
    32881 ttttgggctt tattcatccc acaaccggca agtatatgga atttgaaagt gaattaccgg
    32941 attattttgt taaagtattg aataaaataa aatcttaagg gcattgcatg tcagcctgag
    33001 cgatagcgaa agatctcata ttgggaggga atttatgaaa ataaaagcat taataatgga
    33061 tgaaaaagcg attgaaagag caacattcag gattgcacat gaaattatcg agagaaataa
    33121 gggagttgaa aatatagtac taatcggaat aaaaacaaga ggatggccct tatctatcag
    33181 attggcgaaa aaaattgaag aaattgaagg ctccaaagtg cctgtttttc ctttggatat
    33241 tacttattat agagatgatg tcgaaaagga tgacagggct cccttgtcag taaaaagttt
    33301 tgactttgac attaagaata aaacagtggt attggttgac gacgttttat acacaggcag
    33361 gacagtaaga gccgcccttg atgccattgt ggaccaggga agacctaata acgtggaatt
    33421 agcagtatta attgacagag gtcacaggga acttccaatt cgtgcagact tcatcggtaa
    33481 aaacgtacct acttccaggt cggaaagaat aagcgtccgg cttgaagaaa cagataaaat
    33541 taaccaggtg gttataaccg aataagcggg acagtgggga cgtaccttgc tgtcccatac
    33601 ctgtgacagt caagatacgt ccccactgtc cctttttatc ccttctatat tcggcttaac
    33661 aagcattgaa taatttgtgt cgtaaattac aaagccgggc tttgcattag gtggcttttt
    33721 tatatttgat ttttttgtgt agtcgacttc aaccgtgctt gaatttttcc ccttgctgta
    33781 atatgctgca attttagctg cttcaatgaa ttcttcatcc gttagttcat cgcctttcgt
    33841 tcttatgata acatgggagc cgggcatatt tttggcatgg aaccaataat cttcattttt
    33901 agctatttta aatgttatca tatcattttg ggtattgttt tttccaacca gtatttcatg
    33961 cccgcctgaa gaaataaatg cagtaatagg ctttgtcttg tttgatttat cagccttaga
    34021 ttttcttttc ataaaacctt cggaaataag ctcgctgtat atgtcgttta aatcatcgat
    34081 tgtttcacat tcttcaatag aaaataatat attttccagg tattttattt catttaaagt
    34141 tatatcgatt aatttttcga gttcttcttc tgcttttttc agcttattat atcttttgaa
    34201 aaatttttga ctgttttgtg gcagattaaa tctggggtct aagggtattg taatttcctt
    34261 ttgttcaggg tcgtagtaat ttataaccct aagcttatgg ttttcaggtg ttttgtgaag
    34321 atttgcaaga ataagctctc cgtaaatttt gtacttatct ctgttttcgg cattaagaag
    34381 ttcatttctt tgcttttcaa ccttttttct gttcctgtct atttttgtat taatattttt
    34441 aataaggctt gcaactcttt ggttgatttt gtttttgttg tctaattcat aataatattc
    34501 atcaagcaat tcaccgggtg ataagaaatt tttcctttca taatctttta aatattcaac
    34561 ctccaaacaa taaaagccat attggttcat attttcatca taaaaaatat taaaagaaaa
    34621 atcattttgc tttattttat tcattatttt actaaacaca ttccatattt cttctttctt
    34681 ttcttcgctt aattcgccta aataagtgct ttcatttaaa ttggtcaaat aacaaatttc
    34741 tcttgaaata aaaggactaa gacctaaaaa gtttttatat aaaaaattat aaatgtatat
    34801 tccgttattt gtagactcga tttccttcat aaataaatct tttgtaacag tgagcggatt
    34861 taatttatcc gagccaggcg gaataacata tttcagcccc gggtagactt gccttacaga
    34921 gcttatattt tcggcaactc ttttaatggc atcaattata gtcttatctt cgtttataaa
    34981 tataatattg ctgtgttttc ccattatttc ggttatcagc gatttattca cggcagttcc
    35041 gaattcatct ttgcactcaa atgaaatttc cacaattctg tcaaaattaa tttgttttat
    35101 atttgttatg tgagcacttg ttaagtgttt tcttaacagc atgcaaaaca tgggaggctc
    35161 cgatggattt tgccttatta catttgtaag atgaagcctg gggctgttgc ttattgcagt
    35221 aattaacagt ttatagtttt ctcctttgct tcttatggat attactattt catttttatc
    35281 cggctgataa attttatcca ctcgtccgcc aattaacttt ttatataaat catttattat
    35341 actgtttaaa aaaataccgt ctaatgacat atttcctcct gcttgttact cgtgattaga
    35401 atatctaagt aagtatcttt ctttagttaa taaccatatt gtatattata aacattataa
    35461 tgtcatgaat tttttaaaat atttaatata aggctgccgg ttttttctga attattctat
    35521 catagctctt agactgcaat atttttgttt actaataagg ttttagtgtt ataatggtgt
    35581 aataaggtgt gggacacaca gataggtata tgataatact ctaaattgag taagtaggaa
    35641 aataattaaa ataattggag aataaaatgg caaatatatt tagtatgacc ggttacggca
    35701 aaggtgaaca caatgacggt aaaagaagta ttacagctga aataaaagct atcaacaaca
    35761 ggtattgcga tataaatata aaaaccccca gacatttgcg tatttatgag gataatataa
    35821 gaaaaatctt gaaaaacggc attcaaaggg gtagaataga tgtatatatt aacattgaat
    35881 atataagtga atcagataca gtaattttac cgaatttggc cctggctaaa cagtatatgg
    35941 atgccattga aaaaattaaa tcagaattag gaattaacgg caacccatct cttgacacaa
    36001 taatcaaatt tcaggatgtt cttattgcaa aggaagatga tgccgatgat gatgaactcc
    36061 gtatttgtct ggaaaatgca atgaatgagg ctgtaaaaaa cctgctgaca atgagagtta
    36121 aagaaggtat acagttaaag gatgacatta gcctaagcct ggtaaaaata cgtgaattaa
    36181 tggaagtcat atctgaaaac tcccatacaa ttgtggagga ttataaaatt aaatttgaag
    36241 acaggattaa agagctgttg ggaaatactc acgagcttga tgaaaacagg ctttacaatg
    36301 aaattgtaat ttatgccgat aaaagcgata ttaatgaaga aattgtaaga tttaactcgc
    36361 atatggtgca attgtccgat gctttggaca gcggaggtgc aataggaagg aagcttgatt
    36421 ttatcttaca agaagctaac agagaaatta ataccatagg ctctaaagtc agcagcttag
    36481 atattataca agttgttatt gaaataaaga atcttttgga aaaaatcaga gagcaagtac
    36541 aaaatattga ataggagtta aatatgtcaa atactattaa tattggtttt ggtaatattg
    36601 cgctggcaaa cagaattgtg gcaattgtaa gtcctgaatc ggcaccgata aaacgcctta
    36661 ttcaggaaac ccgtgaaaaa aataaactta tagatgcaac ttacggcaga aaaacaagag
    36721 ctgtaattat tactgattgc gattatataa ttctatcggc aattcaaccg gatacaatgg
    36781 cactgagatt tgagaaccac aatatcaagt agaggtgagt tatggatgaa ggattattag
    36841 tggtaatatc aggcccttcg ggagcaggca aaggcacaat ttgtgctcag cttctgttgg
    36901 aaatgagtta cctgaaagtt tccgtatctg ttacgaccag aaagccaagg gcaggcgagg
    36961 tagacggaat aagctattat tttattgaag aagaagaatt cataaataga ataaataatg
    37021 acgaatttct tgaatacgca aaagtttacg gcaattatta cggtacgccc aaagaagaag
    37081 tgtttaagca gcttaaagcc gggaatgata taatattaga aatagatatt caaggagcac
    37141 tacaggttaa aaagaattat ccgatgggag tgttcatatt tatccttccc ccgtcactgg
    37201 aagaattaaa atccagaatt gaaggaagag gtactgattc aaaagaagtg attctaagaa
    37261 gaatggagtc agcttatgag gaattaaact atgctttcca atatgattat gtggtggtga
    37321 atgaccatgt ggatactgca acagaaaaaa ttaaacatat aatattggca gaaaaaaaca
    37381 gagtaataag gaaaaaagaa ataatcagta aaatcaggga gataaagaat gaaaaaaata
    37441 tcaatagata aattaaatga tatggttgac agccagtatt cattagtgac aataatatca
    37501 aaaagggcaa gacagataat tgacggctcg gagcctttaa ttaaaacaaa gactaaaaag
    37561 cccgtgtcaa tcgctattga ggaattctat gaaagaaaat ttgaagccat atacaattat
    37621 gacaagtaca aaaaatcatt gacggcaaat aaggagacag ctgttgaaac aacagatgaa
    37681 aaaaaagaca tggatgaaac aacggcacag gaatagtcag ataatcagtc aaaggactga
    37741 ttatttaatt ttagttgagc ggagaaaata tgtataatac ttttgtacaa gttattttaa
    37801 ataatatttc caaaagcaca gacaaaattt acacatacgg agtaccggat gcattgaaaa
    37861 gctttgttga tgaaggaaaa agggtcagaa ttaatttcgg cagagggaaa aatattattg
    37921 atgcattaat agtatccgtt gatttaacct gtgattatcc cattgaaaaa attaagccta
    37981 ttttagatat aatagatgaa aaacctattg taacaaaaga aatgattaaa atcctttttt
    38041 ggataaggga aaggtattta tgtaagtatt ccgatgccat gagattgatg ataccttccg
    38101 aaacaaaaag tatttacgaa aaacatatca gactgaaaga ggataaggaa atatcccaat
    38161 acaagattaa caaaaccgaa aaccaaataa aagttataga atatcttaaa aatcacaaat
    38221 ccgccagcat agctcaatta atggacaggc ttaatatttc taaggcagtg ataaataact
    38281 taataaaaag agatataata gaagaaagtt tggtttttga ttattcaaac caaagagagt
    38341 ttcaatcgga tgagccctta tgcttaaatg aagaacagaa gaatgcatgc gatacaattc
    38401 ttaactcaga caaaaaagta tttctcttgc acggtgtaac aggcagcggt aagacagaag
    38461 tctatatgag cataatagaa aaatatttaa aagaaggcag acagtcaata atgctcgttc
    38521 ctgaaatttc tcttacggcg cagacaattg aaagatttga aaaaaggttt ggaaacaaaa
    38581 ttgcagtatt tcattcaaaa ttaagtaaaa aagacaggta tatacaatgg atgagggttt
    38641 acaaaaaaga ggcagatata gtaattggag cacgctctgc cttatttgcc ccccttaata
    38701 gattaggtgt catagtaatt gatgaagagc atgaggacag ctatatttca acaacatctc
    38761 caaagtatga caccatggaa gttgccgtaa agatggcgta tatgatggac ggcaaggtga
    38821 tattaggctc cgcgacacca tctttaaata cctactacat ggcaatgaaa ggccaaatag
    38881 aattaattga aatgaaaaac agggttaaag atgccgaaat gcctgaaatg aaaattatag
    38941 acatgtcagc tgagcttgac aagggcaaca ataccatagt cagtgaagat ttatacgaaa
    39001 gtataaaaaa ttctctaaag caaaatgagc aggtaatact atttttaaac aagagaggtt
    39061 attccggatt cgtatcctgc aaaaaatgcg gttatgtagt gaggtgtcag aggtgcgatg
    39121 tttccatgac ctatcatgtt gtaggaaata taatgaagtg tcactactgc ggaagtgcaa
    39181 aaaaaatgat ttacacctgt ccaaagtgtg gcagcgaaaa aattgagcag ttcaatgcag
    39241 gaacacagca tgttgaaaaa ctgataaatc aattatttcc atatgcatcc acagaaagaa
    39301 tggacttgga ttcaatgact gacacagaaa gctatgaaaa tatatataat gattttaaga
    39361 ataagaaaat tgatatatta ataggaaccc agatgcttgc gaaggggttt gacttcccga
    39421 atgtaactac ggtaggagtt ctttcggcag acgcgatact aaatttacca ttttataatg
    39481 caagcgaaaa aacatttcag cttataacac aggtaagcgg aagagccggc aggggagaca
    39541 agagaggaaa tgtgtacatt caaacatacg agcctgaaaa tttcataata aatgcagcaa
    39601 aaaacaatga ctatgagcta tttttgcatg aagaattaaa attgcgaaaa gaatttgcat
    39661 ttccgccatt tattaatata ataaatatat gtttgatatc aaaaatagag gaattggttg
    39721 taaaaacagc caatgaaaaa tacttggaag taaaagaaac cgttaaagaa atggtaaagg
    39781 aaagaagtct attgttgtat agaccggttc ctcacaatat atacaaagtt aatgatgagt
    39841 accgaattaa tttatatata aaggtgtcaa accatagctt gtcggaactc aaaaaaatac
    39901 taaggactgt atatatggag aaggatatag aaaatataaa ggtaagcata aatatcaaca
    39961 cggatacaat ttaggagggt tcaaaaatgg cattaagaaa tttacgatat gaaggcgatg
    40021 aaatattaag aaaaaaaagc agggaagttc cccttgttga tgatagaata agaatgctaa
    40081 tggaagatat ggtcgaaacc atgtataaat atgatggtgt cggacttgca gctccacagg
    40141 tagggatatt aaaacgtgta atagtaattg atgtggaaga cggcaaggta tataaaatgg
    40201 ctaatcctaa tattattaac gaatcagggg agcaatcagc tcaggaagga tgcttaagcg
    40261 tccccgagaa aaagggcata gtttctagac ccatgaaagt gattgttgag tatacagatg
    40321 agaataatga aacagtcaaa ctggaagcgg aaggtttatt ggcaagagca atatgccatg
    40381 aaatagacca tttaaacgga gtattgttca tagacagagt aaccgatgat gtaattgaag
    40441 aataggtgat gttatgaatg taatatttat gggtacaccg ggttttgcag ttccaacctt
    40501 agaaaaacta catgaaagca aacacaatct tgtcttggtt gtaactcaac cggataaacc
    40561 gtcaggcaga gggaaaaaat taaaaaaatc agaagtgaag gaaacggctg aaaagctagg
    40621 tcttaatatt tttcagcctg ataaaataaa aaaacaggaa aatattgatt tattaaaatc
    40681 atataatcct gatgtaatag ttgttgttgc ttacggacaa atactgaaca aggaaatttt
    40741 gacattgccg aagtacggct gcatcaacgt ccatgcttca ctgctgccga aattaagagg
    40801 tgcggcacct ttaaactggg ctcttataaa cggagaaacc aaaacaggca taactacaat
    40861 gcaaatggat gtgggcttag atacgggtga catgctttta aaatcagaag ttgaaatcga
    40921 tgaaaatatt aatgttggtg aacttcatga tattttaatg cataaagggg cagaactctt
    40981 gatagaaaca ctggataaat tggagaaaaa tgaattaaca ccgcaaaagc aggatgattc
    41041 tctttcaagc tatgctccga tactaaacaa cgaaaaccgg aaaataaatt ggaatttacc
    41101 tgcaaagagt atccataact taataagagg attatctcct tggcctgcag catattttac
    41161 tctggatgaa aaaaccgtaa aagtgtacag ctcatcgtat ataaataatg attcagatta
    41221 tgagcccggt tatgtaataa aagcaaataa tgagggaatc tttgttaaag ccaaagatgg
    41281 tatcgtaatt cttaaagaaa ttcaaatgcc cggcaaaaag aaaatgccgg ttgaagcata
    41341 tttaagagga aataaattcc ccgaaaaaat catcttatga caaggggacg ggggtactgt
    41401 catcaaatga cgaggggaca ggacaagtca atgtagggac gagttactga tgccccaaaa
    41461 taatataatg aattggtgat attatgaata ttaaaaaaac tattttattg ttgaaaaaag
    41521 taccgttatg ggttattgta gcagccgtgc ttattttagc cgtcatagct atagctattg
    41581 gcacaaaata aatgaaagat aagtagaaag tttgactgct aaattcttta ttaatataaa
    41641 aaatactagt gaggtaatta tgaaatttac atttaatcat tttaatttta atgtccttga
    41701 tttagagaaa agcatgaagt tttatgaaga agcattaggt ttaaaagagg taaaaagaaa
    41761 agaagcagaa gacggcagct tcgtaatagt atatttaggt gatggaaaaa caaacttttc
    41821 attagagttg acttggatga aaggaagaac agagccttat gatttaggag aacaggaatt
    41881 tcatcttgca atggctgcgg acaattatga cgaagctttc aaaaaacatg aacaaatggg
    41941 ctgtattgaa tacataaacg aaaaaatggg tatatacttt atcgtagacc cggatgggta
    42001 ttgggtagaa gtgataagag gataatttat caaaaattac catgaaacaa aattaaaaaa
    42061 ataacgtcta atttatcaga agtaaaaatc ccataggccg tggtacttca aaataaatta
    42121 caagaggtta tttatgattg aaagaaaatt attaaaaata ttatcattaa gtttagtttt
    42181 atcattatca ttaattggat ttgtttttgc agaaactaaa gaaaacttgg atgagccggt
    42241 ttctatacaa ataactgcgg ttgatgaaga aatgtatgag gaacaagctg atattgacaa
    42301 attattattt gagcaaaact atgctaatga cttagaatta aagggtatat atattactca
    42361 tacaagcact gttgatgatt atgttgaaat tggaatcaca ccatatacac cggaaaatgc
    42421 cgatgccgtt gttagactgt taggaaaaga taatgttaag gtagttgaag gtgtattagc
    42481 agaaatactt caatacagta ctgataatcc cgaaactata gatccggctg aattagcaga
    42541 tgattcgcaa atagtcgatg taactgctga tgctgaggat ataagccagc cggcagatga
    42601 agatgtagtt gaggatgcag aaataataag tgctcctgct gaaaaggaag ttgaaaagaa
    42661 tggaatatca ccattcaaca tattagggct aagtgcattt gtaataggaa tcgtcgtagt
    42721 gctattcaag aaaaaaagtg cataaactaa agaaagctaa acagacgaca ggtgaataac
    42781 ctgtcgtttt ttcatagcat atgtaagttt aaaagcatga taacctgtat tgtatggaac
    42841 aatattagag aaaaagcgtc tttttaaata tatagttatg gttgatttta atcactgtga
    42901 gttgtaaaat gaaagcgtta gttattcaga atgaagtata tggaggttat tatggctgag
    42961 cctgtaaaag ctaatatcac tttagatttg cttgataaaa ttgatattag ggtaggaatg
    43021 ataatatcgg tagaagatgt tgcaaaatca gataaattgg taaagttaac agtcgatttc
    43081 ggtgaattca cacgaaacat attggtcggg atgaagaagg aaagagataa tcctaaggaa
    43141 attgaaggca aacaggcatt attcgtggtt aatttggctc caaagaaaat ggccggtgaa
    43201 atttctgaag gaatgctctt tgatattgga tatgccgacg gtataactcc tgttttggcg
    43261 cagccggaaa aaccggtccc taacggttcg agagccggtt aatctaatcc attaccggat
    43321 tattgttgct taaggaggca gaacatgaaa gcagggataa caagtcgcaa aggcaataag
    43381 atatttttta tcttttgata aggttttatt aatgctattt caacttgctt ttcttgcc
//
LOCUS       DAOSHP010000005        39784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_776_length_39784_cov_9.868610, whole genome shotgun sequence.
ACCESSION   DAOSHP010000005 DAOSHP010000000
VERSION     DAOSHP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 39784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39784
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_39784_cov_9.868610"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..703)
                     /locus_tag="PLB33_01115"
     CDS             complement(62..703)
                     /locus_tag="PLB33_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOG62150.1"
                     /translation="MIDTDKIVKIYDEDSKLIGKGQLLSLNEKIIKVKGTGLPILSRK
                     TNVIIEIYSEFVGISRYCCQIDLASDNQLNAHIVKKKPDIERRNSLKVRTDLSYYIES
                     LYRNDKDITKDFPNMKINLINLSIGGMLISSNYELYINDIIAFYFRCEKTAVLLKAKV
                     MRIDKIYDDNQKYLSFYNYGCIFEEMPPNIEMIITRYLYIRQLEMHKDKWEKL"
     gene            complement(726..1007)
                     /locus_tag="PLB33_01120"
     CDS             complement(726..1007)
                     /locus_tag="PLB33_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOG62151.1"
                     /translation="MSQYNNKSNHAVALKYNAAKNNAPVVIASGSGYIADKVVEIAEK
                     NGVPVYRDDSLSAILSQLKIGSEIPEELFSSIVDIYVYFLNFKMPESEA"
     gene            complement(994..2409)
                     /locus_tag="PLB33_01125"
     CDS             complement(994..2409)
                     /locus_tag="PLB33_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62152.1"
                     /translation="MNNISVKTNNHISENPINKPKIKNEVPFDIADPTKVTKTPEKGY
                     ENQIRQELFNYNPDSVFDKFIKSLQNSPVLYEEVKKLLLSKQFINNNIKNDPVLSVFF
                     DSFLGNIKMNEAEILDFLKFQQGTYTKFQGEFFDLLRTLLGNNPDNKGLQTVLKNFLR
                     SYDCFVSVKEIYGSINTTFKNIEKNIPEMLKASFNELTEKFIYDNTNAMDSNLELLKN
                     EILPFIGRYISKMNDFGIIRDYVSVLVHNLVRLEYGSKTNFSDELENLFEFLKYNFQL
                     DGKQMELLKTTLINTYETKSNIKNDSIESFFKLLDKGISESENAVTKGLMGDMTESIL
                     FSQNVNIPLLHMFLPLNYKGMFMFSEIWIGKDEDSPDNKRNLENHKSYKVFITFDIQN
                     LGYFETTLKLRDSVLSLDIYIPGSLSEQTEKIKSDLNFLLSKNNVTIESIRVQECIKA
                     RRFSEVFTNLTERKNGVDVTI"
     gene            2635..>3260
                     /locus_tag="PLB33_01130"
     CDS             2635..>3260
                     /locus_tag="PLB33_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOG62153.1"
                     /translation="MDNNLDSVLDMYLFETNSLLELLDNILLKAESNLSFDKECMDEI
                     FRAMHTIKGSSAMMQFDSIMTISHKIEDLFYYIRENGIDQKYNEELVNLVFKITDFIK
                     GEIVKVEEGLELEKNLDPLENEIKLFLNSIKKETKEEIYQIENLKSNIAITEENNQTK
                     GCTYAIRIIFDDDVSMENLRAFIIVNQLRETGIEFEYKPENIENIPETS"
     assembly_gap    3261..3360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3361..4431
                     /locus_tag="PLB33_01135"
     CDS             <3361..4431
                     /locus_tag="PLB33_01135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOG62154.1"
                     /translation="ESFKKSTRQVRKLTDELQDIVMSLRMVPISGTFNKMHRVVRDMG
                     KELNKDVELVLNGEDTEIDKTIADCISDPIMHLVRNAMDHGIESEEKRISAGKNKKGK
                     ITLSAFNTGGEIHITVEDDGRGFDSEKILEKAEAKGLLTKPASEYTQKEAFQFLMLPG
                     FSTNDDITEYSGRGVGMDVVKSNVEKVGGTIVLESNEGLGSKITFKIPLTLTIVSGMQ
                     VVVGNSEFIIPIKSIHQSFKTKKNEIIHNPEQGEMILIREKCYRIIRIHEYFNINTDI
                     TNIEDGILIFVESGDNSACLFVDKIIGEQQVVVKPLPIILNKYNLKESGIYGCSILGD
                     GSINLILDVGNLMESNRYGENN"
     gene            4415..5218
                     /locus_tag="PLB33_01140"
     CDS             4415..5218
                     /locus_tag="PLB33_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOG62155.1"
                     /translation="MVRITDNEFLEYVKYINKNYGIDLSKKKILIEGRLSNLIEKKGM
                     NSFYQYLRSVKNNDVEETMFVNKLTTNYTYFYREEKHFQYLTETILPYEEKNNKAKIL
                     NIWSAGCSSGEEPYTIAMILDDYFKFRINQWKIRIYATDISENVLSKAREGIYSKDSI
                     CKLPDSFIKRYFIKLDDNKYQVVPEIKNYVRFKASNLMAPILEKNRYHVIFMRNVMIY
                     FDLETKNKIINKLYNATRSGGYLMIGHAEIIQREQSKYLYIKPAIYKKE"
     gene            5220..5795
                     /locus_tag="PLB33_01145"
     CDS             5220..5795
                     /locus_tag="PLB33_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HOG62156.1"
                     /translation="MLINKIIVIGASTGGTEAIIKILKELPEDFPPILIAQHMPIGFT
                     KMYADRLNKICKMEVREALDGDRIKQGVVLIAPGDKHMVLKKDDKGYYVSCKKGDKIN
                     GYCPSIDLLFNSAAKIAGNKSVGIILTGMGKDGAEGLLNMKKAGAYTIGQDESTSAVY
                     GMPMAAFNIGAVTVQAKLEDIFEILTKHLTI"
     gene            5862..6236
                     /gene="fliS"
                     /locus_tag="PLB33_01150"
     CDS             5862..6236
                     /gene="fliS"
                     /locus_tag="PLB33_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOG62157.1"
                     /translation="MLNSLEQYKKTSVNTMTKGELLILLFDEAIKKLNQSKLLMEHED
                     YANANIFLTKVRHIFYYLTATLDGKYQISQELSDIYEFFNREIMKASTLKSTKHIDDI
                     LPIIKDLRDTWAEADILSRKKY"
     gene            6246..6605
                     /locus_tag="PLB33_01155"
     CDS             6246..6605
                     /locus_tag="PLB33_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62158.1"
                     /translation="MEELLRNKLEAAKELKKLTSFVNELSLIIDYNRVNSLLDERQQY
                     IDKINVINEKISEVKSKENYVETNEIKKLNKDIGRVFTEIYEIDKVIRKNINTELKSV
                     KEKLNYSETNIVVNIKI"
     gene            6643..8637
                     /locus_tag="PLB33_01160"
     CDS             6643..8637
                     /locus_tag="PLB33_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOG62159.1"
                     /translation="MKTNKSISKKITTTILIVCFSALLITGIIIVKTVSTRFKDNKRQ
                     ILNETALSISNKAEVFFEKYITVVEQMAHDKNLQEFMINAEGYQTLPVTRGFDIATAT
                     TDDIQKADSEIILTAYIAEDDYYLASPTIYTQSEYDVTSKEYYNAVTENKVCITDPYI
                     DAITGGIVITISAPVNVDGEIVGLTAVDIAIDKLINMVDEYELGDTGYFTLLSKNNVI
                     IAHKDKDNLLKKVDEINLSDNIVQSVINKDRSVVEFTYNNDGFIGNSVEIGDTGWQIM
                     SSLPKEEFMLNTNQLVLIIIAVFVILLVVLIGVLYVLINKLTKPITDITNITNKLAEG
                     ELDVNIDINSDDEIGVLANSIRKLTERLKLYIIYIDESVGVIDKMAEGHLNMELRNDY
                     AGEFAKLKKSLLNMSNTLKDAIGKIQQSSIAINMNAEQVSTGAQTLAQGTTEQASAIE
                     ELSAEINEIYTTIVNNAEYAENAGSKAMQASKEVERGNMQMREMLSAMDEISNSSSEI
                     GKIIKVIDDIAFQTNILALNAAVEAARAGAAGKGFAVVADEVRNLAGKSAEAAKQTTA
                     LIENSINAINKGTMLAGEAGKSLAGIVDKTNETNELINEIVKASSQQTISVNQIRRGI
                     EQISSVVQENAATAEASAANSEELSGQVLILNDLVKQFTL"
     gene            8793..10688
                     /gene="fliD"
                     /locus_tag="PLB33_01165"
     CDS             8793..10688
                     /gene="fliD"
                     /locus_tag="PLB33_01165"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOG62160.1"
                     /translation="MSILSTNLYGSSVNKGMGGLMSGLDTDDLVNQMTAATRNKINKQ
                     YQAKQKLLYKQEAYREISSKLLAFNNKYFSYSSGSKTNILSPKFFESYTYKSSSNYVN
                     VTGSADVISNFKINSITSVAKAAIYTTNSVSSQSFSSDEIIGNISEDVLLSSSITFDF
                     NGVKKTIALSEMVDGKPDGSPYTYDAEGLADFLNTKLAEVYGAGRVNAELDASQTSIM
                     FSASGSTDVFSVSNISNDLSILTGIKSGDSNRLKISKTIGDIYGEVTDCSIIINGKSI
                     DGISKDMSISEVLKKINKESDLDITYSNTTDKFVIVSKDTGKASKIEITGALADKLFG
                     GSIGSDIEIRGEDTVMNVTIHGQDIDITRSTANFSIDGINIELSKNAILAPEEDPITF
                     DVINNTDEVIERVKQFIDDYNEIIDLLGTKTKEKPRRDYPPLTPEQQDEMKEKEIENW
                     LKEAKKGVLFGDKNITSLLNKMRASMSGMTSVSDLALSNIGISAASMDTSGKLVFNEE
                     KFREKLIEAPDQIASLFTGIADETDEYAKSGIANQILDILRENIGAMGTSGKLIEEAG
                     LDTGRSSDQNNISLKIKDYDEKMKELKKSLERERQRYWNQFSALEQSLNKLNAQSSWL
                     MDMMGGY"
     gene            10707..11483
                     /gene="rfbF"
                     /locus_tag="PLB33_01170"
     CDS             10707..11483
                     /gene="rfbF"
                     /locus_tag="PLB33_01170"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HOG62161.1"
                     /translation="MKIVILAGGFGTRISEESHLKPKPMIEIGDAPILWHIMKYYSCF
                     GFNDFIICCGYKGYVIKEYFADYYLHRSDITFDFSNGNEMIIHNNVAEPWRVTVVDTG
                     LYTQTGGRIKRIKKYIGNETFMMTYGDGVSNIDIHKLVEQHKSTNNIVTLSAIQPGGR
                     FGVLDIDENDRKVIGFREKATEDAGWINAGFMVMEPAVFNYINEDKTIFEREPLENLS
                     AEGKLGVYKHYGFWQCMDTQRDKYHLESMWGKNMAPWKVW"
     gene            11486..12424
                     /locus_tag="PLB33_01175"
     CDS             11486..12424
                     /locus_tag="PLB33_01175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62162.1"
                     /translation="MKISVIMLTYNREELLTRAIDSILNQTFKDFEFIIVDNGSTDRS
                     GAIADEYALKDSRIKIIHREQGNIGSGRNAGLDAATGEYVAFIDDDDYAESDFLEFLY
                     NLAKKHNADIAVCGSTKEENGQQLPNGIYQYDEKYIMDAEQATVNFLWRKLYNAAMPT
                     KLVRREMFDSIRFLNEGKYDDITTTYRYFANAKIVAAHGLPRYCFYRHSGNNSSAATK
                     HHLLNPVQLNEYLAAFRERTEYISKILPQLAGLALYSEWSYMISMVEKIHRYGLNNCA
                     DLLVLMCDNLRAHWDDFYYGKYILEFEKVWMERYVK"
     gene            12447..13562
                     /locus_tag="PLB33_01180"
     CDS             12447..13562
                     /locus_tag="PLB33_01180"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOG62163.1"
                     /translation="MNNINFSRALEKSCCTGCAACANVCPNNCIFMEYDDEGFLYPEI
                     DIKKCTECGLCEKSCPAINNPVNDNFIEPEVYAAWSLDDEVRASSSSGGIFTELAHKV
                     LDNGGFVSGARYKTNNLVEHCLINSREELHELRQSKYVQSEIGLIFRDIKKLLEEDGT
                     VMFTGTPCQSAGLLQFLNKKNDNLLLCDIVCHGVNSPMVYLKYFNELEEEYKSKIKKI
                     NFRDKRNGWKDFGTSVVFENGKEYFCSQKREPFYRGFIKNLYLRPSCYNCQYKSFPRL
                     SDITLADFWGINFIAENEIYKGVSLLLINSNKGKHYLEEISKYISKEQKTLKDATLKN
                     PCILNSVTLNNKRELLFKALRKETDVVDIIQKIIDYD"
     gene            13575..14387
                     /locus_tag="PLB33_01185"
     CDS             13575..14387
                     /locus_tag="PLB33_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG62164.1"
                     /translation="MNKRVELYHSGLTITQKCTLKCKLCAGYSPYYNPQPHYSINRLS
                     EIIKRYFSIVDHVEKFTITGGEPFLHEDLDKILIETIKYRKQFGKIEIITNGTLFPNE
                     KLKAVLEKYAVEDGCITLLVDHYGELSKKIHELGLYFIEKGINHKIRIYYGENAHCGG
                     WVDFSDFSKKLFSVSQMMEKFKKCAIPSKLNYCLAIKDGEMHPCTFSHRIMELNLIER
                     NKDEYIDLFDDNTIEQQKEKIMKLFDIKYLSACAYCNGMCDDSVRFVPAEQL"
     gene            14397..15431
                     /locus_tag="PLB33_01190"
     CDS             14397..15431
                     /locus_tag="PLB33_01190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62165.1"
                     /translation="MVYIFTTAYNAEKTLHKAVNSILNQTYTDFCYYLFDNGSLDDTR
                     KIIKEYANKDNRIIPLFGESNCRRVIPKIIKDIISGDNMLKNSYFATLDADDEYSPDF
                     LEKMIDFVEDNNLEVATCGTEWIDEKTGQTIKKKVLNKNLILEGKEFADKFPLYRNFI
                     VTLWGGIYSLELLKKCDFEWYHNAMNFSDTAFCMETFRRAKRAGVLSESLHKYYISPR
                     TVSYKYNPDWFYACKYLHKISREYLLDYGEISKQNENYLCVIFLILIKYILPKIQQAD
                     VKLPEKLKSLHNIFTDDMTKYVLEHWDEVGIYSNKLDFLREIESWLYTQDGWGLLRKT
                     VEEIITAMNI"
     gene            15468..16775
                     /locus_tag="PLB33_01195"
     CDS             15468..16775
                     /locus_tag="PLB33_01195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62166.1"
                     /translation="MSKIIIYTPAYNAEKTLRRAVDSILNQTHKEFIYYLLDNASTDG
                     TYEIIKEYAKNDKRIIPLHNEINWVGNGIYEVLKNYGKDCYFCALDADDEYTPNFLEK
                     MLFFIKNENLDIAACGSDFVDMQTNKHLGMRLLKKNLILDEEKFDRYFPTYHQFMRTI
                     WGKVYSISVLRRPEYKRYKQPGYGNDTLFAMNAFYNANRVGILSESLHKYYVSPKSVS
                     YQFDKNRTKSDRVLFDAACDFLISKCGAVSDENLNYLWIVYFKAIKDTLNVLLNAQIS
                     VSDKFEYLLDIFQSRHTQELIKWPGAKAEKNRLFSQAASRMLSSSPYLVGLEVEFLCY
                     FKDIVFSILQNDEENALSQIIELIAEGTEIPDEYVESLMLLALNLSAKLELHDYFIYF
                     KKLQISLLIELSKTNEAIKEFADWDEILSDDIDFKNLKERLGI"
     gene            16772..17887
                     /locus_tag="PLB33_01200"
     CDS             16772..17887
                     /locus_tag="PLB33_01200"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG62167.1"
                     /translation="MKKYLLYGHGGAYNHGAEAIVKSTVDVIKKRHPDSKVILSTHFK
                     EQDIEFNMPIDEYCERDMKYVELDKNSSEKGIYDRFIYKSTLEQITEDTVCLSVGGDN
                     YCYDNWRKWKAIHERAIEEGARSILWSCSIEPSMMDNEMLDVLKSHHLITARESITYN
                     ALKDRGLDNIRLCSDVAFLLEPKEYSLPENFFVGNTVGINISPLLIRREKTEGIVIKN
                     VIKLINLVINKTDMNIALIPHVLMPMDNDLEILGKIFNRFKDTGRICFVNKNISAAEY
                     KYVISNCRLGVFARTHASIAAYSTFVPSMVIGYSVKSKGIAKDLGLEDFVLPIEEFHN
                     DCCMVSMFEELMNNEQNIKKILADKIPLYRKRAMPNY"
     gene            17919..19211
                     /locus_tag="PLB33_01205"
     CDS             17919..19211
                     /locus_tag="PLB33_01205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG62168.1"
                     /translation="MYITQCLIAKNEEANIEHCLSHLKTVVDEQIVIDTGSTDRTVEL
                     AEIMGAKVFHFDWINDFSAARNFALDKAKGDWIIFLDCDEYFSEDSVPLIRKCIKKFG
                     RQKEIEGLTTNFINIKSNGEIISAVKNVSARIFKRKSYLRYKNKIHEVLVNVNKTDDN
                     IVRADISDLLKIYHTGYDKTIVNEKNKNERNFQLLERELKNNPKDIKINYYMSRQMNI
                     EGKYEKAIEYGLKAISYIDDFYEFDYYCTIYSGIMINMLSLLKSYDEIKVIFDDAIKK
                     YPGYPDYYWLLGIAAVRGNKYEEAVNLLEKCIKLCEKYNCNAESLALGKIDDVYIELL
                     NAYNHQNNKHKIVKTAVSILKVNKYNIQVLIILLNTFLTQEKEENIVAFLNKIYDYNL
                     FKDKIYLFKASKIVDNIILVEFFRKLFNDEELKKINNI"
     gene            19365..20192
                     /locus_tag="PLB33_01210"
     CDS             19365..20192
                     /locus_tag="PLB33_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017695304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOG62169.1"
                     /translation="MRIQHNLNAMNSLRQLGTNNTNTGKNLEKLSSGFRINRAGDDAA
                     GLAISEKMRAQINGLEMATKNAEDGISLIQTAEGGLNETHAILQRMRELAVQAANGTN
                     QAEDLQSIQDEIDALADEIDRIGDSTKFNGIDLLNGGSMASDGIDFQIGANNADYESV
                     NLTIASMKASDLGVDTIDVTATGDPLGAIDAIDAAIADVSAARAGLGAMQNRLEHTIN
                     NLGVTTENLTAAESRIRDVDMAKEMMAYTKNNILTQAAQAMLAQANMQPQGVLQLLQ"
     gene            20400..21068
                     /locus_tag="PLB33_01215"
     CDS             20400..21068
                     /locus_tag="PLB33_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG62170.1"
                     /translation="MRILIVEDEVKITQALSYLFNKQKIDVDIANDGEEGLILASKDI
                     YDVIVLDIMLPGVDGIQILKTIRKDGIKTPVIMLTAKDTVDDRVFGLESGADDYLVKP
                     FATKELIARVKALARRKSTEFVGEIYEFEDIKYDVKNYLLYVGDNEYKIPLKEAMLME
                     MFIKKPRQVFTREQIIDRIWGLEADILESNIEIYVHHLRKRLENTNVKIETVRGVGYM
                     LKEK"
     gene            21068..22333
                     /locus_tag="PLB33_01220"
     CDS             21068..22333
                     /locus_tag="PLB33_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG62171.1"
                     /translation="MFRKLHLKLTAYMGIILILFMFFVSAGIYQFTKMVFEDGNKELM
                     RAEALRIYNYKKTAYTQYFFNGTLFKRYIQINPSENRRLDACYVVYDQYMIPVLSEEG
                     EIKIADHIKPLAQKAFEEKKDSYETQKIGRFNYRIYTRYFSDYTGQSVVQVYQNTVNE
                     DILWSFLRTVLIMIGITGTVILLVLSYMLTGKALAPIKETWKRQREFVADASHELRTP
                     LTVIQTNLDVVLSDEDGTVDENEIWLDNAYSETRVMARLIDQLLILAKADNNEEELDI
                     SEFSFSEVIENVCQSMEVIAAKKNIELITNIEDNIIIKADYDKMRRLAVILIDNAVKY
                     TEKGKVTVSLKTDKNKKVLIVEDTGIGINEKDLERIFDRFYRADKSRHREGGVGLGLS
                     IAKWIADKHKYSLTVESTINVGSKFILRM"
     gene            complement(22353..23156)
                     /locus_tag="PLB33_01225"
     CDS             complement(22353..23156)
                     /locus_tag="PLB33_01225"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOG62172.1"
                     /translation="MKEKVINIAANDDVYTADIKKRLIKVLTENGYDYYEGFKEDAQL
                     NITVGGDGAFLRSVRESNFSQIPFIGVNTGTLGFYPEITPENLENFIINYRAGNYKIN
                     PVNLIECEIVSANLSNKIYAVNDIILKRIDMKTLHLNVFIASNHLEKISGDGLIISSP
                     LGSSAYNKSAGGSLVYPSLKTLQITPLSPILSNAYRCLECSIIVPPEFEIAIYPERRE
                     DYCLAILADGEIYEYDDMEHVLFRTSKKVINRLSTGTFNYWNVIKEKFL"
     gene            23285..24166
                     /locus_tag="PLB33_01230"
     CDS             23285..24166
                     /locus_tag="PLB33_01230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG62173.1"
                     /translation="MFNNILKYTIQENDETVKSILTEKLKFSGRLSRKLERNGKILLN
                     GKAVKLNKSIFAGDELSIEFDEEEDRYDAVDMPLDIIYEDDDLLVVNKEPHMVVHPTR
                     SHQNDTLANGVAYYFKKNNIKRKVRFINRLDMNTSGIVLIAKNPYIHNQMVLEMKSSM
                     VEKYYYAIVEGLLEEDEGRIDVPITRLNPEDIIRTVHTSGKECITEYKAEKRYNNMTL
                     VKLKLITGRTHQIRVHLKHIGHPIVGDTLYGNESLLIDRQALHCYEMKFKHPITNKEL
                     IITCPMPEDMKRVIEGR"
     gene            24175..25170
                     /locus_tag="PLB33_01235"
     CDS             24175..25170
                     /locus_tag="PLB33_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOG62174.1"
                     /translation="MKKILMIATGGTIASKITEHGLAPAISSDELLSYVPEIKKYCYV
                     DTIQLLNIDSTNIQPEHWVMMTETIEENYDKYDGFIVSHGTDTMAYTAAALSYLIQNP
                     CKPIVLTGSQKPINVDITDAKKNLLDSFRFVTEEGISGVYVVFDGKAIIGTRARKVKS
                     KSYSAFESINFPVAAIIDDRRITKYISNYNLEKKVKFYKEIYTNIFLLKLAPGMEPDV
                     LDFIGNKYEGIVIESYGAGGLPFLDKRNFLNKLEELIKKGKIIVVATQVMFEGSDMGL
                     YEVGVKALQKYNVLQAYDMTIEAVITKLMWIMAQTKDFEEVKTMFYTRIHEDSLY"
     gene            25216..26028
                     /locus_tag="PLB33_01240"
     CDS             25216..26028
                     /locus_tag="PLB33_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG62175.1"
                     /translation="MKKVDLHIHTNASDGTWDVTELKNELKKNKIDIFSVTDHDSVEN
                     IENMQNILKNKDNLIFIPGTELSAEYKGKEYHLNLYNYDIKNKELLELMKWTNQNKLN
                     SNVDYINYAANKYKNISVEDFERYANDRKRGGWKSANYMIDKGIHKDIPEHLKDVYES
                     GLKAELKSPEEVINIAKKSGGKIFLAHPSYHYRDSVMPIEELKHWGELGIDGIECYSP
                     YNRDNTRYYIDFCKKNNLMISGGSDCHGSFIPSRKLGVPDVSLDDLNIKKLL"
     gene            26057..26749
                     /gene="ispD"
                     /locus_tag="PLB33_01245"
     CDS             26057..26749
                     /gene="ispD"
                     /locus_tag="PLB33_01245"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOG62176.1"
                     /translation="MQNKKHISAIIVAAGKGKRMDSDIPKQYMDIAGKTILDTTLYKF
                     EKSNDVDDIILVVNKEDIEFVKDEIAPFYRKIAYVVPGGKTRTESVYQGIKKVSKNCN
                     IVLIHDGVRPFVSYNLISTCVENAIIHKACVPVIDVMDTIKEVSEDGMVVKTFDRKKL
                     RAVQTPQAFDYDIIRECYEKAMTEEKEFTDDASIVEYYGYKVKTIEGLYKNIKITTPL
                     DLRMAEIIVNFF"
     gene            26760..27239
                     /gene="ispF"
                     /locus_tag="PLB33_01250"
     CDS             26760..27239
                     /gene="ispF"
                     /locus_tag="PLB33_01250"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOG62177.1"
                     /translation="MKIGIGYDVHALAENRKLIIGGVKIPYEKGLDGHSDADVLIHAI
                     MDSLLGAMGKGDIGRLFPDDDAKYKDADSRVLLRKVYEIMRENKFTVGNIDAVIIAQN
                     PKLSPFIDEMRRNIASDLKTDISSINIKATTTETLGFIGRGEGIGAECICLLETKEK"
     gene            27457..28878
                     /locus_tag="PLB33_01255"
     CDS             27457..28878
                     /locus_tag="PLB33_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG62178.1"
                     /translation="METYQIISIIGVIIFIYFIFFKNRRSAQKEISSLNEIEYIGSST
                     MKFDDVAGNYEAKQSLLELVDFIQNPEKYSKFNVRMPKGVLLYGEPGTGKTLMAKALA
                     GEAGVPFYAVSGSDFVQVYAGLGAGRIRNLFKKAKKSGKSVIFIDEIDAIGKKRMSGS
                     IKGSDEGDRTLNALLTEMSGFADDSGTVVIAATNRIDTLDSALLRPGRFDRQIEIMLP
                     DKNARKDIIKLYLDKKNAEDINIDELADLTVYFSGAMIENLINEAALLAIRQNSDKIK
                     EEHIKKAYLQILAGVEKEDYDNGFQKTITSYHEAGHAFVSRHLLPENKIIRVSVIPTS
                     KGAGGYTLSTPDSEEVMTKGKMINHLSVLMAGRASEEVFFGNENITAGAQNDIEKVTS
                     LAVDYVSKYGMNDATFINYQLLSDKLRIPLTSVEVSVKKLTSEIYEEVIRLIECNKEK
                     VEKIARLLMEKEVIYQDELDNLI"
     gene            28988..29161
                     /gene="ssrS"
                     /locus_tag="PLB33_01260"
     ncRNA           28988..29161
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLB33_01260"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            29370..30371
                     /locus_tag="PLB33_01265"
     CDS             29370..30371
                     /locus_tag="PLB33_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG62179.1"
                     /translation="MITIKDVARLAEVSISTVSRVINDSKPVSPEVRKRVLKVIEETG
                     YKPNDVARSLVTRRSYLIGVIVNNLANSYVADTVRGIEEIGKMYGYDILLCSSYGSKE
                     TQEKYLQLLDRKQAEGLFLVGSIFDEEIIKLAKSFNKPCVFFTRDIRENMNYITIDCK
                     SAIYEVTKYLFEEGHRKIAYFSDYENRTTVEDEKVEGYLKAIEDNEMEYSKIYVVGGS
                     KHNEAYDLGKSIIDEAKDFTACVCSSDEIAIGILDSFLDNGIKVPDDVSIVGFGNIRE
                     GKFVRPELTTIGEPYYDVGAVGMRMLIKMIKGDKIQQGRMELPFTIEKRKSVKNLNK"
     gene            30489..30953
                     /locus_tag="PLB33_01270"
     CDS             30489..30953
                     /locus_tag="PLB33_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62180.1"
                     /translation="MKKRFIFTMIVLLLFSYNSINGYEYHKEIETIEYLLNERITIMN
                     EFLYGYKDMQSLEEKLSKIESDNLLQNDLDILYKVKNSPTDYELAMKVKVNEIYDIEL
                     TDDGYLVNADLNWLMRGYDGEFNLIRNYQIKCIEDGGQSYLAKLTVLNNTAE"
     gene            31134..31616
                     /locus_tag="PLB33_01275"
     CDS             31134..31616
                     /locus_tag="PLB33_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG62181.1"
                     /translation="MQNEFLQSKKSEFAELKEYIDSVKNSQGVTMQILQKAQSLFGYL
                     PLEVQQFISDETNIPLADIYGVVTFYTQFSTEEKGKHKIGLCLGTACYVKGSQGILDK
                     ISDELNVKVGKTTDDKLFTLEATRCLGCCGLAPVMMVDDDVYGKLDTTKIPGILKKYK
                     "
     gene            31628..31993
                     /locus_tag="PLB33_01280"
     CDS             31628..31993
                     /locus_tag="PLB33_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG62182.1"
                     /translation="MKSLEELRKIREEAIKNIEMRNPDKDIRVVVGMATCGISAGARP
                     VLSTFVDEVAKRNLKNVQVVQTGCIGMCTYEPIVEIYSPDKEKVTYIHVDSEKAKRIV
                     AEHLVNGRVVREYTIGSEI"
     gene            32018..33820
                     /gene="nuoF"
                     /locus_tag="PLB33_01285"
     CDS             32018..33820
                     /gene="nuoF"
                     /locus_tag="PLB33_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOG62183.1"
                     /translation="MAIFRAHVLICGGSGCTSNKSQVVRKRFEEKIKEVQLDKEVQVV
                     GTGCFGLCEEGPVVIVYPEGSFYSRMTVDKVDEIVEEHLLKGRIVEKYLYKEKVKVED
                     AKEHKSLMEVQFYSKQKRVALRNCGLINPEDINEYIARDGYMALGKVLTEMSPQDVIQ
                     LMKESNLRGRGGAGFPVGLKWGFAAPNKADQKYIICNADEGDPGAFMDRSILEGDPHA
                     VLEAMAIAGYAIGATKGFIYVRAEYPIAVRRLHIAIDQARELGLLGKNIFDSGFDFDI
                     ETRLGAGAFVCGEETALMQSIQGNRGMAKVKPPFPANKGLWGKPTVINNVETLANIPQ
                     IILKGPEWFKSFGTEKSPGTKVFALGGKITNTGLVEVPMGITLREVIYDIGGGCPGGK
                     AFKAVQTGGPSGGCLTAGHLDTPVDYENLAAVGSIMGSGGMIVMDEDNCMVDIARFFL
                     DFIVDESCGKCTPCRVGTKRMLTILERITQGKGTLEDLEELESLANSIKNSSLCGLGQ
                     TAPNPVLATLRYFRDEYIAHVVDKKCPAKNCKALLTFEIDKDKCTGCTLCARNCPVSC
                     ISGTVKNPHEINQELCIKCGNCYSVCRFGAVQKN"
     gene            33836..37288
                     /locus_tag="PLB33_01290"
     CDS             33836..37288
                     /locus_tag="PLB33_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG62184.1"
                     /translation="MALVKLNINGKELTAEAGKTVLEAALENGIEIPHLCFDERLEVY
                     AGCGLCIVEIEGQPKIARACATPVSDGLVVRSDTQKVIEARNNALNLLVSQHIGDCKA
                     PCTLNCPANTDVQGYVGLVANKQYIESLKLIKEKLPLPACIGRVCPHPCEKACRRQYV
                     EEPVSIACIKTFAADYDLNTGNPYIPELKPATGKKVAVVGAGPAGLSAAYYLLADGHE
                     VDIFEAMPKPGGMLRYGIPEYRLPKNVVDAEVAIIEKMGANFNYGVKVGEDISLEYLQ
                     NKYDAVFLGIGAWKSSPMRCEGENTPGVLGGIDFLIKVAENKPVKIGEKVIVVGGGNT
                     AMDVARTSIRLGAKEVRVVYRRSEEQMPAEEIEIKEAKEEGVIFSFLSAPSLVLSDGE
                     KVTGLKCEKMVLGEKDASGRQSPLPTGEFVEFEADTIIAAIGQEVDCGKINVGQGKKK
                     NIVINEGTFETNLKGVFAGGDAATGPKIAIDAVAQGGRAAAVISSYLAGEMIPYKNQP
                     LVYTEVTKEDYKDEERIPRVPHRTVEPEIRKHNFQPILPTMTEKEAIREGQRCLECGC
                     KDYFECQLVDYIKKFEVDTKKYHAENEKKFKETDHPFISQNVDKCVLCGLCVRACDEV
                     VGASALGFVERGNATFIAPAFEQELKVTSCISCGQCIDVCPVGAWLDRRGNMKEIPLD
                     LTQTKSVCGYCSVGCDVVYEHKDNMVYLASSPKENEIPVCGKGKFGIEHINDENRILQ
                     SKVKVKGKYEPIELPVALFETAKRLRSIQNMYGDDQVAFVVSPKLTNEEFKYIKAVAD
                     ELNTKYKGSFTLDSESGIEYVLGKNESTIRAEELDNADLIISAGQLYEHHPAAGMKLR
                     RLSNTKKIISASTIKTKLNNFAVTADVTDYEVFFTRVLKALVDNGVIKKEAISRYENG
                     EELIKALDKTEVLPEAAKVAEAFKKARKPIIVADENTVPKAALKVLADITVLAGNNNR
                     PHRGLITLKAKANSQGAWNIGFRTPGEEIRKALLNNELKGLVVIGEDILGNVPELKKA
                     ADNLKFFAAFDIMENETTSNAEYVLPLCSIAESNGTIVSADGTVRNVYQAIKPLTGTS
                     NLEMFAKLAGLLNAEYKEDAKDEAKVKLYVPTLKGKEKEYEVYDTVEKAFLAKLKENC
                     IKAV"
     gene            37630..38418
                     /locus_tag="PLB33_01295"
     CDS             37630..38418
                     /locus_tag="PLB33_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG62185.1"
                     /translation="MKKIFKSILLTASLTIGLSMFVAADTYAATYTIAKGDSLYKISK
                     AYNTTVSNIMVANDLKSYDLSIGQVINIPDVKYIVKPGDTLYKISQKYNIPLFNLRQA
                     NNIWTDYIYVGQVLEIPLPPAAVKEELKVAKQPNEGYKDYTAEELDLLARLIMAETES
                     QSYEAKVAVGSVVMNRVKSGLFAPTISGVINQNINGYYQFTPVENGWIKRAANEECIK
                     AAKEALSGVDTTNGALFYYDDSTTNQWILSKPVSVKYGRMIFAY"
     gene            complement(38458..39780)
                     /gene="mgtE"
                     /locus_tag="PLB33_01300"
     CDS             complement(38458..39780)
                     /gene="mgtE"
                     /locus_tag="PLB33_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOG62186.1"
                     /translation="MLELIEQKKYIQLKKELSEMNEVDISEILDPLDLQTTLLVFRML
                     PKDTAAEVFSYISPEQKADIINAVTDKELKYILDELFFDDMIDLIEEMPANVVNKILI
                     NSTQEERTLINQFLKYPAHSAGSIMTIEYVELKKNMTVKEALEHIKKTGINKETIYTC
                     YVTDKKRKLEGIVSLRNLVVSDPDQSINDIMQEEVIYVETHDDQEIIANIFKKYGFMA
                     LPVVDKEHRLIGIITFDDIMDVMEDEATEDFQRMAAMVPSDEAYLDLSVIALAKQRIP
                     WLMILMLGATVTGGIITKFENILSTVVVLTAFIPMLMDTGGNSGNQSSTLIIRGLALG
                     NVNQKDIWKVLWKEFRISLLVGIILASVNFAKNMLFDKVGLNVSLTVSLTVATTVMLA
                     KMVGGTLPLIAKKLKLDPAIMAGPLITTIVDALVLLVYFGVASALLIK"
BASE COUNT        14587 a         5765 c         7861 g        11471 t
ORIGIN      
        1 gcataacata tattcatact gcttatgatt aaaagagcga taaatataat tgtaatcttt
       61 ttcataattt ctcccacttg tctttatgca tttcaagctg ccttatatac aaataacgag
      121 taataatcat ttcgatatta ggaggcatct cttcaaaaat acatccgtaa ttgtagaaag
      181 ataaatactt ttgattatcg tcatatattt tatcaattct cataacctta gccttcaaca
      241 atacagctgt tttttcacat ctaaaataaa aagctatgat atcatttatg tataattcgt
      301 agtttgatga aatcaacatg ccgccaatgc ttaagttaat caaattaatt ttcatatttg
      361 gaaaatcttt tgttatgtct ttatcatttc tgtaaagact ttctatataa taggataagt
      421 cggtccgaac ctttaatgag tttcttcttt caatatcggg cttcttttta actatatgag
      481 cattaagctg attatcggat gccaaatcaa tctggcagca gtaacgggaa attcctacaa
      541 attcactgta aatttcaata ataacatttg ttttccggct taaaatcggc aagccggtac
      601 ctttaacctt tattattttt tcatttaagc ttaacagttg accttttcca atcagtttac
      661 tatcttcatc atatattttt actattttat cagtatcaat catgattact cccaagctgt
      721 tatttttaag cctcactctc aggcatttta aaattcaaga aataaacata aatatctaca
      781 attgagctga ataattcttc gggaatttcg ctgcctattt taagctggga caatatggct
      841 gacaaggagt catctctata gacaggcact ccgttttttt cggctatttc aacaactttg
      901 tctgcaatat aacctgaacc tgaagctatt acgacaggag cgttgttttt agccgcatta
      961 tatttaaggg caacagcatg atttgattta ttattatatt gtgacatcta caccattttt
     1021 cctttcagtc agatttgtga aaacttcgct aaagcgcctg gctttaatgc attcctgcac
     1081 tcttatgctt tctattgtaa cattgttttt gcttaagaga aagtttaaat cacttttaat
     1141 tttctcggtc tgttccgata aactgcccgg tatataaata tcaagcgaca agacggagtc
     1201 ccttaatttt aaagttgttt caaaatatcc taagttttgg atatcaaaag ttataaaaac
     1261 cttataggat ttgtgatttt ccaggtttct tttattatca gggctgtctt catccttgcc
     1321 tatccatatt tcggaaaaca taaacatacc cttgtaattt aacggtaaaa acatgtggag
     1381 taagggaata ttaacatttt gactaaataa aatcgactcg gtcatatccc ccattaaacc
     1441 tttagttaca gcattctcac tttcgcttat ccctttatca aggagtttga aaaaggattc
     1501 aatagaatcg tttttaatat ttgattttgt ttcataagta ttgattaaag ttgtttttaa
     1561 taattccatc tgttttccgt caagttgaaa attatatttt aagaattcaa acaaattttc
     1621 caattcatca gaaaaattag tttttgagcc atattccaat cttaccaggt tgtgaaccaa
     1681 tacggataca tagtccctta taataccaaa atcattcatt ttggaaatat atcttccaat
     1741 gaagggcaga atttcatttt tcaacagctc caagtttgag tccatggcat ttgtattatc
     1801 gtaaataaat ttttccgtta gctcattaaa agaagctttt aacatttccg gaatgttttt
     1861 ttcgatattt ttaaaggttg tgttaattga gccgtatatt tctttcactg atacaaagca
     1921 gtcatagctt cttaagaaat tttttaaaac cgtttgaagt cctttgttgt caggattatt
     1981 gcctaacaaa gttctcagta aatcgaaaaa ttcgccttga aatttagtgt atgttccttg
     2041 ctggaactta agaaagtcca atatttcagc ttcattcatt ttaatattac caagaaaaga
     2101 atcaaaaaat acacttaaca ccgggtcgtt tttaatattg ttgttgataa actgtttgct
     2161 taaaaggagc ttcttaactt cttcatataa tacaggagaa ttttgcaatg atttaataaa
     2221 cttgtcaaaa acagaatcag gattgtaatt gaaaagttct tgtctgattt gattttcata
     2281 acccttctca ggcgtctttg taaccttagt aggatcagct atatcaaaag gcacttcgtt
     2341 tttaatttta ggcttattaa tgggattttc gctaatatga ttattagtct tgacacttat
     2401 attattcaaa ataatctcct gttttttaaa gttaatacca attgggggac attccttatg
     2461 ctccaaagac taaccatatg gtaaaggtgt gtcccccctc atcttgtata tcggcacgat
     2521 aataaattaa ataagagtat tatcgaaaaa tttataaaaa gtttttcgtt ataatagctt
     2581 aacaattcat aataaaggtc gatataaaga taggaaacca acaaggagag taatatggat
     2641 aataatttag attctgttct tgatatgtat ctttttgaaa caaattcatt attggaacta
     2701 ttagataata ttttgttaaa agctgaaagc aatttaagtt ttgataaaga atgcatggac
     2761 gaaatattca gggcaatgca taccataaaa ggctcatccg ccatgatgca atttgacagt
     2821 ataatgacta tttctcacaa aattgaagat ttgttttatt atatcagaga aaatggaata
     2881 gaccaaaagt ataacgaaga acttgtaaat ttagttttta aaattactga ttttataaaa
     2941 ggagaaattg tcaaggtaga agaagggctt gagcttgaga aaaatttaga ccctttagaa
     3001 aatgaaataa agttattttt aaactcaatc aaaaaagaaa ccaaagaaga aatataccaa
     3061 atagaaaatc taaaatcaaa tattgcaatt actgaagaaa ataaccaaac aaagggatgc
     3121 acatacgcca taagaataat atttgatgac gatgtaagca tggaaaattt aagagcattt
     3181 attatagtca atcaattgag ggaaacaggt attgaatttg aatataaacc tgaaaatatc
     3241 gaaaacattc ccgaaacatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 gaaagcttta aaaaatcaac aagacaagtg agaaaactta ccgatgagct gcaggatatt
     3421 gtaatgtcct tgaggatggt tccaatttcg ggcacattta ataaaatgca cagggtagta
     3481 agagatatgg gaaaggaatt aaacaaggat gttgagcttg tattaaacgg tgaggatact
     3541 gaaatcgaca aaacaattgc agattgcatt tcagacccca ttatgcacct tgtcaggaat
     3601 gcaatggacc acggtataga atcggaagaa aaaagaataa gcgcaggaaa gaacaaaaaa
     3661 ggaaaaataa ccttatctgc ctttaataca ggaggagaaa tacacattac cgtagaagat
     3721 gacggaagag gatttgattc ggaaaaaata ttggaaaagg cggaagccaa aggtttatta
     3781 accaaaccag caagcgaata tacacaaaaa gaagcattcc aatttttgat gcttcccgga
     3841 ttttccacca atgatgatat aaccgagtat tcaggcaggg gtgtcggaat ggatgttgtt
     3901 aaatcaaatg tggaaaaggt gggagggaca attgtattag agagcaatga aggattaggg
     3961 agtaaaatta catttaaaat tccacttact ttaaccatag tatcaggcat gcaggttgta
     4021 gtgggaaact ctgaatttat aataccgatt aagagtattc atcaatcatt taaaaccaaa
     4081 aaaaatgaaa ttattcataa tcccgagcag ggcgaaatga tattgataag ggaaaaatgc
     4141 tacaggataa ttagaataca tgaatatttc aacattaata cagatattac aaatatcgaa
     4201 gacggtatat taatatttgt tgaatcaggt gacaacagtg cctgcttgtt tgttgataaa
     4261 ataatagggg agcagcaagt tgtcgttaaa ccccttccaa taatattaaa caagtacaac
     4321 cttaaggaat ctggaattta cggctgctca atattaggtg acggaagtat caatttaata
     4381 ttagatgtag gaaatttaat ggaaagtaat agatatggtg agaataactg ataacgaatt
     4441 tttggaatat gtaaaataca taaataagaa ctacgggata gacctttcaa agaaaaagat
     4501 attaattgag gggagactat ccaatttaat tgaaaaaaag ggaatgaaca gtttttacca
     4561 gtatttgaga agtgtaaaaa acaacgatgt agaagaaaca atgtttgtaa acaagctgac
     4621 aacaaactac acatattttt acagggaaga aaaacacttt caatatttaa cggaaacaat
     4681 acttccatat gaagaaaaaa ataataaggc taagatattg aatatatgga gtgcaggctg
     4741 ttcatcgggt gaagaaccct atactatagc aatgatattg gatgattatt tcaagtttcg
     4801 gattaatcaa tggaaaatac gcatatatgc tactgatata tccgaaaatg ttctttcgaa
     4861 ggcacgtgaa ggaatatatt ccaaagacag catctgtaaa cttcccgatt catttataaa
     4921 aagatatttc ataaagctcg acgataataa ataccaggtg gtacccgaaa tcaagaatta
     4981 tgttagattt aaagcatcta atttaatggc tccgatattg gaaaaaaaca ggtatcacgt
     5041 aatatttatg agaaatgtaa tgatttattt cgatttagaa acaaaaaaca aaattataaa
     5101 caaattatac aatgcaacca ggtcaggggg atatttgatg attggacatg cggaaataat
     5161 tcaaagagag cagtcaaaat atctctatat taaaccggcc atatacaaaa aggaataata
     5221 tgttaataaa taaaataatc gtaataggag cttcaacagg aggtaccgag gcaattatca
     5281 aaatacttaa agaattgccg gaggattttc ctccgattct tatagctcag cacatgccta
     5341 tcggatttac aaaaatgtat gcggacaggc ttaataagat atgcaaaatg gaagtaagag
     5401 aagcccttga tggtgacagg ataaaacaag gtgttgtatt aattgctccc ggggacaagc
     5461 atatggtgct taaaaaagac gataaaggtt actatgtatc ctgcaaaaaa ggcgacaaaa
     5521 taaacggata ttgcccttca atagatttgc tttttaattc ggcggccaaa attgccggta
     5581 ataaatcagt aggaattatt cttaccggaa tgggcaaaga cggagctgaa ggtcttttaa
     5641 atatgaaaaa agcgggagcg tatactatcg gacaagatga aagtacttct gccgtttacg
     5701 gaatgccgat ggctgcattc aatattggag ctgttacggt gcaggccaag ttagaagata
     5761 tttttgaaat tttaacaaaa catttaacaa tataaaattt ctgacgataa tataagtagc
     5821 acgaagataa agcaaagatt attgagtaaa aaggagaaat tatgttaaat tcattggagc
     5881 aatacaaaaa aacctcagta aatacaatga caaaaggtga gcttctgatt cttttattcg
     5941 atgaagcaat taaaaaacta aatcaaagca aattattaat ggagcatgaa gactatgcta
     6001 atgcaaacat tttcttaaca aaagtcagac atatatttta ttatttaacg gctacattgg
     6061 atggcaagta tcagatatca caggagttat ccgatatata cgaatttttt aacagagaaa
     6121 ttatgaaagc atcaacatta aaatctacaa aacatataga tgacatattg ccgattatta
     6181 aggatctaag agacacttgg gctgaagcag atatactttc gagaaaaaag tattagaggt
     6241 atgctatgga agagttatta agaaacaaac tggaggcagc caaagaatta aaaaagttaa
     6301 catcgtttgt caatgaacta tccttaataa tcgattataa ccgggttaat tcattgcttg
     6361 atgaaaggca gcagtatata gataaaataa atgtaattaa cgaaaaaata agcgaagtaa
     6421 aaagcaagga aaactatgtt gaaacaaatg aaattaaaaa attaaacaaa gatatcggca
     6481 gggtttttac ggaaatttat gaaatcgata aagtaattcg taaaaatata aataccgagc
     6541 taaaatcagt taaggaaaag ttaaattact cagaaacaaa catagttgta aatattaaga
     6601 tataaacatt ggacaaccat atcaatatca tgtggaggga ttatgaaaac gaataagagt
     6661 atttccaaaa agataacaac tactatttta attgtatgtt tttcggcact gctaataaca
     6721 ggaataatta ttgttaaaac agtcagtaca aggtttaagg ataacaaaag acaaatactg
     6781 aatgaaacgg cgttaagcat aagcaataag gcagaagttt tttttgaaaa atatattacc
     6841 gttgtagagc aaatggctca tgacaagaac ttacaagaat ttatgattaa tgcagaagga
     6901 taccaaacat tacctgtaac aagagggttc gacatagcta cggcaacaac tgatgatatt
     6961 caaaaagcag attcagaaat aattcttact gcttatattg cagaagatga ttattatttg
     7021 gcatctccaa ccatctatac acaatccgaa tatgatgtta cttccaaaga atattacaat
     7081 gcagttacag aaaataaagt atgcattacc gacccttata ttgatgctat tacaggagga
     7141 atagttataa ccatatctgc accggttaat gtagacggtg aaattgtagg cttgacggca
     7201 gtggatatag ccattgataa attaataaac atggttgatg aatatgagct gggagacaca
     7261 ggatatttca cattgttatc aaaaaataat gttataattg cccataaaga taaggataac
     7321 ttattaaaaa aagttgatga aataaactta agcgataata tcgttcaatc agttatcaat
     7381 aaagacaggt ctgtagtaga atttacatac aataatgacg ggttcatcgg aaacagtgtg
     7441 gaaatcgggg atacggggtg gcagataatg tcgtcgcttc caaaagaaga attcatgttg
     7501 aatacaaatc agttggtatt aattattata gctgtattcg taatattatt ggttgtgctg
     7561 attggtgttt tatacgtatt aataaataaa ttgacaaaac cgataacgga tataacaaac
     7621 attaccaaca aattggctga aggcgaatta gatgtaaata ttgatataaa cagcgacgat
     7681 gaaatcggtg tacttgcaaa ttctataaga aaactgacag agcggttaaa attatatatt
     7741 atttatattg atgaaagcgt cggagttatt gataaaatgg ctgaaggaca tttgaacatg
     7801 gagcttagaa atgattatgc aggtgaattc gccaaattaa aaaaatcctt gttaaatatg
     7861 tccaatacct taaaagatgc aatagggaaa atacaacaat cctcaattgc aatcaacatg
     7921 aatgccgagc aggtttcaac gggagcgcaa acattggcgc aaggaacaac ggagcaggca
     7981 agtgcaatag aggaattatc agcagaaatc aacgaaattt acacaactat cgtaaacaat
     8041 gcagagtatg cagaaaatgc agggagcaag gcaatgcaag cttccaagga agttgagcgc
     8101 ggcaacatgc aaatgcgcga aatgctttca gcaatggatg aaataagcaa ttcatcaagc
     8161 gaaataggaa agataataaa agttatagat gatatagcat tccaaactaa catacttgca
     8221 ttaaatgcag ctgtagaagc tgcaagagct ggtgcagcag gcaagggatt tgcggttgta
     8281 gcggacgaag taagaaattt ggccggtaaa tcggcggaag ccgccaaaca gacaaccgca
     8341 ctgattgaga attcaataaa tgcaatcaac aaaggaacaa tgctggcagg cgaagcaggc
     8401 aaatcattgg caggtattgt cgataaaaca aacgaaacaa atgagctgat aaatgaaatt
     8461 gtaaaagcat catcacagca gactatttca gtaaatcaaa taagaagagg cattgagcaa
     8521 atatcatcgg tagttcagga gaatgcggca acagcagagg caagtgcagc caacagcgaa
     8581 gaattatcag gacaagtatt gattctcaac gatttagtaa aacaattcac tctataagct
     8641 tactttctgt acaatataaa atacaaccat aaagctatgg ttgtatttta atttatttca
     8701 taatttgttt aatttattgg aattattgac gatatatgaa tagatagata tgaagacaag
     8761 gaaattataa tatagaagaa aaggagaaag atatgtcaat actttcaact aatttatacg
     8821 gctcatctgt aaacaaagga atgggcggcc ttatgtcagg attggatacc gatgacttgg
     8881 tcaatcaaat gacagccgcc accaggaata aaataaataa gcaatatcaa gcaaaacaaa
     8941 aattgttgta caaacaggaa gcatacagag aaataagttc aaagcttcta gcctttaaca
     9001 ataaatattt ctcttattca tccggctcaa agactaatat tctaagtcct aagttttttg
     9061 aatcatatac atataaatcc tcatctaatt acgtaaacgt gaccggcagt gcagatgtta
     9121 taagcaattt taaaatcaat agtataacat cggttgctaa agctgcaata tatacaacaa
     9181 attctgtttc atctcaaagc ttttcaagtg atgaaataat cggtaacata tcggaagatg
     9241 ttttattaag cagcagcatt acatttgatt ttaacggagt gaagaagact atagctcttt
     9301 ctgaaatggt tgacggtaag cctgacggtt caccttatac ttatgatgct gaaggattag
     9361 cagattttct gaatacaaaa cttgctgaag tttatggagc cggaagggta aatgcagaac
     9421 ttgatgcttc ccaaacttca ataatgtttt ctgcttccgg aagtaccgat gtttttagtg
     9481 taagcaatat cagcaatgat ttaagcatac taacaggaat taaatcaggg gacagcaaca
     9541 gattaaaaat ttcgaaaact ataggtgata tatatggaga agtaacagat tgttctatta
     9601 ttattaacgg aaaatcaata gatggaatta gtaaggatat gtcaatttcc gaagtgctta
     9661 aaaagataaa caaagaatct gatcttgata ttacatattc caataccacg gataaatttg
     9721 ttatcgtgtc taaagatacc ggtaaagcat caaaaatcga aattacggga gcattggcag
     9781 ataagctttt cggaggaagt attggcagtg acatagaaat aagaggcgaa gacacagtga
     9841 tgaatgtaac cattcacggg caagatatag atattacacg aagcactgct aatttctcaa
     9901 ttgatggtat aaatattgaa ttgagcaaaa atgcaatact tgcacctgaa gaagacccta
     9961 taacttttga tgtaataaat aacactgatg aagtgattga aagagtgaag cagtttattg
    10021 atgattataa tgaaataata gatttgctcg gcactaaaac aaaagaaaaa ccaagaagag
    10081 attatcctcc attaactccc gagcagcagg atgaaatgaa agaaaaagaa atagaaaatt
    10141 ggttaaagga agctaaaaag ggagtactct tcggcgataa gaatattaca agcctattga
    10201 acaaaatgag agcaagcatg agcggtatga cctccgtaag cgacttggct ttgtccaata
    10261 tcggaatttc agcagcatca atggatacca gcggtaagtt ggtttttaat gaagagaagt
    10321 tcagagaaaa gttaatagaa gctcccgatc aaatagcaag cttatttaca ggtatagctg
    10381 atgaaacgga tgaatatgca aagtccggca ttgcaaatca aattcttgat atacttagag
    10441 aaaatattgg agctatgggt acatccggaa agttaatcga agaagcaggc ttagacaccg
    10501 gtcgttcatc cgaccaaaat aacatttcat taaaaattaa agattatgat gaaaaaatga
    10561 aagagttaaa gaagagtctc gaaagagaaa gacaaaggta ttggaatcaa ttttcagcac
    10621 tggagcaaag tctgaacaaa ttaaatgcac aaagctcatg gcttatggac atgatgggtg
    10681 gttattagac ttaaaaggag aaaataatga agattgtaat attagcaggt ggatttggta
    10741 caagaataag tgaagaaagc catttaaaac ctaagccaat gatagaaatt ggagatgctc
    10801 cgatactatg gcatattatg aaatactatt catgctttgg atttaatgac tttattattt
    10861 gttgcgggta caaaggttat gttataaaag aatattttgc cgattactat ttgcataggt
    10921 ctgatattac ttttgacttt tctaatggta atgaaatgat tatacataat aatgttgctg
    10981 aaccttggcg ggttacggta gttgatacag gactctatac ccaaacagga ggtcgaataa
    11041 aacgcataaa aaaatatatc ggtaatgaaa cttttatgat gacatatggc gatggagtta
    11101 gcaatataga catacataaa ctagtggaac aacataaatc gactaataat attgttactt
    11161 tatccgccat acagcccggt ggcagatttg gtgttttgga tatagatgaa aatgacagga
    11221 aggttatagg atttagagaa aaggcgactg aagacgcagg atggataaat gcaggattta
    11281 tggttatgga gccagctgta tttaattata taaacgaaga taaaacaatt tttgaaagag
    11341 agccccttga aaatttaagt gccgagggga agcttggagt gtacaagcat tatggattct
    11401 ggcagtgcat ggatacacag agggataagt atcaccttga atcgatgtgg ggaaaaaata
    11461 tggcaccttg gaaggtatgg tagatatgaa aattagtgtt attatgctta cctacaatcg
    11521 tgaagagctt ttaacaagag caattgacag tatattaaat cagactttta aagattttga
    11581 gtttataatt gttgataacg gctccactga cagaagtgga gcaattgctg acgaatatgc
    11641 attaaaagat tcacgaatta agataatcca cagagaacaa ggaaatattg gaagtgggcg
    11701 aaatgcaggt ttggatgctg ccacaggaga gtatgtagcc tttatagatg atgatgacta
    11761 tgctgaatct gactttttag aatttttata taatcttgca aaaaaacata atgcagatat
    11821 tgctgtatgt ggttcaacaa aagaggaaaa tggtcaacaa ttaccaaatg gcatttatca
    11881 atatgatgag aaatatatca tggatgcgga acaagcaacg gtaaactttt tatggcgtaa
    11941 gctttataat gcagccatgc cgactaagct tgttcgacga gagatgtttg atagtattcg
    12001 ttttttgaac gaaggcaaat atgacgatat aaccacaacc tatcgttatt ttgctaatgc
    12061 aaaaattgta gcagcacatg gacttcccag atattgtttt tatcgccact ccggtaacaa
    12121 ctcaagtgca gcgacaaagc accatttgct caatccggtt caattgaatg aatacttagc
    12181 tgcgtttcgc gaacgaaccg aatatataag caaaatattg ccccagttag cgggtttggc
    12241 tctttacagc gaatggtctt atatgatttc aatggtagag aagattcata gatatggtct
    12301 caacaattgt gctgatttac tagtgcttat gtgtgataat ttacgagcac attgggatga
    12361 tttttattat ggcaaatata ttttggagtt cgaaaaagtg tggatggaga ggtatgtcaa
    12421 ataatcaaag acggagtaat tctaggatga ataatattaa tttttctagg gcattggaaa
    12481 agagttgctg tacgggttgt gccgcatgtg caaatgtatg tccaaacaat tgcattttca
    12541 tggaatatga tgatgaaggc tttttatatc ctgaaattga tataaagaaa tgtacggagt
    12601 gcggtttgtg cgaaaaatca tgtccggcaa tcaataatcc tgtaaatgat aattttatcg
    12661 agccggaagt atatgctgca tggagtcttg atgatgaagt aagggcttcc agttcatccg
    12721 gaggaatatt tacggaatta gctcacaagg tgcttgataa tggaggtttt gtttcagggg
    12781 ccagatataa aacaaataac ttagttgaac attgtctaat aaatagcaga gaagagctac
    12841 atgagctaag acagtcaaaa tatgttcaaa gcgaaatagg attaattttt agggacataa
    12901 aaaagctgct agaagaagat gggactgtaa tgtttacggg aacaccttgt caatccgcag
    12961 ggttgctgca attcctcaat aaaaaaaatg ataatttgct tttatgcgac attgtatgcc
    13021 atggtgttaa ttcaccaatg gtttatttaa agtattttaa tgaattagaa gaagaatata
    13081 aaagcaaaat taagaaaatt aattttcgtg ataaaagaaa tggttggaaa gattttggta
    13141 catctgttgt gtttgaaaat ggaaaggaat atttttgcag tcagaaaaga gaaccttttt
    13201 atagaggatt tattaaaaac ttatatttgc gtccatcttg ttataattgt caatataagt
    13261 catttccaag gctttcagat ataacattag ctgatttttg ggggattaat tttattgctg
    13321 aaaacgaaat ttataaaggg gtttctcttt tactcattaa ttccaataaa ggcaagcatt
    13381 acttagaaga aataagtaaa tatataagca aagaacaaaa gacacttaag gatgctacat
    13441 taaaaaaccc atgtatactc aattctgtta ctttgaataa taaacgtgaa ttgttattta
    13501 aggctttgag gaaagaaact gatgttgtag atattataca gaaaattatt gattacgatt
    13561 aggagtaaat tattgtgaat aaaagagttg aattatatca tagtggcctt accattactc
    13621 agaaatgcac tttaaagtgt aaattatgtg ctggatattc accttattat aatccacaac
    13681 cccattacag tatcaacagg ctgtctgaga ttataaaaag gtatttttct atagttgatc
    13741 atgtagaaaa gtttactatt acaggtggag aacctttttt gcatgaagat ttggataaaa
    13801 ttttgattga gacaataaaa tatagaaaac aatttgggaa aatcgaaatt ataacgaatg
    13861 gaaccttatt tccaaatgaa aagctaaagg ctgtgttgga gaaatatgca gttgaggatg
    13921 gttgcattac attgttggta gaccactatg gtgagctatc aaaaaaaata catgagcttg
    13981 gactatattt tattgagaag ggtattaatc acaagatacg gatttattac ggagaaaatg
    14041 ctcattgtgg aggatgggtt gattttagtg atttttcaaa aaaattattt tcagtatctc
    14101 agatgatgga aaaatttaaa aaatgcgcta ttccaagtaa gttgaattat tgtttggcaa
    14161 ttaaagacgg agaaatgcat ccatgtactt tctcacacag aataatggag ttgaatttaa
    14221 ttgaacgaaa taaggatgag tatattgatt tatttgatga taacacgatt gaacaacaaa
    14281 aggaaaaaat aatgaagctt tttgacataa aatatctaag tgcttgtgct tattgtaatg
    14341 gaatgtgtga tgattctgta agatttgttc cggctgaaca attatagggg gaatatatgg
    14401 tatatatttt tacaacggca tataatgcgg aaaagacttt gcataaagca gttaacagca
    14461 tacttaatca aacatatacc gatttttgtt attatctttt tgataatggt tctcttgatg
    14521 atacaaggaa aataatcaag gaatatgcta ataaagataa taggattata ccactgtttg
    14581 gggaatctaa ttgtcgcaga gtaattccta aaataatcaa ggatataatt tcaggagata
    14641 atatgctaaa gaactcgtat tttgcaaccc ttgacgctga tgatgaatac tcaccggatt
    14701 ttctagaaaa gatgatagat tttgtggagg ataataatct tgaagttgct acttgcggaa
    14761 cagaatggat agatgaaaaa acaggacaaa caatcaagaa aaaagtactt aataagaact
    14821 taattcttga aggtaaagaa tttgcagata aatttccgct ttaccgtaac tttatagtca
    14881 cattatgggg gggtatttat tctcttgaac ttctcaagaa atgtgatttt gaatggtatc
    14941 acaatgccat gaatttttct gataccgcat tttgcatgga gacattccga cgagctaaac
    15001 gggcgggtgt tcttagcgag agtctgcata aatactatat ttcaccaagg acagtgtcat
    15061 ataaatataa tccggactgg ttttatgctt gtaaatattt acataaaatc agtagagagt
    15121 atcttcttga ctacggtgaa ataagtaagc aaaatgagaa ttatttgtgt gttatatttt
    15181 taattttgat aaaatatatt ctccctaaaa tacagcaggc agatgttaaa ttaccggaaa
    15241 aactcaaaag tttgcacaat atttttacag acgatatgac gaagtatgtt ttagagcatt
    15301 gggatgaagt cggtatatat tctaataagt tagatttcct gcgtgaaata gaatcatggc
    15361 tctatacaca agatggctgg ggattattac gaaaaactgt agaggaaatt ataacagcca
    15421 tgaatatctg aattaaagaa agacataaaa tgaaaggtag ttgtaagatg agtaaaatta
    15481 ttatatatac accggcatat aatgcggaaa aaacgctccg tcgtgcagtt gacagtattc
    15541 tgaatcaaac acataaagaa tttatatact accttcttga caatgcttca acagatggca
    15601 catatgagat tataaaagaa tatgctaaaa atgataagcg aattattcca ttacataatg
    15661 aaataaattg ggtcggaaat ggtatttatg aagttttaaa aaattatggc aaagattgtt
    15721 atttctgtgc acttgatgct gatgatgaat acacaccaaa ttttcttgaa aaaatgcttt
    15781 tttttattaa aaatgaaaac ttagatattg cagcttgtgg cagtgatttt gttgatatgc
    15841 aaacaaataa acatcttggg atgagattgc ttaaaaaaaa tcttatttta gacgaggaaa
    15901 aatttgatag atattttcca acatatcatc aatttatgcg gacaatttgg ggaaaagtgt
    15961 attctatatc agtattacgt aggcctgagt ataaaagata taaacaaccg ggttatggca
    16021 atgatacact ttttgcaatg aatgcatttt ataacgccaa ccgtgttgga attttatctg
    16081 aatcattaca taaatattat gtttctccaa aatctgtttc ttatcaattt gataaaaatc
    16141 gtacaaaatc tgatcgtgtt ctttttgatg ctgcttgtga ttttttgatt tcaaaatgtg
    16201 gagcagtcag tgatgaaaat cttaattatc tatggattgt atatttcaaa gcaatcaagg
    16261 atacgttaaa tgttttacta aatgctcaaa taagcgtatc tgataaattt gaatatttgc
    16321 tcgatatatt tcaaagccgt catactcaag agcttatcaa atggccggga gcaaaagcgg
    16381 agaaaaaccg gctatttagt caggcagcat caaggatgct aagcagttca ccttatcttg
    16441 tcggattaga ggtagaattt ttatgttatt ttaaggatat tgttttttct attctgcaaa
    16501 atgatgaaga aaatgcactt agtcaaatta tagaattaat tgcagaagga acagaaatcc
    16561 ctgatgaata tgtagagtct ttgatgttgc tggccttaaa cttgtctgct aaattagaat
    16621 tacatgatta ttttatatat tttaagaaac tacaaatatc cttgcttatt gaactttcta
    16681 aaactaatga agcaataaaa gagtttgctg attgggatga gattttatct gacgacatag
    16741 attttaaaaa tcttaaagag aggttgggta tatgaaaaag tatttattat atgggcatgg
    16801 cggtgcatat aatcatgggg cggaagcaat tgttaaatct actgtagacg tgattaaaaa
    16861 gagacatccg gacagcaagg ttatcctatc aactcatttt aaagaacaag atattgaatt
    16921 taatatgcca atcgatgaat attgtgaacg tgatatgaag tatgttgaat tggacaaaaa
    16981 ttcctcagaa aaaggaattt atgatagatt tatctataaa tccacccttg agcaaattac
    17041 agaagatacg gtttgtttat ctgtaggcgg tgataattat tgctatgaca actggaggaa
    17101 atggaaagca attcatgaaa gagcaattga agaaggtgca agaagtattt tatggagctg
    17161 ctctatagag ccgtcaatga tggataatga aatgctcgat gtcctaaaat cgcatcatct
    17221 aattactgca agagaatcta ttacatataa tgcactaaaa gatagaggtc ttgacaacat
    17281 caggctttgt tcagatgtag ccttcttatt agagccaaag gaatattccc ttccggaaaa
    17341 tttttttgta ggtaatactg taggaattaa cattagtcca ctcctcataa gaagggaaaa
    17401 gacggaagga atagttataa agaatgtaat aaaactgata aatttggtta ttaacaaaac
    17461 agatatgaat attgcattaa tcccgcatgt tttaatgcct atggataatg accttgaaat
    17521 attaggaaaa atatttaaca gatttaagga tacagggcgt atttgttttg taaataaaaa
    17581 tataagtgca gccgaatata agtatgttat ttcaaattgc cgtcttggtg tgtttgccag
    17641 gacacatgct tctatagcgg catattctac ctttgttccg tctatggtta tcggatattc
    17701 ggtaaaatca aaaggaatag ctaaagattt agggcttgag gattttgttt tgccaattga
    17761 ggaatttcat aatgattgct gtatggtatc aatgttcgaa gaattaatga acaatgaaca
    17821 aaatattaaa aaaatattag ctgataaaat accattatat agaaaaagag caatgccaaa
    17881 ttattaatat actttaggat taagaaaggg agcaggttat gtatataaca caatgtttaa
    17941 tagcaaaaaa tgaagaagct aacatagaac attgtctaag ccatcttaaa acggttgtgg
    18001 atgagcaaat agtcatagac acaggttcaa cggataggac cgtagagctg gcagaaataa
    18061 tgggtgcaaa agtgtttcat tttgactgga taaatgattt tagcgccgcg agaaactttg
    18121 cattagataa agcgaaaggg gactggataa tatttttaga ttgtgacgaa tacttttctg
    18181 aagattctgt tccgctaata agaaaatgta taaaaaaatt tggcagacaa aaggaaatag
    18241 aaggattaac aactaatttt attaatataa aatccaatgg tgaaataatc tctgcagtca
    18301 aaaatgtaag tgcaagaata ttcaaaagaa aaagctatct cagatataag aataaaatcc
    18361 atgaagtatt ggtaaatgtt aataaaacag acgataatat tgtacgtgca gatattagtg
    18421 atttgctaaa aatttatcac accgggtatg ataaaacgat tgtgaatgaa aaaaacaaga
    18481 atgaacggaa ttttcagtta ttagaaaggg agttaaaaaa taatcccaaa gatattaaaa
    18541 ttaattacta tatgagcagg caaatgaaca ttgaaggaaa atatgaaaaa gctattgaat
    18601 atggtttaaa agctatcagc tatatagatg acttctatga atttgattat tattgtacta
    18661 tttatagcgg tataatgata aatatgttat ctttattaaa atcttatgat gagattaaag
    18721 ttatttttga tgacgctata aaaaaatacc ccggataccc tgattattac tggctattag
    18781 gtattgcagc cgtaagagga aataaatacg aagaagctgt aaacttattg gaaaaatgta
    18841 ttaagctatg cgagaaatat aattgtaatg cagaaagttt agcattaggg aaaattgatg
    18901 atgtgtatat agaattgctt aatgcatata atcatcaaaa caataaacat aagattgtaa
    18961 agactgcggt ttctatatta aaggttaata aatacaacat acaggtttta ataattttac
    19021 taaatacctt tttaacacaa gagaaagaag aaaatatagt agctttttta aacaagattt
    19081 atgattataa tttatttaag gataaaatct acttgttcaa agctagtaaa attgttgata
    19141 acataatatt ggttgaattt tttaggaagc tttttaatga tgaggaacta aaaaaaatta
    19201 ataacatcta aaaatatttt tataaaacac taaatatatt tattaggtgg acgatatatg
    19261 ttatgtaaac ataaaaatat tttttatact gaggcgccgg tgtgaaaaat agaatatgta
    19321 aaatataggg aaggatccca agaaaattac acggaggtaa aaaaatgaga attcaacaca
    19381 atttaaacgc aatgaactct ttgcgacaat taggaacaaa taacactaac acaggtaaaa
    19441 acttagaaaa attatcatca ggctttagaa tcaacagagc cggtgatgat gctgcgggct
    19501 tggctatatc tgaaaaaatg agagcacaaa ttaatggtct cgagatggct accaaaaatg
    19561 ctgaagatgg tatttcttta atccagacag cagaaggcgg attaaacgaa actcacgcta
    19621 ttcttcaaag aatgagagaa ctggctgttc aggcagcaaa cggaacgaac caagcagaag
    19681 acttacaaag cattcaagac gaaatagatg ctttagcaga tgaaattgac agaataggcg
    19741 attcaacaaa attcaacggc atcgatttac taaacggtgg cagcatggca tcagatggta
    19801 tagatttcca aattggtgca aataacgcgg actatgaatc tgttaattta actattgcca
    19861 gtatgaaagc atcagactta ggtgtagata ctattgatgt tacagctaca ggtgacccat
    19921 taggtgcaat tgatgctatt gacgcagcaa tagctgacgt atcagcagca agagcaggtc
    19981 ttggtgctat gcaaaacaga ttagagcata caatcaacaa cttgggagtt actaccgaaa
    20041 acttaacagc tgctgaatct cgtatcagag acgttgacat ggctaaagaa atgatggctt
    20101 acactaagaa taacattctt acacaagctg ctcaggctat gcttgcacag gcaaatatgc
    20161 agccgcaggg tgtactgcag ttgctgcagt aaccgttaaa tatattcaaa aaggatgcag
    20221 actcatgcat ccttttttct tgacaacatt tgatttattc atctatgata tacataatat
    20281 taatgtaatt ttaaggtttt tggtataata tagtcataac taaaaatgaa attcgaagaa
    20341 ttgtcgggta accaaacttg gcttaggatg acgagtgtca tattattgga ggtgttgtat
    20401 tgaggatatt aatcgttgaa gatgaagtta aaatcacaca ggcactgagt tatttgttca
    20461 ataagcaaaa aattgatgtg gacattgcaa atgacggtga agaaggactg attcttgcaa
    20521 gcaaggatat ttatgatgta atagtcttag atataatgct tccgggagta gatggcattc
    20581 aaatattaaa aacaatcaga aaagatggaa taaaaacacc cgtaatcatg cttaccgcca
    20641 aggatacggt ggatgacagg gtattcggac tggaatccgg agccgatgat tatcttgtca
    20701 aaccttttgc aacaaaggag ttgattgcca gagtcaaggc gctggcaaga agaaagagca
    20761 cagaatttgt tggtgaaata tatgaatttg aggatattaa gtacgatgtt aaaaattatc
    20821 ttctgtatgt aggagacaat gaatataaaa tacctcttaa agaagcaatg cttatggaaa
    20881 tgttcataaa aaaacctcga caggttttta cgagagagca gattattgat agaatatggg
    20941 gacttgaagc agatatactt gaaagcaata ttgaaattta tgttcatcat ttaagaaaga
    21001 gacttgaaaa taccaatgta aaaattgaaa ctgtaagagg cgtaggttat atgctgaagg
    21061 agaaataatg tttagaaaac ttcatttgaa attaacagca tacatgggca ttatacttat
    21121 tctttttatg ttttttgtgt ccgcaggaat atatcaattc acaaaaatgg tatttgaaga
    21181 cggcaataaa gagctgatga gagcagaagc cctgagaatt tataactata aaaagactgc
    21241 ctatacgcaa tacttcttta acggaacttt atttaaaagg tatatacaaa ttaatccttc
    21301 ggaaaacagg aggcttgatg catgttatgt tgtttatgac caatatatga tacctgttct
    21361 ttccgaggaa ggtgaaataa aaatagcaga ccatattaag ccgcttgcac aaaaagcttt
    21421 tgaagaaaaa aaagattcat atgaaacaca aaaaataggt cgttttaact ataggattta
    21481 tacaagatat tttagtgatt ataccgggca aagcgtagtg caggtttatc aaaacaccgt
    21541 aaacgaggat atattatggt cgttcttaag aacggtactg ataatgatag gaattacggg
    21601 caccgtaata ttactggtat taagctatat gcttacaggc aaggccttag ctcccataaa
    21661 agaaacatgg aaaagacaaa gagaatttgt ggcagatgca tctcatgaac tcagaactcc
    21721 tcttaccgta atacaaacaa acttggatgt tgttttaagc gatgaggacg gcactgttga
    21781 tgaaaatgaa atttggcttg acaatgcata ttccgaaaca agggttatgg ccagacttat
    21841 cgaccagctt ttaattcttg ccaaggctga taacaatgaa gaagaattag atatttccga
    21901 gttttctttt tcggaagtta tagaaaatgt atgccaatcc atggaagtga ttgctgcaaa
    21961 aaagaatatt gagctcataa caaatattga agacaatata ataatcaagg cagattatga
    22021 taagatgcgt agattggctg taattttaat tgacaatgca gttaagtata cagaaaaagg
    22081 caaagtaacg gtaagtttaa aaacagataa aaacaaaaaa gtattgattg ttgaagatac
    22141 cggaatcggc atcaacgaaa aagatttgga aagaatattt gacaggttct atagagcaga
    22201 caagtcaagg cacagggaag gcggtgtagg gcttggattg agcattgcta aatggattgc
    22261 cgacaagcac aagtattcat taacggtaga aagcaccatt aatgtaggaa gcaagtttat
    22321 attaaggatg taataaagca ggagaaattc ctttaaagga atttctcctt tataacattc
    22381 cagtagttaa atgttcccgt tgacagacgg ttaattacct tctttgatgt gcggaaaagc
    22441 acatgttcca tatcatcgta ttcataaatt tccccatctg ctagaatagc tagacagtaa
    22501 tcctctcttc tttccggata aattgcaatt tcaaattcgg ggggaacaat tatgctgcat
    22561 tccaaacatc tgtatgcatt tgacaaaatt ggtgataagg gcgttatttg caaagttttt
    22621 aatgaaggat acaccaaact tcctcctgct gacttattat atgcggagct tcctaagggg
    22681 gaggaaatta ttaaaccgtc gccgcttatt ttttccaggt ggtttgaggc aataaacaca
    22741 tttagatgca gtgttttcat atctattctt ttaagtatga tatcattaac agcataaatt
    22801 ttattggata aatttgcgga aacaatttca cattcaatta aatttacggg atttattttg
    22861 taattgcctg ctctgtaatt aattataaag ttttcaaggt tttcaggtgt aatttccgga
    22921 taaaaaccca aggttccggt atttactcct ataaagggta tttgggaaaa attactttct
    22981 ctcacgcttc ttagaaaggc tccgtcccct cccacagtaa tgtttaattg tgcgtcttcc
    23041 ttgaaacctt cgtaataatc atagccgttt tcggttaaaa ctttaatgag ccttttttta
    23101 atatcagctg tataaacatc atcgttagca gcaatattta ttactttttc cttcattatt
    23161 atccccttta ctttattaat cttatatatt attataagaa cacaagatat aatctaagag
    23221 gtcaaccctc gtcatagctg tgagagcata gtcctcgtca taaaaaaact ccaaggagta
    23281 cataatgttt aacaatatac ttaaatatac aatacaagaa aatgatgaaa ctgtaaagag
    23341 catattgaca gagaaattaa aattctccgg tagactctca agaaaattag agcgtaacgg
    23401 caaaattctg ttaaacggga aagctgtgaa attaaacaag agtatctttg caggtgatga
    23461 attatcgatt gagtttgacg aggaagaaga caggtatgat gctgtggata tgcctttaga
    23521 cattatttat gaggatgatg atttgcttgt tgtcaataaa gagccgcata tggttgttca
    23581 cccgacaaga tctcatcaaa acgacactct tgcaaacggt gttgcatatt atttcaaaaa
    23641 gaacaatatt aaacgaaagg ttagatttat aaacaggctt gatatgaaca catcaggtat
    23701 tgttttaata gccaaaaatc catatatcca caatcagatg gtgctcgaga tgaagtcaag
    23761 catggttgaa aaatattact atgcaatagt ggaagggttg ctagaagaag acgaaggaag
    23821 aatagacgtt ccaattacca gacttaatcc tgaagatata atccggacag ttcatacttc
    23881 aggaaaagaa tgcattactg aatacaaagc agaaaagaga tacaataata tgacattggt
    23941 taagctgaag cttataaccg gaagaacaca ccaaataagg gttcacttaa agcatatagg
    24001 tcatcctatt gttggtgata cattgtatgg aaatgaaagc ttattaatag accggcaggc
    24061 actacactgc tatgaaatga agtttaagca tccgattaca aataaagagc taataataac
    24121 atgtcctatg ccggaagata tgaaaagggt aattgaagga aggtaggagg gaatatgaaa
    24181 aagattttaa tgatagcgac agggggtaca attgcttcaa agataaccga acacggtctg
    24241 gcacctgcta taagctcaga tgaactttta agttatgtgc ctgaaataaa gaaatattgc
    24301 tatgtggata caattcagct tttaaatatt gacagcacta atatacaacc ggaacactgg
    24361 gtcatgatga ctgaaacgat agaggaaaat tatgacaaat atgatggttt tatagtgtct
    24421 cacggtacag atacaatggc atatacagca gcagctcttt cttatttaat tcaaaatcca
    24481 tgcaagccaa tcgttttaac aggctctcaa aaacccataa atgttgatat aaccgatgca
    24541 aagaaaaatc tccttgacag cttcaggttt gttacggaag agggcataag cggtgtatat
    24601 gtggtatttg acggcaaagc gataatcgga acaagggcga gaaaagtaaa atcaaaaagt
    24661 tacagtgctt ttgaaagtat taattttccg gttgcggcaa taatagatga caggcgtatt
    24721 acaaaatata taagtaatta taatttagag aaaaaagtaa agttttataa ggaaatttac
    24781 acaaacatat ttttattgaa attagctccg ggaatggagc cggatgttct tgattttata
    24841 ggcaataaat atgaaggaat agtaattgaa agctatggtg cgggaggact cccttttctt
    24901 gacaaacgta attttttaaa taaacttgaa gaacttataa aaaaagggaa aataattgtt
    24961 gttgctaccc aggtaatgtt tgaaggaagc gacatgggct tatatgaggt aggagtaaag
    25021 gcacttcaaa aatataatgt tctgcaagct tatgacatga ccattgaggc tgtaattacg
    25081 aaattgatgt ggattatggc tcaaaccaaa gattttgaag aagtaaaaac aatgttttat
    25141 accagaatac acgaggattc tctatactaa gaaaggaata tatggtagct gcttgctata
    25201 tcaaacaaga caaatatgaa gaaggttgac ttgcacatac acaccaatgc ttctgatggc
    25261 acttgggatg tgacagaatt aaaaaatgaa ttgaaaaaaa ataaaattga catattttcc
    25321 gttacggacc atgacagtgt tgaaaacatc gaaaatatgc aaaatatatt aaaaaataag
    25381 gacaatttaa tttttattcc cggaaccgaa ctatccgctg aatataaagg gaaagaatat
    25441 catttgaatt tatacaatta tgatataaaa aacaaagaac tgcttgagtt aatgaagtgg
    25501 acaaatcaaa acaaattaaa ctcaaatgtt gattatataa attatgctgc aaataaatat
    25561 aaaaatatta gtgttgaaga tttcgaaaga tatgcgaatg acagaaaaag agggggctgg
    25621 aagtcagcca actacatgat tgataaagga atacacaaag atatccccga acacttgaag
    25681 gatgtttatg aatcgggact taaggctgaa ttgaaaagcc cggaggaagt aataaacata
    25741 gcgaagaaaa gcggagggaa aatattcctt gcacatccat cctatcacta tagggattct
    25801 gttatgccca tagaagaatt aaaacactgg ggggagcttg gaatagacgg aattgagtgt
    25861 tattctcctt ataacaggga caatacccga tattatattg atttttgcaa aaaaaacaat
    25921 ttaatgatat caggcggctc cgattgccac ggaagcttta taccttccag gaaattagga
    25981 gttcccgatg tttctttgga tgatttgaat attaaaaaat tattatgatt agccaatgcc
    26041 ccgtttggag gaagatatgc aaaacaaaaa acatatttct gcaataatag ttgcagccgg
    26101 aaaaggaaaa aggatggact ctgatattcc aaagcaatat atggatattg caggaaaaac
    26161 aattttggat accaccttgt ataaatttga aaaaagcaat gatgtagatg atattattct
    26221 ggttgttaat aaggaggata tagagttcgt taaagatgaa atcgctcctt tttaccgtaa
    26281 aatagcatat gtggtaccgg gcgggaaaac aaggactgaa tcggtttatc agggcataaa
    26341 aaaggtcagt aaaaactgta atatagtctt aatccacgat ggagtaagac ccttcgtttc
    26401 ctataattta ataagcacat gcgttgaaaa tgctataatt cataaagctt gcgtaccggt
    26461 gatagacgtt atggacacaa taaaagaagt atcggaagac ggaatggttg taaaaacatt
    26521 tgacagaaaa aaattgaggg ctgtacagac acctcaggct tttgattatg acataatcag
    26581 ggaatgctat gaaaaagcca tgactgaaga aaaagaattt acggatgatg catccattgt
    26641 ggaatactac ggttataaag tgaaaacaat tgaaggatta tataagaata ttaaaataac
    26701 cacacctctc gatttgagaa tggcagaaat tattgttaac tttttttaaa ggagctgcta
    26761 tgaaaatagg aataggatac gatgtacatg ctctggctga aaacaggaaa ttaataatag
    26821 gaggggttaa aatcccttat gaaaaaggtc ttgacggtca ctccgatgca gatgttttaa
    26881 ttcatgcaat tatggacagc cttttgggag ccatgggaaa aggagacata ggaaggcttt
    26941 ttcctgatga tgatgctaag tataaggatg cagacagtcg cgttttatta agaaaagtat
    27001 atgaaataat gagggaaaat aaatttacgg tcggtaatat agatgccgtt attattgctc
    27061 aaaaccccaa attatctcca tttattgatg aaatgaggcg taatatcgcg agtgatttaa
    27121 aaactgatat ttctagtata aacattaagg caaccaccac cgaaaccctg ggctttatag
    27181 gaaggggcga gggtattggg gcagagtgca tatgtttgtt ggaaacaaag gaaaaataat
    27241 gaatccaagc tatttattga gtatatataa aagtatatcc ccgtgttata atttaataaa
    27301 gaaaaaaata attatgggac acgcttcaat atggggtgac tctctgaggg atagatagag
    27361 gaagggggac acacccttaa ggagggtaag tctctgaggt cagaccttaa gggaatgtcc
    27421 ccgtacttgg aatgtcccgt acttgggggg attaatatgg aaacatatca gattatatca
    27481 attattggcg ttataatttt tatatatttc atatttttta aaaatcgacg ctctgcacaa
    27541 aaagaaataa gcagcttaaa tgaaattgaa tatataggct catcaactat gaaatttgat
    27601 gatgtggccg gaaactatga agcaaaacaa agtcttcttg aactggtaga cttcatacaa
    27661 aatcctgaaa aatacagcaa gtttaatgta aggatgccaa aaggggtact tctttacggc
    27721 gaaccgggaa cagggaagac tttaatggca aaggccttgg caggtgaagc cggggtacct
    27781 ttctatgcag tatcgggttc tgattttgtg caagtttatg caggtcttgg agccggaaga
    27841 ataagaaatt tattcaaaaa agctaaaaaa tcaggcaaaa gtgttatttt tatagatgaa
    27901 atagatgcca taggaaagaa aagaatgtca ggtagcatta aaggaagcga tgaaggtgac
    27961 aggaccttaa atgcacttct aaccgagatg tcgggttttg ccgatgattc aggaactgta
    28021 gtaattgcag ccacaaacag aattgatact ttggacagtg cattgttaag accgggaaga
    28081 tttgacagac aaattgaaat aatgcttccg gacaaaaatg caagaaaaga tattataaag
    28141 ctttatttgg acaaaaaaaa tgcagaagat attaatatag acgagctggc tgatttgact
    28201 gtttatttca gcggagcaat gattgaaaat ttaataaatg aagcagcact tttggcaata
    28261 agacaaaatt cagataaaat taaggaagaa catatcaaaa aagcgtattt gcaaatattg
    28321 gcgggagttg aaaaagagga ttatgataat ggatttcaaa aaaccattac atcatatcac
    28381 gaagcaggac atgcatttgt ttcaagacat ttgctgcctg aaaataaaat tattcgagtt
    28441 tctgtaattc ccaccagtaa gggagccgga ggatacacgc tgagcactcc tgactcggaa
    28501 gaggtaatga ccaaagggaa aatgataaat catttatctg ttctaatggc ggggagagca
    28561 tctgaagaag tattcttcgg gaatgaaaat attaccgccg gagcacaaaa tgacattgaa
    28621 aaagtaacct ctttggcagt ggattatgtt tcaaaatacg gcatgaatga tgcaacattc
    28681 ataaattatc agctgctgtc cgataaactg agaataccat tgacatcagt tgaagtctcg
    28741 gtaaaaaaat tgacttcaga aatatatgaa gaggtgatta ggttaattga atgcaataag
    28801 gaaaaagttg agaaaatagc aagactgtta atggaaaaag aagttattta ccaggatgaa
    28861 ttggataatt tgatttaaaa atatagttga atattatcta aaacacaggt taaaatacta
    28921 gtgaataaag cagccttcat aaaaaccggt tgtaattatt ctaaaaattt gatataatta
    28981 aattaaagat agcctgcggt gttcggaaat cttttaattc aaccgacata ttgtaaaagg
    29041 gaattcgcgt ttgactgcct atgacaagcc cgctttgacg ggacgccaga atcagccaac
    29101 agaaatccca cctgcctagg tgcgggtgtg aattataggt ttgcggcatt gcgggtatta
    29161 ttatgttatg aataaatata gatttttcat actttaattt gtcattctga gaggggcacg
    29221 tagaatctca ctgcatactg agatttttac tgccgctcag gatgacattt ttttaggaaa
    29281 acgggaaaat aacatttgcc tttatcatga aattcatata taatggtaac aaaataggaa
    29341 tacattacca atgattatag gaggaatata tgataacgat caaagacgtc gccaggcttg
    29401 cagaggtatc gatttcaacc gtatcaagag tgataaacga ctcaaagcct gttagtcccg
    29461 aagttaggaa gagggtttta aaagttattg aagaaaccgg atataagccc aatgatgttg
    29521 cgagaagcct tgtaacaaga agatcatatt taataggtgt tatagtgaat aatttagcca
    29581 acagctacgt tgcagacact gtgagaggaa ttgaagaaat aggaaaaatg tacggatatg
    29641 atattcttct ttgcagcagc tatggcagca aggaaactca ggaaaaatat ctgcagctgc
    29701 tagacagaaa gcaagccgaa ggtttgttcc ttgtaggaag tatatttgat gaagaaatta
    29761 ttaaattggc taaaagcttt aataaaccct gcgtattttt tacaagagat attcgcgaaa
    29821 acatgaatta tataacaatt gactgcaaat ctgcaattta tgaagtaaca aaatatttat
    29881 tcgaagaagg ccacagaaaa attgcatatt tttccgacta tgaaaacaga actaccgtag
    29941 aagacgaaaa ggttgaagga tacttaaaag caattgaaga taatgaaatg gaatattcta
    30001 aaatatatgt tgtcggagga agcaagcaca atgaagcata tgatttaggg aaatcaataa
    30061 ttgatgaagc aaaggatttt actgcatgtg tttgcagctc tgatgaaata gcaataggga
    30121 tattggacag ctttttagac aacggaatta aagttccgga cgatgtgtct attgtaggat
    30181 ttggaaatat tagggaggga aaattcgtga gacctgaact gactacaata ggtgagcctt
    30241 attatgatgt tggagcggta ggtatgagaa tgcttataaa aatgataaaa ggcgataaaa
    30301 ttcaacaagg acgtatggag ctgccattta ccatagaaaa aagaaaaagt gtaaaaaatt
    30361 tgaataaata atacaaagtt tgttgaaatt aaacgagtaa tttaattttg agcgcgacaa
    30421 ttattgataa aataatcaaa gtaatacccc tataatgaaa agacatcttt attgcagggg
    30481 gattacaaat gaaaaagaga tttattttca cgatgatcgt gttgctgctt ttcagttaca
    30541 attcaattaa cggctatgaa tatcataaag aaatagagac tattgagtat ttgctaaatg
    30601 agcggataac aataatgaat gaatttctct acggatataa agatatgcaa agcttggagg
    30661 agaagttaag caaaattgaa tccgataatt tactgcaaaa tgacttggat attttatata
    30721 aggtaaagaa cagccctaca gattatgaat tggcaatgaa ggttaaggtt aatgaaattt
    30781 atgatataga gcttaccgat gacggatatt tggtaaatgc tgacctgaat tggcttatga
    30841 gaggatatga cggtgaattt aatttgatta gaaattatca aattaaatgc atagaagatg
    30901 gcgggcaatc atatttggca aaattaacgg tattaaacaa tacagcagaa taatttaata
    30961 tttcaaaaat aatgaacaag tattttaata caaaagaatc ctcatagtga ggattctttt
    31021 gcttataaat tatcaaaacc ttaaataaaa atcaacaata ttttgacttt ttatgttgat
    31081 aattgtataa ttaatgttat agttattttt aaaataaatt ggaggatggc aaattgcaaa
    31141 atgagttttt acaatcaaaa aaaagtgaat ttgccgaatt gaaagagtat atcgattcgg
    31201 taaaaaactc acaaggcgtt accatgcaaa tattgcagaa agcacaaagt ttatttggat
    31261 acttgcctct tgaagttcaa cagtttatat cagatgaaac aaatatacca ctggccgata
    31321 tttatggagt tgtaaccttt tacacacagt tttccacgga agaaaagggt aaacacaaaa
    31381 ttggattatg tcttggtaca gcttgttacg taaagggctc gcagggcatt ttagacaaga
    31441 tttctgatga attaaatgtt aaagtcggga aaacaacgga cgataagctg tttaccttag
    31501 aagctacccg atgcttgggc tgctgcggat tggccccggt tatgatggtt gacgatgatg
    31561 tttacggcaa attggataca acaaaaatcc ccggtattct aaaaaaatat aaataggagg
    31621 gagcagcatg aagtcgttgg aagaattaag aaaaataaga gaagaagcaa taaaaaatat
    31681 tgaaatgcga aatcccgata aagatataag agtggttgta ggtatggcca catgcggaat
    31741 ttcagctggt gccagacctg ttctttctac attcgtagac gaagtagcaa aaagaaattt
    31801 aaaaaatgta caagttgttc aaacaggctg tataggcatg tgtacgtatg agccgattgt
    31861 agaaatatat tcccctgata aggaaaaggt gacatatatt catgttgact ctgaaaaagc
    31921 aaaaagaata gttgcagagc acttggttaa tggaagagtt gtaagagaat atacaatagg
    31981 ttcggaaatt tagattaatg aaaatggagg taaaaccatg gcaattttca gagctcatgt
    32041 tttaatatgc gggggaagcg gatgtacttc taataaatct caagtagtca gaaaaagatt
    32101 tgaagagaaa ataaaagaag tacaattaga caaagaagtc caggttgtgg gaacaggctg
    32161 ctttggtctt tgcgaagaag gtcccgtagt catagtttat ccggaaggct cattttattc
    32221 aagaatgact gtggacaagg ttgacgaaat agtagaagag cacttgttaa agggaaggat
    32281 agtcgaaaaa tatttatata aagaaaaggt aaaggtagaa gatgccaaag agcataaatc
    32341 cttaatggaa gttcagtttt acagcaagca aaaaagagtt gcactcagga actgcggttt
    32401 aattaatcct gaggatatta atgaatatat agcaagagac ggctatatgg cattaggaaa
    32461 agtattgact gaaatgtccc cccaagatgt aatacaatta atgaaagaat ccaaccttcg
    32521 aggaagaggg ggggcaggct tccctgtagg attaaaatgg ggctttgcag caccaaataa
    32581 agccgaccaa aaatatataa tatgcaatgc tgacgaagga gatcccggtg catttatgga
    32641 cagaagtatt ttagagggag accctcatgc agtattggaa gccatggcta tagcaggtta
    32701 tgcaatcgga gctacaaagg gatttattta tgtcagggca gaatatccca tagctgttcg
    32761 cagactgcat atagcaatcg accaagcaag agagcttgga ttattaggta aaaatatatt
    32821 tgattcagga tttgattttg atatagaaac aagacttgga gccggtgcat ttgtatgcgg
    32881 agaagaaaca gcactgatgc agtcaattca aggaaacagg ggtatggcga aggtaaagcc
    32941 tccattccct gctaacaaag gcttatgggg aaaaccgacg gtaataaata atgttgaaac
    33001 cttagctaac ataccgcaga ttatattaaa aggacctgaa tggtttaaaa gctttggtac
    33061 ggaaaaatca cccggaacaa aagtatttgc attgggcgga aaaattacta ataccggact
    33121 ggttgaagta ccgatgggaa taacattaag agaagtaata tatgacatcg gaggaggatg
    33181 ccccggcggg aaagcattta aagcagttca aacaggagga ccttccggtg gttgcttgac
    33241 agccggccat cttgatactc ccgttgacta tgaaaatctt gcagctgtag gctcaataat
    33301 gggctcaggc ggaatgatag taatggatga agataactgt atggttgaca ttgcaagatt
    33361 cttcctggat ttcattgttg atgaatcatg cggcaaatgt acaccttgca gagtgggaac
    33421 aaaaagaatg ctcaccatac ttgaaagaat tactcaaggt aagggaacct tggaagattt
    33481 agaagaactc gaaagcttgg caaacagtat aaagaattca tcgctctgcg gtcttggaca
    33541 aacagctcca aaccctgtat tggcaacact cagatatttt agagatgaat atattgcaca
    33601 cgtggtggat aagaaatgtc ccgctaaaaa ttgcaaagct ttgttaactt ttgaaatcga
    33661 caaggataaa tgtacgggat gcactctttg tgcaagaaat tgtcctgtaa gttgtatttc
    33721 aggaactgtt aagaatcctc atgaaataaa tcaagaatta tgtattaaat gcggaaattg
    33781 ctacagcgta tgcagatttg gcgctgtaca aaaaaattaa aagggaggat tagccgtggc
    33841 cttagtaaaa cttaatataa atggaaaaga acttacagct gaagccggga aaacagtact
    33901 ggaagcagct ttagaaaatg gcatagaaat tccgcacttg tgttttgatg aaagattgga
    33961 agtgtatgca ggatgcggac tatgcatcgt ggaaatcgaa ggacagccaa aaattgcaag
    34021 agcttgtgca actcccgttt ctgacggatt agttgtgaga tcagacactc aaaaagtaat
    34081 agaagcaagg aataatgcgc ttaatttatt ggtttcacag catataggag actgtaaggc
    34141 accttgtacg ctaaactgtc cggcaaatac tgatgtacag ggatatgtcg gtttagttgc
    34201 aaataaacaa tatattgaat cattaaaact gataaaagaa aaattacctt tgcctgcatg
    34261 cataggaaga gtatgccctc atccttgtga aaaagcatgc cgaagacaat atgtggaaga
    34321 gccggtatca atagcgtgta ttaaaacatt tgcagcggat tacgacttga atacaggcaa
    34381 tccctatatt cctgaattaa agcctgctac aggcaagaag gttgctgttg tgggagcagg
    34441 tcctgcagga ttgtcagcag catactatct gttggcggac ggacatgaag tagatatatt
    34501 tgaagcaatg ccaaaacccg gcggaatgct tagatacggt attcctgaat acaggcttcc
    34561 gaaaaatgta gttgatgcag aagttgcaat tatcgaaaaa atgggtgcaa atttcaacta
    34621 cggcgtaaaa gtcggcgaag acatttcttt ggaatatttg caaaataaat acgatgcagt
    34681 gttccttggc ataggagcat ggaaaagttc acctatgaga tgtgaggggg aaaatactcc
    34741 gggagtatta ggcggaattg atttcttaat aaaagtagca gaaaacaagc cggtaaaaat
    34801 aggcgaaaaa gtaatagttg taggcggcgg caacactgcc atggacgttg caagaaccag
    34861 catacgttta ggagcaaaag aagtaagagt agtttacaga agaagtgaag aacaaatgcc
    34921 cgccgaagaa atagaaatta aagaagcgaa agaagaagga gtaatattct cattcttgtc
    34981 agctccgtca ctggttttaa gcgacggtga aaaagtaacc ggacttaaat gcgaaaagat
    35041 ggtccttggt gaaaaggatg catccggaag acaaagtccc cttccaaccg gcgaatttgt
    35101 agaattcgaa gcagatacga ttatagcggc aataggtcag gaagttgact gcggtaaaat
    35161 taatgtagga cagggaaaaa agaaaaatat agttataaat gaaggcactt ttgaaacaaa
    35221 tttaaaaggt gtgtttgcag gaggagatgc agcaacagga cctaagattg ccatagatgc
    35281 agttgcacaa ggcggacgtg cggcagcagt tatttcaagt tatctggcag gtgaaatgat
    35341 tccttacaaa aaccagccat tagtatatac tgaagtaacg aaagaagatt ataaagacga
    35401 agaaagaatt ccaagagttc ctcacagaac tgtagagccg gaaataagaa agcataactt
    35461 ccagcccata cttccaacaa tgacagagaa agaagcaata agagaaggac aaagatgtct
    35521 tgaatgcggt tgtaaggatt actttgaatg ccagttggtt gactatatca agaagtttga
    35581 agttgatacc aaaaaatatc atgcagaaaa tgaaaagaaa ttcaaagaaa cagaccatcc
    35641 cttcatttcg caaaatgttg ataaatgtgt actttgcgga ttatgtgtgc gtgcatgcga
    35701 cgaagttgtg ggagcatcag cattaggatt tgtggaaaga ggaaatgcaa catttatagc
    35761 acctgcattc gaacaggaat taaaagtaac aagctgtatt tcatgcggcc agtgtattga
    35821 tgtgtgtcct gtaggagctt ggcttgacag aagaggcaat atgaaggaaa ttcctctgga
    35881 cttaacacaa accaagtcag tgtgcggcta ttgctcagta gggtgcgatg ttgtatatga
    35941 gcacaaagac aatatggttt acttggcatc aagtcctaaa gaaaatgaaa tacccgtatg
    36001 cggtaagggt aaattcggta tcgagcatat taacgatgaa aacagaatcc tccagtcaaa
    36061 ggttaaggta aaaggaaagt atgagccaat agaattacct gttgctttat ttgaaacggc
    36121 taaacgctta agaagcatac agaatatgta cggagatgac caagtagcat ttgttgtatc
    36181 accaaaatta acaaacgaag aatttaaata tattaaagct gtggcagatg aattaaatac
    36241 taaatacaag ggttcattta cacttgattc ggaatccggt attgaatatg tattaggcaa
    36301 gaatgaatct accataagag cagaagaatt ggacaatgca gatttgatta tttctgcagg
    36361 tcagttgtac gagcatcacc ctgcagcagg aatgaaatta agaagacttt ctaacactaa
    36421 gaaaataatt tctgcatcaa ctattaaaac taagcttaat aactttgctg ttacagcaga
    36481 cgtaacagat tatgaagtgt tctttacaag agtactgaaa gcattggttg acaacggagt
    36541 tattaagaaa gaagcaataa gccgctatga aaacggagaa gagctgatta aggcattgga
    36601 caaaaccgaa gtacttcctg aagcagctaa ggttgcagaa gctttcaaga aggccagaaa
    36661 acccattatc gttgcggatg aaaacacagt tccaaaggca gcactgaaag tcctggctga
    36721 tataaccgta ttagcaggaa ataataaccg tcctcacaga ggcttgataa ctcttaaagc
    36781 taaagcaaac agccagggag cttggaatat tggtttcagg acaccgggag aagaaatcag
    36841 gaaagcattg ttaaacaatg agcttaaagg cttagttgta atcggcgaag atatattggg
    36901 aaatgttcct gaacttaaga aagcagcaga caatctaaaa ttctttgcag cctttgacat
    36961 aatggaaaat gaaactacct ctaatgccga atatgtactt ccgctttgca gcatagctga
    37021 aagcaatggt accatagtaa gcgcagacgg gaccgtaaga aatgtatatc aagcaataaa
    37081 acctttaaca ggcacgtcaa accttgaaat gttcgcaaaa ttagccggac tgttaaatgc
    37141 agaatacaaa gaggatgcta aggacgaagc aaaagttaag ctttatgtac ctacgctgaa
    37201 aggcaaggaa aaagaatacg aagtatacga cacggtagaa aaagcattcc ttgcaaagct
    37261 aaaagaaaac tgcataaaag cagtttaatg acaaggggac gggggtactg tcacaaaccc
    37321 accggggacg gttctcgctg gacaaattcc tcttacccac tggggacggt tctcgctgga
    37381 caaattcctc ttaagaaaac attaatattt ggttgttaaa aaatgtttat ataagttagc
    37441 cttgcaattc caacgttatt ttaattatct acccctttgg ctaaaagaat ttatttcttt
    37501 gcatgtgtta tatatcctcg attgcaaggt ctttccattg attccatata agatattgac
    37561 tacaattttt acaaccgtat ttcttaaaga gtacctgggc aggattctgg aagacataag
    37621 gaggataaaa tgaaaaaaat ttttaagtca atattattaa cagcaagtct gactatcgga
    37681 ctatccatgt tcgtagctgc tgatacatat gcagcaacct acacaatagc taaaggtgat
    37741 tctttgtaca aaataagcaa agcttacaac acaaccgtat caaatataat ggttgcaaac
    37801 gatttaaaaa gctatgattt aagtatcggt caagtaatta atattccgga cgtaaaatac
    37861 atagtgaagc cgggtgatac attatataaa atatcacaaa aatataatat tccactgttc
    37921 aatttaagac aagccaacaa catttggact gattacatat atgttggaca ggttttggag
    37981 attccgttgc caccggcagc agtaaaagaa gaacttaagg ttgcaaaaca gccgaacgaa
    38041 ggatataaag actatactgc cgaagaattg gacttgcttg caagattaat aatggcggaa
    38101 acagagagcc agtcttatga agctaaggtt gcagtcggct ctgttgttat gaacagggta
    38161 aaaagcggtt tgtttgcacc gacaataagc ggtgtaatca accaaaacat caatggatat
    38221 taccaattta cccctgttga aaacggttgg attaaaagag ctgccaatga agaatgtatc
    38281 aaagctgcaa aagaagcact aagcggagtc gatactacaa acggtgcatt gttctactat
    38341 gacgattcaa caacaaatca atggatttta tcgaagccgg tttctgttaa atacggaaga
    38401 atgatatttg catattaata aattaaaaac agaggaagat taatttcctt tgctttttta
    38461 ttttatcaga agagcagatg caactccaaa ataaaccaag agtaccaatg catctacaat
    38521 tgttgtaatt aatggtcctg ccattatggc agggtctaat ttaagctttt ttgctataag
    38581 cggaagggtc ccgccaacca ttttagctaa cataactgta gttgccacag ttaaggatac
    38641 agttaaagaa acatttaaac ctaccttgtc aaacagcata ttttttgcaa aatttactga
    38701 tgctaatata attccaacca ataagcttat tctgaattcc ttccataaaa ccttccaaat
    38761 gtctttttgg ttcacatttc ctaatgctaa cccacgaatt attaatgttg aagattggtt
    38821 tcctgagttt cctccggtat ccataagcat tggaataaat gcggtaagaa caacaacagt
    38881 gctgaggata ttttcaaatt ttgtaataat gccgcctgta acggtagccc caagcattaa
    38941 tatcataagc catggaatcc tttgttttgc tagtgctatt acacttaaat ccaaatacgc
    39001 ttcatccgat ggtaccatgg ctgccattct ttggaagtct tctgtagctt catcttccat
    39061 tacatccatt atatcatcaa acgtaataat acctattaat ctgtgctcct tatcaacaac
    39121 aggcaatgcc ataaaaccgt attttttaaa aatatttgct attatttcct gatcatcgtg
    39181 agtttctaca taaataactt cttcttgcat aatatcgtta atgctttgat caggatcaga
    39241 aactaccaga tttcttaatg aaactattcc ttctaatttt cttttcttgt ccgtaacata
    39301 acatgtatat attgtttcct tgtttatccc tgttttttta atatgttcaa gagcttcctt
    39361 aactgtcatg tttttcttaa gctcaacata ttcaattgtc attatgcttc ctgccgaatg
    39421 agcaggatat tttaaaaatt gattaattaa agttctttct tcctgagttg aattgattaa
    39481 aattttattg acaacattag caggcatttc ttcaattaag tctatcatgt catcaaaaaa
    39541 cagctcatcc aaaatatatt ttaattcttt gtctgtcacc gcattaataa tatctgcttt
    39601 ttgttccggt gaaatataag aaaagacttc tgcagccgta tcctttggaa gcatcctgaa
    39661 aacgagcaag gttgtttgga ggtctaaagg atctaatatt tctgaaatat ctacttcatt
    39721 catttcagaa agttcttttt ttaactgaat atattttttt tgctcaatta gttctaaaat
    39781 atct
//
LOCUS       DAOSHP010000006        39768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_778_length_39768_cov_8.639740, whole genome shotgun sequence.
ACCESSION   DAOSHP010000006 DAOSHP010000000
VERSION     DAOSHP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 39768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39768
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_778_length_39768_cov_8.639740"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..352
                     /locus_tag="PLB33_01305"
     CDS             77..352
                     /locus_tag="PLB33_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOG62187.1"
                     /translation="MPRRDGTGPIGMGPMTGRGFGFCGHRFYGRRNCFGRGFGPWYGA
                     GYGFAPGYGQGYFKQDMTEKEFLKNQKEFLESQIELLEKELEDLKDD"
     gene            369..734
                     /locus_tag="PLB33_01310"
     CDS             369..734
                     /locus_tag="PLB33_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG62188.1"
                     /translation="MKIAIPTEEKSIKSNIYDYLGRAPYFLIYSTVTKEYEFLDNRAV
                     VEQGGAGIRAAQVIADNGVKAVITNRCGENAEKLFRNAEVFIYAAQKGTAEENIAYFN
                     ENKLSLLNEFHSSFHGKEK"
     gene            803..1156
                     /locus_tag="PLB33_01315"
     CDS             803..1156
                     /locus_tag="PLB33_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG62189.1"
                     /translation="MRIAIPVDDGSVCVSFGRTPYFMLYDTENNESSFINNSAANSQG
                     GAGIKAAQIIVDNNADILITPRCGQNAAEVLTAANVKIYKALNDSIEENLTAFKNDKL
                     KILTEIHSGFHGGEK"
     gene            1156..1989
                     /locus_tag="PLB33_01320"
     CDS             1156..1989
                     /locus_tag="PLB33_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62190.1"
                     /translation="MMIAVLSGKGGTGKTFVSVNLSFVADNSNYIDCDVEEPNGHLFF
                     KPNIIKSEKVHVKIPNINNELCNGCRRCVDFCKFNALAYIKDKVYVFDEICHSCGGCM
                     LLCPEKAISEKDKEIGEIQEGIFKNSNIYTGILNPGEETGVSIIKKLLKIAGNKPYTF
                     IDCPPGSSCSVMESIKDVDYCILVAEPTLFGVHNFNMVYNLVKLFDKPFGVVLNKCLD
                     GENPAEKYCNEKNIKVLCKIPFEHELGNINSNAEIASEKNEKYKSLFSSLLKTIKEEV
                     K"
     gene            1986..2837
                     /locus_tag="PLB33_01325"
     CDS             1986..2837
                     /locus_tag="PLB33_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62191.1"
                     /translation="MKQLLILSGKGGTGKTTVAGAIIKLSNVKAYADCDIDAPNLHLI
                     MQQESIPENSDYYGLDKAVIDKDKCLMCNLCYDSCRFDAISDYTVSLYDCEGCGVCEA
                     VCPASAINLMPYKAGDMTLYRGNQVFSTAELKMGSGNSGMLVTEVKKNMRFAAADADF
                     AIIDGSPGIGCPVIASISGVTMVLVVAEPSLSGISDMKRIIETAKKFNTKLAVCVNKY
                     DTNIANNEKIKRFCQDNKIDFVGEIPFDKKAVEAVNNGLTIVDIDCAAGEAVRKIYKK
                     TMKILFN"
     gene            2847..3683
                     /locus_tag="PLB33_01330"
     CDS             2847..3683
                     /locus_tag="PLB33_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOG62192.1"
                     /translation="MSENCNESCSSCSKECDSRKEEQTNFLERPHPQSNIKKVIGVIS
                     GKGGVGKSLVTSLLAVTMKRRNHNVAILDADITGPSIPKLFGITEKARGGSNGVLPVK
                     SKTGIEVMSINLLLENTTDPVVWRGPIIAGTVKQFWTDVIWKDIDFMFIDMPPGTGDV
                     PLTVFQSIPVDGIIVLTSPQELVSMIVEKAVKMAESMNIPIIGIVENMSYFKCPDCNK
                     EYKIFGDSKIEDVAKKHGLNVIAKLPIDPKISSMCDKGLIELYGDEPFTGIAEFLEEM
                     EG"
     gene            3685..4038
                     /locus_tag="PLB33_01335"
     CDS             3685..4038
                     /locus_tag="PLB33_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOG62193.1"
                     /translation="MKIAVASEKEIVTEHFGHCENFNIYETDGNKIVKSESVKNPGHR
                     PGFLPNFLNDIGVNVIISGGMGQGAIDIFNEKGIEVITGALGEAKIAAEEYLRGNLKS
                     TGYICHEHQHHGECE"
     gene            4348..5601
                     /locus_tag="PLB33_01340"
     CDS             4348..5601
                     /locus_tag="PLB33_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG62194.1"
                     /translation="MVLDSDRVWKIVILIICLVLSALFSASETALMSLSKIRVKQMIE
                     NREKGANRINKLLSDPSRLLSAILIGNNVVNIGASSLMTSLAIDAFGNTGVGVATGIM
                     TLLILVFGEITPKSLAAKNSEKISVRLSGFIEFVTNLLTPISFVLNIITDFLVKLLGG
                     EVDKKKPFITQEELKTIVNVSYKEGVLEGEEKDMIYNVFDFSDSQVNDVMVPRTEIVA
                     IDVDLPYEEIIKIINKEQYSRIPVYENTIDNIIGILYVKDLLFLDVNKESPFDLRKYI
                     RQPYFTPEYKSIKELFKEMRTNRNHMVVIIDEYGGTEGIVTIEDVVEEIVGDIEDEYD
                     KKIKEIEVIKEDEYLVNGNVRIDTINELIGTHIESKDFDTIAGFVIGIIDRLPEAGEE
                     IEYENIRFIIENIDRNRIKKIRILT"
     gene            5738..8257
                     /locus_tag="PLB33_01345"
     CDS             5738..8257
                     /locus_tag="PLB33_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62195.1"
                     /translation="MKKGTGFFHRSISLIVLFGVLICLPASALSFIPKFETESINFDG
                     TQSSWAESELKEAYENGLTYGQIENNYQKPITREEFCVIVVKLYEKLGGKAPVMGTNP
                     FTDTDNAEILKAYALGIVKGTSDTTFTPANNITRQEICVMILRALKAAKPGIDTSLGS
                     DFPFQDSSSIASWALDAMKFAYKNDIMKGTSATTISPLNNTNREQAIVLLKRTFTKYS
                     EAPEAQGQGDPNEYYSSLLNGRAPSLNKNVFKPFVPANDSGSSSYSQFTAGTALSGGI
                     VISPTGPGMDIIDSLKEKPGSDLRLLTPEEIEKYWDEYYDFPDESDYEPSVDWVKASD
                     NVLGVDCLGKGYDVIKGKFADPYSLKDSVLSTNKLLEAKRVFKTDGRQSVSRFTEGKS
                     LYSYSKEMARKIGVSGGYLYFSGSINASFGSQTYTETNSRYATLVSDLSQYLIEFDDN
                     YLDYSKYYDSSFKKDIEDDSVTPEEIFNRYGTHVIRAVKMGGRLDYNVQANSSYHSGS
                     HTFESEVKASFNMAFASAGASHSTSQKETTTSFNENCEINIKAYPDYLGSNTITPSQY
                     TAWGEYVRKYPAMCDFGEDTPLIPIYNLARNPFRRYELKKAYEKYAEENQYIPTAAIT
                     CINGIRLNPQPVGRTLDGLDEQVIIDPITNDKWYLVANITAHDFYRDEIQQQIYIRTG
                     FSDLASRPPVVAVFLVNESQGENAKEIFKKYWGNDPTAKLWGDGGEAKSDPSLKNEIS
                     SYADRLKLYYVTSTKGRPITALRVKHFGYDNLIRYYPPKDTNSPHDDYGKFFSVIDYG
                     THMKQGINENQNCAEGAVPLPFGPAHQNIYLQYSHDYSISY"
     gene            complement(8426..8830)
                     /locus_tag="PLB33_01350"
     CDS             complement(8426..8830)
                     /locus_tag="PLB33_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOG62196.1"
                     /translation="MKRLLIILFIIITLTITITGCTSMEKNKTGTETAYKKISAEEAK
                     AIIDSEDVIILDVRTQEEYNEGHIENSVLLPVNDITGKAGEILADKDAKILVYCRTGN
                     RSATAAKQLIKMGYTNVYDFGGINSWPYGTVK"
     gene            complement(8863..9573)
                     /locus_tag="PLB33_01355"
     CDS             complement(8863..9573)
                     /locus_tag="PLB33_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOG62197.1"
                     /translation="MITYNKHIEYLHELKRKIMLHRHRLTIIMMLVILLGTMASGCAI
                     GETDTTSPNTPAGESIVVTITDPPDELEPIVVPTMPEVIPPYLGADPETGLTMTGTPT
                     LVDFDTYRLKVSGKVDQELSLTYDDLRRMPKLTATPTLECVGYFKDVATWSGASLKTI
                     LEMAKVQPGSTRVAFKGADGYEISMILENALAPENFLAYELEGKTLPVLQGFPLRVVI
                     PSYDGSVWVKWLLEIVVE"
     gene            9783..10574
                     /locus_tag="PLB33_01360"
     CDS             9783..10574
                     /locus_tag="PLB33_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG62198.1"
                     /translation="MDKQTIARLAAEFVQKEINYVSNDKALNEELAGLKIFDEPIFAY
                     GAADDEGFQLLKSPSVIGEHFMMPKEWLQSANTVIVYFFPFTDEIREGNRTDMSWPSN
                     EWLHGRIDGQIFMNEFNLFLNSKLNEEGYKSLVPGLDSRFKSEGFSSNWSERHVAFIC
                     GLGTFGLSKGIITQKGMAGRLGSIVTELKLKSDIKKYNNIYEYCINCGICAENCPAGA
                     ISLENGKDHKKCSDFLDKVKEKHKPYYGCGKCQVCVPCENENPKD"
     gene            10646..10951
                     /locus_tag="PLB33_01365"
                     /pseudo
     CDS             10646..10951
                     /locus_tag="PLB33_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
     misc_binding    11068..11264
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11335..13746
                     /gene="leuS"
                     /locus_tag="PLB33_01370"
     CDS             11335..13746
                     /gene="leuS"
                     /locus_tag="PLB33_01370"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOG62199.1"
                     /translation="MKSYNPQAIEKKWQEIWRKNETFKAKDNTDQKKFYGLVEFPYPS
                     GVGLHVGHIRAYTSLEVISRKRRLEGYNVLFPIGWDAFGLPTENYAMQTGKHPRKVTD
                     DNIKIFTAQLNAAGYSFDWSRSVDTTDPEYYKWTQWIFVQLFKHGLAYKDKTYVNFCN
                     SCKVVLANEDSQNGICDRCGSQVVQMEKDVWFLKIRDYADKLLEGLDEVDYLPRIRIE
                     QENWIGRSYGAEVDFKLKDTEDNLRVFTTRPDTLYGVTFMVMAPEHPLIKKYENIIQN
                     LDEIKEYQEESKKKTEFERVQLAKGKTGVEIKGLTALNPVNGEEVPIWVADYVMMGYG
                     TGAIMGVPGHDQRDWEFAKKYNIPIIEVIGGGNIEEEAFTDVEDGILVNSGMINGLRV
                     KDAIEKITNYLVENNLGERKVNYKMKDWAFNRQRYWGEPIPIIHCPHCGMVPVPEEEL
                     PLLLPEVENYRPAETGESPLANIPEWVNVKCPVCDEDAKRETDTMPQWAGSSWYFLRY
                     IDPHNKEQIASKEKLEYWGPIDWYNGGMEHVTRHMIYSRFWHRFLYDIGVVPFKEPYA
                     KRTAQGLILGADGEKMSKSRGNVVNPNDIIDEYGADTLRTYIMFIGDYEKYAIWNDAS
                     VKGCKRFLDRVWKMQEFVNEEKGYSHKHLTLMHKTIKKVSEDFEAMKYNTAIAAMMSF
                     INEIYNDGSITRDELKTFITLLYPVAPHITEEIWQEQGFEGMLNQTEWPVWDEEKTVE
                     DVIELPVQVSGKVRGKILLPKDADVNTARKLAEADENIFKYIEGKTTIKEIYVPGKIY
                     NIVVK"
     gene            13868..14548
                     /locus_tag="PLB33_01375"
     CDS             13868..14548
                     /locus_tag="PLB33_01375"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOG62200.1"
                     /translation="MFDIKKYYNTYPYTGYMGYYAMMASCFCKKSYVRVLNTVNQPLD
                     IQVNDILMAENLKIGEFTKYVKFPPNSYHVLIHSPNGNLLFESDIDIDYNLAYTAVVS
                     ADEEDKKDICILMVPEQKAHNMTSNMSALRLANLCIDEPEVVMSASDGTILFSDIKYG
                     KVSCNVAIPSGRYTLQLKKPDGTEILDHWIDAAPYMHYTLFIVGMKNDGSIKIIVPED
                     GVNYLDLC"
     assembly_gap    14566..14665
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14776..15471
                     /locus_tag="PLB33_01380"
     CDS             14776..15471
                     /locus_tag="PLB33_01380"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOG62201.1"
                     /translation="MKIIGIIGGMGPLATVHLFERIVLRTKAEKDQDHIRILIDNNTK
                     IPDRTLAILGNEESPVNELVNSAKMLENSGADFLIMPCNTAHYFIEEIKKNISIPFFN
                     MPEEAVKHTYDKYAKDAVVGIMATDGTIKTKIYEKYYAELGIKTVVPIKTQEKIMEFI
                     YDVIKKGNYDAGTSLFFECADELKELGANVFLLGCTELSSAQYIYKLEGNFINPMEIL
                     AETSIIFAGGQLN"
     gene            15531..16352
                     /gene="purR"
                     /locus_tag="PLB33_01385"
     CDS             15531..16352
                     /gene="purR"
                     /locus_tag="PLB33_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HOG62202.1"
                     /translation="MKKYKRNQRIGALMKIFAEKPNCLFSYNYFCEIFNAAKSTISED
                     VAIVKELAAEMNFGRIETISGVSGGVMFIPILGKKELNEILEQLCSLFSQKNRILPGG
                     FIYMTDIFNNPRIVSNIAEVISSQFSNKHIDYIVTVETKGIPVAIMTGQKLNVPVAVI
                     RRNNKITEGATVSINYVSGSSNNIQTMSLSKRSLKENAEILIVDDFMKGGGTCKGMED
                     MMREFNANVVGTAVVIETIEPEKKLVDNYTSLLILNNKSIKEGVIEIKPNPKLLK"
     gene            16450..16743
                     /gene="spoVG"
                     /locus_tag="PLB33_01390"
     CDS             16450..16743
                     /gene="spoVG"
                     /locus_tag="PLB33_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOG62203.1"
                     /translation="MKISDVRVRKINSEGKMKAIVSVTFDDCFVVHDIKIIEGQEGLF
                     IAMPSRKMPDGEFKDIAHPINSETRNLVAEAVFKAYEEKRTEESENVEEAALM"
     gene            16954..18324
                     /gene="glmU"
                     /locus_tag="PLB33_01395"
     CDS             16954..18324
                     /gene="glmU"
                     /locus_tag="PLB33_01395"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOG62204.1"
                     /translation="MSMSIILAAGEGTRMKSDLPKTLHKVCGKEMIKYIIEASLNSGI
                     EKNIVVLGHGKEKVGHNIKDMNVQTVEQPIGEGHPYGTGYAVMAAENHIDDGETVLIL
                     CADGPLVKKETLIDFMNYHFENNCAASIMTAYLDNPRGYGRIVRKSDMSIDEIVEEKD
                     ASEEIKNIKEINTGIYCFKGELLKYALKKITKNNSQNEYYLTDTIKVLNNEGANIGGW
                     LLEDADDIKAVNDRVQLAEVTKIMQKRINQRHMYSGVTIIDPDSTYIDDSVKIGRDTV
                     IYPNTYLEGGTVIGSNCTIGPNSRIIDSTVRNNVTVESSKVIESYIDDGTNVGPFAYL
                     RPGSNLGKNVKIGDFVEVKKSIIGDNSKSSHLAYIGDAEVGKNVNIGCGVVFVNYDGK
                     KKHKTIVGDGAFVGSNSNLVAPVTIEKGGYVAAGSTITEDVKEGDLAIARARQVNKEG
                     RGKNRF"
     gene            18335..18904
                     /gene="pth"
                     /locus_tag="PLB33_01400"
     CDS             18335..18904
                     /gene="pth"
                     /locus_tag="PLB33_01400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOG62205.1"
                     /translation="MYVIAGLGNPGKEYASSRHNAGYMAVEYLAGKLNTKLNKLKFNS
                     VYGDTSINGEKVMLVKPVTYMNKSGIAIAEIVKFYKISTSNLIVIYDDIDIPLGALRI
                     RPSGSAGTHNGMKSIVDSIGSEFPRIRIGIGRNEEMDLADFVLQKFSRNEKDIVTPII
                     ERAAEAAVEIIENGIDSAMQKFNMKGDTP"
     gene            18962..22486
                     /gene="mfd"
                     /locus_tag="PLB33_01405"
     CDS             18962..22486
                     /gene="mfd"
                     /locus_tag="PLB33_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOG62206.1"
                     /translation="MNRILFDQFQRTEKFKKICSNYNKEKSQLIQGLNEEGLAYLACN
                     LLDTSDKILILTSSEAKSKKYEEIIRAYTEHSERLQPKEFILYNVDALSKDVEYKRAN
                     LLNNILNKRKCIVTASINSIITRVMSKDRFKESIIELKYGDSYDLNELRSNLIKLKYE
                     RVDAIEGVGQFSVRGGIIDIFSPSEANPFRVEFFDDEVDSIRLIDLKTQRSVKNIKSA
                     KILPCSDILFKKEEIKIILSEIEKDYYKQSQNQSLSKEAFKNLKNLYKSYQDKLMGGM
                     GIENADLLIPFIKESFSNIIDYFEDDSILLLDEPERIFEELNDLNESFQLKYTELFER
                     GEIFSNQSEVYISEHELLTKISKKPFISINGKEKIIKTDDSVHMLFKEAPSYYGKIEE
                     LSKDLNRLKYKGYKVDIILSTYESCSKLYNLLNDYECTTTLSKDTNAAESGQVVIAPG
                     ELTKGFEYYDNKLLVLTENEVLGSVKRKSRKTKKRKGAAIEIFTDLKVGDYVVHEHHG
                     IGQYIGIEKIEVQNIQKDYLCIKYKGEDKLYVPVDQMSLIQKYIGTDSEKPKLNKMGS
                     SDWIKTKERSKAAIENMASELIKLYAERKVLKGYAFSKDTEWQKEFEYKFPFEETEDQ
                     LRCVKEIKKDMEKPICMDRLLCGDVGFGKTEVAMRAAFKAVMDSKQVAMLVPTTILAQ
                     QHYTNLVERFRGYPIKIEMLSRFRTHYQQNKIINDLNKGLIDIVIGTHKLLSKELKFK
                     DLGLLIIDEEQRFGVRHKELIKQLKSNIDVLTLSATPIPRTLHMSMIGVRDMSIISEP
                     PGDRLPIQTYVIEYNEGLIKDAIEKEISRKGQVYYVHNRVADIDQTAAKLRKLLPEAR
                     IAVAHGQMSERHLENIMLEFVNKEYDILVCTTIIETGMDIPNVNTLIIDNADYLGLSQ
                     LYQLRGRIGRSNKVAFAYLTYEKDKMLSEVADKRLKAIKEFTEFGSGFKIAMRDLEIR
                     GSGNILGPEQHGHMLAIGYDLYVKFLDRAVKEIQGKQVEEDIETSVDLNVDGYIPSSF
                     IENEEQKIEIYKKIAAAENKDDIFDITEEIIDRFGNIPVQVDNLLKISYIKSLSKRIK
                     IKSIAQAGNTVNIEMTKDYLSQDIIEFLIKNYYEKISFVGTNEPIIKYKLDSLEQMKI
                     LRELEEFLETLNNFKAGKTAVEEKHEQI"
     gene            22473..23414
                     /locus_tag="PLB33_01410"
     CDS             22473..23414
                     /locus_tag="PLB33_01410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG62207.1"
                     /translation="MNKFKKSLLLITLLCIALVFTACSNSGGKDVDKEGVMATVNGKP
                     IMQKDYDDALAYYKDYVEYQLGEDAWETETPRGTYKEIYEEYVWDEMTKSLLLLDAAE
                     KEGIKASEEEVQHSLENFKINFENDEEYKSFMEQRGITEDFIIQELTKEILINNYITS
                     KIDSLNPNNEELKNLFEEMKLNVLIKASHILVDTEEEAVKIVERINKGEDFGDLAKEL
                     SIDTASGANGGDLDYFKYTDMVKPFSEAAFALEIGEVSQPVQSDFGYHIIKLTDKIVD
                     EETTVESEKEKLIQYYKLNKYEDLLEQLKNDAVIVKN"
     gene            23609..24160
                     /gene="spoVT"
                     /locus_tag="PLB33_01415"
     CDS             23609..24160
                     /gene="spoVT"
                     /locus_tag="PLB33_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOG62208.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEI
                     ILKKYSPIGELTEFAGEYVESLFETTRHIAIITDRDGVIAVSGSSKKDYAEKRLSPEL
                     EKIIESREMYQTSATSKPIRITANEFNPDNYTSQVIAPIMVHGDPIGAVILLSKDKGS
                     KMSEVEEKLIKTASIFLSRQMEN"
     gene            24533..>25825
                     /locus_tag="PLB33_01420"
     CDS             24533..>25825
                     /locus_tag="PLB33_01420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG62209.1"
                     /translation="MKIRFLGAVNGVTGSNHLIQIRGKNIMLDCGMYQGKDEELNAEE
                     FAINPADIDCLLLSHSHIDHSGRIPLLIKKGFKGAIYCSKPTYELCEIMLLDSAHIQE
                     AESQWKNNKAKRSGRKLVEPLYTQQDVLDSLQYFKPVLYDQIINIDEIITVKFNDAGH
                     ILGSSIIEIWFKDENDTMKLVYSGDLGMREKPILKDPAQIDKADYLIMEATYGNRVHE
                     NIEKRTEELINIILKTTKRGGSVIIPSFAVGRTQELIYELNKYYDSHLNEIGTKENEL
                     RKIPVYIDSPLAIKATEVFKRNSYAFDKEAKDYIMKGDNPLDFENLHFTVTADESKKL
                     NFSTEPKIIISASGMCDAGRIRHHLKHNLWRKEASIVFVGYQAEGTLGRRLLDGEKCV
                     KLFGEDIVVNAEIYNVEGFSGHADKPALLNWIGSFKEKP"
     assembly_gap    25826..25925
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26034..27809
                     /gene="iorA"
                     /locus_tag="PLB33_01425"
     CDS             26034..27809
                     /gene="iorA"
                     /locus_tag="PLB33_01425"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOG62210.1"
                     /translation="MKVLMTGNEAVAQGAYEAGIHFAAAYPGTPSTEILENIALRKEI
                     TAEWAPNEKVAVESAFGASLAGARTLTAMKHVGLNVAADPFFTIGYTGVNGGMVLVSA
                     DDPGLHSSQNEQDNRYYARMAKVAMLEPSNSQECKDMVIKAMEISEKYDVLVMLRMTT
                     RVCHSKSIVETGERIEVPVKPYVKNAPKYIMAPANAKAAHVVVEKKLKELEKFSNETE
                     LNYIEWNDKKIGVISAGVAYQYAKEVFGKDASYLKLGFTFPLPMEKIKKFAEEVETLY
                     VVEELEPFMEEQIKAAGIKCIGKEKIPNIWELNPDIVKKSIFGTENEIIDYDRTIAVG
                     RPPTLCAGCPHRAFYYQLSKKKNTIVTGDIGCYTLGGAPPLNSMDTCVCMGASISMAH
                     GAKKAFERNNDDKRVVAVIGDSTFFHSGMTSLLDVVFNKGNTITCILDNRITAMTGHQ
                     DNPGTGYTLQGDSTDVVQIEPIVRALGVKNIKTVNPMNQAEMKAALNWAYDLNEPSVI
                     ISRYPCALKKYTKEDLEEFGPIQGYCEVDEETCIGCKICVGTGCPAIVFKKDIKKSSI
                     NKFQCVGCELCLQLCPTKAISKVGE"
     gene            27811..>28343
                     /locus_tag="PLB33_01430"
     CDS             27811..>28343
                     /locus_tag="PLB33_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOG62211.1"
                     /translation="MKDTKNILLVGVGGQGTILVSKILSTGLMTAGYDVKMSEIHGMA
                     QRGGSVSTQVRYGSEVFSPIIGIGEADILVSFETMETLRWLEYLKPDGVVVINDFEIA
                     SAPILMGKAEYPEGIIELIKGKAKTKVINAGETAEKLGNQKVMNVVLLGALIKSMNLT
                     DIDWEAVIKSQVKEKFI"
     assembly_gap    28344..28443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28444..29344
                     /gene="buk"
                     /locus_tag="PLB33_01435"
     CDS             <28444..29344
                     /gene="buk"
                     /locus_tag="PLB33_01435"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOG62212.1"
                     /translation="SYLKEKQFDIKELSCVVARGGMLLPVKSGAYVINDIMLDRVENR
                     PVMEHASNLAAPIAYGIAKEAGVTSYIYDSVMTDEFPDISRISGMPDIPRKSASHVLN
                     TRAMAIKYAKSIGKSYEELNTIVAHLGGGISINIHHKGQIIDLVSDDEGPFSPERAGR
                     VPCRELINLCYSGKFDKKTMQRKLRGSGGLKAYLNTVDAREVESMIKEGNEYAKLIYE
                     AMAYQIAKGIGELATVVEGKVDVIILTGGIAHSEMLTSWIKNRVSFISHVEIMPGENE
                     MEALAFGALRVLRGEEKAREYIE"
     gene            29600..33325
                     /locus_tag="PLB33_01440"
     CDS             29600..33325
                     /locus_tag="PLB33_01440"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOG62213.1"
                     /translation="MNFDVKRVFVEKRAGFNTEALNLLNNLKENLNIEKLNNLRILNR
                     YTVSNIDEDDFKKSVNTVFSEPNQDIVYFEDVELEGHVFAVEYLPGQYDQRADFAAQC
                     IQIVTGKEKPTVKYARVFVLEGDLSKDDIDRIKKYYINPVDSREAQLDKPHTLDDEYP
                     MAEDVQTINLINKSKQELEEFRNDIGLAMSFEDLAFCQEYFKNIEKRNPTITEIKVID
                     TYWSDHCRHTTFSTIIENIEFEKGKITEEIKNTYDEYLNVRKYVYEGKKDITLMDLAT
                     IGAKELRKAGLLDDLEISEEINACSIIINADIDGKNEEWLLMFKNETHNHPTEIEPFG
                     GAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRQKIEDTLPGKLPQRKITTEAAAGYS
                     SYGNQIGLATGFVNEIYHSDYVAKRMEVGAVIAAAPRENVKRETPKKGDLVILLGGRT
                     GRDGIGGATGSSMEHTEESILLRGSEVQKGNAPTERKIQRLFRKKEVSTLIKKCNDFG
                     AGGVSVAIGELADGLNINLDLVPKKYDGLDGTELAISESQERMALVVRKEDADKFNEY
                     ANEENLESTVVAEVTDDRRLKMTWRGKTILDISRDFLDTNGVKQKTNARIKEPLNSKY
                     KRSFKSNNIREIWLENLNDLNVASQKGMIERFDSTVGAGSVLVPLGGKTQLTPAEGMA
                     AKIPVEEGITNTCSLMTYGFDPYLSKESPYHGAMYAVIHSIAKIVAMGGDYKNIRLSF
                     QEYYEKLNKEPERWGKPLSALLGAFKVQHELKIPSIGGKDSMSGTFNELNVPPTLISF
                     AVTVSDVKNIISPEFKRANNKVVLIKIKKDENNIPDFEDIGIKYSAIHEAIKTGKVLS
                     AHTVGYGGIAEAISKMTLGNEIGFRSDFTIGNDWFDSSYGDIVLEVDNSFLPFADCIE
                     IGKTINEKEIWVNGICISLEEIKTNWLKPLNNIFPVKKDGAGEIENYSFNSKHIAKSS
                     ISIAKPRVFIPVFPGTNCEYETKRAFERAGAKADIFVFKNLSSQDIKDSITEMNSLIR
                     QSQIIAFPGGFSAGDEPEGSGKFIAAVFRNELLKDAVTDLLNNRDGLIIGICNGFQAL
                     IKLGLVPFGEITDMENDYPTLTYNRIGRHIATFVKTKVVSNLSPWLGMCSVGDTHYLP
                     VSHGEGGFAANQKWIEKLKTNGQIATQYVDLNNNPTYDGFYNPNGSVEAIEGITSPDG
                     RVFGKMAHSERLDENLYKNIPGNKNQPIFESGVKYFK"
     gene            33336..33830
                     /gene="purE"
                     /locus_tag="PLB33_01445"
     CDS             33336..33830
                     /gene="purE"
                     /locus_tag="PLB33_01445"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOG62214.1"
                     /translation="MKVAIVMGSDSDFPVVEKGYKVLKDFEVMADVRVISAHRTPYEA
                     LEFARNAYKNGYDVIIAAAGKAAHLPGVLAGCTTVPVIGLPIKSSELDGMDALLSIVQ
                     MPPGVPVATVGINAAENAALLAIQILSVKYEDLRNKFLDYKKQMAEKVFEKDKKLNEK
                     LMSS"
     gene            33887..35275
                     /gene="purF"
                     /locus_tag="PLB33_01450"
     CDS             33887..35275
                     /gene="purF"
                     /locus_tag="PLB33_01450"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOG62215.1"
                     /translation="MFNLEEDKLHEECGVVGVYSDNPDITSQLIYYGLFALQHRGQES
                     AGIAIHTGKRIEFHKDMGLVADVLSQKVIDSLKGNIAIGHDRYSTSGESEKQNAQPLV
                     VRYKEGSIALAHNGNLVNADSIREILENEGVIFQTSTDSEVVANLISRHYRGSIIKSI
                     QTTMEVVKGAYAFVVMTDKELIGVRDNYGLRPLCLGKREDGYLLASESCGITAMGGEF
                     IRDIEPGEIVVINEEGVNSIKTTKYAQQRLCIFEHVYFARPDSNLDGVNVYETRYKSG
                     RLLAQEAPVKADLVFCVPDSGTPAALGYAEELSIPYGLGIIKNRYAKRTFIEPTDKLR
                     QQGVKTKLSILKEIVQDKVVVMVDDSIVRGTTTKQIVSMVREAGAKEVHVRIASPPVT
                     HSCFFGINTPDRKELLGANKTIEEIKDYIKADSVHFLSLENLIKSTGSNLGYCKACLN
                     GDYPMEVPNNGI"
     gene            35285..36325
                     /gene="purM"
                     /locus_tag="PLB33_01455"
     CDS             35285..36325
                     /gene="purM"
                     /locus_tag="PLB33_01455"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOG62216.1"
                     /translation="MTDKLTYKDSGVDVNSGYETVKLIKEHVRKTHIKGVISNIGGFG
                     GMFEIDKDEYEEPVLVSGTDGVGTKLMIAFMADKHDTIGEDAVAMCVNDVICQGAKPL
                     FFLDYIATGKLEPKKAASIVKGVSEGCIKAGCALIGGETAEMPGLYDVGEYDIAGFCV
                     GIVDKKKIIDGSKIIEGDILIGIPSSGIHSNGYSLVRKVFFSHKNYDITDYVEELGKT
                     LGEELLTPTRIYAKLVLDLISKFEIKGLSNITGGGFYENIPRMFPQGITADIDTKNIN
                     VLPIFKLLQKKGNIDIDEMYGTFNMGIGIVLTVAKDDADDILSYLKSVGETPVIIGRT
                     VKREGGLKLCHR"
     gene            36313..36939
                     /gene="purN"
                     /locus_tag="PLB33_01460"
     CDS             36313..36939
                     /gene="purN"
                     /locus_tag="PLB33_01460"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOG62217.1"
                     /translation="MPQVKIGVLISGSGTNLQSLIDNIETGNIKGKITVVISNRKDAY
                     GLNRARQKNIDALYIRQKDYESFENFNDAIIDELKKHNVELVVLAGYLKILTSKFIEE
                     YRNRIINIHPSLIPAFCGKGYYGIKVHEEVIKYGVKLSGATVHFVDEEADTGPIIIQE
                     AVEVGYEDTAETLQQKVLKIEHKILPLAVKYYCEGKIQISGRKVKIKE"
     gene            36941..38500
                     /gene="purH"
                     /locus_tag="PLB33_01465"
     CDS             36941..38500
                     /gene="purH"
                     /locus_tag="PLB33_01465"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOG62218.1"
                     /translation="MMRALISVSDKTGIVEFAKELERLGVEIISTGGTHKKLNEAGIK
                     VRGISEVTNFPECLDGRVKTLHPNIHAGLLAMRSNPEHMKQLLDLNIKPIDLVVVNLY
                     PFKQTVLKEGVSREEAIENIDIGGPTMLRSAAKNYQDVAVIVDPKDYELVLNELKENK
                     EVSLDTKFYLCSKVFSHTAHYDTLIASYIKRERKDNTLPDTYTMTFEKIQEMRYGENP
                     HQKAVFYKEVKNVDGLLASALQLHGKELSFNNINDTNGALELLKEFDEPCVVACKHAN
                     PCGVGCGDNIYEAYMKAFNADPTSIFGGIVVFNREIDERTAQEINKIFVEIIVAPSYS
                     QEALSVLMSKKNIRLLQIENILQKQDEYSYDIKKVAGGLLVQEIDSKLLPKGEELKTV
                     TSSKPTEKEMKDLFMAWKIVKHTKSNAIVIVKDGQSLGIGPGQVNRIWACKQAIEHAY
                     DFLGKDSTKGASLASDAFFPFPDCAEEAAKAGIRAIIQPGGSLKDDESIEVCNKYNIS
                     MVFTGMRHFKH"
     gene            38509..39762
                     /gene="purD"
                     /locus_tag="PLB33_01470"
     CDS             38509..39762
                     /gene="purD"
                     /locus_tag="PLB33_01470"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOG62219.1"
                     /translation="MKILVVGSGAREHTICWKLKQSSKLSKLYCAPGNAGISKIAECV
                     DIKADELEKLVEYSVKNSIDLVVVGPEVPLVAGLVDMLNEKGIKAFGPVKAGAKFEGS
                     KSYSKDFMKKYSIPSANYEIYTDSEKAISELNKFNTPIVVKADGLAAGKGVLICETHE
                     EAKEAIKSIMVKKLFGEAGNSVVIEEFLKGTEASLLCFVDGKNMIPMESARDYKKAYD
                     QDKGLNTGGMGCFSPNTIFTEELNKYIKENILDRTLNGFLNENIEFKGVLFIGLMIEN
                     NQAKVLEYNARFGDPETEVVLPRLKSDLIDIMLKCIEGNLTEDDLQWEDNKCVTVILA
                     SGGYPESYEKGKEITGINEVDDDIIIFHGGTKLSGNKTVTDGGRVLAVTAMGKSLEEA
                     RKKVYKNIPKINFEKMEYRTDIAKI"
BASE COUNT        14343 a         5761 c         8450 g        10914 t
ORIGIN      
        1 aagttattga catatgtcgg atatagtgta taatataaga taaatgacat atgtcgaaaa
       61 tgaaaggagg ataaagatgc cacgtagaga tggaacgggt cctataggaa tgggaccaat
      121 gacaggaaga ggttttggat tctgcggtca taggttttat ggcagaagaa attgctttgg
      181 aagaggtttt ggaccctggt atggtgcagg ttatggattt gcaccgggat atggacaggg
      241 atattttaaa caggatatga cagagaaaga atttctgaaa aaccaaaaag aattcttgga
      301 atcacaaata gagctacttg aaaaagaatt ggaagattta aaagacgatt agaaaggttg
      361 gagataatat gaaaatagca attccaacag aagaaaagtc aattaaatca aatatatatg
      421 attatcttgg tcgcgcaccg tatttcctta tctacagcac tgttacaaaa gaatacgaat
      481 ttttggacaa cagggcagta gttgaacagg gaggtgcagg tattagagct gcacaagtga
      541 ttgcggacaa tggtgtaaaa gctgtaatta caaatcggtg tggagagaat gcagaaaaat
      601 tattcagaaa tgctgaggtc tttatttatg cagcccaaaa aggcactgca gaggaaaata
      661 tagcatattt taatgaaaat aaactttctt tattaaatga atttcactca agctttcacg
      721 gcaaagaaaa ataaatattg acatatgccg attataaata tataatttat tattggcata
      781 tgtcataaat atggaggaaa ttatgagaat agcaatcccg gtagatgatg gaagtgtatg
      841 tgtatcattt ggcaggacgc catattttat gctttatgat acagaaaata acgaaagtag
      901 ttttattaat aacagtgcgg caaacagtca aggcggtgca ggtattaaag cagctcagat
      961 tattgtagat aataatgcag atatattaat aactccaaga tgcgggcaaa atgcagcaga
     1021 ggtgttaaca gcagctaatg ttaaaattta taaagcatta aatgattcaa ttgaagaaaa
     1081 tttaaccgca tttaaaaatg ataaactaaa aatactgact gaaatacatt caggttttca
     1141 tggtggagag aaataatgat gattgctgtt ttaagcggca agggcggaac aggcaaaaca
     1201 tttgtatctg taaatttatc ttttgttgcc gataattcaa attacattga ttgtgatgtg
     1261 gaagaaccta acggacatct tttctttaag cctaatatta ttaaaagtga aaaggtgcac
     1321 gttaaaattc caaacataaa taatgagctt tgcaacgggt gcagaagatg cgtagatttt
     1381 tgcaagttca atgctttggc atatataaaa gacaaagtat atgtatttga tgagatatgt
     1441 cattcttgcg gaggatgcat gctgttatgc cccgaaaaag caatatcgga aaaagataaa
     1501 gaaataggag aaatacagga gggcattttt aaaaattcga atatttatac cggaatttta
     1561 aatcccggtg aagaaaccgg agtaagtata atcaagaaac tgttaaaaat cgccggcaat
     1621 aaaccatata cctttatcga ttgtcctccg ggtagctcct gcagcgttat ggaaagcata
     1681 aaggatgttg attactgcat attggtggct gagcccacct tatttggcgt acataatttc
     1741 aacatggttt ataatttagt taaattattt gataaacctt tcggtgtagt attaaataaa
     1801 tgcctagacg gagaaaatcc ggcggaaaaa tattgtaatg aaaaaaatat taaagtttta
     1861 tgcaaaatac ctttcgagca tgaattaggc aatataaatt cgaatgcaga aattgcatct
     1921 gaaaaaaatg aaaaatataa aagtctattc tcctctcttt taaaaacaat aaaggaggag
     1981 gttaaatgaa gcagctgtta attctaagcg gtaaaggcgg cacagggaag acaaccgttg
     2041 ccggtgcaat tatcaaactt tcaaatgtaa aagcttacgc tgactgcgac atagatgctc
     2101 ctaatctgca tttgattatg caacaagaat ccataccgga gaattcggat tattacggat
     2161 tagataaagc agtgattgat aaggataaat gcctaatgtg taatttgtgt tatgatagct
     2221 gccgatttga tgcaatcagt gattacactg tcagtctcta tgattgtgaa ggatgcggag
     2281 tctgtgaagc tgtttgtcct gcttcagcca taaatttgat gccttataaa gcaggagata
     2341 tgacattata ccgaggcaat caagtttttt caaccgccga attaaagatg ggaagcggga
     2401 attcaggcat gcttgtaaca gaagtaaaga aaaacatgag gtttgcagca gcggatgctg
     2461 attttgcaat aattgacggc tcgccgggca tagggtgtcc tgttattgca tcaataagcg
     2521 gtgtcactat ggtactggtt gttgccgagc cttctttgtc gggaatcagc gatatgaaaa
     2581 gaattataga aacagcaaaa aaatttaata caaaattagc cgtttgcgta aataaatatg
     2641 ataccaatat tgcaaacaat gaaaagataa aacgattttg tcaagataat aaaattgatt
     2701 ttgtggggga aattcctttc gataaaaaag ccgtggaagc tgtaaataat ggattaacaa
     2761 ttgttgatat tgattgtgcg gcaggtgaag ccgtgcgaaa aatatataaa aagacaatga
     2821 aaattttatt caattaggag aagaaaatga gtgaaaattg taatgaaagc tgtagttcct
     2881 gcagtaagga atgcgacagc agaaaagaag aacaaactaa ttttttggaa aggccgcacc
     2941 ctcaaagcaa catcaaaaaa gtgattggtg ttataagcgg taagggtgga gtaggcaaat
     3001 cattggtaac atccttgctg gcagttacca tgaagagaag aaatcataat gttgcaatat
     3061 tggatgctga tattacaggg ccttcaatac caaaattatt tggtataacc gaaaaagcaa
     3121 ggggtggttc caatggggta ctgcctgtga aaagtaagac cggcatagaa gtaatgtcaa
     3181 ttaatcttct gttggaaaac actaccgacc ctgttgtatg gagaggcccc ataatagcag
     3241 gaacggtaaa acagttttgg acagatgtaa tttggaaaga catagatttt atgttcattg
     3301 acatgccccc gggaacaggt gatgtgccac ttactgtatt tcaatccata ccggttgatg
     3361 gaatcattgt attaacatct cctcaggaat tggtgtcaat gattgtggag aaagcggtga
     3421 aaatggctga atcgatgaat attccaatca ttggaatagt tgaaaatatg tcatatttca
     3481 aatgtcctga ctgcaataaa gaatacaaaa tattcggcga cagtaaaatt gaagatgtag
     3541 caaagaaaca cggtttgaat gttattgcca aactacccat agatccaaaa atatcatcca
     3601 tgtgcgacaa gggattgatt gagctttatg gagatgaacc atttaccggt atagctgaat
     3661 ttttagaaga gatggagggt tagaatgaaa atagcagttg caagtgaaaa ggaaatagtt
     3721 acggagcatt tcggtcattg tgagaatttt aatatttatg aaacagatgg aaataaaata
     3781 gtaaaaagcg agtcagttaa aaatccgggt cacagaccgg gatttctacc aaacttttta
     3841 aatgatatag gtgttaatgt aataatatca ggaggaatgg gccagggagc aattgatata
     3901 tttaatgaaa aaggtatcga agtaataacg ggtgctctag gtgaagcaaa aattgccgca
     3961 gaagaatatt taagaggtaa tttaaaatca acgggctata tttgtcatga gcaccagcat
     4021 catggtgaat gtgaataatt aatgaaaagc aattttaaag ttataaagac gatttcttgg
     4081 ttaaacaatc aatgaatcgt ctttttgtat tatatctata gtatgatacc ttttagaaaa
     4141 cccttggaag caaaaatgca aaggcgcaga ggttggttat tacaatatta cttgattatc
     4201 aaaagatgta aaataatatt tctgttaaaa ttctcataga tgaaaatact atatagacaa
     4261 agaagaacta acaatagata taaaacaatt ttcaaagaaa acagagcata aatacagata
     4321 aggtatgttt tttatgtatt aggagcgatg gttttggatt cggatagagt atggaaaata
     4381 gttatattaa ttatatgttt agtattatcc gccttatttt cggcatcaga gacggctttg
     4441 atgtccttaa gcaaaattcg tgttaaacaa atgattgaaa atagggaaaa aggtgcaaat
     4501 aggataaata aattgcttag tgacccaagc aggctgttaa gcgcaatact tatcggtaac
     4561 aacgtggtta atatcggtgc atcatcatta atgacatcat tagctatcga tgcattcggc
     4621 aataccggcg taggtgtcgc aaccggaata atgaccttat taatcctggt atttggagaa
     4681 ataactccga aatcattagc agctaaaaat tcagaaaaga tatcggtaag actatccggt
     4741 tttatagaat ttgttacaaa tcttttaact ccaatcagtt ttgtgttaaa tattataact
     4801 gattttttgg ttaaattatt gggtggcgaa gttgataaaa aaaagccctt tattacacaa
     4861 gaagaattaa aaactatagt aaatgtaagt tacaaagaag gagtattaga aggtgaagaa
     4921 aaggatatga tttacaatgt gtttgatttt agtgattcac aagttaatga tgtgatggtt
     4981 ccaaggacag aaattgttgc cattgacgtt gatttgcctt atgaagaaat tataaaaata
     5041 ataaataaag agcaatattc cagaattccc gtatatgaaa acacaattga taatataatt
     5101 ggaattttgt atgtaaaaga tttgttattt ttggatgtta ataaagaatc tccctttgat
     5161 ttaaggaaat atatacgcca accatatttc actcccgaat acaaatccat taaagagctg
     5221 tttaaggaaa tgagaacaaa ccgaaatcat atggttgtta ttatagatga atatggcgga
     5281 acagaaggaa ttgtgacaat cgaggatgta gtagaagaaa tagtcggtga tatagaagat
     5341 gaatatgata aaaaaattaa agaaatcgaa gtaataaaag aagatgaata tcttgttaac
     5401 ggtaatgtaa gaattgatac aataaacgaa ttaatcggaa ctcatataga gtctaaggac
     5461 tttgatacaa tagcaggttt tgtaatcggt attattgaca ggcttcctga agcaggagaa
     5521 gaaattgagt atgaaaatat aaggtttata atagaaaaca ttgatagaaa caggataaaa
     5581 aaaataagga tattaacata gactgtggag ggtataaatt agagcatctt aattgatgct
     5641 ttttttatga aatttttaat aatcaccctt agggtgattg acattatatg gaaaaaacgc
     5701 aataattatt taacacaatt aataaggggg aaaaagtatg aaaaaaggga cgggattttt
     5761 tcacagaagc atttcactga tagttttgtt tggggtacta atatgcttac ctgcttctgc
     5821 cttatccttt ataccaaaat tcgaaaccga gtctataaat tttgatggca ctcagagttc
     5881 ctgggcagag tcagagctta aagaagcata tgaaaacggt cttacttacg gtcaaataga
     5941 aaataattat caaaaaccta ttacccgtga ggaattttgt gttattgttg tgaagctata
     6001 tgaaaagtta ggggggaaag cccccgtaat gggaaccaat ccttttacgg acactgataa
     6061 tgcggaaatc ttaaaagcct atgccctggg tattgtaaaa ggaacatctg atactacttt
     6121 tacacctgca aacaatatta ccaggcagga aatttgtgta atgatattaa gggcattaaa
     6181 ggctgccaaa ccgggtatcg acacaagtct tggcagcgat tttccattcc aggattcgtc
     6241 aagcatagct tcctgggccc ttgatgcaat gaaatttgcc tacaagaatg atattatgaa
     6301 gggaaccagc gcaactacca tatcacctct gaataatacc aaccgagagc aggccattgt
     6361 tttgttgaaa cgtaccttta ccaagtattc ggaagcacct gaagcccagg gccaaggcga
     6421 tcccaatgaa tattattcaa gtttattaaa cggcagagct ccatcattga ataagaatgt
     6481 attcaagcct tttgttccgg ccaatgactc aggttcctca tcctattcgc agttcaccgc
     6541 aggtactgcc ttgtccggcg gtattgttat cagtcctaca ggaccgggta tggatattat
     6601 tgactcacta aaagaaaaac cgggttcaga tctgcgactc ttaacaccgg aagaaattga
     6661 gaaatactgg gatgaatact atgactttcc cgacgaaagt gattatgaac catcagttga
     6721 ttgggttaaa gcaagtgaca atgtattggg agtagactgt ttaggcaagg gatatgatgt
     6781 aataaaggga aaattcgctg acccatacag tttaaaagac tctgtactta gtactaataa
     6841 gcttttagaa gctaagagag tgtttaaaac tgacggaaga cagagtgtca gccgctttac
     6901 cgaaggtaaa agtttatatt catattctaa ggaaatggcc agaaaaatag gcgtaagcgg
     6961 aggttatctg tatttcagtg gaagtatcaa tgctagtttc ggctctcaga cttacacaga
     7021 aactaacagt agatatgcaa ccttagtcag tgatttatct cagtatctga ttgaatttga
     7081 tgacaactat ttggactact ctaaatacta tgactcttca ttcaaaaagg atatagaaga
     7141 tgatagtgtt acgccggaag aaatctttaa ccgatatggc actcatgtga taagggctgt
     7201 taaaatggga ggtcgtttag actataatgt tcaagccaac agttcctatc acagcgggag
     7261 tcatacattt gaatcagaag taaaagcttc ttttaatatg gcctttgctt cagccggagc
     7321 cagtcattca acaagccaga aagaaaccac cacttctttt aacgagaatt gtgagattaa
     7381 tattaaagct tatcctgatt acctgggctc aaatactatt actccttcac aatatacggc
     7441 atggggagaa tatgtgcgaa aatacccggc aatgtgtgat tttggagaag acacaccctt
     7501 aataccaatt tataatttgg ctagaaatcc ttttagaagg tatgaactta agaaggctta
     7561 tgaaaaatat gcggaagaaa atcaatatat tcccacggcc gccataacct gtataaatgg
     7621 tattcgcctg aatccccagc ctgtcggcag aactttagac ggtttggacg aacaagttat
     7681 tattgacccc ataacaaatg ataaatggta tttagtagct aatatcaccg ctcacgattt
     7741 ttatcgcgat gaaatacaac aacaaatata tattagaaca ggtttcagcg atttggcttc
     7801 aaggccgcct gttgttgcag tattcttggt aaatgaaagc cagggtgaaa atgccaagga
     7861 aatatttaaa aaatattggg gcaatgaccc aacggccaag ctttggggtg atgggggaga
     7921 agcaaaatcc gaccccagtc tgaagaatga aatcagttcc tacgcagaca gattaaagct
     7981 ttattatgtg acaagcacca agggaagacc tattactgct cttagggtta aacactttgg
     8041 atatgacaat ctcatacgct attatcctcc aaaggatact aacagtcccc acgacgacta
     8101 tggtaagttc ttctctgtaa ttgattatgg gacccatatg aaacaaggta taaacgagaa
     8161 tcagaattgt gcggagggag cagttcccct accatttggt cctgcacacc agaatattta
     8221 tctccagtat tctcacgact actcaatcag ttattaattg catagacata agcttattaa
     8281 gacgccttct ttgtataaga aggcgttaag tttataaaca agtatacaac acattgtctt
     8341 gttattaata cacgtaagtg tcattctgaa cgcagtgaag aatctcatga gatccttcgc
     8401 atatgctcag gatgacattt ttttattatt tcactgttcc atatggccag gaatttattc
     8461 cgccaaaatc atagacattt gtataaccca ttttgattaa ttgtttggct gctgtggcac
     8521 ttctgtttcc ggtcctgcaa tatacaagta tttttgcatc cttgtcagcc agtatttctc
     8581 ctgctttacc tgttatatca tttaccggca gcaagacaga attctcaatg tgaccttcat
     8641 tgtattcttc ctgtgttcga acatctaaaa taataacatc ttcactgtca attattgctt
     8701 ttgcttcttc agcagaaatt tttttgtaag cagtctctgt acctgtttta tttttttcca
     8761 ttgatgtaca tcctgtaatt gttatagtta atgtaattat tataaatagt ataatcaaaa
     8821 gccttttcat aattaatcct tcccgaattt aatactttat atttactcca ccactatctc
     8881 aagcaaccac ttgacccata cggagccgtc ataactgggg atgactactc gtaacggaaa
     8941 gccttgcagc accggaagtg tcttaccctc taattcgtag gcaaggaaat tctcgggggc
     9001 gagggcattc tccagaatca tagaaatttc gtagccgtca gcgcccttaa aggcaaccct
     9061 ggtgctcccg ggctgcactt ttgccatctc gaggattgtc ttgagcgatg cgcccgacca
     9121 ggtagcaaca tccttgaaat atccaacgca ttccaatgtc ggagtagcag tcagctttgg
     9181 catgcgccgc aaatcatcat aggtcaggct cagctcttgg tcgaccttac cgctcacctt
     9241 gagccgatag gtgtcgaaat caaccaatgt cggtgtccca gtcattgtta gaccggtctc
     9301 cggatcagca ccgaggtagg gtggaataac ttcaggcata gtcggcacaa cgatgggctc
     9361 caactcgtcg ggcgggtcgg ttatcgttac cacaatggac tctccggctg gtgtgttcgg
     9421 gcttgtcgtg tcggtttctc ctatggcgca gccggatgcc attgttccaa gcaggatgac
     9481 cagcatcatg ataatcgtca atctatgtct gtgcagcata atcttccgct taagttcatg
     9541 cagatactcg atgtgtttat tataagttat catagttttg tactccttgt tatttttggt
     9601 agtgttaagc agacactcct ctatatagtc acactatcat ttaattaaat aaatgttacc
     9661 cgttagtaat aattttaaac aattataaga atgttagaat ttaatatgaa attaagtaac
     9721 acataagaag agaaatatta tataatagga gcatataaat ttaaaactat aaagggggaa
     9781 gcatggataa acaaacaatt gcaagattag cagcagaatt tgtgcaaaaa gagataaatt
     9841 atgtatcaaa tgacaaggca ttaaatgaag agttggcagg tttgaaaatt tttgatgaac
     9901 caatatttgc ttatggagct gcagacgatg aaggttttca attattaaaa tcaccttcag
     9961 ttatcggaga acattttatg atgccgaagg aatggcttca aagtgcaaat acagttatag
    10021 tttatttctt tccgtttacg gatgaaatca gagaaggtaa cagaacagat atgagctggc
    10081 cttcaaatga atggcttcac ggacgcattg atggacaaat ttttatgaat gaattcaact
    10141 tatttctaaa ttccaaactg aatgaagaag gctataaaag cttggttccc ggattggatt
    10201 caaggttcaa gtcagagggt ttctccagca attggtcaga aaggcatgta gcttttattt
    10261 gcgggcttgg gacttttgga ttatcaaaag ggataattac tcaaaaagga atggccggaa
    10321 gattaggaag tatagtgacg gaattaaaat taaaatcgga tataaaaaaa tataataata
    10381 tatatgaata ctgcataaat tgcggtatat gtgcagaaaa ttgtcctgca ggtgcaatat
    10441 ccttagaaaa tggaaaagac cataaaaagt gttccgactt tttggataag gttaaggaaa
    10501 agcataaacc ttattatggc tgcggcaaat gccaggtatg tgttccctgt gaaaatgaga
    10561 atccaaagga ttgatattct gatttattga catatttcat aaatcagata taatataagc
    10621 tataataaga aatggggtgt tattaatgcc tagaccgaga aaatggagaa gggtctgcag
    10681 cctgcctgat gtaaatcgct ttggaccttt ggattttgcg gtagaaaagg aacatgttgt
    10741 tattatgtcc gttgatgaat atgaaactat aagacttata gatttagaag gcctttccca
    10801 agaggaatgt gcaagtttaa tgcatgtggc aagaactacc gtccagggta tttaccagga
    10861 agccagaaag aaaattgcag aatccctggt aaacgggaaa gttttgcgaa tagaaggcgg
    10921 agattataag ctttgcgacg gcgaaggaga ttttcacagc acaagatgct gtcacagaaa
    10981 cagatgcggc aaagctaaat tgtaaagatt tacaattttg ttattgcaat acgttttttg
    11041 ttctgatata atcgaatgaa aagttaataa aatacaatga atggaagtag taggctgtaa
    11101 acaatcaaag agagccgtcg ggtggtgtga gacggtattg ctaatgctga actcgtccgg
    11161 gagtatttga agcgaaaaaa gtagtttcaa acggtgatac cgttataata ttgagtgagg
    11221 cttaggccta atttgggtgg taccgcggaa tcttcgtccc aatcgggagg aagattttta
    11281 ttgtcaacaa attggttatg gagctgcgat ttagtaaaaa catggaggcg aaaaatgaaa
    11341 tcatataatc cacaagctat tgaaaaaaaa tggcaggaaa tatggagaaa gaatgaaact
    11401 tttaaagcga aagacaatac agaccagaaa aagttttatg gtcttgtgga atttccatat
    11461 ccttcagggg taggtctgca cgtagggcac attcgtgcct atacatccct tgaagtaata
    11521 tcaagaaaaa ggagattaga aggatacaat gtattgtttc ccataggatg ggatgctttt
    11581 ggtcttccta cggaaaatta tgcaatgcag acaggaaaac atccaagaaa ggttacggat
    11641 gataatatca agattttcac tgcacagctt aatgctgcgg gatattcatt tgactggtcc
    11701 agaagcgttg atacgacaga cccggagtat tataaatgga ctcaatggat atttgttcaa
    11761 ttatttaagc atgggttggc ttataaggat aaaacatatg taaacttctg caacagctgt
    11821 aaggttgtac ttgcaaatga agattcgcaa aacggcatat gcgacagatg cggcagccag
    11881 gtggtgcaga tggaaaaaga tgtatggttt ctaaaaatca gagattatgc ggataagctt
    11941 ttagagggtc tggatgaagt tgattatttg ccgcgtataa gaattgagca ggaaaactgg
    12001 ataggtcgtt cttatggtgc cgaagttgat tttaaattaa aggacacaga ggacaattta
    12061 agagtattta ctacaagacc ggataccttg tacggagtta catttatggt aatggctccc
    12121 gaacatcctt tgattaaaaa gtatgaaaat ataatccaaa atctggatga aataaaagaa
    12181 taccaggaag agtccaaaaa gaaaaccgaa tttgaaaggg tgcagttagc aaaaggaaag
    12241 acaggagtag aaataaaagg actgactgct cttaatcccg ttaatgggga agaagtgcct
    12301 atttgggttg ccgactatgt tatgatgggt tatggaacag gtgctattat gggtgttccc
    12361 ggtcatgacc aaagagactg ggagtttgca aaaaaataca atattcccat aattgaagta
    12421 atcggcggag gaaatatcga agaagaagca tttacggatg tagaagacgg aatattggta
    12481 aattccggta tgattaacgg tttgagagtt aaggatgcaa ttgaaaaaat tacaaattat
    12541 ttggttgaaa ataatttagg agaaagaaaa gtaaattaca agatgaagga ttgggccttt
    12601 aacaggcaga gatactgggg tgagccgatt cctattattc attgtcctca ttgcggtatg
    12661 gttcccgtgc cggaagaaga gcttcccttg ttactgcctg aagtcgaaaa ttatcgtcct
    12721 gccgaaacgg gagaatcacc gcttgccaat atacctgaat gggtaaatgt aaaatgtccc
    12781 gtatgcgatg aagatgcaaa aagagaaact gacactatgc ctcaatgggc agggtcatca
    12841 tggtattttt taagatatat tgacccccat aataaagagc aaatagcatc aaaagaaaag
    12901 ttagaatact ggggtcccat cgactggtac aacggaggga tggagcacgt tacaagacac
    12961 atgatttatt caaggttctg gcacaggttc ctctatgaca taggagttgt accctttaag
    13021 gagccatatg ctaaacgtac cgctcaaggc ttaatccttg gtgcagacgg agaaaaaatg
    13081 tcaaagtcga gaggaaatgt ggtaaatcca aacgatataa tagatgagta cggtgcagat
    13141 actttaagaa catacattat gtttatcgga gactatgaaa agtatgctat ttggaatgat
    13201 gcaagtgtaa aaggatgtaa aagattttta gacagagtct ggaaaatgca ggagtttgta
    13261 aatgaagaaa agggatattc tcataaacat ttaactctta tgcataaaac aataaaaaag
    13321 gtcagcgaag atttcgaagc tatgaaatat aatactgcaa tagctgccat gatgagcttt
    13381 attaatgaaa tttacaacga tggaagtatt acaagggatg aattaaaaac attcattaca
    13441 ctgctttatc ccgtggctcc ccatataact gaagaaatat ggcaggaaca aggttttgaa
    13501 ggcatgttaa atcagacaga atggcctgta tgggatgaag aaaaaacagt tgaggatgtt
    13561 atagagcttc ctgtccaggt aagcggaaag gtaagaggga aaattttatt gccgaaggat
    13621 gcggatgtaa atactgcaag gaaattggct gaagctgatg agaatatttt taaatatata
    13681 gaaggcaaaa caaccataaa agaaatatat gtaccgggga aaatatacaa tatagtagta
    13741 aaataaatat taggaaatct ctcgacaagg tcgggagatt ttttttatgt tattgtcagt
    13801 atttgtcaaa catcttttta taactattaa caggggaaat tcatatattg gagagagggg
    13861 tgaatttatg tttgatataa aaaagtatta taatacatat ccatatacag gatatatggg
    13921 ttattatgca atgatggcaa gttgtttttg caaaaaatct tatgtaagag tgttaaatac
    13981 tgttaatcag cctttggata tacaggtaaa tgacatactt atggcagaga atttaaaaat
    14041 cggagaattc acaaaatatg ttaagtttcc tccgaattct taccatgttt taatacactc
    14101 tccaaatggt aatttgttat ttgaatcgga tatagacata gattataact tggcatacac
    14161 agcagtcgtc tcagctgatg aagaagataa gaaggatatt tgcattttaa tggtacccga
    14221 gcaaaaagca cataatatga caagtaatat gtcagcattg cggttggcaa atttgtgtat
    14281 cgatgaacca gaagtagtaa tgtcggcgtc ggacggaact attctatttt ctgatattaa
    14341 gtatggaaaa gtatcgtgta atgtggcaat cccatcggga agatatacat tgcaattaaa
    14401 gaaacccgac ggcactgaaa tcttagatca ttggatagat gctgcacctt acatgcatta
    14461 taccttgttt attgtaggga tgaaaaatga tggcagcata aaaattattg tacctgaaga
    14521 cggagtaaat tatttggatt tatgttaatt aatgacaagg ggacgnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnntaatg acaaggggac gggggtgttg tcatctttga
    14701 tgacaacacc cccgtcccct tgtcacaatg caagtaatat gatataatct agcaataaaa
    14761 attatgaggt ggactatgaa aataatagga attataggcg gtatggggcc tcttgccaca
    14821 gtacacttat ttgaaagaat tgttttaaga acaaaggcag aaaaggacca agatcacatt
    14881 cgtatcttaa ttgataacaa tacaaaaatt ccggacagaa ctttagccat actcggtaat
    14941 gaagaaagtc cggtaaatga attggttaat tcagcgaaga tgcttgaaaa cagcggtgcg
    15001 gactttttaa taatgccctg caatactgca cattacttta tagaggaaat aaagaaaaat
    15061 atcagcattc ccttttttaa catgcctgaa gaggcagtta agcatacata tgacaaatat
    15121 gcaaaggatg ctgtagttgg aataatggct actgatggaa ctattaaaac aaaaatttat
    15181 gaaaaatatt atgccgagtt ggggataaaa acagttgtac cgataaaaac acaggaaaaa
    15241 ataatggaat tcatttatga tgttataaaa aaaggcaatt acgatgcagg tacaagtctt
    15301 ttctttgaat gcgcagatga attaaaggaa ttgggagcaa acgtattttt gttaggctgc
    15361 actgagcttt cgtcagcaca atatatatat aaattggaag gaaactttat taaccccatg
    15421 gagattttgg ctgaaacctc aataatattt gcaggagggc agctgaatta agtcttgatt
    15481 tatacgaaca tttgtaatat aattatataa aaacattcgc tggtgataaa atgaaaaaat
    15541 acaaaagaaa tcaaagaatc ggtgcattga tgaaaatttt tgccgaaaaa ccaaattgtt
    15601 tgttttcata taattatttt tgtgaaattt tcaatgcagc aaaatcaaca ataagtgaag
    15661 atgtggcaat agtcaaggaa ttggctgcag agatgaattt tggcaggatt gaaaccattt
    15721 caggagtttc aggcggagtt atgtttatac ccatattggg taagaaggaa ttaaatgaaa
    15781 tacttgagca gttatgctca ttattttcgc aaaaaaaccg aatactgccc ggcggtttta
    15841 tttatatgac cgatattttc aacaaccctc gaattgtgtc gaatattgcc gaagtaattt
    15901 cttcacaatt tagtaataaa catattgatt atatagttac cgtagagacg aaaggtatac
    15961 ccgtagctat tatgacaggc caaaagctga atgttcctgt ggccgtaatc aggagaaaca
    16021 acaaaattac agaaggggca acagtcagca taaactatgt ttcgggttca tcaaacaata
    16081 ttcaaaccat gtcattatca aagaggtctt taaaagaaaa tgctgaaatt ctaatagttg
    16141 acgactttat gaaaggcggc ggaacctgca aaggaatgga ggatatgatg agggaattta
    16201 atgcaaatgt tgtagggact gctgttgtta ttgaaactat agagccggaa aagaaattag
    16261 tagataatta tacttcatta ttgatactta ataataaaag tataaaagaa ggtgttatcg
    16321 aaataaaacc aaatccgaaa ttgttaaaat aatttcatat atttatgaaa atgttttttt
    16381 gctatttttg ggtatttata gtataatgaa taaaattgga cataattatt gggggaaggt
    16441 ggtgacagta tgaaaatatc agacgtaagg gtaagaaaaa ttaattctga gggcaagatg
    16501 aaggcaatcg tttccgtaac ctttgatgat tgtttcgtgg tacatgatat taaaataatt
    16561 gaaggtcaag aagggttgtt tatagcaatg cccagtagga agatgccgga cggagagttt
    16621 aaagatatag ctcatccaat caattctgaa acaaggaatc ttgttgcaga ggctgtattt
    16681 aaagcatatg aagaaaagcg cacagaagaa agcgaaaacg tggaagaagc cgctcttatg
    16741 tagcgctgtt actgatgatt tacagtcaac agtactcggg tctttcatag acccttttat
    16801 tgtgtctaaa ggtaatatga atcaaaattg gtgaatttcc tctatcaatt ttttttaaat
    16861 attagagtaa tgtccctgaa tattttttga cttactaaat attatgatat ataataactc
    16921 agtattacag attcgcatta aggagaattt attatgagta tgtcaataat tttggcagca
    16981 ggcgaaggta ccagaatgaa atcagatcta ccgaaaacgc tgcataaagt atgtggaaaa
    17041 gaaatgataa aatacataat tgaagcatct ttaaattcag gtatagagaa aaatattgtt
    17101 gttttgggac atggaaaaga aaaagttgga cacaatatta aggatatgaa tgtacaaact
    17161 gttgaacagc ctatcggtga agggcatccc tatggaacgg gctatgccgt tatggctgca
    17221 gaaaaccaca tcgatgacgg ggaaacggtt ttaatacttt gtgctgacgg accgcttgtt
    17281 aaaaaagaaa cattaattga ctttatgaac tatcattttg agaataactg tgctgcatcg
    17341 ataatgacag cttatttaga taatccaagg ggatacggaa gaatagtaag aaagtcggat
    17401 atgtctatcg atgaaatagt agaagaaaag gatgcatctg aagaaattaa aaatataaag
    17461 gaaataaata cggggatata ctgttttaag ggtgagctct taaaatatgc tttgaagaaa
    17521 attaccaaga ataactcaca gaatgagtat tatcttacgg ataccataaa ggttttaaat
    17581 aatgaaggtg ctaatatagg agggtggcta ttagaggacg cagatgatat caaggctgta
    17641 aatgacaggg tacaacttgc tgaagtaaca aaaataatgc aaaaaagaat aaatcaaaga
    17701 catatgtatt caggtgttac aatcattgac ccggatagca catatataga tgattcggta
    17761 aaaataggca gagatactgt tatttatcct aatacatatt tggagggagg aacggttata
    17821 ggaagcaact gcacaatcgg accaaactct aggataattg attccaccgt aagaaataat
    17881 gtaaccgttg aaagctcaaa agttattgaa tcttatatcg atgacggaac aaatgtagga
    17941 ccctttgcat atttacgacc cggcagcaat ttaggtaaaa atgtaaaaat tggtgatttt
    18001 gttgaagtaa agaaatcaat cattggcgac aattcaaaat catctcattt ggcttacata
    18061 ggtgatgcag aggtaggtaa aaatgttaat ataggatgcg gagttgtatt tgttaattat
    18121 gatggaaaga agaagcataa aacaatagtc ggcgacggtg ctttcgtagg aagcaattca
    18181 aatttagttg ctcccgttac tattgaaaaa ggcggatatg tggctgcagg ctctaccatt
    18241 acggaagatg tcaaagaggg agatttagca attgcaagag ctaggcaggt caataaagaa
    18301 ggaagaggca aaaacagatt ttaaagaggt taatatgtat gtaattgcag gacttggaaa
    18361 tccaggtaag gaatatgcaa gctcaagaca taatgcaggt tatatggcag tagaatattt
    18421 agctggaaaa cttaatacta aattgaataa actaaaattt aactccgtat atggggatac
    18481 ttccataaac ggagaaaaag tcatgcttgt taaacccgta acctacatga ataaaagcgg
    18541 aattgccata gcagaaatag ttaaatttta taaaatttca acgtcaaatc tcatagttat
    18601 ttacgatgat atagatatac ccctgggggc tttaagaata agaccgagtg gaagtgccgg
    18661 cactcataat gggatgaaat caatagttga cagtatcggg agcgagtttc cgcgcataag
    18721 aataggaata ggaagaaatg aggaaatgga tttggcagat tttgtgctgc agaaattttc
    18781 aaggaatgaa aaagatatag tcactcccat tattgaaagg gcagctgaag cagcggtaga
    18841 aattattgaa aatggtatag acagcgcaat gcagaaattc aatatgaagg gggacacacc
    18901 ttaacgtaag gtacgtgtac catataacta gtttatcagc aatgacaaat aaaggaaaaa
    18961 tatgaacaga attttgtttg accagtttca aagaacagaa aaattcaaaa agatttgttc
    19021 aaattataat aaggaaaaaa gccagcttat ccaaggactc aatgaggaag gattagctta
    19081 tttagcatgc aatcttttag atacatccga taaaatatta attctaacaa gcagtgaagc
    19141 aaaatcgaaa aagtacgaag agattattag agcttataca gagcattcag aacgccttca
    19201 gcctaaagaa tttattttgt ataatgttga tgctttaagc aaggatgtgg agtataaaag
    19261 ggcaaatctg ttaaataaca ttttaaataa gcggaaatgt attgtaactg cttcaattaa
    19321 ttcaataatt acaagagtaa tgtctaaaga tagatttaag gaaagcatta tagaattaaa
    19381 atatggcgac agttatgatt taaatgaatt aagaagcaat ttgattaagc ttaaatatga
    19441 gagagtggat gcaattgaag gggttggcca attctccgta agaggcggaa ttattgatat
    19501 tttttctcct tcagaagcaa atccgttcag ggtagaattt tttgatgatg aagtagattc
    19561 aataagatta atcgatttaa aaacacagcg ctcggttaag aacattaaat ctgctaagat
    19621 tcttccttgc agcgacatct tgtttaaaaa agaagaaata aaaattattt tatctgaaat
    19681 tgaaaaggat tattataagc agagtcaaaa ccaaagctta agcaaagaag cttttaagaa
    19741 tttaaagaac ctttacaaga gctatcaaga taagcttatg ggcggtatgg gtattgaaaa
    19801 tgccgacctt ttgataccat ttataaagga atctttttca aatattatag attattttga
    19861 agatgattct attcttcttc ttgatgagcc tgaaagaata tttgaagaac ttaatgattt
    19921 aaatgaaagc tttcagctga aatatactga attatttgaa agaggagaaa ttttttcaaa
    19981 tcaaagtgaa gtatatataa gcgagcatga actattgacg aagatttcca aaaaaccttt
    20041 catttctata aacggtaaag agaaaataat taaaacagat gattccgttc atatgctttt
    20101 taaagaagct ccttcttact acggcaagat tgaagagctg tcaaaagatt taaacaggct
    20161 gaaatacaag ggctataaag tagatattat actaagcacc tatgaaagct gcagtaaact
    20221 ttataattta ttaaatgatt atgaatgcac aaccacattg tctaaagata ccaacgcagc
    20281 agaaagcgga caagtggtaa ttgcaccggg agaattaaca aaaggatttg aatactacga
    20341 caataagctc cttgtgctta cagaaaatga ggtgcttggc tctgtcaagc gcaaatcccg
    20401 caagacgaaa aaacgcaagg gtgctgcaat tgaaattttc accgatttaa aagtaggaga
    20461 ttatgtagta catgagcatc atggaatcgg tcaatatata ggaattgaaa aaatagaagt
    20521 acagaatatt caaaaggatt atttgtgcat caagtataaa ggtgaagaca agttatatgt
    20581 tcctgttgac caaatgtctc ttatacaaaa gtatatcgga acagattccg aaaaacccaa
    20641 gcttaacaaa atgggcagct cagactggat aaaaaccaag gagcgctcaa aggcagccat
    20701 agaaaatatg gcaagtgagc tgattaaact gtatgctgaa agaaaggtat taaagggata
    20761 tgctttttca aaagataccg agtggcagaa agaatttgaa tataaatttc cttttgaaga
    20821 aacggaagac cagcttcgtt gcgtaaaaga aatcaaaaaa gatatggaaa agcctatttg
    20881 catggacaga cttctttgtg gcgatgtcgg gttcggaaaa accgaagtgg caatgagagc
    20941 cgcatttaaa gcagttatgg attcaaagca agtggcgatg ttggtaccta caaccatatt
    21001 ggcgcagcag cattatacaa atttagttga aagattcaga ggatatccca ttaaaattga
    21061 aatgctgagc cgttttcgaa cacattacca acaaaacaaa ataatcaacg atttaaataa
    21121 gggtttgatt gatattgtaa taggaacaca taaacttctt tccaaagagc ttaaatttaa
    21181 ggatttagga cttttgataa tagatgagga gcaaagattc ggtgtcagac ataaggaatt
    21241 gataaaacag cttaaatcaa atattgatgt tctgacgctg tcggcaacac caataccccg
    21301 aacacttcat atgtctatga ttggtgtaag ggacatgagc atcatatctg aaccgcccgg
    21361 agacagactt cctatacaaa cttatgttat tgaatataac gaaggattga taaaggatgc
    21421 aattgaaaag gaaatttcaa gaaagggtca ggtttattac gtacacaaca gagtggcaga
    21481 catagaccaa acagcagcaa agttgagaaa gctgcttcct gaggcacgaa tagcagtggc
    21541 tcacggccag atgagcgaaa gacatcttga aaatataatg cttgaatttg taaataagga
    21601 atatgatatt ttggtttgta caaccataat tgaaaccgga atggacatac ccaatgtaaa
    21661 tacattgatt attgataatg ccgattattt gggactttca caactgtatc agcttcgggg
    21721 aaggataggc aggtcaaaca aagttgcttt tgcatacctt acctacgaga aggacaaaat
    21781 gctgtctgaa gttgccgaca aaagacttaa ggcaataaag gaatttaccg agtttggctc
    21841 aggatttaaa atagctatgc gcgacttgga aataagaggc agcggaaata tattgggtcc
    21901 ggagcagcat ggtcatatgc tggcaatagg atatgattta tatgttaagt tcttagaccg
    21961 ggcagttaaa gaaattcaag gaaaacaagt tgaggaagat attgaaacgt cggttgattt
    22021 aaatgttgac ggttatattc cttcatcatt tattgaaaat gaagagcaaa aaatagaaat
    22081 ttacaaaaaa atagcagcag cagagaataa agatgatata tttgatataa ctgaagaaat
    22141 tattgacagg tttggcaata ttcctgttca agtagataat cttttaaaaa tatcatacat
    22201 taaatcattg agcaaaagaa ttaaaataaa atcaatagct caggcaggca ataccgtaaa
    22261 catagaaatg acgaaagatt acctgagtca agatatcatt gagtttttaa taaagaatta
    22321 ttatgaaaaa ataagctttg ttggaactaa cgaaccgatt attaaatata aactggattc
    22381 tcttgagcaa atgaaaattc ttagggaact ggaagagttt ttagaaacac taaataattt
    22441 taaagcagga aaaactgctg tggaggaaaa acatgaacaa atttaaaaaa tcattattat
    22501 taattacttt attatgcata gctttagtat ttacagcttg ctcaaactca ggcggcaaag
    22561 atgtcgataa agagggtgta atggcaactg taaacggcaa gccgattatg cagaaagatt
    22621 atgatgatgc attggcatat tataaggatt atgttgagta tcaattaggc gaagatgcat
    22681 gggaaacaga aacaccaagg ggtacttata aagaaattta tgaagaatac gtatgggatg
    22741 aaatgactaa atcattgctt ctgcttgatg ctgccgaaaa agaaggaata aaggcaagtg
    22801 aagaagaagt acagcattca ttggaaaact ttaaaattaa tttcgaaaac gatgaagaat
    22861 ataaaagttt tatggagcaa agaggtataa cggaagattt tattatacaa gagttaacca
    22921 aagaaatatt aattaataat tatataacat caaaaattga ttccttaaat ccaaataatg
    22981 aagagctgaa aaatctattt gaagaaatga aattaaatgt tctgataaaa gcaagccata
    23041 tacttgtgga tacggaagaa gaagctgtaa aaatagttga aagaattaat aagggtgaag
    23101 acttcggcga ccttgcaaaa gaattatcca ttgatacagc aagcggagca aacggaggag
    23161 atttggatta ctttaaatat acggatatgg ttaagccttt ctcagaagca gcatttgcac
    23221 tggaaatagg agaagtgagc cagcctgtac aatctgattt cggatatcat attattaagc
    23281 tgacagataa gattgttgat gaagaaacta ccgtagaatc tgaaaaagaa aagcttattc
    23341 aatattataa attgaacaaa tacgaagatc ttttagaaca gctgaaaaat gatgcggtaa
    23401 tagtaaaaaa ttaataattt gttaatttat gtaaaaaaat gttgatattt ttaattcctt
    23461 ccattataat gaattaatgt ttctttttta aaaatatttt caacattatt ttatctgttg
    23521 gataaaattt atgaaagtgt aaataataga aaatattaaa agaaatgtca taaaaatata
    23581 aaaatatgta attggaggaa cgtaagtaat gaaagcaaca ggaattgtca gaagaattga
    23641 cgacttaggc agagtagtta ttcctaaaga aataagaagg accttgagaa taagagaagg
    23701 cgatccttta gaaatattca ccgatagaga aggtgaaata atattgaaga aatattcacc
    23761 tattggagag ttgactgaat ttgcaggaga atatgtggaa tcattatttg aaactacgag
    23821 gcatattgca ataattacgg atagagacgg cgtaattgcc gtttcgggaa gctcaaagaa
    23881 agattatgcg gaaaaaaggc ttagtcctga attagaaaaa ataattgaaa gtagagaaat
    23941 gtatcaaacc agtgcaactt caaagccaat cagaattact gccaatgaat ttaatcctga
    24001 caattataca agtcaggtga ttgcaccgat tatggtacat ggagacccga taggagccgt
    24061 tatactctta tcgaaggata agggctctaa gatgtctgaa gttgaagaaa aattaataaa
    24121 aacagcaagt atatttttat cgagacaaat ggagaattga agatgcccta accccatatg
    24181 ttcgagctaa agagaagaac agatggttgg taggtcattc gcaacgaatt cccaatttat
    24241 gcgaccatag gtaaggggac acacctctac tatggagtta gtctttgagg gcatgaggaa
    24301 tgtccccaaa tataataaat tggtgaatga gactgcggta tatgcagatt gtctaatact
    24361 gataggtgtt tccaataatt tggaagcacc ttaaatattt ataaattaac actatatttt
    24421 gacaatgggt tttttggcac aagccattga cataaatgtg tttttttgtc atacttaata
    24481 taggttaata ttttttaaga cttttttatt tttaaccgga aagaggtata taatgaaaat
    24541 caggttttta ggtgcagtaa acggtgttac cggttcaaac cacttaattc agatcagggg
    24601 caaaaatatc atgcttgatt gcggtatgta tcaaggcaag gacgaggagc taaatgcaga
    24661 agaatttgca ataaatccgg cagatattga ttgcctttta ttgagccaca gtcatatcga
    24721 ccacagcgga agaattcctt tattgataaa aaaaggtttt aaaggagcca tttattgctc
    24781 aaaacctacc tatgaacttt gtgagataat gctcttggat tcggcacata ttcaagaggc
    24841 agaatcacag tggaaaaaca acaaggcaaa acgttcaggg agaaaattag tggagccctt
    24901 gtacacgcag caagatgtat tagatagctt gcagtatttc aaacctgtct tatacgatca
    24961 aattataaat attgatgaaa ttataactgt gaaatttaac gatgccggcc atattttagg
    25021 ctcttcaatc attgaaatat ggtttaaaga tgaaaatgat acaatgaagc tggtgtattc
    25081 cggagattta ggaatgcgtg aaaaaccaat tttaaaagac ccggctcaaa tagacaaggc
    25141 tgattattta ataatggaag caacttacgg caacagagtt cacgaaaaca tcgaaaaaag
    25201 aacagaagaa cttatcaata taatattaaa aacaactaaa aggggaggca gcgtaattat
    25261 cccgtccttt gcggtaggca gaacacagga gctgatttat gagctgaata aatattatga
    25321 cagccatttg aatgaaatcg gtactaagga aaatgaatta agaaaaatac cggtctatat
    25381 cgacagtcct cttgccatta aggcaacaga ggtattcaaa agaaacagct atgcttttga
    25441 taaggaagca aaggattata taatgaaagg ggacaacccc ttggattttg aaaatctgca
    25501 ttttactgtt actgctgatg aatccaaaaa attgaatttt tcaacagagc cgaaaattat
    25561 tatttctgcc agcggaatgt gtgatgcagg aagaataaga catcatttga aacataatct
    25621 ctggagaaag gaagcaagca ttgtatttgt aggctatcaa gctgaaggaa cactaggcag
    25681 aaggttattg gacggtgaaa aatgtgttaa attatttggt gaagatatag tggttaatgc
    25741 tgaaatttac aatgttgaag ggttttcggg acatgccgat aagcctgctc ttctcaactg
    25801 gattggcagc tttaaagaaa agccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnaattc agaaagatta acaacaatat taaaagtctt gaaaatgaaa ttatgaattt
    25981 aactatgatt agtgcaaaat agatttctag aaaaaagtta taggaggaag aaaatgaaag
    26041 ttttaatgac agggaatgaa gcagttgctc agggtgctta tgaagcagga attcattttg
    26101 ctgcagcata tcccggaaca cctagtactg aaatactgga aaacatagct cttcgcaagg
    26161 aaataacagc agaatgggca ccaaatgaaa aagtggcagt tgagtcggca tttggggcaa
    26221 gccttgcagg agcaagaact ttgacagcta tgaaacatgt tgggcttaat gttgcagctg
    26281 acccgttttt cacaatcggt tacacaggtg taaacggtgg aatggtactg gtaagcgcag
    26341 atgacccggg acttcattct tctcaaaatg agcaggataa caggtattac gcaagaatgg
    26401 caaaggtagc tatgcttgag ccaagcaact cacaggaatg caaggacatg gtaataaaag
    26461 ccatggaaat aagcgaaaag tatgatgtct tggtaatgct tagaatgact acaagagttt
    26521 gtcacagtaa atcaattgtt gaaacaggcg aaagaataga agttcctgta aaaccatacg
    26581 tgaaaaatgc gccaaaatat attatggcac ctgctaatgc aaaagcggca catgtggtag
    26641 ttgagaaaaa gcttaaagaa cttgaaaagt tttcgaatga aactgagttg aattatatag
    26701 aatggaatga caaaaaaatc ggtgttattt cggccggagt tgcttatcaa tatgcaaaag
    26761 aagtattcgg aaaggatgca tcatatttaa aattaggatt tactttcccg cttccaatgg
    26821 aaaaaattaa aaaatttgca gaagaggttg aaactcttta tgtagttgaa gaattagaac
    26881 cttttatgga agaacagata aaagctgccg gtattaaatg tatcggtaaa gaaaaaatcc
    26941 ccaatatatg ggagcttaat ccggatattg taaaaaaatc aattttcggt actgaaaatg
    27001 aaataattga ttatgacaga acaatagcag tgggaagacc tcctacactt tgtgccggtt
    27061 gtcctcaccg tgcattctat tatcaattgt caaagaaaaa gaacactata gtaacaggtg
    27121 atataggatg ttatacattg ggcggagcac ctccgttaaa ttcaatggat acatgtgtat
    27181 gtatgggggc aagcataagc atggcacatg gtgctaaaaa ggcatttgaa aggaacaatg
    27241 atgataaaag agtagttgca gttataggag attcaacatt cttccattca gggatgacaa
    27301 gcttgctgga cgtggtgttt aacaaaggaa ataccataac ttgtatttta gataatagaa
    27361 taacggcgat gacaggacat caggataatc cgggaacagg ctatacactt cagggagata
    27421 gtactgatgt ggttcaaatt gagcctatag tcagagcatt aggtgttaaa aacataaaaa
    27481 cagtaaatcc gatgaatcaa gctgaaatga aagctgctct taattgggct tacgatttaa
    27541 acgaaccatc ggtaataatt tcaagatacc catgtgctct taagaaatat acaaaagaag
    27601 acctggaaga attcggacct attcaaggtt attgcgaagt tgacgaagaa acatgtatag
    27661 gatgtaaaat ttgcgtaggt acaggttgtc cggcaatagt gtttaaaaaa gacattaaga
    27721 aatcttcaat aaataaattt caatgtgtcg gttgtgagct ttgtctacag ttatgcccca
    27781 ctaaggcaat cagtaaggta ggtgaataaa atgaaggata ctaaaaatat tttattggta
    27841 ggtgttggcg gtcagggaac aatattggta agtaaaatat tatcaacagg tcttatgaca
    27901 gccggttatg acgttaaaat gtcagaaatt cacggaatgg cacaaagagg aggcagtgtt
    27961 tcgactcagg taagatatgg aagtgaagta ttttcgccta ttatcggaat cggtgaagcc
    28021 gatattctgg tttcctttga aacgatggaa acattgagat ggcttgaata cttaaagcct
    28081 gacggagttg ttgtaatcaa tgatttcgaa attgcttccg cacccatatt gatgggaaaa
    28141 gccgaatatc ccgagggaat aatcgaattg attaaaggga aagctaaaac aaaggttata
    28201 aatgccggag aaacagctga aaaactaggt aatcagaaag ttatgaacgt ggttttatta
    28261 ggggccttga ttaagtcgat gaacttaacc gatattgatt gggaagctgt aataaaaagc
    28321 caggtaaaag aaaaatttat agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28441 nnnatcctat ttaaaagaaa agcaatttga cattaaggaa ttatcttgtg ttgttgcaag
    28501 agggggaatg cttcttccgg taaaatcggg agcatatgtt attaatgata ttatgctcga
    28561 cagggtggaa aatcgtcccg taatggagca tgcatcaaat cttgcagctc cgattgccta
    28621 cggtattgca aaggaggccg gcgtaacttc atatatttat gactccgtga tgacagatga
    28681 atttcctgac atttctcgta tatccggcat gcctgatata cccagaaaaa gcgcaagcca
    28741 tgttttaaat acaagggcaa tggctataaa atacgctaaa agtatcggca aaagttacga
    28801 agagctgaac actatcgttg cgcatttagg aggaggaatt tcaatcaata ttcatcacaa
    28861 gggtcagata atagacttgg tttctgatga tgaaggaccc ttttcacctg aaagagcagg
    28921 aagagtaccg tgcagagagc tgataaatct ttgttattca ggtaaatttg ataaaaaaac
    28981 aatgcagcga aagctaagag gaagcggagg cttaaaagca tatttaaaca ccgttgatgc
    29041 aagagaagtc gaatctatga ttaaagaggg caatgaatat gcaaaattga tttatgaagc
    29101 aatggcatat caaatagcaa aaggaatagg agaactggct acagtggtag aaggcaaggt
    29161 agatgttata attcttaccg gaggtattgc acattctgag atgcttactt cctggataaa
    29221 aaacagagta tcttttatat ctcatgtaga aataatgccc ggtgaaaatg aaatggaagc
    29281 tttggctttc ggcgctctaa gagttctcag aggtgaagaa aaggctagag agtatattga
    29341 ataaaaaagg aagggtaata ataaaaacag taatcgtaag gttactgttt ttttataatt
    29401 aacccccatt ccctttatta attggaatat tttgcaaggt tcataatatt acgaatataa
    29461 aggaacatgt ttaattaata attcggtata gtacgaacaa aaaatatatt ttttgttgaa
    29521 attactatat aaaccgttga caaggatttt tatatttgtt attataattt tgtaaaatga
    29581 taaaaaggaa ggtttattta tgaactttga cgttaagagg gtgtttgttg aaaaaagagc
    29641 agggtttaac accgaagcat taaatttatt aaataactta aaggaaaatt taaatattga
    29701 aaaattaaat aaccttagaa tattaaacag atatacggta tcaaatattg atgaagatga
    29761 ttttaaaaaa agtgtaaata ctgttttttc agagcctaat caggatattg tgtattttga
    29821 agatgttgag cttgaaggac atgtttttgc cgttgaatac ctgccaggac aatatgacca
    29881 aagagccgat tttgcggctc aatgcataca aatagtcacc ggaaaagaaa aacctaccgt
    29941 taaatatgca cgagtttttg ttcttgaagg ggatttatca aaagatgata ttgatagaat
    30001 taaaaaatac tacattaacc ctgttgattc cagagaagcg cagttagata aaccgcatac
    30061 tttggatgat gaatatccga tggcagagga tgtacaaaca ataaacttaa taaataaatc
    30121 taaacaagaa ttagaagagt tccgaaatga tatagggctt gcaatgagct ttgaagattt
    30181 agctttttgt caggaatact ttaaaaatat tgaaaaaaga aatccgacaa ttaccgaaat
    30241 taaagttatt gatacctatt ggtcagacca ctgcagacat acaacatttt ctacgattat
    30301 agaaaatatt gaatttgaaa aaggaaaaat aactgaagaa ataaaaaaca catatgatga
    30361 gtatttgaat gtaagaaagt atgtttatga aggcaaaaaa gatatcacat taatggacct
    30421 ggcaacaata ggtgcaaagg aattaagaaa agcaggtctt ttggatgact tggaaatatc
    30481 ggaagaaatc aatgcgtgca gcattataat aaatgcagat atagacggga aaaatgaaga
    30541 gtggctttta atgtttaaaa acgaaacaca taaccatcca acagaaatcg agcccttcgg
    30601 aggagcagcc acatgccttg ggggagccat aagagaccct ctgtcagggc gttcatatgt
    30661 atatcaggca atgcgtgtta caggaagtgc tgaccccaga caaaaaattg aagatacatt
    30721 accgggaaag cttcctcaaa ggaagattac tacggaagca gcagcaggat acagctccta
    30781 cggaaatcaa atcggacttg ccaccggttt tgtaaatgaa atatatcatt ctgattatgt
    30841 tgctaagaga atggaggttg gtgcagtcat tgctgcagca cccagagaaa atgtaaaaag
    30901 agaaactcct aaaaaaggtg atctcgtaat actattggga ggaagaacgg gcagagacgg
    30961 tataggggga gcaaccggtt catccatgga gcatacggaa gaatcaatat tgttaagagg
    31021 ttctgaagtt caaaagggta atgctcctac ggaaagaaaa attcaaagac tgttcagaaa
    31081 aaaagaagta agcacactta taaaaaaatg caatgatttt ggtgcaggag gagtttctgt
    31141 tgccatagga gaattagcag acggtttaaa cataaatttg gacttggtgc caaaaaaata
    31201 tgacggactt gacggaactg aattagctat ttccgaatcc caggaaagaa tggctcttgt
    31261 tgtaagaaaa gaagatgcag ataaatttaa tgaatatgct aatgaagaaa accttgagtc
    31321 aacagtggtt gcagaggtta ctgatgacag aaggcttaaa atgacatgga gaggaaagac
    31381 gatacttgat attagcaggg attttttgga taccaacggt gtaaagcaaa aaacaaatgc
    31441 aagaataaaa gaacctctta actcaaaata taaaagaagt tttaaaagta ataatataag
    31501 agaaatatgg cttgaaaatt taaatgattt gaatgttgca agtcaaaaag gaatgattga
    31561 aagatttgat tcgacagtag gtgccggctc agtattagtg ccccttggag gaaagacaca
    31621 gcttactcct gcagaaggaa tggccgccaa aattcctgtg gaagaaggaa ttacaaatac
    31681 ctgttcgctg atgacctatg gatttgaccc ttatctttct aaggaaagcc cttaccacgg
    31741 agcaatgtac gcggtaatac attctattgc caaaattgta gcaatgggag gagactataa
    31801 aaatattagg ctatcattcc aagagtacta tgaaaagttg aataaagagc ctgaaagatg
    31861 gggaaaaccg ttaagcgcct tgttgggagc atttaaggtc cagcatgaac ttaaaattcc
    31921 ctcaataggc ggaaaggaca gtatgtcagg tacatttaac gaactcaatg taccgcctac
    31981 gctaataagc tttgcagtaa cagtttcaga tgtaaaaaat attatttcac cggaattcaa
    32041 gagagcaaac aacaaggttg tattaataaa gattaaaaaa gatgaaaata atattcctga
    32101 ttttgaagat atcggtataa aatattcggc tattcatgaa gcaataaaaa caggaaaggt
    32161 tttaagtgcg cataccgtag gatacggggg gattgctgaa gccataagca agatgacctt
    32221 gggaaatgaa ataggattca gaagtgattt tacaattggt aatgattggt ttgactcaag
    32281 ctatggcgac attgtattag aggtcgacaa ctcattttta ccatttgcgg attgcattga
    32341 aataggaaaa acaataaatg aaaaggaaat atgggttaat ggtatttgca tatccttgga
    32401 agaaatcaag actaattggc ttaaaccttt aaataatata ttccctgtga aaaaggatgg
    32461 agccggagag attgagaact atagttttaa ttctaaacat attgctaaat cctccatatc
    32521 cattgcaaaa ccaagagtat ttattccggt attcccggga acaaattgcg aatatgaaac
    32581 caagagagcc tttgaaagag ccggagcaaa agcagatatt tttgttttta aaaatttaag
    32641 ctcacaggat attaaagatt caataacaga aatgaattca ctcataaggc agtcacagat
    32701 tattgcattt ccaggaggat tcagtgccgg agatgaacct gaaggctccg gaaagtttat
    32761 tgcggcagta ttcaggaatg aattgttaaa ggatgcagtt acagatttgt taaacaatag
    32821 agacggcttg attattggta tatgcaacgg cttccaggca ctcattaaat tgggattggt
    32881 accatttggc gaaatcactg atatggagaa tgattatccg actttaactt ataataggat
    32941 aggaaggcat atagcaacat ttgttaaaac aaaggttgtg agcaacctat ccccgtggtt
    33001 aggaatgtgc agtgtgggag acacccacta tttgcctgtg tctcacggag aaggaggatt
    33061 tgcagcaaat caaaaatgga tagaaaaatt gaaaacaaat ggtcaaatag caactcaata
    33121 tgttgattta aataataatc cgacctatga cggtttttac aatccgaacg gctctgtgga
    33181 agcaattgaa ggaataacaa gccctgacgg aagggttttc ggcaagatgg cacattctga
    33241 aagactggac gaaaatttat ataaaaatat tcccggaaac aaaaaccaac cgatatttga
    33301 atcaggcgta aaatatttta aataatggag gagtaatgaa agtagcaata gttatgggaa
    33361 gtgattcaga ctttccggta gttgaaaagg gttataaagt tttgaaagat ttcgaagtta
    33421 tggctgatgt aagggttata tcagcccaca gaacaccata tgaagctttg gaatttgcaa
    33481 gaaatgcata taaaaacggt tatgatgtca ttatagctgc agcaggaaag gctgcccatt
    33541 tgccgggagt gttggcagga tgcacgactg tgccggtaat aggacttccg attaaatctt
    33601 cggaattaga cggaatggat gccttgttat cgatagtaca gatgcctccg ggagtgccgg
    33661 ttgcaacagt gggcataaat gccgcagaaa atgctgcatt actggctatc cagatattat
    33721 cggtgaaata cgaagattta aggaataagt ttttggatta taaaaaacaa atggctgaaa
    33781 aagtatttga aaaagacaag aaattaaacg aaaaattgat gtcatcctga gcacaggata
    33841 aagatgacga gacaaacatt cataccaatg acaagaagga gatactatgt tcaatttaga
    33901 agaagataaa ttacatgagg aatgcggggt tgttggagta tattcagaca atccggatat
    33961 tacaagtcag cttatatatt acggtttatt tgcacttcag catagaggcc aggaaagtgc
    34021 aggtattgca attcatacag gaaaaagaat tgaattccat aaggatatgg gattggttgc
    34081 cgatgttctt tcacaaaagg taatagatag tttaaaaggc aatattgcaa tagggcatga
    34141 cagatattcc acatccggag aaagcgaaaa gcaaaatgca caacccttag tagtgcgata
    34201 taaagaaggt tcgatagcct tagcccataa cggaaatttg gttaatgcag attctataag
    34261 agaaatactt gaaaatgaag gagtaatatt tcagacttcc actgattctg aggttgtagc
    34321 caatttaatt tcaagacatt acagaggcag tataattaaa tctatacaaa ctacaatgga
    34381 ggttgtaaaa ggagcatatg catttgtagt tatgacagat aaagagctta tcggcgtaag
    34441 ggacaactat ggccttagac ctctttgcct tgggaaaagg gaagacggat atttgcttgc
    34501 ttcggaaagc tgcggaatta ctgcaatggg cggtgaattt ataagagata tagagcctgg
    34561 tgaaattgtt gtaataaatg aagaaggagt taacagcata aaaaccacca aatatgcaca
    34621 acaaagattg tgtatattcg agcatgtata ttttgcaaga ccggacagca atttagatgg
    34681 tgtaaatgtt tatgagacga gatataaatc aggtagattg ttggcacagg aagctcccgt
    34741 gaaagcagat ctggtatttt gcgtgcctga ctcaggaaca ccggctgcat tgggatatgc
    34801 agaagaatta agtattccat atggtcttgg aattataaaa aacagatatg caaagaggac
    34861 tttcattgag cccactgata aactaagaca gcagggagtc aaaacaaaat taagcatttt
    34921 aaaggaaatt gttcaggata aagttgtagt tatggttgat gacagtatag taagagggac
    34981 aacaacaaaa caaattgtaa gtatggtcag ggaagcaggg gcaaaagagg ttcatgtacg
    35041 aatagcttca cctccggtaa cccattcttg tttcttcggt ataaacaccc ctgacagaaa
    35101 agaattgtta ggcgcaaata aaacaataga agaaataaaa gactatatta aagcagacag
    35161 cgtacatttt ttgagtttag aaaacttaat caaatctaca ggttcaaatc taggctactg
    35221 caaggcttgt ttaaacggcg attatcccat ggaagtaccc aataacggta tataaggagg
    35281 tattatgact gataaactga cttataaaga ttcaggcgtg gatgtaaatt ccggttatga
    35341 aacggtaaag ctcattaaag agcatgtaag aaaaacacat ataaagggtg taatttcaaa
    35401 cataggcggt tttggaggaa tgtttgaaat cgataaggat gaatatgaag aaccggtatt
    35461 ggtttcggga acagacggtg ttggaacaaa gctcatgatt gcatttatgg cagacaagca
    35521 cgacactatc ggtgaggacg ccgtagccat gtgtgtaaat gatgttatat gccagggtgc
    35581 aaaaccttta tttttcctgg attatatagc aacaggaaaa ctagagccaa aaaaagcagc
    35641 aagcattgtt aaaggtgtat cggagggctg cattaaggca ggctgtgcct taattggagg
    35701 agaaactgcc gaaatgccgg gactttacga cgtgggagaa tatgatattg cgggattttg
    35761 tgttggaatt gttgataaaa agaaaataat cgacggctca aaaataatag aaggggatat
    35821 tttaataggt attccttcaa gcggaattca cagcaacgga tactccttag tgcgaaaagt
    35881 ttttttcagt cacaaaaact atgatattac agactatgta gaggagcttg gaaaaacctt
    35941 gggagaagag ttattaactc cgacaagaat ttatgccaag ctggtacttg acctcatatc
    36001 taaatttgaa ataaagggtt tatctaatat tacaggcggg ggattttatg aaaatattcc
    36061 aaggatgttt cctcaaggca ttacagcaga tatagatact aaaaatatca atgttcttcc
    36121 catatttaag ctgttgcaaa aaaaagggaa cattgacatt gatgaaatgt atggtacatt
    36181 taacatgggt atcggcatag tattgactgt tgcaaaagat gatgcggacg atattttgag
    36241 ctacctgaaa agtgtcggtg aaacccctgt aataatagga agaacagtaa aaagggaagg
    36301 cgggcttaaa ttatgccaca ggtaaaaatc ggtgtattaa tttcaggctc aggaactaat
    36361 ctgcagtcct tgattgataa tatcgagaca ggaaatataa agggtaaaat taccgttgtc
    36421 atttcaaaca gaaaagatgc ttatggactt aacagagcaa ggcaaaaaaa catcgatgcc
    36481 ctatatataa ggcaaaagga ttatgaaagc tttgaaaatt tcaatgatgc tattattgat
    36541 gagttaaaaa aacataatgt tgaattggtg gtactggcag gatatctaaa aatactaact
    36601 tctaaattta ttgaagagta taggaataga ataattaata ttcatccgtc tttaataccg
    36661 gcattttgcg ggaaaggcta ttacggtatt aaggttcatg aagaagttat taaatacggc
    36721 gttaaattaa gcggtgcaac agtacatttt gtagatgagg aagcagacac gggtccaatt
    36781 atcattcaag aagcggttga agtaggctat gaagatactg ccgaaacatt acagcaaaaa
    36841 gtacttaaaa tagagcataa aatacttcct ttagctgtga agtattactg cgaagggaaa
    36901 attcaaataa gcggaagaaa agtaaaaatt aaggagtaaa atgatgagag ctttaattag
    36961 tgtatccgat aaaacaggaa tagtcgaatt tgctaaagaa ttagaaagat taggagtcga
    37021 gattatatca acgggaggca ctcacaaaaa attaaatgag gcaggaatta aagtaagagg
    37081 aatttccgaa gtaacaaatt ttcctgaatg cctcgacgga agggtaaaaa cgttgcaccc
    37141 gaacattcat gcaggattac tggcaatgcg ctcaaaccct gagcatatga aacagctatt
    37201 agatttaaat attaagccaa tagacctggt ggttgtaaat ttgtatccct ttaaacaaac
    37261 tgttttaaaa gaaggcgtaa gcagggaaga agcaattgaa aatattgata taggcggtcc
    37321 tacgatgctt cgctccgcag ctaaaaacta tcaggatgtg gctgttatag ttgaccctaa
    37381 ggattatgaa ttggttctta atgaattaaa agaaaataaa gaagtttcct tagacactaa
    37441 attttatctt tgctcaaagg ttttttctca tactgctcat tatgacacat tgattgcatc
    37501 ttatattaaa agagaaagaa aggacaatac tttgcctgat acatacacaa tgacttttga
    37561 aaaaatccag gaaatgcgat atggtgaaaa tcctcaccaa aaagcagtat tttataaaga
    37621 agttaaaaat gttgacggtc ttttggcttc agctttgcag cttcacggca aggagttgtc
    37681 ttttaataat ataaatgata ccaacggagc tttggaactc ttaaaagaat ttgacgagcc
    37741 ttgtgtcgtt gcatgcaagc atgcaaatcc atgcggagta ggatgcgggg acaatattta
    37801 tgaagcttac atgaaggcat tcaatgcaga ccccacttca atatttggag gcatagttgt
    37861 atttaacagg gaaattgatg aaaggactgc acaggaaatc aataaaatct ttgttgaaat
    37921 aattgttgct ccttcataca gccaagaagc tttgtcagta cttatgtcaa agaaaaatat
    37981 aagacttttg caaatagaaa atatattaca aaagcaggat gaatattcct acgatattaa
    38041 aaaggttgcc ggcggtttat tagtccagga aattgattca aagctccttc ctaaaggtga
    38101 agagttaaaa acagtaactt cctctaaacc gaccgaaaag gaaatgaagg atttatttat
    38161 ggcatggaaa attgtaaagc atacaaaatc caatgccata gtcattgtta aagacggaca
    38221 gtcccttgga atcgggccgg gacaggtaaa ccgcatatgg gcatgcaagc aggcaattga
    38281 gcatgcgtat gattttttgg gaaaagacag caccaaggga gcatcccttg catcggatgc
    38341 atttttcccg ttccccgatt gtgcggagga agcggcaaag gccggaataa gagctattat
    38401 tcagccgggc ggctcattaa aggatgatga atcaatagaa gtgtgcaaca aatacaacat
    38461 atccatggtt ttcaccggta tgagacactt taaacattag gaggtcttat gaaaattctt
    38521 gtagttggaa gcggagcaag agagcataca atttgctgga aattaaaaca aagcagcaaa
    38581 ctgtccaagt tatactgtgc tcccggaaat gcaggcatat ctaaaattgc agaatgtgtt
    38641 gatattaaag cagatgaact ggagaagtta gttgaatatt cggtaaaaaa cagtatcgat
    38701 ttggtagttg tcggccctga agtgccattg gtagcagggt tggtcgacat gcttaacgag
    38761 aaaggaataa aggcattcgg acctgtaaaa gccggagcaa aatttgaagg gagcaaatcc
    38821 tattcaaagg atttcatgaa aaaatactca ataccatctg ctaattatga aatttatacg
    38881 gatagtgaaa aagcaatcag tgaacttaac aaatttaata ctccgattgt tgtaaaggct
    38941 gacggtttag ctgcaggtaa aggtgtttta atatgtgaaa cacatgaaga agccaaagaa
    39001 gccattaaaa gtataatggt taaaaagctc ttcggcgaag caggaaattc agttgttata
    39061 gaagaattct taaaaggtac ggaagcttcg ctactatgct ttgttgatgg caaaaatatg
    39121 attcccatgg aaagtgcaag agattacaaa aaagcatatg accaggacaa agggctcaat
    39181 acgggaggaa tgggatgctt ttctccaaac accatattta ccgaagaatt aaataaatac
    39241 attaaagaaa atatattaga caggactctg aatggttttc tgaatgaaaa tattgaattt
    39301 aaaggagttt tgtttatagg tctcatgatt gaaaacaatc aagctaaggt attagaatac
    39361 aacgctcgtt ttggagaccc tgaaacagaa gtagttcttc caagacttaa gtccgattta
    39421 attgatataa tgcttaaatg tattgagggc aatttaacag aagatgattt gcagtgggaa
    39481 gataataaat gtgtaaccgt aatattagcc tcgggagggt atcccgaaag ctatgaaaaa
    39541 ggcaaggaaa taacaggaat aaacgaggtt gatgatgata taataatttt ccatggaggg
    39601 accaagttat cagggaataa aacagttaca gacggaggaa gagttttggc agtaacagcc
    39661 atgggaaaat ccttagagga agcgcgaaaa aaagtatata aaaacattcc caaaattaat
    39721 tttgaaaaaa tggaatatag aaccgacatt gctaaaatct aaaattat
//
LOCUS       DAOSHP010000007        39456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_783_length_39456_cov_9.645669, whole genome shotgun sequence.
ACCESSION   DAOSHP010000007 DAOSHP010000000
VERSION     DAOSHP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 39456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39456
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_783_length_39456_cov_9.645669"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLB33_01475"
     CDS             complement(<1..512)
                     /locus_tag="PLB33_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG62220.1"
                     /translation="MKIFRSHVLVCGGTGCSSSNSAQIRQRFIDKIKEVNLDNEVQVI
                     GTGCFGLCEAGPVVIIYPEGSFYARMTVDRVDEIVEEHLLKGRIVTKYLYDESVKEEK
                     LKALSEVNFYKKQKRVALRNCGLINPENINECIARDGYMALGKVLTEMTPDEVIKLVK
                     DSGLKGRGGAG"
     gene            complement(534..899)
                     /locus_tag="PLB33_01480"
     CDS             complement(534..899)
                     /locus_tag="PLB33_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOG62221.1"
                     /translation="MKSLEELKKLRDESIKNIEMRNTDKDVRVVVGMATCGISAGARP
                     VLATFVEEVAKRNLNNVSVVQTGCIGMCTYEPIVEIYAPGKEKVTYIHVDSEKAKRIV
                     SEHLANNRVVVEYTIGSEN"
     gene            complement(909..1394)
                     /gene="nuoE"
                     /locus_tag="PLB33_01485"
     CDS             complement(909..1394)
                     /gene="nuoE"
                     /locus_tag="PLB33_01485"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOG62222.1"
                     /translation="MTKALDVKANEKLFIELKEYIDSVKDVDGSLMQVLHKAQDTFGY
                     LPIEVQKFISSELDVPLAEVYGVATFYTQFAIEPKGKHKIGVCLGTACYVRGAQLIMD
                     KLTKELNVKVGGTTQDNLFTFEATRCLGCCGLAPVMMIDQDVYGKLEPKKIPEILAKY
                     K"
     gene            complement(1737..2582)
                     /locus_tag="PLB33_01490"
     CDS             complement(1737..2582)
                     /locus_tag="PLB33_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOG62223.1"
                     /translation="MAAKRKALGKGLSALIPEDLEKEDYEKIHEIDIDLISPNPNQPR
                     KLFEQDKLDELTESIKKYGVIQPIILRKDGELYTIIAGERRWRACKNANIKTIPSIVR
                     DIENKNASELALIENIQREDLNPIDEANAYQFIMDRYGITQEQVSNIVGKSRVYVTNI
                     MRLINLDEYVKDKIIKNLISAGHGRAILSLDKEKQKSLTDIIIRDNLSVRDVEKLVRS
                     SKKPRRREKPVKDKYIVYLEELLTERFSTRVNIKNKKDKGKIEIEYTSSDDLNRILDL
                     LKIED"
     gene            complement(2597..3358)
                     /locus_tag="PLB33_01495"
     CDS             complement(2597..3358)
                     /locus_tag="PLB33_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG62224.1"
                     /translation="MTKVISIFNQKGGVGKTTTNVNLCTALALKGKKVLSIDIDPQGN
                     TTSGFGFNKNKLEYTIYNVLIEDYDINKIIFKTEIENLDITPANIQLAGAEIELTNTK
                     FREKTLREKINLIENKYDFIMVDCPPSLGLLSLNALTASNTVLIPIQCEYYSLEGVGQ
                     LIDTVKLVKKNLNPKLEIEGVILNMFDGRTNLSIQVVEEVKKYFKDKVYRTVIPRSIR
                     LAEAPSFGQPIMLYDDKSKGSIAYIKLSEEFIKNN"
     gene            complement(3370..4158)
                     /gene="noc"
                     /locus_tag="PLB33_01500"
     CDS             complement(3370..4158)
                     /gene="noc"
                     /locus_tag="PLB33_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HOG62225.1"
                     /translation="MISNIVHIDVNKIIPNKNQPRKTFDEKALKELSQSIDNYGIIQP
                     ITVRKIYDDIYEIVAGERRYKAVKMLNMDTIPAVIIEAKEEESAAMSLIENLQREDLH
                     YLEEAYAYERLINEFGISQTQLAQKLGKSQSAIANKVRILKLPESVKEKLREGNLTER
                     HGRALLKINDEEILLNIVEKVIKKDLNVSETEKIVDSIADELNIKKKADKRYVRNFIN
                     YKIYINTIKNAYNEIVKTGIDAKFEQNESDDYIEIKVKIPKKSM"
     gene            complement(4169..4882)
                     /gene="rsmG"
                     /locus_tag="PLB33_01505"
     CDS             complement(4169..4882)
                     /gene="rsmG"
                     /locus_tag="PLB33_01505"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOG62226.1"
                     /translation="MIKTLEEGFKELKLPYSKDIENNFIIYRDLLKEWNKKINITSIE
                     NDGEIYIKHFIDSVLLLNENNINENKTIIDVGTGGGFPGLPLKIVNNNYKITLLDSLR
                     KRIDFLSEVVKALNLKDVKLIHGRAEDYGQNKEYRECYDICVSRAVAPLNVLSEYCIP
                     FVKVGGYFAAYKSDNISQEISSSDNAIKKLGGEIKEIKKINLPGTDIIRKIVIIEKTD
                     KTEIKYPRKAGKPAKDPLK"
     gene            complement(4893..6782)
                     /gene="mnmG"
                     /locus_tag="PLB33_01510"
     CDS             complement(4893..6782)
                     /gene="mnmG"
                     /locus_tag="PLB33_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOG62227.1"
                     /translation="MEDRVREFLEESIDVLVIGAGHAGCEASLATARLGLKTIMLTLS
                     LDSIAMMPCNPNIGGTAKGHLVREIDALGGEMALNIDKTMIQCKMLNRSKGPAVHSLR
                     AQADKKKYHREMKKVIENQKNLRVIQTEATKIKVNEDKSFNVYTKLGAYYRARAVIVT
                     TGTYLKGRIYMGELNYSSGPDGLAPANELSDSLMDLGIELIKFKTGTPARIHKDSINY
                     EKMTIQTGDDEIIPFSFMNDEINIEQVPCYITYTTQQTHELIRNNLDRSPLYGGVIKS
                     VGPRYCPSLEDKVVKFSDKEKHQLFVEPEGLDTKEMYIQGMSTSLPYEVQIQMYRTMI
                     GLENAEIMRPAYAIEYDAIDPTQLKPSLELISIKNLFFAGQINGSSGYEEAAAQGLIA
                     GINAYLSLNNKEPFILDRSEAYIGVLIDDLVTKGTTEPYRMMTSRAEYRLLLRQDNAD
                     ERLTEKGYKIGLVTEERYKRFKDKRQRIENEINRLKNLKITPKADTNKALEDMGSAAL
                     KIPTTLYDLLRRTELNYDNTLFLDPKRPVLRKEIKDYVETVIKYEGYIQKQIAQVEQF
                     KKLENKKIPEDINYDDVKSLRIEARQKLNKIRPNSIGQASRISGVSPADINVLLIYLM
                     TYNKE"
     gene            complement(6796..8169)
                     /gene="mnmE"
                     /locus_tag="PLB33_01515"
     CDS             complement(6796..8169)
                     /gene="mnmE"
                     /locus_tag="PLB33_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOG62228.1"
                     /translation="MFNDTIAAIATFPGNAGINIIRLSGNDALNIAYKIFVDKNKRKP
                     DNLKPRYLHYGFIVDKDKKVIDEVLLSYMKAPNTYTREDIIEINCHGGIISAKKILDT
                     VLHYGCRTAERGEFTKRAFLNGRIDLTQAEAVIDIINSKTESSHEISIKHLEGRLSKQ
                     INAVIDEIMELLANIEVNIDFPEYDEDEITIEKVKALCHNLVPELDKLINTADTGKII
                     KEGIKTVILGKPNVGKSSLMNFLLNENRAIVTEVPGTTRDTIEEYVNIKGVPLRIIDT
                     AGIRETDDMVERIGVEKALEKVKEADLVIMIFDSSKELEKEDESILDYIKDKKVIYIR
                     NKTDLESRLDLSSYKKIEEEIINVSILQNQGLDDIINKIYSLFFKGRLDIRDELIINN
                     VRHKNLLIKAKNNIEEVLKSIENNMPIDFIEIDLMQAMENLGLIVGKSVSDDLIDKIF
                     DEFCIGK"
     gene            complement(8317..8940)
                     /gene="jag"
                     /locus_tag="PLB33_01520"
     CDS             complement(8317..8940)
                     /gene="jag"
                     /locus_tag="PLB33_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOG62229.1"
                     /translation="MRNVTIEKPTVEEAIAAALKELRAENNEVEIEIINEPSKGFFGL
                     GSKDAKVKVTITNGPEERARKFIDILLKKMSIEADYEVNYSDNILNVDIVKIGEDDKG
                     IVIGKRGKNLDEIQFLLNLMVNKGRENYVRVIFNVEDYRAKREETLKRLALKMADKCR
                     YYNRKVKLEPMNPYERRIIHSTLQDVKGIITYSEGEEPYRKVVIDQK"
     gene            complement(8941..9822)
                     /locus_tag="PLB33_01525"
     CDS             complement(8941..9822)
                     /locus_tag="PLB33_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOG62230.1"
                     /translation="MNLDFIAVPMGMLVKLVYNMVSVVDSKYLSAYSIAIIISTILFK
                     LIVLPLTLKQSKSMKKMQELSPKIQELQQKYGKDPQTLQRKQMELYKEANYSPFSGCL
                     PLLIQFPILIAFFYVIREPVKYVFGDQAFYDSINRAFLWIKDLNVNENTVWFLKNNDW
                     IVLTNAIKEEFSISSSMHLNQVMEVLNAQDIMVNGLSMGGMTLPFLGSALPVLAVITG
                     ITTYLTTKMTAQPSINEQQASTQKTMNMMMPIMIFVFSIQFPAGLALYWVISNLFQLV
                     QQFIVLNSSKNPKEELK"
     gene            complement(9833..10042)
                     /gene="yidD"
                     /locus_tag="PLB33_01530"
     CDS             complement(9833..10042)
                     /gene="yidD"
                     /locus_tag="PLB33_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOG62231.1"
                     /translation="MSKVLIFIIRIYQKFSSKSAVRHCRFYPTCSQYFVEALQKYGFL
                     KGSYLGIRRILKCHPFNPGGYDPLK"
     gene            complement(10039..10374)
                     /gene="rnpA"
                     /locus_tag="PLB33_01535"
     CDS             complement(10039..10374)
                     /gene="rnpA"
                     /locus_tag="PLB33_01535"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOG62232.1"
                     /translation="MIIIKKNNDYKAVYNCNNSISDNNLVLFIKKNNYNYNRFGFTTA
                     KKIKKAVHRNVIRRRLKEIVRANEHYLEEGYDFVLMARINAVDSDYKSLESSFIKLMK
                     RKKIYRYTI"
     gene            complement(10424..10558)
                     /gene="rpmH"
                     /locus_tag="PLB33_01540"
     CDS             complement(10424..10558)
                     /gene="rpmH"
                     /locus_tag="PLB33_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOG62233.1"
                     /translation="MKRTYQPKRKQRSSEHGFLKRMSTKSGRNVIKRRRARGRKKLSA
                     "
     gene            11015..12349
                     /gene="dnaA"
                     /locus_tag="PLB33_01545"
     CDS             11015..12349
                     /gene="dnaA"
                     /locus_tag="PLB33_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOG62234.1"
                     /translation="MNFEELWNEVLEIVKEDTNQVSFNTWFKPLKIVAYKNNTIYLET
                     ADDFLRNTIKKRHYNFLKNAFNYVLKKEIDIIFTIPGENIDKEESKKYNTNSLDDTDA
                     IYNGRILNPKYRFDNFIIGNSNRFAHAASLAVAEAPSTAYNPLFIYGGVGLGKTHLMH
                     AIGHYILDNNPDTYVLYVTSEKFTNDLINSIKDGTNEEFRNTYRKADVLLVDDIQFIA
                     GKESTQEEFFHTFNALHEANKQIIVSSDNPPSEIPTLEDRLRSRFEWGLIADIQPPDY
                     ETRIAILRKKAEAENYNIPDEVITYIAQHIQSNIRKLEGALIRIYAYASLTNKNEVSL
                     ELAQEALKHLISKNKKVSASDIKEVVANYFNISIDEIESKKKTKNIAYPRQIAMYIAR
                     KLTDLSLPKIGEEFGGRDHSTVLHAFNKVEEDMENSQEVKKNIEDLISMLNN"
     gene            12591..13700
                     /gene="dnaN"
                     /locus_tag="PLB33_01550"
     CDS             12591..13700
                     /gene="dnaN"
                     /locus_tag="PLB33_01550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOG62235.1"
                     /translation="MKLKINQNELNKAINIVQKAVSSRTPLPILSGILIEAKDNILTL
                     TATDLELGIKTYFPCEIEEEGSIVVQSRLIGDFVRKLPSNTYVFIETMGNNNMEIKCL
                     NSEINILGNSADEYPNNTFDNEGETFKIKSEVLKNLIKHTYFAAAIENIKPLFTGCLI
                     EIKNNLCTVVALDGYRMAVKKEKIDYKGDISIVIPSKTLFEILRIIEENKDETEITVS
                     ESHISFKVENTIIISNLLEGKFIDYEGIIKDNYVTVVKVNTEDMRDSIERASLLARDD
                     KNNLIIMDIKDFNMQINSASEYGNVEENISIDKEGEDIKIGFNSKYILDYLKVIDSEM
                     ISLNLIGKNNPCFIKEENEENKDNYIYMVLPVRIS"
     gene            13789..14001
                     /locus_tag="PLB33_01555"
     CDS             13789..14001
                     /locus_tag="PLB33_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG62236.1"
                     /translation="MEKININTEYIKLDQLLKYTNAVEGGGMAKNVILDGLVKVNGEV
                     VLQRGKKLREGDIIEFNNEKYLIERY"
     gene            14048..15163
                     /gene="recF"
                     /locus_tag="PLB33_01560"
     CDS             14048..15163
                     /gene="recF"
                     /locus_tag="PLB33_01560"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOG62237.1"
                     /translation="MIVKKLIIKNYRNFKEAEAELNNSLNIFIGDNGQGKTNLMEAIY
                     IASIGRTFRLNSENELINFFENKSSVEIYIEKNNYKIKIEILLEKNKKKQVIINGVKL
                     EKTSEMIGILNNVIFTPDDMKIIKGSPVERRRFINIDISQIKPKYKYLLNKYKKVTTE
                     RNLLLKNYYTKCENKDIISIWNDYLVNTGTEISLYRNEYINKLKKAATDIYSSISGNK
                     EKLDILYLSNIGCITDRDEVKKIFYDKINKNFNNEILKGISLYGPHKDDLIIKINGKE
                     CKYFGSQGQQRSVILALKLAEIEIIKEETGEYPILLLDDVLSELDNKRKSYLIEHIKD
                     IQTIITSTDEHELNILTKNFDKKMFYINEGKISNISN"
     gene            15175..17088
                     /gene="gyrB"
                     /locus_tag="PLB33_01565"
     CDS             15175..17088
                     /gene="gyrB"
                     /locus_tag="PLB33_01565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOG62238.1"
                     /translation="MATDKNNNHYSADQIQVLEGLEPVRKRPGMYIGSTGERGLHQLV
                     YEVVDNSIDEAMAGYCDTIKLTINEDNSVVVVDNGRGIPIETNKKTGKSTLETVLTVL
                     HAGGKFDNDAYKVSGGLHGVGVSCVNALSEYLVAEVNWDGKKYRQRYERGIAKTEVEF
                     IGNTNKTGTSITFAPDPTIFDTTVFNYTTIKHRMRELAFLNKGIKIILEDKRQKIKNE
                     FHYEGGIREFVQFLNSKKEPLHNDVIYTEGTKDKVNVEIAIQYTDSYNENIFSFVNNI
                     NTIEGGTHLVGFKTALTRVINDYAKKANLIKENEVGITGEDIREGITAILSIKITNPQ
                     FEGQTKTKLGNSETRGIVDSITGEALSIYCEENPKTAKAIVEKALKSARAREAARKAR
                     DLVRRKGALDSMALPGKLADCSEKDPALCEICIVEGNSAGGSAKEGRDRRTQAILPLR
                     GKILNVEKSRIDRILNSEEIKNMITAFGCGIGEDFDISKLRYHKIIIMTDADVDGAHI
                     RTLLLTFFYRYMKELITGGYVYAAQPPLYRVRKGKFEKYVYSDDELNQVLDEIGRDNK
                     NEIQRYKGLGEMNPEQLWETTMNPENRILLRINEDDAIEADEIFNVLMGDKVAPRREF
                     IEENAKYVQNLDI"
     gene            17104..19542
                     /gene="gyrA"
                     /locus_tag="PLB33_01570"
     CDS             17104..19542
                     /gene="gyrA"
                     /locus_tag="PLB33_01570"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOG62239.1"
                     /translation="MSENVNKNKIVNINISEEMKRSYLDYAMTVIVSRALPDVRDGLK
                     PVHRRILYSASELGLSPDKPHKKSARIVGDVLGKYHPHGDTAVYDAMVRLAQSFSMRY
                     PLIDGHGNFGSIDGDEAAAMRYTEARMSKIAMEMLRDFNKDTVDFRPNFDETLKEPVV
                     LPSRFPNLLVNGSSGIAVGMASSIPPHNIGEVINGIIAYIENPDIDIPGLMRYIKGPD
                     FPTGAIIEGKDNIKNAYLTGRGILKVKAKAEIEELKNNKSQILVSELPYQVNKARLIE
                     KIADLVKDRIIEGISDIRDESDRDGMRIVIEIKRDYNPNIVLNQLFKHTQMQDSFSVI
                     MIALVNNEPKVLNLKEMIYHYVEHQREVIVRRTKYDLDKAEARAHIVEGLLIAIDNID
                     EVIKIIRASYDDAEKRLMEAFNLTEIQARAIVDMRLRRLQGLEREKLNVEYEDLIKMI
                     NKFKEILSNQNLIDQIIKDELKEIKNSFYDERKTKITADEGEINVEDLIEEENVAITL
                     TNFGYIKRLPEDTYKVQNRGGKGITALTTRDEDFVKDLFITSTHDYLMFVTNKGKIYK
                     IKAYEVPEARRQAKGTAAINLINIEPGEKIKSLIPIREFNDNEFLIFVTKKGIIKKTK
                     LSEFESNRKTGLVAIKIAEDDELISVNKTDGTKDIFIVTKQGKGIRFHESDVRETGRN
                     TMGVKAITLSKNDELVAMSIIRKSDENKHILTVTENGYAKITLSQEYRPQSRGGKGMK
                     AHNINKKTGLIVNSLIVDKEDDIMILSTSGTIIRMHARDISIMGRDTQGVIAMRLKGD
                     DKVASIAKIFSEED"
     gene            19571..19882
                     /locus_tag="PLB33_01575"
     CDS             19571..19882
                     /locus_tag="PLB33_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOG62240.1"
                     /translation="MSLEIESKIQDSIIEIKPIGEVDIYTSPELKNQIFSLIEERNTS
                     IIIDGESLEYIDSTGLGVLMSIYKKMQENSLNLEIINLKPNIYKLFDITGLNKVFNIR
                     E"
     gene            19887..20261
                     /locus_tag="PLB33_01580"
     CDS             19887..20261
                     /locus_tag="PLB33_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62241.1"
                     /translation="MDKILLKIPKKSEYMSTIRLTSSSLFNLNGFNIDEIEDIKVIIS
                     EICTFFIMNLSRSTEDFEISYQVFKNKIIVTVADLNDGELSEESLNNDMSILIIESLS
                     DNFNYDLNNKTITFEKNKIGVD"
     gene            20263..21024
                     /locus_tag="PLB33_01585"
     CDS             20263..21024
                     /locus_tag="PLB33_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HOG62242.1"
                     /translation="MIEPSKNSKRIKNYDLFLLYHETKDIEIRNQIFEKYKYMAEIIS
                     RKYSNRGIEHDDIYQIACMGLIFAIERFDISKGFEFTSFATPTILGEIKKYFRDKGWA
                     VKVPRKIQEISRKVNEYNNILTARLHRAPTIKEIAEYISSTEEEVLEAFEAGRMFNSQ
                     SLDEKFDLSRDDNDISLMDVVGAEDKHYNLIENLDFLNKSMSKLNELEKNIIIKRFYE
                     NKTQSDIAKELKISQMTVSRIEKKSLEKLRIEYYR"
     gene            21036..23867
                     /locus_tag="PLB33_01590"
     CDS             21036..23867
                     /locus_tag="PLB33_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOG62243.1"
                     /translation="MRNKGRLFVLGIVVIVFLIINSFTNIVEFITNYLWFKEVGYTQT
                     FFTKIKAQIMIGIPIFIILSILLYIFIKKLKKKYDDETEIIVVNKKFNLTIKLISAGA
                     SLLITLNIISTMWFEILQYINGVEFGATDPIFNNDVSFYVFKLPLINTIVSSIISILF
                     LLTVIIILFNGFLALKDSIKNVSEKFEDIQQFPRKQIDLNNILNKKFVERIINQLSII
                     GVFLFLLLALRYVLRSYDLLYSRLGRVFGAGYTDINVTLNLYRILAVGCVLAAITFFI
                     GARKRNLKTALAVPAALIIISIAGTGVAGAVEKFVVEPDQLSKETKYMQYSIKSTQNA
                     YSLNNVKTIQFPANNNLTIEDLQNNPEVIDNIRINDQEPLIQVYNQLQGIRPYYVFND
                     VDVDRYVIDGDYKQVFLSARELDQDRLNEQARTWVNQYLKYTHGFGITVSTVNNVTPQ
                     GQPEILVKNIPPTTETDFNIQRPEIYFGEKTNNYVIVNTDEMEFDYPSGADNVETIYE
                     GKAGINLSFFNRLMFSIRKGSYRMMISNNIDSNSKILINRNIMQRVTEIAPFMYYDPD
                     AYIVVNQDDGKLYWIIEGFTVSNRFPYSQPTDIFIKGMSVNYIRNSVKVVIDAYDGTI
                     DFYVADENDPIIKTYDKIFTDLLKPIDEMPEGIRKHIRYSRAFFDVQSDMYRLYHIEN
                     VTVFFGREDYWDLANEKYMGGGEVPAGSSYLMFKLPGEENVEFLLTNQYTPQNKDNMI
                     ALLAARNDGENYGELVLYEFPKTKVIPGPNMIETKIDQDTNISSQLTLWSQLGSDVLR
                     GNTLVIPIEESLLYVEPIYLKSDTDSNFPEMKMVVVSYGDKILMEPTLDTAINRLFGI
                     TEQEPGRPQVPDEEYDDTNINDLIIKANEVFNDANEASQNGNWAEYGRKINELERLLN
                     QLNALINGQQEQEARDENVQEESSGMPSE"
     gene            24037..24762
                     /locus_tag="PLB33_01595"
     CDS             24037..24762
                     /locus_tag="PLB33_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOG62244.1"
                     /translation="MGSAGLSTNVSIILVFAEGFLSFFSPCVLPLIPVYISYLAGNGR
                     KVNSDGTITFERKKVLFNTLFFILGISSVFFILGLSFSTLGIFFNNNRELFSKIGGII
                     IVIMGFVQLNIIKLNFLKVEKRINLNIGGKINPILAYIMGFTFSFAWTPCVGPALASV
                     LILASNATNAFAGNMLVFVYALGFIIPFIILGVFTTEALNFFKEKRQLVKYSIKIGGI
                     ILIIIGIMTFTGIFTAISRNLSF"
     gene            24913..25788
                     /locus_tag="PLB33_01600"
     CDS             24913..25788
                     /locus_tag="PLB33_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOG62245.1"
                     /translation="MGDIVDIIIIGAGPAGLSAAVNAVTRGKTVRIFSNKENNLSKAE
                     RVDNHLGFYHASGKELMDKFKEHAKAMDIKIEEGMAVNILPLGESFMVNVNGEIFESK
                     KVILAMGITKVKELPGEGRLLGNGVSYCATCDGMLYRGKNALVWDQSKDAWEEANFLQ
                     SIGVNVTFISKKPKQENLSNDIKFIEGTITEIIGENTVEAVNTGNDLIKTDAVFMLRD
                     AVAPAALIDGLELDGNFINTNKHMETNIPGLYAAGDITGKPLQLSKAIGEGLIAAQHA
                     ALQIDKKENLNGRKY"
     gene            25772..26101
                     /gene="trxA"
                     /locus_tag="PLB33_01605"
     CDS             25772..26101
                     /gene="trxA"
                     /locus_tag="PLB33_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOG62246.1"
                     /translation="MAENIKIINSTQEFDDMIKQNRYVLAEFWAEWCTPCRMVAPVIE
                     KVAEQYSGKINVAKVDIDEQQELAIRYGIQTIPTIIFFKEGKFASKEIGVKPLASFAR
                     MIDSNIA"
     gene            complement(26280..26813)
                     /locus_tag="PLB33_01610"
     CDS             complement(26280..26813)
                     /locus_tag="PLB33_01610"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOG62247.1"
                     /translation="MINLSEIKKEDMDSIYKWFADTEFLKYYDYYPPNPLDKSDVDKM
                     FKYYEESGKSKVFAVRKESTIIGVAGFDDIIKENQVATLFIGLGSENERGKGYGTETM
                     RLLLKYGFENLNFHRIQLNVLEFNDKAIALYEKCGFKKEGIFREFVLRDGKRYNLLLY
                     GLLKSEWEKTEILRLRS"
     gene            complement(26823..27500)
                     /locus_tag="PLB33_01615"
     CDS             complement(26823..27500)
                     /locus_tag="PLB33_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOG62248.1"
                     /translation="MIDILTALFFILLTIIVFKFSLLVNDKFKIVFLNPILLTAFIII
                     VILKIFNFTYSDYYKGGKILVSALGPIVVVLAIPLYKNRHDLVKNFIPIVGGIISGII
                     TTFVSITVLCKLFGIDEIIFLSLLSKSVTTPMAVESTKMLGGNESVTILAVIITGLIG
                     AAFAPIVINKGKIKNNIAKGIGIGSAAHALGTSKAIEMNEEAGAASGLAIGLTGLLTI
                     IAVIIFA"
     gene            complement(27493..27870)
                     /locus_tag="PLB33_01620"
     CDS             complement(27493..27870)
                     /locus_tag="PLB33_01620"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOG62249.1"
                     /translation="MKKIFKIVIQIAIIYAIYQAGNFISSLISNIIVIPGNIIGMVIL
                     LVLLITNVLKFSIIEETSNFMLKYMSFFFVPITVGIMESYKLIQDSIVQIIIILFISC
                     ILVMYVSCKVTDVLISYREKSHD"
     gene            28096..29352
                     /locus_tag="PLB33_01625"
     CDS             28096..29352
                     /locus_tag="PLB33_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOG62250.1"
                     /translation="MDFENNFNDNLEKENQNNITNDSMSNEYGEINFVMNEPLIKKPK
                     KRRIKSVMGYIALVLVAAIIGGLTGSYVTANMLNAAAEKKAAESSINAQEVKINLTDD
                     VYFAVAVAEKSQKTVVGITTEVERQINTFWGPQKRTEQHMGSGFIVDSNGYIVTNSHV
                     IGDGNYKNITVSLIDGSIETGNVLWYDTNLDLAIVKINKTRLPAAELGNSDELMVGEP
                     AVAIGNPMTLDLERTVTQGIISGLNRSIQFEDGTVIEPLIQTDASINSGNSGGPLFNA
                     KGQVIGINTAKMTSAEGLGFAIPINIIKPVLTQVVTGGTFIPVYVGITGMDIETYEML
                     YGVDLMADYGVIIIETMKDSPAREAGLQPNDVIIEIDGKKTEYMVDIQKKLYEYQDGD
                     KSEFKILRNGEELKLVLTLRNRPDGI"
     gene            complement(29490..30149)
                     /locus_tag="PLB33_01630"
     CDS             complement(29490..30149)
                     /locus_tag="PLB33_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOG62251.1"
                     /translation="MDAKNVIAKRVAELLHDGDVVNLGIGLPTMVANYIPEGMDITFH
                     SENGFLGLGPCPKEGEEDWELVNAGGMPSSIVPGGMFFDSATSFSIIRGGHVDATVLG
                     AMEVDEKGNLANWKIPGKMVPGMGGAMDLVVGAKTVIIAMVHTQKGKPKILKQCTLPL
                     TAKEQVDIIVTEMALIRVTKDGLVLEELGPEATIEDVVAATEANLIISPNLKRFGVEE
                     E"
     gene            complement(30149..30802)
                     /locus_tag="PLB33_01635"
     CDS             complement(30149..30802)
                     /locus_tag="PLB33_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOG62252.1"
                     /translation="MNKIISINEAANMVKEGMTIMVGGFLANGTPETLIDALVEKNVK
                     NLTLICNDTGFPDKGVGKMVVNKQFKKMIVSHVGTNPETANQMNSGETEVVLTPQGTL
                     AEKVRCGGNGLGGFYTPTGIGTEVAEGKEIRVINGKEYMFETPLRADVALLRASVADK
                     KGNMIFNKTMKNFNPIMATAADLVIVEADKIVEVGEIDPEHVMCSGIFVDYIVGGDK"
     gene            complement(30870..32048)
                     /locus_tag="PLB33_01640"
     CDS             complement(30870..32048)
                     /locus_tag="PLB33_01640"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOG62253.1"
                     /translation="MKKAVIVSAARTPIGSFGGAFASLSAVDLGVIAAKEAIKRAGIE
                     PSMVEEVYLGNILGAGLGQNVARQVSLGAGIPIETPALTINMVCGSGLRAVSMAAQFV
                     ESGQCDIILCGGTESMTDTPYLLPKARWGHRMGDGKVVDYMVHDGLWDIYNDYHMGMT
                     AENVAEQYGITREEQDEFAAQSQNRAEEAQKSGKFADEIVPVIIPQKKGDPVVIDTDE
                     YPRHGSTVEKMSKLKPAFKKDGTVTAGNASGINDGAAALIVMSKDKAGELGLKYLCEI
                     AGYGSAGVDPKVMGLGPVPATKKALANAGWTIDDLDLIEANEAFAAQALAVIRDLGLN
                     PEITNVNGGAIALGHPIGASGARILVTLLYEMEKRDAKRGLATLCIGGGMGTSLLVQR
                     "
     gene            32341..34020
                     /locus_tag="PLB33_01645"
     CDS             32341..34020
                     /locus_tag="PLB33_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG62254.1"
                     /translation="MSSIKNSLDLGFIITDDKFNVLYCNEYFNTNIIKTDILNKKITM
                     FFQDINVDMDKNLYSVKCKDNSVYYVFHNKFKIYNHELYSFFFYDFTIGSELQKQIER
                     LNSQQYIYNEMLNKLQDGIYITDERGTTIYANDAFLNLSGLTREQIIGNTVYDLRKSG
                     ILPNSCCAKVIETLGPVSTINHYYKGQRCLVSGSPIFDENGNLSKTIAVIRDVTELDM
                     LMKTIAKEKNLFIKSDYNVKTEAARDPDEIINNVKMKNIYEKAKKISKVDSTVLILGE
                     TGVGKDFIATYIHSISNRSKGKFIKINCGAVPEHLLESEFFGYEEGAFTGALKGGKKG
                     LFEEANGGILYLDEIGDMPYTLQVKLLSAINDKMFYRIGGISPIEFNARIIAATNHDL
                     KQLVEEKKFRADLYYRLNVVTLIIPPLRNRREDIIPLSTQFLEYYNNKYGKNCYITSS
                     CLQSFLIYEWPGNIRELKNIIERLVLMAEGAEIGTELFRDNLILPDSIELTELSELFI
                     TGSKTLKEKMDEYEKEIIESTLAVSKNMKEASMRLGIDISTLVRKKQKYNL"
     gene            34169..34369
                     /locus_tag="PLB33_01650"
     CDS             34169..34369
                     /locus_tag="PLB33_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62255.1"
                     /translation="MFFINDLKRIINNDVIIVFLIISYILIFVTSKELKRNNYHRDYK
                     IVRFTGIIYGLLAIAAATAIYW"
     gene            34378..35883
                     /locus_tag="PLB33_01655"
     CDS             34378..35883
                     /locus_tag="PLB33_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOG62256.1"
                     /translation="MKDIDLTQKIDLDLNKNEQILRKIFENTDDIIFRKLEGGKIKKL
                     KMLLFYVDGMTSKEAISEYAISSLLEGLDLEKIQNMSNVQLQDAIVRTSISISEIQIL
                     TTIEECVDKILSGETVLLLENCNKGIMLSSRGWPMRGVQEPSAETLIRGARDGFNETM
                     KVNISLIRRRIRDPKLKVKYTQVGNRSKTDIAILYIEDLVNNTVLETVEKRINNIEID
                     AVLESSYIEEMIEDDGYSPFPQIENTERPDAAASALLEGRVVIAIDNTPSVLIAPAIF
                     VSFMQSSEDYYERWLPACATRILRYFALPIVMLLPALYVAVTQYHPNLLPTQLALYVA
                     ASRANVPFPPYFEALLMELVIELVREASFRITTPVGSTIGLVGGLVIGQSSVEAGLIT
                     PLAVIVVALTAIASFTIPSYNFGATLRLIRFGFIILAATFGLFGISIGLSFLIIHLCT
                     LKSCGIPYMTPFTRFVENKRDLKDTIVRPRISKMVRKPDYLHMEKKERKNV"
     gene            35876..36952
                     /locus_tag="PLB33_01660"
     CDS             35876..36952
                     /locus_tag="PLB33_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOG62257.1"
                     /translation="MFNQDITPYQNLSILLLVSFVLQSMLMPVILTGVDGPEGWISIL
                     IAAFILYFSIKTINKAMKKHDEDTIISVSDKLFSKFISKIIGLYYISMFITVNSLIIK
                     DFAEQIKLMMLFRTPISTIIITILLTASYATKKGIQTIANITNIALLTSLIPYLLIIV
                     FSTYYADYTNIFPVYPADAEGIIKSVPNAMLSFLGFSVLLFSNSRVDTKEKNMRINKR
                     YLVISAFLYIFSYILIIVKFGTKEASNLVWPFVSIMKFVNIPGFFFESTEIVGLCLQI
                     IVIFTSICILAFFTNLAMQETFKTNENGYFIFIQIPILYLMAAGLPGMYMMFPYIQVP
                     IYIISGMNLFIPMLVAYAGNRKKT"
     gene            37045..38142
                     /locus_tag="PLB33_01665"
     CDS             37045..38142
                     /locus_tag="PLB33_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOG62258.1"
                     /translation="MKKLILSLMIISTVFLFGCWDSVEINKREYLFAVGIDKEDDSLK
                     FISEIPKINEGSEEDRLVYTNEGKSFSDFYHLNYLHSDKVISDRLMQVIVLGEELARD
                     SDALKEIFDEIQRSPQMNRRVKICIAKGRAEDIINTEIPSNPIVGRYLSDMLVKLKRE
                     SYQDIFTFDEAILHLGQKGNAMIPVVEINEKSLKIERAAIVKEYELIGFLEPDELEAV
                     MLLLNPANANIRNINIEIDDTNISLGAVDVSMTDNIKLQGEKLQVDYYITLYCYIDSF
                     TIGNNNLEDRNFLNRVKDESIKKISETSTNAVDKLKNTYNADLLQIKDKLYKYHKRDY
                     EKIMDKYDEVFAKADINVYYKMEIKSVGLVK"
     gene            38151..38828
                     /gene="spoIIR"
                     /locus_tag="PLB33_01670"
     CDS             38151..38828
                     /gene="spoIIR"
                     /locus_tag="PLB33_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOG62259.1"
                     /translation="MKYSKKVFISAVFVVLAIAIAYSAQVYAGLSEINGKVIRLHVLA
                     NSDSFEDQELKYKVRNKIITTFNEEFEDINYKSESSNIIVEKLNQIRCEAEEIVREEG
                     YDYDVNVYYGNYKFPRKAYEEIVLPEGYYDAVRIEIGKAEGSNWWCVMFPPLCFVDFG
                     RNQDDPILDFETEEKLQQVLTEEEIEVIKTKRGIENIKLKSKIFEFIEKGKVNKTPNT
                     YAASLSN"
     gene            complement(38950..>39456)
                     /locus_tag="PLB33_01675"
     CDS             complement(38950..>39456)
                     /locus_tag="PLB33_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG62260.1"
                     /translation="NTVVETQQIRQDLFFRLSVITLVIPDLCKRIEDIPLLVEHFINK
                     YNKKFNKFFSGVSKEVLHIFLNYSWPGNVRELEHAIESAISLYDGEIIREEHLPFQFK
                     NYQPKNDNSWETGSIQPLSTAVEKVEREIIISALEQTDFNITKAAKLINVPRQTLQYK
                     IKKLGLLF"
BASE COUNT        13496 a         6497 c         6677 g        12786 t
ORIGIN      
        1 ccggctccgc ctcttccttt taaccctgaa tccttgacta atttaataac ttcatcggga
       61 gtcatttccg taagaacctt gccaagtgcc atataaccgt cccttgctat acactcgtta
      121 atattctcgg gatttattag tccgcagtta cgaagtgcaa ctctcttttg ttttttataa
      181 aagttcactt cacttaaagc ttttaatttt tcttccttta cactttcatc atataaatat
      241 tttgtgacta ttcttccttt taataaatgc tcttcaacta tttcatcaac tctgtcaaca
      301 gtcattcgag cataaaatga accttcagga tatataatta caaccggacc cgcttcacaa
      361 agaccaaagc agccggtacc aataacctgt acttcattat ctaaatttac ttcttttatt
      421 ttatcaataa atctttgtct aatttgagca gaattagaag aactacagcc cgttccaccg
      481 caaaccaaca catgagatct gaaaattttc ataaccatcc tcactttcta ccgttaattt
      541 tctgagccta ttgtatattc aactacaact ctgttattag ccaaatgttc ggatacaatt
      601 cttttagctt tttcagagtc aacatgaata taagttacct tttccttacc tggagcatag
      661 atttcaacta ttggctcata agtgcacatt cctatgcaac cggtctgtac aaccgacaca
      721 ttgttcaaat ttcgttttgc tacttcttct acaaatgtgg ctaaaacagg tcttgctccg
      781 gcagatattc cgcaggttgc catgcccacc acaacacgca catccttatc ggtatttcgc
      841 atttcaatat tcttaattga ttcatctctt aactttttta attcttccaa tgatttcata
      901 ataaccccct acttatattt tgccagaatt tcaggtattt ttttcggctc taattttccg
      961 tagacgtctt ggtctatcat cataacagga gcaaggccgc agcaacccaa acaacgagta
     1021 gcttcaaaag taaataaatt atcctgtgtt gttccaccta ccttaacatt taattctttt
     1081 gtcagtttgt ccataatcaa ctgtgcgcct cttacatagc atgctgtacc gagacatact
     1141 ccgattttgt gcttcccttt aggttcaatg gcaaattgtg tataaaatgt agcaactcca
     1201 tacacttcag ctaacggaac atctaattcg gaagaaataa atttttgaac ttctatcggc
     1261 aaatatccga aagtatcctg agctttatgc aatacctgca ttaaggatcc gtccacatcc
     1321 ttaactgaat caatgtactc cttaagctca ataaaaagct tttcatttgc ctttacatcc
     1381 agtgcttttg tcaaaataat aacctcctca ttgcattctt gatatttata aatattatat
     1441 tatttcgtta attaattgtc aattgatttc aaaaacatta acttaaaaga atgcaaatct
     1501 aatgaaatga ttaccataat taatcaaatt atgacttttt tcgcgttatt tttactaaaa
     1561 gaaattatat cttatataca attataaata tgtatattcg atataaatat taataaaaaa
     1621 tcataggtct aataattaac aattgttggg aaaggttgga aacacatagt tatgatgaga
     1681 tgatgacgtg atgggtcgaa atgtataaat agtgtcaaag catctcagag gtcaactcaa
     1741 tcttctatct ttagtaaatc cagtattctg tttaaatcgt cactgctcgt gtattctatt
     1801 tcaattttcc ccttatcttt tttgtttttg atattaaccc ttgttgaaaa tctttctgtc
     1861 agcaattctt ccaaatatac tatatattta tcttttacgg gcttttctct acgtcgcggc
     1921 tttttcgaag agcgcactaa cttttctaca tctcgtacgc ttaaattgtc tcgtattata
     1981 atatccgtca aagatttttg tttttctttg tccaagctta gtatagctct gccgtgaccc
     2041 gcagaaatta aatttttaat aattttgtcc ttcacatatt catctaaatt aataagtctc
     2101 attatattag tcacatatac tctggacttt cctactatgt ttgacacctg ttcctgagta
     2161 attccgtatc tatccataat aaattgataa gcatttgcct catctatcgg atttaaatct
     2221 tccctttgaa tattttcaat caatgctaat tcagaggcat ttttattttc tatatccctt
     2281 actatagaag gtattgtttt tatattggca tttttgcaag ctctccatct tctttctcct
     2341 gcaattatag tgtagagctc accatctttt cttaaaatta taggctgaat aacaccatat
     2401 ttttttatgg attccgtaag ttcatctaac ttatcctgtt caaaaagctt gcgaggctga
     2461 tttgggtttg gggaaatcaa atcaatatct atttcatgga ttttttcata atcttccttt
     2521 tctaaatctt cgggtattaa tgccgataag cctttgccta atgcttttct ctttgcagcc
     2581 atactatttt ctctcctcaa ttatttttaa taaattcttc agataatttt atatatgcaa
     2641 tgcttccttt tgatttatcg tcatatagca ttataggctg tccaaagctt ggtgcttcgg
     2701 caagccttat acttctcggt attacggttc tatatacttt atccttaaaa tattttttga
     2761 cttcctcaac aacttgtatc gaaagattcg tcctgccgtc aaacatattc aaaataacgc
     2821 cttcgatttc aagcttagga tttaaatttt tctttacaag cttaacagta tcaataagct
     2881 gtcctacacc ttccaaagaa tagtattcgc attgtattgg tataagtacg gtattggatg
     2941 cagtaagagc atttaatgac aatagcccca aggagggcgg acaatccacc ataataaaat
     3001 catatttgtt ttcaattaaa tttatttttt cccttaatgt tttttccctg aatttagtat
     3061 tggtaagctc aatttcagca cctgcaagct gaatatttgc gggagtaata tccaggtttt
     3121 ctatttccgt cttgaaaata attttgttga tgtcgtaatc ttcaatcaat acattgtaaa
     3181 ttgtatattc cagtttattt ttgttaaatc cgaatccgct ggttgtgttt ccctgagggt
     3241 ctatatcaat actaagtact ttctttcctt ttaacgccaa tgcagtacat aaattaacat
     3301 tggttgtcgt cttccccaca ccgccttttt gattgaaaat acttatgact ttggtcatat
     3361 catcaccctc tacatgcttt ttttaggaat tttcaccttt atttcaatgt aatcatctga
     3421 ttcgttctgc tcaaatttag catcaatacc tgtttttact atttcattgt aagcgttttt
     3481 tattgtattt atatatattt tataatttat aaaattcctt acataacgct tgtccgcttt
     3541 cttcttaata ttcagctcat cagcaattga atctacaatt ttttcggttt cacttacatt
     3601 taaatccttt ttaattactt tttcaacaat attaagaaga atttcttcat catttatctt
     3661 taacaaggct cttccgtggc gctccgttaa atttccttct cttaattttt ctttaaccga
     3721 ctccggcagt tttagaattc ttactttgtt tgcaattgct gattgagatt tgcccaattt
     3781 ttgagccaac tgtgtctggc ttattccaaa ttcgtttata agtctttcat aggcatatgc
     3841 ttcttccaga taatgcaaat cttcacgctg caaattttca atcaaagaca ttgcagcgga
     3901 ttcttcttcc ttggcttcaa taatcacggc gggaattgta tccatattta acattttaac
     3961 agctttatac cgcctttccc cggctactat ttcataaata tcatcataaa tttttcttac
     4021 cgtaataggc tgtataattc cataattatc tatggactga ctcaattcct tcaatgcctt
     4081 ttcatcaaaa gtttttcttg gctgattttt gtttggaatt attttattga catcgatatg
     4141 aacaatattg ctaatcattt cctccccctt attttaacgg atccttggcc ggctttcctg
     4201 cttttctcgg atacttgatt tctgttttgt ctgttttctc tattataaca atttttctta
     4261 taatatcagt acccggaaga ttaatttttt taatttcttt aatttctccg ccaagtttct
     4321 taattgcatt gtcggaactt gaaatttcct gggaaatatt gtcgcttttg tatgctgcaa
     4381 aatatcctcc tacttttaca aaaggaatac aatattcgct caaaacatta agaggggcta
     4441 cagctcttga tacacaaata tcataacatt ctctatattc cttattttgg ccgtaatctt
     4501 cagctctgcc gtgaataagt ttcacatcct ttaaattcaa agcttttacc acttcggaca
     4561 aaaaatctat tctctttctc aaactgtcca aaagcgtaat tttataatta ttattaacaa
     4621 ttttaagcgg aagccccgga aacccgcctc cggtacctac gtcaattata gttttgtttt
     4681 catttatatt attttcattc aacaaaagaa cactatcaat aaaatgcttt atataaattt
     4741 ccccatcatt ttctatggaa gtaatattta tttttttatt ccattccttt aataaatctc
     4801 tgtaaataat gaaattattt tcaatatctt tgctgtaggg taatttaagc tctttaaaac
     4861 cctcttccaa tgtttttatc acaagctcac ctttattctt tattgtaagt cattaaatat
     4921 attaacaaaa catttatgtc ggcaggcgaa acaccagaaa ttcttgaagc ctgaccaatg
     4981 gagttgggcc tgattttgtt tagtttctgt cttgcttcaa ttctgagact ttttacatca
     5041 tcgtagttaa tatcttccgg aatttttttg ttttcaagct ttttaaattg ctctacctgg
     5101 gctatctgct tttgaatata tccttcatat tttataactg tttccacata atcctttatt
     5161 tcttttctca atacaggtct ctttggatcc aagaacaagg tgttgtcata attcagctct
     5221 gttcttctca ataaatcata aagtgttgtc ggaattttta aagcagcgct tcccatatcc
     5281 tccaaagctt tattagtatc agcctttggt gttattttaa gattttttaa tctattgatt
     5341 tcattttcaa ttctttgtct tttatctttg aatcttttat atctttcttc tgttactaat
     5401 cctattttgt agcctttttc cgtaagtctt tcatcagcat tgtcctgtct tagaagaagc
     5461 ctgtactctg ctctcgatgt catcattctg taaggctctg ttgttccctt ggtaaccaaa
     5521 tcatcaatca gcactcctat atatgcttcc gagcggtcta aaataaaagg ctctttattg
     5581 tttaagctta aatatgcatt tattccggct atcaaacctt gtgccgcagc ttcttcatat
     5641 cccgagcttc cgtttatctg acctgcgaaa aataagttct taatacttat taattcaaga
     5701 ctcggcttga gctgggtagg gtcgatggca tcatattcta tagcgtaagc aggtctcata
     5761 atttcagcat tttcaagtcc tatcattgtt cgatacattt gaatctgaac ctcgtaaggc
     5821 agagaagttg acattccctg tatatacatt tcttttgtat caagaccttc aggctccaca
     5881 aaaagttgat gtttttcctt gtctgaaaat ttaactacct tatcttccaa tgatgggcag
     5941 taccttggtc ccacactttt tataacaccg ccgtaaagcg gcgacctgtc taaattattc
     6001 cttattaatt catgagtctg ttgggtagtg taggtaatat agcacggaac ctgctcaatg
     6061 tttatttcat cattcataaa tgagaaaggt attatttcat catcaccggt ctgtattgtc
     6121 attttttcat aattaatgga atccttgtgt attcttgcag gtgttcccgt tttaaacttt
     6181 ataagctcaa ttcctaagtc catcaatgaa tcggacaatt catttgcagg agccaaacca
     6241 tcaggtcccg atgaatagtt caattcaccc atataaattc ttccctttaa atacgtgcct
     6301 gtagtaacta taaccgctct tgccctgtag tatgctccaa gcttggtata tacattaaag
     6361 ctcttatctt catttacttt tatcttagtt gcttctgttt gtattaccct taaatttttc
     6421 tgattttcta ttaccttttt catttcccta tgatactttt ttttgtcggc ttgggctctt
     6481 aaggaatgaa ccgcaggtcc ttttgagcgg ttcaacatct tacattgtat catagtttta
     6541 tctatattta aagccatttc tccgccaaga gcatcaattt ccctgaccaa gtgtcctttt
     6601 gccgtccccc cgatattggg attgcatggc atcatcgcta ttgaatcaag gcttaaagtc
     6661 agcattattg tttttaaacc aagacgtgca gtcgccaagg atgcttcaca tcctgcatga
     6721 cctgcaccaa ttactaacac atcaatgctc tcctcgagaa attctcttac tctgtcttcc
     6781 attgttgttc ctttcctatt ttcctatgca aaattcatcg aatattttgt caattaaatc
     6841 atcgctgacc gatttaccta caatcaatcc caaattttcc attgcttgca ttaaatctat
     6901 ctcgataaaa tcaatgggca tattgttttc aattgatttt aacacttctt caatgttgtt
     6961 ttttgctttt atcaataaat tcttatgtct tacgttgtta ataataagct catcccttat
     7021 atctaaccgg cctttaaaaa ataaagagta aattttatta atgatatcat ccagcccttg
     7081 attttgtaaa atagatacat tgataatctc ttcttctatt tttttataag aagacaaatc
     7141 cagcctgctt tccaaatcag ttttattcct tatatatata acctttttat ctttaatata
     7201 atctaatata ctttcatcct ctttttcaag ctcttttgat gaatcaaaaa tcattattac
     7261 aaggtcggct tcctttacct tttctaaagc tttttcaaca cctattcttt ctaccatatc
     7321 atcggtttct cttatacctg cagtatcgat tattcttaac ggcacgccct taatattcac
     7381 gtattcctct atagtatccc ttgttgttcc cggtacttcg gtaacaattg cacgattttc
     7441 attcaaaaga aaattcataa gagacgattt tcctacattc ggttttccaa gtattactgt
     7501 ttttatacct tccttgatta tttttccggt atcagccgta tttataagtt tatccaactc
     7561 aggtactaag ttatgacata aagctttaac tttttcaata gttatttcat cctcgtcata
     7621 ttcgggaaaa tcaatattaa cttcaatgtt ggccaacaat tccattattt cgtctataac
     7681 tgcattaatt tgttttgaaa gtctgccttc cagatgtttt attgaaattt catgacttga
     7741 ttcggtcttg gaatttatta tgtcaattac tgcttctgcc tgagttaaat caattctgcc
     7801 gttaagaaaa gctctttttg taaattctcc tctttcagct gttctgcagc cataatgaag
     7861 gacagtatcc agtatttttt ttgcggaaat aatcccccca tggcagttga tttcaataat
     7921 atcttctctg gtatatgtgt taggagcctt catgtaagat aagagtactt catctataac
     7981 ctttttatct ttatccacaa taaacccgta atgaaggtat cttggcttta aattatccgg
     8041 tttccgttta tttttatcaa cgaaaatttt atatgcaata ttcaatgcat catttccgct
     8101 taatcttatt atattaattc ctgcattgcc gggaaatgtt gcaattgcag caatagtatc
     8161 gttaaacata tcttcatctt acctttccgt gtcttcatac cttataatat tttaccttaa
     8221 ttttatataa atgtacatat ttttctatct tgaattactg taaccaagaa aatataaaat
     8281 aacccttttt cagggttata agtggttgga attttgttat ttttggtcta ttactacttt
     8341 cctgtatggc tcttctcctt cggaataagt aataatacct tttacgtcct gtaatgttga
     8401 atgtattatt cttctttcat aaggattcat aggctcaagt tttaccttgc gattgtaata
     8461 tctgcatttg tctgccattt taagagctaa tctttttaaa gtttcttccc tctttgctct
     8521 gtaatcttca acattgaaaa ttactcgcac atagttttct cttcccttat ttaccattag
     8581 attcaagaga aactgaattt catctaaatt tttacctctt ttgccgataa caattccttt
     8641 atcgtcttct ccgattttta caatgtcaac atttaaaata ttgtcagaat aattaacctc
     8701 ataatcagct tctatgctca tttttttaag caaaatgtca ataaattttc ttgctctctc
     8761 ttcaggacca ttggtaatag taacttttac ctttgcatcc ttgctgccta aaccgaaaaa
     8821 accttttgaa ggctcattga ttatttcaat ttcaacctca ttattctcgg cgcggagttc
     8881 tttaagtgct gcggctatag cttcttcaac ggtaggtttc tcaattgtta catttctcat
     8941 ctattttaat tcctccttcg gatttttgga cgaatttaaa acaataaatt gttgcactaa
     9001 ctggaataga tttgatataa cccaatataa tgccaatcct gcaggaaatt gtatggaaaa
     9061 tacaaatatc attataggca tcatcatatt catggttttc tgagttgagg cttgctgttc
     9121 atttatggag ggttgcgccg tcatttttgt ggtaaggtat gttgtaatac ctgtaattac
     9181 agccaatacc ggaagtgcag atcccaaaaa aggaagagtc atgccgccca ttgacaatcc
     9241 gttaaccatg atatcttgag catttaatac ttccataact tgatttaaat gcatgcttga
     9301 acttatacta aactcttctt ttatagcatt tgtcaataca atccaatcgt tattctttaa
     9361 aaaccatact gtattttcat taacatttaa atctttaatc cataaaaaag ccctgttgat
     9421 ggaatcataa aatgcttgat ctccaaaaac gtattttacc ggttctcgaa ttacataaaa
     9481 aaatgcaata agtattggga attgaattaa taaaggcaaa catccggaaa acggactgta
     9541 atttgcttct ttatataatt ccatttgttt tctctgtaaa gtttgcggat cctttccgta
     9601 cttttgctgt aactcctgaa tttttggact tagttcctgc atttttttca tagatttact
     9661 ttgctttaat gttaaaggca atactattaa tttaaataat atggtggaaa ttataattgc
     9721 tatggaataa gcagataaat acttagagtc aacaacagaa accatattat atacaagttt
     9781 aacaagcatg cccataggca ctgctataaa atctaaattc atttttaccc tcctatttta
     9841 aaggatcata tcctccgggg ttaaacggat gacattttaa tatccttcgt atacctaaat
     9901 atgaaccttt taaaaaaccg tatttttgta gtgcttcaac aaaatattgc gagcaagtgg
     9961 gataaaacct gcaatgtctt actgcagact ttgaagaaaa cttctgatat attcgtataa
    10021 taaaaattaa tactttactc aaattgtgta cctatatatc ttttttcttt tcatcagttt
    10081 aatgaatgag ctttcaaggc ttttataatc ggagtcaacg gcattaatcc ttgccattag
    10141 tacaaaatca tagccttctt ctaaataatg ctcgtttgct cttactattt cctttagtct
    10201 tcttcttata acatttcgat gtactgcttt tttaattttt tttgctgtag taaagccgaa
    10261 tctgttatag ttgtaattat ttttttttat aaataatacg agattattat cagatattga
    10321 attattgcaa ttatatacag ctttgtaatc attattcttc ttaattatta tcatttcgtt
    10381 accttattgc gattacatgg aataaaaggc cacatatgcg gccttatgct gacagttttt
    10441 ttctacccct tgcccttctt ctctttatta cattccttcc ggattttgta ctcattctct
    10501 ttaaaaatcc atgctcactg cttctttgtt ttctcttcgg ctgataagtt cttttcattg
    10561 aaacacctcc ttgttaccaa tctatacata aattatcata tcagtagatt atattatcat
    10621 taaatacttg tgtcaacact gaaattatgg acattctcct gactgcaaag actcttgctc
    10681 gaggacattt cctcattccc gcagagagta gatggcgtct ccgttgacta ttgtaatata
    10741 ctatattttt aaatatattg caaatatttt ttaaaatatt taaaattgaa ttgaatttgt
    10801 ggataaaaaa tgatagtatg gtaagtgtct gtggataact ttttttatca acatttaaaa
    10861 tggacaactt attatcaacc attacaaata ttaaatattc aattactttt atgttcatat
    10921 ttttatacac aactgtttat acccataata ttatcaatat ataagatatt aatcaaccta
    10981 ttgttaattt tttttatcaa taccggaggc aattatgaat tttgaagaat tatggaatga
    11041 agtacttgaa atagtaaaag aagatactaa tcaagtaagt tttaacacct ggtttaaacc
    11101 tcttaaaata gtagcatata aaaacaatac aatttatttg gaaactgccg acgatttttt
    11161 aagaaataca atcaaaaaaa ggcattataa ttttcttaaa aatgcattta actatgtcct
    11221 aaaaaaagaa attgatatta tatttactat tccgggagaa aacattgata aggaagaaag
    11281 caagaaatat aacacaaata gtttagacga tacggatgca atatataacg gaagaatatt
    11341 aaatccaaaa tacagatttg ataactttat aataggaaac agcaatcgtt tcgctcatgc
    11401 tgcttcctta gctgttgcgg aagcaccttc aacagcatac aatcccttgt ttatatatgg
    11461 gggcgtaggt cttggaaaaa ctcatttaat gcatgcaatt ggacattaca tattagataa
    11521 taatcccgac acttatgtac tgtatgtaac cagcgagaag tttacaaatg atctaattaa
    11581 ttcaattaaa gacggcacca atgaagaatt tagaaataca tacagaaaag ccgatgtatt
    11641 attggtagac gatattcagt ttattgcagg aaaagaaagt acccaggaag aattcttcca
    11701 cacctttaat gctttacatg aagcaaataa acaaattatt gtatcgagcg ataatccgcc
    11761 ttctgaaata cccactttgg aagacaggct gcgctcacgt tttgaatggg gtttaattgc
    11821 agatattcag ccgccggatt atgaaacgag aattgcaata ttaagaaaaa aggctgaagc
    11881 agaaaattat aatattcctg atgaagtaat tacatatatc gcccagcata tacaatcaaa
    11941 tattcgtaag cttgaaggag cactaattcg tatttatgct tatgcttcct taacgaacaa
    12001 aaacgaagta tctcttgaat tagctcagga agcattaaag cacctgatat caaaaaataa
    12061 aaaagtatcg gcatctgata ttaaagaagt tgttgctaat tattttaata tttcgattga
    12121 tgaaatagaa tccaaaaaga aaaccaaaaa tattgcatat ccaaggcaaa ttgccatgta
    12181 tatagcaaga aaactgactg atttatctct gcctaaaata ggtgaagaat ttggaggcag
    12241 agaccattca acggttctcc acgccttcaa taaagttgaa gaagatatgg aaaacagtca
    12301 agaagtgaaa aaaaatatag aagatttaat ttcaatgctt aataattaac tttaaaatgt
    12361 tgataaactg tttgtaatta tttatgaaaa cataaagatt ttatatgtct tggataaata
    12421 ataaaacaat caacagtata aattttttat aatcttgaaa atacaaactt ttatatagtt
    12481 ttcaacaaat taacaatacc tactaatatt acttattatt attatatttt tctactatgg
    12541 caagcaaaat tattttatca acaataaata ttattactaa ggagtctatt atgaaattaa
    12601 aaattaacca aaatgaactt aataaagcta tcaatattgt acaaaaggct gtatcttcaa
    12661 gaacccctct tcctattttg tcaggaatac ttattgaagc aaaggataat attctaactt
    12721 taacagcaac agatttagaa ttaggaataa aaacatattt cccctgtgaa attgaagaag
    12781 aaggttcaat tgtagtacag tcaagattaa taggagattt tgtcagaaaa cttccatcaa
    12841 atacatatgt gtttattgaa accatgggaa acaataatat ggaaataaaa tgcttaaatt
    12901 ctgaaattaa tatacttgga aattcagcag acgaatatcc taacaataca ttcgataatg
    12961 aaggagaaac ttttaagata aaatccgaag tacttaaaaa tttaataaaa catacatatt
    13021 ttgctgcagc aatagaaaac ataaaaccgt tatttacagg ttgtcttatt gaaataaaaa
    13081 ataatttgtg taccgttgta gctcttgacg ggtacaggat ggctgtaaaa aaggaaaaaa
    13141 tcgattataa gggagatatt tcaatagtta taccttctaa gacattgttt gaaatactga
    13201 ggattattga agaaaataaa gatgaaacag aaataacggt gtctgaaagt catatttcct
    13261 ttaaagttga aaatacaatt attatttcaa accttctgga aggaaaattc atagactatg
    13321 aaggaattat taaagacaat tatgtaactg ttgtaaaagt aaataccgaa gatatgagag
    13381 acagtattga aagagcttcg cttcttgcaa gagatgataa aaataatttg attattatgg
    13441 atattaaaga tttcaatatg cagataaatt ctgcctccga atacggcaat gttgaagaaa
    13501 atatatcaat tgataaagaa ggggaagata taaaaatagg ttttaattca aaatatattt
    13561 tagattattt gaaagtaata gacagtgaaa tgatttcatt gaatttaata ggaaaaaata
    13621 atccatgttt tataaaagaa gaaaatgaag aaaataagga taattatatt tacatggttc
    13681 tgccggtgag aatcagttaa caggtttcct gtttgtcatc ctgagcgtag cgagggattt
    13741 catgagattc tacaggctag ccttcagaat aattagaaag ggtattgtat ggaaaaaata
    13801 aacattaata cggaatatat taaattagac cagctgttaa aatatacaaa tgccgttgaa
    13861 gggggaggta tggctaaaaa tgtcatattg gacggattag tgaaggttaa cggagaggtt
    13921 gttctgcaaa gaggaaaaaa gttaagagaa ggagatataa ttgaattcaa taatgaaaag
    13981 tatttaattg agcgttatta ggtataggta cataccaagc aagttatggg gatactgatg
    14041 aggaagaatg atagtaaaaa aacttataat taaaaattac aggaatttta aagaagctga
    14101 agcagaatta aataattccc ttaatatttt tatcggagac aacggacaag gtaagactaa
    14161 tcttatggag gcaatttata ttgcttccat agggagaact ttcagattaa acagcgaaaa
    14221 tgaactgatt aacttttttg aaaacaaaag ttcagttgaa atttatattg aaaaaaataa
    14281 ttacaagatc aaaatagaaa tattattgga aaaaaataaa aagaaacaag ttattataaa
    14341 tggtgtaaag cttgaaaaaa catctgaaat gataggtatt ttaaataatg ttatttttac
    14401 gcccgacgat atgaaaatta taaagggaag tcctgttgaa agacgcagat tcattaatat
    14461 tgatatttca caaatcaagc ctaaatataa atatttgctt aacaaatata aaaaagttac
    14521 cactgaaaga aatctattgt taaaaaacta ctatacaaaa tgtgaaaata aggatattat
    14581 tagtatttgg aacgattacc tggtgaatac gggtacggaa atatcgcttt acagaaatga
    14641 atacataaat aaattaaaaa aagcagcaac ggatatatat tcaagtatat cgggaaacaa
    14701 ggaaaaattg gacatattat atttaagcaa cattggatgt attacggaca gagatgaagt
    14761 aaagaaaata ttttatgata aaatcaacaa aaattttaac aatgaaattt taaagggaat
    14821 atccctttac ggtcctcata aggacgattt aattataaaa atcaacggta aggaatgtaa
    14881 atactttggc tctcaagggc agcagcgctc tgtaatatta gcattaaagc ttgccgaaat
    14941 cgaaataata aaggaagaaa caggcgaata tcctatttta ttattggatg atgtattatc
    15001 tgaacttgac aataaaagaa agagctatct tattgagcat attaaagata tacaaacaat
    15061 aataacaagt actgatgagc acgagctaaa tattttaact aaaaatttcg ataaaaaaat
    15121 gttttatata aacgaaggaa aaattagtaa catttcaaat tagaggagaa ataaatggcg
    15181 acagataaaa ataacaatca ttacagcgca gatcaaattc aggttcttga aggtttggag
    15241 cctgtaagaa aaagacccgg aatgtatatc ggctctacgg gagaaagagg acttcaccag
    15301 ctggtttatg aagtagtaga caacagcata gatgaagcaa tggccgggta ttgcgatacc
    15361 attaaattaa caattaatga agataattca gttgtggttg tagacaatgg gagaggcatc
    15421 cctatcgaaa caaataaaaa gacaggcaaa tcaaccttgg aaactgtttt gacagtactt
    15481 catgcaggcg gtaaatttga caatgatgct tataaagtat cgggaggact tcatggagtt
    15541 ggtgtttcat gcgtaaatgc tctatctgaa tatttggttg ctgaagtaaa ttgggacggc
    15601 aaaaagtaca ggcaaaggta tgaaagaggt attgcaaaaa cagaggttga atttataggt
    15661 aatacaaata aaaccggaac aagtattaca tttgctcctg accctacaat ttttgataca
    15721 acagtattta attatacaac tattaagcat agaatgagag agttggcatt tttaaataag
    15781 ggaataaaaa taatccttga agataaaaga caaaaaatta agaatgagtt ccattatgaa
    15841 ggaggaatca gggagtttgt acaattttta aacagcaaga aggaacctct tcacaacgat
    15901 gttatttata ccgagggaac aaaggataaa gtaaatgtag agattgcaat tcaatatacc
    15961 gattcctata atgaaaatat attttcattt gtaaacaata ttaatacaat tgaaggggga
    16021 actcatttag taggttttaa aactgccctt acacgcgtta taaatgatta tgcaaaaaaa
    16081 gcaaatttaa taaaagaaaa tgaagtagga attaccggtg aagatataag agagggtatt
    16141 actgcaatat tgtcgattaa aataacaaat ccccaatttg aaggtcagac aaagaccaag
    16201 ctgggaaaca gcgaaacaag aggaatagtt gacagtatta cgggagaggc tttaagcata
    16261 tattgcgaag aaaatcctaa aacagctaaa gcaatagtag aaaaagcttt aaaatccgca
    16321 cgggcaagag aagctgcaag aaaagcaaga gacttggtaa gaagaaaggg cgctcttgat
    16381 agtatggcac taccgggtaa attggcagat tgctcggaga aggaccctgc tctttgtgaa
    16441 atttgtattg ttgaaggtaa ttcggcagga ggctctgcaa aagaaggaag ggacagaaga
    16501 actcaagcaa tacttccttt aagaggtaaa atattaaatg ttgaaaaatc aagaatagac
    16561 agaatactaa attccgaaga aataaaaaat atgattacgg cattcggctg tggaatagga
    16621 gaagattttg atatttcaaa attaagatat cataaaataa taattatgac cgatgccgac
    16681 gtagacggag cacatataag aactttgctg cttacatttt tctacagata tatgaaagag
    16741 ctaataacag gagggtatgt ttatgctgcc cagccgcctt tgtatagagt aagaaaagga
    16801 aaatttgaaa aatatgtata cagcgatgat gaacttaatc aggtattgga tgaaatcgga
    16861 agagacaata agaatgaaat acaaaggtat aagggtcttg gagaaatgaa tcccgaacag
    16921 ctttgggaaa caaccatgaa tcctgaaaac agaattctat taagaattaa tgaagatgat
    16981 gcaattgaag cagatgaaat attcaatgtt ttaatgggag ataaggttgc tcccagaaga
    17041 gaatttatcg aagaaaacgc aaagtacgta cagaatttag atatataagc tgagaggttg
    17101 taaatgagcg aaaatgtgaa taaaaacaaa attgtaaata taaatatttc tgaagaaatg
    17161 aaaaggtctt atttagacta tgcaatgacc gtaatagtat caagagcatt gcctgatgta
    17221 agggacgggc ttaaaccggt tcacagaaga atattatatt cggcaagtga attggggctt
    17281 tcgccggata aacctcacaa aaaaagtgcc cgaattgtag gagatgttct gggtaagtat
    17341 catcctcacg gagatactgc cgtttatgat gcaatggtaa ggttagccca aagtttctcc
    17401 atgcgctatc cgttaatcga cggacacgga aattttggct ccatagacgg tgacgaggca
    17461 gcggcaatgc gttataccga agctcgcatg tcaaagatag caatggagat gcttcgtgat
    17521 ttcaataaag atacggttga tttcagaccg aattttgacg aaactttgaa ggagcccgtt
    17581 gtgcttccaa gccgttttcc taacttgctg gtaaacggtt catcaggtat agcggtagga
    17641 atggcaagct ccatacctcc tcataatatc ggagaggtaa taaacgggat aattgcttat
    17701 attgaaaatc ctgatataga tattcccgga cttatgagat atataaaagg tcctgatttt
    17761 cccacaggcg ccataattga aggcaaagac aacattaaaa atgcttactt gacaggaaga
    17821 ggaatattaa aggttaaggc taaagccgaa atagaagaat taaaaaacaa taaaagccaa
    17881 atattagtat cagaacttcc atatcaggtg aataaggcca gacttattga aaaaatagct
    17941 gatcttgtga aggatagaat aattgaagga atttccgaca ttagggatga aagcgacaga
    18001 gacggaatga ggattgttat tgaaattaaa agagattata atcccaacat agtacttaat
    18061 caattattca aacatactca gatgcaggac agcttcagcg taataatgat tgctttggta
    18121 aataatgagc caaaggtttt aaacttaaaa gaaatgatat accactatgt agaacatcaa
    18181 agggaagtta ttgtcagaag gacaaagtat gatttagata aagctgaggc ccgagcacat
    18241 attgttgaag gtttattgat tgcaattgat aatatagatg aagttattaa aattatcaga
    18301 gcatcctatg acgatgccga gaaaagatta atggaagcat ttaatttaac ggaaatacag
    18361 gcaagagcca tagtagatat gagacttaga cgtctgcaag gtcttgaaag agaaaaatta
    18421 aatgttgaat atgaagacct gataaaaatg ataaacaagt ttaaagaaat tctatctaat
    18481 cagaatttga ttgaccaaat aataaaggat gaattaaaag aaataaagaa tagtttttat
    18541 gatgaaagaa aaacaaaaat tacagcagat gaaggagaaa tcaatgtaga agatttaata
    18601 gaggaggaaa atgttgcaat aacacttaca aatttcggat atattaaaag gcttccggag
    18661 gatacttata aggttcaaaa cagaggcggt aagggaatta ccgcactaac cacaagagat
    18721 gaagactttg taaaggattt gtttataaca tcaactcatg attatttaat gtttgttaca
    18781 aacaaaggga aaatatataa aataaaggca tatgaagtac cggaagcaag acgtcaggct
    18841 aaaggaacag ctgcaataaa cttgattaac attgaaccgg gcgaaaaaat aaaatcattg
    18901 attccgataa gagaatttaa cgataatgaa tttttgattt ttgtaaccaa gaagggtatt
    18961 atcaaaaaaa ctaagctcag cgaatttgaa tcaaacagaa agacaggttt ggtggctata
    19021 aaaattgcag aagacgatga attgataagt gttaacaaaa cagacggaac aaaggatata
    19081 ttcattgtta cgaaacaagg aaagggtata agattccatg agagcgacgt aagagaaacc
    19141 ggcagaaaca ccatgggagt taaagcaata acactttcta aaaacgatga gcttgttgcc
    19201 atgagcatta ttaggaaaag cgatgaaaat aaacacattt taaccgtaac agaaaacggt
    19261 tatgcaaaaa taaccctgtc tcaggaatac agacctcaga gcaggggcgg aaaaggtatg
    19321 aaagcccaca acataaataa aaaaacaggg cttatagtca attcactaat agttgataaa
    19381 gaagatgaca ttatgatttt aagtacaagc ggaacgataa tcagaatgca tgcaagagat
    19441 atttccataa tgggaagaga tactcaaggt gttattgcaa tgagattgaa gggtgacgac
    19501 aaagttgcct caatagcaaa gattttttca gaggaagatt gaaaaaggaa gaataacgga
    19561 ggtaaaaaat atgtcattgg aaattgaaag caaaattcag gatagcatta tagaaataaa
    19621 accgatagga gaagtagaca tatacacttc tcctgagctg aaaaatcaaa tattttcact
    19681 gattgaagaa agaaatacca gcatcatcat tgacggtgaa agcttagagt atatcgatag
    19741 tacagggctt ggtgttttga tgagcattta taaaaaaatg caggaaaaca gtttaaatct
    19801 ggaaataatc aatttaaagc caaatattta taaacttttt gatataacag gtttaaacaa
    19861 agtgtttaat atccgggagt gatgaaatgg ataaaatatt gttgaaaatt ccaaaaaaat
    19921 ccgaatatat gagcacaata agattaacat catcgtcact atttaatcta aacggattca
    19981 atattgatga aattgaagat ataaaggtaa taatatctga aatttgcaca ttttttataa
    20041 tgaatttaag ccgcagcacg gaagattttg aaatatctta tcaagttttc aaaaataaga
    20101 ttatagtgac ggtagcggat ttaaatgatg gtgaattatc cgaagaaagt ttaaataatg
    20161 atatgagcat attgattatt gaaagtttgt ccgataattt caattatgat ttaaataata
    20221 aaacaataac ttttgaaaaa aataaaatag gcgttgatta atatgattga accgagtaaa
    20281 aactccaagc gtattaaaaa ttatgatttg tttttacttt atcatgaaac caaagatata
    20341 gaaataagaa atcaaatttt tgaaaaatat aaatatatgg ctgaaattat atccagaaaa
    20401 tacagcaaca gaggtatcga gcatgacgat atatatcaaa ttgcatgcat ggggcttatt
    20461 tttgccatag aaagatttga tatatcaaaa ggatttgaat ttacaagttt tgcaacacca
    20521 accatattgg gagaaataaa aaaatatttt cgagataagg gttgggctgt taaggtgccc
    20581 agaaaaattc aggaaatatc aagaaaagtc aatgaataca acaatatttt gacagcccgg
    20641 cttcacaggg caccaacaat aaaagaaatt gccgaatata taagcagtac agaggaagaa
    20701 gtattggaag catttgaggc aggaagaatg tttaattctc aatcccttga tgaaaaattt
    20761 gatttaagca gggatgataa tgacatatcg cttatggatg ttgtgggagc tgaggataaa
    20821 cattacaatc taatagaaaa tttggatttt ttgaacaaat cgatgagtaa attgaatgaa
    20881 cttgaaaaaa atatcattat aaagaggttt tatgaaaata aaactcaaag tgatattgcc
    20941 aaagaattaa aaatatctca aatgactgtt tccagaatag aaaagaaatc tttggaaaaa
    21001 ttaagaattg aatactacag ataaaaggag gtaatatgag gaataaagga agattatttg
    21061 tactgggaat tgttgtcatc gtttttttga ttatcaattc ctttaccaat atagtagaat
    21121 ttattacaaa ttatttgtgg tttaaagaag tcggctatac ccaaacattt tttaccaaaa
    21181 ttaaagcgca aattatgatt ggtattccga tttttattat attgagcatc ttattgtaca
    21241 tatttattaa gaaactaaag aagaaatatg atgatgaaac agaaataatc gttgtaaata
    21301 aaaaatttaa tttaactatt aaattaattt ctgcaggagc aagtcttctt ataacattga
    21361 atataatatc caccatgtgg tttgaaattc ttcaatatat aaatggtgtg gaatttggag
    21421 caaccgaccc tatctttaat aatgatgtga gtttttacgt atttaaacta cctcttataa
    21481 atacaattgt aagttcgatt atttccatat tatttttgct gacagtaatt ataatattgt
    21541 ttaacggctt tcttgcactt aaggacagca taaaaaatgt ttcggaaaaa tttgaagata
    21601 tacagcaatt tccacgtaag cagatagact tgaataatat tttgaataaa aagtttgttg
    21661 aaagaataat aaatcaactt tcaataatag gtgttttttt atttttactg ttagccctga
    21721 gatatgtatt aagaagctac gatttgcttt attcgcgttt aggccgtgta ttcggcgcag
    21781 gatatactga tattaacgtt actttgaatc tatacagaat attagccgtt ggatgtgtac
    21841 ttgcagctat aacatttttt atcggcgcaa gaaaaagaaa tttaaaaacg gcattggcag
    21901 ttcccgcagc tcttattatc atatcaattg ctggaaccgg cgtggcaggt gccgtagaaa
    21961 aatttgtagt tgagcctgac caattgtcaa aagaaacaaa atatatgcaa tacagcatca
    22021 aaagcaccca aaatgcttat tctttaaaca atgtaaaaac aatacagttt cctgccaata
    22081 ataatttaac cattgaagat ttacaaaaca acccggaagt aatagacaat atcagaataa
    22141 atgaccagga gccgttaatt caggtttata accagctgca aggtatcaga ccctactatg
    22201 tctttaatga tgtagatgtg gacagatatg taattgacgg cgattacaag caggtttttt
    22261 tgtcagcgag agaacttgac caagacagat taaatgaaca ggcaagaacc tgggtcaatc
    22321 aatatttaaa atatactcac ggatttggaa taacggtatc taccgtgaat aatgttacac
    22381 cacaggggca gcctgaaata ctggttaaaa atattcctcc cacaacggaa acagacttta
    22441 atatccagag acctgaaata tattttggcg aaaaaaccaa taattatgta attgtaaaca
    22501 cagacgaaat ggaatttgat tatccttcag gtgccgataa tgttgaaacc atatacgaag
    22561 gtaaagcagg aatcaacctt tcttttttca acagacttat gttcagcata agaaaaggca
    22621 gctacagaat gatgatttcc aacaatattg acagcaattc gaaaatttta ataaatagaa
    22681 atataatgca aagggtaact gaaatagctc cttttatgta ctatgacccg gatgcatata
    22741 ttgtagtgaa tcaagatgac ggtaaattgt attggattat tgaaggattt accgtaagca
    22801 acagattccc ttattcacaa cctacagata tatttattaa gggtatgagt gtcaattata
    22861 taagaaactc cgtaaaggtt gtaatagatg cttatgacgg cacaattgat ttttatgtgg
    22921 cagatgaaaa tgacccaatt ataaaaacct atgacaaaat ttttacggat ttattgaaac
    22981 cgatagatga aatgccggaa ggtattagaa agcatattag atattcacgt gcatttttcg
    23041 atgtacaatc agatatgtac agattgtacc acattgaaaa tgtaacggtt ttctttgggc
    23101 gagaggatta ttgggattta gcaaatgaga aatatatggg tggaggagaa gtacctgcag
    23161 gttcaagtta tctgatgttt aaacttccag gagaagaaaa tgtggagttt ttattgacca
    23221 atcaatatac tccccagaac aaagataata tgatagcatt gttggctgca cgaaatgacg
    23281 gcgaaaatta cggtgaactt gtgctgtacg aatttccaaa aacaaaagta attcccggac
    23341 ctaatatgat agaaacaaaa attgaccaag atacgaatat atcatcacag cttaccttgt
    23401 ggagtcaatt gggttcagat gttttgagag gaaacacgtt ggtaattcca atagaagaat
    23461 cattgctgta tgtagagccc atatatttga aatcagatac agacagcaat tttcccgaaa
    23521 tgaaaatggt tgttgtatcc tatggcgata aaatattgat ggagcctaca ctggatacag
    23581 ccattaacag acttttcggc ataactgagc aagaacccgg aagaccacag gtgcctgacg
    23641 aagaatatga tgatacaaat attaatgatt taattattaa agccaatgaa gtgtttaatg
    23701 atgcaaatga agcttcacaa aacggaaatt gggcagaata cggcagaaaa attaatgaat
    23761 tggaaaggct cttaaaccag cttaatgctt taataaacgg ccagcaagaa caagaagctc
    23821 gagatgagaa tgtacaggaa gaatcttcgg gcatgccctc agaatgatat gatacttcat
    23881 tactgactcg gttggttaaa tggatagaag taatatcaat aagataaata acagccacgg
    23941 gcaaggtccg tggctgttga aacatatgtg acaaggttac ttgacatagt aaagttgccc
    24001 tgctatattt taataatatt atttcgggag gtcaaaatgg gaagtgcagg tttatcaact
    24061 aatgtaagta taatattggt ttttgccgaa gggtttttat cttttttttc accatgcgtt
    24121 cttcctttaa ttcctgttta tatcagctac ttggcaggca atggaagaaa agtaaacagc
    24181 gatggtacta ttacatttga aagaaaaaag gtactattta acactttatt ttttatatta
    24241 ggtatttcca gcgtattttt tattttgggt ttatcatttt caactttggg tatattcttt
    24301 aataataaca gagagttatt tagtaaaatt ggcggcataa taatagtaat aatgggtttt
    24361 gttcaactta acataataaa gttaaatttt ttgaaagttg aaaaaaggat aaatctaaat
    24421 atcggtggga aaataaatcc aatacttgca tacataatgg gatttacatt cagttttgca
    24481 tggactccct gcgtaggtcc tgcactggct tcagtcttaa ttctggcatc aaatgccacc
    24541 aatgcatttg ccggaaacat gttggtattt gtttatgcct taggatttat aatacccttt
    24601 ataatattgg gagtatttac caccgaagca ttgaatttct ttaaagaaaa aagacaactg
    24661 gtcaaatatt caataaagat tggcggtatt attttaataa taataggaat tatgacattt
    24721 acgggaatat tcactgcaat aagcagaaac ctaagctttt agatgtccta accctgtttt
    24781 ttcgagccaa agcgaagaaa aaaacagcgg taggtcattc gcaacgaatt cccaatttat
    24841 gcgacaaagg agcaaataaa ttggtgaatg agactgcgtg agactgtaat acattgaaga
    24901 aaggaggcta atatgggaga tatagttgat attatcatta taggagccgg tcctgccggt
    24961 ttatccgcag cagtaaatgc tgttaccagg ggcaagactg tacgaatttt ttcaaataaa
    25021 gaaaataatc tgtcaaaagc cgagcgtgtt gataatcatt taggttttta tcatgccagc
    25081 ggaaaagaat tgatggataa gtttaaagaa catgcaaaag ctatggatat aaaaatagag
    25141 gaaggtatgg ccgtaaacat acttccatta ggtgaaagtt ttatggtcaa tgtaaacggt
    25201 gaaatttttg aatccaaaaa agtaatattg gcaatgggaa taacaaaagt caaagaattg
    25261 ccgggagaag gaagactctt aggcaacgga gtgagttact gcgctacatg tgacggaatg
    25321 ctttacagag gtaaaaatgc tcttgtatgg gaccaatcaa aagatgcatg ggaagaagca
    25381 aattttttgc aaagtatcgg agtgaatgta acctttatat caaaaaaacc aaaacaagaa
    25441 aatttaagca atgatataaa attcattgaa ggaaccatta ccgaaataat tggtgaaaac
    25501 actgttgaag ctgttaatac gggaaatgat cttataaaaa ccgatgccgt atttatgcta
    25561 agagatgcgg ttgcacctgc tgcattgatt gatggtttgg aacttgacgg taattttata
    25621 aataccaata aacacatgga aaccaatatt ccgggattat atgcggcagg agacattaca
    25681 ggaaaacccc ttcagctttc aaaagcaata ggtgaaggtt tgatagctgc tcagcatgca
    25741 gcactacaaa tagataaaaa ggagaattta aatggcagaa aatattaaaa ttatcaacag
    25801 tactcaggaa ttcgacgaca tgattaaaca aaacagatat gtattggcgg aattctgggc
    25861 agaatggtgt actccttgca gaatggttgc accggtaata gaaaaagtag ctgaacaata
    25921 tagcgggaaa attaatgttg caaaggtaga tattgatgag cagcaggaat tagctattcg
    25981 ctacggaata caaactatac cgactataat attttttaag gaaggaaagt ttgcctcaaa
    26041 agaaatcgga gtaaaaccct tagcttcttt tgcaagaatg attgacagca atattgcata
    26101 atatcatcct tagcatagcg aagattctca tgtatgagga cattcctctt acttcggaaa
    26161 ctatcctaag agtggaggtg tgtcctcata ccctgaaaat ctgagattct ttagtctatc
    26221 ccttataatt gtcacgagtt tatcttaccg cgataaagga atatggtctg tgattgtcat
    26281 cacgagcgta agcgaaggat ctcagttttt tcccattcac tttttaacaa gccatacaaa
    26341 agcaaattat atcttttacc gtctcttaat acaaattctc tgaaaatacc ttcttttttg
    26401 aaaccgcatt tttcatataa ggcaattgct ttatcattaa attccaatac attaagctga
    26461 attctgtgaa aatttaagtt ttcgaaacca tacttcaaca aaagcctcat agtttctgtg
    26521 ccgtatccct tgcctctttc attttcgctg cctaaaccga taaacaaagt tgcaacttga
    26581 ttttctttta ttatatcatc aaaaccggca actccaataa tagtgctttc ttttctaacg
    26641 gcaaacactt tcgattttcc gctttcttca taatatttaa acattttatc tacatcagac
    26701 ttatctaaag gattaggcgg gtaataatca taatatttta aaaactcagt gtcggcaaac
    26761 cacttgtata tactatccat atcttccttt ttaatttcag ataaattaat catatttgtc
    26821 tcctatgcaa aaattattac ggctattatg gtcaataacc cggttaagcc tattgcaaga
    26881 ccgcttgcag cgcctgcttc ctcattcatt tcaattgctt ttgaagtacc caatgcatgg
    26941 gctgccgagc cgattcctat tccttttgca atattattct ttattttgcc tttgtttatt
    27001 actataggtg caaaagcagc acctattaac ccggtaatta taactgctag aatagttaca
    27061 gattcatttc ctcccaacat cttagttgat tcaacagcca tgggagttgt aactgatttg
    27121 gacaagagtg ataaaaaaat tatttcatca attccaaaca atttacataa gactgttatc
    27181 gatacaaatg tagtgattat gccggatatt attcctccta caataggaat aaaatttttc
    27241 actaagtcat gtctgttttt ataaaggggt attgcaagca ctacaacaat aggtcctaat
    27301 gcagatacta atatttttcc acctttgtaa taatcagaat atgtaaaatt aaatattttt
    27361 aaaataacta ttataataaa ggcagttaaa agaatcggat tcaaaaatac tattttgaac
    27421 ttatcgttaa caagaaggct aaatttaaaa acaataattg ttagcaatat aaaaaaaaga
    27481 gctgttaata tgtcaatcat ggcttttctc cctgtatgaa atcagtacat ccgtaacctt
    27541 gcaggatacg tacataacta atatgcaaga tataaataaa attataatta tttggacaat
    27601 actatcttgt atcagtttgt acgattccat aattccaacc gttatgggaa caaagaaaaa
    27661 actcatgtat ttaagcataa aattagatgt ttcttcaatt attgaaaact ttaaaacgtt
    27721 ggttatcaat aataccaaaa gtatcaccat gcctataata ttgccgggga taactattat
    27781 attagatatg aggctgctta taaagtttcc tgcctggtat attgcataaa ttattgctat
    27841 ttgaatgact attttaaata tttttttcat ttttcctccg tatataacat tatatataaa
    27901 aactgacata attcaatatc tttttaattg cctgattttt aagaattgac ctgtgtgatg
    27961 ataatctgtc gcgggagtaa cagtaattct ttcgatttat tttatttatt aatttaagtt
    28021 tcaataaaga aaagaaggat ataattatac tatattgaat aaacgaaaat taaaataaat
    28081 aaggagcggt gtttaatgga cttcgaaaat aattttaacg ataatttaga aaaagaaaat
    28141 caaaataata taactaatga tagtatgtca aatgaatacg gtgaaattaa ttttgtcatg
    28201 aacgagcctt taattaagaa acccaaaaag cgaagaataa agtctgttat gggttacatt
    28261 gcccttgtat tagtagctgc aataatagga ggtctaacgg gcagttatgt aacagcaaat
    28321 atgttaaacg cggcagctga aaaaaaagca gctgagtcat caattaatgc tcaagaggtt
    28381 aaaattaatt taaccgatga tgtttatttt gcggtagcag ttgcagaaaa atcacaaaaa
    28441 acagttgtgg gaataacaac cgaggtggaa agacaaatta atacattttg gggtccccag
    28501 aaaaggacag agcagcatat gggctccggg tttattgtag attcgaacgg atacattgta
    28561 acaaattctc atgttatagg tgatggtaat tataaaaata ttacagtttc tttaattgac
    28621 ggttcaatag aaaccggtaa tgtattgtgg tatgacacca acttggattt agcaatcgta
    28681 aagataaata aaacaagact tcccgcagcc gaattaggaa attcggacga attaatggtt
    28741 ggggagcctg cggttgcaat cggtaatcca atgaccttag acctggaaag aacagttact
    28801 caaggtatta taagcggcct taacaggtct atccaatttg aagacggcac tgtcatagag
    28861 ccgcttattc agacagatgc ctccataaat tcaggcaaca gcggcggccc cttgtttaac
    28921 gccaaaggac aggtaatagg aatcaatacg gccaaaatga cctcagcaga aggattgggt
    28981 tttgctatac cgattaatat aattaaaccg gttttgacac aagtggttac aggaggaact
    29041 tttatacccg tatatgtggg catcacaggc atggacattg aaacttatga aatgctttac
    29101 ggagtcgatt taatggcaga ctatggagta attataatag aaacaatgaa agattctcct
    29161 gccagggaag ccggacttca gccaaatgat gttattattg aaattgacgg gaaaaagaca
    29221 gaatatatgg ttgatataca aaaaaaacta tacgaatatc aagatggaga taaatcggaa
    29281 tttaaaattt taagaaacgg tgaagaacta aaactcgttt taacattaag aaacagaccc
    29341 gacggcattt aaaaaaacag gagatcagct cctgtttcat attaataaca aatcccttta
    29401 tgtcattctg agcgcaagcg aaggctctca tcattattga aaagtgagaa tcttcaggct
    29461 gcgccttcag aatgacaatt tttattatat tattcttctt ccacgccaaa acgtttcaaa
    29521 ttcggtgaaa tgattaaatt tgcttctgtt gcagcaacaa catcttcaat tgttgcttcg
    29581 ggtcctaatt cttcaagaac caagccgtct tttgttaccc tgataagagc catttcggta
    29641 actatgatat caacctgctc cttagcagtc aaaggaagag tacattgttt taatattttc
    29701 ggtttcccct tttgagtgtg aaccattgca ataataactg tttttgcacc tacaaccagg
    29761 tccattgctc cgcccatgcc aggaaccatt ttacccggta ttttccagtt tgcaagattt
    29821 cctttttcat caacttccat tgctcccaag actgttgcat caacgtgtcc gcctctgata
    29881 atgctaaagg atgtggcgct gtcaaagaac attccgccgg gaactattga agaaggcatt
    29941 ccccctgcat ttacaagctc ccaatcttcc tctccttcct taggacacgg acctaatccc
    30001 aaaaatccgt tttctgaatg aaatgttatg tccattcctt ccggtatgta atttgcaacc
    30061 atagtaggaa gaccgatgcc taagttaaca acatcaccgt cgtgtaacaa ttcagcaact
    30121 ctttttgcta ttacgttctt tgcatccatt atttgtcacc tccaactata tagtctacaa
    30181 atatacccga acacataaca tgctccggat cgatttcccc aacttccact attttatcgg
    30241 cttcaacgat aactaagtct gctgctgtag ccattatcgg gttaaaattt ttcatagtct
    30301 tgttgaatat catattacct tttttatcag ctacggaagc tcttaataat gccacatctg
    30361 ctctcaaagg agtttcaaac atatattcct tgccgtttat tactcttatt tcttttcctt
    30421 ctgcaacttc agtcccgata cctgttggcg tgtaaaaacc accaagaccg tttccgccgc
    30481 atcggacttt ttcagcaagt gttccttggg gagtaagtac aacttccgtt tctcccgaat
    30541 tcatttgatt cgctgtttcg ggattagtcc ccacatgtga cacaatcatt tttttaaact
    30601 gtttattaac aaccattttt cccactcctt tatcgggaaa acctgtatcg ttgcaaataa
    30661 gggtaaggtt tttaacattt ttttctacca atgcatctat taaggtttca ggagttccgt
    30721 ttgccaaaaa tcccccaacc ataattgtca tgccttcctt aaccatattt gcagcttcat
    30781 ttatagaaat tattttattt atcaataata tcatcctttc tttttataat acaacaactt
    30841 gtcattgcta tgaatgacaa accgaattgt tatctttgaa ccaataaaga tgtgcccatt
    30901 ccgccgccaa tgcacaaggt agcaagacct cttttggcat ctcttttttc catttcatat
    30961 aaaagagtaa caagtattct tgctcctgat gctcctatcg ggtgccctaa tgctatagct
    31021 ccgccgttaa catttgttat ctcgggattt aatcctaaat ctctaattac tgcaagtgct
    31081 tgtgcagcaa aagcttcatt tgcttcaata agatccaggt catcaattgt ccatcctgca
    31141 tttgccaatg cttttttggt tgcaggaaca ggtcctaagc ccataacctt cgggtcaact
    31201 cctgctgagc cataacctgc tatttcacac aaatatttta atccaagctc acctgccttg
    31261 tccttgctca ttactattaa agctgcagca ccgtcgttta tgcccgatgc atttcctgct
    31321 gtaacggttc cgtccttttt gaatgcaggt ttcaattttg acattttttc aacggttgaa
    31381 ccatgccttg gatattcgtc cgtatcaatt actacagggt cgcctttttt ttgaggtata
    31441 ataacaggta ctatttcatc tgcaaatttt cctgattttt gagcttcctc tgctctgttt
    31501 tgactttgtg cagcaaattc atcctgttct tctcttgtaa tgccatactg ctctgcaaca
    31561 ttttcagctg tcattcccat gtgataatca ttgtaaatat cccatagtcc atcatgtacc
    31621 atgtaatcta ccaccttgcc gtcacccatt ctatgtcccc atctggcttt tggcaaaaga
    31681 tacggagtat cagtcatgct ttccgtaccg ccgcacaaaa ttatgtcgca ttggccgctt
    31741 tcaacaaact gtgccgccat acttactgct cttaagcccg agccgcatac catattaatt
    31801 gtcaatgcgg gtgtttcaat aggaatacct gctcctaagc tcacttgtct tgctacgttt
    31861 tgccctaatc ctgcccctaa tatattgcct aaataaactt cttcaaccat tgacggctct
    31921 ataccggctc ttttgatagc ttcttttgcc gcaattactc ctaaatcaac tgctgaaagt
    31981 gaagcaaaag caccgccgaa agacccgata ggtgttcttg ctgctgaaac aattactgct
    32041 tttttcataa tttcctccta aattttttta taatagcaat cctatgataa ttagtttgtc
    32101 tgccgtaata tgttgcattt tttatgccaa cattattatg taatttctgt tggagcttta
    32161 acattactgc tcaatttata tttcaaaaat atgcaagaat gcatatagac acaataaaat
    32221 tattgcataa ttgcataagt acatatataa taatagtggt acgatatact aagtacaaaa
    32281 tgaagaatgt gcttttcagg atatatataa taaagtaata acaatataag gaagaggatt
    32341 atgtcatcga ttaaaaatag tttagatttg ggttttatta taactgacga taaattcaat
    32401 gtattatatt gcaatgaata ttttaataca aatataataa aaaccgatat actaaacaaa
    32461 aaaataacaa tgttttttca ggatattaac gttgatatgg ataaaaatct ttattccgtt
    32521 aaatgcaaag ataattccgt atattatgtt ttccataaca aatttaaaat atacaaccat
    32581 gaattatata gttttttctt ttatgatttt actataggaa gcgagcttca aaaacaaatt
    32641 gaacggctca actctcagca atacatatat aacgaaatgc tcaacaaatt acaagatggc
    32701 atttatataa ccgatgaacg cggaacaacc atatatgcta atgatgcatt cttaaattta
    32761 tcaggactca caagagaaca aattataggc aataccgttt acgatttgag aaaaagcggc
    32821 atactgccaa attcatgctg cgcaaaggtt atagaaacct taggacccgt atcaaccata
    32881 aaccattact ataaagggca gcgctgcttg gtttcaggct cgcctatttt tgatgaaaac
    32941 ggcaatctgt caaagacaat tgctgttatc cgagatgtga ccgaattgga tatgttgatg
    33001 aaaactatag caaaggaaaa gaacttattt attaaaagtg attacaatgt taaaaccgaa
    33061 gcagcacgtg accctgacga aattataaat aacgtaaaaa tgaagaatat atacgaaaaa
    33121 gcaaaaaaaa tatcaaaggt tgacagtacg gttttaattt taggcgaaac aggtgtagga
    33181 aaggacttta tagccacata tatacacagc atcagcaaca gaagcaaggg taagttcatt
    33241 aaaattaact gcggtgccgt acctgagcat ttgctcgaat cagagttttt cggttatgaa
    33301 gaaggtgcct ttacgggtgc cttaaaagga gggaagaaag gactttttga agaagctaac
    33361 ggcggaatac tgtatttgga tgaaataggg gacatgccat atactttgca ggttaagctt
    33421 ttaagtgcaa taaatgataa aatgttttat cgcataggag gcatcagtcc tatagaattt
    33481 aatgcgcgta ttattgctgc aacaaatcat gatttgaaac agcttgtgga agagaaaaaa
    33541 ttcagagcag atttatacta tcgtttaaat gttgtgacat tgataattcc tcccttaagg
    33601 aacaggaggg aagacattat acccttatct acacaatttc ttgagtatta taataataaa
    33661 tacggtaaaa actgctatat tacttcaagc tgccttcaaa gttttttaat atacgagtgg
    33721 ccgggcaaca tcagggaatt aaaaaacata attgagcgct tggtattaat ggcagaggga
    33781 gcagaaatcg gaacggagtt gttcagagat aatttgattt tgcctgatag catagagctg
    33841 actgagcttt ctgagctatt tataacaggc tcaaaaacac tgaaagaaaa aatggatgaa
    33901 tatgaaaaag aaattattga atccacctta gctgtttcaa agaatatgaa agaagcatcg
    33961 atgagattag gtatagatat ttcaacattg gtcagaaaaa agcaaaaata caacctatag
    34021 atgacaaggg gacgggggta ctgtcataag gctcttttat taacataaag gagtcttttt
    34081 gttgtattta taatgttttt tcattttatg gtataaaaac ttaaaaaatg cagatattac
    34141 taacagctta tcccattaag gagggtccat gttttttatt aatgatttaa aaagaataat
    34201 aaacaacgat gtaataatag tctttttaat tataagctat atattaatat ttgtaacaag
    34261 caaggaatta aaaagaaaca attaccacag agactataaa atagtgcgtt ttacgggaat
    34321 aatatacgga cttttagcta ttgcagctgc cacagcaata tactggtagg ggagattatg
    34381 aaggatattg atttaactca aaaaatagat ttggatttaa ataaaaatga gcaaatttta
    34441 agaaagattt ttgagaatac cgatgacata atattcagaa aattagaagg gggcaagatt
    34501 aaaaagctta aaatgctttt gttttatgtg gatggaatga cttccaagga agcaataagc
    34561 gagtatgcaa taagctctct tcttgagggt cttgaccttg aaaaaattca aaatatgtca
    34621 aatgttcaat tgcaggatgc aattgtcaga acaagcattt caatttcgga aattcaaata
    34681 ctgacaacca ttgaggaatg tgttgataaa attctttccg gtgaaaccgt actattattg
    34741 gaaaactgca acaaggggat aatgctttcg tcgagaggct ggccgatgag aggtgtacag
    34801 gagccttccg cggaaaccct cataaggggt gcaagagacg gatttaatga aacaatgaag
    34861 gtgaacattt cattaatcag aagacgaata agagacccta agttaaaggt taaatatact
    34921 caggtaggaa atcgctcaaa aacagatata gctatattgt acatagaaga tttggtaaac
    34981 aatacagtat tggaaacagt tgaaaaaaga atcaacaata ttgaaataga tgccgttctt
    35041 gaaagttcat acattgaaga aatgatagaa gatgacggct attcgccttt tcctcaaatt
    35101 gaaaatactg aaagacctga tgctgctgca tctgcactat tggaaggaag agttgtaata
    35161 gcaatagata atactccttc cgtcttgatt gctcccgcaa tttttgtttc attcatgcag
    35221 tcttcggaag actattatga aaggtggctg ccggcctgtg caactcgaat attaaggtat
    35281 tttgccctgc ccattgttat gctgttgcct gctttgtatg tggctgttac ccaataccat
    35341 cccaacctcc ttcctactca gcttgctctg tatgtagcag cttcaagagc aaatgttccg
    35401 tttcctcctt actttgaagc cctattgatg gagcttgtaa ttgaactggt aagggaggca
    35461 tcctttagaa ttacaactcc tgtcggctca accataggtc tggtgggagg cttggttatt
    35521 ggtcaatctt cggtagaagc aggcttgatt acacctttgg ctgtaattgt tgttgcattg
    35581 actgcaatag cctcctttac aataccaagc tataattttg gtgcaacatt aaggcttata
    35641 agattcggat tcataatatt ggcagcaaca ttcgggttgt ttggaatatc cataggctta
    35701 agctttctca taatccatct ttgcacattg aaaagctgcg gaatacctta catgacacca
    35761 tttacaagat ttgttgaaaa taagagggac ttaaaggata cgattgtaag accaagaatt
    35821 agcaaaatgg tcagaaaacc ggattatctg catatggaga aaaaagagag gaaaaatgtt
    35881 taatcaagat attacacctt atcagaattt atcgatttta ctattggtat catttgtatt
    35941 gcagtctatg ttaatgcctg taatacttac gggagttgac ggtcccgaag gatggatttc
    36001 gatattaatt gcggcgttta tactttattt ttcaataaag acaattaata aagcaatgaa
    36061 aaaacatgat gaagatacga ttataagtgt ttcggataaa cttttttcta aatttatttc
    36121 taaaattatc ggactctatt acatatcgat gtttataacg gtcaatagtc tgataataaa
    36181 ggattttgcc gagcagatta aattgatgat gttgtttaga actcctataa gcactattat
    36241 tataacaatt ttattgactg cttcttatgc aacgaaaaaa ggtatacaaa ctattgccaa
    36301 tataacaaat attgctcttt taacatcgct tataccatat ttgctgataa tagttttttc
    36361 aacatattat gcagactata caaatatttt cccggtatat cctgcggatg ctgaaggaat
    36421 tattaaatca gtaccaaatg ccatgttgag ttttctcgga ttctctgtac ttttgttttc
    36481 taacagcagg gtagatacca aggaaaaaaa catgagaata aataagaggt atcttgttat
    36541 aagcgccttc ttgtacattt ttagctatat attgattatt gtaaagtttg gaacaaagga
    36601 agcttccaac cttgtttggc cttttgtttc tattatgaaa tttgtaaata ttccgggctt
    36661 cttctttgaa agtacggaaa ttgtgggttt atgcctacaa ataattgtta tttttacaag
    36721 catatgcata cttgcatttt ttacaaatct tgctatgcag gaaacattta aaacaaatga
    36781 aaatggttat ttcatattca ttcagatacc tattttgtac ttaatggctg caggactgcc
    36841 gggaatgtac atgatgtttc cgtatattca agtacctata tacataataa gcggtatgaa
    36901 tctttttata ccgatgctgg tggcatatgc aggtaataga aaaaagacat agttaggaat
    36961 aatcgtgtca ttattaatgc agtgaagaat ctcatgacaa cgggaaaatt ttttaacagt
    37021 atattgttta tataagaggt ctatatgaaa aaactaatac tgagtttaat gataatatca
    37081 actgtttttc tgttcggatg ctgggacagt gttgaaatca ataagagaga atatcttttt
    37141 gcagtcggta ttgataaaga ggatgatagt ttaaaattta tttcggaaat accaaaaata
    37201 aatgaaggaa gcgaagaaga taggctggtt tatacaaatg aaggcaaaag cttttcagat
    37261 ttttaccatc taaactacct tcattcggat aaagtaattt ccgacagact tatgcaggta
    37321 atagtgctgg gggaagagct ggcaagagat tcagatgcat taaaagaaat ttttgatgaa
    37381 atccaacgct ctccccaaat gaaccgcaga gtaaaaattt gtatagcaaa ggggagggct
    37441 gaagacataa tcaatactga aatacccagc aatcccattg ttggaagata tctgagcgat
    37501 atgcttgtta aattaaaaag agaaagttat caagatatat ttacttttga tgaggctatt
    37561 ctccatcttg ggcaaaaagg taatgcaatg atacctgtgg tagaaataaa tgaaaaaagt
    37621 ttgaaaatag aaagagcagc catagttaag gaatatgagt taatcggttt tttggaaccg
    37681 gacgaattgg aagcagtaat gcttctatta aacccggcaa atgccaatat caggaatata
    37741 aatattgaaa tagatgacac aaacatatct ttgggtgctg ttgatgtaag tatgacagac
    37801 aatataaaat tacaaggtga aaagcttcaa gtagattatt atattactct ttactgttac
    37861 attgattctt tcacaatcgg caacaacaac cttgaagaca ggaattttct gaatagggtg
    37921 aaagatgaat ccattaagaa aatttccgaa acttcaacaa atgctgtgga taagctcaaa
    37981 aatacataca atgcggattt actgcagatt aaggataaac tttataagta tcacaaaagg
    38041 gattatgaaa aaataatgga taaatatgat gaagtttttg caaaggcaga tataaacgtc
    38101 tattacaaaa tggaaataaa aagtgttgga ttagttaaat aggaggtact atgaaatatt
    38161 caaagaaagt ttttatttca gctgtattcg ttgttttggc aattgcaatc gcatattcgg
    38221 cacaagttta tgcaggcttg tccgaaataa acggtaaggt tatacggctg catgttttgg
    38281 caaattcaga cagctttgaa gaccaagagc tgaaatataa ggtgagaaac aaaataatta
    38341 ccacattcaa tgaagaattt gaagacataa actataagtc tgaaagttca aatattattg
    38401 ttgaaaagct taatcaaata aggtgtgaag cagaagaaat cgtaagagaa gaaggttatg
    38461 attatgatgt aaatgtatat tacggcaatt ataaatttcc tcgcaaagct tacgaagaaa
    38521 tagtcttgcc ggaggggtat tatgatgcag taaggattga aataggaaag gctgaaggca
    38581 gcaattggtg gtgtgttatg tttccgcccc tgtgctttgt agatttcggg agaaatcaag
    38641 atgaccctat attggatttt gaaactgaag aaaagctaca acaagtactt accgaagaag
    38701 aaatcgaagt tattaaaact aaaagaggaa ttgaaaatat aaaattaaaa tcaaaaatat
    38761 ttgaatttat tgaaaaaggc aaagtaaata aaaccccaaa cacttatgca gcttccctat
    38821 caaattagag acagtgggga cgtacctcgc tgtcccattc caatgttaaa gaggacggtt
    38881 attttagaat gattttttga cttaagtcca acagatacca gggacagcga ggtacgtccc
    38941 cactgtcacc taaaagagaa gccctaattt tttgatttta tattgcagcg tttgtcttgg
    39001 aacattgata agttttgcag cttttgtaat attaaagtct gtttgctcca atgcagaaat
    39061 gataatttcc ctctcaactt tttcaacggc agtacttaaa ggctgaattg agccggtttc
    39121 ccatgaatta tcgttcttcg gctgataatt tttaaattgg aagggaagat gttcctctct
    39181 tataatttca ccgtcataca gcgatatagc gctttcaatg gcatgctcaa gctctcttac
    39241 attgcccggc cagctgtaat ttaaaaatat gtgtaaaact tccttagaaa ctccactaaa
    39301 aaacttattg aatttcttat tatatttatt tatgaagtgc tctaccaata gaggtatatc
    39361 ttctattctt ttgcataaat caggaatcac caaggttata acactgagcc tgaaaaataa
    39421 atcctgtctt atttgttgtg tttctaccac tgtatt
//
LOCUS       DAOSHP010000008        39147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_799_length_39147_cov_9.451908, whole genome shotgun sequence.
ACCESSION   DAOSHP010000008 DAOSHP010000000
VERSION     DAOSHP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 39147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39147
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_799_length_39147_cov_9.451908"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLB33_01680"
     CDS             <1..479
                     /locus_tag="PLB33_01680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG62261.1"
                     /translation="FLEGSGFEVEMTRYDDSGLYTELSGTIRDKKNEDLKNRVELINN
                     SNADLVISIHLNSFPQKQYYGAHVFYQKSNEATTKLAADIIQESMKNILDKSNNRVPQ
                     IKKGIKIMDDAKVPVILIECGFLSNNSEERKLISEDYQEKTAWAIYTGLLKYLNEL"
     gene            643..1266
                     /locus_tag="PLB33_01685"
     CDS             643..1266
                     /locus_tag="PLB33_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62262.1"
                     /translation="MSFEEMEHFLNNIKSVLSSKVIIDSNNEIREIHVLADNSRHSKQ
                     IARDIRTALLSKFNEDVDYKIISVAQIDKNMSFNSDFRLLYEGYINETTPEKIKIKTK
                     LTWDGKEFIGEAEGFKSDKQSLIVAARSTLDAIRQAIEFDCFILEDIQYTKIAGEKAL
                     IVAINQINNGKENISVGASIIEDNQIDSAIKATLNAINRKICLLIKD"
     gene            1490..2803
                     /locus_tag="PLB33_01690"
     CDS             1490..2803
                     /locus_tag="PLB33_01690"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG62263.1"
                     /translation="MINNKQKKENNIKLYTYIFFLALTAIKLMHYLFNNYTISDYVLL
                     IVFSILTAIAETFLILLPKIGGVSVSFALTFSAILLTNPLTVSIISAIGMILRCPYVD
                     GKGRVHILNTPIYKTIFNVSQYIISFGVAGFVYEAIDKSFNFILIFFNPVAATATLVV
                     YILLNTFFMSMLMSILLKEKLVYIWKTNFFSLLVNVILVGLLGIVLAFAYYSYGSGGL
                     LLFFIPLLLARYTFKLYLDMRKNYFETLNVLVRAIEASDPYTSGHSMRVSAYAEAIAR
                     QVGLPQSKIDLIKSAALLHDIGKIGIDKNILNKTGKLKKEEFEKIKSHPEIGATIIAD
                     LSYLSNITDIIRHHHERNDGKGYPDGLCHSNISLETSILTIADSFDAMTTDRPYRSAL
                     SLESALQEIKNNEGTQFNPDIVDDAIIALKKAFFKIAEEKVEHVF"
     gene            2793..3377
                     /locus_tag="PLB33_01695"
     CDS             2793..3377
                     /locus_tag="PLB33_01695"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HOG62264.1"
                     /translation="MYFEIIFFSLLSIITAIVVLLIKKRDFDFDRYNIKIRGYKVLIL
                     AAVIKIAAEFLFNKYTNVTLLRILSHNWVIYFGLLYISLLNIKRSYMMLFFLGALFNF
                     AAIAANDFKMPVYVSELLSDVETKKMFLLSGNDLVHSLLTKETKLKFLCDIITLYPPY
                     PFPKTISVGDVFLLSGIFAFWQDLFYESSKILKH"
     gene            complement(3366..3881)
                     /locus_tag="PLB33_01700"
     CDS             complement(3366..3881)
                     /locus_tag="PLB33_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOG62265.1"
                     /translation="MNISDLFLPILIGLILFYGIIKGVDVYSAFVEGAIDGAKSALKI
                     FPYILAIIFAINLFIQSGAESFIVDKLTPITSFIGFPTELLSLSIIKPLSGGGSFGVF
                     QTILDNYGADSYIGRSASIIMGSSETIFYITAVYFGSVGISDYRYTIKAGLLSHAATI
                     FAALLVCKLMF"
     gene            complement(3878..4453)
                     /locus_tag="PLB33_01705"
     CDS             complement(3878..4453)
                     /locus_tag="PLB33_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOG62266.1"
                     /translation="MNKLWFGFVFCGLLFSFINKSPETVTNVLFFEIEKSVELILNLL
                     PIMAFWTGLMRIVEKTNILNKLSSFLKPLVRYLFKDVQNDANAVNAILMTLAANLFGI
                     GNSATAFGIKAMQEMQKSNLNKKTATKAMCMFLIINVSSIQLIPLNVIKLRADSGSVA
                     PSEIMVPTLLVTAFSTMVAIIFAKYYEGKEL"
     gene            complement(4559..5815)
                     /locus_tag="PLB33_01710"
     CDS             complement(4559..5815)
                     /locus_tag="PLB33_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG62267.1"
                     /translation="MDDDGISQVAILIIVLLISAYFSASETAFQKFNKSRMKNLASNN
                     KKAALVLKLDDDYDNLMSTIIIFKSIVNVTAASLVIFIFSKMFINNEILISTVLAIFF
                     IIILGELIPKGLANIAPEKAAMFSAPTMSIFLQICSPVNFLLAICKEMIYKGFKAEVQ
                     PSVYEDELRTMLDEVENEGVINKNESDLIRSAIEFNETVAEDIYTPRIDIVGVEENES
                     LESIKEKFILSGYSRLPVFSGDMDNIIGVLHEKDFYQGLNRKEKDIKKLISKTIFITP
                     NKKISELLKELQLSKVHMAVVIDEYGGTEGLITMEDIIEELVGEIWDEHDEVIEWFKK
                     IGDDKYLVSCNADIDDMFELLGVEIDEELDITTVNGFITMLFEGIPEEGGRIVYKDLD
                     ITVTKAEAKRVLEIQVERIKTEESLH"
     gene            6097..7416
                     /locus_tag="PLB33_01715"
     CDS             6097..7416
                     /locus_tag="PLB33_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62268.1"
                     /translation="MIFIMEMFIVTVENKWRYELLSFISIFNLIIFIIFTVFYSYQIF
                     YVFVALFNKSKNFNASINHKYAVVIAARNESAVIAQLINSIKKQNYPGELLDIYVVAD
                     NCTDNTAAVAKKAGAIVFERFNKQLVGKGYALDYAFKNILKSNKCYEGYFVFDADNLL
                     DENYVSEMNKVFDKGYKIITSYRNSKNYDTNWLSAGYSLWFLREAKYLNNSRMILNTG
                     CAISGTGFMVSDEIIRKNNGWKHHLLTEDIEFTIDSALQGEKIGYCGSAVLYDEQPYL
                     FKQSWNQRLRWAKGFYQVFAKYGIDLIKAIFKKRSFCCYDMFVTIMPALFLTLISVAV
                     NLTLFLIGLINIETYPELMNETIGAILLSVLNSYIVLFILGLITTVTEWKNINSSPLK
                     KIKYIFSFPIFIFTYIPISIVALFKFKKIEWKPITHSIVKTIEEVRQ"
     gene            complement(7555..7932)
                     /locus_tag="PLB33_01720"
     CDS             complement(7555..7932)
                     /locus_tag="PLB33_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOG62269.1"
                     /translation="MKNGISKELAAYLIAGILTTIVNFIVYYAMLFIGIDYKISNTAA
                     FIISVIFAFIINKKYVFLSDKSYFKEFIKFSLGRLFTYALDIGAMIILIEVFSVSEYM
                     AKLWTNILVMISNYLISKFWTFK"
     gene            complement(7932..8885)
                     /locus_tag="PLB33_01725"
     CDS             complement(7932..8885)
                     /locus_tag="PLB33_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017153413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62270.1"
                     /translation="MKKVSLLIPAYNEEETIPLLYNELNKVIDKISGYEFEILFVNDG
                     SSDNTLNILRDLRKRDSRVNYISFSRNFGKETAMAAGFDYVTGDATVILDADLQDPPE
                     LIEEMIFYWEKGYDDVYAKRRSREGETWLKKFTSAAFYKLLRKMTKIPIQEDTGDFRL
                     LDRRAIESLKKLREKQRYTKGMFSWIGFNKKEILFDRKPRAAGKTKWNYLKLFNLALE
                     GVTSFTTSPLRISTILGILVSIFSIVYMFIVLIKSLIWKDPVQGYPSMMVTILFLGGV
                     QLVCLGIIGEYVGRIFNETKYRPLYIVDEHNNEKIEDGGKL"
     gene            complement(8887..11112)
                     /locus_tag="PLB33_01730"
     CDS             complement(8887..11112)
                     /locus_tag="PLB33_01730"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOG62271.1"
                     /translation="MESIKRLGIFILIFAFSLVLLLKEPFIGIADNSDYYRVIQPLGF
                     KPEISNRYFYAYNFYTVNDMSSEDIKGSLSNIISPKVENDNEYFSTQFIFIKVSMIIN
                     YLLKIVLGKSPEIFNIKVLGILYAAIYSYGLCLFLTNINFKYKYINFLFLIIALVILC
                     DMGYLLYFNSFFGEAAIIASLMITLGLLTAIIKTESKIKSLFYVILFYIFALALTGAK
                     VANTPIGILIGIFSLALFIVKADWLSRAVILIGSILIICFSIFYYTNAPRWMSQVNNY
                     QSIFFGITKDSNEPEKDLEKLSIPLKYLPLTNTHGFLDHGEFDIYSDEFQKEVYDNAT
                     FLDILKFYFLNPSRAVEKLKLSADSSVIIRPSYLGNCSKEDEPERLSFTERFSLWSNI
                     RKNALGYAFYIIVSYSVLFFIINIYEIINNIKQYDYENTAFAFAALLLFLTTMSQFVL
                     PIIGNGEADLQKHMLLFNLCFDIMILVGICWLINNFYTKTVSAVVLTAFVVFCIAIFI
                     QTANEEIKETGTLKIGQYIYLGSYKNEPLKWVVLNKDENGYLLWCDNTVEYMEFDYSD
                     ETNSDNIYGSNNWIESDVRRWLFEFKSNFNDEEKLLIKDVKLKNILSYNNIEKSIGGN
                     KPFYWNSITSYVSQNYNTDAYYNYSAESVFLLDVYQLQKYVYENKISLKKQERYWLRT
                     PYYSSESMVRIVDKDGFVYHKDANVKAGVIPAVYIDENVSAIEGDGTYTSPIAIEKSR
                     R"
     gene            complement(11275..12342)
                     /gene="orr"
                     /locus_tag="PLB33_01735"
     CDS             complement(11275..12342)
                     /gene="orr"
                     /locus_tag="PLB33_01735"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HOG62272.1"
                     /translation="MYPRLVIDLKKVKNNLDRITEMVKGSGSSLMIVTKGYSADMEIF
                     KILDGSDIDYLADSRIQNLKKYEGAKKERVLLRLPMNSETDEVVKYADISLNSELKTI
                     KNLNESAKRQNKIHKILLMIDLGDLREGIFFKNEDEIYHTVEEILNLKNIELFGLGVN
                     LTCYGAVIPKSDNLSILVEIARKIEKKFDIKLQMISGGNSSSIYLIGRKELPEGINNL
                     RVGEAFLLGGETAYSQKLEGFYDDAFTLEAEIVELKEKQSVPIGETGVDAFGNKPVYE
                     DRGIIKRAIIAVGRQDVDPDALHPIDSKIDILGASSDHLILDISKSDMKYKVGDIVNF
                     KLSYSSLLRATTSGYVDRVYK"
     gene            complement(12351..13703)
                     /locus_tag="PLB33_01740"
     CDS             complement(12351..13703)
                     /locus_tag="PLB33_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HOG62273.1"
                     /translation="MKVNVLVAEIGSTTTVVNAFNGIGTGNPAFVGQGQAPTTVSEGD
                     VTIGLKGAIEDLKGNLQADTLTWDDMLATSSAAGGLKMTVHGLVYDMTVRAAKEAALG
                     AGGIIKMITSGRLRKTDLKKIQDIKPNIILVAGGVDYGERDTALYNMEMILSMGLKIP
                     IIYAGNIENQEEVRLMCEEADNQIYIVENVYPKIDTLVVEPTRKIIQDAFEEHIIHAP
                     GMAKVRDLVKGPIIPTPGAVMEAAKVLREEIGDLIIFDVGGATTDLHSVTEGSSEINS
                     ILISPEPVAKRTVEGDLGVYVNVSHIVEKIGMDNLKKEFPDAEELLENYKPIPKTDRE
                     KQFVERLTKEAVHTSLERHAGKLRYFYTASGKKTVAEGKDLTAIQYIIGTGGSLTRLP
                     DKKDILESVKTLAKEHELYPTEAARVLIDEDYILSSLGVLSKSYLSDALYLMKNSLRM
                     "
     gene            complement(13703..15898)
                     /gene="oraE"
                     /locus_tag="PLB33_01745"
     CDS             complement(13703..15898)
                     /gene="oraE"
                     /locus_tag="PLB33_01745"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HOG62274.1"
                     /translation="MKLEPNKKLDIKELLKDLDKYEPRRRGWHWREGRNEKRTVGDFE
                     YHETSECLKNSIPLPGSRGFGYIDPQPDCVITTEIASGRFEDDIRRMRMAAWHGADHL
                     MVIRTAGQSHIDSLLEGTNQGIGGIMVTRKQVRASRKACDLIEDEIGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKIMAWADMLQIDGA
                     HNANATAMKAWKVMPELMVQHAINTAFSVRVGIKPENIALSTVPPTAPPAPCMRLDLP
                     YAVALRDLFKDYKMRAQQNTKYMESDTREAAVTHTLNMVISRLTSADIQSTITPDEGR
                     NVPWHYNNIAGTMTAKQALTGMDGFREMVKLDREGPLGKEVRELKERAILFMEEILEV
                     GGYFKAVEGGFFIDSGKYPERNGDGIARQIDGGIGYGFIYERDEDYFAPVSVHYGYNN
                     IPAEFKKASDAIGGDTFEDRSKIQYIDELDEHDNVNVRLKEVEKYYDTNLVKPEVEWR
                     ADGIVLLTIFLPLDERTAEFAAIKCGEKMGLEDVTVVHKQCIHPSEGTYCEVKGRVNF
                     DIDINELVIPEKPDLLSEAEIREDIQNNPMFVVAATVGEDEHSVGLKETLDIKHGGIE
                     GFGIKYEYLGTSCPVEKLVDAAIETNADAILLSTIITHDDVHIKNMKKVHDLAIEKGI
                     RDKVMIICGGTQVTNEIALEAGLDAGFGRGTHGIDVASFLVKKRREKKA"
     gene            complement(15910..16260)
                     /locus_tag="PLB33_01750"
     CDS             complement(15910..16260)
                     /locus_tag="PLB33_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HOG62275.1"
                     /translation="MKRADDYQQRRAHLANLTDEELYERFWKLANEIVDPLLELGKKN
                     TTPSVERSILLRMGFSSLEVKPIVEGVMNKGLMGKGAGNVVWRLSKKLGCSVREAGLL
                     LAEGKHWDDVDTLF"
     gene            complement(16328..17728)
                     /gene="ortB"
                     /locus_tag="PLB33_01755"
     CDS             complement(16328..17728)
                     /gene="ortB"
                     /locus_tag="PLB33_01755"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HOG62276.1"
                     /translation="MSKDLSYAAVMARRPEIMKNSAGLDFSKFESGSIAFDYERMMKE
                     AGFTIEEIQKIQSEHGVGNTPIIELRNLTALARKIAPEGKGARIFIKDEAANASGSFK
                     ARRASTAVYQAKKLGYKGVVTATSGNYGAAVASQAAMYGLKCIVVQECYDSRGVGQPE
                     IIEKARKCEALGAEVVQLSVGPELFYTFLTILEETGYFNASLYTPFAVAGVETLGYEI
                     AMQFREKFGKDPDVVMATNAGGGNLTGTARGLKKAGAQSQIVAASVDLSGLHMASDTQ
                     FNRKSFTTGHTGFGMPFSTWPDRSDVPRSAARPLRYIDRYLIVHQGEVFYMTEALASL
                     EGLEKGPAGNTSLTAAFALAQELDQDQTIVVQETEYTGAGKHIQPQLSFARANGIEVK
                     FGDPKDEIPGKNIILPSHPSLIKANDADVIAMRKSLIRNALNNYKVTPTEADIEFLAA
                     ETNSSVEFVKETIASL"
     gene            complement(17721..18026)
                     /gene="ortA"
                     /locus_tag="PLB33_01760"
     CDS             complement(17721..18026)
                     /gene="ortA"
                     /locus_tag="PLB33_01760"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOG62277.1"
                     /translation="MVKKGEWVLIHRNVLEPAERVPHVPDDTKKVPLELWTKGYLQED
                     ANIGDEVTVLTRTKRLEKGTLLEVNPCYKHDFGNFVPELLKISEQVKEILFGGADNE"
     gene            complement(18110..19168)
                     /gene="ord"
                     /locus_tag="PLB33_01765"
     CDS             complement(18110..19168)
                     /gene="ord"
                     /locus_tag="PLB33_01765"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HOG62278.1"
                     /translation="MQNVKVIIWGLGAMGGGMARMLLEKKGVDIVGAVGRGAKLGKSM
                     YEIIGVERGDRPEVLVGTPEDVIKEKAADVVLCCTDSFTKVAFERLKFCLERKINVVS
                     SAEEMAFPQAQNPELAKQLDEIAKANGVSIIGTGINPGLIMDYLVVVMTGCCQTIDHI
                     TARRVNSLSPFGPAVMHEQGIGITVEEFKKGVETGTLSGHVGFHESIGMISEAIGWKV
                     DKVTTSMEPIVTDVDRKSPHGFAKAGDVAGCAMKGYGYVDGELKIEMDHPQQIEPEQV
                     GVQTGDYVIIKGTPNINLVNSPEVPGGIGTIAMCVNTIPMIINAKPGLHTMLTIPAPR
                     AIMGDMRDLIDPECKIVK"
     gene            complement(19414..20808)
                     /locus_tag="PLB33_01770"
     CDS             complement(19414..20808)
                     /locus_tag="PLB33_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG62279.1"
                     /translation="MKAKECLLLLQKILQLVDEGVHVLDKHGNTIIYNEAMSALEKMD
                     SADVIKKPFTEVFKGFNTENSTLLKALHSKTVTKNLKQTYLNKDGKEISTINTTIPVI
                     IDDEVVAAVEVAKNITKIEEMSNTILKLHDEIEKPEMVKHKKVKKYNFDNIIGHSENF
                     LDVIERAKKSSKNSASVFIYGETGTGKELIAQSIHYASARKDKPFKAQNCAALPESLL
                     EGLLFGTTKGGFTGAVDRPGLFEQANGGTLLLDEINSMPYELQPKLLRVLQENYIRRV
                     GGSTDIPIDVRIITTCNEPPEKILKEGKLRKDLFYRLNVIPINVPPLRERVDDIILLA
                     KMFVDKHNERFNKHVTGFSDEVYEYLLSYDYPGNARELENMIMSAVSMCEDDEYILTV
                     NHFSVHRSIEPRIDDYEKVEDSGIDEYLGNLEKRIIEKALLENNNNITRASNALKIKR
                     QTLQHKIKKYKISV"
     gene            21046..21525
                     /locus_tag="PLB33_01775"
     CDS             21046..21525
                     /locus_tag="PLB33_01775"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOG62280.1"
                     /translation="MKKIESFQVNHLKLKRGIYVSRKDKVGENIVTTFDIRMKEPNRE
                     PVINTAELHTIEHLGATFLRNHPVYDTRTIYFGPMGCRTGFYVIFSGNLTSLDVYEII
                     KEMFLFIMNYEGDIPGADEISCGNYQDMNLNMAKYEAAKFYNEVLLNIKDENLNYPE"
     gene            complement(21676..23499)
                     /gene="typA"
                     /locus_tag="PLB33_01780"
     CDS             complement(21676..23499)
                     /gene="typA"
                     /locus_tag="PLB33_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOG62281.1"
                     /translation="MKNKIINIAVIAHVDAGKSTLVDAFLQQSGVFRENQEVSEQIMD
                     SNDLERERGITIYSKNCSVMHNDIKINIVDTPGHADFSSEVERIIKTVDTVILLVDSI
                     EGPMPQTRFVLKKSLEIGIKPILLINKIDKKNHRAEEVVDMVFDLFVELNANDEQLDF
                     PILYGIAKQGIVQYELDEESHNINPLFDTIINHVQPYPDRDDEDLQLQVSTLAYDDYI
                     GRLGIGRVTAGTIKEGQIVSVAKHDGTVEKQKISNIYIYEGLKRTQVKEAFSGDIVIV
                     SGIANISIGDTICNENKIIPMETIKIEEPTMSMNFLINTSPFAGLSGKYVTTRHLKAR
                     LEKELEVNVGLRVEALEGALDGYKVSGRGELHLSILLENMRREGYEVAVSRPEVILKK
                     IDGIVMEPIERVIVNVPEIYSGSVISKLNLRKGIMVSMVPDGNYVKIEYLVPTRGLLG
                     YRTELINDSRGEGTMVRVFDHYEKFKGEIPQRINGVLISNDQGEAVAYSLSNLESRGI
                     LFINPGDKVYEGMIIGMNSRGEDITVNPCKTKKQSNIRSSAADDAIKLSPPKIMTLEA
                     ALEFINDDELVEITPDAIRLRKKYLTEIERRKYANKIKTSN"
     gene            complement(23734..24732)
                     /gene="trpS"
                     /locus_tag="PLB33_01785"
     CDS             complement(23734..24732)
                     /gene="trpS"
                     /locus_tag="PLB33_01785"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOG62282.1"
                     /translation="MENKNRIFSGMQPTGSLTLGNYLGALKNWVALQNEYDCYYCVVD
                     MHAITVPKEPKDLRKNTYEVLANYIAAGLNTEQVTLFIQSHVSAHAELTWILNCFSYY
                     GELGRMTQFKDKSKKQDSNAITAGLFTYPVLMAADILLYQADLVPVGEDQRQHLEITR
                     DIAQRFNNRFGETFVVPEPYISTDVAKIMSLQDPESKMSKSDSNENSYVLITDSPDVI
                     RRKFKRSVTDSIGVVNYTDEQPGVKNLINIYSKISNIKPEEIVSNYESKGYAKLKEDV
                     AEVVIEELKPIQERIDYLLKNKDYLEKIYNAGAQKAEVVANKTLKKAQKKVGFIPR"
     gene            complement(24907..25623)
                     /locus_tag="PLB33_01790"
     CDS             complement(24907..25623)
                     /locus_tag="PLB33_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62283.1"
                     /translation="MFDLKSIVIGLVLMIKKYSCLSLTIYEEANEEDYKNGIIALPII
                     GLAIGLVAFIIVSFRYIYDGFFISSAVLLYYCVVTKTTNIKDVYRTLNYYIKPANQTD
                     NISGIIGTVLICFMYLSLFRVVPVTSLIIMPAAGFSNLMILSKVIKRDLENTSILKYC
                     GNYHIIAAFGISFLSAAILNYKLIISLSLMFMLSGLIVSILDKRIKNVPSSVEGFIIE
                     VSQIMFLIITYVIRFGDTPL"
     gene            complement(25644..27005)
                     /locus_tag="PLB33_01795"
     CDS             complement(25644..27005)
                     /locus_tag="PLB33_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62284.1"
                     /translation="MIKIKKIICLVICISMLGTQAFGFSQAYNEFDIKKSIENTYGIN
                     IIIPEGVDYSEFIDCMSILERSIKKFPDNIIKEITDYYLSKGIATNVILDKTEIIKEL
                     FTLSIIDESSVNIYIKTLEGSLYSESHYASEQVITHVFSHFISDYMLETYDLDNLKDE
                     FDKLNEGFEYGTWSEEYSKVFVNRHSAISFENDIAHLIWYAEANPFILRNINGGDYAI
                     IHEKLRLIAEEFSKIFNSITENTKLWLDAIPQTPQDWAKDTITEMKNQSLIPDEFNGL
                     YEAYIKRGDFYKLVLNMLHIKLGENELNKYFNLTDYEEHVALDPLRGEVYVTDGLNYT
                     SYYNYLANSSNAFYEAYQMGILNTDSFYEPDEYMTRLEIAKLLAYIANELGMNISEFQ
                     TVSFNDIEQVAENEKPYIYIAADKGFIKGDGLNFKPFNYCTYQEAYLILNRFYNSLYD
                     YIQ"
     gene            27267..27746
                     /gene="rlmH"
                     /locus_tag="PLB33_01800"
     CDS             27267..27746
                     /gene="rlmH"
                     /locus_tag="PLB33_01800"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOG62285.1"
                     /translation="MKITIISVGKIKEKFFTEAIKEYIKRLSKYCKFIEEIVPDERAD
                     ESFSPAEIEQVKIKEGQKILNKIPKNSYVIVLDINGAQLSSEAFAEKLNTFGIEGISD
                     ITFIIGGSNGLWKEVIKSADFNLSFSKMTFPHQLFKVILLEQIYRAFKINSGETYHK"
     gene            complement(27772..29424)
                     /gene="abc-f"
                     /locus_tag="PLB33_01805"
     CDS             complement(27772..29424)
                     /gene="abc-f"
                     /locus_tag="PLB33_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HOG62286.1"
                     /translation="MLIIECSKIKKYYGDRLILDIDNLNIYSEDRIGVVGLNGAGKTT
                     LINILSQRIEPDKGWVKLRGKTEYFSQLEEPEVYEISSEMASKFGVETLWNENMSGGE
                     KTRFKLAKALENESILIFADEPTSNMDIEGIELMEKKLTEYKGALIIISHDRSFLDKL
                     CNKIIEIENGKIKTYEGNYSDYSRQRLHDKERAQFEYEEYIKEKERLGSVIADTKQKI
                     KSIKRPPKRMGNSEARLHKMGGQKAKANLERKVGNLEKRIEHLEVKEKPKKISKIKID
                     IMPSDYIHSKIIIEGKKVNKSFGNKIIFKDAEFCIYNGSKVALIGPNGCGKSTLVKMI
                     IDKDNSIRLSKGAKIGYFSQDMNILNNSLNIIDNVMESCNYSENFARLLLARLLFKGD
                     DIYKKIDVLSGGEQIKVSFAKILLQDINLLILDEPTNYLDINSLEVMEDTLKEYDRTL
                     LFVSHDRSLISKVADHIMTIENYKIKTFNGTFAEFKEIQSKKKLDKDVNMQIILLENR
                     LSDIVGRLSMQLKKEELEALDKEYYEVLEKLNKLKTLRTTPV"
     gene            complement(29722..30228)
                     /gene="lepB"
                     /locus_tag="PLB33_01810"
     CDS             complement(29722..30228)
                     /gene="lepB"
                     /locus_tag="PLB33_01810"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOG62287.1"
                     /translation="MKTFINEWVIPSIIAVVIVLFLNRFIFILVTVPTGSMETTIMPG
                     DRLYVNKLFDIKDAKRGDILVFQSDELDARLVKRLIGLPGETVEINATGEVFINGEKL
                     DEPYINNANGEERTFKIPEGTYFFLGDNRPVSVDARYWSNPFIPEDKIIGKVAFRFFP
                     FNRIGKVE"
     gene            30441..31247
                     /gene="zupT"
                     /locus_tag="PLB33_01815"
     CDS             30441..31247
                     /gene="zupT"
                     /locus_tag="PLB33_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOG62288.1"
                     /translation="MNNNILLAFGLTLIAGLSTGIGSAIAFFAKKTNTKFLAISLGFS
                     AGVMIYVSMIEIFAKAKDSLVAELGLKAGSWAAVASFFAGMLLIAIIDNVLPNEENPH
                     HLHNVEELNETKVHIEDNLMRTGIFTALAVGIHNFPEGLAAFISALQEPSIAIPITVA
                     IAIHNIPEGISISAPIYYATGDRKKAFKYSFLSGLAEPIGALIGYLILMPFLNDLVFG
                     IVFGVVAGIMVYISLDELLPSAQKYGDHHLSIGGLISGMAVMAVSLLLFI"
     gene            complement(31305..32471)
                     /locus_tag="PLB33_01820"
     CDS             complement(31305..32471)
                     /locus_tag="PLB33_01820"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOG62289.1"
                     /translation="MNNFTVPFSFTLPTKIVYGPGCISSLIDELHLNNGNRPIIVTDK
                     GVRNAGILDKITFLLERNNTDYIVYDGVEPNPKDVNVEEGARIARELGADSIIAIGGG
                     SPIDCAKSVGVLLAHKEDKIKKYDGKTAATKPLPLLITIPTTSGTGSELTFSSVITDT
                     LNNYKMTVKSQYTAAKVAICDPELTLSLPAHITASTGMDALTHAIEAYTATCAEPISD
                     AMALYATELIYNNLVTAFNDGVNLEARSAMLLGSMLAGIAFSHSDVASVHCIAESLGG
                     VYDLPHGVCNAVFLPHVMEYNMEYSVKRYARIAKAMGLSFENEREGAKKAVEAIKKLA
                     VDVKLPLFSSLKVDINDFEKIAEMSVKNISTESNPRPMTKEDYLTVIENAYKHK"
     gene            32642..33751
                     /locus_tag="PLB33_01825"
     CDS             32642..33751
                     /locus_tag="PLB33_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG62290.1"
                     /translation="MTQMIYTVQPGDTLYSIARLYGSTIQAIVDANNILNPNLIYPGS
                     VILIPVEEEYLETPPGSLIYTVQPGDSLYIISLLFKVSIQRILELNDIPDPSLIYPGM
                     KIILPPEALNPFQPIVPGIIRYTVLPGDTIYKIAARFGTTAQSIINANPGLDPRRLIP
                     GTVITITIPENAVAIYRGNPNRRMVALTFDATYGDNQTYELLEILRNNNIKATFFLSG
                     IWLINYPDLARAIAAEGHEIGNHSLTHPHMPLITMQEVTNQIVRTEALIRNITGQDPY
                     LFRPPYGEYTQAILNQLASLGYVTILWTIDSLDWQNPGAAAIVSRVVNNAEPGAIILL
                     HQSAPDTLEGLQSMITQLRQQGYDFGTVTQVISPL"
     gene            complement(33822..34604)
                     /gene="thiD"
                     /locus_tag="PLB33_01830"
     CDS             complement(33822..34604)
                     /gene="thiD"
                     /locus_tag="PLB33_01830"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOG62291.1"
                     /translation="MKKVLTIAGSDSSGGAGIQADLKTMTSHKIYGMSVITALTAQNT
                     TGVYGVFESSPEFVAQQMDAVFTDIYPDAIKIGMVSNKEIISVIADKLIEYKAGKIVL
                     DPVMVATSGGKLFKDDAVEMLINKLIPLATVITPNIQEAQVLSGIEIESKEDMQRAAV
                     KISKNYSGYILIKGGHLTLSSDDLLYSNGEFIWYEQPKINNLNTHGTGCTLSSAIACN
                     LAEGNDVKTSILNAKKYITGAIKSNLNIGKGRGPLNHCWNLG"
     gene            complement(34615..35253)
                     /gene="thiE"
                     /locus_tag="PLB33_01835"
     CDS             complement(34615..35253)
                     /gene="thiE"
                     /locus_tag="PLB33_01835"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOG62292.1"
                     /translation="MKLDSKSLLLYAITDRAWLKGKTVAEAVEEAILGGATCIQLREK
                     NLSDEEFLKTAMDVKAVTDKYNIPFIINDNVDIAVRVGADGAHIGQDDEEIKSAREKL
                     GADKIIGLSAATVEEAVQAEQSGADYIGVGAVFNTLTKLDANTVSFETLKEICNTVKI
                     PVVAIGGISKDNALQLAGTGIEGISVVSAIFAQNDIKTAASELLELAKKIIE"
     gene            complement(35237..36067)
                     /gene="thiM"
                     /locus_tag="PLB33_01840"
     CDS             complement(35237..36067)
                     /gene="thiM"
                     /locus_tag="PLB33_01840"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOG62293.1"
                     /translation="MFSEIIKNVKNNTPLVHNITNYVTVNDCANILLACGGSPIMADD
                     IEEVEEIVSISSSLVINIGTLNSRTIESMIKAGKKANEQNIPVILDPVGVGASRLRTD
                     TANKLLNEVKFAVVRGNISEIKALGNTNAKGAKGVDADLADSINEENLDEVVEYIKSL
                     SEKLSSVIAVTGAIDIVSDSKKTYIIRNGHPMMGKISGTGCMLSSIIGAYCGANKDNI
                     VDATAAAVCIIGLSGELAYEKILKNDEGTSMLRSHLIDFMSKIDDKILEEGAKFEIRQ
                     "
     regulatory      complement(36114..36213)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(36263..36904)
                     /locus_tag="PLB33_01845"
     CDS             complement(36263..36904)
                     /locus_tag="PLB33_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOG62294.1"
                     /translation="MKQFKYKIFDLDGTLLDSMDAWLNLGKDYLIKKGINPPDNLYEI
                     ISAMSMIETATYFQEAFKIDLSTDEIIKEVKKMIEDKYRYDFKLKPYVKEYLQKLENE
                     NSIMCIATATPLKLAEAALKRNGIIKYFSFIISCDEVGVGKNKPDIFYLAANKLNAKP
                     SEIAVYEDADFALITAKEAGFYTIGVYDDYFKNARKDIELISDIYIESFKELL"
     gene            complement(37013..37525)
                     /gene="thiW"
                     /locus_tag="PLB33_01850"
     CDS             complement(37013..37525)
                     /gene="thiW"
                     /locus_tag="PLB33_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HOG62295.1"
                     /translation="MIKNKEINLKKLVFASLLVCMGYVLNTFIWIPGMAPFQHFINVT
                     AAVFLGPAWAFLCALTIGLLRMIINGRTILALIGAIVGAFLSGILYKRFQKLYWAVIG
                     EVIGTGIISAILSYPFMRMFYGLPKGSIFMYIPLFVPSSIMGAMVAVLLIKALKKTNT
                     FDKIKAQLDK"
     gene            complement(37651..38832)
                     /gene="cytX"
                     /locus_tag="PLB33_01855"
     CDS             complement(37651..38832)
                     /gene="cytX"
                     /locus_tag="PLB33_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HOG62296.1"
                     /translation="MGSYKTSTLSNGLLWFGAAVSIAEIFTGTLFVPLGFSKGFIAIV
                     IGHIIGCILLYYAGIIGAKSGKSAMESVGLSFGNKGRNFFAVMNVLQLVGWTAVMIIQ
                     GARAMGVMLNTNLGMQSDLLWGIIIGAAILVWVVIGPKNLEKVNIFAVGSLFILTIFL
                     GATVFNRGSNVNISGEMGFGAAIELSAAMPISWLPLISDYTRDAKHPITATRVSVITY
                     FAGSVFMYTIGLGSAIFTGESDIAVIMYKAGFGILGVLIILFSTVTTTYLDVYSAGVS
                     LKTIHAGLPEKTMAIAVGLIGTAIAIFTPVEQYQNFLYLIGSVFAPMISILIADYFIL
                     KKDNIKKSVDVTNFIIWLIGFTIYRLLLNMNLAIGSTIPVMIITVIICVIADKIRKLK
                     V"
     regulatory      complement(38883..38984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT        12012 a         7644 c         5905 g        13586 t
ORIGIN      
        1 gttttttgga aggaagcgga tttgaagttg aaatgacaag atatgatgac agcggtctat
       61 acacagaact ttccggcaca atcagagata aaaaaaatga agatttaaaa aacagagttg
      121 aactgattaa taactcaaat gcagatttag taatatcaat acatttaaat tcctttccgc
      181 agaaacaata ctacggagct catgtttttt atcaaaaaag caatgaagca actacaaaat
      241 tggctgctga tataatacag gaaagcatga agaatattct tgataaaagc aataaccgtg
      301 tacctcaaat taaaaagggc ataaaaataa tggatgatgc taaggttccg gtgattttaa
      361 tcgagtgcgg atttttatca aacaacagcg aagaaagaaa actaatatca gaagattatc
      421 aagaaaaaac tgcttgggct atatatacag gtttacttaa atatttaaat gaattataat
      481 taatcaataa aattaaatac actttccgtt gcgggatgga atagtgtatt ttttttgtaa
      541 tattattgaa aaaaatgtga aaattttaga gattttctaa ataaatgttg taattctaat
      601 agcaatatat tataattaat taacaatact tggaggataa atatgagttt cgaagaaatg
      661 gagcattttt taaataatat taaatctgtt ctttcatcta aagttataat cgattcaaac
      721 aatgaaatcc gcgaaataca tgtactggct gacaatagca ggcattccaa gcaaattgca
      781 agagatatac gcactgcact gctttcgaag ttcaatgaag atgttgatta taaaataata
      841 agcgtagcac aaatagacaa aaatatgtcc ttcaactcgg attttagact tttatacgaa
      901 ggttatataa atgaaacaac tccggaaaaa attaaaataa aaactaaact cacctgggat
      961 ggcaaagaat ttataggaga agccgagggc tttaaatcag ataagcaatc cctgatagta
     1021 gctgcaagat caaccttgga tgcaatcaga caagctattg aatttgattg tttcatttta
     1081 gaagacattc aatataccaa aatagccgga gaaaaagctc tgattgttgc aattaatcag
     1141 attaataacg gcaaagaaaa catttcagtc ggtgcatcaa ttattgaaga taaccaaatt
     1201 gattctgcaa taaaagcaac attaaatgca attaacagaa aaatctgttt attgataaaa
     1261 gattgatagg gctgaccctt aacatcaaat aattatttaa agaattagcg tagcgaaacc
     1321 ttcctgttct actagcgaac tgagcagaga atatctgtgg aggtttcaga tgaaaaagtt
     1381 atttcctata ttagcaacaa tagtagcaat ggtattagct gccggcgctg gatttagctg
     1441 ggtttagtca gtaatttatc aggtcagccc ttcatataaa ggatgaaaaa tgattaataa
     1501 taagcaaaaa aaagaaaata acattaaact ttatacatat atattttttc ttgcgcttac
     1561 tgcaataaaa ctgatgcatt atttgtttaa caactataca ataagcgatt atgttttgct
     1621 tattgttttt tcaatattaa cagctatagc tgaaacattt ctgatactat tgccaaaaat
     1681 aggcggcgta tcggtcagtt ttgcactcac cttttctgca atattattga ccaacccatt
     1741 aacggtatca atcatttcag caataggaat gatattaaga tgtccttatg tagacggaaa
     1801 aggcagagta catatattaa atactcccat ttataaaaca atttttaatg taagtcagta
     1861 tattataagt tttggagtag caggttttgt atatgaagca atagataaat cctttaactt
     1921 catccttatt ttttttaatc ctgttgctgc tactgctaca ttagtagtat atatattatt
     1981 aaatacattc tttatgtcaa tgctaatgtc catattgtta aaagaaaaac tagtatacat
     2041 atggaaaaca aatttctttt ctttgttggt aaatgtaata ttagtcgggc tattaggtat
     2101 agtattggca tttgcatatt atagttatgg ctcaggcgga ctgctcttgt ttttcatacc
     2161 ccttctactc gcaagataca catttaagct ttatttggac atgcgtaaga attattttga
     2221 aacgttaaat gttcttgttc gtgccataga agcaagcgac ccatacacaa gcggacactc
     2281 catgcgtgta agtgcatatg cggaagcaat agcaagacag gtgggactgc ctcagagcaa
     2341 gattgattta ataaaaagcg cagccttgct tcatgatata ggtaaaatcg gtatagacaa
     2401 aaatattctg aataaaacgg gaaaattaaa aaaagaagag tttgaaaaga ttaaatctca
     2461 tccggaaatc ggagcaacaa taattgcaga tttaagctat ttatcaaata ttacagatat
     2521 aatacgacat caccatgaaa gaaatgacgg aaagggctac cctgacggat tatgccattc
     2581 caatatatca ttagaaacgt ccatactgac cattgccgat tcctttgatg ctatgacaac
     2641 agacagacct tacagaagtg ctttaagtct tgaatctgct cttcaagaaa tcaaaaataa
     2701 tgagggtaca caatttaacc ctgatattgt agatgatgcg ataatcgctt tgaagaaagc
     2761 attctttaaa atagctgaag agaaggttga acatgtattt tgaaattata tttttttcct
     2821 tactatcaat aataacagca attgtagttt tactgataaa aaaaagagat tttgattttg
     2881 acagatacaa tataaaaatc agaggctata aggtattaat attggcggca gtaatcaaaa
     2941 ttgctgctga atttttgttt aacaaataca caaacgtaac cttgcttaga attttatctc
     3001 ataactgggt catttatttt ggacttcttt atatttcatt actaaacatt aaaaggtctt
     3061 acatgatgtt gtttttttta ggtgccttat ttaattttgc ggcaattgca gcaaatgatt
     3121 ttaaaatgcc tgtttatgta tctgagctgc tttcagatgt ggaaacaaaa aaaatgtttc
     3181 ttctttccgg aaatgattta gtgcattcac tattgacgaa agaaacaaaa ttaaaatttc
     3241 tttgtgatat aattacatta tatcctccct atccttttcc aaagactata agtgtcggcg
     3301 atgtgttttt attatccggg atttttgcat tttggcaaga cttgttttat gagagctcaa
     3361 agattttaaa acattaattt acaaacaagc agggcagcaa agattgttgc tgcatgtgat
     3421 agaagccccg cctttattgt atatctgtaa tcggaaatac cgaccgagcc gaaatatact
     3481 gcggttatat agaatattgt ttccgatgag cccataatta ttgatgcgct tcttcctata
     3541 tatgaatcag ctccgtaatt atctagtatt gtttgaaata caccgaatga acctcctccg
     3601 gacaagggtt taataattga cagcgataat agttcagtcg gaaatcctat aaatgaagtt
     3661 ataggtgtaa gtttatctac aataaaagat tcagctcctg attgtataaa tagatttatt
     3721 gcaaatatga ttgctaatat ataaggaaat attttcagag ccgactttgc gccgtctatt
     3781 gctccctcaa caaatgcaga atatacatct acacccttta ttattccgta aaacaatatc
     3841 aaacctatca gtataggcaa aaataaatct gatatattca taattctttc ccttcataat
     3901 atttggcaaa tattattgca accatggttg aaaatgcagt tacaagcaaa gtaggcacca
     3961 taatttccga aggagccacg gagcctgaat cagctcttaa tttaataaca ttcaatggta
     4021 taagctgaat cgaagaaaca ttgattatca aaaacatgca catggccttt gtagctgttt
     4081 ttttatttaa attgctcttc tgcatttcct gcatcgcttt aataccaaaa gcggtggcag
     4141 agttccctat tccaaacaga ttggcagcca atgtcattaa aattgcattt acggcattag
     4201 cgtcattttg tacatctttg aacaaatatc tgaccaaggg ctttaaaaaa gatgataatt
     4261 tgtttaatat atttgttttt tccactatac gcattaaacc ggtccaaaat gccatgatgg
     4321 gtaataaatt taatattagt tcgactgact tttctatttc aaaaaagaga acatttgtaa
     4381 ctgtttccgg acttttatta ataaaagaaa ataaaagacc acaaaaaaca aatccaaacc
     4441 atagtttatt cataatatcc ttcctgcttt gtaattggta aaagatactg cgcaacaaaa
     4501 aaatcaaaca gacatattaa ttaatatgtc tgtctttatt attaattcat atttattttt
     4561 aatgtagaga ttcttcagtt ttaatccttt ctacctgaat ttcaagtacc ctttttgctt
     4621 ctgcctttgt aaccgtaata tctaaatctt tataaacaat cctcccgcct tcttccggta
     4681 taccctcaaa caacattgtt ataaaaccgt taaccgtagt aatatcaagc tcctcatcaa
     4741 tctcaacgcc aagcagctca aacatatcat caatatcagc attgcaggat actaagtatt
     4801 tgtcatctcc gattttcttg aaccattcaa ttacttcgtc atgttcatcc caaatttcac
     4861 caacaagctc ttctattata tcctccatgg taatcaatcc ctcagttccg ccgtattcat
     4921 cgattacaac agccatatgg actttagaaa gctgcagttc ctttaaaagc tctgatattt
     4981 tcttgtttgg tgtaataaaa atggttttag aaataagttt tttaatatct ttttctttcc
     5041 tgtttaaacc ctggtaaaaa tctttttcat gaagtacacc tataatattg tccatgtctc
     5101 ccgagaatac agggagcctg gaataaccgc tcaatataaa cttttctttt attgactcca
     5161 agctttcatt ttcttcaaca cctacaatat caattcttgg ggtataaata tcctctgcca
     5221 ccgtttcatt aaactcaatt gccgagcgaa ttaaatcact ttcattttta tttattactc
     5281 cttcattttc tacctcatcc agcatggttc gcaattcatc ttcataaaca gaaggttgaa
     5341 cttcagcttt aaaaccttta taaatcattt ccttacatat tgcaagaaga aaattgaccg
     5401 gagaacaaat ttgcaaaaaa atgctcattg taggagcaga aaacattgct gctttctcag
     5461 gagctatatt agccaagccc tttggaatta attcgcctaa aataattatg aaaaaaattg
     5521 caagtacggt tgaaattaaa atttcgttgt taataaacat ttttgaaaaa ataaagataa
     5581 ccaatgatgc agctgtaaca ttaacgatac ttttaaaaat tattatagtt gacataaggt
     5641 tgtcataatc atcatccagc ttcaaaacca atgctgcttt cttattatta gatgccaagt
     5701 ttttcattct cgatttgtta aatttttgaa aagcagtctc tgaagctgaa aaatatgcag
     5761 atatcaataa aacaattatt aatatcgcga cctgacttat accgtcatca tccatttttg
     5821 ttttacactc ccaatttatt tttattacct atagtataat tgattaatac gaaagtgtca
     5881 attcatttca accttctttg atatttgcaa gactaacatt aggaaaatta aggaagacga
     5941 taaaagcata aaaaagcata ttattgaaac aataaaacta ctttcacaaa tatataacaa
     6001 gaattattaa cattataaac caaataagtg cattttatca tgatattgca caattaggat
     6061 taaggtatat ttaaaacata tttaaggaat gcttaattga tttttataat ggaaatgttt
     6121 atagtcacgg tagaaaataa atggaggtat gaattgttat cgtttataag tattttcaat
     6181 ttaataatat ttattatatt tacagttttt tattcgtatc aaatatttta cgtatttgtg
     6241 gcattgttta ataaaagcaa aaacttcaat gcttcaataa atcataaata tgcggtggtc
     6301 atagctgcaa gaaacgaaag tgctgttatt gctcagttaa taaatagtat aaaaaagcaa
     6361 aactatcccg gtgaactgtt ggatatatac gttgtggcag acaattgcac ggacaatact
     6421 gcagcggtgg caaagaaagc aggtgcaata gtattcgaaa gattcaataa gcagcttgtg
     6481 gggaagggtt acgctcttga ttacgcattt aaaaatatat taaaaagcaa taaatgctat
     6541 gaaggatact ttgtattcga tgcggataat ttgttggatg aaaattatgt cagtgaaatg
     6601 aacaaagtat ttgacaaggg ttataaaata atcaccagct acagaaattc caaaaattat
     6661 gatacaaact ggctatcggc aggatattct ctttggtttt taagagaagc caaatatctg
     6721 aataattcaa ggatgatatt aaatacaggt tgtgcaatat caggcacggg ttttatggta
     6781 agcgatgaaa taatccgcaa aaacaacggg tggaagcatc acttgctcac agaggatatt
     6841 gaatttacaa tagacagtgc attgcaggga gaaaaaatcg gatattgcgg cagtgctgtt
     6901 ctttatgatg aacaacctta cctgtttaaa caatcatgga accaaagact aagatgggct
     6961 aaagggtttt atcaggtatt tgccaaatat ggcattgatt tgataaaagc aatttttaaa
     7021 aaaagaagct tttgctgtta tgacatgttt gttacgataa tgcctgcact attcctgaca
     7081 ttaataagcg tagctgtaaa tttgactctc tttttgatag gtttgattaa tatagaaaca
     7141 taccctgaac taatgaatga aacaataggt gcaatattgt tatcagtgtt aaattcctat
     7201 atagtactgt ttatattagg gcttatcaca accgttaccg aatggaaaaa tataaattca
     7261 tcccccctaa agaaaataaa atacatattt tcatttccga tatttatttt tacctatata
     7321 cctatttcaa tagttgcatt atttaaattt aagaaaattg aatggaaacc tataacccac
     7381 agcatcgtta aaacaatcga agaagttcga caataaaaga tgccctaacc ctattttttc
     7441 aagccaaagc gcagaaaaaa tagcggtagg tcattcgcaa cgagttccca aaatatgcga
     7501 ccggagggag ctgatatttt ggtgaacgag actgcgagaa ttctggagaa ttccttattt
     7561 aaatgtccaa aatttgctta ttaaataatt gcttatcatt accagtatat ttgtccacaa
     7621 ttttgccata tattcgctta cggaaaaaac ttctatcaaa ataatcatag ctcctatgtc
     7681 taaggcatat gtaaacaatc ttcccaatga aaattttata aattctttaa aatatgattt
     7741 gtcgcttaga aaaacatatt ttttatttat tataaatgca aagattactg aaattataaa
     7801 tgcagctgta ttagatattt tgtagtcgat accgataaaa agcattgcat aataaacaat
     7861 gaagtttacg attgtggtta gtattcccgc tatgaggtaa gcagccaatt cctttgaaat
     7921 cccattcttc attacaattt tccgccgtct tcaatttttt cgttgttgtg ctcatctaca
     7981 atataaaggg gtcgatactt tgtttcatta aaaattctgc ctacatattc gccgataatt
     8041 cccaaacaca ctaactgaac tcctcccaag aataaaattg taaccatcat tgaaggatat
     8101 ccttgtaccg gatctttcca aattaaactc ttaataagaa caataaacat gtaaacaatg
     8161 ctgaaaattg ataccagaat acctaagatg gtggatattc ttaaaggtga tgttgtaaat
     8221 gaagtaaccc cctccaaggc caaattaaaa agcttcagat aattccattt agtcttgcct
     8281 gctgctcttg gtttcctgtc aaataatatt tcttttttgt taaagcctat ccagctgaac
     8341 attcccttgg tatacctttg tttttcccga agtttcttta aagattcaat tgcccttctg
     8401 tccaaaagcc tgaaatcgcc tgtatcttct tgaataggta ttttggtcat cttccggagc
     8461 agtttataaa acgcggcaga agtaaatttt ttaagccacg tttccccttc cctgcttctt
     8521 ctttttgcat atacatcatc atatcctttt tcccaataaa aaatcatttc ctcaataagc
     8581 tcgggagggt cctgtaaatc tgcatccagt atcaccgttg cgtcacctgt tacataatca
     8641 aatcctgcag ccatagccgt ttctttgccg aaatttcttg aaaagctaat gtaatttacc
     8701 cttgaatctc gtttccgcaa atctctgaga atatttaaag tgttatcgct gctgccgtca
     8761 ttaacaaaaa gtatttcgaa ttcatagccg gaaattttat caatcacctt atttaattca
     8821 ttatataata agggtattgt ttcctcttca ttgtatgcag gtatcaataa gctgactttt
     8881 ttcatattac ctccttgatt tttctatggc aatagggctt gtataagttc cgtcaccctc
     8941 tatagcactg acattttcat ctatgtatac tgcgggaatc acgcctgctt taacatttgc
     9001 atccttgtgg taaacaaatc cgtccttatc gactattctc accatgcttt cactgctgta
     9061 atacggagtt ctaagccaat acctttcttg cttttttaaa cttatcttat tttcatatac
     9121 atatttttgt agctgatata catcaagtag aaaaacactt tctgcggaat aattgtaata
     9181 tgcatcagta ttataatttt gactgacata tgaagtaata gaattccagt aaaaaggttt
     9241 gttgcctcct atactttttt caatattgtt atagcttaat atatttttta gtttaacatc
     9301 ctttataaga agtttttctt catcgttaaa attactctta aattcaaaga gccatcttct
     9361 cacatctgat tcaatccagt tatttgagcc gtaaatatta tctgaatttg tttcatctga
     9421 ataatcaaat tccatatatt caaccgtatt atcacaccat aaaagatagc cgttttcatc
     9481 tttattcaaa actacccact ttagaggttc atttttatag ctgcctaaat aaatatattg
     9541 tcctattttt aatgtccctg tttcttttat ttcttcgtta gcagtctgaa taaatattgc
     9601 tatgcaaaac acaacaaaag ccgttagaac aaccgctgaa accgtctttg tgtagaaatt
     9661 gtttataagc caacatatgc ccactaaaat cattatatca aagcaaaggt tgaacaaaag
     9721 catatgcttt tgcaaatcag cttcaccatt tccaataata ggaagcacga attggctcat
     9781 ggttgtcaaa aacaatagta gtgctgcaaa agcaaatgca gtgttttcat aatcgtactg
     9841 ttttatatta ttgataattt cgtatatatt tattataaaa aataagactg aataagaaac
     9901 tattatataa aaggcatagc ccaatgcatt tttccgaata ttgctccaca aagaaaatct
     9961 ctcggtaaat gacagccgtt ccggttcatc ctcctttgaa cagtttccta aataagaagg
    10021 tcttattatt accgagctgt cagctgacag cttaagcttt tcaacggctc ttgaaggatt
    10081 taaaaagtaa aatttcagta tgtccaaaaa tgttgcatta tcatatactt ccttttgaaa
    10141 ttcatcgctg taaatatcaa actccccatg gtccaaaaat ccatgtgtgt ttgtaagagg
    10201 aaggtatttt aaaggaattg aaagcttttc caaatccttt tccggctcat ttgaatcttt
    10261 tgtaataccg aagaaaatac tttgataatt gttaacctga ctcatccatc gcggtgcatt
    10321 agtataataa aaaatcgaaa aacaaattat caaaatagag cctattaaaa ttaccgctct
    10381 gctaagccaa tctgctttta caataaacag ggcaagtgaa aatataccta tcagtatccc
    10441 tattggagtg tttgcaacct tagcaccggt tagtgctaag gcaaatatat aaaataaaat
    10501 tacataaaaa agacttttaa ttttactttc agtctttata attgcagtaa gcaagcccaa
    10561 tgttatcatt aaagaagcaa taatagctgc ttctccgaaa aaagaattaa aatatagcaa
    10621 atatcccata tcgcacaaaa ttacaagagc tattatcaaa aataaaaaat ttatgtattt
    10681 atatttaaaa tttatatttg taagaaataa acatagtccg taagaataaa ttgcagcata
    10741 aagaatacca agtactttta tattaaatat ttccggcgac tttcctaata caatttttaa
    10801 taaataattt ataatcattg atactttaat aaaaataaat tgggttgaaa aatactcatt
    10861 gtcattttca accttcggac tgataatatt tgacaaagag ccttttatat cttcacttga
    10921 catatcattg acagtataaa aattatatgc ataaaaatat ctgttgctta tttcgggctt
    10981 aaatcccaag ggctgaataa ctctgtagta atcgctgttg tcggcaattc ctatgaaagg
    11041 ctcttttaat aacaagacta aggagaaagc aaatattaat atgaaaatgc ccaatctttt
    11101 tatactttcc attttaactc caacctaacg atatagtaat attatggcat agataatttt
    11161 ttttgtaaat atatatcgac ataaaaaaag agccttattg gggtgccagg cagcaaactt
    11221 ggttgcctgg caccacgaaa atggcaccgc gaaaagaaat cgccctaaaa ttttctattt
    11281 atatactcta tctacatatc cgcttgttgt tgctcttaac aagcttgaat agctgagttt
    11341 aaagttaaca atatctccta ctttatattt catgtccgac tttgaaatat cgagtatcaa
    11401 gtggtcagag cttgctccca gaatatctat tttagagtct attgggtgca aggcatcagg
    11461 gtcaacatcc tgcctcccca ccgctataat tgctcttttg attatacctc tgtcttcata
    11521 tacaggttta ttgccaaaag catctacgcc ggtttcacct atgggaacag attgtttttc
    11581 ttttaattca actatttctg cttccaaagt aaatgcatcg tcataaaatc cttcaagctt
    11641 ctggctgtaa gcagtttcac cgcctaaaag gaaagcctct ccaactctta aattattaat
    11701 tccttcgggt agttctttcc ttcctatcaa atatatggag cttgaatttc ccccggatat
    11761 catttgtaat tttatatcga atttcttttc aatctttctt gcaatttcaa cgagtattga
    11821 aagattatca ctttttggaa ttactgctcc ataacaggtt aggtttacac ctaatccaaa
    11881 caactcaata ttttttagat ttaatatttc ttcaacagta tgatatattt cgtcttcgtt
    11941 tttaaagaat ataccttctc ttaagtctcc caggtcaatc atcagaagaa ttttatgaat
    12001 tttgttttgt cgttttgctg attcgttcaa gtttttgatt gtctttaatt ccgagtttag
    12061 agaaatatct gcatatttca ctacctcatc agtttcggaa ttcatgggca atcttaataa
    12121 aactctttcc ttcttagctc cttcgtattt tttgaggttt tggatgcgtg aatctgccaa
    12181 ataatctata tccgagccat ctaatatttt gaatatttcc atatcggcgg aataaccttt
    12241 tgttacaatc atcagcgagc tgcctgagcc tttcaccatt tcggttattc tatcaagatt
    12301 gttttttact ttttttaaat cgattactaa tcttggatac atattatcac ctacattctt
    12361 aaactatttt tcataaggta caaagcatca ctcaagtaac ttttcgacaa tactccaaga
    12421 gaagacaata tataatcttc atctattaaa actcttgcag cttccgtcgg atacaattca
    12481 tgctccttag ctaaagtttt tactgattcc agaatatctt tcttgtccgg taatcttgta
    12541 agcgagccgc cggttcctat gatgtattga attgcggtta aatcctttcc ttcagcaacg
    12601 gtttttttgc ctgatgcagt atagaaatat ctcaatttac ccgcatgcct ctccagagac
    12661 gtatgaactg cttcctttgt aagcctttca acaaattgtt tttccctgtc ggtttttgga
    12721 atcggcttat aattttccag gagttcttct gcatcgggaa attctttttt caaattatcc
    12781 atgcctattt tttcaacaat gtgagaaaca ttcacatata ctccaaggtc tccttcaacc
    12841 gttctttttg ccacaggctc cggactgatt aatattgaat ttatttcgct ggagccctcc
    12901 gttacggaat gaagatctgt cgttgctcct cccacatcaa atattatcaa gtctcctatt
    12961 tcttctctta atacttttgc agcttccatt actgctcccg gagtaggaat aataggtccc
    13021 ttaactaggt ctcttacctt ggccataccg ggcgcatgaa taatatgctc ttcaaaagca
    13081 tcctgaatta tttttcttgt aggctctaca accaatgtat caattttggg atatacattt
    13141 tctacaatat aaatctgatt gtccgcttct tcgcacatca ggcggacctc ttcctggttt
    13201 tcaatgtttc ccgcgtaaat tatcggtatt tttaaaccca ttgacaaaat catttccata
    13261 ttgtaaagag ctgtatctct ttctccgtaa tccacaccgc ctgcaactaa gattatgttg
    13321 ggttttatat cctgtatctt ttttaaatct gtttttctga gcctgcctga agtaatcatc
    13381 tttatgattc ctccggctcc taacgcagct tctttagctg ctcgaaccgt catatcatat
    13441 acaagcccgt gaacggtcat tttcaaaccg cctgcagcac tacttgtagc cagcatatca
    13501 tcccatgtca atgtatccgc ctgcaggttt ccctttaagt cttcaatagc gcccttaagc
    13561 ccgattgtta catcaccctc tgatacggtt gtaggtgcct gaccctgccc tacaaaggca
    13621 gggttaccgg tacctattcc attgaatgca ttaactactg tagtggtgct gccaatttca
    13681 gctaccaata catttacctt cattatgcct tcttttccct tcttttcttt actaagaaac
    13741 tggcaacgtc aataccgtgg gttccccttc caaaaccggc atcaagccct gcttccaaag
    13801 ctatttcatt tgtaacctgt gtgccgccgc agataatcat tactttatct ctgatacctt
    13861 tttctattgc taaatcatga acttttttca tatttttaat atgaacatcg tcatgagtaa
    13921 ttattgtgct tagaagaata gcatcagcat ttgtttctat ggctgcatca actaattttt
    13981 caacaggaca tgaagttccg agatattcat atttaatacc gaatccttca ataccgccgt
    14041 gttttatatc caatgtttct ttaagcccta ccgagtgttc atcctcgcct acagtagctg
    14101 caacaacaaa catcgggtta ttttgaatat cttctcttat ttctgcctct gacaataagt
    14161 caggtttttc agggataacc agctcgttta tatcaatatc aaagtttact ctgcctttta
    14221 cttcacaata agttccttcg gatggatgga tgcattgttt atgaacaacc gtcacgtctt
    14281 ccaagcccat tttttcaccg catttaatag cagcaaattc tgccgttctc tcatctaatg
    14341 gaaggaatat cgtcaacaat acgatgccgt ctgctctcca ttccacctca ggtttaacaa
    14401 ggtttgtatc atagtatttt tcaacttctt taagtcttac atttacatta tcatgctcat
    14461 ccaattcatc gatatactga attttggagc ggtcttcaaa tgtatctccg cctatggcat
    14521 cacttgcctt tttaaattca gcgggtatgt tgttgtaacc gtaatgtacg gatacgggag
    14581 caaaataatc ctcatctctt tcatatatga agccgtatcc gattccgcca tctatctgac
    14641 gtgcaatacc gtctccgttt ctctcgggat atttaccact gtcaatgaag aatcctcctt
    14701 ctacagcctt gaagtatcct ccgacttcaa gtatttcttc catgaacagt attgctcttt
    14761 ccttaagttc tcttacttct ttgcctaagg gtccttctct gtcaagctta accatttctc
    14821 taaagccgtc catgcctgta agagcttgct ttgcagtcat tgttcctgca atgttgttat
    14881 aatgccaagg cacgtttctt ccttcatcag gagtgatggt agattgaata tctgcacttg
    14941 taagccttga tataaccatg ttcaatgtat gagttaccgc tgcttcacga gtatccgatt
    15001 ccatatattt tgtattttgc tgagctctca ttttataatc tttgaacaaa tcacgaagag
    15061 ctactgcata tggtaagtct agtctcatac aaggtgcagg aggtgccgtt ggcggtacgg
    15121 tagacaatgc tatgttttct ggttttatgc ccactcttac tgaaaaggct gtatttatag
    15181 catgttgtac catcaattca ggcataactt tccaagcctt cattgctgta gcattggcat
    15241 tgtgagctcc gtctatttgc agcatatctg cccatgccat tatcttttta gcttcacaag
    15301 catcaacaaa gcttcttacc atgttaacat ttctgtacaa aacattgtat tgcgggtctt
    15361 gatgtgctcc gttaactcct tcttcggcaa acataactgc tatgtcggga cctgcaacac
    15421 ccgaaatata tgagtggaag tttatcggac gacctatttc atcttcaatt aaatcacaag
    15481 cttttcttga agctctcact tgctttcttg taaccataat accgccgata ccttggtttg
    15541 taccttcaag cagggagtca atatgtgact gaccggctgt ccttataacc ataagatggt
    15601 ctgcaccatg ccatgcagcc atacgcattc ttcttatatc atcttcaaat cttcctgaag
    15661 caatttcagt agtaattaca cagtcaggct gtgggtctat atatccaaat cctctgcttc
    15721 cgggaagggg aattgaattt tttaaacact ctgatgtttc atgatattca aagtcaccta
    15781 ctgttctttt ttcgtttctt ccttccctcc aatgccatcc gcggcgtctc ggttcatatt
    15841 tatctaaatc ttttaataat tcttttatat ctaatttttt gttaggttca agtttcatcc
    15901 tcatccctcc taaaatagtg tatcaacatc atcccagtgt ttgccttctg caagcaataa
    15961 tcctgcttca cgcactgagc agcctaattt cttcgataat ctccaaacaa cattgcctgc
    16021 accttttccc attaaaccct tattcattac accttcaact atgggtttaa cttcaagact
    16081 tgaaaatccc attcgaagca atatacttct ttcaacggat ggtgttgtat ttttctttcc
    16141 taattccaag agagggtcta caatttcatt tgctaatttc caaaatcttt catataattc
    16201 ttcatctgta agattcgcta agtgagctct tctttgctgg taatcatcag ctcttttcat
    16261 aaaatcctcc tgtaattttt aaatttcaaa taggcgtaat atttcatacg cctatttgtt
    16321 taatattcta caaagatgca attgtttctt ttacgaattc cacgctggaa tttgtttctg
    16381 ccgcaaggaa ttctatgtca gcttccgtag gtgttacttt gtaattattc agtgcattcc
    16441 ttattaatga ttttctcata gcgatcacat ctgcgtcatt tgctttaatc aatgaaggat
    16501 gagaaggtaa aattatattc tttcccggaa tttcatcctt tgggtctcca aatttaactt
    16561 ctattccatt ggctcttgca aatgacaact gaggctgaat atgcttgcct gcgcctgtat
    16621 attctgtttc ctgaacaact atcgtttgat cttggtctaa ttcctgtgca agtgcaaatg
    16681 cagctgtaag tgaagtattt ccggcaggac ctttttccaa tccttcaaga gatgctaatg
    16741 cttcagtcat atagaaaact tccccttgat gaactattaa atatctgtca atatatctta
    16801 atggtcttgc tgccgagcga ggaacatccg agcggtccgg ccatgttgaa aaaggcattc
    16861 cgaatcctgt atgtcctgtc gtaaatgatt ttctgttaaa ctgtgtgtct gatgccatat
    16921 gaagtcccga caaatctacg ctggcagcca ctatttgaga ctgagctcct gcctttttaa
    16981 gtcctcttgc agttcccgta agatttccgc ctcctgcatt tgttgccatt acaacatccg
    17041 ggtccttgcc aaacttttct ctgaactgca ttgcaatttc ataacccaat gtttctacac
    17101 cggctactgc aaacggagta tacaaggatg cattaaagta tccggtctct tccaatattg
    17161 tcaggaatgt gtagaataac tcggggccta ccgacagttg aacaacttca gcccccaaag
    17221 cctcacactt ccttgctttt tcgattattt caggctgacc tacacccctt gaatcatagc
    17281 attcctgaac tacaatgcat ttaagcccat acattgctgc ctgtgatgca actgcagcgc
    17341 cgtaattgcc gcttgtagcc gtaacaacac ctttgtatcc aagcttttta gcttgatata
    17401 ctgcggttga tgctcttctt gctttaaagc ttcccgatgc atttgctgct tcatctttta
    17461 tgaatatcct tgctcctttt ccttccggag ctatttttct tgctaatgct gttaaatttc
    17521 taagctctat aattggagta ttgccaactc cgtgttcaga ctgaattttt tggatttctt
    17581 caatagtaaa tccggcttct ttcatcattc tttcataatc aaaagctatt gagccgcttt
    17641 caaatttaga aaaatctaaa ccggccgaat ttttcattat ttcaggtctt cttgccataa
    17701 cggcagcata actcaaatct ttactcatta tcagcacctc caaataaaat ttcttttact
    17761 tgttcgctga ttttcaataa ttcaggaaca aagttgccga aatcatgctt gtaacatgga
    17821 tttacttcca gcaaagtgcc tttttccaac ctttttgttc ttgttaatac tgttacttca
    17881 tctccgatat ttgcatcttc ctgtaagtat ccttttgtcc ataactccaa aggaaccttt
    17941 ttagtatcat caggaacatg tggaactctt tcagccggct ctaaaacatt tctgtgtatt
    18001 aaaacccatt cacctttttt aaccattatt acgctcctta cctttatttt attaatttca
    18061 ctattgtttt ttcttcgcct taagctttaa aaataggatt aagtcatcat tattttacta
    18121 ttttgcattc cgggtcgatt aaatctctca tatctcccat gattgctctt ggtgccggga
    18181 tagtaagcat tgtatgtaat cccggtttag catttataat catcggaata gtatttacgc
    18241 acatagcaat agtaccgatt cctcccggaa cttcaggaga gttaaccaaa ttaatatttg
    18301 gagttccttt gattattacg tagtcacctg tttgaacacc aacctgttca ggctctattt
    18361 gctgtggatg gtccatttca attttcaatt cgccgtcaac atatccataa cccttcattg
    18421 cgcagcctgc aacatcccct gctttagcaa atccatgagg agatttccta tctacgtctg
    18481 taacaatggg ctccattgag gtagttactt tatcaacttt ccagcctata gcttctgaaa
    18541 tcataccgat tgattcatgg aatccgacgt gacctgatag tgttcctgtt tcaacacctt
    18601 ttttaaattc ttcaacagtg atgcctatac cttgctcatg cataactgca ggtccgaagg
    18661 gcgataagct gttaactctt cttgctgtaa tgtggtcaat tgtttggcag catcctgtca
    18721 taacaacaac caagtagtcc ataattaatc cggggttaat accggttcca attattgaaa
    18781 caccgttagc tttagctatt tcatccaatt gttttgcaag ttcaggattc tgagcctgcg
    18841 ggaatgccat ttcttccgca cttgatacta cgtttatttt tctttccaag cagaatttta
    18901 gtctttcaaa tgctacttta gtaaatgaat ccgtacagca tagaactaca tctgcagctt
    18961 tttctttaat aacatcttca ggagttccaa cgagtacttc aggtctgtct cctctttcta
    19021 ctcctataat ttcgtacatg ctttttccta atttggcacc tctgccgact gcaccaacta
    19081 tgtcaacacc tttcttttcc agaagcattc ttgccatgcc gcctcccatg gctccgagtc
    19141 cccaaataat tacttttaca ttttgcattt gttaatcctc ctaatatttt tctactctat
    19201 ttattattta ataagcaatt attatgccaa aaacattaaa ataaataaat ttattagtta
    19261 tttgatattt aactttaata tttcaaactc caagttattt tatctcaaaa taagcaactt
    19321 attccagtac attccctctt tatataacaa acaattcaga aataatataa aaaaagcaag
    19381 tatttttgca gctaggtgca aaaatatttg cttttaaaca ctaattttat atttctttat
    19441 tttatgctgt aaggtttgac gctttatttt taatgcattt gatgctctgg taatgttatt
    19501 gttattctct aaaagtgctt tctcaattat tcttttttct aaatttccta aatattcatc
    19561 aatgccggaa tcttctactt tttcataatc gtcaattcga ggctcaattg aacgatgcac
    19621 ggaaaaatga ttaacagtca aaatatattc atcatcttca cacatgctga cagctgacat
    19681 aatcatattt tcaagttctc ttgcattgcc cggataatca tatgataaga gatattcata
    19741 cacttcatcc gagaaacctg taacgtgttt gttaaacctc tcattgtgtt tatcaacaaa
    19801 cattttagca agcaaaataa tatcatcaac tctttctctt aatggaggta catttattgg
    19861 gattacatta agcctataaa ataaatcctt tcttagcttt ccttctttta atattttttc
    19921 gggaggctca ttacatgtag taataatcct tacatcaata ggaatatcag ttgaaccccc
    19981 aacccgtctt atatagtttt cttgaagtac tcttaacagc ttaggctgca attcatatgg
    20041 cattgaattt atttcatcta atagaagcgt tcctccattt gcctgttcaa ataatccggg
    20101 acggtcaacc gcacctgtga accccccctt tgttgtgcca aataataatc cttcaagtaa
    20161 agattccgga agagcagcac aattttgagc tttaaaaggc ttatcctttc tagcgctggc
    20221 ataatgaatg ctttgagcaa ttaactcctt acctgttcct gtttcaccgt aaataaatac
    20281 tgatgcagaa ttttttgaag attttttcgc tctttcaata acatccaaga aattttcact
    20341 gtgtccgata atattgtcaa aattatattt ctttactttc ttatgtttaa ccatttccgg
    20401 tttttctatt tcatcatgga gttttaatat tgtattggac atctcttcaa ttttagttat
    20461 attttttgct acttcaacag cagccacaac ctcatcatca attatcacgg gtattgtggt
    20521 attaattgta gaaatctctt ttccgtcctt gttcaaatat gtttgcttta aatttttcgt
    20581 tacggtttta gaatgcaagg cttttagaag agtgctgttt tcggtgttaa atcccttgaa
    20641 aacctctgta aaaggtttct tgattacatc agctgagtcc atcttttcca aagcagacat
    20701 ggcttcatta tatatgatgg tattgccatg cttgtcaagt acatgaacac cttcatctac
    20761 taactgcaaa attttttgca gcaacagtaa acattccttt gctttcatag acccctccaa
    20821 ttttaccggg gaccttcctc tattcttatc tatatagact cgcctataga cctgtcccca
    20881 ttctaatgat taattgtatc ataagaattc aatttttaca atgatgatag tttacttttg
    20941 tataattacg gtataaaatg aaattaatat ttgatgtaag gcatatgtat ttgtaatcct
    21001 aagcgtaatc gaggattata tgagataaac attaaaggag ttcaaatgaa aaaaatagag
    21061 agttttcaag taaatcatct aaaattaaaa cgaggaattt atgtatcgag aaaagataaa
    21121 gttggagaaa atattgttac tacttttgat ataagaatga aggagcctaa cagggagcct
    21181 gtaataaata ctgccgagct tcataccata gagcatttag gtgcaacatt tttaagaaat
    21241 catccggtat acgacacgcg cacaatatat tttggtccta tgggatgcag gacaggattt
    21301 tatgttattt ttagcggaaa tttgacatca ttggatgtat atgaaattat taaggaaatg
    21361 ttcttattca taatgaatta tgaaggagat attccgggag ccgatgagat atcgtgcggg
    21421 aattatcagg atatgaactt aaatatggct aaatatgaag ctgctaaatt ttataacgaa
    21481 gttcttttaa atataaaaga tgaaaatttg aattatccgg agtaaagatg ccctaacccc
    21541 atttctcaga gtcctttttc tgagaaatgg atggtaggtc attcgcaacg acttcccaaa
    21601 atatgcgacc atagggagca aatattttgg tgaaggagac tgcgctatgt cacaatctgc
    21661 tatatctgtt ttagattagt ttgatgtctt tattttattt gcatattttc ttctttctat
    21721 ttcggttaaa tattttttcc taagtcttat ggcatccggt gttatttcca ccaactcatc
    21781 atcattgata aattccaatg cagcttccaa agtcattatt tttggaggtg ataattttat
    21841 tgcatcatca gctgccgatg aacgaatatt agattgtttt tttgttttgc agggatttac
    21901 agtaatatct tctccccttg aattcatgcc tataatcatt ccttcataaa ctttgtcgcc
    21961 cggatttata aataaaattc ccctgctttc caagttgctc aatgaataag caactgcttc
    22021 gccctggtcg ttggatatca atactccgtt aattctttga ggtatttcac ctttaaattt
    22081 ttcatagtgg tcaaatactc tcaccatggt accttctcct cttgaatcat ttatcaattc
    22141 cgtgcgataa cccaaaagtc ctctggtagg aaccaaatat tcaattttta cgtaattccc
    22201 gtcaggcacc attgaaacca taatgccctt tcttaaatta agctttgata ttacagagcc
    22261 ggaatatatt tcaggcacat ttacaatcac tctttcaatc ggctccataa cgataccgtc
    22321 tattttttta agaataactt caggtcttga tactgcaact tcataacctt cccttctcat
    22381 attttcaaga agaattgaca aatgaagttc tcctctcccc gacactttgt aaccgtctaa
    22441 ggctccttcc aaagcttcaa ccctaagccc cacatttact tctaactctt tttcaagcct
    22501 tgcttttaaa tgtctggttg taacatattt gcctgataaa cctgcaaaag gagacgtatt
    22561 gattaaaaaa ttcattgaca tcgtgggctc ttcaattttt atggtttcca tcggtataat
    22621 tttgttttca ttgcaaatgg tgtctccgat agatatattg gcaattcccg aaacaattac
    22681 tatatcgccg ctgaacgcct ctttgacctg agtacgtttt aaaccttcat atatataaat
    22741 attagatatt ttttgctttt caacagtacc gtcatgcttt gcaaccgaaa caatctggcc
    22801 ttcttttatt gttccggcgg taacccttcc tatgccgagt ctgcctatat aatcatcata
    22861 ggcaagagtt gatacctgca gctgcaaatc ttcgtcatcc ctgtcgggat aaggctgaac
    22921 gtgatttata atagtgtcaa acaagggatt aatattgtgg ctttcctcat ctaattcata
    22981 ctgaacaatt ccttgttttg caataccgta cagtatcggg aagtcaagct gttcatcatt
    23041 tgcattcaat tctacgaata aatcaaatac catatcaacg acttcttctg ccctgtgatt
    23101 ttttttgtcg attttattta ttaacaaaat aggctttatt cctatttcga gggatttctt
    23161 aagcacaaat cgcgtttgag gcattggtcc ttcgatggaa tccaccagca aaattaccgt
    23221 atccacagtc ttaataattc tttcgacttc tgatgaaaag tcagcatgcc ccggtgtatc
    23281 aactatatta atttttatat cattatgcat taccgaacaa ttttttgaat aaatggttat
    23341 tcctctttct ctctccaaat catttgaatc cattatttgc tcggaaactt cctgattttc
    23401 tctaaaaacg ccgctttgct gcaaaaaagc atccaccaga gtagattttc ccgcatcaac
    23461 atgggcgatt accgctatat taataatttt attcttcata attacctcta cggggacaat
    23521 cctcccctct tctgactaag cagggcttgc tctgcattag agttatgtcg cccctctatt
    23581 aaatttttac agtttagttt aatcttttta ataaaatttt gcaagttttt tttgtcgtca
    23641 ccctgactca attaaaaaaa gggaacgtcc tttaatccct caaatatgaa ggtaaaggtg
    23701 agtccccgtc attagtccta tcgatacccc tgtctatctt ggtatgaatc cgactttctt
    23761 ttgagctttt tttaaggttt tatttgctac aacctctgct ttttgcgcac cggcattata
    23821 tatcttttca agataatctt tgtttttcaa caaataatct attctctcct gtataggctt
    23881 cagttcttca ataacaactt ctgcaacatc ttcttttaat ttagcatatc ccttgctttc
    23941 ataattggat acaatttctt caggctttat atttgaaatt ttcgagtaaa tatttattaa
    24001 attttttact cccggctgct catccgtata atttacaacg cctattgaat ccgtaaccga
    24061 acgcttaaac ttccttctta taacatcagg actatcggta atgagcacat atgaattttc
    24121 atttgaatct gatttagaca ttttgctctc agggtcctgc aagctcatta tttttgctac
    24181 atcggtcgaa atataaggct caggaactac aaatgtttca ccgaatctgt tattaaatct
    24241 ttgtgcaata tcccttgtta tttctaagtg ctgtctttgg tcttctccca caggaaccaa
    24301 atcagcttga tataaaagta tgtcagcagc cattaaaaca gggtaggtaa aaagtcctgc
    24361 cgttatggca ttggaatcct gctttttgga tttgtcttta aattgtgtca tcctgccaag
    24421 ctccccatag taagaaaaac aattcaatat ccatgtaagt tccgcatgtg ccgaaacgtg
    24481 tgattgaata aataatgtta cctgttccgt atttaacccg gcagctatat aattggctaa
    24541 tacttcatat gtattttttc ttaaatcctt aggctcctta ggtactgtta tcgcatgcat
    24601 atcaactaca caataataac agtcatattc attttgcaga gccacccaat ttttcaaagc
    24661 tcccagataa tttcccaaag tcaatgagcc tgtgggctgc atgccgctga atattctgtt
    24721 tttattttcc attttactcc tccgagaata tataagtaaa aacgagttcc tatttttgaa
    24781 actcgtaaag ctcaattttt tttgattata ctactataaa aataatttgt caacaattgg
    24841 tcaaagaggt gtgtccccca actaagaacg gggacattcc tcgctcctgc agggactatc
    24901 ccttcactag agaggtgtgt ccccaaacct tattacataa gtaataatca aaaacattat
    24961 ttgtgacact tctattataa acccttcaac tgatgacggc acatttttta tccttttatc
    25021 caatatgctt acaattaatc cggagagcat aaacataaga gatagcgaaa ttataagctt
    25081 atagtttagt attgccgcag atagaaaaga tatgccaaag gcagctatta tatggtaatt
    25141 gccgcagtat ttgagtatcg aagtattttc taaatctctt tttataacct tgctaagtat
    25201 catcaaattt gaaaatcctg cagcaggcat aataatcaaa gaagttacgg gtacaactcg
    25261 aaatagagaa aggtacatga aacagattaa aaccgttcca ataattcctg atatattatc
    25321 ggtttgattt gcaggcttta tataataatt taaagttctg taaacatcct ttatattggt
    25381 tgtttttgtt acaacacagt aatataaaag aactgcagaa ctgataaaaa aaccgtcata
    25441 tatatatcta aaggaaacaa taataaatgc tactaatcca atggcaagtc ctattatggg
    25501 aagtgcaatt ataccgtttt tataatcctc ctcatttgct tcttcataaa tggtaagact
    25561 taaacaggaa tattttttta tcattaatac aaggcctata acaatacttt ttaagtcaaa
    25621 cataatacta cctcaatttt atattattga atataatcgt ataggctgtt ataaaatcta
    25681 tttaggatta aatacgcttc ctgatatgta caataattaa acggtttgaa gtttaatccg
    25741 tctcctttta taaacccttt atcagctgca atatatatat agggtttttc attttctgca
    25801 acctgctcta tatcattaaa agatacggtc tgaaattctg aaatattcat acccaattca
    25861 ttggctatat atgctaatag ttttgctatt tcaagtcttg tcatatattc gtccggctca
    25921 taaaaggaat ctgtgtttaa aattcccatc tgatatgctt cataaaatgc attgcttgag
    25981 tttgccaaat aattataata agaagtataa ttcaacccat cggttacata aacttctccc
    26041 ctaagagggt ctaatgccac atgctcttca taatccgtta aattaaagta tttgtttaat
    26101 tcattttctc ctaatttaat gtgaagcatg tttaaaacca gtttatagaa atctcctctt
    26161 ttaatataag cttcatacag cccgttgaat tcatccggaa taagagactg atttttcatt
    26221 tccgtaattg tgtccttagc ccaatcctga ggagtttgag gtattgcatc aagccagagt
    26281 tttgtatttt ctgtaattga attaaagatt ttactgaatt cttcggcaat caatctgagt
    26341 ttttcatgta ttattgcata atccccgccg tttatattac gaagtatgaa cggatttgct
    26401 tccgcatacc atattaagtg tgcaatatca ttctcaaaac tgattgcgga atgccggttg
    26461 acgaaaactt ttgaatactc ctcgctccag gtaccgtatt caaatccctc atttaacttg
    26521 tcaaattcat cttttaaatt atcaagatca tatgtttcaa gcatataatc acttatgaaa
    26581 tgactaaaaa catgtgttat aacttgttca gatgcataat gagattcaga atacagactg
    26641 ccttccaagg tctttatgta aatattgact gagctttcat ctattattga taatgtaaac
    26701 aattctttta ttatttctgt tttatctaat attacattcg tagcaattcc cttgcttaaa
    26761 taatagtcgg taatttcttt aataatattg tcgggaaatt tcttaatact cctttccaat
    26821 attgacatgc aatcaataaa ttcgctgtaa tcaacacctt ccggtattat aatatttatt
    26881 ccgtaagtat tttcaatact ttttttaata tcaaattcat tataagcttg gctgaaacca
    26941 aaagcttgag ttcctaacat actaatacat attaccaaac atattatttt ttttatttta
    27001 atcatcttat ttacctcgcc gataaattat ttgattggat gaaatattgt taaaagtata
    27061 aaaattatag catagcttaa aacaaaatgc aatcaaaatt tgcaaacgtt tacgctaaga
    27121 gtgccatccg gagttttgct atgaataagc gttaattttc attctgtagg atttaactta
    27181 ctcttagtct ggtaaattca ataatataat ggtataataa tatattgtaa aaaatactat
    27241 atgattaata ttaacagagg attgaaatga aaataacaat aatatccgta ggcaaaataa
    27301 aagaaaagtt ttttactgaa gcaattaagg aatatattaa acgtctttca aaatattgca
    27361 agtttattga agagattgtg cccgatgaaa gggcggatga aagtttttca cctgcagaaa
    27421 tagagcaggt taagataaaa gaagggcaaa aaatacttaa taaaattccc aaaaattctt
    27481 atgtaattgt tttggatata aacggtgctc agcttagctc cgaagccttt gccgaaaaat
    27541 taaatacctt cggaatcgaa ggtatttcgg atataacttt tattataggc gggagcaacg
    27601 gactttggaa agaagtaatc aagagtgccg actttaatct ttcattttcc aaaatgacct
    27661 tcccccacca gctgtttaaa gttattttat tagagcaaat ttacagagct ttcaaaataa
    27721 attcaggcga aacctaccac aagtaataat tccgacaact ccgggattat cctatacggg
    27781 agttgttctt aacgtcttta gtttatttaa cttttctaag acctcataat attctttatc
    27841 caaagcttct agctcttcct tttttaattg catagacagt cttcctacga tatcggaaag
    27901 cctgttttct aacaaaatta tctgcatatt aacatctttg tctagttttt tcttactttg
    27961 tatttctttg aattctgcaa aagtaccgtt aaatgttttt attttataat tttcaatagt
    28021 cataatatgg tctgccactt tactgattaa gctgcggtca tgagaaacaa acaagagcgt
    28081 tctgtcatat tccttaagag tatcctccat aacttctaat gaattaatat ctaaataatt
    28141 tgtcggctca tccaatatta ataaattaat atcctgcaat aatatttttg caaatgaaac
    28201 ttttatttgc tctcccccgc ttaatacatc aattttttta taaatatcat cacctttaaa
    28261 taaaagtctt gcaagtagaa gccttgcaaa attttcggaa taattacagc tttccatcac
    28321 attgtcaata atatttaaac tgttattcaa tatgttcata tcttggctaa aatagcctat
    28381 ctttgcacct ttggacagcc tgattgaatt atccttatct attatcatct ttaccaaggt
    28441 actttttccg cagccgttag gacctatcaa tgctaccttt gaaccattat aaatacaaaa
    28501 ctcggcatcc ttgaatataa ttttattccc gaaggatttg tttactttct tcccttcaat
    28561 tattatttta ctgtgaatat aatcggaagg cataatatct attttaattt tagatatttt
    28621 tttaggtttt tctttcacct ctaaatgttc aattcttttt tccaaattgc ctactttgcg
    28681 ctctaaattt gccttggctt tctgaccgcc cattttatga agcctcgctt ctgaatttcc
    28741 cattctctta ggtggtcttt ttatactttt tatcttttgt ttagtatcgg ctattacact
    28801 tcctaatctt tccttctctt ttatatattc ttcatactca aattgtgctc tttctttatc
    28861 atgaagtctt tgtctgctgt aatcactgta attgccttca taagttttaa tctttccgtt
    28921 ttcaatttca ataattttat tacatagttt atctaaaaag cttctatcgt gagaaattat
    28981 tatcaacgca cccttgtatt ccgttagctt cttttccata agctcaatac cttcaatatc
    29041 catattgctg gtcggctcat ctgcaaaaat taatatactt tcattttcta aggctttagc
    29101 taatttgaat cttgtttttt ctccaccgct catattttca ttccataatg tttccacacc
    29161 aaattttgaa gccatttcac tgctgatttc ataaacttcc ggctcctcta actgtgaaaa
    29221 gtattctgtt ttacccctaa gcttaaccca tcctttgtca ggctctatcc tttggcttaa
    29281 aatatttatt aatgtagttt ttcctgctcc gtttaaacct accacaccta tcctgtcttc
    29341 agagtatatg tttaaattat ctatatctaa tattaatctg tctccataat atttttttat
    29401 tttactgcac tcaataataa gcataaaaaa tcctcccttt ctttctttgg aggatagcat
    29461 ttttacttta cacaatatat ctaaggatat gcataataaa tcttgcatag taataataaa
    29521 gtaacagcag ctattaaaat actatccacc tgtaatacaa taaaatgcac aataaaaaat
    29581 ctttaattta attgtcatta taattgtttt tcaagcggat tatttcattc tctatacctt
    29641 acctttctta atttctttac attacaaaaa attatatata ctgtcaagat tcttttattc
    29701 tgcatgttgt aaatagttct attattcgac cttacctatc cggttaaagg ggaaaaatct
    29761 gaaagcgacc ttcccgataa ttttatcctc cggtataaaa ggattgctcc agtaccttgc
    29821 atctacagat actggtctgt tatcgcccaa aaaaaagtat gtgccttcag ggattttgaa
    29881 tgttctttct tctccgtttg cattattgat atacggctca tcgagctttt cgccatttat
    29941 gaacacttca cctgttgcat ttatttcaac agtttctccc ggcaatccaa taagtctttt
    30001 tacaagcctt gcatccagct catctgattg gaatacaaga atatctcccc tttttgcatc
    30061 ctttatgtca aaaagtttgt taacgtaaag tctgtcaccg ggcataattg ttgtttccat
    30121 ggagccggtt ggaaccgtca ccagaataaa tataaatctg tttaaaaaaa gtacaattac
    30181 aacagcaata attgaaggaa taacccattc atttataaat gtcttcattt tcacgtcctt
    30241 aatcttattt atactgaact tattatatta cctgataacc attataacaa tttatttctt
    30301 atcaagcaat aaaaattcat aacttaaaaa gattgtaaga aattaggatt atagattgat
    30361 tatgctttta attatcttaa ttgctataat tagtttacat ttttatttaa ttgggtatat
    30421 aatagtttta ggaggtgcaa atgaacaata atattttatt agcatttgga ttaacgctga
    30481 tagcaggact tagcacggga attggcagtg ctatcgcatt ttttgcaaaa aaaacaaaca
    30541 ccaaattttt agcaatcagc ctgggctttt cggcaggggt aatgatttat gtttccatga
    30601 ttgaaatatt cgctaaagca aaggatagcc tggtagcaga gcttggctta aaggcaggtt
    30661 catgggcagc agttgcatca ttttttgccg gcatgttact tatagcaatt atcgacaatg
    30721 ttctgcccaa tgaagaaaac ccgcaccacc tgcacaatgt tgaagaatta aatgaaacaa
    30781 aagttcatat agaagataac cttatgagaa ctggtatatt tacggcactg gcagttggaa
    30841 tacataactt tcccgaaggt cttgcagcat ttatttctgc acttcaagag ccttcgatag
    30901 ctatacccat aaccgtagca atagctattc ataatatccc tgaaggaata tcaatatcgg
    30961 ctcccattta ttatgctaca ggggacagaa aaaaagcttt caaatattct tttctttcag
    31021 gacttgctga gcccatcggt gcacttatag gttatttaat attaatgcct ttcctaaatg
    31081 atttggtgtt tggcatagtc ttcggtgtag ttgccggaat aatggtatat atatcattgg
    31141 atgaattgct tccttcggca cagaaatacg gagaccatca cctttcaata ggcgggctga
    31201 tatccggcat ggctgtaatg gcagtaagcc tcttattgtt catataacct aaacccttcg
    31261 cttcgcgaag ggtttttcat gacaagggga cgggggtagt gtcatcattt atgtttgtag
    31321 gcgttttcaa tgacagtcaa atagtcttct tttgtcatgg gtctcgggtt gctttcggtt
    31381 gaaatatttt taactgacat ttctgcaatt ttctcaaaat catttatgtc tacctttaag
    31441 cttgaaaaca agggcagttt aacatcaact gctaattttt ttatcgcttc aacagccttt
    31501 tttgctccct ctctttcatt ttcaaagctt agacccatag ctttggctat tcgtgcatat
    31561 ctttttacgg aatattccat gttatactcc ataacatgag gcagaaatac agcattgcag
    31621 actccgtgag gaaggtcata tactcctccc aaagactcag ctatgcaatg gacagaagcc
    31681 acatcggaat ggctgaatgc tatgcctgca agcatgctgc caagcagcat tgcgcttctt
    31741 gcttcaagat tgactccgtc attaaaagca gtaacaagat tattgtaaat taattctgtt
    31801 gcatacaaag ccatggcgtc acttatgggc tccgcacaag ttgcagtata agcttcaatt
    31861 gcatgggtta gagcatccat gccagtggaa gcggtaatat gtgcagggag tgataaggta
    31921 agctccgggt cgcaaattgc aaccttcgct gcagtatatt gacttttaac agtcatttta
    31981 taattattca gtgtatccgt aataacggaa gaaaatgtca gctcgctgcc ggtgcctgaa
    32041 gttgtcggga tggttattaa cagaggcaag ggctttgtag cagctgtttt gccatcgtat
    32101 ttcttgattt tatcttcctt gtgagctaag agcactccaa cagatttagc gcaatctatc
    32161 gggcttcctc ctccaatggc aataattgaa tctgcaccta attctcttgc tattcttgct
    32221 ccttcttcta cattcacatc ttttggattt ggctcaacac catcataaac aatgtaatct
    32281 gtattgtttc gctccagcaa aaaagttatc ttatccagta tgcctgcatt tctaacacct
    32341 ttatcggtaa caataatcgg tctatttccg ttgtttaagt gcaattcgtc gattaaagaa
    32401 gagatgcatc ccggtccata cacaatcttt gtcggcaaag taaaactaaa tggcacagta
    32461 aaattattca ttcaaatcct ccttggtaat taacgatatc cgtagttaca tatctttatt
    32521 atattacatg tccgaaatat ttcaacacta ttaaatgaca aaggacgggg tagtgtcata
    32581 ttcatactat cactttgtat aatattcgga atattatata gaaaagaata ggaggattat
    32641 tatgactcaa atgatatata cggtacaacc cggtgacacg ctttatagta ttgctcgcct
    32701 gtatggaagt acaatacaag caattgtaga tgcaaataat attttaaatc caaaccttat
    32761 atatcccggc tctgttatat tgatacctgt tgaagaggaa tacttagaaa ccccgcccgg
    32821 aagccttatt tataccgtac agcccggcga tagcctctat attatttctc ttttattcaa
    32881 ggtaagcatt caaagaattt tagaattaaa cgacattccc gacccttcat tgatttatcc
    32941 gggaatgaaa attatactgc ccccagaagc tcttaatcca tttcaaccta tagtgcccgg
    33001 cataatcaga tataccgtac tgcccgggga tactatatat aaaattgctg caaggtttgg
    33061 aacaacagct cagtctatta taaatgctaa tcccggtctg gaccctcgaa gacttattcc
    33121 gggaacggta attacaatta caattcctga aaatgctgtt gccatataca gaggaaatcc
    33181 aaacagaaga atggttgctt taacatttga tgctacttac ggtgataacc agacttatga
    33241 attgcttgaa atattaagaa acaataatat taaggcaaca tttttcctgt ccggaatctg
    33301 gttgataaat tatcctgatt tagcaagggc tatagcagct gaaggtcatg aaataggaaa
    33361 ccacagcctt acccaccctc atatgccgct tataacaatg caggaagtca caaaccagat
    33421 tgttcgaaca gaagctctta tcaggaatat cacgggtcaa gacccatatt tgttcagacc
    33481 gccttatgga gaatatacgc aagcaattct aaaccaattg gcttcacttg gctatgtaac
    33541 aatcctgtgg acgattgact ccttagattg gcaaaatccg ggagctgctg caatagtatc
    33601 gagagttgta aataatgcag agcccggagc aattatcctg ctgcatcaat cggcacccga
    33661 cacacttgaa gggctgcaat caatgataac acaattaaga cagcaaggat acgatttcgg
    33721 aacagttaca caagtaataa gtccgctata atcacaagag gacggggttg tcataatcat
    33781 taccaaccac gtccgcttac ttttgtaagt gctataagtt tttaacctaa attccagcag
    33841 tgatttaaag gtcctcttcc ttttcctata tttaaattag attttattgc gcctgttata
    33901 tatttttttg catttaatat gcttgtttta acatcattgc cctctgctaa attgcacgct
    33961 atcgccgatg aaagcgtgca gcctgttcca tgtgtattta gattatttat cttgggctgc
    34021 tcataccata taaattcacc atttgaatat aataaatcat cgctgctaag agttaagtgt
    34081 cctcccttga taagaatata gcccgaataa tttttggata tttttaccgc tgctctttgc
    34141 atatcttctt tgctctcaat ttcaatcccg ctaagcacct gagcttcctg tatgttgggt
    34201 gttattacgg ttgctaaagg aatcagctta tttataagca tttctactgc atcgtcctta
    34261 aaaagtttgc ctccgctcgt ggcaaccatt actgggtcaa gcactatttt tcctgctttg
    34321 tattcaatca gcttgtctgc aataacagat ataatttctt tattcgaaac cattccgatt
    34381 tttattgcat cgggataaat atccgtaaaa accgcatcca tctgctgagc aacaaattca
    34441 ggcgatgatt cgaatactcc gtatactcct gttgtatttt gagccgtcag tgctgttata
    34501 acactcatac cgtatatttt gtggctggtc attgttttta agtcagcctg tattcctgct
    34561 cctccgcttg agtcagaacc ggctattgtt aatacctttt tcatattact tcctctattc
    34621 tattattttt tttgctaact ctaaaagttc tgatgcagca gttttaatat cattttgtgc
    34681 aaaaatagcc gatacaacag atattccctc tatgcccgta cctgcaagtt gaagtgcatt
    34741 atctttggat attcctccga ttgcaaccac aggtattttc acggtattac atatttcctt
    34801 taaggtttca aatgataccg tatttgcatc aagttttgtt aaagtattaa aaactgcacc
    34861 cactcctata taatctgcac cgctctgctc ggcttgaaca gcttcttcca ctgtagcggc
    34921 agaaagaccg attattttat cagctcccaa tttttctctg gctgatttta tttcttcgtc
    34981 gtcttggccg atgtgtgctc cgtcagcacc gactctaact gctatatcta cattatcatt
    35041 gattataaat ggaatattgt atttgtcagt aactgcctta acatccatgg cagtttttaa
    35101 aaattcttca tcgcttaaat ttttttccct tagctgaata caagttgctc cccccagtat
    35161 tgcctcttcc acggcttcgg caactgtttt tccctttaac caggctctat cagtaattgc
    35221 atataatagc agtgatttac tgtctaattt caaatttcgc accttcctcc aatattttat
    35281 catcaatttt gctcataaaa tctattaaat gtgaccttag cattgatgtt ccttcatcat
    35341 tctttaaaat tttctcataa gctaattcac cgcttaatcc gatgatacat acagcagctg
    35401 ctgtggcatc gacaatattg tccttgttag caccgcagta tgcaccgatt atagacgaaa
    35461 gcatacaacc tgtaccggat attttaccca tcattggatg tccgtttcga ataatatatg
    35521 ttttcttact gtctgaaaca atatctattg caccggttac ggcaatcacc gaagaaagct
    35581 tttctgataa tgacttaata tattcaacaa cttcatctaa attttcttca ttaattgaat
    35641 cagcaaggtc agcatcgaca cccttggctc cctttgcatt tgtatttcct aatgctttaa
    35701 tttctgatat attgcctctt acaacggcaa acttcacttc attcaataat ttgtttgcag
    35761 tatccgtcct tagtcttgat gctcctactc ccacaggatc aagtataacg ggtatatttt
    35821 gttcatttgc ttttttccct gccttaatca ttgattcaat ggttcttgaa tttaatgtac
    35881 cgatatttat aaccaatgaa ctgcttatgg atacaatttc ttctacttct tctatatcat
    35941 cagccattat tggtgagccc ccgcaagcca ataatatatt tgcacaatcg tttacggtaa
    36001 cataattagt gatattgtgt accaatggag tattattttt aacattttta ataatttctg
    36061 aaaacatatt aacctcctta aataatataa aaaatatacc gcctgcagac agtatattac
    36121 gatcgtttat gttccctacg tcggcattac ccgaatcagg tgcggtcgaa acaatgtttc
    36181 ctctcagcct tacaagctcc cgaattattc agttaattta atatagcatt gatttatcat
    36241 catgtcaaga aacttgaaat atttataaaa gttctttaaa tgattcgata tatatatcgc
    36301 ttattaattc gatatccttt ctggcatttt taaaataatc atcatatacg ccaatagtat
    36361 aaaagcctgc ctccttagct gtaatcaatg caaaatcagc atcttcataa actgcaattt
    36421 cggatggctt tgcatttaat ttatttgcgg caagataaaa tatatccggt ttattctttc
    36481 ctaccccgac ctcgtcgcag cttataataa atgaaaaata ttttatgatt ccatttctct
    36541 taagcgctgc ttcagccaac ttcaaaggtg ttgctgttgc aatacacatt attgaattct
    36601 cattttcaag cttttgaagg tattctttta catagggttt cagtttaaaa tcatacctgt
    36661 atttatcttc aatcatcttt ttaacttctt taattatttc atcagtgctt aaatctattt
    36721 taaaagcttc ttgaaaatat gttgcagtct caatcattga catagcagat ataatttcat
    36781 ataaattatc cggaggatta ataccctttt ttataagata atccttcccc aaatttagcc
    36841 atgcatccat tgaatccaac aatgtcccgt ctaaatcgaa aattttgtat ttaaattgtt
    36901 tcactatgct tctcctaata ttaattttac atcatattgt taaataatcg ataacatctt
    36961 tacaatagta ctgatttcaa ttgttctctt caatattata cagttttaac gcctatttat
    37021 caagttgtgc ttttatcttg tcaaatgtat ttgttttttt caatgccttt ataagtaaaa
    37081 ccgccaccat tgctcccata attgatgaag gtacaaataa aggtatatac ataaatatag
    37141 agcctttagg caatccataa aacattctca taaagggata tgaaagaatt gcacttatta
    37201 taccggtacc gattacctct cctataaccg cccagtacaa tttttgaaac cttttatata
    37261 gtattcctga taaaaatgct cccacaattg ctccgataag cgctaatatt gtacggccgt
    37321 ttattatcat tcttaataat cctattgtca atgcacataa aaatgcccat gcaggtccta
    37381 aaaatacagc tgccgttaca tttatgaagt gctgaaacgg agccatcccc ggtatccata
    37441 taaaagtatt gagaacatat cccatacaca ctaacaatga agcaaaaaca agttttttta
    37501 aattaatttc tttgtttttt atcatttctt cctccttaaa tttgttttaa ttaattatta
    37561 aaaaagcata aaaagaagat tgtacccgcg gtggtgcacc cctttttatg ctttcaacat
    37621 tctgtcaata atattattgt tattattaaa ttatacttta agctttctta ttttatctgc
    37681 tattacacat atgattacgg ttataatcat tacgggtatt gtgcttccaa tagccaaatt
    37741 catgtttaaa agcagtctgt aaatcgtaaa tcctatcagc caaataatga aatttgtcac
    37801 atccacagat ttttttatat tatctttttt aagtataaaa taatctgcta tgagaatcga
    37861 aatcattgga gcaaacactg agcctatcaa atataaaaaa ttctgatatt gctcgacagg
    37921 tgtaaaaatt gcaatggcag tgccaattaa acctactgca atagccatgg ttttttcagg
    37981 caatccggca tgaattgttt tcaagcttac tcctgccgaa taaacatcca aatacgtcgt
    38041 tgttaccgtt gaaaacaata ttattaatac gcctaaaatg ccaaatcctg ccttatacat
    38101 tataaccgct atatcacttt cgccggtaaa gatagcagat cccaaaccaa ttgtatacat
    38161 gaaaacactg cctgcaaagt atgtaatcac acttacccgt gttgcagtta ttggatgctt
    38221 tgcatccctt gtgtaatcag aaataagcgg aagccacgaa ataggcatag ctgcagacag
    38281 ttctatcgct gctccaaaac ccatttcacc ggaaatgttt acattagaac ccctgttaaa
    38341 aacagtagct cctagaaaaa ttgtcagaat aaacaagctt cccactgcaa aaatatttac
    38401 cttctccaag tttttaggtc caataacaac ccatactaat attgcagccc ctataataat
    38461 gccccacaac aaatcagatt gcataccgag atttgtattt aacataaccc ccattgctct
    38521 tgcgccttga attatcatta cggcagtcca tccgacaagc tgtaatacat tcatcacggc
    38581 aaaaaaattt cttcctttgt ttccaaatga taatcctaca gattccatag ctgatttacc
    38641 gcttttagca cctatgattc cggcataata taaaagtatg cagccgatta tgtgtccaat
    38701 cacaatagca ataaatcctt ttgaaaaacc aagcggtaca aataatgttc ccgtaaatat
    38761 ttccgcgata gatacggctg ctccaaacca gagtaacccg ttgcttaacg ttgatgtttt
    38821 gtaactaccc ataacttatt tccttcctat taaaaatggg atataccaac acggtgtatc
    38881 ccattcactc tcataaacag ttccctacgt tggcattatc caaatcaggt acggtcgaaa
    38941 ctttaagttt cctctcagcc tattatgctc ccgatattat taatttaatt aatttgatta
    39001 tacaacaagc ctacagtaaa tgcaatctat ttttcataat ccaatgtttt tgctgttagg
    39061 gtaattttca ttcatgtaac attaaggggg ctcgggtctt tgacacttga agaataagaa
    39121 aagcgataaa gtcattggaa agacttt
//
LOCUS       DAOSHP010000009        35993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_927_length_35993_cov_8.844120, whole genome shotgun sequence.
ACCESSION   DAOSHP010000009 DAOSHP010000000
VERSION     DAOSHP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 35993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35993
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_927_length_35993_cov_8.844120"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..3490
                     /locus_tag="PLB33_01860"
     CDS             83..3490
                     /locus_tag="PLB33_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOG62297.1"
                     /translation="MSLKIIAGRANTGKSSFIYDEIKTQSKNSKAKLILIVPELMTYQ
                     AESNIIERFDLPGIMNIEILSFKRLERKILEEVGGLKMQDLSVFGKIMLLKKIFEENK
                     DKLKVYGNSYKQSGFLKEFEELITEFKQNLVEGQSLVQAAQDMDDEILIRKLSDIQLI
                     YSEFIERTKNKYFDEEDKTNLFISLIKESAYIRNSNIWIDGFESFNRQRLHLIKNLCD
                     YSKSVTLSLNIDSSYLDNLEEKDDFEAFKIIYDTYHTLKNFDTDIEIISLSENKSPST
                     EIKAIEKNIFSLNIEEYENNTDKINIFSSLNPYSETQKTAANIISLVRDENLRWRDIK
                     VAVGNMEVYETNIKRIFSQFEIPFFLDVKRDILNNPLSKYILSILDMFIWNFKHDNVF
                     EYLKTGFSPLNINQVQYLENYALQFGIEGDKWFREFKSFDKDGNQITGEKNNIERYRK
                     LFAEDFEVERKKIHNLKTANEITKLIFKYLDKHKIKEKMEKAVKEFNNAQKYEEASEY
                     SQCWNYVMEIFEQLLLIGQDTEISLNDYRKMLEAGLGEVEVSIIPPAIDKVEVGTIDR
                     IAVTKPKALFIIGANDSNLDTKNTDKGLLLNDERDILLENDVRLTKGADYDTFKEKHM
                     LYKMFSSPSHSLSVSYPLGLESGESLQPSLYIDILKKIFVSVKESSDLSISDELDLVS
                     NYSGTYDYFVEKMRNFIDGYETDNIWKDVYAWYEREEKEIFDIMVKGLNYKNAMNSLE
                     PDIVKRIYENNMSMTVSKLESYAECQFKYFMENVLKPRERAYLKIEYYDLGNIYHSIV
                     EKFINKISEDKEDIIILDRTKAEDEAVKITDDVLLEQADKLTAIDANHRNKYMKEKIK
                     RVMKRTCWTIVRQLNSSEFRPKFTELQIDLVDKNDEKAKKGVYLPPIEIPVETDKIKE
                     VLKLRGKIDRVDVFENNDKLYISIIDYKSSPNDIDLEDAQEGIQVQLIMYLKALLDKG
                     EELFGKKPCIGGVFYYYISDPVYKDKNKEKEPEEEIFKSLKLKGYVLKDKEVIKYMDK
                     NIGSTSSIIPAALKSDGEIRDDRTKALTEESFNNLIDTVYNKCKDMTKSIISGNININ
                     PYKKIDGKTPCKYCEYISICQFDKTLGNNFRLIGNQFKNLSNLYTEH"
     gene            3505..7224
                     /gene="addA"
                     /locus_tag="PLB33_01865"
     CDS             3505..7224
                     /gene="addA"
                     /locus_tag="PLB33_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HOG62298.1"
                     /translation="MNWTKEQKRIIDLKNSNILVSAAAGSGKTAVMVERIISLIKSGE
                     DIDRLLVVTFTKAAAAGMKQKIQKSLLNAYQNKDGNIKHLRKQLSLLNRSMITTIDSF
                     CLDVVKKNFHLIDIDPNFRVGDKSELSILLQDSIDEVLEEEYEKINENENFRKLVEGF
                     TGNRGDDELSEIIQSTYRFILSFPDPFVWLSQSVDKLNIKGEELKNSSWLKEIKSYIM
                     LLLDGAKGYINTALEICNEPKGPRLYLDTLKKDMAIVDNLVNLLNNNMEEFMEAVKGF
                     KAPKAPPFKEKDNPDVDIEKQIEVGGSKNNNSMRAGYKSIIASIKELLPYDLDYDKYA
                     LEINEMYGSIAALRDLIIKTDKIYKEKKNEKSIADFNDLEHFALNILRSKNDKGVFEP
                     SEAAEYYRKKYNYIFIDEYQDSNSLQEAIIEQIKRENNLFMVGDVKQSIYRFRLADPS
                     IFNNKYTTYTMDREDLSNNIVDRTIDLNKNFRSRDEILKAVNFIFKNIMTKELGEVEY
                     NEKAALNTGSEFLTHNPVELNIIYKNTSLENQEGEAEAGEVIKSEAEIEIESMETAEL
                     EALFAAKRIKELIKEETCIPGKTKGKKIEYKDIVILLRSVVNWAGIFEERFNKEDIPF
                     YYDGGKGYYDALEVRILVNLLRLIDNIRQDIPLLSVMRSPIGNFSTEELLEIRLKHPN
                     EKHFIDALNKYILCAKEEEESFPLADKLKNFKEKIYDWSYRSRYSHLHDLIWDILMES
                     DYYNFAGALPNGRMRQANLRMLADLAYDFEKTSMKGLFKFLRYIEKIDKGYDDRGQAK
                     TLGENDNVVRLMSIHKSKGLEFPVVILCGLNKQFNLQDTKNKILLHKDYGIAPMYVNV
                     NERFERETLARTAIKMRIKKENISEEMRVLYVAMTRAIDRLIMAGTVSNLEQKEKNWR
                     RGHSQYFVYKGLSYLDWICSCLFEKINFDYFNEIMAQGQWKNWILNLITRADLSLSIR
                     DEINFKESNIRAMNEYKDMKNSPYFEEIGRRLGFKYPYKASVNVPTKLSVTELKNLKG
                     KEYAKLRYKIPTLVDIFEYNEVTDKLIFNKEITGAEIGTLLHFVMEHLDLKGNLKKSG
                     IISTIKQMENKKLLTEAESKIAISTYAEKIEEFFQSKIGKRIIDSSAVYREAPFVLRK
                     KANDISGSLNKDDLILVQGIIDCYFIEDGEAVIVDYKTDKIDETKEIEFQINNLKSEY
                     KDQIELYKEAVEKITSKNVKECYLYLFSIGKEVKI"
     gene            7314..7964
                     /gene="deoC"
                     /locus_tag="PLB33_01870"
     CDS             7314..7964
                     /gene="deoC"
                     /locus_tag="PLB33_01870"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOG62299.1"
                     /translation="MEYLELMKYVDHTLLKPYATWEEIRVLCDEAINYQTASVCIPPN
                     YVKRVHDTYQHKLNICTVVGFPLGYSTTEAKVAECVQALNDGADEIDMVINISDVKNK
                     DYKKVEDEIRTIKETVGEHILKVIVETCYLTNDEKIEMCHAVTKASADYIKTSTGFGT
                     GGATMEDVQLFKKYIGQNVRIKASGGIRTLNDMEAFINAGCSRIGTSSAIKLINNA"
     gene            8008..9192
                     /locus_tag="PLB33_01875"
     CDS             8008..9192
                     /locus_tag="PLB33_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG62300.1"
                     /translation="MKLWRKLAIVALVFIMIFAFSACANNQPEPPEEPETPETPETPE
                     TPEEPEKPAANVFELALITDVGTIDDKSFNQGAWEGVEAYAKEHNITYKYYQPAEQSD
                     TAYIDSIGLAVKAGAKVIVCPGFLFEVPIHTVQTMYPEVNFVILDGAPHAGDWVPDIA
                     DNTYSIFYAEEEAGFLAGYSIVKEGYTKLGFMGGMAVPAVVRFGYGFVQGAEYAAHEM
                     GLTDPVQIKYTYVGNFDATPENQAKAASWYNEGTEVIFACGGAVGNSVMKAAEAAGTK
                     VIGVDVDQSAESETVITSSMKNLSKSVYDALTAYYAGNFPGGTSVTLDATVEGVQLPM
                     DNSRFETFTQADYDAIYGKITAKEIEIMNDAEVVEDAGKPAEEVTAADIPVSKVQVEV
                     IQ"
     assembly_gap    9199..9298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9350..10885
                     /locus_tag="PLB33_01880"
     CDS             9350..10885
                     /locus_tag="PLB33_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62301.1"
                     /translation="MEYVIEMNNITKIFGNFKALDNVTFNLRKGEVHALLGENGAGKS
                     TLMSVLFGLYQAEKGSIKINNKTVKINNPNDASSLKIGMVHQHFKLVHNFTVLESIIL
                     GRETVNKGILKKDDARKKVMELSERYKLRIDPDAYISDITVGMQQRVEILKMLYSDSE
                     ILIFDEPTAVLTPQEIEELMKIMKELVKEGKSIVFISHKLNEIKEAADRCTVLRKGKY
                     IGTVDVKTTTKEQMSEMMVGRKISFNIEKDDIETGEAVLEVKNLTVKAKNSKKNMVNN
                     VSFKVKAGEILCIAGIDGNGQSELVYGITGLIPINEGQIFFKGKDITKYSVRKKSING
                     MSHIPEDRHKHGVILDFDLKQNAVLQTYFQPRFQKRGFIKFNAVSEYAKKLIKQYDIR
                     SPRDEDSIVRNMSGGNQQKLIVARELDKDPDIVIAVQPTRGLDIGATEYIHKQLIAQR
                     DQGKAVLLVSLELDEVMDVSDRILVMYEGEIVGDLNPKEITVEELGLYMAGSKRSDRY
                     EKN"
     gene            10872..12059
                     /locus_tag="PLB33_01885"
     CDS             10872..12059
                     /locus_tag="PLB33_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62302.1"
                     /translation="MRRTRLLQYEGFTSFLSSLMAIVAGLLVGLIILLISNSEDAFPA
                     FLTILKGGFVDGSRGLAQVIYFAIPIIMTGLSVGFAFKTGLFNIGTPGQFTLGAFVAI
                     YIGIKWTFVPPEIHWLVALLGAMVAGAVWGSGPGILKAFFNVNEVITSIMMNYIGMYL
                     VNMSIVKTVYDSLKNQTKPVLKSAVLPKAGMDKLFNTSNVSIGIFIVILAVIVIYIIL
                     QKTTFGYELKACGSNKDASKYAGINEKRSIVLSMVIAGALSGLGGGLLYLSGSGKYIH
                     VLDVLAPEGFSGISVSLLGMSHPIGIFLAGLFIAHLEVGGFNIQLYNFVPEVIDMIIA
                     VIIYCGAFALLFKQIISKFIIHDTDEDIGKSVQAADIPASYESSVVNAETEEKTEQRE
                     DKK"
     gene            12056..13009
                     /locus_tag="PLB33_01890"
     CDS             12056..13009
                     /locus_tag="PLB33_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62303.1"
                     /translation="MSTVYFLAQQTMYFAIPLLIVALGGMFSERSGIVNIALEGIMVV
                     GAFFGIGFIHIFQDSMSGQSLLLLAVLVSALTGAIFSLFHAFASINLKANQVISGTAL
                     NMFAPAFAIYTARMIQGVQQVQFMDTFYIRSVPILGDIPILGPIMFKNAYITTYIGLL
                     IALIAWFIINHTRFGLRLRACGEHPQAADSVGINVYHIRYAGVIISGILAGIGGLTFV
                     VPTSTNFNATVAGYGFLALAVLIFGQWRTKKIFVAAFFFGIMKTFASAYSGIPFLKGL
                     PISNEVYKMIPYIATLIVLTFSSKKSQAPRAEGIPYDKGSR"
     gene            13026..14537
                     /locus_tag="PLB33_01895"
     CDS             13026..14537
                     /locus_tag="PLB33_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOG62304.1"
                     /translation="MFADNKIWIGKSDKKVYLEPKMANRHGLVSGATGTGKTITLKVL
                     AESFSDLGVPVFIADIKGDLASIAIPGTDNENMQERLNRLEINNFIYKGYPVQFWDVY
                     GEGGIPVRTTVTEMGPLMLSRILNLNDTQTGVLNIVFRVADERGLLLLDLKDLRAMVQ
                     YVGDNSKDFRMKYGNITAQSIGAILRSLMTLEDQGGEFFFGEPELDIFDWIKTSADGR
                     GYINVLHCAKLFLNPLLYSTFLLWMLSELYENLPEEGDLEKPKIVFFFDEAHLLFEDA
                     PKQLLDKIELVVRLIRSKGVGVYFVTQNPIDVPNDILGQLGNRIQHALRAYTPAERKK
                     VVTAAETFRENPEFNTVEAITELGTGEALISCLDEKGIPSVVERAFILPPQSRFGTID
                     DMLRNQIIAGTPIYKKYYKSVDRESAYEILLAKTERKYQEELLETERLEEEKRLKLEA
                     KERERQQKEFERARKRASSKTTAIDRVANSAMNAIGREVGRSLIRGVLGTFKR"
     gene            14537..14881
                     /locus_tag="PLB33_01900"
     CDS             14537..14881
                     /locus_tag="PLB33_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOG62305.1"
                     /translation="MYNKNKGFEYEKIAEKYLQNNNYKILEVNFISKFGEIDIIAEKD
                     NRLHFIEVKGRKNIKHGYPKEAVTFSKQKRLRSAAKYYFMLKGKDDYLCQFDVIEIIL
                     ESRDINHIKNAF"
     assembly_gap    14928..15027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15099..16412
                     /gene="mtaB"
                     /locus_tag="PLB33_01905"
     CDS             15099..16412
                     /gene="mtaB"
                     /locus_tag="PLB33_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOG62306.1"
                     /translation="MNKDTSKVYTVSFITLGCKVNQFETEAMTEILERDGFKVLPPNE
                     VSDIYIINTCAVTKESERKSRQFINKAKRINPDALVVAVGCSVQLNSEKIKKETQADI
                     IIGTKHKGNIGKLIKNKLNDIKQECKIDDFRGRESFEELKISNVYDKTRVNIKIQDGC
                     SQFCSYCIIPYLRGPIRSRNHEDIIGEVKRITKNGYKEIVLNGIHISSYGKELKENYY
                     LIDLIEDINKIEGIERIRLGSIEPNLINEDFMKRYAVLDKACDHFHLSLQSGSDSVLK
                     RMNRKYSTNEYKNNVELIRNYMPDAGITTDIIVGFPGETDEEFNETLEFVKKIKFSRI
                     HVFKYSVREGTKAAEIKPQVNEQVKADRSKVLIELGKNISKEFMKKFIGRKLSVLIET
                     KKNENIYEGYTTNYLKVMLKSDINVKNEIINIHVTGIRNDYLLGE"
     gene            16435..16776
                     /locus_tag="PLB33_01910"
     CDS             16435..16776
                     /locus_tag="PLB33_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOG62307.1"
                     /translation="MDCIFCKIANKEIPSQFVFENEYVVAFRDLAPQAPVHILAVPKI
                     HITSMNEITKENSHIVAEIFEAINKITESEGIRDSGYRVISNCGRDGCQSVQHLHFHI
                     LGGKKLSESLA"
     gene            16884..17069
                     /gene="rpsU"
                     /locus_tag="PLB33_01915"
     CDS             16884..17069
                     /gene="rpsU"
                     /locus_tag="PLB33_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOG62308.1"
                     /translation="MTEVKVRENESIDNALRRFKRQCALTGVMSEVRKREHYEKPSVK
                     RKKKAEAARRKKNKKAR"
     gene            17081..17524
                     /locus_tag="PLB33_01920"
     CDS             17081..17524
                     /locus_tag="PLB33_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOG62309.1"
                     /translation="MPLKEKLMEDLKESMKSKNKVKKDTVTMVRAAIKQKEVDERIEL
                     SDAEIIDIIAKQVKQKKDSIPDFEKGNRQDLVDLTNEEIKILLEYLPPQLSDEDLESI
                     VKEAIEKTGAQSKKDMGKLMAFIMPKVKGKADGKHVNEIVAKYIK"
     gene            17688..17969
                     /locus_tag="PLB33_01925"
     CDS             17688..17969
                     /locus_tag="PLB33_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HOG62310.1"
                     /translation="MSKFNNFRSKLADELEIPNNALSDNFELRMHGNKKVIIENHLGI
                     KVYDENMVCIKTNDQDITIKGSKFKIEEINNYKVIVKGNIKEIIFSMKE"
     gene            17972..19177
                     /locus_tag="PLB33_01930"
     CDS             17972..19177
                     /locus_tag="PLB33_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOG62311.1"
                     /translation="MLIFKLMYLIRGYVIIRLDAKNSEKLLNILQRRNIKVWDVEKKG
                     KGIKFKISYEDYLKNSTLINDIVKPESKRGFLLKLNKVKLRKGFSIGLLILIITLYLL
                     TSMVWDVEVIGTNQSNTNEILALLEKNHIKIPVSISRIEAKQLETLIHKNFKYKFVDV
                     FVEGSKLIIFVREREPDPAEIKSNEPSSIISKKNAIINKVIAKSGQPVVKEGDVVYEG
                     QTLIMGIVKNKNSDEFMMVPSDGIIYGKTYYNFEMKEEKIKSMNVPTNKSKIVYYLKI
                     NENSIKILGNKDPYQNYEYRENEIKVPILSNIADIGFIKGVYYEEVIKEIEIDKNTAQ
                     NKMKIAMYDELLKRCDDDSRILKSNINFSEDEKFYYLIAQIEVIEDIGERVRIFSTEE
                     EKTDETKED"
     gene            19177..20136
                     /locus_tag="PLB33_01935"
     CDS             19177..20136
                     /locus_tag="PLB33_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOG62312.1"
                     /translation="MELFEQNVLIANEDELIKLLGIHDKNIKIIKSYYDVNILVRNGA
                     LKITGEKENSEAVSKIIKDILNTIRTEGDISDQKVIYLIEANKSNSQIDYNQILKEVI
                     VTSASGRIIKPKTVGQKRYVDMINSKDVTFGIGPAGTGKTYLAVACAVNAFRKKEIER
                     IILTRPAVEAGEKLGFLPGDLQDKVDPYLRPLYDALFDIMGIDNFMKNVEKQLIEVAP
                     LAYMRGRTLDSSFIILDEAQNTTNEQMKMFLTRLGYGSKAVITGDITQIDLPEGKKSG
                     LKSVINILKDVEDIGFMFFHTTDVVRHKLVQDIVKAYEKYEVK"
     gene            20136..20582
                     /gene="ybeY"
                     /locus_tag="PLB33_01940"
     CDS             20136..20582
                     /gene="ybeY"
                     /locus_tag="PLB33_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOG62313.1"
                     /translation="MIKVFFDDRQDVMEITKDNEDAIKNAVDAVLTAEGLNGDFEVSV
                     SFVTNEEITELNREYRNVDSETDVLSFPMNEEFDGVNILGDIVISTQKIIEQANDFNH
                     SMEREMCYLTVHSMLHLLGYDHMNKEEKNKMRAKEKEIMKKLKIFK"
     gene            20602..21666
                     /locus_tag="PLB33_01945"
     CDS             20602..21666
                     /locus_tag="PLB33_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOG62314.1"
                     /translation="MKTKIAVLIICLLFINACAKETVTPEQAEPEINEEEPEEKPEVD
                     KEEIEEEIDPNKVLSPLDGLRYYPEELSKRPVAVSIDNHHRARWQAGISQAEIVYEVE
                     VEHPFTRYLCIFLAHEPEMIGPVRSARPYMIYYALENDGIFVHVGGSEDAFSEIKRLK
                     VADVDGLYSGAMWRYNDTGKYAPHNVYTTLESIRNEAKAYGYRDEGNFEGYLFNEKDV
                     KLSENFETTPSKEVNIVYNYYNTTDYIFNEENNLYLRFKDDEEHIDELDKKQLTAKNI
                     IAVETSKSILDNEGRLFLGTIGKGKGIYITNGESIPIIWEKTSEKDRLKFYSGDEELM
                     LNPGNTWIQVVNSLENITLR"
     gene            21708..22100
                     /gene="cdd"
                     /locus_tag="PLB33_01950"
     CDS             21708..22100
                     /gene="cdd"
                     /locus_tag="PLB33_01950"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOG62315.1"
                     /translation="MEDKLLIKIAMEAREASYSPYSNYKVGAAVYTKSGKVFTGCNIE
                     SASFTPTICAERVAVSKCISEGYRDIYKIAVVGSEIKTSFPCGVCRQFLAEFGKDIKV
                     ICARNIDSYDVYTLEELLPNSFGPDDLA"
     gene            22113..23000
                     /gene="era"
                     /locus_tag="PLB33_01955"
     CDS             22113..23000
                     /gene="era"
                     /locus_tag="PLB33_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOG62316.1"
                     /translation="MFKSGFVTIIGRPNVGKSTLMNNMLGEKISIMSDKPQTTRNKIR
                     TVYTDDEAQIIFIDTPGIHKPKNQLGEFMNTEVDSALEEMDVLIMITDEFNKVGPGDE
                     FIIEKIKDIKAKKILIINKIDKFDKEAALRIAAEFDKYKVFDDIVPISALNNVGVDAL
                     VKLIIKYLKPGPMYFPADYITDRPERFIVSEIIREKALMYLEDEVPHGIAVEIEEMKE
                     RKDRDLVDIRANILCEKKSHKSIIIGKEGRKIKGIGKSAREEIERLLGSQVNLQLFVK
                     ISENWRDDSYRLKTLGYDK"
     gene            22997..23710
                     /gene="recO"
                     /locus_tag="PLB33_01960"
     CDS             22997..23710
                     /gene="recO"
                     /locus_tag="PLB33_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOG62317.1"
                     /translation="MIQEEILILKEISYKENDKILHALSRTRGKIRIISKGSKKSSSH
                     LINASQIFAYSTCTLNVSKDMYILTSAKLLDNFYNLNKNMAAYYNASYILELISYVAQ
                     ENEVDSRVFDMTVSVISHLSNCTYEHEKLTAAYELKLAAMLGYKPDFLHCLRCGSSIN
                     DESKFSVTEGGVFCSKCVNFGNGINVTYNEILIMDKILKSKFENIGFIEIPKTIMNLI
                     RKFLFYYIGKDNFTTLKLL"
     gene            23727..24329
                     /locus_tag="PLB33_01965"
     CDS             23727..24329
                     /locus_tag="PLB33_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOG62318.1"
                     /translation="MENDTLNKIDEIIKRTNTDYGTAKEALEAANGDVLEAIILIENQ
                     QKGSTGSFRGEQIMAQLKDILAKGNATKLTIKKDNEVIVNIPITAGLVSAFIAPFLSA
                     AGITVALLAKCSVEITQTDGKIIDLGQKVDEGIDAVKDAMHDIKKGAQNLKDDLRTGS
                     KNIKDDIKTGAENVKDNIKAEAENMKDDLKNSFDRNNVHF"
     misc_binding    24373..24550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24656..26050
                     /locus_tag="PLB33_01970"
     CDS             24656..26050
                     /locus_tag="PLB33_01970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOG62319.1"
                     /translation="MAKIEKNSLEKVVSLCKSRGIIFPGSEIYGGLANTWDYGPLGVE
                     IKNNIKRAWWKKFIQESPYNVGLDAAILMNPTTWVASGHVGGFSDPLMDCKECKERFR
                     ADKLIEDFMKEIDDVQVVDGWTNDEMEAYVAEKKVKCPNCGAHNFTTIRKFNLMFKTF
                     QGVTEDSQSELYLRPETAQGIFVNFKNVQRSMRKKIPFGIGQIGKSFRNEITPGNFTF
                     RTREFEQMELEFFCKPGEDMEWFYYWKEFCMNWLLNLGMNKEKLRFRDHEPAELSHYS
                     KGTTDIEYLFHFGWGELWGIANRTDFDLKQHINTSGEDLYYQDPITNEKYIPYCVEPS
                     LGADRVMLAILVDAYNEETLEDGTDRVVLKLHPALSPYKAAIFPLTKKLNEQAIELYQ
                     MLNKHFNVDYDDAGSIGKRYRRHDEIGTPYCITFDFDSLEDNTVTVRDRDTMEQVRIK
                     IDDLVTFIDEKTSF"
     gene            26117..27118
                     /locus_tag="PLB33_01975"
     CDS             26117..27118
                     /locus_tag="PLB33_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOG62320.1"
                     /translation="MNIREKYEQFEIEHLSEYASKAALSKGRMLYEPKCEIRTDFQRD
                     RDRILHSKAFRRLKHKTQVFLSPEGDHYRTRLTHTLEVSQISRTIARSLNLNEDLTEA
                     IALGHDLGHTPFGHTGERILKELCNGFKHNEQSLRVVDFLEVRNERQGLNLTFEVRDG
                     ILNHPSGYKPATLEGQIVSISDRIAYINHDIDDAVRGHIITKIPDKFLKNLGSSHGQR
                     INTMIMDIIQNSENKNKIVMSDEVAELTDELRNFLFENVYYNKVAKSEEDKTMFIVEK
                     IFEYYTNNFEQLPEFYINIYNNNNFSKGEIIKDYIAGMTDRYAMKVFEELYIPKPWI"
     gene            27250..29004
                     /gene="dnaG"
                     /locus_tag="PLB33_01980"
     CDS             27250..29004
                     /gene="dnaG"
                     /locus_tag="PLB33_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOG62321.1"
                     /translation="MPYRLDSDVIQRILEQNNIVDIIEEYLPLKKAGANYSTTCPFHK
                     EKTPSFIVSPDKQIFHCFGCGESGDSISFLTKYKNYTFVEAAEYLANRAGIILEEINS
                     SRINNNKQTADMLYKINRDAAAYFYKNLRKSPNAIKYLFSRNIKSDVIKSFGIGYAID
                     EWDNLLNYLKNEGYNEEDILKTGLIIWNDKKNTYFDRFRNRIIFPIFDIKKRIIGFGG
                     RVIDDSMPKYLNSPESLIFNKGYNLYGIHIAKENIRNNSFILVEGYMDVIKMHAHGYK
                     TAVAALGTSFTENQAKLLKRYSRDFYIAFDSDEAGQKASLKAINMLKRNNLNAKIVIM
                     KDAKDPDEYLSKYGKPAFDKLIENSLDYYSFLEFHFKEILENSNKVEYINNLFDNIVN
                     VNSEIERELIFDKLSRKVGVSKESIIKEFNKRSTKQKTLDKTAIPAVKPQVAKITTCH
                     EEELIKLILLDNDFALKLSEIVNEDTFKDLKYYNILKELYECRINDNSIDSKSLNKII
                     KDDMNVNLQFDNVDYDNLEALFQDCMKRLKIKYYKEKRSILNNILKQSDNSLNRKEIM
                     NEIYCLAKKIKSTKEEVN"
     gene            29007..30176
                     /gene="rpoD"
                     /locus_tag="PLB33_01985"
     CDS             29007..30176
                     /gene="rpoD"
                     /locus_tag="PLB33_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOG62322.1"
                     /translation="MSENKNISVISEEEYAKNEEMLQKIDSIKNKLIEKGKRNGFITY
                     KEVLRSFEKLDINPEFIDDFYKQAEDNDLEILGFQDDMIPEKVDEEDEIDSKEDADFF
                     VSQSDDDILKGVNIDDPVRMYLKEIGKVSLLSAQEEVELAKRMEAGDEEAKKQLAEAN
                     LRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGFKFSTYATWWIRQAITR
                     AIADQARTIRIPVHMVETINKLIRIKRQLLQELGRDPTPEEIALEMEMEPEKVREILK
                     IAQEPVSLETPIGEEEDSHLGDFIPDDEVQAPSDVATFTLLREQLSNVLHTLTDREQK
                     VLRLRFGLDDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLKDYLE"
     gene            30438..31112
                     /locus_tag="PLB33_01990"
     CDS             30438..31112
                     /locus_tag="PLB33_01990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG62323.1"
                     /translation="MNRLECIVSMVDKCNTAADIGTDHGYVAEMLLKKGICNKIIATD
                     LNKGPLYRAREHLSEVKLSDKCDFRLGDGLTVLNEGEADTIIVAGMGGDLISEIIERS
                     KHIAESAKQLILQPMTKVDNLRRYLCNNGFEIIDENIVKEYNHFYFVIKTKKGFTKYM
                     DDIYYEISKVLTDKRDTVMIEYINKVIDTNKKIISSLEKADNVAYNEKIEELKRKNKK
                     IRELIR"
     gene            31109..31882
                     /locus_tag="PLB33_01995"
     CDS             31109..31882
                     /locus_tag="PLB33_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOG62324.1"
                     /translation="MNLNNIINLLNEEVKLKEHESWDNSGLQLGSMEVNINKIMLTLD
                     INKEAIEYAVQHKVDLIITHHPFFFGSIRKIDFDTYDGEIIKMLIKENINVYSMHTSY
                     DMADKGVTYSLAKRLNILNYQVLHPINIDNSGYGGIGDVAPVNIIEYTKLVKEALGAE
                     YVKLYCNGEKTVRRVAFCGGSGGEFIEDAIRQKADLYITGDIKYHQAQEAVKNNLCII
                     DAGHYNTEIYSMETLMEVLEKTGLEIINFKKNITAEKII"
     gene            31891..33819
                     /locus_tag="PLB33_02000"
     CDS             31891..33819
                     /locus_tag="PLB33_02000"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOG62325.1"
                     /translation="MDKNFEYIRVASAVPSIRVADPNYNAIKIKEIILKAEKEKVRVL
                     VFPELCLTGYTCQDLFSQNTLINKCEEALSLLLEETKYLNMLIAVGMPVKADTKLFNC
                     AVIFKEGKILGVVPKTYIPNYKEFYEMRWFVPASYRLSDIVTLCEQEVIFSENLLFKD
                     DISSLCVGVDICEDLWATIPPSSYHALQGANLILNLSASSETSGKADYRRDLVKMQSS
                     KCITSYVYTSSGEEESTTDVVFSGHCIIAEDGKILKEDNLCDGFIYSDIDIEKINNEK
                     RSLNVYNSNIERKNYKYVNFNLGYSENLKLERFVEPRPFIPARPEDRDKSFSNILKIQ
                     AAGLSQRMKKIGSKKAILGISGGLDSTLALIVAVEAIKKLKLPLTNIITVIMPGFGTS
                     ERTYKNAHILIDELGVTLREISIKDACMQHYKDIGHDINNHDVTYENSQARERTQILM
                     DIANQEGGLVVGTGDLSELALGWCTYNGDHMSMYSVNSGVPKTLIKYMVKWYADSKTS
                     GEMQKTLLDICNTPVSPELLPPDKEGNIRQLTENSVGSYELNDFFLYNMMKNGYGPEK
                     MYYLAKIAFKEEYDEQVILETLKNFYKRFFTQQFKRSCMPDGVKVGSVSLSPRGDWKM
                     PSDASYELWMKICENIKK"
     gene            33875..34292
                     /gene="rnpB"
                     /locus_tag="PLB33_02005"
     ncRNA           33875..34292
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB33_02005"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(34624..35847)
                     /locus_tag="PLB33_02010"
     CDS             complement(34624..35847)
                     /locus_tag="PLB33_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG62326.1"
                     /translation="MKLHIYSLIMFLNSYLTGLLTPVLSLALIDKGASLSNLSIILGL
                     YALSVILLELPSGIMADVFGRKKTFVLSLVLFTLSFSLLLISKVFIVLCIVMILYGLG
                     RALGSGSFDALFIDYYIDNFGKDKLHNITTRLSVLDALGLSAGALSGGFFPDISNNLL
                     PSIGTYDLNIIVRIVLTIAVIIISVLFISEKNHHDKKEHKSIKQHVKNSSDFVRKNTT
                     ILFIFISVFSTGIFLSALETYWQPHFITLLPDEGSSGLLGLMAFLYLGAALVGSILSN
                     KIMSKYKFNIRKMYLIMRSLLALFLILTALQTNIPLFIAYYASIYLMFGMANIPEDVI
                     LNSETPNQIRASVLSVKSFTIQIGGLSGSLMYSILIRFISIPAIWVIAASIILLTIAL
                     IAKKFIAAEKARNKI"
BASE COUNT        13678 a         4832 c         7195 g        10088 t
ORIGIN      
        1 ccccaatcgg aatttaattg ccataaacga aaaatccttg tataattaag aaaatacatt
       61 tccgtatata gaggcatagg atatgagttt aaaaataata gcaggaagag caaacacggg
      121 aaaatcatca ttcatttatg atgaaattaa aacacaatca aaaaattcta aagccaagtt
      181 aatacttatt gtgcctgaac ttatgaccta tcaagctgaa agcaacatta ttgagagatt
      241 tgatttgccc ggcattatga atattgagat tttaagcttt aaaagacttg agaggaagat
      301 acttgaagaa gttggaggcc tgaaaatgca ggatttaagt gttttcggca aaatcatgct
      361 tctaaaaaaa atatttgaag aaaataagga caaacttaaa gtttatggca attcatacaa
      421 gcaaagcgga tttttaaaag aatttgaaga attgataact gaattcaaac aaaacttagt
      481 tgaaggccag tcattagttc aagcagctca agatatggac gatgaaattc taatacgaaa
      541 attatcggat attcaattga tttattctga atttatagaa agaaccaaaa ataagtattt
      601 tgatgaagaa gataaaacaa atctttttat ttctctgatt aaagaatcag catatataag
      661 aaactctaat atatggattg acggtttcga aagttttaac cgccaaagac ttcatttaat
      721 taagaacctt tgtgattatt caaaatccgt aacattatca ttaaatatcg attcttctta
      781 tttagataat ttagaggaaa aggacgattt tgaagccttt aaaattattt atgatacata
      841 ccatacttta aaaaattttg atactgatat agaaattata tccctaagcg aaaacaaatc
      901 accttcaact gaaataaaag caattgaaaa aaacatattt tctttaaata tcgaagaata
      961 tgaaaataat acagataaaa taaatatttt ctcttcttta aatccatatt cggaaacaca
     1021 aaaaacagca gctaatatta tatccttggt tcgggacgaa aatttaagat ggagagatat
     1081 aaaggttgca gttgggaata tggaagttta cgaaacaaat ataaaaagaa ttttttctca
     1141 atttgaaata ccatttttct tggatgtaaa aagagatatt ttaaacaacc ctttaagcaa
     1201 gtatatactt tcaattcttg atatgtttat ttggaacttt aaacatgaca atgtttttga
     1261 atatttaaaa acaggatttt cacctttaaa cattaaccaa gttcaatatt tggaaaatta
     1321 tgcgctgcag tttggtattg agggagataa atggtttaga gaatttaagt cttttgataa
     1381 ggatggcaat caaattactg gtgaaaagaa taacattgag cggtacagaa aattatttgc
     1441 cgaagatttt gaagttgaaa gaaaaaaaat tcataatctt aaaactgcca atgaaattac
     1501 aaaactaata ttcaaatatt tagacaaaca caagataaaa gaaaaaatgg agaaggctgt
     1561 caaagaattt aataatgctc aaaaatatga ggaagcaagc gaatattctc agtgctggaa
     1621 ctatgtaatg gaaatttttg agcagctttt attgattggg caggataccg aaatttcttt
     1681 aaatgactat agaaaaatgt tggaagcagg acttggagaa gttgaggtaa gtataattcc
     1741 gcctgctatc gacaaggttg aagtgggaac aattgacaga attgccgtta caaagccaaa
     1801 ggcgttgttt attattgggg caaacgacag caatcttgat actaagaata cagataaggg
     1861 gttattatta aacgatgaaa gagatatcct tcttgaaaat gatgtaagac ttacaaaagg
     1921 agcagattat gacaccttta aagaaaaaca tatgctttac aagatgtttt caagtccttc
     1981 ccatagttta tccgtcagtt atcctctcgg cttagaaagc ggagaaagtt tgcagccatc
     2041 attatatatc gatatattaa agaaaatctt tgtttctgtc aaagaaagct cggatttaag
     2101 catttccgat gaattggatt tggtgtcaaa ctacagtgga acctatgatt attttgtaga
     2161 aaaaatgagg aattttatag atggttatga aacggataat atttggaagg atgtttatgc
     2221 atggtatgaa agggaagaaa aagaaatttt cgatattatg gttaagggtt taaattataa
     2281 aaatgccatg aattctttgg agcctgatat tgtaaaaaga atttatgaaa ataatatgtc
     2341 tatgacggtt tctaaattag aaagctatgc tgaatgtcaa tttaaatact ttatggaaaa
     2401 tgtgctaaaa ccgagagaaa gagcttatct aaaaatagaa tattatgatt taggcaatat
     2461 ttatcattca atagtggaga agtttattaa caaaataagt gaagataaag aggatattat
     2521 tatacttgac aggacaaaag cagaggatga agccgtaaaa ataaccgatg atgttttgtt
     2581 ggagcaagct gataagttaa cagcaattga tgcaaatcac agaaacaagt atatgaagga
     2641 aaaaataaaa agagtgatga aaaggacatg ctggacaatt gtgcggcaat tgaattcgag
     2701 tgaattcagg ccaaaattta ctgagcttca aattgatttg gtagataaga atgatgaaaa
     2761 agcaaaaaag ggagtttatt tacctcctat agaaatacct gtggaaacgg ataaaattaa
     2821 ggaagtgtta aaacttaggg gtaaaataga cagagtagat gtttttgaaa ataacgacaa
     2881 attatatatt agtattattg attacaaatc ttctcccaat gatatagact tagaagatgc
     2941 tcaagaagga attcaagtac aattaattat gtatttaaaa gcattgcttg ataaaggcga
     3001 agaactgttc ggtaaaaagc cttgtatagg aggtgtgttt tattattata tcagcgaccc
     3061 tgtatacaag gataagaata aagaaaaaga gcctgaagaa gaaatattca agagcttaaa
     3121 acttaaagga tatgttttaa aagacaaaga agttatcaag tatatggata aaaatatagg
     3181 cagtacttca agtatcatac cggcggcttt aaaaagcgat ggagaaattc gcgatgaccg
     3241 gacaaaagct ttgacggaag agtcctttaa taatctaatc gatactgttt ataacaaatg
     3301 taaagatatg actaagagta ttatatcagg aaatataaat ataaatcctt acaaaaaaat
     3361 tgacggtaaa actccgtgta aatactgtga atatataagt atatgccagt ttgacaaaac
     3421 attagggaat aacttccgtt taatcggtaa tcaattcaaa aatttgagca atctttatac
     3481 tgagcactaa ggaggggcta agagatgaat tggacaaaag agcaaaagcg tataattgat
     3541 ttaaaaaatt ctaatatatt agtttctgcc gcagccggtt cagggaagac tgcagtaatg
     3601 gttgaaagaa ttatttcgct tataaaaagc ggagaagata tagacaggct tttggtagtt
     3661 acctttacta aagcggctgc tgcagggatg aagcaaaaaa ttcaaaaatc attattgaat
     3721 gcttatcaaa acaaggatgg gaatataaag catctaagaa aacagttatc cctcttaaac
     3781 agatctatga ttactacaat cgattctttt tgcctggatg ttgtgaagaa aaacttccat
     3841 ttaattgata ttgacccaaa cttcagggtt ggcgacaaaa gcgaattaag tatacttttg
     3901 caggattcaa tagatgaagt tttggaagaa gaatatgaaa agattaatga aaatgagaat
     3961 ttcagaaaat tagttgaagg atttaccgga aacaggggtg atgatgagct tagtgaaata
     4021 attcagagta catacagatt tattctaagc ttcccagacc cctttgtatg gctttcacaa
     4081 agcgttgata agcttaatat taaaggagag gaactgaaaa actcaagttg gcttaaagaa
     4141 attaaaagtt atattatgct tttattggac ggtgcaaagg gttatatcaa tacagctctt
     4201 gaaatttgca atgagcctaa gggtccaaga ctatatttgg atacattaaa aaaggatatg
     4261 gctatagttg ataaccttgt aaatcttctt aataataata tggaagagtt catggaagcc
     4321 gtaaaaggat ttaaagcacc aaaagctccg ccttttaaag aaaaagataa tcctgatgta
     4381 gatattgaaa agcaaattga agtgggcggt tctaaaaaca ataacagtat gagagccggg
     4441 tataaaagta taattgcttc aataaaggaa ttgttgcctt atgatttgga ttatgacaag
     4501 tatgccttgg aaattaatga aatgtacggc tcaattgccg ctttgagaga tttaataata
     4561 aaaactgata aaatttacaa ggaaaaaaag aatgaaaaat ccatagcaga tttcaatgat
     4621 ttggagcatt ttgcattaaa tatattaaga agtaaaaatg acaagggagt atttgagcct
     4681 tcggaagcag ccgaatatta caggaaaaag tataattata tatttattga tgaatatcag
     4741 gattcaaaca gtttgcagga agcaatcatc gagcaaatca aaagagaaaa taatttgttc
     4801 atggtaggtg atgttaaaca gagtatatac agattcagat tggcagaccc gagtatattc
     4861 aataataaat atacaaccta caccatggac agggaagatt taagcaataa tattgtagac
     4921 agaacaatcg atttaaacaa aaatttcaga agcagagatg aaattttaaa agctgttaat
     4981 ttcattttca aaaatattat gacaaaggaa ttaggggaag tagaatataa tgagaaggca
     5041 gctttaaaca cgggaagtga gtttttaacc cataatcctg ttgaacttaa tattatttat
     5101 aagaatactt ctttggaaaa ccaagaggga gaagctgaag ccggtgaagt gataaaaagt
     5161 gaagcagaaa tcgaaattga atctatggaa acagctgaat tggaagcttt gtttgcagca
     5221 aagagaataa aggaattaat aaaagaagaa acatgtattc cgggaaaaac aaagggtaaa
     5281 aaaatagaat acaaggatat tgtaatttta ttaaggtctg tagtaaattg ggcaggcata
     5341 tttgaagaaa ggtttaacaa agaagatatt cccttttact atgatggagg aaagggatat
     5401 tatgatgctt tagaggttag aattcttgta aatttactta gattaattga taatataaga
     5461 caggatatcc cgctgttaag cgttatgagg tcacctatcg ggaatttttc tactgaggaa
     5521 ttattggaaa taagacttaa acatcctaat gaaaaacatt tcatagatgc tcttaataaa
     5581 tatattctat gcgcaaaaga agaagaggag tcttttccct tagctgataa attaaaaaat
     5641 tttaaagaaa aaatatatga ttggagttac agaagcaggt attctcatct tcatgattta
     5701 atatgggata ttttgatgga atctgattat tataattttg cgggagctct tccaaatgga
     5761 agaatgaggc aggcaaattt gagaatgctg gctgatttag cttatgattt tgaaaaaaca
     5821 tcaatgaaag gtttgtttaa attccttaga tatattgaaa aaattgataa gggttacgat
     5881 gacaggggac aggctaaaac attgggagaa aacgacaatg tggttaggct tatgtcaatt
     5941 cacaaatcta aaggattgga attcccggta gtaattcttt gcgggcttaa caagcagttt
     6001 aatctgcagg atacaaaaaa taaaatattg ttgcataagg attacggaat tgctccaatg
     6061 tatgtaaatg tcaatgaaag atttgaaaga gaaacattgg caagaactgc cattaaaatg
     6121 agaataaaaa aggaaaatat ttcagaagaa atgagagttc tgtatgttgc aatgacaagg
     6181 gctatagata ggttaataat ggcaggaacc gtcagcaatt tggagcaaaa agaaaaaaat
     6241 tggagaagag gccactcaca atattttgtt tataaaggcc tttcctatct ggattggatt
     6301 tgcagctgtt tatttgaaaa aataaatttt gattatttta atgaaattat ggcacagggt
     6361 cagtggaaaa actggatttt aaatcttatt acacgggcag atttaagttt aagtataagg
     6421 gatgaaatca attttaagga aagtaatatt agggctatga atgagtataa agatatgaaa
     6481 aacagtcctt attttgaaga aataggaagg cgtttgggat ttaaatatcc atataaggct
     6541 tcggtaaatg tgcctacaaa gctttccgta accgagctta aaaatttgaa gggtaaagaa
     6601 tatgcgaaat taagatataa aattccaacc ttggttgata tattcgagta caatgaagtt
     6661 acagataaat taatattcaa taaagaaatt acaggtgcag aaataggaac actccttcat
     6721 tttgtaatgg agcatcttga tttaaaaggc aatttgaaaa aatccggcat aataagcaca
     6781 ataaaacaaa tggaaaataa aaagcttctt acagaagctg aatcaaaaat agccattagt
     6841 acatatgcag aaaaaatcga agaattcttc caaagcaaaa taggaaaaag aataattgac
     6901 tcttcagcgg tttacaggga ggcacccttt gtattaagaa agaaagccaa tgatatatca
     6961 ggaagtttaa acaaagatga tttaatactt gtacaaggta taattgactg ttatttcatt
     7021 gaggatggag aagcagtaat agttgattat aaaacagata aaatagacga aacaaaagaa
     7081 attgaatttc aaatcaacaa tcttaaaagt gaatataaag accaaattga attatacaag
     7141 gaggcagtag aaaaaatcac aagcaaaaac gtaaaagaat gctatttata cttattctca
     7201 atcggaaaag aggttaaaat ataacattct tcgcccctgc acctgttaat ctatgtttga
     7261 cgaacctatt attttgtgct agacttattt tataaaaagt attggaggta tcaatggaat
     7321 atcttgaatt gatgaaatat gttgaccata ctttattaaa accgtatgca acatgggaag
     7381 aaattcgtgt tttgtgtgat gaagcaatta attatcaaac agcttcagta tgtattcctc
     7441 ccaattatgt taaaagagta catgatacat atcaacacaa attaaatatt tgcactgtag
     7501 ttggttttcc cttaggttat tctactactg aagcaaaggt tgcagaatgt gtacaagctc
     7561 ttaatgacgg tgctgatgaa atagatatgg ttataaatat atctgatgta aaaaacaaag
     7621 attataagaa agtagaagat gaaatccgca caattaaaga aacagtcggg gaacacattt
     7681 taaaggtaat tgttgaaaca tgttatttga ccaatgacga aaaaattgaa atgtgccatg
     7741 cagtgactaa ggccagtgcg gattatatca agacatcaac aggctttggt acaggtggtg
     7801 caactatgga ggatgtacaa ctttttaaaa aatatattgg acaaaatgtt agaattaaag
     7861 cttccggggg tataagaaca ttaaatgata tggaagcttt tattaacgca ggatgttcaa
     7921 gaataggaac aagttctgcc attaaattga taaataacgc ttagcgttta tttataaaat
     7981 aataaaaaaa atggaggaat taatattatg aaattatgga gaaagttggc tattgttgca
     8041 cttgtgttca taatgatttt tgctttttca gcatgtgcta acaatcagcc ggaaccaccg
     8101 gaagaaccgg aaacaccaga aacaccagaa acaccggaaa caccggaaga accggaaaag
     8161 ccggcagcta atgtttttga attagcgtta atcactgacg ttggaacaat tgatgacaaa
     8221 tcattcaacc aaggtgcctg ggaaggcgta gaagcttacg ctaaagaaca taatattaca
     8281 tacaaatatt atcaacctgc tgagcaatca gacactgctt atattgattc tattggatta
     8341 gcagtaaaag caggagcaaa ggttatcgta tgtcctggat ttttgtttga agtgccgatt
     8401 catacagttc aaacaatgta tccggaagta aactttgtaa ttcttgatgg tgcaccacac
     8461 gcaggcgatt gggttcctga cattgcagac aatacatatt caattttcta tgctgaagaa
     8521 gaagcaggtt tcttagcagg atacagcata gttaaagaag gctataccaa gttaggattt
     8581 atgggcggta tggctgttcc tgcggttgtt cgtttcggat acggatttgt tcaaggtgct
     8641 gaatatgcag ctcatgaaat gggattaaca gaccctgttc aaattaaata cacctatgta
     8701 ggaaactttg atgctacccc tgaaaatcaa gcaaaagcag cttcttggta caatgaagga
     8761 acagaagtaa tatttgcttg cggcggtgca gtaggtaact ctgtcatgaa ggcagcagaa
     8821 gcagcaggaa caaaagttat aggtgttgac gttgaccaat cagctgagtc agaaacagtt
     8881 ataacttcat caatgaagaa cctttcaaaa tccgtatatg atgctttaac agcatattat
     8941 gcaggaaact tcccgggagg tacttcagtt acattggatg ctactgttga aggtgttcaa
     9001 ttgccaatgg ataactctag gtttgaaact ttcacacagg ctgactacga tgcaatttac
     9061 gggaaaataa cagcaaaaga aatagaaata atgaatgatg cagaagttgt agaagatgca
     9121 ggaaaacctg ctgaagaagt aactgcggct gatattccgg taagcaaagt acaagtagaa
     9181 gtaattcaat agttatatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc
     9301 catagtagag gtgtgtcccc tttccatatc aggcgggcgg ggtggtaaga tggagtacgt
     9361 aattgaaatg aacaatatta ccaaaatatt cggtaatttt aaagctcttg ataatgtaac
     9421 ctttaatctc agaaaaggtg aagtccatgc gcttttggga gaaaatggag ccggcaaatc
     9481 tactttgatg agtgttttat tcggtttata tcaagcagaa aaaggcagta taaaaattaa
     9541 taacaagact gttaaaatta ataatcctaa tgatgcaagc agcttaaaaa taggcatggt
     9601 tcaccagcat ttcaaattag ttcataattt tacggttctt gaaagtatta ttttaggcag
     9661 agaaacagta aataagggta ttttaaagaa agatgatgca agaaaaaagg taatggaact
     9721 cagcgaacgt tacaagctta gaatcgaccc ggatgcatat atttcggata taacggttgg
     9781 aatgcagcaa agggttgaaa tacttaaaat gctgtattca gacagcgaaa tattaatttt
     9841 tgatgagcct acagctgttt tgacgcctca ggaaattgaa gaattaatga aaataatgaa
     9901 agaattggtt aaggaaggaa aatcaattgt ttttatatcc cataaattaa atgagataaa
     9961 agaagcagcc gaccgctgca cagtcttacg gaagggcaag tatataggaa ccgtagacgt
    10021 aaaaacaaca accaaagagc agatgtccga aatgatggtg ggacgtaaaa taagttttaa
    10081 catagaaaag gatgatattg aaacaggcga agctgttctt gaagttaaaa atttgactgt
    10141 aaaagcaaag aattcaaaga aaaatatggt taataatgtt tcttttaaag ttaaagccgg
    10201 agaaattctt tgcattgcag gtattgacgg taacgggcaa tccgagctgg tatatggaat
    10261 aacaggactc ataccgatta atgaaggaca aatattcttt aagggaaaag atattactaa
    10321 gtattcggtc agaaaaaaaa gtattaacgg aatgtcgcat atacctgaag acaggcataa
    10381 gcacggagta attctggatt ttgatttgaa acagaatgct gttttacaga cttacttcca
    10441 gccgcgtttt caaaaaagag gatttatcaa atttaatgct gtaagtgaat atgccaaaaa
    10501 attaataaaa caatacgata taagaagtcc gagagatgaa gattcaatag tgagaaatat
    10561 gtcgggagga aatcagcaaa agcttatagt tgcaagagag cttgataaag accctgatat
    10621 tgtaatagct gttcagccta caagaggact tgatatagga gctacggaat acattcataa
    10681 gcagttaatc gctcaaagag accaagggaa ggcagtgctt ttagtgtctt tggaactcga
    10741 tgaggtcatg gatgtaagcg acagaatttt ggtaatgtat gaaggagaaa ttgtaggtga
    10801 tttaaatcct aaagaaatta ctgtagaaga attaggttta tacatggccg gttcaaaaag
    10861 gagtgataga tatgagaaga actagactgt tgcaatatga aggttttacc agttttttat
    10921 cctctttgat ggctattgtg gcagggcttc ttgtcggtct tataatattg ttaataagta
    10981 attcagaaga tgcttttcct gcctttttaa cgattttaaa aggaggtttt gtagacggct
    11041 caaggggatt ggctcaagtt atatattttg ccatacccat tattatgacc ggtttatccg
    11101 taggatttgc ttttaaaacg ggattgttca acataggaac accggggcag tttacattag
    11161 gtgcctttgt tgctatttat ataggaatta aatggacctt cgttccaccg gaaatacatt
    11221 ggcttgttgc attattgggg gcaatggttg caggagcggt atggggctcc ggtcccggta
    11281 tactgaaagc atttttcaat gttaatgaag ttattacatc aataatgatg aattatatag
    11341 gaatgtacct ggttaatatg tcaatagtga aaactgtgta cgattcttta aaaaaccaaa
    11401 caaaaccggt tttaaagagc gcagtattgc caaaggccgg aatggataag ctttttaaca
    11461 ctagtaatgt cagtattgga atattcatag taattttagc tgtaatagtt atctatataa
    11521 ttctacagaa aactaccttt ggatacgaat taaaggcatg cggcagcaat aaggatgcga
    11581 gcaaatatgc gggaatcaat gaaaagagaa gtatagtgtt atcaatggtt attgcaggag
    11641 ctctatcagg attaggagga ggacttcttt atctgtcagg ctctggtaag tatatccatg
    11701 tgttggatgt attggcgccg gaagggttca gcggtatttc agtctcgctt ttaggtatgt
    11761 cccatcccat aggaatattt cttgccggat tatttatagc tcatctagaa gtaggcggtt
    11821 ttaatattca gctctataat tttgtaccgg aagtaataga tatgattatt gcagtaatta
    11881 tttactgcgg agcttttgca ttattgttta aacaaataat aagcaaattt ataattcatg
    11941 atacggatga agacattggg aaatcggtgc aggcagccga tattcctgct tcttacgaat
    12001 cctcggttgt taatgctgaa actgaagaaa aaacagagca aagggaggat aagaaatgag
    12061 tacggtttat tttttggcac agcaaactat gtattttgca attcctcttc tgatagttgc
    12121 tttgggagga atgttttcgg aaagaagcgg tattgttaat attgctttag aaggaataat
    12181 ggtagtaggg gctttttttg gaatcgggtt tattcatata tttcaagatt ctatgagcgg
    12241 tcaaagccta ttgttattgg ctgtattggt atctgccttg acaggtgcga tattttcact
    12301 gtttcatgca tttgcatcga ttaatttaaa agcaaatcaa gttatcagcg gtacagcatt
    12361 gaatatgttt gctcctgcct ttgcaattta tactgctaga atgattcagg gggttcagca
    12421 agttcaattc atggatacat tttatataag gagtgttccg attttgggag atataccgat
    12481 attaggcccc ataatgttca agaatgctta cattacaaca tatataggac tgttgattgc
    12541 tctaattgca tggtttataa taaatcatac gcgtttcggc ctgaggcttc gagcctgcgg
    12601 ggaacatccg caagctgcag attcagtcgg tattaacgta taccatatta gatatgcagg
    12661 tgtaattata tcaggtatat tagccggaat aggaggactt acatttgtag tgccgactag
    12721 tacaaatttt aatgcaactg ttgccggata tggattcttg gcattggcag ttttgatttt
    12781 cggtcagtgg agaacgaaga aaatattcgt tgctgcattt ttctttggaa taatgaagac
    12841 atttgcttct gcttattcag gtataccctt tcttaaaggt ttgccgatat caaatgaagt
    12901 ttataaaatg attccttata tagctacctt aatagtattg acattctcct ccaagaaatc
    12961 tcaggcacca agagcagaag gaataccata tgataaagga agcagataat ataaaaggag
    13021 atactatgtt tgccgataat aaaatttgga taggaaaaag tgataaaaaa gtatatttag
    13081 agcctaaaat ggctaacaga cacggtttgg tgtcaggagc aacaggcacg ggaaaaacta
    13141 ttacattaaa agtattggca gaatccttta gtgatttggg cgttcctgtt tttattgctg
    13201 atattaaagg agacttggca agcattgcaa ttccgggaac agacaatgaa aatatgcagg
    13261 agaggctcaa tcgtttagaa ataaataact ttatatacaa aggataccct gttcaatttt
    13321 gggatgtata cggtgaaggg ggaatacctg tacgtactac ggttacagaa atgggtccgt
    13381 tgatgctgtc aaggatttta aatttaaatg atacacagac aggtgttttg aatatagtat
    13441 tcagagtggc agatgagaga ggactattgt tgttggattt aaaggatctg cgtgctatgg
    13501 tacaatacgt gggagacaat tcaaaggatt tcagaatgaa atacggaaac ataacggctc
    13561 aaagtatcgg tgctattttg agaagcttga tgaccttgga agaccagggc ggtgaatttt
    13621 tcttcggaga gccggaattg gatatatttg actggataaa aacatctgca gacggaagag
    13681 gttatataaa tgttttgcac tgtgcaaaat tatttttaaa tcccttgttg tattcaacat
    13741 ttttgttgtg gatgctgtcg gaattgtatg aaaaccttcc tgaagaaggg gatttggaaa
    13801 agcctaaaat tgtatttttc tttgatgaag cacatcttct ttttgaagat gctccaaaac
    13861 aactgcttga taaaattgaa ttggtggttc ggttaatacg ctccaaaggt gtgggtgttt
    13921 attttgtgac acaaaatcca atcgatgtgc ctaatgacat attgggacaa ttaggaaaca
    13981 gaattcagca tgctttaaga gcatatacac cggctgaaag aaaaaaggtt gttacggctg
    14041 ctgaaacatt ccgtgaaaat cctgaattta acacagtgga agcaataacc gagcttggaa
    14101 caggtgaagc attaatttct tgccttgatg aaaagggaat accttctgtt gttgagcgcg
    14161 catttatact tccgccgcaa agccggtttg gaacgataga tgatatgctt agaaaccaaa
    14221 taattgccgg tactccgatt tataagaaat attataagtc agttgacaga gagtccgctt
    14281 atgaaatttt attagctaaa acggaaagaa aatatcagga agaacttctt gaaacagaaa
    14341 gacttgaaga agaaaaaaga ctcaaattag aggctaagga aagagaaaga caacaaaaag
    14401 aatttgaaag agccagaaaa agagcaagca gcaaaacaac tgccattgac agggtagcta
    14461 attcagccat gaatgcaatc ggaagagaag tgggacgttc gcttataaga ggagttttag
    14521 gcacctttaa aagataatgt ataataaaaa taagggtttt gaatacgaga aaattgccga
    14581 aaaatattta caaaataata attataaaat attagaagtt aactttataa gcaaatttgg
    14641 tgaaatagat attattgccg aaaaagataa taggctgcac ttcattgaag taaaagggcg
    14701 caaaaatatt aagcacggat atcctaaaga ggcagtaaca ttttccaaac aaaaaagatt
    14761 aagaagtgct gctaaatatt attttatgct gaaaggaaaa gacgactatc tatgtcaatt
    14821 tgacgttata gaaataatat tggaaagcag agatataaat catataaaaa atgcttttta
    14881 acaccaagtt cagattactc tgaacttgtt tttatgacaa ggggacgnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnntaa ttttactggt tgatttaatt aattatctat
    15061 gttattatca tgcaagaaat ttattgcaag gagcaagaat gaataaggat acaagtaaag
    15121 tatacaccgt ttcttttata accttgggtt gtaaagtaaa tcagtttgaa acggaagcta
    15181 tgaccgaaat attagaaaga gacggattta aagttttgcc tcccaatgaa gtaagcgata
    15241 tatatataat aaatacatgt gcggttacta aggaaagcga aaggaaatcc aggcaattta
    15301 taaacaaggc aaaaagaata aaccctgatg cattagttgt ggcagtgggt tgcagcgtac
    15361 aattaaattc cgaaaaaata aaaaaagaaa cccaagcgga cataattatt gggacaaaac
    15421 ataaaggtaa tatagggaaa cttattaaga ataagttaaa tgatataaaa caagaatgta
    15481 aaattgatga ttttcgagga agagaaagct tcgaagaatt aaaaataagc aatgtatacg
    15541 acaaaactcg tgtcaatatt aaaatacagg acggctgcag tcaattttgc tcatactgca
    15601 tcattcctta cctaagaggc cctataagaa gcagaaacca tgaggatata ataggggaag
    15661 ttaaaagaat tacaaaaaac ggttataaag aaattgtgct gaacggcatt catatatcat
    15721 cctacggcaa agagcttaaa gaaaattatt atcttattga tttgattgaa gatataaata
    15781 aaatcgaagg aattgaaaga ataaggcttg gctcaattga gcctaatttg ataaatgaag
    15841 attttatgaa aagatatgca gtattagata aagcatgcga ccattttcat ttgtctctgc
    15901 agagcggaag cgacagtgta ttaaagagaa tgaacagaaa atattcaacc aatgagtata
    15961 aaaacaatgt agaattaata agaaattata tgcctgatgc cggaattaca actgatataa
    16021 tagtcggatt cccgggagaa acagatgaag aattcaatga aaccctggag tttgtaaaaa
    16081 aaattaaatt ttctcgaatt catgttttca agtattccgt aagggaaggc actaaggctg
    16141 cagaaataaa acctcaggta aatgaacagg taaaagctga cagaagcaag gttcttattg
    16201 aattgggcaa aaatatttca aaagaattca tgaagaaatt tatcggcagg aagctgtcgg
    16261 tattgataga aaccaaaaaa aatgaaaata tttatgaagg ttatacaaca aattacttga
    16321 aagttatgtt aaaaagtgat ataaatgtta agaatgaaat aattaacatt catgttaccg
    16381 gaataaggaa cgattattta ttgggtgaat aaattagtag ggtgaggtga aaaaatggat
    16441 tgtatattct gtaaaattgc aaacaaagaa attccgtctc agtttgtatt tgaaaatgaa
    16501 tacgtggtag ctttcagaga cttagctcct caggcacctg tacatatatt agctgtaccc
    16561 aagatacaca ttacttcaat gaatgaaatt acaaaggaaa acagccatat agttgcagaa
    16621 atttttgaag caataaataa aattacagag tcagagggca ttagggattc aggctaccga
    16681 gttatcagca actgcggcag agatggatgt caatcggtac aacatttgca ttttcatatt
    16741 ttaggcggaa agaaattatc agaatcctta gcataaatta cttgcattta cttattctat
    16801 gttatataat aataaatgct atttcaaact cgctggtgtc ctaggacaac tagtagtaga
    16861 agacgagggg agggtagcaa gtaatgacag aggtaaaagt aagagaaaac gagtcaatcg
    16921 ataatgctct cagaagattt aagagacaat gcgcacttac aggtgttatg tcagaagtaa
    16981 gaaagagaga gcattatgaa aagccaagcg taaagcgtaa aaagaaggct gaagcggctc
    17041 gcagaaagaa aaataaaaaa gcaagataag aggtgaatgt atgcctttaa aagaaaaact
    17101 catggaagat ttaaaggaat ccatgaagtc taaaaacaaa gtcaaaaaag atactgttac
    17161 aatggttaga gcagctatta aacaaaaaga ggttgacgaa agaatagaat tatccgatgc
    17221 tgaaattata gatataatag ctaaacaagt taaacaaaaa aaagactcaa tacctgactt
    17281 tgaaaaaggc aatcgacaag atctagtaga tctaaccaat gaagaaatca aaatactctt
    17341 agaatattta ccacctcagc tgtcagatga agaccttgaa tcaattgtga aagaagcaat
    17401 tgaaaaaaca ggcgctcagt ccaaaaaaga tatgggcaag ctcatggcct tcataatgcc
    17461 taaagtaaaa ggcaaagctg acggaaagca tgttaatgaa atcgtggcta aatatataaa
    17521 gtagtaagaa cccattcggg ttctttttta atgacaaggg gacgcgaggg cattctcaat
    17581 taaacccttt aagggttctt tttttaattt ttcatagtaa tgtacatacc tgcataattt
    17641 atagtaacaa gaaaaatgat tacttaaaag atgacgggga gggagttatg agcaagttta
    17701 acaatttcag gagtaaatta gccgatgagc tggaaatacc gaacaatgca ttatcagata
    17761 attttgaact gagaatgcat ggcaacaaaa aagttataat agaaaaccat ttaggaataa
    17821 aggtttatga tgaaaatatg gtatgcataa aaaccaacga ccaagacata acaataaagg
    17881 gctcaaaatt taaaatagaa gaaatcaata attacaaagt aatagtaaag gggaatataa
    17941 aagaaataat atttagtatg aaggagtagt catgttaata ttcaagctga tgtatttaat
    18001 aagaggttat gtgattatcc gtttggatgc caaaaactca gaaaagcttt tgaatatact
    18061 ccaaagaaga aatattaaag tatgggatgt tgaaaagaaa ggtaaaggga taaagtttaa
    18121 aatatcatat gaagactatt taaaaaattc aactctaatt aatgatatag taaagcccga
    18181 aagcaaaaga ggatttttgc taaagcttaa caaagtcaaa ttaagaaaag gcttttcaat
    18241 aggtctgtta atattgataa taactcttta tttactgact tccatggtat gggatgttga
    18301 agttatagga accaatcagt caaatacaaa tgaaatactc gccttattag aaaaaaatca
    18361 tataaaaatt ccggtttcca tttcacgtat agaagcaaaa caattagaaa cactgataca
    18421 caaaaatttt aaatataaat ttgttgacgt ctttgtggaa ggctcaaaat taattatttt
    18481 tgtaagagaa agggagcctg acccggctga aattaaaagc aatgagccaa gcagtatcat
    18541 atccaaaaaa aatgccatta taaacaaagt aattgctaaa agcggtcaac ctgtggttaa
    18601 ggagggagac gttgtttatg aaggccagac attaattatg ggaatagtca aaaataaaaa
    18661 ttccgatgag tttatgatgg taccttccga tggcattatt tacggcaaga catattataa
    18721 ttttgaaatg aaggaagaaa aaataaaaag catgaatgtg cctacaaata aaagtaagat
    18781 tgtttattac ttgaaaataa atgaaaatag tattaaaata ttaggtaata aagaccccta
    18841 tcaaaactat gagtatagag agaatgaaat aaaagtaccg attttatcaa atatagctga
    18901 tattggcttt ataaaaggtg tatactatga agaagtgata aaagaaattg aaatagataa
    18961 aaatacagca caaaataaaa tgaaaattgc tatgtatgat gaacttttaa aaaggtgcga
    19021 cgatgacagc aggattctaa aatcaaacat aaacttttcc gaggatgaaa aattttatta
    19081 tttaatagca caaatagagg taattgaaga tattggggaa agagtaagaa ttttttctac
    19141 ggaggaagaa aagacagatg aaaccaagga ggattaatgg aattatttga gcaaaatgtc
    19201 ttaatagcaa atgaagatga attgattaaa ttattaggta ttcatgataa aaatatcaaa
    19261 attataaaaa gttattacga cgttaatatt ttagtcagaa acggagcttt aaagataacc
    19321 ggagaaaagg aaaattcaga agcagtctca aaaataataa aggacatttt aaatacgata
    19381 cgaactgaag gagatataag cgaccagaag gttatctatt taattgaagc caataaatca
    19441 aactcgcaaa ttgattacaa ccaaatttta aaagaagtaa tcgtgacatc tgcatcggga
    19501 agaattatta aaccaaagac agtcgggcag aaaagatatg tagatatgat aaattccaaa
    19561 gatgttacat ttggaatcgg tccggcggga acaggcaaga catacttggc tgttgcatgt
    19621 gcagttaatg cattcagaaa aaaagaaatt gagcgtataa tacttacacg tcctgcagtg
    19681 gaagcagggg aaaagcttgg ctttttaccc ggggacctgc aggataaagt agacccatat
    19741 ttaagacctc tttatgatgc gctttttgat ataatgggaa ttgacaactt tatgaaaaat
    19801 gttgagaagc aattgattga ggtagctcca ttggcctata tgagaggccg taccttagat
    19861 tcatcattca ttatactgga tgaagcacaa aacactacaa atgagcaaat gaagatgttt
    19921 ttaactcgtt tggggtatgg ctcaaaagcc gtaataacgg gagatataac tcaaatagac
    19981 cttcctgaag gcaaaaaatc agggcttaaa tccgtaatta atattttaaa ggatgtggag
    20041 gatataggct ttatgttttt ccacacaacc gacgtagtca gacataaact tgttcaagat
    20101 attgtaaaag catatgaaaa atacgaggta aaataatgat aaaagtattc tttgatgaca
    20161 ggcaggatgt gatggaaatc accaaagaca atgaagatgc tataaaaaat gccgtagatg
    20221 cggtcttaac ggcagaaggg ttgaatggag attttgaagt aagtgtttca tttgttacca
    20281 atgaggaaat aacggagtta aacagggaat acaggaatgt agatagtgaa acagatgttt
    20341 tatcttttcc gatgaatgaa gaatttgacg gtgttaacat tcttggagac attgttattt
    20401 caactcaaaa aattattgaa caagccaatg attttaatca cagcatggaa agagagatgt
    20461 gttatctgac cgtacacagt atgctgcacc ttttgggata tgaccatatg aataaagaag
    20521 aaaagaataa gatgagagca aaagaaaaag aaattatgaa aaaattaaaa atattcaaat
    20581 aatacagccg agggggaaga cgtgaaaaca aaaatagctg ttttaattat atgcttgctt
    20641 tttataaacg catgcgcaaa agaaactgtt acacctgaac aagcggaacc tgaaataaat
    20701 gaagaagaac cggaagaaaa accggaagtc gataaagaag aaatagagga agaaattgat
    20761 ccaaataagg tactgtcacc attggacggg ctaagatact atcctgaaga attgtcaaaa
    20821 agaccggtag ccgtatctat tgacaaccat cacagggcaa gatggcaggc aggtattagt
    20881 caagccgaaa tagtatatga agttgaagta gagcatccat ttaccagata cttatgtata
    20941 tttcttgcac atgagccgga aatgatagga cctgtacgaa gtgcaagacc atatatgata
    21001 tattatgcac ttgaaaatga cggaatattt gtgcatgtgg gaggaagcga agatgctttt
    21061 tcagaaataa aaagacttaa agttgcagat gtagacggac tttattcagg agctatgtgg
    21121 aggtacaacg ataccggaaa atatgcccct cataatgtgt acacaacatt ggaatcgatt
    21181 agaaatgaag caaaggctta cggatacaga gatgaaggaa actttgaagg atatttgttc
    21241 aatgaaaaag atgtaaaatt atcggaaaac tttgaaacca ctccgtcaaa agaagtaaac
    21301 atagtatata attattataa tactacagat tacatattta atgaggaaaa caatttatat
    21361 ttaagattta aagacgatga agaacatatt gatgaattgg ataaaaagca attaactgca
    21421 aaaaatatta ttgcagtaga aacttccaaa tcaatattgg ataatgaagg aagattattc
    21481 ttggggacca tcggcaaagg aaagggcatt tatatcacaa acggagaaag tattcctata
    21541 atatgggaaa aaacttcaga gaaagacaga ctgaaatttt attcgggtga tgaagaactt
    21601 atgttgaatc cgggcaacac atggatacag gttgttaaca gccttgaaaa tataacttta
    21661 aggtgacagg agaagtatcg aaataaagaa atgagaaagg ggacaaaatg gaggataaat
    21721 tactaataaa aattgccatg gaagcaaggg aagcgtcata tagtccatat tccaattata
    21781 aggtaggtgc tgcagtttat acaaaaagcg gcaaggtatt tacaggctgc aatatagaat
    21841 cagcatcttt tactcccacc atatgcgctg aaagagtagc tgtttcaaaa tgcatttctg
    21901 aaggttatag ggatatttac aagattgccg tggttggaag cgaaataaaa acatcttttc
    21961 cttgcggagt atgcaggcag tttttggcgg aattcggcaa ggatataaag gttatttgtg
    22021 caagaaacat agattcttat gatgtttata ccttagagga gcttttgcca aacagcttcg
    22081 gacccgatga ccttgcctaa gaaaggaaaa ttatgtttaa atcaggattt gtaactataa
    22141 taggaagacc aaatgtgggt aaatcaacat taatgaataa tatgttaggt gaaaaaatat
    22201 ctataatgtc cgataaacca caaacgacca gaaataaaat aagaacagta tatacggatg
    22261 atgaagcaca gataatattt atagatactc cgggaattca caaaccaaaa aatcaacttg
    22321 gagaatttat gaatacggaa gtggactcag cattagagga aatggacgta ttaataatga
    22381 taactgatga gtttaacaaa gtgggacccg gagatgaatt tattattgaa aaaatcaaag
    22441 acataaaagc aaaaaaaata cttataatta ataaaattga taagtttgat aaggaagctg
    22501 ccttgcgtat agcggcagaa tttgataagt ataaggtctt tgatgatata gtgcctattt
    22561 ctgcccttaa taatgttggc gtagatgcat tggttaaact gataattaaa tatttaaaac
    22621 ccggaccgat gtattttcct gcagattata taaccgacag gccggagcgt tttattgttt
    22681 ctgaaattat aagagaaaag gccctcatgt atttagagga tgaggtaccg cacggaattg
    22741 ctgttgaaat tgaagagatg aaggaaagaa aagaccgaga ccttgtagat ataagagcca
    22801 atattttatg cgagaaaaag tctcacaaaa gcattattat cggcaaggaa ggaagaaaaa
    22861 taaagggtat aggcaaaagt gcaagggaag aaatcgaaag gcttttgggg agccaggtaa
    22921 atttacagct tttcgttaaa atttccgaaa actggcgtga tgattcttat cgtcttaaaa
    22981 ctctagggta tgataaatga tacaggaaga aatcctaatt ctaaaagaaa taagttataa
    23041 agaaaatgat aaaatacttc atgccttgtc aagaacaaga ggtaaaattc ggattatttc
    23101 aaaaggctcg aaaaaaagca gctcacatct gataaatgca tcgcagattt ttgcatactc
    23161 aacgtgcaca ctcaatgtat caaaagatat gtacatatta acttccgcca aacttttgga
    23221 taatttctac aacttaaaca agaacatggc tgcatattat aacgcatctt atatattaga
    23281 gcttataagt tatgtagcac aggaaaatga agttgattcc agagtatttg atatgacggt
    23341 atctgtgatt tctcatttaa gcaattgcac atatgaacat gaaaagctta ctgctgccta
    23401 tgaattaaaa ttggcggcaa tgcttgggta caagcctgat tttctccatt gccttcgttg
    23461 cggctcttca ataaacgatg aaagtaagtt ctccgtaaca gaaggagggg ttttttgctc
    23521 taaatgcgta aattttggaa atggaataaa tgtaacatat aatgaaatac taattatgga
    23581 taaaatttta aaatctaaat ttgagaatat tggctttata gaaataccta agactataat
    23641 gaacttaata agaaaatttt tgttttatta tatagggaag gataatttta ctacattaaa
    23701 gctattgtaa aagaaaggat gttcatatgg aaaatgatac tttaaacaaa atcgacgaaa
    23761 ttataaagcg cacaaatact gattacggta cagcaaaaga agctttggaa gctgcaaacg
    23821 gtgatgtgct tgaagctata atactgattg aaaatcagca aaaaggcagc acgggctcct
    23881 ttagaggaga gcagataatg gctcaactga aggatatatt agctaaagga aatgctacaa
    23941 aactcacaat taagaaggat aatgaagtta tagtcaatat accgataact gcaggcttgg
    24001 taagtgcttt tattgcgcct tttctttctg cagcaggaat aaccgtagca ctgcttgcaa
    24061 aatgctctgt ggaaataaca caaacagacg gtaaaataat agatctcggg caaaaagttg
    24121 atgaaggaat cgatgctgta aaagatgcta tgcatgatat aaaaaaaggt gcacagaatt
    24181 taaaagatga tttaagaaca ggctcaaaaa atattaagga tgatataaaa acaggtgccg
    24241 aaaatgtgaa ggataacata aaggccgaag ctgaaaatat gaaagatgat ttaaaaaatt
    24301 cttttgacag aaataatgtg catttttaaa aagctcttga cataaaaaga ttattttgtt
    24361 acattatagt aaattaagac tatgaaaaag aagagtattc tatgcttaat ttacagagag
    24421 tcagataagg tgaaaactga taaggatgta taggagaagg gagcttttga gtctatactt
    24481 ttgagaggat gaagctttag tgctttgtca aatagggtgg aaccgcggaa gtaaatcttt
    24541 cgtccctata tgggatgaga gattttttat gttctcttcc atttataagg aatggtacgc
    24601 ctgcgtactt ttaatatatt tataacttaa aattattata catatggagg aatttatggc
    24661 aaagattgaa aaaaacagct tagaaaaagt agtatcatta tgtaaatcaa gaggaattat
    24721 ttttccgggc tctgaaattt atggcggctt agctaacaca tgggattacg gacctttggg
    24781 agtagagatt aagaacaaca taaaaagagc ttggtggaaa aaatttatac aagaaagccc
    24841 atataatgta ggattagatg cagcaatact tatgaatcct acaacttggg ttgcatccgg
    24901 tcacgtggga ggattttcag acccattgat ggactgtaaa gagtgtaagg aaagattcag
    24961 ggctgataag cttattgaag attttatgaa agaaatagat gacgtacaag tggttgatgg
    25021 ttggacaaac gatgaaatgg aagcatatgt tgcagaaaaa aaagtaaaat gtcctaattg
    25081 cggggcacat aattttacaa ccattagaaa attcaattta atgtttaaaa cttttcaagg
    25141 tgtaaccgaa gattctcaat ccgagcttta tttaagaccc gaaacagctc agggtatttt
    25201 tgttaacttt aaaaatgttc agcgctccat gcgaaagaaa attcctttcg gtataggaca
    25261 aataggaaaa tcattcagaa atgaaattac accgggtaac tttacattca ggactagaga
    25321 attcgagcaa atggagttgg aatttttctg caaacctggt gaagacatgg aatggttcta
    25381 ctactggaaa gaattctgta tgaactggct tctaaacctt ggaatgaata aggaaaaact
    25441 acgtttcaga gaccatgaac cggctgaatt gtcacattac agtaaaggta caacagacat
    25501 tgaatatttg ttccactttg gctggggaga attatggggc attgcaaaca ggactgattt
    25561 tgacctgaaa cagcatataa acacatccgg agaggattta tattatcagg atcctataac
    25621 caatgaaaaa tacataccat actgtgtaga gccatcctta ggagcagacc gtgtaatgct
    25681 ggcaattctg gttgatgcat ataatgaaga aacgctagaa gatggaaccg atagggttgt
    25741 gttaaagctt caccccgcat tgtcaccata taaagcggca atattcccgc ttacaaaaaa
    25801 attaaacgag caggctatag agctttatca aatgcttaac aagcatttta atgtagatta
    25861 tgatgacgcg ggaagtatag ggaaaagata cagaagacat gatgaaatag gaactcctta
    25921 ttgcattaca ttcgattttg attcattaga agacaataca gttaccgtaa gggatagaga
    25981 cacaatggag caggttagaa ttaaaattga tgatttagta acatttattg atgaaaaaac
    26041 atccttttaa agatgctttc cccatttttt tgagtgtttt ttctgtttac aatccagaat
    26101 tctttggagg tgtattttga atatcagaga aaaatacgag caattcgaaa tagagcatct
    26161 gtcagagtat gcctccaagg cagcactctc aaaaggaaga atgctttatg agccgaaatg
    26221 tgaaataaga acggattttc agagagacag agacaggatt ttgcattcaa aagctttcag
    26281 aagacttaaa cataaaacac aagttttttt atctcctgaa ggtgaccact acagaacaag
    26341 gcttacgcac acgcttgaag tatcgcagat atcaagaaca atagcgagaa gcttgaattt
    26401 aaacgaggat ttaacggaag ctattgccct tggacatgat ttaggccata cacctttcgg
    26461 tcacacggga gaaagaatac ttaaagagct atgcaacgga tttaaacaca atgagcaaag
    26521 tctaagagtc gtggattttt tggaagtaag aaatgaaagg caagggctta atttgacttt
    26581 tgaagttaga gacggtatat tgaatcatcc ttcggggtat aaacctgcta ccctggaggg
    26641 gcaaatagta agcatttcag acagaattgc atatataaat catgatattg atgatgcggt
    26701 caggggacat attattacta aaattccgga caaatttcta aagaatttag gctcaagtca
    26761 cggtcaaaga attaacacaa tgataatgga cataattcaa aactcggaaa acaaaaataa
    26821 aattgtaatg agcgatgaag ttgccgagct gacggatgag cttcgtaatt ttctgtttga
    26881 aaatgtatat tataataaag ttgccaaaag cgaagaagat aaaacaatgt tcatcgttga
    26941 aaaaatattt gaatattaca ctaataattt tgaacaactt cctgaattct atattaatat
    27001 ttataataat aataacttca gcaagggaga aattataaaa gattatatag cgggtatgac
    27061 agacagatat gcaatgaagg tttttgaaga actgtatata cctaaaccct ggatataaaa
    27121 aataaaaaaa gaagcagaaa atactatatt acaagaatca gaaaatttta gggcttgtca
    27181 aaaaaaatat tgacaagctt attttcctat gttatacttt attttcaatg taaggataat
    27241 ggtgatatta tgccctatag gttggattct gacgttatac aaagaatctt ggaacaaaat
    27301 aatattgtag atataataga agaatattta cctttaaaaa aagcaggtgc aaattacagc
    27361 acaacctgtc cttttcataa agaaaaaaca ccttcattta tagtttctcc ggataagcaa
    27421 attttccatt gctttggctg cggtgaaagc ggagatagta taagttttct gacaaaatat
    27481 aaaaattata catttgttga agcagctgaa tatttagcaa acagagcagg tattatcttg
    27541 gaagaaatta actcatcaag aattaacaat aataaacaaa cagcagatat gctatacaaa
    27601 attaacagag atgctgctgc ttatttttac aagaatttaa ggaagagtcc taatgcaatt
    27661 aaatatttat tttccagaaa tattaaatca gatgtaataa aatcgtttgg aattggatat
    27721 gctattgacg aatgggataa tttgttaaat tatctaaaaa atgaaggtta taatgaagaa
    27781 gatattttaa agaccggatt aattatttgg aacgataaaa aaaatacata ttttgataga
    27841 tttagaaaca gaataatttt ccctatattt gatattaaaa aaagaattat aggttttggg
    27901 ggaagagtaa tagatgactc tatgccgaaa tatcttaatt ctcctgaaag ccttattttt
    27961 aataagggat ataatttata cggaattcat atagcaaaag aaaatataag aaataacagc
    28021 tttattttgg ttgaaggata tatggatgta atcaagatgc atgcccatgg ttataaaaca
    28081 gcagttgcag ccttaggcac atcttttacc gaaaaccaag caaaactgtt aaaaagatac
    28141 tctagagatt tctatatagc atttgattcg gatgaagcag ggcagaaagc atctttaaaa
    28201 gcaattaata tgctaaaaag aaataactta aatgcaaaaa tagttataat gaaggatgcc
    28261 aaggacccgg acgaatatct gagtaaatat ggaaagcccg ctttcgacaa attaattgaa
    28321 aattcattag attattatag ctttttagaa tttcatttta aagaaatact tgaaaattct
    28381 aacaaagttg aatatataaa caatcttttt gacaatatag taaatgtaaa cagcgaaata
    28441 gaaagagaat tgatttttga taaattatca agaaaagtag gtgtttcaaa agagtccata
    28501 attaaagaat tcaataagag aagtactaaa caaaagactt tggataagac tgcaataccg
    28561 gcggtaaaac cgcaggttgc aaaaattact acatgccacg aagaagagct gattaaactg
    28621 atactattag acaatgattt tgcactcaag ctaagtgaga tagtgaatga agataccttt
    28681 aaggacttaa aatactataa tatattaaaa gagctttatg aatgcagaat taacgataat
    28741 agtattgaca gtaagagctt aaataaaatt ataaaggacg acatgaatgt aaatttgcag
    28801 tttgacaatg ttgattatga caatttagag gctttgttcc aagattgtat gaaaagactt
    28861 aaaataaaat attataagga aaagagaagc atattgaata acattttaaa acaatctgat
    28921 aattcattaa accgtaagga aattatgaat gaaatttatt gcctcgctaa aaagattaaa
    28981 tcaactaagg aggaggtcaa ctgacaatga gtgaaaataa aaatatatca gtaatttcag
    29041 aagaagaata tgcaaaaaac gaagaaatgt tacaaaaaat agactccata aaaaataaat
    29101 taattgaaaa aggcaaaaga aacggtttta taacatataa agaagtattg aggtcatttg
    29161 aaaagctaga tattaatcct gaattcatag atgattttta taagcaagca gaagacaacg
    29221 accttgaaat attaggtttc caagacgata tgatacctga aaaggtcgat gaagaagatg
    29281 aaatagattc aaaagaagac gcggattttt ttgtaagcca atcagatgat gatatcctta
    29341 aaggtgtaaa tatagatgac cctgtaagaa tgtatctgaa agaaatcggg aaggtttctt
    29401 tattgagtgc acaagaagaa gtagagctgg ccaaaagaat ggaagccggt gatgaagaag
    29461 ctaagaagca gttggctgaa gccaatttaa gattagtagt aagcattgca aaaagatatg
    29521 taggaagagg aatgcttttt cttgatttaa ttcaagaagg aaatcttggg cttataaaag
    29581 cagtggaaaa attcgattat acaaaaggtt ttaaattcag tacatatgca acatggtgga
    29641 tacgtcaggc tattacgagg gcaatagcag accaggcaag aacaataaga atacctgtgc
    29701 acatggttga aacaatcaat aagttaatca gaattaaaag acagctattg caggaattgg
    29761 gaagagaccc tacaccggaa gaaatagcat tggaaatgga aatggagcct gaaaaagtta
    29821 gagaaatact taaaattgcc caggagcccg tatctttgga aacacccatc ggtgaagaag
    29881 aagacagtca ccttggagat ttcattcccg atgatgaagt tcaggcacct tcagatgttg
    29941 caacattcac ccttttaaga gagcaactgt caaatgtgtt gcatacttta acagacagag
    30001 agcaaaaggt actgaggctt cgtttcggtt tggatgacgg aagagcaaga acacttgaag
    30061 aggtcggtaa agagtttgat gtaacaagag aaagaatacg tcaaatagaa gctaaggcac
    30121 tgagaaagct gagacatcca agcagaagca aaaaattgaa agattattta gaataaagat
    30181 gccctaaccc tatttgttca atgtatgggg acacacctat actataggga tagtctttgt
    30241 agttagagga atgtccccaa attaggtttt ctgaaaaata gtggtaggtc attcgcaacg
    30301 agcaccaatt ttgtgcgacg aaagtagcaa acaaaattgt gtagggagac tgcgtgtaac
    30361 actctgaatg ataactatat taatatatag atggaatgca acatttacat tccatttatt
    30421 catttaaggg ggtcagtatg aacagattag aatgtattgt atcaatggtc gataaatgca
    30481 acacagcagc agatataggt actgaccatg gatatgtggc agagatgctt ttaaaaaaag
    30541 gaatatgcaa taaaattata gcgactgatt taaacaaagg acctttatac agagcaaggg
    30601 agcatttatc cgaagtaaaa ctcagtgata aatgtgactt taggcttgga gacgggctta
    30661 ctgttttaaa tgaaggggaa gcagatacaa ttattgttgc aggaatgggt ggcgatttaa
    30721 tttcagaaat aatcgaaaga tcaaaacata tagcagagag tgcaaaacag cttattttac
    30781 agcctatgac taaggttgat aatttaagaa gatacctttg taataacggt tttgaaataa
    30841 tcgatgaaaa tatagtaaag gaatacaatc atttttattt tgtaataaag acaaaaaaag
    30901 gctttaccaa atatatggac gatatatatt atgaaataag taaagtatta acagataaaa
    30961 gagataccgt aatgatagaa tacataaata aggtaattga tacaaacaaa aaaataatca
    31021 gcagtcttga aaaagcagat aatgtggcat ataatgaaaa aatagaggaa ttaaaaagaa
    31081 aaaacaagaa aattagggaa ttaataagat gaatttaaat aatattatta atttattaaa
    31141 tgaagaagta aagttgaagg agcatgaatc ctgggacaac agcggattac agctgggctc
    31201 catggaagtt aacataaata aaattatgtt aaccttggac atcaataagg aagcaattga
    31261 atacgctgtt caacataagg ttgatttaat aattactcac catccgtttt ttttcggcag
    31321 tatcagaaaa attgattttg atacatacga cggtgaaatt attaaaatgt taattaaaga
    31381 aaatataaat gtttacagca tgcacacaag ttatgacatg gctgataagg gagttacata
    31441 tagcttggca aaaagactga atatattaaa ttatcaggtt cttcatccaa taaatattga
    31501 taacagcgga tatgggggaa taggagatgt agctcccgtt aatataattg agtataccaa
    31561 attagtaaaa gaagctcttg gagctgaata tgtcaaattg tattgcaatg gagaaaaaac
    31621 agttagaagg gttgcatttt gcggcggcag cggcggtgaa ttcattgagg atgctataag
    31681 gcaaaaagct gatctttaca taaccggaga tataaaatat caccaggcac aggaagctgt
    31741 aaaaaataat ttatgcatta ttgatgcagg tcattacaac acggaaatat actcaatgga
    31801 aacactaatg gaagtattag aaaaaacagg attggaaata attaatttta agaaaaatat
    31861 aacagctgaa aaaataattt agaggtggat atggataaga attttgaata tataagagtc
    31921 gcttcggcag taccatccat aagagttgca gacccaaatt ataatgcaat taaaattaag
    31981 gaaataatat taaaagcgga aaaagaaaaa gtcagagtct tggtatttcc tgaactttgt
    32041 ttaacgggat acacttgtca agatttgttt agccagaata cattgataaa taaatgcgaa
    32101 gaagcattaa gccttttatt ggaagaaaca aagtatttga atatgcttat agctgtggga
    32161 atgcccgtaa aagccgacac caagctgttt aattgcgccg taatatttaa ggaaggaaaa
    32221 atacttggtg ttgttcctaa aacatatata cctaattaca aagagtttta tgaaatgaga
    32281 tggtttgttc ctgcatctta caggttatca gacatagtta cactttgcga gcaggaagta
    32341 atttttagcg aaaacctttt gtttaaggat gatatatctt cgctatgtgt tggagtggat
    32401 atatgtgaag atttatgggc aactattcct ccaagctcat atcatgcttt gcagggtgcc
    32461 aacttaatac ttaatttgtc cgcgagcagc gaaacatcag gtaaggcaga ttaccgaaga
    32521 gatttggtaa agatgcaatc ttccaaatgc ataacatcat atgtttatac ttcttccggc
    32581 gaagaagaaa gcacaactga tgtagtattt tccggacact gcataattgc ggaagacggc
    32641 aaaatattaa aggaagataa tttatgcgac ggtttcatat attcagatat tgatatagaa
    32701 aaaataaata atgaaaaaag aagcttaaat gtttataatt caaatataga aagaaaaaat
    32761 tacaaatatg tcaattttaa tttaggctac agtgaaaatt tgaagcttga aaggtttgtt
    32821 gagcccagac cttttatacc tgcaagaccc gaagacagag ataaaagttt ttctaatatc
    32881 ctaaaaattc aggctgcagg tctttctcag cgcatgaaaa agattggttc aaaaaaagcc
    32941 attctgggga tatcaggagg tctagacagc acattagcat tgattgtggc ggttgaagca
    33001 attaagaaat taaaattacc gcttacaaat ataattactg ttattatgcc gggatttgga
    33061 acatcagaga gaacttacaa aaatgcccat atactgattg atgaattagg agtaacctta
    33121 cgagaaatat ccataaagga tgcatgcatg caacattata aagatatagg acatgatata
    33181 aacaatcatg atgttactta tgaaaattca caagcaaggg aaagaacaca aattttaatg
    33241 gatattgcca atcaagaagg aggactcgtg gtaggaacag gtgatttatc cgagcttgcc
    33301 ttaggatggt gcacatacaa cggggaccat atgtcaatgt acagcgttaa ttcaggagtt
    33361 cccaagacct tgataaaata tatggttaag tggtatgcgg attctaaaac atcaggagaa
    33421 atgcaaaaaa cacttcttga tatatgcaac actcccgtaa gccctgaact gcttccaccg
    33481 gacaaggaag gaaatattcg tcagcttacg gaaaactcgg taggctcata tgagctgaac
    33541 gactttttcc tgtacaatat gatgaaaaac ggttacggac cggaaaaaat gtactatttg
    33601 gcaaaaatag cttttaaaga agaatacgac gaacaagtta tccttgaaac attaaaaaat
    33661 ttctataaaa gattttttac tcagcaattt aagcgctcct gcatgcctga tggggttaag
    33721 gtaggctccg tatcattgtc gcccagagga gattggaaaa tgccaagcga cgcatcttat
    33781 gaattatgga tgaaaatatg cgaaaatatc aaaaaataaa attcttggat aaatctgcat
    33841 ttgacataaa attataaata ttataaaata acaaagagta aattatacaa tcgcatgcat
    33901 agcatgagga aagtccgagc tccatagagc agggtgccgg gtaacccccg gtgagggtga
    33961 ccttaaggat agtgcaacag aaagaaaccg ccccgcactc aatctgtaag agattcagtt
    34021 tattatttaa gatatgtaaa ctataaatta atttagtata gagtctttta cagattgagt
    34081 gcggggtaag gatggaaagg tgaggtaaga gctcaccggc aatcaggcga ctgattggcc
    34141 atgtaaaccc cacccggagc aagaccaaaa gaggaccttg gagtggctgc tcgtcacggt
    34201 ccaaatggta ggtcgcttga gtttgccggc aacggcaagc ctagatagat gattgtttaa
    34261 tacagaactc ggcttatata tttactcact acatatataa tagaaaataa aatcattagc
    34321 ctttgctaag acgaaggttt ttttgttata atttgtaaca atatcatctt cattgaataa
    34381 aataagtaca ggcaatataa aaagggatga tgatatgttt tgtagagtag tgccatggtt
    34441 tttacttggt gttggtttcg gaagatgcag cgagagacgt gatgtcttag gagctagggg
    34501 aagatttgac ccatgcatca gaagaagaag ataataaaat aagggacata acttcggtat
    34561 tatctgcagt gggcagccgc aaggtgtgtc ccattctctt atatatagat tcaggctgta
    34621 tatttagatt ttattgcggg ctttttcggc tgcaataaat ttttttgcaa ttaatgcaat
    34681 tgttaataaa attatggaag ctgcaattac ccaaattgcg ggtattgata taaatcttat
    34741 taaaatactg tacattaatg agccggataa gcctccgatt tgtattgtaa atgattttac
    34801 cgacagcacg gaagccctta tttggttggg agtttcagaa ttaagtatta catcttccgg
    34861 aatattagcc atgccgaaca ttaaataaat tgaggcataa taggcaataa acaagggaat
    34921 atttgtttgt aatgcggtta gtatcaaaaa tagagcaagt aagcttctca ttattagata
    34981 catctttctt atgttaaatt tatatttgct cataattttg tttgaaagaa tacttcctac
    35041 caaagctgcg cccaaatata aaaatgccat caatcctaag agacccgagg agccttcatc
    35101 gggtaaaagt gttataaagt gaggctgcca ataggtttct aatgcgctta gaaagatacc
    35161 tgttgaaaaa acagaaatga atataaaaag tatggtggtg tttttgcgaa caaaatcaga
    35221 actgtttttt acatgttgtt taatggattt atgttctttt ttatcatgat ggtttttttc
    35281 cgaaataaaa agaacagata taattatgac agcaattgtt aaaacaattc taacaataat
    35341 atttaaatca taagttccta tggaaggaag aagattatta gaaatatccg ggaagaatcc
    35401 tccggataaa gcacccgctg aaagtcccaa tgcatctaag actgaaagcc ttgttgtaat
    35461 gttatgtaat ttgtccttac cgaaattatc tatatagtag tcaatgaata gcgcatcaaa
    35521 agatcctgag ccgagagctc gtcctaaacc gtaaagaatc atcacaatac ataatactat
    35581 aaagactttg ctaatcaata ataatgaaaa acttaaagta aataatacaa gggataatac
    35641 aaaggttttt tttctaccga agacatctgc cattattccg gaaggcagtt caagcaaaat
    35701 aaccgacaag gcatataatc ctaatataat tgataagtta ctcaaggagg cgcctttatc
    35761 aattaaagcc agactaagta ccggggttaa cagaccggtc aaatatgaat taagaaacat
    35821 tattaaagaa tagatgtgca gcttcaaatt tctacctcca aaggtaacta ttgcaccgta
    35881 taataattat aatctaaaag agtattagag tcaagcgcaa ttaaagcata tattgcgcgc
    35941 tctgcggtca aaaaataatc agcttttatt agagaaatac aaaaaaagac gca
//
LOCUS       DAOSHP010000010        35936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_930_length_35936_cov_9.014102, whole genome shotgun sequence.
ACCESSION   DAOSHP010000010 DAOSHP010000000
VERSION     DAOSHP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 35936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35936
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_930_length_35936_cov_9.014102"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLB33_02015"
     CDS             <1..270
                     /locus_tag="PLB33_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOG62327.1"
                     /translation="GEDTASSVIDGTEIYLPLADLIDYEKEIERLEKEKTRLEGELKR
                     VNDKLNNERFMSKAPENVVNEEKEKLQKYQLMMEKVLERLEQMKK"
     gene            495..1511
                     /locus_tag="PLB33_02020"
     CDS             495..1511
                     /locus_tag="PLB33_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOG62328.1"
                     /translation="MDYNELLNKAREKMAPRCRVCKECNGVVCKGEVPGVGGKGSGNA
                     FKVCIDFLASVKINMDTIYKNEGQDTSISLFGKNFKYPFFAAPIGGMVLNYNGSMTEA
                     EYAEAIVNGTLKAGCAGFTGDGPMDNLFLDSLPIIKKANGIAVPTIKPWKNDKALEKI
                     KMIEEVNAMAFAMDIDSAGLINLALAGKPVSAKSVEELKELTDATNLPFIIKGVMTAK
                     GAEKAYQAGAYGIVVSSHGGRVLADAPATCAMLPEIREAVGDKLKIFVDGGIRSGADV
                     FKAIALGADAVLIGRPYAIAAFGGGIEGVEMYTEKIGAELRETMIMTGCKNLSDITRD
                     KIIL"
     gene            complement(1584..2384)
                     /locus_tag="PLB33_02025"
     CDS             complement(1584..2384)
                     /locus_tag="PLB33_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG62329.1"
                     /translation="MKRRIIALTLALVIALSSTSAFAATTNCTSVNYKYNLKDFIKTN
                     TSNYANKNTQYKITVNGKTVDLKSIDWKTVLKQYAPAQKPAAPVEKPSPAPAPVEKPA
                     PKPAPVEKPAPAPAPVEKPAPAPAPAPVENNTGVPSSNLTYEQKVVELVNIERQKAGL
                     PALKMDSAISNVARTKSKDMAVNNYFAHQSPTYGSAGDMLRQFGISWRAWGENIAAGQ
                     RTPEIVVDAWMNSPGHRANILSSNFSKIGVGYATNSSGRPYWTQIFTN"
     misc_binding    2729..2925
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2962..4194
                     /gene="tyrS"
                     /locus_tag="PLB33_02030"
     CDS             2962..4194
                     /gene="tyrS"
                     /locus_tag="PLB33_02030"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOG62330.1"
                     /translation="MEKGNVFDVLVERGYVEQCTHEEEIRELLGKESVTFYIGFDPTA
                     DSLHIGHFIQIMVMSIMQKYGHRPIALLGDGTTMIGDPSDRTGMRSVMSFETITKNAE
                     NFKKVFEKFLDFTDGKALMPRNSEWLLPLNYLEFMREVGVHFTISKMIAADCYKNRMD
                     QGLTFFEFGYMLMQAYDFYILNEKYDCKMQLGGNDQWSNIIAGVELTRKKAGKQVYGM
                     TFALLTNSEGKKMGKTEKGALWLDRNKTTPYEFYQYWRNIDDADVEKCLALLTFLPMD
                     EVRRLGALKGAEINKAKEILAFEITKMIHTEEDAVNAQEAARALFGGGADSENIPTTE
                     LTKAELGESISVIDLMLKAGLIKSKSEGRRLIEQAGVAVNDAIIDDIGATVTEADFED
                     SKLMIKKGKKTYHRIKLS"
     gene            4285..4458
                     /locus_tag="PLB33_02035"
     CDS             4285..4458
                     /locus_tag="PLB33_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62331.1"
                     /translation="MINLFEIDLNMIASFAIVFGIIIYVGYVNTSIRELKKRVEELEN
                     KIGIDKKADEELK"
     gene            4613..6001
                     /locus_tag="PLB33_02040"
     CDS             4613..6001
                     /locus_tag="PLB33_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG62332.1"
                     /translation="MLSRKLIENTLTAALSTGGDFAEIFVEDRTNNGIVMIGGKVEST
                     MSGRDYGVGIRIFKGFNSVYAYTNKSDEDTLIETAKKAAQAVEGNIIDLTINLTKSDV
                     KNINIIQIDPSKVEKTKKVQVMKKAYDIAFNYDELITQVSINYTDYIQNIMVANSEGL
                     WKEDTRVRSRLGISSVASKDGEMQSGFFAPGASKGYEFFENLDIDYYAKEASRIAVTM
                     VKAKYSPSGRMPVIIDNGFGGVIFHEACGHGLEATSVAKGLSVFAGKIGQQIASPIVT
                     AIDDGSIPNEWGSFSIDDEGTQSQRNVLIENGILKGYMIDKLNARRMNMDITGSSRRQ
                     SYRFPPTSRMSNTFIDNGSSTPEEIIANTEKGLYAKHMGGGSVNPATGEFNFAVMEGY
                     IIENGKIKEPVRGATLIGKGMEVLNKIDMVGNNLLQSQGMCGSVSGSIPANVGQPMIR
                     VSELTVGGREGE"
     gene            6004..>7271
                     /locus_tag="PLB33_02045"
     CDS             6004..>7271
                     /locus_tag="PLB33_02045"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOG62333.1"
                     /translation="MEMKSLIDKIFQKGREKGLNDMEVYYSAGSSLTLKVFQKELETY
                     NLSESEGLSLRGVYKGKMGYSYTEKVDETSIDQLVRDVLENAVIIDSDDEEFIFEGSK
                     EYIKVDSFNPALSNVSEEKKIEFVKSLEEEAFKLDKRIESVEVCVYGDGYGEIIMSNT
                     KGLFLQDKSNIAYTYIVVVAKEGEDIKTGMAYRTGNDFNKFNARDIAKEAVDEALALL
                     GAKSVKSGDYNVIIRNSAAADLLEAFTGIFSAEAVQKNLSLLKGKLNEKIASDKFTLI
                     DDPYMESGLASKSFDGEGVACKYKKVVDKGVLKTYFHNLKTAKKDGVETTGNASKGSY
                     KSSLGISPSNFYVEKGERRLNEMITDINKGILITELQGLHSGLNTVSGDFSLAALGFL
                     IENGKISRPVEQITVAGNYFEMLKNIEETGS"
     assembly_gap    7272..7371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7373..7570)
                     /locus_tag="PLB33_02050"
     CDS             complement(7373..7570)
                     /locus_tag="PLB33_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOG62334.1"
                     /translation="MDKFALFLVIIGAINWGLIALFQFDLVAAIFGGQTALFSRLVYG
                     LVGLAGLYCITLLFNERERVD"
     gene            7730..7996
                     /locus_tag="PLB33_02055"
     CDS             7730..7996
                     /locus_tag="PLB33_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62335.1"
                     /translation="MECNKPENIVEAKADVNTQGDCVRGDRGRRDCIRRDRDRVMGTG
                     GIDSSLLFFFLLLVIIVDQSDCDIEFDTLLWFFLVLVAIFCKCD"
     gene            complement(8482..9159)
                     /locus_tag="PLB33_02060"
     CDS             complement(8482..9159)
                     /locus_tag="PLB33_02060"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62336.1"
                     /translation="MIKLINISKKYDNLILLKDFNIDFEKNKITCLFGPSGVGKTTIA
                     NIVAGIVPVDEGRVVGIDNSLFSYVFQEPRLLKWYNVYENIDFVLKDVYKPDKRAEII
                     NSYLEMVELSEYRNYKIDALSGGMAQRVSLARAFAYPSHILILDEPFKGLDNKLKGEM
                     SSLFLKMWNLSQRTVIFITHDVEEAVDLSHIIYTLKDRPVNIIDRLIVKNYANNKEEI
                     KNLLLNH"
     gene            complement(9152..9901)
                     /locus_tag="PLB33_02065"
     CDS             complement(9152..9901)
                     /locus_tag="PLB33_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOG62337.1"
                     /translation="MMNSILRNKNKLLIILFWIFVWEILSLIIAQEIYLPSPHAAFSS
                     LMNLLISKDTYITIYYSTYRTLTGFFLSCVFGIFLGIISGIHKTVYNLLNPLIVIIRT
                     IPVMSIIIIAIMWFKDTNVPIFVAFLMCFPIIWTNTVSGILSTDVKLLQMCEVYKIKK
                     SRIISSVYFYSSLPYIKAGMISALGIGWKVTSAAEVLSLPKYSIGRFLYDSKVYLEIP
                     DLFAWTIIIVSLSFLFESLLKTIFKFGDKYD"
     gene            complement(9876..10901)
                     /locus_tag="PLB33_02070"
     CDS             complement(9876..10901)
                     /locus_tag="PLB33_02070"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG62338.1"
                     /translation="MKKISFLLVVLIVLIFTAGCNNEPAETPTPDENAEETPMPEEPA
                     SEPVNVTIAGLKGPTSIGMIKMIDEKALKSDKYNVEYIQESAPDSLTGKIINGDIQIS
                     SVPINLASVLYNKTQGKVQLLAVNTIGNLYIVGTEDISSVSQLECKTLGMSAKGSTPD
                     FAMNYILKQAGLEGKTELNYALDHATLAQSVIAEDTRVALLPQPFVTQTTLKNPNVKM
                     LIDLNEAWNDATGGKSELFTGCIIINKEFAENNPEFVDEFLKQYENSVNWVLENQKEA
                     SVLTAKHEIMPDAVLVEKALPYCGITFRKAAEAKESLNNFYQILFDSNPASVGGSMPD
                     DEFYFKE"
     gene            complement(10903..11157)
                     /locus_tag="PLB33_02075"
     CDS             complement(10903..11157)
                     /locus_tag="PLB33_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOG62339.1"
                     /translation="MDKRIGVIGIVIEDTSQIAEVNRILHEYSHIIIGRMGIPYREKG
                     LNVISIIVDGTNDDIGALSGKLGKIKGLSVKSALSKNIRR"
     gene            complement(11306..11503)
                     /locus_tag="PLB33_02080"
     CDS             complement(11306..11503)
                     /locus_tag="PLB33_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG62340.1"
                     /translation="MLGERLRQIRKENNLTKQELANALNVPKERIKLYETGEEIPSDF
                     YLSSFVQYFNISKEELIELIK"
     gene            11642..12079
                     /locus_tag="PLB33_02085"
     CDS             11642..12079
                     /locus_tag="PLB33_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62341.1"
                     /translation="MISTRWFQGKSNLSDVLSIRKEVFAEENREENITDFYDEFAFNA
                     VLYEDDVPAVTARLSFKEGKYFIDKLCIKKQFTGLRYEDLLVRLLVRKAVTIGAEKTY
                     IEADESIEKILENIGFTKVPNNKTLMVKEGDVGGHCCGDCPEK"
     gene            12268..12339
                     /locus_tag="PLB33_02090"
     tRNA            12268..12339
                     /locus_tag="PLB33_02090"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12301..12303,aa:Glu,seq:ctc)
     gene            12430..14076
                     /gene="gltX"
                     /locus_tag="PLB33_02095"
     CDS             12430..14076
                     /gene="gltX"
                     /locus_tag="PLB33_02095"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOG62342.1"
                     /translation="MDYKKLAELLFPNITKTIEYYENEVYPKRKLPEGAKVTRLAPSP
                     TGFIHLGNLYGAFVDERLAHQSGGVFLLRIEDTDEKRKVEGSVETIITSLEYFDLKFD
                     EGAGIEGETGDYGPYFQSNRAEIYQCCAKHLVEMGRAYPCFCTEEELEELRKEQAKEN
                     VNTGYYGKWARDRNLSLEEVQQHLNNNEEFVIRFRSEGTEEGKFEIVDAIRGRLIMPE
                     NYQDIVILKTNGIPTYHFAHVVDDHLMGITHVVRGEEWLSTLPIHYEIFKTIGWETPV
                     YCHTAHLMKMDNGAKRKLSKRKDPELGLGYYRELGYHPKAVREYLLTILNSNFEEWRM
                     KNPENPIDDFQFTLGKMSNSGALFDLDKLNNICKDVLLKIPAAEIYEFLLNWANEYKK
                     EAYELLKSNKDEVIKLLSVGRDGNNPRKDLMYCEQILDFISYYFDEYFKIVDSYPENI
                     DSEEAKKLLKAYLSTYDHGDDQSQWFEKIRVIASENGYAAKPKDYKKNPDMYKGHVGD
                     VSTAIRIAIIGRASSPDLWELQQIMGEEKVRRRIENAINS"
     gene            complement(14202..16121)
                     /locus_tag="PLB33_02100"
     CDS             complement(14202..16121)
                     /locus_tag="PLB33_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOG62343.1"
                     /translation="MGLTAQSVKLESLRDIYEIEKNDKQYVVALAGNPNTGKSTVFNY
                     LTGLNQHTGNWPGKTVVSARGQFKYKNNEYILIDLPGSYSLFTASKDEEVTRDFLCYG
                     NYDTVVVVADATCLERNLNLVFQVSELTNKIILCINLIDEAKKKNIKIDKEGLEKELK
                     IPIVLTSARNGQGMKELKDLIEKTSQIIEEETEKSLYFNQDESIREKISEDNYNYANK
                     LKQKYVKEDVNKLNRDKKIDDIVTSKKFGIPIMLALLGLIFWITIQGANIPSQILADL
                     LFGLQYKLDKILLILGVNDFIRGVFIDGMYKSLAWVISVMLPPMAIFFPLFTLFEDLG
                     YLPRVAFNLDHLFKRAGAHGKMCLSMCMGFGCNAAGVIGCRIIDSPRERLIAVITNNL
                     VPCNGRFPLIIVLSAAFFAFTGSFVDSIIPALSITLIVLIGVFATLSVSYLLSNTLLK
                     GIPSTFTLELPPYRAPQVGRIIYTSLIDRTIFVLSRAAVVAAPAGAIIWLLANIYVGE
                     LSILIHMANFFEPLGRAIGLDGFILIAFIAGFPANEIVIPVLLMAYMSTGTLTDFESI
                     DSLRQILISNGWTQLTAINVMLFSLLHWPCSTTLLTIKKETGSLKWTLAAFIIPTGIA
                     FLVCFITANLYRIIW"
     gene            complement(16133..16351)
                     /locus_tag="PLB33_02105"
     CDS             complement(16133..16351)
                     /locus_tag="PLB33_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG62344.1"
                     /translation="MKLADVGVGKTVKVKQNSAHDYLKERFLALGLTKGADVEVLRKG
                     PKNNLTVYRIRGAMIALRNEESSLIEVL"
     gene            16476..16907
                     /locus_tag="PLB33_02110"
     CDS             16476..16907
                     /locus_tag="PLB33_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOG62345.1"
                     /translation="MKEEFYTLKGYKIKNEEILSSSMEDYIEMICRLSEKSDEVRVSE
                     LSSALNVQPPSTTKMIRRLAKENYVTYERYGFIKLTEKGREVGSKLLERHRIIFEFLE
                     CIGVKENILEQTEIIEHAVNDEVLNKIDELTQFLKKCDKYK"
     gene            complement(17016..17144)
                     /locus_tag="PLB33_02115"
     CDS             complement(17016..17144)
                     /locus_tag="PLB33_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HOG62346.1"
                     /translation="MFKKLTNKLKMRYRRSANPGNVNIPGEFEVQERKKDVDNNKK"
     gene            17283..18056
                     /locus_tag="PLB33_02120"
     CDS             17283..18056
                     /locus_tag="PLB33_02120"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62347.1"
                     /translation="MTEVDMVYICELRNVSKRFHTINNETKAIDDISFKVKKGEIVSI
                     VGPSGSGKSTVLSLIAGLEKPSEGEVIVNTDSIGFMFQKDQLYEWRNVYKNCLLGLEV
                     KKMDNEENKIKVKRMLEKYGLTNFKNHYPSQLSGGMRQRAALVRTLAIEPELLLLDEP
                     FSALDYQTRLSIGDEVGTILRNEKISTILVTHDIPEAVSISDRVIVFTERPAKIKKEY
                     EIKFEECPGERTPMKCRNTLAFGEYFRNIWRDLDVGKEV"
     gene            18037..18855
                     /locus_tag="PLB33_02125"
     CDS             18037..18855
                     /locus_tag="PLB33_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62348.1"
                     /translation="MWEKKFEPVANISEEHKEYLKLINREKNFIRIAQILIFVFGIIF
                     WEVAARKNLIDTFLTSSPGAILGLIMKLVDDGSLFKHLFISTMETIGGFFAGTVGGLL
                     IAIILWWFRKLAKVMDPYIVILNSLPKTALAPIIILWVGMGYSGIIATAASISIVVTT
                     MTLYTGFSNVEKDKIILLKTMGANKFQILKKVIIPANIPNILSVIKVNIGLSWIGVIV
                     GEFLVSKAGIGYLILYGSQTFKLDLVMMGVFVLGVIAAIMYVFSSWLEKKIIKY"
     gene            19092..19643
                     /locus_tag="PLB33_02130"
     CDS             19092..19643
                     /locus_tag="PLB33_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62349.1"
                     /translation="MAAVSSIFYPLIFLVLIYYLFKNTKNRMQYKKENMEALLFLDED
                     DKRVHLMSRLLMALMILISGIMIRGLIETKTYAAEQVLIMGVLPILIITLYIPLSKKT
                     MISTLGIHKRGNLIRWEDIKGINYPKPDEKGKVKVNIIYSVMNKDSNIHLTFLKNDPQ
                     LDKFKEHAKTYRNTKKKDKKSGK"
     gene            19633..20430
                     /locus_tag="PLB33_02135"
     CDS             19633..20430
                     /locus_tag="PLB33_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOG62350.1"
                     /translation="MVSKFIDSHAHLDDKIFETDRDNLISSLYDNDVEAVLNPGADLQ
                     SSKEALLMANKYSFIYAAVGCHPHDTKYMSDEHMNEFIEMAKNKKVIGIGEIGLDYYY
                     DNSDRDTQRKWFREQIRLAKELNLPYIVHDRDAHEDILRIMKEEHYDGARGILHCYSG
                     SVELSKEFIKLGFYISLAGPVTYKNARVPKLVAKEVPFDKLLIETDSPYLTPVPYRGK
                     RNEPKYVKYVAEEIARIRNVSIDEVAEKTKENFKRLFNIEEGGHTFK"
     gene            20480..21076
                     /locus_tag="PLB33_02140"
     CDS             20480..21076
                     /locus_tag="PLB33_02140"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOG62351.1"
                     /translation="MKLNRNFYNRDARIVGEDLIGKILVRKYNNTVIKARIVETEAYI
                     AAIDKASHGYGGKITPRTKIMFGPPGYAYVYFIYGMYYCLNFVTEEEGTCSAVLIRAV
                     EPVINKEEMCFNRYKEHLDDITKYQKKNISNGPGKLCNALKITIEHNGTDLTGNEIYV
                     EDDGFKDFKVEKSKRINIDYAEEAKDFLWRYTIHHKKA"
     gene            21131..21586
                     /locus_tag="PLB33_02145"
     CDS             21131..21586
                     /locus_tag="PLB33_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOG62352.1"
                     /translation="MSNHETVNEILVRLFANILYIEEKCLKIGEFKDLTITEMHLIEN
                     IGYSTERTMSDTAKAVKVTSGTLTTAIDNLIKKGYVERRRSSEDRRVVKIRLTEKGKA
                     AYKSHEDFHKDLVISALQQLNSEEEEVLIRVLTSIEKFFSEKYKYAGWY"
     gene            21625..22467
                     /locus_tag="PLB33_02150"
     CDS             21625..22467
                     /locus_tag="PLB33_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG62353.1"
                     /translation="MGIRIVIDSTSDVTDEIIKKYNIKMVPLTVNFENESFLDKVELN
                     SKQFFEKLETAEKLPTTTLVSPGTFMEVFSDILLEGDKVLGLFLASEFSGTYDSACMA
                     KNMIGSDDIYIIDSRSVCLGAFALILEAIELVQQNKDIEEIVKELEALREKVVAVAAL
                     DTLKYLEKGGRLSKGQAVVGTLLNVKPIVEIKDGKISVIEKIRGKNKAVKWFDEWIAK
                     NNYDLSNKTVLLFHGKAYEQLKMLRESIEDKYKIKNIIEQEIGAVIGTHSGPGVLGIG
                     FVNK"
     gene            complement(22610..24931)
                     /locus_tag="PLB33_02155"
     CDS             complement(22610..24931)
                     /locus_tag="PLB33_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG62354.1"
                     /translation="MKNMLCSICKKNVAVIYVNKIVEGKMQTTGLCIPCAKKQGIAPM
                     DQIMRQSGMKPEDFDNINKQLTEIIGDMDLEKLSGDMSESSDGINSLMNLMNNAFTGN
                     TSSTSNEFIPEDEDADYTGPGSGTGPKIKEKVKRKKLKYLDTYGLNLTEQARFGELDR
                     IIGREREIDRVVQILNRRSKNNPVLIGEPGVGKTAIAEGLAVRIAEKKVPVKLFNTEI
                     YLLDLTAVVAGTQFRGQFEARMKAIIDEAKKAGNIVIVIDELHNIVGAGEAEGGAMNA
                     ANILKPALAKGDVQIIGATTLDEYRKHIEKDTALERRFQPVIVDEPSIEDSIEIIKGI
                     KDYYEQFHNVKITDEIIRQAVLMSERYITDRYLPDKAIDVIDEAGSRANLKNKGLVEL
                     EALKAELSRIRDEKEEAAENDDYERAAELKVQEIRIESKLKELSNEYENILLTEEDIA
                     YVIEAWTKIPVQRISEAEADKLIHLEERLHRRVVGQHPAVTSLAKAIRRNRSGFKKKK
                     KPSSFIFVGPTGVGKTELARTLAYELFENEDAMIRLDMSEYMEKHTVSKLIGAPPGYV
                     GFDQAGFLTEKVRRKPYSVILLDEIEKAHEDVFNILLQILEDGRLTDNQGRTVNFENT
                     VIIMTSNAGTSIKANRIGFGNTEYEAMESKVKDALKQAFRPEFLNRIDDIIVFSELTK
                     EELSQIVDLMLKDVIKEAEEKKITLTVTDKMKEHILEKGYNTKYGARPLRRAIQKYVE
                     DEISEAYLKGVFKENSLVTVDVNDEGKTVLTII"
     regulatory      25173..25323
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            25496..26392
                     /gene="dapA"
                     /locus_tag="PLB33_02160"
     CDS             25496..26392
                     /gene="dapA"
                     /locus_tag="PLB33_02160"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOG62355.1"
                     /translation="MNDLVRGCGVALVTPFADGEIDYEVLGKLVEMHVENSGSIIVAG
                     TTSEAATMTDEEYHTVVEFVIDRVNKRIPVIAGAGSNCTQAAVKKAQFCEEAGADGLL
                     IVTPYYNKTTQRGLVNHYKIIAESTSLPIIVYNVPARTGMNIEPKTVLELSKIKNIVG
                     IKEASGDISQIAEIIRICPDDFLVYSGNDDMVVPLMSLGGHGVISVSANILPAEMKKM
                     VDDYRNGNVNDAKNAQLALNGINKALFIETNPIPVKEAMSIMNLCSSEVRAPLYQMSE
                     ENRARLLRELNLFGIGVPLETA"
     gene            26401..27069
                     /locus_tag="PLB33_02165"
     CDS             26401..27069
                     /locus_tag="PLB33_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG62356.1"
                     /translation="MNVVICGINGAMGRVLQEEIKTYDNINLIGSLSPRSGRFGQDID
                     GMPDVVIDFSNTANIDFLIEYATKNKCALLICTTGFSDEDKAKIKEAGKAIPVLLSSN
                     TSLGINVFRKILKSISAELSTFDINIVEKHHTKKIDAPSGTAKTLSEDIKNATGREHV
                     EISAIRAGTIPGEHTVIFSGVDEILEIKHTAFSKKIFARGAINLAMKLSSKSPGYYLM
                     EDFF"
     gene            27096..28079
                     /locus_tag="PLB33_02170"
     CDS             27096..28079
                     /locus_tag="PLB33_02170"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOG62357.1"
                     /translation="MNKIRIGIVGYGNIARGVEAAVNLSTDMELVAVFTRRQPDTIKT
                     QTDVKVLSVDEVEKYKDIIDVMVLCLGSATDLPVQAAEYAKMFNTVDSFDNHKKIPEY
                     LRKVDEGALSSGKISIISIGWDPGLFSIMRMLSESVLPEGSSYTFWGRGVSQGHSDAI
                     RRIPFVKQAIQYTIPVEDAVNCVRRGDNPELTAREKHIRQCYVVCDECADKNKIEEDI
                     KNMPDYFAEYNTEVNFISEEEFKKNHSRMSHGGLVIRNGMTGSEKNQHIMEFSLKLDS
                     NPEFTGSVILSYARAAYRMNKEGIKGAKTIFDVPLSYIHPEGREKLIRNFL"
     gene            28175..29497
                     /locus_tag="PLB33_02175"
     CDS             28175..29497
                     /locus_tag="PLB33_02175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOG62358.1"
                     /translation="MLKVAKFGGTSLADSKQFNKVYDIIKNNDERKYIIVSAPGKRFK
                     DDNKITDLLYLTYAHIKYSVPYAPILKIIEDRFRQIKEELNLKTDLDYEFKIIKENLD
                     NKCEEDYIVSRGEYLCALLMADYLDCDFIDAKDVIFFNYDGTVNEEKTHEKLTEILGK
                     SNKAIIPGFYGSYPDGSIKTFSRGGSDITGSIVSSVMGADIYENWTDVSGFLVADPRI
                     VNNPKQIKKITYQELRELSYMGASVLHEEAVFPVRQAGIPINIKNTNDPENPGTLIIR
                     DDQAGEDYANGHIITGIAGRKDFSFFYIHKEHMAHEVGIVKKALEVFEERGISIDHIP
                     SGIDSFTIVLPSESVEKISHQIVEELKKKCKTDNVQVFRNLSLITTVGIKMAYRPGIS
                     AKLFTALGNRNINIRMINQGSSEINIIVAVEDKDFEEAIRAIYDAFIK"
     gene            complement(29580..30113)
                     /locus_tag="PLB33_02180"
     CDS             complement(29580..30113)
                     /locus_tag="PLB33_02180"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOG62359.1"
                     /translation="MDFFMNLEGPLLYFIIFFAKIIEVSISTVRIVFINKGEKIKGAL
                     LGFIEIMIWLIVVSSVLNNITEDPIKVFIYAIAFSLGNFFGVTIESKIAVGLSSIQVV
                     VDQKEGEVLADILREQGFGVTILEGKGKNDSVKNLLFIQLKRKKIPVATKLIKEHNPE
                     AYITVNEIKTMFGGYIK"
     assembly_gap    30330..30429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            30609..31253
                     /gene="trmB"
                     /locus_tag="PLB33_02185"
     CDS             30609..31253
                     /gene="trmB"
                     /locus_tag="PLB33_02185"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOG62360.1"
                     /translation="MRLRYVPGQYQYLKNCEKVITEPEKQGFKLNKVFNNNNPIHVEL
                     GCGKGKFLRENAINNPHINYFGFEKSVKVAYRCAKSAFENDPPNYYIVYSNGDILKEV
                     FDAGSIDRIYLNFSDPWPKPSHYKRRLTHKNFLDVYKIVLSKNGEIHMKTDNNDLFEY
                     SLEQFEKDNWEFKMVTRDLHNSIHKEDNIMTEYEERFVNKGIKINKLIVRRKNG"
     gene            31246..31518
                     /locus_tag="PLB33_02190"
     CDS             31246..31518
                     /locus_tag="PLB33_02190"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOG62361.1"
                     /translation="MVNIKYIMFYLLIINLISFALFFADKQKSKRDKWRIKESTLHLV
                     SFMGGTLGSIAAMILFHHKTKKPKFVIITIIALLFNVVLVYELLIR"
     gene            complement(31595..32338)
                     /locus_tag="PLB33_02195"
     CDS             complement(31595..32338)
                     /locus_tag="PLB33_02195"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOG62362.1"
                     /translation="MKKLDGRVAIVTGSTSGMGRATAVLFAREGAKVVVTGRNEERAK
                     AVVDQIKGEGNEAIYVLVDTSKVEDVKKLVDVTLDTYGTVDILFNNAGALSMSPITDV
                     SLEEWNKIFNVNVTSALYLTQLVAPIMKAKGKGVIINTSSVAGFGAHHGFAAYVSSKH
                     AMNGLTKSMAWELGPEIRVNGIAPGLIHTAMVDSIGGPSVVQHMIDQSPVKRVGLPED
                     IANAALFLASDDSSFIDGQIIKVDGGFEI"
     gene            32575..33153
                     /locus_tag="PLB33_02200"
     CDS             32575..33153
                     /locus_tag="PLB33_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HOG62363.1"
                     /translation="MEYRNANINDIYGVSQLQKKYHIYTISEEDKPDGFVTTLFTEEQ
                     FKELIEKENGLAIACDNDKVIAYAMAASWDYWSAWPLFQYMINDLPNIQYLGYTLSTE
                     NSYQYGPICIDKQYRGTEVLPNLFEFSRLQMKDKYPVLITFINQINGRSYRAHVDKLG
                     LEVIKTFDFNNNHYYELGYDMRKKTPGANLYK"
     gene            33632..34354
                     /gene="cas6"
                     /locus_tag="PLB33_02205"
     CDS             33632..34354
                     /gene="cas6"
                     /locus_tag="PLB33_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HOG62364.1"
                     /translation="MRISAVFSLEQNSIDKDKNRIFISILKNILESYDVNVYKKFFES
                     GPVRKSYCFALYMKDAEFTRDKIIIPYKKIVLKLSTADLEDAIHIYNAFLQNIRRTFK
                     IRENELKLDKISIEREKKIYDDNVIFISLSPILIREHEGDNKKTWYHTLNDEKGRMLF
                     LNNLKIQLLEEFGRDRILDIEEVKVNVLSNKNVKVKNYSIEVAGNLCTFEMHAKSYIL
                     EHVYKAGIGSQKSTGFGMLDLL"
     gene            34366..>35936
                     /locus_tag="PLB33_02210"
     CDS             34366..>35936
                     /locus_tag="PLB33_02210"
                     /inference="COORDINATES: protein motif:HMM:NF021241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cas8a1 family CRISPR/Cas system-associated
                     protein"
                     /protein_id="HOG62365.1"
                     /translation="MSEKVRLYLDDWLFNSGLVGFYNILKKSEDTITVGENYLEFNIE
                     ALNNFDEKYFEYFISAYEKNTSWYKIVSYEDVINHYKQNNFEEFNKKDIDNLNKYIND
                     VKKFIKSNSYQAVFEFLGLQKDISSLEKQLTAIKLKDGQDTRDVFPDIKNTFNILGKI
                     IEFINKNNVKKYVKAKNVIYTIINNGWNGVCFLNPQTKEKDMYKDYENYFVKPAFEYF
                     NEDKSTYKYECFSCDSKMKNMKDNLGFLNSIGFDVNKKSSHVWNFNNDVAVCPLCKLI
                     YSCVPAGFSYAIDSGIYVNDNFSMQNAIDVNNKIKKEILKTNENKRALTYRALVESIK
                     EQFSMSAKYELSDVQVVRYVNEGYRFNILTRNILELVYECREELNSLISCWYKEIKTY
                     FNIYDMVLDNLFNSQNLYLLIHKMLLYKLSDFNNCYYNWMQTVNVMEINFKFMRRLGY
                     MESCKNDIVTISNKSGYFLREAYKQKDPNTSKLSGISYRLLNALKVNDKDMFMDTILN
                     CYLYVKKSVPPVLLE"
BASE COUNT        12559 a         5574 c         6659 g        10944 t
ORIGIN      
        1 ggcgaagata cggcttcttc agttattgac ggaacagaaa tttatctgcc cttagctgat
       61 ttaatagatt atgaaaaaga aatcgaaaga cttgaaaaag aaaaaacaag gcttgaaggc
      121 gaacttaaaa gagttaacga caagttaaat aatgaaagat ttatgagcaa ggctccggaa
      181 aatgttgtta atgaagaaaa agaaaaactt caaaaatatc agctgatgat ggaaaaagta
      241 cttgaaagat tagagcaaat gaaaaaataa agatgagata cttcatgtta ttaataatat
      301 ctttgtcatt ctgagggctt tagcccgaag aatctcatga gatccttcgc ttacgctcag
      361 gatgacaaaa cgtattaata tcgtggtgtg gtaaacattt gataattctg agagtcaaga
      421 atctaattac taaaaagatg aaagaatgac aagattattt ataacaataa tgtatgataa
      481 aaggagacat gaacatggat tataacgaat tattaaacaa agcaagagaa aaaatggcac
      541 caagatgcag ggtttgcaag gaatgcaacg gggtagtatg caaaggtgaa gtgcccggtg
      601 ttggaggtaa aggcagcggt aatgcattta aggtttgtat tgattttctg gcttctgtta
      661 aaattaatat ggacactatt tacaaaaatg aaggacaaga cacttctatt tcattgtttg
      721 gaaagaattt taaatatcca ttctttgcag ctcccatcgg aggcatggtt ttaaattaca
      781 acggttccat gacagaagca gaatatgccg aagcaattgt taacggtaca ctaaaggcag
      841 gatgtgccgg gtttacagga gatggaccta tggataattt attcttggac agcctgccaa
      901 taataaagaa agcaaatgga attgcagtac ctactattaa accatggaaa aatgacaagg
      961 ctttggaaaa aataaaaatg attgaagaag taaatgctat ggcatttgca atggacattg
     1021 attctgcagg ccttataaat ttagcattgg caggcaagcc tgtttctgca aagagtgttg
     1081 aagagcttaa agaattaaca gatgcaacca atcttccttt catcattaaa ggagtaatga
     1141 cagctaaagg agcggaaaag gcttatcagg caggcgccta tggtattgtg gtatcttctc
     1201 acggaggaag ggtattagct gatgcgccgg ctacatgcgc catgctccct gaaataagag
     1261 aagcagtggg agataagctt aagattttcg ttgacggagg aattcgctcg ggagctgatg
     1321 tatttaaagc aatagcattg ggagcagatg cagtattaat cggaagacca tatgccattg
     1381 ctgcttttgg aggcggtata gaaggtgtgg aaatgtatac cgaaaaaatc ggtgctgaat
     1441 taagagaaac aatgataatg accggttgca aaaacttaag tgatataaca agagataaaa
     1501 ttattttata aaccaaagta aaagataata aaaatgtgca aaacttagtc aagtcttgca
     1561 catttttaat attgtaatta attttaatta gtgaatattt gtgtccaata tggtcttccg
     1621 cttgagtttg ttgcatatcc aactccaatt tttgagaagt tagatgataa gatgtttgct
     1681 ctgtgtccgg gtgaattcat ccatgcatct accactatct caggagttct ttgaccggct
     1741 gcaatgtttt caccccatgc acgccagctg attccaaact gtcttaacat atcgcccgca
     1801 ctgccataag taggtgactg gtgggcaaaa taattgttta ctgccatatc tttagattta
     1861 gttcttgcca cgtttgaaat tgctgaatcc atttttaatg ccggtaagcc tgctttttgt
     1921 ctttcaatat ttacaagttc tactactttt tgttcgtatg ttaaatttga cgaaggaact
     1981 cctgtattat tttcaacagg tgccggagcc ggagctggag ctggtttttc aaccggtgct
     2041 ggagctggag ctggcttttc aaccggagcc ggttttggag ccggtttttc aacaggtgcc
     2101 ggagccggag atggtttttc aacaggtgct gccggttttt gtgccggtgc atattgtttt
     2161 aaaaccgttt tccagtcaat tgattttaaa tctactgttt ttccgttaac agtaatttta
     2221 tactgtgtgt ttttgttagc atagttgctt gtgttagttt tgataaaatc tttaagatta
     2281 tacttataat ttaccgatgt gcaatttgtt gttgcagcaa atgctgatgt tgatgataac
     2341 gctattacta acgctaatgt taatgctata attcttcttt tcataattta ctcctatata
     2401 gtctaagatt tttttcgaaa caggtaaggc ctgttccttt gaaaagctag aacttgttaa
     2461 tgagttttcg ccatatcaga attatagcac tgcaattccc ctaaaaaaac aatcaataac
     2521 ttcttataat gccaaatgtg tcaataatat tacactattt tacaaacaaa agaaataaat
     2581 acataattat gacaaatata tatagtttat tcaaaaatac ttaataaaag gttaattatt
     2641 agggcattat ttgtaagggt tgtatttaat taacaaattt acttcttgac aacacttaaa
     2701 ttttatatta ttattccaat aatactttat catgactgtg aaggaactta agtaacattt
     2761 tggttacaat ttagagagtg tatcgggtgg tgaaagatac acagcacaaa gtgtgaaata
     2821 cgattccgga gttctgtgtg aaaagcacag cggcttatct gtccgttaaa tcagcaagag
     2881 gcttaggcaa attaaggtgg taccacgggt tatctcgtcc tttcggatga aataaccctt
     2941 atttagttag gagggcaata gatggaaaaa ggaaatgttt ttgatgtatt agttgaaaga
     3001 gggtatgtgg agcaatgcac tcatgaagaa gaaataagag aattattggg aaaagaatcc
     3061 gtaacatttt atataggatt tgacccgaca gcggacagtc ttcacatagg ccactttata
     3121 caaataatgg taatgtcaat aatgcagaaa tacgggcaca gacctatagc attactaggt
     3181 gacgggacaa caatgatagg agaccccagt gacagaaccg gaatgcgctc ggttatgtca
     3241 tttgaaacca taaccaaaaa tgcagaaaac tttaaaaagg tatttgagaa atttcttgat
     3301 tttacggatg gaaaagccct gatgccaaga aattccgaat ggcttttacc tttgaattac
     3361 cttgaattca tgagggaagt gggtgtacat tttacaatca gcaaaatgat tgcagcagat
     3421 tgctataaaa atcgaatgga ccagggtctt acattctttg aattcggcta tatgcttatg
     3481 caggcttacg acttctatat tttaaatgaa aaatacgact gcaagatgca attgggcgga
     3541 aacgaccaat ggtcaaatat aatagccggt gttgaactga caagaaaaaa agcaggcaaa
     3601 caggtttatg gcatgacatt tgccttattg acaaacagcg aaggcaaaaa aatggggaaa
     3661 accgaaaaag gagcattgtg gcttgacagg aataaaacca caccttatga attctatcag
     3721 tactggagaa acattgatga tgccgatgtg gagaaatgct tggcacttct tacattctta
     3781 cctatggatg aagtaagaag attgggagca ttaaaaggcg ctgaaataaa taaagctaaa
     3841 gagattcttg catttgaaat aactaagatg attcatacgg aagaagatgc agttaatgca
     3901 caggaagctg caagagcttt atttggaggc ggagctgatt cagaaaatat cccgactaca
     3961 gaattaacaa aagcagaact gggtgaaagc atatctgtta ttgacttaat gcttaaggca
     4021 ggattaataa aatcaaaaag cgaaggaaga aggcttatcg agcaggccgg agttgctgta
     4081 aatgatgcaa ttatagatga tataggtgca acagttacgg aagctgactt tgaagattca
     4141 aagctgatga ttaaaaaagg caagaaaaca taccaccgaa ttaaattgtc atgatctata
     4201 ctgtttccat tgaggacggt ccttttcatt acaaagggac cgcctcagtg tttgaatacg
     4261 ataatacaat aggaggcatg ttctttgata aatttatttg aaatagattt aaatatgata
     4321 gcttcatttg ctattgtgtt tggaattata atttatgtag gatatgtaaa tacatcgata
     4381 agagagttaa aaaaaagagt tgaagaattg gaaaataaaa ttggtataga taagaaagcg
     4441 gacgaagaac taaaataaga atgatataag atcttccacg actgtcgggg tgatgattag
     4501 gtatcctaag gctgattgga ggatcttttt taatttcttt tatatataat tattaatagt
     4561 tttaggaaag tatggtaaaa taatctcata ttagacgaaa aggagtgatt atgtgttaag
     4621 tagaaaactg attgaaaata cgcttacggc tgctttatct accggcggtg atttcgccga
     4681 aatttttgta gaagaccgaa ctaacaacgg tattgttatg ataggcggca aggtggaaag
     4741 caccatgtcc ggaagagatt atggcgtagg tatcagaata ttcaaaggat ttaacagtgt
     4801 ttatgcatat acgaataagt ctgatgaaga cactttaata gaaacagcta aaaaagctgc
     4861 tcaagccgtg gaaggaaaca tcattgactt aacaataaat ctgacaaagt cagatgttaa
     4921 aaatataaat atcatacaaa tagacccgtc caaggtcgaa aaaactaaaa aggttcaagt
     4981 aatgaaaaag gcatatgaca ttgcttttaa ttatgatgaa ctgattacac aggtttctat
     5041 aaattacacc gattatatac aaaatataat ggtggctaat tctgaaggat tgtggaagga
     5101 agatacgagg gttcgctcaa gactcgggat ttcttccgta gcaagtaaag acggtgaaat
     5161 gcagtcagga tttttcgcac cgggagcaag caaaggctac gaattttttg aaaatcttga
     5221 tatagattat tatgccaagg aagcttcaag aatagcggta acaatggtta aagccaagta
     5281 cagtcccagc ggcaggatgc ctgtaattat cgataacgga tttggaggag ttattttcca
     5341 cgaagcatgc ggtcacggat tagaagcaac atcggttgca aaaggccttt ccgtatttgc
     5401 cggaaaaatc ggacagcaaa ttgcatcacc tatagttaca gccatagatg acggatcaat
     5461 tcccaatgag tggggctcat tcagcataga tgatgaagga actcaatcac agcgaaatgt
     5521 gctcatagaa aacgggatac ttaaaggtta catgatagat aaattaaatg caagaagaat
     5581 gaatatggat ataacaggct cctcaaggcg tcaatcctac agattcccgc cgacatcacg
     5641 aatgtcaaat acatttatag ataacggcag ttcaacgcct gaagaaataa ttgctaatac
     5701 ggaaaagggg ctttatgcaa aacatatggg cggaggctcg gttaaccctg ccaccggaga
     5761 atttaacttt gcagtaatgg aaggatacat aattgaaaac ggtaaaataa aagagcctgt
     5821 aaggggagct acgctgatag gaaaaggcat ggaagtactt aataaaattg atatggtagg
     5881 aaacaatctg cttcaaagcc agggtatgtg cggttccgta agcggaagca taccggcaaa
     5941 tgtaggccag cctatgatta gggtttcgga attaaccgta ggcggaaggg aaggtgagta
     6001 ataatggaaa tgaaatcatt gattgataaa atatttcaaa aaggcagaga aaagggcctg
     6061 aatgacatgg aagtttatta ttctgcagga agtagcctta cattgaaggt ttttcagaaa
     6121 gagcttgaaa cgtataattt gtcggaaagc gaaggacttt ccttaagagg ggtttacaag
     6181 ggtaaaatgg gatattcata taccgaaaag gtagatgaaa cttcaataga ccaactggtt
     6241 agagatgttt tggaaaatgc cgtcataata gattcggacg atgaagagtt tatatttgaa
     6301 ggctcgaagg agtatataaa agtagattca ttcaatccgg ctttgtcaaa tgtaagtgaa
     6361 gaaaagaaaa ttgaatttgt taaaagtctt gaagaagaag catttaaatt agacaaaaga
     6421 attgaatctg ttgaagtatg cgtttacggc gacggttacg gagaaataat aatgtccaat
     6481 acaaagggtc ttttcctcca agataaaagc aatattgcat atacctacat agttgttgtt
     6541 gcaaaagaag gtgaagacat aaagacgggt atggcatacc gtacagggaa tgattttaat
     6601 aaatttaatg cccgtgatat tgcaaaggaa gccgtagatg aagcattggc tcttcttggg
     6661 gcaaaatcgg taaaatcagg agattacaat gtgattatca gaaattccgc tgcagctgat
     6721 ttattggaag catttactgg aattttctca gctgaagcag tgcaaaagaa tctttcctta
     6781 ttaaaaggaa aattaaatga aaaaatagca agcgacaaat tcactttgat agacgaccct
     6841 tatatggaaa gcggtcttgc atccaaatcc tttgacggag aaggggttgc atgcaaatat
     6901 aaaaaggttg ttgataaagg tgttttaaaa acttattttc ataatttaaa aactgccaaa
     6961 aaagacggcg ttgaaacaac aggaaatgca agcaagggct cctataaatc gtcattaggc
     7021 atatctcctt caaactttta tgtagaaaag ggagaaagga gattaaatga aatgattaca
     7081 gacataaaca aaggaatact catcactgaa cttcagggtc tgcattcagg cttaaatacc
     7141 gtatcgggag atttttcatt ggcagcattg ggttttttaa ttgaaaacgg aaaaatttca
     7201 agacctgtgg agcaaattac agttgcggga aactactttg aaatgcttaa aaatatagaa
     7261 gaaacaggct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttagtcaa
     7381 ctctttcacg ttcattaaat aataatgtaa tacagtacaa tcctgccaac cctaccagcc
     7441 cgtataccaa tcggctgaat aatgctgtct ggccgccaaa aatagcagct accaggtcaa
     7501 actgaaataa tgcaatcagt ccccaattta ttgctccgat aattactaaa aacaatgcaa
     7561 atttatccaa ttattattat cctcctcatt tatgaatata aaattattat ttcaaaattt
     7621 ttctatatta tgcatatttt caaaaaattc aataagacaa taatcattaa cataaatatt
     7681 tcaaatgaat ataataaatt atcgtaatat tcgtaattgg gaggtaaata tggagtgtaa
     7741 taaacctgaa aatattgtag aagcaaaagc tgatgtaaac acacaaggcg attgcgtacg
     7801 cggcgatagg ggaaggagag actgtataag aagagacaga gaccgtgtta tgggaacagg
     7861 cggaattgat tcatcattgt tgtttttctt tttgttatta gttattattg tggatcagag
     7921 cgattgtgat attgaatttg atactttatt gtggttcttc cttgtgttgg ttgcaatatt
     7981 ctgtaaatgt gactaatccg aagtttatta ttttagtcaa agaaatgggg ttaggtcata
     8041 ataggaataa aaaagacctg cctgataatt caggcaggat tacttttttt atgagaatat
     8101 cggagacact ataaaatcct tgaagaggag accgccataa tcaatcctta cacccttcaa
     8161 cccaacttca cagtccttgg atactatgat atctaaaccg tctatattat ataagttatc
     8221 atcttccgtt tgtttcgccg aaacaatttt atatttaaag cctgctcatc ccaccgggta
     8281 tttcatcaaa gttatgggca tatttttttc cttcatgagc aagagctttt cttttgcact
     8341 gtctgttatt tctatattca taaatcctcc tcgctttttg cttaatctta tatgattaaa
     8401 aaaaataatc agtgatttaa tcacacataa ttttgagact ttatctgcca aaaaagtata
     8461 tatatcaata cagccgattt cctaatggtt aagaagcaga ttttttattt cttctttatt
     8521 atttgcataa ttctttacaa ttaatctgtc aataatatta acaggtctgt cttttaaagt
     8581 gtaaataata tgagataaat caacggcttc ttcaacatcg tgggttataa agattaccgt
     8641 tctttggctg aggttccaca tttttaaaaa taaagagctc atttcgcctt ttaatttatt
     8701 gtcaagacct ttaaacggtt catccaatat aagtatatga gaaggatagg caaaagctct
     8761 tgccagagat accctttgcg ccattccgcc gcttaatgca tctattttat aatttctgta
     8821 ttcgcttaat tcaaccattt ccaaataact gtttataatt tccgcccttt tgtcaggttt
     8881 ataaacatcc tttaatacaa aatcaatatt ttcatataca ttgtaccatt taagaagacg
     8941 cggctcctga aaaacataag aaaacaatga attgtcaata ccgacaacac gaccctcatc
     9001 aacaggtaca attcctgcca caatatttgc aattgtagtt tttcctacac ctgaaggacc
     9061 aaacaagcat gttattttat ttttttcaaa atcaatgtta aaatctttca ataatataag
     9121 gttatcatac tttttagaaa tattaattaa tttaatcata tttatctccg aatttgaaaa
     9181 tggttttaag caggctttca aacagaaaac ttagtgaaac tataataatt gtccaggcaa
     9241 acaagtcggg aatttcaaga tatacctttg aatcgtacaa aaatctacct attgaatatt
     9301 tgggaagact tagaacttct gctgcggaag taactttcca gcctattccg agtgcagaaa
     9361 tcatccctgc cttaatataa gggagagaag aataaaaata tactgaactt attatcctcg
     9421 acttttttat tttataaact tcgcacattt gaagaagctt aacatcagtt gataggatac
     9481 ctgaaaccgt attagtccaa attataggga agcacattaa gaatgcaaca aaaatgggaa
     9541 cattggtatc cttgaaccac ataattgcaa taattataat actcattacg gggattgttc
     9601 gaataattac tatcaaaggg ttaagcaaat tgtatacagt cttatgaatg ccgcttataa
     9661 ttcctaaaaa tatgccaaag acacaggata aaaaaaatcc ggtcaatgtc ctgtaagtgc
     9721 tgtaatatat agtaatgtaa gtatcctttg aaatcaacaa attcatgaga gaagaaaacg
     9781 ctgcatgagg agaagggaga tatatctcct gagctattat cagcgatagt atctcccaaa
     9841 cgaaaatcca aaaaagtata atcaataatt tgttcttatt ccttaaaata gaattcatca
     9901 tcaggcatgc ttcctcctac cgaagcaggg tttgaatcaa ataatatttg ataaaaatta
     9961 tttaagcttt cttttgcttc tgcagctttt ctaaatgtta tgccgcagta aggaagagct
    10021 ttttcaacca atacggcatc aggcataatt tcatgttttg cggttaatac agatgcttcc
    10081 ttttgattct ccaataccca gttaaccgag ttttcatatt gtttcaggaa ttcatctaca
    10141 aattccggat tattttctgc aaactctttg tttattataa tacatcccgt aaataattcg
    10201 ctttttccgc ccgttgcatc attccatgct tcatttaaat ctataagcat tttaacgttg
    10261 ggatttttca gagtagtttg agttacaaaa ggctgaggca aaagcgcaac ccttgtatct
    10321 tctgctataa cggactgtgc aagagttgca tggtcaagtg cataatttaa ctctgtttta
    10381 ccttcaagac ctgcttgctt taaaatataa ttcatggcaa aatcaggagt tgaacctttt
    10441 gcgctcattc caagcgtctt gcattccaat tgtgaaacgg agcttatatc ctcggttcct
    10501 actatatata gatttccaat cgtatttacg gctaacaact gaacctttcc ctgagtttta
    10561 ttgtataaaa ccgatgctaa atttatagga acagatgata tttgaatatc gccgtttata
    10621 attttacccg taagtgaatc aggtgctgat tcctggatat attctacatt gtatttatcg
    10681 cttttaagtg ccttttcatc aatcatttta atcataccaa ttgatgtggg acctttaagc
    10741 cctgcaatag ttacatttac cggttcagat gccggctctt caggcatggg tgtttcttct
    10801 gcattttcat cgggtgtcgg tgtctctgca ggttcattgt tgcagcctgc cgtaaaaatt
    10861 aacacaataa gtaccaccaa caaaaaacta atttttttca tattacctcc taatgttttt
    10921 ggacaaagca cttttgacgc tgagtccttt tattttgcct aattttccgc taagagctcc
    10981 tatatcatcg ttagtgccgt caactataat agagataaca ttaagacctt tctccctgta
    11041 aggtatgccc attctgccta taataatatg agaatactca tgcaggatac ggttgacttc
    11101 tgcaatttgg gatgtatctt caattacaat gcctataact ccgattcttt tatccatatg
    11161 cacctcaata aaaaattcct tgcacaacaa ggaataataa ttaatactat aagtgtattc
    11221 cttgctctta gaagatcctt aagcttagat tatgttatta ttttaacata taataataat
    11281 tatttcaaca ttattattgt taagattatt taatcaattc aattagttct tcctttgaaa
    11341 tattgaaata ttgtacaaaa cttgacaaat aaaaatcaga aggtatttct tctcccgttt
    11401 cataaagttt tattctttct tttggtacat tcaatgcgtt tgcaagttct tgttttgtta
    11461 aattgttttc ttttctgatt tgccttaatc tttcgcctaa catattatac ccctctcaat
    11521 attgagctta attaattata acatatactt tagaaaggta aagttaataa tttagtttgt
    11581 tacgtcttct tgtcaccctt ttaaataaat gatataattt aaaaaaacga agaggtgcac
    11641 tatgatttct acaaggtggt ttcagggtaa aagtaactta agcgatgttc tgagtatcag
    11701 gaaagaagta tttgctgaag aaaacagaga agaaaatatt acggattttt atgatgaatt
    11761 tgcttttaat gctgttttgt atgaagatga tgtacctgcc gtaacagcaa gactgtcatt
    11821 taaagaaggc aaatacttta ttgataagtt atgtataaaa aaacaattta caggattaag
    11881 atatgaggat ttgctggtaa ggctgcttgt tagaaaagca gttacaatag gagcagaaaa
    11941 aacttatatc gaagcagatg aaagtataga gaagatatta gaaaatatag gattcacaaa
    12001 agtaccgaat aataaaacat tgatggttaa agaaggggat gtgggcggac attgctgtgg
    12061 tgattgtcca gaaaaataat caataaatga tccaaaccgg gtatcattat aataatagaa
    12121 atatcttggt gaatacctac ccaaaaaaat atagacaaag ataacaggtt gttatataat
    12181 tactgaagta tagggtacac cttcttaagc gatagccttt gagaaaggat gttccatact
    12241 taggactaac caaaagtcac aattttagac tcattggtca agtggttaag acgccaccct
    12301 ctcacggtgg aaacaggggt tcgactcccc tatgggttat taaagaaaga tgacaagggg
    12361 acgggggtaa tgtcataatg acaacaactc cgtcccttta aaaaaatgtc ccgtacaagg
    12421 aggtttgaga tggattataa aaaattagca gaattgttat ttccaaatat aacaaagact
    12481 attgaatatt atgaaaatga agtatatcct aagagaaaat tgcctgaagg agccaaagta
    12541 acaagattag caccaagtcc aacaggtttt attcatttgg gtaatcttta cggagcattt
    12601 gtggatgaac gtttggcaca tcaaagcggt ggtgtttttt tgcttagaat cgaagatacc
    12661 gatgaaaaac gaaaagttga aggctctgtt gaaacaataa taacatccct tgaatacttt
    12721 gacttgaaat ttgatgaggg tgcgggaatt gaaggggaaa caggagatta cggaccatat
    12781 tttcaaagca acagagctga gatatatcag tgctgtgcta aacacctggt tgaaatgggg
    12841 agagcttacc cttgtttttg cactgaagag gaattggaag aattaaggaa agagcaagca
    12901 aaagaaaatg taaatacagg atattacggt aagtgggcga gagacagaaa tttatcatta
    12961 gaagaagttc agcagcattt aaacaacaat gaagaatttg ttattagatt tcgctctgaa
    13021 ggaacagaag aagggaaatt tgaaatagtc gatgccataa gaggacgttt aataatgcct
    13081 gaaaattatc aggatattgt aattctgaaa acgaacggca tacctacata tcattttgct
    13141 cacgtggtag atgaccattt aatgggcata actcatgttg taaggggaga agaatggctg
    13201 tccacacttc ctatccatta tgaaatattt aaaacaatag gttgggaaac acctgtatat
    13261 tgccatacgg cgcatttaat gaaaatggat aatggagcca aaagaaagct ttcaaagagg
    13321 aaggaccctg aattagggct gggatattac agggaattgg gttatcatcc gaaagctgta
    13381 agagaatacc ttcttaccat tttaaattca aactttgaag agtggagaat gaaaaatccc
    13441 gaaaacccga tagatgattt tcaattcact ttgggaaaaa tgagcaattc aggagcatta
    13501 tttgatttag acaagttaaa taacatttgc aaggatgttt tattaaaaat tcctgcagct
    13561 gaaatatatg aatttttgct taactgggca aatgagtaca aaaaagaagc ctatgaactt
    13621 ttgaaatcaa ataaagatga agttataaag ctcttgtctg tcggaagaga tggaaacaat
    13681 cctcgtaagg atttgatgta ttgtgagcaa atactggatt tcataagtta ttactttgat
    13741 gaatatttta aaattgttga tagttatcct gaaaatatag atagtgaaga agctaaaaaa
    13801 cttcttaaag cttacttaag tacctacgac cacggtgacg atcaaagcca gtggtttgaa
    13861 aagataagag taattgcatc tgaaaacgga tatgctgcaa agccaaagga ttacaagaaa
    13921 aacccggata tgtacaaagg acatgtggga gatgtaagta ccgctataag aattgcaatc
    13981 ataggccgcg catcatcacc ggacttatgg gaattgcagc agataatggg agaagaaaag
    14041 gttagaagaa gaattgaaaa tgcaataaac agttaaggga cagtggggac gtacactact
    14101 gtcccgctgt tatagtttag ggtttgagtt gaaatactca aacccttttg attttgttat
    14161 ttgattgtag tgaatgggac agttagtgta cgtccccagc gtcaccaaat tattctgtat
    14221 aaattggctg ttataaaaca taccaaaaat gcgataccgg tagggattat aaatgctgcc
    14281 aaagtccatt tcaaacttcc ggtttctttt tttattgtca ataaggttgt ggagcagggc
    14341 cagtgaagca gggaaaacaa cataacattt atggcggtta attgagtcca accatttgat
    14401 atcaatattt gtcttagaga atcaatactt tcaaaatctg tcaaagtacc tgtcgacata
    14461 tatgccatca atagaaccgg aattactatt tcatttgcag gaaaaccggc tataaaagca
    14521 ataagtatga atccgtcaag tcctattgct cttcctaagg gttcaaaaaa attagccata
    14581 tgaatcagaa tgcttaactc acccacataa atatttgcta aaagccatat aattgctcct
    14641 gcaggtgctg caacaactgc agctctagac aacacaaaaa ttgttctgtc gataagtgat
    14701 gtataaatta ttctgcctac ctggggagcc ctgtagggag gcagctccag agtaaaggtt
    14761 gagggaatcc cttttaataa agtattagac agtaaatatg acactgacaa agttgcaaat
    14821 acacctatta gaactatcaa ggttattgat aaagcaggta taatgctgtc aacaaatgag
    14881 ccggtaaaag caaaaaatgc tgctgacaat actataatca gcgggaatct gccgttgcag
    14941 ggaacaagat tattggtaat gacggcaatt aatctttccc ttggcgagtc aataattctg
    15001 catcctataa ctcctgctgc attgcatccg aaccccatgc acattgaaag gcacatttta
    15061 ccgtgagcgc ctgctctttt aaacagatgg tccaaattaa atgctaccct cggcaaataa
    15121 cccaagtctt caaataaagt gaacaagggg aaaaatattg ccataggagg aagcattaca
    15181 gaaataaccc atgcaaggct tttatacata ccatcgataa aaacacctct tataaaatca
    15241 tttacaccta agattagaag aattttgtct aatttatatt gtaaaccgaa caataaatca
    15301 gctaaaattt gtgaagggat atttgctcct tgaatagtaa tccagaaaat cagccccaat
    15361 agagcaagca taatgggaat tccgaatttt tttgaagtta caatatcatc aatttttttg
    15421 tctctgttaa gtttgttaac atcctccttt acatattttt gttttaattt attggcataa
    15481 ttatagttgt cttctgaaat tttttctctt atactttcat cctgattaaa atacaatgat
    15541 ttttcagttt cctcttcaat gatttgtgat gttttttcaa ttaaatcttt aagctccttc
    15601 ataccctgac cattccttgc ggaagttagt acaatcggta tttttaattc tttttcaaga
    15661 ccttctttgt caattttaat gttttttttc tttgcttcat ctattaaatt aatgcaaagt
    15721 attattttat tcgtaagttc cgacacctga aaaaccaagt ttaaatttct ttcaagacac
    15781 gtggcatcag caactaccac aaccgtatca taattgccgt aacatagaaa atctcttgta
    15841 acttcttcat cttttgaagc ggtgaacaat gaataagagc cgggcaggtc aattagtata
    15901 tattcattat ttttgtattt aaactggcct cttgcagaaa caacagtctt acccggccag
    15961 tttcccgtat gttgattaag tcccgttaaa taattaaaaa cagtgctttt gcctgtgttt
    16021 gggtttcctg ccaaggctac tacatactgt ttatcatttt tttctatttc ataaatatct
    16081 cttaatgatt ccaattttac ggattgtgct gtaagtccca tgctgacacc ccctatagaa
    16141 cttcaattaa acttgattct tcatttctaa gggcaatcat tgctcctctt attctgtaaa
    16201 cggttaaatt gttttttgga ccttttctta atacttcaac atctgcacct tttgtaagtc
    16261 ccaaagcaag aaatctttcc tttaaataat catgagcaga gttttgttta acctttactg
    16321 tttttccaac accaacatca gctaatttca tatttaaact cctgtaatat ttaaaatata
    16381 actgcataat attttttaga acatgttagc caaagctaac attctataaa ttataatatg
    16441 acattgctcg aattttgaca caattaggag gcattatgaa agaagagttt tatacactaa
    16501 aaggttataa aataaaaaat gaagaaatcc tctcttcctc aatggaggat tatatagaaa
    16561 tgatatgcag attgtctgaa aaatcagatg aagtcagagt aagcgagctg tcttcagcct
    16621 tgaatgtgca gcctccgtca accacaaaaa tgataagaag gctggcaaag gaaaattatg
    16681 taacctatga aaggtacgga tttattaaac ttactgaaaa aggaagggaa gtaggcagca
    16741 aattattgga aaggcaccgg ataatttttg aatttttaga atgcatagga gtaaaggaaa
    16801 atattttgga gcaaaccgaa ataattgaac atgcggtaaa cgatgaggtt ctaaataaaa
    16861 tagatgaatt gacacaattc ttaaaaaaat gtgataaata taaataagat gctgctataa
    16921 aaaatgctca aatcgtcatt ctgaacgcag tgaagaatct cataacttaa gatccttcgc
    16981 tgcctatcag gatgacaaaa ttttgttagg gcatcttatt ttttattgtt atcaacgtct
    17041 ttttttcgct cttgcacttc aaattcaccc ggaatattta cattcccggg attggcagat
    17101 cttctatatc tcattttcaa tttgtttgtt aacttcttaa acataatttc accaccttta
    17161 ttatagtttt acttaatttt agaaatttat tactgtttat aaaactttga aactaaggtt
    17221 ttttaattat tatcattaac ttattaatca gttttgaata agatgatgta aggagtcaca
    17281 gtatgacgga ggtagatatg gtatatattt gtgaactgag aaatgtctct aaaagatttc
    17341 atacaataaa taacgagaca aaggcaattg atgatatttc atttaaggtt aaaaaagggg
    17401 agatagtttc tatagtggga ccttccggct ccggaaagtc aacggtttta tcgcttatag
    17461 ccggattgga gaagccgtct gagggggaag ttattgttaa taccgacagt attggtttta
    17521 tgtttcaaaa agaccagtta tatgaatgga gaaatgtata taaaaactgt cttcttggtt
    17581 tagaagtaaa aaaaatggat aatgaagaaa acaagataaa agtaaaaaga atgcttgaaa
    17641 aatatgggtt gaccaatttt aagaaccatt accccagcca attgtccgga ggaatgagac
    17701 agagggctgc tctggtgcgc actttggcaa ttgagccgga gctgctttta ttggatgaac
    17761 ccttttcggc attggattac cagacaagac tttcaatagg tgacgaagtg gggacaattt
    17821 taagaaatga aaaaataagc accatacttg taactcacga cattcctgaa gcagtaagca
    17881 tttcggacag agttatagtc ttcactgaaa ggccggctaa aattaaaaag gaatatgaaa
    17941 taaaatttga ggagtgtcca ggtgaaagaa cacctatgaa atgcagaaat accttggctt
    18001 ttggtgaata tttcagaaat atatggaggg atttagatgt gggaaaagaa gtttgagccg
    18061 gttgcaaaca tttcagaaga gcacaaagaa tatttgaagt taataaatag ggaaaaaaac
    18121 tttattagga ttgcacaaat cttgattttt gtgtttggaa taatattttg ggaagtagca
    18181 gccaggaaaa atttaataga tacatttcta acaagcagtc cgggagcaat tcttggtctg
    18241 attatgaagc ttgttgatga cggcagcctg tttaaacatt tattcatttc gacaatggaa
    18301 acaataggcg gattttttgc aggtactgtt ggcggcttat taattgcaat aattttgtgg
    18361 tggtttagaa aattggctaa ggttatggac ccttacattg ttattttaaa cagccttcct
    18421 aaaacggcct tagctcccat aataatcttg tgggtaggaa tgggatattc aggaataata
    18481 gctacggctg catccatttc aatcgttgtt accactatga cattgtacac aggattttca
    18541 aatgttgaaa aggataaaat tattctctta aaaacaatgg gagcaaacaa gtttcaaatt
    18601 ttaaaaaaag taattatacc tgctaacatt ccaaatattt taagcgttat aaaagtaaat
    18661 ataggattat catggattgg ggtcattgtt ggagaattct tggtttcaaa agcgggaata
    18721 ggatatttga tattgtatgg aagccaaaca tttaaattag accttgtaat gatgggagta
    18781 tttgtattag gagtgattgc agcaattatg tatgtttttt cttcctggct tgagaaaaaa
    18841 ataattaaat actaagtatg accttcacat tatccttctg aataagcagc ctgtaattct
    18901 gaacgcaatg aagaatatga taaccttact gtctcaacga gacagttttt tgtcatatgt
    18961 atggggacaa accttaacaa agtcaagact ttgaaggtaa agttttgaaa gaatgtcccc
    19021 tgctttttca tcttgaaaca taattaaaat tatgttacac taatacagaa aattaaagga
    19081 ggtacatacc tatggcagca gtttcttcaa tattttatcc gctaatattt ttagttttaa
    19141 tatattattt atttaaaaac accaaaaaca gaatgcaata taaaaaagaa aacatggaag
    19201 ctttgctttt cttggatgaa gacgataaaa gggtacactt gatgagcaga ttacttatgg
    19261 cactaatgat tttgatatcg ggcataatga ttcgtggatt gatagaaaca aagacatatg
    19321 ctgcagagca agtattgata atgggagtac ttcctatact gataattaca ctgtatatac
    19381 ctttgtccaa gaaaacaatg atttcaacgt tgggaataca caaaagagga aacctcataa
    19441 gatgggaaga tattaaaggt ataaattacc ctaaacctga tgaaaaaggc aaagttaagg
    19501 ttaacataat ttatagcgta atgaataagg attcaaatat ccatcttaca tttttaaaga
    19561 atgaccctca attggataaa tttaaagaac atgcaaaaac ctacaggaat actaaaaaga
    19621 aggataagaa aagtggtaag taaatttata gacagtcatg cacatcttga tgataaaata
    19681 tttgaaacag acagggataa tcttattagc tcattatatg acaatgatgt tgaagctgta
    19741 ttaaatccgg gtgccgattt acaatcttca aaagaagctc tattaatggc aaataagtat
    19801 tcctttattt atgccgctgt tggatgtcat cctcacgata caaaatatat gagtgatgag
    19861 catatgaatg aattcataga gatggcaaaa aataaaaagg taataggaat aggcgaaata
    19921 ggtcttgatt actactatga caattcagac agggatacac agagaaaatg gttccgtgag
    19981 caaatacgct tagcaaaaga attaaatctt ccgtacattg ttcatgacag agatgctcat
    20041 gaagatattt tgagaattat gaaggaagaa cattatgacg gagcaagagg aatacttcac
    20101 tgttattccg gaagcgttga attgtcgaaa gagtttataa agcttggatt ttacatatct
    20161 ctggcgggac ctgtaacata taaaaacgca agggtaccca aattggtcgc aaaagaagtc
    20221 ccctttgata agcttttaat tgaaactgac tctccctatt taaccccggt gccatataga
    20281 ggaaaaagga atgagcctaa atatgttaaa tatgttgctg aagaaatagc tagaataaga
    20341 aatgtatcaa ttgatgaggt agcagaaaaa accaaggaga attttaaaag attatttaat
    20401 atagaggaag ggggacacac ctttaaatga aagatagtct ttgaaggtaa gcatttaaag
    20461 gaatgtcccc gtaataatta tgaagcttaa cagaaatttt tataatagag atgctcgtat
    20521 tgtaggagaa gacttaatag gaaaaatttt agtcagaaag tataacaata cggtaataaa
    20581 ggcaaggatt gttgaaacag aagcatacat agctgcaatt gataaggcaa gccacggtta
    20641 tggaggaaaa ataacacccc gtacgaaaat tatgtttgga cctcccggct atgcatatgt
    20701 gtattttatt tacggcatgt attattgttt aaactttgta acagaagaag aagggacctg
    20761 cagtgctgtt ttaataaggg cagtggaacc ggtaatcaat aaagaagaaa tgtgttttaa
    20821 cagatataag gaacatttgg atgatattac aaagtatcag aaaaaaaaca tttcaaacgg
    20881 tcccggaaaa ctttgcaatg ctttaaaaat aacaatagaa cataatggaa cggatttgac
    20941 aggcaatgaa atttatgttg aagatgacgg ctttaaagac tttaaggtag aaaagagcaa
    21001 aagaataaat attgattatg cagaggaagc gaaagatttt ctctggagat atacaattca
    21061 tcataaaaag gcttgacaaa aaatattttt acaaatataa tttgaatatc aaagtaatcg
    21121 gggtggttta atgagtaatc atgaaacagt aaatgaaata cttgtgaggc tttttgcaaa
    21181 catattgtat atcgaagaaa aatgcctgaa aataggggaa tttaaagatc taaccataac
    21241 agaaatgcat ttaattgaaa acataggata ttcaactgaa cgcaccatgt ctgatactgc
    21301 caaggctgta aaggttactt ccggtacatt aacaactgcc atagacaacc ttattaaaaa
    21361 aggatatgtg gaaagacgcc gctcatcaga agacagaaga gttgtaaaaa taagactgac
    21421 ggaaaagggt aaagcagcat acaaatctca tgaagacttc cataaagatt tggtaattag
    21481 tgcattacaa cagttaaaca gcgaagaaga agaagttttg ataagggtgc ttacaagcat
    21541 agaaaaattt ttcagtgaaa aatataaata tgcaggatgg tattgactat agaacaaaat
    21601 atatgactat gaaaagaggt taatatgggt ataagaattg taatcgacag tacttcggac
    21661 gtaacggatg aaataattaa aaagtataat ataaaaatgg ttcccctgac tgtcaatttt
    21721 gaaaatgaat catttttaga caaagttgaa ttgaattcaa aacaattttt tgaaaaactt
    21781 gaaactgcag aaaaactacc tactactacc ttagtgtcac cgggaacatt tatggaagta
    21841 ttcagtgata ttttattaga aggagacaag gtattggggc tttttcttgc ttccgaattt
    21901 tcaggaacat atgattccgc ctgtatggcc aagaatatga ttggcagcga tgatatttac
    21961 ataattgatt caagaagtgt ctgtctcggt gcatttgcac tgatattgga agcaatcgaa
    22021 ttagtacagc agaacaagga tatcgaagaa atagtgaaag aattagaggc actaagggaa
    22081 aaagttgttg ccgtagctgc acttgacacc ctaaagtatc ttgaaaaggg aggaagactc
    22141 tcaaagggac aagcggttgt aggcactcta ttaaacgtta agccgattgt cgaaattaag
    22201 gatggaaaaa ttagcgtaat tgaaaaaatt agaggtaaaa ataaagctgt aaaatggttt
    22261 gatgaatgga tagcaaagaa caactatgat ttatcaaaca aaacagtgct cctgtttcac
    22321 ggaaaagctt acgagcaatt aaagatgtta agagaatcaa ttgaagataa atataaaata
    22381 aaaaatataa tcgagcagga aataggagct gttatcggaa cccactcagg tccgggagta
    22441 ttaggaatcg gatttgtaaa caaataacaa taataaaagg ggaacttcct tttgaaaggt
    22501 gttccccttt attttttttg tcattctgaa cgcagtgaag aatctcatga gttccttcgc
    22561 ttaaacacag gatgccaaaa cgtatcagcg gatagcaaaa tgcaggtgac taaataatcg
    22621 tcagtacagt ttttccttca tcatttacat caacagtaac caatgaattt tccttaaata
    22681 caccttttaa atatgcttcc gagatttcgt cttcaacata cttttgtatt gcccgcctca
    22741 aaggtcttgc gccatacttt gtattatagc ctttttcaag tatatgttct ttcattttat
    22801 cggtaaccgt caaagtaatt ttcttttctt cagcttcttt aataacatcc ttaagcatta
    22861 aatcaacaat ctgacttaat tcttccttgg ttaattcgct aaatactatt atatcatcga
    22921 tacgattcaa gaattcaggc ctgaatgctt gtttcagagc atcctttacc ttggattcca
    22981 ttgcttcata ttctgtgttg ccaaaaccga ttcggttagc cttgatagat gttcctgcat
    23041 ttgaagtcat aattattacc gtattttcaa aatttacggt tcttccttga ttgtctgtaa
    23101 gccttccgtc ttccaatatc tgcagcaata tattaaatac atcttcatga gccttttcaa
    23161 tttcatccaa taaaattacc gaatatggtt ttcttcgcac tttctcggtt aagaatcctg
    23221 cctggtcaaa gccgacatat ccgggaggag ctcctataag tttggaaacc gtgtgttttt
    23281 ccatatattc ggacatatca agccttatca ttgcatcttc attctcaaac agttcataag
    23341 ccaaggttct tgcaagttcg gtcttaccca cacctgtagg acctacaaat ataaatgaag
    23401 atggtttttt ctttttctta aatcccgaac gatttcttct tatagccttt gcaaggcttg
    23461 taactgcagg atgctgacct acaactctcc tgtgaagtct ttcctctaaa tgtattagtt
    23521 tatcggcttc agcttcgctt attctctgaa cgggaatttt agtccaagct tcaataacat
    23581 acgcaatatc ttcttcagtt aaaagaatat tctcgtattc attggataac tcttttaatt
    23641 ttgattcaat tctgatttct tgtaccttaa gctctgcagc tctttcataa tcatcatttt
    23701 ctgcagcttc ttctttttcg tctcttattc tgctaagctc tgcttttaaa gcttcaagtt
    23761 caaccaatcc tttgttcttt aaatttgccc ttgaaccggc ttcatctata acatcaattg
    23821 ctttatcagg aaggtatctg tcggtaatat atctttcaga cattaaaact gcctgcctaa
    23881 taatttcatc ggttattttt acattgtgaa actgctcata ataatctttt attcctttta
    23941 taatttcgat ggaatcctct atggaaggtt catctactat tacgggctgg aatcttcttt
    24001 caagtgcagt atccttttca atatgcttcc tgtactcatc caatgttgtg gcaccgataa
    24061 tttgaacatc acccttggca agtgccggtt taagtatatt agcggcattc atagctccgc
    24121 cttcagcttc tcccgcacct acaatattat gaagttcatc gattacaata actatattgc
    24181 ctgctttttt ggcttcatca ataatagctt tcatgcgagc ttcaaattga cctcgaaact
    24241 gtgtgccagc cacaacggca gttaagtcaa gcaggtaaat ttcggtgttg aaaagtttta
    24301 cgggaacttt tttttcggca attcttaccg ctaatccttc agctatcgca gtcttgccta
    24361 cacccggctc accgattaaa acgggattgt ttttagagcg tctgtttaat atttgcacaa
    24421 ctctgtcaat ttctctctct ctgcctatta tcctgtctaa ttcgccaaat cgtgcctgct
    24481 ctgtcaaatt caatccataa gtatcaagat attttaattt ttttcgtttt actttttcct
    24541 ttattttagg acctgttccg gaacccggcc cggtatagtc tgcatcctca tcctccggaa
    24601 taaattcatt gcttgtagag ctggtgtttc ccgtaaaagc attattcatt aaattcatta
    24661 aagaattaat gccgtcactg ctttcagaca tatctccgga tagtttttct aaatccatat
    24721 ctcctattat ttcagtaagc tgtttgttta tattatcaaa gtcctcaggt ttcatgccgc
    24781 tttgtctcat tatctgatcc ataggtgcta taccctgctt tttagcgcaa ggaatgcaca
    24841 aacctgtagt ctgcatttta ccttcaacaa ttttattaac atatattact gctacattct
    24901 ttttacatat tgagcacaac atattcttca tctcctttcc tcgaggacat tcctttttac
    24961 ccgtcctcat taggagccgg gtataccgac agtattgatt ataatataat attcttaaaa
    25021 atgcattaaa aatttgtaga aaaaactatt tgcatgaatt gcttgaaatt ttgtccatat
    25081 ttacaagcta ctgccattat aaaaaatata atattatgct tgacatgtaa tgtcaacagt
    25141 attatacttg gagaaattaa atattttcat gggcatatta cagaggtcgc gatgatcaag
    25201 agtagaagct tttcttcgaa agggtgattc gccgaagggt taatccctgg gctcacattt
    25261 aatagatgtg atactgtttt tataggatgc atcccaatcc tataaaagag cgctgtaata
    25321 tgttgggcag aaggttcgat gttttttaag cgtctgagag cccttttgct cttatgtcgc
    25381 tttttttata cctcaatatt tgtacaagct ccggcgaggt acagacggat ggtaggtcat
    25441 agggagcgac atgaaattat tttatttata aaaaataatt taaaggagta agaagatgaa
    25501 tgatttagta agaggatgcg gagtagcatt ggtgaccccc tttgctgacg gagaaattga
    25561 ttatgaggtc ttaggaaaat tagttgagat gcatgtggaa aattccggct caataatagt
    25621 tgcaggaaca acttctgaag cagcaacaat gacagatgaa gaatatcaca cagtagttga
    25681 atttgtaatt gacagagtca ataaaagaat tcctgttatt gcaggagccg gaagcaattg
    25741 tacacaagct gcagtaaaaa aggcacagtt ttgtgaggag gcgggagcag acggcctatt
    25801 gatagtaact ccctattaca acaaaacaac tcaaagagga cttgtaaatc actataaaat
    25861 aattgcagaa agtacatcac tgccaattat tgtttacaat gtgccggcca gaacaggaat
    25921 gaatattgaa ccaaaaacag tcttagagct ttcaaaaatc aagaatatag taggaattaa
    25981 ggaagcaagc ggagacatca gccagattgc agaaataatc agaatatgtc cggatgactt
    26041 tctggtttat tcaggcaatg acgatatggt agtacccctt atgtcattag gaggacatgg
    26101 agttatttca gtatctgcaa atatacttcc tgcagaaatg aagaaaatgg ttgatgatta
    26161 cagaaacgga aatgtaaatg atgcaaagaa tgcccagctt gcactaaatg gaataaataa
    26221 agcattgttt attgaaacaa atcccattcc cgtaaaagaa gcaatgagta ttatgaactt
    26281 gtgcagctct gaggtaagag cacccttata tcagatgtcg gaagaaaaca gagcaagact
    26341 tctaagagaa ttgaatttat tcggaatagg cgttccttta gaaaccgcgt gaggtggagt
    26401 atgaatgttg taatttgcgg aataaacgga gctatgggaa gagttttgca agaagagata
    26461 aaaacatatg acaatattaa tctaatcggc tccctttcac caaggagcgg gagatttgga
    26521 caagatatag acggtatgcc tgatgtagta atagactttt caaacactgc aaatattgat
    26581 tttttaattg aatatgcaac aaaaaataaa tgtgcattat tgatttgcac aacaggcttt
    26641 agcgatgagg ataaagctaa aataaaagaa gcagggaaag caattcctgt actgttatct
    26701 tcaaacactt cattaggaat aaatgttttc agaaaaatat taaaaagtat ctctgcagag
    26761 ctaagtactt ttgatattaa cattgttgag aaacaccata ccaaaaaaat agatgctccc
    26821 agcggaacag caaaaacatt gtcggaagac attaaaaatg cgacaggaag ggagcatgtg
    26881 gaaatcagtg ccataagggc aggaaccatc cccggagagc atacggttat attttccgga
    26941 gtagatgaaa ttttagaaat aaagcatacc gctttttcaa aaaaaatatt tgctcgcgga
    27001 gctataaact tggctatgaa actttcatca aaaagccccg gttactacct tatggaagat
    27061 ttcttttaac aaaaataaag acagcggagg gaaaaatgaa caaaataaga ataggaattg
    27121 tgggatacgg taatatagcc agaggagtgg aagctgcggt aaatttaagt acagatatgg
    27181 aattagtggc tgtcttcacc agaagacagc ctgatacgat aaaaactcag actgacgtaa
    27241 aagtattaag tgttgatgag gttgaaaaat ataaagacat aatagatgtt atggtcttgt
    27301 gtttaggctc ggcaacagac ctacccgtac aggcagctga atatgcaaaa atgtttaata
    27361 ctgttgacag ctttgataat cataaaaaaa ttccggaata cttaagaaaa gttgatgaag
    27421 gtgctctttc ttccggcaaa ataagcataa tttcaatcgg ctgggacccg ggactgtttt
    27481 caattatgag gatgctgtca gagtcagtcc tgccggaagg cagcagctat accttttggg
    27541 gaagaggagt aagccaagga cactctgatg ctattcgcag gattccgttt gtaaaacagg
    27601 caattcaata cacaatacct gtggaggatg cagtaaattg cgtaagacgg ggagataacc
    27661 ccgaacttac tgcaagagag aaacacataa gacaatgcta tgtggtttgt gatgaatgtg
    27721 cagataaaaa taaaattgaa gaagatataa aaaacatgcc ggattacttt gcagaatata
    27781 atacagaagt taatttcata agcgaagaag aatttaagaa aaatcattcg aggatgtccc
    27841 atggaggact tgtaataaga aacggtatga cgggaagtga gaaaaaccaa catataatgg
    27901 aattctcact taaattagac agcaatcccg agtttaccgg aagcgtgatt ctatcgtatg
    27961 caagagctgc ttacagaatg aacaaagaag gaattaaggg agcaaaaacc atttttgatg
    28021 ttcctctttc atatatacat cctgaaggca gagaaaaatt aataagaaac tttttataat
    28081 tgtttacgaa ataaatacat tcgtattaaa aacgttgaca tttatatttc cttatgatat
    28141 tgtaaaaaag ttataaatta atttggagga agaaatgtta aaggttgcta aatttggagg
    28201 tacttcatta gctgactcta agcagtttaa taaagtatat gacataatca aaaacaatga
    28261 tgaaagaaaa tatataatag tatcagcccc cggcaaaaga ttcaaggatg acaacaaaat
    28321 taccgactta ttgtatctta cgtatgcaca tataaagtac tcggtaccct atgctcctat
    28381 tttaaaaatt atagaagaca gattcaggca gattaaggaa gagctgaact tgaaaacgga
    28441 tttagactat gagtttaaaa ttataaaaga aaacttggac aataaatgtg aagaagacta
    28501 tatcgtaagc aggggcgaat atttatgtgc cttgttgatg gctgattact tggattgtga
    28561 ttttattgat gcaaaggatg ttattttttt taactacgac ggtacggtaa atgaggaaaa
    28621 aactcatgaa aaactgacag aaatactggg caaaagcaat aaggcaatta taccgggctt
    28681 ttacggctca tatccggacg gaagcataaa gactttttca agaggaggct ccgatattac
    28741 cggctcgatt gtttcgagtg ttatgggagc agatatatat gaaaactgga cagacgtatc
    28801 aggattttta gttgcagacc ctcgaattgt aaacaatcct aagcaaatta agaaaataac
    28861 atatcaggaa ttaagggaat tgtcatatat gggagcatca gttttacatg aggaagcagt
    28921 ttttccagtg agacaagcag gtattccaat aaatattaaa aataccaacg accctgaaaa
    28981 tcccggaact ttaataatca gagatgacca agcaggagaa gattatgcaa acggacatat
    29041 tattacgggg attgcaggaa gaaaagattt ttcatttttc tatattcata aggagcacat
    29101 ggcacatgag gtgggtattg taaaaaaagc cctggaagtg tttgaagaaa ggggcataag
    29161 cattgaccat attccttcag gcatcgacag ctttacaatt gttcttcctt cagaatccgt
    29221 tgaaaaaatc agtcatcaaa ttgttgaaga acttaagaaa aaatgcaaaa cagataatgt
    29281 acaagttttt agaaacctca gtcttataac cacagtcgga atcaaaatgg cgtatcgacc
    29341 cggtatatca gctaaattgt ttacagcctt aggaaacaga aatattaata ttcgtatgat
    29401 aaaccagggc tcaagcgaaa taaacattat tgtggcagtg gaagacaaag attttgaaga
    29461 agctataagg gcaatatatg atgcattcat aaaataaata aggggacaca tagggttatt
    29521 cctctataag ggtgtgtccc ctttaccatt agctgtgttt taataatttt atcaaataat
    29581 tattttatat atccgccaaa catagttttt atttcattta ctgtaatgta tgcttcagga
    29641 ttatgctctt ttattaattt tgtagcaaca ggaattttct ttcttttcaa ctgaataaat
    29701 aaaagatttt taacactatc atttttacct tttccttcga gtatcgtaac accaaatcct
    29761 tgttccctca aaatatccgc aagaacctct ccctcttttt ggtctacaac gacctgtatt
    29821 gaagataatc ccacggcaat ttttgattct atagtaactc caaagaaatt gcccaaagaa
    29881 aatgctatcg cgtaaataaa aacctttata ggatcttcag taatattgtt taaaacagag
    29941 cttacaacaa taagccaaat cattatttct ataaagccta aaagggcacc cttgattttt
    30001 tctcctttat taataaagac tattctcact gttgaaattg aaacttcaat aatttttgca
    30061 aaaaagatta taaagtacag caacggaccc tctaaattca taaaaaaatc catttgttat
    30121 gtccacctcc aaatttattc tactcatttt atcagtaata tgcaatatac gcaaatatta
    30181 ttttattaaa gacagctgat tatttttgtt ataataatac gcagcaatag ggttacataa
    30241 ttttatttat gtaaatcttt ttgtcattct gaacggatgt gaagaaccta tactcttgtc
    30301 attctgagag cttccgctcg aagaatctcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30421 nnnnnnnnng gatgtgaaga acctatactc ttgtcattct gagagcttcc gctcgaagaa
    30481 tctctactct tgtcattctg aacgcaagtg aagaatctca tcttttaaac caatagtaaa
    30541 atatcgtctg ttaattttca ttaaaataat gtataataaa ttttacttat ataaaaagga
    30601 aaataaacat gagattaaga tatgttccgg gacagtatca atatttaaaa aattgtgaaa
    30661 aagtaattac tgagcccgaa aaacaaggat ttaaactgaa taaagtattt aacaacaaca
    30721 atccgataca tgtggaatta ggttgcggaa agggaaaatt tctaagagaa aatgcaatta
    30781 ataaccccca tataaactat ttcggatttg agaaaagtgt caaggttgca tacagatgtg
    30841 ctaaatcagc ctttgaaaat gacccgccga attattacat cgtttattca aacggagata
    30901 tattaaagga agtatttgat gcaggttcaa tcgacagaat atatctgaac ttttcagacc
    30961 cttggcctaa accttctcat tacaaaagac gattgactca caaaaacttt ttggatgtct
    31021 ataaaattgt tctttccaaa aatggtgaaa ttcatatgaa aacagacaac aatgacctat
    31081 ttgaatactc ccttgaacaa tttgaaaagg acaactggga attcaaaatg gtaacaagag
    31141 atttgcacaa cagcatccat aaggaagaca atattatgac cgagtatgaa gaacgatttg
    31201 taaataaagg tataaaaata aataaattga tagtgagaag gaaaaatggt taacataaaa
    31261 tatattatgt tctacttatt aataataaat ctaatctcct ttgccttatt ctttgctgac
    31321 aaacaaaaat caaaaagaga caaatggaga attaaagaaa gcactcttca tttggtaagt
    31381 tttatgggtg gaacacttgg aagcattgca gcaatgattt tgtttcatca caaaaccaag
    31441 aagcctaaat ttgttataat tacaattatt gcattgcttt ttaatgtggt attggtatat
    31501 gaactcttaa taagataaaa gcagtatttc aattattata tgataaaaat aatgactgag
    31561 cactcagctc agtcatttta tcttattatg gtacttatat ttcaaaacca ccgtcaactt
    31621 taataatctg tccgtcaata aatgatgaat catcagaagc caagaaaagt gcagcattag
    31681 ctatatcttc aggaagtccc actcttttga caggactctg gtctatcata tgctgaacaa
    31741 cagaaggacc tccgatactg tcaaccattg cagtatgtat cagtcccggt gcaattccat
    31801 taacacgaat ttccggtccc aattcccatg ccattgattt tgtcagtcca ttcatagcat
    31861 gctttgatga aacataagca gcaaaaccat gatgagcacc aaatcctgca accgaagatg
    31921 tgttaattat aacgcccttt ccttttgctt tcattatagg cgcaaccaat tgagttaaat
    31981 ataatgctga tgtaacattt acattaaata tcttattcca ttcttcaaga cttacgtctg
    32041 ttataggtga catacttaga gcacctgcat tgttgaataa tatatcaaca gttccatatg
    32101 tatcaagagt aacatcaacc aattttttca catcttcgac ttttgatgtg tcgactagaa
    32161 catatatcgc ttcattgcct tcacctttaa tttggtctac cacagccttt gctctttctt
    32221 catttcttcc tgtaacaaca acctttgcgc cttcacgagc aaatagtact gcagtggcac
    32281 gccccattcc ggatgttgaa ccggtaacaa ttgcaactct gccgtctaat ttcttcaatt
    32341 gaacccctcc ttttattagt aatcattgtt atagttaatc ttataataag ataagtatta
    32401 agtcaatgtt aaaaaaataa cgataacctt ttcctcaaaa taattattaa cagggtttca
    32461 atatttggaa atagatggta taatagtatt atgatatcga tttcatagtg tttaatgata
    32521 ttagattaaa taaaaactca acaaaaattt atacaaatga aaaggagaac aactatggag
    32581 tatagaaatg ctaatataaa tgatatttac ggagtttcac agcttcaaaa aaaatatcat
    32641 atatatacaa taagtgaaga agataaacct gacggttttg taacaacctt gtttactgaa
    32701 gaacagttca aagaattaat agagaaagaa aatgggcttg ccatagcatg tgataatgac
    32761 aaagttatag catatgcaat ggcagcatca tgggattatt ggtcagcttg gccgcttttt
    32821 caatacatga taaatgactt gcctaacatc caatatttag gatacacact ttcaacagaa
    32881 aactcttacc aatatggtcc catatgcatt gataagcaat atcgaggcac cgaagtatta
    32941 ccaaaccttt tcgaattctc aagactccaa atgaaagaca aataccctgt attaataaca
    33001 ttcataaacc aaatcaacgg aagatcatac cgtgcccacg tggataaact gggacttgag
    33061 gtaattaaaa catttgattt taacaacaat cattattatg agctagggta tgatatgaga
    33121 aagaaaacac caggagcgaa tttgtataaa taaaaatata gcaaaatagt taatctaata
    33181 aaaaaaatta ttgtgtgtac tgattatagg acaaccaatg tgatatgtta aatttataaa
    33241 tatattataa ttcaaattac gtcaggctgt acctaactca tatggataaa ttggaactta
    33301 agacaattaa gtattttgat tttaatagca atcaatatta tgagttagtg gtatgataac
    33361 aagaaaatca aatataaaga aaagaagtag tatgataata aaacgtaagt attctaattt
    33421 agttctatta ttaataaaaa ataagatata aatttacatt ctaaatttag ttctattacc
    33481 atacacttat tgttatttaa tatatagcat aaagcagtaa aaaggtaaat gtaaaaaaat
    33541 gtaaatttat ttaaaataat tattgacaaa acgttttact tattatatga ttgttttaaa
    33601 cataagatat aatggtaaag ggggtgaacg gatgagaata tctgcagtgt tttcattgga
    33661 acaaaatagt atcgataagg acaagaacag aatatttatc tctattttaa aaaacatctt
    33721 ggaaagctat gatgtaaatg tatataagaa attctttgaa agcggaccag taagaaagtc
    33781 atattgtttt gcattatata tgaaggatgc agaatttacc agagacaaaa taataatacc
    33841 atataaaaag atagttttga aactatcaac agccgattta gaagatgcta ttcatatata
    33901 taatgctttt ttgcagaata taagaagaac attcaagatc agggaaaatg aattaaaact
    33961 tgataagatt agcattgaga gggaaaagaa aatttatgat gataatgtta tcttcatatc
    34021 tttatcgcct atattaatca gggaacatga aggtgataat aaaaagactt ggtatcatac
    34081 tctcaatgat gaaaaaggaa ggatgttatt tttgaacaat ttaaaaatcc aactgttaga
    34141 agagtttggt cgagaccgta ttcttgatat tgaagaagta aaagtcaatg ttttatctaa
    34201 taaaaatgtt aaggtaaaaa attattctat tgaagtagcg ggaaatctat gtacatttga
    34261 aatgcatgca aagtcctaca tattagagca tgtttataaa gccggaattg gtagccaaaa
    34321 aagcactggt tttggtatgt tagatttgtt ataggaggtg atgctgtgag tgaaaaggtt
    34381 agactatatc tggatgattg gctgtttaac agcggtcttg tgggttttta taatatttta
    34441 aaaaaatctg aagatacgat aactgttgga gaaaattatt tagagtttaa tattgaagcg
    34501 ttaaataatt ttgatgagaa atattttgaa tattttattt ctgcttatga aaagaatact
    34561 tcctggtata agattgtttc ttatgaagat gtaattaatc attataaaca aaataatttt
    34621 gaagagttta acaaaaagga catcgataat ttaaataaat acattaatga tgtgaaaaaa
    34681 ttcattaaga gcaacagtta tcaagcagtt tttgagtttt taggtttgca aaaagatata
    34741 tcttctttag aaaagcaatt aactgcaatt aagttaaaag atggacaaga tacaagggat
    34801 gttttcccgg atattaagaa tacttttaat atattgggaa aaattattga attcataaat
    34861 aaaaataatg taaagaaata tgttaaagca aagaatgtta tatataccat aattaataat
    34921 ggatggaacg gtgtatgttt tttaaatcca caaacaaaag agaaggatat gtataaggat
    34981 tatgaaaatt attttgtaaa gcctgctttt gaatatttta acgaagacaa gtcaacgtat
    35041 aaatatgaat gcttttcatg cgatagtaaa atgaaaaata tgaaagacaa tcttggtttt
    35101 ttgaattcta tcgggtttga tgtaaataaa aagtcatctc atgtttggaa ttttaataat
    35161 gatgtggctg tttgtccttt atgtaaatta atatactcct gtgtgccggc cggattctct
    35221 tatgctattg acagcggtat atacgttaat gacaactttt ctatgcaaaa tgcaattgac
    35281 gttaacaata agattaagaa ggaaattcta aaaacaaatg aaaacaaaag ggccctgaca
    35341 tatagagcat tagttgagtc tataaaagag caatttagta tgagtgcaaa gtatgaatta
    35401 tctgatgttc aggtagtcag atatgtgaat gaaggataca gatttaatat tttaacaaga
    35461 aatattttgg aattggttta tgaatgtagg gaagaattaa atagtttaat atcttgttgg
    35521 tataaagaaa tcaaaacgta ttttaatatt tatgatatgg ttctggacaa tttgtttaac
    35581 agtcaaaatt tatatttgct gatacacaaa atgcttctgt ataaattatc ggatttcaat
    35641 aattgttatt ataattggat gcaaacagtt aacgtaatgg aaataaattt taagtttatg
    35701 aggaggttag gatatatgga aagttgcaaa aacgatattg taactatcag taacaagagc
    35761 ggatattttc tgcgggaagc ttataaacaa aaggacccca acacttcgaa attaagcgga
    35821 atatcttatc gtttattgaa tgcattaaag gtaaatgaca aggacatgtt tatggataca
    35881 atactaaact gctacttata tgtaaaaaag tcagtgccgc cagtattgtt ggaaat
//
LOCUS       DAOSHP010000011        35027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_967_length_35027_cov_9.422910, whole genome shotgun sequence.
ACCESSION   DAOSHP010000011 DAOSHP010000000
VERSION     DAOSHP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 35027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35027
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_967_length_35027_cov_9.422910"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..145
                     /locus_tag="PLB33_02215"
     tRNA            73..145
                     /locus_tag="PLB33_02215"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Phe,seq:gaa)
     gene            complement(241..1233)
                     /locus_tag="PLB33_02220"
     CDS             complement(241..1233)
                     /locus_tag="PLB33_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOG62366.1"
                     /translation="MFINEYEVNNITDNPYLNEIYKEAVFIDIETTGLSSIFSDIISI
                     TFLLYKDDKYRIYQIFCQYKQDQPDALKYLNELIKLKKYMVTFNGNSFDIPFLAEKAK
                     QLNITLVFDSFNKIDLYNCIKKFRYKINTVDLKLKTVEKYFSIDRNDTLDGKDVITLY
                     EAYRLEQRKEFSYLILKHNYEDVYNLPFVMNNIFSLYDFVIYTKNLIITINNEDITIK
                     KNSIKCKFNILSLIAKDYINHSINYDMVLSVKPQTLKIDIPLNIYKDNNIREFYYLDN
                     DEYNIDNYTSIKGLKRNLIPIKLNNKMYYDNINRIVKEIVEEGFGCSEKQVPSF"
     gene            1300..3615
                     /locus_tag="PLB33_02225"
     CDS             1300..3615
                     /locus_tag="PLB33_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOG62367.1"
                     /translation="MYIIISIFTAYAFGIISYELNLYGYYFTALLAILIYNSLINKKY
                     MYNAVIIAFVILSYINCNYNSSSVLSQYKNESIVVEAKVKKQKKSGSESYYASVIGIN
                     NTPILNEENIILYTGKYKNFQENSIIKARVNVAGSQLNKNKMLFNYENYLRSKKINAT
                     VFADEDAYVIDENYSFLNNISSKFRKYTEKTFYYNLNKKNAEIILSIILGDVNYLEEE
                     LYDNIKVMGLAHIFAVSGTHIVLMYIFLLNVFKFVLSRRFSWLLSWCIIWFYGFLIGF
                     PLSIMRALVMFTLLFGSEVLYRKYNSLNSIGLAALVLTIYNPFWIFDAGFLLSFSAAL
                     SFIIYGKYIKTKTTVLRTVYMYLFLQIFTLPVIVYYFNFVPVMGVLYNLLLLPVFTFI
                     MIYGFILLILNSFAHIILIIPFNIYDYILHSLRYIIDISDKSAFNGLIMPTLSLCQTI
                     FFYITLFYMIYIHNNKKCNCKKIGIFAIVSLYSITYIAFPMMDDSLYLNIADVGQGLF
                     TTIKYKGLNMICDCGSTTNKQMGEYIAVPYLTKRGIAQVDGIFISHWDEDHYSGLNDI
                     LNSHIKVNNIFSSNSNENYTNVQKLNAGCQMKYDDNLSMEILWPYKDLSLNNTNNSSL
                     VISLNYKGRRILLPGDIEEEAEYAIYNGLNAYDIVLVPHHGSNTSSTVQFVDAVRPKI
                     AVLSYGRNFYGIPHEEVISRYRNTGSKIFSTFEDGEINIVLKDNNLYYNTYMNEKSDN
                     YYKLYLIGIIYKLSIFCFLLFWMLKGESYEL"
     gene            3605..4645
                     /gene="holA"
                     /locus_tag="PLB33_02230"
     CDS             3605..4645
                     /gene="holA"
                     /locus_tag="PLB33_02230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOG62368.1"
                     /translation="MSFNLIKNMADKNKLPNCLLLYGNEEYFIDYSIKYIKYKYIDKN
                     YEDMNYAEFEKPASLKDYFEFAYTFPFMSEKKLCVIKEAGFFTSTGSLDKKEEDRLLN
                     FIDENETCISIFVIKDGKADSRKKIVKKLKDNKAVFEFNRLTEGELTKYITEEFKKNN
                     FKISMSDANYMANNTGYLEYESTVSLYHVNNEINKIMSYNKEKKNITTSDLDLLMVKS
                     IESNIFRLVDCICENDKKSSFEILNEMLLNNTPEQYIFHMITRQYRMLYQYVMLQKKG
                     YSYNEIMSKMKIKNFVASKLAKQSKNLNMETIEYYMKRFLEIDSKIKTGEIDSRVGLE
                     LISNGIIGTGTN"
     gene            complement(4669..4932)
                     /gene="rpsT"
                     /locus_tag="PLB33_02235"
     CDS             complement(4669..4932)
                     /gene="rpsT"
                     /locus_tag="PLB33_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOG62369.1"
                     /translation="MANIKSAKKRILIINKKTQANKSRKSEIKTYIKKFETALSEGNY
                     EKAKEYLKICEKKLYQAAAKNTIHKNAAARKVSRLTKKFNKVA"
     gene            5149..6081
                     /gene="gpr"
                     /locus_tag="PLB33_02240"
     CDS             5149..6081
                     /gene="gpr"
                     /locus_tag="PLB33_02240"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOG62370.1"
                     /translation="MYRTDLAYEANESLAKKIEGITLNTHSYSHGEVVELEIKDKEAE
                     KAVGKKMGKYITYETKSLKSLSEKSRNEVIDILSKAIRDISGLGREKILVVGLGNRNI
                     TADSLGPKTLDKIKVTAQYFKAYNKDFDEDFNEVSILEPGVLGTTGIETINTIVGVVE
                     KIKPSLLIIIDALASRKMRRLCAVVQITDAGIEPGSGIGNMQGSLNKETIGTKVIALG
                     IPTVVDTATIVNDTIELMEKKLSNKTDDVGKIMGILSDLEYNEKHAFIKEILSPMYGE
                     SIVTPSYVDEIIDSLSSLLAESINRAVHPGYVNS"
     gene            6176..7324
                     /locus_tag="PLB33_02245"
     CDS             6176..7324
                     /locus_tag="PLB33_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOG62371.1"
                     /translation="MKKICGVDIMKRRRKNKDGRSFYMVMSIFCLTALFLGYSFVYNL
                     SEERKTGKEVTTIFINYNTDKSKDGNLSTFDKVMSYFNLFLEKTFKGKKSNDVELIEN
                     IDTQSYEKDIVRTIASEDTKKDLEIFNESRYEPESDSEVYLTSKRKENFFKVISKTSR
                     SSAPRRKLFINAASVINNFNIVLFHTHGTEAFEESYETNYRSLDENLNILGIGAKISN
                     NLLNKGIKNTHLKDYNDYPDYNSSYANSNYAVRQVLSDSKKNILIDLHRDGAEENSNY
                     EAILSEVKTTEINGKKAATCTLVVGNKNSNASKLKENAGRLYEIAYEMYPGLMRKIIF
                     REGAYFNQYLSDYSFLIEVGCTLNTYEEIEYTADLLTEVLYEYINEIE"
     gene            7392..9197
                     /gene="lepA"
                     /locus_tag="PLB33_02250"
     CDS             7392..9197
                     /gene="lepA"
                     /locus_tag="PLB33_02250"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOG62372.1"
                     /translation="MDRKKYIRNFSIIAHIDHGKSTLADRLIENTGLLTHRQMQEQVL
                     DNMDLERERGITIKLQTISLLYKAKDGNNYTLNLIDTPGHVDFNYEVSRSLAACEGAV
                     LVVDAAQGIEAQTLANVYLALDQDLEIVPVINKIDLPSARPDEIKQEIEDVIGLDCSD
                     APLISAKDNINIEQVLEAIVTKIPPPTGDEDAPLKALVFDSYYDSYRGVVAYIRIKEG
                     QVKKGDKIKMMSTGKTFDVTEVGIISPTQKPVPCLYSGDVGYLCASMKEVKSCQVGDT
                     ITNNANPAKEPLPGYKKVTSMVFCGIYPAEGEEYNSVRDALEKLQVNDASLIFEPETS
                     VALGFGFRCGFLGLLHMEIIQERLEREFDLNIIATTPSVIYKVYKTDGEMMVLQNPTN
                     MPPVQEIEYMEEPIVAASIMSPTEYVGAIMELCQNRRGTFVNMEYMEAKRVILHYKLP
                     LNEVIYDFFDSLKSKTRGYASLDYELDGYVRSELVKMDILINSELVDAFSIIVHKDKA
                     YERGRAIVERLRDVIPRHLFAVPLQAAIGNKIIARETVKALRKDVLAKCYGGDITRKK
                     KLLEKQKEGKKRMRQIGMVEVPQEAFLAVLKFDED"
     gene            complement(9338..9817)
                     /locus_tag="PLB33_02255"
     CDS             complement(9338..9817)
                     /locus_tag="PLB33_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62373.1"
                     /translation="MRVTIISESGDWKEMLYTKDIVTYEIKNKEILDFFVKLGDAYAN
                     NDTLDDMFECLYNLINYGYLYTDENEDDNYYDDFDYDEDYYDFDDEEQNYFSQDGFKL
                     IKTEVEYYVDNELVSKDEYDDAWDDRLGYLGSSNWEEKGLTIINADTDEIIFLGKLS"
     gene            complement(10023..10748)
                     /locus_tag="PLB33_02260"
     CDS             complement(10023..10748)
                     /locus_tag="PLB33_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG62374.1"
                     /translation="MIIRYIYHSCFLIETMNSFLLFDYFNHKNDKTDFDFSKLLKDVF
                     SSPKSLYIFSSHSHQDHFNYNVLSWINKKPSTYYILSSDIKIYNKIDNLFIVRPRDEL
                     TINEIKLNIFGSTDEGVSFMVNIDGLNLFHAGDLNWWKWYDDTPEEEKTMEDNFKSIM
                     ADIVKHGVKIDIAFFPVDRRLENNYLCGAQYFIDKLNPKLFIPMHFWDDYKVTSDFKN
                     AIAGHGTNIIEIKHPNEILYANI"
     gene            10939..12591
                     /locus_tag="PLB33_02265"
     CDS             10939..12591
                     /locus_tag="PLB33_02265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG62375.1"
                     /translation="MKIENIVLNNNIQRALDDMGFEESTQIQQEAIPLILEGKDVVGQ
                     SNTGTGKTAAYAIPILEKIDKELRVPQALILLPTRELAVQVANEVRKIGKYMEGIKAV
                     TVYGGADIREQINKLKGGAQIIVGTPGRIIDLIERHVIKLSELRIAVLDEADEMLKMG
                     FREDIELILSKIDHPVQSLLFSATIPDDIRKIIKRFLNSPVYVKVLREGITAKEVKQS
                     YFLVKHSDKVEALSRLIDTYTPKLTLVFCNTKKAVDELYNKLIDKGYNCDKIHGDIKQ
                     SQRLDTLNKFNNGLIDILIATDVAARGLDIKEVDLVINYEVPSKEDYYVHRIGRTGRA
                     GREGASFTLVSAKELRKIEDIEKYTKKSIRKRAIPTVDRVNEVKQDKFIRDIVNVIES
                     GDLGENRELAEKLMDKGYDAETVIAAMIKKLVKFDLSEERDLNEVIPERKKRGGKLEN
                     TERFHINLGKKQGIRPGDILGAVAGECDIPGYDIGEIDVLDNFSFFAASKEHKKRILD
                     RMNRSQIKGMDVVVELSKEKKSRFGRDKSSDDNSSRKRKKRK"
     gene            12807..15329
                     /locus_tag="PLB33_02270"
     CDS             12807..15329
                     /locus_tag="PLB33_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOG62376.1"
                     /translation="MNIIVKFITKSILEKKFRSFIIIFSVTISAALFFASSALAGTMS
                     SMYEALIRMQTGKADLLIYPGRESPSSTFKILPNQVKGADYIVGTFSVGGIYKLSEEE
                     YQIERKKTELLYIKGFNIDELEKLNPINYGQKATGREFSDNHIILSEIFAKKYDYNTG
                     DSIHIEIGGVDRVLTVWGISRPTGVFQHNPQSNSIAAVMPLDTLTSLYNRRGWVDTAY
                     IVVEEGYDILNVKEEISTLYSRYVVEEPFTAEVLNEYLQFLVVPLYLMTIMVLFISVF
                     IIYSTFKVITTERLPVIGTFRSIGATRKMTDIILIGESLLYGIVGGILGVLTGMGVLY
                     LIARIMASDPYNGQLDVSIKFGLVHMLTTFILALMVATVSSWIPISRASKIPIKELVL
                     NLIDREIIKKNKKVIILTVMFAISIILSFITQNIASMIFNLLSLLIIFALVIIYIPFI
                     TSFLLKFFQQVYGAVFGNEGILAVKNLKDNKNIYNNISLLAIGISVILMINIISYSVG
                     IEVLNAYKDWKFDIMLSLNGDRNTEQVLRSIDGVEGTYGAFESFQGIKVVDTDYSIGY
                     MQGIDIIKYRDYAAFRSEGNEDVDELFKKLDDGRNIMVTLMMKDSLGLKIGDKLTLDM
                     NSGEKTYNIIGFYDSIMQNGSNAIISQKYFKTDMEQPYYNRFFVKTSKEPELVLSEIK
                     EKFMRQGVWGNSISNMEKTNYEQNSQFMIILQAFSLLAMLIGIFGVFNNYMISFLERR
                     RYIAIKRSIGLSKKQTLKMIFVEALTGGCIGATVGIIGAMFMLWIVPELMQTIGVPLA
                     IHYKSDLFAASLFTGIIISVIASVSPALKTTKLDIIESIKYE"
     gene            15339..16022
                     /locus_tag="PLB33_02275"
     CDS             15339..16022
                     /locus_tag="PLB33_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62377.1"
                     /translation="MEKIIEVENLNKTYMLGNVPVEILQEINFTINRGEFITIMGPSG
                     SGKSTLLYLLGGLDKPTSGKILINNKDISALSDEEQSTLRRRIIGFVFQFYNLVPNLT
                     VEDNVMLPVLLDGKKIKDYREKLDEILETVGLSDRINHTPRELSGGQQQRVAIARALI
                     NEPDIILADEPTGNLDSKSSEEVMKLLQRINKVKGKTILQVSHSLETAKYSDRIINVR
                     DGKVWCNEN"
     gene            16012..17034
                     /locus_tag="PLB33_02280"
     CDS             16012..17034
                     /locus_tag="PLB33_02280"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOG62378.1"
                     /translation="MRIKAILMLLVIIISVAGCKAEDPQTTVEEIDLSQEKKSIEAFG
                     VVKPEEFMDIIIDFNSVVKEVSVNEGQYLKLNEPILLLDLSQYEADISDTVSELNIAE
                     LEYQQISMGFEDLSAEVNKLKNNIKYTEGLHQNTIKELDINKKLFEEGVISEEKYNQS
                     KLKAEEAENNLENLKFELSNAEDAIYKKTQSEKAKYAVQSEYIAQIKNRLNILQNKLN
                     KPYIAGNQIVSMYDNAVVYDIQYQPGHITDVSKKAFSIANLDSLTIEAEVVEEFIKDV
                     REGLKVKIVPAADRTIECEGRVIYVSDIAYSKNGGTVVPIKISLDNNDSFLKPNYNVD
                     VYIEVQ"
     gene            17394..19319
                     /gene="thrS"
                     /locus_tag="PLB33_02285"
     CDS             17394..19319
                     /gene="thrS"
                     /locus_tag="PLB33_02285"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOG62379.1"
                     /translation="MIKIKFPDKSVREYEKGIKALDVAKSISEGLARNVVGAVYNGET
                     IGLQTALNEDGNIKFLKFEDKEGKDIFWHTSSHIMAHAIKRLWPEAKLAIGPAIENGF
                     YYDIDLEHRFVQEDFEKIEAEMKKIVKAGYELERFELPREEAIKFMEEAKEDYKVELI
                     NDLPEDAVISFYKHGDFVDLCAGPHLESTKKPKAFKLLSIAGAYWRGNENNKMLQRIY
                     GISFEKAKDLEDYLKMLEEAKRRDHRKLGKELNLFMMSEEGPGFPFFLPKGVVVKNEL
                     MKYWRKLHKKAGYVEIETPLILNQHLWEVSGHWYNYRENMYITSIDEQDFAIKPMNCP
                     GGILAYKNSMHSYKDFPMRVAEAGRVHRHELSGALHGLMRVRAFTQDDAHLYILPEQI
                     KDEIKGVIKLIDEIYKQFGFSYKLELSTRPEKFLGEIKDWDLAEASLKAALDELNLDY
                     VINEGDGAFYGPKIDFHLTDCIGRTWQCGTIQLDYQLPQRFEMEYVGADGLKHRPIMI
                     HRTAFGSIERFFGILIEQYAGAFPTWLAPVQVKILPISDKFMDYAKKVERELDDLDIR
                     VELDSRAEKIGYKIREAQMEKIPYMLIVGEKEAESDTVSVRERQQGDIGSMSVDEISS
                     IILKKIQNRENDSPQLV"
     gene            19432..20199
                     /gene="udp"
                     /locus_tag="PLB33_02290"
     CDS             19432..20199
                     /gene="udp"
                     /locus_tag="PLB33_02290"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOG62380.1"
                     /translation="MGTVYSDAQYHINLKEGDVGRYVLLPGDPKRCGKIAKYFDNPVL
                     VADKREYVTYTGYLDGVKVSVTSTGIGGPSAAIAMEELANIGADTFIRVGTSGGINID
                     VTGGDLVIATGAIRMEGTSREYAPIEFPAVADYGIVNALVKAAENLNKKYHVGVVHCK
                     DSFYGQHAPESKPVSYELTNKWGAWLKMGALASEMESAALFTVAAYRKVRAGSVFLVV
                     ANQERRRLGLDDPQEHDTEGAIVTAIEALRILIKEEK"
     gene            20354..21799
                     /locus_tag="PLB33_02295"
     CDS             20354..21799
                     /locus_tag="PLB33_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG62381.1"
                     /translation="METTKKSTNYSFALDLVEDGKDIHIKEIKKNQLPPNVTSRMLYK
                     DIVKIAWPSVVELTLTQLASMVDLMMVGGLGAWALTSVGLTIQPKFLLMTTFMALNVG
                     ATAMVARYKGAGDCKRANLILRQALMITFILSLISSLLGYIFAEEFILFMGAAEEKTL
                     AGGTVYFKIQMVGFAFMALTTTITATLRGVGNSRTALTYNLIANVVNVVLNYLLIEGN
                     FGFPRMEVAGASLATIIGQLVAFVFALIYIMKGDQYLHLRFTDGFRPDSRSLKQIITI
                     GTPAMFEQLLMRAGVIIYVRAIAGLGTVAYAVHQICMSIQALSFMNGQGFAVSATALT
                     GQSLGKNRPDMAHAYVHRTRRLGMSVSIILGIIFIFWGGNIVSLYNDAPAIVSQGARI
                     MMFVGFTQPLQSSQFILAGSLRGAGDTRATAIIIFITVLLIRPFLAIYLINVWNMGLE
                     GAWFAFIVDQTVRSALVLLRYNSGKWKSIKV"
     gene            21936..22427
                     /locus_tag="PLB33_02300"
     CDS             21936..22427
                     /locus_tag="PLB33_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOG62382.1"
                     /translation="MKVTLEQIDELRNRVNVSYEEAKKTLEKNDGDLIKSIIELEKKK
                     GRRNEKHHGGFTAAANKLLALRMSVKNKDGNTILNVPVVVILAAFLMAFWVVIFGLVL
                     AILTSCKITVYRDKAINVNNIKKNMQETVEKIKDKSEEIFKDDEEEPTDEQDESENEI
                     IIE"
     gene            22565..23245
                     /locus_tag="PLB33_02305"
     CDS             22565..23245
                     /locus_tag="PLB33_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG62383.1"
                     /translation="MGKKKILIVEDEQKIARFLELELKYEGYDVDIANNGREGFEKGK
                     SPDVDLIILDLMLPGLSGIEVCRRLRQTSDVPIIMLTAKDDISDKVTGLDIGADDYMT
                     KPFAVEELLARIRVLLKRKYHKTNEQDILKIGKLTLCKNNYKVEFDNENIELTKKEFE
                     LLEYLMKNKNIVISREKILDNVWGYDYYGDTNIIDVYIRYLRSKIDQKYNVSLIETVR
                     GVGYIIRD"
     gene            23256..24668
                     /locus_tag="PLB33_02310"
     CDS             23256..24668
                     /locus_tag="PLB33_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG62384.1"
                     /translation="MVKTILRKLLNLILYPFRITVKLLISLLKRLKFSIAFKISTTYL
                     ILYVIIIMTVALTTSVGYLIFELQKFEDTTAAIDSENIINSFPNFNTINVNERSYNSF
                     EIFDNNLNTYFSTGRPGRYADSIIVITEKIIFDNEYVHSESFTKNNSIYYMNINYPME
                     DMLRDAWRISFMVLISGAIGLMFFAPIVSSTSYKLIGPIKNMTEITKTITVNNINTRL
                     DVKGTQDELKELSQTFNEMMDRIESGYKTQQQFVSDASHELRTPIAVIKGYVNMLDRW
                     GKDDKAVLEESIVAIKNETENMQDLIEKLLFIARSDISTLNFTKEDFRIRALLEEIEK
                     ETKMIDTAHKLYFKFYDDATIYGDKMRIKQAVRILLDNAMKYTPPGEYIMISGFIQDE
                     YYVIKVEDTGVGIKKDDLNKIFDRLYRAEQSRSKQVSGHGLGLSIAKIIVLGHKGKIK
                     VKSTPGKGSEFKLLFPYIDS"
     gene            24771..25076
                     /locus_tag="PLB33_02315"
     CDS             24771..25076
                     /locus_tag="PLB33_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451156.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOG62385.1"
                     /translation="MNIQFIETGQVLTIKLKGELDHHSADEARVLIDEKIKNEKYNKL
                     VIDLKGLDFIDSSGIGFVIGRYKVIRKRNGVIEIINAGKKVRKILDMSGIGKIIKIK"
     gene            25086..25562
                     /gene="spoIIAB"
                     /locus_tag="PLB33_02320"
     CDS             25086..25562
                     /gene="spoIIAB"
                     /locus_tag="PLB33_02320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOG62386.1"
                     /translation="MHNNYVYIKIPSLSVNESFARAAVAAFCSSLNPTMSEISDIKTA
                     VSEAVTNAVVHAYEDEIGEIEIQCRIKGQVVEIIVEDFGCGIADVEKAREPLFTTRPE
                     LERSGMGFSVMESFMDELVVLSEKDVGTKVILRKKISEKKQKTLNEEFDDEKSEDD"
     gene            25874..26314
                     /gene="spoVAC"
                     /locus_tag="PLB33_02325"
     CDS             25874..26314
                     /gene="spoVAC"
                     /locus_tag="PLB33_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOG62387.1"
                     /translation="MKYNKDNYKQVVDEFTPKNKMLKNCVMAFIFGGAICLVGEIIND
                     SFVAWLGISDEEASPMTAVVLIGISALLTGLGWYDKLGKIGGAGTVVPITGFANSVVS
                     PALEFKREGLILGTAANIFKLAGPVLVFGYFSSFIAGLLSMLFK"
     gene            26324..27316
                     /gene="spoVAD"
                     /locus_tag="PLB33_02330"
     CDS             26324..27316
                     /gene="spoVAD"
                     /locus_tag="PLB33_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOG62388.1"
                     /translation="MKKTGKTYIIKDDVVIRDTYTIVGKKEGQGPLKDKFDMVIEDDL
                     WGEDTWEKCELKMQKIAVENLKNKNSLINENIDLLISGDLLNQITSSSFAARALELPY
                     IGVYGACSTMTLTMGLGAMIIDGGFAENVICVTSSHFCSAERQYRLPLEMGGQRHMSA
                     QWTVTGAAAVWLSKSGKGPKIKHVTFGKIVDLGVKDANNMGAAMAPAAYDSLNQHLQD
                     TGIDYDLIVTGDLGTVGKGIVNKMFNEDNKSIESKYDDCGTIIFDINKQDVHAGGSGC
                     GCSAAVFGSFLYKKLLNNEIKKLLFTATGALHSPTANLQGESIPGIAHCLGIEV"
     gene            27325..27681
                     /gene="spoVAE"
                     /locus_tag="PLB33_02335"
     CDS             27325..27681
                     /gene="spoVAE"
                     /locus_tag="PLB33_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOG62389.1"
                     /translation="MNYLMSFVIGGLICVVGQIIIDTFKKNNAYLLVFFVVTGAILGF
                     FGVYDKLVEIGHSGATVPLLGFGNSLAKGAMEEAANKGFMGALSGGVIKTAPGVAAAL
                     LFGYIVAVIFNPHGDK"
     gene            complement(27884..31180)
                     /locus_tag="PLB33_02340"
     CDS             complement(27884..31180)
                     /locus_tag="PLB33_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOG62390.1"
                     /translation="MSKEKKNNEINENNSDSINKNDKKKRTAKKKRKVIIRVTLLLIL
                     VAVIIAGGAVLGMVMGIIRNAPEIDPRNVLGTLTESSVIVDENGNVIEQIHDPNENRE
                     IVPLKQIPKYLKDAFIAIEDHRFEDHPGIDIRRIMGAVIHNVREGDPTAQGASTITQQ
                     LVKNLYLTSDKVWERKITEIYLAIKMERVLSKEQILENYLNTIPLGQSQYGVQAASYA
                     YFSKDVSDLTLAESALLAAAAKSTVKYAPFNRYNLEDISDIPEEDIVGNVFIGSVQYA
                     CVYNQNAIDRQHTILNRMLELEYITQEEYDAAMAEDMRVALNPGQTKMEGISSTPMDY
                     VKEKVVEDLIATRGMSYEEAENYLYKGGLTITSTIDVNIQKSLEQSYDNFPVLYLGAE
                     PTGDKPIAQDWRYFRWSGGEGTGMLDAGLNILNESGQLIFFAKENIMDEENNIYLNPD
                     EYSIDENGNLVINSKKFDIYSSTIDIVDAYTIDDKMNFVSHTIGALNVGNNYEIIEKK
                     GSKGTFLIPKNYLDKNKEMFNIGSDGILKIPEGYFFFQEKGIVQPQSAAVIIDYKTGK
                     IKAMIGGRQIEGSKTFNRAVDATRQPGSTIKPLSVYLAALDLGYSAAYPIDDLPKYNQ
                     SGERWPKNWYEHRNIKYWGIQTLRRSIEQSINTNAVTMLETIGLDAAINSLSRLRLID
                     QNNPDKDTFVSPQEDPYYNDVNLASLALGGLTKGFSPLAMTAAYGAIANDGVYVEPIV
                     YTRVVNTKGETILENIPETHVAYSPEVASLMKDILRTTVNPGLSYRARLPQELGIEVA
                     GKTGTTQANGDFWYVGFSPYYVGGIWVGNDNVQMKLSGDSGNNARLWSAIMTPIHQGL
                     PPATFTRNPNLIPISVCKVSGKLPGDLCALDQRGSQIITEYFIPGTQPTETCDVHVKA
                     EVCTSSNMKKSIYCPGNLVEERVFFTRDPLFDPEEKTDNYEAKKLYQQILEDRIIFTM
                     EELQQIYAGQMSVDENYQITHVLGIEVEKLGPSGFITEDYQYQVPTRTCYYHTKWHYD
                     QWLNEGDGNGNNGNGNGNNGNGHNGNGNGNNGNGDTLEDIINQIIDEFENSEEDPDKP
                     IDEILESIEN"
     gene            31453..32715
                     /gene="hflX"
                     /locus_tag="PLB33_02345"
     CDS             31453..32715
                     /gene="hflX"
                     /locus_tag="PLB33_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOG62391.1"
                     /translation="MEKERCLLVAVQLPGEEENDMEELEQLVEAAGGEVISSIVQNKQ
                     SIDNRFYAGQGKIEEIANYVKELDIDTVIFNDELSGSQIRNIEEIVEAKVIDRTNLIL
                     DIFAKRALTKEGQLQVELAQLKYSLPRLIGVNKNLSRLGGGIGTRGPGEQKLEIDRRR
                     IKEKITDIQNQLEVLEKVRETKRKKRMKDQVPIVSIVGYTNAGKSTLLNALMDNEYRE
                     EGENKKVFAHDMLFATLDTELRRVRLPGGRSAVFSDTVGFIKKLPTQIVEAFKGTLEE
                     IKYADLIIHLIDINDENLSAHKETTIELIEKITGSEIPILTVYNKVDKILNEKVSVLN
                     NGIIYISAKYKYNIQELLEAVDNKINGPKNFYDLKIPNDNIKIYYWLYENRVTENVEF
                     DGDGVSLRVLLYENEKDKYKDFIGEKDE"
     gene            32708..33352
                     /locus_tag="PLB33_02350"
     CDS             32708..33352
                     /locus_tag="PLB33_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOG62392.1"
                     /translation="MSNYKSIHKEGRDEIIINRSRFIGTACPVETEEEALEFIDKIKK
                     EFKDATHNVYAYVIGENSNIQRFSDDGEPSGTAGMPVLNVIKQENIKNVAVVVTRYFG
                     GVLLGAGGLVRAYTKSSKIALESGKIVDKKLFYDVLFKLDYTLLGKMDNELLKNNVII
                     RDKEYEEQVLFKLIVEKENLDKINALVGEISSGKAEISVGQAKYYSTKNGKIVD"
     gene            33464..34204
                     /locus_tag="PLB33_02355"
     CDS             33464..34204
                     /locus_tag="PLB33_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOG62393.1"
                     /translation="MSGHSKWSNIKNRKGKQDALRGKIFTKLARAITVAAREGGADPD
                     YNSSLATAIEKAKAQNMPNDNIDRAIKAGLAASAGENFETITYEGYGPSGTAFMVQCL
                     TDNKNRTAADIRHYFSKHNGNLGTTGCVSYLFDKKGVISIESDGIDEEELMMAALEAG
                     AEDFEAQDDSYEITTIPEDFIQVLNSLKEAGYENIKGDIMYIPQTYVKVEDEAAIKNL
                     EKLIDALEENDDVQEVYHNWDEPEEEEE"
     gene            34357..>35027
                     /locus_tag="PLB33_02360"
     CDS             34357..>35027
                     /locus_tag="PLB33_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOG62394.1"
                     /translation="MYVPYGLFDPTMLILIPAIIFTLYAQSKVKTNFAKYMRVQTRRG
                     YTGAEVARMLLNQNGLHDVPIETARGQLGDHYDPTKRVLRLSGEVYQGSSIASVSVAA
                     HEVGHAIQHANGYIPLSLRNMVFPVARFGSSAAWIFILVGLMIPNLVGLMDIGILLFG
                     AAVLFQTITLPVEFNASSRALEMLDANGYIYDEEIKGAKNVLNAAALTYVAAMASGLA
                     QLMRL"
BASE COUNT        12368 a         5155 c         6814 g        10690 t
ORIGIN      
        1 gagcgcccgg ctcataaccg gtaggtccaa ggttcgagtc cttgaaggcc cacttttatt
       61 tctaaatatt atgcccagat agctcagtcg gtagagcagg agactgaaaa tctccgtgtc
      121 cgtggttcga ttccgcgttt gggcatcaaa aaagaccctt aggtcttttt ttatttataa
      181 gttatgggga cacaccttct agatagctca ttgttgaagg aatgttccct tttttggttg
      241 tcagaatgac ggtacctgct tttcggaaca accaaatcct tcttctacga tttctttaac
      301 aatgcgattt atattgtcat aatacatctt attatttagt ttaatcggta tcaggtttct
      361 ttttaatccc tttatagaag tatagttgtc aatattatat tcatcattgt ctaaatagta
      421 aaactctctt atattattat ccttataaat attaagagga atatcaattt tcaatgtttg
      481 aggtttaact gacaatacca tgtcataatt tatgctatga ttaatataat cttttgctat
      541 aagagataaa atattgaatt tgcatttaat agaattcttt ttgattgtaa tatcctcatt
      601 attaattgtg ataataaggt tttttgtata tataacaaaa tcatacaaac taaaaatatt
      661 attcataaca aatggcaaat tatatacatc ctcgtaatta tgctttaata tcaaatatga
      721 aaattcctta cgctgctcta atctgtaagc ttcatacaat gtaatcacat ctttgccatc
      781 cagagtatca tttctatcaa tggagaaata cttttctacg gtttttagtt ttaaatctac
      841 ggtattgatt ttatatctaa atttttttat acaattatat aaatcaatct tattgaatga
      901 atcaaaaaca agagtaatgt ttaattgttt tgctttttct gctaggaaag ggatatcaaa
      961 gctattgccg ttgaaagtta ccatatattt cttcaatttt atcagctcgt ttaaatattt
     1021 aagggcatcc ggctggtctt gcttatattg acaaaatatt tgatatattc tatatttgtc
     1081 atccttataa agcaaaaaag ttatcgatat aatatctgaa aatatactcg acaaaccggt
     1141 agtttcaata tctatgaata ctgcttcctt ataaatttca tttaaataag ggttatccgt
     1201 tatattattt acttcatact cattaataaa catagttttc ctttaataat aactttaaat
     1261 ttttcctaat atttggtaaa atattagagg gtgttgtgaa tgtatataat aatttcaata
     1321 tttacagcat atgcatttgg aataatcagt tatgagttaa atctgtacgg ctattatttt
     1381 acagctcttc ttgcaatact tatttataat tcattaataa ataaaaaata tatgtacaat
     1441 gccgtaatta ttgcatttgt gatattatca tacattaact gtaattataa ttcaagctcg
     1501 gttctttctc aatacaaaaa tgaaagtatt gtagttgaag caaaggtaaa aaagcaaaaa
     1561 aagtccggca gcgaaagcta ctatgcttca gttataggca taaacaacac tcctatttta
     1621 aatgaagaaa atattattct ctatacaggt aaatataaaa attttcagga aaacagcata
     1681 ataaaagcaa gggtcaatgt tgccggcagt caattaaaca aaaataaaat gctttttaat
     1741 tatgaaaatt atttgaggag caagaaaatc aatgcaacgg tgtttgccga cgaagatgct
     1801 tatgttatcg atgaaaatta ttcgttttta aacaatatat caagcaaatt cagaaaatat
     1861 accgaaaaaa cattttatta taatttaaat aaaaagaatg cggaaataat attgtcaata
     1921 atattaggtg atgtaaacta tttggaggaa gagctttacg ataatataaa ggtaatgggt
     1981 cttgcccata tatttgctgt ttcgggaaca catattgttc tcatgtatat tttcttatta
     2041 aatgtcttta aatttgtttt aagcagaaga ttcagctggt tattgtcttg gtgtattata
     2101 tggttttacg ggtttttaat cgggtttccc ttgtcaatta tgagggcttt ggttatgttt
     2161 actctgcttt tcggctccga ggttttgtac agaaaataca attcactgaa ttccatcggc
     2221 cttgcagctc ttgtgttgac tatttacaac cctttttgga tttttgatgc aggatttcta
     2281 ctatcctttt cggcagcact aagtttcata atatacggca aatatataaa gacaaaaacc
     2341 actgttttaa gaaccgtata tatgtattta tttcttcaaa tatttacctt gcctgtaata
     2401 gtatattatt ttaattttgt ccctgtcatg ggtgttcttt ataacttgct gcttttgccc
     2461 gtatttacat ttattatgat ttatggattt attcttttga tattaaattc atttgctcat
     2521 attatattaa ttattccttt taatatatat gattatattt tgcacagcct tagatatatt
     2581 attgatattt cagataagtc tgcttttaac ggcttaataa tgcctacttt atctttatgt
     2641 caaactatat ttttctatat tacattattt tatatgatat acatacataa taataaaaaa
     2701 tgcaattgca aaaaaatcgg tatttttgca attgtcagtc tttattccat tacctatatt
     2761 gcatttccaa tgatggatga cagtctttat ttaaatattg cagatgtggg acaaggatta
     2821 tttacaacaa tcaagtataa aggattaaat atgatttgcg actgcggcag tacaactaat
     2881 aagcaaatgg gagaatacat tgctgtccct tacttgacaa aaagaggaat agcacaagtt
     2941 gatggaatat ttataagcca ttgggacgaa gaccattatt ccggcttaaa tgacatttta
     3001 aacagccata taaaggttaa taatatattc tcatcaaaca gcaatgaaaa ttatacaaat
     3061 gtgcagaaat taaatgcagg ctgtcaaatg aagtatgatg ataatttaag tatggaaata
     3121 ctgtggcctt ataaagattt atctttaaac aatactaata attcatcctt ggtgatttct
     3181 ttgaattata agggcagaag gattttgctt cccggagata tagaagagga agcggaatac
     3241 gctatttata atggtttaaa tgcttatgat atagtcttgg ttcctcatca cggaagcaac
     3301 acatcttcaa cagtgcagtt tgttgatgcc gtaaggccga aaattgctgt tttaagctat
     3361 ggaagaaatt tttacgggat tcctcatgaa gaagttatat cgaggtacag aaatacaggc
     3421 agcaagattt tttccacatt tgaggatgga gaaataaata tagttttaaa agataacaat
     3481 ttatattata atacttatat gaatgaaaaa tccgacaatt attataaatt gtatttaatc
     3541 ggaataatat acaagctgag catattttgt tttttattat tttggatgtt gaaaggtgaa
     3601 agttatgagc tttaatttga ttaaaaacat ggcagataaa aataagcttc ccaattgcct
     3661 gctgctttac gggaacgaag aatattttat tgattattct ataaagtata taaaatacaa
     3721 atatattgat aaaaactatg aggatatgaa ttatgccgaa tttgaaaagc cggcaagctt
     3781 gaaagactat tttgagtttg cctacacatt tccttttatg tcagaaaaaa aattatgcgt
     3841 aattaaggaa gccggttttt ttacatcgac gggatcctta gataaaaaag aagaagatag
     3901 actattaaat ttcattgatg agaatgaaac ttgcatatca atttttgtaa ttaaggacgg
     3961 gaaagcggat tcaaggaaga aaattgtaaa gaagctgaag gataataagg cagtatttga
     4021 gtttaacagg ctgacggaag gcgagctgac aaaatatatt acagaagaat tcaagaaaaa
     4081 caactttaaa atttcaatgt ctgatgcaaa ttatatggca aataacaccg gttatttgga
     4141 atatgaaagc acagtaagcc tgtatcatgt caataatgaa attaataaaa ttatgtctta
     4201 taataaagaa aaaaagaata taacaacttc cgatttagat ttgttaatgg ttaaatccat
     4261 tgaaagcaat atattcagat tagttgactg catatgcgaa aatgataaga aaagttcatt
     4321 tgagatattg aatgagatgc ttttaaacaa tacccccgag caatatatat ttcatatgat
     4381 tacaaggcag tacagaatgc tgtatcaata tgtaatgcta caaaaaaaag gatatagtta
     4441 caatgaaata atgagcaaaa tgaaaataaa gaattttgta gcttccaagc ttgctaaaca
     4501 atccaaaaat ttaaatatgg aaactatcga atactatatg aagaggtttt tggaaataga
     4561 cagtaaaata aaaaccggtg aaatcgacag cagagtaggt cttgagttga tatcaaacgg
     4621 gataataggt acggggacaa attaaaaaga ccaatcaggt ctttataatt atgctacttt
     4681 attaaatttc tttgttaatc tacttacttt tcttgctgca gcgttcttat gaatagtatt
     4741 ctttgcagca gcctgatata atttcttttc gcatattttt aaatattctt tagctttttc
     4801 gtagttgcct tcacttaagg ctgtttcaaa tttcttaata tatgttttaa tttcagattt
     4861 tctgctctta tttgcctgag tttttttgtt tataattaag attcttttct ttgcagattt
     4921 aatatttgcc aaaatatcca cctcctattt cagaatttcg cagtctcatt caccaatatt
     4981 atcgctaggg catctttgac agcaaatcaa ttatacagac tattaatgca aaaatcaagt
     5041 aaaaagaata aaaaatacaa aaaaactcag aataaaaatc agatgtaaat tttatataga
     5101 attttgcata tcctgagcgg cagcggagga actatgatag gaggaattat gtacagaacg
     5161 gatttggcgt atgaagctaa tgagtcactt gcaaaaaaaa tagagggtat aacattaaat
     5221 actcacagtt atagccatgg tgaagttgta gaacttgaaa taaaagataa ggaagcagaa
     5281 aaggcagtag gaaagaagat gggaaaatat attacttatg aaactaaaag ccttaaaagt
     5341 ctttcggaga aatcaagaaa tgaagttata gatatattgt caaaagctat aagggatata
     5401 tccggactcg gcagggagaa aatattggtg gtgggattgg gaaacagaaa tattacggct
     5461 gattccttag gacctaagac cctggataaa attaaagtta cggctcagta cttcaaagca
     5521 tataataagg actttgacga ggattttaac gaggtttcta tattagagcc gggggtatta
     5581 ggcacgacgg gaattgaaac gattaataca attgttggag ttgtcgaaaa aatcaagcct
     5641 tcattattaa ttatcataga tgcattagca tcacgaaaaa tgagaagact ttgtgcagtt
     5701 gttcaaataa cagatgcagg tatagagccg ggatcaggaa ttggaaatat gcagggctca
     5761 ttaaataaag aaaccatagg cacaaaggtt atagccttag gcatacccac tgtggttgat
     5821 actgccacca ttgtaaatga tacaattgaa ttgatggaga aaaaattaag caataaaact
     5881 gatgacgtag gaaagataat gggtatttta agcgatttgg agtataatga gaaacatgca
     5941 tttataaagg aaattttaag ccccatgtat ggagaatcaa ttgtaacgcc aagttatgtg
     6001 gatgaaataa ttgattcatt gtcaagttta ttggcagaat ccataaacag agcagtacat
     6061 cccggatatg taaattcgta atataattgt aaaaattaac agtgttattc aggaaacaat
     6121 acacatatac attaatatat tactttatag atattttatg gagtagtata ttactatgaa
     6181 gaaaatatgt ggagttgata tcatgaagag acgaagaaaa aacaaggatg gaaggtcttt
     6241 ttacatggtg atgtctatat tttgtttgac agcacttttt ttgggataca gttttgtgta
     6301 caatttatct gaggagagga agacaggtaa agaagttacg acgattttca ttaattataa
     6361 taccgataaa agtaaagacg gaaatttgag tacatttgat aaggttatgt cttatttcaa
     6421 cttatttctt gaaaaaactt ttaaagggaa aaagagtaat gatgtagagt taatcgagaa
     6481 cattgataca caatcttatg aaaaagatat tgttagaaca atagccagcg aggatacgaa
     6541 gaaagatttg gaaattttta atgaaagccg gtatgaacct gaatctgaca gtgaagtata
     6601 cctgacaagt aaacgaaaag agaatttttt taaagttatt tcaaaaacat caagaagcag
     6661 cgctccaaga agaaaattat tcataaacgc tgcttctgtt attaataatt ttaatattgt
     6721 gttgtttcac actcatggaa ccgaagcatt tgaagaaagt tatgaaacga attaccgctc
     6781 cttggatgaa aatttaaata ttttggggat aggagcaaag atttcaaata atttgcttaa
     6841 taaaggcatt aagaatactc acttaaaaga ttacaatgat tatccggatt acaactcatc
     6901 atatgcaaat tcaaattatg cggtaagaca agttttgtca gacagcaaaa aaaatatact
     6961 aattgattta caccgtgacg gtgccgaaga gaattctaat tatgaagcaa ttctttcgga
     7021 agtgaaaact accgagataa acggtaaaaa agctgcaacc tgtaccctgg ttgtggggaa
     7081 taagaattca aacgcttcga aattaaaaga aaatgcagga agactgtatg aaatagctta
     7141 tgaaatgtat ccgggactta tgcgaaaaat catttttaga gagggtgcat attttaatca
     7201 gtatttatca gattattctt tcctcataga ggtgggatgc acattaaata catatgaaga
     7261 aattgaatat accgcggatt tattaactga agtattatat gagtatatta atgaaattga
     7321 ataataatct tttacaatta cgtcaacatt tgatataata cccatagctt aaatttggga
     7381 gggtaactta tttggacaga aaaaaatata taaggaactt ttcgataatc gcccatatag
     7441 accatgggaa atctacatta gcagacaggt tgatagaaaa tacggggctt cttacacaca
     7501 ggcaaatgca ggagcaggtt ttggacaata tggacctgga aagagaaaga ggaattacaa
     7561 ttaagctcca aaccataagt ttattgtaca aggctaagga cggaaataat tacactctta
     7621 atttaataga tactccggga catgtcgatt ttaactacga agtttcaagg agcttagcag
     7681 cttgtgaagg agctgtttta gtcgtggatg cagctcaggg cattgaggca cagacactgg
     7741 caaatgtata tttggcctta gaccaggacc ttgaaatagt tcctgttata aataaaattg
     7801 atttacccag cgcaagaccc gatgaaataa aacaagaaat tgaagatgta ataggtctcg
     7861 attgctctga tgcacctctt atttcagcga aggacaatat aaatatcgaa caagttttag
     7921 aagccatagt aacaaagata ccgcctccga caggcgacga agatgcacct ttaaaagctt
     7981 tggtatttga ttcctattat gacagctacc gaggtgttgt ggcatatata agaatcaaag
     8041 aaggtcaggt taaaaaggga gacaaaataa aaatgatgtc aacaggtaaa acctttgatg
     8101 tcacagaggt tggaataatt tcacctacac agaagccggt accttgtctt tattcgggag
     8161 atgtgggtta tttgtgtgcc agcatgaagg aagtaaaaag ctgtcaggtt ggagatacaa
     8221 taactaataa tgcaaatcct gcaaaagagc ctttgccggg atataaaaag gttacgtcca
     8281 tggttttctg cggtatttat ccggcagagg gagaggaata caacagtgtg agagatgcat
     8341 tggaaaaact tcaggtaaat gatgcttcct taatttttga gccggaaaca tcagttgcct
     8401 taggatttgg attcagatgc ggatttttgg gtcttttgca tatggaaata attcaggaaa
     8461 ggcttgaaag ggaatttgat ttaaatatta tagcaacaac tccaagcgta atatacaaag
     8521 tatataagac tgacggagaa atgatggttt tgcaaaatcc tacaaatatg cctcccgtac
     8581 aggaaatcga atatatggaa gaaccgatag ttgctgcatc cataatgagt cctaccgaat
     8641 atgtgggagc aattatggag ctatgccaaa accgcagagg tacatttgta aatatggaat
     8701 atatggaagc gaaaagagtt attctacatt ataaattacc tttgaatgaa gtaatctatg
     8761 atttctttga ttccttaaaa tcaaaaacaa gaggatatgc atcattggat tacgagcttg
     8821 acggttatgt acgctccgaa ttggttaaaa tggatattct aataaattcc gaattggtag
     8881 atgccttttc aataattgtt cataaagata aagcttatga aagaggaaga gcaatagttg
     8941 aaagacttag agatgtaatc ccgaggcatt tgtttgcagt gcctcttcaa gctgcaatag
     9001 gaaataaaat aattgcaaga gagaccgtaa aagcactcag aaaagatgtt cttgcaaagt
     9061 gttacggcgg agatataacc agaaagaaaa agcttttgga aaagcaaaaa gaaggcaaga
     9121 aacgaatgag acaaatagga atggtggagg ttccacagga agcattcctt gcagttttga
     9181 aatttgatga agattaagta tgccctaacc ccatttatca gagtattttt tctgataaat
     9241 ggttggtagg tcaaccgcac agaatgccaa atttgtgcga cgaaaggagc aaacaaattt
     9301 gatgcattcc cctgcgaggg ctttgtgccc ttttttatta tgacaactta cctaaaaaaa
     9361 tgatttcatc ggtatcagca ttaataatag taagaccctt ttcttcccaa tttgatgagc
     9421 cgagataacc caacctgtca tcccatgcat cgtcgtattc gtcttttgat accaattcat
     9481 tatcaacata gtattcaact tctgttttaa ttaatttaaa accatcttgg ctaaaatagt
     9541 tttgttcttc atcatcaaaa tcataataat cttcatcata atcaaaatca tcataataat
     9601 tatcatcttc attttcatca gtatatagat aaccgtaatt tatcaaattg tacaaacatt
     9661 caaacatatc atcaagtgta tcattgtttg catatgcatc gcccaatttt acaaaaaaat
     9721 ccaatatttc cttattttta atttcatatg ttactatatc cttggtgtaa agcatttctt
     9781 tccaatctcc gctttcactg attattgtta ctctcatatt actccccctt gccaccataa
     9841 ctgttagtaa aattttatgc gcccaaatac atattatgtt tatgattagt gaaaacagag
     9901 aataaatatt ttgtttgtta tttgaattga gtcaaaaaaa aaggtcattc tatgaataac
     9961 tttcattaga aggcaacgac aagaacgggc tatccataga aatgacaatg aagaagataa
    10021 tactatatat ttgcatacaa tatttcattg gggtgcttga tttctattat atttgtgcca
    10081 tgcccggcaa ttgcattttt aaagtctgat gtaactttgt aatcatccca aaaatgcatt
    10141 ggaataaaca gcttagggtt taatttatca ataaagtatt gagcgccgca caagtaatta
    10201 ttttcaagcc ttctgtcaac aggaaagaat gcaatatcaa ttttcacgcc gtgctttaca
    10261 atatctgcca taatgctttt gaaattatct tccatagttt tttcttcttc aggcgtatca
    10321 tcataccatt tccaccagtt taaatcaccg gcatgaaata agtttaaacc gtctatattg
    10381 accataaaac taactccttc atcagtagaa ccaaaaatat tcagttttat ttcattgatt
    10441 gtaagttcgt ctcttggtct tactataaat agattatcaa ttttattata aatttttata
    10501 tcagaggata atatgtaata tgtactcggt tttttattta tccaggataa aacattataa
    10561 ttaaaatggt cttgatgtga atgagagctg aatatataaa gagatttagg gcttgagaat
    10621 acatctttga gcaatttaga aaaatcaaaa tcagttttat catttttgtg attgaaataa
    10681 tcaaaaagca aaaatgaatt cattgtttct atcagaaaac agctatggta aatatatcga
    10741 attatcataa tacctccaca aatatatatg agtttcttaa ttataatata atcctaaatt
    10801 atgagcaata ctataattaa atataactta agacagtatg ctcataaatt tttgtaatta
    10861 caaaataaat gtttgtaatt attaaatccg cattatataa tgagaagttg aaaaaaatgg
    10921 aaagaggtaa aaaaatcaat gaaaattgaa aatatagttt taaataataa cattcaaaga
    10981 gctttggatg atatgggctt tgaagaaagc acccaaatcc agcaagaggc aattccttta
    11041 atattggaag ggaaagatgt tgtaggacag tcaaatacgg gaaccggaaa aacagcagct
    11101 tatgcaattc ctattcttga aaaaatagac aaagaattga gagtgcccca ggcattaatc
    11161 cttctgccaa cgagagagct ggctgtccag gttgccaatg aagtaagaaa aataggcaaa
    11221 tatatggaag gtatcaaagc tgtaacagtg tatggaggag ctgatatcag agaacagata
    11281 aataaactta agggaggagc tcaaataatt gttggaaccc ccggcaggat tatagatttg
    11341 attgaaagac atgtaataaa acttagcgag ctaagaattg ctgttttgga cgaagctgat
    11401 gaaatgctta aaatgggatt tagagaggat atagagctta ttttaagcaa aattgaccat
    11461 cccgttcaat ccttattgtt ttccgcaaca attcccgatg atatcagaaa aataattaag
    11521 agatttctta attcccctgt ttacgtgaag gttttaagag aaggaataac agccaaggaa
    11581 gttaaacaaa gttatttttt ggtgaagcat tccgataagg ttgaagcact ttccaggtta
    11641 attgacacct atacaccaaa acttacattg gtattttgca atacaaagaa ggcagtggat
    11701 gagttatata acaagcttat cgataaaggg tataactgcg ataaaattca cggagatata
    11761 aaacaatcac aaagattaga tactttaaac aagtttaaca atgggcttat tgatatactt
    11821 atagctacag acgtggctgc aagaggcctt gacataaaag aagtagatct tgtaataaac
    11881 tacgaagttc cttcaaaaga agattattat gttcaccgta taggcaggac ggggagggcc
    11941 ggaagagaag gagcatcatt taccttggta agtgcaaaag aattaagaaa aatagaggat
    12001 attgaaaaat ataccaaaaa atcaatccga aaaagagcaa tacccacagt ggacagggta
    12061 aatgaagtta aacaggataa atttataaga gatatagtaa atgtaataga aagcggagat
    12121 ttaggggaaa acagagaact ggcagaaaaa cttatggata agggctatga tgccgagact
    12181 gtaattgcag ctatgattaa aaagcttgtt aaatttgatt tatcggaaga gagagattta
    12241 aatgaagtta ttcccgaaag aaaaaagaga ggcggaaagc ttgaaaatac agaaagattc
    12301 catattaatc tcgggaaaaa gcaagggatt cgaccgggtg acatattagg agcagtagca
    12361 ggagagtgcg atattcccgg ctatgatatc ggagaaatag atgtactgga taatttttca
    12421 ttctttgctg cttcaaagga gcataaaaaa agaattcttg atagaatgaa ccgctcgcaa
    12481 ataaaaggaa tggatgttgt tgtagaatta tctaaagaaa aaaaatcacg atttggaaga
    12541 gataaatcat cagatgacaa ctcatcccga aaaagaaaga aacgaaaatg attaactaac
    12601 ccatctttat gcagtaataa aaatgtgact tatatgatga cctaagtcac tttttatttt
    12661 atttttcttc tcatataatg aattcataat aaaagcgctt ttaagagcaa caaaaaaata
    12721 aaaaaattct tgtaataaat atgcatatat ggtataacaa ttaatggaaa atattaaagc
    12781 taatgtattt ttttggcggt gttttaatga atattattgt taaatttata acaaaaagta
    12841 ttttagaaaa aaagttcagg tcttttataa ttattttctc agttacaatt tcagcagcct
    12901 tattttttgc ttcaagtgct ttggcaggta ccatgagttc catgtatgaa gctcttatta
    12961 gaatgcagac cggcaaagca gatttattga tttatcccgg cagggaatca ccttcttcca
    13021 cttttaaaat attgcccaat caagtaaagg gtgctgatta tattgtagga actttttcgg
    13081 ttggcggaat ttataaattg tcagaggaag aatatcaaat tgaaagaaaa aagacagagc
    13141 tgctatatat aaaagggttt aatatagatg aattggaaaa acttaatccc ataaattatg
    13201 gtcaaaaggc aacaggcagg gagttttcag acaatcatat tattttaagc gaaatatttg
    13261 caaaaaaata tgattataat acgggagaca gtatacacat agaaatcggc ggtgttgaca
    13321 gagttttaac cgtatggggt atttcaagac ctacaggtgt atttcagcat aatccgcagt
    13381 caaattctat agccgcagta atgcctctag atactcttac ttctttatat aatcggagag
    13441 gttgggttga tactgcttat attgttgttg aagaaggata tgatatttta aatgtaaaag
    13501 aagaaataag caccttgtat agcagatatg ttgtagagga gccttttaca gcggaagttt
    13561 tgaatgagta tctgcaattt ttagttgtac ctttgtattt aatgaccatt atggtgttat
    13621 ttatcagtgt ttttattatt tattccacat ttaaggtaat aacaacagaa aggcttcctg
    13681 tcataggtac ttttcgaagc attggtgcta caagaaaaat gacggatatt atactcatag
    13741 gcgaaagctt attgtatgga attgtggggg gaatattagg agtattaaca ggaatgggag
    13801 ttttatatct tattgcccga ataatggctt cagaccctta taacggacag ttggatgtat
    13861 cgattaaatt tggcttagtc catatgctga cgacctttat tttagccctt atggttgcaa
    13921 ctgtcagttc ttggattcca ataagtaggg cttcaaaaat accaataaaa gaattggtat
    13981 tgaatttgat tgatagagaa ataatcaaaa aaaacaaaaa ggtcattata ttgactgtta
    14041 tgtttgctat atcgataatt ttatctttca taacacaaaa tatagcatca atgatattca
    14101 atcttttgtc tttattgata atatttgctc ttgtaataat ctatattcct tttattacat
    14161 catttttatt aaagtttttt cagcaagtct atggagctgt atttggaaat gaaggaattc
    14221 ttgcggttaa aaatttaaag gataacaaaa atatatataa taatatatca ttgctggcaa
    14281 ttggaatttc ggttattctc atgattaata taatcagtta cagcgtaggt atagaagtgt
    14341 taaatgctta taaggactgg aagtttgata taatgctttc cttaaacggc gatagaaata
    14401 cggaacaggt acttagaagt attgacggag tggaaggaac ttacggagcc tttgaaagtt
    14461 ttcaaggaat aaaagttgtg gataccgatt acagcattgg atatatgcag ggtattgata
    14521 tcatcaagta cagggattat gcagcttttc gttcggaggg caatgaagat gttgacgagc
    14581 tgttcaaaaa gcttgatgac ggcagaaata taatggttac tcttatgatg aaagacagcc
    14641 tgggattaaa aataggcgac aaacttacat tagatatgaa ttccggcgaa aaaacctata
    14701 atataatcgg tttttacgat tcaataatgc aaaacgggag taatgcaatt atatctcaga
    14761 aatattttaa aacagatatg gagcagccat attataacag attttttgta aaaacatcta
    14821 aggagccgga gttagtccta tcagaaatca aggaaaagtt tatgagacag ggcgtatggg
    14881 gtaattcaat ttcaaacatg gaaaaaacaa attatgagca aaatagtcaa ttcatgatta
    14941 tccttcaggc attttctctt ttagctatgc tcataggtat cttcggggtc tttaacaatt
    15001 atatgattag ttttttagag cgcagacgtt atattgcaat taagcgttcc ataggactga
    15061 gcaaaaagca aacattgaaa atgatatttg tggaggcttt gacaggcgga tgtattggtg
    15121 caacagttgg aattataggt gcaatgttca tgctttggat agtacctgaa ttgatgcaga
    15181 caataggtgt tcccttagcc atacattata agtcagattt atttgcagcc tcattattta
    15241 caggaattat aatctctgtt attgcttctg taagtcctgc attaaaaaca actaagttag
    15301 acattatcga atcaattaaa tatgaatagg ggatagttat ggagaaaatt attgaggttg
    15361 aaaatttaaa taaaacgtat atgttaggaa atgtacccgt agaaattttg caggaaataa
    15421 actttacaat aaacagggga gagtttataa caataatggg tccttcaggt tcaggtaaaa
    15481 gcacattgct atacttgttg ggaggattgg ataaacctac atcagggaaa atacttataa
    15541 ataataagga tatttccgca ttgagtgatg aggagcaaag tacattaaga cgaagaataa
    15601 tcggattcgt atttcaattt tacaatttag ttcctaacct gactgtagaa gataatgtta
    15661 tgctcccggt attattggac gggaagaaga ttaaggatta ccgggaaaaa ttggatgaaa
    15721 tacttgaaac agtaggattg tcagacagaa taaatcatac tcctagagag ttatccggag
    15781 gacagcagca gcgtgttgca atagctcgtg cattaattaa tgagccggat attatattag
    15841 ccgatgagcc tactggaaat ttagacagta aatcatcgga agaagttatg aaactgttac
    15901 aaagaataaa taaagtgaaa ggaaaaacca tattacaggt aagtcattcg ctggaaacgg
    15961 ctaaatatag tgatagaatc attaatgtaa gggatggaaa ggtgtggtgc aatgagaatt
    16021 aaagcaatat tgatgcttct tgttataata ataagtgttg ccggctgcaa agctgaagac
    16081 ccgcagacaa ctgttgagga aatagattta tcccaagaaa aaaaatcaat tgaagccttt
    16141 ggagtagtta aaccggagga attcatggat attataattg attttaattc cgtggttaag
    16201 gaagtttctg taaatgaagg acagtatctg aaattgaacg aaccaatact cttattggat
    16261 ttgtcacaat atgaagcaga tatatcagac actgtaagcg aattaaatat tgctgaattg
    16321 gaatatcaac aaatcagtat gggatttgaa gatttaagcg ctgaagttaa caagcttaaa
    16381 aataatataa aatataccga aggcttgcat caaaatacaa taaaagaact tgacataaat
    16441 aaaaagctct ttgaagaagg agtaatttct gaggaaaaat ataatcaatc aaagttaaaa
    16501 gctgaagaag cagaaaataa tttagagaac ctcaagtttg agttgagcaa tgcagaagat
    16561 gcaatttata aaaaaactca atcagaaaag gcaaaatatg ctgttcaatc ggaatatata
    16621 gctcaaatta aaaacagatt aaatatttta caaaataaat taaataaacc gtatattgca
    16681 ggaaatcaaa ttgtttccat gtatgataat gcagtagtat acgatataca atatcaaccg
    16741 ggacatataa ccgatgtttc aaaaaaggct ttttcaatag caaatcttga cagcttaaca
    16801 attgaggcgg aagttgttga agagtttatt aaagatgtac gggaaggctt aaaggtaaaa
    16861 atagttccgg ctgcggacag gactatagaa tgtgagggtc gtgttatcta tgtttctgat
    16921 attgcttaca gcaaaaatgg cgggacagta gtgccaatta aaatatcatt agataataat
    16981 gattcatttc tgaagcctaa ttacaatgta gatgtctata ttgaagtaca gtgaggatat
    17041 tttaaagatt aaaaaatata aatttactgt tgacaaaaaa atttaccggt aatataatgg
    17101 tcaaaatgaa ctgtgatgga tcgagtagaa cagacacagc ttaacagaga gggaatgttg
    17161 gtggaagttt cccatgtatg actgtttgaa aactaatccg gagttaagac agaaagtcaa
    17221 atggcgtaat gtcttggtat ggatgcctta aatcctttga gaagcaacta aggtggaacc
    17281 acggtatctc gtccttaaac tatgtttagg aacgagattt tattttatta acgactgtca
    17341 tcctgacggg ttattaatag caattacact aatttctaag aggaggaata gttatgatta
    17401 agattaaatt tcctgacaaa agcgtcagag aatatgaaaa aggaattaaa gctttggatg
    17461 ttgccaaaag tatcagtgaa ggattggcaa ggaatgtggt gggagccgta tataatggag
    17521 aaacaatcgg acttcaaacc gcattaaacg aagatggaaa tattaaattt ctgaaatttg
    17581 aggacaagga aggaaaagat attttctggc atacttcatc tcatattatg gctcatgcca
    17641 ttaaaagact gtggccggag gcaaagcttg ccataggacc tgcaattgaa aacgggtttt
    17701 attatgatat tgatttagaa caccgatttg tgcaggaaga ctttgaaaaa atcgaagctg
    17761 aaatgaagaa aattgtaaaa gccggctatg aattagaacg ttttgaactt cccagagaag
    17821 aagcaattaa gtttatggaa gaagcaaaag aagattacaa agtcgagtta attaacgact
    17881 tgcctgaaga tgcggtaata tcattttaca agcatggtga ttttgttgat ttgtgtgcag
    17941 gacctcatct tgaaagcaca aaaaaaccaa aggcttttaa acttctcagc attgcaggag
    18001 catactggag aggaaatgaa aacaataaga tgctccagcg tatatacggt atttcatttg
    18061 aaaaggcaaa agacttggaa gattatttga aaatgttgga agaggcaaaa agacgtgacc
    18121 acagaaaact tggaaaagaa ttgaatttat tcatgatgtc cgaagaaggt cctggatttc
    18181 ctttcttcct accaaaaggc gttgtagtaa aaaatgaact tatgaaatat tggagaaaac
    18241 ttcataaaaa agcaggttat gttgaaatag aaacaccttt aattctaaat cagcatttat
    18301 gggaagtatc cggacattgg tacaattata gagaaaacat gtatattact tcaatagatg
    18361 agcaagactt tgccattaag cctatgaact gcccgggtgg aatcttagca tataagaatt
    18421 ccatgcactc atataaagat ttccccatga gagttgcaga agcaggaaga gttcacagac
    18481 atgaattatc gggtgctttg cacggcttga tgcgtgtaag agcatttaca caggatgatg
    18541 ctcatttata tatacttcct gagcaaatta aggatgaaat taaaggcgta ataaagttaa
    18601 tcgatgaaat ttataagcaa tttggtttct catataaatt ggagctttca acaagaccgg
    18661 aaaagttttt gggagaaatc aaggactggg atttagcgga agcatctcta aaagcagcat
    18721 tggatgagtt gaatttagat tatgttataa atgaaggtga tggagctttc tacggaccaa
    18781 aaatcgactt ccacttaaca gactgcatag gaagaacctg gcagtgcgga acaattcaac
    18841 tggattacca gcttcctcag cgctttgaaa tggaatatgt gggagcagac gggcttaagc
    18901 acagaccgat aatgattcac agaaccgcat ttggaagtat tgaaagattc ttcggaattc
    18961 ttattgagca atatgcagga gcattcccga catggcttgc accggtgcag gtaaaaatac
    19021 tgcctatttc ggataagttt atggactatg caaaaaaggt tgaaagagaa cttgatgatc
    19081 ttgatataag ggttgaatta gactcaaggg cagaaaagat aggatataaa atcagagaag
    19141 ctcagatgga aaaaattccg tacatgttga tagtagggga aaaagaagct gaaagcgaca
    19201 ccgtttccgt aagagaaaga caacaaggag atattggaag catgagtgtt gatgaaataa
    19261 gcagcattat tttaaaaaag attcagaata gggaaaatga ctctccacaa ctggtttgat
    19321 tgaaaaatga ggcatgcaat ttgcagcctc ttttttataa taaaaaagat attgaatttt
    19381 accaatattt tatatattat gtagataatt aaaattatgg aggtatgcag catgggaaca
    19441 gtatactcgg atgctcaata tcatataaat ttgaaagaag gagacgtggg caggtatgta
    19501 ttattgcccg gagatcccaa aagatgcggt aaaattgcta aatactttga taatcccgtg
    19561 ttagtagctg acaaaagaga atatgttaca tatacaggct accttgatgg agtaaaggta
    19621 agtgtgacat caacaggaat cggaggacct tctgctgcaa ttgcaatgga agaactggca
    19681 aacataggag cggatacatt tataagagtt ggtaccagtg gaggcataaa tattgatgtt
    19741 accggcggcg acttggtaat agccacagga gcaatccgta tggaaggtac aagcagagaa
    19801 tatgccccca tagaatttcc ggcagttgct gattatggaa ttgttaatgc attggtaaaa
    19861 gcagcagaaa atttaaataa aaaatatcat gtaggtgttg tacattgcaa ggattctttt
    19921 tacggacagc atgcaccgga gtcaaaacct gtaagctatg agttgacaaa taaatgggga
    19981 gcatggctga aaatgggggc cttggcatct gagatggaat cagcggcatt gtttacggtt
    20041 gcagcttaca gaaaggttag ggcaggctct gtatttcttg tagtggcaaa ccaagaaaga
    20101 agacgtctgg gtcttgatga cccgcaggag cacgatactg agggagctat tgttactgca
    20161 attgaagcgc tgcgaatttt aataaaagag gaaaaataat accggattta ttgaatttta
    20221 ttggtagctt tagcagccgg ttttgttgac attgcaaaca aatcatgata tcataaatta
    20281 taacgaattt tagcggctca aaccgcaaaa aagtcgcatc tatgcgactt tatttatgcg
    20341 acaaggagca acaatggaaa caacaaaaaa atctactaat tatagctttg ccttggactt
    20401 ggtagaagac ggcaaggata ttcatataaa agaaattaaa aaaaatcagc ttcccccgaa
    20461 tgtgacttcc agaatgcttt ataaagacat tgtaaagata gcatggccat ctgttgttga
    20521 gcttacctta actcagcttg catcaatggt agacttgatg atggttggag gtttgggagc
    20581 ctgggcactt acttccgtcg gtcttacaat tcagcctaaa tttcttctca tgacaacttt
    20641 catggccttg aatgtcggcg caactgcaat ggtggcaaga tacaaaggag caggagattg
    20701 taaaagagca aacttaattc ttaggcaagc cctgatgatt acttttatac tttctttgat
    20761 ttcttctttg ttaggttata tttttgcgga agaatttatc cttttcatgg gtgcagccga
    20821 agaaaaaaca ttggcaggcg gtacagtata ttttaaaatt caaatggttg gctttgcatt
    20881 catggcattg accacaacaa tcacggcaac attaagaggt gtcggaaact caagaacagc
    20941 attgacatat aatcttatag ctaatgttgt aaatgtagtt ttaaattacc ttttaattga
    21001 agggaatttt ggttttccgc gcatggaagt tgcgggtgct tctttagcaa ctattatcgg
    21061 acaacttgtt gcatttgttt ttgcattaat ttatattatg aagggcgacc agtatttgca
    21121 ccttcgcttt acagatgggt ttaggcctga ttctcgttcc ctcaagcaaa taataacaat
    21181 aggtactccg gcaatgtttg aacagctgtt gatgcgtgcc ggagtcataa tatacgtaag
    21241 agcaattgcg ggtcttggta ctgttgcata tgcagtccat caaatatgta tgagtattca
    21301 ggcattatcc tttatgaacg gtcagggttt tgccgtgtca gctactgcac ttacgggaca
    21361 aagcctgggt aaaaaccgcc ccgatatggc acatgcctat gttcacagaa caagaagact
    21421 tggaatgtct gtttcaatta tattgggaat aatctttata ttctggggcg gtaatatagt
    21481 atccttatac aatgatgccc cggcaatagt ttcacaaggt gcaaggataa tgatgttcgt
    21541 aggatttacg cagcccttgc agtcttccca gtttattttg gcaggctcat taaggggtgc
    21601 cggagataca agagcaactg caataattat ttttataacg gtacttttaa taagaccttt
    21661 tttggcaata tatttaataa atgtatggaa catgggactg gaaggagctt ggtttgcttt
    21721 tatagttgat caaactgtac gctcggcact tgttctttta aggtataatt caggaaagtg
    21781 gaaaagtata aaagtctgaa aattcatact tttcatattt cttaaaatta tttttcatat
    21841 tttaatctaa ttttaattta tacctaaaac ttaattaata ctttggtggt accttatata
    21901 taagaaaaaa ataaaattta tatataggag gaactatgaa agttacattg gaacaaatcg
    21961 atgaactcag aaacagagtt aatgtaagct acgaggaagc taaaaaaacc ctcgaaaaaa
    22021 atgacggaga tttaattaaa tcgatcatag agcttgaaaa gaaaaaagga agaagaaacg
    22081 aaaaacatca tggaggcttt acagcagccg caaacaagct tttggctctt aggatgtctg
    22141 ttaaaaacaa ggacggaaat acaattttaa atgtgcccgt ggtagttata ttagctgcat
    22201 ttttgatggc attttgggtt gtaatcttcg gattggtact ggcaatattg acatcctgca
    22261 aaataactgt ttacagggat aaagcaataa atgtaaacaa tataaagaaa aatatgcagg
    22321 aaacggtgga aaaaataaaa gacaagtcag aagaaatctt taaggatgac gaagaagagc
    22381 caacagacga acaagatgaa tcagaaaatg aaataatcat tgaataaaaa ataatatcag
    22441 gtcttaggcc tgatattatt cattaacgac ataatgtact tgtaatagag aagatataag
    22501 aatagtaaaa actaattgat aaaaaaggaa aaaaatagta caataaatca aaaacgaggt
    22561 tattatgggt aaaaaaaaga ttttaattgt ggaggatgag caaaagattg cgcggttttt
    22621 ggagcttgaa ttaaaatacg agggctatga tgtagatatt gcaaacaacg gaagagaagg
    22681 atttgaaaaa gggaaatccc ctgatgttga tttgataata cttgatttaa tgctgcccgg
    22741 attaagcggc attgaagtgt gcagaagatt aagacaaaca tctgatgtac ccataataat
    22801 gctgactgca aaggatgata tttctgataa agtaacaggg ctggacatag gtgccgacga
    22861 ttatatgaca aagccctttg ccgtagaaga attattggcg cgcatcagag tattgttaaa
    22921 aagaaaatac cataaaacca atgaacaaga tatattgaaa atcgggaaac ttacactatg
    22981 caaaaacaat tataaggttg aattcgacaa tgaaaatatt gagcttacca aaaaagaatt
    23041 tgaattattg gaatacctta tgaaaaacaa aaacatagta atatcaagag aaaagatttt
    23101 ggataatgtt tggggatatg attattatgg agacacaaat attattgatg tatatataag
    23161 atatttaaga agcaagatag accagaaata taatgtaagt cttattgaaa ctgtcagggg
    23221 agtaggctac ataatcagag attgaaggaa gacaaatggt aaagacaatt cttagaaaac
    23281 tgttaaactt aatactttac ccatttagaa taaccgtaaa attgctaata agtctgttaa
    23341 aaaggcttaa attttcaatt gcatttaaaa tttctacaac atatttaatc ttgtacgtta
    23401 taataattat gacagttgcc cttacaacct ctgtaggcta tttaatattt gaactgcaga
    23461 aatttgaaga tacaacggca gcaatcgatt cagaaaatat aataaacagt ttcccgaatt
    23521 ttaacactat aaatgtaaat gaaagaagct ataattcttt tgaaatattt gacaataatc
    23581 ttaacacata cttttcgaca ggaagaccgg gaagatatgc ggacagcatt attgtcatta
    23641 cggaaaaaat aatttttgac aatgaatatg ttcattcgga aagctttaca aaaaacaatt
    23701 ctatttatta tatgaatata aattacccga tggaagatat gctcagggat gcgtggagaa
    23761 taagctttat ggttttaata tccggagcca ttggtcttat gtttttcgca ccaatagtat
    23821 caagcacaag ttacaaactc atcggtccca taaaaaacat gactgaaatt accaagacta
    23881 tcacagtaaa caacataaac accaggctcg acgtgaaagg aacacaggat gaattgaagg
    23941 aattgtcgca aaccttcaat gaaatgatgg ataggataga aagcggatat aaaacacagc
    24001 agcagtttgt ttcagatgca tcccatgaac tcaggacacc gatagctgtt ataaagggct
    24061 atgttaatat gcttgacaga tggggcaaag atgataaagc agtgcttgaa gaatctatcg
    24121 tagcaataaa aaatgaaacc gaaaatatgc aagatttaat tgaaaaatta ttatttattg
    24181 caagaagcga tataagcact ttgaatttta caaaggaaga ttttagaatt cgtgcccttc
    24241 ttgaagaaat tgaaaaagaa acaaagatga ttgacacagc gcacaaactg tattttaaat
    24301 tttatgatga tgcaaccatt tacggcgata agatgaggat taaacaggct gtgagaatac
    24361 ttctcgataa tgccatgaaa tatactcctc ccggcgaata tattatgatt tcaggcttca
    24421 tccaggatga atactatgtt ataaaagtag aggacaccgg tgtaggaatt aaaaaagatg
    24481 atttaaataa aatatttgac agactttata gggcagagca atcaagaagc aagcaagtaa
    24541 gcggtcatgg actggggttg tcaattgcta aaataatcgt attaggtcat aagggtaaaa
    24601 taaaggttaa aagcacaccc ggaaaaggtt ccgaatttaa attacttttt ccttatattg
    24661 acagttgatt taaatttacc ataatcgaca aaaaacatta aaataagtca aggaaatatc
    24721 ctttccctag tagtataata gcttgtacat tatgcagaaa ggatgatttt gtgaatattc
    24781 agtttattga aacgggacaa gtattgacaa tcaaattaaa gggcgagtta gaccatcaca
    24841 gcgccgacga agctagggta ttaattgatg aaaaaatcaa aaatgaaaag tacaataaat
    24901 tggtaataga tttaaaaggt cttgatttta tcgacagctc aggtatcggc tttgtaatcg
    24961 gtcgatacaa agtcataaga aagcgtaatg gtgtaataga aataataaat gcaggcaaaa
    25021 aggtacgaaa gattttggac atgagcggca ttggaaaaat aataaaaata aaataggggg
    25081 acaaaatgca taataactac gtatatatta aaataccaag tctatctgtt aatgaatcct
    25141 ttgcaagggc tgcggtcgca gcattttgct caagtttgaa tccaacaatg agcgaaatat
    25201 cagatattaa gacagcagtg tccgaagcag taaccaatgc agtggtacat gcctatgaag
    25261 atgaaatcgg agaaattgaa attcaatgca gaattaaggg gcaggtagtt gaaataatag
    25321 ttgaggattt cggctgcgga attgcagatg tagaaaaggc aagagagccg ttatttacca
    25381 caagaccgga gcttgaacgt tccggaatgg gtttttccgt tatggaatca tttatggatg
    25441 aattggttgt tttgtcagaa aaggatgttg gaacaaaagt tatattaaga aaaaaaatat
    25501 ctgaaaaaaa acaaaagaca ttaaatgaag aatttgatga tgaaaaatca gaagatgatt
    25561 aataaataat taagcgaaat agaaattttt atcttgaaca aatataaata tctatgtgac
    25621 tcaaaaggta ctatttaaca tttctttaaa aaaaaggcac tgttagtgct tttttttagt
    25681 attcaaaaaa ctatttttgt aaataatact tatgaggtga tttatatgaa aaaaaagata
    25741 tttttattag taatactttt aattatcggc ttaacagctg tttatttcgg cagcggaatt
    25801 gacagatccc tgaaaagaat acctgaaagc gcagtcttac tgtaatacct ttaattatac
    25861 attaggaggt aaaatgaaat ataataaaga caactataaa caagtagttg atgagtttac
    25921 acctaaaaac aaaatgctta aaaactgtgt gatggcattt atttttgggg gagccatatg
    25981 tttggtggga gaaattatca atgactcttt tgttgcctgg ttaggtatat cagatgaaga
    26041 agcatcaccc atgacagctg tggtactaat aggtattagt gcacttttga cgggtttggg
    26101 ctggtatgat aaactaggca aaataggagg agccggtaca gtcgttccaa taaccggatt
    26161 tgcaaattcg gtagtttctc ctgccttgga atttaagaga gaaggattga ttttaggtac
    26221 ggctgcaaat atttttaagt tagcaggacc tgtcttggta ttcgggtact tttcttcatt
    26281 tatagcaggt ttgttaagta tgttatttaa gtaaggagga aaaatgaaaa agacaggaaa
    26341 aacttatata attaaagacg atgtagtaat aagagatact tacaccatag tcggcaaaaa
    26401 ggaaggacag ggacctttaa aagataaatt tgatatggta atagaagatg atttatgggg
    26461 agaagacaca tgggaaaaat gtgaattaaa aatgcagaaa attgcagtag aaaatttgaa
    26521 aaacaaaaat agtttaataa atgaaaacat tgacctttta atttcaggag atttgttaaa
    26581 tcaaatcaca tcttcttcat ttgctgcaag agccttggaa ctgccataca taggagtata
    26641 cggcgcctgc tccaccatga cattgacaat gggtttaggc gcaatgataa tagacggagg
    26701 atttgctgaa aatgtaattt gtgttacatc aagccatttt tgcagtgcag aaaggcagta
    26761 cagacttccg cttgaaatgg gaggacagcg ccatatgtca gcacagtgga cagtaaccgg
    26821 cgcagctgcg gtatggctct caaaaagcgg aaagggacca aaaataaaac atgttacctt
    26881 tggaaaaatc gttgatttag gtgtaaaaga tgcaaataat atgggagctg ccatggcacc
    26941 ggctgcttac gactcattaa atcagcatct tcaggataca ggcattgatt atgatttaat
    27001 tgttaccgga gatttgggaa cagtgggtaa aggcatagtt aataagatgt tcaatgaaga
    27061 taataagagt atcgaatcaa aatacgatga ctgcggtaca attatttttg atattaacaa
    27121 acaggatgta catgcgggag gaagcggctg cggatgttca gcagcagtat tcggctcctt
    27181 tttatacaag aaattattga ataatgaaat aaaaaaactt ctgtttacag ctacgggagc
    27241 acttcattcg cctactgcaa atcttcaggg tgaaagtata cccggaatcg ctcactgctt
    27301 gggtattgaa gtttaggagg gcatatgaat tatttgatga gttttgtaat aggcggtttg
    27361 atatgtgtag tcggccagat aataatcgat acattcaaaa agaataatgc ttatctgctt
    27421 gtgtttttcg ttgtgacagg tgcaatattg ggattcttcg gagtttatga taagcttgta
    27481 gaaatagggc attcgggagc taccgttcct cttctcggct ttggcaactc cttagccaag
    27541 ggagctatgg aagaagccgc aaataaaggg tttatgggag cattgagcgg aggagtgata
    27601 aaaacagcac ccggagttgc tgcagcattg ttattcggat atatcgttgc tgttatattc
    27661 aatcctcatg gtgataaata aaagagttca gaatcgaaat tctgaactct tttatattca
    27721 taattaccat tataaataat tgttattcca taaagtgtca ttctgagggc tttagcccga
    27781 agaatctcat gagatccttc gctttgctca ggatgacgta tgagatcctt cgctttgctc
    27841 aggataagcg accgacacca aatcaaagat ttgggtcgtc tgcttaattt tctatggatt
    27901 ccaatatttc atctatcggt ttatcagggt cttcttccga attctcaaat tcatcgatta
    27961 tctgatttat aatatcttcc aatgtatcac cattgccgtt attgccgttg ccgtttccat
    28021 tatggccatt gccgttatta ccgtttccat taccattatt accgtttccg tcgccttcat
    28081 tcaaccactg gtcataatgc catttggtgt gataataaca agtcctggtt ggaacctgat
    28141 actggtagtc ttcggtaata aatccggaag ggcctaactt ttcaacttca attcctaaaa
    28201 catgagttat ttgataattt tcatcaactg acatctgacc ggcatatatt tgctgaagtt
    28261 cttccatagt gaaaataatt ctgtcttcta aaatttgctg atataatttt tttgcttcat
    28321 agttatctgt cttttcttca gggtcaaaaa gcgggtctct tgtaaagaac actctttctt
    28381 ccaccaaatt acccggacag tatattgatt ttttcatatt tgagctggta catacttcag
    28441 ctttaacatg tacgtcgcaa gtttcagtag gttgagttcc gggtataaaa tattccgtaa
    28501 taatctgact tcctctctgg tcaagtgcac aaaggtcacc cggcagcttt cctgatactt
    28561 tacaaacact aattggtatt aggtttggat ttcgtgtaaa tgttgcagga ggcagacctt
    28621 gatgtatcgg agtcataatt gcactccaaa gtcttgcgtt gttgccgctg tctccggaaa
    28681 gcttcatttg aacattatca ttaccaaccc aaattcctcc tacatagtat ggtgaaaaac
    28741 cgacatacca gaaatctccg tttgcctgtg ttgtacctgt ttttcctgca acttcgatac
    28801 ccaattcctg gggaagtctt gccctatatg aaagtccggg atttactgtt gttcttaaaa
    28861 tatccttcat cagcgaagcg acttcaggac tgtatgccac atgtgtttca gggatatttt
    28921 ccagtattgt ttcaccctta gtattaacaa ctcgcgtata tactatcggc tctacatata
    28981 ctccatcatt ggcaatcgcg ccgtatgcag cagtcattgc caatggagaa aatcctttgg
    29041 taagtccgcc caaagcaaga gatgccaagt tcacgtcatt gtaataagga tcttcttgcg
    29101 gagatacaaa ggtatctttg tcagggttat tttggtcgat tagtcttaat cttgataatg
    29161 agtttatagc agcatcaagt ccgattgttt caagcatggt aacagcattt gtatttattg
    29221 actgctctat cgaccttcta agggtttgta taccccagta ttttatattt ctgtgttcat
    29281 accagttttt aggccatctt tcaccgcttt gattgtattt tgggaggtca tcaattgggt
    29341 aagcagctga atatcctaaa tcaagagccg ccaaatatac cgataacggt ttgattgtcg
    29401 agcccggctg tcttgtcgca tctacagcac gattaaaggt tttgcttcct tcaatctgcc
    29461 ttccgccaat cattgcttta atttttcctg ttttgtaatc gattattact gctgctgatt
    29521 gaggctgtac aatacctttt tcctggaaga agaaatatcc ttccggtatt tttaaaatac
    29581 cgtcactccc tatattgaac atttccttat ttttatccaa ataattttta ggtatcaaaa
    29641 aggtcccttt tgatcctttt ttctcaatta tttcatagtt atttcccaca ttgagggctc
    29701 ctatggtgtg agaaacaaag ttcattttat cgtcaattgt ataagcatca acgatatcta
    29761 tagttgaaga atatatatcg aattttttag agttaattac caagtttccg ttttcatcaa
    29821 tgctgtactc atcaggattc aagtaaatgt tgttttcttc atccattata ttttctttgg
    29881 caaagaatat aagctggccg ctttcattca ggatatttaa tcctgcatca agcattccgg
    29941 ttccttcccc tcctgaccat ctgaaatatc tccagtcctg ggcgataggt ttatctcctg
    30001 taggttcggc acccaaatat aatacaggga aattatcata agactgctct aaggattttt
    30061 gaatattaac atcaattgta gaagtaatgg ttaagccgcc tttatacaga tagttttctg
    30121 cttcctcata agacatgcct cgagtagcta ttaagtcttc cacaaccttt tctttcacat
    30181 agtccatagg tgttgatgaa ataccttcca tctttgtctg tcccggattt aatgcaaccc
    30241 gcatatcctc tgccatcgca gcatcatatt cttcttgggt aatgtattct aattcaagca
    30301 ttctgtttaa aatagtatgc tgcctgtcta ttgcgttttg attgtacaca caggcatatt
    30361 gaacagagcc gataaataca tttcctacta tatcttcttc gggaatatcg cttatatcct
    30421 ccaagttata acgattaaac ggtgcgtatt tcaccgtact ttttgcagcc gctgctaaca
    30481 atgcactttc agctaaggta agatcactta catcttttga aaaataagca tatgatgcag
    30541 cttgaacccc atattggctt tgccctagtg gtatggtgtt aaggtaattt tctagaattt
    30601 gttccttgga aagaactctt tccatcttaa tagccaaata aatttccgtt atttttcgct
    30661 cccaaacttt atcgctagta aggtataaat ttttaacaag ctgttgggta atcgtgcttg
    30721 caccttgtgc agtagggtct ccttctcgca cattatgtat aacagctccc ataatacgtc
    30781 ttatgtcaat accgggatgg tcttcaaaac gatgatcttc tattgcaata aaagcatctt
    30841 ttaagtattt aggtatttgt tttagcggta ctatttctct gttttcgttt gggtcgtgga
    30901 tttgctcaat aacatttccg ttttcatcta cgataactga gctttccgta agagtaccaa
    30961 gaacattcct tggatcgatt tcaggagcgt ttcttattat tcccatcacc attcccagaa
    31021 cggctccccc ggcaataatc actgccacaa gtattaaaag aagggttact ctgataataa
    31081 cctttctttt cttttttgcg gttctttttt tcttgtcatt tttatttata gaatctgaat
    31141 tattttcatt tatttcatta ttcttttttt cttttgacat aactacctcc gcgtttgcat
    31201 tagttagtat accacattta aagcaaatat tgtcaaagat tttcatctta ataattatat
    31261 aatagaaaaa gcttgaaagc aatagtttaa aaattactca ggaaaaatta tgaaaattgt
    31321 aagaaataaa gagtaattgc aaaaagacat ttgtaatgtt atattatata taatcccgca
    31381 gtatcattgc ttagggtata ttcaggatga cataaaggaa ttttttagat tgctatatat
    31441 tacggaggtt taatggaaaa agaaagatgt ttgctggttg cagttcaact gccgggcgaa
    31501 gaagaaaacg atatggaaga attggagcaa ttggttgaag ctgcaggcgg cgaagttatt
    31561 tcaagcattg ttcaaaacaa acaaagtatt gacaacagat tttatgcagg tcaggggaaa
    31621 attgaagaaa tagctaatta tgtgaaagaa ttagatattg atacagtaat tttcaatgat
    31681 gaattaagtg gttctcaaat acgaaatatt gaggagattg tcgaagcaaa ggtaatagac
    31741 aggactaacc tcatacttga tatatttgca aaaagagcct taaccaagga aggacagctt
    31801 caagtggaat tggcgcagtt aaaatacagc cttccgagac ttattggtgt aaataaaaac
    31861 ctgtctcgat taggcggagg aataggcaca agaggaccag gagagcaaaa gctggaaatt
    31921 gacagaagaa gaataaaaga aaaaatcacc gatatacaaa atcagttgga agtattggaa
    31981 aaggtaaggg aaaccaaaag aaagaaaaga atgaaggacc aggtgccgat agtttccatt
    32041 gtgggttata ccaatgcagg gaaatcaacc ttgctaaatg cactgatgga taatgaatac
    32101 agggaagaag gagaaaacaa gaaggttttc gcccatgaca tgctttttgc aacccttgat
    32161 acggagctta gaagagtgag attgcccggg ggaaggagtg ctgtcttttc cgatacagta
    32221 ggattcatta aaaagcttcc cacacaaatt gtggaggcct tcaagggaac attggaagaa
    32281 atcaaatatg ctgacttaat aattcatctt attgacataa atgatgaaaa tttgtcggct
    32341 cataaggaaa ctactattga gctgattgaa aaaattacgg gaagtgaaat acctattctt
    32401 accgtttata ataaggtgga taaaattttg aatgaaaaag tatctgtttt aaacaatggt
    32461 attatttaca tttcagcaaa atataaatat aatattcaag agctgctaga agctgtggat
    32521 aataagatta acggtccaaa aaacttttat gatttaaaaa taccgaatga taatataaaa
    32581 atttattact ggctttatga aaacagagtg actgaaaatg tggaatttga cggtgacggt
    32641 gtttcgctta gagttttatt gtacgaaaat gaaaaggata agtacaaaga ttttatcgga
    32701 gaaaaggatg agtaattata aatctatcca taaagaagga agagacgaaa taattattaa
    32761 taggtcaaga ttcattggta ctgcatgtcc cgttgaaaca gaagaagaag cattggaatt
    32821 tatagacaaa atcaagaaag aattcaagga tgcgactcat aacgtgtatg cttatgtaat
    32881 aggagaaaat tcaaacatac aaagattttc cgatgacggt gagccttcgg gaactgcagg
    32941 aatgcctgtt ttaaatgtaa taaagcagga aaacattaaa aatgtcgcag ttgttgtcac
    33001 aaggtatttt ggtggtgttc ttttaggagc gggaggactt gttagagctt atacaaaaag
    33061 ctcaaaaatt gctttggaaa gtggaaaaat agtagacaag aaattgtttt atgatgtatt
    33121 atttaagtta gactacacat tattagggaa aatggataat gaacttttga aaaacaatgt
    33181 tattatcaga gataaagaat atgaggaaca agtgctgttt aaactgatag tcgaaaagga
    33241 aaatttagac aaaatcaatg ccttggttgg agagatttca agcggtaaag ctgaaatttc
    33301 tgtgggacag gcaaaatact attccacaaa aaatggaaag atagttgatt gattattgtc
    33361 atcctgagcg aagcgaaggt cattgtcatc ctgagcgaag cgaaggatct cataagatga
    33421 taaaatgtca catcgcaatt aacagtaaag taaggaggaa aatatgtctg gacattcaaa
    33481 atggagtaat ataaaaaatc gcaaaggaaa acaagatgcg ttaagaggta aaatatttac
    33541 taagcttgca cgtgcaataa ctgttgcagc gagagaaggc ggagcagacc cggattataa
    33601 ttcatcattg gcaacagcaa ttgaaaaagc aaaggcacaa aatatgccca atgacaatat
    33661 agatagagca ataaaagcag gacttgcagc atcggcagga gaaaactttg aaacaatcac
    33721 ttatgaagga tacggaccga gcggtacggc atttatggtt cagtgcttaa cagacaataa
    33781 aaatagaact gcagcagaca taaggcatta tttttccaag cataacggaa acttaggaac
    33841 aacaggctgc gtaagctatt tgtttgataa aaaaggtgta atttccattg aatcagacgg
    33901 aattgatgaa gaagaactta tgatggcagc acttgaagca ggtgccgaag actttgaagc
    33961 ccaagatgac agctatgaaa taacaaccat acctgaagat tttatccaag ttctaaacag
    34021 tttaaaggaa gcaggctacg aaaatatcaa aggagatatc atgtatattc ctcaaactta
    34081 cgtgaaagtt gaagatgagg ctgcaataaa aaaccttgaa aaattaattg atgcattaga
    34141 agaaaatgat gacgttcaag aagtttacca caactgggat gagcctgagg aagaggaaga
    34201 ataagaatac taatttaacg aaattgcctt aaattttata tgcatgaaac gcttaataat
    34261 ttttaaggtt tttttatgca aactatggta caatatagat agtaaatata aaatataata
    34321 atatatagtt acacgattat aaaggagtga tgtaaagtgt atgtaccata cggtttattc
    34381 gatccgacaa tgctaatttt gattcctgcc attatattta ctttgtatgc tcaaagtaaa
    34441 gtgaaaacta attttgcaaa atatatgaga gttcaaacac gaagaggtta cacaggagca
    34501 gaagttgcca gaatgctttt aaaccaaaac ggcttgcatg atgtacctat agaaacagca
    34561 cggggtcaat taggagacca ttacgacccc actaaacgtg ttttaaggct atccggggaa
    34621 gtatatcaag gaagttccat agcatcagtc agtgtggctg cccatgaagt gggtcatgcc
    34681 atacagcatg caaacggcta tataccatta tccttaagga atatggtttt tcctgttgca
    34741 agatttggtt catctgcagc ttggatattt atattggttg gattaatgat tcccaacctt
    34801 gtaggtttaa tggatatagg tatcctattg tttggagctg ccgttttatt ccaaacaatt
    34861 actcttcccg tagagtttaa cgcaagcagc agggctttag aaatgctgga tgctaacgga
    34921 tatatctacg atgaagaaat aaaaggagcc aagaatgtac ttaatgctgc ggcattaact
    34981 tatgtggcgg ctatggcaag cggtttggca cagctcatga gattaat
//
LOCUS       DAOSHP010000012        33318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1039_length_33318_cov_8.541232, whole genome shotgun sequence.
ACCESSION   DAOSHP010000012 DAOSHP010000000
VERSION     DAOSHP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 33318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33318
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_33318_cov_8.541232"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      76..173
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            233..1129
                     /gene="metF"
                     /locus_tag="PLB33_02365"
     CDS             233..1129
                     /gene="metF"
                     /locus_tag="PLB33_02365"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOG62395.1"
                     /translation="MKIIDKFTEKKPLISFEIFPPKADYPIDTIFNAIDELYNLDPDF
                     ISVTYGAGGSTKDKTVEISSYIQNRYNLTSIAHVTCITSARKEINKILISLIENNVHN
                     VLALRGDYPKDCKISPPIDGFKNSMELIEFIKSNSNLCVGAACYPEKHPAARSLNDDI
                     DFLKRKMEAGADFLVTQMFFDNDLFYDFYDKIKKKNIDLPIFTGIMPILNKNQINTIL
                     NLTGNSIPNKFKMLLEKYENNPEALKEAGMSYAVSQIIDLLSWGVDGIHIYTMNKPST
                     ARKILENISAIRSVLTHESKFY"
     gene            1110..1799
                     /locus_tag="PLB33_02370"
     CDS             1110..1799
                     /locus_tag="PLB33_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOG62396.1"
                     /translation="MKVNFIKKDLVVINKDEALRYMGYNSKTVDIETQKLLDESIAEV
                     KDLAELKYVYKIFDIKKENNNISFENVINIKSNDLYTLFKNCEKSAVIAATLGFEVEK
                     RIRYYSLTNLSKALVFDACATSCIEALCDVAQAEIKEIALKEGFNTTHRYSPGYGDVP
                     ISHQSEILSALNAQKLIGLSVLDSYILIPRKSVTAFIGFIKDSKIYKKSCIDCNLFGN
                     CNFSKEGDRKC"
     gene            1793..4162
                     /locus_tag="PLB33_02375"
     CDS             1793..4162
                     /locus_tag="PLB33_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOG62397.1"
                     /translation="MLNKLKENFLFFDGAMGTMLQNSGLKTGELPEELNITNPEIIYN
                     IHKEYLNAGANIITTNTFGANKLKLINSSYTVDELITAGVKTARKAIEGFNEKHVALD
                     IGPIGTLLSPLGSLSFEEAYDLFKEQVTIGVNAGIDLILIETMTDIYELKAAILAAKE
                     NSILPVFATMSFQDKGRTFTGNDVKSFVFTAEGLGVDVLGVNCSLGPKQLQPIVDELL
                     KYSSIPIMVQANAGLPKYRNNTAYYDIDPSKYSHEINIMAKKGVAIFGGCCGTTPEYI
                     KSIVNALDGLKPIKIVKKNFTTAASSTKTVFLGDEVKIIGERINPTGKKRLKEALREN
                     NLNYIVREAIEQEDSGSDILDINVGLPGIDEKEMMKKVIKEVQSVVSLPLQIDSADYE
                     VIEAAARIYNGKPIINSVNGKKESMDKIFPIAKKYGALVVALTLDEEGIPATSEKRLE
                     IATNIIETAKVYGIPEENILIDPLVLTASAEQKAVMETLKAIPLIKNKYNVKIVLGTS
                     NVSFGLPKRNLLNTTFLSMALGFGLDAPITDSTNKSLMDVVRSFKVLANQDKDSKDYI
                     KTYTNISYENNIPSSETDLKEIIIKGLKDEAKKVSIKMLEQYNSLDIVSEFMIPALDI
                     VGEKYEKEEIFLPQLIRSAETSKIVFEVIKEKSIKEEITDEKKILLATVKGDIHDIGK
                     NIVKLILENYGYKVIDLGKDVDIENIVETVRKENITLVGLSALMTTTVKNMENTIKEL
                     KKEFPFIKIMVGGAVLTPDYALAINADYYSRDARGAVDIAKKVLEHKSM"
     gene            complement(4203..4439)
                     /locus_tag="PLB33_02380"
     CDS             complement(4203..4439)
                     /locus_tag="PLB33_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOG62398.1"
                     /translation="MTYTVLFFTHSGAIKFNRKCKKNNIQCELMPVPRALSSNCSISA
                     KIEYLDNIDNLIDDEIEKIFLTQNNINKLIFEAE"
     gene            complement(4439..4645)
                     /locus_tag="PLB33_02385"
     CDS             complement(4439..4645)
                     /locus_tag="PLB33_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOG62399.1"
                     /translation="MKKIDTCGMSCPQPVLMTKKALEESPEGLDIIVDNNTAKGNVER
                     FLKNSGYKVIITENEDNYILEARK"
     gene            complement(4658..5737)
                     /gene="yedE"
                     /locus_tag="PLB33_02390"
     CDS             complement(4658..5737)
                     /gene="yedE"
                     /locus_tag="PLB33_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOG62400.1"
                     /translation="MSDKKGIIIAGGAVGIISVLLVYFGNPANMGFCIACFLRDIAGG
                     LGLHRAEVVQYIRPEIIGLVLGAFLISVKNKEFQTKGGSSPFTRFMIGIAVMIGALIF
                     LGCPLRMVLRLAGGDLNALFGIAGFVAGIFVGVIFLNKGFNLKRSYKISKTEGYIFPV
                     LVLGLLILLIASPAFLFFSEKGPGSMAAPIIMSLTAGLIVGGLAQKTRLCMVGGTRDM
                     ILFKDMYLLSGFIAILVFSFIGNLAFGFFKLGFAEQPIAHTDGLWNFLGMAVVGWGSV
                     LLGGCPLRQLILSGEGNTDSVITVMGMLVGAAIAHNFGLASSAAGVTANGKVAVFICF
                     ALLGIVSFLNSESLVKAGNLSKASN"
     gene            6017..7162
                     /locus_tag="PLB33_02395"
     CDS             6017..7162
                     /locus_tag="PLB33_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG62401.1"
                     /translation="MRYVYMDNGATSFPKAPGVAESMADYILNVGTNVNRGAYSSSFK
                     AENIVYETRELLCELFRFNKPENVIFTKNITESMNVLIKGLLKEKDHVIVSSMEHNAV
                     MRPLNSLNKVEYTKVLCSKTGVLSIEDVENSIKPNTKAIIMTHASNVCGTILDLEKVG
                     QLCKKHNLFFIIDSAQTAGSIDIDINKLNADAIGFTGHKSLLGPQGIGGFIVNDGINN
                     EMTTLIEGGTGSLSAKEIQPDYMPDKYESGTLNIPGIYGLNASLKYLLNQGIKDIRDN
                     EINLINRFFEGLFNIKNIELIGKKTSTDRTGIVSIDFIHNDNGLVAHELSKDFGIMTR
                     SGLHCAPSAHKTLGTFPKGTVRFSLGHFTTIEEIDYAIECINKIANK"
     gene            7217..8113
                     /locus_tag="PLB33_02400"
     CDS             7217..8113
                     /locus_tag="PLB33_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOG62402.1"
                     /translation="MEFNQLESFISVVKNNSFSLAAKELYTTQPTVSNNIKNLEKELN
                     TTLLDRTSKIITLTDSGKLFYKYAVELINIRDKAKFSISEHDCRVEGEIEICASSIPE
                     EYILPYIIKDFLKIYPKVSFSITHKNSKDVIDNILGGKLNYGIVGAKYYSELLEYIDF
                     YEDELIFCAPYNKYPSVKNEYLDINTLLSEKFILRKTGSGTRRLIEQRLSEKGIDIDS
                     LNIISFIDSNEMIKKMIELGLGVSFISKIAVKNEIDLKLVKAFRIEDLNLKRSFYFVH
                     NKNRTLSPLVEAFKDFLTHWKY"
     gene            8133..9173
                     /gene="selD"
                     /locus_tag="PLB33_02405"
     CDS             8133..9173
                     /gene="selD"
                     /locus_tag="PLB33_02405"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:8181..8183,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOG62403.1"
                     /translation="MSSDEIKLTQMTKSAGUAAKIGPDVLAQVLCHLNYDKDENLLVG
                     MSTSDDASVYRISEDLALVQTLDFFTPVVDDPYVYGQIAAANSLSDVYAMGGEPKLAL
                     NITCFPLDLPKDVIKSILQGGHDKVKEANALVIGGHTVEDNEPKYGLSVTGFIHPKDV
                     VTNSNAKPGDCLILTKPLGLGILNTGIKAGLVSEEGYKTAVLTMTELNKFSKDAMMKV
                     GVNSCTDITGFGLLGHAYEMAVGSDVTIKFNSSKIPIIEEALSLARMGIIPAGAYKNM
                     EYIKEQVKISKTVVQEIIDCICDPQTSGGLLISVEKNKSQLLLDELKKSKTPYALIGE
                     VLEKQDYRIIVE"
     gene            complement(9214..10086)
                     /locus_tag="PLB33_02410"
     CDS             complement(9214..10086)
                     /locus_tag="PLB33_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62404.1"
                     /translation="MNKIITIVLVFMLMIVSSCYKFEENPENGEQNNNVNENIENTDE
                     SNENENNIKINADNNNIEKNDEGTDIESISENDDLEIEAEAVIHENNVRIVYPNGDIY
                     EGEMKNGMMDGFGIYTYVNGDVYEGDFKENKPHGHGKLTSPNNFVYEGGMKNGMMDGF
                     GIYTYSNGDVYEGEFKENYQHGHGRFTVPNKYVYEGEFRDDFINGHGVTIYANGDKYE
                     GEFVNGMKEGLGTYTYSNGNKFVGKYRDGKKEGSGVLYYIDGTVEEGYWENDIFVGSN
                     EQNNEINQIIEIEN"
     gene            complement(10261..11475)
                     /locus_tag="PLB33_02415"
     CDS             complement(10261..11475)
                     /locus_tag="PLB33_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG62405.1"
                     /translation="MDKKFNYGYVVAVLCFIIAFFYVGSAYNTGSLYVVPVTNHYGFA
                     RAEFSLIFSIISIFMIFASLSFVRLYRRLGIKNLVILGAILCVLAFFVYYKSTNLIQF
                     YIGAVLFGIGITYTNLMTFALLINSWFTENRGTILGLISAGSGFGGSVMSPVLGYLIL
                     TYGFKQAYLLTSIILFVLIIPIALFIKENPVGYATNSEEKLNIKKKTIGQLLKEQSVI
                     IGLTVIFLMGFSMVPWLHIFPSHLIDRGLNELFASKILGGVLFIMALSKILVGAVYDR
                     IGIKFAIGICLVSFVISSVLLLIVDSELIAWFFAALFGISLSSQTVLLPLFISAFAGD
                     EYLSDIIGIASALAGAGIAIGTPIINLTYDLTGSNDMILIAFTILGIFNMLLTIIALK
                     KAKNSKIQETDI"
     gene            complement(11516..12217)
                     /locus_tag="PLB33_02420"
     CDS             complement(11516..12217)
                     /locus_tag="PLB33_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOG62406.1"
                     /translation="MKYTIEKSVFELNPNIKFGILIGNNIRNSETRPEDEERLRRAEA
                     KFRNKVKPDEIRDLINVKLYREVMKNSGINPNKYLPSVEAMFKRIAKGGQLPVINALV
                     DLCNAVSIERGISLGGHDLKDIHDDLEVRYSRKGDVFLPFGAENYEDVEEKELIFTSG
                     NIVQTYKWLWRQSELGKLTLESTDVFFQLVGFDYEEGSSLYDAMSDIEYLVINRFHGT
                     CRKYLVDISNPSIEF"
     gene            complement(12288..13157)
                     /locus_tag="PLB33_02425"
     CDS             complement(12288..13157)
                     /locus_tag="PLB33_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOG62407.1"
                     /translation="MKRSDGYLLKDLKPFEKIIPYVMEKRSDAQNFSKQIFPAESIDK
                     YILEKKQSGYSFSYMHVFIAAYVRVIAERPQLNRFVMNNQIYQREKIYVSMAVKRSLR
                     DDGEETTVKFEFTGKETIFEIAEIIDKDIAKALSYSEDNNTDKLAERVMSMPGFVKKL
                     LVRTLKAMDKYNLLPKSIIDASPFHTTMFFTYLKSIDTQYIYHHLYDFGTTGLFAALG
                     KTVKIPIVENDQVVVKKCCEIGYTMDERICDGVYYARSFKLFEKYISNPRLLESELIQ
                     KVPKDQKVKKAFA"
     gene            13382..14716
                     /locus_tag="PLB33_02430"
     CDS             13382..14716
                     /locus_tag="PLB33_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG62408.1"
                     /translation="MDYINPLGTEKISKLLFKFSIPAITGMMINALYNVVDRIYIGNS
                     PDMGTDGIAGITVGFPIMIVFLALGVLFGVGGATNFSINLGRKNYTEAENYLGNTFSL
                     LILAGVLFAIIVRINLETILLFFGASEAILPYAMEYMKIIAFGAPFHIVSIGMNHFIR
                     ADGNPKLAMYTMFLGAGTNIILDPIFIYGFKMGMAGAALATIISQFFSFLWVTSYFIG
                     KHSRNKLKIEYLRLKSNLTGKITTLGLPGFSLQLATSALNAILNKNLVFYGGDIAVSG
                     MGVINSIQTLLIMPIIGLNQGVQPIISYNYGAKNYERVKDAAKLAIKAATIIVSFGFI
                     ITRLFPVQLISLFNRNTELIKFGKSAIFAWFLCFPVVGFQIVGSNFFQAIGRPKSAML
                     LTLTRQLILLTPAIIIFPRLWGVEGILYAAPFADFLSFLLTATFFYLGIRKL"
     gene            complement(14790..15974)
                     /locus_tag="PLB33_02435"
     CDS             complement(14790..15974)
                     /locus_tag="PLB33_02435"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG62409.1"
                     /translation="MISQEMIKLGTQRSVIRELFSYGLQRAEVVGAENVLDFSIGNPS
                     VPAPKEVQEAIIDIVKNNDPMVYHGYSTSQGHAGCRKAVAGNLNKRFNKSYTLNDIFM
                     TCGAAASLCITFRALTASADDEIIVLAPYFPEYKVFVEGQGAKMKLVQSKNDMRLNLE
                     GIKEAVNKNTKALLINSPNNPSGVVYSKEEIKELSDYLKKMQNELGQTIYLISDEPYR
                     ELVFNDEPVPFIPDYYDNTIICYSWSKSLSLPGERIGYVLVNDKCEDNKTVMAAVGGA
                     ARSLGYVCAPTLFQLVIEKCIDVKPNLEVYRKNRDILYDALTEMGYNVAKPDGAFYLF
                     IEAPNGDSKEFSDKAKEHDVLVVPGTGFGTDTHIRLSYCVDTEKCEKALIVFEKLIKE
                     IK"
     gene            complement(16053..16355)
                     /locus_tag="PLB33_02440"
     CDS             complement(16053..16355)
                     /locus_tag="PLB33_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62410.1"
                     /translation="MIIFLNLGSLIFGLIAWILPVINIFKYKKCEKWFVTSIISISLC
                     AVSIFFQLYYQNYLVKIDDLPALMDTISASSFLSAVLLFVTLILNAINLTIFLLKD"
     gene            complement(16355..16612)
                     /locus_tag="PLB33_02445"
     CDS             complement(16355..16612)
                     /locus_tag="PLB33_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62411.1"
                     /translation="MKKLKFILSDLLLLLGIEIFLSPFILYWFIHGDYERYIWIINGP
                     FPFNCFGGGPFQMLMYVSLFIIGAILIIVSLIIRRKHYGGV"
     gene            complement(16628..>17521)
                     /locus_tag="PLB33_02450"
     CDS             complement(16628..>17521)
                     /locus_tag="PLB33_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOG62412.1"
                     /translation="GTFAGFYLFSHLYSVFMKDDNSIANIISNKVYTAKKIKGDIIVF
                     NILLMSVLAVYTIFIVIQFKYLFSGGILPNGLNYSEYARRGFFELVVLSVLNIALILL
                     TTYLLRDKIYENKNKWALFTKLMLIYLCATTGILLASSYYRMSLYDSAYGFTRLRVLV
                     YIFLFFETVGLIATSVYIIKHNFNILAVYAAVGLTFYLTLNIIKIDEIIAKRNIDMYI
                     SGQAEEIDIDYLMNLSQDALPQIIRLSESDVQIMTRVKTIDFLKDMSAYYAQMENNWQ
                     SYNLTIEKNKDLMEASNYKWQ"
     assembly_gap    17522..17621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17622..17869)
                     /locus_tag="PLB33_02455"
     CDS             complement(<17622..17869)
                     /locus_tag="PLB33_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62413.1"
                     /translation="MEQKIKSKLSLYGIVSALTFIYLILTNNPGISAPLFFIIQFVIL
                     FFIVENNKGLVNIKGLFMLIPIFIISLNNFISANYMMMP"
     gene            complement(17853..18077)
                     /locus_tag="PLB33_02460"
     CDS             complement(17853..18077)
                     /locus_tag="PLB33_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG62414.1"
                     /translation="MAIIIQLDVMMAKRKIGLMELAEKINITPANLSILKNNKAKAIR
                     LSTLDEICKALNCQPGDILEYVEDEDGAEN"
     gene            complement(18088..18540)
                     /locus_tag="PLB33_02465"
     CDS             complement(18088..18540)
                     /locus_tag="PLB33_02465"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOG62415.1"
                     /translation="MYQKSIVHYMTKICIDILFIVAIICCILVPFSSSWLFQYFGIVG
                     ANTIFIKAILLASGISCAYILWQLKVIFKTLMNGNPFIHANVTCLRKIALACLVISFI
                     YLIKMIVMPTISTIIIIVIFIVACLLCLTLKDLFKQSIYYKDENDLTV"
     gene            complement(18741..19373)
                     /locus_tag="PLB33_02470"
     CDS             complement(18741..19373)
                     /locus_tag="PLB33_02470"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOG62416.1"
                     /translation="MLKIEQGLWDKGYNYIACIDEVGRGCLAGSVVTCAIIMPKGLLI
                     EGVNDSKKLTAKKREELFDIIIESSLGYGIGEYDCKKVDEYNIKNATKLAMVQAVENI
                     KDKEGNKIVPDYILVDAEKLDINIPQFNIIHGDAICHGIAAASIIAKVTRDRQMELLH
                     IQYPEYNFSKNKGYGTKEHIEALLEYGPSEIHRKSFVKKILDKNQQLSFL"
     gene            complement(19501..20343)
                     /gene="ylqF"
                     /locus_tag="PLB33_02475"
     CDS             complement(19501..20343)
                     /gene="ylqF"
                     /locus_tag="PLB33_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOG62417.1"
                     /translation="MNINWYPGHMKKTKELLLENIKLVNLIIEVVDARIPYSSKNPDF
                     DILFRDKKRLIVLNKYDLSDSNANKLWENYYRSKGWSVVLYNSTDNKELRKLNKAIAE
                     ATQEIVERYKKRGLLNKTIKAMIVGIPNVGKSTLINSLARKKSAKTGNMPGVTKGKQW
                     IHLAGNIDLLDTPGILWPKFDDEECALNLAFTGAIKDDVLVLEEIALKFVERMQQMGK
                     ENLILERYGIEAGENPLDILNNIAHKKGFLINKTDIDYHRVARLLFNDFRNGKLGNIT
                     LEMP"
     gene            complement(20423..20767)
                     /gene="rplS"
                     /locus_tag="PLB33_02480"
     CDS             complement(20423..20767)
                     /gene="rplS"
                     /locus_tag="PLB33_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOG62418.1"
                     /translation="MDLIKSIENEQVKGVTPFNVGDTIKVHVKVKEGNRERIQIFEGI
                     VIKKQGESNRATFTVRKISYGVGVERTFPVHSPKIEKIEVVRRGKVRRARLFYLRERT
                     GKATKVKELRRY"
     gene            complement(20866..21582)
                     /gene="trmD"
                     /locus_tag="PLB33_02485"
     CDS             complement(20866..21582)
                     /gene="trmD"
                     /locus_tag="PLB33_02485"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOG62419.1"
                     /translation="MKFEIVTLFPELIEVYKKNGIIGRAVENKLFEIEYVYLRDYSED
                     KHKKVDDYPYGGGPGMLLRPEPMFRALEAIKKHNSYIVYLSPKGSLFNQNKAKELSDK
                     EHIILIPGHYEGIDQRIIDKYVDEEISVGDYVLTSGELPALMIIDAVGRLIKGVLGSD
                     ESSKEESHSENLLEYPQYTRPESYMGLDVPEILLSGHHKNIEEWRLFKAIEITLKRRP
                     DMIKDKSIIEKYNELAKKYK"
     gene            complement(21579..22079)
                     /gene="rimM"
                     /locus_tag="PLB33_02490"
     CDS             complement(21579..22079)
                     /gene="rimM"
                     /locus_tag="PLB33_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227124.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOG62420.1"
                     /translation="MKDYIKVGKIINTHGIKGYIKCMPMTDDLERFEELEYVYTEKDN
                     IKRVIDDVWYQKGMVYLKLRNIDDMNTAESYKDTYISILEDQLRELPEDSYYLFDLNG
                     MDVYSSTGEYIGKISEIYQTGANDVYEVRNNKSSFLIPAIKDVVKSVNIQEKKMIIDV
                     IEGLLE"
     gene            complement(22096..22326)
                     /locus_tag="PLB33_02495"
     CDS             complement(22096..22326)
                     /locus_tag="PLB33_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOG62421.1"
                     /translation="MGELVEFIAKSLVDNPDMVEVNEVEGSQSLIIELKVAPDDMGKV
                     IGKQGRIAKAIRTVVKAAATKDNKRVIVEIIQ"
     gene            complement(22338..22601)
                     /gene="rpsP"
                     /locus_tag="PLB33_02500"
     CDS             complement(22338..22601)
                     /gene="rpsP"
                     /locus_tag="PLB33_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOG62422.1"
                     /translation="MSVKIRLKRMGSKKKPFYRIVVADSRSPRDGRFIEEIGYYNPVV
                     EPKVVKINDESAIKWLNNGAKPTDTVSMLFKNNGIYEKRDENK"
     gene            complement(22722..24071)
                     /gene="ffh"
                     /locus_tag="PLB33_02505"
     CDS             complement(22722..24071)
                     /gene="ffh"
                     /locus_tag="PLB33_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOG62423.1"
                     /translation="MFENLSDKLQNALQKLRGKGKLTEKDIDLAMKEIKMSLLEADVN
                     FKVVKAFINNVKERSLGVEVMESLTPAQQVVKIVHEELAKIMGQGESKLKFNSNDITY
                     FMMCGLQGAGKTTATGKLARRLKKDGRRPLLVACDIYRPAAIKQLMVVGESVGVPVFE
                     MGDKKSPVTIAQEAINHARKHGNDVVIIDTAGRLHIDDELMEELVEIKKTVKPDEILL
                     LLDAMTGQDAVKVAETFNSYLDITGVILTKVDGDARGGAALSIRTVVDKPIKFLSVGE
                     KMDQLETFHPDRMASRILGMGDVLSIIEKAQDAFDEKQAIELQKKLKTQDFNLEDFLT
                     QLQQMKNMGPLDEILEMIPGLGGKKKLKGLKVDEKELVYTEAIIQSMTKKERMNPSII
                     NGSRRKRIAAGSGTSVQRVNSLLKQYEEMKKMMKKFQAMGTGKKKGFGGMGGFKLPF"
     gene            complement(24074..24421)
                     /locus_tag="PLB33_02510"
     CDS             complement(24074..24421)
                     /locus_tag="PLB33_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOG62424.1"
                     /translation="MSEKDFIYSILYDYYGDLLKKNQATIIEMYYNLDYSLSEIAEEM
                     NISRQGVHDALKRAEKNLAEYERKIKLYDKYVKFSEAAENIIQLSSKIDDKKYKDIIE
                     KIKLEALRIINEG"
     gene            complement(24725..25642)
                     /gene="ftsY"
                     /locus_tag="PLB33_02515"
     CDS             complement(24725..25642)
                     /gene="ftsY"
                     /locus_tag="PLB33_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOG62425.1"
                     /translation="MNNEKGFFAKLKEGLSKTKKNLTEQIEGVIFQYKKIDDNFLDEL
                     EEILITSDMGVGTTMDIINYIKEEAKKRKLEDTLELKDVIKEYLINVLKENERPDVTD
                     KQRVILIVGVNGVGKTTSIGKIAYRLKQSGNKVIVAAADTFRAAAVEQLKEWCQRAGV
                     DIIASEQGSDPGAVVFDAIQAAKARKADILICDTAGRLHNKKNLMNELSKIFKILDKE
                     FSEAHIDVYLVIDATTGQNGIVQAKLFKEACNINGLILTKLDGTAKGGIAFPIVNELK
                     IPIKYIGVGEQIDDLQDFNAQDFVNAIFE"
     gene            complement(25735..29316)
                     /gene="smc"
                     /locus_tag="PLB33_02520"
     CDS             complement(25735..29316)
                     /gene="smc"
                     /locus_tag="PLB33_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOG62426.1"
                     /translation="MYLKKIYVNGFKSFAEKTEIEVEKGITAIVGPNGSGKSNISDAI
                     KWVLGEQSAKSLRGGKMDDVIFAGTAKRLPLGYAEVRLTFNNESGIIPLEYKEVSISR
                     KLYKTGESEYYINKQQCRLKDIKELFMDTGIGREGYSFIGQGRVEDILSPNSDVRRQV
                     FEEASGIVKYKTRKEESERKLEKTKNNLDRVEDIIYELKERIEPLYEQSIRAQKFIEI
                     RDNLKKYELNYLAHEYEKHCEQLKALKNQRDMSLEEKLRLQKRRDYYAEIIESQKEKI
                     NDLQNKINETEALRDSKNKALEKYKSLRQVHKEKKLLYSSNIDNIKNEIGELEKRNLE
                     LEEKINVLIQEKVSCEEIYKRDIEKFESLNSDIKEYKEEIDEIVQSIEQQKNELFELH
                     KDINKNNSNKNTIESLIINNGDRIKHLNQEVNSEEEERQKRIETINNLQEEQRITREL
                     LIKKKAVLKSILEELNNAEKNKERLNLHISKLNEEYNGTKSKINILTNMEAYYEGYYK
                     SIKTVMNNKDKEPVLNSILGTVADVIKTEKKYEKAVEIALGSAIQNIIVNTDKEAEEI
                     IEYLKKNKIGRVTFLPINMLTERSLNDFEREVLKEECVIDTADNLIQTNSKYEKVIKN
                     LLGRIIIADNLNNGLKISKKLGNTVKIVTLQGDVINAGGSVTGGHISSNQALLGRKRE
                     IDELKAHLDKLSEELKEKNDSLKLLNFKIKELNNSVEEIKSFINEKNNYYEMNNSKIK
                     MLSEQNEKTTAAINKYKEEIHFIQSEKQKYENDLRYLNAETEKLKLLVEQKEKSIEET
                     ILMNNSNKLKYDEYNNTISIQRDKVSNITQNIKLIEEKLNNIHMEIERNKNSIKYKEE
                     SLSSIEQEIEAAREKIIEFTECSDALEEEIKSLNESYIKEKETHKNYQNEIYDYQNKI
                     NESNKLITDILDEEMKLNVKIERESSKIEDISAKLWEDYEINYAMALKYKDDSISQTK
                     LYSEVNSCKKAIKELGNINLDSIEEYKEVRERYDFLTKQEKDLKDAIEQLNEVIKELE
                     IQMKEKFVEEFYNIRAKFSEVFKKLFNGGNGDIYLENEEDALNSNIEITVQPPGKKLS
                     KISLLSGGEKALTAIALLFSILKTKPTPFCILDEIESSLDDLNIFRFSQYLKEFSRET
                     QFIVITHRKGTMEYADTLYGATMEEKGVTKLVSMKLSDEQSIIGTHS"
     gene            complement(29307..30404)
                     /locus_tag="PLB33_02525"
     CDS             complement(29307..30404)
                     /locus_tag="PLB33_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG62427.1"
                     /translation="MNKKIKIIPIFVPHVGCPNNCVFCNQRRITGQKNFTVDGEYVHK
                     IVKRYIETIDGNTNTEIAFFGGSFTAIDTKIQEELLKAAKHYKDLKIISSIRCSTRPD
                     AINDEILMLQKKYGMDIIELGIQSLDDEVLKISNRGHNKKQSIDASRLIKEYGFILGH
                     QIMPGLPGSSRIKDIKTCIESIEMKPDIVRIYPTLVIKDTELFDMYEKGLYKPLSLDE
                     AVEISAYIYSLYSINDISVIRIGLQNTESINNDKDVMAGPFHPAFRQLVEERIYYYAL
                     GSKLCKVNTKELGESITIHAPDNLISSIAGQKRSNINKLKERFSIKQISFINKKDDII
                     EIYNKNKRLFSFKESEVFQNYLNMQQGDLCI"
     gene            complement(30397..31104)
                     /gene="rnc"
                     /locus_tag="PLB33_02530"
     CDS             complement(30397..31104)
                     /gene="rnc"
                     /locus_tag="PLB33_02530"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOG62428.1"
                     /translation="MITGTVYEFEKIIGYKFNNIKILEESLTHSSYSNEDKAYNKVNN
                     ERLEFLGDAVLSISVSRYIFDEFPDYPEGDLTKLRAQVVCEDTLSLVAANLNLGKYLL
                     LGKGEESSGGRERKSILADAVEAVIAAIYLDGGYKQAEKFVLNNLTEYIKLAVKGKII
                     TDFKSYLQEYYQSKSQSCKIRYVVTKEEGPDHDKVFHVNVMVNKNVAGKGVGKNKKIA
                     EQNAAKDALIQEGCLNE"
     gene            complement(31199..31435)
                     /gene="acpP"
                     /locus_tag="PLB33_02535"
     CDS             complement(31199..31435)
                     /gene="acpP"
                     /locus_tag="PLB33_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOG62429.1"
                     /translation="MNDVFERIRELVAEKVGVEPEDISMETSFTDDLEADSITLFELV
                     MAIEDEFDIEVDDESIEKIETVGDIVRYLEDIII"
     gene            complement(31432..32430)
                     /gene="plsX"
                     /locus_tag="PLB33_02540"
     CDS             complement(31432..32430)
                     /gene="plsX"
                     /locus_tag="PLB33_02540"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOG62430.1"
                     /translation="MKIAIDAMGGDNAPEAVIIGSIQARDEYNLSLVLCGKEIEIKRI
                     LEKNTSNFNNIEIINAEDIISNDDKPVLAIRRKKESSMVKALYAVKEGNADAIVSAGS
                     TGALLSGGTLLIGRIKGIERPAIGSLIPNMSGGFCLLIDSGANVDSKPEFLYDFAIMG
                     DIYLKRVMNIKSPRIALANIGVEKSKGNKLTVETYELLVNSQLKLNFIGNIEARDILK
                     GEADIVICDGFTGNMILKTMEGTVLEIMKGLKEEIMSSMKTKLGGMLIKPALKNFKRK
                     FDYSEHGGAPLLGVKAPVIKAHGSSDAKAIKNAIRQAKICGESNVNELIEESIMRR"
     gene            complement(32548..32727)
                     /gene="rpmF"
                     /locus_tag="PLB33_02545"
     CDS             complement(32548..32727)
                     /gene="rpmF"
                     /locus_tag="PLB33_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOG62431.1"
                     /translation="MAVPKRKTSKAKRDSRRAANAKLTVPNTVECPQCHEPKLPHRVC
                     ISCGYYDGKEVVAVE"
     gene            complement(32740..33234)
                     /locus_tag="PLB33_02550"
     CDS             complement(32740..33234)
                     /locus_tag="PLB33_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOG62432.1"
                     /translation="MLLKINKFLSSDNVSMTVNENFVIDDEEFLEETRLDKNILLSGE
                     IFKIDDSLTFSGRIEYTLTDECARCLKEFNNRINYKFQASLVHKEDKESDEVQMVITD
                     GIIKMDDIIKQLIYLSLPMKSLCDKDCRGICPNCGANLNNEKCQCVNSLTDPRFDKLK
                     DLLI"
BASE COUNT        10261 a         6213 c         4865 g        11879 t
ORIGIN      
        1 tgcttacaaa atatttaatt tatctattga caattaggtg ataataatta tatactttga
       61 tttaataatt taataaattc atcaagagtg acggagggac tggcccgatg aagtccggca
      121 accaatctat gtatatggtg ctaattccag cggtttacac cggcagatga ggatttttaa
      181 gacaatttct ttcccaatgt cgggagagat tttttattta ttgggaggat ttatgaaaat
      241 aattgataag tttacagaaa aaaaaccctt aatatcattt gagatattcc cgccaaaggc
      301 agattatccg atagatacaa tttttaatgc tatcgatgag ctttataatt tagacccgga
      361 ttttatcagt gttacttatg gtgcaggcgg aagcactaaa gataaaaccg ttgaaatatc
      421 atcgtatata caaaaccgtt acaatttaac ttcaatagct catgttactt gtattacttc
      481 tgcaagaaag gaaattaata agatattaat atcattaata gaaaataatg ttcataacgt
      541 cttggcttta agaggagatt acccaaaaga ctgtaagata tccccgccaa ttgacggctt
      601 taaaaattcg atggaactga tagaatttat aaaaagtaat tcaaatttat gtgttggagc
      661 cgcatgctat ccggaaaaac atcctgctgc aagaagttta aatgatgata ttgattttct
      721 taaaagaaaa atggaagcag gtgcagattt tttggtaaca caaatgtttt ttgacaatga
      781 cttgttttat gatttttatg ataaaataaa aaagaaaaat attgatttgc ctatatttac
      841 cggtattatg ccaatactta ataaaaacca aataaatacg atattaaatc ttacaggtaa
      901 ttcaatacct aataaattta aaatgctgct agagaaatat gaaaataacc ctgaagcttt
      961 aaaagaagca ggaatgtcat atgctgtcag tcaaataata gaccttttgt cttggggagt
     1021 tgatggcatt catatttata ctatgaataa accttccacc gcaagaaaaa tattagaaaa
     1081 tatttctgca atcaggagtg tactgacaca tgaaagtaaa ttttattaaa aaagatttag
     1141 ttgtaattaa taaagatgaa gctttgagat atatgggata caacagcaaa actgtggata
     1201 tagaaaccca aaaacttctt gatgaatcaa tagctgaagt aaaagatttg gcagaactta
     1261 aatatgtata caaaatattt gatatcaaaa aggaaaataa taatatatcg tttgaaaatg
     1321 tgataaatat caaatcaaat gacttataca ccctttttaa aaactgtgaa aaatcggctg
     1381 taatagctgc tacccttggt tttgaagttg aaaaaaggat taggtattac agtctgacta
     1441 atttatcaaa agctcttgta tttgatgcat gtgccacttc ctgtattgaa gcattgtgtg
     1501 acgtggctca agctgaaata aaagaaattg ctttaaagga aggatttaat actacacata
     1561 gatacagtcc cggctatgga gatgtgccta tttcccatca atctgaaata ctgtctgcct
     1621 taaatgccca aaagcttatc ggtttgtctg ttttagattc ttacatattg attccgagaa
     1681 aatcggtgac agcttttata ggttttatta aagacagtaa aatatataaa aaatcctgca
     1741 tagactgcaa tttatttgga aactgcaatt tttcaaagga aggagataga aagtgttaaa
     1801 taaattaaaa gagaatttct tgttttttga cggtgcaatg ggtaccatgc ttcaaaattc
     1861 cggattaaaa acaggtgaac tgccggaaga attaaatatt acaaatcccg aaattattta
     1921 taatattcat aaagaatatt taaatgcagg agctaatatt ataactacaa atacattcgg
     1981 tgcaaataag cttaaattaa taaattcttc atacacggtt gatgaattaa taactgcagg
     2041 tgtaaaaaca gctaggaaag ctatagaagg ttttaatgaa aaacatgttg ccttagatat
     2101 cggtcccata ggtaccctcc tgtccccatt aggaagtcta agttttgaag aagcttatga
     2161 tttatttaaa gagcaagtaa caataggagt caatgcaggt atagatttaa tacttattga
     2221 aacgatgacc gatatatatg agttaaaagc cgcaattctt gcagctaagg aaaattctat
     2281 actccctgtt tttgcaacaa tgtcttttca agataagggt cgaacattta cgggtaatga
     2341 tgtaaaatca tttgttttta cggctgaagg tttgggcgtc gatgttttgg gcgttaactg
     2401 ctccttaggg cccaaacagc ttcagccgat tgttgatgag ttgttaaaat acagctcaat
     2461 accaataatg gtacaggcaa atgcggggct gcctaaatat cgcaacaata ctgcttatta
     2521 tgatatcgac ccttcaaaat acagtcatga aataaatata atggctaaaa aaggtgttgc
     2581 aatatttgga ggatgttgcg gcactactcc tgaatatatt aaaagtattg ttaatgcatt
     2641 ggatggatta aaaccgataa aaatcgtaaa aaagaacttt acgactgctg cttcatcgac
     2701 caagacagta tttttagggg atgaagttaa aattatcggc gaaagaatta acccgacagg
     2761 taaaaagaga ttaaaagaag cccttagaga aaataacctg aactatattg taagagaagc
     2821 tatagaacaa gaggactcag gctctgatat attagatatc aatgtgggac ttcccggtat
     2881 tgacgaaaaa gagatgatga aaaaggtaat caaagaagtg caaagtgttg tttcattgcc
     2941 tcttcaaatt gatagtgctg attatgaagt tattgaagct gccgcaagaa tatataacgg
     3001 aaagcccata attaattctg taaacggcaa gaaagaaagt atggataaaa tatttccgat
     3061 tgcaaaaaaa tatggggcct tggtagtggc tttaacacta gatgaggaag gtattcctgc
     3121 aacttctgaa aaaagattgg aaatagcaac aaatattatt gaaacagcaa aagtatacgg
     3181 tattcctgaa gaaaatatat taatagaccc gctcgttctt acagcttctg ccgagcagaa
     3241 agccgtaatg gaaacactta aagcaatccc tttaattaaa aataaatata atgtaaaaat
     3301 agtattggga acaagcaatg tttcttttgg gctgcctaag agaaatttat taaatacaac
     3361 ttttttatct atggctttag gttttggttt ggatgcacct attacggatt caacaaataa
     3421 gtctttgatg gatgttgtaa ggtcctttaa ggtactggcc aatcaagata aggacagcaa
     3481 ggattatatt aagacataca caaatatatc ctatgaaaac aatattcctt catctgaaac
     3541 cgacttaaaa gaaattatta taaaaggtct aaaagatgaa gctaaaaaag tttctataaa
     3601 aatgttggag caatataact cgcttgatat agtaagtgaa ttcatgatac ctgccttgga
     3661 tattgtaggt gaaaaatatg aaaaagagga aatatttctg cctcagttaa taagaagcgc
     3721 cgaaacatcc aaaatagtat ttgaagtaat aaaggaaaaa tcaataaaag aagaaataac
     3781 cgacgagaaa aaaatactat tagcaacggt caagggagat attcacgata tagggaaaaa
     3841 tatagttaag cttatacttg aaaattacgg ttacaaagtt attgacttag gcaaggatgt
     3901 ggatatagaa aacattgttg aaaccgttag aaaagaaaat attactttgg tgggcttatc
     3961 tgccttaatg actacaaccg taaaaaatat ggaaaataca ataaaagaat taaaaaagga
     4021 attcccgttc ataaaaatta tggttggagg tgcagtatta actcccgatt atgctttggc
     4081 aataaatgct gactactatt ctagggatgc aagaggtgct gtagatatag caaaaaaagt
     4141 attagagcat aagagtatgt gacctttgtg ttatataatc acaaaggtca gtccccctct
     4201 ttctattcag cttcaaaaat aagcttgttt atattatttt gagttaaaaa tattttttct
     4261 atttcatcgt caattaaatt atcaatattg tcaagatact ctatttttgc acttatactg
     4321 caattagaac tcaaagctct aggcacgggc ataagctcgc attgaatatt gtttttctta
     4381 cattttctat taaatttaat ggctcctgaa tgcgtaaaaa acaaaacagt atatgtcatt
     4441 atttccttgc ctctaatatg taattatctt cattttctgt tatgattacc ttatagcctg
     4501 aattttttaa gaatctttcc acattccctt tagccgtatt gttatctact attatatcca
     4561 atccttcagg actttcttcc aatgcttttt ttgtcattaa tacaggctga ggacagctca
     4621 tgccgcatgt atctattttt ttcatataat cctcccctta attacttgct tttgataaat
     4681 tacctgcttt tactaagctt tctgaattta aaaatgaaac aatacctaat aatgcaaaac
     4741 atataaatac ggcaaccttt ccgttagcag ttactcctgc tgcacttgaa gctaatccga
     4801 aattgtgtgc tatagccgct ccaaccagca ttcccattac agtgataaca gagtctgtat
     4861 tgccttcgcc tgatagtatt aattgcctaa gcggacatcc tcctaataat accgagcccc
     4921 aacctactac tgccatacct aaaaagttcc ataggccgtc agtatgtgca ataggttgct
     4981 cagcaaatcc caatttgaaa aatccgaaag ctaaatttcc aataaaacta aataccagta
     5041 tagcaataaa tcccgataaa agatacatat ctttaaaaag tatcatatca cgagttccgc
     5101 cgaccatgca caatcttgtt ttttgagcta atccaccaac tatcaagcct gccgtcagcg
     5161 acattattat aggtgcagcc attgagccgg gacctttttc actaaagaac aaaaaggccg
     5221 gtgaagcaat aagaagtatt aaaagtccta atactagaac cgggaaaata tagccttcgg
     5281 tttttgatat tttatagctt ctcttaagat taaacccttt gtttaagaaa ataactccga
     5341 caaatattcc tgcaacaaag ccggcaattc cgaataaagc gtttaaatct cctcccgcaa
     5401 gccttaaaac cattcttagc gggcatccta aaaatattag tgcgccaatc ataactgcta
     5461 tgcctatcat aaatcttgta aacggtgatg aaccgccttt agtttggaat tctttatttt
     5521 taacggatat taaaaatgct cccagtacaa gacctataat ttcaggcctt atgtattgaa
     5581 cgacttctgc cctatgtaat cctaatcccc ctgctatatc tcttaaaaaa caagctatac
     5641 aaaagcccat atttgcagga ttcccaaaat atacaagtag tactgatatt attccgactg
     5701 ctccgcctgc tataatgatt ccttttttat cactcattag ctctcctcct attattaata
     5761 attaataaat taacaataag attatatgat ggaaaattgc ttaaaacaat atgtatagaa
     5821 ttttacaatg cttcgagaag caactattaa tattgctgat attaaaatag aaaaccttga
     5881 attataaaca ttttcaaaat ttatataata aagcattaat gcaacctatt aaaatcttta
     5941 attgaacaaa tatttaccat atgttaaaat aatattgttt agaataaaat tagaaaatat
     6001 attagaggac tatactatga gatatgtcta tatggacaat ggagccacct cttttcctaa
     6061 agcacccggt gttgcggaaa gtatggcaga ctatatatta aatgtgggaa ctaacgttaa
     6121 tcgaggagct tacagttcat cttttaaagc tgaaaacatt gtatacgaaa ccagggagct
     6181 cctatgtgaa ttgttccgtt ttaataagcc tgaaaatgta attttcacta aaaacataac
     6241 cgaaagcatg aatgtgctta tcaaaggatt attgaaagaa aaggaccatg taatagtttc
     6301 ttctatggag cacaatgctg ttatgagacc cttaaattca cttaataagg ttgaatatac
     6361 aaaagttttg tgcagtaaaa caggagtatt aagtatagaa gatgtggaaa actctattaa
     6421 accaaatact aaagccatta taatgaccca tgcttcaaat gtatgcggaa ctattttaga
     6481 tttagaaaaa gttggacaac tttgcaaaaa acataattta ttcttcataa tagacagcgc
     6541 tcaaacagca gggtctattg atatagatat aaataaacta aatgcggatg caattggatt
     6601 tacaggccac aaaagtttac tgggtcctca gggaataggc ggattcattg ttaatgacgg
     6661 aatcaacaat gaaatgacca ccttgattga aggcggcacg ggtagtctgt cggctaaaga
     6721 aatacagcct gattatatgc ctgataaata tgaatcagga actttaaata ttccgggcat
     6781 ttacggattg aatgcatcat tgaaatactt gttaaaccaa ggtattaaag atatcaggga
     6841 caatgaaatc aacctcataa acagattttt tgagggttta tttaatatta aaaatattga
     6901 attaatcggt aaaaaaacaa gcactgacag aacaggcata gtatcgattg attttataca
     6961 taatgataac ggtcttgttg cacatgaact tagcaaggat tttggaataa tgactcgaag
     7021 cggtttgcac tgtgccccat ccgctcataa aacattggga acattcccta aaggtacggt
     7081 tcgcttttca ttaggtcatt ttacaacaat agaagaaata gattatgcaa tagaatgtat
     7141 taataaaatt gccaataaat aattagaagc tgcaaggtga cagtaccccc gtccccttgt
     7201 cataaaggaa ggtagtatgg aattcaacca actggaaagt tttataagtg tagtaaagaa
     7261 taacagtttt tcattagctg ctaaagaact ttatacaaca caacctactg taagcaataa
     7321 tattaagaat ttagaaaaag aactgaatac aacccttttg gacagaacca gcaaaataat
     7381 taccttgacg gattccggaa agctctttta taaatatgcc gtcgagctta tcaacattag
     7441 ggacaaagct aaattttcta tctcagaaca tgactgcaga gttgaaggtg aaatagaaat
     7501 atgcgcaagc tcaataccgg aagaatatat tctaccttac ataattaaag actttctaaa
     7561 aatatatccc aaggtatctt tttcaataac ccacaaaaat tcaaaagatg taatagataa
     7621 tatattaggt ggaaaactaa actatggtat cgtaggagca aagtattatt cagaattatt
     7681 ggaatatata gatttttatg aagatgagct tattttttgc gcaccttaca ataaataccc
     7741 ttccgttaaa aatgaatact tggatattaa taccttgctt tcggaaaaat ttattttaag
     7801 aaaaacaggg tcgggtacca gaagattaat tgagcagaga ctttcagaaa aaggtattga
     7861 tatagatagt ttaaacatca tttctttcat tgacagcaat gaaatgatta aaaaaatgat
     7921 tgaactcgga ttgggtgtaa gttttatttc taagatagca gttaaaaacg aaattgattt
     7981 gaaactggtt aaagccttta gaatagaaga cttaaattta aaacgaagct tttattttgt
     8041 tcacaacaaa aatagaaccc tctccccttt agtagaagct tttaaggatt ttttaacgca
     8101 ttggaaatac taaaatacga ggaagtgaaa atatgtcttc agatgaaata aaattaactc
     8161 aaatgactaa aagtgccggg tgagcagcta aaattggacc tgatgtcctg gcacaagtat
     8221 tgtgccatct aaactatgat aaagatgaaa accttcttgt gggaatgagt acatcggatg
     8281 atgcttcggt ttacaggata agcgaggatc ttgccttggt gcagacattg gatttcttta
     8341 caccggttgt tgatgacccc tatgtttacg gccaaatagc agctgcaaat tcactgagcg
     8401 atgtatatgc aatgggcggc gagcccaagt tggctttaaa tatcacatgc tttcccctgg
     8461 acttacctaa ggatgttata aaaagtattc ttcaaggcgg gcatgataaa gtcaaggaag
     8521 ctaatgccct ggttatcggc ggccatacag tggaagataa tgagccgaaa tacggattat
     8581 ccgtaacggg atttatacat cccaaggatg tagttaccaa cagcaatgcc aagccgggag
     8641 attgtctgat tcttaccaag cctttgggat tggggatttt aaatacaggc ataaaggccg
     8701 gcttagtaag tgaagaagga tacaaaaccg ctgtccttac catgactgaa ttaaacaaat
     8761 tttctaagga tgctatgatg aaagtcggtg taaacagctg tacagacata accggattcg
     8821 gtttgctcgg tcatgcctat gaaatggctg tgggtagcga tgttaccatt aaatttaatt
     8881 cttctaaaat accgattatt gaagaagcct tatccttggc aagaatgggt attatccctg
     8941 ccggagctta taaaaatatg gaatatatta aggagcaagt taaaatttct aagacggtag
     9001 ttcaggaaat aatcgactgc atttgtgacc cgcaaacatc gggaggactt ttgatatcgg
     9061 tagaaaaaaa caagtctcaa ttacttcttg atgaacttaa aaaaagcaaa acaccctatg
     9121 cgttaatagg tgaagtatta gaaaaacaag attacaggat aattgtagaa taaactgcat
     9181 ataaaccggc tttatcgccg gtttttattt gttttaattt tctatttcta ttatttggtt
     9241 tatttcattg ttttgttcgt tactgccgac aaaaatatca ttttcccaat atccttcttc
     9301 aacagttcca tctatataat ataaaacccc tgatccttcc tttttcccat ccctatattt
     9361 cccgacaaac ttattcccgt ttgaataggt atatgttcct aacccctcct tcatgccgtt
     9421 aacaaattca ccttcatatt tatcgccgtt tgcatatatg gttactccat gaccgtttat
     9481 aaaatcatct ctaaattcac cttcatatac atatttattc ggcacggtaa atctgccgtg
     9541 tccatgctgg taattttctt taaactctcc ctcataaaca tccccgttag aatatgtata
     9601 aatgccgaat ccgtccatca ttccgttctt cattccgcct tcatatacaa aattatttgg
     9661 tgatgttaat tttccatgac catgaggttt attttcctta aaatctcctt cataaacatc
     9721 accgtttaca taggtataaa tgccgaatcc gtccatcatt ccattcttca tttcaccttc
     9781 gtaaatatcg ccgttcggat atactattct aacattattt tcgtgaatta ctgcttccgc
     9841 ttcaatttct aaatcatcat tttcactaat actttcaata tcagttcctt catcattttt
     9901 ttcaatattg ttattatcag catttatttt tatattgttt tcattttcgt tgctttcatc
     9961 agtgttttca atattctcgt ttacattgtt attttgctcc ccattctcag gattttcttc
    10021 aaatttatag catgatgaca ctatcattaa cataaaaact aatacaatgg taataatctt
    10081 attcataacc cgcctccaat accaacccct ttataatata tattattacc gtcaataatt
    10141 aaattaataa tagcactaat tctcattact gttaaattat gctagcgttt cattttcatc
    10201 aaaaaaaacc atgccttatg atgcatggta aagaatagtg ataaggattt tatactgaat
    10261 tcaaatgtct gtctcctgaa tcttggaatt ctttgctttt tttaatgcaa taatagtaag
    10321 cagcatgtta aaaataccaa ggattgtgaa tgcaattaaa atcatatcgt ttgaaccagt
    10381 taaatcatat gtaagattaa ttataggagt tccaatagct atacctgctc cagccaaagc
    10441 agatgctatc ccgataatat cacttaaata ttcatcccct gcaaaagctg aaatgaacaa
    10501 gggtaataac actgtttgag aacttaatga aatgccaaat aatgcagcaa aaaaccacgc
    10561 tattaactca ctgtcaacaa taagcaaaag aaccgagctt attacaaaag atactaagca
    10621 aataccgata gcaaacttaa ttccaattct gtcataaaca gcaccgacta aaatctttga
    10681 caatgccata ataaataaaa ctccgcctaa tattttagaa gcaaataatt catttaatcc
    10741 cctgtctatt aaatgagacg gaaaaatatg aagccatggc accattgaga aacccattaa
    10801 aaatataacg gtaagaccta ttattacact ttgctctttt aataactgcc cgattgtttt
    10861 tttcttaata tttaattttt cttcactatt tgttgcatat ccgacagggt tttctttgat
    10921 aaataaagca atcggaataa tcaaaacaaa caaaataatc gaagttaata aataagcttg
    10981 tttaaagccg taagtcaata taaggtaacc tagtacagga ctcataacag acccgccaaa
    11041 tccgcttcct gcagaaatca aacctaagat cgtccccctg ttttcagtga accagctatt
    11101 tataagcagg gcaaaggtca tcaaatttgt atacgttatt cctattccaa acaagacggc
    11161 gcctatataa aattgaataa ggttagtcga tttgtaataa acaaagaaag ccaaaacaca
    11221 taaaatggct cccaagataa ctaagttttt gatacctaag cgtctgtata atctcacaaa
    11281 agaaagactg gcaaaaatca tgaaaattga tataatactg aaaatcaacg agaattcagc
    11341 tctggcaaaa ccatagtgat tggttaccgg aacaacatac agactgcctg tattataagc
    11401 actcccaaca taaaaaaatg caataataaa gcataatact gctacaacat atccatagtt
    11461 aaattttttg tccataaaaa ctcctttgca aaaaaatgct gcctaaattg aagatttaaa
    11521 attctatgga tggattgctt atatctacaa gatatttcct gcaggtacca tgaaacctgt
    11581 taattactaa atattctata tcgctcatag cgtcataaag ggatgagcct tcttcataat
    11641 caaaccctac aagctggaaa aatacgtcag tgctttcaag agtaagcttt cctaattcgc
    11701 tttgcctcca aagccattta tatgtctgga ctatgttgcc gcttgtgaat atcagctcct
    11761 tttcttcaac atcctcatag ttttcagccc cgaaaggtaa aaacacatct ccctttctgc
    11821 tgtaacgcac ctctaaatcg tcatgaatat cttttaaatc atgcccgcct aaggatattc
    11881 ctctttctat ggatactgca ttgcataaat cgactaaggc atttataacc ggcagctgtc
    11941 cgccttttgc tattcttttg aacattgctt ctacggaagg aagatattta tttggattaa
    12001 tacctgaatt tttcataact tctctgtaca gcttcacatt tatcaaatcc ctaatttcat
    12061 caggcttaac tttatttctg aatttggctt ctgctcttct cagcctttct tcatcctcag
    12121 gccttgtctc tgaatttcta atgttattgc ctattaatat tccgaatttt atattaggat
    12181 ttaattcaaa cacagacttt tcaattgtat atttcatttt ttaactcctc ttagttattc
    12241 tgcctctcta ccatagagac tgctattttt ccacgtccgg attcttatta agcaaatgcc
    12301 tttttaactt tttggtcttt tggaactttt tgaataagct ctgattccaa taatctagga
    12361 ttgctgatat atttctcaaa gagcttaaag gaccttgcat aatacacacc gtcacatatt
    12421 ctttcatcca ttgtatagcc tatttcgcag cattttttaa caacaacctg gtcattctct
    12481 actatcggaa tcttcactgt cttgcctaat gcagcaaata agcctgttgt tccaaaatca
    12541 taaagatgat gatagatata ctgagtatct attgacttta agtaagtaaa aaacattgtg
    12601 gtatgaaagg ggctggcatc aataatactt tttggaagta agttatactt atccatcgct
    12661 tttagagttc taaccaataa tttcttcaca aacccgggca tcgacattac tctttcagca
    12721 agcttgtcgg tattattatc ttcactgtag gataatgcct tagcaatgtc tttatctata
    12781 atttcagcaa tttcaaaaat agtttctttt ccggtaaatt caaattttac ggttgtttct
    12841 tccccgtcat ctctaagtga acgttttaca gccattgaaa cataaatttt ttctctctga
    12901 taaatttgat tgttcattac aaaacggtta agctgcggtc tttctgcaat cactcttacg
    12961 taagcggcta tgaaaacatg catgtaacta aaactataac cgctttgctt tttttctaaa
    13021 atatatttat caatactttc cgccggaaaa atctgcttag aaaaattttg agcatcgctt
    13081 cttttttcca tgacataagg tattattttc tcgaaaggtt tcaagtcttt taataaatat
    13141 ccgtcacttc ttttcataca tcctccatat actaatctaa attctgcaat tcaaggagct
    13201 catataatac atagcggtgc gtaccggaaa aaaacatttt ccggaacacc gcttaatcga
    13261 agaataacac aaatttgcat tattatcaat tgttttattt tttattgctc aaatcttctt
    13321 gtttcaatat ataaaaattt atagtataat atcactatta ataacataaa cggaggcaaa
    13381 attggactac ataaatccat taggcacaga aaaaatatca aagcttttat ttaagttttc
    13441 aatacctgct attacgggca tgatgataaa cgcattatac aatgttgttg acagaatata
    13501 tataggcaac agccctgata tgggtactga cggaattgca ggaattacgg taggatttcc
    13561 cataatgata gtattcttgg ctctgggagt tttatttggg gttggaggtg ccacaaactt
    13621 ttcgataaat ttaggaagaa aaaattatac ggaagccgaa aattatttag gcaatacatt
    13681 ttcactgctt attttggcag gagtactctt cgcaataata gtgcgtatta acttagaaac
    13741 tatattgctt ttctttggtg ccagcgaagc aatccttcct tatgctatgg aatacatgaa
    13801 aataattgca ttcggagcac cctttcacat agtaagtatt ggtatgaacc attttatacg
    13861 agctgacgga aatccgaaat tagccatgta tactatgttt ttaggggcag gaactaatat
    13921 tattttagat ccaatattta tttatggttt taaaatggga atggcaggag cagctttggc
    13981 aactattatt tctcaatttt tctcattctt atgggttact tcatatttta tcggaaaaca
    14041 tagcagaaat aaattaaaaa ttgaatattt gaggcttaaa tcaaatttga caggtaaaat
    14101 tacaacctta ggtctgcccg ggttttctct tcaattggca acaagtgctt taaatgcaat
    14161 acttaataaa aatttagtat tttacggcgg tgatattgct gtttccggaa tgggagttat
    14221 aaacagcata caaactctgc tgataatgcc gattattggc ttaaaccaag gtgtccagcc
    14281 aattatcagc tataactacg gtgccaaaaa ttatgaaaga gtaaaggatg cagcaaagtt
    14341 agcaataaag gcagcgacaa taattgtttc ctttggattt attattacaa ggttgtttcc
    14401 tgtacaattg atatcattat ttaacaggaa tacggaatta ataaaatttg ggaaatctgc
    14461 aatatttgca tggtttttat gtttccctgt agtaggcttt caaatagtag gttcaaactt
    14521 ctttcaagct ataggcagac ctaaatctgc aatgttatta acccttacaa ggcagttgat
    14581 tttgttaacg cctgctatca taatattccc tagactctgg ggtgtggaag ggatactata
    14641 cgcagcacct ttcgcagatt tcttatcatt tttgttaaca gcaacattct tttatttagg
    14701 aataagaaaa ctataattaa aattgtaaat gacgatggaa gaagtagaaa tgaagaatgg
    14761 cgcatagcgc cattcttaat tatattatat tatttaatct ctttaattaa cttttcaaat
    14821 acaatcaatg ctttttcaca tttttcggta tccacacaat acgaaagtct tatatgtgta
    14881 tctgttccaa agccggttcc cggtactacc aagacatcat gttcttttgc tttatccgag
    14941 aattcttttg aatctccatt tggggcttct atgaataaat agaacgcacc atcaggcttg
    15001 gcaacattgt atcccatttc tgtcaatgca tcataaagaa tgtctctgtt tttcctgtat
    15061 acttccaaat ttggtttgac atcaatacat ttttcaataa caagctggaa aagtgtagga
    15121 gcacatacat aaccaagaga ccttgcagct cctcccacag cagccattac agttttgtta
    15181 tcctcacatt tatcgtttac taagacatac cctatacgct ctccgggcaa tgataatgat
    15241 tttgaccatg agtaacaaat aattgtattg tcataatagt ccggaataaa aggaaccggc
    15301 tcgtcattaa acaccaattc tctgtagggc tcgtcggaaa tcaaatagat tgtctgcccc
    15361 agttcatttt gcattttctt taaataatct gacaattcct ttatttcttc ttttgaatac
    15421 actactcctg aaggattgtt tggtgaattt ataagaaggg ccttagtatt tttattaaca
    15481 gcttctttaa ttccttctaa attaagtctc atgtcatttt tgctttgaac caatttcatc
    15541 tttgctccct gtccttcaac aaaaacttta tattcaggaa aataaggggc caagacaata
    15601 atttcatcat cagcacttgc tgtcaaagct ctgaaagtaa tacataatga agctgcagcg
    15661 ccgcaagtca taaatatatc attcaatgta taggatttgt taaatctctt gtttaaattt
    15721 ccggccactg ctttcctgca gccggcatgt ccttgcgatg tgctgtatcc gtgataaacc
    15781 attgggtcgt tattcttcac aatgtcaatg atggcttctt gcacttcttt gggagccggt
    15841 acacttgggt ttcctatact gaaatccaaa acgttttctg ctccgacaac ttctgctctc
    15901 tgcaaaccat acgaaaataa ttctctgata acagatcttt gcgtgcctaa tttaatcatt
    15961 tcttgtgata tcatattatc ctccaataat ttgtaatatt tcctttccac tatattacca
    16021 tattaataga taaaagtaaa tgttataaaa tatcagtcct tcagtagaaa tatagttaaa
    16081 ttaattgcat ttaatataag agttacaaaa agaagaactg ctgacaaaaa agaagaagcg
    16141 cttattgtat ccattaaggc aggcaaatca tcaattttta ctaaataatt ttgatagtaa
    16201 agttgaaaga atatagaaac agcgcataaa ctaatgctga taattgaagt tacaaaccac
    16261 ttttcacatt ttttatactt aaaaatatta ataacaggta gtatccaagc aattaagcca
    16321 aatattaaac tgcctaaatt aagaaaaatt atcattacac tcctccataa tgcttccttc
    16381 taataatcaa tgaaacaatt atcagtattg ctccaattat aaataatgaa acatacatta
    16441 acatttgaaa gggacctccc ccaaagcagt taaaagggaa aggtccgtta attatccata
    16501 tatacctttc ataatcgcca tgaataaacc aatacaaaat aaaggggcta agaaaaattt
    16561 caatacctaa taataataac aaatcagata gaataaactt caactttttc ataataattt
    16621 cactttgcta ctgccattta taattactag cttccattaa atctttgttc ttttcgattg
    16681 tcaagttgta gctctgccaa ttattttcca tttgagcata atacgcactc atatctttca
    16741 ggaaatctat tgttttaact cttgtcatta tttgaacatc actctcagag agccttataa
    16801 tttgcggtag tgcatcctgt gataaattca ttaaatagtc gatgtcaatt tcttctgcct
    16861 gaccgcttat atacatatct atatttcttt tggcaattat ttcatctatc tttataatat
    16921 ttaaagttaa ataaaatgta agcccaacag cagcataaac agccaatatg ttaaaattat
    16981 gctttataat ataaacagaa gttgctatca accctactgt ctcaaagaac aagaatatat
    17041 aaaccaatac tcttaatctg gtaaaaccgt aagcactgtc gtaaagagac atcctgtaat
    17101 aagaagatgc aagcaatatc ccggttgttg cacaaagata tataagcata agcttagtaa
    17161 ataatgccca tttattttta ttttcataaa ttttgtccct caataaatat gttgtcaaca
    17221 atattaaggc tatgttcaat acgctcaaaa ccacaagttc aaagaaaccg cgtcgggcat
    17281 attcggaata gttaagacca tttggaagaa ttccgccgga aaacaaatat ttaaattgta
    17341 taacaataaa aattgtataa acagctaata ctgacataag taatatatta aaaacaatta
    17401 tatcgccttt aattttcttg gcagtataaa ctttattact gataatattt gcaatgctgt
    17461 tatcatcttt cataaaaact gaatataaat gtgaaaacag gtagaatcct gcaaatgttc
    17521 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcatcatc atatagtttg
    17641 cactgatgaa gttgttcaaa gatattataa aaatcggaat cagcataaat aatcctttaa
    17701 tattgaccaa tcctttgtta ttctcaacaa taaaaaacaa aataacaaat tgaattataa
    17761 agaataaagg agcacttatt cccggattat ttgtaagtat caaataaata aatgttaaag
    17821 ctgaaacaat tccatataaa gataattttg atttaatttt ctgctccatc ttcatcctcc
    17881 acatattcaa gaatgtcccc gggctggcaa ttaagtgcct tgcatatttc atccaaggtt
    17941 gatagtctaa tagcctttgc cttgttattt tttaatatcg ataaattagc aggtgtaata
    18001 tttatttttt cagctaattc cataagtcct atttttcttt tagccatcat gacatcaagc
    18061 tgtataatta tagccataat acacccccta caccgttaaa tcattttcgt ctttataata
    18121 gatagattgc ttgaacaaat ctttcaaggt taaacacaag aggcatgcaa caataaatat
    18181 aacgattatt ataattgttg aaattgtagg cattactatc atttttatta aataaataaa
    18241 ggatataacc aaacaagcaa gagcaatttt cctaaggcag gtaacattgg catgaataaa
    18301 ggggttacca ttcattagag ttttaaagat aacttttaac tgccataaaa tatatgcaca
    18361 agaaatacct gaagctaata atattgcttt aatgaaaata gtatttgccc caactattcc
    18421 aaaatactga aacagccaag acgaagaaaa tggaaccaaa atacagcaaa taattgccac
    18481 tataaacaaa atatcaatgc aaattttagt catataatga actatgcttt tctggtacat
    18541 aaaatcctcc aaaatttatt attgtattct aattatagca gatatttttt gcatgtcaat
    18601 aaatatttat tgttttacga taatatttta ttgataaata aaaataaggc atcgtgactc
    18661 tcaatgcctt atatttctat ctgtatttct aattcattcc aataacttca tttttactta
    18721 aataaaaaac gtcgccgata ttataggaaa ctcagctgct ggttcttatc aagaattttt
    18781 ttaacaaatg attttctatg tatttcggaa ggaccgtatt caagcagagc ttcaatgtgc
    18841 tcttttgtac catacccctt atttttacta aaattatatt ccggatattg aatatgtaag
    18901 agctccattt gtcggtcacg ggttactttt gcaattattg atgctgctgc tattccgtga
    18961 caaattgcat ctccgtggat tatattaaac tgcggtatat ttatgtcaag cttttcggca
    19021 tcaacaagaa tataatcagg aactattttg tttccttctt tatcttttat gttttcgaca
    19081 gcttgtacca ttgcaagctt tgttgcattt ttaatattat attcatcaac ctttttacag
    19141 tcgtattctc caataccgta tcccaaagat gattctataa ttatatcaaa taattcttct
    19201 ctttttttag ccgtcagttt tttggaatca ttaacgcctt ctatcagtaa acccttgggc
    19261 ataataattg cacaggttac aacgctgcct gctaaacagc ctctccctac ttcatctatg
    19321 catgctatgt aattatatcc cttgtcccac agaccttgtt caattttaag catgttattc
    19381 tcctttaggg catctcgcag tctcattcac caaaatattt gttcgccttg ctcgcatatt
    19441 ttgggaattc gtgcgaatga cctaccatcc atttttctga atcctatcag aaaaatggtg
    19501 ttagggcatc tccaatgtta tgtttcctaa ttttccgttt ctgaagtcgt taaataataa
    19561 tcttgctact ctatggtagt ctatgtctgt tttatttatt aaaaagcctt ttttatgggc
    19621 tatattgttc aaaatatcca aagggttttc ccctgcttct atgccgtatc tttcaagaat
    19681 taaattttcc tttcccattt gctgcattct ttcgacaaac ttaagagcaa tttcctccaa
    19741 taccaataca tcatccttta ttgcacccgt gaatgcaaga tttaaagcac actcttcatc
    19801 atcaaatttc ggccacaata ttccgggagt atccaataaa tcaatattac cggccaagtg
    19861 tatccattgt ttgccctttg ttacaccggg catatttccc gtctttgcgc tcttctttct
    19921 tgccaatgaa tttattagtg ttgatttgcc tacatttggt atgccgacaa tcattgcttt
    19981 gattgttttg ttcaataatc cccttttttt atatctttca acaatttctt gagttgcttc
    20041 tgctattgcc ttattcagtt tccgaagctc tttattgtct gttgaattat aaagcacaac
    20101 cgaccagccc ttgcttctgt agtagttttc ccataattta ttcgcatttg agtctgataa
    20161 gtcgtattta tttaatacaa tcagtctttt tttgtctcta aacaatatgt cgaagtcagg
    20221 atttttactg ctgtatggaa ttcttgcatc tacaacttca atgattaaat taactaattt
    20281 aatattttcc agcaataact cctttgtctt tttcatatgt cccggatacc agtttatatt
    20341 cataatgtac ctctaatctg aaagggacac accttattaa aggaatgtcc cctttatatg
    20401 aggacgtccg ccctcaattc aattaatacc ttctcagttc tttaacttta gttgctttac
    20461 ctgttctttc tctcaagtag aacaaacgag cacgtcttac tttacctctt cttacaactt
    20521 caattttttc aatctttggt gaatgaacag ggaatgttct ttcaactcca acgccgtaag
    20581 atatttttct taccgtaaaa gtcgctctgt tgctttcgcc ttgtttttta attacgatgc
    20641 cttcaaatat ctgaattctt tctctgtttc cttctttgac ctttacatgt acttttatag
    20701 tatctccaac attaaaaggt gttacgcctt taacttgttc gttttctata gattttatta
    20761 agtccatttg tatacctccc ttcgccttaa cgttcttgtt acctgtacag aggaccgccg
    20821 tatactttta gcattatagc atatgaatca taatgcatac aacatttatt tatacttctt
    20881 tgctaactcg ttgtactttt caattatact tttgtccttt atcatatcgg gacgtctttt
    20941 taaagtaatt tctattgctt taaacaaacg ccattcttca atatttttgt gatgtcccga
    21001 taataatatt tcgggcacat ctaagcccat gtagctttcc ggtcttgtgt attggggata
    21061 ttcgagtaaa ttttcgctat gtgattcttc ctttgaagat tcatcagagc ccaatactcc
    21121 tttaatgagc cttcctaccg catcaataat cattaatgca ggcagttccc cgcttgttaa
    21181 gacatagtct cccacagata tttcctcatc aacatatttg tcgataattc tttggtctat
    21241 gccttcataa tgtccgggaa taagtattat atgctcctta tccgaaagct ctttagcttt
    21301 attctgatta aataaactgc cttttggtga taaataaaca atatatgaat tatgcttttt
    21361 aatagcttcc aaagctctaa acataggttc cggtctcaat agcataccgg gacctccgcc
    21421 ataaggataa tcatctactt ttttatgttt atcttcggaa taatccctaa ggtacacata
    21481 ttctatttca aacaatttgt tttctacagc tcttccgatt attccgtttt ttttataaac
    21541 ttcaattaac tccggaaata aagttactat ttcaaatttc attccaataa cccttcaata
    21601 acatcaatta tcattttttt ttcctgaata ttaactgatt tcacaacatc ctttattgca
    21661 ggtatcaaaa atgagctttt gttatttctt acttcatata catcgttggc tccggtttga
    21721 tagatttctg aaatttttcc gatatattca ccggtactag aatacacatc cattccattc
    21781 aaatcaaata aatagtagga atcttcggga agttctctca gctggtcctc aagtatggaa
    21841 atgtatgtgt ccttatatga ttctgcagta ttcatatcgt cgatatttct tagcttaaga
    21901 taaaccatac ctttttggta ccatacatcg tcaattacac gctttatgtt gtctttttcc
    21961 gtgtagacgt attctaactc ctcgaatctt tccaaatcat cggtcattgg catgcatttt
    22021 atatagcctt tgataccgtg tgtatttatt attttgccaa ctttaatata atccttcatt
    22081 tctacctcag aaaggttatt gaattatttc tactataacc cttttattat cctttgttgc
    22141 agctgcttta acaacagtcc ttattgcttt tgctattctg ccttgttttc ctattacttt
    22201 acccatatca tcaggagcta cttttaattc aattatcagt gattgactgc cttcaacttc
    22261 attgacttca accatatcgg gattgtcaac aagagatttt gctataaatt ctactaattc
    22321 acccatgata tcacctctta tttgttttca tctctttttt cgtaaatgcc gttatttttg
    22381 aaaagcatac ttactgtgtc ggtcggttta gctccgttat tcagccattt gattgcactt
    22441 tcatcgttta ttttcacaac cttcggctca actacaggat tgtagtatcc tatttcttca
    22501 atgaaacgac catctcttgg agagcgtgaa tctgctacta ctattctgta aaaaggtttc
    22561 tttttggaac ccattctttt taatctgatt tttactgaca ttatgtcacc tccttgattt
    22621 agtgtgtggg acacacctct ttttatggag ctatgctcct gtttgaggaa taccctgaat
    22681 atatatgaaa aaggaaaatc ctatttctaa caataatatg gttaaaatgg cagcttgaat
    22741 ccgcccatac cgccaaagcc tttcttcttg cctgtaccca tcgcttggaa ttttttcatc
    22801 atttttttca tttcttcata ctgctttaat aatgaattga ccctttgaac agatgttccg
    22861 cttcccgcag ctattctttt tcttctgctg ccgttgatta tcgaaggatt cattctttcc
    22921 ttcttagtca tagattgaat aattgcttcc gtatacacta attctttttc atcaacttta
    22981 agacctttta attttttctt gcctccaagt ccgggaatca tttccaatat ctcatccaaa
    23041 ggtcccatat ttttcatctg ctgaagctgt gttaagaaat cttctaaatt gaaatcttga
    23101 gtttttaatt tcttctgaag ttcaattgcc tgtttctcat caaaagcatc ctgggctttt
    23161 tcaataattg atagaacatc tcccatcccg aggattcttg atgccattct gtcaggatga
    23221 aatgtttcaa gctggtccat tttctctccc acagacaaaa attttatagg cttatccact
    23281 acggttctta tggaaagcgc tgctccgcct cttgcatcac catcaacctt tgtaagtatt
    23341 actcctgtaa tatccaaata agaattgaat gtttctgcaa ctttaactgc atcctgtcct
    23401 gtcattgcat ccaataacaa aagtatttca tcaggcttta cagttttttt aatttcaact
    23461 aattcttcca tcaactcatc atctatatga agccgtccgg cagtatctat aataacaacg
    23521 tcatttccat gttttctagc atgatttatt gcttcctgtg caattgtaac aggagatttt
    23581 ttatctccca tttcaaaaac aggaactcct acactttctc ccactaccat aagctgcttt
    23641 attgcagccg gtctgtatat gtcgcaagct acaagtaaag gtctcctgcc gtcttttttc
    23701 agtcttcttg caagctttcc ggtagctgtt gtttttcctg ccccttgaag cccgcacatc
    23761 ataaaatatg ttatatcatt cgaattgaat ttaagcttgc tttcaccctg acccattatt
    23821 ttggctaatt cttcatggac tatttttaca acctgttgtg cgggagtcaa actttccatt
    23881 acttcaacgc ctaatgacct ttctttcaca ttgttaataa aggctttaac aaccttaaaa
    23941 ttaacatctg cttcaagaag tgacattttg atttctttca ttgccaagtc tatatctttt
    24001 tcagtcagtt ttcccttgcc tctaagcttt tgtaatgcat tttgaagttt atcggataag
    24061 ttttcaaaca tttttaaccc tcattaatta ttcttaatgc ttcaagtttt attttttcaa
    24121 taatatcttt atatttttta tcatcaatct ttgatgacaa ttgtatgatg ttttctgctg
    24181 cttcgctgaa tttaacgtat ttatcatata gttttatttt ccgctcatac tctgcaaggt
    24241 ttttttcagc cctttttaaa gcatcatgta ctccctgcct gctgatgttc atttcttcag
    24301 caatttctga taagctgtaa tccaagttgt aatacatttc aattatggtt gcttgatttt
    24361 ttttaagcag atctccataa taatcataca agatgctata tataaaatcc ttctctgaca
    24421 taataacccc tgtaaagtat tttacttgac agtgaatttt accgcatcct gattattttg
    24481 tcaatagttt ttttgatata ttgagacata ttattcatta taatgaaaaa aataataaaa
    24541 atatcaagcg aacatttgca ggacacaatt ataattctta gcgaatgaag gcttaaaacc
    24601 cgttaagcag cttgcactgt ttgagcagag cgagtttgca agctgtaggg tttttagccg
    24661 tagtgagcgc atagaattat tcgtgtccga aaccgtgagc ggatattttt attatttttc
    24721 gtgtttactc aaaaattgca ttaacgaaat cttgagcgtt aaaatcctgc aggtcatcta
    24781 tttgctctcc gacaccgata tattttatcg gtattttcag ttcgtttaca ataggaaatg
    24841 ctatgcctcc ctttgctgtt ccatccaatt tcgttaaaat caaaccgtta atattgcatg
    24901 cttctttaaa cagctttgct tgaacaatac cattttgacc agttgttgca tctattacaa
    24961 gatacacatc aatatgggct tcgctgaatt ctttatcaag aattttaaaa atcttgctga
    25021 gctcattcat aaggttcttc ttgttatgaa gtcttcctgc agtatcacat atcaatatat
    25081 ctgcttttct tgcttttgct gcctgtattg catcaaaaac aactgctccg gggtctgagc
    25141 cttgctctga agcaattata tcaactcctg ctctttgaca ccactcttta agctgctcaa
    25201 ccgctgcagc tctaaatgta tctgcagcag caacgataac cttgttgccg gactgcttta
    25261 atctataggc aattttgccg atagaagttg ttttaccgac accatttacc ccaactatta
    25321 atattaccct ctgtttatct gttacatcag gcctttcatt ttccttaaga acatttatca
    25381 gatactcttt aattacatct ttaagctcca gcgtatcttc taattttctc ttttttgctt
    25441 cttctttgat ataattaata atgtccatcg ttgttccaac acccatgtcg gaagtaataa
    25501 gtatttcctc caattcgtca agaaaattat catctatttt tttgtattga aaaatcacgc
    25561 cttcaatttg ctctgtaagg tttttctttg ttttggaaag tccttctttt aatttggcaa
    25621 aaaagccttt ttcattgttc atatttgcct ccatcttatt tggggacatt cttctgtccc
    25681 tcttccgcta tctctttagc gactatccct atccgagtgg tttatcccca tagcttaact
    25741 gtgtgtccct ataatgcttt gttcatctga cagtttcatc gatactaatt ttgtaactcc
    25801 cttttcttcc attgttgctc cgtataaagt gtcagcatac tccatagttc ctttcctgtg
    25861 agtaattaca ataaattgtg tttcccttga aaattctttt agatattgcg aaaatctgaa
    25921 aatatttaaa tcgtcaagcg atgattcaat ttcatctaat atgcaaaatg gcgtcggctt
    25981 ggtttttaaa attgaaaaca agagagcaat tgcagtaagc gccttttctc cgcctgacag
    26041 caatgatatt ttgctcaatt ttttacccgg cggctgtact gtaatttcta tgttgctgtt
    26101 taacgcatct tcctcatttt ccaaataaat atctccattt cctccgttga aaagcttctt
    26161 gaatacttca gagaattttg ctcttatatt gtaaaattct tcaacaaact tttctttcat
    26221 ttgaatttca agctctttta ttacctcatt caactgctcg atggcatctt ttaaatcctt
    26281 ctcttgtttt gttaaaaaat catatctttc tctaacttct ttgtactctt ctatggagtc
    26341 caaattaata ttccccaatt ctttaattgc ctttttgcat gagttcactt cgctgtaaag
    26401 tttcgtttga ctaatgctgt catccttata ttttagtgcc attgcatagt ttatttcata
    26461 gtcttcccat agttttgcag aaatatcttc aatttttgaa ctttctcttt caattttaac
    26521 attcaatttc atttcttcat caagtatatc ggttatcagt ttatttgatt cattaatttt
    26581 attttggtag tcatatattt cattttgata atttttatga gtttcttttt cttttatata
    26641 gctttcgttt aatgatttta tttcttcttc caaagcatca gagcattccg taaattcaat
    26701 aattttttct cttgctgctt caatttcctg ctcaatagag gataagcttt cttctttata
    26761 ttttatggaa tttttgtttc tttctatttc catatgaata ttattcagtt tttcttctat
    26821 aagtttaata ttctgagtaa tattagatac cttgtccctt tgtattgaaa ttgtattatt
    26881 gtattcatca tatttcaatt tattggaatt attcattaat atggtttctt caatactctt
    26941 ttctttttgc tcgaccaata atttcagttt ttctgtttca gcattcaaat atcttaaatc
    27001 attttcatat ttttgttttt cactctgtat gaaatgaatt tcttctttat atttgttgat
    27061 agcagcagta gttttttcat tttgctctga cagcatttta atttttgaat tattcatctc
    27121 ataataatta tttttttcat taataaaact cttaatttct tctacggaat tgtttaattc
    27181 ttttatttta aaattcaaaa gcttcaatga atcatttttt tcttttaatt cttccgatag
    27241 tttgtctaaa tgagctttta attcatcaat ttctcttttc cttcccaaga gagcttggtt
    27301 tgagcttata tgccctcctg taacggaacc gcccgcattt attacatctc cttgaagtgt
    27361 tacaatttta actgtatttc caagcttctt ggatattttt agaccgttgt tcaggttgtc
    27421 tgcaataatt attcttccaa gcaaattttt aatgactttt tcatattttg aatttgtttg
    27481 gattaaattg tctgccgtgt caattacgca ttcttccttt agtacttctc tttcgaagtc
    27541 atttaaactt ctttctgtaa gcatattaat aggaagaaaa gtaactcttc cgattttatt
    27601 cttctttaaa tactctatta tttcttcggc ttctttatca gtgtttacga ttatgttttg
    27661 tattgcggag cccaatgcta tttcaacagc tttttcatat tttttttctg tttttattac
    27721 atcggccacc gttcccaata tactgtttaa tacaggctcc ttatccttgt tgttcattac
    27781 ggtttttatg cttttataat atccctcata atatgcttcc atatttgtca ggatatttat
    27841 ttttgatttt gtgccgttat actcttcatt taacttggaa atatgtaaat ttaacctttc
    27901 tttatttttt tctgcattat ttaattcttc caatatgctt ttcagtactg ctttcttttt
    27961 tatcagcagt tctcttgtaa tcctttgttc ttcctgcaga ttgtttattg tttctattct
    28021 cttttgtctt tcttcttctt ctgaatttac ttcttggttt aaatgcttaa ttctgtctcc
    28081 gttgtttatt attaatgatt caattgtgtt tttatttgaa ttgtttttat tgatatcctt
    28141 gtgcagttca aacaattcat ttttttgctg ctcaatcgat tgcacgattt catcaatttc
    28201 ttctttatat tctttaatat ctgagtttaa tgattcaaat ttttctatat ctctcttgta
    28261 tatctcttcg caagaaactt tttcttgaat taaaacatta atcttttctt ctaattccaa
    28321 gttccttttt tcaagttctc ctatttcatt tttaatattg tctatgtttg agctgtacaa
    28381 caattttttt tctttgtgta cttgtcttaa gctcttatat ttttctaaag ctttattttt
    28441 tgaatctctc agcgcttcag tttcattgat cttattctgt aagtcattaa ttttctcttt
    28501 ttgagattca ataatttcag cataataatc tcttcttttt tgcagcctaa gcttttcttc
    28561 caatgacata tctctttggt ttttcagggc tttaagctgc tcacaatgtt tttcgtattc
    28621 atgagctaaa taattaagct cgtatttttt tagattgtcc cttatttcaa taaatttttg
    28681 cgcccggata ctttgctcat aaagaggctc gatacgctcc ttaagctcat atattatatc
    28741 ttctactcta tccagattat tctttgtttt ttcaagtttc ctttcagatt cttcttttct
    28801 tgtcttgtat tttactattc cggaagcttc ctcaaaaact tgtcttctca catcagaatt
    28861 agggcttaaa atgtcttcca cccttccttg tccaataaaa gaatagcctt ctcttcctat
    28921 tccggtatcc ataaaaagct cttttatatc tttaagcctg cattgctgtt tgtttatgta
    28981 atattcgctt tcacctgtct tgtacagctt cctgctgatg cttacttctt tgtattcaag
    29041 tggtattata cccgattcat tgttgaaagt cagccttacc tcagcataac ccaggggcag
    29101 tctttttgca gttccggcaa atataacatc atccattttg cctcctctga gggattttgc
    29161 actttgctcc cccaaaaccc acttaatggc atcggatatg ttgcttttac cgctcccgtt
    29221 tggtcctaca atggcagtaa tacctttttc tacttctatt tcagtttttt ccgcaaagga
    29281 tttaaacccg tttacatata tcttctttaa atacataaat ccccctgttg catatttaaa
    29341 taattttgaa atacttctga ttccttaaaa ctgaataatc ttttattttt attatatatt
    29401 tcaattatat catctttttt atttataaag gatatttgtt ttattgaaaa tctttctttc
    29461 aatttattga tattagacct tttttggcca gcgatactgc ttatcagatt atcaggtgca
    29521 tgaattgtga tactttcccc aagctcctta gtattcactt tgcacagttt tgaccccaat
    29581 gcataatagt aaattctttc ttctaccaat tgcctgaaag cgggatgaaa gggccctgcc
    29641 ataacatctt tatcattatt gatggattca gtattttgaa gtcctatcct tataacactt
    29701 atatcgttga tactgtataa gctgtatata tatgctgata tttctactgc ttcatccaga
    29761 gatagaggtt tatataagcc tttttcatac atgtcaaaaa gctcagtatc ctttattacc
    29821 aatgtaggat aaattctcac aatatccggt ttcatttcga ttgactcaat acatgttttt
    29881 atgtccttta tcctgctgct tccgggaagt ccgggcatta tctggtgtcc taatataaat
    29941 ccatactcct ttatgaggcg gcttgcatct attgattgtt ttttattatg ccctcggttt
    30001 gaaatcttca aaacctcatc atcgagactt tgtattccta actcaattat atccattccg
    30061 tatttttttt gaagcatcag tatttcatcg tttatggcat caggtctcgt ggagcatctt
    30121 atactgctta tgatttttaa gtccttataa tgttttgcag cctttaataa ttcttcctga
    30181 attttcgtat cgattgccgt aaatgagccg ccgaaaaatg caatttccgt attggtatta
    30241 ccatcaattg tttctatata ccttttaaca attttatgaa catattctcc atcaacagta
    30301 aaattttttt gtcctgtaat tcttctttgg ttgcaaaata cacagttatt gggacaacct
    30361 acatgaggta caaaaatagg aataattttt attttcttat tcattcaaac agccttcttg
    30421 tataagagca tccttggcgg cattttgctc agctattttc ttgtttttac cgacgccttt
    30481 tcccgctaca tttttgttaa ccatcacatt cacatgaaaa actttatcgt ggtcaggccc
    30541 ttcttccttt gtaactacat atctgatttt gcatgactgg cttttacttt gatagtattc
    30601 ctgaagatac gatttaaaat ccgttataat ttttccttta acagcaagct taatatactc
    30661 agttaaatta ttcaatacga atttttcagc ctgcttgtat cctccgtcta aatatattgc
    30721 agcaataaca gcttccacag catctgccag tattgatttt ctttccctgc ctccggagga
    30781 ttcttcacct ttccctaata gtaagtattt ccctaagttt aaatttgccg caaccaagct
    30841 tagggtatct tcgcaaacaa cctgggctct caattttgtt aagtctcctt caggataatc
    30901 aggaaattca tcaaaaatat atctgcttac ggaaatgctt aagactgcat ctcccaaaaa
    30961 ttcaagtctt tcattattta ccttgttata tgccttatcc tcatttgaat atgagctgtg
    31021 tgtcaaggat tcttccaata tttttatgtt attaaattta taacctatta ttttttcaaa
    31081 ttcataaact gtacctgtaa tcaattttcc ctccggaata ataacatatc attgctactg
    31141 gatgacataa ctgatgtctg ctttcaaact atgggacgca ttgctgcgtc ccccttcttt
    31201 aaataattat atcttccaag tatctgacaa tatctcctac tgtctctatt ttttcaatac
    31261 tttcatcatc aacttcaatg tcgaattcgt cttcaattgc cataaccaat tcaaaaagtg
    31321 ttattgaatc ggcttctaaa tcgtctgtaa aggatgtttc catagaaatg tcttcaggct
    31381 caactccaac cttttcggct actaattccc taattctttc aaacacatca ttcacctcct
    31441 cataatgctt tcttcaatta attcatttac attgctttct ccgcaaattt ttgcctgacg
    31501 aattgcattt ttaattgctt ttgcatcaga gctgccgtgt gcttttatta cgggagcttt
    31561 aactcccaac agcggagccc cgccatgctc tgaataatca aattttcttt tgaagttctt
    31621 aagtgccggt ttaattaaca taccgcctag tttagttttc atgcttgaca taatttcttc
    31681 tttcaagccc ttcataattt ccagcaccgt accctccata gtttttaata tcatattccc
    31741 cgtaaacccg tcacatatta caatatcagc ttctccttta agtatatccc tggcttcaat
    31801 gttgccgatg aaattaagct taagctgtga atttacaagc agttcatatg tttcaacagt
    31861 cagtttgttt cccttggatt tttccactcc tatatttgct aaggcaatcc ttggggattt
    31921 gatattcata actcttttaa ggtaaatatc tcccataatg gcaaagtcat ataaaaattc
    31981 cggttttgaa tccacatttg caccggagtc tatcaataag caaaacccac ctgacatatt
    32041 gggtatcaat gagcctatcg cgggtctttc aattccttta atccttccta tcagaagggt
    32101 cccgccgctt aataaggctc ctgtgcttcc tgcagatact attgcatcag cgttgccttc
    32161 cttcaccgca tataaagcct tgaccatcga agattctttc ttgcgcctta ttgctaagac
    32221 aggtttgtca tcatttgata ttatatcctc tgcattaatg atttctatat tattgaagtt
    32281 tgatgtgttt ttttctagta ttctttttat ttcaatttct tttccgcata gtaccaagct
    32341 tagattatac tcatctctag cttgtatact tccgattata acagcctcag gtgcattgtc
    32401 tccacccatg gcatcaattg caattttcat attcacctct tatctaatat acttatatca
    32461 gtatatagca gtaatatttg ttttgcaata aaaaaatagt gccaagcact atttttttaa
    32521 atacccgaca cgggttttag agtcttatta ttcaacagct accacttctt taccgtcata
    32581 atatccgcat gatatacata ctctgtgagg tagctttggc tcatgacatt gtggacattc
    32641 cacagtatta ggaacagtta attttgcatt ggctgctctt cttgaatctc ttttggcttt
    32701 ggaagttttt ctcttaggta ctgccatttt tacacctcct tatatcaaca ggtctttcaa
    32761 cttatcaaat cgcgggtctg ttaagctgtt tacgcattga catttttcat tgtttaagtt
    32821 tgctccgcaa ttgggacata tgcctctaca gtctttatca cacagagatt tcatgggtaa
    32881 ggataaataa atcaattgtt tgattatatc atccattttt ataattccgt ctgttataac
    32941 catttgaact tcatcagatt ccttatcctc tttatgtaca agagatgctt ggaatttgta
    33001 attgattcta ttattaaact cctttaaaca tcttgcacat tcatctgtca gagtatattc
    33061 tattctgcct gaaaatgtca gactgtcgtc aattttgaat atttctccgc ttaataaaat
    33121 atttttgtcc agacgtgttt cttccaaaaa ttcctcatca tctattacaa aattctcgtt
    33181 tacagtcata gacacattat cagatgataa aaacttgttt atcttaagca acataatacc
    33241 acccttttta attcgcagag tttattatat gagaaaagaa aatatttgtc aaggtaatat
    33301 ttcttattat ttgcttgt
//
LOCUS       DAOSHP010000013        30830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1144_length_30830_cov_9.627360, whole genome shotgun sequence.
ACCESSION   DAOSHP010000013 DAOSHP010000000
VERSION     DAOSHP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 30830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30830
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1144_length_30830_cov_9.627360"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLB33_02555"
     CDS             complement(<1..1080)
                     /locus_tag="PLB33_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOG62433.1"
                     /translation="MLGNIISQIESYNPNADLGIVAKAYNFAEMAHSNAQTRFSGERY
                     FIHPYNVALILADLHMDVQTVCAGLLHDVVEDTGITFDEIKREFGEEIANMVDGVTKL
                     SKVKYRNKEERQAESLRKMIVAMSKDIRVVIIKLADRLHNIRTLQYMPKEKQYQKAME
                     TIEIYAPIANRLGIASIKWELEDLSLKYIDPEGYEDLLKKVQEKSEKRKDYISHIIDI
                     INKKLQESGMEADIKGRPKSLYSIYKKMYYKNRLFEQIYDLIAIRIIVENIKDCYGAL
                     GIVHTLWKPVPGRFKDYIAMPKPNMYQSLHTTVIGPEGEPFEIQIRTLEMHRTAEYGI
                     AAHWKYKEGIDDKTKYEEKLNWLRQM"
     gene            complement(1163..1681)
                     /locus_tag="PLB33_02560"
     CDS             complement(1163..1681)
                     /locus_tag="PLB33_02560"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOG62434.1"
                     /translation="MDLKEKIRVIEDFPAKGISFKDITTLLKDSEALHECVKEMAERF
                     KDVHVDIIAGPESRGFIFATPLAYLLKTGFVPVRKPGKLPAETIKYEYQLEYGTDSLE
                     IHKDAIKKGQKVLIIDDLLATGGTMYATAKLVEKLGGEVAGLGFLIELKDLKGREKLK
                     EYRVESLITYDK"
     gene            complement(1698..3611)
                     /gene="recJ"
                     /locus_tag="PLB33_02565"
     CDS             complement(1698..3611)
                     /gene="recJ"
                     /locus_tag="PLB33_02565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOG62435.1"
                     /translation="MKPVKLKVNEYNNDEIERISKRFNLSKTSAKILLNRNIKSFDDI
                     EQFLEPNFKYFERAENYKDLHKGCKRVIEAINNNDRILIYGDYDVDGVTSISQFVVFL
                     KKAGAYVNYYVPERESEGYGISSDFVNSVKSGEINFELLITVDCGIAEIEKIDEITKL
                     DKDVIIIDHHESREELPNAYAIINPKQKDCPSENKHLCAAGLSFKFLINLNKFLKIDN
                     IEDVLLEYACLGTIADIVDLVKDNRIIASQGLKKINKTKIIGLKKLIEVSGINGEIKS
                     YHIGFILAPRINASGRMDTAKKAIKLMLTKDEEEAEKLASELDSLNNARKEAENIIYK
                     EAIKKIESDFLYKNNVMVVYGDNWHEGVLGIVASRLTEKYYKPCVVISVKDGIGKGSA
                     RSVEHVDIFESFKHTDIYLEKYGGHKLAAGLTILEKNINSFTNELNNYIGSTTDDEYN
                     QIRADAILNISDINLRLYDEINRFEPFGSGNPKPLLALRDVSIKNIRKVGKEGKHISF
                     MLSDGDRHIPVIGFCKIGILEKILTMPQSYIVSLSENIYNGSRSLQLFLHDVEEHEEF
                     DYKIDVNKLKALEFLINKTKSKIIKTDLFILVEKLNKRYNTKITAEEIICMLKKADNI
                     QFALKNDILYIKK"
     gene            complement(3583..4128)
                     /locus_tag="PLB33_02570"
     CDS             complement(3583..4128)
                     /locus_tag="PLB33_02570"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HOG62436.1"
                     /translation="MQIGFIVILIIAIFVAIFAIQNGTPVPVDLFFARYEMPLAVIMM
                     ICLILGAVVVLILGTTRQFKKRSEYNELKNKLKTLEGEKAKHEIDMKALESEKAQFQT
                     NIEALETEKQRLQNEIADFSKKVTLLEDKIKLQDEELVAKNQEIQILKAQNIEKIEGD
                     TVITDEGAAQIDNETSEVESQ"
     gene            complement(4246..5580)
                     /gene="scfB"
                     /locus_tag="PLB33_02575"
     CDS             complement(4246..5580)
                     /gene="scfB"
                     /locus_tag="PLB33_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOG62437.1"
                     /translation="MIHVFNIKDLYFATDTSTGLVHAVDKIVFDLLMNEKFKDENKYS
                     ELYNEYGESTVREALSEIQYLIDNKMLYTEDIKYVNTIKPVIKAMCLNMTHDCNLRCE
                     YCFASQGTYNGEKAFLSFETGKKAFDYLVKNSGNRKNLEVDFFGGEPLMNFDVIKKLV
                     DYGRSLEKEYNKHFRFTVTTNGVLLDEEKMDYINENMDNVVLSIDGRKETNDRMRKTI
                     NKKGSYDLIVDNYKKFISKRGSKDYFARGTYTSNNLDFSEDVKHMRELGFDKISVEPV
                     VAKDEEKYALKKEHVDILKKEYEKLAEYYIESYKSKDRRFQFFHFNIELEGGPCIYKR
                     SIGCGAGTEYVAVTPSGDLYPCHQFVGNEEFIIGNVEEGITNRALADKFKNVSVNDKP
                     ACRDCWAKYFCSGGCHANAYNFNKDFTVPYDVGCELEKKRIECAIYIKAKIN"
     gene            complement(5721..5861)
                     /gene="scfA"
                     /locus_tag="PLB33_02580"
     CDS             complement(5721..5861)
                     /gene="scfA"
                     /locus_tag="PLB33_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOG62438.1"
                     /translation="MKYIKTLNNYKLTDNLSAKGCGECQTSCQSACKTSCTVGNQKCE
                     KE"
     gene            complement(5939..6307)
                     /locus_tag="PLB33_02585"
     CDS             complement(5939..6307)
                     /locus_tag="PLB33_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOG62439.1"
                     /translation="MKLGKLFKYSLFLFILSLIIYFILTVYIYYFSNNPGFEIVYSFI
                     VPICIFAVSMLYSRSVHEKGLLRGIEIWIVYFAMVLLIKVLFRYPSEIRILYNGVILI
                     MSILGGIIGVNMKNKSIKNK"
     gene            6578..9259
                     /locus_tag="PLB33_02590"
     CDS             6578..9259
                     /locus_tag="PLB33_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOG62440.1"
                     /translation="MYYRKSITETCEELNTAQSTGLTQEEAKKRLLSDGPNALVEKKA
                     KTKLQMFLAQLKDTLIYILFAAAAISIALNEITDAIIILLVVLLNAVIGMVQESKAEA
                     ALEALKNLSSPYAMVRREGRVMEIPASELVVGDLVILEAGRIIPADLRLIQSVNLKIE
                     ESALTGESVPVDKNADMVADKEVGIGDRLNMAFSSTSVSYGRGEGVVVYTGMDTEIGK
                     IAAMISESTEELTPLQKRLNDLGKVLGIVAVVIVVAMFGIAVLQGRDIIEMFITAIAL
                     AVAAVPEGLTAVVTIVLALGVTKMVRVNTIVRKLPAVETLGAVSIVCSDKTGTLTQNK
                     MTVTKVYLDGEIKNTDDLSYENNKIFLDGFILCNDASIANNTRIGDPTELALLDMGDK
                     INLSREGLEKTNPRINEQSFDSARKLMTTVHRDQDGKVMSYTKGAMDILLNRCNKIYI
                     NGETKDITNEHIEEINRAATEMAKGALRVLALGIKEDDDSASEEDLTFVGLVGMIDPP
                     RPEAKDSVKTLRSAGIRTIMITGDHKDTALAIAKELGIAEDESQCITGSELNEMTQEQ
                     LNEKVCDIRVFARVSPEHKVMIVKAFKSNGSIVSMTGDGVNDAPSLKSADIGVAMGIT
                     GTDVAKGASDMVLTDDNFATIEKAVAEGRGIYNNIKKTVLFLLSSNFGEVISMFSSIA
                     AGLIAPLQSIHILWVNLITDSLPALALGVDPKSKEIMNEKPRDPNESLFAKGGLIFTV
                     YNGIMIASLTLAAFLWSPVIHLNEAGIPVTLGNIRDLFNGVLSIPGVDLPDIIRHAQT
                     YAFTTLGVSQLFHAVGMRNYNKSLFQMSHTDNLAMIGAFSLGLLLQILVTEVHFLTEV
                     FETSQLSLKEWANLILVSAVPLLSHEVIVMGKKIFKK"
     gene            complement(9366..9710)
                     /gene="yajC"
                     /locus_tag="PLB33_02595"
     CDS             complement(9366..9710)
                     /gene="yajC"
                     /locus_tag="PLB33_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOG62441.1"
                     /translation="MPQEYSGILMLVVFFAIMYFTIIRPQKKREKETKAMRDSLATGD
                     EVITIGGIHGKVVKINDEIVTLEMPFGKQRIEFSKWAIGSVTKKGKEKAAKEEVVKEE
                     IEETVDDKEDDK"
     gene            complement(9731..10840)
                     /gene="tgt"
                     /locus_tag="PLB33_02600"
     CDS             complement(9731..10840)
                     /gene="tgt"
                     /locus_tag="PLB33_02600"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOG62442.1"
                     /translation="MFKFELIKESKECKGRLGRIYTPHGIIETPVFMPVGTKGTVKAM
                     TPEELKQLRAEIILGNTYHLYLRPGHELIREAGGLHKFMNWDGPILTDSGGFQVFSLE
                     NLRKIREEGVEFRSHIDGSKHFISPEVSMEIQNYLGSDIMMAFDECAPYPADYEYVKK
                     SMERTSRWAKRCKDYHKNTKSQALFGIVQGGMYKDLRIESAKQITDIEFPGYALGGLS
                     VGEPRDLMCEVMDYTVELLPKDKPRYLMGVGSPDYLFEAVERGIDMADCVLPTRMARH
                     GAFLTSKGQLTIKNAKYKNDFSPIDEECSCYTCRNYSRAYIRHLFGEKEILGARLASI
                     HNLFFLINLMKGIRISIAEDNFMKFKREFYENYGY"
     gene            complement(10848..11882)
                     /gene="queA"
                     /locus_tag="PLB33_02605"
     CDS             complement(10848..11882)
                     /gene="queA"
                     /locus_tag="PLB33_02605"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOG62443.1"
                     /translation="MTNFKTSDFYYELPERLIAQTPIKERDHSRLLVLDKKSGKIEHK
                     HFFNLVEYLNAGDCLVINDTKVLPARLFGNKKDTQTLIEILLLKRIDDKKWEILVKPG
                     KKARTGTEIVFDEKLLTGKIIDILEEGNRIIEFEYEGIFEEILDRLGEMPLPPYIHEK
                     LKDKDRYQTIYAKHLGSAAAPTAGLHFNDKLLEEIENKDVNIARLTLHVGLGTFRPVK
                     TEYINEHIMHSEYYELNESEANKINLAKSSGHKVITVGTTSVRTLETVADDEGIVNKS
                     KGWTNIFIYPGYKFKVVDSLVTNFHLPESTLIMLVSALAGKENIMKAYREAVEKEYRF
                     FSFGDAMFIK"
     gene            complement(11879..13561)
                     /locus_tag="PLB33_02610"
     CDS             complement(11879..13561)
                     /locus_tag="PLB33_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOG62444.1"
                     /translation="MKKNLIRLFIITALLTFLFPTTAFGKNKDDIYVRVRIRYPRLFN
                     EQVSFEGFDEIVLYDDEDELFCLNNSLTLRIDTYYSSNYYLSDKSQGTYGPYHAVLQD
                     EIYSSYEEAYLIAEELNEEFKVEFYPYLIEDGYIIAGGNFADKSSSQDLAEELNDEGY
                     IAESMNGNLENIIAYNKKNIPVFMYKKDMPVYFSSYNQDEEYEMIKVDNRAYRGRIRL
                     NIIEDFKLLTINHVELENYLYGVVPNEIPSSWGIEALKAQALAARTYAVYNIKPRQIY
                     DMEDNQNSQVYMGYDYEKTSTNRAVDETRGEMIYYDGELIQAFYHSTSGGSTENSENV
                     WYEKLPYLRGVNDEFSNNSGSPHTTWQTTYYKEDIIRNLNLDGNDVDELYGIQIKKVS
                     ENNRVVECIFLTDAGEISYKKENARLLLGLKSSWFSIVNGNIFCFITEYFSLDENRKD
                     ETPPSRGVLDLITEEETEETRNEIEFETGSIAGKHAITDDGIKKINREKLSFISSNGV
                     TTVDANSEQYNFEGRGWGHGIGMSQYGAKQMAEEGYTYEEIIKHYYTGVTVK"
     gene            complement(13542..14597)
                     /gene="ruvB"
                     /locus_tag="PLB33_02615"
     CDS             complement(13542..14597)
                     /gene="ruvB"
                     /locus_tag="PLB33_02615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOG62445.1"
                     /translation="MFERENEIVARTFNSCEEELELSLRPQKLPQFIGQNKVKEVLNI
                     FIESAKIRNQPLDHVLLSGPPGLGKTTLANIISNELGVNIRVTSGPAIERAGDLAAIL
                     TNLNENDVLFIDEIHRLNKTVEEIMYPALEDFALDIIIGKGPSARSIRLDLPKFTLIG
                     ATTRSGMLASPLRDRFGVMCNLDFYEPEDLKEIVLRSASIMNIEVEEKGAEEIAKRSR
                     GTPRIANRLLKRVRDYAIVKGEGIIDLKTADEALKMLEIDEIGLDKLDRRILNTIIDF
                     YKGGPVGIDTLAASIGEEKGTIEDVYEPYLLQIGYLNRTPRGRVVSESGYRHVKKIRK
                     IQDQINILEDDYEEEFD"
     gene            complement(14599..15183)
                     /gene="ruvA"
                     /locus_tag="PLB33_02620"
     CDS             complement(14599..15183)
                     /gene="ruvA"
                     /locus_tag="PLB33_02620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOG62446.1"
                     /translation="MISYIKGEVVKKGIDYLILDNNNIGYYINTSFSTLEKISEKEKI
                     IILTYMHIREDIIALYGFLTSDEIDLFKKLISVNGIGPRAGLSVLSTYEVNTVKEIIL
                     KEDVSGLSKVPGIGKKTASKIILELKDKVGTVEELKGSEIVVSSEMSDVIEALTSLGF
                     NYAEVKRTLGNMELSGKSENDIIKEALKNMNRQG"
     gene            complement(15192..15692)
                     /gene="ruvC"
                     /locus_tag="PLB33_02625"
     CDS             complement(15192..15692)
                     /gene="ruvC"
                     /locus_tag="PLB33_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOG62447.1"
                     /translation="MLILGIDPGLAISGYGILNYSGNKFKVIEYGCIFTESCEEFPKR
                     LKHIYESYISIIDMYKPEAVAIEELFYNKNAKTAMAIAQARGVHLLAAENKNVPLYEY
                     TPLQIKQGIVGYGRAEKKQVQEMVRVILKLNEIPKPDDAADGLAAAICHAHSLKFAKE
                     FKINKY"
     gene            15861..17012
                     /gene="rodA"
                     /locus_tag="PLB33_02630"
     CDS             15861..17012
                     /gene="rodA"
                     /locus_tag="PLB33_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOG62448.1"
                     /translation="MFKKESKLKRFDFILLITTILLCIYGFIAINSATMSKATGSEPF
                     LKTQIIAFGLGMVALFVLIFIDYDIYGSLYIPIYVMTNILLLYTLINPVEASEWGDVR
                     SWIAIGPIVFQPSEIAKFGIIISLSKFIDIHKDNINQPVVLLKIILFAFLPIALILMQ
                     PDLGTAVVFIFFVAIMIYISGIDRKYVLSVMIIGLILIIAGLSIFFQIMEDYTLNEDY
                     RLDRIVTFFYPELDPDDTGYQVIQSKTAIGSGMLYGRGLYKGVQNQLGYLPTKETDFI
                     FAVIGEELGLVGGLVLLFMYAVLFYRLIRIAKNAANMFGSLIVTGITAMQLFHIFENI
                     GMTMGLTPVTGIPLPFISYGGTFMLVNMISIGLCLSVGMKRGKIDLNEF"
     gene            complement(17075..18727)
                     /locus_tag="PLB33_02635"
     CDS             complement(17075..18727)
                     /locus_tag="PLB33_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOG62449.1"
                     /translation="MKIKLANTDEVIEVYENETLYRILKRVYKNDYFKYLGAKVNNKL
                     KGLCSKDFKENDRIEFIDITNPDGHRIYVRTLSLIYVKACKEIYNDIDVSINHSLNKG
                     LYTELQYKHLVTKKHLDAIKNKMQEIIDAKKNITTYFLSVDKAREIFSAQGMMDKVEM
                     LKFWNKDTIRVYELDGYYDTFYGYVAPNTGFVNKFDLRLFYPGIILNYPTREYNFELP
                     EYIEQKKLSKVFKEAEDWGEIMDVAYVGALNKKIVNNEIYDMIRINEALHEKKIAYIA
                     DEITSDKNIKIVLIAGPSSSGKTTLAQRLSIQLRVNGKKTYAVSLDDYFVDRHLTPKN
                     EKGEFDFETIKALDLDLFNEQLIDLMAGEEVQIPVYNFKEGSREYSREPVRLTNDHII
                     IIEGIHGLNDELTANIPQVNKFKIYISALTQLNIDRHNRISTSDLRLLRRIVRDYTHR
                     GNNAIKTMELWDNVIKGTEKYIFPYQENADAIFNSALVYELCVLKKYAEPIIMEIDED
                     SCFYSERQRLLKFLSYFLPIEDESAIPNTSILREFIGESCFR"
     gene            complement(18738..19646)
                     /locus_tag="PLB33_02640"
     CDS             complement(18738..19646)
                     /locus_tag="PLB33_02640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOG62450.1"
                     /translation="MKEIKITKNEEGQRLDRFLKKYLNKANQSFIHKMIRKKNIKING
                     LKAGPDIVLKTDDTVQLYLSDETIDKFRDKKTLQKTDIHFDIVYEDDNILVVNKPIGL
                     STQPDKTGKKNLVDEIKVYLDAKEENISFTFKPAVCNRLDKNTSGLVIAAKNYDALKQ
                     TNKAIRERKIRKYYMAKVHGIIKDDLELKDYLIKKDNKNMVKIINEYRENSKSVITYA
                     HPLKREGKYTWLEVEIETGRAHQIRAHLASIGHPIAGDKKYGKKDNEKYQVLHAYKLL
                     LDGFEGNLSYLNGMEIVSDISERKIL"
     gene            19848..20309
                     /locus_tag="PLB33_02645"
     CDS             19848..20309
                     /locus_tag="PLB33_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62451.1"
                     /translation="MKRIINAIRDFIYNITDYGLIITVIVVMTAILVWRFDLLFSRGI
                     EKEIIKDLPIINEPEVNEPIAEEPDPEDEEPEPEDETPTGLIAAISIPQGSFPSQIAE
                     ILLNNNLIKSKDEFLTRCVELDLDTRLQSGTFNIEVGTPLDDVIKIIANAY"
     assembly_gap    20315..20414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20443..20997)
                     /locus_tag="PLB33_02650"
     CDS             complement(20443..20997)
                     /locus_tag="PLB33_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62452.1"
                     /translation="MNYFKSKNSIFDDFFEHRDMLIIQHMNGDINKKEYLELNYKYML
                     EKNIKPFQRIDSFEKGMYNYQYYNMMAKYHRMIAQEIKDKGKHISYYNKYIKEADFYY
                     NEKDKTIFRLLRFLQFENIESYFIKMESSLLEGKLYEIVLKDFEYAVLHSKSLWLLDV
                     LKKEKVFTEKRKKSVIDYYVNQRY"
     gene            complement(21181..21594)
                     /locus_tag="PLB33_02655"
     CDS             complement(21181..21594)
                     /locus_tag="PLB33_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62453.1"
                     /translation="MVQLVCGHSGTGKTRRMIEMANNAIDKIHGSMVFLEASNRHIFD
                     LDYRIRYINTKEFGLTNDEEFQGFVCGVIATDYDIEQVYIDGLYKIAKSGKERLVPLI
                     ERLDYLSKTFDVNFIISVNHDIEDIPEGLRDRVID"
     gene            complement(21685..22599)
                     /locus_tag="PLB33_02660"
     CDS             complement(21685..22599)
                     /locus_tag="PLB33_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589008.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOG62454.1"
                     /translation="MKLYNKYSDYLKLKYGEKVYKLPVNIPCTCPNRDGTLGYGGCTF
                     CADVGTGFEMLDNSLSVKEQLNKNMDYISKKYKSGKFIAYFQNYTNTYMDIERFKNYI
                     NDAIMDNIVEIAISTRPDCISDEILEFLNEIKKQNVNITIELGLQTVNYHTLKNINRG
                     HTLAEFIDAVLRIKRYNFEICTHIILNLPGDTILDTVETAKILSVLKINQVKIHSLYI
                     MENTEMGRAYKNNEITLISKDEYVERVIVFLEHLDVDITVQRLIGRAPEKDSLFVNWG
                     MSWWKINDDILYKMKSENRYQGSKNKFV"
     gene            22745..22963
                     /locus_tag="PLB33_02665"
     CDS             22745..22963
                     /locus_tag="PLB33_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HOG62455.1"
                     /translation="MRIKEGIVPTILGTVVTATGVALKNTNLPESYRNGLIGFGLANI
                     AIGSAELVSSNWQHSRIMNRMTKMAPFK"
     gene            complement(23149..23340)
                     /locus_tag="PLB33_02670"
     CDS             complement(23149..23340)
                     /locus_tag="PLB33_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOG62456.1"
                     /translation="MRIITAIASILIIIGALNWGLYGLSKIDIVENLFGGWKSKFGRI
                     IYLLIGAAGLYSLLYVIFA"
     gene            complement(23419..24252)
                     /locus_tag="PLB33_02675"
     CDS             complement(23419..24252)
                     /locus_tag="PLB33_02675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOG62457.1"
                     /translation="MKYKMVVLDLDGTLLNSEKTISDKNAHILIELHKRGIHIVIATG
                     RNYYMAKTLIERLKFTEPVILANNGAVVRHYVNDEFIESNYLDFSTFERIYELGLKHD
                     LNPIIHVDEYNQGYDIIYECENYEEVYLGYIKKDYKRTRCKKFKTEEINNILSVCYFN
                     EYQKLLKLKSEIEKIPGKFNIILNQNISNWSLLELLNINGCKWCALKKYIDRLGIDAN
                     QVIAIGDDNNDIELIKNSGLGIAMKNATDELKKAADSISRFSNDESGVYYELSEIFKI
                     K"
     gene            complement(24469..>26303)
                     /locus_tag="PLB33_02680"
     CDS             complement(24469..>26303)
                     /locus_tag="PLB33_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG62458.1"
                     /translation="LSLILVLAMVFSLAACGNNQPPAEPETPETPETPETPEEPGIAD
                     SLTLHVGAYPDTIDPALNSAVDGATYIIHAFAGLVGYQQKEDGSLELYPDLAKELPVG
                     KERDDGKVEYVYELKDGLKWSDGSDLTAHDFVYAWNRAANPETAADYGYMFEVVDGYA
                     EMTEMDDEGNLVNPDAKLNVTASEDGKTLTVVLPVDVPYFNELVAFPTYMPVKAEVAE
                     GNDAWATAADTYIGNGPYKVVEFSQSQLIMQKNEYYHDADRVITNELVFAFNDDDTST
                     LANFQNGSYLFIDQVPNDEIKSLQEQYPDEFFIEGQLGTYYVSFNVNDENFKAFGEAD
                     KAKIRKALGLMIDRTYIVEEIGQAGQVPAAGFVPMGLTEPDGKTEYIAKNGPKGDGAG
                     YYSVDPDDYEKNCEEAVALLREVAESTGLYTVDENGVVSKEFPTIAYLTNEGTGHEAI
                     AINLQATFAQYGINMTIEKQEWATFLNTRKNGDYSVARNGWLGDYNDPISFLDMWITS
                     SGNNDSQFGKGEHATYAGYTYKGEGGKTWAESYDKIITEVKASKDSEERFALMHEAEN
                     ILMETGAICPIYYYTDIFMKSTDLQGAFASPLGYKYFMYSYVEE"
     assembly_gap    26304..26403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26404..27239)
                     /locus_tag="PLB33_02685"
     CDS             complement(<26404..27239)
                     /locus_tag="PLB33_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG62459.1"
                     /translation="MENNNNLLEVRNLKQYFDIPVGLLKSKPLKAVDDVSFSIRKGET
                     LGLVGESGCGKTTVGRSILQLYKPTGGEIIFKGKKIESKESIKQLRQNAQMVFQDPYS
                     SLNPRMTVGDIVGEPLDVMNMYKSRKERDKRVNELMEIVGLSSEHATRYAHEFSGGQR
                     QRIGIARALAVEPEFIVCDEPVSALDVSIQAQIINKFEDLQEQLGLTYLFIAHDLLVV
                     KHISNRIAVMYLGRIMELAPSDELYNHPLHPYTISLMSAVPIPDPSYARSHQRILLEG
                     DVP"
     gene            complement(27229..28251)
                     /locus_tag="PLB33_02690"
     CDS             complement(27229..28251)
                     /locus_tag="PLB33_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62460.1"
                     /translation="MSLLQVKDLYTSFFTSSGEVKAVNGVSFNLEEGKVLGIVGESGS
                     GKSVTAYSILQILTNPGRIVSGSIKLKGQELVGCSKETMQKIRGNQISIIFQDPMTSL
                     NPVFTIGNQLMEAILLHTNRNKEEAKARAIEMLKLVGVNEPEKRIEQYPHEFSGGMRQ
                     RVMIAMALACEPDILIADEPTTALDVTIQAQILDLMKELQKKLGMAIILITHDLGVVA
                     EMCDDIIVMYAGQISERGTADDIFYNPKHEYTKGLLKSIPTVQDEKQRLKPIGGNPVD
                     LLNMPAGCPFAPRCDNAMKICLTVNPVEMTIEGKHTSRCWMNVREGIENETLTIKQVE
                     EMYHGK"
     gene            complement(28262..29341)
                     /locus_tag="PLB33_02695"
     CDS             complement(28262..29341)
                     /locus_tag="PLB33_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62461.1"
                     /translation="MNNIDEMPKKNPLSFQLNLKDFEKATEDEKAQQVTMSESITFFK
                     DGMRRLRKNKIAMTCLTVLILIALIVTFVPMVYPYTYEQQLGVAQGKRIDKTYNNLKP
                     FEYGETELERIANGEKIFPHIFGTDAAGRDYAIRVIYGGRISLLVGFFAAIIVLIIGV
                     VYGSIAGYFGGKVDLFMMRIVDIIYSLPDMLIIILLSVVLNQVLQPKIEGTSLAKVGT
                     DMISIFIVFALLYWVSMARLVRGQILSIKQNEYILAAKSVGSSPWRIIKKHLLPNSMS
                     VIIISTALQIPSAIFTESYLSFLGLGVSIPMPSLGSLASDALTGIYSYPYRLIIPAIA
                     IWLIVLSFNLLGDGLRDAFDPKLKK"
     gene            complement(29360..30286)
                     /locus_tag="PLB33_02700"
     CDS             complement(29360..30286)
                     /locus_tag="PLB33_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62462.1"
                     /translation="MDSKLALYIVKRLTLAVITIFLIITITFFVMHEVPGGPFLSEKA
                     PSAAVTAALEAKYGLDKPKSEQYVTYLKGIMKGDFGPSIKQRGREVTAVIMQGFKVSF
                     KLGGTAAVIALCIGTVLGSVAAIFHNKTIDKIIMVVTTGLVAMPSFIIATLLLLVFCV
                     WMGIFPANGNEPGGLILPIISLSLYPMSYTTRLTRSSMLDVLSQDYIRTARAKGVKPA
                     KVIFKHALRNAVTPVVTYAGPMFAYIITGSLVVEKIFAVPGLGKEFVSSIINRDYTMI
                     MGTTIFLGTLIIIMMLISDILYKVFDPRIELG"
     gene            complement(30553..>30830)
                     /locus_tag="PLB33_02705"
     CDS             complement(30553..>30830)
                     /locus_tag="PLB33_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG62463.1"
                     /translation="GSYYPGWEYAKESNIRDICIEAYKDMFNKEPLVGAIHAGLECGL
                     LMDKIPGLDAISMGPDAYDAHSPNEHVSISSVENIYNLLCEILKRIK"
BASE COUNT         9013 a         6036 c         4605 g        10976 t
ORIGIN      
        1 catttgtctc aaccaattca acttttcttc atacttggtt ttatcatcta ttccttcttt
       61 gtatttccag tgggcagcaa taccatattc agcagttcta tgcatttcca aggtcctgat
      121 ttgaatttcg aaaggctcgc cttcaggacc tattactgtt gtatgcaaag attgatacat
      181 atttggttta ggcatggcta tataatcttt aaatcttccc ggcaccggtt tccaaagagt
      241 atgaacaata cccaatgctc cgtaacaatc ctttatattt tccacaataa ttcttatggc
      301 aattaaatca tagatttgtt caaatagtct gtttttataa tacatttttt tgtaaatact
      361 gtataaactt ttaggtctgc ccttaatgtc ggcttccatt cccgattcct gaagcttttt
      421 atttataata tcgataatgt gcgaaatata atcttttcgt ttttcgcttt tttcctgaac
      481 ttttttaagc aaatcttcat atccttcagg atctatatac tttaagctca aatcttccaa
      541 ttcccattta atggacgcaa taccaagacg gtttgctatg ggtgcatata tttcaatagt
      601 ttccattgcc ttttggtatt gtttttcctt gggcatatat tgtaatgtcc tgatattgtg
      661 aagtcggtcg gctaatttga ttataacaac ccttatgtcc tttgacattg ccactatcat
      721 ttttcttaaa gattctgctt ggcgctcttc tttgttcctg tacttaacct tgctcagttt
      781 tgtgacgcca tccaccatgt tggcaatttc ctcgccgaat tcccttttaa tttcatcgaa
      841 agttattccc gtatcttcca ctacatcgtg caaaagacct gcgcaaacag tctgtacatc
      901 catgtgtaag tcagccaaaa tgagcgcaac attataaggg tgaataaaat atctttcacc
      961 cgaaaatctt gtttgtgcat ttgaatgtgc catttcagca aaattatatg ccttagcaac
     1021 aatgcccaaa tcagcatttg gattgtatga ttcgatttga ctaataatat tgcctaacat
     1081 aactcaccta agttccttta catgcagcct agtgcacaaa ttttttagga cattcgtctt
     1141 atccgcaggc agtctcgcag ttttatttat catatgtgat taatgattcc accctgtatt
     1201 cttttagttt ttctcttcct tttaaatcct tcagctctat cagaaaacca agtccggcaa
     1261 cttcgccgcc taacttttca accagttttg cagtggcgta catggttcct ccggtagcca
     1321 acaagtcatc aataattaat accttctgtc cttttttaat agcatcctta tgaatttcca
     1381 aagaatctgt gccgtattcc aattgatatt catatttaat agtttctgcc ggcagtttcc
     1441 cgggctttct tacaggtaca aatcctgttt ttaataaata tgcaagaggt gttgcaaata
     1501 taaagcctct tgattccggt cctgcaatta tgtcgacatg aacatcttta aacctttctg
     1561 ccatttcctt gacgcattca tgcagagctt cagaatcttt cagcaaggtt gtaatgtctt
     1621 taaaactaat ccccttagcc gggaaatcct ctataacacg aattttttcc tttaaatcca
     1681 tttcttcctc caagcattta tttttttata tacaatatgt cattttttaa agcaaactga
     1741 atattatcag cctttttcaa catgcatatt atttcctctg ctgttatttt tgtattatat
     1801 cttttattaa gtttttctac aagtataaat aaatccgtct taattatttt ggattttgtt
     1861 ttgttaatca gaaactccaa tgctttcagt ttattaacat caattttgta atcaaattct
     1921 tcatgctcct ctacgtcatg taaaaaaagc tgtaagcttc tgctgccgtt atatatattt
     1981 tcacttaaag atacaatgta ggattgaggc atggtaagta ttttttcaag tattccgatt
     2041 ttgcagaacc ctattaccgg aatgtgcctg tctccgtcag acagcataaa ggatatatgt
     2101 ttcccttctt tgcccacttt ccttatattt tttatcgaca catctcttag ggctagcaag
     2161 ggtttcggat tgcctgaacc gaaaggctca aatctgttta tttcatcata aagccttaaa
     2221 tttatgtctg atatattcag aattgcatct gctctaattt gattatattc atcgtcagta
     2281 gtggagccga tataattgtt cagttcattt gtgaaggaat taatattttt ttccaaaatg
     2341 gtaagacctg ccgctaattt atggccgcca tacttttcga gataaatatc tgtatgctta
     2401 aatgattcaa atatatcaac atgctctaca cttcgagctg aaccttttcc tatcccatcc
     2461 tttacggata taacaacaca gggcttataa tatttttcag taagtcttga ggcaacaatg
     2521 ccaagcacac cttcatgcca attatccccg tatactacca ttacattatt tttatacagg
     2581 aaatcagatt caattttctt tattgcttct ttatatatta tattttctgc ttcttttctt
     2641 gcattgttta aactgtccag ctcagatgca agcttttctg cttcttcttc atcttttgta
     2701 agcatgagct taattgcttt ttttgcagta tccattcttc ctgaggcatt aatccttggg
     2761 gcaagtataa aaccaatatg ataggattta atttctccgt ttatgcctga tacttcaatt
     2821 aattttttaa gccctataat cttagtttta tttatttttt ttaaaccttg actggcaata
     2881 atcctgttgt cctttaccag atcaactatg tcggcaatag tgccaagaca tgcatattcc
     2941 aggagcacat cctcaatatt atctattttt aaaaacttat ttaaatttat caaaaattta
     3001 aaagatagcc cggctgcaca taaatgcttg ttttcggacg gacaatcttt ttgcttcgga
     3061 ttaattatgg cataagcatt gggaagctct tccctgctct catgatggtc aattataatt
     3121 acatctttat caagctttgt aatttcgtca attttttcaa tttcagctat gccgcaatcc
     3181 acggttatca gaagctcaaa atttatttca ccgcttttaa cactgttaac aaaatcggag
     3241 cttattccat atccttcgct ttctctttcc ggaacataat aattgacata agcacctgct
     3301 ttttttaaaa aaacaacaaa ctggctgatg gaagtaactc catcaacatc gtagtcgccg
     3361 taaatcagaa tcctgtcatt attattaatt gcttcaataa ctcttttgca gcccttatgt
     3421 aaatctttgt aattttcagc tctttcaaaa tatttgaagt tcggttcaag aaattgctct
     3481 atatcatcaa aagattttat atttctgttt aaaagtattt ttgcagatgt ttttgaaaga
     3541 ttaaaccttt tgctgattct ttctatttca tcattattat attcattgac tttcaacttc
     3601 actggtttca ttgtctatct gtgcagcacc ttcatctgta attaccgtat ccccttcaat
     3661 tttttcaata ttttgagcct ttaaaatttg tatttcttgg tttttagcaa ctaattcttc
     3721 atcctgcaat ttaattttgt cttcaagcag tgtaactttt ttactgaaat cggctatttc
     3781 attctgaagt ctttgttttt cggtttcaag agcctctata tttgtttgga attgagcttt
     3841 ttctgattct aatgctttca tatctatttc atgcttagct ttttcgcctt caagagtttt
     3901 gagtttgttc tttaattcat tatactcgga tcttttctta aattgtctgg tagtacctaa
     3961 tattaagact accacggcac caagaatcag acaaatcatc attattacag ctaagggcat
     4021 ttcatatctt gcaaaaaata aatcaacagg aaccggtgtt ccattttgta ttgcaaaaat
     4081 cgcaacaaaa atcgcaatta taagaattac tataaaacca atttgcatat taatactcct
     4141 ctcttgtctt tatttttatt atatctgtat tatactatat taccatatac aattaccact
     4201 gtttttttta caggtatggg gacaaccttt aattgaagga aagtcttaat ttattttagc
     4261 ttttatataa attgcgcact ctattcgttt tttttctaac tcgcatccaa catcatatgg
     4321 gacagtaaaa tccttgttaa aattatatgc attggcatga caaccaccgc tgcaaaagta
     4381 ttttgcccaa caatctctgc acgcaggttt atcatttacc gatacatttt taaatttatc
     4441 ggcaagagcc cgatttgtaa taccttcctc aacattaccg ataataaatt cttcattacc
     4501 tacaaattga tgacaaggat ataaatcacc tgacggagtt acggcaacat attcagttcc
     4561 tgctccgcag ccaatggatc ttttgtaaat gcagggacct ccctccagtt cgatattaaa
     4621 atggaaaaac tgaaaccttc tatctttact tttatatgat tcaatataat attcagctaa
     4681 tttttcatat tcttttttta aaatatccac atgctctttt tttagtgcat atttttcctc
     4741 atccttagca acaacgggct ctacagaaat tttatcaaaa cctaattctc tcatatgctt
     4801 gacatcttcc gagaagtcta aattattgga agtgtatgtt cccctggcaa aataatcctt
     4861 actgcctctt ttgctgataa atttcttata attatcaact attaagtcat agcttccttt
     4921 tttatttatt gttttacgca ttctgtcatt tgtttctttt cttccgtcga tgcttaaaac
     4981 cacattgtcc atattttcat taatatagtc cattttttct tcatctaaaa gaacaccgtt
     5041 ggttgtaacg gtaaatctga aatgtttgtt atattctttc tccaagcttc ttccgtaatc
     5101 aaccagtttt ttgattacgt caaaattcat gagaggttct ccgccgaaaa aatctacttc
     5161 taagtttttt ctgttaccgg agtttttcac caagtaatca aatgcctttt tccctgtttc
     5221 aaaacttaga aatgcctttt ctccgttata tgtcccttgt gaagcaaagc agtattcgca
     5281 tcttaaattg cagtcgtggg tcatattcaa acacattgct ttaataaccg gctttattgt
     5341 atttacatat ttaatatctt cggtatagag cattttattg tctattaaat attgtatttc
     5401 cgataaagct tctctaactg tgctttctcc gtattcattg tataactcac tgtatttatt
     5461 ttcatccttg aatttttcat tcattaataa atcaaatact atcttatcga cggcatgtac
     5521 cagtccggtg cttgtatctg ttgcgaaata taaatctttt atattaaata catgtatcat
     5581 acttcctccg aaattttgct aattaaaaat aagtcatcct gaacgaagct atgtagtgtg
     5641 ctgcctctaa ttgttagagc acctctcaag ctgcgccttc agaatgacaa actctgtcta
     5701 aacaataaac tatactacaa ttattctttt tcacattttt gatttccgac agtacaagat
     5761 gttttgcaag ctgactgaca tgatgtctgg cattctccgc atccctttgc gcttaaatta
     5821 tccgttaact tataattatt aagtgttttt atgtatttca tgacaacctc cttataaact
     5881 taatagatta tagcattagt tgaggttatt ttaaagtatt ttttttattt ttttattact
     5941 atttgttttt tatactcttg tttttcatgt taacaccgat gattcctccc agtattgaca
     6001 ttattagaat caccccgttg tatagtattc ttatttcgga cgggtatctg aataagactt
     6061 ttatcaataa gaccattgca aaatatacta tccaaatttc aattcccctt aacagccctt
     6121 tttcatgaac acttcttgaa tacaacattg aaacagcaaa tatgcatatt ggaacaatga
     6181 agctgtagac tatttcaaaa ccgggattat ttgaaaaata atatatataa acggtcagta
     6241 taaaataaat tattaaagat aaaataaata aaaacagaga atacttaaac aatttaccta
     6301 acttcataat acctccttga gcatgactta ctaaataata ttcatccgat aataaaaaag
     6361 aacattaata tattacttga caaagatttc tatatttgat aaagttatct tgctaaaagg
     6421 gagtagtttg aaattttcag tcaacaacac ggaatatatg tattctggct gaaaatcctc
     6481 gggcaactta ggaaacgaga cttttggtat atctggagta ggtatgctaa aagtcttttt
     6541 tattaaacag ttgtgaataa tcttaaagga gtagacaatg tattaccgaa aaagcataac
     6601 agaaacatgc gaagaattaa acaccgctca aagtaccggt ttaacccaag aagaagcaaa
     6661 aaagaggctg ttgtcagacg gtcccaatgc attggttgag aaaaaggcaa aaacaaagct
     6721 gcagatgttc ttggcccaac taaaagatac actgatttac atattgtttg cagctgcagc
     6781 aatatccata gctctaaacg aaataacaga tgcaataata attctattag ttgttttatt
     6841 gaatgcagta ataggtatgg ttcaagaatc aaaagcagaa gctgcacttg aagccttgaa
     6901 gaatctttca agcccctatg caatggtaag gcgtgagggc agggtaatgg aaatacctgc
     6961 aagtgagctg gttgtgggag atttagtaat actcgaagcc ggacgaatta ttcctgccga
     7021 tttaagactt attcagtcgg taaacttaaa aatcgaagaa tctgctttaa ccggtgaatc
     7081 cgtacccgta gacaaaaatg ccgatatggt ggctgacaag gaagtgggaa tcggagaccg
     7141 cctaaatatg gcattctctt ccacaagtgt ttcgtacggc cgtggtgaag gtgttgtagt
     7201 ttataccgga atggataccg aaataggaaa aatagctgca atgataagtg aaagcaccga
     7261 agaattaact ccccttcaaa aaagactcaa tgacttggga aaagtattag gtatagttgc
     7321 cgtagttata gttgtagcca tgtttggaat agctgttctg cagggaagag acataatcga
     7381 aatgtttata acagccattg ccttagccgt agctgccgtg cctgaagggc ttactgcagt
     7441 tgtaacaatc gtattggctc ttggtgttac aaaaatggta agggtaaata ccatagtccg
     7501 caagcttccc gcagttgaaa cgttaggagc agtcagcatt gtatgctcag acaaaaccgg
     7561 tactctcact caaaataaaa tgaccgtaac aaaagtatat ttagacggtg aaattaaaaa
     7621 taccgatgac ttatcttatg aaaacaacaa aatatttttg gacggcttta ttctttgcaa
     7681 tgatgcctcc atagctaata acactcgtat aggagaccct acagagttag ctctcttgga
     7741 tatgggagac aaaattaatt tgtcaaggga aggattagaa aaaactaatc cgagaatcaa
     7801 tgagcaatcc tttgattctg caagaaaatt gatgacaaca gttcataggg accaagacgg
     7861 caaggtaatg agctacacta agggtgccat ggacatactt ttgaacaggt gcaataaaat
     7921 atatataaac ggcgaaacaa aagatataac aaatgaacac attgaagaaa taaacagggc
     7981 tgccacagaa atggcaaaag gagctcttag agtattggct cttggaatca aagaagatga
     8041 cgattcagca agcgaagaag atctcacatt tgtgggcttg gtaggaatga tagaccctcc
     8101 tcgacccgaa gcaaaggatt cggtaaaaac tttaagaagt gcaggtatca ggacaataat
     8161 gattacagga gaccacaagg atactgctct tgctattgcc aaggagctcg gtattgcaga
     8221 agatgaatca caatgcataa ccggcagtga attgaatgag atgactcagg agcaattaaa
     8281 tgaaaaagtt tgtgatataa gagtttttgc aagagtttca ccggaacaca aggtaatgat
     8341 agtaaaagca tttaaatcaa acggaagcat agtttctatg accggtgacg gtgttaacga
     8401 cgctccatca ttaaaatcag ctgatattgg tgttgccatg ggcataacag gtactgacgt
     8461 agcaaaaggt gcttctgata tggttcttac ggatgataat tttgcaacca ttgaaaaggc
     8521 agttgcggaa ggaagaggta tttacaataa tattaaaaag acggttctat tcttgttatc
     8581 ctccaatttt ggtgaagtta tatcaatgtt ttcatctatt gcggcaggac tgatagcacc
     8641 gctgcagtcc atacacattc tttgggttaa cctgataacc gactcgcttc ctgcattagc
     8701 attaggagtt gacccgaaga gcaaagaaat aatgaatgaa aaaccaagag acccaaatga
     8761 aagcttgttt gctaagggag gtttgatatt taccgtttac aacggaataa tgattgcatc
     8821 attgactctt gcagcctttt tatggtcacc cgtcattcat ttaaatgaag caggaatacc
     8881 tgtaaccttg ggaaatatac gtgatttatt taacggtgtt ctcagtatac ccggagtaga
     8941 tttgcctgat attattaggc atgctcaaac atatgcattt acgacccttg gtgtttctca
     9001 gcttttccat gccgtaggta tgagaaatta taacaaatca ttgttccaaa tgagccacac
     9061 tgataatctt gcaatgattg gagcattttc tttaggtttg ctgctgcaga tacttgtaac
     9121 ggaagttcat ttccttaccg aagtgtttga aacaagccag ctttcattga aagaatgggc
     9181 aaatctgata ttagtatcag ctgttccttt actgtcacac gaagtaattg ttatggggaa
     9241 gaaaattttt aagaaataac ccattattgc tataagtcag tgtcatagac attaataaca
     9301 tttttgtcat gagatccttc gctggcgctc aggatgacaa ttaaaaaaag agcatcagct
     9361 cttttttatt tgtcatcttc tttatcatca acagtttctt caatttcttc tttaacaact
     9421 tcttctttag ctgctttttc tttgcccttc tttgtaacac ttcctattgc ccatttagaa
     9481 aactcaatcc tttgtttacc aaagggcatt tctaatgtaa caatttcatc attgatttta
     9541 acaacttttc cgtgaatacc gcctatggtt attacctcat cccccgttgc caagctgtct
     9601 ctcattgctt tagtttcttt ttctctcttt ttctgtggtc ttataatggt gaaatacatt
     9661 atcgcaaaga aaacgaccaa cattaaaata ccgctatatt cctgtggcat aattcctcct
     9721 atttactttt ttaatatcca taattctcat aaaattctct tttgaacttc ataaaattat
     9781 cttccgctat acttattcta atacctttca ttaaattaat caagaaaaat aaattatgaa
     9841 ttgatgcaag cctagcgccc aatatttctt tttcgccaaa taaatgcctt atatatgctc
     9901 ttgaataatt tctgcaagta tagcagctgc attcctcatc aatgggactg aaatcatttt
     9961 tatattttgc attcttaatt gtaagctgtc ccttgcttgt taaaaacgca ccatgccttg
    10021 ccatccttgt aggcaagacg cagtctgcca tatctattcc tctttcaacg gcttcaaaca
    10081 aataatcagg gctgcccaca cccattaaat atcttggttt atccttcggc aaaagctcta
    10141 cggtataatc cataacttcg cacattaaat ccctcggctc tcctacactt aagcctccta
    10201 atgcataccc aggaaactca atatccgtaa tctgtttggc actttcaatc cttaaatcct
    10261 tgtacattcc tccctgaaca attccaaaca aggcttggct ttttgtattt ttgtgatagt
    10321 ccttgcatct ttttgcccag cggcttgttc tttccatgga ctttttaaca tattcataat
    10381 ctgcaggata tggtgcacac tcatcaaatg ccatcattat gtcagagcct aaataatttt
    10441 gtatttccat tgaaacttca ggacttatga aatgctttga gccgtcgatg tgcgagcgga
    10501 attcaacgcc ttcttccctt atttttctta aattctccag actgaaaacc tggaatccgc
    10561 cgctgtcggt taaaatgggg ccgtcccagt tcataaattt gtgaagccct ccggcttctc
    10621 ttattaattc atgaccgggt cttaaataca agtggtaggt atttcctaat ataatctcag
    10681 cccttagctg tttcaattct tcaggtgtca ttgccttaac cgttcccttt gttccgacag
    10741 gcatgaatac gggtgtttct ataatcccat gtggagtata tattctcccc agtcttccct
    10801 tgcattcctt agattctttt attaattcaa atttaaacat aatccaccta ttttataaac
    10861 attgcatcac caaagctgaa aaatctgtat tctttttcta ctgcttcacg gtatgccttc
    10921 ataatatttt cttttcctgc caatgcgcta accagcataa tcaaagttga ttccggaaga
    10981 tgaaagtttg ttactaaact gtcaaccacc ttgaatttgt atccgggata aataaaaata
    11041 ttcgtccatc ccttgctttt gtttactatt ccttcgtcat cagcaactgt ttccaatgtt
    11101 ctgacacttg tggtacctac ggttataacc ttgtgtcctg agctcttggc aagattgatt
    11161 ttatttgctt cactttcgtt taattcataa tactctgaat gcattatgtg ctcattgatg
    11221 tattcggtct ttacgggtct gaatgttcca agccccacat gcaaggtaag ccttgctata
    11281 tttacatcct tattttcaat ttcttccaaa agcttatcat taaaatgaag tcctgccgtt
    11341 ggagcagctg cagagcccaa gtgttttgca tagatagtct gatatctgtc cttatcctta
    11401 agtttctcgt gtatataggg cggcagcggc atttcgccta atctatccaa tatttcttca
    11461 aatatgccct catattcaaa ttcaattatt ctgttgcctt cttcaaggat atcgataatt
    11521 ttccccgtaa gcaatttttc atcaaaaaca atttctgtcc ctgttctcgc ctttttaccg
    11581 ggcttaacta atatttccca ctttttatca tctattcttt tcagtaaaag aatttcaatt
    11641 aaagtctgcg tatctttttt atttccgaat aatcttgcag gcagaacctt ggtatcgtta
    11701 attactaaac agtctcctgc atttaaatat tcaactaaat taaaaaaatg cttatgctcg
    11761 attttcccgc tttttttgtc aagtaccaaa agcctggaat ggtctctttc cttaataggt
    11821 gtctgtgcta tgagcctttc gggcaattcg taataaaagt cgcttgtttt aaaattcgtc
    11881 atttaactgt tactccggta taataatgtt ttattatttc ttcataggta tatccttctt
    11941 cagccatttg cttagctccg tactggctca ttccaatacc gtgtccccag cctcttcctt
    12001 caaaattata ttgctctgaa ttagcgtcta ctgttgttac accatttgaa cttataaagg
    12061 ataatttttc cctgtttatt ttttttattc cgtcgtcggt tattgcatgc ttacctgcaa
    12121 tacttccggt ttcaaactct atttcatttc ttgtttcttc tgtttcctct tctgttatta
    12181 aatcaagtac tcctcttgat ggcggtgttt catcttttcg attttcatct aaagaaaagt
    12241 attctgttat aaagcaaaat atatttccgt ttactataga aaaccacgag cttttcaatc
    12301 ccaaaagaag ccttgcattt tccttcttat atgaaatttc tcctgcatcg gttaaaaata
    12361 tacattcaac taccctgtta ttttcagaaa ctttctttat ttgaattccg tacaattcat
    12421 ctacgtcatt gccatcaaga tttaaatttc ttattatgtc ttccttgtaa tacgtggtct
    12481 gccaggtggt atgaggtgag cctgaattat tggaaaattc gtcatttaca cctcttaaat
    12541 acggcagctt ttcataccat acattttcgg agttttctgt agacccgccg ctggttgaat
    12601 gatagaaggc ctgaattaat tcgccgtcgt aataaatcat ttctcccctt gtttcatcca
    12661 cagctctgtt tgtagatgtc ttttcatagt cataacccat atatacctga ctgttttgat
    12721 tgtcttccat atcgtagatt tgtcttggct ttatgttgta caccgcatag gttcttgcgg
    12781 ccagagcttg agccttcaat gcttctattc cccaagatga aggtatttca ttcggaacaa
    12841 ctccatagag gtagttttca agctctacat gatttatggt taaaagtttg aagtcttcaa
    12901 ttatattcag tcttattctt cctctgtagg ctctgttgtc aaccttaatc atttcatatt
    12961 cttcatcttg gttgtacgaa gaaaaataaa caggcatgtc ctttttatac atgaatacag
    13021 gtatattctt tttgttgtat gcaattatgt tttccaggtt gccattcatc gattcagcaa
    13081 tatagccctc atcatttaat tcttccgcta aatcctgtga agagctttta tccgcaaaat
    13141 ttcctccggc aataatatat ccgtcttcaa tcagataagg ataaaactct accttaaact
    13201 cttcatttaa ttcttctgct atcaaataag cttcttcata ggaagaatat atttcatcct
    13261 gcagcactgc atgatatgga ccataagttc cttggctctt gtccgataaa taataattgc
    13321 ttgaataata tgtatcaatt cttaatgtaa ggctgttatt aaggcaaaac agctcatctt
    13381 catcatcata caatacgatt tcatcaaatc cttcaaaaga tacctgctcg ttaaaaagtc
    13441 tcgggtatct tattcttacc cttacatata tgtcatcttt atttttacca aaagcggttg
    13501 ttggaaataa aaaagtaagt aaagcagtta ttataaatag tctaatcaaa ttcttcttca
    13561 taatcgtcct ctaatatatt aatttgatct tgtattttcc ttattttttt aacgtgcctg
    13621 taacccgact cggatacaac tcttcctctg ggtgtacggt ttaaatatcc tatctgcaaa
    13681 agataaggct catatacatc ttctattgtt cctttttcct ctccgatgga agctgccaat
    13741 gtatcaattc cgacaggacc tcccttgtaa aagtcaatta tagtgtttaa aattcgtctg
    13801 tctaatttat caagacctat ttcatcaatt tcaagcattt taagagcttc atcagcggtt
    13861 tttaagtcta ttatcccttc gcccttcaca attgcataat ctctgaccct ttttaacagc
    13921 ctgttggcta ttcgaggagt tcctcttgag cgttttgcaa tttcttctgc tcccttttct
    13981 tcaacttcaa tattcataat acttgctgag cgcaatacta tttcttttaa atcttccggc
    14041 tcatagaaat ccaaattgca cataacccca aacctgtccc ttagtgggga tgcaagcata
    14101 ccggaacggg tagttgcccc gataagggtg aatttaggca aatcaagtct tattgatctt
    14161 gccgaaggac cttttcctat tataatatcc aaggcaaaat cttcaagtgc aggatacatg
    14221 atttcttcca cagtcttatt aagcctgtga atttcatcaa taaacaatac atcattttca
    14281 ttcagatttg taagtattgc agctaaatca ccggctcttt ctattgcagg tcctgaagtt
    14341 acccttatat taactcctaa ttcatttgat atgatattgg ctaatgtggt cttccctaac
    14401 ccgggaggtc ctgaaagcaa tacatggtca agaggctgat ttctaatttt ggcagattca
    14461 ataaatatgt tcaaaacttc ttttactttg ttctgcccta taaattgagg gagtttctga
    14521 ggtcttaagc taagctccaa ttcctcttca caagaattaa atgttcttgc aactatttca
    14581 ttttctcttt caaacatatt atccctgcct gttcatattt tttagtgctt ctttaattat
    14641 atcattttca gacttgcctg acagctccat attccccaat gttcttttga cctcggcata
    14701 gttaaatccc aaagatgtta aggcttcgat cacatcggac atttcgctgc ttacaacaat
    14761 ctcgcttcct ttgagttctt caactgttcc aaccttatcc ttaagctcca aaattatttt
    14821 gctcgctgtt tttttgccta tccccggaac cttggacagt ccggatacat cttcttttag
    14881 aataatttct tttactgtat ttacctcata tgttgacagt acggaaagtc ctgctctagg
    14941 tcctattccg ttaacactga ttagtttttt aaacaaatct atctcgtcag aagtaagaaa
    15001 tccatataaa gcaataatat cttctcttat gtgcatgtat gtaagtataa taattttctc
    15061 tttctcactt attttttcta aggtcgaaaa agaagtattt atataataac ctatattatt
    15121 gttatctaat atcaagtagt cgatgccttt ttttactact tcacctttta tgtagctaat
    15181 catatcttcc ctcaatattt attaatttta aattcttttg caaatttaag cgaatgtgca
    15241 tgacaaattg ctgccgctaa tccgtcggcc gcatcatcag gctttggaat ttcatttaat
    15301 ttaagaatta ctcttaccat ttcctgtacc tgtttttttt cagctctccc ataaccgact
    15361 attccctgct ttatttgaag tggtgtatat tcataaagag ggacattttt gttttctgca
    15421 gccaaaagat gcactcccct tgcctgggca atagccattg cggtttttgc atttttatta
    15481 tagaacaatt cctcaattgc aacggcttca ggcttgtaca tatctataat agatatataa
    15541 ctttcataaa tatgtttgag cctcttcggg aattcttcac agctttcggt aaatatgcaa
    15601 ccatattcta ttactttaaa tttatttcct gaataattta atattccgta gcctgatatt
    15661 gccagccccg gatctatacc aagtatcagc atatttcgtc ccctcaatta ttatataaaa
    15721 ttatagcata catttgttct aatataaaga acttaattca tgaaatattt gttttccatt
    15781 gttttaagat gtgatataat tggggtaatc taataaaatt acgtcatcct gagtataagc
    15841 aaggaagaca tggagtaaat atgtttaaaa aagaaagtaa acttaaaagg tttgatttta
    15901 tattgttaat tacaacaata cttttatgta tatatggatt tatagcaata aacagtgcca
    15961 caatgagcaa agcaacaggt agcgagccat ttttaaaaac gcagataata gctttcggac
    16021 ttggtatggt tgctttattt gtgttgattt ttattgatta tgatatttac ggaagccttt
    16081 atattccgat atatgtgatg accaatatac ttcttttgta cacattgata aatcccgttg
    16141 aagcttccga atggggagat gtaaggtcat ggatagcaat aggtcccata gtgtttcagc
    16201 cttctgaaat agctaagttt ggaataataa tttctctgtc aaagtttatt gatatccaca
    16261 aagataatat taaccagccc gtagtgttat taaaaattat tttgttcgct tttttaccga
    16321 tagcattaat tttaatgcaa ccggatttag gaactgccgt tgtattcata ttttttgtgg
    16381 caataatgat ttatatttca ggtattgaca gaaaatatgt gctttctgtt atgataatcg
    16441 gtttaatatt aattattgcg ggactcagta tattctttca gataatggaa gattacacct
    16501 taaatgaaga ttaccgcctt gacagaattg taaccttctt ctatccggaa ttagacccgg
    16561 atgatacggg ttatcaggta atacagtcta aaacggcaat cggttcggga atgctgtacg
    16621 gaagaggatt gtacaaaggt gttcaaaacc aattaggata tcttccaacc aaggaaacag
    16681 attttatttt tgcggtaata ggcgaagaat taggattagt tggaggctta gttcttttgt
    16741 ttatgtatgc cgtcttattt tacagactta tcagaattgc caaaaatgct gctaatatgt
    16801 ttggctcatt aatagtaaca ggcattaccg caatgcagtt gttccatata tttgaaaata
    16861 taggaatgac aatggggcta acgcctgtaa cgggaattcc tcttccattt ataagctatg
    16921 gaggtacctt catgcttgta aacatgatat ccataggtct atgcctgtct gtaggaatga
    16981 aaagaggaaa aatcgattta aatgaatttt aaacgtgtat ataaggtgcc aagcaccacc
    17041 aatggtacca ccaatggtgc ctagcacctt atcattatct aaagcagctt tctccaataa
    17101 actcccttag tattgaagta ttaggaatag cgctttcatc ttcaatagga aggaagtagc
    17161 ttaaaaactt caacaatctt tgtctttctg aataaaaaca gctgtcttca tcaatttcca
    17221 taataatggg ctctgcatat tttttaagaa cacacaactc ataaactaat gccgagttaa
    17281 atattgcatc cgcattttct tggtaaggga aaatgtattt ttctgtacct ttaatcacat
    17341 tatcccacag ttccattgtt ttaattgcgt tgtttcccct gtgggtgtaa tctcgtacaa
    17401 tacgtcttaa tagtctcaaa tcagatgtcg aaatacgatt atgtctatcg atgtttagct
    17461 gtgtcagtgc acttatataa attttaaact tattaacttg cggtatatta gctgtaagtt
    17521 catcattaag cccgtggata ccttcaatta ttataatatg gtcatttgtt aaccttacag
    17581 gctcccttga atattccctg ctgccttcct taaaattata tacaggtatt tgaacttctt
    17641 ctcctgccat caagtctata agctgctcat taaaaagatc caaatctaac gctttgatag
    17701 tttcaaaatc aaattctcct ttttcatttt ttggtgtcag atgcctgtca acaaaataat
    17761 catccaagga cactgcatat gttttcttgc cgttcactcg taattgaatc gacaatcttt
    17821 gtgctaatgt tgttttacct gaagaggaag gacctgcaat gaggactatt tttatatttt
    17881 tgtcacttgt aatttcatcg gcaatatatg caatcttttt ttcgtgaaga gcttcgttaa
    17941 tccttatcat atcatatatt tcattattga cgattttttt gttcaaagcg ccaacatatg
    18001 caacatccat tatttccccc cagtcctcag cttccttaaa tacttttgaa agtttctttt
    18061 gctctatata ttcgggaagt tcaaaattgt attcacgggt gggataattt aatataattc
    18121 cgggataaaa caatcttaaa tcaaacttat ttacaaatcc tgtatttgga gcaacatatc
    18181 cgtaaaaggt atcataatat ccgtccaatt catacactct tattgtatcc ttattccaaa
    18241 acttaagcat ttcaacctta tccatcatac cctgagccga aaatatttcc cttgccttgt
    18301 caacacttaa aaagtaagtt gtaatatttt ttttggcatc aataatttcc tgcattttat
    18361 tttttatggc atcgaggtgt ttttttgtta ctaaatgttt atattgaagc tccgtataaa
    18421 gtcctttatt taaagaatga ttaatggaaa catctatatc gttatatatt tccttgcatg
    18481 ctttaacata aattaatgac aaggtgcgaa cataaattct gtgaccatcg ggatttgtaa
    18541 tatcaataaa ttctatcctg tcgttttcct taaaatcttt gctgcaaagt cccttaagct
    18601 tattgtttac cttagcaccc aaatatttaa aataatcatt tttataaact ctcttaagta
    18661 tccggtataa tgtttcattt tcgtatactt caattacttc gtcagtattt gctaatttaa
    18721 ttttcattcc taacctccta caatattttt ctttcactga tatccgatac aatttccatt
    18781 ccgtttaaat atgataaatt gccttcaaat ccatcaagca acaacttata cgcatgcagt
    18841 acctgatatt tttcattgtc ttttttcccg tattttttgt ctcctgctat gggatgtccg
    18901 attgaagcta aatgagcccg aatttgatgt gctctgcctg tttcaatttc tacttccagc
    18961 catgtatatt ttccttctct tttcaaggga tgtgcatagg ttatgactga ttttgaattt
    19021 tctctgtact catttataat tttaaccata tttttattgt cttttttaat aagataatcc
    19081 ttaagctcca aatcatcctt aatgattccg tgtacctttg ccatgtagta ttttctgatt
    19141 tttctttctc ttattgcctt gtttgtctgt ttcagggcat catagttttt tgccgctatt
    19201 acaaggcctg atgtattttt atccaagcgg ttgcacacgg caggcttaaa tgtaaaactg
    19261 atattttctt cttttgcatc taaatatact tttatctcat caactaaatt tttctttcct
    19321 gttttatcag gctgagtgct taggcctatg ggtttgttta ctactaatat attatcatcc
    19381 tcatatacaa tatcaaaatg tatatcggtc ttttgaagtg ttttcttatc tctaaattta
    19441 tctattgttt catcagacaa atacagctgg actgtgtcat ctgtttttaa aacaatatcc
    19501 ggtcctgctt ttaagccgtt tatctttata ttctttttcc taatcatttt atgtataaat
    19561 gactgatttg ccttatttaa atatttcttt agaaacctat ccagtctttg accttcttca
    19621 tttttggtaa ttttaatttc tttcatgtgt tatcctttac tttttcttag gtaaattaaa
    19681 tatttgtccg gagggtacgg gcactatgtt tctctatact attataacaa atattttatg
    19741 aaagtatatg cattttacag tttttacaat tatttattaa atgtggatat tgtatgcatc
    19801 tcatttttgt gttaatataa taagagcgat aagctaagag gtgacaaatg aaaagaataa
    19861 ttaacgcaat aagagacttt atatataata taactgatta cggacttatt ataaccgtaa
    19921 ttgttgttat gacagctata ctggtttgga gatttgattt gttgttcagc agaggaattg
    19981 aaaaagaaat aattaaagac ctgcctataa taaatgaacc tgaagtaaat gaaccgattg
    20041 cggaagaacc ggacccggaa gacgaagagc cggagccgga agatgaaaca ccgacagggt
    20101 taattgctgc cattagtatt cctcaaggct cattcccttc acaaatagcg gaaattctgc
    20161 taaacaacaa tttaataaaa agcaaagatg agtttcttac tcgctgtgtt gagcttgacc
    20221 ttgatacaag actgcagagc ggaacattta atattgaagt aggaacacct cttgatgatg
    20281 ttatcaaaat aattgcgaat gcttattaaa tatcnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnacaata aaagaaggtg tgtttccaca ccttaatacc gttgatttac
    20461 ataataatca ataactgatt tcttcctctt ttctgtaaat actttttctt tttttaaaac
    20521 atccaaaagc cacaagctct tagaatgaag tacagcatat tcaaaatcct tcaatactat
    20581 ttcgtaaagc ttgccttcta ataatgatga ttccatttta ataaaatatg attctatgtt
    20641 ttcaaattga agaaacctta acaggcggaa aattgtctta tccttttcat tgtaataaaa
    20701 atcagcttct tttatatatt tattatagta actgatgtgc tttcccttat ccttaatttc
    20761 ctgtgctatc attcggtggt attttgccat catattgtaa tattgatagt tgtacattcc
    20821 tttttcaaag ctgtcaatac gttgaaatgg tttaatgttt ttttcaagca tgtatttata
    20881 attaagctcc aagtattctt tcttatttat atctccattc atgtgctgaa ttatgagcat
    20941 atccctatgc tcaaaaaaat catcgaatat agaatttttc gatttaaaat aattcatgta
    21001 atcactcttt ctaatttagt acaatgccta ttctaacaga aaaatgttac attagcaagt
    21061 gctatatacc atatatatgg ttcttagaga agacgtttct tacaagatat gctttaattt
    21121 gctttaaaga agaggtatcc ccggatataa taaccgccgg tcaaaaaccg gcggcataaa
    21181 ttaatctata actctatctc tcaatccttc tggaatatct tcaatatcat ggtttacact
    21241 tataataaaa ttaacatcaa atgtttttga taagtaatct agtctttcta ttaatggtac
    21301 caatctttcc ttgcctgact tagctatttt gtataatccg tctatgtaaa cttgctctat
    21361 gtcgtaatca gtagcgatta ctccacatac aaaaccttga aactcttcat cattggttaa
    21421 tccgaattct tttgtgttaa tataccttat cctgtaatct aaatcgaata tgtgtctgtt
    21481 gcttgcttcc aaaaaaacca tactgccgtg aattttgtca atagcgttgt ttgccatttc
    21541 aatcatcctt cttgtcttac cggtgccgct atgaccgcaa accaattgaa ccataataat
    21601 cacactccta ttcataattt tataatagta aagacactta tattgtacca catcttgtaa
    21661 ttgtttacac cattttattt acaattaaac aaatttattt ttactgcctt gatacctgtt
    21721 ttcagacttc attttataaa gaatatcatc atttattttc caccagctca taccccagtt
    21781 tacaaaaaga gaatctttct cgggtgccct tcctatcaat ctttgtactg taatatccac
    21841 atctaaatgc tccagaaaaa caattactct ttctacatat tcatcctttg aaatgagtgt
    21901 tatttcattg tttttatatg ctcttcccat ttcggtattt tccattatat aaagagaatg
    21961 aattttaacc tggttaattt ttaaaacaga taatattttt gccgtttcaa ccgtgtccaa
    22021 aatggtatct ccgggtaaat tcaatataat atgtgtacat atttcaaagt tatacctctt
    22081 aatccttaat acggcatcaa taaattccgc taaggtatga cctctgttaa tatttttcaa
    22141 agtatgatag tttactgtct gaagtcctaa ttcgattgtt atatttacat tttgtttttt
    22201 tatttcgtta aggaactcaa gtatttcatc agatatgcag tcaggtctgg tggatatggc
    22261 tatttccact atattatcca ttattgcatc atttatataa ttcttaaacc tttctatatc
    22321 catatatgta ttcgtataat tttgaaagta ggcaataaat ttcccactct tatatttttt
    22381 tgaaatatag tccatatttt tatttaactg ttcttttacg cttaaagaat tatcaagcat
    22441 ttcaaaaccg gttcccacgt cggcacaaaa tgtacaacct ccgtaaccca gagttccgtc
    22501 tctgttagga caggtacatg gaatatttac gggaagttta tatacttttt cgccatactt
    22561 tagttttaaa taatcagaat acttattata caatttcata tttttccctt tttataataa
    22621 tttttcttta atcattataa ttaagttaat agattaaatc aattaaatta ccagtaatta
    22681 ataaaattat tttacgatta aatgtaaata atattaatac aaatttaaat aaaggaggct
    22741 tcatatgcgt ataaaagaag gtatcgtccc gactattttg ggtacagttg ttactgcaac
    22801 aggagttgct ttaaaaaaca caaacctgcc ggaatcttac agaaacggat taatcggatt
    22861 tggattagcg aatattgcta taggttcagc tgaattagtt tcaagcaatt ggcagcacag
    22921 ccgcataatg aacagaatga caaaaatggc acccttcaag taagcaggtg tcccaaatct
    22981 ttgatttggt gacagtcgct tatcctgagc acaagcgaag gatctcagat gccccaaatc
    23041 tgtgatttgg cggcagtcgc ttatcctgag cacaagcgaa ggatctcaga tgcccaaaaa
    23101 aacaaaacat gccgcgtgag catgtttttg ttttttgtct gcccttattc aagcaaaaat
    23161 cacatacaat aatgaatata agccggctgc accaattaat agataaataa tcctgccaaa
    23221 tttacttttc cagcccccaa acaaattttc cactatatca atttttgata gcccgtacaa
    23281 cccccagttt aaagctccta taattataag aattgaagct attgcagtaa ttattctcat
    23341 agttcctcct caatattgca taatattaat acattatatt ttaggagtta tgaaaatatg
    23401 tacaaagctt aaatattttt atttaatttt gaatatttct gacaattcat aatacactcc
    23461 ggattcatcg tttgaaaagc gcgaaataga atctgcagcc ttctttaatt cgtcggttgc
    23521 atttttcata gctattccca gacctgagtt tttaataagc tcaatatcat tattgtcgtc
    23581 tccgatggca attacttgat ttgcatctat tcccagtctg tcgatatatt tttttaatgc
    23641 acaccattta catccattaa tatttagcag ttccaataga gaccagttgc ttatgttttg
    23701 attgagtatt atattgaact tacctggtat tttttcaatt tcacttttaa gttttaataa
    23761 tttttgatat tcattgaagt agcaaaccga caaaatatta tttatttcct ctgtcttaaa
    23821 ctttttgcac cttgttcttt tataatcctt cttaatatat cctaagtata cttcttcgta
    23881 attttcacat tcgtaaataa tgtcatatcc ttgattgtat tcatcaacat gaattatcgg
    23941 atttaaatca tgctttaaac ctaattcata tattctctca aatgtagaaa aatccaaata
    24001 attgctttca ataaattcgt cattcacata atgccttaca accgcaccat tatttgctaa
    24061 aataaccggc tccgtaaatt taagcctttc tattaaagtt ttagccatat aataatttct
    24121 gcctgttgct ataacaatat gtatgcccct tttatgaagt tcgataagta tatgtgcatt
    24181 tttatcactt attgtttttt cgctatttag aagagtaccg tctaaatcca atactaccat
    24241 tttgtatttc attttagtct cctgctctgt actattttta attatactac agaaaacaaa
    24301 aaaaataaac agaatgatta cagcataatg atactgttca tcatcccgag cgtagcaaag
    24361 atctcataag attcttcgag ctaaagctct cagaatgaca ataatgttat tcataggatt
    24421 acaaaagccg ggatttctcc cggcttagtt aatgcttatt atattatgtt attcttctac
    24481 ataagaatac ataaagtatt tatatcctaa tgggcttgca aaagcacctt gaagatctgt
    24541 gctcttcatg aaaatatctg tatagtagta aattgggcaa atagctcctg tttccatcaa
    24601 tatattttct gcttcatgca ttaaagcaaa tctttcttca gaatctttag aagcttttac
    24661 ttcagtaatt atcttatcat atgattctgc ccaagttttg ccgccttctc ccttgtaagt
    24721 gtaacctgca tatgttgcat gctcgccctt gccaaattgt gaatcgttgt taccgcttga
    24781 tgtaatccac atgtcaagga atgatatagg atcgttgtag tctcctaacc agccgtttct
    24841 tgcaaccgaa tagtccccat ttttacgagt gttaaggaag gttgcccatt cttgtttttc
    24901 aattgtcatg ttaattccat attgtgcaaa agtagcttgc agatttatgg caattgcttc
    24961 atggcctgtt ccttcatttg ttaaataagc aattgtcggg aattcttttg aaactacgcc
    25021 attttcatcc acagtgtaca atcctgttga ttctgcaact tctcttaaca aagctacagc
    25081 ttcttcgcaa ttcttttcat aatcatcagg gtcaacgctg taatatcctg ctccgtctcc
    25141 tttaggacca tttttagcta tatattctgt ttttccatcc ggctctgtta atcccatagg
    25201 tacaaaacct gctgccggta cctgacctgc ctggccgatt tcttcaacta tataagttct
    25261 gtcaatcata agaccaagag cttttcttat tttcgctttg tcagcttcac cgaaagcttt
    25321 gaaattctca tcatttacgt tgaaagaaac atagtatgtt ccaagttgtc cttctataaa
    25381 gaattcatca ggatactgct cctgcaatga tttaatttca tcatttggaa cttggtcgat
    25441 gaacaagtat gagccgttct ggaagtttgc taaagttgaa gtatcatcat cattaaatgc
    25501 aaatactaat tcatttgtaa ttacgcggtc tgcatcatga taatattcgt tcttctgcat
    25561 tatcaactgg ctttgtgaga attcaacaac cttgtatggt ccgttcccaa tatatgtatc
    25621 agctgctgta gcccaagcat catttccttc ggcaacttct gcttttacag gcatatatgt
    25681 cgggaatgca actaattcat tgaagtaggg cacatctacg ggaagaacaa cagtcaatgt
    25741 ttttccatct tcagatgctg taacattaag ctttgcatca ggatttacaa gatttccttc
    25801 atcatccatt tctgtcattt ctgcataacc gtctacaact tcaaacatat atccatagtc
    25861 tgctgctgtt tccgggttag ctgctctgtt ccaagcataa acgaagtcat gagccgttaa
    25921 gtctgagccg tcgctccatt tcaagccgtc ttttaactcg tatacatatt caacctttcc
    25981 gtcgtctctc tctttgccca caggtaattc tttagctaaa tcaggataaa gttcaagaga
    26041 tccgtcttct ttttgttgat agcctactaa tcctgcaaat gcgtggatta tataagtagc
    26101 accgtcaact gcactgttaa gtgccgggtc aatagtatca ggatatgcac caacatgcaa
    26161 agtcaaacta tctgcaatac ctggttcttc cggagtttcc ggagtttcag gggtttctgg
    26221 tgtttccggt tctgccggtg gttgattatt tccgcatgct gctaatgaaa acaccatggc
    26281 caatactaag attaaagata aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnggtacat caccttcaag taaaatcctc tgatggcttc ttgcatagct cgggtcagga
    26461 ataggtactg ccgacattaa cgaaattgta tatggatgaa gcgggtgatt atacaattca
    26521 tcggaaggcg ctaattccat tattctacct aaatacataa ctgcaattct attggatata
    26581 tgttttacaa ccaataaatc atgagcgata aagagataag tcagtcccaa ttgttcttgt
    26641 aaatcttcaa atttattgat aatttgcgct tgaattgaaa catccagagc agatacgggc
    26701 tcatcacaca ctataaattc aggctcaaca gccaaggctc ttgcaatacc tattctttga
    26761 cgctgccctc cagaaaattc atgagcataa cgagtagcat gctcgcttga cagtcctaca
    26821 atttccatta attcatttac tcttttgtct ctttcttttc tgctcttata catattcata
    26881 acatccaagg gctctcccac aatatccccg actgtcattc tcgggtttag tgaagaatat
    26941 ggatcctgaa ataccatttg tgcattttgt cttaattgtt ttatagactc tttactttca
    27001 atctttttgc ctttaaaaat tatttctcct ccagttggtt tgtacaactg caatatagac
    27061 cttcccactg ttgttttgcc gcaaccggat tcaccaacca aacctaaggt ttcgcctttt
    27121 ctaatactga atgagacatc atcaactgct tttaaaggtt tgctctttaa caagccgacc
    27181 ggaatatcga aatactgttt taaatttcta acttcaagaa gattgttgtt attttccatg
    27241 atacatctcc tctacttgtt ttattgtaag agtttcattt tcaattcctt ctcttacatt
    27301 catccagcac ctggaagtat gtttgccttc tattgtcatc tcaacgggat ttacagtaag
    27361 acaaattttc attgcattgt cacatcttgg tgcaaatgga catcctgccg gcatgtttaa
    27421 taaatctacc ggattgccgc cgattggctt aaggcgctgt ttttcatctt gtaccgtagg
    27481 tatggatttt aacaatccct tagtatattc atgctttgga ttgtaaaaaa tatcgtctgc
    27541 tgttcctctt tccgatattt gtccggcata cattacgata atatcatcac acatttctgc
    27601 cactacacct aagtcatggg taatcaggat gattgccata cctaatttct tttgcagctc
    27661 tttcatcaaa tcaagtattt gtgcctgaat ggtaacatct aaagccgttg taggctcatc
    27721 tgctatcaaa atatcaggtt cacaggcaag agccattgca atcataactc tttgtctcat
    27781 acctcccgaa aattcgtgag gatattgctc tattcttttt tccggttcat ttacaccgac
    27841 caatttaagc atttcaattg ctctagcttt agcttcttct ttatttctgt ttgtatgcaa
    27901 taaaattgct tccattaact gatttcctat agtaaataca ggattcaagc ttgtcatagg
    27961 gtcctggaaa ataatactta tttggttccc tcttattttc tgcatagttt ctttgctgca
    28021 gccaaccaat tcctgtcctt ttaattttat gctgccgctt acaattcttc cgggatttgt
    28081 taaaatttga agtattgaat atgctgtaac gctcttccca gagcctgatt ctccaacaat
    28141 acccagcact ttgccttctt ctaagttaaa tgatactccg ttaacagcct tcacctcgcc
    28201 ggaagaagtg aaaaatgaag tatacaaatc ttttacttgt aataaactca tttcattcct
    28261 cctacttctt taattttggg tcaaatgcat ctctaagccc gtcacccaac aggttaaagg
    28321 acaatactat caaccaaata gctattgcgg gtattattaa tctgtatgga taagagtaaa
    28381 tcccggtcaa tgcatccgaa gctaatgaac ctagactggg catgggtatt gaaacaccca
    28441 aaccgagaaa actcaagtag ctttccgtaa atatagctga cggaatctgc aatgctgtag
    28501 aaataattat aacactcata ctgtttggaa gcaaatgctt ctttataatt ctccatggac
    28561 tggagccaac tgattttgct gctaatatat attcattttg cttaattgat aaaatttgac
    28621 cgcgcacaag tcttgccatg ctaacccaat acagaagagc aaatactata aatatactta
    28681 tcatatccgt acccactttt gcaagtgaag taccttctat cttaggctgc aatacttgat
    28741 ttaaaacaac cgataacaaa atgataataa gcatgtccgg caaagaataa ataatatcaa
    28801 caatcctcat catgaaaagg tctacttttc ctccgaagta acctgcaata gagccgtaaa
    28861 ccactcctat tatcaatacg attattgctg cgaaaaatcc aaccagcaag gaaattcttc
    28921 cgccgtatat aactcttatt gcataatctc ttcctgcagc atcagttccg aatatatgag
    28981 gaaatatttt ttcaccgttt gcaatacgct ccaattctgt ctctccatat tcaaagggtt
    29041 ttaaattatt ataagtttta tcgattcttt ttccttgtgc cactcctaat tgctgctcat
    29101 aggtataggg atacaccata ggtacgaaag tcactattaa agctatcaga atcaatacag
    29161 tcaagcatgt cattgctatt ttgttctttc ttagccttct catcccatcc ttaaagaagg
    29221 tgatactttc gctcatcgtt acctgctgag ctttttcatc ttctgttgct ttttcaaaat
    29281 cctttaaatt aagttgaaaa ctaagaggat tctttttagg catttcatct atattattca
    29341 tatgtcacat cctctctttc tatcccaatt ctattctcgg gtcaaatact ttgtacaaaa
    29401 tatccgaaat aagcatcatt ataattataa gagtacctaa aaatattgtg gtccccataa
    29461 tcatagtgta gtcacgattt ataatgcttg aaacaaattc ttttccaaga cccggtactg
    29521 caaatatttt ttcgacaacc aaacttcccg taataatata tgcaaacatt ggaccggcat
    29581 atgttacaac aggtgttacg gcatttcgga gagcgtgttt aaatattacc tttgcaggtt
    29641 tcactccttt tgcccttgcc gttcttatat aatcctgact taatacatca agcatactgg
    29701 acctggtaag tctggtagtg tatgacatgg gataaaggct gagagatata ataggaagta
    29761 ttagtccgcc gggctcattc ccattggccg gaaaaattcc catccacaca caaaagacca
    29821 gtaaaagcaa tgttgctata ataaatgacg gcatggcgac caggccggtt gtaacaacca
    29881 taattatttt atctatagtc ttattatgaa agatagccgc aactgagcct aatacagttc
    29941 ctatacaaag agctatcacc gcagcagttc ctcctaattt aaaacttact ttaaagccct
    30001 gcataataac tgctgttact tctcttcctc tttgtttaat cgaaggaccg aagtcacctt
    30061 tcataatacc ctttaagtat gttacatact gttcagactt aggtttgtcc aatccgtatt
    30121 ttgcttccag cgccgccgta acagccgcac ttggtgcttt ctcgctcaag aaaggacctc
    30181 ccggaacttc atgcattacg aaaaaagtaa tggtgattat taaaaatatt gttatcactg
    30241 ccaaggtcag tctcttaaca atatataacg ctaattttga atccattatt acctccaaca
    30301 ttttaaatat aatgaggatg ctaataaagc tccctcagag tcatctcaat tctattcaaa
    30361 acacaaataa ttataccata agatttttcc tatatcaata tatttatttt tatgcctgaa
    30421 ttgtatttat atgccaggaa aataatatta aaaccataat atgcataagt ttagtaattc
    30481 taatatacaa aaaattatat taaaagaata tgttatcaat ataaaaaaac gacaacatat
    30541 tccttgttat aactatttta ttctctttaa tatttcacat aataaattat atatattttc
    30601 tacagagctt atgcttacat gctcgttagg agaatgcgca tcatatgcat caggtcccat
    30661 tgatattgca tcaagtcccg gaattttatc catcagaagt ccgcattcca gacctgcatg
    30721 tattgcgcct acaaggggct ccttattaaa catatcctta taagcctcaa tgcaaatatc
    30781 tctaatatta gattcctttg catattccca gcccggataa taacttccca
//
LOCUS       DAOSHP010000014        26330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1461_length_26330_cov_9.554900, whole genome shotgun sequence.
ACCESSION   DAOSHP010000014 DAOSHP010000000
VERSION     DAOSHP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 26330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26330
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1461_length_26330_cov_9.554900"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..547)
                     /locus_tag="PLB33_02710"
     CDS             complement(269..547)
                     /locus_tag="PLB33_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOG62464.1"
                     /translation="MNKAELIASVAEKTGFTKKDAEKALNGFMETIKEELVAGGKVQL
                     VGFGTFEVRDRKERQGRNPRNPGETIDIPASKAPVFKAGKSLKEAVNK"
     gene            complement(597..2030)
                     /gene="mazG"
                     /locus_tag="PLB33_02715"
     CDS             complement(597..2030)
                     /gene="mazG"
                     /locus_tag="PLB33_02715"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOG62465.1"
                     /translation="MSIIQIIGLGAGGEDDLTVRAQKALSEKIPTFARTDRHTIVNEL
                     RKNIDIVSFDDYFEKYETFNEVYENIVNTLIEKSKQYGKINYCTAGSPYYGDIVTKKL
                     INEYKKQINIIIIDGMSFLDKCLKLSGYADYNNIKILDCLEADEFSFDVNSFNIITQV
                     YDLDIASVLKIKLMEIYPEDANSVIIDVLEEKIEKVPLFMLDQEKNYGFSTYFCILPI
                     ELSRNRVYNVDNLLRIVKLLRGPDGCPWDKKQTHHSIRQHVIEEAYEVVDAIDNDDVD
                     NLVEELGDLLFQVVFHAELGSEKGYFNFSDVVTNVCKKMYFRHPHVFGDVKADDVEEA
                     LTSWEHSKLKEKNLTTYTDNLKNVPKALSPLSRSYKIQKRAAEVGFDWSDAKGAVLKI
                     KEELLEFIDEYNKGNIENMEEEFGDLLFALVNFARFEKINPDIALNKTINKFIERFEY
                     IEKNSTKDLKQMTLNEMDELWEESKFR"
     gene            complement(2032..3624)
                     /locus_tag="PLB33_02720"
     CDS             complement(2032..3624)
                     /locus_tag="PLB33_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG62466.1"
                     /translation="MSKESFIKGAAILSVAGLFVKILGAVYRIPLTNLIGTEGIGHYQ
                     PAYNIYNLLLVVSLSGFPTAIAKMVSERRALNNYKGAYQVYKIAGWGLFLIGLVSSIF
                     VLIFARNLVTFLGFPGSYYSMLALVPALFAVPLLSAYRGFFQGMQNMTPIALSQLIEQ
                     IFRVAVGLYLAYALAGTGLEEAAAGATFGASAGGIAALILIYIMFIFSKKSIKEEISV
                     SKNNEAEPVQYVIKSLLYIAVPITIGASIAPLMGNMDSFIVSNRLAALGYTVEEYTDM
                     FGELSGTAQTLINFPQVFSTAVAMSLVPSITEAFTKKQGIRLNETANAGVKMSLIIGL
                     PCGIGLFMLAEPIIALLYPSIGPAKHASSGALLEILSIGVIFLTLVQAFTAILQSVNK
                     QFHPVKNLALGLIVKVIVSYMLIGIPNINIKGAAISTTIAYLFVALFNWHDINKTKIK
                     IDLIKVSSRALLSTAAMAVTVWISFSVILSAIGSRNLATLGSIAIAGTVYVICLFITG
                     AITKEDLELIPKGEKLKKFVRK"
     gene            3879..6107
                     /locus_tag="PLB33_02725"
     CDS             3879..6107
                     /locus_tag="PLB33_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOG62467.1"
                     /translation="MERLKGTIVETIFRNNENGYTVALLETKNELITITGVFGTDISG
                     ENIEVFGKRVHHPKYGEQFQVDIYNSILPTGLEQIENYLSSGLIKGIGRHTAKKIVEA
                     FKEATFDIIQNTPEKLTEIEGIGEKKADMIAKSFQEQADVKEIMMFLQSHNISTNYNI
                     KIYKEYGKNTINVIKENPYRLAEDIYGIGFKLADKIAASLGVEKDSPYRISSGVKYIL
                     AEYAARGHTFVPYNLLVKSSANLLETSIETIEDEIRNMAFTDKIHIETIDSETVVYYL
                     PYHTAEVNVCKNLINLAGFKTDISEEDVLMYLESDEDNSINLTEMQKEAVVQSILNGV
                     TVITGGPGTGKTTIILSIIKIFEAMNKKVLLCAPTGRAAKRITESTGREAKTIHRLLE
                     YAYGENDSNLVFNKNENSPLDCHAIIVDEMSMVDILLMNNLLKALKRGTKLIMVGDVD
                     QLPSVGAGNILSDIIKSNTIKTIRLDMIFRQSAGSMIVQNAHAVNKGMMPLLNIKNSD
                     FYFIRENNINNIAKLIVELYKERLPSKYGLNPMKDIQVLSPMKKGDAGVLELNKKIQE
                     AVNPPSKLKKEKVLGKYTFREGDKVMQIKNNYQTEWKLTGNNLVTGEGIYNGDIGNIL
                     FIDEAEQEMYVQFDDEKEVVYSFNQLDELILAYASTVHKSQGSEFPVIIMPIVWAPPM
                     LLTRNLLYTAITRAKRLVVLVGQEKYMEEMIENNKIDKRFSALDYRLKSAVETYKLLT
                     DL"
     gene            6181..6900
                     /locus_tag="PLB33_02730"
     CDS             6181..6900
                     /locus_tag="PLB33_02730"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOG62468.1"
                     /translation="MKGYVKSFLELLYPEKNTCFICNVYDSSIDDKYICSDCEKKLIK
                     IVPPLCIKCSKPIDYASDCLCPDCNVYEKSFEAAKSPFSYEGIIKECIYNFKYYNKPY
                     FYKFFGNLLLRYMNEINYTNFDFITSVPLHKSKMRTRGYNQSQLLAKYMSDKLSIPYA
                     DVLKRTVKTTKQSQQSKDERKKNLKGAFAVKYPHTNTKIKNRSVLLVDDVYTTGSTAD
                     ECSNTLLNFGVSKVYVITIAR"
     gene            complement(6884..7459)
                     /locus_tag="PLB33_02735"
     CDS             complement(6884..7459)
                     /locus_tag="PLB33_02735"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOG62469.1"
                     /translation="MESKSKIRKRILNVRNNMSKEDVKKNSNAIMDKITSLDIYKHSK
                     VVFIYMDFKNEVMTSNLIKRMLSEKKRVVIPYTDSINTVLIPSEITKESDLKQNSFGY
                     FEPKSILPVNIEEIDLVIVPGVVFDKNLNRIGFGKGYYDKILNRLKPSAKKVALAHDF
                     QVLEDIPAEEHDVKMDMIITEKNIYPFTGLL"
     gene            complement(7607..8215)
                     /locus_tag="PLB33_02740"
     CDS             complement(7607..8215)
                     /locus_tag="PLB33_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOG62470.1"
                     /translation="MNYNIKDILEIMPGFLLGISIHEFSHGLAADRLGDNTPRLQGRL
                     TLNPAKHLDPIGFLCLLVFGFGWAKPVMINPRNFKNPRRDDAIVSLAGPISNFLIAFL
                     FMGLIKLTINTSLSPTAIDVFYGTVMVNLMLMVFNLIPIPPLDGHHILGSIGGPKVWN
                     FYFKYREHLRFALLLLIIFRGIGRIIGPLIFILYRLLVSIFF"
     gene            complement(8242..9576)
                     /locus_tag="PLB33_02745"
     CDS             complement(8242..9576)
                     /locus_tag="PLB33_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOG62471.1"
                     /translation="MLEEGSLWGPLVLQFILLFINAIFASAEIAVISMNDNRIAKMML
                     EGDKRAIRLSKLTDNPSQFLSIIQVAITLAALLGGAFAAENFAGRLTGLLINIGVNIP
                     YSVLNSVSVVLITLLLSYITLVMGELVPKRIAMKNAERLALSLSGLLYFISKLFAPVV
                     WLLTVSTNGILRLLGIDPNETDDEVTEEDIRMMVDVGSEKGSINENEKEMIQNVFEFD
                     NKTAEEVMTHRTEAVILWLDETEEEWDKKISESRHSRYPVCDGSADNVIGVLHVKEYY
                     TIEDKSRENIMKMAVKPAYYVPETVRTDVLFQNMKKSRNHFAVVFDEYGGMSGIITMN
                     DLLEQIVGDIDDDYLTPEEEEIKKIDDRTWRIKGSAYLDDVSKELGVSLPDEDFDTFG
                     GLVFGILGIIPEDGSTLELEEYGLKIVITEIKGRRLETATVYKNVDNDEDTN"
     gene            9808..10386
                     /locus_tag="PLB33_02750"
     CDS             9808..10386
                     /locus_tag="PLB33_02750"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOG62472.1"
                     /translation="MFFEALILGIVIGYIRRGKLSRLSYVNFSFRFLIYISALFYLGI
                     IVINLGLYDYESFLYSTFLIGSIVLTGLFLIANISIKYMFIPLIGLALNLLSFLTNNF
                     KFPLSPDAAAKIYGAEAAELLNAGKLLFYTSSETATLSFLGNIIPIGNWFVVSIGDII
                     ASLGVVLIVQCIMADKFIQTRSKITFSKDIFR"
     gene            complement(10426..11385)
                     /locus_tag="PLB33_02755"
     CDS             complement(10426..11385)
                     /locus_tag="PLB33_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOG62473.1"
                     /translation="MLTIEKIKEAQKRIAPFVYKTPLLRLNNLDEYLGCQVYTKMESF
                     QKTNSFKVRGAVNKLMTLSEEELNKGIITVSSGNHGKAVAYAATLLGRKARVVIPDNA
                     PKIKVEGIQSYGAEIIYSIPSERYNVARQLSEENGWSLIPPFDDYEIIAGQGTAGLEI
                     LEQLPDADYIVVPIGGGGLIGGLSAAVKEINPKVKVIGVEPALVPRYSESFKAGHPVS
                     LDPNSKSVADALQTLRPGERNYPIIEKYVDKIVTVHEENILKATKFLLTIGKLLAEIS
                     SSITIGAVLQKAVEFEPEDKVVFLISSGNIGIDQFNKFENIDL"
     gene            complement(11422..12345)
                     /locus_tag="PLB33_02760"
     CDS             complement(11422..12345)
                     /locus_tag="PLB33_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG62474.1"
                     /translation="MEYIGLVLLLLIVVILFATNIRIVPQAHAYVIERLGAYQATWGV
                     GVHVKVPLIDRVVKKISLKEQVIDFPPQPVITKDNVTMQIDTVIFFQITDPKFYTYGV
                     ERPISAIDYLSATTLRNIIGDLELDETLTSREIINTKMRAVLDDATDPWGIKINRVEV
                     KNILPPKEIQNAMEKQMKAEREKREAILIAEGQKSSAILVAQGNKESMILQAEAEKQS
                     AILRAEAKKEATIREAEGEAEAILSVQKATAEGLKMIKDVELNKEVLTFKSFESFEKA
                     ANGQATKIIIPSEIQGLAGLSKALSETIKES"
     gene            complement(12356..12787)
                     /locus_tag="PLB33_02765"
     CDS             complement(12356..12787)
                     /locus_tag="PLB33_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG62475.1"
                     /translation="MEYIGYAWLGIVVACVAIEAMTVSLTTIWFAIGAIFAWLIYLTG
                     LGLEVQIVVFLLVSIVCLIFTRPIAMEKLKVGRVKTNAESLIGQYFKVESTIDNINNT
                     GTVNVKGQMWAARSLDDQVIEKDEIVIVKKIVGVKLIVEKK"
     gene            complement(12885..14444)
                     /locus_tag="PLB33_02770"
     CDS             complement(12885..14444)
                     /locus_tag="PLB33_02770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG62476.1"
                     /translation="MKKIIIIVILCVLFSGCSTFFSDVTEIEAVNLQNGVKIVLPKDS
                     KDADIIIDAVNKKNKTDEDITSLLSYEIILKKENDEEVYKLYFDLDNQTAYLAQDKTV
                     FKIRDNIAKQLFLSDAFSYIYISAMYNSYIEVNGQQINPDIEYNWNSRNIEGDFVNNA
                     GLLTGKYEENEKITVSHSDSIDIKHEKQPDSQIIRIYEQGSLFGTGKNLSELISNIKS
                     DGEYLVECESQWHFKENRESYGSQKLKFSISVDIPAQINIVTKENYPGNILLISIENL
                     NDDETVKLETDAVKTNFDLYAYKDKNYYMMPIDLYTQPGEHNVIAVVNEGAEDEYTLE
                     KTLTVSNRSFKTQYLTVSEEMNETHNDNAAIYEFAQLVKPARTESAKEKLWEGTFIMP
                     VEGELTTDFAEIRYVNNEISSSRHSGLDLAAPKGTPVVAPNNGRVTFAMEGLLSPGNT
                     VVIDHGMGLFTSYYHLDTINVKKGDEVKKGDVIGTVGTTGFSTGPHLHYAVSIYNTYV
                     NPYQTLSGIID"
     assembly_gap    14491..14590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14591..15541)
                     /locus_tag="PLB33_02775"
     CDS             complement(14591..15541)
                     /locus_tag="PLB33_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62477.1"
                     /translation="MFIKFLIAAVFAGTPLLFGTVGEIMNEKAGHLNLGVEGMMAMGA
                     CAGFMAGFFSNSIYIALLAAFLAGMLGALIYAVLTITFMANQNVTGLTLSIFGVGISN
                     FAGEFMLVNSASNSLKLPEQITAQISNINIPGISTIPVLGPLLFQYNIFVYLGVVIAV
                     TCGIYYSRTKTGLNVRAIGENPGAADAAGIKVTKLKYINVLIGGGICGIGGAYCSMII
                     NGGVWMNNPVNGQGWIAVALVIFASWKPLRAIFGSFIFGAFNILKYYFPKTIFTIPNA
                     FYDMLPFAITALVLVVTSIRKSKENQQPASCGVNYFREER"
     gene            complement(15543..16625)
                     /locus_tag="PLB33_02780"
     CDS             complement(15543..16625)
                     /locus_tag="PLB33_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62478.1"
                     /translation="MNNRSHTPFIRTVKISERSSNDILKIRIAAFVLALTAGGLFILL
                     LGFNPLKLYGTIISGAFRSKMAFQATVKFMIPLLMSSLGVSIAFKMRFWNIGAEGQII
                     VGAVCASYFALFHDNWPHFLLLIVMFAAGAIGGGLWGLIPTYFKSKYDTNETLFTLML
                     NYIALHIITYLRDGPWKDPGSSGFPKIARFTANAQIDKVLGVHFGWIIALILVVIVFI
                     YLKYTKHGYEISVVGESKSTARYAGMDVKKIILRTMIISGGICGITGMIQAAGSDMTL
                     ASSVAGGVGFTAIIVSWLAYLNPFGILLVSFLFSILDKGSSVMQSTYGLSTYFASVLQ
                     GIILFFILGCEFFIRYKFVLTKKGGK"
     gene            complement(16618..18150)
                     /locus_tag="PLB33_02785"
     CDS             complement(16618..18150)
                     /locus_tag="PLB33_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62479.1"
                     /translation="MLKTIDNYAIELKGITKSFGKVIANEDVNLGIKYGEILALLGEN
                     GSGKTTLMNILSGIYYADEGTIFIDGNEVFITNPHEAIKLGIGMIHQHFKLVEVFSAM
                     DNIVLGTKGRYVKNSALKEKILEMSNKFGLEIEPDKKVYNMSVSEKQTIEIMKVLYRG
                     AKILILDEPTSVLTPQETEKLFVILRRMKEQGNAIIIITHKLHEVLAISDRVAILRKG
                     KVVDTVNTRETNEKQLTELMVGRPISLEIKRPETSRKKTVLKVVDLSVVNEDGTIGLN
                     NINFKLKSGEILGVAGVAGSGQRELCETIAGLMHPKTGAVLYNKENIIGKTPDEIINL
                     GISMSFVPEDRLGMGLVGSMDMIDNMLLKSYKKNKGFIIDKKPSRQLAEELVDKLSIT
                     TPNVETPVRMLSGGNVQKVLLGREIESEPNVLITAYPVRGLDINSSYIIYDLLNEQKK
                     KNVAILFIGEDLDVLLELSDRIMVLCHGELMGLVDAKNTTKEEVGLMMAGRKMEGVDS
                     DE"
     gene            complement(18295..19458)
                     /locus_tag="PLB33_02790"
     CDS             complement(18295..19458)
                     /locus_tag="PLB33_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG62480.1"
                     /translation="MKKFLGIILALTMILSLFTACGSNGQQTKPESEGPEVSGEGIFP
                     AIAKEDIKVGVIHIGNPADGSGYTFAHDQGIIEMQKALGLSDSQIIRKNNVNDTDPTA
                     TDAAIRECIEEGAHIIFGTSWGYMDTMEALAEEYPDVIFSHGSGYKSNDRNFNNYFGR
                     IYQARYLTGIAAGLKTKTNKLGYVAAMGKDNSEVTGGLNAFAMGVHSVNPEAKVYVKV
                     TNSWYSPEEETNAAKALIAEGCDVIAQHCDTPNPQLEAEKAGVFGIGYNSDMSKDAPK
                     ATITSAVWNWGAYYTWAVEKVINGTWLSENYYGGMKEGMIDISPINESIAAEGTVEKI
                     EEAKQSIMDGTFNVFDGVIETNDGSTVGEEGKTLDDGTITGGINWYFKTVVEK"
     regulatory      complement(19526..19627)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(19776..19961)
                     /locus_tag="PLB33_02795"
     CDS             complement(19776..19961)
                     /locus_tag="PLB33_02795"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOG62481.1"
                     /translation="MPIIQVEMLKGRTVDQKREMVKEITEVVSRTANCPKEAVRIVIR
                     EMEFENFGISGKLQCDI"
     gene            complement(19994..21085)
                     /locus_tag="PLB33_02800"
     CDS             complement(19994..21085)
                     /locus_tag="PLB33_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOG62482.1"
                     /translation="MERYPMLEFNLKKLYNNTKIVTKLCEDNGIKVAGIIKGFGGIAE
                     GAEEMVKGGCYQIGSSRIEQLKALKDRGFDMPLLLVRIPMMCEIEDVVKYSDISLVSE
                     KETLNLLNKEAKAQNKIYGVVLMYDLGDLREGVFYKEELIRLAEYVEYESDNLYLEGI
                     GSNLSCYGSVAPTTENLSQLAEAATEMEEKLNRKLNIISGGGTTTLPLLVRGGVPKKI
                     NHLRIGEGINNTQDLPLYWDTNIEGLDPDVFVLKAQIVEINEKPTHPIGQLMVNAFGD
                     LPHYEDRGIRKRAIIALGNQDLGDSSRLVPKDKNMIVLGASSDHTILDIHDCDTEYKL
                     GDIIEFNILYQAMLMTTLSDYVHKKIIKY"
     gene            complement(21095..21547)
                     /gene="tadA"
                     /locus_tag="PLB33_02805"
     CDS             complement(21095..21547)
                     /gene="tadA"
                     /locus_tag="PLB33_02805"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOG62483.1"
                     /translation="MDEVFMEEALKEAYKAYEINEVPIGAVIVRKGEIVGRGFNQKET
                     LKDATLHSEITAIKDACKNLGGWRLPGCTMYVTLEPCPMCAGAIVNSRIERLVIGARD
                     LKTGACGSVLDITNNKKLNHQPEVKFGVLEKECTGILQDFFIKLRKKI"
     gene            21892..22911
                     /locus_tag="PLB33_02810"
     CDS             21892..22911
                     /locus_tag="PLB33_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62484.1"
                     /translation="MKTIKKLWALGLCGILVINMNSMAYADVLDDIIGSSTIEDREDA
                     ENTEDVYTDVTSEDVTGNDITVTDENIAKYSVLFSDIDNHWAKEWIKNAVGLGFVSGY
                     EDGTFKPDRTITRAEFSTMLNKAMKIEITENINFSDVREKEWFYKEIQKSVAAGFFSG
                     YENNTFRPNNPIKREEVAKVVAGAITTGNIDGEGATLLKDYSTIQEWAKDSVNTVYNK
                     GYILGYPDKTYMPSKALTRAEAVKIIFEIVDNENIEYGFNITNYNETYSSAVVVGNLN
                     VLDSVGNGNVYIKNVVVLGDIVISAKNVKSVELTDVKARRIIVEGENNPVRVVYNDNV
                     IIAKK"
     gene            complement(22956..24701)
                     /locus_tag="PLB33_02815"
     CDS             complement(22956..24701)
                     /locus_tag="PLB33_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62485.1"
                     /translation="MEKKNNFMPLLIYSIKEKKNWVLLSTAIIFLTTLLIPYILRVDE
                     EFFIMFGIVELFVLVLINCLVDNSFFHNESKLTYYSSKPVTLGRQIIINIVTNAVFTV
                     YLLALIVLSVIFQGLDYEVLKVFKMLIPWLSAIILLASLSSILSGNTLMAGAMTIFNF
                     CLPLIILLVIMFIFSILENLVLGFSADVLSDYFVNNIYRIDYLYFVRYVESKSIDLVY
                     LLLLPTILIFISLLINKFIKRRKNENTGFIVFNGFKYFVAVLACLIIPASFSIGFSRY
                     MSIGNRLIISALLAVLSYYIIIAFIEKSFRISKSSVKVFIVSMVAFAVITGGTVGVAS
                     QYENLVPEPEDVQMAYVGNQRWSINEIDRFLRGDYTSEKSDFPEWKRNRNIITYEDED
                     IIEIITELHEEVLKDQTFYHESDYYGINNFVIAYWMKDGSKIIRDYTLTATDKLTKEH
                     ETKNELANKLLNSIDYKKQKFYYIYDENYYSGRNLYADFKNISNLNNVIKSVYLNDLR
                     GALTKDIENLFIENDKAFIELSMNYDSRFNKAVMAGEQEYILEIYEKISDIDKNYINE
                     INLNKNFVNTLEMLK"
     gene            complement(24685..25575)
                     /locus_tag="PLB33_02820"
     CDS             complement(24685..25575)
                     /locus_tag="PLB33_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62486.1"
                     /translation="MLTINNLSKKFGDFQVLKNININAEKGRIYGLVGSNGCGKTTIL
                     KHIMKVYKQDEGHISFNGEEVVEGSDIIKEFYFVQDTLYFPYQYTLNKLFHYEKLMYN
                     MSEEKFEYLLKYFDIDKNKVLNKMSKGQKKQAAFVLSIAAQPRLLLLDEIVDGLDAVI
                     KRKFWDVLIKEIIDNNMTVVISSHDLKELDNICDRVGIMHEGRILREENLEKMKEDIK
                     RVQFAVEGEFDPDKLKEFNVLQVVKIGSVFIATLTGNIDELKKYLESKYTLLLFDKLS
                     MSLEEIFITELGGVGYGKEK"
     gene            complement(25562..25954)
                     /locus_tag="PLB33_02825"
     CDS             complement(25562..25954)
                     /locus_tag="PLB33_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG62487.1"
                     /translation="MIRIDNDNPKAIYEQIYDEIVRLIFSKSLKPDEKLPSVRELASM
                     IRINPNTIQKAYKYLEEDNYIYSIKGVGNFVKDADELRNLHLKNMKDELYKVVKSLKE
                     LGLRNERIIEMVRDILTPGTKGGHDVND"
     gene            complement(25969..>26330)
                     /locus_tag="PLB33_02830"
     CDS             complement(25969..>26330)
                     /locus_tag="PLB33_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62488.1"
                     /translation="INSGVFKMHPETKCKIYKFEVTDEDYDIITKRLNDFLAKPHKYR
                     YSFLNVFLIRFNIPYQREYHFVCSSFVSYLLKGIIPFNKEISLIIPDDYNNFNLIPEY
                     EGRLFEYVSKKVACVNA"
BASE COUNT         8156 a         5161 c         3955 g         8958 t
ORIGIN      
        1 cacatatttc ctccggttaa aatatagtat gattatatat gtgcacctat ttatttgtca
       61 atactacaat aataacccag gcgaagaccg tcatgcttaa tattcaaaag atgccctaac
      121 cctatatata aggtatgggg acacacctcc ctattcaggg atagcttctg atgttaagat
      181 gaatgtcccc aaatttaata tagggttagg gcatacaaaa gtttgtcaat agaatagggg
      241 atatcttcga tatcccctta atttgtgtct atttattaac tgcttctttt aatgattttc
      301 ctgctttgaa caccggtgct tttgatgccg gaatatctat tgtttcgcca ggatttcttg
      361 gatttcttcc ttgtctttct tttctgtctc ttacttcaaa agttccaaac ccaactaatt
      421 gtacctttcc gcctgctacc agttcttctt ttattgtttc cataaatccg tttaatgctt
      481 tttctgcatc tttctttgtg aaaccggttt tttcagcaac actggcaatt aattcagctt
      541 tattcataaa aatcctcctt aataaaaatg taatttattt tactttatct aaattcctat
      601 cggaactttg attcttccca caactcatcc atttcgttca aagtcatttg ctttaaatcc
      661 ttggtcgaat ttttctctat gtactcaaaa cgctcaataa acttattgat tgttttattt
      721 agagcaatat caggattaat tttctcaaac cttgcaaaat taacaagtgc aaacaacaag
      781 tcgccgaact cttcttccat attttctata ttgcctttat tgtattcatc aatgaattcc
      841 aagagttctt ctttaatttt caataccgcc ccttttgcat cagaccaatc gaatccaact
      901 tccgcagctc tcttttgaat tttgtaactt ctgcttaaag gtgataatgc cttggggaca
      961 ttcttcaaat tatctgtata tgttgttaaa ttcttctcct tcagcttgga atgctcccag
     1021 cttgttaatg cttcttctac atcatcggcc ttcacatctc caaatacatg gggatgtctg
     1081 aaatacatct ttttacagac attggttaca acatcgctaa agttaaaata acctttttca
     1141 ctgcccaact cggcatgaaa tacaacctgg aacaataaat cccccagttc ctcaactaaa
     1201 ttgtctacat catcattatc aattgcatcc acaacttcat aagcttcttc aataacatgc
     1261 tgtcttattg aatgatgtgt ctgttttttg tcccatgggc acccatcggg acccctcaat
     1321 aattttacaa ttcttaacag attatctaca ttatacactc tatttctaga taattcaata
     1381 ggcagaatgc aaaaatatgt tgaaaatcca taattttttt cttgatcgag cataaaaagc
     1441 ggtacttttt ctattttttc ttccaataca tcaataatga cggaatttgc atcttcagga
     1501 tagatttcca taagtttgat tttaagaacg gatgcaatgt ccaaatcata aacttgagta
     1561 ataatattaa aagaattaac atcaaatgag aattcatctg cttcaagaca atccaaaatt
     1621 ttaatattat tataatcggc atatcccgaa agttttaaac atttgtcaag aaaactcatt
     1681 ccatcaatta ttattatatt aatttgcttt ttatattcat taatcagctt ttttgttact
     1741 atatcgccgt aataagggct tcctgcagta cagtagttaa ttttcccgta ttgttttgac
     1801 ttctctataa gcgtatttac tatattctcg taaacttcgt taaaggtttc atatttttcg
     1861 aaataatcat caaaacttac aatatctata ttttttctta attcattcac tatagtgtgc
     1921 ctatcagttc ttgcaaaagt aggtattttt tctgataaag ccttttgagc tcttacagtt
     1981 aagtcatctt caccgcctgc tcccaatccg attatttgaa ttatgctcat attatttcct
     2041 cacaaatttt tttagttttt ctccttttgg tatcaattca aggtcttcct ttgtaattgc
     2101 tccggtaata aacaaacaaa tcacatatac tgttcccgct atagctatgg agcctaatgt
     2161 tgctaagttt ctacttccta tagctgataa tataacactg aaggatatcc atacagtcac
     2221 tgccattgcc gcagtggata aaagtgctct tgaagaaact ttaattaaat ctatttttat
     2281 tttagtttta tttatatcat gccagttaaa cagagcaaca aataaatatg caatagttgt
     2341 gcttattgct gctcctttaa tattaatatt tggaatgccg ataagcatgt aggatacaat
     2401 aactttaaca atcaagccca atgctaagtt ttttacagga tgaaattgtt tgtttacaga
     2461 ctgaagtatt gctgtaaatg cttgaaccaa agttaagaaa atcactccta ttgacagtat
     2521 ttccaaaagt gctccggaac ttgcatgctt tgcaggtcca atagacggat aaagcagagc
     2581 aattataggc tcagcaagca taaataagcc tataccgcat ggaagtccta taattaatga
     2641 catttttacg ccggcattag cagtttcatt taaccttata ccttgttttt tcgtaaatgc
     2701 ttcagtaatt gaaggaacta agctcattgc cacggctgtt gaaaaaacct gggggaaatt
     2761 tattaaggtt tgagctgttc cgcttaattc accaaacatg tctgtatatt cttcaacagt
     2821 gtatcctaga gctgcaagtc gatttgaaac tataaatgaa tccatgtttc ccataagcgg
     2881 tgcaattgaa gcacctatgg ttatgggaac tgcaatgtat aaaagacttt taattacata
     2941 ttgcaccggt tctgcttcat tattctttga tacagaaatt tcttctttta tacttttttt
     3001 gctgaagata aacataatat aaatcaatat aagagctgca atacctcctg cggatgcacc
     3061 gaaggtagca cctgcggcag cttcttcaag tcctgtacct gccaatgcat atgccaggta
     3121 taaaccaact gcaactctaa aaatttgctc aatgagctgt gacaaggcaa tgggagtcat
     3181 gttctgcatt ccttggaaga atcctctgta ggcggatagc aaaggtactg caaacaatgc
     3241 aggaaccagt gccaacattg aatagtatga gccgggaaaa ccaagaaacg taacaagatt
     3301 acgggcaaat ataagcacaa aaatagatga aacaagtcct attaaaaata aaccccaacc
     3361 tgctatttta taaacctgat atgctccttt gtaattattt agagctcttc tttcggaaac
     3421 catcttagca attgctgtgg ggaatccgga cagtgacaca acaagcaaaa gattatatat
     3481 gttataagcc ggttgatagt gacctattcc ttcagtacct attaaatttg tcagaggtat
     3541 tctgtagact gcccctaata tttttacaaa taatcccgct acgctcagta tagcagcacc
     3601 tttaataaaa ctttccttag acatgtttct tcctttctta caacaatata ttttggacat
     3661 tccttcttcc cgcagagact atcccggtgt gtccccatac ctttttcgct gaaatttagt
     3721 ttatcacagt ttattcttct tttaaagaga aaaatgcgaa tcagcagata tttttattat
     3781 ttttagcttc tttaaatagc aactttttat accttgtaac tatttttatg gtataatttt
     3841 attattacag ttcgggggaa ttccttaagg agaatttgat ggaaagactg aagggtacaa
     3901 tagttgaaac tatttttaga aataatgaaa acggatatac ggttgctttg ttggaaacaa
     3961 aaaatgaatt aataaccata acaggagtat ttggtaccga tatatccggc gaaaatattg
     4021 aagtgtttgg aaaaagagtt caccatccaa aatatggtga gcaatttcaa gttgacatat
     4081 ataattccat acttcccacc ggcttggagc aaatagaaaa ctatctttcc tcaggtttaa
     4141 taaaaggtat aggcagacat accgcaaaaa aaattgtaga agcatttaaa gaagcaacct
     4201 ttgacattat tcaaaacact ccggaaaaat taactgaaat agaaggaatc ggcgagaaaa
     4261 aagccgatat gattgcaaaa agctttcagg agcaggcaga tgtcaaagaa attatgatgt
     4321 ttctgcaaag ccacaatatc agcactaact acaacataaa aatttataag gaatatggta
     4381 aaaatactat aaatgtaata aaagaaaatc catacaggtt agctgaagat atttatggga
     4441 taggttttaa gttagccgat aaaatagctg catccttagg agttgagaag gactcgccct
     4501 acagaatatc atcgggagtt aagtatatac tggcagaata tgcagcaaga ggtcacactt
     4561 ttgtacctta caatttattg gtaaaaagct ctgccaattt acttgaaact tcaattgaaa
     4621 ctatagaaga tgaaataagg aatatggcat ttaccgataa aatacatata gaaacaattg
     4681 actctgaaac cgtggtatat tatctgccct atcacaccgc tgaagtaaat gtatgtaaaa
     4741 atttaataaa tttagccggc ttcaaaactg atatttctga agaagatgtt cttatgtatt
     4801 tggaatctga tgaagataat tcaattaatc tcaccgagat gcagaaggaa gcagttgtgc
     4861 aatccatatt aaacggtgtt acggtaataa ccggaggacc cggaaccggt aaaacaacaa
     4921 ttatattatc aatcattaaa atatttgaag caatgaataa aaaagtactc ttatgcgctc
     4981 caaccggaag agcagcaaaa cgcataacag aatcgacggg aagagaggca aaaaccattc
     5041 atcgcctctt agaatacgct tatggcgaaa atgacagcaa tttagtcttt aataaaaatg
     5101 aaaattcacc cctggattgc cacgctatta tagttgatga aatgtccatg gttgatatac
     5161 tgctgatgaa caatttactc aaggcattaa aaaggggaac aaagcttata atggttggag
     5221 acgtagacca attgccgtct gttggagcag gcaatatttt atccgacatt attaaaagca
     5281 ataccatcaa aacaatcaga cttgatatga ttttcagaca gtctgcagga agtatgatcg
     5341 ttcagaacgc acatgccgta aacaagggaa tgatgccttt attaaacata aaaaactcag
     5401 atttttattt tataagagaa aacaatataa ataatattgc caagcttata gttgaactgt
     5461 ataaagagcg tctgccttca aagtacggtt tgaatcccat gaaagatata caggtgcttt
     5521 ctcccatgaa aaaaggcgat gcaggtgttt tagaattaaa caaaaaaatt caggaagcag
     5581 ttaatcctcc ttccaagtta aaaaaggaaa aggtattagg caaatataca ttccgcgaag
     5641 gcgataaggt aatgcagata aaaaacaact accaaacaga atggaaattg acaggtaata
     5701 accttgtcac cggagaaggg atatataacg gtgatatagg aaatatatta tttattgatg
     5761 aagcagagca agaaatgtat gttcaatttg atgatgaaaa agaagtggtg tattctttta
     5821 accaattaga tgaattaata cttgcctatg catcaacggt acataaaagt caaggaagcg
     5881 aattccccgt tattataatg cccatagtat gggcaccgcc aatgctgcta acgagaaatt
     5941 tattatatac tgccataaca agagcaaaac gattggtcgt tcttgtaggt caagaaaaat
     6001 acatggaaga aatgattgaa aacaataaaa tagataagcg tttttcagcc cttgattaca
     6061 ggctgaaatc tgcagtggaa acttataagc ttctgacaga tttataattg gggatattcc
     6121 tctaaccaat aagactatac cggtacaaga ggtgtgtacc cataccaatt ggggggtaaa
     6181 atgaaaggat atgttaaatc gtttttagaa cttttatatc ctgaaaaaaa tacttgcttt
     6241 atatgtaatg tttatgacag ctccatcgat gacaaatata tttgcagtga ctgcgaaaag
     6301 aagctgataa aaattgtacc tcctctatgc attaagtgct ccaagcccat tgactatgca
     6361 tctgattgtc tttgccccga ctgcaatgta tatgaaaaaa gctttgaagc cgctaagtct
     6421 cccttctcat atgaaggtat tattaaagag tgtatctaca acttcaaata ttacaataag
     6481 ccctattttt ataagttttt tggcaacctc ctgttaaggt atatgaatga aattaattac
     6541 accaactttg attttataac atcggtaccc ctacataagt ctaaaatgcg cacacgtggc
     6601 tacaatcaat cgcagctgct tgcgaaatat atgagtgaca agctttcaat tccttatgca
     6661 gacgttttga aaaggactgt aaaaaccact aagcaaagcc aacaaagcaa ggatgaaagg
     6721 aaaaagaact tgaagggtgc atttgccgta aaatatcctc atacaaacac aaaaattaaa
     6781 aatcgcagtg tgcttttggt agatgatgtc tacacaacag gctccactgc cgatgaatgc
     6841 tctaatacac ttcttaattt cggtgtatca aaagtctatg taattacaat agcccggtaa
     6901 aagggtaaat atttttttcg gttattatca tatccatttt cacatcgtgc tcctctgcag
     6961 gtatatcttc aagcacttgg aaatcatggg ctagggctac cttttttgct gacggtttta
     7021 acctgtttaa aattttgtca taataaccct taccaaatcc gattctgttt aaattcttat
     7081 caaatacaac tccgggtaca ataaccaagt caatttcttc tatgttcacc ggtagaatac
     7141 ttttcggctc aaaataaccg aaagaatttt gctttaaatc tgattccttg gttatttcag
     7201 aaggtattaa gacagtatta atactatccg tatatggaat aacaacacgc tttttttcgg
     7261 aaagcatacg tttaataaga tttgatgtca ttacttcatt tttaaaatcc atgtatatga
     7321 aaacaacctt actatgttta taaatatcca ggcttgttat tttatccatt atcgcattgc
     7381 tgtttttttt aacatcttct ttagacatat tatttcttac attgagtatt ctttttctta
     7441 ttttactttt gctttccatt atatcaccaa ctcataaagt ttagtcatta gcaggctcca
     7501 tcaccaactt gtgcactccc tttggtcgtg caaattggga ttgcgctgca tatgatcgat
     7561 ctaacgctat ttttttctcc ctttagtcta aaatagggtt agggcattaa aaaaatatag
     7621 aaactaataa tctgtacaat ataaaaataa gaggacctat tattcttcct attcccctga
     7681 atataatcaa cagaagcaat gcaaatctta agtgctcacg atacttgaaa taaaaattcc
     7741 aaaccttcgg tcctcctatg cttcccaata tatgatgccc gtctaaggga ggtatgggga
     7801 ttaggttaaa taccattaac ataagattta ccattacggt tccatagaaa acatcaattg
     7861 cggttgggct aagtgatgtg ttaatggtaa gttttattaa ccccataaac aaaaatgcaa
     7921 ttaaaaaatt cgatataggg cctgccaatg acacgattgc atcatctcgt cttggatttt
     7981 tgaaatttct cggattaatc attacaggct tagcccatcc gaatccaaac acaagtaaac
     8041 ataaaaaacc tattgggtct aaatgctttg ccggattcaa ggttagtcgt ccctgtaatc
     8101 gcggtgtgtt atcgcccaat ctgtctgctg ctaagccatg cgaaaattcg tgtattgaaa
     8161 ttcccagcaa gaaacccggc attatttcta atatatcttt tatgttataa ttcattgtcc
     8221 ctccttatca gtatacactg attagttcgt atcttcatca ttgtctacat tcttgtatac
     8281 tgttgctgtt tcaagtcttc tgcccttaat ttccgttatg acgattttaa ggccgtattc
     8341 ctctaattcc aatgtgcttc cgtcttccgg aataattccc aatataccga aaaccaatcc
     8401 tccgaatgta tcaaaatctt catcgggcaa acttactcct aattccttag acacatcgtc
     8461 caaatatgcc gagcctttaa ttctccatgt tctatcatct atttttttaa tttcctcctc
     8521 ttcgggagtt aagtaatcat cgtctatgtc ccctactatt tgctccaata aatcattcat
     8581 ggtaataata ccgctcattc ctccgtattc atcaaaaaca acggcaaaat gatttctgct
     8641 ctttttcata ttttggaaca aaacatctgt cctcactgtt tcaggtacat agtaagccgg
     8701 ctttacagcc attttcataa tattttctct gcttttatcc tcaattgtgt aatattcctt
     8761 aacatgcaaa acgccgataa cattatctgc gcttccgtca catacaggat atcttgagtg
     8821 cctgctttca gatatttttt tatcccattc ttcctctgtt tcatccagcc acaatattac
     8881 tgcttctgtt ctgtgagtca ttacttcttc tgctgtttta ttgtcaaatt caaaaacatt
     8941 ttgaatcatt tctttttcat tttcattaat tgagcctttt tcacttccta catcaaccat
     9001 catccgaata tcttcttctg ttacttcatc atcagtttca ttggggtcaa tccccaataa
     9061 tctcaatata ccattggtgg atactgtcaa aagccaaaca acgggagcaa acagttttga
     9121 tataaaatat aaaagtcccg acaaagacag cgctaatctt tcagcatttt tcattgcaat
     9181 tctttttgga acaagttcac ccattacaag tgttatataa gacagtaata atgttatcag
     9241 tactacagaa acggaattta agactgaata aggtatattt acacctatat ttattaatag
     9301 tccggtgagc ctgcctgcaa agttttctgc agcaaaagca ccccctaata gtgctgctaa
     9361 tgtgatggca acttggataa tagaaagaaa ttgagacggg ttatcggtaa gttttgaaag
     9421 cctaatagca cgcttatcgc cttccagcat catttttgct attctgttgt cattcattga
     9481 tattaccgca atctcagcag atgcaaagat tgcattgatg aataataaaa tgaattgtaa
     9541 aaccaatgga ccccataacg agccttcttc taacaaaatt aattcctcct aattaagtta
     9601 aataattaca agaataataa catagatatt ttgctccgtc aatattcatt tattaatgta
     9661 aactttataa aatcaatgta tactgaggat aactaaataa atattattaa ttttttatac
     9721 aaaaactagc attttttgtc aaaaataagt tgctataatt taatctatat tgtagaatta
     9781 gtataactct atttaatgga ggcaaatatg ttttttgaag ctttaatatt aggaatagtt
     9841 atcggctata taagaagggg aaagctaagc agattatcat atgttaattt cagttttaga
     9901 ttcttgattt acatttcagc tttgttttac ttgggcatta tagttataaa tttaggcctt
     9961 tatgattatg aatcatttct ttattcaaca tttcttatcg gctcaatagt tttaacagga
    10021 ttatttttaa tagcaaatat aagcataaaa tatatgttta ttccattaat aggacttgca
    10081 ttgaatttgt tatcgttttt aacaaacaat tttaaatttc cgctgtctcc ggatgctgca
    10141 gcaaaaatat acggtgcaga agcagccgag cttttaaatg ccggcaaatt attgttctat
    10201 acatcttcag agacagccac actcagcttt ttaggcaata taattcctat aggaaactgg
    10261 ttcgtggtaa gtataggcga tattatagcc tccttgggtg ttgtattgat tgttcaatgt
    10321 attatggcag ataaatttat acaaaccaga agcaaaatta ctttttcaaa agatatattc
    10381 agataaataa ttaaaaaagc gcccttaggc gcatttttta tttttttaca aatctatatt
    10441 ctcaaattta ttgaactggt caattccgat attgccgctt gaaattaaaa agacaacttt
    10501 atcttccggt tcaaattcaa cagccttttg caggactgca cctattgtaa tagaggaaga
    10561 aatctcagcc aataatttcc ctatcgttaa taagaattta gttgccttaa gaatattttc
    10621 ttcatgaacc gtaacaattt tatcaacata tttttctatt atggggtaat tacgttctcc
    10681 tggtcttaag gtttgtaagg catcagccac tgattttgag tttgggtcca aagaaacggg
    10741 atgacctgct ttaaagcttt cactgtaacg aggaaccaat gccggttcaa ctccgataac
    10801 tttaactttt ggattaattt ctttgactgc tgcagaaagc ccgccgatta aaccgccgcc
    10861 gccgatggga acaacaatat aatctgcatc cggaagctgt tcaagtattt ctaatccggc
    10921 agtaccttgt ccggcaataa tttcataatc atcaaacgga ggtattaagc tccagccatt
    10981 ttcttcactt agttgtcttg ccacattata tcgctccgat ggtatactgt agattatttc
    11041 agctccatat gattgtattc cttcaacttt tatcttggga gcattatccg gaattactac
    11101 tctggctttt cttcctaaca aagtagctgc atatgctaca gctttaccgt gatttcccga
    11161 cgaaacagta ataattcctt tattgagttc ctcctccgat aaagtcatta atttattaac
    11221 cgcaccgcga actttaaaag aattggtctt ttgaaatgat tccattttag tgtatacttg
    11281 acagcctaag tattcatcca agttatttaa acgaaggaga ggagtcttat acacaaatgg
    11341 tgcaatgcgt ttttgtgctt ctttgatttt ttcaattgta agcatgagct taacccccta
    11401 gactaatttt aataccatta attaagattc ttttattgtt tcggacaatg cttttgaaag
    11461 tcctgcaagt ccctgaattt ccgaaggtat tattatcttt gttgcctgac cgtttgcagc
    11521 tttttcaaac gattcaaaac tcttgaatgt aagcacttcc ttgttaagct caacatcctt
    11581 aatcattttt aaaccctcgg cggttgcctt ttgaacgctt aaaattgctt ctgcttcacc
    11641 ttctgcctct ctgattgttg cttccttctt agcttctgcc cttaaaattg ctgactgttt
    11701 ttctgcctct gcctgaagta tcatagattc cttgttgcct tgcgctacca atatagcaga
    11761 actcttttgt ccttcagcaa taagaattgc ctctcttttt tctctctcgg ccttcatttg
    11821 tttttccatt gcattttgaa tttcttttgg aggcaatata tttttaacct ctacacgatt
    11881 aatttttata ccccaagggt cggtagcatc gtccaagacg gcacgcattt tcgtatttat
    11941 tatttctctt gaagtcaatg tttcatcaag ctccaaatca ccaataatat ttcttaaggt
    12001 tgtcgcactt aaatagtcaa tagctgatat cggcctctcg accccgtatg tataaaactt
    12061 ggggtctgta atttggaaaa atattaccgt atctatctgc attgttacat tatcctttgt
    12121 aataaccggt tgaggcggaa agtcaattac ctgctcttta agagatattt tctttaccac
    12181 cctgtcaatc aagggaacct ttacatgtac tccaacaccc catgttgctt gataagctcc
    12241 aagtcgctct ataacatagg catgtgcctg aggtactatt ctgatatttg ttgcaaacaa
    12301 tataactact atcaataaca ataaaaccag acctatatat tccatatact cctccttatt
    12361 ttttctcaac aataagctta acgcctacta tttttttaac tattactatt tcgtcctttt
    12421 caattacctg gtcatcaaga gaccttgcag cccacatttg tcctttcacg tttactgttc
    12481 ccgtattatt aatattatca attgttgatt caaccttaaa gtattgccct attaatgatt
    12541 cagcattggt tttcactctt ccgaccttta atttctccat tgcaatcggc ctcgtaaata
    12601 taagacatac tatagaaacc aacaaaaaaa ccactatctg tacttctaat cctaaaccgg
    12661 tcaaataaat caaccacgcg aaaattgcgc cgatagcaaa ccatattgtt gttaagctaa
    12721 cagtcatagc ttctattgct acacaagcaa ccactattcc cagccaggca taccctatat
    12781 attccattga tacctcctat tacgggtaca ttccttaagt tgtgtccccc ttctaataac
    12841 tttacattaa ttatatacca caattcaaac ctggtaaaca gttttcaatc aattatcccg
    12901 cttaaggttt gatatggatt tacataagtg ttataaatac tcactgcata atgcaagtgg
    12961 ggcccggttg aaaatcctgt tgtacccaca gttccaataa catccccctt tttaacctca
    13021 tcgccttttt ttacattaat tgtatctaag tgatagtagg aagtaaaaag ccccattcca
    13081 tggtcaataa caaccgtgtt gcctggagaa agaagtcctt ccatagcgaa tgtaaccctc
    13141 ccgttatttg gagctacaac aggagttcct tttggagcgg ctaagtcaag acctgagtgc
    13201 cgcgaagaag aaatttcatt attcacatat cttatttcag caaaatccgt tgtcagttct
    13261 ccttcaacag gcattataaa tgtaccctcc cacaactttt cctttgccga ctcggttctt
    13321 gcaggtttta caagctgtgc aaattcatag attgcagcat tatcattatg tgtttcgttc
    13381 atttcttcgc taactgtaag atattgagtt ttaaaacttc tatttgacac agtcaatgtt
    13441 ttttccaatg tatattcatc ttctgcccct tcatttacaa ctgctataac attatgttca
    13501 ccgggctgtg tatacaggtc tattggcatc atataatagt ttttatcttt gtatgcataa
    13561 agatcaaagt tagttttaac tgcatctgtt tccaatttta ctgtttcatc atcatttaaa
    13621 ttctcaatac taatcaacaa gatgttgccc ggataattct ccttggttac tatattaatt
    13681 tgtgcaggta tgtctaccga gatagaaaat ttcaacttct gacttccgta tgactctctg
    13741 ttttccttaa agtgccactg gctttcacat tcaaccaggt attcaccgtc actttttata
    13801 ttacttatga gctcagacaa attttttcct gtgccgaaca aactgccttg ctcataaatt
    13861 cttattattt ggctatcagg ctgcttttca tgtttaatat ctattgagtc ggagtgtgac
    13921 actgttattt tttcattctc ttcatatttt cccgttaata gtcctgcatt gtttacaaaa
    13981 tctccttcta tatttctgct gttccaattg tattcaatgt caggatttat ttgttgcccg
    14041 tttacctcaa tatacgaatt atacattgct gaaatatata tgtatgaaaa agcatcgctt
    14101 aaaaacaatt gttttgcaat attgtctcta attttaaaca ctgttttatc ctgagcaaga
    14161 tatgcagttt gattgtctaa atcaaagtaa agcttgtaaa cttcttcatc attttctttt
    14221 tttagtataa tttcatagct caataaggat gttatatctt catctgtttt attcttctta
    14281 tttactgcat ctataattat atccgcatcc ttgctgtctt ttggtaaaac aatcttaaca
    14341 ccgttttgta aatttactgc ttcaatttcg gtaacatcgg aaaagaaagt gctgcaaccg
    14401 gaaaataaaa cacacaatat aacaataatt attattttct tcataatacc tccatcaagt
    14461 agtttttgat tttgacaaag tattatacat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn ttatctttct tctctgaaat aatttactcc gcagcttgcg ggctgttggt
    14641 tttcctttga tttcctaatt gaagttacta ccaataccaa ggctgttata gcaaatggaa
    14701 gcatatcata aaatgcattt ggaattgtaa aaattgtttt tggaaaataa tattttaaaa
    14761 tattgaatgc tccaaatata aatgagccga aaattgctct taagggtttc catgaagcaa
    14821 atataacgag agcaacggca atccatccct gaccgtttac cggattgttc atccatacac
    14881 cgccgttgat aatcatggag cagtatgctc ctcctatacc gcatataccg ccgccaatga
    14941 gtacattaat atactttagc tttgttacct tgattcctgc tgcatcagct gcaccgggat
    15001 tttccccgat agctctcaca ttgagccctg tctttgtgcg agagtagtat ataccgcatg
    15061 ttacagcaat aactacaccc aaatatacaa aaatattgta ttgaaataat aatggtccca
    15121 aaacaggaat agtgctgatg ccgggaatat ttatattgct tatttgtgca gttatttgct
    15181 caggaagctt taaagaattt gaagcagaat ttacaagcat aaattctccc gcaaagtttg
    15241 atatacctac accgaaaatt gaaagggtaa gaccggtaac attttgattt gccataaatg
    15301 taattgttaa aacagcataa attaaagcac ccagcatgcc tgccaaaaaa gctgccaaaa
    15361 gggcaatata aatactattg ctgaagaatc ctgccatgaa tccggcacat gctcccatag
    15421 ccatcattcc ttcaactcca agatttaaat gtcctgcctt ttcattcata atctcgccta
    15481 ccgtaccaaa taacagagga gttcccgcaa ataccgcagc aattaaaaat ttaatgaaca
    15541 tgttatttcc ctcctttttt ggtaagtaca aatttatatc ttatgaaaaa ttcgcatcct
    15601 aatataaaaa acagaattat accctgcagc acacttgcaa aatatgttga caatccatag
    15661 gtagactgca tgacgctgct acccttatca agtatgctga acaaaaatga aaccaaaagt
    15721 attccaaaag gatttaaata tgccagccag gatacaatta ttgcagtaaa tccaactcct
    15781 cctgctacag aagatgctaa ggtcatgtct gaaccggcag cctgaatcat tcctgtaata
    15841 ccgcaaattc cgccgcttat aatcatggtt cgaagtatta tttttttaac atccatacct
    15901 gcatatctgg cagtagattt actttcaccg actacgctta tttcatatcc gtgtttggta
    15961 tatttaagat atatgaatac tatgacaaca agaatcagag caataatcca tccgaagtgc
    16021 actccaagga ccttatcgat ttgagcatta gcagtaaatc ttgctatttt cggaaaacct
    16081 gacgagccgg ggtccttcca gggaccatcc ctcaggtaag taattatatg caatgctata
    16141 taattgagca tcaatgtaaa aagtgtttca tttgtatcat attttgattt aaagtatgta
    16201 ggtatcagtc cccacagacc tccgcctatt gctcccgcag caaacataac aatcaataac
    16261 aaaaaatgag gccaattatc gtgaaacagt gcaaaatatg aagcgcagac agcaccgact
    16321 attatctgtc cttcggcacc tatattccaa aatctcattt taaatgcaat agaaacacct
    16381 aaggatgaca ttaataatgg aatcataaat ttaactgttg cctggaatgc cattttactt
    16441 cgaaatgccc cggatataat agttccatac aatttaaggg gattaaagcc taagagaagt
    16501 atgaataatc ctcctgcagt aagggctaat acaaaggcag ctattcttat ttttaatata
    16561 tcattgcttg agcgctcgga tatttttacg gtccttataa aaggtgtatg acttctgtta
    16621 ttcatctgag tcaactcctt ccatctttct tcctgccatc ataagcccga cttcttcctt
    16681 tgtagtattt tttgcatcca caagacccat taattctcca tggcataaaa ccataatcct
    16741 gtcggacagc tccaacaaaa catccaaatc ttctcctata aataatattg caacattctt
    16801 tttcttttgc tcatttaata aatcataaat tatataagaa gaatttatat caagtcctct
    16861 gacgggatat gcagtaatca aaacattcgg ttcagattca atttctcttc ccaaaagaac
    16921 cttctgtaca tttcctcctg aaagcattct taccggagtt tcaacattcg gagttgtaat
    16981 actcagttta tcaaccaatt cttcggctaa ctgcctggac ggttttttgt caattataaa
    17041 acctttgttt ttcttgtatg atttaagcaa catattatca atcatgtcca tagagcctac
    17101 taaccccatg cctaacctgt cctccggtac aaaactcatg cttatgccta aatttataat
    17161 ttcatcggga gttttcccta taatattttc cttgttatat aatacggctc ctgtcttagg
    17221 atgcataagt cctgcaatag tttcgcacaa ttctctttgc ccgcttcctg caacacctgc
    17281 aacacctagt atttctccgc tttttagctt aaaattaata ttgtttaaac ctattgtgcc
    17341 gtcctcattt acaacagaca ggtctaccac ctttaaaact gtcttctttc tgcttgtttc
    17401 gggacgcttg atttcaagac tgataggtct tcccaccatt aattccgtaa gctgtttctc
    17461 attagtctcc cgagtattga ccgtatcgac tacctttcct tttcgaagta ttgcaaccct
    17521 gtcactgatt gctaaaactt catggagctt gtgtgtaata ataattatcg cattcccctg
    17581 ctctttcatt ctgcgaagta tcacaaagag cttttcagtt tcctgaggtg tgagtaccga
    17641 agtgggctcg tctaaaatta aaatcttcgc ccccctgtac agcactttca ttatttcaat
    17701 ggtctgcttc tcgctcacgg acatattgta gactttttta tcaggctcta tttctaaacc
    17761 gaatttgttg ctcatttcaa gaattttttc ttttaaagca ctgtttttga cgtatcttcc
    17821 ctttgtgcct aaaacaatat tatccattgc actgaatact tctaccaatt taaaatgctg
    17881 gtgaatcatt cctatgccta attttatagc ttcgtgtgga tttgttatga aaacttcatt
    17941 tccatcaata aatattgtac cttcatcagc ataataaatg ccggataaaa tattcatcaa
    18001 tgtggttttc cccgagccgt tttctccaag caaagcaaga atttcaccat atttaattcc
    18061 aaggttaaca tcttcgtttg caattacttt tccaaaggat ttagtaatac ccttaagttc
    18121 tattgcataa ttatctatcg ttttcaacat aatcaccttt tttgtcatat cctgagcgta
    18181 aacgaaggat ctcagggttt aaaaatgaga ttcttcgagc taaagctctc agaatgacac
    18241 ctagcttatt catagcaatg tattaagggg ttggcaacaa ccaaccccat tgaattattt
    18301 ttctactaca gttttaaaat accaattgat tccgccggta atagtaccgt catccaaggt
    18361 tttgccttct tctccaactg tggagccgtc attagtctct attactccgt caaaaacatt
    18421 gaaggtgcca tccataatag attgttttgc ttcctcgatt ttttcaacgg ttccttctgc
    18481 agcaatactt tcattaatag gtgaaatatc aatcatgcct tccttcattc caccataata
    18541 attttcactt aaccaagttc cgttaataac tttttctaca gcccatgtat aataagctcc
    18601 ccaattccat actgcagatg taatggtagc ctttggagca tccttagaca tatctgaatt
    18661 atagcctata ccaaaaactc ctgccttctc tgcttccaat tgagggttgg gagtatcgca
    18721 atgctgggct attacgtcac agccttcagc tatcaatgct tttgcggcat ttgtttcttc
    18781 ttcgggtgaa taccagctgt ttgtaacctt tacataaact tttgcttcag ggttaactga
    18841 atgcacaccc attgcaaatg catttagtcc gcctgttacc tctgaattat ccttacccat
    18901 tgctgccaca tatcccaact tattagtttt tgtttttaat ccggctgcaa ttcctgttaa
    18961 atatcttgcc tgatagattc ttccgaaata attgttaaag tttctatcat tgcttttata
    19021 ccctgaaccg tgtgaaaata ttacatcagg atattcttcg gctaaggctt ccattgtatc
    19081 catgtacccc cagcttgtac cgaaaatgat gtgtgctcct tcttcaatac attcgcgtat
    19141 agctgcatca gtagctgtag ggtctgtatc attaacattg tttttacgaa ttatctggct
    19201 gtcgctaaga ccaagtgctt tctgcatttc tattattccc tgatcgtgtg caaaggtata
    19261 accggagcca tcagcaggat ttccgatatg tattactcca accttaatat cttccttggc
    19321 aattgcagga aatattcctt ctccggaaac ttccggcccc tctgattcgg gttttgtttg
    19381 ttgtccgttg gaaccgcatg cagtaaacaa tgaaagtatc attgtcagcg ctaaaataat
    19441 acctaaaaat tttttcattt tatcctccta tgtaataaac tttgcatcaa gaaataaaaa
    19501 atgcgaaaag tatccctaat cgcatcaata gaataatact atcaatagtc aaacaattta
    19561 aggttgtttg gtagaaacta tggaaccata ttatccatat tatattgatg cagtctatat
    19621 attcaacata attataactt aagctgtgac aataatcaac aaattatttt gttatcagcc
    19681 cggtgggaca gggttcgcag aaataattta atattaatag ttgaatacta actaatgctc
    19741 agatacagaa tttatcagta attagaaggt cagtcttaaa tgtcacattg aagctttccg
    19801 cttattccaa agttttcaaa ttccatttct cttataacga ttcttacggc ttcctttgga
    19861 caattggcgg ttctcgatac tacttccgtt atttccttaa ccatttctcg tttttggtca
    19921 actgtccttc cttttagcat ttctacctga ataatgggca tgattccacc tcctctttct
    19981 caatatggta aatttaatac tttattattt ttttgtgtac ataatcggac aaggtggtca
    20041 tcagcattgc ttgatacaaa atattaaatt caattatatc tcctaattta tattctgtgt
    20101 cgcaatcgtg aatatccaaa atggtatggt cgctgctggc tcctaataca atcatattct
    20161 tatcctttgg aacaagcctt gatgagtccc ctaaatcctg atttcccaat gcaataattg
    20221 ccctttttct gattcccctg tcttcgtaat gaggcaaatc tccaaaagca tttaccatta
    20281 attgtccgat ggggtgagtc ggtttttcat ttatttcaac aatttgagcc tttaaaacaa
    20341 atacatcagg gtctagacct tcaatatttg tatcccagta aagaggcaaa tcttgtgtat
    20401 tatttattcc ttctccaata cgaagatgat ttattttctt tggaactccc cctctcacca
    20461 aaagaggcag cgtggttgtc cctcctccgg atattatgtt tagttttctg tttagttttt
    20521 cttccatttc tgttgctgct tctgctaatt gacttaagtt ttccgtagtt ggagcaactg
    20581 agccgtaaca gcttaaattt gagcctatac cttcaagata taaattatct gattcatatt
    20641 caacatattc ggctaatcga atcaattctt ctttataaaa tactccttct cttaaatccc
    20701 ccaaatcata cataagcaca acgccgtaga ttttattttg agcttttgct tctttattta
    20761 gcaaattcag agtttctttt tcagaaacca aggatatatc actgtattta acgacatcct
    20821 caatttcaca catcatgggg attctgacca ataacaaagg catatcaaat cccctgtcct
    20881 tcaaggcttt aagctgctca attcgggagc tgcctatttg ataacagcct ccttttacca
    20941 tttcttcggc accttccgca attccgccga atcctttaat tatgcctgca accttgattc
    21001 cgttatcttc acatagtttt gtaacaattt tggtattgtt gtaaagtttt tttaaattga
    21061 attcaagcat agggtatctt tccataggag cctcctatat tttttttcta agctttataa
    21121 aaaagtcttg taaaataccg gtgcattctt tctccaatac accaaactta acctccggct
    21181 ggtgatttag ctttttattg tttgttatat ccaaaacaga gccgcatgca cccgttttta
    21241 aatccctggc tcctattacc agcctttcga ttcttgaatt gacgattgcg ccggcacaca
    21301 tggggcaagg ctccaaggtt acatacatgg tacatccggg aagacgccat ccgcccaagt
    21361 tcttacaggc atcctttatg gctgttattt cagagtggag agtggcatcc tttaatgttt
    21421 ctttttgatt gaaacctctg ccgacaattt ctccctttct tactattacg gcaccaatgg
    21481 gaacctcatt tatttcatat gccttgtatg cttctttcaa ggcttcctcc ataaatactt
    21541 catccatagc tcttccttat tacattttat ttaattatag ttttttcatt aattcaagtc
    21601 aacaatattg tctaagagcc tgtcttaaca taagcagaaa ttgcatgtta tttaatatac
    21661 cttttcaaga aggcttgtct ccttatatta cctgtttact ttcttcacaa taaaatatag
    21721 aattaattat atagttttaa acagcgaaag attccctatt agaggtatgg ggacacacct
    21781 tcaatgaagg gatagccttt ctgggtgagc attgaaggaa tgtccccgta aatatccata
    21841 cttggaacaa atgttaattt gattaagtct aatatatgag gaggaggtac tatgaaaaca
    21901 ataaaaaaat tatgggctct tggtctttgt ggcatattgg taatcaatat gaattcaatg
    21961 gcttacgctg atgttcttga tgatattata ggcagcagta ctattgaaga tagagaagat
    22021 gctgaaaaca ctgaagatgt atacacagat gttactagtg aagacgtaac tggaaatgat
    22081 atcacagtca ctgatgaaaa tatagcaaaa tattcggttt tgttttccga cattgataat
    22141 cattgggcaa aagaatggat taaaaatgca gttggtttag gctttgtatc aggatatgaa
    22201 gatggaacct tcaaaccgga cagaactatt acaagagctg aattttcaac aatgttaaac
    22261 aaggcaatga aaattgaaat aaccgaaaat ataaactttt ccgatgtaag agaaaaagaa
    22321 tggttctata aggaaattca aaaatctgta gctgccggat ttttttcagg atatgaaaat
    22381 aatacattca gaccaaacaa tcccatcaaa cgggaagaag tagcaaaagt tgttgcaggt
    22441 gccataacaa cgggaaacat tgacggtgaa ggtgctacgc ttttaaagga ttacagtact
    22501 attcaggaat gggcaaaaga tagtgtaaat actgtttaca acaaaggata catactaggc
    22561 tatcccgaca aaacgtacat gccttctaaa gcattaaccc gtgccgaagc agtgaaaatt
    22621 atctttgaaa tagttgataa tgaaaatatt gaatacgggt ttaatattac aaattacaat
    22681 gaaacttaca gcagcgcagt agtggttgga aatttaaatg ttttggattc tgtcggcaac
    22741 ggtaacgtat atattaaaaa tgttgtggtt ttaggtgaca ttgtaatttc ggctaaaaat
    22801 gttaaatcgg ttgagcttac agatgttaag gcaagaagaa taatagttga aggtgaaaat
    22861 aatcctgtaa gagtagttta caatgacaat gtaataatcg caaaaaaata gatataaaaa
    22921 gattcttcat cacgaagaat cttttttgtt gccttttatt tcagcatttc aagcgtattt
    22981 acaaaattct tatttaaatt aatttcattt atataatttt tatcaatatc agaaattttt
    23041 tcatatattt ctaatatata ttcttgttct cccgccataa ccgccttatt gaaacgagaa
    23101 tcgtaattca ttgacaactc aataaaggcc ttatcatttt caataaataa attttctata
    23161 tcttttgtca atgcacctct taaatcgttt aaataaacac tctttataac attattaaga
    23221 ttactaatat ttttaaagtc tgcatacaga tttcttccgc tgtaatagtt ctcatcgtaa
    23281 atataataga atttttgctt tttataatca attgaattca gcaatttgtt tgctaactca
    23341 tttttagttt catgctcttt tgtaagttta tctgttgcag ttaaagtata gtctcttatt
    23401 attttactcc catccttcat ccagtaagct attacaaaat tatttattcc gtaataatca
    23461 gattcatgat aaaatgtttg gtcttttaat acttcctcgt gaagttcagt aataatttca
    23521 attatatcct catcttcata agtaatgata ttcctgtttc ttttccattc cggaaaatct
    23581 gatttttcac ttgtatagtc gccccttaaa aacctgtcaa tttcatttat tgaccatctt
    23641 tgattgccca catatgccat ttgaacatct tccggctcag gaacaagatt ttcatattga
    23701 cttgcaaccc ctacggtgcc tcccgttatt acagcaaatg caaccatgct cactataaac
    23761 accttaactg aagatttgga tattctgaat gatttttcaa tgaatgcaat tattatatag
    23821 taggatagta ctgccaataa tgctgatata attaacctgt ttccaatact catatatctt
    23881 gaaaagccta ttgaaaatga tgctggtatt atcaaacatg caagtactgc cacaaaatat
    23941 ttaaatccat tgaatacaat aaacccggta ttttcgtttt ttctcctttt tataaattta
    24001 tttataagca gtgatataaa tattaatata gttggcaaca aaagcaaata taccaaatct
    24061 atcgatttac tttccacata cctgacgaag tacaaataat caatcctgta tatattatta
    24121 acgaaataat cgcttaaaac atccgcgctg aaccctaata ccaggttttc caatattgaa
    24181 aatataaaca ttataaccaa cagtattatc aggggaaggc aaaagttaaa tattgtcatt
    24241 gctcctgcca tcaaggtatt tcctgacaat attgacgaca aagacgctaa cagtattatt
    24301 gcagaaagcc agggaataag cattttaaac actttaagta cttcatagtc cagcccctga
    24361 aaaattaccg acaagacaat aagagccagt aaataaactg taaaaactgc atttgtcact
    24421 atgttaataa ttatctgtct gcctaaggtc acaggctttg agctataata tgttaacttt
    24481 gattcattgt ggaaaaaact gttatcaacc aaacagttta tcaaaaccaa aacaaaaagc
    24541 tcaactatac caaacattat aaaaaattct tcatctactc tcaaaatata tggtattaaa
    24601 agtgttgtta aaaaaattat agccgttgac agaagtaccc aatttttctt ttccttaatt
    24661 gaatatatta atagcggcat aaagttattt ttcttttcca taacccacac ctcctaattc
    24721 tgtaataaat atttcttcaa gactcattga cagcttatcg aacaacaaaa gagtatattt
    24781 gctttccaaa tattttttaa gctcatctat gtttcctgtt aaagttgcaa taaatacgct
    24841 gccgattttc acaacctgaa gcacattgaa ttcttttagc ttatcagggt caaattcacc
    24901 ctccactgca aattgaactc ttttaatatc ctctttcatc ttttcaaggt tttcttctct
    24961 taatattctg ccttcatgca ttattcctac tctgtcgcat atattatcca attccttcag
    25021 gtcgtgggaa gaaataacga cagtcatgtt attatcaatt atttctttta taagaacatc
    25081 ccaaaacttt cttttaatta ctgcatctaa tccatctaca atttcatcaa gcaagagcag
    25141 tctgggctga gccgctattg acaaaacaaa tgctgcctgc tttttttgac cttttgacat
    25201 tttgtttaat actttatttt tatctatgtc aaagtatttt aacaaatatt caaatttttc
    25261 ttcagacata ttatacatca gcttttcata atggaataat ttgttcaatg tatactgata
    25321 gggaaagtac agcgtgtcct gtacaaaata gaattcttta ataatgtccg agccttccac
    25381 tacctcttcc ccgttaaaag atatatggcc ttcatcttgt ttgtagactt tcattatatg
    25441 ttttaatata gttgtttttc cgcaaccgtt agagccaact aaaccataaa tccggccttt
    25501 ctcggcattt atgtttatat tctttaaaac ctggaaatct ccgaattttt tgcttaaatt
    25561 attaatcgtt aacatcatgg cctccttttg tgcctggagt taaaatatct ctgaccattt
    25621 caattattct ttcattcctt aacccaagtt cctttaagga tttcactacc ttatataatt
    25681 catccttcat attctttaaa tgtagattcc ttaattcatc agcatctttt acaaagtttc
    25741 ccacgccctt aatactatat atataattgt cttcttccaa atatttataa gctttttgga
    25801 tagtattggg attgattcta atcattgatg ccaattctct tactgaaggc aatttttcat
    25861 caggttttaa ggatttagaa aatatcagcc ttactatttc atcataaatt tgttcgtaaa
    25921 tagcttttgg attatcatta tcaattcgaa tcaatactac acccccaatc atgcattgac
    25981 gcaagcaacc tttttactaa catattcaaa caatcgaccc tcatattccg gtataaggtt
    26041 aaaattatta tagtcatccg gaataataag tgatatttcc ttattaaagg gtattattcc
    26101 ttttaacaaa taagatacaa aacttgagca gacaaaatga tattctcttt gatagggtat
    26161 gttaaagcga attaaaaaaa catttaaaaa actatacctg tacttatggg gtttagcaag
    26221 aaaatcatta agccgcttgg ttataatatc ataatcttca tcagttactt caaacttata
    26281 tattttacat ttagtttcag gatgcatttt aaacacgccg ctgtttatgt
//
LOCUS       DAOSHP010000015        24612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1602_length_24612_cov_10.014375, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000015 DAOSHP010000000
VERSION     DAOSHP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 24612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24612
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_24612_cov_10.014375"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLB33_02835"
     CDS             complement(<1..521)
                     /locus_tag="PLB33_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOG62489.1"
                     /translation="MKKIKKTLLLCLIIIMLTNTLVFADININENIKGALLGDLEGEI
                     LYDYNINQQLAIASISKLMTYTVMMDKVSAGEISLDDDVTISGHAAATEGSNFGILAG
                     ETIKLRTLVEGMLIVSGNDCATAVAEYVGKTQDNFVSMMNAKASELGLLSASFINPHG
                     LPINDEETGQNYMS"
     gene            complement(619..1548)
                     /locus_tag="PLB33_02840"
     CDS             complement(619..1548)
                     /locus_tag="PLB33_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG62490.1"
                     /translation="MVIKKDFYYDSADKKSKIHAVKWVPDAEIRGVLQIAHGMLEHID
                     RYDCFARYMAENGILTAGNDHLGHGDSVLVEEDRGFFGEGDGNKLVTKDMRKLREILK
                     EEYEGLPYFILGHSMGSFLVRQYISIYNDLNGALLVGTAYQPYLSVKAGLIISKLMAV
                     FKGWRYRSKFINKLTIGGNNKYFEPAKTKSDWLSRDEEAVDAYVKDRKIDFIFTLNAF
                     YYMFKGMLYLYGKEYMNKIPKTLPVILLSGEQDPVGNFGKDVKRLYDSFKKELTDVTI
                     KLYKDDRHEILNELDKENIYEDILKWIYEKSSK"
     gene            complement(1575..2126)
                     /locus_tag="PLB33_02845"
     CDS             complement(1575..2126)
                     /locus_tag="PLB33_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOG62491.1"
                     /translation="MIKEFEGIKPIIDEKTYIADGAQIIGKVTIKEYSSIWFNTVVRG
                     DVNYIEIGRYTNIQDNSVVHVADDYPAIIGDYVTIGHNATVHACKIEDHCLIGMGAII
                     MDGAVIGRGSIIAAGAVVTNNQKIPPFSVAAGIPAKVIKTVPEEDIIKIHSQALKYKH
                     VWSIRYGIMPDCGGESYDGGEII"
     gene            complement(2196..2609)
                     /gene="ndk"
                     /locus_tag="PLB33_02850"
     CDS             complement(2196..2609)
                     /gene="ndk"
                     /locus_tag="PLB33_02850"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOG62492.1"
                     /translation="MSDFLGKERTLVLIKPDAIERKLMGEIISVYEKNFNIIDMKMIK
                     PSVEIAEEHYYEHRDKPFFNELISYIIRGNVCALVIEGKNVIDRVRKINGATDPKNAE
                     PDSIRGKFALSKQENSVHGSDSKESAEREIKIWFG"
     gene            complement(2607..3002)
                     /locus_tag="PLB33_02855"
                     /pseudo
     CDS             complement(2607..3002)
                     /locus_tag="PLB33_02855"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
     gene            complement(3306..4103)
                     /locus_tag="PLB33_02860"
     CDS             complement(3306..4103)
                     /locus_tag="PLB33_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HOG62493.1"
                     /translation="MANKYHDFSPKEVRQLIREEKIQEPTSGMCGGYAQANLVILPKK
                     YADDFKLFAEYNPKPCPILEITEPGSYTTTKTADKANIITDIPKYFIYKNGVRVDEVL
                     NAEKYWGDDFVCFLIGCSFSFEEAMIREGIEIRHISMGRNVPMYKTNIEAKPAGIFKG
                     PYVVSMRPMKPEDVDKAYEITGKFPHVHGAPIHHGDPAEIGIKDINKPDYGEAVIIKE
                     GEVPVFWACGVTPQAAIENAKPEIVITHAPGHMFITDILNEDIESIF"
     gene            complement(4166..4585)
                     /locus_tag="PLB33_02865"
     CDS             complement(4166..4585)
                     /locus_tag="PLB33_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62494.1"
                     /translation="MEVDIINHKITEGLSITYQKQTNASDAYVSNTSSIENLVSTPAL
                     LTMIVEASCKMLDSLIPQQCLTVGNRLEISHINPTLVGDTITLILTVEKVDGNKILLD
                     IVGADSNGRICLGKYHKVIVERNKLMENAYKRSKDWQ"
     gene            complement(4616..5683)
                     /locus_tag="PLB33_02870"
     CDS             complement(4616..5683)
                     /locus_tag="PLB33_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOG62495.1"
                     /translation="MSSVKVKKGGFLTTVQDLGRIGYQKFGMPVAGVMDNFSYRVANF
                     LVGNNEGEAVLEATLLGPELEFGCDMSFAVTGANMKPMLNGKEISMWESYKVTKGDVL
                     SLGVVTSGLRAYIAFSGSIEVPLLNNSKSTYLKTGIGGFKGRKLMNDDILEIKVSDKI
                     VYGKYMDPKYIPEFKRKGNVRVVLGPQDDYFTEEGINTFLSPEGYKITKEADRMGYRL
                     DGKTIKHIDKADIISDGALFGSVQVPANGLPIILMADRQTAGGYTKIATVISTDLPML
                     AQMGEGCQLVFEKITLEEAHKLYKEYEEKLSEIEKQIANSQRVIEAPVEKAAYVNIGP
                     VRRMNIVINGVNYVVDVQEIQ"
     gene            complement(5676..6422)
                     /gene="pxpB"
                     /locus_tag="PLB33_02875"
     CDS             complement(5676..6422)
                     /gene="pxpB"
                     /locus_tag="PLB33_02875"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOG62496.1"
                     /translation="MYEKIKYLSAGDKAVVMEFGNEISKEINAKIRNVVKLIDEAKID
                     GIVELLPTYRSLMIMYDPLKIEYSDLISTLDSMSSKQVESSDEKIRIVEFPTVYGGEY
                     GPDINFVAEHNNITVDEVIKIHTGTDYLVYMMGFTPGFTYLGGMSNKIATPRLSSPRT
                     KIPAGSVGIAGAQTGMYPSETPGGWQLIGRTPLKLYDPDKEPPVMLSAGDYVRYVSVS
                     EEEYLEIKKQVEEGTYEVKVIISEGGDLRE"
     gene            complement(6450..7646)
                     /locus_tag="PLB33_02880"
     CDS             complement(6450..7646)
                     /locus_tag="PLB33_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOG62497.1"
                     /translation="MSERDKSQDKRILIGAAFLMATSAIGPGFLTQTTTFTAQLGPTF
                     GFVILVSVLFSLIAQVNIWRVICISKARGQDIANKVFPGLGYVVAILVAMGGLAFNIG
                     NVGGAGLGLQVLFGLDLNVAIAISGIIGIIIFSVKQVGNAMDKFATFCGALMIILILY
                     VSISSKPPVGQAIKETFVPSSFPYLQILTLIGGTVGGYISFSGGHRLIDAGIVGEENF
                     DRVNQSAFMGMGVATIVRILLFLAVLGVVSTGVTLDASNPAADAFKIAAGTLGYKIFG
                     MVMAAAALTSVVGAAFTSVSFLRTLIKPIGDHVNITTIAFIVASTAIQIFVGSPAKVL
                     VIVGSLNGLILPLTLGTILVASKRKDIVGEYKHAQWLFILGWIVVAVTAVLGLRSLTG
                     IAALWK"
     gene            complement(7744..8511)
                     /locus_tag="PLB33_02885"
     CDS             complement(7744..8511)
                     /locus_tag="PLB33_02885"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOG62498.1"
                     /translation="MYKVDLNSDVGESFGNYKLGLDSEVLKYVSSANIACGWHAGDAM
                     VMQKTVDFAIKEDTCIGAHPGFPDLMGFGRREMKVTKEEAKNYVKYQIGALWAFAKSC
                     GTQLQHVKAHGGLYNMAAKDPELAMAIAEAIYEVDKDLILMGLANSEMIKAGKAVGLK
                     TANEVFADRAYNTDGTLVSRKLEGSMITDETLALTRVVRMVKEGKVQAITGEDIDIKA
                     DSICVHGDNPKAVEFVKTIRKALEDEGIQIMPLKDFI"
     gene            complement(8686..10095)
                     /locus_tag="PLB33_02890"
     CDS             complement(8686..10095)
                     /locus_tag="PLB33_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOG62499.1"
                     /translation="MKIRILIVDDEETIRLSLEEALKDLGYSISTAATGHIALEKVKE
                     YKPQIVFLDMRLAGENGLDILPKIKKIDKKVEVIIMTAYGDINTAVKAIKLGAFDYIN
                     KPFDLIDVEILIKRITENLKLQNKIYLLEKEKEKELNKKIIIGNHPLMNEVMNRIDIL
                     SKNDNVTVLIRGETGTGKDLVASYIHNHSIRRGSAMLKLNCGAIPQQLIESELFGFEK
                     NAFTGANNRKKGLLEIADGSTVFLDEIAEMPMEVQPKLLRFLDERKFKRVGGLEDIEV
                     DIRIIAATNKNLEKAIEDKEFREDLYYRLNVVPVYLPLLRERGDDILVLANNFLYEYN
                     CKFNKKIKGFTDRAKEALLLYSWKGNVRELKNVIERIVILVENEYIDIGDLPLEIRSA
                     LGGGNEFTKPSQDEMSSKLLSGGFSLENEVAEMEAEYIKLALKYCSNNHTKAAEMLGI
                     SRFAFKRRIEKYFYADNNA"
     gene            complement(10085..11842)
                     /locus_tag="PLB33_02895"
     CDS             complement(10085..11842)
                     /locus_tag="PLB33_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62500.1"
                     /translation="MRNSIEKKILIPFIIILMVSILIIVIVFVVNGYNLLLDNHINIS
                     YRNLEEMILTIEKVNDDIKDEIGAKDFIINFYKDLGKNNLIIFSDDDDEILLNTVLNN
                     NFIEEFMNEELNKGPESISVGNYIFTYQKYDKWNWVIAYGLNRDNLLIEVMESQQYII
                     LVALIALVFSMHASIFITHNISKPIKMLAEHCNKIVADGIYKDRIEIDRNDEIGTLAD
                     AFNNMLDTVQNNTDRLIEATKFNEDILRNVSAGIVTADQHGSVISINQTAENLLIPAA
                     EKIGLVKECLHEQIMETLNTKMKINKILAFDDEHGNKKYIDVTTSLLIGGDGSISGAI
                     CNFNDITERKRIENSMSTLDRLTTIGQFASGIAHEVRNPLAGMKTSIQVLKNRLCKED
                     NDMIASNKKLLNGVLFEIDRLNNLITDLLNFAKPSFPNIEKTDLMGVLDKSLNLIGET
                     AKKNNIEINIDNNCKNTVAYVDKAQTEQIFINIINNAFFAMDKGGTLNISFNNCSQKE
                     KFIEVIFHDNGCGIQSQDLAKVFNPFFTTNPQGTGLGLSVVYELVMGNRGEIDIQSAV
                     NEGTKVIIRFPINGGSNEN"
     gene            complement(12088..13362)
                     /gene="serS"
                     /locus_tag="PLB33_02900"
     CDS             complement(12088..13362)
                     /gene="serS"
                     /locus_tag="PLB33_02900"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004831860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOG62501.1"
                     /translation="MLDIKRIRNNPNEVEELLKRRNSELSLDKVLALDKARREKLVEV
                     ENMKAEQNKVSKQVPLLKKEGKDVTEILQKMKELAEKVKVLDGQVKEIDEKIEEELLN
                     IPNTPNPDITVGASDADNKEIRRWGEPRKFDFELKAHWDLGVDLDILDFERATKITGT
                     RFSMFKGEGARLERAIIAYMLDKHTLDHGFTEISPPFMVNRDSMIGTGQLPKFEEDMF
                     KLNQREYYLIPTAEVPVTNIYRNEVLDEKDLPMYMTAYTPCFRAEAGSAGRDTRGLIR
                     NHQFDKVEMVMYAKPEDSYNQLEILTNFAEEILKGLGLPYRVVELCTGDIGFSSAKTY
                     DLEVWMPSYNRYVEISSCSNFEDFQARRANIKFRPAEKGKLEYIHTLNGSGLAVGRTF
                     AAILENYQNEDGTVTIPNVLRDYMRGLEKITK"
     misc_binding    complement(13395..13654)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(13693..14247)
                     /locus_tag="PLB33_02905"
     CDS             complement(13693..14247)
                     /locus_tag="PLB33_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOG62502.1"
                     /translation="MIGKKIRNIRKNKNMTIVELSEKINVTSGYISQIERDLISPSLA
                     VLKRLSDALDVPLSVLFSEESSNDVITIPKEERTKVKLNNINVELEFITPILKNSHKQ
                     SEFEAFLFKMKPKTWISNHSITHESKEYIFVTQGEIECHVGEKIYTISKGDSIIVPEN
                     NNHMYYNKNDDVSEALCIISPSIH"
     gene            14510..16744
                     /locus_tag="PLB33_02910"
     CDS             14510..16744
                     /locus_tag="PLB33_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG62503.1"
                     /translation="MADNRVYLEEIKYLDEIINLLKNKLEYEKNQLENKKSDLIESRR
                     EMWENTTHSSADFDKLTDLNQYLSALQAQTFTYAELAKRILKHEKMLDSPYFARIDFT
                     EEGYDDIEKIYIGLFNLMDDETHEIKIYDWRAPISGLYYRNEVGPVEYKAPAGLIKGY
                     VSLKRQFKITKGKMEYFIDSNINILDEMLMVALSKNMTSKMKTIVETIQKQQDLIIRD
                     LYNDLLVVQGVAGSGKSSVALHRIAYLMYQGLNLKLRANNVIVISPNPLFSKYISNVL
                     PELGEESINEYTFENIFHKLFGNKLSMKSKSENFENIITAETEEQRNFLRSYDDFKGS
                     FVFSKIIDRFIEYYEHKLINFEDVYYNGKIIEKRELAKAFLLSGKLDMPTAKKLKIIE
                     SRILEKVRENRIERRNKIEKAVNKLNNHEFEIMSFTRLLSAKETLALTERLKKFTEFD
                     VFELYKKLFADIILFKKISKGLELPENIDEIISYTNTTLSDSYNIPYADGIALTYMKI
                     KAEGCDLFSDIKQVVVDEAQDYYPIHYLILKNLYKYARFTIVGDINQSIEKSNDLSLY
                     DDIISIFNFEKSSKVFLNKSYRSSYEIGKFSAKLSGDENYAEFFKRNEEEPVIFKFKD
                     INNLRDEIIKTIEKYKTEGFSSIAIICKDRKEASDLYFKLANKIKIKLIDYLDYDSVL
                     EGTLILPVYLAKGLEFDAVIVYEANDKHYNNEFDKKLLYVACTRALHRLSLFYTGNLT
                     KFLD"
     gene            complement(16796..16866)
                     /locus_tag="PLB33_02915"
     tRNA            complement(16796..16866)
                     /locus_tag="PLB33_02915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16832..16834),aa:Gly,seq:ccc)
     gene            complement(16873..16945)
                     /locus_tag="PLB33_02920"
     tRNA            complement(16873..16945)
                     /locus_tag="PLB33_02920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16910..16912),aa:Ala,seq:ggc)
     gene            complement(17036..17108)
                     /locus_tag="PLB33_02925"
     tRNA            complement(17036..17108)
                     /locus_tag="PLB33_02925"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17073..17075),aa:Thr,seq:cgt)
     gene            17247..17513
                     /locus_tag="PLB33_02930"
     CDS             17247..17513
                     /locus_tag="PLB33_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62504.1"
                     /translation="MDDYNQYYDTMNDYMYYDDINKDVYSSQYDMYDRRFDNRFDNKF
                     DRRFDRRYDRFFYDPMRYYRFPFCDRYGRCENPIWWLFWPFFFQ"
     gene            complement(17545..18105)
                     /gene="pyrE"
                     /locus_tag="PLB33_02935"
     CDS             complement(17545..18105)
                     /gene="pyrE"
                     /locus_tag="PLB33_02935"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOG62505.1"
                     /translation="MIDVIEILKECGALLEGHFLLSSGKHSNKYCQCAKLLQYPDKAE
                     KVISLVYEKVKSLNLDVIVGPAMGGITAAYELGRQLGIKTIFTERENNVMTLRRGFEI
                     QPGDRVLIMEDVVTTGKSSMETAKVLEEAGGIVVGIGCIVDRKVCEIQFPVYSSIELF
                     FETYDDKNCPLCEMGSKPIKPGSRKF"
     gene            complement(18125..19027)
                     /locus_tag="PLB33_02940"
     CDS             complement(18125..19027)
                     /locus_tag="PLB33_02940"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOG62506.1"
                     /translation="MANLKTEAMDLKFKNPIIAASGTFGFGEEFSQIYDLSILGGICS
                     KGLTLTPRSGNKGIRLWETPMGLMNSIGLENPGVDKFITEELPRMKTYNTIIFANLGG
                     HCEEDYLEGIYKLNDTNVDAVELNISCPNVKSGGIAFGVKSSVAYDIVSRVRKICKKP
                     LMVKLSPNAENIPDMAVACESAGADAISLINTIQAMAIDIKNRKAVFENVYAGLSGPC
                     VKPIALRMVHEAAKMVNIPICGLGGIMNAIDVIEFIMAGATLVQIGTANFIKPDIAVD
                     ILNDLEEFMDNENIKSLEEIRGII"
     gene            complement(19037..19765)
                     /locus_tag="PLB33_02945"
     CDS             complement(19037..19765)
                     /locus_tag="PLB33_02945"
                     /inference="COORDINATES: protein motif:HMM:NF000798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOG62507.1"
                     /translation="MKVLVTAVAENGQISKNIYRLSVKFDSEIKPGQFFMLKTTDNAF
                     LLPRPISVNDVNENLVSFLYRLEGQGTKKISSLRKNDEIQIFGPLGKGFEPDKLLGRV
                     AVIGGGIGVAPLLYLCKVLGKKADVYLGYRDKENMYIIDEFKSSARRCIVVTEDGSFG
                     EKGYVTDYISYDDYNVVVTCGPEIMMSKIATICRDKKIKCYVSLERRMACGMGACLGC
                     TVETKTGNKRACKEGPVFDSEELI"
     gene            complement(19788..20213)
                     /locus_tag="PLB33_02950"
     CDS             complement(19788..20213)
                     /locus_tag="PLB33_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOG62508.1"
                     /translation="MLNIDSVEKGLVIDHIKAGKAMEIYHYLNLDKMDCSVAIIKNAK
                     SKKYGKKDIIKVENNLDMDLKVLGFIDPNITINVIDNSVIIDKINLELPQEVENVVKC
                     KNPRCITSIEQEIVHKFRLADREKGIYRCVYCDTAYDKK"
     gene            complement(20213..21136)
                     /gene="pyrB"
                     /locus_tag="PLB33_02955"
     CDS             complement(20213..21136)
                     /gene="pyrB"
                     /locus_tag="PLB33_02955"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOG62509.1"
                     /translation="MLKGRSLIEPMDLSLAELDDILNLADRIMHDPAAYADKCKGKVL
                     ATLFYEPSTRTRLSFESAMLRLGGSVMGFSSAESSSAAKGESVADTIRTVACYADIAA
                     MRHPKEGAPMVAAMKANIPVINAGDGGHQHPTQTLTDLLTIQTLKGKINNFTIGLCGD
                     LKFGRTVHSLIKALSRYENIKFILISPDELRVPDYIREEILMKNGIEFMEVERLENVI
                     GELDVLYMTRVQKERFFNEADYIRLKDSYIVDNKKMSNAKKDMILMHPLPRVNEISVE
                     VDNDPRAAYFIQAKCGMYVRMALILKLLGVE"
     assembly_gap    21177..21276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21415..21627)
                     /locus_tag="PLB33_02960"
     CDS             complement(21415..21627)
                     /locus_tag="PLB33_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOG62510.1"
                     /translation="MPMKLNVGDVVKLKKKHPCGGYDWEILRIGADFRIKCTTCKRQV
                     WLPRSEVERRITKIIYQVENETSDRQ"
     gene            complement(21900..22544)
                     /locus_tag="PLB33_02965"
     CDS             complement(21900..22544)
                     /locus_tag="PLB33_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOG62511.1"
                     /translation="MIIDVIILIFTGYLFFQGYRKGFILTVFDTLGMIISFFLSKRFY
                     YIGEDFLLNNTKLFVKLHDYFEVKLSPSAFNNLSKIPVELQKFVNNIMASETSDTFAI
                     FVDNLSLLIIRSISFIVTFLSIYAILLILSFIVNTVMKLPLLNLANRLFGALMGIVKS
                     VMLLYLIFALATPLISFMHDKPLAQSILNSKSSKIFYENNIILNYLSYKGFYEN"
     gene            complement(22564..>22941)
                     /locus_tag="PLB33_02970"
     CDS             complement(22564..>22941)
                     /locus_tag="PLB33_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62512.1"
                     /translation="GTENIYFYNNNGKLMWKNSIYNKILDYEILQDRINILFEKGEST
                     ELISYNFEGKVIEIQAAPFGVHKLKIVDNKTLVYNNNSIYLLHSNKSDKIFEEFESFE
                     HFLIEGNTIYILFKNKIMSGQIK"
     assembly_gap    22942..23041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23042..23245)
                     /locus_tag="PLB33_02975"
     CDS             complement(<23042..23245)
                     /locus_tag="PLB33_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62513.1"
                     /translation="MKKLILLIAIIIIFVVAFLSSPYFKDFLDALDENKVYKIENIKT
                     TDINTLEEFKFFGRGIVSYNNQKI"
     gene            complement(23405..24562)
                     /locus_tag="PLB33_02980"
     CDS             complement(23405..24562)
                     /locus_tag="PLB33_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOG62514.1"
                     /translation="MIYLDNAATTFPKPSSVYKSVMRAMTVYGANPGRGSHAMAIEGA
                     RIIYETRELLAQLFNLDDPMKVIFTFNATDSLNIAIKGILNSGDHVVTTEMEHNSVLR
                     PIKELEKSGVENTIVKCSPDGTINLNDLESAIKENTKLIVTTHVSNLTGTIFPIEEIG
                     KICKKHNILYLLDASQSAGVLNIDLKKYNIDFLAAPGHKGLLGPQGTGILLINNETEM
                     KQLKEGGTGSRSSNLEHPDFYPDKLEAGTHNLPGIAGLNAGIKYILNRGIESIYSHEK
                     KLLEIFIAEIRKNPKIKIYGPECIELRSGVVPVNIDGMDSSEVANILDLEYKIAVRPG
                     LHCAPLAHKAIGTEKIGAVRFGVGPFNKSTDIMAAADALNKISEQIGRGVQ"
BASE COUNT         7370 a         4791 c         3459 g         8792 t
ORIGIN      
        1 gacatataat tttgaccggt ttcttcatca tttataggca gaccgtgagg atttataaag
       61 cttgccgata aaagaccaag ctctgacgct ttagcattca tcatgcttac aaaattatct
      121 tgggtttttc ctacatattc agctactgca gttgcacaat cgtttcctga tactatgagc
      181 attccctcta ccagggttct cagtttaatt gtttcacctg ctaaaatgcc aaaattgctg
      241 ccttccgtag ctgcggcatg tccgctgatt gtaacatcgt catcaagaga aatttcacct
      301 gcagaaactt tgtccatcat cacagtataa gtcatcaact tggaaatgct tgcaattgcc
      361 aactgttgat ttatattata atcatagagt atttctcctt ctaaatctcc aagcaaggca
      421 ccctttatgt tttcatttat attaatatct gcaaatacaa gagtatttgt aagcataatg
      481 attattaaac acaataaaag tgtttttttg attttcttca tgaaaatccc tccaatcaag
      541 atgctataat tatacaacaa tcttttgata tctgcattac taaatttaga aatttgttgg
      601 aaaatttggt aatttgtttt attttgaaga tttttcatat atccacttta aaatatcttc
      661 atatatgttt tctttatcta attcatttaa tatttcatgt ctatcatcct tataaagctt
      721 gattgtaaca tcggtaagct cttttttgaa actgtcatat aatcttttta catcttttcc
      781 aaagtttcct acaggatcct gctctccgga taataggatt acgggtaatg tttttggtat
      841 tttattcatg tattctttac cgtataaata cagcattcct ttaaacatgt aataaaaagc
      901 attcaaagta aaaataaaat ctatttttct atccttaaca taagcatcta ctgcttcttc
      961 gtccctgcta agccaatccg acttggtttt agcaggctca aaatatttat tgttgcctcc
     1021 gattgtcagc ttatttataa acttgcttct gtatctccat cctttaaaaa ctgccattaa
     1081 cttagaaatt atcagacctg cttttactga taaatatggc tgataagcgg ttcctacgag
     1141 taaggcacca ttcaaatcat tatatattga aatatactgt cttaccagaa atgaacccat
     1201 actgtggcct aatataaaat agggcaagcc ttcatattct tccttcaata tttctcttaa
     1261 ttttcgcata tctttggtta ctaacttatt tccgtcgcct tcaccgaaaa aaccgcggtc
     1321 ttcttcaaca agcactgaat ctccatgtcc aagatggtca tttcctgcag tcagtattcc
     1381 gttttcggcc atatagcgtg caaagcagtc gtacctatca atatgctcga gcatcccgtg
     1441 agcaatttga agcacacccc ttatttcagc atcaggcacc catttaaccg catgaatctt
     1501 tgatttttta tcggctgaat cataataaaa gtctttcttg attaccataa ttactccata
     1561 aatttaatat ataactatat aatttccccg ccatcatatg actctccgcc gcaatcaggc
     1621 ataataccat acctgattga ccaaacatgt ttatatttta atgcttggga atgtatttta
     1681 attatatctt cttcaggtac agtcttaatt actttggcag gtattcctgc ggcaacagaa
     1741 aaaggaggta ttttttggtt gttggtaact accgcacctg cagctataat gcttcccctt
     1801 cctataacag caccgtccat aataattgca cccataccga ttaagcagtg gtcttcaatt
     1861 ttacaggcat gaacagttgc attgtgcccg attgttacgt aatctccaat tattgccgga
     1921 taatcgtcgg caacatgtac aacgctgtta tcttgaatat ttgtatacct gcctatttct
     1981 atatagttta catctcctct tacaaccgta ttaaaccaaa tacttgaata ttcttttatt
     2041 gttaccttgc ctattatttg agcaccatcg gcaatgtatg ttttttcatc tattattggc
     2101 ttaatacctt caaattcttt aatcattatt tcctcccata caggacgttc ttcaattcta
     2161 tccaaagaat taccttcata gatgtccctt ttttattatc cgaaccaaat ctttatttct
     2221 ctttctgcac tttccttaga atctgagcca tgtactgaat tttcctgttt tgataaagca
     2281 aattttcccc tgattgaatc aggctcagca ttttttgggt ctgttgctcc gttaattttt
     2341 ctaaccctat caattacatt tttaccttca ataaccaatg cgcacacatt tcccctgata
     2401 atataagaaa taagttcatt gaaaaatggt ttgtccctgt gctcataata atgttcttct
     2461 gcaatttcaa cagatggctt aatcattttc atatcaatta tgttaaaatt cttttcataa
     2521 acagaaatta tttcacccat aagttttctt tcaattgcat ccggttttat aagcaccaat
     2581 gttctttctt ttcctaaaaa gtctgatatt tttgaaagca gctcatcaat attattaaca
     2641 tcaagcgaca gtgtttcaat aggacacatt cctgattgtt cacgattttt tatgtatgaa
     2701 accaattttt gatattcaaa cacaatagaa gtgtttttca aaatatccac agcattttga
     2761 gaaattaatt tagtgcttag ttctttgata tcagcatgtt tataaatcat tgcagccgcc
     2821 ttaccgacaa ctttatcagc cgcactagag cctttaagtt cgtctttgag ctcattaaaa
     2881 gcttcataaa caggtttaat tcctttattt tcacttgtaa aaattacttt tccatccttg
     2941 acaattacta ttgttttatt gtcattattc agtatttgtt ttgctaattc aatgtctttc
     3001 atatatactc caattcttcg ctgtgctcag gatgacaaac ataaggtttg tcaagcctat
     3061 gcaatggcta aagtttttgc ttcattgatt gtattcatta tagtatagta cagtaattgg
     3121 tgatttgcaa taataaaaac cggcataaac cctgagttaa gaatgctaac aaataaaatc
     3181 caaaatattt ggtttaaata ttttggattt atttgtattg tcctgctatc cttgaggcaa
     3241 ttgacatata ttatcacgac ttattaaagc cgggtttcat attcgaataa ttaattgttt
     3301 gacctctaga atatgctttc gatatcttca ttaagaatat cggttataaa catatgaccc
     3361 ggtgcatgag ttataactat ttcaggttta gcattttcaa ttgcagcttg cggtgtaact
     3421 ccacatgccc aaaataccgg tacttcgcct tcttttatta taactgcttc gccataatca
     3481 ggtttattga tatccttaat tcctatttcc gcagggtcgc cgtgatgtat cggagcacca
     3541 tgaacatgcg ggaatttccc tgttatttca tatgctttat ctacatcctc gggtttcata
     3601 ggtctcatgc ttacaacata tggtcctttg aatattccgg ctggtttagc ctctatattt
     3661 gttttataca taggaacatt acgacccatt gaaatatgtc tgatttcaat tccttctctt
     3721 atcattgctt cttcaaatga gaaactgcag ccgataagga aacaaacgaa atcgtctccc
     3781 caatattttt ctgcatttaa cacttcatct actcttactc cgtttttata tataaaatat
     3841 tttggaatat cggtaatgat gtttgcctta tctgcagtct ttgttgtagt ataacttccc
     3901 ggttcggtta tttcaagtat aggacatggt ttaggattat actccgcaaa taatttaaaa
     3961 tcatctgcgt attttttagg taatattacc agatttgctt gagcatatcc accgcacata
     4021 ccggatgtcg gttcttgaat tttttcttct cttattaatt gtcttacttc ttttggagaa
     4081 aaatcatgat atttattagc catttatctt atcctttcta taaatattga tagtattatt
     4141 taatctatct tgcacattta ttgtatcact gccaatcttt cgacctttta taggcatttt
     4201 ccatcaactt gtttctctcc acaataactt tatgatattt tcctaagcaa atccttccgt
     4261 tcgaatccgc acctacgata tcaagaagta tcttattgcc atctactttt tctaccgtaa
     4321 gtatcaatgt tattgtatca ccgaccaatg ttggatttat atgagaaatt tctaatcggt
     4381 ttccaacagt taaacattgc tgtggaatta aactatcaag cattttgcaa gaagcttcaa
     4441 cgatcatagt caaaagagcc ggagttgaaa caaggttttc tattgaagat gtatttgaaa
     4501 catatgcatc agaagcatta gtctgctttt gatatgttat tgaaagacct tcggtaattt
     4561 tatggtttat aatgtccacc tccatatatt catttgctta atcgtatctt agaattcatt
     4621 gaatctcttg aacatctacc acataattta ctccgtttat tactatattc attcttctta
     4681 cgggtccaat gttcacatat gccgcttttt caacaggtgc ttcaattact ctttgtgaat
     4741 ttgctatttg tttttcaatc tcagatagct tttcttcata ctctttatac aatttatgtg
     4801 cttcttccaa tgttatcttc tcaaatacta attgacagcc ttcgcccatt tgagcaagca
     4861 ttggtaagtc ggttgatata accgtagcaa tttttgtata accgcctgct gtttgtctgt
     4921 ctgccatcag tataatagga agtccgttag caggcacctg cactgaaccg aataaagcac
     4981 cgtcggaaat tatgtcggct ttatctatat gcttaatagt ttttccgtca agcctgtatc
     5041 ccattctatc tgcttctttg gttattttat atccttcggg acttaaaaaa gtatttatac
     5101 cttcttccgt aaaataatcg tcctggggac ctaataccac tcttacatta ccttttcttt
     5161 tgaattccgg tatatatttc ggatccatat attttccgta cactattttg tcggatactt
     5221 taatttccaa gatatcatca ttcattaatt ttcggccttt aaaaccgccg attcctgttt
     5281 ttaagtaagt tgatttgctg ttgtttaaga gaggtacttc aattgaaccg ctgaaagcta
     5341 tataggctct gagaccgctt gtaactactc caagagataa tacgtcaccc tttgtaactt
     5401 tatagctttc ccacattgaa atttctttgc cgtttagcat aggtttcatg tttgcgcctg
     5461 ttacagcaaa tgacatatcg cagccaaatt ccaattccgg tcctaataaa gtagcttcca
     5521 atacagcctc gccctcatta tttccaacca agaaattagc aactctatat gaaaaattgt
     5581 ccataacacc agcaacaggc attccgaatt tttggtaacc aatacgtcct aaatcttgta
     5641 ctgtagttaa aaacccgcct ttcttaactt ttacgctact cacgcaggtc acctccttcg
     5701 gaaattatca cttttacttc atatgtacct tcttctactt gttttttaat ttccaagtat
     5761 tcttcttcac ttactgatac atatcttaca taatcgcctg cactaagcat aaccggaggc
     5821 tctttgtcag gatcatataa ctttaacgga gtcctgccaa tcagctgcca cccacccggt
     5881 gtttctgacg gatacattcc tgtttgtgcc cctgcaattc ctacagagcc tgcaggaatt
     5941 tttgttcttg gtgaggacaa tctcggtgtg gctattttat tgctcatacc tcctaaatat
     6001 gtgaatccgg gagtaaatcc catcatataa accagataat ccgtacctgt atgaatttta
     6061 ataacctcat ctacagtaat attgttatgt tctgcaacaa agttaatatc aggtccatat
     6121 tctccgccat acacagtagg aaactccaca attcgaattt tttcatctga actttccact
     6181 tgcttgctac tcatagaatc aagagttgaa attagatcag aatactctat ctttaacggg
     6241 tcatacataa tcatcaaaga cctgtatgta ggcaacaatt caactattcc atcaatttta
     6301 gcttcatcta tcagttttac aacatttctg attttagcat tgatttcttt ggaaatttca
     6361 ttgccgaatt ccattactac agctttatct cctgcagaaa ggtattttat cttttcatac
     6421 atatcattat tcctttcatc aattcttgtt tatttccaca atgctgctat accggtaagt
     6481 gatcttaaac ctaagacagc tgttacagca acaactatcc atcctaatat aaataaccac
     6541 tgtgcatgtt tgtattcacc cacaatgtct tttctcttag aagctacaag tattgttcct
     6601 aatgtaagcg gtaaaataag tccgtttaat gagcctacaa taaccaatac ttttgcaggg
     6661 ctgcctacaa atatttgtat tgcggttgat gctactatga atgctatggt agtaatatta
     6721 acatggtcgc ctattggttt aattaatgtt cttaagaaag aaaccgatgt gaaagctgct
     6781 ccaacaacag aagttaatgc agcggcagcc ataaccattc cgaaaatttt atagcctaag
     6841 gtaccagctg ctattttgaa tgcatctgct gcagggttac ttgcgtctaa ggtaactcct
     6901 gttgaaacaa cacctaatac agctaggaat aatagtattc ttactatagt tgcaactccc
     6961 atacccatga atgctgattg attaactcta tcaaagtttt cttctcctac gattcctgca
     7021 tcaataagtc tgtgtccgcc tgagaaagaa atgtatcctc ctactgttcc tcctatcaag
     7081 gtcaatattt gcagataagg gaatgaactg ggaacgaatg tttcttttat tgcttgacca
     7141 accggtggct ttgatgaaat tgaaacatat aatatcaaaa taatcattaa tgctccgcag
     7201 aatgttgcaa atttatccat tgcatttcca acttgcttaa cagagaatat gattataccg
     7261 ataattcccg aaattgcaat agctacattt aagtctaatc caaacaaaac ttgtaatccc
     7321 aatccggctc cgccaacgtt accgatgttg aaagccaaac cacccattgc aaccaaaatt
     7381 gccactacat atcctaggcc ggggaatact ttatttgcaa tatcttgacc tcttgctttt
     7441 gatatacaaa taactctcca gatattaacc tgagcaatta aagaaaaaag aactgatact
     7501 aaaataacga atccgaatgt cggtcctaat tgtgccgtaa aagttgtagt ttgtgttaaa
     7561 aatccaggtc ctattgcaga tgttgccatc aaaaatgcag cacctataag gattcttttg
     7621 tcctgtgatt tatctctttc tgacataata ttaatctcct ttattattat tttagtcggt
     7681 taaattctta accgctatta tgttaatcgt atgtgtcttt tgtaagtaga tatacctttg
     7741 ttgttaaata aaatctttta aaggcattat ttgaataccc tcatcttcta aagcttttct
     7801 tattgttttt acaaattcga cagctttagg attatcacca tgtacacaaa ttgagtctgc
     7861 tttaatatca atatcttctc cggtgatagc ctgaactttg ccttctttaa ccattctaac
     7921 aacccttgta agtgcaagag tttcatcagt tatcatagag ccttccagtt tccttgaaac
     7981 cagagtccca tcggtattat aagctctgtc cgcaaatact tcatttgctg tcttaagacc
     8041 tactgccttg ccggccttta tcatttcgct gtttgcaaga cccatcaaaa ttaagtcttt
     8101 gtcaacttca taaattgcct ctgcaattgc catagccaat tcaggatctt ttgcagccat
     8161 gttgtataaa ccgccatgag ctttaacatg ctgcagctgt gttccacagc ttttcgcaaa
     8221 tgcccacaaa gcacctattt gatatttcac atagttcttt gcttcctctt ttgttacctt
     8281 catttctctt ctgccaaatc ccattaaatc agggaatccg ggatgtgcgc caatgcaagt
     8341 gtcttcttta atagcaaaat caactgtttt ttgcattacc atcgcatctc ctgcatgcca
     8401 tccgcatgca atatttgctg acgaaacgta ttttaggact tcagaatcca agcctagttt
     8461 ataatttcca aaactttcgc caacatcact gtttaaatct actttgtaca aaattaatca
     8521 cctccaaata aatattatgc aattaatatg ccaagattat ctttaataat aatctccata
     8581 tttcaacaaa aaataaagag taaataaata tttgcgtgcg aatatgcacg ttttgccgag
     8641 cgatagcgcc ctagttattg atgttctata tataattcta ccaaattatg cattattatc
     8701 tgcataaaag tatttttcta ttctcctttt aaaagcaaat ctgcttatac ctagcatttc
     8761 cgcagcttta gtatggttgt tgctgcaata tttaagagct agtttaatat attcagcttc
     8821 catttctgca acttcatttt ccaaagaaaa acctcctgaa agcagtttac tgctcatctc
     8881 atcttgactc ggctttgtaa attcattgcc tccgcctaag gcacttctaa tttctaaagg
     8941 caaatcgccg atatcgatat attcgttctc taccaatatt acaattcttt caataacatt
     9001 tttaagctcc ctgacattcc ctttccatga gtataataat agtgcttctt tggctctgtc
     9061 agtgaatcct tttatctttt tattaaattt acagttatat tcatataaaa aattatttgc
     9121 taatactaat atatcatctc ctctttcacg taaaagaggc agataaacag gcactacatt
     9181 caatctgtag tataagtcct ctctgaattc tttatcttct atggcttttt ccaagttttt
     9241 atttgtagcg gcaataattc tgatatctac ttcaatatcc tctagtccac cgaccctttt
     9301 aaattttctt tcatctaaaa acctcaatag tttaggctgt acttccatgg gcatttctgc
     9361 aatttcatcc aaaaataccg tacttccatc agctatttca agcaatccct tttttctgtt
     9421 gtttgcaccg gtgaacgcat ttttttcaaa tccgaaaagt tcactttcta taagctgctg
     9481 aggaatagca ccgcaattaa gtttcagcat ggcagatcct ctccttatgc tgtgattatg
     9541 aatataagaa gctacaagat ctttgcctgt accggtttca ccccttataa ggacagttac
     9601 attgtcgttt tttgataata tatcaattct attcataact tcattcataa gaggatggtt
     9661 ccctattata attttcttat ttaattcttt ctccttttct ttctccaaca aataaatttt
     9721 gttttgcaat tttaaatttt ctgtaattct ttttattaaa atttctacat caatgaggtc
     9781 aaagggtttg ttaatatagt caaaagcgcc gagttttatt gctttaacag cagtgttgat
     9841 atcaccatag gcagtcataa taataacctc gacttttttg tctatttttt ttattttagg
     9901 caaaatatcc aatccgttct cacctgctaa acgcatgtct aaaaaaacta tctgaggttt
     9961 atattctttt accttttcaa gagcaatatg accggttgca gcagtactta tgctatatcc
    10021 caaatctttc agtgcttctt ccaaagataa tcgtatagtc tcttcgtcgt caactatcaa
    10081 tattctaatt ttcattacta cctccattaa tgggaaacct gattattact ttagttcctt
    10141 catttactgc actctgaata tctatttctc cccgattgcc cattaccaat tcatatacta
    10201 cagataaccc aagtcctgtt ccttgagggt ttgtagtaaa aaaaggatta aatacctttg
    10261 ctaaatcttg tgattgtatc ccacatccat tatcatgaaa aataacttca ataaattttt
    10321 ctttttgtga gcaattatta aaagaaatat ttaaagtacc tcctttatcc atagcaaaaa
    10381 aagcattatt gataatattg atgaatatct gctcagtttg agccttgtcc acatatgcaa
    10441 cagtattttt gcagttatta tcaatattta tttctatatt attttttttg gcagtctccc
    10501 cgattagatt taacgactta tctaaaactc ccattaaatc tgttttttca atattaggaa
    10561 aacttggctt agcaaaattc aataaatcag taatcaaatt attaagtctg tcaatttcaa
    10621 aaagtactcc gttaagtaat tttttattag aagctatcat atcgttatcc tctttgcata
    10681 acctgttttt taaaacttgg atacttgttt tcatgcctgc caagggattt cgtacttcat
    10741 gagcaatacc tgatgcgaat tggcctatgg tagttaatct atccaatgtg ctcatgctgt
    10801 tttcgattct ttttctttcg gtaatatcgt taaaattgca tatagctccg ctgatagacc
    10861 catctcctcc tatcaaaaga gaagttgtaa catctatata ttttttattt ccatgctcat
    10921 catcaaaagc aaggatttta tttattttca tctttgtatt taatgtttcc attatttgtt
    10981 catgcaaaca ttccttgaca agaccgattt tctcggctgc gggaatcagt aaattttctg
    11041 cggtctgatt tattgatatt acactgccat gctggtcagc agttactatt cctgctgata
    11101 cgtttcttaa aatatcttcg ttgaatttgg ttgcttcaat taatctgtct gtattgtttt
    11161 gcacagtatc gagcatattg ttaaaagcat cagccaatgt tcctatttca tcatttctgt
    11221 ctatttctat cctatcttta taaattccgt cagcaacgat tttattacaa tgttcagcca
    11281 gcattttaat cggttttgat atattgtgcg taatgaatat tgatgcatgc atagaaaata
    11341 caagggcaat taatgcaact aagattatat attgttgact ttccattact tcaattaata
    11401 agttatctct atttaagccg tatgctatta cccaattcca cttatcatac ttttgatatg
    11461 taaaaatata attgcctaca gatattgact ccggtccttt atttaattct tcattcataa
    11521 attcttcaat gaagttatta ttcaggacag tatttaacaa aatttcatca tcatcatcgc
    11581 tgaaaataat caaattgttt ttgcccaggt ctttataaaa attaataata aaatcttttg
    11641 ctccaatttc atcctttatg tcatcattaa ctttttcaat agtaagaatc atctcttcta
    11701 aattacggta tgaaatattt atatgattat ccaacaataa attatatcca ttaacaacaa
    11761 atacaataac aattattaaa attgatacca tcaatataat aataaaagga atgagtatct
    11821 ttttttcaat actgtttctc ataatttttg ccttcccttt ttacgagcgt attcacatga
    11881 ttataactga aatcaaaatg ctttctctaa atcatctcta ctatgattac acaattacca
    11941 taataaaaga attcaacaat aaataataga atcccgccta tatatcggaa atgtagcatt
    12001 acactacact tccaataaga ggattctatt atattgtacc ataaagtaag actaaagaaa
    12061 atatttttat agaataacaa taccttgtta ttttgttatt ttttccaaac ctctcatgta
    12121 atccctcaag acattaggaa tagtcactgt accatcttca ttctgataat tttcaagaat
    12181 agcggcaaat gtccttccca cagcaagtcc ggagccgttt agggtatgaa tatactctaa
    12241 tttacctttt tctgcaggtc taaacttgat attggctctt ctagcttgaa aatcttcaaa
    12301 attcgagcaa gaagaaattt caacatatct gttgtagctt ggcatccata cctccaaatc
    12361 ataggttttg gctgagctga aaccgatatc tcccgtacat agctctacaa ccctgtaagg
    12421 aagacctaat ccctttaaaa tttcttcagc aaaatttgtc agtatttcaa gctggttata
    12481 agaatcttca ggtttggcat acataaccat ttcaacctta tcaaattgat gatttcggat
    12541 taggcctctt gtatctctcc cggcagaacc tgcttctgcc ctgaaacatg gcgtataagc
    12601 agtcatatac attggaaggt ccttttcatc cagcacttca tttcggtata tatttgttac
    12661 gggaacttca gcagtaggaa ttaaataata ttccctttga ttaagcttaa acatgtcttc
    12721 ttcaaattta ggaagctgtc ctgttcctat catactgtcc ctatttacca taaagggagg
    12781 agaaatttcc gtaaagccgt ggtctaaggt gtgcttgtca agcatatatg caataattgc
    12841 cctttccaat cttgctcctt ctcccttgaa cattgaaaaa cgagtgcccg taatttttgt
    12901 tgccctttca aaatcaagaa tatccaagtc tacgcctaaa tcccagtgag cctttaattc
    12961 aaaatcaaac tttctcggct ccccccatct tcttatttcc ttgttgtctg catctgaagc
    13021 accgacagtt atgtcgggat ttggagtgtt tggaatattc aacagctctt cttcgatttt
    13081 ctcatcaatt tcttttacct ggccatccag aacctttact ttttccgcca attctttcat
    13141 tttttgaaga atttccgtca catcttttcc ttcttttttg agaagaggaa cctgtttaga
    13201 tactttgttc tgctctgcct tcatgttctc cacttcaaca agtttttctc tcctcgcttt
    13261 gtctaatgca agaaccttat ccaaactaag ttcggaattt cttcttttca acagttcttc
    13321 cacctcatta gggttgtttc ttattctttt gatatctaac attttttacc tccataaata
    13381 aaaactcccg cctctttaga gacgagagat actcgcggtg ccaccctaat tattatacta
    13441 atgggggaca ttccttcatc cccggagact ttccctaaaa aaggtgtgtc cccataccat
    13501 aatataatca ctttaagaag ttaacgcctt caacgtctaa gttttttacc cttagatact
    13561 ccggaacgga ttcaacaact attataccgg ctctcaccac ccgtcggctc tcttaaataa
    13621 gtcgctgttt actgctttcc ttcattgttt atatggataa tataataaca ttataacttt
    13681 gtcaagcttt atttaatgga ttgaaggcga tattatgcac aaggcttctg acacatcatc
    13741 atttttattg taatacatat gattgttgtt ttcaggaaca attatgctgt ctccctttga
    13801 tattgtgtat attttttctc ctacatgaca ctctatttcg ccctgtgtaa caaaaatata
    13861 ctctttagat tcatgtgtta tactatgatt gcttatccat gtttttggtt tcattttaaa
    13921 cagaaaggct tcaaactccg attgtttatg actatttttc aatattggcg taataaactc
    13981 aagttccaca ttaatattgt tcaatttaac tttagttctt tcttcttttg gtattgttat
    14041 aacatcattg gaagattcct ccgaaaataa tactgaaaga ggtacatcca atgcatcaga
    14101 cagtcttttt aataccgcta gagaaggaga tattaaatct ctttctattt gtgatatata
    14161 tcctgatgtt acgttaattt tctctgacaa ttcaacaatc gtcatatttt tattctttct
    14221 aatattcctg atttttttcc ctatcattat aataccccct gtactgctta tactaatatt
    14281 atacactaaa taaaacttat gtcacgtaaa ttttcacatt ttataccatt aaatggtata
    14341 ttaaaatgca ttgcagtttc tgctcttgtt ttaatgatag tcagcaaaga caaatatttt
    14401 acttttattg tagaaagttg gatttttgct tttcatataa tactaattat gatataatta
    14461 tctattaata ctttgtaatt attcaattat atgcataagg ggatatgaaa tggctgataa
    14521 cagagtgtat ttggaagaaa taaaatattt ggatgaaatt ataaatttgt taaaaaataa
    14581 gttggaatat gaaaaaaacc aacttgaaaa taaaaaatcc gatttaattg agtcaagaag
    14641 agaaatgtgg gaaaatacaa cccattcatc tgccgatttt gataaactaa ctgaccttaa
    14701 tcagtatttg agtgcccttc aggctcaaac tttcacctat gccgaacttg caaaacgtat
    14761 tttaaagcat gaaaagatgc ttgacagccc ttattttgca agaattgatt tcaccgaaga
    14821 aggatatgat gatattgaaa aaatatatat aggtcttttt aatttaatgg acgatgaaac
    14881 ccatgaaatt aaaatctatg attggcgtgc acccatatcc ggcctatatt accgaaatga
    14941 agtaggtcct gtagaataca aagctcctgc cggattaata aagggctatg tttcattaaa
    15001 acggcaattc aaaattacaa aaggtaaaat ggaatacttc attgacagca acattaacat
    15061 ccttgatgaa atgctgatgg ttgctctatc aaaaaatatg acctccaaaa tgaagacaat
    15121 agtagaaaca atacaaaaac aacaggactt aataataaga gacctgtata acgacttatt
    15181 ggttgtacaa ggggttgcag gcagcggcaa gtcatcggtg gcgctgcaca gaattgcata
    15241 tttgatgtat caagggttaa acttaaaact aagagccaat aatgttatag ttatttcacc
    15301 taatccgtta ttctcaaaat atatatcaaa tgttctacct gaattgggag aagaaagtat
    15361 caatgaatat acttttgaaa atatatttca taaactattt ggaaataaac tatccatgaa
    15421 atcaaaaagt gaaaactttg aaaatattat tactgcagaa actgaagaac aaaggaattt
    15481 tctgaggtca tacgatgatt ttaaaggctc atttgttttt tcaaaaatta tagatagatt
    15541 tatagaatat tatgagcata agcttataaa ttttgaagat gtttattaca acggaaaaat
    15601 aatagaaaaa cgcgagcttg caaaagcttt tctgttaagc ggaaaacttg atatgcctac
    15661 agccaaaaaa cttaaaataa ttgaaagccg aatattggaa aaggttcgcg aaaataggat
    15721 tgaaaggcgt aacaaaattg aaaaagcagt aaataaatta aataatcatg aatttgaaat
    15781 aatgtctttt acaagattgc tatcagctaa agaaacctta gctttaacgg aaagattgaa
    15841 gaaattcact gaatttgatg ttttcgagct ttataaaaag ctgtttgcag atattatttt
    15901 atttaaaaaa atttcgaaag gtcttgaact tccggagaat attgatgaaa taataagtta
    15961 tacaaataca actttaagcg attcttacaa tattccttat gctgatggaa tagctctcac
    16021 ttatatgaaa atcaaagcag agggttgtga tctgtttagc gatataaaac aagttgttgt
    16081 tgatgaggcc caggattatt accccattca ttatttaatt ttaaaaaatt tatataaata
    16141 tgcaagattt acaatagtag gcgatataaa tcaatcaata gaaaaaagca acgatttgtc
    16201 cttatatgat gatattattt ctattttcaa ttttgaaaag agcagtaagg tttttctgaa
    16261 taaaagttac agaagttcat atgaaatagg caagttcagt gcaaaattat cgggagatga
    16321 aaactatgcg gaatttttta aaagaaatga agaagagcct gttattttca aatttaaaga
    16381 cattaacaac ctccgggatg aaattataaa aactattgag aaatataaaa ctgaaggatt
    16441 tagctcaatt gcaataatat gtaaagatag aaaggaagcc tcagacttgt attttaaact
    16501 ggctaataaa ataaaaataa aattgataga ctatttagat tatgacagtg ttttggaagg
    16561 taccttaata cttcctgttt atttggcaaa aggtctggaa tttgatgctg ttattgtata
    16621 tgaagcaaat gataaacatt ataataacga attcgataaa aaacttttat atgtcgcatg
    16681 tacaagagca cttcacagat tatcactgtt ttatacggga aatttaacaa aatttttgga
    16741 ctaaaaaatc cgcttaaccc ttaagcggat ttgcttatat atggtcatgt cataaagcgg
    16801 gtgatgggaa tcgaacccac acagccggct tgggaagccg gaactctgcc actaagttac
    16861 acccgcaata aatggagata aggggtctcg aacccctggc ctcttgaatg ccattcaagc
    16921 actctcccaa ctgagctata tccccaaaag tgtatgtatc ttaattttaa gaaaaaatac
    16981 tatatataat ttaatatatt ttttttcatt tgtaaagtta atcttttaat aaaaaagcta
    17041 ctgaggggat tcgaacccct gacctactga ttacgaatca gttgcgctgc ctgctgcgcc
    17101 acagtagcat gtatataatt taatactttt ttggcaattt gtaaacagta aattttgtaa
    17161 gactaaattt ccgggaaata atcgtaacat gagaggttgt aacacatata ttataatata
    17221 taaattttat atagaggaga gtatatatgg acgattacaa tcagtattat gataccatga
    17281 acgattatat gtattatgat gatattaata aagatgtata cagcagtcaa tacgatatgt
    17341 atgacaggag attcgacaac aggttcgaca acaagttcga cagaagattc gatagaagat
    17401 atgacagatt tttttatgac ccgatgaggt attatcgatt cccattctgt gacaggtacg
    17461 gaagatgtga aaatcctatt tggtggctgt tctggccctt cttttttcaa taatcagtat
    17521 gacaaaggga cgctctgcgt ccctttaaaa tttgcgactt cccggtttta ttggctttga
    17581 gcccatttca catagggggc aatttttatc atcataggtt tcaaagaata attctatcga
    17641 gctgtaaaca ggaaattgaa tttcacatac ttttctatct acaatacatc cgattcctac
    17701 tactataccg cctgcctctt caagaacctt ggcagtttcc atggatgatt tcccggttgt
    17761 tacaacatcc tccattatta aaactctgtc accgggttgt atttcaaaac cccttctaag
    17821 tgtcattaca ttattttctc tctcagtaaa tattgttttt atacctaatt gtctgcctag
    17881 ctcataagcg gctgttattc ctcccatagc aggtccgaca ataacatcca aatttaatga
    17941 ttttactttt tcatatacaa gagaaataac tttttctgcc ttgtccggat attgcagaag
    18001 tttagcacat tgacaatatt tgttgctgtg cttgccggat gaaagcaaga aatgaccttc
    18061 aagcaatgca ccgcattctt tcaatatttc aattacatct atcatattac cgcctcctgt
    18121 ggctctaaat aatccctctt atttcttcta gggatttaat attttcattg tccataaatt
    18181 cttccaaatc attgagaatg tctactgcaa tatcaggttt aataaagttc gctgttccta
    18241 tttgcaccaa tgttgctcct gccataatga attcaattac atcgattgca ttcattatac
    18301 ctccaagacc gcaaatggga atattaacca tttttgcagc ttcatgtacc attcgaagag
    18361 caataggttt tacacatggt cctgaaagtc ctgcataaac attctcaaat acggcttttc
    18421 tgttttttat atcaattgcc atagcttgaa ttgtattaat taatgaaatt gcatctgctc
    18481 ctgccgactc acatgctaca gccatatccg gaatattttc cgcatttggt gatagtttaa
    18541 ccataagagg ctttttacat atcttacgaa ctcttgatac tatatcataa gcaacggaag
    18601 atttcactcc aaaagctatg cccccgcttt taacattcgg acaggaaatg ttaagctcta
    18661 cagcatccac atttgtatca ttcagcttat atatgccttc taaataatct tcctcgcaat
    18721 gtcctccaag attggcaaaa ataatagtat tatatgtttt catcctggga agttcttctg
    18781 ttataaattt gtcaacaccc gggttttcaa gtcctatgct gttcataagc cccattggag
    18841 tttcccagag tctgattcct ttattcccgc tccttggagt taatgtaagg cctttgctgc
    18901 aaattccgcc aagaattgat aaatcgtaaa tttgagaaaa ttcttctccg aaaccaaaag
    18961 ttcctgatgc cgctattatg gggtttttaa atttaagatc cattgcttcg gtttttaaat
    19021 ttgccatatt tctcctctat attaattctt cactgtcaaa tacaggacct tccttgcaag
    19081 ctcttttatt gcccgtcttc gtttcgaccg tacaccccaa acaagctccc ataccgcatg
    19141 ccatccgcct ttccaacgat acatagcatt taattttttt gtccctgcat atagttgcaa
    19201 tcttactcat cattatttcc ggcccgcagg ttacaacaac attataatca tcataagaaa
    19261 tataatccgt tacgtaacct ttttctccaa agcttccatc ctccgtaaca acgatgcatc
    19321 tgcgggctga agatttaaat tcatcaatta tatacatatt ttctttatcc ctgtatccta
    19381 aataaacatc tgctttttta cctaagactt tacataaata caatagtgga gcaacaccta
    19441 tgcctcctcc tattactgca actctgccta acagtttatc cggctcaaag ccctttccca
    19501 agggaccgaa tatctgaatt tcgtcatttt ttcgcaggct gcttattttt tttgttcctt
    19561 gcccttccag tctatataaa aatgaaacaa gattctcatt tacatcatta acgcttatgg
    19621 gtctcggaag caaaaatgca ttatcagtgg ttttcagcat gaaaaattgt ccgggtttta
    19681 tttccgaatc aaattttacc gaaagcctat aaatattttt ggaaatttgc ccgttttccg
    19741 caacagcggt tactaagacc ttcattattt tctccaattg cttaccttta cttcttatca
    19801 taagccgtat cgcagtatac gcacctgtat attccttttt ccctgtcagc aagtctgaat
    19861 ttatgaacta tttcctgctc tattgatgtt atgcatctcg ggtttttaca tttaactaca
    19921 ttttcaactt cctgaggcaa ttctaaatta attttgtcta ttataacaga attgtctatt
    19981 acattgattg ttatgtttgg gtcaataaaa ccaagcacct taaggtccat atccagattg
    20041 ttttctactt ttattatatc tttttttccg tatttcttgc tttttgcatt tttaattatg
    20101 gcaacactgc aatccatctt atcaagattc aaataatgat atatttccat tgccttgcct
    20161 gccttaatat ggtcaattac caatcctttt tctacactgt ctatattaag cattattcca
    20221 cccccaatag tttcaatatt aaagccattc ttacatacat accgcattta gcctgtataa
    20281 aatatgctgc tctcggatca ttgtcaactt ctaccgatat ttcattgact ctaggaaggg
    20341 gatgcataag aatcatatct ttttttgcat ttgacatttt tttattgtct acaatatagc
    20401 tgtcctttaa tcttatatag tccgcttcat tgaaaaatct ttctttttga actcttgtca
    20461 tataaagaac atccaactcg ccaataacat tttctagtct ttccacttcc ataaactcaa
    20521 taccgttttt cattaatatt tcttctctga tataatccgg cactctcaat tcatccggtg
    20581 atataagaat aaattttata ttttcgtatc ttgataatgc ttttatcaag gaatgaacgg
    20641 tacgtccgaa tttcaagtca ccacaaagac caatcgtaaa attatttatt ttacctttta
    20701 atgtttgaat ggtaagcagg tctgtcaatg tttgagtcgg atgctgatga cccccgtctc
    20761 ctgcatttat tacaggaata tttgctttca ttgcagctac catgggagca ccctcttttg
    20821 gatgtcgcat tgctgctata tctgcataac atgcaactgt ccttatagta tcagcaacac
    20881 tttctccctt tgcagcagag cttgattcag cagatgaaaa acccataaca cttcctccga
    20941 gccttaacat tgccgattca aagctcagcc ttgttcttgt gcttggttcg taaaataaag
    21001 tcgccagtac ttttccctta cacttatctg cataggctgc aggatcgtgc ataattctat
    21061 ctgccagatt taagatatcg tccagctctg caagtgataa atccattggt tcgattaagc
    21121 ttcttccttt taacattttt cctccttttt agcctctcag gactaattta aagtgtnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa aatcttcctt gcgcacagca
    21301 aaggaagacc taattaataa gctcatattt tccttcccag cctctctgtg ccagattaaa
    21361 ggttattgac ttcaactaat attattatat ttttacctga ctgtcaatat ttttttactg
    21421 ccggtcagaa gtttcatttt caacttgata tataattttt gttatccttc tttctacttc
    21481 gcttctggga agccatacct gtcttttgca ggtagtacat ttaattctaa aatctgcgcc
    21541 tattctcagt atttcccaat cataacctcc gcagggatgt tttttcttta attttaccac
    21601 atcccctaca tttaatttca ttggcatagc aatctcctta tattataatt gaatttgtta
    21661 gtttattata ggtgcaagct gcaaaatcgg cttgcttcac atcgatacaa tgaatacttc
    21721 ggggcaaaga cccaagtctt tgcttcatta atcgaattca gtttataata catattttag
    21781 acaattccaa tctttaatat gctctcaatt tcaaagaaat ccaaacatgt tccacgtgaa
    21841 acaatttttg ttttccgctg aggtcatacc cttaataaac aagtttcatt ttttccctgt
    21901 taattttcgt aaaaaccttt atatgacaaa taattaagta taatattgtt ttcataaaat
    21961 attttgcttg atttagaatt caaaatcgat tgagctaatg gtttgtcatg cataaaactt
    22021 attaaggggg tagctagggc gaaaatcaaa tataaaagca taacactttt tactattccc
    22081 attagtgctc caaacagtct gttggctaga ttcaataaag gcagtttcat tactgtattt
    22141 acaatgaatg atagaataag cagtatagca tatattgata agaaggttac aataaagctt
    22201 atcgacctta ttattaaaag acttaaatta tctacaaaaa tagcaaaggt atccgacgtt
    22261 tcgcttgcca ttatgttatt tacaaacttc tgcagctcaa cgggaatttt actaagatta
    22321 ttaaatgcgc ttggtgaaag tttcacttca aaataatcat gcagttttac aaaaagcttt
    22381 gtattgttta gtaaaaaatc ttcaccaata taataaaacc ttttgcttaa gaaaaaagaa
    22441 attatcatgc ctaatgtatc aaagaccgtt aatatgaatc ccttcctgta cccttggaaa
    22501 aacaagtatc ctgtaaatat tagtataata acatcaatta tcacgctagt tccccctttt
    22561 aatttatttt atttgtcctg acataatctt gtttttaaat agaatataaa tggtatttcc
    22621 ttcaatcaag aaatgttcaa aactttcaaa ttcctcgaat attttatcgc ttttattact
    22681 gtgaagcaaa taaatagaat tattattata aaccaaagtt ttattatcaa ctattttcaa
    22741 cttgtggaca ccgaaagggg ctgcttgtat ttcaataacc ttaccttcaa agttataaga
    22801 tattaattct gtagattcgc ccttttcaaa caatatatta attctatcct gtaatatttc
    22861 ataatcgagt attttattat atatactgtt cttccacatt aattttccgt tattattata
    22921 aaaatatata ttttctgttc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23041 ntattttttg attattatat gatactatac ccctgccaaa aaacttaaac tcttcaagtg
    23101 tattaatatc tgttgtttta atattttcaa tcttataaac cttattttca tccagtgcat
    23161 ccaaaaaatc tttaaaataa ggagaagaaa gaaaggcaac tacaaaaatt ataattattg
    23221 caattaataa tattaacttt ttcatatatc accctgtaaa attttgttaa tcaattatac
    23281 caaacttttt attaaattac attaattaat tcttaaattt gtgatatttc cacaataaaa
    23341 gcatgtttat ccgtttctgg acatttgcaa ttaaggtata ttcctccaat ttgaggacat
    23401 tttcttattg cacccccctg cctatttgtt cagaaatctt atttaatgca tctgcagcag
    23461 ccataatatc cgtgctttta ttaaaaggac ctactccaaa tctgacggcg cctatttttt
    23521 ctgttccaat agccttgtga gctagcggag cacaatggag accgggtctt actgcaattt
    23581 tatattccaa atctaaaata tttgcaacct cggaagaatc cattccatca atgttaacag
    23641 gaacaacacc gcttcttagt tcaatgcatt ccggaccgta tattttgatt ttagggtttt
    23701 tccttatttc tgcaatgaat atttcaagaa gttttttttc gtggctgtaa atcgattcta
    23761 tgcctctgtt taaaatgtat ttaattccgg catttaatcc ggcaataccc ggcaagttat
    23821 gtgtgcctgc ttctaattta tcgggataaa aatccggatg ctctaaattg cttgaccggc
    23881 tgccggtgcc gccttctttt aattgtttca tttctgtttc gttatttatc aaaagaattc
    23941 ccgtcccttg aggacctaac aatcccttat gaccgggcgc tgccagaaaa tcaatattgt
    24001 atttttttaa atctatattt aaaactcctg cgctttgtga agcatccaat agatacagta
    24061 tattatgttt tttacatatt tttccaattt cttcaatggg aaaaatggta cctgttagat
    24121 ttgaaacatg agttgtaacg attaatttag tattttcttt tattgcactt tccaaatcgt
    24181 ttaaatttat tgttccgtcc ggagaacatt ttacaattgt attttctact cctgattttt
    24241 ccaattcctt aataggtctt aaaacagagt tatgctccat ttcagtagta acaacatggt
    24301 ctcccgaatt taaaataccc tttattgcaa tgtttaaact gtctgtcgca ttaaaagtaa
    24361 atattacttt catcgggtca tctaaattaa aaagctgggc taagagctct cttgtttcat
    24421 aaataattct ggcaccttca attgccattg catggcttcc tctgccggga tttgctccgt
    24481 agacggtcat tgctctcatt acgcttttat atactgatga aggcttagga aatgttgtag
    24541 ctgcattatc taaatatatc ataatatcac ctgctgtttt tattttcaat ttcattatat
    24601 gtaaattaca gc
//
LOCUS       DAOSHP010000016        23773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1685_length_23773_cov_9.174045, whole genome shotgun sequence.
ACCESSION   DAOSHP010000016 DAOSHP010000000
VERSION     DAOSHP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23773
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_23773_cov_9.174045"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLB33_02985"
     CDS             complement(<1..287)
                     /locus_tag="PLB33_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOG62515.1"
                     /translation="MNTNKNILRLVFFPLLILYYETILKAFTYSKIFNIGYVYMCLFS
                     IPLGLLFYLLSTGFNEKTNKIIFFSIVSFLTLYYGTQLIYYRIFYTFTSFYS"
     gene            complement(395..1306)
                     /locus_tag="PLB33_02990"
     CDS             complement(395..1306)
                     /locus_tag="PLB33_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOG62516.1"
                     /translation="MKIIIHKKYLDIVSEKFPDIEFYTEIDECPEAEALIGEPMDFVP
                     EKLDKMPNIRWLQSFRAGYDTLDLDYMKKRNITFCNGKDIYSIPIAEDVVCKILMHNT
                     NALKFLRSQREHKWEPRQKRTEFHGQTVGIIGTGSIATEIAKRLQGFGVKIIGYKRTP
                     VSAMAYFDEILSGKKGLEYLMSNSDYIIVTVDLNKDTYHMINKDTLKFMKESASIINI
                     ARGSIINQKDLTEALKNKSISYAGLDVFEVEPLPEDDELWDLENVYITPHASGIVKEN
                     KKRLAELIVANIQRFIEHEKLANVVVK"
     gene            complement(1413..2273)
                     /locus_tag="PLB33_02995"
     CDS             complement(1413..2273)
                     /locus_tag="PLB33_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG62517.1"
                     /translation="MKYKEIISEIKSVRLYKKEEVDSKLLKELKQYYEQGKRLVDDIE
                     VGVIRRSRDEVFDKLKDSAGYNNRMIEAPHYLIFLSEEKDHYIENAGYRAQDIMLKAW
                     SLGIASCWITFKDGEEIKKKLGIDSDKKLVALIALGYEDPQNKVLYEKGSKYNTSSNV
                     KLVEDNVSERLGIRDLVFSKEWGEKADPDELVNYGLLEGFFYGILAPSYKNRQPWRFI
                     VDNGTVVLAVKKDIYVTEYEEKIDTAVIMLYFDAIIESTLYDIKWKFGKPEKDYKVPD
                     DYKIVAYCIV"
     gene            complement(2347..3171)
                     /locus_tag="PLB33_03000"
     CDS             complement(2347..3171)
                     /locus_tag="PLB33_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG62518.1"
                     /translation="MEANLVVDSCVDFNEDTKDIPRVPFRIIIDEEEIVDENTDILEL
                     IRKMKKSRTQIKTACAPPEDFMNKIKVQANNFIVTISSQLSGSYNSAVMAMETIKEKF
                     PESFIHIFDSKTAAAGESLLALKVKQLIEENLNPHEIIEQTEKYISKMNTLFILESLD
                     NLAKNGRISNFKATLANIMNIFPIMGDDGEGRIVLKEKVRGRKKAFSRLVDMLAEYNI
                     DFESTVLGITHVNCLEKAENLRDAIKLKYNFKDIKIFSSAGLSTIYADDGGIVIAF"
     gene            complement(3235..3474)
                     /locus_tag="PLB33_03005"
     CDS             complement(3235..3474)
                     /locus_tag="PLB33_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG62519.1"
                     /translation="MHYIYIVECRDKTLYTGYTTDLDKRIKTHNAKKGAKYTRGRTPV
                     ILKYYEEFNNKADAVKRESQIKKLKRAKKLILLNK"
     gene            3638..4330
                     /locus_tag="PLB33_03010"
     CDS             3638..4330
                     /locus_tag="PLB33_03010"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOG62520.1"
                     /translation="MRIGIIGAMDIEVEELINSMDDIKKETISRIDFYEGKLQEKDVV
                     VAKCGVGKVHAAICTQTMILKYAPDIIINTGVAGSLSPDLNIADIVISDGVVQHDFDI
                     SPFGKPVGLITGIDLIKIPCNEELVKQLVKAAEVLEDTNIAVGTVASGDQFICSKEKK
                     DYIVNNFNALCTEMEGASIGHVCFINDVNFCIVRAMSDKADGTANMDFDVFVKIAVAK
                     SIKLVNNFLKQL"
     gene            complement(4371..5261)
                     /locus_tag="PLB33_03015"
     CDS             complement(4371..5261)
                     /locus_tag="PLB33_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOG62521.1"
                     /translation="MNRYFVIYNPSSGKELAAYKVFGAAETLMNVEDAEFLFYATKKK
                     GDGEEAAKRACQYGYEMIIACGGDGTVNEVVNGMMKCCYRPKLAIMPAGTVNDFGEQL
                     NLPKAQENFASLIMQRKFKTIDVGRVNEGYFVNVVGGGAFTTISHKVTIDAKTILGKY
                     AYYYRAAVKVPDELEVCENINYIIDGKQYDYKTLLFLVLNSAGAGGFRYLCPKAELDD
                     GLLDIVIFEKTSNAELFQIFTKVFNGQHITHPKVHYYQAENIKIIPEKELLIDADGDP
                     AGYAPAEISSVHNAIDILVP"
     gene            complement(5376..>6035)
                     /locus_tag="PLB33_03020"
     CDS             complement(5376..>6035)
                     /locus_tag="PLB33_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOG62522.1"
                     /translation="GDAVIDLQTRFFLDWSHASKQELMFLPKYFPDNRIQGKVGIQII
                     SSGPDKSDEVIKSNYVKMINSAKKSILIQTPYFIPDASVFEAIKIAAASGVDVRIMIP
                     CKPDHPFVYWATYWYCGGLLKYGVKVYIYEYGFLHAKTLVIDGTVASVGTANFDVRSF
                     KLNFECNAIIYDTKTSQMLYDIFTDDLNYSMELTRELYLERGILIRFKESVCRLLAPL
                     L"
     assembly_gap    6036..6135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6136..6496)
                     /locus_tag="PLB33_03025"
     CDS             complement(<6136..6496)
                     /locus_tag="PLB33_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOG62523.1"
                     /translation="MELFNSISLIYIVNICMAMIVIFLERKDPTATLAWVLVLLIFPG
                     FGFLLYLLLAQNFSRKQLFIMKVYAKKTFGDYIKVQKELFSTGGLVFNDKNIENYKDL
                     IKMNLFYYGFSYTQNNEI"
     gene            6627..>7491
                     /locus_tag="PLB33_03030"
     CDS             6627..>7491
                     /locus_tag="PLB33_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG62524.1"
                     /translation="MKKPSKYWILAGTFFTSVSSIIIRFSEAPALVISAYRMLFTCMM
                     LFLPVYIKNRNEFKSLNKSNFIMCVISGIFLAFHFASWIKSIQMTTIANSTVLVSCSP
                     IFVAAINYYLFKEKITYKMIVGISMSLLGTIIIGMGSSQGNESSMMLGNILAFMGAIF
                     VAGYLVIGGFVRRTVSAGVYVFIVYFVSAIVLFIMCFVTGIPVYPYPAKEYMLFFLLG
                     FFSSILGHTAYNYLMKYYSSTLISVSTLMEPIFASLMAMFIFSEIPSLYTVVGGIIII
                     GGIYYYLMTQNT"
     assembly_gap    7492..7591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7619..9220)
                     /locus_tag="PLB33_03035"
     CDS             complement(7619..9220)
                     /locus_tag="PLB33_03035"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOG62525.1"
                     /translation="MPKYIFVTGGVVSSLGKGITAASLGRLLKSRGLKVTLQKFDPYL
                     NVDPGMMSPYQHGEVYVTDDGAETDLDLGHYERIVDIDLTQYSSITSGKIYWAVLNKE
                     RRGDYHGGTVQVIPHVTNEIKCMLKRIASVEDVDIVITEIGGTVGDIESQPFLEAIRQ
                     IRYEVGRENVLYIHVTLLPYLSMAGELKTKPTQHSVKELLSIGIQPDILVCRSEKEIP
                     EELKAKIALFCNVEKQNVIQNEDTNSIYDIPLMLEKEGLAKLVCDHFNIKCTEPDLIE
                     WTDMVERSKNPKNSVKIALVGKYVELKDAYLSVAEALKHAGFDNSCDVDISWIHSDHV
                     NENNYKQILKDCDGILVPGGFGDRGIEGKILAAKYARENRKPYLGIGLGMQTAIIEFA
                     RSVLGYADADSFEFNAESGHKIIDVTDGQENIEKNGVAMRLGSYACKLVDGTKTKEAY
                     EGADMIHERHRHRYEFNNKFRDVCQENGLIISGISPDNKLVEIVELKDHPWFVGCQFH
                     PEFKSRPTRSHPLFKNLIKASIEFK"
     gene            9623..11170
                     /locus_tag="PLB33_03040"
     CDS             9623..11170
                     /locus_tag="PLB33_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOG62526.1"
                     /translation="MVKLFNDTFNINEIIDSNSTEVLLESEFGIADDMPDIEKLVTAE
                     GKAKLNNVTVTTDMIIVDGRLIYNIIYRSNNENYTIHSMSGEIPFREEIAAEGVTEDM
                     EALSNIFIDYIDADKLSERNFLVKAVLILDTDIYMKRPVTYVSNLESDGSIQAKAKNI
                     KYTDTIAEISEEININDAVELSKGSGEIDKIIKSEADVYITNIDELNDKMLIEGICKV
                     GFMFIEDDELHTIGYVSEEFPFTHYLELASSSENMLKDIKIAVNDISYSITENFDDEK
                     KLIEFALPFTVNASFYDSVEKNIIMDCYSTDYELELQSEKVKLSSLKNLTNEVVKYEN
                     SFEVLTGSIKDIYSVDVSPKVSEKRISDNKYIVDGFLDVNLLYLNGDINKIDKAFASL
                     PFTAGFPIDEDEALFEIHSDITTHKCSAYRKGNNSVVLNCDINVALKFKDDDEITVIR
                     DIAEKTPVDRSKMPSLIFRVVQSGETLWDIAKNYNLSINYLKELNDIPEGNVLTPGTK
                     IIIARMI"
     gene            complement(11241..12560)
                     /gene="dnaB"
                     /locus_tag="PLB33_03045"
     CDS             complement(11241..12560)
                     /gene="dnaB"
                     /locus_tag="PLB33_03045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOG62527.1"
                     /translation="MNLGKIPPHNAEAEQTVLASCMIDHTAVEKVVNLLSYDDFYYEA
                     NKEIYDSIKQIHMQNIPVDAVTVFEELKKRGKIDYIGGFEYLATLTENIITSKNVEAY
                     CNIIREKSTLRKLIGASQEIIEKSYKEDDEVQRIIELAEQRIFSISQNRSINTFSEIK
                     EVLMTVFNQLEERAKSGGGITGLATGYTDLDRMTSGLQKSDLILLAARPSMGKTALAL
                     NIAMNVVKNGASVALFSLEMSEEQYVQRIISQEAMVDSTKLRTGNLDDDDWTRLINTM
                     SLISNYKIYIDDTPSVTLFEMMSKCRRLKIEKGLDLIVVDYLQLMSDGGRTDNRQQEI
                     SNISRGLKALARELDCPVLALSQLSRAPELRNDHRPVMSDLRESGAIEQDADVVMMLY
                     RDEYYNKEESEKKGITDVIITKQRNGPVGTVELAWIGQYTKFGNIQK"
     gene            complement(12582..13028)
                     /gene="rplI"
                     /locus_tag="PLB33_03050"
     CDS             complement(12582..13028)
                     /gene="rplI"
                     /locus_tag="PLB33_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOG62528.1"
                     /translation="MKVILLEDVKNVGQKGQIINAKDGYARNFLFPKKLAIEATEANL
                     KNLENQKRKKEEQEKKILEEAKVLEEELLKKTIVIKTKAGENGKLFGSITTKEISDVL
                     EKEHKILVDKKKFDLEEPIKSVGEYYVKIKLHPTVTAKLKVIVTEK"
     gene            complement(13025..15028)
                     /locus_tag="PLB33_03055"
     CDS             complement(13025..15028)
                     /locus_tag="PLB33_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOG62529.1"
                     /translation="MDKKTPVFLKDDSFIYFVVIVLLIIIFLIESMFIYAASAGLVLV
                     IITAFTLRKQILRSRELKNYLINYTKNLENLSINSFYYLPLPICIISLNGKVFWYNNK
                     FKELFPENIDIIENVDEFVGGFPLNSMDGNKEGTISNIEVPETGRSFNVLYYELEEGR
                     FGEGKSYVCYWNENTAFVNLRNKYNDERPITMLIQIDNYEEISEKLELGEKSSMTAQT
                     EKILNSYAAEMNGFVLKYDNYKFLMMIENKYLETLEAKKFSMLDDVKSIKGTNDFNFT
                     LSIGTGAAAKNLTQLMEYSKGALDVALGRGGDQAVVKRVNSVKFYGGKSKAVEKRTKV
                     RARIISYALRQIIDQSSNVIIMGHRVGDMDSLGSAIGIYSIARSRGKKSYIVLNNVNH
                     ALKNMYGRMKKEESHYIESIITTEKALELADQNTLIVVLDTHRKSYTEAPELIDKIDK
                     VVLIDHHRRSEEYIENNLLDYIEPYASSTCELVAELIQYMEDHIKLTKFEADALMAGI
                     VVDTNSFTFKTGVRTFEAAAFLKRRGADNVEVKELFNESIEFMKKKTEIIERSEIVFD
                     DVAVSYIDEPTEDSNVAAAQCADELLTIKDVNMSFVLVRNKEYINISGRSVGDMNVQV
                     ILERLGGGGHLNMAGAQVQTDDMEEAKARLYEAIAQYKKEIKP"
     gene            complement(15043..16005)
                     /locus_tag="PLB33_03060"
     CDS             complement(15043..16005)
                     /locus_tag="PLB33_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOG62530.1"
                     /translation="MNRNLKTSNITESAMITGILVIIAYLSSFITLLMFFYPTPAIIL
                     GKRKGLKYSVLSLIASDLIISMLLGLQTGLVFLILYTPLALALTYGVCRNEEANKTIL
                     FGSAAYMISFVLYILLLNYIMGINFIERIAEIYEQSFETTRGLFNNIPDQLRTEQIEQ
                     YINDIEKMAPMMNYIVTNVFPAVLIVASVVTSYINYMVASKFAVRFSINVKQHEGIAY
                     FSFPRNFMISMAGLLLLSFLLRLLNINVGIIQMNLFIIVFMAMLLQGVAVLKFFVDKS
                     RMGKFARNLLLVFIVLMSINFSIAYAVIGLIDLTVNLRKINRAV"
     gene            complement(16110..17837)
                     /locus_tag="PLB33_03065"
     CDS             complement(16110..17837)
                     /locus_tag="PLB33_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG62531.1"
                     /translation="MNILMNIKHELQHIIEAMHAVTNVDITIVDESLQRIATTVNEEY
                     SLGAKAPIYSAFHKCIMTGEQYFLENPKENPICFDCENVQSCKELVELCIPIKFNGKI
                     LGVIGMCAYDEKARQNLLMNRESFMNLESQLVNIISTVISEKKIMKMLEYSSSELMTL
                     INSLNEGIIIINNNYEIINTNNYANEKLNINTSKIPDLWNLLSEKLYNNLISKNFIGE
                     VGPVKLNNTEFVINAYPIMVKDEREGTVLVLSDFNKMKESVIESNRKKDIVTFDDIVG
                     ESEALKQARREAIQVADSDASILILGETGTGKEVFARAIHNLSYRKNEVFMAINCGAI
                     PENLIESELFGYEKGSFTGASVTGNPGKFEICKDGTLFLDEIGELPLSMQVKLNRALE
                     EKEILRVGGNNPIKVNPRIISATHCSLASMVENNLFRDDLYYRLNVVPIHLPPLRERG
                     YDVIILSRYFLNRFNNVYKKQLQGFTPEAERLLMGYKFPGNIRELRNLIEYAVIFEEN
                     SYIGAENLRKKMGTSTSENLKLSELTRLYEKSVINSKIQELGGDLESKKKAAEHLGIS
                     IATLYRKLE"
     gene            18193..>18407
                     /locus_tag="PLB33_03070"
     CDS             18193..>18407
                     /locus_tag="PLB33_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HOG62532.1"
                     /translation="MKTNLLTNILKDQVTPALGCTEPGAVAYAVARAKEILGSEVNKL
                     NLEVDKNILKNGFSVGIPGTEEHGIIFA"
     assembly_gap    18408..18507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18508..19236
                     /locus_tag="PLB33_03075"
     CDS             <18508..19236
                     /locus_tag="PLB33_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035108936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG62533.1"
                     /translation="LIDYVNNVPIENISFIQNGIDMNLRIADAGLKETPGVGLSNYLY
                     NKAGDVFTMAKAYTAAASEARMSGYPLPVMSSAGSGNHGLVAVIPISYIGRENKISNE
                     RILRSVTLSHLITIFVKVYLGALSPVCGCGVAAGVGCAAGLTYMLGGDNEQIKGSINN
                     MIAGISGMLCDGAKLGCAYKLSISVDAAVDAAKMALEKIYIPEDNGILGCTAEKTIQN
                     LAQVSNIGMDHTDEVILDVIINKC"
     gene            19323..20690
                     /locus_tag="PLB33_03080"
     CDS             19323..20690
                     /locus_tag="PLB33_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG62534.1"
                     /translation="MKKRISLLIVVLMVILSFTSAWAIEIDEDKFDQDIEFMKKVIYF
                     VLDKYQYEVSQEDIINGLYDGFFSVLDDYSTYYTPKEYQALLEDTAGEFSGIGVQIMD
                     INGQVVVVTPLPGSPAIEAGIKAGDIIKTVDHYDITGVTSSQASLLIRGEEGTPVKIG
                     ILRGKENLTFDLIRRAIVINNVEGKILEDNIGYLKVTGFSDNTAELVEKELELFDEKN
                     VKKIIIDMRNNGGGTLQSAVDLLNLFVTEGPVVYVKYAAGKEDIYESKLKDKKYEIAV
                     LINEGSASATEIFAGAVKYKNEGIIIGTNSYGKGVVQSLYHLTDGSGVKFTTAEYFSV
                     DRIPVHKIGITPDIAVENEKTDISEYPKFSKEQKPELGNVSLDVLAAEMILMELGYEI
                     NQPDGVYDIQSFEQVRKFQEDNLLYPYGTIDFATQDALYNALIKHMESNLEDLQLKAA
                     IEALK"
     gene            complement(20756..21307)
                     /gene="yyaC"
                     /locus_tag="PLB33_03085"
     CDS             complement(20756..21307)
                     /gene="yyaC"
                     /locus_tag="PLB33_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOG62535.1"
                     /translation="MNPIEVHYKNSFAKIKIGNFLAEHMNNAAIVCIGTDKCIIDSLG
                     PLTGTLLMKENIKIDVYGTLEKPVHAMNINEYIKNLRSKNYDCIIAVDACLSNKKSQG
                     IIEVIEGSITPGKGIGKFLPEIGDISIIGIVNSSDKEFHDLVQDTRLSLIYEMAETIC
                     GGILNACSMNISESFDQASISSV"
     gene            complement(21359..22102)
                     /locus_tag="PLB33_03090"
     CDS             complement(21359..22102)
                     /locus_tag="PLB33_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOG62536.1"
                     /translation="MWERAELKARAKQVLKENYWKSFLISLVLLLSGADSRGGGSSGR
                     GAGERIGEWIDYSEYLIIIIAVVILVIAFRVLIGYALEIGSRKFFVQSAQYKNDKGCY
                     NFAFDTGNFKGIISTMFLRDIYNFLWTLLLIIPGIIKSYSYRMVPYILADNPNFGADN
                     AITLSRKMMDGNKFALFVLELSFLGWFLLGLLAFGVGILFVNPYVYATEAQLYLVLRD
                     TAIDQGYCTAEDLNLYNDFVKHDGYYWEE"
     gene            complement(22221..22802)
                     /locus_tag="PLB33_03095"
     CDS             complement(22221..22802)
                     /locus_tag="PLB33_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOG62537.1"
                     /translation="MPNVTAFLSYVIITTFTPGPNNIMAMSNASRYGFKKSIRFNAGV
                     FFGFFIIMVLSSFLSITLYNIIPSIKPLMTFIGASYILWLAWKIYKSKPHADDESKTT
                     SSFTAGMMLQFVNVKVIIYSITIVSTFIAPYYNSVFALSMFSLFLASVSFMSTCSWAM
                     FGAIFQKFLFKNERTVNIIMALLLVYTALSFYI"
     gene            complement(22898..23548)
                     /locus_tag="PLB33_03100"
     CDS             complement(22898..23548)
                     /locus_tag="PLB33_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012620673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG62538.1"
                     /translation="MYLKQKKLNLNKTDDEIKLLLLYEINNHILLDEQPSIFLNSIVN
                     TNIFKEYPFYMLYKLKETEQSPIYHPEGNAWNHTMLVVDEAAKLRNKSRDERVFMWAA
                     LLHDIGKAETTKNKKGKITAYNHETVGADLCVKFLQEFTTDDTFIQKVKGLVRWHMQI
                     LHVVKDMPFADIKSMKEETDIREVALLGFCDRMGRTNVDTIKEKNNIILFLEKCKL"
BASE COUNT         7303 a         4428 c         3692 g         8050 t
ORIGIN      
        1 gaataaaagg aagtaaaggt ataaaatatc ctgtaataaa ttaattgagt gccgtaatag
       61 agggttaaga agcttacgat tgaaaagaaa ataattttat ttgttttctc attaaaacct
      121 gttgaaagta aataaaataa caaccccaag ggtatagaaa acaaacacat atagacatat
      181 ccgatattaa atattttgct atatgtaaat gcttttaaaa ttgtttcata atacagaatt
      241 aataagggaa aaaatacaag ccttaaaata tttttgttgg tattcataat gcttctttct
      301 gtcatatcta taaaattaat agctgacctg aggaaagcca tgaggctttc ctcgggttac
      361 tgacaaaccc tatatgtcat cctgagcgta agcgtcattt tactacaaca tttgctaatt
      421 tttcatgttc tatgaacctt tgaatattag ctacaatcag ctcagccagt ctctttttgt
      481 tttcctttac aattcctgat gcatgaggag tgatgtacac attttccaaa tcccacaatt
      541 catcgtcttc aggtaaaggt tccacttcaa aaacatccaa tcctgcataa gagattgatt
      601 tgttttttaa tgcttctgtt aagtcttttt gatttattat gctgcccctt gctatattga
      661 ttattgaagc actttctttc ataaatttaa gagtatcctt attaatcata tgataggtat
      721 ccttgtttaa atcaactgtt acaattatgt aatcactgtt agacattaaa tattccaagc
      781 cctttttacc tgatagtatt tcatcaaaat atgccatggc agatacggga gttctcttgt
      841 aaccaattat tttaacgccg aatccctgaa gtctctttgc aatttctgtt gcaattgagc
      901 ctgttcctat tattcccacg gtttgaccgt gaaattctgt tcttttctga cgtggctccc
      961 acttgtgctc tctttgactc cttagaaatt tcaaagcatt tgtattatgc ataagaattt
     1021 tacatacaac atcctctgct attggaatgg aataaatatc ctttccgttg cagaaggtaa
     1081 tatttctttt tttcatatag tctagatcca atgtatcgta tcctgctctg aaggactgaa
     1141 gccaccttat attgggcatc ttatcaagct tttccggtac aaaatccatc ggctctccaa
     1201 ttagcgcttc cgcttcgggg cattcatcaa tttcggtata aaattcaata tcaggaaatt
     1261 tttcagatac aatgtctaaa tattttttgt gaataattat tttcatatat actcctttat
     1321 tatataattt ataatgtacg tgtctatagc ctgactatct taatacacaa acagagggca
     1381 tactgccccc ttattgataa caaaatatat ctctaaacta tacagtatgc tacaatttta
     1441 taatcatccg gaactttata atctttttcc ggtttcccga acttccattt tatgtcatac
     1501 aatgtacttt ctataattgc atcaaaataa agcattataa ctgcagtatc tattttttct
     1561 tcatattcgg ttacataaat gtcttttttc actgccaaaa caaccgttcc gttatcaact
     1621 ataaatctcc aaggctggcg atttttgtat gatggagcca gtataccgta aaagaatcct
     1681 tccaacagac catagttcac cagttcatca gggtcggctt tttctcccca ttccttagaa
     1741 aaaaccaagt ctcttatgcc gagtctttcg cttacattat cctcaacaag ttttacattt
     1801 gatgaagtat tgtattttga acccttctca tacaaaactt tattttgtgg gtcttcataa
     1861 ccaagtgcta taagagcaac caactttttg tctgaatcga tacctagctt tttctttatt
     1921 tcttcgccgt ctttaaaagt tatccagcat gaagcaattc ctaaactcca tgctttcagc
     1981 ataatatcct gtgctctgta gcctgcattt tctatgtaat gatccttttc ttcggataag
     2041 aatatcaaat aatgaggagc ttcaatcatt ctgttgttat atccggcgga atcttttagc
     2101 ttatcgaaaa cttcatctct gcttcttcta attactccaa cttcaatatc atcaaccagt
     2161 cttttgccct gctcataata ttgttttaat tccttaagta attttgaatc cacttcttct
     2221 tttttgtaaa gtcttaccga tttaatttca gaaataatct ctttatattt catattaagt
     2281 ttcctcctta tgggtatatt gtaattatac ccaacttttc tgagaactaa caaaaatatt
     2341 atactgttaa aatgctatta caattcctcc atcatcggca tatatggtac tcaaccctgc
     2401 agaactgaat attttaatat ctttgaaatt atattttaat tttatggcat ctcttaaatt
     2461 ctcagctttt tcaaggcaat taacatgagt tattcctaag actgtacttt caaaatcaat
     2521 attgtattcg gcaagcatat caacaagcct ggaaaaagct ttctttctac ctcttacctt
     2581 ttcttttaga actatccttc cttctccatc atcacccatg atgggaaaaa tattcattat
     2641 gtttgcaagt gttgctttaa agttgctgat tctaccattc ttggccagat tgtccaatga
     2701 ttccaatata aatagagtgt tcatcttaga tatatacttt tctgtttgtt caattatttc
     2761 atggggattt aagttttcct caatcaattg tttaacttta agagcaagca aagattcgcc
     2821 ggcggcagct gtctttgaat caaaaatatg aataaatgat tcaggaaact tttcttttat
     2881 tgtttccatc gccattacgg cgctgttgta agagccgcta agctggcttg atattgttac
     2941 gataaaatta ttggcttgaa ctttaatttt attcatgaaa tcttccggag gagcacaagc
     3001 tgtttttatt tgagtgcggc tttttttcat ttttcttata agttccaata tatccgtatt
     3061 ttcatctaca atttcttcct catctattat aattctgaaa ggaaccctgg ggatatcttt
     3121 tgtatcttca ttaaaatcta cacaactatc tactactaaa tttgcttcca taccgaacac
     3181 tcctctcttt ataattatta tgattataac aaaaacataa atattaaaca atttttattt
     3241 gtttaataag atcagttttt ttgcacgttt caattttttt atttgcgatt ctctctttac
     3301 tgcatcagct ttattgttaa attcctcata gtattttaat ataacaggag ttcttcctct
     3361 cgtgtacttt gctccttttt ttgcattatg agtttttatc cttttatcca aatcagttgt
     3421 atatccggtg tacaatgttt tgtctctaca ttccacaata tatatataat gcatatgtcc
     3481 tccaattaca caataatatc tattttaaca tatttttaca aaagttgaaa gcataatctt
     3541 tcaaaatacg cattgtaatc cttaataagc agcttgtaac ggtgttgact tttgtttttt
     3601 gttatattaa aattaagtaa taatgaggag gccaaaaatg agaataggta taatcggagc
     3661 catggatatt gaggtggaag agcttataaa ttccatggat gatataaaaa aggaaaccat
     3721 aagccgcata gatttttatg agggaaaact gcaggaaaaa gatgtggtgg tggcaaaatg
     3781 cggtgtaggc aaggtgcatg cagctatttg cactcaaact atgatattga agtatgctcc
     3841 ggatataatt atcaacacag gtgttgcagg cagtttaagc cctgacctca acattgcgga
     3901 tattgtaata tcagacggtg tggtacagca tgattttgat atttcaccct ttggtaaacc
     3961 ggtaggtttg attaccggaa ttgatttaat taaaattcca tgcaatgaag aattggtaaa
     4021 acaattagta aaagcggctg aagtcttaga ggatacaaat atagccgtgg gaactgtagc
     4081 ttcaggcgac cagtttatct gcagcaagga aaagaaggat tacatagtaa ataactttaa
     4141 tgccctttgt actgaaatgg aaggcgcatc aataggtcat gtatgcttta taaatgatgt
     4201 gaatttctgc atagtaagag ctatgtctga caaggctgac ggaactgcca acatggactt
     4261 tgacgttttt gtgaaaattg cagtagcaaa gtcgattaaa ctggttaata acttcttaaa
     4321 gcagctatag atggggggtc acaccaaaca aggaatgtcc cccttcgaaa ttacggaact
     4381 aatatgtcta tggcattatg tacggaagaa atttctgcag gtgcgtatcc ggcagggtct
     4441 ccatctgcat caattaataa ttccttttcg ggaattattt ttatattttc tgcctgataa
     4501 tagtgaactt ttggatgagt tatatgctgt ccgttaaaaa ccttggtgaa aatctgaaac
     4561 aactcggcat tacttgtttt ttcaaaaata actatgtcta acaagccgtc atctaattca
     4621 gccttaggac acaagtacct aaatccgcct gcgccggcag aattaagtac aaggaaaagc
     4681 aaagttttat agtcatattg ctttccgtca ataatataat taatattttc gcaaacctcc
     4741 aattcgtccg gaaccttaac cgctgcccga tagtaatagg catactttcc aagtattgtc
     4801 tttgcatcga ttgttacctt gtgtgaaata gtagtaaaag ctcctcctcc tactacattt
     4861 acaaaataac cttcatttac tctccccaca tcgattgttt taaatttcct ttgcataata
     4921 agagaggcaa aattttcttg agcttttgga aggttaagct gctcgccaaa gtcattgact
     4981 gttcctgccg gcattattgc caatttcggt ctgtagcagc atttcatcat cccattgaca
     5041 acttcattta ctgttccgtc accgccgcat gctataatca tctcatagcc gtattgacac
     5101 gctcttttgg cagcttcttc tccatctcct ttttttttgg ttgcataaaa taaaaactct
     5161 gcatcctcaa cgttcattag tgtttcggct gcaccaaata ctttatatgc tgcaagttct
     5221 tttcccgaag atggattgta tattacaaaa tatctattca tacttatcac ctgtatcttt
     5281 catgtttcac cgcctaatgg ggcaggatat ctttgtcttg gttatataat tatagcattt
     5341 tagttaaaaa tttcaacaat taatcttcta caaacttata ataaaggggc caatagtctg
     5401 catacagatt ccttaaatct tattaaaatg cccctctcca agtacaactc tcttgtcagc
     5461 tccattgaat aatttaaatc atccgtgaat atatcataca gcatttggga tgtctttgta
     5521 tcatatatta ttgcattgca ctcgaaatta agtttaaaac ttcttacgtc aaaatttgca
     5581 gttcccacag atgcaacagt gccgtcaatc accagggtct tggcatgtaa aaatccgtat
     5641 tcataaatat aaaccttaac tccatacttt aacagtcccc cgcaatacca gtaagtagcc
     5701 cagtaaacaa aaggatggtc aggtttgcaa ggtatcataa tccgaacatc cacccccgat
     5761 gcagcagcaa ttttgatagc ttcaaataca gaggcatcag gaataaaata aggagtttga
     5821 attaaaatac tttttttagc cgaatttatc attttcacat aattactttt tataacttca
     5881 tccgatttgt caggtccgct gcttattatt tgaattccca ccttgccttg aatcctgttg
     5941 tcgggaaaat atttcggcaa gaacattaac tcctgtttgg aagcatggct ccagtcaagg
     6001 aaaaaccttg tctgcaggtc tattaccgca tctccnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnncaatc tcattgtttt gtgtatagga aaagccataa taaaataaat
     6181 tcatttttat taaatcttta tagttttcta tattcttatc attaaaaaca agtccccctg
     6241 tactgaacaa ttctttttga accttaatat aatctccaaa tgtttttttg gcataaactt
     6301 tcataataaa gagctgtttt ctgctgaagt tttgtgccag caacaaatac aataaaaatc
     6361 cgaagccggg gaatatcaat agaaccaata cccatgcaag tgttgcggta gggtcttttc
     6421 tttccaaaaa tattactatc attgccatac aaatattaac tatataaatc aatgatatag
     6481 agttaaataa ttccacaata atcctcccga actacctttt tttattatta tatgaacatc
     6541 tcaattagta taaacattat cccaatgatt gaaaatgtaa aaagtttgtg gtagtatact
     6601 tattatcaac acaaaaggaa gaaattatga aaaaaccatc aaagtattgg attcttgcag
     6661 gtacattttt tacttctgtt tcttcaataa ttataagatt ctctgaagca cctgctctgg
     6721 ttatttcagc ttacagaatg ctctttactt gtatgatgct ctttttgcct gtctacataa
     6781 aaaacagaaa tgaatttaaa agtctgaata aaagcaactt catcatgtgt gttataagcg
     6841 gaatatttct tgcatttcac tttgcttcct ggattaagtc gattcaaatg actacaattg
     6901 caaattcaac cgttcttgta tcgtgcagcc ccatatttgt agcagcaatc aactattatc
     6961 tgtttaaaga aaaaataacc tataagatga tagttggcat ttctatgtcc ctgttaggta
     7021 caataataat aggaatgggt tcatctcaag gaaatgaaag cagcatgatg cttggaaata
     7081 tcctggcatt catgggtgca atatttgttg ctggttattt ggtaataggc ggatttgtaa
     7141 gaaggaccgt aagtgcaggg gtatatgttt ttattgttta ttttgtatcg gcaattgtat
     7201 tgtttattat gtgctttgta acgggaatac ccgtatatcc ataccccgcc aaagagtata
     7261 tgttattttt cctcttaggt tttttcagct caatacttgg ccatacagct tacaattatt
     7321 taatgaaata ttattcatct acgttaattt ccgtatcaac attaatggag ccgattttcg
     7381 ccagccttat ggcaatgttt atttttagtg aaataccatc cttatatacg gtggtcggcg
     7441 ggattataat aattggaggc atatattatt atctgatgac acaaaacaca annnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaaatata agacagtgag tcactgtctt
     7621 atttaaattc tatcgaagct tttattaaat tcttgaacaa aggatggctt cttgtaggtc
     7681 ttgatttgaa ttccggatgg aactgacaac ctacaaacca aggatggtcc tttagctcta
     7741 ctatttcaac caatttgttg tccggtgaaa ttcctgaaat tataagtccg ttctcctgac
     7801 acacgtccct gaatttattg ttaaactcat atctgtggcg atgcctttca tgtatcatgt
     7861 cagcaccttc atatgcttct tttgtcttag tgccgtcaac aagcttgcac gcgtagcttc
     7921 caagcctcat cgctacgcca tttttttcga tattttcctg cccatccgtt acatcaataa
     7981 ttttatgacc ggattcagca ttaaattcaa agctgtcggc atcagcatat cctaagactg
     8041 acctggcaaa ttcaataatt gctgtttgca tgccaagtcc tattcctaaa tagggctttc
     8101 tattttctct tgcatatttt gccgcaagaa ttttaccttc tattcccctg tcgccaaaac
     8161 cgccgggaac taatattccg tcgcaatcct taagtatttg tttatagttg ttttcattga
     8221 catgatctga gtgtatccaa gaaatatcaa catcgcaaga attatcaaat cctgcatgtt
     8281 tgagtgcttc cgcaactgac aaatatgcat ccttaagctc tacatacttg cccacaagtg
     8341 ctattttaac actatttttt ggatttttag agcgctcaac catatcagtc cactctatta
     8401 aatccggctc ggtacatttt atgttgaaat ggtcgcagac taattttgca agaccttcct
     8461 tttccaacat taaaggaata tcatatatgg aatttgtatc ttcattttga ataacatttt
     8521 gtttttcaac attacaaaat aatgcaattt ttgccttcag ttcttcaggt atttcttttt
     8581 cgcttctgca caccaatata tccggctgaa tacctatgct taaaagctct ttaacgctgt
     8641 gctgtgtcgg ttttgttttc agttctcccg ccattgacaa ataaggaagc aaggtaacat
     8701 gtatatacaa tacattttct cttcctacct cgtaacgaat ttgacgaata gcttccaaga
     8761 agggctggct ttcaatatct ccaacagttc caccgatttc ggttataact atatcaacat
     8821 cctcaactga ggcaattctt tttaacatac atttgatttc attggttaca tgaggaataa
     8881 cctgcactgt tcctccatgg taatcccctc ttctttcctt attcaataca gcccagtata
     8941 ttttccccga tgtgatgctg ctgtattgag ttaaatctat atcaactatt ctttcatagt
     9001 gtcctaaatc taaatctgtt tcggctccgt cgtcagttac atacacttct ccatgctgat
     9061 aagggctcat cattcccggg tccacgttta aataagggtc aaatttctgc aatgttactt
     9121 taagtcctct gctttttaaa agccttccca atgaagctgc tgttattcct tttcccaatg
     9181 aagaaacaac tcccccggtt acaaaaatat atttcggcat attttcctcc tgataactat
     9241 tattccaata attttaatta atataagatt ccctgcccct atttatttaa ggtatgggga
     9301 cacacctccc atctgatcaa ccggcaggag gaatgtcccc aattgcttca taccttgtta
     9361 acataaaaaa gagacaaaaa ggggtttttt ttttagtctc tattaattcg aatctctttg
     9421 caattattta attaatttgg caatttaaag aattgtcatt aaattactaa atcattttat
     9481 attaaataaa taaaaaggtc aatccttttt aatacttttt gaaaaaattt tatttataag
     9541 ttgtactatt gaaatttaaa cggcatatac atttataaaa gaataatgcg taattccgta
     9601 ttaacttaaa ggaggattta ttttggttaa attatttaat gatacattta atattaatga
     9661 aattattgat tcaaattcaa ctgaagtatt gttggaaagc gagtttggaa ttgctgatga
     9721 catgccggat attgaaaagc tcgtaaccgc ggaaggaaaa gcaaagctta acaatgttac
     9781 ggtaacaaca gatatgataa tagttgatgg caggctgata tataatatta tttatcgttc
     9841 aaacaatgag aattatacaa tacattccat gtcaggcgaa atacctttca gagaagaaat
     9901 tgccgcagaa ggcgtaactg aagacatgga agcattatca aatattttta tagattatat
     9961 tgatgccgat aagctgtcag aaaggaattt tcttgttaaa gcagtattaa tactggatac
    10021 tgatatctac atgaaaagac ctgttaccta cgtttcaaat ttagaatctg acggaagcat
    10081 ccaggctaag gctaaaaata ttaaatatac ggatacaata gcagaaatat ctgaagaaat
    10141 aaacataaat gatgcggtag aacttagcaa gggttcaggc gaaatagata aaattattaa
    10201 atcagaagct gatgtttaca taacaaatat agatgaatta aatgataaaa tgctcataga
    10261 gggaatatgc aaggttggat ttatgtttat tgaagacgat gagcttcata ctatcggata
    10321 tgtatctgaa gaattcccct ttactcatta cctagagctt gcttcttcaa gtgaaaatat
    10381 gttaaaggac ataaaaattg cagtaaatga tatatcttat tcaataactg aaaattttga
    10441 tgatgagaaa aaacttatag aattcgcact gccatttacg gtaaatgcat cattctatga
    10501 ttctgttgaa aaaaacatta tcatggactg ctattcgact gattatgagt tggagctgca
    10561 atcagaaaaa gtaaaattgt catcgcttaa gaatttaact aatgaagttg taaaatatga
    10621 aaattctttt gaagtgctta caggctcaat aaaggatatc tattcggtag atgtaagccc
    10681 gaaggtttct gaaaagagaa tatctgacaa caaatacatt gtagatggat ttttggatgt
    10741 aaatttgctt tatttaaacg gagacataaa taaaatagac aaagcatttg cctcacttcc
    10801 gtttaccgcc ggcttcccga tagatgaaga tgaagcatta tttgaaatac attctgatat
    10861 tacaactcat aaatgcagtg cctacagaaa aggaaacaac tcggtagttt taaactgcga
    10921 tattaatgta gcattaaaat tcaaagacga tgacgaaatt accgtaatca gggatattgc
    10981 cgaaaaaacc cctgtagaca ggagtaaaat gccaagcctg attttcagag tggttcagtc
    11041 cggagaaact ctgtgggata ttgcaaagaa ttataattta tcaataaact acctgaaaga
    11101 gcttaatgat attcccgaag gcaatgtact tacaccggga acaaaaataa taattgcaag
    11161 aatgatatag atatgaggtt catcgccgac gctcaggaat acagtatgtt atcctgagcg
    11221 cagcgatgaa tctttttttg ctacttttgt atatttccaa actttgtata ctgacctatc
    11281 catgccagct caacggttcc tacgggaccg tttctttgtt tggttatgat tacatccgtt
    11341 ataccttttt tctcggattc ttccttgttg taatattcat ctctgtaaag catcatcact
    11401 acatcggcat cctgctcaat agccccggat tctctcaagt cagacataac aggtctgtgg
    11461 tcatttctaa gctccggtgc acgggaaagc tgagacaggg ccaataccgg gcaatcaagc
    11521 tccctggcaa gagccttcag tcctcgcgat atattggaaa tctcctgctg cctgttgtcc
    11581 gtccgtcctc cgtcggacat gagctgcaag tagtcaacta caattaaatc taaacctttt
    11641 tcgattttaa gcctcctgca tttagacatc atttcgaata atgtaacgga tggcgtgtca
    11701 tcaatatata ttttgtaatt tgaaatcaag ctcatggtat taatgagcct tgtccaatca
    11761 tcgtcgtcta aattgcctgt cctgagtttt gtgctgtcaa ccatggcttc ttggcttata
    11821 attctttgaa cgtattgctc ctctgacatt tcaagactga aaagagcaac agaagcgccg
    11881 ttcttaacaa cattcatggc aatattcaag gcaagggcgg tttttcccat tgatggtctt
    11941 gctgcaagca gtatcaaatc agatttctga agtcccgaag tcattctgtc taagtcagtg
    12001 tagcctgttg caagacctgt aatgccgccg ccgctcttgg ctctttcttc aagctggtta
    12061 aatactgtca taagaacttc tttaatttca gaaaatgtat tgatgcttct gttttgtgaa
    12121 atggaaaaaa ttctttgctc agccaattct atgattcttt gaacttcatc atcttcctta
    12181 tagctcttct ctataatttc ttgagaagcg ccaataagtt tacgcaaggt tgatttttct
    12241 cttattatat tgcagtaagc ttcaacattt tttgaagtta ttatattttc ggttagtgtc
    12301 gctaaatatt caaatccgcc tatatagtct attttgcctc tttttttcag ctcctcaaaa
    12361 acagtgaccg catcaacagg tatattttgc atgtggattt gtttaatgct gtcatatatt
    12421 tctttgtttg cttcataata aaaatcgtca taactcaaca aattaactac tttttcgaca
    12481 gctgtgtggt caatcataca ggaagctaag acagtctgct cagcttccgc attatgagga
    12541 ggaatttttc ccaaattaat tatatcagac atttttaccc cttacttttc tgttactatt
    12601 acttttaatt tagcagttac cgtcggatga agttttattt tcacatagta ttcaccaaca
    12661 gatttaatag gctcttctaa gtcaaatttc tttttatcga ctaaaatttt atgctccttc
    12721 tctaaaacat ccgatatttc ttttgttgta atagagccga aaagctttcc gttttcaccg
    12781 gcctttgttt ttataacaat ggttttctta agcagttctt cttcaagaac tttggcttct
    12841 tccaagattt tcttttcctg ttcttctttt ttcctttttt gattttctaa gttttttaaa
    12901 ttagcttcgg tagcttctat ggctaatttt ttgggaaaaa ggaaattgcg ggcatatccg
    12961 tccttagcat taattatttg acccttttga ccgacatttt taacatcttc caatagtatt
    13021 actttcatgg tttaatctcc tttttatact gagctatggc ttcatacagt ctggcttttg
    13081 cttcttccat gtcatcagtt tgtacttgag ctcctgccat gttcaaatgg cctcctcctc
    13141 caaggcgttc caaaattacc tgcacattca tatcccctac ggagcggccg cttatattta
    13201 tatattcttt gtttcttaca agcacaaaac tcatatttac atccttaatc gtaagcagct
    13261 catcagcaca ttgggctgct gccacgttgg aatcttcagt gggctcatca atatatgata
    13321 ctgctacatc atcaaataca atttcacttc tttcaataat ttctgtcttt ttcttcataa
    13381 attcgatgct ttcattgaac aattccttaa cttcaacatt gtcggcacct cttctcttca
    13441 aaaaggctgc tgcttcaaaa gttcttacgc ctgttttgaa tgtaaatgaa tttgtatcca
    13501 ccactattcc tgccataaga gcatcggctt caaattttgt aagcttaatg tggtcttcca
    13561 tgtattgaat caattcagcc acaagctcac atgttgatga agcatagggt tcaatgtaat
    13621 ccaataaatt gttttcaata tattcttcac ttctcctatg atggtctatc aatacaacct
    13681 tatcaatttt atcaataagc tcgggagctt cggtataact tttcctgtgc gtatccaaaa
    13741 caacgatgag cgtattttgg tcggcaagtt ccaatgcctt ttcagttgtt atgatggatt
    13801 caatataatg gctttcttct tttttcattc ttccatacat atttttcaaa gcatgattca
    13861 cattattcag cactatgtat gatttctttc ccctgcttct ggctatgctg taaattccta
    13921 tggccgagcc gaggctatcc atatctccca ctctgtgccc cataataatc acattagagc
    13981 tttggtcgat tatttgcctc aatgcataag atataatcct ggccctgact tttgtccttt
    14041 tttcaacggc cttgcttttg ccgccgtaaa atttaacgga attcaccctt ttaacaactg
    14101 cttggtcgcc tcctcttccc aaagctacat caagcgctcc tttggaatac tccataagct
    14161 gagttaaatt cttagccgct gcaccggtac ctatgctgag cgtgaagtta aaatcattgg
    14221 taccctttat tgatttaaca tcatcaagca tggaaaattt ctttgcttcc aatgtttcaa
    14281 gatatttgtt ttcaatcatc atcaaaaatt tataattgtc atatttcagc acaaacccat
    14341 tcatttcagc agcataagag ttaagaattt tttctgtctg tgcagtcatg gaagattttt
    14401 cacccagctc taatttttcc gatatttctt cataattgtc tatctgaatc agcatagtaa
    14461 taggccgttc atcattatat ttattcctca agttgacaaa tgccgtattc tcattccaat
    14521 agcaaacata ggattttcct tctccgaatc gcccttcttc aagttcataa tacaatacat
    14581 tgaaacttct gcccgtttca ggaacctcta tattgcttat agtgccttcc ttgtttccat
    14641 ccattgagtt taacgggaat cctccgacaa attcatctac attttcaatt atatctatat
    14701 tttcaggaaa cagctcttta aacttattgt tataccagaa tactttgccg tttaaactga
    14761 ttatgcatat gggcaagggt aaataataaa aagaatttat tgataagttt tctaaattct
    14821 tcgtgtaatt aatcagataa ttcttaagtt ccctactgcg cagaatttgt tttcttaatg
    14881 tgaaagctgt tattataact aagactaaac cagctgaggc agcataaatg aacatacttt
    14941 caattagaaa aattattatc aataatacta taactacaaa atatataaaa ctgtcatctt
    15001 tcaagaatac cggagttttc ttatccatat tgcctccttc aactatacgg ctctgttaat
    15061 ttttcttaaa tttacggtta aatcaatcaa tccgattact gcataagcaa tgctgaaatt
    15121 tatgctcata agtacaatga aaaccaataa taaatttctt gcaaatttac ccattctact
    15181 tttatctaca aaaaatttta aaactgcaac cccttgtaaa agcatagcca taaagactat
    15241 gataaataga ttcatctgta taattccaac gttaatattc aataaccgca ataaaaacga
    15301 taatagcagc aatccggcca tagaaatcat aaaatttctt ggaaacgaaa aataagctat
    15361 tccttcatgt tgttttacat ttattgaaaa ccgtactgca aatttacttg caaccatata
    15421 attaatatat gatgtgacta ctgatgctac tatcaatacc gcaggaaata catttgttac
    15481 aatgtaattc atcatcggtg ccattttttc aatatcattt atatactgct caatttgctc
    15541 agttctaagt tggtcaggaa tgttgttaaa taatcctctt gtcgtttcaa agctttgctc
    15601 atatatttcc gcaatccttt cgataaaatt aatgcccatt atataattca aaagtaatat
    15661 gtaaagaaca aatgaaatca tataagcagc cgaaccgaac aaaattgttt tattagcttc
    15721 ttcatttctg cagacaccat atgtaagtgc aagagctaaa ggtgtatata aaattaaaaa
    15781 tacaagtccc gtttgtaggc ccaatagcat ggaaattatt aaatccgaag ctattaatga
    15841 taaaaccgaa tatttaagcc ccttcctttt acccaaaata attgcagggg tgggatagaa
    15901 aaacattaat aatgtaataa aagatgacaa ataagcaatt ataaccaata tcccggttat
    15961 cattgccgat tccgtaatgt ttgatgtttt taagtttctg ttcatcttta cctcacatat
    16021 ctatatacga gagagatatc attgtgttgt tttaacattt taacctataa tctaatcaaa
    16081 gtcaaaaact tatttcaagc tttacgtaat tactccaact ttctgtacaa ggtggcaata
    16141 cttataccaa gatgctcggc agcctttttc tttgattcta aatcaccgcc taattcttga
    16201 attttactgt ttatgacaga tttttcgtac agccttgtca attccgacaa ttttaagttt
    16261 tcggaagtgc tggttcccat tttttttctg agattttctg ctcctatata gctgttttcc
    16321 tcaaatatta ccgcatattc aattaaattt ctaagctctc ttatgtttcc gggaaacttg
    16381 tatcccatca ataaccgctc tgcttcaggc gtaaaccctt gcagctgctt tttatagaca
    16441 ttgttaaagc ggttaagaaa ataccttgat aaaataatca cgtcatatcc tctttctctc
    16501 aaaggaggaa gatgaatggg aactacatta agtctgtagt ataaatcatc tctgaataga
    16561 ttattttcaa ccatagatgc caaggagcag tgagtagcag aaattatgcg gggattaacc
    16621 tttattggat tattacctcc cactcttaat atttcttttt cttcaagggc cctgtttaat
    16681 tttacttgca ttgataaagg aagctcacct atttcatcca aaaacaatgt accatccttg
    16741 caaatttcaa atttgcccgg attgccggtt acactggcac ctgtaaatga gcccttttcg
    16801 tatccgaaaa gctcgctttc aattaaattt tcaggaattg ctccacagtt tatagccatg
    16861 aacacttcat tttttctata gcttaaatta tgtattgctc gagcaaaaac ttcctttcct
    16921 gtacctgttt caccgagtat taaaatagaa gcatcgctgt cggccacttg tattgcttct
    16981 ctccttgcct gtttcaaggc ctcgctttcg cctacaatat catcaaatgt aactatgtct
    17041 ttcttcctgt tggattcaat taccgattcc ttcattttgt taaagtcaga caataccaat
    17101 actgttcctt ccctttcatc ctttaccatg atggggtagg catttattac gaattcagtg
    17161 ttattaagct tgacaggacc cacttctcca ataaaattct tggaaattaa attattataa
    17221 agtttttctg atagcaggtt ccacaaatcg ggaatttttg aggtgttaat atttaatttt
    17281 tcgttagcat aattgttggt atttataatt tcataattat tatttataat aataattcct
    17341 tcatttaatg aattaatcag cgtcatcaat tcagatgatg aatattccag catcttcata
    17401 atcttctttt cactgataac ggtagatatt atattaacta attggctttc taaattcatg
    17461 aagctttcac gattcatcaa caaattttgt cttgctttct catcataagc acacatgccg
    17521 attacaccta atatttttcc gttaaactta atgggaatgc atagctcgac taattccttg
    17581 cagctttgaa cattttcaca atcaaaacat atggggtttt ccttggggtt ttccagaaaa
    17641 tattgctcac cggtcataat gcatttgtgg aaggcagaat aaataggagc ctttgctcca
    17701 aggctgtatt cctcattaac agtggttgca atcctctgaa gagattcatc aactatggtg
    17761 atatctacat ttgttactgc atgcattgct tcaataatat gctgaagttc atgcttaata
    17821 ttcattaaaa tattcattta acctctaccc cgctcgaagg atatcttctt cattataata
    17881 tattataata caagatgctt attaatacta tactttaatt tgaaattttc aaaatattta
    17941 tcatttttga taattcttgc gcaaaactga taaaacgttt gtaagattca aattatgcaa
    18001 acttataaat tcctttataa tttatttatc atttttgata attttcttag atcattcttt
    18061 gactaaaggt ctatttttca atcaattctt aaaattaggc attggcatga ttcatgcata
    18121 taaattatta aagacgagaa actcacttgt acttgcagta tcaggttaaa taaaataaaa
    18181 gaggagatgc atatgaagac taatctattg acaaatatat tgaaagatca ggttactccc
    18241 gcattaggat gcactgagcc cggagcagtt gcttatgcgg ttgcaagagc aaaagaaata
    18301 ttaggctctg aagttaataa gcttaatcta gaggttgata aaaacatatt aaaaaacgga
    18361 ttcagcgtag gtatcccggg gacagaggag cacggtataa tatttgcnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnctt attgattatg taaataatgt tcccatagaa
    18541 aatatttctt ttattcaaaa cggaatcgat atgaatttaa gaattgccga tgcaggatta
    18601 aaagaaactc ccggtgtcgg tttgagcaat tatctgtata ataaagcagg ggatgttttt
    18661 acaatggcta aggcatatac tgctgccgct tcggaagcaa gaatgtccgg atacccttta
    18721 cccgtaatga gcagtgccgg ttcaggcaat cacggtttgg tggcggtaat tcccatatcc
    18781 tatataggtc gggaaaataa aatttcaaac gaaagaattt tgcgctctgt aaccttaagt
    18841 catttgatta caatttttgt aaaagtatat ttaggagcac tttcgccggt ttgcggctgc
    18901 ggagttgcag caggagttgg ttgtgcagca ggtctcacat acatgttagg aggagacaat
    18961 gagcagatta aaggctcaat aaacaatatg atagcgggaa tttccggcat gctctgtgac
    19021 ggggcaaagt taggctgcgc ctacaaactg tcaatatcag ttgatgccgc tgttgatgct
    19081 gcaaaaatgg ccttggaaaa aatatatatt ccggaagata acggtatctt aggatgtaca
    19141 gcagaaaaaa caattcaaaa tttagcacag gtttcaaata taggcatgga ccatacagac
    19201 gaagttatac ttgatgttat aattaataag tgctaacata ggtatgtcca ctcatagtta
    19261 agaaacatat ccgttcatgg tgtgtccgcc ctatgataat tctaaagaaa acgaggtgct
    19321 ttatgaaaaa gagaatcagc ttgttaattg tagttctaat ggtaatttta tcatttactt
    19381 ccgcatgggc aatagaaatc gatgaagata aatttgacca agatattgaa ttcatgaaaa
    19441 aagtaatata ttttgtgctt gacaaatatc agtacgaagt aagccaggaa gatatcataa
    19501 atggtcttta tgacgggttt ttcagtgttt tggatgacta cagcacttac tatacaccca
    19561 aggaatacca ggctctgctt gaagacacag caggagaatt ttcaggcata ggcgttcaaa
    19621 taatggatat aaacggacaa gttgtggtag taactcctct gcccggctca cctgcaattg
    19681 aagcaggaat taaagcaggt gatataataa agactgttga ccattatgac attaccggag
    19741 ttacttcaag tcaggcatct cttcttataa ggggagaaga aggtactcct gttaaaattg
    19801 gaattttacg cggaaaggaa aatctgacct ttgatttaat aaggagggct atagtaataa
    19861 ataatgttga aggaaaaata ttggaagata atataggata tttaaaagtt accggttttt
    19921 ccgacaacac agcggaactt gtagaaaaag aattagaatt atttgatgaa aaaaatgtaa
    19981 aaaaaattat aatagatatg cgtaacaatg gcggcggaac attgcagtct gcggttgact
    20041 tgttgaattt atttgttacg gaaggacctg tggtatatgt aaaatatgca gccggcaagg
    20101 aagatattta tgaaagcaaa cttaaagata aaaaatatga aattgccgta ttgataaacg
    20161 aaggaagtgc cagcgcaaca gaaatatttg caggggcagt aaagtataaa aatgaaggaa
    20221 ttattatagg aacaaactct tacggaaaag gagttgttca gtctctgtat catttaaccg
    20281 atggaagcgg agtaaaattt accacagcgg aatatttttc agtagataga atacccgtac
    20341 ataaaatagg aataacaccg gatattgcag tagaaaacga aaaaaccgat atttctgaat
    20401 atccgaagtt cagcaaggaa caaaaacccg agttgggaaa tgtcagcctt gatgtattag
    20461 ctgctgaaat gatacttatg gaattaggtt atgaaataaa ccaaccggat ggagtttacg
    20521 acatacaatc atttgaacaa gttagaaaat tccaggaaga caatttatta tacccatacg
    20581 gaacaattga ttttgctact caggatgcat tgtacaatgc attgataaaa cacatggaaa
    20641 gcaatttaga agatttacag ctgaaggctg ccatcgaagc attaaaataa caaaaactgg
    20701 ggacattcct cttacttcaa agactttccc taaagaagag gtgtgtcccc ataccttata
    20761 cactgcttat cgaagcctgg tcaaaacttt ctgatatatt catgctgcaa gcatttaata
    20821 ttcctccgca gatcgtttct gccatctcat aaataagaga aagtcttgta tcctgaacca
    20881 aatcgtgaaa ctccttatcg ctggaattta caatccctat tatagatata tccccgattt
    20941 caggaaggaa tttccctata ccctttcccg gtgtgattga gccctcaata acttctataa
    21001 ttccttgact tttcttattg cttaagcagg catccaccgc tattatgcag tcataattct
    21061 tgcttcttaa gttttttata tactcgttaa tattcattgc gtgaacaggt ttttccaaag
    21121 ttccgtaaac atcaattttt atattttctt tcatcagtaa agtacctgtt aagggtccta
    21181 agctgtctat tatgcattta tccgttccta tacatactat tgctgcatta ttcatatgct
    21241 ctgccaaaaa atttcctatt ttaatttttg caaagctgtt cttataatgt acttcaattg
    21301 gattcaaagg aaacaccccc tatgtgttag tatatacaca cagggggtgt tttatgcttt
    21361 attcttccca ataataacca tcatgtttta caaaatcatt ataaagattt aaatcttcgg
    21421 cagtgcagta tccttggtct atggctgtat ccctgagtac cagataaagc tgagcctcag
    21481 ttgcatagac ataaggattt acaaatagaa ttccaactcc aaaagctaac aaaccgagta
    21541 agaaccaacc aagaaaagac aattccaaga caaataatgc aaatttattt ccgtccatca
    21601 ttttcctgct taaggttatg gcattatctg caccaaaatt aggattgtca gccaaaatgt
    21661 agggaaccat tcgatatgaa taagatttta ttattcccgg tattatcaat aacaatgtcc
    21721 aaagaaagtt atatatatcc cttaaaaaca ttgttgaaat tataccttta aaattccctg
    21781 tatcaaaagc gaaattatag cagcccttat cgttcttata ttgagccgac tgtacaaaga
    21841 attttcttga gcctatttcc aatgcataac ctattaacac tctgaaagca attacaagaa
    21901 ttacaacggc aataataatt atcaaatatt cagaataatc aatccattcg ccgattcttt
    21961 cccctgcacc tctgccggat gaccctccgc cgcggctatc tgcacctgat aatagtaaaa
    22021 ctaaactaat taaaaatgac ttccaataat tttcctttaa tacttgtttt gctctagcct
    22081 taagctctgc cctttcccac atattccctc cttgttaatt ttttaaatta attatattaa
    22141 atttctgaca aggggacggg ggtattgtca tatacacaaa ttccctagca aagatgacag
    22201 cacccccgtc cccttgtcac ctatatataa aatgacaagg cggtatatac caacaacagt
    22261 gccattataa tattgacagt tctttcgttt ttaaataaga atttctgaaa tattgctcca
    22321 aacattgccc agctgcaggt ggacataaaa ctgaccgatg ccaaaaataa tgaaaacata
    22381 cttagggcaa aaacagaatt ataatatggg gctataaatg tagatactat ggtaatgctg
    22441 tatataataa cctttacgtt aacaaactgc agcatcattc cggcggtaaa agagcttgta
    22501 gtcttacttt catcgtcggc atggggctta cttttatata ttttccaagc aagccataat
    22561 atatatgaag cacctataaa agtcataaga ggctttattg atggtattat attatataat
    22621 gttatgctca aaaaactgct taataccata ataatgaaaa agccgaaaaa cactccggca
    22681 ttaaacctaa tactcttttt aaatccgtat ctgcttgcat tagacatagc cattatatta
    22741 ttaggtcccg gtgtaaaagt tgttataata acataagaca agaaagccgt gacatttggc
    22801 atagtgcacc tcatatattg ttatgaaata ataatacagc ttttacctga ttaagtcaat
    22861 aaatttacga aattctgcct atgaagctgc ttaatgatta taatttacac ttttccaaaa
    22921 acagaataat attgtttttt tcttttattg tatctacatt tgttcttccc atcctgtcac
    22981 aaaaacctaa taatgcaact tctcttatat ccgtttcttc cttcatagac ttaatatcag
    23041 caaaaggcat atcctttacc acatgaagta tctgcatatg ccatctgaca agtcctttga
    23101 ccttttgaat aaatgtatcg tccgtagtaa attcctgaag aaatttaacg cataaatcag
    23161 ctcccaccgt ttcatgattg taagccgtga tttttccctt tttatttttg gttgtttcag
    23221 cctttcctat atcgtgtaac agcgctgccc acataaatac tctttcatct ctgctcttat
    23281 ttctcagctt agctgcttcg tcaactacca gcattgtgtg attccaagca ttgccctcag
    23341 gatggtaaat tggtgattgt tcagtttctt taagcttgta aagcatatag aagggatatt
    23401 ctttaaatat attagtgttt actattgagt ttagaaaaat tgacggctgt tcatccagca
    23461 aaatatgatt atttatttca tataacaaca ataatttaat ttcgtcatca gtcttgttta
    23521 aatttaactt cttttgttta agatacataa ttacctctgt ctcataatta taatgtaata
    23581 tacatatcta acattccatc ttaaacaaaa aaatattctg ttaattaatt catataagaa
    23641 tatattatct ttttatgatt tgataaatat gatataatga atacaatcaa tgaagcaaag
    23701 gcctgtgcct ttgcaccaat gtattcattg tatcggtgtg aagcaagccg attttgcggc
    23761 ttgcactgat gat
//
LOCUS       DAOSHP010000017        23691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1695_length_23691_cov_9.695972, whole genome shotgun sequence.
ACCESSION   DAOSHP010000017 DAOSHP010000000
VERSION     DAOSHP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23691
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1695_length_23691_cov_9.695972"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    21..250
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            381..1367
                     /locus_tag="PLB33_03105"
     CDS             381..1367
                     /locus_tag="PLB33_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG62539.1"
                     /translation="MKNSTIKFIAILFLVSLIITGCSANTEANKPAGGKFTIGIAQLV
                     QHPALDASRQGFIDEMEKLGVEVEFIDQNAQADINNAQMIAEKFVKDDVDLIFSIATL
                     TSQAAKKAIEGTKIPLVFTAVTDPVFSELVTALDVTDNNITGVVDAAPIKENLELFKL
                     LKEDIKTVGIIYNIGESNSEVQVEQTKEIAAELGLTIETVGITSINDIPQAVDTISKK
                     VEGLYIITDNMVASAMELVAKLAGEKDLLTVSADGSHVDQGILISKGISYYVIGQQSA
                     RIAKQILVDKVDVADITVQASTEFEKKVNLKTAESLGLAKEHKAFEGAEFVE"
     gene            1364..2242
                     /locus_tag="PLB33_03110"
     CDS             1364..2242
                     /locus_tag="PLB33_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62540.1"
                     /translation="MNTLILTSIEQGLIFSVLAIGVIITIKILDFADMSVEGTFPMGA
                     FIFAKFITTGFSPIISTLLTFILGTLAGLLTYTLNIKLRIRALLSGILTATILYSANL
                     RLNGRSNIGLTSYDSIFDGFRPVIVLILIVLAVKILMDIFLRTEVGYLLIATGDNETL
                     VKSLGENSNKYKLIGLMLSNGLVSLSGALMAQYQGFSEISMGTGIIVVAIASIIVGDT
                     VLRNSKILKMTTRAIMGALIYKFIGALAIDLGLAPTDLKAINAAIVIIFLSYNTFSPV
                     KMRKARKIKAVEGVKC"
     gene            2236..3012
                     /locus_tag="PLB33_03115"
     CDS             2236..3012
                     /locus_tag="PLB33_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG62541.1"
                     /translation="MLDIINLSKTFNQGTDYEIRIFSNFNIHINKGECTAILGANGCG
                     KSTLFNIISGFLMQEKGIIKLNGKNINGLKENERAKFIGRVHQNPSAGVSPSLTILEN
                     ISLSDKKCQKFTLRKLIRKNKIEAYKEILRTLDLGLETKLDTEVKYLSGGQRQSLSLL
                     MATMNKPQLLLLDEHTAALDPKTSRLVMEKTQELIKTQNITTMMISHNARDALMYSDR
                     IIMLDKGEIILDLRKGQITEKELLNIYNSRNYGSPLTEAV"
     gene            3138..>3817
                     /locus_tag="PLB33_03120"
     CDS             3138..>3817
                     /locus_tag="PLB33_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG62542.1"
                     /translation="MEDVIKEIKNNPLFFNMSEDEIKFVLKCSNSTVKDLRDNYALFE
                     KDQKVKKLGIVIDGILNLESRKYNGNRVIVTTLEKNDLFGEALIYASEGSSPYDLVSS
                     GNSKVLLIPYKIFSNMCFNNCSFHAQLIKNMLTILSDKIVMLNNKMNILNAETLKGRI
                     AVYLLSLQKKSNSMIFNMPMNRQELAEFLNVKRPSLSRELSNMQKEKIIEVYRSSVKI
                     LDMEKLCE"
     assembly_gap    3818..3917
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3981..5075
                     /locus_tag="PLB33_03125"
     CDS             3981..5075
                     /locus_tag="PLB33_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG62543.1"
                     /translation="MKLKFIHTSDLHLGKKFDIKNFSLKERKKRRQELWDSFDEIIRT
                     AKIEEVQYLFIAGDLTDGEYISYKSLNKISSKFSSIPDTNVIITCGQSDPYNVNNMYE
                     YIEWPDNVYIIKNTETVEKLNFPENNLCIYSISWDNHAENKRTQPIYDISVDENKLNI
                     LMLHCDIDSSDRLPVNIDIIKNKFDYCPLGGRHNFRQITDNVVYCGTPEPLSFDEEGE
                     HGIIRGTLEKNNTQYSFLPIAKRKFINRQIELNISYSFNKILDLIKFSGDTFSNIKDY
                     VRIELTGEVNADISMDEIENEAKQFFYYIEFEDNYIYDTENRNYEGNEFNIIESYKLQ
                     FDKNDDNDDKLQQQAFKLGLEVLRKEKVVK"
     gene            5077..7752
                     /locus_tag="PLB33_03130"
     CDS             5077..7752
                     /locus_tag="PLB33_03130"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG62544.1"
                     /translation="MYINRLNLRAFGKFINRKIHFSNKFNIIFGENETGKSTIHNFIE
                     LVMYGFDEDTSRYNKYKPWNSPVYKGSIEIADEEKKHIISRDFLLDTIQVFKKTNETK
                     ENEYSVEGIGVPGEHFFNINRVSFNNTVSISQLGNKTEKELADELKNKITNLSNTKDE
                     DISIDRIMQSLYRIKEEAGSENNDKTLLGQYSLRLEELRNAKESSMNSNRQVMFLAME
                     KKKLQSKIHELNMKIEEKQKEISDYELFLEKQKFLRAQPVKKEIDNINDELKLIEDDV
                     TNVSAEDYKEASETESSLASMKNDKQRLIEEKEECISELELIESDLSNYIPEEFDIDK
                     LNIDYDIYKANNEKIDTLKMKMKTGRESINSINIDEINNFINDYREVEELNHKIEINN
                     VLTDSKNYELMKKFGKKQRGISLFTGLLGTAFISAAAVSCYFAYFYNNFNYYYGGIGF
                     LIGILFYAFSWRKRNIAINAKKEIESMECEFADYTLSNNELNKQKDEILKRNNCQDFN
                     SMADKFQEKSKEKNIYEEKIKLLNYDDITFNEISADNKKIEERLNNNLKTLNLSLSED
                     TIIEINEAYKRKDTVKLQTEELNKKIEQINRDITRLDKEINFEQRRLEMILQANGADD
                     VEVFKKAVELNEKYKELLNRREYYEKILENIIGSTDYYELKKRTLNVSDEVKEIDKQD
                     IQLSIFKLNEEKTKLLQNINDIHHEIRDIEDNTRSLAEIEEEISFYEEKTAFFKNKIK
                     VAEIAAEKITKISDSIKGDFMPLLRSSISDNFSYLTGGKYKSVDIDENMNITVMSEEE
                     KDRKIELENLSGGTLDQLYFSLRIALSNILSGNQNIPLILDDSFIQYDSKRLRKSLEM
                     LSRESERRQVILFTCQEREAELSKQMNIKFNYFKL"
     gene            7761..8450
                     /locus_tag="PLB33_03135"
     CDS             7761..8450
                     /locus_tag="PLB33_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG62545.1"
                     /translation="MRIFAIADLHFDFKKEKPMDVFGDNWENHEEKIIENWKNTVDEE
                     DLVLIPGDISWALKLNDALEDLIRIENLPGMKIIGKGNHDYWWSTISQLDRLNLKTIK
                     FLKNNFHKYDDVVICGTRGWDTMEEYCAEVDNEKIFLRELNRFKISLQAAKKVSGKKI
                     IMLHYPPFNNNGLPNEFFSLMKDYNADICVYGHLHGEEGHRNVIEGLIDNIMVHCVSS
                     DYTGFKLKRIL"
     gene            8614..9801
                     /locus_tag="PLB33_03140"
     CDS             8614..9801
                     /locus_tag="PLB33_03140"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOG62546.1"
                     /translation="MFKAREYYSDILDEIKKSGLWKEERIITTPQRSIISTTKAGGVL
                     NMCANNYLGLADNEEIIEAAKNAYDKWGYGLSSVRFICGTQEIHKELEKKISEFLKTE
                     DTILYSSCFDANGGLFETITTENDAIISDELNHASIIDGVRLSKAKRYRYNNNDMESL
                     EEQLKQADKDGANIKLIATDGVFSMDGIIANLEGICDLADKYNALVMVDDSHAVGFVG
                     KTGRGTPEYRNVMGRVDIITGTLGKALGGASGGYTSGRKEIIDLLRQRSRPYLFSNTL
                     APAIVGASLKVLEMLMEDNKYKAKLDENTKYFREGIKKIGLNIIEGEHPIVPIMLGDA
                     VLSQKMAEKLLEKGVYVVGFYFPVVPKGKARIRTQISAAHSKEDLDFALNAFKDVKEE
                     LGI"
     gene            10328..11272
                     /locus_tag="PLB33_03145"
     CDS             10328..11272
                     /locus_tag="PLB33_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOG62547.1"
                     /translation="MSDKGLKEVKKTSIGGQALIEGIMMKGPSKIATAVRKPDGEIVI
                     KESNIKQIFKHKFFKLPLVRGSFVLIDSLVNGVKELMYSAEFYGEDYEEDALDRFLKK
                     VFKDKADTAIIYTSVIIALVFSIAIFILGPTILTNLLKNIIKNSFLLNLVEGIVRVSL
                     FVGYVYLVSKLEDIKRVFMYHGAEHKTIYCYEYGEELTVENVQRYSTLHPRCGTSFLI
                     NVLLISIIVFSFFGWPNPLLRLVIRLAMLPVIAGLSYELNRYVGRSSSDNIFTKIIAY
                     PGFLIQKITTKEPDDSMIEVAIAAMKRVIPQGGEDDRW"
     gene            11266..12117
                     /gene="prmC"
                     /locus_tag="PLB33_03150"
     CDS             11266..12117
                     /gene="prmC"
                     /locus_tag="PLB33_03150"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOG62548.1"
                     /translation="MVTIEKLLKDGIDIIKQREFNSPKLEVELILCYLLQKDRIYLHL
                     NRKEEVSEEIKDKFYKLAEKRNNGYPLQYITNSQEFMGLDFYVQEGVLIPRPDTETLV
                     ETVINIVKERYNKEIKILDLGTGSGAIALSLAYYLRNSFVTAIDISDIAVETTKINIK
                     KHNLNNIKVIKGNIFEDILGEKFDVLVSNPPYIESDEIKNLQTEVSVYEPRLALDGGV
                     DGLDYYKPIVELFKKICEENGVLAVEIGRQQKKSVEEIFLNSKVFKEVKAYRDLAGND
                     RVIIGMT"
     gene            12222..>13271
                     /gene="prfA"
                     /locus_tag="PLB33_03155"
     CDS             12222..>13271
                     /gene="prfA"
                     /locus_tag="PLB33_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOG62549.1"
                     /translation="MLDKLEALKDKYTELSEKLSDPDILSDSQKLQKLMKEQAQIEPI
                     VLKYNEYKDIRNQLEESKEMLKEKLDDDFKEMVKEEIKELSSKVEELENEIKILLLPT
                     DPNDEKDVIVEIRAGVGGDEAGLFAGDLLRMYLRYAERRGWKTEFISVNEQGIGGYKE
                     VIFSIKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSSATVAVLPEADDIDIEINPN
                     DVKVDVFRSSGNGGQSVNTTDSAVRLTHIPTGIVISCQDEKSQLKNKDKAFKVLKARL
                     YDKYLQEQTDELAQSRRSQVGSGDRSERIRTYNFPQGRVSDHRIGLTLYKLDAFLDGD
                     ITEMIDALITSDQAEK"
     assembly_gap    13272..13371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13372..13591
                     /locus_tag="PLB33_03160"
     CDS             <13372..13591
                     /locus_tag="PLB33_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOG62550.1"
                     /translation="ARIIKRRTIAKEACEQGRVLINGKIAKPGAEVEIGDEIDIIFGT
                     KPMKIKVEKLLEHTVKDEYKQMYSIINV"
     gene            13684..14220
                     /locus_tag="PLB33_03165"
     CDS             13684..14220
                     /locus_tag="PLB33_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOG62551.1"
                     /translation="MELKGSKTEQNLMTAFAGEAQAHVKYTYYQSQAKKDGYVQISKY
                     FEETARNEKEHAKLWFKLLHDGIAHTSVNLQDAADGEHYEWTEMYAEFAKVAREEGFD
                     KIAYLFEGVAAIEKAHEERYLALLNNIETGKVFKREGEVVWQCDNCGHIHVGSQAPKV
                     CPVCDHPQAHFQLYTKPY"
     gene            14362..15288
                     /gene="murB"
                     /locus_tag="PLB33_03170"
     CDS             14362..15288
                     /gene="murB"
                     /locus_tag="PLB33_03170"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOG62552.1"
                     /translation="MEYQLIINSMLDELTQSFRGLVLKDEPLKNHTYFKIGGPASIFA
                     IPEDTEDLKTVLKLIKKYEIDYYIIGNGTNLLVSDEGFKGIIIKIGEKFNKLSRDGNK
                     VTAGAGMLLSTLAKYFAKEGLEGFEFAGGIPGYLGGAIPINAGAYGGEMKDVVTKVKC
                     MDMDGRIHEFSNEEMEFEYRHSRLSDSEYIVLEAEISLTPGRVEDIMEKMKELNEKRI
                     SKQPLNLPSAGSTFKRPLNGYASKLIEDAGLKGLKYRGAMVSDKHSGFIVSYDNASCE
                     DVLELMRIVISTVYDKFGIILEPEIKIVGTKL"
     gene            15329..16069
                     /locus_tag="PLB33_03175"
     CDS             15329..16069
                     /locus_tag="PLB33_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOG62553.1"
                     /translation="MKLRADYHIHSTYSKNRHGKSSIEEIAERAYELGLEEIAIADHG
                     PAHYLFGIKSNLISEAKNIVVETRKKYPNLKILYGVEANILDYKGTTDINSEILKHCD
                     IILCGFHLAALFSSPSDFWNFIVKNQLAKNNKKFYDEMVEKNTQAIINAMNKYKINIL
                     THPGDKIPVNIDKIAQAAEKTDTMLEINNSHGHLNKDEIIRASKYNVQFVINSDSHIK
                     DNVGRCDNALRAVEGSGIDLNRIVNLKS"
     gene            16081..16938
                     /gene="rapZ"
                     /locus_tag="PLB33_03180"
     CDS             16081..16938
                     /gene="rapZ"
                     /locus_tag="PLB33_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOG62554.1"
                     /translation="MELVIITGMSGAGKSQAIKVLEDINYYCMDNLPPALLPNFAELC
                     KSSSMDVNKVAVVADIRGGIFFKDLFNSLKELKNKGIEYRILFLDASDDDLIKRFKEQ
                     RRPHPLSSDGTIIEGILEERNRLEEVKKKSDYIIDTTSMTLGRLKEEILNIFVKGSIS
                     HNLNITIVSFGYKYGLPKESDLVFDVRFLPNPFYIDELKNSTGNDENVKDYVMSFETT
                     SIFLEKLMDILKFLLPLYIKEGKSSLVISIGCTGGKHRSVTISNYLASMLTAENYRVL
                     LNHRDAGKN"
     gene            16939..17904
                     /gene="yvcK"
                     /locus_tag="PLB33_03185"
     CDS             16939..17904
                     /gene="yvcK"
                     /locus_tag="PLB33_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOG62555.1"
                     /translation="MKIVVFGGGTGLSVLLRGLKNYTKDISAIVTVADNGGGSGVLRE
                     DLGMLPPGDMRNCIIALSDIEPIMIKLMQHRFSDGYLKGQSFGNLFIAAMYEIFGNYE
                     HALKEIGSIFRLSGKVLPMTLQDAQLKAYLANGDCILGEKYIPEYVSKLNTKIDKIEL
                     VPYVCKPLEETVEDIKEADLIVLGPGSLYTSVIPNILVEGIPSLLAESNATVFYVCNL
                     MTQPGETDNYNVLDHVNAIIKHSMKYMIDYVIVNDEKISEELILNYKYQGAKQVLIDE
                     KQIKELEEMNIKVICSNFVDIRKNYIRHNSDKIGQLLLNYINEKK"
     gene            17919..19004
                     /locus_tag="PLB33_03190"
     CDS             17919..19004
                     /locus_tag="PLB33_03190"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOG62556.1"
                     /translation="MEKYKSIDPNQLSIADGMIDWVRVVSKDNMVLYANKKMQEDLGE
                     NIVGKKCYDVLCRYNRCENCISSTTLEKGSIMRKHHKLGNKTYMIISSPLFDDKGEIE
                     ASVEVFRDITNEVLLSEKLQEKNKKMSDDIKFAKNMQNRMLPPKGMYNGLSIDYLYES
                     SESLSGDYFDVFEIDENNTGIYICDVVGHGVTASLLTIFVRQSLRTIAKGNINLNKIM
                     KELHKTFLSLNLDYDKYFSVFFGIYNKDSHVFKYVNAGHNSVPILISGKDIKKLMLKG
                     YPIANIFDNVDYIVEKVTLKVGDKILLYTDGITETTNSEGVQFGVDRLINTIISDGNI
                     LENIKSSVNNFSIVHKDDYAVLLAEII"
     gene            19116..20411
                     /locus_tag="PLB33_03195"
     CDS             19116..20411
                     /locus_tag="PLB33_03195"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOG62557.1"
                     /translation="MKNITLDLRGSFIDEDELKKIKPEIMAAQETLLDRKGVSSEMTG
                     WLDYMYRIDKNEYEEIKAAAKYIKENSEAFILLGIGGSYLGAKAAIEAIRGDFHNELL
                     SPKVYYAGQNLSGAYLKKLIELVKEKEVCIIVISKSGTTLETALSFRVFKELIEKKYG
                     EESKNRIFVVTDKYKGALKSISDLKGYKTFVVPDDIGGRYSVITPVGLLPMAAAGLDT
                     DKFMEGLISGAEEYSVRDFECNISLLYTAARNILNRRNKEIEIFVNYEPSCISIAEWW
                     KQLYGESEGKEGKGIFPASLNFTTDLHSMGQFVQEGREIIIETTLLIENNEDDMVIPF
                     DLNDFDKLNYISGKTFNFVNLKAFEGTFMAHNEGKVPGIIISVPKMDEFYLAKLFYFF
                     MMSCGISGYTNKVDPFTQPGVEAYKKNMFKLLKEEEHTK"
     gene            20423..21322
                     /locus_tag="PLB33_03200"
     CDS             20423..21322
                     /locus_tag="PLB33_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOG62558.1"
                     /translation="MIKLGEMQELEVAKIVNIGAYLQSEDKEKTEDRVLLPIKQVPAG
                     TKVGDKINVFVYRDSDDKIIATVKKPKITLGEIATLKVVEMSRIGAFLNWGLEKDLFL
                     PFKEQIGDIRLNGEYMVGLYIDKSDRLCATMNLFKVLRTDSPYKVNDIVKGTVFSLKR
                     GLGAMVAVDNKYLGLIHEGEIIKPLHPGQSVEVRVSNIKEDGKLDLSLKDAPRLQIDK
                     DGEKILKVLIRNKGSLPLNDDSSPEEVNKILGMSKSSFKKAAGRLMKRRLIIMTKNGI
                     EMLSDDEKQQSKVKKSNIRYKKK"
     gene            21382..22083
                     /gene="deoD"
                     /locus_tag="PLB33_03205"
     CDS             21382..22083
                     /gene="deoD"
                     /locus_tag="PLB33_03205"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOG62559.1"
                     /translation="MSTPHNSAVIGQIAETVLLPGDPLRAKFIAENFLEDVEQFNTVR
                     NMYGFTGSYKGKKVSVMGSGMGCASIGIYSYELIHMYGVKNLIRVGSCGAYAPDLNLY
                     DIVLAIGASTDSNYAYQYELPGIYSATASWELLSKAKKVADDNNIKAVVGNIVTTDIF
                     YHDNSENWRKWAKMGCLAVEMETYALYCNANRAGVNALTILTVSDSLEHKTETTPEQR
                     EKSFTDMIKIALEIL"
     gene            complement(22157..22357)
                     /locus_tag="PLB33_03210"
     CDS             complement(22157..22357)
                     /locus_tag="PLB33_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG62560.1"
                     /translation="MKTGTVKWFDSAKGFGFITSDEGNDVFVHFSAINADGFKTLEEG
                     QKVTFEVVEGTRGPQAANVEII"
     gene            complement(22504..22704)
                     /locus_tag="PLB33_03215"
     CDS             complement(22504..22704)
                     /locus_tag="PLB33_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG62561.1"
                     /translation="MKTGTVKWFDSKKGFGFISCDDGKDVFVHFSAINSDGFKTLEEG
                     QKVNFDIVDGQKGPQASNVTVA"
     gene            22920..>23691
                     /locus_tag="PLB33_03220"
     CDS             22920..>23691
                     /locus_tag="PLB33_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOG62562.1"
                     /translation="MIKHFLKVFFASFLLIAIILSVTALGYIFLVDKDIVIGKIKFVN
                     DEKSEVSFYESSSFDYGTSIGKAASNMKRFNILLAGLENQRTDTIIALSYDMESKTAD
                     LISLPRDTYLPRNADDNAELKKINAIYSHEGIEGLALRVQELLGIPIEKYVVIDYEGV
                     IACVDMIGGVEVNVPFHMEYTDPYDDPPLYIDIPEGVQLLNGEQSLKFLRYRKGYDNQ
                     DLGRIEAQQQFIKSAVKKTLGFQLPAVIKEAYSHIETNI"
BASE COUNT         8836 a         3335 c         4803 g         6517 t
ORIGIN      
        1 agttattatt gctattaata tttaaagcgt tgaagcagag aaaagtattt cagacgttta
       61 cagagaattg gcggtggtga gagccagtaa cagaggaata taatatacac tgctgagcag
      121 gtttgctaaa aggaactccg agtaggcgaa cacgtgtact cacgttacag ggttagaagt
      181 tgttagactt cgaagagtga tgatttatat cataattagg gtggtaccgc ggaaatcttt
      241 cgtccctata agcatatttt aaaaaagtat gtttatagga atgaaagatt ttttttatga
      301 tttacctgta atcatgtttt ttcgatatcg aaccctggcc aagttcatat ctaaaaataa
      361 ataaaccttg gagggacatt atgaaaaata gtacaattaa atttatagca attttatttc
      421 tggtttcgct gataataacc ggatgttctg caaatacaga agcaaacaag cctgcaggag
      481 gaaaatttac aataggaata gcacagcttg tgcaacatcc tgccttggat gcttcaagac
      541 agggatttat agatgagatg gaaaagttgg gtgtagaagt tgaatttatt gaccagaatg
      601 ctcaggcaga cataaacaat gcacaaatga ttgcagaaaa atttgttaaa gatgatgtgg
      661 atttaatatt ttcgattgca acacttacat ctcaggcagc aaaaaaagca attgaaggaa
      721 caaagatacc attggtattt actgctgtaa cagaccctgt tttctctgaa ctggtaacag
      781 ctttggatgt tacagacaac aatattactg gagtagttga tgcagctccc ataaaggaaa
      841 accttgagtt gtttaaactg ttaaaggaag atataaagac agtgggaata atttacaata
      901 ttggagaatc aaactctgaa gttcaagttg aacagacaaa agaaattgca gcagaacttg
      961 gactgacaat cgaaacagtc gggattactt caattaatga cataccgcaa gcagttgaca
     1021 ccatttcaaa aaaggtggaa ggtctatata taattacgga taacatggtg gcatcagcta
     1081 tggaattagt tgccaagcta gccggagaaa aagatttgtt gactgtatcc gccgatggca
     1141 gtcatgtaga ccaggggata ctgatttcaa agggtataag ctactacgta ataggtcagc
     1201 aatctgcaag gatagctaaa caaatattgg ttgataaagt tgatgttgct gacattactg
     1261 ttcaagcatc aaccgaattc gagaaaaaag ttaatttaaa aacagcagaa agtctgggac
     1321 tggcaaaaga acataaagca tttgaaggag cagaatttgt tgaatgaata cattaattct
     1381 tacatcaatt gaacaaggtt taattttttc cgtattagca ataggagtta ttattacaat
     1441 taaaatactt gattttgcag atatgtccgt tgaaggaaca ttcccgatgg gagcatttat
     1501 atttgctaaa ttcattacca caggcttctc tcctattata agcacgcttt taacctttat
     1561 cctgggaact ttggcaggac tgttaacata cacattaaac attaaactaa gaataagagc
     1621 tttgttatcg gggattctta cagctaccat attatattcg gcaaacttaa gattaaacgg
     1681 cagatcaaat atagggctaa caagctatga ttcaatattt gacggattcc gacccgtaat
     1741 agtgcttata ctgatagttc ttgcagtaaa gatattaatg gatatatttt taagaaccga
     1801 agtggggtat ttgttaatag caacaggaga caatgaaaca ttggttaaat ccttgggaga
     1861 gaattcaaac aaatacaaat tgataggatt aatgctttca aacggattag tatcgttaag
     1921 cggagctctt atggcacaat accagggttt ttcggaaata tcaatgggaa ccgggataat
     1981 agtggtggca atagcttcaa taattgtggg cgatacagta ttaagaaaca gcaaaatttt
     2041 aaaaatgacg acaagggcaa ttatgggagc cttaatatat aaattcattg gagccctagc
     2101 aattgattta ggccttgcac cgacagacct gaaagcaata aatgcggcaa tagtaattat
     2161 atttttgagc tacaatacgt tcagccctgt gaaaatgaga aaggcaagaa aaataaaagc
     2221 cgtggaggga gtaaaatgtt agatataata aatttatcga agactttcaa ccaaggaaca
     2281 gattatgaga taaggatatt cagtaatttc aacattcata taaacaaggg agaatgcacg
     2341 gcaatattgg gagcaaacgg atgcgggaaa agcaccttgt ttaacataat aagcggcttt
     2401 ttaatgcagg agaaaggtat tataaaatta aacgggaaaa atatcaacgg tttaaaagaa
     2461 aacgaaaggg ctaagtttat aggaagagtg catcagaatc catcggcagg cgtttcaccg
     2521 tcactcacaa tattggaaaa tataagcctg tcggataaga agtgtcagaa atttacatta
     2581 agaaaattaa tcaggaaaaa taaaatcgaa gcttataaag aaattttaag aactcttgac
     2641 ttaggacttg aaaccaaatt ggatacggaa gtgaaatacc tttcaggagg gcaaaggcag
     2701 tcactgtcct tgctgatggc aacaatgaat aaaccgcagc ttcttttgtt ggatgagcac
     2761 acagcagcct tagaccccaa aacctcaagg cttgtaatgg aaaaaactca ggaattaata
     2821 aaaactcaaa atattacaac tatgatgata tctcacaatg caagagatgc cctaatgtat
     2881 tcggatagaa ttattatgct tgacaaggga gaaataattc ttgatctaag aaaggggcag
     2941 ataacggaga aggagttact gaatatttat aactctagaa attacggaag cccgctgacg
     3001 gaagcagtat agctaaggga aggataataa ttatccttcc tttttattgt ttttgacacc
     3061 taaatgatag tattgtataa ttatgttatt gacgaccacg agttattcat caaagacaga
     3121 tgaagaaagg ttttattatg gaagatgtta ttaaagaaat taaaaataat ccgctgtttt
     3181 tcaatatgag cgaggatgaa ataaaatttg ttcttaaatg cagtaattca accgttaaag
     3241 atttaaggga caattatgct ttatttgaaa aggaccaaaa agtaaagaag cttggaattg
     3301 taattgatgg aatattaaat ctggaatccc gaaaatataa cggcaacagg gttatagtaa
     3361 caacattgga gaaaaatgac ctattcggag aagctttaat ctatgcttcc gaaggtagtt
     3421 ctccctatga ccttgtgagt tcaggaaaca gcaaagtatt gctaataccc tataaaatct
     3481 tttccaatat gtgctttaac aactgcagct tccatgctca attgataaaa aacatgctta
     3541 ccattttatc cgataaaata gttatgctca ataataaaat gaatattcta aatgccgaaa
     3601 cattaaaggg cagaattgca gtatatcttt tgtctcttca aaaaaaatca aattccatga
     3661 tatttaacat gcccatgaac agacaagaac ttgccgagtt tttaaatgtt aaaagaccat
     3721 ctctgtcaag ggagctttcc aacatgcaaa aggaaaaaat tattgaagtt taccgctctt
     3781 ccgttaaaat attggacatg gaaaaactct gtgaattnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnntat tgaagatgta tactatctgt ggtaaaatat tatatattca
     3961 cataaatcga aggagctgct atgaaactta aatttataca tacatctgac ctgcatttag
     4021 ggaaaaaatt cgatattaaa aatttcagtt taaaagagcg caaaaaaaga agacaggaat
     4081 tatgggattc ttttgatgaa attataagga cagccaaaat tgaggaagta caatatttgt
     4141 ttattgcagg ggatttaacg gacggagaat atataagcta taaaagttta aataaaattt
     4201 cttcgaaatt cagctccata ccggacacaa atgtaataat tacctgcggt caaagcgacc
     4261 cttataatgt taacaatatg tatgaatata tagaatggcc ggataacgta tacataatta
     4321 aaaataccga aacagttgag aaactgaatt ttcccgaaaa caatttgtgc atctattcaa
     4381 taagttggga caaccatgca gaaaataaaa gaacccagcc catttacgac atatcagttg
     4441 atgaaaacaa gctcaacatt ctcatgcttc attgcgatat cgattctagc gacagattgc
     4501 cggttaatat agacataata aagaataagt ttgattattg tcctcttgga ggaagacata
     4561 acttccgtca aataacggac aatgttgtgt attgcggtac tcctgagcct ttgtcttttg
     4621 atgaggaagg cgaacatggg attataaggg gaaccttgga aaaaaataat acacaatact
     4681 catttttacc gattgcaaaa agaaaattta taaatagaca aattgaatta aatatttcat
     4741 acagctttaa taaaatattg gatttgatta aattttccgg cgatactttt tccaacatta
     4801 aggattatgt gcgcatagag cttacgggtg aagtaaatgc cgatatttca atggatgaaa
     4861 ttgaaaatga ggcaaaacag tttttttatt atatagaatt tgaagacaat tatatatatg
     4921 atacagaaaa cagaaattat gaaggcaatg aattcaatat tattgaaagc tataaacttc
     4981 aatttgacaa aaatgacgat aatgacgata aattacagca gcaggcattt aaactgggac
     5041 ttgaagttct cagaaaagaa aaggtggtaa aataaaatgt acataaacag actgaattta
     5101 agagcctttg gaaagtttat caacaggaaa atacatttta gcaataaatt taatattatc
     5161 ttcggtgaaa atgaaacagg aaaatctacc atacataatt ttattgagtt ggttatgtat
     5221 ggatttgatg aagatacatc tcggtataat aaatataaac cttggaacag ccccgtgtac
     5281 aagggaagca ttgaaatagc ggatgaagag aaaaaacata taatttcaag agattttcta
     5341 ttagatacta tacaggtttt caaaaaaaca aatgaaacaa aagaaaatga atattcggtt
     5401 gagggtatcg gtgttccggg tgagcatttc tttaatataa acagagtatc atttaacaat
     5461 acagtaagta tcagtcagct tggaaataaa acagaaaagg aattggctga tgagctgaaa
     5521 aataaaataa caaatttaag caatacaaaa gatgaagata tttcaatcga cagaattatg
     5581 caaagcctgt atagaatcaa agaagaagcg ggaagcgaaa ataatgataa aacactgctg
     5641 ggtcagtatt cgcttaggtt ggaagagctc agaaatgcca aagaaagttc aatgaattca
     5701 aacagacagg taatgttttt ggcaatggaa aagaaaaagc ttcaaagtaa aattcatgag
     5761 ctgaatatga aaattgaaga aaaacaaaag gaaatttcag attatgaact ttttttggag
     5821 aagcaaaaat tcctgagagc tcagcctgta aagaaggaaa tagacaatat caacgatgaa
     5881 cttaaattaa ttgaagatga tgttacgaat gtatcagcgg aggattacaa agaagcgtct
     5941 gaaacagaaa gcagtctggc ttcaatgaaa aatgataagc aaagattgat tgaagaaaaa
     6001 gaagaatgca tttcagagct tgaattaata gaatctgatt tgtctaatta tattcccgaa
     6061 gagtttgata tagataagtt aaatatcgat tatgacatat ataaggcaaa taacgaaaag
     6121 atagacactc ttaaaatgaa gatgaaaact ggcagggaga gcataaattc aattaatata
     6181 gacgaaatta acaattttat taatgactac agggaagttg aagagctcaa tcataaaata
     6241 gaaattaata atgtcttaac ggatagcaaa aattatgaat tgatgaagaa attcggcaaa
     6301 aaacaaagag ggataagcct ttttacggga cttttgggaa cggcattcat atcggctgcg
     6361 gcagtatcat gctactttgc ttacttttac aataatttca actattatta cggcggaata
     6421 ggttttttga tagggatctt attctatgct ttttcatgga gaaaaagaaa tattgcaata
     6481 aacgcaaaaa aagaaattga aagtatggaa tgcgagtttg ctgattatac attatcaaac
     6541 aatgaattaa ataaacaaaa agatgaaata ttaaaaagaa acaattgtca agactttaat
     6601 agtatggctg ataaatttca agagaaaagt aaggaaaaaa acatttacga agaaaaaatc
     6661 aagcttctta attatgatga tattactttc aatgaaattt cagcagataa taaaaaaata
     6721 gaagaaaggc tcaataacaa tttaaagact ttaaacttat ccttgtcaga agatactata
     6781 attgaaataa atgaagctta taagcgtaaa gacacggtaa agctccaaac agaagaatta
     6841 aacaaaaaaa tcgaacaaat caaccgcgat ataacaaggc tcgataagga aataaacttt
     6901 gagcaaagac gcttggaaat gatacttcaa gcaaacggtg cggatgatgt agaagtattt
     6961 aagaaagcag ttgagctgaa tgaaaaatac aaggagcttt taaaccgcag ggaatattac
     7021 gagaaaattt tggaaaatat aattggcagc accgactatt acgagcttaa aaaaaggact
     7081 ttgaatgttt ccgatgaagt caaagaaatt gacaagcaag atattcaact tagtattttc
     7141 aagttaaatg aagaaaaaac aaagctgtta caaaatataa acgatataca tcatgaaata
     7201 cgagatattg aagacaacac aagaagttta gcggaaattg aagaggaaat cagcttttat
     7261 gaagaaaaaa ccgctttctt taaaaataaa atcaaagtag ctgaaattgc agcagaaaaa
     7321 ataacgaaga tatcagattc aataaaaggt gactttatgc ctcttcttag aagctcaata
     7381 agcgataatt tttcatactt gacaggcggg aaatataaaa gtgttgacat tgatgaaaat
     7441 atgaatatta cggttatgtc tgaggaagaa aaggacagga aaatagagct tgaaaattta
     7501 agcggaggta ccttggacca gctttatttt tcactgcgta tagccctaag caatatttta
     7561 agcggcaacc agaatatccc gctgattttg gatgacagct ttattcaata tgattcaaaa
     7621 aggcttagaa agtcattaga aatgctttca agagaaagtg agagaagaca ggtaattctc
     7681 tttacatgcc aggaaagaga agcagagctt tcaaagcaaa tgaatataaa attcaactat
     7741 tttaaactat aaggataatt atgagaattt ttgctatcgc tgatttacat tttgacttta
     7801 aaaaagaaaa gccaatggat gtatttggcg acaattggga aaaccatgag gaaaagataa
     7861 ttgaaaactg gaaaaatacc gtagatgaag aagatttggt tctaattccc ggtgatatat
     7921 catgggcttt aaagttaaat gatgccttag aggatttaat cagaatcgag aatttacccg
     7981 gcatgaaaat tataggtaag ggaaaccatg attattggtg gtcaactatc tcccaactag
     8041 acagactgaa tttgaaaact ataaagtttt taaaaaacaa ttttcataaa tacgatgatg
     8101 ttgttatttg cggtaccaga ggttgggata caatggaaga gtattgtgcg gaagttgaca
     8161 atgaaaaaat atttttaaga gaattgaaca gatttaaaat ttcccttcaa gcagcaaaaa
     8221 aggtcagcgg caaaaaaata attatgcttc attatcctcc ctttaacaat aacggacttc
     8281 ctaacgaatt tttttcactg atgaaagact ataatgcaga tatttgcgtt tacggccatc
     8341 ttcacgggga agaagggcat agaaatgtaa tagagggttt gatagacaat attatggtcc
     8401 attgtgtttc aagcgactac accggattta agctgaaaag aatattatga caaggggacg
     8461 ggggtgctgt catcaaatat tgtatacaat agatggacaa actatctaat cggctcttga
     8521 cataaaaaca taacaagagt attatttgga taaagtaata agattgaagc ggaaaattgt
     8581 actgaacaaa gaataaagat gggaggaaac agaatgttta aagcaaggga atactattcc
     8641 gacatattgg acgaaattaa aaaaagcggt ctttggaagg aagaaagaat tataaccaca
     8701 cctcaaagaa gtattatcag cacaacgaaa gcaggcggag tactgaatat gtgtgccaat
     8761 aactatttag gattggcgga caatgaggaa ataatcgagg cagcgaagaa tgcatatgac
     8821 aaatggggtt acggactttc ttcagttaga ttcatttgtg gcacccaaga aatacataaa
     8881 gagcttgaaa aaaagataag cgaatttctt aaaaccgaag ataccatttt gtattcatca
     8941 tgttttgatg caaacggcgg cttgtttgaa acaataacca ccgaaaatga tgccataata
     9001 agcgacgagc tgaaccatgc aagcattatt gacggagtaa gattatcgaa agctaaaaga
     9061 tacagatata ataacaatga tatggaatca ttggaggaac agctgaaaca ggctgacaag
     9121 gatggagcca atattaaatt gatagcaaca gacggtgtat tctcaatgga cggtattatt
     9181 gcaaatttag agggtatatg cgatttagct gataaatata atgcacttgt aatggttgac
     9241 gacagccatg cagtgggatt tgtaggtaaa acgggaagag gaactcctga atacagaaat
     9301 gtaatgggca gagtcgatat tattacggga accttaggaa aggctcttgg cggtgccagc
     9361 ggcggataca caagcggcag aaaggaaata atagatttat tgagacagcg ctcaagacca
     9421 tatttgtttt caaatacatt ggcgccggct attgtcggag catcactcaa ggttcttgaa
     9481 atgctgatgg aagacaataa atataaggct aagctggatg aaaatacgaa atatttcaga
     9541 gagggaatca aaaaaatagg tcttaatatt atagaaggcg agcacccgat agtgcccata
     9601 atgcttggag atgcggtctt atcccaaaaa atggctgaaa agctgttaga aaagggtgtt
     9661 tatgtagtag gtttttactt cccggttgtg ccaaagggga aagcaagaat aagaactcaa
     9721 atatccgcag cccattcaaa ggaagattta gactttgcat tgaatgcatt caaagacgta
     9781 aaagaagaat taggcatata gaaaaaagat aattcttaag taaaataagc tcaaaaattc
     9841 aagtaatgtg tgatttgtaa taaagaaatt cgaattattc acacattact ttaatttttg
     9901 tacattatgg gaaaatatta aaggaaatat tagccgctat tattaaaaaa acattaagct
     9961 ttgcagtgta taattagaaa gcttcaggct attaacaatt aaaaacttga agaaatgaaa
    10021 ttttaattgt ataataagat gccctaaccc catttattca aggtatgggg acacacctcc
    10081 ctaattttgg gaaggtctct gtttgctgga ggaatgtccc caaattaggt tttcagaaaa
    10141 atggcggtag gtcattcgca acgaattccc aaaatatgcg accggaggga gctgatattt
    10201 tggtgaatga gactgcgtat tcgttgtgtt ctattacatt gtttttacag tgtttaacaa
    10261 ttttctacat ttctcgggaa ataatcctaa aatgtgagtt gattctatat tttataaagg
    10321 ggacgaaatg agtgataaag ggttgaagga agtaaaaaag acaagtatag gcggtcaggc
    10381 gcttattgaa gggatcatga tgaagggacc ttcaaaaata gctacagccg taagaaaacc
    10441 tgacggagaa atagtaatta aagagagtaa tataaaacaa atatttaaac ataaattttt
    10501 taaattaccg ctggtaagag gaagctttgt gctcatagat tcactggtaa acggtgttaa
    10561 ggaattaatg tattcggctg aattctatgg tgaagattat gaagaagatg cccttgacag
    10621 gtttttgaag aaagtattta aggataaggc agacacggca attatttata cttcggtaat
    10681 aatagcctta gtattttcga ttgcaatatt tatattgggt ccaaccattt taacaaattt
    10741 gttaaaaaac attattaaga attccttttt gcttaacctg gtggaaggca tcgtaagagt
    10801 atccttgttt gtaggttatg tttatttggt ttcaaaactt gaagatataa aaagggtatt
    10861 tatgtatcac ggggctgaac ataaaaccat atactgttat gaatacggcg aagaattgac
    10921 tgttgaaaat gtccaaagat attcaacact tcaccctcgt tgcggaacaa gttttttgat
    10981 aaatgtattg ctgataagca ttatagtatt ttcatttttc ggttggccta atccgcttct
    11041 gagacttgtg ataagacttg cgatgcttcc ggttattgca ggactttcct atgaattaaa
    11101 cagatatgtg ggaagaagct caagcgataa tatttttaca aaaattattg cttatcccgg
    11161 atttttgatt caaaaaatta ccactaagga gccggatgat tcaatgattg aagttgcaat
    11221 agcagcaatg aagagagtaa ttccccaagg cggagaagat gatagatggt aacaattgaa
    11281 aaattattga aagacggtat tgatataata aaacaaaggg aatttaattc tccaaagctt
    11341 gaagttgagc ttattttatg ttatctttta caaaaggaca gaatttatct tcatcttaac
    11401 agaaaggaag aagtaagcga agaaattaaa gataagtttt ataaattggc tgaaaagagg
    11461 aataatggtt acccgctgca atatataact aattctcagg aatttatggg cttggacttt
    11521 tacgttcagg agggtgtcct gataccgaga cccgatactg aaacattggt agaaacggta
    11581 ataaatatag taaaagaaag atataataaa gagattaaaa tacttgattt gggaacaggc
    11641 agcggagcaa ttgcattaag ccttgcttat tatttaagga actcgtttgt taccgcaata
    11701 gacataagcg atatagctgt tgaaacgaca aaaataaata taaaaaaaca caatctaaat
    11761 aatataaagg ttataaaagg aaatattttt gaagatattt tgggtgaaaa atttgatgtt
    11821 cttgtatcaa atcctccata tatcgaaagt gatgaaatta aaaatcttca gacagaggtt
    11881 tcggtctatg agccaaggtt agctttggac ggaggagttg acgggttgga ttattacaaa
    11941 cctattgttg aattatttaa aaaaatttgt gaggagaatg gagtattagc tgtggaaata
    12001 ggaagacagc aaaaaaaatc agttgaagaa atctttctga attctaaagt gtttaaagaa
    12061 gttaaagcat accgggactt agccggaaat gacagggtaa taataggtat gacttagcat
    12121 cgcttattaa ttatggagac acaccgaaga atctcatgag atccttcgct gtgctcggat
    12181 gacataacaa acatattaga tttataagag agaggaataa aatgttagat aaattagaag
    12241 cattaaaaga taaatataca gaattaagcg aaaaactatc tgaccctgat atattaagcg
    12301 attcacagaa gctgcaaaag cttatgaaag agcaggcgca gatagaacct attgtgctta
    12361 aatataatga atataaggat ataagaaacc agcttgaaga atccaaggaa atgctgaagg
    12421 aaaaattaga tgatgacttt aaggaaatgg taaaagaaga aattaaggaa ttatcttcta
    12481 aagtagaaga gctggaaaat gaaataaaaa ttctgcttct gccgactgac ccgaacgatg
    12541 aaaaggatgt aattgttgag ataagagcag gagtgggcgg tgacgaagcc ggattatttg
    12601 caggcgattt acttcgcatg tatttaagat atgcagaaag aagaggatgg aagactgaat
    12661 tcatttcagt aaatgaacaa ggaataggcg gttacaagga agtaattttc tctattaaag
    12721 gaaaaggagc atactcaagg cttaaatatg aaagcggtgt tcacagggtg cagagagttc
    12781 ccgatacaga atccagcggc agaatccaca cctcatctgc aaccgtagca gttctgcctg
    12841 aagcagatga tattgatatc gaaataaatc caaatgatgt aaaggttgat gtttttcgct
    12901 cttcgggaaa cggaggacag tctgtaaata caactgactc tgccgtaaga ctaacccaca
    12961 taccaaccgg tattgttatt tcatgccagg acgaaaaatc tcagcttaaa aataaggata
    13021 aagctttcaa ggttttaaag gcgagacttt atgataaata tttgcaagag caaactgatg
    13081 aattggctca atcaagaaga agtcaggtgg gctccggaga cagaagcgag aggataagaa
    13141 cttataactt ccctcaggga agggtaagcg accacaggat aggtcttact ttgtataagc
    13201 ttgatgcatt tcttgacgga gatataactg agatgattga tgcacttatt acttcagacc
    13261 aagcggagaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcacgcat
    13381 aattaaaaga agaaccattg ccaaagaagc atgcgagcag ggaagagttc ttattaacgg
    13441 caagatagca aagcccggtg cggaagtaga aatcggcgat gaaatagata taatattcgg
    13501 cacaaaaccg atgaaaataa aggtagaaaa actattagaa catacagtca aagatgaata
    13561 caaacaaatg tacagcataa taaatgttta atcagattat cagggtatga gttaaaattg
    13621 gagagtttga tatctaagca gccatgctgc tcacataaga taaatattta aagaggtggt
    13681 tatatggaat taaaagggag taagactgaa caaaatttga tgactgcatt tgcaggcgaa
    13741 gctcaggctc atgtgaagta cacatattat caaagccaag ctaaaaaaga cggttatgta
    13801 caaatcagca aatactttga agaaacggca agaaatgaaa aagagcatgc aaaattatgg
    13861 tttaaacttc ttcatgacgg aatagcacac acaagcgtaa acttacagga tgcagcagac
    13921 ggtgaacact acgaatggac agaaatgtat gcagaatttg caaaagtggc aagggaagaa
    13981 ggattcgata aaattgccta cttatttgaa ggagttgcag cgatagaaaa agctcacgaa
    14041 gaaagatact tagctttgtt gaataatatt gagacaggca aagtattcaa aagagaaggg
    14101 gaagttgttt ggcagtgcga taactgcggc catatccatg ttggctccca agcccccaag
    14161 gtatgcccgg tttgcgatca ccctcaggct catttccaat tgtacacaaa accctactaa
    14221 aattagaaaa ttgccattca atatggcaat ttttttgttc tgataaaata taattaccat
    14281 tatgccatat taaaaataaa tctttaaaaa taatcaattc aatgttagaa tatacatagt
    14341 tgatagggaa agggtggaat aatggaatat caattgataa tcaacagcat gctggatgaa
    14401 ttgacacaaa gtttcagggg acttgtgctg aaagatgagc cgttaaaaaa tcatacttac
    14461 ttcaaaatcg gaggtcctgc aagcatattt gcaatacctg aagacacaga agatttaaaa
    14521 acagttttaa agctgataaa gaaatatgaa atagattatt atattatagg aaacggaaca
    14581 aatctcctgg tttcggacga ggggtttaag gggataataa ttaaaatagg ggaaaagttc
    14641 aacaagctta gtcgtgatgg aaataaagta acagcaggag caggaatgct tttgtccacc
    14701 ttggcaaagt attttgcaaa ggaaggcttg gaaggttttg aatttgcagg aggaatcccc
    14761 ggatatttgg gaggagcaat tccaataaat gcaggagctt acggcgggga aatgaaggat
    14821 gtggtaacga aggtcaaatg catggatatg gacggcagaa ttcatgaatt ttccaatgaa
    14881 gaaatggaat ttgaatatag acattccaga ctgtccgatt cggaatacat tgttcttgaa
    14941 gcagagataa gtttaacacc cggcagagtt gaagatataa tggaaaaaat gaaggagctt
    15001 aatgaaaaga ggatttcgaa gcagccatta aatttaccaa gtgccggaag cacatttaag
    15061 agaccattaa acggatatgc ttccaaatta atagaagatg caggacttaa agggctgaaa
    15121 tacagaggag caatggtttc agacaaacac agcggcttta tcgttagtta tgataacgca
    15181 tcttgtgaag atgtcctgga gcttatgaga atagtaataa gtaccgtata tgacaaattt
    15241 ggaataatat tagaacctga gataaaaata gtaggaacga agttgtagaa gataaagtaa
    15301 aacatataaa ataacaagga agttttaaat gaaactaaga gctgattatc acatacactc
    15361 aacatattca aaaaataggc acggcaaatc atcaattgaa gaaatagcag aaagagccta
    15421 tgaactggga ctggaggaaa tagccatcgc tgaccatggt cctgctcact atttgtttgg
    15481 tataaaaagt aatttaataa gtgaagctaa aaacattgtt gtggaaacga gaaaaaagta
    15541 tccgaatttg aaaatattgt acggtgtgga agccaatata ttggattata aaggaactac
    15601 agacatcaac agcgaaatat tgaagcactg tgatataatt ttatgcgggt tccacttggc
    15661 agctttattt tcatcaccaa gtgacttctg gaactttata gtaaaaaatc aattagcaaa
    15721 aaacaataaa aagttttacg atgaaatggt tgaaaagaat actcaggcaa ttataaatgc
    15781 catgaataaa tataaaatta acattttaac ccatccggga gataaaatac cggttaacat
    15841 tgataaaatt gcacaagcag cagaaaaaac tgatactatg cttgaaataa acaacagtca
    15901 cgggcactta aacaaggatg aaattataag agcatcaaaa tacaatgtac aatttgtaat
    15961 aaacagcgat tcccacatta aggacaatgt ggggcgctgc gacaatgcac tgagagctgt
    16021 agaaggctcg ggtattgatt taaaccgaat agttaattta aaaagttaaa gggggagcat
    16081 atggagttag taattattac aggtatgtca ggtgccggga aaagccaggc aattaaggtt
    16141 cttgaagata ttaattatta ttgcatggat aatcttcctc ctgcactcct tccgaatttt
    16201 gccgagttgt gcaagagttc atcgatggac gttaacaaag tggcagttgt tgcggatata
    16261 agaggaggaa tcttttttaa agatttgttc aatagtttaa aagaactgaa aaataaggga
    16321 attgaataca gaatactttt tcttgatgcc tctgatgatg acttaatcaa aaggtttaag
    16381 gagcagagaa gacctcatcc tttgtcttca gacggtacga taattgaagg aatcctggaa
    16441 gaaagaaaca gattggaaga agtaaaaaag aagtctgatt atataataga tacaaccagc
    16501 atgacgcttg gaaggcttaa ggaagaaatt ttaaatattt ttgtgaaagg cagcatatcc
    16561 cataatttaa acataacaat agtttcattc ggctataaat acggtcttcc taaggaatcg
    16621 gacctggttt ttgatgtaag gtttctgcca aatccatttt atatagatga attaaaaaac
    16681 tcaaccggca atgatgaaaa tgttaaagat tatgtaatga gttttgaaac gacatccata
    16741 tttttggaaa agttaatgga cattcttaaa tttcttctgc ctttgtatat aaaggaagga
    16801 aaatcaagtc ttgtaatatc aatagggtgt accggaggca agcatcgctc tgttacaata
    16861 tcaaattatt tagcttccat gcttacggct gaaaattaca gagttttatt aaatcacaga
    16921 gatgcgggga aaaattaaat gaaaatagta gtattcggcg gagggacagg cctttctgta
    16981 ttgctcagag gattaaaaaa ttacacaaaa gacatatcag caattgtaac cgttgcagat
    17041 aacggcgggg gttccggagt tttaagagaa gacttaggca tgctgccgcc cggagatatg
    17101 agaaactgca ttattgctct ttcagatatt gagcccatta tgataaaatt gatgcagcac
    17161 cgtttcagcg acggatattt aaaggggcaa agcttcggga atttatttat agctgcaatg
    17221 tatgaaatat tcggcaatta cgaacatgct ttaaaggaaa taggaagtat tttcaggctt
    17281 tcgggtaaag ttcttcccat gaccctgcag gatgcacaat taaaagcgta tctcgctaac
    17341 ggcgactgca tattgggaga aaaatatatt cctgaatatg tgagcaaatt aaacacgaaa
    17401 atagataaaa tcgaattggt gccttatgtg tgcaaacccc tggaagaaac ggttgaggat
    17461 ataaaagaag cagatcttat tgtattagga ccgggaagct tgtataccag tgtaattcca
    17521 aatattttag ttgaaggtat accgtctctt ttggctgaaa gtaatgcaac cgtattttat
    17581 gtatgcaatc ttatgacaca gccgggagaa acagataatt acaatgtttt agaccatgta
    17641 aatgcaataa taaaacacag catgaaatac atgattgatt atgtaattgt caatgatgaa
    17701 aaaatatccg aagagttgat tttaaattat aaataccagg gtgcaaaaca ggtacttatt
    17761 gatgaaaagc agataaaaga acttgaagaa atgaatataa aggttatatg cagcaatttt
    17821 gtcgatataa gaaaaaacta tatcagacat aattcagata aaataggaca gctcttgtta
    17881 aactatatta atgaaaaaaa ataaaagggg gtgcttctat ggagaaatat aagagtatag
    17941 atccaaatca gctaagcata gcggatggta tgattgattg ggtaagagta gtcagcaaag
    18001 acaacatggt tttgtatgcc aacaaaaaaa tgcaggaaga tttgggagaa aatattgtcg
    18061 ggaaaaaatg ctatgacgtt ttatgcagat ataatagatg cgaaaactgt atttcaagca
    18121 caaccttgga aaagggctcc ataatgcgca aacatcataa attgggaaac aaaacatata
    18181 tgattatatc ctcaccctta tttgatgata agggtgaaat tgaagcttcg gtagaggtat
    18241 tcagggatat aacgaatgaa gttttacttt cggaaaagtt acaagaaaaa aacaaaaaaa
    18301 tgagtgatga tataaagttt gcaaaaaata tgcaaaatcg catgcttcct ccaaaaggaa
    18361 tgtataacgg cttgagcatt gactatttgt atgaatcatc agaatcatta agcggagatt
    18421 attttgatgt atttgaaata gatgaaaata atacaggtat atatatttgt gatgttgtgg
    18481 ggcacggagt aacggcatct ctcttgacta tttttgtgag gcaatcatta aggactattg
    18541 ccaaaggaaa tattaatttg aataaaatta tgaaggagct gcataaaacc tttttatcgt
    18601 tgaatttgga ctatgataaa tatttttcag tattttttgg aatatataat aaagattccc
    18661 atgtatttaa atatgtcaat gcaggacata actcggtacc gattttaata agcggtaaag
    18721 atattaaaaa gcttatgctt aagggatatc ctattgcaaa tatttttgat aatgttgatt
    18781 acattgtaga aaaagttaca ttaaaagtcg gtgataaaat actcctttac actgacggca
    18841 taacggagac aacaaatagc gaaggggtgc aattcggcgt cgatagattg ataaatacaa
    18901 taatatccga tggaaatata ttagaaaaca taaagtcatc tgttaataat ttcagcatag
    18961 ttcataaaga tgattatgca gtcttattag ccgaaataat ataaacattc tgagcgatag
    19021 cgaaatattt catgagatta ttcactgcgt tcagaatgac agggtaacag tagctattgt
    19081 atggaaatag ggaaggacaa ttgggagggg agaaaatgaa aaatattact cttgatttac
    19141 gaggaagctt tattgatgag gatgaattaa aaaaaattaa accggaaatc atggcagctc
    19201 aggagacgct tctggacaga aaaggagtca gcagcgaaat gacaggctgg ctcgattata
    19261 tgtacagaat tgacaaaaat gaatacgaag aaattaaagc tgcagcaaaa tatattaaag
    19321 aaaactctga agctttcata cttttgggaa taggaggctc ctatttaggg gcaaaagctg
    19381 caatcgaagc tataagggga gattttcaca atgaattgtt aagtcctaag gtatattacg
    19441 caggacaaaa tttaagtgga gcatatttga aaaaacttat tgagcttgtt aaagaaaaag
    19501 aagtttgcat tattgtaatt tcaaagtcgg gaacaacctt ggaaacagca ttgtcattca
    19561 gagtctttaa ggagctgata gagaaaaaat acggcgaaga atcaaaaaac cgcattttcg
    19621 tcgtaacaga taaatataaa ggtgccttga agagtatatc tgatttaaaa ggttacaaaa
    19681 catttgtggt tcccgatgat ataggaggaa gatattccgt aataactcct gtaggacttc
    19741 ttccaatggc ggctgcaggt cttgatacgg ataaattcat ggaaggctta atatcaggtg
    19801 cggaagaata ctcggtcaga gattttgaat gcaacattag cttactttat acagccgcaa
    19861 gaaacatttt aaacagacga aataaagaaa ttgaaatatt tgtaaactat gagccatcct
    19921 gcatatcaat tgccgaatgg tggaagcagc tttacggtga aagtgaagga aaagaaggga
    19981 agggtatttt ccctgcttcg cttaatttca ctacagacct tcattccatg ggacaatttg
    20041 ttcaagaggg aagagaaata attattgaaa caaccctatt aattgaaaat aatgaagatg
    20101 atatggtaat tccttttgat ttaaatgatt ttgataaatt aaattatata tcgggaaaaa
    20161 catttaactt tgtaaactta aaagcatttg agggaacctt catggctcac aatgagggaa
    20221 aagtaccggg gattattatc agtgttccta agatggatga attttatctt gccaaacttt
    20281 tttacttttt tatgatgagc tgtggaataa gtgggtatac taataaggta gaccccttta
    20341 ctcagccggg agtggaagca tacaaaaaga atatgttcaa gcttctaaag gaagaggaac
    20401 acaccaagtg agaggagaaa ttatgataaa actgggagaa atgcaggaat tagaagttgc
    20461 aaaaatagta aacataggtg cctatttgca aagtgaagac aaggagaaaa ctgaagacag
    20521 agtattgctt ccaataaagc aagttcctgc aggaacaaaa gtaggagata aaataaatgt
    20581 atttgtttac agagactctg atgacaaaat tatagccact gtaaaaaaac caaaaataac
    20641 attgggtgaa attgcaacct tgaaggttgt cgagatgtca aggataggag catttcttaa
    20701 ctggggtttg gaaaaagact tattccttcc ctttaaggaa cagataggcg atataaggct
    20761 aaatggagaa tacatggtcg gtctttatat tgacaagtcg gacagacttt gtgcaaccat
    20821 gaatttattt aaggtattga gaacagactc accttacaag gttaacgaca ttgtaaaggg
    20881 tactgtattc agcttaaaaa gagggctcgg tgcaatggtt gcggtagaca acaagtattt
    20941 aggattaata catgagggag aaataataaa gcctcttcat cccggacagt cagttgaagt
    21001 gagggtcagc aatataaagg aagacgggaa actggattta agcctaaagg atgctccgag
    21061 gcttcaaata gacaaggacg gtgaaaaaat cctaaaggta cttattagaa acaagggctc
    21121 cttaccttta aacgatgaca gcagtcctga agaagttaat aagattttgg ggatgagcaa
    21181 aagctccttt aaaaaggctg caggacgttt aatgaaaaga agattgatta ttatgaccaa
    21241 aaacggcata gaaatgcttt ctgatgatga aaaacaacaa tccaaggtaa aaaagtccaa
    21301 tataaggtac aaaaagaagt agcttagttg acaaaagatt attagctgat aacctatata
    21361 aaaaacacaa ggaggaatta tatgtctaca ccacacaata gtgcagttat agggcagatt
    21421 gccgaaacag tcttgttgcc cggcgacccc ttgagggcaa aatttattgc agaaaacttt
    21481 cttgaagatg ttgagcaatt taacactgta agaaatatgt atggttttac cggaagctac
    21541 aaaggcaaaa aagtttctgt tatgggaagc ggaatgggct gtgcttcaat aggaatttat
    21601 tcttatgaat taatacatat gtacggagtc aaaaatttaa tacgcgtagg ctcctgcggg
    21661 gcttatgcac ctgatttgaa tttgtacgac atagtacttg caataggagc aagcacagat
    21721 tcaaattacg cttatcaata tgagctgccg ggaatttatt cggcaacagc ttcatgggag
    21781 cttttatcaa aggctaagaa ggttgcggat gataacaata taaaagcagt agtggggaat
    21841 attgttacta ctgatatttt ttatcatgat aattctgaga attggagaaa atgggcaaag
    21901 atgggctgtt tagccgtcga aatggaaacc tacgccttgt attgcaatgc caacagggca
    21961 ggagtaaatg cccttacaat attgacagta tctgattccc tcgagcataa gactgaaact
    22021 accccggagc aaagagaaaa gagctttacg gacatgataa aaatagcatt ggagatatta
    22081 taacatataa tgtgcagcga gcctgcgagc cggaaaaaaa agcacctatt cttttatagg
    22141 tgctttagag tatacattag atgatttcta cgttagcagc ttgagggcct ctcgttcctt
    22201 caaccacttc aaaggttact ttttgtcctt cttccaaagt cttgaatcca tctgcgttta
    22261 tagcggaaaa atgaacaaac acatcattcc cttcatcgct tgtgataaag ccaaaacctt
    22321 tcgcagaatc aaaccatttt actgtacctg ttttcattaa aactcctcca tgttttttaa
    22381 aaataaataa tataatgaaa aagaactaca catgtagttc ttatcacgtc tccttcaaat
    22441 actatattaa tataagtaat gcatttatct atattaatgt attatttcga aaaacaatta
    22501 ctactatgct acagtaacat tgctagcctg gggtcctttt tgaccatcaa ctatgtcaaa
    22561 gttaactttt tgtccttctt ccaaagtctt gaatccatcc gagtttatag cggaaaaatg
    22621 tacaaaaaca tcctttccat catcacagga tataaatcca aaaccctttt tggaatcgaa
    22681 ccattttact gtacctgttt tcatttaaaa aaatcctccg atattaaaaa agatatcatt
    22741 attgtaacag ttttttttgt aaattgcaac atatttattt cttaaataaa aaaatcatcc
    22801 ttaatgtttg ttataaagtt ggtgataatt gtgcgtggca aaataatttt tacaatccaa
    22861 ttgaaaaaga aacggtactt tgttataatt atttagcgat gattttataa aggtgggaca
    22921 tgattaaaca ttttttaaag gtattttttg cgtctttttt acttattgcc ataatattga
    22981 gtgttactgc attagggtat atttttctgg tcgataagga tattgtgata gggaaaatca
    23041 aatttgtaaa tgatgagaag agcgaggttt ctttctatga atcgagttcc tttgattatg
    23101 gaacttcgat aggaaaagct gcgtctaata tgaaaagatt caatattctt ttggcaggac
    23161 tggaaaatca aagaacagat acaataatag ctctaagtta cgatatggag agtaaaacag
    23221 ctgacttgat ttcattgcca agagatacat atcttccaag aaatgcagat gataatgccg
    23281 aattaaaaaa aattaatgca atatattctc atgaagggat tgaaggactt gcgttaagag
    23341 ttcaggaact gttgggaatt ccgattgaaa agtatgtggt tattgattat gagggagtaa
    23401 ttgcatgcgt tgatatgatt ggaggagttg aggtgaatgt cccctttcat atggaatata
    23461 ccgaccccta cgatgatcct cccttatata tagatattcc tgaaggtgtg cagcttctta
    23521 acggggagca gtctttaaaa tttttaagat acagaaaggg atatgacaac caggatttgg
    23581 ggagaataga agcacaacag caatttatta aatctgccgt taaaaaaact ttgggttttc
    23641 aactgcccgc tgtgataaaa gaagcttatt ctcacattga aacaaacatt a
//
LOCUS       DAOSHP010000018        23687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1696_length_23687_cov_8.798282, whole genome shotgun sequence.
ACCESSION   DAOSHP010000018 DAOSHP010000000
VERSION     DAOSHP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23687
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1696_length_23687_cov_8.798282"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1875
                     /locus_tag="PLB33_03225"
     CDS             82..1875
                     /locus_tag="PLB33_03225"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOG62563.1"
                     /translation="MSRIKNFMNNIVYNLKETITRFPMTIVFLASLSTVMFLIIEDYS
                     SLDIDLLSRYMFAGIFGAFLATTVRFMMERYENLKNSFLFYGLTILLTIGYFYFMTDD
                     KVDNKMLIHLLVISFALFAAYLYLPSSKNALNFGNVALAHFKSAFTAILYGIVLYVGI
                     AAIIGAIDILLYDVDYKSYAHAANIIFIFFTPLYYLSLLPKFNSRDENDDAKKEISFT
                     YPRFLDILVSYITIPLITAFTAVLIIYFIKILATGVWPVGQVGPMVLGYSAAGYFIYI
                     LSSNLNNKFSVLYRKLFPLILIPLVVMQMVSSYIRIDAYGITESRYYVVLFGIFSIVC
                     ALVLIFGKKKNPNTIVLLSAVFALMSIIPPVDAFTVSKNSQERRLTEILNRNKMIVDG
                     EIVKKTDLSTDDMFEITNISNYMYGMGYLDDMDWFPDKYADNYYGNFKNIYGFEQYYD
                     RGFPGQEETLYLYAILDENEKINIEDYDLFFRIYIQNKRGPGEDIGEFSLKGKNYSIK
                     QIPDEKGYITITVSDDTNTLIEIPMKEFIDGLFEKSNAPKGAMDKESLTIEKQNDKLK
                     VKLLIYDMNVDRNDPDDIYISANIYVFVSTQ"
     gene            complement(2044..3459)
                     /locus_tag="PLB33_03230"
     CDS             complement(2044..3459)
                     /locus_tag="PLB33_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG62564.1"
                     /translation="MGPIDLIIFALYMIGMVVIGFIAQGKIETMDDFILGGRRFGLVA
                     LVGTIMATMIGSGMTMGAVGNAYKAGAGGTVFWMYTGFSIGLLYFGYIAGKIRETGKR
                     TVAEVLSAKFGNNTRLISSIVIIGYAVSIVAINIAGLRNVIFYVFGSKLGISLPLATT
                     IAAAICILYTSLGGFYAVVWTDVVQFGLMLLGIVILGPIIGLNSAGGFNNVVLEYEKI
                     GSSITNPFINGISSGSIGFFLAYFLTVPGDPTMPQRALAAKDEKSAKISFYISGVLGF
                     FFGFALLIMGGSAFVLMPGLENPESAMLLFISEYYPPILKGLSISAIVAAIMSSFDSF
                     LILATTHLVYDVGSSLNKDVNEEKMKKLLPKVTIVIGILGLIIALFIQSLFDYLYMVF
                     SIIGSSLVPAIIAALYYSDKTTSKAVVASIVTGAVVPGALYLTYGYDVFLGDPVFLGI
                     IASTLVLIIGSIVTKDKIATK"
     gene            complement(3504..4433)
                     /locus_tag="PLB33_03235"
     CDS             complement(3504..4433)
                     /locus_tag="PLB33_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG62565.1"
                     /translation="MNICIIDAKQFITDEKLIEPLYKLGNVSMYDGIPQSLDETIKRA
                     KDADIVLFALMKFTNEIIDNLPNLKILQFIGTGVWNFVDALYAEKKGIKVLNIEGYGN
                     NAVAEFALSCAFSLARKITNADRILKERRWSLDNLEGIEIEGSVFGVVGTGNIGSIVA
                     KKACLLGADVIACDIYESDELKNKYGIKYVSLSEIFEKSDIITIHMKATKETEKIIGK
                     DLLSKMKKGSLFINVARAELVDNESLYELIKGGHIAGAAIDVYENEPPKDYKMAELQN
                     VIATPHIGFYTKKANDNSIRLSVESVLNFLDID"
     gene            complement(4459..5625)
                     /locus_tag="PLB33_03240"
     CDS             complement(4459..5625)
                     /locus_tag="PLB33_03240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG62566.1"
                     /translation="MKHKFIAKRYWNTKSSAMGEIGPLMSKYNDVIDLTFGDPDFTTD
                     EIIIKEAFKDALNGHTHYTSFYGDDELRDEICKYYHDEFNIKVERNETFISTSAAQGL
                     WLALETITDDGDEIIIHEPYFTPYPDQIKLTRGIPVFLETFEEEGFQINVERLEKLIT
                     NRTKAIIINSPNNPSGARFSDETLLKIADIAEKYDLIVIVDDIYGLYTFDSENRNTPF
                     MSIKNMKERTITICSASKDYAMTGWRIGYLIAPDYLVDAMEKVNENNVFTAPSVSQRA
                     MLHAIRNRKIIQPKLFNIFKERVFYTYDRICQTKNMSVLPPRGTFYLFPNIKKTGLSS
                     QEVSMKILDEAHVLTIPGSGFGSSGEGYIRIACTVGIDKLGEAFDRINKMDIFR"
     gene            complement(5736..6506)
                     /locus_tag="PLB33_03245"
     CDS             complement(5736..6506)
                     /locus_tag="PLB33_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOG62567.1"
                     /translation="MFRNVIVRRPSKSMVEGITSSPELGKPDYELALKQHDAYIEALK
                     ACGVKVTVLEALEEYPDSCFVEDTAVLTPKCAIITNPGALSRRGESALMVDTIKKFYN
                     EDQIEYITSPGTIEGGDVMMVGNHFYVGLSARTNEEGFRQFKAALEKYGHTSSLVSLE
                     KVLHLKTGVNYIENNIMLISGEFIEKEEFKSFNKIIVPENEAYGANCIWVNDTVIVPE
                     GYPTVEKGVRDTGYKVIVVDTSEYRKLDGGLSCLSLRF"
     gene            6795..7451
                     /locus_tag="PLB33_03250"
     CDS             6795..7451
                     /locus_tag="PLB33_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG62568.1"
                     /translation="MSQYLTLKDHVYNYISSKINDGSLKPQDKINEQQISDELEISRT
                     PIREALIQLASEGYLENRARKGFRVRSLDYEKTKELYEIIGLLDGRIAYMTVDLINDN
                     HLKNMRFLADSMASAIEQGLNQKYYELQIEFHNIYTDLYNNQEMINLLNKLKKNFMRK
                     YYFFETKTDEKAILKKTNDQHYEIIRLFEEKKKEELERYIRDIHWAVDIAIYDSLQNY
                     "
     gene            complement(7518..8399)
                     /locus_tag="PLB33_03255"
     CDS             complement(7518..8399)
                     /locus_tag="PLB33_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOG62569.1"
                     /translation="MEVGSRSMVDKIQTIVIKHAREAYVSQEYLNENWQKWGFFSCPD
                     YNKCVEEYDAFEALIKKLVPDVRLLPKDEESGLDSIYTHDSVKFTEKGAIILNMGKNA
                     RKNEPKAMKVYLESIGVPILGEITGEGTVEGGDVLWLEDNRVAIGRGYRTNDEGIRQF
                     KELVKDLVDEVIVVPMPHGNNEEECLHLMSVISPIDKDLAVVYSRYMPVFFRQMLIKK
                     GIKLIEVIDDEYMPLGCNVLTIEPRKVVLLKGNPDIKKKLLNEGVEVHEYEGQHISLP
                     GNGGATCMTAPLLRIKN"
     gene            complement(8615..9367)
                     /locus_tag="PLB33_03260"
     CDS             complement(8615..9367)
                     /locus_tag="PLB33_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOG62570.1"
                     /translation="MNEFSRTQLLLGKQAMEKLSSSTVAVFGIGGVGTFVVEALARCG
                     VGNFALFDDDKVCITNINRQLIATRKTIGRKKVEVMAERIKEINPKASTATYQVFYMP
                     DNADEYDLTSYDYIVDAVDTVTAKIELIVRAKKNNIPIISSMGAGNKLNPTAFEVADI
                     YKTSVDPLAKVLRHELKRRGIENLKVVYSKEKPLTPSCDEDSCKDSCVCPPGTTRKCT
                     QRRAIPGSVSFVPSAAGLIIASEVVKDIIAGI"
     gene            complement(9546..10013)
                     /locus_tag="PLB33_03265"
     CDS             complement(9546..10013)
                     /locus_tag="PLB33_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOG62571.1"
                     /translation="MSVEKVREYLKQFNKENDIKEMDESTATVELAALALDTEEARIA
                     KSLSFYNGDGAMVIVVAGDAKIDNRKFKDYFGFKARMLSPEDTKKFTGHEVGGVCPFA
                     LPDNVKVYLDVSMKRFETMFPACGSGNSAIELTLDELEETSKSNTWIDVCKNW"
     gene            complement(10070..10837)
                     /gene="pflA"
                     /locus_tag="PLB33_03270"
     CDS             complement(10070..10837)
                     /gene="pflA"
                     /locus_tag="PLB33_03270"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOG62572.1"
                     /translation="MTAGFLHSIETLGTLDGPGLRMIVFLQGCKLRCLYCHNPDTWIE
                     NITPGDNKNLTDKYYLKERFTADYIAEKAKRYKPYFKNNGGITFSGGEPLRQPKFLLD
                     CLMLCKAEGIHTVIDTSGVGFGDYDEILKYTDLAILDIKHSNPEKYKKITGCDIEYYL
                     DFKEAVIRNKIQLWIKHVVTPGINDTEGDMKDFENEVKTFPDKLIQKIELLPYHTLGV
                     YKYKELNIKYKLEGVPPLSKDRLQELKDCITLEKLIR"
     gene            complement(10840..13065)
                     /gene="pflB"
                     /locus_tag="PLB33_03275"
     CDS             complement(10840..13065)
                     /gene="pflB"
                     /locus_tag="PLB33_03275"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOG62573.1"
                     /translation="MKEWNNFIQGRWTKEIDLRDFIQKNYSPYEGNEEFLSGATEKTK
                     NLLKKVSRLMTEELKKDVLDVDTDNISGINNFEPGYLDKDNEIIVGFQTDAPLKRMIN
                     PYGGIRMVEQSLEAYGYSMSDELCNVFHKYRKTHNEGVFDVYTPEILLARSSGLLTGL
                     PDAYGRGRIIGDYRRIALYGIDFLVEKKKEEYNNIKFPMNEDSIRAREEIKEQIKALF
                     DMKSMASKYGIDISKPSSNALEAVQAVYFGYLAGVKENNGAAMSLGRTSTFIDIYIER
                     DIKEGILSEKEAQEIIDQFVLKLRMVRHLRTPEYNELFAGDPNWVTEALGGMGLDGRT
                     LVTKTSFRFLHTLTNLSAAPEPNMTVLWSERLPEGFKKYCTKMSIDTSAVQYENDDIM
                     RPIYGDDYGIACCVSAMAIGKEMQFFGARCNLAKALLLGINGGTDEISKETVITNINK
                     INSKILNYDEVLNNYKKVLEYVAELYVNTMNIIHYMHDKYAYESGLMALHNYDVRRLM
                     AFGVAGLSIAADSLSAIKYAKVKPVYGEDGITEDFEIEGEYPMYGNDDDRVDGIAAEL
                     LEFFSNELKKHKTYRNAKHTLSVLTITSNVVYGKKTGATPDGRKKGEPFAPGANPMNG
                     RDKSGLLASLNSVAKLKYKDICEDGISNTFTVVPDTLGKKYRHRIDNLSNILDGYFSQ
                     GAHHINVNVINRDTLYEAMEAPHKYPNLTIRVSGYAVNFNRLTREQQMDVIGRTFHDR
                     M"
     regulatory      complement(13121..13203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(13246..14127)
                     /locus_tag="PLB33_03280"
     CDS             complement(13246..14127)
                     /locus_tag="PLB33_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62574.1"
                     /translation="MDDIIIEDNLEDEVESWEPNFWERLFSNKMAVFGATVILILFIT
                     AIFAPLIAPYPYDAIDLPQMLKAPNSENILGTDEFGRDIFSRIVYGSRISLQVGFIAV
                     GISLVIGTILGALAGYYGGITDYIISGITDIAYSFPISLLAIAFVAALGRSLTNLIFA
                     VALVSWSGYARLVRGQFISLKNREFIEATKILGMSDARIIFKHMLPNSMAPVIVLTTL
                     EIPKAIIVESSLSFLGLGVPPPAPSWGSIMSSGRAYIMEAPWVSIFPGLVMALAVLGF
                     NLFGDALRDTLDPRLKD"
     gene            complement(14169..15086)
                     /locus_tag="PLB33_03285"
     CDS             complement(14169..15086)
                     /locus_tag="PLB33_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62575.1"
                     /translation="MLKYIFKKLLMMIPILLGLTIILFLILHLAPGDPVHLVVGPNVT
                     PEVYENVRRSMGLDRPLIIQYFDYLSNLVKGDLGSSILQHRPVADIVLERFPITIGLG
                     VRALLLSFLIAVPVGIIAAVNRNKSADFIAMTFALVGISMPTFWFGIILLYFFAYKLG
                     LFPISGYGTWKHLVLPVITMGLTDAAVIARMMRSSMLEVIGQDYIRTARAKGLREKVV
                     TNKHALKNALIPVITLLGMRMGWIIGGSVTLEIIFSIPGLGRTMVDSIFSRDYPVVQG
                     SVLILATSVMISNLIADILYALVDPRIRY"
     gene            complement(15147..16748)
                     /locus_tag="PLB33_03290"
     CDS             complement(15147..16748)
                     /locus_tag="PLB33_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG62576.1"
                     /translation="MKKNISKMFALVLILSCTALVFSGCSKNDVPVGETPEGNNGEQP
                     AKETTLVIAYERDAETLNHIKTGWYSDSLIYMYDRLVSRDYDFNYRPSLAISWETSED
                     GMVWTFHLRDDVKFHNGKPLTAHDVKFTFDTILDPETASPSQSDFAAIKGLEVKDEHT
                     VDIMLHQPFPNLLFVLSNTAAGIVNKEAYEEYGDEYGINYVVGTGPYMFKEWKQGDKI
                     IIVKNPDYNWGPEWMTNRGPALIDRIEFRTIPEENTRLMELEAGNVHILRDVPASFLQ
                     RVEEIDDVEVMRRDATKLGYLAYACDKEPFTDIRVRRAINHAINKEDIIKYVFRGYGE
                     PAYGYLPPALKSEYYADSKKDGYEYDVEKAKQLLKEAGYGDGLKLTLSAENSTEYSRL
                     AEVLQDQLKQVGIETEIQLYDSSSYTSMLREGQQELFIRLYSWPNADILDWFLLSSQF
                     PYPNHSRWQDEKTDELINKAATSPTWDERSKGYFEVQKHLIEQAVWAPIYIPENTIAV
                     RKEVKDFKFHPWIPQYNDGTYLEVE"
     gene            complement(16753..17736)
                     /locus_tag="PLB33_03295"
     CDS             complement(16753..17736)
                     /locus_tag="PLB33_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG62577.1"
                     /translation="MSGNILEVKNLKVHFPVKGELIPKNVGYVKAVDGISFNIGYAET
                     LGLVGESGCGKSTTGRAVLNMLKVDSGQVFFEGKDILKSSRKELKKIRRDMQLIFQDP
                     FGSLNPRMTIGSALEEVLYIHHIRDKNERQKRVIELIEMVGLNKNYLSRYPHEFSGGQ
                     RQRAVIARALAVKPKFIVCDEPVSALDVSIQSQIINLLEELQKNFKLTYLFIAHDLSV
                     VKHISNRIAVMYLGRIVEIAESDELFKRPAHPYTKALLSSIPVCDPDFRKNIILLEGD
                     LPSPANPPKGCRFHTRCIYAREDCSRKEPVSVNLGNGHMVECHLYSNEEED"
     gene            complement(17733..18716)
                     /locus_tag="PLB33_03300"
     CDS             complement(17733..18716)
                     /locus_tag="PLB33_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62578.1"
                     /translation="MSEYVLEIRDLSICFYSGRKEIPAVENLSFNLSRGETLGIVGES
                     GSGKTVTSLSIMKLIPNPPGKITGGEMFFGSLDLLKVDEDTIRQIRGNNISMIFQEPM
                     TSLNPVYTIGEQLSEVFCTHQKLSKKEAMKKSLDMLKQVKIPLAEKRMKQYPHELSGG
                     MRQRVMIAMALACMPEILIADEPTTALDVTIQSQILHLIREMKEKMNTAVILITHNMG
                     VVAEMADNVMVMYCGRALEYADVRTLFKEPKHPYTIGLLNSIPRIKGGRKKLYAIKGS
                     VPVPGEITAGCRFNSRCEYVFEKCLVEEPPIFEVGKSKVRCWKYEGKGVTV"
     gene            complement(18973..19878)
                     /locus_tag="PLB33_03305"
     CDS             complement(18973..19878)
                     /locus_tag="PLB33_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62579.1"
                     /translation="MHNKYNEKISDMKKCIQKKNKLNLLLKQTEQDIIKEKLLLNKLS
                     GELEKEYQDALKLKSDNIISLFYEILGTEEDKHNKEKQKLLKARLKYEQCKNNMNYLV
                     NETKKIVDCIEKLNGCDAEYEELINKKIETINIEDNETGQALKKLIKRKENMNANIIE
                     ICEAICYGEKALEAIEMTIKELEAAENFENSDGSNNIENARGYAEQTHRMLGRFKKEL
                     SDITIVTGTEIAVGTFENFADCFFDNLIFDWVVQSGIRKSLDTVKNTKNQLDKAMSKL
                     YEEKVTEEFMIKQIEEQINQIIEKV"
     gene            20116..20298
                     /locus_tag="PLB33_03310"
     CDS             20116..20298
                     /locus_tag="PLB33_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOG62580.1"
                     /translation="MITKDMTIREVIMKNQEAAEILMTFGMGCVGCPSAQMETLEEAA
                     AVHGMNLDDLLAALNK"
     gene            complement(20510..20734)
                     /locus_tag="PLB33_03315"
     CDS             complement(20510..20734)
                     /locus_tag="PLB33_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOG62581.1"
                     /translation="MKKIKIYTGSSCSNCTAAKEYIKEKGYEYEEKNISADSEAKKEL
                     IALGYMGVPVIMIDDEVVVGFNKSKLDEIL"
     gene            20888..>21687
                     /locus_tag="PLB33_03320"
     CDS             20888..>21687
                     /locus_tag="PLB33_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62582.1"
                     /translation="MKECTIYIDVPEKFLKGAGIPNSRFIPSYIYQIKIILKNNSSQK
                     KQCSFSATMGNGIRYLENIKHEGPDINLLDTVSAVEPCKELANDNVIIFGNNLTLSPN
                     STNTIIFDGALCDKYTINSIENSGDKIPHRSKINFYAHLLDEDKIDDSYFISSTAMDS
                     DMIVKCDDEKLNAGDITKYYIDICTGQYDLARKVYLRSILDAGLEYIGDSCNLEPRNI
                     YEFNGKTIIKWDIGSLQPSEIKRIGYKVKVKNDAKGKLTNKLNSNCIN"
     assembly_gap    21688..21787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21946..>23687)
                     /locus_tag="PLB33_03325"
     CDS             complement(21946..>23687)
                     /locus_tag="PLB33_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOG62583.1"
                     /translation="GTQLIYYRIFYTFTSFYSIFVGTAKALSFIDIMTDTLRNNIAEL
                     FAIFLPLGVLVYYHRKLQFDKIHKNYIIKVAVSAVIMQAAVVITVLSSDMGMLSPSYL
                     YSETFLVVESVDKFGLLTTGRLDIKNILKSIVFAVEPEHSEIIESEEPDPADELPGEP
                     QSEEPTIEVAEYNTMDIPFEELIKKEGNEAIRSMHKYFSSVKPTKKNEYTGKFKGKNL
                     ILITAEGFSPYAVRKDITPTLYKMQEEGFKFNNFYTPTWGVSTSDGEYVACTGLLPKE
                     GIWSFYKSSKNYMPFAMGNQLKKEGYETKAYHNHYFDYYYRHKSHPNLGYTYKGLGNG
                     LNVTETWPESDLEMIELTVPEYIDSQPFHVYYMTVSGHLQYTFTGNYIAAKNRSLVEN
                     LPYSKNVKAYLACNIELDKAMEKLIQELEKKGIAEDTLIAISPDHYPYGLTLNEISEL
                     AGKEIKSDVDLDRGIFILWSKGMEPVKIDKVCSSLDIIPTLSNLMGLEYDSRLLMGRD
                     IFSDSEPLVMFSDRSFITDKAFYNARTNEVTSFTDENIESSYIKETAETVINKFKYSS
                     MILDTDYYKIILE"
BASE COUNT         7197 a         4703 c         3531 g         8156 t
ORIGIN      
        1 tgtggtatat attactttta taagtaaagg aacaatattt tataaaaatt cgtcataaat
       61 tatatagaaa gaggtgatag aatgagcagg attaaaaact ttatgaataa tattgtttac
      121 aacctgaaag aaacaattac aagatttcct atgactatag ttttcttagc ttcattgtct
      181 acggttatgt ttttgataat tgaagattat tcatcgttgg atatagattt attatcgaga
      241 tatatgtttg caggtatttt tggtgcgttt ttggcaacaa cggttcgatt tatgatggaa
      301 agatatgaga acttaaaaaa ttcattttta ttttacggat taaccatttt acttacaatt
      361 ggttatttct atttcatgac ggatgataag gttgacaaca aaatgcttat acacttgctg
      421 gtaatttcct ttgcattatt tgcagcatat ctgtatttgc cgtcatcaaa aaatgcttta
      481 aattttggca atgtggcact tgcgcatttc aaatcagcct ttacggctat tctctacggt
      541 atagttttat acgttggtat tgcagcaata ataggtgcta tcgatatatt gctttatgac
      601 gtagattaca aatcttacgc gcatgcagcc aatattatat ttattttctt tactccttta
      661 tattatcttt ccttattgcc gaagtttaat tcaagggatg aaaatgatga tgcaaaaaag
      721 gaaatttcat ttacctaccc aagatttttg gatatattgg tttcatatat aacaattccg
      781 cttattaccg catttacggc agtcttgatt atatacttca ttaaaatact tgcaaccgga
      841 gtatggcctg ttggacaggt cggacctatg gttttgggat attctgcagc aggttatttc
      901 atttacattt tgagctcaaa tttgaataac aaattttctg tgctttacag aaaactgttt
      961 ccattaattc taataccctt agttgtaatg cagatggttt cttcatatat tcgtattgat
     1021 gcatacggca ttacagaatc cagatattat gtggtgttat tcggaatatt ttcaatagta
     1081 tgtgcattag tgctcatatt cggtaaaaag aaaaatccaa atactattgt tctcttgtct
     1141 gctgtctttg cactgatgtc aataattcct ccggttgatg cgtttaccgt ttctaaaaac
     1201 agccaggaaa gaagactcac tgaaattctt aacagaaaca agatgatagt agacggtgaa
     1261 attgttaaaa aaacagatct ttctactgat gatatgtttg aaataactaa tatttcaaat
     1321 tatatgtatg gaatgggata tttggacgac atggactggt tcccggataa atatgccgat
     1381 aattactatg gcaatttcaa aaacatctac gggtttgagc agtattatga cagaggattt
     1441 ccgggacagg aagagacact ttatttatat gccatacttg atgaaaatga aaaaattaat
     1501 attgaagatt atgatttatt tttcagaatt tatattcaaa acaaaagagg acctggcgag
     1561 gatataggtg aattttctct gaaaggtaaa aattattcga ttaagcagat acctgacgaa
     1621 aagggttata taactataac cgtttcggat gataccaata cattgattga aataccgatg
     1681 aaggaattta tagatgggct ttttgaaaag tcaaatgctc ctaaaggggc aatggataag
     1741 gaaagcctta caattgaaaa gcaaaacgat aagttgaaag ttaaattgct tatatacgat
     1801 atgaatgttg ataggaatga tcctgatgat atatatattt ctgcaaatat ttatgtgttt
     1861 gtatctactc aataacgaag gaactcaagg gatttttcga gctaagtact cataatgaca
     1921 gaaatgtcat tcaaagcaat aataaaacca gaataaactt tatcataaaa aactgaaggt
     1981 caagaactat aagttcttga cctgttggtt tttaccttta catcagctct aaaagagtgt
     2041 caattacttg gttgcaatct tatctttagt tacaatagag ccaattatca agactaaggt
     2101 agatgcaata attcctaaaa atacagggtc tcccaagaat acatcatacc cgtaagtcaa
     2161 gtagagtgca ccgggaacaa cagcgcctgt aacaatactg gcaacaactg ctttgcttgt
     2221 cgttttgtca ctgtaataaa gcgcagctat tattgcaggt accagggacg agccaattat
     2281 tgagaatacc atatacaaat agtcaaataa tgattgtata aacagagcta taattaaacc
     2341 aagaattcct attacaatag taacctttgg aagaagcttt ttcatttttt cttcatttac
     2401 atccttattt aatgaactgc ccacatcgta aacaagatgt gtagttgcaa gaattaagaa
     2461 ggaatcaaat gatgacataa ttgcagctac tattgctgaa attgacaatc cttttaaaat
     2521 aggaggataa tattctgata tgaataaaag cattgcagat tccggatttt ctaatcctgg
     2581 catcaacaca aatgcagaac cacccataat taataaagca aatccgaaaa agaaacctag
     2641 cacacctgaa atatagaatg atatttttgc agacttttca tcctttgcag ctaaagctct
     2701 ttgaggcatt gtcgggtctc cgggtacagt aagaaagtat gcgaggaaaa agccaattga
     2761 gcctgagctt ataccgttaa tgaatggatt tgtaattgat gaacctattt tttcatattc
     2821 taatactaca ttgttaaatc cccctgcgct attaagacct ataataggac ctaatataac
     2881 aatgcctaac aacattaatc caaactgaac tacatctgtc caaacaactg cataaaaccc
     2941 acctaatgat gtgtataaaa tacatatagc tgcggctatg gtggtggcta agggcaatga
     3001 aatcccaagc ttgctgccaa atacatagaa tataacattt cttaaaccgg ctatatttat
     3061 tgcaacaatt gataccgcat atcctataat tacgatagac gatattaatc ttgtattatt
     3121 gccaaattta gcagataata cttctgcaac ggttcttttg cctgtttctc ttattttgcc
     3181 tgcaatataa ccaaaataaa gcaaaccaat agaaaagcct gtatacatcc aaaaaactgt
     3241 tccaccggca ccggctttgt acgcatttcc taccgcaccc attgtcatac cggagcctat
     3301 catagttgcc ataatagtac caaccaaagc aactaaacca aatcttcgcc ctccaagaat
     3361 aaaatcatcc attgtttcaa ttttaccttg tgcaataaat cctattacaa ccatccctat
     3421 catataaagt gcaaaaataa ttaaatcaat aggtcccata ttaatcctcc tctaataata
     3481 tctttttata attattgatt tttttaatca atatctaaaa aattcaaaac tgattctact
     3541 gataatctta ttgagttatc gtttgccttt tttgtgtaaa aacctatgtg cggagttgca
     3601 ataacatttt gcagttctgc cattttataa tcttttggag gctcgttttc atatacatca
     3661 attgctgcgc cggctatatg tccacctttt atcaattcat aaagtgattc attatcgact
     3721 aattcagctc ttgctacatt tatgaataat gagccttttt tcattttaga caataaatct
     3781 ttcccgataa ttttctctgt ttctttcgtt gctttcatat gtattgttat aatatcagat
     3841 ttttcaaata tttcagataa actgacatat ttaataccat acttgttttt caattcatcc
     3901 gattcataaa tatcacaagc aataacatca gcacctaaca gacacgcctt tttagctact
     3961 atcgagccga tatttcccgt accgactaca ccaaacactg aaccttcaat ttcaatacct
     4021 tccagattat ccaaagacca ccttctttct tttaatattc tgtctgcatt tgtaattttt
     4081 ctggctaaag aaaatgcgca gcttaaagca aattctgcta cggcgttatt accgtaacct
     4141 tcaatgttta aaaccttaat gccttttttc tcagcataca acgcatcaac aaaattccaa
     4201 actcccgtac ctataaattg caaaatcttt aagtttggta gattatcaat tatttcattt
     4261 gtaaacttca taagcgcaaa taaaacgata tctgcatctt tagccctttt gattgtttca
     4321 tccaaacttt ggggtatgcc atcatacatt gaaacatttc ctaatttata caagggttct
     4381 ataagttttt catctgttat aaactgctta gcatctataa tacaaatatt cataacttca
     4441 tcctttcaaa caaaacaatt acctaaaaat atccatttta tttattctgt caaatgcttc
     4501 gcctaattta tcaataccaa ctgtacatgc tatccttata tacccttctc cgctacttcc
     4561 aaaaccactt ccaggtattg taagaacatg tgcttcatca agaatcttca tagaaacctc
     4621 ttgagaagat aaacctgtct ttttaatatt agggaataaa taaaatgtac ctcttggtgg
     4681 taagacactc atattttttg tttgacatat cctgtcatag gtataaaaaa ctctttcctt
     4741 aaaaatatta aaaagttttg gttgaataat ctttctattt ctaatagcat gtaacattgc
     4801 tctttgagaa acggatggcg ctgtaaatac attgttttca tttacttttt ccattgcatc
     4861 cactaaataa tccggagcta ttaaatatcc gatgcgccat ccggtcattg cataatcttt
     4921 cgaagcactg catatagtaa ttgttctttc tttcatattt ttgatactca taaatggtgt
     4981 gtttctattt tcactgtcaa aggtatataa tccataaata tcatcaacta ttacgataag
     5041 atcatatttt tcagctatgt ctgctatttt taataatgtt tcatcactaa accttgctcc
     5101 tgaaggattg ttgggtgaat ttattattat tgcttttgta cgatttgtaa ttaatttttc
     5161 caatctttca acattgattt gaaaaccctc ttcttcaaat gtttccaaaa aaaccggtat
     5221 gcctcttgtt agctttattt gatcgggata aggagtaaag tatggttcat gaataataat
     5281 ttcatcgcca tcatctgtta tggtctcaag tgcaagccat aaaccttggg cagcacttgt
     5341 tgagataaat gtttcatttc gttcaacttt tatattgaac tcatcatgat aatacttaca
     5401 tatttcatct cttaattcat cgtctccata aaaacttgta taatgtgtat gaccattaag
     5461 tgcatcctta aatgcttcct tgataataat ttcatccgta gtaaagtccg ggtctccaaa
     5521 tgtcaaatca ataacatcat tatatttgct cattaaagga cctatttctc ccattgcaga
     5581 tgatttcgta ttccaatatc ttttagctat aaatttatgt ttcatataaa cctcctttga
     5641 ataacattct attaacgctt tccttatccg tcttaatttc ctgcaatatt atctctcata
     5701 attttttagc atttgcgtat ataatgatgt atttgttaga atcttaatga taaacagctc
     5761 aatccaccgt ctaattttct atattctgac gtatctacta caataacttt atatcctgta
     5821 tctcttacac ctttttcaac agttgggtaa ccttccggca ctataactgt atcattaacc
     5881 caaatgcaat ttgcaccata agcttcattc tcaggaacta taattttatt aaatgattta
     5941 aattcttctt tttcaatgaa ttctccagaa attaacataa tgttattttc aatataattt
     6001 actcccgttt ttagatgcaa tactttttcg agagaaacta atgagcttgt atgtccgtat
     6061 ttttcaagag cagctttaaa ttgtctaaag ccttcttcat tagtacgtgc agatagtcca
     6121 acatagaaat gatttccaac catcataaca tctccaccct caattgttcc cggggatgtt
     6181 atatactcaa tttgatcttc attataaaat ttcttaatag tatcaaccat aagtgctgat
     6241 tctccccttc tggaaagtgc cccgggatta gtaataatag cacattttgg tgtaagaaca
     6301 gctgtgtcct caacaaaaca agaatccgga tactcttcaa gcgcctccag aacagtcact
     6361 ttaactccac atgccttcag tgcctcgata tatgcatcat gctgtttcag tgcaagttca
     6421 taatcgggtt tacctaactc aggcgatgat gtaattcctt ctaccattga ttttgaaggt
     6481 cttctaacaa taacatttct aaacataatt tcctccaaga taattttaat tttaccattc
     6541 atttcttaat atttttctgt tagtacacgt atactttata ttgtatacat tatattttag
     6601 tatcaaatta ggataaatgc aagtattttt ttggtaatat ttttgtaatt aaaaataaaa
     6661 aattaatgat ttttattatt aaatataata ttattaaatt tgtataatat cgtacaatta
     6721 ttttgtatat aatatattgt tttttggtaa ttttatgtta taatatttaa aaaaaataaa
     6781 atatggagaa aaaaatgtcg caatatttaa ccttaaaaga tcatgtatac aattatatat
     6841 catctaagat aaatgacggt tctttaaagc cgcaagacaa aataaatgaa caacaaattt
     6901 ccgatgaact tgaaataagc agaacaccaa tcagagaagc cctgattcaa ttggcatcgg
     6961 aaggatattt agaaaataga gcccgtaaag gttttcgcgt aaggagttta gattatgaga
     7021 aaacaaaaga actctatgaa ataattggat tgttggatgg aagaatagct tacatgacgg
     7081 tagatttaat taatgacaac cacttaaaaa atatgcgatt tctggcagat tcgatggcat
     7141 cggcaatcga acagggtctt aaccaaaaat attatgaact tcaaatagaa tttcataata
     7201 tatatactga tttatacaat aatcaagaaa tgattaattt attaaataaa ctaaaaaaga
     7261 attttatgcg caaatattat ttttttgaaa caaaaactga tgaaaaagct atactaaaga
     7321 aaacaaacga ccagcactat gaaattatca ggttatttga agaaaagaaa aaagaagaat
     7381 tggaacgata tatcagggat attcattggg ctgtcgacat agctatatat gattctttac
     7441 aaaattatta aaaaataacg gcactgacta agtgtcgtta tcttttatat tatagtcata
     7501 ctatgggtca tatactctta attctttatt cttaataaag gagctgtcat acaagttgct
     7561 ccgccatttc ccggaagaga tatgtgctgt ccttcatact cgtgaacttc tacgccctca
     7621 tttaacagtt ttttcttaat gtcgggattt cccttgagca atacaacctt tctaggctca
     7681 atagtaagta cattacatcc tagaggcata tattcgtcat ctattacttc aatcaatttt
     7741 atacctttct taattaacat ctgtctaaaa aacacaggca tatatctgga gtatactact
     7801 gcaagatctt tgtctatggg acttataaca gacattaaat gaagacattc ttcttcatta
     7861 ttgccatgag gcatcggcac tactattact tcatcaacca agtctttaac caattcttta
     7921 aactgcctga ttccctcatc gttggtacgg taaccccttc cgattgcaac tctgttatcc
     7981 tctaaccaca gtacatcacc accctcaacg gttccttctc cggttatctc gcccaaaatt
     8041 ggaactccta tactctctaa gtaaaccttc atagcctttg gttcgttttt tctggcattc
     8101 ttgcccatat ttagaattat cgcacctttt tcggtaaact taacactatc atgtgtataa
     8161 atagaatcaa gtccggattc ttcatccttt ggtaataatc tcacatccgg taccaatttt
     8221 tttattaaag cttcaaatgc atcgtattct tctacacatt tgttgtaatc agggcaagaa
     8281 aaaaaacccc acttctgcca attctcattc aagtattcct ggctgacata tgcttctctt
     8341 gcatgcttaa taactatagt ttggattttg tcaaccattg agcgactacc aacttccatt
     8401 tttagacctc tctttcatta ttgtatacaa tattttgtat attttatttt tgtttttatt
     8461 ataaataata ccagtagaca ttgtcaagta aattatactt aaatcaagta gttcttttta
     8521 tcgttaagga tgattattaa tttctcatac aaaaaaactc tacaaatgca taaaacattt
     8581 gtagaggata tatatgaaaa ttattcgtta acttttatat gccggctata atatctttaa
     8641 caacctcgct tgcaataatc agccctgctg cagaaggtac aaatgataca cttcccggta
     8701 ttgcacgtct ttgggtgcat tttcttgtgg ttccgggcgg gcatacgcag ctgtccttgc
     8761 agctgtcttc atcgcagctt ggagtcagag gtttttcttt tgaatatact acctttaaat
     8821 tttcaatacc ccgccttttt aattcatgac gcaatacctt cgctaagggg tctaccgatg
     8881 ttttgtaaat atcggcaact tcaaaggcag tgggatttaa tttgtttcct gctcccatgc
     8941 tgctgataat gggaatattg tttttttttg ctcttactat caattctatt ttagcggtaa
     9001 cagtatcaac tgcatctaca atataatcat aagaagttaa atcatattca tccgcattgt
     9061 caggcatgta aaaaacctgg taagttgctg tactggcttt tggatttatt tccttaatcc
     9121 tttctgccat cacttcaacc ttcttcctgc caatggtttt tcttgttgca attaactgcc
     9181 tgtttatgtt tgttatgcat actttgtcgt catcaaacag tgcaaaattt cctacgccgc
     9241 atcttgccaa agcttcaaca acaaatgtgc ctactcctcc gatgccgaat actgcaactg
     9301 tggaagagct taatttttcc attgcttgtt tacctaatag tagctgtgtt cttgaaaatt
     9361 cgttcatatt atcctttctt tttatgtcat tgttatgagt aacccataga gcgtcattct
     9421 gaaccctagg tgaagaatct catcttttaa aatctgagat ccttcgctta cgctcaggat
     9481 gacacgggct gcgccctcaa aattacaatt attaataaaa gttacaaatc tttgattgct
     9541 ctttatcacc aatttttaca tacatcaatc caggtgttgc tttttgatgt ttcctccaat
     9601 tcatcaaggg tgagctctat tgcagagtta ccgcttccac aggcaggaaa catagtttca
     9661 aatcttttca ttgatacatc caagtaaact tttacattat caggcagtgc aaaaggacaa
     9721 acccctccga cttcatgtcc ggtgaatttt tttgtatctt caggtgaaag cattcttgct
     9781 ttaaacccga aataatcttt gaattttcta ttatctatct ttgcatctcc tgctactact
     9841 attaccattg ctccatcacc gttataaaat gataatgatt ttgcaattct cgcttcctcc
     9901 gtatctaagg ccagagcagc caactctacg gtcgctgttg attcgtccat ttcttttatg
     9961 tcattttcct tgttaaattg ttttaaatac tctcttacct tttctactga catttttccc
    10021 tcctaatact tctttatgct atctatcgct atgtcaaata cttcttttat tatcttatca
    10081 gtttttccaa agtaatacaa tccttaagtt cttgaagcct gtcttttgac agaggaggga
    10141 ccccttccaa tttatatttt atatttagtt ccttgtattt atacactccc aatgtgtgat
    10201 aaggcagcag ctcgattttt tgaatcaatt tatcaggaaa agttttcact tcattctcaa
    10261 agtctttcat atctccttct gtatcattaa ttcccggcgt tacaacatgt ttaatccaaa
    10321 gctgtatttt atttctgatt accgcttcct taaagtccag ataatattca atatcgcagc
    10381 cggttatctt tttatatttt tcaggatttg aatgctttat gtcgagtatt gccaaatcgg
    10441 tatattttaa tatttcatca taatctccaa aacctactcc cgatgtatct ataaccgtat
    10501 ggattccttc agctttgcac agcataaggc aatccagcaa aaattttggc tgcctcaatg
    10561 gctcacctcc tgaaaaagtt atgcccccgt tattcttaaa ataaggttta taccttttcg
    10621 ctttttcagc aatataatct gcagtaaacc tttctttgag ataatattta tctgtcaagt
    10681 ttttattgtc gcctggagtg atgttttcta tccatgtgtc cgggttatga caataaagac
    10741 atcttaattt gcacccctgt aaaaacacta tcattctaag ccccggtccg tccaaagtac
    10801 ccaatgtttc tattgaatga agaaatccgg cagtcatttt tacatcctgt cgtggaatgt
    10861 tcttcctatt acatccattt gctgctcacg agtcagtctg ttaaagttta ctgcgtaacc
    10921 ggaaactctt atagttaagt tcggatattt atggggagct tccatggctt cataaagagt
    10981 atccctgtta attacattaa catttatatg atgagccccc tgagaaaaat atccgtccag
    11041 tatatttgac aaattatcta ttctgtgtct gtattttttt cccaatgtat cgggaactac
    11101 cgtaaaagta tttgaaattc catcttcgca aatatcttta tactttaatt ttgcaaccga
    11161 attcaaggat gcaagcaatc cgcttttatc ccttccgttc attggatttg ctcccggtgc
    11221 aaagggctct cctttttttc tcccgtcagg tgttgctcct gttttcttgc cgtaaacaac
    11281 attcgatgta atagttaata ctgaaagtgt atgctttgca ttcctgtagg ttttatgttt
    11341 ttttaattca ttagagaaaa attccaataa ttccgcagca ataccgtcaa cacggtcatc
    11401 gtcattaccg tacattggat attctccctc tatttcaaaa tcctcggtta taccgtcttc
    11461 gccgtaaaca ggttttacct ttgcatattt tatggcactc agagagtctg cagcaattga
    11521 aagccctgca actccaaagg ccatcaacct tctcacatca tagttgtgaa gagccataag
    11581 tcctgattcg taagcatatt tatcatgcat gtaatgaatt atgttcatag tattaacata
    11641 aagctccgct acatattcca gtactttttt gtagttgttg agcacttcgt cataattgag
    11701 tatttttgaa tttattttat ttatatttgt tattactgtt tctttactga tttcatctgt
    11761 accgccgtta atccctaaga gcagtgcttt tgccaaattg catcttgccc cgaaaaattg
    11821 catttctttg cctattgcca ttgcggatac gcagcatgca ataccgtaat catcaccgta
    11881 aataggtctc attatgtcat cgttttcgta ttgaacagca gatgtatcaa tggacatctt
    11941 agtgcaatat tttttgaaac cttcaggcag cctttccgac cacaaaactg tcatattcgg
    12001 ttccggtgca gccgacaaat ttgttaatgt gtgcaaaaac ctgaaagatg tcttagttac
    12061 caatgttctc ccatcaagtc ccattcctcc caatgcctcg gtaacccagt tcgggtcccc
    12121 tgcaaacaac tcattatatt cgggagttct taagtgacga accattctaa gttttaaaac
    12181 aaactggtca ataatttctt gagcttcttt ttcgctgagt ataccttctt ttatatccct
    12241 ttctatatat atgtctataa atgtgcttgt cctgcctaat gacattgcag ctccgttatt
    12301 ttctttcacc cctgccaaat atccgaaata gactgcctgt acagcttcca atgcatttga
    12361 agaaggtttt gaaatatcaa taccatattt agatgccatt gatttcatgt caaataatgc
    12421 tttaatctgt tctttaattt cttcccttgc tctgatgcta tcttcattca tagggaattt
    12481 aatattatta tattcttcct tctttttctc aaccagaaaa tctatgccgt acaaagcaat
    12541 tcttctatag tcgccgatta ttcttcctct cccgtatgca tcgggaagac ctgtcaaaag
    12601 tccggagctt ctggccaata gtatttcagg tgtgtataca tcaaatactc cttcgttgtg
    12661 agtttttctg tatttatgaa aaacattaca taactcatcg ctcatagagt atccgtaggc
    12721 ttccaaagac tgctccacca ttctgattcc gccgtatgga ttaatcatcc tcttcagggg
    12781 tgcatctgtt tgaaatccca cgattatttc attgtccttg tctagatatc ctggctcaaa
    12841 attattaatt cccgaaatat tatcggtatc cacatccaaa acatcttttt taagttcttc
    12901 tgtcataagc cttgatactt tttttaataa gtttttggtt ttttctgttg cgccgcttaa
    12961 gaattcttca tttccttcgt aagggctgta atttttttga ataaaatctc ttaggtcaat
    13021 ttcttttgtc catcttcctt gaataaaatt gttccactct ttcatagttc ctcctatggt
    13081 ttttccggga ggctatgttt tgaaatttaa aaagccgccc gggattagcc caggcggccg
    13141 acttttaatt ttattcacac ttcctcgtgg tactccactt ttccgccagt catgtgatac
    13201 tcttatgtta caataaataa cattttttgt caatacttta tgctcttaat ctttcagtct
    13261 cggatcaagc gtatctctca atgcatctcc gaaaagattg aatcccaata ctgccaatgc
    13321 catcactaat cccggaaaaa tagagaccca aggtgcttcc ataatatatg cacgccccga
    13381 actcataatc gagccccagc tgggggccgg aggcggcaca cctagaccga ggaaactcaa
    13441 agaagattcc actataatcg ctttaggtat ttcgagtgta gtaagaacaa taacaggtgc
    13501 catcgaatta ggaagcatat gcttgaaaat tatacgcgca tcactcattc ccagtatctt
    13561 tgttgcttcg ataaattcac ggttctttaa tgatataaac tgtcctcgca caagtcttgc
    13621 ataaccagac caactaacca gtgctactgc aaaaatcaaa tttgttaagc ttcgaccaag
    13681 tgccgcaaca aaggcaatgg caaggaggct tataggaaat gaataagcaa tatcggtaat
    13741 tcctgaaata atatagtccg taattccccc gtaatagcct gccaatgctc ccaaaattgt
    13801 gccgattacc aatgaaatac ctaccgcaat aaatcctacc tgtagggaaa ttctggagcc
    13861 gtaaacaatc cggctgaata tatccctccc gaattcatcc gttcctaaaa tattttcaga
    13921 gttaggtgct ttcaacatct ggggcaggtc aatggcatca tagggatatg gtgcaataag
    13981 cggggcaaaa atagcggtaa taaacaaaat taatattact gtagcaccaa aaacagccat
    14041 tttattagaa aaaagccttt cccaaaaatt gggctcccaa ctttccactt catcttccaa
    14101 attgtcttct ataataatgt catccatatt atactccatc ttttttaata cttttatata
    14161 aactagattt aataccttat cctggggtca acaagggcat aaagaatatc tgctattaaa
    14221 ttgcttatca ttaccgatgt tgcaagtatt aatactgagc cttgaacaac agggtagtct
    14281 cttgaaaaaa ttgaatcaac catcgtcctt cccaaaccgg gtatgctgaa aataatttcc
    14341 aaagtgaccg agcctccaat aatccagccc attcgcatgc ctaagagggt tatgacaggt
    14401 atcaatgcat ttttaagcgc atgcttgttt gttacaactt tttctctcaa cccttttgct
    14461 cttgccgtcc ttatatagtc ctgaccaata acttccaaca tgctggaccg catcatcctg
    14521 gctattaccg ctgcatccgt gagccccatg gtaataacgg gcagcactaa atgtttccag
    14581 gtaccataac ctgatatcgg aaacaaccct aatttatatg caaagaaata aagcagtatt
    14641 attccaaacc aaaatgtggg cattgatatg ccgaccaaag caaaagtcat agctataaaa
    14701 tctgctgact tgtttctgtt aactgctgca ataattccaa caggaacggc aattaaaaag
    14761 gaaagtagaa gtgccctcac tcctaaaccg attgttatag ggaaacgttc caaaacaata
    14821 tctgccacag gtctatgctg caaaattgag ctgcctaaat ctccctttac taaattactc
    14881 aagtaatcaa aatattgtat tatgagcggc ctgtctaaac ccatgctcct tctgacattc
    14941 tcataaactt caggagtcac gttaggtcct acgactaaat gcaccgggtc tcccggagcc
    15001 aaatgtaaaa ttaaaaataa aataatagtc aatcctaaca aaatcggtat catcataagg
    15061 agctttttaa atatatattt aagcattaat tcacctcaat cgtaattatt gcaaagcttc
    15121 cggatatacc ggaagctttg cctgctctat tcaacttcca aatatgtacc gtcattatat
    15181 tgaggtatcc aaggatgaaa cttgaaatct ttaacttctt ttcttactgc aatggtattt
    15241 tcaggtatat aaatcggggc ccatactgcc tgttcaatca aatgcttttg aacttcaaaa
    15301 tatcctttag atctttcatc ccatgtagga cttgtagctg ctttattaat taactcatcg
    15361 gtcttttcat cctgccatct tgaatggttc gggtatggga actggctcga taggaggaac
    15421 caatccaata tatcggcatt cggccagctg taaagcctta tgaaaagctc ctgttgacct
    15481 tctcttaaca ttgacgtata gcttgaactg tcatatagtt gaatttctgt ttcaattcca
    15541 acctgtttta gctggtcttg aagcacttcc gctaaacggc tgtactccgt agaattttct
    15601 gccgataatg taagcttgag cccgtctcca tagccggctt cctttaacaa ttgctttgct
    15661 ttctccacat catactcata tccatccttt ttactgtccg cataatattc gcttttcagg
    15721 gcaggaggca aataaccgta agcaggctct ccatatcctc taaaaacgta tttaataata
    15781 tcttctttat taattgcatg gtttattgcc cgccttactc tgatgtcggt aaaaggctcc
    15841 ttatcgcagg cataagccaa atatccaagt tttgtagcat ctcttctcat tacttctaca
    15901 tcgtcaattt cttcaactct ttgcaaaaat gatgcaggga catccctcaa tatatgtaca
    15961 tttcctgctt ctaactccat cagccgcgta ttttcttccg gtattgttct gaattctatt
    16021 ctgtcgatta aagccggtcc cctgttggtc atccattcag gtccccaatt gtaatcggga
    16081 ttctttacta ttataatttt atctccttgt ttccattctt tgaacatata gggtcctgtg
    16141 cctactacat aattaattcc atactcatca ccatactctt cataagcttc tttgtttact
    16201 attcctgctg ccgtatttga caaaacaaac aagagatttg gaaaaggttg atgcagcatt
    16261 atatctaccg tgtgttcatc ctttacttcc aatcccttta tcgctgcgaa atcgctttga
    16321 gaaggagatg cagtttcagg gtctaaaata gtgtcaaaag taaattttac atcatgtgca
    16381 gtcaaaggct ttccgttatg aaattttaca tcgtctctca aatgaaatgt ccacaccatt
    16441 ccatcttccg aagtttccca acttatagcc aaactcggtc tgtaattaaa atcgtagtcc
    16501 ctggaaacca gcctgtcata catataaatc aaagaatctg aataccagcc tgtttttatg
    16561 tgattcaggg tttccgcatc tctttcgtag gctataacca gggttgtctc ttttgccggt
    16621 tgctcaccgt tgttaccttc cggcgtttcg ccgacgggca catcattttt cgaacatcct
    16681 gaaaaaacta atgctgtaca cgataaaatc agtaccaatg caaacatttt cgaaatattt
    16741 tttttcattg aactaatcct cctcttcatt tgaatataaa tgacattcca ccatatgtcc
    16801 gtttcccagg ttgacagata caggttcctt ccttgaacaa tcttccctag cataaataca
    16861 tctggtatga aatcgacatc cctttggagg attagccgga ctaggcaaat caccctccaa
    16921 aagaattata ttcttacgaa agtcagggtc acatacgggg attgacgata acagtgcttt
    16981 tgtataggga tgagccggcc ttttaaataa ctcatcgctt tctgcaatct ccacaatcct
    17041 gcctaaatac attactgcaa ttcggttgct tatatgtttg acaacactta agtcatgagc
    17101 aataaacaga tatgtaagct taaagttttt ctgaagctcc tccaataaat taataatttg
    17161 agattgaatg gaaacatcca gtgcagacac aggctcatca caaacaataa attttggctt
    17221 tacagccaaa gctcttgcaa ttacagccct ttgcctttgg ccgccgctga actcatgagg
    17281 atacctcgat aaataatttt tatttaaacc taccatttca atgagctcaa ttactctttt
    17341 ttgccgctca ttcttatccc taatatggtg tatatacaag acctcttcta aggcacttcc
    17401 gatggtcatc ctgggattaa gtgagccaaa ggggtcctga aatatcagct gcatatccct
    17461 tcttatcttt ttcaattctt tcctgctgct cttcaggata tccttgcctt caaagaaaac
    17521 ttgtcccgaa tctaccttca gcatattcaa gactgcacga ccggttgttg atttcccgca
    17581 cccgctttca cctactaatc cgagagtctc ggcatagcct atattaaagc tgataccgtc
    17641 caccgccttt acataaccga catttttagg aatgagctct cctttgaccg gaaagtggac
    17701 cttcaaattc tttacctcta atatatttcc actcacacag tcacaccctt cccttcatat
    17761 ttccagcacc tgaccttgct ttttccaact tcgaaaatag gtggttcttc aaccaggcat
    17821 ttctcaaata catattcaca gcggctatta aaccgacacc ctgccgtaat ctcaccggga
    17881 acaggtaccg agcccttaat ggcatatagt tttttccttc cgcctttaat tcttggtatg
    17941 gaattcaata aacctatggt ataaggatgc ttgggctctt tgaataatgt tctgacatcc
    18001 gcatactcta aggcccttcc gcaatacata accataacat tatcagccat ttcagctact
    18061 acgcccatat tgtgagtaat taaaataacg gctgtattca tcttctcctt catctctctt
    18121 ataagatgta aaatttgtga ttgtattgtt acatccaggg ctgtggtagg ctcatcggca
    18181 atcagtatct ccggcataca ggcaagtgcc attgcaatca taaccctttg gcgcataccc
    18241 ccgctcaact catggggata ttgcttcatt cttttttctg ccaatggaat ttttacctgt
    18301 tttaacatat ccaatgattt tttcattgct tctttcttgg acaacttttg atgtgtacaa
    18361 aaaacttccg ataattgctc tccaattgta taaaccgggt tcagagaagt catgggctcc
    18421 tgaaatatca tggatatatt gttaccccgt atttgcctga ttgtatcttc atctactttc
    18481 agcaaatcca ggctgccaaa aaacatttct cctccggtta ttttcccggg cgggtttggt
    18541 ataagcttca taatggataa agaagttaca gtcttgcctg agccggactc tcctactata
    18601 ccaagtgttt ctccccttga aagattaaag ctgaggttct ctacggcagg tatctccttt
    18661 ctgccgctgt aaaaacaaat gctcaaatcc cttatttcca acacatactc gctcatagtc
    18721 aatcacctaa tcttcaaaat aaaaataata acctgcagga gactatgctt ctccgaatat
    18781 aaataaaaga tcaaatcaaa cagcaatctt tatgagaaca ttttcctgta ttgtgaaatc
    18841 aactattttt aataagaatt attaatgtaa attaatttag aaagtgatag attagaaatg
    18901 taaatttaga caacgctatt attaatttag tattgcaatt gtatcactaa ataatataat
    18961 aggtcaatac ctttatactt tttctattat ttgatttatt tgttcttcta tttgttttat
    19021 catgaattct tctgttactt tttcttcata tagttttgac attgccttgt ctaattgatt
    19081 tttagtattt tttactgtat ctaatgattt tcttattccg gactgaacaa cccagtcaaa
    19141 aataaggttg tcaaaaaaac agtctgcaaa attttcaaag gtacctactg ctatttcagt
    19201 tccagtcaca attgttatgt cggataattc tttcttgaat ctccctaaca ttctatgggt
    19261 ttgctccgca tacccccttg cattttcaat attgttgctt ccatctgaat tttcaaagtt
    19321 ttcagcagct tctaactctt ttattgtcat ttctatagct tccagtgctt tttcaccata
    19381 acaaattgct tcgcaaattt cgattatatt ggcattcata ttttcttttc tttttattag
    19441 tttttttaaa gcctggccgg tttcattgtc ctctatatta atagtttcta tctttttgtt
    19501 tattaattct tcatactcag catcgcatcc gtttaatttt tctatacaat ctacaatctt
    19561 tttagtttcg ttaactagat aattcatgtt gtttttacat tgttcatatt ttaaccttgc
    19621 ttttaatagc ttctgctttt ccttgttgtg tttatcttct tccgtcccca atatttcata
    19681 aaacaacgaa ataatattat ctgattttag ctttaaagca tcctgatatt ccttctccaa
    19741 ttcgcccgaa agcttattta gaagaagttt ttctttaata atatcttgct ccgtttgttt
    19801 taaaagcaaa tttaatttat tctttttttg tatgcatttt ttcatgtcgc ttattttttc
    19861 attgtattta ttatgcatat tcttgctccc gccctttgct attgttactg ttaacttttt
    19921 tgattatatc atacatctta tggcgattca aacagttttt agtctgaaaa aacgggaata
    19981 cgtttttatt tattattaaa aactattctt gatacaaaat tatcaaatag agtcaggtaa
    20041 ctttttatta attaaaatat tgaataatat cttatcttac tgtataatga attataaatt
    20101 aaaaggaggc aataaatgat tacaaaagac atgacaattc gtgaagttat tatgaaaaac
    20161 caggaagctg ccgaaatatt aatgacattt ggaatgggat gtgtaggctg cccaagtgct
    20221 caaatggaaa cattggaaga agccgcagca gtacacggaa tgaatttaga tgatttatta
    20281 gctgccttga ataaataaga ctttacaaca agcagtcaat ctgcccagca aaaaccggca
    20341 taaagccggt ttttatattt aaaagcaata aaacagctgt ttatattgct tatttatatt
    20401 atttacatta ttaaaaatat taatatgtaa atatcttagc catttagtta ataatgtaaa
    20461 ctgcatcatg ttctatctgt aactcttcaa taaaactcat aaacaaccct tacaatattt
    20521 catccaactt gcttttattg aatcctacaa ctacttcatc atcaatcata attaccggaa
    20581 cccccatata tcccaaggca attaattctt tttttgcttc actatcagcg gaaatatttt
    20641 tttcctcata ttcatatcct ttttccttaa tgtattcctt tgcagcagtg caatttgaac
    20701 atgatgagcc ggtataaatt tttatttttt tcattttatc ctcctgttgt tagtatgcat
    20761 atcgcctaat ttttaattta ttttaccata ttacttcatt taataccata aatatttaac
    20821 ttaaattttt cacaaaaacc atggctcgga ataatatgaa ttagattagc agaaaggatt
    20881 gaaataaatg aaagaatgta ccatatacat agatgtgccc gagaagtttt tgaagggtgc
    20941 aggtattcca aattcccggt ttattccctc ttatatttat caaataaaaa ttattcttaa
    21001 aaataacagc agccaaaaga aacaatgctc ctttagcgct actatgggaa atggaatcag
    21061 gtatttggaa aatatcaagc atgaaggtcc tgacattaac cttttagata cagtaagtgc
    21121 cgtcgaacca tgtaaagaat tggccaacga taatgtaatt attttcggaa acaatcttac
    21181 tctatccccc aattcaacaa atacaataat atttgacgga gcactatgcg acaagtatac
    21241 aattaacagc attgaaaatt caggtgataa aatacctcac agaagcaaaa tcaattttta
    21301 tgctcactta ttagatgaag acaaaattga tgactcatat tttatttcgt caactgctat
    21361 ggactccgat atgattgtaa aatgcgatga tgaaaagcta aatgcaggcg atattactaa
    21421 atattatatt gacatatgta caggacaata tgacttagca agaaaagttt atttaagaag
    21481 tatactggat gcaggacttg agtacatagg tgattcgtgt aatttagaac cgagaaatat
    21541 atatgaattt aacggcaaaa ccattataaa atgggatata ggcagccttc aaccttcaga
    21601 aataaaaaga atcggataca aagttaaagt aaaaaatgat gcaaaaggta aactaacaaa
    21661 taaacttaat tcaaactgca taaacaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnaaa atgatgcaaa aggtaaacta acaaataaac ttaattcaaa ctgcataaac
    21841 aactctacct atactcaatg tccggtaagc tgtaaataca ctattgaggt atgttgaaag
    21901 aaaatcagga gacgttcaat taaaataaag aaatcgtctc ctgttttact ctaatattat
    21961 tttatagtag tcagtatcta atatcattga tgaatattta aatttattta taactgtttc
    22021 cgctgtttct ttaatatagg agctttcaat attttcatcg gtaaatgaag ttacctcgtt
    22081 tgttctggca ttgtaaaatg ctttgtccgt aatgaagctc ctgtccgaaa acataactaa
    22141 gggctctgaa tccgaaaaga tatcccttcc cataagaagc cttgaatcat attccaaccc
    22201 cattaaattc gacaaggtag gaattatatc caaacttgaa caaaccttgt ctattttaac
    22261 aggctccatt cccttgctcc aaagaataaa aattcctcta tccaaatcaa catctgattt
    22321 gatttcttta ccggcaagct cgcttatttc attcaatgtc aaaccgtaag gataatggtc
    22381 aggacttatt gctatgagag tatcttcggc aatccctttt ttctccagct cctgtatcag
    22441 tttctccatt gctttatcca actcgatatt acatgccaag tatgccttta catttttgga
    22501 gtatggcaaa ttttccacca gacttctgtt tttagcagct atatagttgc ctgtaaaggt
    22561 atactgcaaa tggccgctaa ctgtcatata gtacacgtgg aaaggttgag aatctatgta
    22621 ttctggtacc gttagctcaa tcatttccaa atccgactcg ggccaggttt ctgttacatt
    22681 aagaccgttt cccaaacctt tatatgtgta acctaagtta ggatgagatt tatgtctgta
    22741 ataataatca aaataatggt tgtgataggc tttggtttca taaccttctt ttttaagctg
    22801 atttcccatt gcaaagggca tatagttttt ggaagattta taaaagctcc atatgccttc
    22861 ctttggcaaa agtcccgtac aagctacata ttcaccgtcg gaggtagaga ctccccaggt
    22921 aggagtataa aagttattaa acttaaaacc ttcctcttgc attttgtata aagtaggcgt
    22981 tatatccttt ctgactgcat atggcgaaaa gccttccgca gtaatcaaaa tcaaattctt
    23041 gcctttaaat ttacctgtat attcattttt ctttgtcggc ttgacgctgc tgaaatactt
    23101 atgcatactt cttattgctt catttccttc tttttttatc aattcttcaa aaggaatatc
    23161 catagtattg tattctgcaa cctcaattgt cggttcttcg gattgaggct ctccgggtag
    23221 ctcatctgca gggtccggct cttcagattc aattatttcc gaatgctccg gctcaacggc
    23281 aaatacaatg cttttcaata tattttttat gtctaatctt cctgttgtta aaagtccgaa
    23341 cttatcaacg gattctacaa ccaaaaaggt ttcggaatat aaatatgatg ggctgagcat
    23401 acccatatca gaagaaagca cggttataac aactgcagcc tgcattatta ctgcagatac
    23461 agcaactttt atgatatagt ttttatgtat tttatcaaat tgtaacttcc tgtgataata
    23521 aaccaatacg ccaaggggaa gaaatattgc aaacaattcg gcaatattgt ttcgaagtgt
    23581 gtcagtcatt atgtctatga agcttaaagc ctttgctgtt cccacaaaga tagaataaaa
    23641 ggaagtaaag gtataaaata tcctgtaata aattaattga gtgccgt
//
LOCUS       DAOSHP010000019        23206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1758_length_23206_cov_9.092566, whole genome shotgun sequence.
ACCESSION   DAOSHP010000019 DAOSHP010000000
VERSION     DAOSHP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23206
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_23206_cov_9.092566"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..498)
                     /locus_tag="PLB33_03330"
     CDS             complement(1..498)
                     /locus_tag="PLB33_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOG62584.1"
                     /translation="MSLYKQWTENLEAIKTQGEYNEFFKGYMEKETECYRRILENKTA
                     VIEGKIVELAEENKMSALEYIGFLDGANTSFKKELVLDDLTEESEIKAEFDFEKLLWN
                     MHDAKAEWLYGMKEWENIFDEDKRKQIKKEFNRSKIVVKGEKIGRNDPCPCGSGLKYK
                     KCCGK"
     gene            complement(692..1117)
                     /gene="mscL"
                     /locus_tag="PLB33_03335"
     CDS             complement(692..1117)
                     /gene="mscL"
                     /locus_tag="PLB33_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOG62585.1"
                     /translation="MWDDFKKFAFQGNVLDLAIGVIMGGAFGKIVTSVVNDLIMPVLG
                     YLMAGIDFKDMKYVMSEAIMEGDTVIKPEAAIMYGNFIQNVVDFLIVALSIFLFIKLI
                     NKSKEKLKKQEEAAPEPEAPPAPTQEELLTEIRDLLKEK"
     gene            complement(1239..3830)
                     /locus_tag="PLB33_03340"
     CDS             complement(1239..3830)
                     /locus_tag="PLB33_03340"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOG62586.1"
                     /translation="MMLKRIAAISAAAILLLSSSAYGSSLYTVYDLSEEIRLSKSITY
                     ERIEKYTSSGWMNINVIRADLTDEHTEIKPINNEDGISNRSTLSSMIRSSGAVAAVNG
                     DFFYMGDPTYTYGPIIRDNKLITSPLPFNDGYPTVSKLKDEIDISVWNPKITLYGSDS
                     TEFNVVVMNKTSNIEWGPTILTSDWNKTSPGYTTQDIVEVVVVDNIVSSVRKNQPSTI
                     IPENGYIVASSNPATMELMLNSFKEGHPVSLNIELDFSPEDIDWSFGALNYLVKDGRE
                     NVISSQVLGVNPRTAIGFNKDNTEIILVTVDGRHRDYVGAKQTELAKIMIELGAYNAV
                     NMDGGGSTTMAVDFLRNANVTVVNFPSDGRERKIASGVGVFNNYPDSNKVDKIEITAS
                     QNTVFNKTETNLDLKFYNEYYTPVEFDLKNVDYTVSPAGAGKVVNNVFMPEKPGKATI
                     TAHAGNAEGHIEIDILDKPVALKLDTDSLTLGFNDKYELGTVLGIDKDGNSAYIPWKY
                     ITASYRNRVGKIEDGVFISSDIANTGAVTLTFGDAIKHIQVKVGYRYKTLNRFENLDN
                     LKLSLYPEESAGSIYITKDFVKEGKNALRLDYDFTKMTDQSIAFVEFGDEGEGIKLED
                     KPLAIGMWVYGDNKNHWLRTRITDAYGTMVKIDFEEEVNWTGWKWVEAKIPEGIAYPI
                     TLKNIYLAEINEARKDTGTIYVDNLRILYEPKDKQLGLREETKFEDKMKTSSIKNYTE
                     KLTITQGNYYYEGIGNIVYFEGIISNGTMSSADTTMWNNIKSFMDFEDKVLVLSMNGD
                     IDNINDKREIKILNEILEKASQNNDVFVVFKGENESTVIENKVRYITYDDSFEIGISE
                     GKVSYKN"
     regulatory      4148..4243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4408..5586
                     /gene="metK"
                     /locus_tag="PLB33_03345"
     CDS             4408..5586
                     /gene="metK"
                     /locus_tag="PLB33_03345"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOG62587.1"
                     /translation="MRKWLFTSESVTEGHPDKMCDQIADGILDAIMEQDPNGRVACEA
                     ITKTGFVLVAGEISTSCYIDIPHLVRETIKNIGYDNALYGFDYKTCAVFTSIEEQSQD
                     IALGVNEADEYKKNNNDINDMIGAGDQGMMFGFACNETPELMPMPISLAHKLAKKLAE
                     VRKNNIVDYLRPDGKTQVTVEYHDNKPVRIDTIVISTQHNPEVNIDTIKKDLIEIVIK
                     PTVPNELLDENTKYIVNPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKD
                     PTKVDRSAAYAARYIAKNVVAAGLADKCEVEVAYAIGVAKPVSIMVETFGTNKVDDEI
                     IQKAITENFDLRPAAIIEKFNLRRPIYQKLAAYGHFGREDLDLPWEKTDKTDELKKYL
                     "
     gene            5637..7133
                     /gene="malQ"
                     /locus_tag="PLB33_03350"
     CDS             5637..7133
                     /gene="malQ"
                     /locus_tag="PLB33_03350"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOG62588.1"
                     /translation="MFERSTGILLPIFSLPSKYGIGTLGKEAYKFIDFLRSAGQKYWQ
                     ILPIGHTSYGDSPYSCFSVFAGNPYYIDLDVLFEEGFIEETDLKGIDFGSDDERIDYK
                     KIFETRYGILKKAYLNKGMSCDLASFTCNNPWVSDYALFMSLKYKNNQLPWYKWDEMF
                     IKRDINKLQKYKNDFYDEINFWIFLQYIFFEQYFKLKDYANSSGISIIGDIPIYVAED
                     SVEAWTEKKLLMLNEDNLPICVAGVPPDDFSDDGQLWGNPVYNWEYLKETGYMWWIDR
                     IAFSFKLFDVVRIDHFRGFDEFWAVPYGSENAVNGSWMPAYGKQMFQTLKKSLGNLNI
                     IAEDLGIITDSVIKLKNDSGYAGMKVLQFAFDGNNDNPYLPYNYEENDVAYTGTHDND
                     TLKGWFNKLTLKEKQYVLQKLNINMVNEKDDNNLVYELIKAIYKSKASLCIVPLQDFL
                     CLGSEARVNIPSTLGNNWRWRLKNSMITDELAEKIKALKTERDVQNFN"
     gene            complement(7319..9091)
                     /locus_tag="PLB33_03355"
     CDS             complement(7319..9091)
                     /locus_tag="PLB33_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOG62589.1"
                     /translation="MYKFNSQSEFYRYPQGGIKSNEIITFKILVKRNICAPELLIEKR
                     HDYSRTFHDKIKMHWISQEKNYDIYQAMLSIEEYGHYYYSFIFDNEKSKDYELLIYDD
                     KYITPNWAKGGIIYHIFVDRFFRTKIIKKKGAIIRNWGETPYYLPDKKGEILNNDFFG
                     GNLDGIREKLPYIAKLGVSIIYLSPIFEAYSNHKYDTGNYHKIDPMFGTENSLKKLIK
                     EAKKYNIHVILDGVFSHTGDDSIYFNKYGRYDSIGAYQSKDSPYYDWYTFNNWNDDYV
                     SWWGIKTLPEVNENNDSYINFITGKNGVITHWQKTGIMGWRLDVADELPDEFIIALRN
                     SIKKNNEDALIIGEVWEDASNKYSYNKLREYLCGKQLDSVTNYPLRKGIIEFVKNKDC
                     TNLNHIMNKVIETYPPETVNCLMNVLGTHDTERILTILGSKRVPSSRSEMAESKLSYE
                     ELEQGIKFLKIAVLLQMTLPGIPCIYYGDEAGIEGWDDPFNRRCYPWGKENKEILDFY
                     LFITKLRRTNKIFAEGKYRCLIHDKEVFVFERFNENERIIIAVNLSQKEITLKFKEDM
                     IEYTNNIKNNIFNIEKENYLILLS"
     gene            complement(9170..11590)
                     /locus_tag="PLB33_03360"
     CDS             complement(9170..11590)
                     /locus_tag="PLB33_03360"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOG62590.1"
                     /translation="MKDSKIDATLINRLIDEEIIRYWNTEPEKATINQIYRAFSIVIR
                     DLLLKKRNEFKRMQNEHKQVYYICMEFLIGKSLRNNLFNLGIEDEAKEALKKYNITPE
                     ELYSIERDPGLGNGGLGRLAACFMDSLATQDYAAVGFSIKYEFGLFKQKLIDGWQTEL
                     PDDWLTTSDVWLMPRTDERVIVKFDGTIREEWTGEGLKIHHDCPTEIEAIPYDMMISG
                     YNCKGVSLLRLWESRSINSIDMNLFSQGDYIRALSEDSMAEAISKVLYPADDHNEGKI
                     LRLKQQYFLVSASAQNIVKNHMKKYNSLDNFSEKNAIHINDTHPSLIIPELMRIFMDE
                     YGLSWEKAWTIVVNTVSYTNHTVLKEALETFDNNIVQTKLPRIYMIIKEINRRFIEEA
                     SAKYNNRDKAFNMSIFGYGKVNMANLCIIGSHKVNGVARLHSEILKRTVFRNFYEYTP
                     DKFISITNGITHRRWLNQANPPLGALLDDLVGPGYRTSPERLNMLKHYKDDDSVLNRL
                     DEIKYTNKKRLAQYVKTNNHIDLNIDSIFDIQAKRLHEYKRQLLNALRIISIIIDINE
                     GNTSHVVPETFIFAAKAAPGYHRAKEIIQLIYALGEYINKNTFIKKYINVVFLENYSV
                     SLAEIIIPAGEISEQISLAGKEASGTGNMKFMLNGAITIGTYDGANVEIINEVGEDNF
                     FLFGMKDYEVEKLWKNGYSSVIYYQKSLMLRKIIDFLNKGISGKSFESISRYLIGGPV
                     VDDPYMCFADFDSYMEVHQKASQLYFNNKKKWNQMSLMNIASAGVFSSDRTIREYADN
                     IWGIKPVV"
     gene            complement(11577..13034)
                     /locus_tag="PLB33_03365"
     CDS             complement(11577..13034)
                     /locus_tag="PLB33_03365"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOG62591.1"
                     /translation="MNKLKVLYVASEAAPFIATGGLGEVAGSLPKALVEKYKDKIDIR
                     IVLPLYQGIVDRNEFEFIGKTSVPLAWRQQYCGVYKTILNNVTYYFIDNEYYFKRSEC
                     YGHFDDAERFAFFSKAVLYILPMIKFMPDIIHANDWQTSLIPIYLSAKYSGYEGYRDI
                     KSIITIHNIEYQGVFDLAISEDVFDLHGKEKGIIEFKGAINLLKGALETAHFISTVSE
                     SYSKEILDDYYAHGLAEIIQKNSSKIKGILNGIDTEKYNPEDDTEIFENYNAESIQKK
                     YLNKINLQSLSGLPQDEDIPVIGIISRLVKHKGFDLIKESMEEILKEKVQFIILGKGD
                     RGYELFFKYLQESYRDKIYVRIGFDSDFAKKIYSGADLFLMPSISEPCGIAQMISSRY
                     GTVPIVRETGGLKDSIKDASLGKGNGFTFVDETPQALCEAVKRALIVYEKKEDWRKLV
                     ETVMKMDFSWRKSAEKYFDLYSSLANFEENNNEGF"
     gene            complement(13045..14163)
                     /gene="glgD"
                     /locus_tag="PLB33_03370"
     CDS             complement(13045..14163)
                     /gene="glgD"
                     /locus_tag="PLB33_03370"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOG62592.1"
                     /translation="MNALGIIFSDMQDWSMTELASHRSVAAIPFGGRYRLIDFPLSNM
                     VNSGINKIYLITSSNYRSLMEHVGSGKDWDLSRKKGGILMIPPYSAGENHGLYKGRLD
                     ALKRVLDYIKFSKRNYVVLSDSDMIYDINYNKVIQYHEEKNSDITAVYIKTDINKSDS
                     IHKILYEFDEDGRADDILVYPVTSGNYNIGLNTWVMNKEFLESLIYDAIARNYKDFSF
                     DVLSARVHSLRIYGYEYTGFFAHIDSLQSYYENSMKLLNMDTLQNIFYSNTGPIYTKT
                     KDTAPAKYCKDAVVKNSLIANGCIIEGTVENSILFRDVRVGKNAVVKNSIIMQHGRIS
                     DNAKLDYIITDTYTFVNKDRVLLGYSNCPFFIKKNSVV"
     gene            complement(14165..15319)
                     /locus_tag="PLB33_03375"
     CDS             complement(14165..15319)
                     /locus_tag="PLB33_03375"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF003670.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOG62593.1"
                     /translation="MKLAKKECVVMLLAGGQGSRLGALTTKIAKPSVPFGGKYRIIDF
                     ALSNCVNSGLDTIGVLTQYQPLILNSYIGDGKSWDLDRMYGGIQILPPYQRVGDSEWY
                     LGTANSIFQNTNFIDQYSPEYVLILSGDHIYKMDYSLMIDFHKRMDADCTISVIDVPI
                     KEASRFGILSSDDSGRIIDFEEKPKRPKSTQASMGIYVFKWNKLKKYLEIDDKNPDSS
                     NDFGKDVIPLMLRSNEKLYAYKFKGYWKDVGTIDSLWQANMDLLGENGFELSEDWIIY
                     SRHAEKPPHYVGKNALINNSLVSEGAVILGNVENSVIFSGVVIEENALVKDSVIMADV
                     KINKNCIINYSIIDEMVTIGANLKIGKPKGENSEITVIPRESVINKMQEE"
     gene            complement(15306..17222)
                     /gene="glgB"
                     /locus_tag="PLB33_03380"
     CDS             complement(15306..17222)
                     /gene="glgB"
                     /locus_tag="PLB33_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOG62594.1"
                     /translation="MKKNDLPIYLFHQGTNYYSYNFLGSHIIENIGTVFRVWAPNAKN
                     VHITGNFCNWQKSAEYEMKKISDSGIWELFAPGIKEGELYKYIITAADNTIIYKADPY
                     AFSSQTKTETASIVYNINKYKWNDTNWFEYKKRINLYEIPINIYEVNLPSWKRKENGD
                     FYTYREMAPLLINHVKKHYYTHIELMPIMEYPFDGSWGYQVSGYYSPTRRLGEPDDLK
                     YFIDQCHQNGIGVILDWVPAHFPKDEHGLYKFDGDFLYEYKDSEKREIKGWGTHKFDF
                     GRTEVQSFLISNAVFWLDVYHADGLRVDAVSSMLYLDYDKKEGEWTPNIYGGNENLEA
                     ISFLKKLNESVFKNYPNTMMVAEESTAWPMVTKPTYLGGLGFNFKWNMGWMNDMIEYM
                     ETDPFFRKYKHKNITFSLHYAFSENYILPLSHDEVVHGKKSILNKMPGPYEEKFANVR
                     VLLGYMMAHPGKKLNFMGYEIGQFTEWDYNKEIEWFMIEYEYHGKLNTYIKNLNEFYL
                     KRSELWEVDYSWSGFKWISNDDYEQNIISFKRINKNGDELIAVVNFSPTERHNYIIGV
                     DEGIYTEVFNSNDEEFGGTGTGNKDRLISSQGSVHGYNSFVSLTLPPLSIIFLEKETK
                     YKVNKEGGKIYEIS"
     gene            complement(17402..18244)
                     /locus_tag="PLB33_03385"
     CDS             complement(17402..18244)
                     /locus_tag="PLB33_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62595.1"
                     /translation="MRRLISILLIAMILSTGCTSLDMADENIVKDVSDIITDTVFNGA
                     GNEKAERKESHTVKSENKNTLNLMSSVGDINIVPHQSDDTIININITAKAKEKKKAEE
                     IIDNYIYKINEEGSSIVIDTTFNELPNDINLVTDLTVYLPSTIQNLEVTANVGDVNIN
                     SFIGNMDIKNNVGEVILDKSNGSYNIKADVGGIILKDCIAVGNSEFKTSTGDIELSFE
                     SISKAVSIIAETGVGNIEVSLNDDSGYHAVINEFMKDERIQTKHDQGTSINLTTGVGK
                     IEFN"
     gene            complement(18257..19036)
                     /locus_tag="PLB33_03390"
     CDS             complement(18257..19036)
                     /locus_tag="PLB33_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62596.1"
                     /translation="MLNLIKYEFIRKYRLVSMILITMLALNLFLLTRGVGGSMLFLVL
                     SPIVLYVFYLADIIKMYSDDLNKKTGYMLFMTPNSGYKIISSKLLAAILEGFALVLIY
                     FIFILINGTYIIISAGNEIDYSQIIKAVDNLLSGSLGFNLGHVLVFLLAALAFLIAFI
                     TTVYTAMTIRKSIFSEIKFGGLFSFIIFLLINWVLSFLSSKFYDIITPYYDTVDTISS
                     TGRVSAGGLAMILLPMIAVLIIQAVSLTGISGYLLEKKINL"
     gene            complement(19029..19736)
                     /locus_tag="PLB33_03395"
     CDS             complement(19029..19736)
                     /locus_tag="PLB33_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62597.1"
                     /translation="MNKLSVLKITDLTKRYYNKTALENVSLDFEQGKIYGLLGPNGSG
                     KTTLMKVVAGLHKQTSGSVLINDEMLSYKSKAYVSYMPTENYLYENFKVIDILKYYNT
                     MYEDFRYDYAVNLLNDIDVNSDIKISNLSSGQIGKLKTVIALSRDAQIYMLDEPLNGV
                     DVLSRDTILDLIAKSHSENKIIIISTHLVSEIEKILDTVIFLKEGIVEVFGDAEELRQ
                     ERQLSVEGIYKEVFRNA"
     gene            complement(19729..20100)
                     /locus_tag="PLB33_03400"
     CDS             complement(19729..20100)
                     /locus_tag="PLB33_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG62598.1"
                     /translation="MEFNNNIPIYLQVIEKIKQDIVSGKLKPGEKMPSSRDYSNELGI
                     NFNTVARVYRELENEEIVFTKRGLGTFITESDQIIEKLRYTMAKNQITAFIDGMKQLG
                     YTKEDMIKFIETENNYLEGNE"
     gene            20304..21377
                     /locus_tag="PLB33_03405"
     CDS             20304..21377
                     /locus_tag="PLB33_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOG62599.1"
                     /translation="MHKKLFIPGPVEVKEDVLSKMATPLIGHRSEDASNLQRDISNNL
                     KKLFYTENEILLSTSSGTGLMEAAVRCCTKERAAMFSIGSFGDRWHEMAVSNGINADL
                     FKSELGSITRPEMVDDVLKTGKYDLVTITHNETSTGVMNPIDKISNVIKKYPDVIFCV
                     DAVSSAGGVKIETDKLGIDICITSSQKALGLPPGLSMCTISERAVEKARYIKNRGYYL
                     DLVQLYDTIKKKDYQYPSTPNLSLMFALNYQLKLIMQEGLDNRFNRHFAMAEYTRSWA
                     KKNFALFADEKYASNTLTTVKNIRNISVKDLNIELGKRGFAISNGYGSLKEKTFRIAH
                     MADLTLEDVKELILNIEDILSIS"
     gene            21401..22180
                     /locus_tag="PLB33_03410"
     CDS             21401..22180
                     /locus_tag="PLB33_03410"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOG62600.1"
                     /translation="MYDLNKISSYLNTNTIGQSIIQYDNLNSTFAKAKNIFSTCPDGT
                     VVLSENQSECILRFGNEWLCSPDKNIYLSIILKSINNNYLVPLTDVVACSSILKSIEE
                     LYNLDCRIKWPNDIFINNNKISSVKSEIIGKGAGIILSLYINVNMEEKIENLKATSIK
                     IEINNDVERELLIGTILNNIEYHYVEMINTGKAYHSVDIYNENLLFNNKVVGVIKRGR
                     KTVRKAVAKNIDSDGWLQVINEKGEEEILDPGETIIQYEET"
     gene            22167..23171
                     /gene="dusB"
                     /locus_tag="PLB33_03415"
     CDS             22167..23171
                     /gene="dusB"
                     /locus_tag="PLB33_03415"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOG62601.1"
                     /translation="MKKLKIGSIELDTNVVLAPMAGITDITYRTICKEMGAGLVHTEM
                     ISAKGLYYQDRKTEELMKINKNNRPVSMQIFGSDADIMSYVVEKYINSRNAVDIIDVN
                     MGCPAPKIVKNKDGCFLMKEPELAGKIINKIKRTTKKPVSAKIRSGWSKDSINAVEFA
                     KILEFNGLDMVTVHGRTREQYYSGKADYRIINEVKKSVNIPVVGNGDIFSPHDAVKMI
                     EETNCDAVMIGRGVLGNPWLIMNTVKILNNDMNFYTPSPLDKINMIKRHASMLADELN
                     EKVAILEMRKFAAWYMKGMKNSSQIRNIINKITSAEELYKILDKYIEVYCLNDLNGLN
                     "
BASE COUNT         7333 a         4134 c         3652 g         8087 t
ORIGIN      
        1 ttattttccg cagcactttt tatattttaa tcctgaaccg caggggcatg ggtcatttct
       61 tcctattttt tcacctttta caactatctt ggagcgattg aattcctttt ttatctgttt
      121 tcttttatct tcatcaaaga tattttccca ttccttcatt ccgtaaagcc attcggcttt
      181 tgcatcatgc atattccata acagcttttc aaagtcaaat tctgctttta tttcagattc
      241 ttcagttaaa tcatctaaga ctaattcttt tttgaaactt gtgtttgcac cgtctaaaaa
      301 ccctatgtat tccaatgcgg acatcttatt ttcttctgcc aattcaacta tttttccttc
      361 aattacagca gttttgtttt caagaattct tctgtaacat tccgtttctt tctccatata
      421 ccctttaaaa aattcgttat attctccctg ggttttaatt gcttctaagt tttctgtcca
      481 ttgtttatat aaactcataa cttcctccaa aaaatatact gttcttttat tttatcatga
      541 aaatggaaaa attcaataaa aaaaagcgct ccttagagcg cacaatttac aaaccccttt
      601 gccattctta accgatgaga ttcttcaggc cgtgccttca gaatgacaaa ttcaaaatat
      661 actgccatta atggtgcata ataggattat cttatttttc tttcaacaaa tctcttattt
      721 ccgtcaacag ctcttcctgt gtaggtgccg gaggagcctc aggttcagga gcagcttctt
      781 cttgtttttt tagcttttct tttgatttgt ttattaattt aataaacaga aaaatagata
      841 atgctacaat caggaagtct acaacatttt gaataaaatt tccatacata attgctgctt
      901 caggtttaat tacggtgtca ccttccataa ttgcttcgct cattacatac ttcatatctt
      961 taaaatctat tcctgccatt aaatatccca aaacaggcat tatcaaatca tttaccacgg
     1021 atgttacaat tttgccgaaa gctccgccca ttataacacc gattgccagg tctaaaacat
     1081 ttccttgaaa tgcaaatttt ttaaaatcgt cccacataac ttgtcctcca taaataagat
     1141 taaccgatgg taaggggata cctttatgta cggttataat atattatacc caattttatg
     1201 taatatcaac ataaaattgg gtatgtactt caaatttgtt agtttttata tgaaactttt
     1261 ccttcggata tgcctatttc aaagctgtcg tcataagtga tgtatcttac tttattttca
     1321 attaccgtac tttcattttc tcccttgaat accacaaata catcattgtt ttgtgaagct
     1381 ttttcaagaa tttcatttaa aatttttatt tctcgtttat catttatatt gtcgatatca
     1441 ccattcatgg ataataccaa taccttatct tcaaaatcca tgaaggattt gatattgttc
     1501 cacattgttg tatcagccga actcattgtc ccgtttgaaa ttataccttc aaaatatact
     1561 atatttccga ttccttcata ataataattt ccctgtgtta ttgttaattt ttccgtatag
     1621 tttttaattg atgaagtttt cattttatct tcaaatttag tttcttctct taagcccagc
     1681 tgtttgtctt tcggctcata caatatcctt aaattgtcaa catatattgt acctgtatct
     1741 tttcttgctt cgttaatttc cgccaagtaa atatttttaa gtgttatcgg ataagcaata
     1801 ccctccggta tttttgcttc aacccatttc catcctgtcc agttaacttc ttcttcgaaa
     1861 tcaattttca ccatagtgcc gtaggcatca gttatccttg ttctaagcca atgattttta
     1921 ttgtcgccgt aaacccacat accgattgcc aaaggtttat cttcaagttt gattccttca
     1981 ccttcatctc caaactcaac aaaggcaata ctttggtctg tcatttttgt aaagtcgtaa
     2041 tccaatcgaa gtgcattttt cccttctttt acaaagtcct ttgtaatgta tatagaacct
     2101 gccgattctt caggatacaa gcttaatttt aagttatcaa gattttcaaa acgatttaat
     2161 gtcttatatc tgtatcctac cttaacttga atatgtttta tggcatctcc aaaggttaaa
     2221 gttaccgctc cggtatttgc aatatcactt gatataaata ctccatcttc aatttttcca
     2281 accctgtttc tataggaagc agtaatgtac ttccatggta tatatgcgga attgccgtct
     2341 ttatcaattc caagaaccgt gcctagttca tatttatcat taaatcctag ggttaatgaa
     2401 tcagtatcca acttcaatgc caccggcttg tctaatatat ctatctcaat atgtccttct
     2461 gcattcccgg catgggcggt aatagttgct tttcccggtt tttcaggcat gaatacatta
     2521 tttacaacct tacctgcacc ggcgggagaa acagtgtaat ctacattttt caaatcaaat
     2581 tctacaggag tataatattc attgtaaaac tttaaatcta aatttgtttc tgttttatta
     2641 aataccgtat tttgtgatgc tgttatttca attttatcaa ctttgttact atcaggataa
     2701 ttattaaata ctccgacacc cgatgctatt tttctttccc ttccgtctga cgggaagtta
     2761 acaacagtaa catttgcatt tcttaaaaag tcaacagcca tggttgtgga gcctcctcca
     2821 tccatattaa cagcattgta agcgcctaat tctatcatta tttttgcaag ttctgtctgt
     2881 tttgcaccca cataatctcg gtgcctcccg tcaacagtta ccaaaattat ttccgtattg
     2941 tctttgttaa aaccgattgc tgttcttgga tttactccta agacctgcga acttatcaca
     3001 ttttccctgc cgtctttaac caagtagttc agtgcaccaa aagaccaatc aatatcttcc
     3061 gggctaaaat ccaactcaat atttaaggat acgggatgac cttccttgaa tgaattaagc
     3121 attaattcca ttgtagcagg atttgagcta gcaactatat acccgttttc cggaataatc
     3181 gtagaaggct gattttttct tactgatgat acgatattat caactacaac aacttcaact
     3241 atatcctgtg ttgtgtatcc gggagatgtt ttattccagt cggatgtgag aattgtcgga
     3301 ccccattcaa tatttgaagt tttattcatt acaacaacgt tgaattccgt agaatccgag
     3361 ccataaaggg tgattttggg gttccatacg gaaatatcaa tttcatcttt taattttgaa
     3421 acggtgggat atccgtcatt aaaaggaaga ggagaagtta ttaatttatt gtctctgatt
     3481 attggaccgt atgtatatgt ggggtccccc atgtagaaaa agtctccgtt aacagctgca
     3541 accgctcccg atgacctaat catggagctt aaagttgatc tgtttgaaat gccgtcttca
     3601 ttgttaatag gtttaatttc tgtatgctca tcggttaaat ctgcccggat aacattaata
     3661 ttcatccacc ctgatgatgt atacttttca attctttcat atgtaatact ctttgagagt
     3721 ctaatttctt cagataagtc gtaaacagta taaagactgc tgccgtatgc tgaagatgat
     3781 aataatagta ttgctgcagc agagatggct gctattcttt taagcatcat gttaatcatt
     3841 cctttctttt ttaaattata acataagtgc aagacgaatg cgcttgcttc gcaccgatac
     3901 aatgagaaca ttaaattcaa aaagacttag tacaagtttc atcttgtatt cattttatca
     3961 gaattcaccc ataattgcca ttaatttaag gatattagag catgttaaaa tatagcaaat
     4021 cagggtttta tttatctatt atagacgtaa ttataataat atagttccat ataatcaata
     4081 ataatagtaa tacgataaaa atttaaacgt tgacaataaa aaacggatgt gataatatta
     4141 aaaaaaatcc ttatcaagag tggtggaggg acgaggccca atgaaacccg gcagcctgat
     4201 tattcaaggt gccaattccc gcggattttc cggaagatga ggctatagga aactatataa
     4261 cctcttcttg tgaagaggtt ttttagtgat aaattgttga tagcctatac ggccgttcaa
     4321 attttcgaga cgaatttatt gcattcatta cgtgcaaaat attaaaggta tgcaaaattt
     4381 aaatacaaaa aaggaaagga taaaataatg agaaaatggt tatttacatc tgaatctgtt
     4441 acagaaggtc atcccgataa aatgtgcgat cagattgcag atggcatctt agatgcaatt
     4501 atggaacaag atcctaatgg aagggttgcc tgcgaagcaa ttactaagac cggatttgta
     4561 cttgtagcag gagaaatcag cacgagctgt tatattgata ttccgcattt agtaagagaa
     4621 actataaaga atatcggtta tgacaatgcc ttatatggat ttgattataa aacatgtgct
     4681 gtatttacat caattgaaga gcaatctcag gacatagcat tgggtgttaa tgaagcagat
     4741 gaatataaaa agaataataa tgatataaat gatatgattg gagcagggga ccagggtatg
     4801 atgtttggtt ttgcctgcaa tgaaacacct gaattgatgc ccatgccgat atccttggca
     4861 cataagctgg caaagaagct tgcagaggta aggaaaaaca atatagtgga ttacttaaga
     4921 ccggacggaa aaactcaggt tactgttgaa tatcatgata ataaacctgt tagaattgac
     4981 acaatagtta tatctaccca gcataatcct gaagttaaca tagatacaat aaaaaaagac
     5041 cttattgaaa ttgtaataaa accaaccgtt cccaatgaat tgttggatga gaacacaaaa
     5101 tatatcgtaa atcccaccgg aagatttgtt ataggcggcc ctcagggaga ctcaggatta
     5161 acgggaagaa agataatagt agacacctac ggcggatatt caagacacgg aggaggagca
     5221 ttttcaggaa aggaccccac aaaggttgac cgttcagccg catatgcagc aagatatata
     5281 gctaaaaatg tagtcgcagc aggattggca gacaaatgtg aggtagaagt cgcttatgca
     5341 atcggagtag caaaaccggt ttcaataatg gttgaaacat tcggtacaaa caaggttgat
     5401 gatgaaatta tacaaaaagc aataaccgag aattttgatt taaggcctgc tgcaattatt
     5461 gaaaaattta atttaagaag acctatttat cagaagcttg cagcttatgg tcactttgga
     5521 agagaagacc ttgatttacc atgggaaaag acagataaaa cagatgagtt aaaaaaatat
     5581 ttataaaaga tatggggaca cttcttttga ggaatgtccc catattagga gtatatatgt
     5641 ttgaaagaag cacgggtatc ctattaccca ttttttcact tccatcaaaa tacggtatag
     5701 ggactcttgg aaaagaagct tataaattta ttgatttttt gcgctcagcc ggacaaaaat
     5761 actggcagat tttacctata ggccatactt catatgggga ttcgccatat tcttgttttt
     5821 ctgtatttgc aggaaatccg tattatatag acttggatgt attgtttgaa gaaggattca
     5881 ttgaagaaac cgacttaaaa ggtattgact tcggaagcga tgatgagcga atcgattata
     5941 agaaaatatt tgaaactaga tatggaattt taaaaaaggc ttatttaaat aaagggatgt
     6001 cctgtgattt agcatctttt acctgtaata atccatgggt ttcggattat gcattgttta
     6061 tgtctttaaa atataaaaat aatcaacttc cctggtataa atgggatgaa atgtttatta
     6121 aaagagatat aaataaactt caaaagtata aaaatgattt ttatgatgaa ataaactttt
     6181 ggattttttt acagtatata ttttttgagc aatattttaa gttgaaggat tatgcaaaca
     6241 gctcgggaat atcaataatc ggtgatattc ctatttatgt tgcagaggac agtgttgaag
     6301 catggacaga aaagaagctg ttaatgttaa atgaagataa tctgcctata tgcgttgcag
     6361 gtgttcctcc ggatgatttt tcagacgatg ggcagctttg gggaaatcct gtttataatt
     6421 gggagtactt aaaagaaaca ggatacatgt ggtggataga cagaattgca ttttcattta
     6481 aattgttcga tgttgtaagg attgaccatt tcagaggatt tgatgaattt tgggcagttc
     6541 cctacggctc tgaaaatgcc gtaaacggta gctggatgcc tgcttatgga aaacaaatgt
     6601 tccaaacatt aaagaaaagt ttgggtaatt taaatattat tgcagaagat ttgggaatta
     6661 ttaccgacag tgttattaaa ttaaaaaatg attcaggata tgcgggaatg aaagttttgc
     6721 aatttgcatt tgacgggaat aatgataacc cttatttgcc ttataactat gaggaaaatg
     6781 atgttgcata cactggtact catgataatg atacattaaa gggatggttt aacaaactta
     6841 cgcttaaaga aaaacaatat gttttacaaa aattaaatat taatatggtt aatgaaaaag
     6901 atgataacaa tttagtatac gagttgataa aagcaattta taaaagcaag gcatcccttt
     6961 gtatagtacc tttgcaggat tttctttgct taggctcgga ggcaagagtt aacataccgt
     7021 caacattggg caacaactgg agatggagac taaaaaactc aatgattaca gatgaattag
     7081 cagaaaaaat aaaagcttta aaaactgaaa gagatgttca aaatttcaat taatgtgtga
     7141 atttataatg agtttacctc aattatcgac gtattaatct aaatttagaa tatattcgac
     7201 ctgaaaaata tcagtaaaat agcatttcta tcaaaaaaaa cacacaaaac ttgaaatttt
     7261 gagcattcaa agtttacaga gctatatatt aataaaaaaa agaaccgtcc ataaaatatt
     7321 atgatagtaa aatcaagtaa ttttcctttt ctatattgaa aatattattt tttatattat
     7381 ttgtgtattc aatcatatct tctttgaatt ttaaagtaat ttctttttgt gataaattta
     7441 ctgctataat aatcctttca ttttcattaa atctttcgaa cacaaaaact tccttatcat
     7501 gaattaaaca tctgtattta ccttcagcaa aaattttatt tgttcttcgt aattttgtaa
     7561 taaataaata aaagtcaagt atctctttat tttctttccc ccaaggatag catcttctat
     7621 tgaaagggtc atcccatcct tcaattcctg cttcgtcgcc gtaataaatg cagggaatac
     7681 cgggtagagt catttgcagc aagactgcaa tttttaaaaa cttaattcct tgctccaatt
     7741 cctcgtatga aagtttgctt tctgccattt cgcttctgct tgaaggtact cttttgcttc
     7801 ccaatattgt cagtattctt tcagtgtcat gagttcccag cacattcatt aggcagttta
     7861 cggtttcagg aggatatgtt tcaataactt tattcattat atgattaaga ttagtgcagt
     7921 ctttattttt tacaaattcg attattcctt ttcgcagggg ataatttgtt acagaatcca
     7981 attgctttcc gcaaagatac tctctcagtt tgttgtatga atacttattt gaagcatctt
     8041 cccatacttc accgattatc agtgcatctt cattattctt ttttattgaa tttctaagag
     8101 caataatgaa ttcatccggg agttcgtcag caacatccaa acgccatccc ataatgcctg
     8161 ttttttgcca gtgggtgata acaccgtttt tccctgttat gaaatttata taagagtcat
     8221 tgttttcatt cacttcaggc aatgttttta taccccacca gctcacataa tcatcattcc
     8281 aattattgaa tgtataccaa tcgtaataag gtgaatcctt gctttggtaa gcacctatgg
     8341 agtcgtacct gccatattta ttgaaataaa tgctgtcatc gcctgtgtgg ctgaaaactc
     8401 cgtcaagaat aacatgtata ttatatttct ttgcttcttt tataagtttt ttcaaggagt
     8461 tttcagtgcc gaacatgggg tctatcttat gataatttcc cgtatcgtac ttgtggtttg
     8521 aatatgcttc aaagattggc gataaataaa ttatgctaac acctaatttt gctatgtagg
     8581 gcagtttttc tctgataccg tctaaatttc ctccaaagaa gtcgttattt aaaatctcac
     8641 cctttttatc aggaagatag tatggagttt caccccaatt cctgattatt gctccttttt
     8701 ttttgattat tttagtcctg aaaaacctat ccacaaaaat atgatatata attcctccct
     8761 tagcccaatt tggagttata tatttatcat catatatcag cagttcatag tctttggatt
     8821 tttcattatc aaaaataaag gaataatagt aatgaccata ttcctctatg gaaagcatag
     8881 cttggtagat atcatagttc ttttcttgtg atatccaatg catttttatt ttatcgtgaa
     8941 aagtcctact gtaatcatgc cttttttcaa tcaataattc gggtgcacaa atgtttcgct
     9001 ttacaagtat tttaaatgta attatttcat ttgatttaat tccgccttga gggtatctgt
     9061 aaaattcaga ttgcgaatta aatttataca taatgcctcc ttgttgtaat taatctgggg
     9121 acattcctcc tactccaata tcctcataat agagttgtgt ccccacacat taaactaccg
     9181 gtttaatacc ccatatattg tcggcatatt ctcgtattgt tctgtcggaa gaaaaaacac
     9241 ctgctgatgc tatattcatt aatgacattt gattccactt ctttttatta ttgaagtaca
     9301 gctgggatgc tttttggtgg acttccatat agctgtcaaa gtctgcaaaa cacatgtaag
     9361 ggtcatccac gacaggtcct cctatcaagt atctggatat ggattcaaag gattttcctg
     9421 aaatcccttt gtttaaaaaa tcaataattt ttctgagcat gagacttttt tgataatata
     9481 ttactgatga atatccgttt ttccacaatt tttcaacttc atagtctttc attccgaaca
     9541 aaaagaaatt atcttctcca acttcattaa ttatttcaac attagcaccg tcataagttc
     9601 ctatggttat tgctccgtta agcataaatt tcatatttcc cgttccgctt gcttcttttc
     9661 ctgccaaaga tatctgttcg cttatttcac cggcaggaat gattatttca gccaggctga
     9721 ctgaataatt ctctaagaaa accacattaa tatatttttt tataaaggtg tttttgttta
     9781 tatattcgcc taatgcataa ataagctgaa ttatttcctt tgccctgtgg tagccgggag
     9841 cagcttttgc agcaaatata aatgtttccg gcactacatg tgaagtattg ccttcattta
     9901 tatcgattat gatgcttata attctaagag catttaacag ctgccttttg tattcatgca
     9961 gtcttttcgc ctgtatgtca aaaatcgaat ctatgtttaa atcgatatga ttattagttt
    10021 tcacatattg tgcaagcctt tttttatttg tatatttgat ttcatcaagt ctgtttagca
    10081 cactgtcgtc atccttataa tgttttaaca tattaagtct ttccgggctt gttctatatc
    10141 cgggtccgac aagatcatca agcagagctc caagtggagg atttgcctga ttaagccatc
    10201 gcctgtgagt tataccgttt gttatgctta taaacttatc aggtgtgtat tcataaaagt
    10261 ttctgaaaac agttcttttt aatatttccg aatgcagtct tgctacgccg ttcactttgt
    10321 gactgcctat tatacacaaa ttagccatat ttactttgcc atagccgaat atggacatat
    10381 taaaagcttt gtcccggtta ttgtatttag ctgatgcttc ttctatgaat cgcctgttta
    10441 tttccttaat tatcatatat attctcggaa gcttagtttg aacgatatta ttatcgaatg
    10501 tttccagtgc ttccttcaat accgtgtgat ttgtgtagga taccgtatta actacaatcg
    10561 tccatgcttt ttcccacgat aatccgtatt catccataaa aatacgcatt agctcaggaa
    10621 ttatcaatga agggtgggta tcatttatat gaattgcatt tttttcactg aagttatcca
    10681 atgaattgta ttttttcata tgatttttaa caatattttg tgctgatgca gaaaccaaaa
    10741 aatattgctg cttaagtctt aatattttac cctcattatg gtcatcggca ggatataata
    10801 cttttgatat tgcttctgcc attgagtctt cagataaggc ccttatatag tctccctgcg
    10861 aaaacaaatt catatcaata ctgtttatac ttcgggattc ccaaagcctt aaaaggctga
    10921 cacctttgca gttataaccg gaaatcatca tatcataagg tattgcttct atttccgtag
    10981 ggcaatcatg atgtatctta agtccttcgc cggtccattc ttctcttatt gtgccgtcaa
    11041 actttacaat gactctttcg tctgtcctgg gcataagcca tacatctgaa gtagtcagcc
    11101 agtcatcggg aagctccgtc tgccatccgt caattaactt ttgtttaaaa agtccgaatt
    11161 catacttaat tgaaaaccct actgccgcat agtcttgtgt tgcaagagag tccatgaagc
    11221 aggcagcaag ccttccaagt cctccgttgc ctaagcccgg atctctttct attgaatata
    11281 gttcttcggg agtaatgtta tacttcttca gagcttcttt cgcctcatct tcaataccta
    11341 aattaaacag gttattgcgt agagattttc cgataagaaa ttccatgcat atatagtata
    11401 cttgcttatg ttcattctgc attcttttga attcatttct tttttttaag agcaaatccc
    11461 ttattacaat tgaaaatgcc ctgtaaattt gatttatagt cgccttctca ggctcagtat
    11521 tccaatatcg aataatttct tcatctatta aacggttaat cagtgttgca tcgattttag
    11581 aatccttcat tattattctc ctcaaaattt gccagactgc tatacaagtc aaaatatttt
    11641 tctgcagatt ttctccagct gaaatccatt ttcataactg tttcaactaa ttttctccaa
    11701 tcctcttttt tttcataaac tattagagcc cttttgacag cttcgcacaa tgcttgaggg
    11761 gtttcatcaa cgaatgtaaa accattgcct tttccaaggc ttgcatcctt tatcgaatcc
    11821 ttaagtcctc cggtctctct aacaatcgga actgttccgt aacgggaaga aatcatttga
    11881 gcaataccgc aaggctcact tattgaaggc atcagaaata aatctgcacc tgaataaatc
    11941 ttttttgcaa aatcagaatc aaagccgatt cttacgtata ttttatcacg ataactttct
    12001 tgaaggtatt taaagaataa ttcatatccc ctgtctccct ttccgagtat gataaactga
    12061 accttttcct ttagtatttc ttccatggat tcttttatca ggtcaaatcc cttatgctta
    12121 accagtcttg atataatccc tattacggga atgtcttcat cctgcggcag ccctgataat
    12181 gattgtaaat ttattttgtt taaatatttc ttttgaatac tttctgcatt gtaattttca
    12241 aatatttctg tatcgtcttc aggattgtat ttttcggtat ctattccgtt taatattccc
    12301 ttaatcttgg atgaattttt ttgtataatt tctgccagcc cgtgagcata ataatcatct
    12361 aatatttcct ttgaatagct ttcgcttacc gtactaataa aatgagcggt ttccaaggca
    12421 cctttcaaaa ggtttattgc tcctttaaat tcaattatac ctttttcctt tccgtgcaag
    12481 tcaaatacat cctccgaaat agctaaatca aacacccctt gatattcaat gttatgtatt
    12541 gttattattg attttatatc tctgtaaccc tcataaccgg aatattttgc agataaataa
    12601 atgggaatca atgatgtctg ccagtcattt gcatgaataa tgtcaggcat aaattttatc
    12661 ataggcagta tatacaatac cgccttagag aaaaatgcaa atctttcggc atcatcaaaa
    12721 tgaccgtagc attcactcct tttaaaatag tattcattgt ctataaaata atatgtaaca
    12781 ttatttaata tggttttata aacgccgcaa tattgctgtc tccaggcaag aggaacggat
    12841 gttttcccaa taaattcaaa ttcatttctg tccacaattc cttggtacaa aggaagtact
    12901 atccttatat ctattttgtc cttatatttc tcgaccaatg ctttcggcaa tgacccggca
    12961 acttcaccaa gtccgccggt tgctataaag ggtgcagctt cggatgcaac atataatact
    13021 tttaatttat tcatataatc actcctatac aactgagttt ttctttataa agaagggaca
    13081 gttagaatat cccaacaaaa ccctatcctt gttcacaaaa gtgtatgtgt cggttattat
    13141 gtagtccaat ttggcattgt cgcttattct tccatgctgc ataataattg aatttttaac
    13201 aactgcattc ttgcctactc tcacatctct gaaaagtatg gaattttcga cagttccttc
    13261 aatgatacag ccgttggcaa tcaaagaatt tttaacaact gcatctttac aatacttagc
    13321 aggagccgta tcttttgttt ttgtatatat gggacctgta tttgaataaa atatattttg
    13381 caatgtatcc atatttaaaa gtttcatact gttttcataa tagctttgca aagagtcaat
    13441 atgtgcaaaa aatccggtat attcatatcc gtaaattctt aaagaatgaa ccctggcaga
    13501 taaaacatca aagctaaaat ccttgtaatt tcttgcaata gcatcataaa taagggattc
    13561 caagaattcc ttgttcataa cccatgtatt taatcctata ttgtaatttc cgcttgtaac
    13621 cggataaact aagatatcat cagctcttcc atcttcatca aattcatata atattttatg
    13681 aattgaatca gatttattta tgtctgtctt aatatagacg gcagtaatat ccgaattttt
    13741 ttcttcatgg tattgtatta ccttattata attaatatca taaatcatat cggagtctga
    13801 taatacaaca taatttcttt ttgaaaattt gatataatcc aaaactcttt tcaaggcatc
    13861 gagtcttccc ttataaagcc cgtggttttc tcctgcagaa taggggggaa tcataagtat
    13921 gccgcccttc tttcttgaca agtcccaatc tttaccggaa cctacatgtt ccattagaga
    13981 gcggtagttg ctgcttgtta tcaggtatat tttatttatg ccggaattaa ccatatttga
    14041 gagcgggaaa tctataagcc tgtacctgcc cccaaaaggt attgcggcaa ctgagcgatg
    14101 agatgctaat tctgtcatgc tccaatcttg catatctgag aaaataattc ctaacgcatt
    14161 catattattc ctcctgcatt ttatttataa cactttcacg cggtattacg gtaatttcag
    14221 agttctcccc ctttggtttg ccaattttta agttggctcc gattgttacc atttcatcta
    14281 ttatcgaata attaattata caatttttat ttatttttac atctgccatt atcacggaat
    14341 cttttacaag agcattctct tctataacta ctcctgaaaa tattactgaa ttctctacat
    14401 ttccaagaat tactgcacct tccgatacta atgaattatt aataagagca ttctttccta
    14461 catagtgggg gggcttttct gcatgccttg aatatattat ccaatcttct gataattcaa
    14521 agccattctc gcccaaaaga tccatatttg cctgccatag tgaatctatg gtaccgacat
    14581 ccttccaata tcccttaaat ttgtaagcat acaacttttc atttgatctt agcattagag
    14641 gaataacatc tttcccgaag tcatttgatg agtcgggatt tttatcatct atttcaagat
    14701 actttttaag cttgttccat ttaaatacat atatacccat agaggcttga gtactttttg
    14761 gcctttttgg tttctcttca aaatcaatta ttcttccgct gtcatccgag cttaaaatcc
    14821 cgaagcggga agcttccttt attggaacat caattaccga tatggtgcaa tcggcgtcca
    14881 ttctcttatg gaagtcaatc attaaggaat agtccatttt ataaatatgg tcaccggata
    14941 aaataagcac atattcagga gagtactggt ctatgaaatt cgtgttttga aaaattgaat
    15001 tagctgtacc caagtaccat tccgaatccc ctactctttg ataaggaggc agtatttgaa
    15061 ttcctccgta cattctgtct aaatcccaag actttccgtc acctatatac gaatttaata
    15121 ttaatggttg atattgtgtc aaaacaccta ttgtatcgag gccggagtta acacaattag
    15181 atagggcaaa gtcaattatt ctgtatttgc ctccaaacgg tacagaaggt tttgcaattt
    15241 ttgttgttaa ggctcccaat ctgctgccct gacctccggc aagcagcatg acaacacatt
    15301 cttttttagc taatttcata aattttccct ccctctttgt taactttata tttagtttct
    15361 ttttctaaaa agattatcga aaggggcggc agggtcaatg atacaaaaga attatatccg
    15421 tgtaccgaac cttgagatga tattaatcta tccttatttc cggtgcctgt ccctccaaat
    15481 tcctcatcat tggaattaaa tacttcagtg taaataccct catcaacgcc aattatatag
    15541 ttatgtcttt ctgtaggtga aaaattaacc accgcaatca attcatcacc attcttattt
    15601 attcgcttaa atgatataat attttgctca taatcatcat ttgaaatcca tttaaagccg
    15661 ctccatgaat aatcaacttc ccatagctca gaacgtttca gataaaactc gtttaaattt
    15721 ttaatatatg tatttaattt tccatggtat tcatactcaa tcataaacca ttcaatttcc
    15781 ttgttataat cccattccgt aaattgtcct atttcatatc ccatgaagtt aagttttttc
    15841 cccggatgag ccatcatgta gcccaaaagc accctgacat ttgcaaactt ttcctcataa
    15901 ggtccgggca ttttattgag tatggatttc ttgccatgaa caacttcatc atgagacaaa
    15961 ggaagtatat agttttcact gaaggcataa tgaagtgaaa aggttatgtt tttatgcttg
    16021 tattttctga aaaacgggtc agtttccata tattctatca tatcgttcat ccagcccata
    16081 ttccacttga aattaaaacc cagtccgcct aaataagtgg gttttgtaac cattggccag
    16141 gctgtggatt cttctgctac catcatggta ttagggtaat ttttaaatac ggattcatta
    16201 agctttttta aaaaagaaat tgcttcaaga ttttcattcc ctccatatat attgggagtc
    16261 cactcacctt cctttttatc ataatccaaa tataacattg agcttaccgc atcaactctc
    16321 aagccgtcgg catgataaac atccaaccag aatacggcgt ttgatattaa aaatgattga
    16381 acttcagttc tcccgaaatc aaatttatga gttccccagc ctttaatttc tcttttttca
    16441 ctatccttgt actcgtaaag aaaatctccg tcgaatttat acagtccgtg ttcatccttg
    16501 gggaaatgag caggaaccca atcaagaata acacctatcc cattttgatg gcattggtca
    16561 atgaagtatt ttaaatcatc cggctcaccg agacgcctgg ttggagaata gtaacctgaa
    16621 acctggtagc cccaggaacc gtcaaaggga tattccataa tgggcataag ctctatatgg
    16681 gtatagtaat gttttttaac atggtttatc aatagcggcg ccatttctct gtatgtataa
    16741 aaatccccat tttctttacg cttccaagag ggtagattaa cttcgtaaat atttatggga
    16801 atttcatata agttaattct ttttttatat tcaaaccaat ttgtatcatt ccatttgtat
    16861 ttgttaatat tataaacaat tgatgccgtt tccgtcttag tttgtgaaga aaatgcatat
    16921 ggatctgcct tatatataat tgtattatcc gcagcagtaa ttatatattt atacaattca
    16981 ccttccttta tgccgggagc aaacaattcc catatgccgc tatcagaaat ttttttcatt
    17041 tcatactcgg cacttttttg ccaattgcaa aaattgcctg taatgtgcac attttttgca
    17101 ttgggtgccc atactctgaa aacggtgcct atgttttcta taatatgcga gcctaaaaaa
    17161 ttatatgaat aatagtttgt accttgatgg aataaataaa taggtaaatc attctttttc
    17221 ataaaagccc cggtaaaatt tatttgttct tatttaaagt atacgacaaa ataaataact
    17281 tttaaagata taatttataa acataagtac ttataatata agtatacctt gctttagaca
    17341 agtttaaaca aaaaagacga caaaaaaaga acactcatat gaatgccctt ttatctaata
    17401 atcaattaaa ttctattttg cctacacctg tggtaagatt aatgctggta ccttggtcat
    17461 gttttgtttg gattctttca tctttcataa attcattgat aaccgcatga tatcccgagt
    17521 cgtcatttag cgatacttca atatttccaa ctcctgtttc tgctataatg ctcactgctt
    17581 ttgaaatgct ttcaaaggac aattctatat ctccggtact tgttttaaat tcagaatttc
    17641 ctactgctat gcagtctttg agaataattc ccccaacgtc ggcttttata ttatatgaac
    17701 catttgattt atctaaaata acttcaccaa cattattttt tatatccatg ttcccgataa
    17761 aactgtttat gtttacatca ccgacatttg ctgtaacttc taaattttgt atggtagaag
    17821 gaagatatac ggttaaatcg gttacaaggt ttatatcatt agggagctca ttaaatgttg
    17881 tatcaataac tatggagctt ccttcttcat taattttgta aatgtaatta tcgataattt
    17941 cttcagcttt tttcttttct tttgcttttg ctgtaatatt tatatttatt attgtatcat
    18001 cagattggtg cggtactata tttatatctc caacagagct cattaagttt agagtatttt
    18061 tattttctga tttaacagtg tgagattcct ttctttctgc tttttcattt cccgcaccgt
    18121 taaatactgt gtctgtaata atgtcggaga catccttaac tatattttca tcagccatat
    18181 ctaatgaagt gcagcctgta gacagaatca tagctattaa taatatacta attaatcttc
    18241 tcatatttcc ccctgtttat aagttaattt tcttttctaa aagatatcct gaaattcctg
    18301 ttaaagaaac agcctgaatt attaatacgg caatcattgg aagcaatatc atagctaatc
    18361 ctccggctga aactctgcct gtacttgata tagtatcaac ggtatcataa tagggagtaa
    18421 taatatcata gaatttactt gataaaaatg aaagtaccca attaatcaat aaaaatatta
    18481 tgaaactaaa cagcccccca aatttaattt cggaaaatat actttttctt attgtcatag
    18541 ctgtataaac cgttgtaata aatgctatta aaaatgcaag tgcagccagc aaaaatacta
    18601 atacgtggcc caagttgaaa ccaagactgc cggacaataa attgtcaaca gcttttatta
    18661 tttgactgta atcaatttca ttacccgctg aaataataat atatgttccg tttatcagta
    18721 taaatataaa atatattaat actagtgcaa agccttccag tatagctgct aagagtttcg
    18781 atgatatgat tttataaccg ctgttaggtg tcataaacag catgtaacct gttttcttat
    18841 taaggtcatc gctgtacatt tttataatgt ctgccaagta gaaaacatat aaaactatgg
    18901 gagataacac aagaaataac atgcttccgc ctactcctct tgtcagtaag aacaaattca
    18961 atgctagcat tgtaatcagt atcatagaaa ccaatctata cttcctaata aactcatact
    19021 taattaaatt aagcatttct gaatacctcc ttatatattc cttcaaccga caattgcctt
    19081 tcctgcctta attcttcagc atcgccgaaa acctctacaa taccttcttt taagaaaata
    19141 acggtatcaa gtattttttc aatttcactg actaaatgtg ttgaaataat aattatttta
    19201 ttttcactgt gagattttgc aattaaatcc aaaatggtat cccttgataa aacatctaca
    19261 ccgttcaaag gttcgtcaag catgtatatt tgtgcatctc tcgataatgc aataaccgtc
    19321 ttaagctttc cgatttgacc cgatgacaaa tttgaaattt taatgtctga atttacgtct
    19381 atatcattta aaagattaac ggcataatca tacctgaaat cttcatacat tgtattgtag
    19441 tattttaata tatcaatgac tttaaaattt tcatacaaat agttttctgt aggcatgtat
    19501 gaaacatatg ccttggactt gtaggataac atttcgtcat ttattaatac actgccgctt
    19561 gtttgcttat gaagacctgc cacaactttc ataagagtgg ttttgccgct tccgtttggc
    19621 ccaagtaagc cgtaaatttt accttgctca aaatccaagc tgacattttc cagtgctgtt
    19681 ttattgtaat atctttttgt taaatcagta atttttaaca cactcaattt attcattccc
    19741 ttctaaataa ttattttctg tttcgataaa tttaatcata tcttccttgg tgtatcctaa
    19801 ttgtttcatt ccatcaataa atgcagtgat ttgattttta gccattgtgt atcttaattt
    19861 ttcaattatt tggtcagatt ctgttatgaa tgttcccaaa cctctttttg taaatactat
    19921 ttcctcattt tccaactccc tatatactcg tgcaaccgta ttgaaattta tacctaattc
    19981 attggagtag tctcttgaag aaggcatttt ttctccgggc tttagtttcc ctgaaactat
    20041 atcttgttta atcttttcta ttacttgtag atatataggt atattgttat tgaattccaa
    20101 cttatcacct tcttttgtat attagtgtca tagtagaata gtatactaat acttgagtta
    20161 tgtcaatagt ttaatgttaa catttcaaac aataattagt tacaatttcg taacaattgc
    20221 ttttaattat aacttgacat aaaattgata aagttatata aatggataga gaaattgagt
    20281 tattacatag agcggaggga acaatgcata aaaaattatt tattcctgga cccgtagaag
    20341 taaaggaaga tgtgctcagt aaaatggcta ctccactgat tggtcatagg agcgaggatg
    20401 cttctaatct acaaagagat atttcaaata atcttaaaaa attattttat acagaaaatg
    20461 aaatattatt gtcaacatct tcaggtacag gattgatgga ggcagcggtg cgatgctgca
    20521 ctaaagagag ggctgctatg ttttcaatag gctccttcgg cgacagatgg catgaaatgg
    20581 ctgtttcaaa cggtatcaat gccgatttat tcaaatcaga attaggaagt attacaagac
    20641 ctgaaatggt tgatgatgta ttaaaaacag gaaagtatga tttagttaca ataactcaca
    20701 atgaaacatc aacgggagta atgaatccta ttgataaaat aagtaatgta ataaaaaaat
    20761 atccggatgt aattttctgt gttgatgcag taagctctgc aggaggagta aaaattgaga
    20821 cagataaact tggaatagat atctgcataa catcatcaca aaaagcattg gggctgcccc
    20881 cgggactctc tatgtgcacc atatccgaga gggctgtgga aaaagcaaga tatataaaaa
    20941 acagggggta ttacttagat ttggttcaat tgtatgatac aatcaaaaaa aaggattatc
    21001 agtatccgtc gacaccaaat ttatcattga tgtttgcttt gaattatcaa ttaaaactga
    21061 taatgcagga ggggcttgat aacaggttta acagacattt cgccatggct gaatacacaa
    21121 gaagctgggc aaaaaagaat tttgcattat tcgcggatga aaaatatgct tccaatacgc
    21181 ttaccacagt gaaaaacatt cgaaatataa gcgtaaagga tttaaacatt gaattaggga
    21241 aaagaggttt tgctatttct aacggctatg gcagtttaaa agaaaaaaca ttcagaattg
    21301 cacacatggc ggacttaact ttagaagatg taaaagaact tattttaaat atagaagata
    21361 ttttatcaat ttcttaaaca tataatatcg ggggatttaa atgtacgatt taaacaaaat
    21421 cagcagttat ttaaatacaa atacaattgg ccagtctatt attcaatacg acaatttgaa
    21481 ttctacgttt gcaaaagcca aaaatatatt ttcaacatgt cctgacggaa ctgttgtatt
    21541 atcggaaaac caatccgaat gtattttaag gtttggcaac gaatggcttt gctcacctga
    21601 taaaaatatt tatttaagta ttattttaaa atctattaat aataattatc tggtaccctt
    21661 aacagatgtg gttgcttgtt cttctatatt gaaatccata gaggagctgt acaacttgga
    21721 ttgcagaata aaatggccta atgatatttt tataaataat aacaaaatat cgtctgtaaa
    21781 aagtgaaatt ataggcaagg gtgcaggtat aatattatcc ttatatataa atgttaatat
    21841 ggaagaaaaa attgaaaatt taaaagcgac atcaataaaa atcgaaatta ataatgatgt
    21901 tgaaagagaa cttcttatag gcacaatatt aaacaacatt gaatatcatt atgttgaaat
    21961 gataaacacc ggtaaggctt atcattccgt tgacatatat aatgaaaact tgctgtttaa
    22021 taataaggta gtcggagtta tcaaaagggg cagaaaaacg gttagaaaag ctgttgctaa
    22081 gaatattgac agcgatggat ggcttcaagt aatcaatgag aaaggcgaag aagaaatatt
    22141 agatccggga gaaactataa tacaatatga agaaacttaa aattggaagt attgaattag
    22201 atacaaatgt tgtgcttgca cccatggcag gcataacaga tataacatat agaactattt
    22261 gcaaagaaat gggagcgggt cttgttcata ccgaaatgat aagtgcaaaa ggattgtact
    22321 atcaagacag aaaaacagaa gagcttatga aaataaacaa aaacaacaga cctgtgtcaa
    22381 tgcagatttt tggaagcgac gctgacataa tgtcgtatgt tgtcgaaaaa tatatcaata
    22441 gcagaaatgc tgttgatatt atagatgtta acatgggctg tcctgcacct aaaatagtta
    22501 aaaacaaaga cggctgtttc cttatgaaag aacctgagtt ggcaggtaaa ataataaaca
    22561 aaataaaacg cacaacaaaa aaacctgttt cagctaaaat aaggtcgggc tggagtaaag
    22621 attccatcaa tgctgtagaa tttgcaaaga ttttggaatt taacggttta gacatggtga
    22681 ctgttcacgg cagaaccaga gagcagtatt attcaggtaa ggccgattat agaataataa
    22741 atgaagtaaa aaaaagcgtt aatattcctg tcgtaggtaa cggtgatata ttcagtcctc
    22801 atgatgcagt taaaatgatt gaagaaacaa attgcgatgc agtcatgata ggaagaggtg
    22861 ttttgggaaa tccgtggctg ataatgaata cagtaaaaat tctaaacaat gatatgaatt
    22921 tttatacacc atcaccattg gataaaatta atatgattaa gaggcatgca tccatgttgg
    22981 cagatgaatt aaacgaaaag gttgctattt tggaaatgcg taaatttgcc gcatggtata
    23041 tgaagggcat gaaaaattct tcacaaatta gaaacatcat aaataaaata accagtgcag
    23101 aggaattata caaaattttg gataagtaca ttgaagttta ttgcttaaat gaccttaacg
    23161 gtttaaatta acaaattaac ttgacaataa tcgtttactt gtttat
//
LOCUS       DAOSHP010000020        23071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1775_length_23071_cov_8.792970, whole genome shotgun sequence.
ACCESSION   DAOSHP010000020 DAOSHP010000000
VERSION     DAOSHP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23071
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1775_length_23071_cov_8.792970"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLB33_03420"
     CDS             <1..506
                     /locus_tag="PLB33_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62602.1"
                     /translation="LSYAVNVFILLVIISLILKLDFSKVSGEPVSYQDSEVKTESYVY
                     ALEKRMEEILSKIDDINSVDVMIYTKQTPILEPVYDENISNETNIESMSDGTKREVNR
                     ETKQNKVILGRDNSVVERYYQYPEISGVLVVVNYSGNKDIYSILINSVKTLLDIKLND
                     IEIIVIN"
     gene            516..1163
                     /locus_tag="PLB33_03425"
     CDS             516..1163
                     /locus_tag="PLB33_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOG62603.1"
                     /translation="MIMRKETLVFLASLAIIFIIGYINMSLTPDNAFDDLETGQMEPA
                     DLEGDIAEIIEGGVNEVDFAEVLPEETDYEILGDITDLSSAEEASSEGVLGVLNASFA
                     NFKLNKEKSNMDVLDYLEESLSSSLISDETKEQFEQLLLKKNSFIEAEQGIELMLQSK
                     GYNESVAIVDESMVKIVTNDTIEKADATVILDVVVSETNYTPSQIKIVKFDNIDL"
     gene            1206..1610
                     /locus_tag="PLB33_03430"
     CDS             1206..1610
                     /locus_tag="PLB33_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOG62604.1"
                     /translation="MDNEKFEFGQVKISDDVVIIIAGIAASQVKGVNTTRTGVTDGIT
                     NLFSKNNYSKGIKVDVNEDTVVVDIFINVEYGYKINEVAREVQLAIKKEIETMTDLKV
                     AAVNVHVLNIVQEKIQEKVQEKEKDAANIAIE"
     gene            1709..2113
                     /gene="nusB"
                     /locus_tag="PLB33_03435"
     CDS             1709..2113
                     /gene="nusB"
                     /locus_tag="PLB33_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOG62605.1"
                     /translation="MKRKETREEAVKIAYSMDVNKDFNKNNITEYLEHFELENMDIDY
                     LNKTISDMIDNMEEIDKHITDNSKDWKLSRIAKVDLAVLRIALSEILYNETIPESVSI
                     NEAVEISKKFSKEDSHKFINGILGTVVRCIKK"
     gene            2110..3057
                     /locus_tag="PLB33_03440"
     CDS             2110..3057
                     /locus_tag="PLB33_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62606.1"
                     /translation="MRYILGIDTSSYTTSVALINEESNEILADERKVLEVKKGQKGLR
                     QQEAVFQHLKNLPELYEKIPYDLSNIKIVSVSSKPRNAENSYMPVFIAGRNFGKVISD
                     TLNCEFLEYSHQENHIAASIINNYKNIDEFLAVHISGGTTEFLSCNKAIKGFDIKIVG
                     GTKDITFGQLIDRLGIMMGFIFPCGKYMEEYIKDKTFEKITRPRLIKGSYINLSGMEN
                     YFGNLLNKYSKEVIITSLFDYIAEYIVHIISSLSHLQHKTLIISGGVASNSYIRNYIT
                     KSVKGYEVLFPAVNNSSDNALGLAYLPIIDRWHNEIKTN"
     gene            3038..4219
                     /gene="xseA"
                     /locus_tag="PLB33_03445"
     CDS             3038..4219
                     /gene="xseA"
                     /locus_tag="PLB33_03445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOG62607.1"
                     /translation="MKLKPIKVSVLNQYIKKYLMSNSILNNLRVEGEITNIRISKTGY
                     TYFSLCDEISCISCVAFFADTISKNGDKIIAEGELSVYEAKGTYQLIVRDIERAGLGK
                     ILLDLELLKEKLKEQGLFDRKRELPEFPQRIGIITSKTGAAIKDILKTFENVKANIDI
                     TIYNTLVQGEKSKKSIIEGLKYFNSVETDVILISRGGGSFEDLNVFNDMDVAMEVYKS
                     NIVVVTGIGHETDRTLTDYVADVYCHTPTAAAERIIRNYKDIEEKLDNLVFTLKSETN
                     NTISLKKAELISNKYLLRSSLPLEDIYKTKNQLDTYKNLIKNKTTSQINEYYFNLKIM
                     LEKLNTFDYKKQLEKGFALVSDNSGNIIKHKEQVELKDILHIRFADFKIEAETRDIKE
                     V"
     gene            4221..4415
                     /gene="xseB"
                     /locus_tag="PLB33_03450"
     CDS             4221..4415
                     /gene="xseB"
                     /locus_tag="PLB33_03450"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOG62608.1"
                     /translation="MEYDNFESALEELKNIVRKFENKDDISIDELLENYEKGMKAYSF
                     CMKKLDDTQRKIKIIDENIE"
     gene            4613..5398
                     /locus_tag="PLB33_03455"
     CDS             4613..5398
                     /locus_tag="PLB33_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOG62609.1"
                     /translation="MKNYRLDVFLHNKGYVGSREKAKELILNNSVFVDDKLITKPSYM
                     VNDDVKIKITEDVYISRGYLKIEKAIHVFNINIENKTAVDIGASTGGFTDYLLKHGAK
                     KVYAIDVGCGQLHDSLKNDIRVVNLENTNFRYIDTSIITDIIDIITVDVSFISLQHII
                     PKIVELSHNDTDIVALVKPQFEAGKVNIGKKGIVKKREVHLGVLNSINNYCKTNNLKL
                     INMDYSPIRGGAGNIEYLAHLKITGNTNEINFKDLINKAFEKL"
     gene            5418..5870
                     /locus_tag="PLB33_03460"
     CDS             5418..5870
                     /locus_tag="PLB33_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOG62610.1"
                     /translation="MKKYTRQTKILELISKSEIETQEELAEALKNMGIDVTQATISRD
                     IKELRLVKVMSKSGKYKYATISQSQEGITDRLNKIFENSVVSIDNAMNMIIIKTIPGA
                     AQICASAIDFMGIEEIVGTLAGDDNVFVAVRKIEQVDKVLQEFKKNIR"
     gene            5881..7539
                     /gene="recN"
                     /locus_tag="PLB33_03465"
     CDS             5881..7539
                     /gene="recN"
                     /locus_tag="PLB33_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOG62611.1"
                     /translation="MLLSLNISNFAVFENVSVDFDSGFNVFTGETGSGKSVLINAIEL
                     ILGDRASKELIRKGKNSALIEGIFDIGDNDSLYEDLKSLNIEIDKDDFLIISRELLQN
                     GKSINKVNGRTYPLNTIKAIGSLLIDIYGQFGHESLFKKENHIKMLDSLIQNELSPLL
                     QSYNELFVKYNRVINEISTLKEKLLNKDKKIEQLQYEINEIDSAELKEFEEEELLKNI
                     KKLSNIQEIKKSLYDAIQILNSDNINNVYSIINKIKNYDEKLEEFLSRVDIQLEELKL
                     LSYDLRDYSENLDLDEKKLDNIENRMSLINRLKRKYGFSIEKINEYRNASHEELSLLL
                     KADSKLNSLITEKNEIYNLLIEKAGIISKKRKTAAEYLEKQILIELSELNMKNIEFEV
                     KINKKNISADGVDDVEFLISTNVGSDLNSVQKIVSGGEASRIMLAFKKIKSDIGQTMV
                     FDEIDMGISGKTAQMAGVKMNYIAKNNQVICVTHSPQIASISKNHFLIEKKVVDNNTF
                     STVKKLDKENKIYEIARLLSGMKITEKSINNAKELIEFNSKISE"
     gene            7590..8666
                     /gene="spoIVB"
                     /locus_tag="PLB33_03470"
     CDS             7590..8666
                     /gene="spoIVB"
                     /locus_tag="PLB33_03470"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOG62612.1"
                     /translation="MRNFLRRVLFFTFFIAVLMTAVFFGFNLKTNPYKFAKGDQVIDT
                     EGKYIIPGGQTIGIELKTEGVLVVGLADIINIDNISTSPAKIAGIEIGDKILEIDSIY
                     MRSTDEILNYTETMGIKVYTFKIERAGEIYNLTITPVQRYDSDEIKFGFWARDDIAGI
                     GTVTFVDPETGFYSAIGHGISDSETGKLIDIESGTISKANITNIKLGKKGEPGEIIGY
                     ILKDKESLGTVTDNTDFGIYGKINKDSMGYFSGDLMEIGKKEEIKLGPAYIISCVNNE
                     IKKYSVDITKIFYQNKPDEKSFVIKITDKELLEMTNGIIQGMSGSPVIQNNKIVGAVT
                     HVFMNDPTKGYGIYIEWIIDKIYD"
     gene            8885..9679
                     /gene="spo0A"
                     /locus_tag="PLB33_03475"
     CDS             8885..9679
                     /gene="spo0A"
                     /locus_tag="PLB33_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOG62613.1"
                     /translation="MQKKVKVVIADDNKEFRLILKDFLLSKKVFDVVDMAEDGIKTLE
                     SVEKHEPDILILDIIMPQLDGLGVLERLHKKELKKFPKVIVLSAVGHDKVTQRAINMG
                     VDYYIVKPFNFESFADRLLQISELETSKVQNKNKEIVYNEKINSELSLEVKITEILHE
                     VGVPAHIKGYQYLRTSIIDVVKNVELLGAITKELYPMIAAKYSTTPSRVERAIRHAIE
                     VAWTRGKIETINNIFGYTIHNNKGKPTNSEFVAMIADKLRLEQRVS"
     gene            9790..10314
                     /locus_tag="PLB33_03480"
     CDS             9790..10314
                     /locus_tag="PLB33_03480"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG62614.1"
                     /translation="MVNKEITVKSEKIFEGKIVNLRVDTVELQNQKYAKREIVEHKGA
                     ACILAVNNDKVIFVRQYRKAIEENILELPAGILNIAEEPPEGALRELKEETGYKAGKI
                     ELIYEFYTSPGFSNEKVYLFKASELVYEGTDLDEDECLETIEVDRDEAKKMLETGKIN
                     DSKTIIGLLHWLNS"
     gene            10445..11026
                     /locus_tag="PLB33_03485"
     CDS             10445..11026
                     /locus_tag="PLB33_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62615.1"
                     /translation="MNNTLKKLVKSENKKFIALILIFIGALILSALIYSLTGKGSLSE
                     INYESISKMNFLQIFGSSIKRNIIYFLAVIFLTYFGQGYLTMILFGFISVYYGLSVIY
                     IIRTVGMDLKYFMLTFTDYFIFFPILLYFTFISSSIAKYTKKAKNIETISRKFDIIIS
                     GYLRISLFYLLIVTAYSFVYSLYVLILSRLMVR"
     gene            11026..11892
                     /locus_tag="PLB33_03490"
     CDS             11026..11892
                     /locus_tag="PLB33_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOG62616.1"
                     /translation="MEITMYKEYLYQRNLSENTIDSYIYDVESFRNYLCEDYELELTE
                     TKKAHILTYLVTLQKNGKSSSTLSRTISALKNFFDFLKNEKLIKDNPAVSIHSPKQIK
                     KKPSILTEEEVAQLMLLPDKNTFKGIRDLAMLELMYSSGIKVSELISIKLSQINLSAG
                     IISIEGEKERIIPLSKYAISAIENYLNNFRDKNTDENDFLFINITGEPLTRQGIWKSL
                     KSYKKKMNLNKELSPQILRNSFAVHMLSHGADLGTIQELMGLNSLGAAQNYINNLEFK
                     SLEVFNKTFPRG"
     gene            12006..13184
                     /locus_tag="PLB33_03495"
     CDS             12006..13184
                     /locus_tag="PLB33_03495"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOG62617.1"
                     /translation="MKRTVLIVLDSVGAGELPDAAAYGDEGSNTLKNIYKNAKNFSLP
                     HLENLGILNIDGFEDLKSPEYFLGSVAKCSQKSKGKDTTTGHWEISGLILDKPFPTYP
                     DGFPEELISKFEKKIGRKVIGNYAASGTEIIKELGKEHVETGNIIVYTSADSVFQIAA
                     HEEVVPLDELYEICIIARKMLQGNNGVGRVIARPFIGEEGNYTRTGNRKDFSLEPYKD
                     TMLDFVKNSGREVYAIGKIEDIFANKGITYSNHTKNNEEGIEALIQAVKLDFEGLIFA
                     NLVDFDMIYGHRNNVQGYGDALMYFDGRLPEIMDNLKDDDILIITADHGCDPTTASTD
                     HSREYIPLIFYGKNIKENNNLGILETFASIGKTVLDIFDIKNDLKGTSVKNQIIKTGK
                     "
     gene            13314..14468
                     /locus_tag="PLB33_03500"
     CDS             13314..14468
                     /locus_tag="PLB33_03500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOG62618.1"
                     /translation="MKKILTIFFIFIILNMMVVTAYGIEPEDLQSRSAILINADDGRI
                     LFEKNANDKMQPASITKIMLMVLISERMADGQITLDQEMTISENAAGMGGSQIYLEAY
                     EVQTVENMLKAISMRSANDASVAMAEFMYGSEESCVKAMNEKAKELGMKDTVFTNVTG
                     LPDPEHLTTANDIAIMTRELLKYNYMDEYMLTWMDSVFVGKEKDSEQVLVNTNRLINN
                     YNGLLAGKTGYTTEAKYCLSAAARRNDTTLIAVVLGCDNTKIRFDEVTKLLNEGFANY
                     KNIVFHKAGDTITTSPVYCGKEETINIVSKENINYFTESNCKPEDFNLEYVIDENLKA
                     PLNVDKKIGRAILSKDGRVLGEFELYPGANVEKENLFKFFINNFLKTTIR"
     gene            14481..15209
                     /locus_tag="PLB33_03505"
     CDS             14481..15209
                     /locus_tag="PLB33_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOG62619.1"
                     /translation="MDYSVNINVFQGPFDLLFHLIEKKEIDIYDIPISEITDQYLEYL
                     EEMMQFNMNVASEFIFMAATLVEIKSQMLLPQKEKEEDPRQELVNKLLEYKMYKDLSE
                     ELKKYEDESSFYISKPREEMAITSDTKTEQLSLNEINVYELYNIYLSLIKKQNFRIEP
                     EEKFKIYREKYSVKGCMEDIVKKIKKFGKVSLFNTLKEKENISKEYIVTLFLAILELS
                     NNKGMKIYQENIYGDIIITKQERK"
     gene            15206..15730
                     /gene="scpB"
                     /locus_tag="PLB33_03510"
     CDS             15206..15730
                     /gene="scpB"
                     /locus_tag="PLB33_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOG62620.1"
                     /translation="MNIKSVLESLLFAWGEPLSIIEMSKILEKPADSIILVLDEMISE
                     FNDNEDRGLIIQKFGDSYQLTTKKANYEYIQNLLETTVSKSLSTAALETLSIIAYKQP
                     VTKVDIERIRGVKCSQVVKGLIDKGLIKEVGRLDKPGRPVIFATTDEFLRHFGLSSID
                     ELPSIGDIASTVEV"
     gene            15807..16178
                     /locus_tag="PLB33_03515"
     CDS             15807..16178
                     /locus_tag="PLB33_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62621.1"
                     /translation="MGILIVLLGGLLFLYLNIKCAFKINITFYFLHVYFYVIILRKKH
                     EYQKRINFSAKKIIDRYKAPELRQKTKNNLKYLKHIKKVFNIFYVKDIFFYPECILEK
                     QSFALEFVIVNRMLKKSLLNG"
     gene            16324..16704
                     /locus_tag="PLB33_03520"
     CDS             16324..16704
                     /locus_tag="PLB33_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62622.1"
                     /translation="MSKDIESIIKTTLDNINNLTENEKLISSKICYDNTNYLAISKLN
                     MNFIIGGSDIDKKFRTVDLKPFAGAAYVNLQLNPQILIYETPDNNIKTININNETTAG
                     DITSSIMNIMSFIKEIREKKDCDI"
     gene            16694..17725
                     /locus_tag="PLB33_03525"
     CDS             16694..17725
                     /locus_tag="PLB33_03525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOG62623.1"
                     /translation="MIFKRAIAILTVIFIFTCEFTCVEAMPGVSAQAYIVIDAQSGRI
                     LKSHNENKRLPIASTTKIMTAILAIETIDDLNQRIEVPPECSGIEGSSIYLRPKQMVS
                     VIDLLYGTMLRSGNDAAETLSKFAGRNNPEGFISMMNKKAKELGALNTNFENANGLHD
                     DNHYSTAYDLALISAYAMKNDTFRKIVSTEKYKAESMNNMFYNKNKVVYQYKYGTGIK
                     IGFTKAAGRCIVASAEKDGTEIIAVLLNDNNWFNDSYQIFDWAFENYKCYSIVEKGQF
                     VKYTPERNPVFIGESFRCVLTDKEIKQIRFEALIKPELMSNDSETKTCGTYNIYLGKT
                     KLGTGYLIR"
     gene            17877..18584
                     /locus_tag="PLB33_03530"
     CDS             17877..18584
                     /locus_tag="PLB33_03530"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOG62624.1"
                     /translation="MEERLQKYIARCGVASRRKAEEIILQGNVKVNGIIIKELGSKIN
                     PEKDIVTVNNQRIYEKQKNIYIKMYKPEGYITTVKDQFNRKTVLDLINIKERIYPVGR
                     LDYDTSGLLLLTDDGDLANKLMHPKYRIFKTYEAEIEGGISEDSINKLRNGVLIDGYK
                     TAPAKVKLLKKTQKNSFVQISIHEGKNRQVRKMFEKVGHRVIKLKRLSFGNINLDGLR
                     EGQWKYLTDDEIEYLKR"
     gene            18587..19159
                     /locus_tag="PLB33_03535"
     CDS             18587..19159
                     /locus_tag="PLB33_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG62625.1"
                     /translation="MDKMYGQITEMVHFLIKGSYEGKEDLRFVDLTCGNGYDTLFLSN
                     LAGKKGFVTAFDIQETAIERTKKLLQEESNFNNYKVIHNGHEHIDKYLTEKIDAAIYN
                     LGYLPNFNKDIFTKPETTICSINSLFPYLKNSGRIYITAYTSHDKGCEINSIYDYLIK
                     LDKKEYNVIHIKVINKNNTPPELFIIEKNS"
     gene            19389..19586
                     /locus_tag="PLB33_03540"
     CDS             19389..19586
                     /locus_tag="PLB33_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG62626.1"
                     /translation="MAEEKILLQNIPWCKGCGVCVGFCPVKVLEIKDDKCVIAHRDKC
                     IFCGQCELRCPDNAIWIGGKK"
     gene            19583..20719
                     /locus_tag="PLB33_03545"
     CDS             19583..20719
                     /locus_tag="PLB33_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOG62627.1"
                     /translation="MSNKVYKLVQGNEACVEGALAAGMRFFAGYPITPSTEIAEISAE
                     KLPALGGRFIQMEDELASMAAIIGGSLAGKKSMTATSGPGFSLMQENIGYAAITEIPC
                     VVVNVQRGGPSTGLPTSPAQGDVMQAKWGTHGDHPAIALTPNSVQETYEYTVKAFNLA
                     EKYRTPVILLMDETVGHMREKMAFKDVSEIEIIDRKKPTCKTEDYVAYRPDEDMIPPM
                     ASFGEGYRYHVSGLTHDETGFPTNNNEISEKLVKRLVDKVNKNLDDFSYYEEYKLEDA
                     EIVIIAYGGVSRSAKSAVDELRKQNIKAGLFRPITIWPFLEKQVNKVSLNAKEIYVAE
                     MNLGQYFLEVERAACKNCTVKNIGKVNGELITPEEIVQAIGGNK"
     gene            20719..21534
                     /locus_tag="PLB33_03550"
     CDS             20719..21534
                     /locus_tag="PLB33_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOG62628.1"
                     /translation="MNCNEITKKYYRANKLPHIWCPGCGHGTLTNTIVRAIDKLGLNK
                     DEVVIVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGIKFANPNLHVIVIMGDGDSSA
                     IGGNHLIHAARRNIDITAVVFNNNIYGMTGGQFSPTTPSGDMATTTPHGNIDKPFDLC
                     SLAQGAGATYVARGTAYHAVLMQKLIENGIQNKGFSFIEGLSLCPTYYGRKNKKGSAF
                     KMHTFLKENCVDKKVVDKDPEKGKNKILIGEFYNNPQPEYTESYQAVIDKLQG"
     gene            21545..22081
                     /locus_tag="PLB33_03555"
     CDS             21545..22081
                     /locus_tag="PLB33_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG62629.1"
                     /translation="MKKLEIRLSGSGGQGVILAAIILANAGVEQGLNAIQTQSYGPEA
                     RGGASKAEVILSNGEINYPKISTANILLSLTQKAYDKYKSSLEKDGILIVDESVKLRG
                     DETFKVYSLPILRTALEKFETHMVANIISLGAIYELMGENIIDKDIMIRSITERVPAH
                     TVQKNTDAFEEGIKLARG"
     gene            22083..22937
                     /locus_tag="PLB33_03560"
     CDS             22083..22937
                     /locus_tag="PLB33_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOG62630.1"
                     /translation="MNDLIRIIKKNFHKFSRGQKLIAQFIIDHYDKAAFMTAAKIAET
                     VDVSESTVVRFASALGYSGYPEMQKALQVLIKNKLTTVQRISLNDDVNDKLKLHKRNL
                     KNEMNNLRYLYDHFDMEALDKATELILEADRVFVLGLRTSSTLSNYLGFYLDVILNNA
                     KVLNNSGVNSLYEEIIRIKEDDLLIIISYPRYSRTTLDAARFVKERNTKIVAITDTEE
                     SPAYELADVSLLSKSNIVSFVDSLVVPMAMINQLIINISLREKEEIVEYFNTLERLWD
                     HNSIYQNF"
BASE COUNT         8955 a         3149 c         4339 g         6628 t
ORIGIN      
        1 aattaagcta tgcagtcaat gttttcatat tgctggtgat aatatcctta atattaaaac
       61 ttgatttttc aaaagtttca ggggagcctg taagttatca agatagtgaa gtgaaaacag
      121 aaagctatgt atatgcatta gaaaaaagaa tggaggaaat acttagtaaa attgatgata
      181 ttaacagtgt tgatgtaatg atttacacaa aacaaactcc gatactggaa cctgtttatg
      241 atgaaaatat cagcaatgaa acaaatattg aatctatgag tgacggtact aaaagagagg
      301 tgaacagaga gacaaaacaa aataaagtaa tattaggcag ggataattca gtagttgagc
      361 ggtattatca atatcctgaa atatccggtg tgcttgtggt agtaaattac agcggaaata
      421 aagatattta cagtattcta atcaattctg taaaaacttt attggatatc aaattaaatg
      481 atattgaaat aatagttata aactaagggg gtcttatgat aatgagaaaa gaaacattag
      541 tatttttagc atctctggca ataatattta ttattgggta tataaatatg agtttgacgc
      601 cggataatgc atttgatgat cttgaaacag gccagatgga gccggcagat ttggaaggtg
      661 acattgcaga aataattgag ggcggagtta atgaagttga ttttgcagaa gtgctgccgg
      721 aggaaacaga ttatgaaatt ttgggggata taacagattt atcgtctgct gaggaagctt
      781 cctcggaagg tgtcctgggt gttttaaatg ccagctttgc taactttaaa ttaaacaagg
      841 aaaaaagcaa catggatgta ttggattatc ttgaagaaag tttgagctca tcattgatat
      901 ctgacgaaac taaggagcaa tttgagcagc ttttattgaa aaagaattca tttattgaag
      961 cagaacaggg aattgagctt atgcttcaat caaaagggta taacgaatca gttgccattg
     1021 tagatgaaag catggttaaa attgtaacaa atgacacgat tgaaaaggca gatgccacag
     1081 ttattttaga tgtagtcgtt tcagaaacta attatacacc ttctcaaata aaaatcgtga
     1141 aatttgataa tattgatttg taaatatttt caacttctgc tataattatt ttaggaggtg
     1201 ctacaatgga caatgaaaaa tttgaattcg gtcaggtaaa aatatccgat gatgttgtaa
     1261 taattattgc aggtatagca gcgagtcaag ttaaaggcgt caatactact cgtacaggtg
     1321 taacagacgg aatcaccaat ttattttcaa aaaataatta ttcaaaaggt ataaaagtcg
     1381 atgttaatga agatacagta gtagtggaca tttttatcaa tgttgaatat ggctataaaa
     1441 taaatgaggt agcaagagaa gttcaattag caataaagaa agaaattgaa actatgacag
     1501 acttaaaggt ggcagctgta aacgtccacg tacttaatat cgttcaggaa aaaatacagg
     1561 agaaagtaca agaaaaagaa aaggatgctg ccaacatagc aatagagtag aggatggggg
     1621 acacaccata tggattgtcc ccctaatggt gcataaggaa gagtatacct tctgcggcag
     1681 taaaactttt atagtatcag gagtaaacat gaaacgtaaa gaaactagag aagaagctgt
     1741 aaaaattgca tacagtatgg acgtaaacaa agattttaac aaaaataata ttacagaata
     1801 tttagagcat tttgagcttg aaaacatgga tattgattat ttaaataaaa caatatccga
     1861 tatgattgac aatatggaag aaatagacaa acatattacc gataattcca aggactggaa
     1921 gctctcccgt attgcaaagg tagaccttgc agtcttacgt atagctcttt ctgaaatatt
     1981 atataatgaa acaatacctg aaagtgtatc aattaacgaa gcagtggaaa taagcaaaaa
     2041 attttcaaaa gaagactccc ataaattcat taacggaatc ttaggtactg tagtaaggtg
     2101 cataaaaaaa tgagatatat acttggaatt gatacaagct cttatacaac atccgttgca
     2161 cttattaacg aagaaagcaa tgaaatactt gcagatgaaa gaaaggtact cgaagtaaaa
     2221 aaaggacaaa aaggcctaag acagcaagaa gctgtatttc agcatttaaa aaacttgccg
     2281 gagctatacg aaaaaattcc atatgacctt tcaaatatta agattgtatc agttagttca
     2341 aaacctcgaa atgctgaaaa ctcatatatg cccgtattta ttgcaggaag aaatttcgga
     2401 aaggttattt ctgatacatt aaattgcgag tttttggaat attctcatca ggaaaaccat
     2461 atagcagctt caataataaa caattataaa aatatcgatg aattccttgc agtgcatata
     2521 tcaggtggta ccacggaatt tttgtcttgc aataaagcca ttaaaggctt cgatattaaa
     2581 attgtaggcg ggacaaagga tattacattc ggacagctca tagacaggct tggaatcatg
     2641 atgggtttta tatttccttg cggaaaatat atggaggaat acattaaaga caaaactttt
     2701 gaaaaaatta caagaccaag actgataaaa ggttcatata taaacttatc aggaatggaa
     2761 aactattttg gcaatctgct aaataaatat tcaaaggaag taataattac ttcacttttt
     2821 gattatattg ccgaatatat agtacatata ataagcagtc tgtcacatct tcaacacaaa
     2881 accttaatta tttcgggagg agttgcatcc aacagctata taagaaatta tatcacaaaa
     2941 agtgttaaag gatatgaagt attatttcct gctgtaaata attcttccga caatgcatta
     3001 ggtcttgcat atctccccat tattgacagg tggcacaatg aaattaaaac caattaaagt
     3061 ttctgtttta aaccaatata taaaaaagta cttaatgagt aattcaatcc tgaataattt
     3121 gagggttgag ggtgaaatta caaacattag aataagtaag acaggctata cttatttttc
     3181 attatgcgat gaaatatcct gtatatcttg tgttgctttt tttgccgaca caatttcaaa
     3241 aaacggggac aaaataatag cggaaggaga gctttccgta tacgaagcaa agggtaccta
     3301 ccagcttatt gttagagata ttgaaagggc aggattaggt aaaattcttt tagatttgga
     3361 gctattaaaa gaaaaactaa aagagcaggg actctttgac cgcaaaagag agcttcccga
     3421 attccctcaa agaataggca taattacctc aaagactggg gcagccatca aggatatatt
     3481 aaaaacattt gaaaatgtaa aagccaatat tgatataact atatacaaca cattggtaca
     3541 aggagaaaaa tccaaaaaaa gtattattga aggattaaaa tattttaaca gtgtggaaac
     3601 tgacgtaatt ctcatttcaa gaggaggagg aagttttgag gatttaaatg tttttaacga
     3661 tatggacgta gcaatggaag tttataaatc caatattgtg gttgttaccg gaataggaca
     3721 cgaaacagac agaacactga ccgactatgt ggctgatgta tactgtcata ctccgactgc
     3781 tgcagccgaa agaattatta gaaactacaa agatattgaa gaaaagctcg ataatcttgt
     3841 atttaccttg aaatcagaaa caaacaatac catatcatta aaaaaggctg aactaatatc
     3901 taataaatat ttattaagaa gcagcctgcc cttagaggat atttataaaa caaaaaatca
     3961 gttagatact tataaaaatt taataaaaaa taaaactacc tcacaaatta atgagtatta
     4021 tttcaatctt aaaataatgt tagaaaaatt gaatactttt gattacaaga aacagcttga
     4081 aaagggattt gcgttggtat cggacaattc aggaaatatt ataaagcata aagaacaggt
     4141 agaattaaaa gatattcttc atataagatt tgctgacttt aaaatagaag cagaaacaag
     4201 agatataaaa gaggtgtaat atggagtacg ataattttga aagtgccttg gaagaattaa
     4261 aaaatattgt ccggaaattt gaaaataaag atgatatttc aattgacgag cttctggaaa
     4321 attatgaaaa gggaatgaag gcttattctt tttgcatgaa aaagttggat gacactcaaa
     4381 gaaaaatcaa aattatagat gaaaatattg aataagaaat ccttagacat aagagggaac
     4441 attcctttga agacctgtat tataggctga cttccataaa ggtgtatcct taaattttaa
     4501 taattattaa aaatattaat tgatataagt taattgatat gctataattt tattgaatat
     4561 ccaaatattt atttttttag aagaatattt atgtaccggg caaggcataa aaatgaaaaa
     4621 ttacagactt gacgtatttt tgcataataa agggtatgtt ggcagccgtg aaaaggcaaa
     4681 ggagcttatt ttaaacaatt ctgtatttgt ggatgataaa ttaataacaa agccatcata
     4741 tatggtaaat gatgatgtta aaataaaaat tacagaagat gtatatataa gtaggggtta
     4801 tttaaaaatt gaaaaagcaa ttcatgtatt taacattaat attgaaaata agacagcagt
     4861 agatatcggt gcttcaaccg gaggatttac ggattatctg ttaaaacatg gagcaaaaaa
     4921 agtatatgca atagatgtgg gctgcggtca gcttcatgat tcattaaaga atgatattcg
     4981 agttgttaat ttagaaaaca caaatttcag atatattgat acatctataa ttactgatat
     5041 tatagacatt ataacagttg atgtttcttt tatttcgctt caacatatta tacctaaaat
     5101 tgtcgaatta agtcataatg atacagatat tgtcgcactg gtaaaacctc aatttgaagc
     5161 aggtaaggta aacataggga aaaaaggtat agtaaaaaaa agagaagtac atttaggggt
     5221 acttaatagt attaataatt attgtaaaac aaataatttg aaattaatta atatggatta
     5281 ttctccaata agaggagggg caggaaacat agaatatctt gcccacttaa aaattacagg
     5341 aaatacaaat gaaattaatt tcaaagactt aataaataaa gcctttgaaa aactataaaa
     5401 aaactaagag gtgtcaaatg aaaaagtata ccagacaaac aaaaattctt gagttaattt
     5461 caaaaagtga aattgaaacc caggaggaat tagctgaagc cctgaaaaat atgggtattg
     5521 atgttacaca agctacaatt tccagagata taaaggagct tagacttgta aaggtaatgt
     5581 ctaaaagcgg taagtataag tatgctacca taagtcaaag ccaggaagga ataacggaca
     5641 gattgaataa gatatttgaa aattccgttg tttcaatcga caatgcaatg aatatgataa
     5701 ttattaaaac aattccggga gctgctcaga tatgtgcatc agccattgat tttatgggaa
     5761 ttgaagaaat tgtaggaaca ttggcaggag atgataacgt ctttgtagct gtgcgaaaaa
     5821 ttgaacaggt agataaggta ttgcaggaat ttaaaaagaa tataagataa tggtgatgat
     5881 atgctgctta gtttaaatat aagcaatttt gcagtattcg aaaatgtatc tgttgatttt
     5941 gactccggat tcaatgtttt tacaggagaa accgggtccg gaaaatcggt tttgataaat
     6001 gcaattgagc ttattttggg ggatagagct tcaaaagaac taataagaaa aggcaaaaat
     6061 tctgcattaa tagaaggaat ttttgatata ggcgataatg acagcctgta tgaagactta
     6121 aagagtttaa acattgaaat tgataaggat gactttttaa taatatcaag ggagctttta
     6181 caaaacggca aaagtataaa caaggttaac ggaagaactt atcctcttaa caccattaag
     6241 gcaataggca gtttattgat tgacatttac ggtcaatttg gtcatgaaag cttatttaaa
     6301 aaagaaaacc atattaaaat gcttgacagt cttatacaaa atgagttgtc acccttgctt
     6361 cagtcctata atgagctgtt tgtcaaatat aaccgagtta taaacgaaat aagcacatta
     6421 aaagaaaaac tattaaataa agataaaaaa attgagcagt tacaatatga aataaatgaa
     6481 attgatagtg cagagttaaa ggaatttgaa gaagaggagc ttcttaaaaa tatcaaaaag
     6541 ctttctaaca tacaggaaat taaaaaatct ttgtatgatg caatacaaat tcttaacagc
     6601 gataatataa acaatgttta cagcattata aataaaatta aaaactatga cgaaaaactg
     6661 gaagaatttc tttcaagggt ggatattcag ttagaggaat taaaactatt aagctatgat
     6721 ttaagggact attcagaaaa tttggacctc gacgaaaaaa aactggataa tattgaaaac
     6781 aggatgtctc ttattaatcg tttaaaaaga aaatatggtt tttcaataga aaaaattaat
     6841 gaatacagaa atgcatccca tgaagaatta agtctgcttc ttaaagctga ttcaaaatta
     6901 aattctttaa ttacagaaaa aaatgaaata tataatttat tgatagaaaa agcaggtatt
     6961 atatctaaga aaagaaaaac cgctgcagaa tatcttgaaa agcagatttt aatcgaatta
     7021 agcgagctta atatgaaaaa tattgagttt gaagttaaaa tcaataaaaa aaatatatct
     7081 gcagacggtg ttgacgatgt tgaattttta atttccacta atgtaggaag cgacttaaat
     7141 tccgttcaga aaatagtttc cggaggagaa gcgtcaagaa taatgttagc ttttaaaaaa
     7201 ataaaaagcg acattggtca aaccatggtg tttgacgaaa tagacatggg aataagcggc
     7261 aagactgcac agatggccgg cgttaaaatg aattatattg caaagaacaa tcaagttatc
     7321 tgtgtcacgc attcacccca aattgcttca atttcaaaaa atcatttttt gattgaaaaa
     7381 aaagttgttg acaataatac attcagtaca gttaaaaagt tagacaaaga aaacaaaatt
     7441 tatgaaattg ccagattgtt gagcggaatg aaaataactg aaaaatctat aaataatgct
     7501 aaggaattga tagaatttaa cagtaaaata agtgaataga acaagtttta aatggaaaag
     7561 ttaaggataa aactatttag gagagcacaa tgaggaattt tctaagaagg gttttattct
     7621 ttactttttt tattgcggta ttaatgactg cggttttttt tggttttaat ttaaaaacaa
     7681 atccatataa atttgcaaaa ggcgaccaag taatagatac ggaaggaaag tacattatac
     7741 ccggtggtca gactatcgga atagagctga aaaccgaggg agtattggtt gtcggactcg
     7801 ctgatataat caatattgac aatatatcaa catctccggc aaaaattgcc gggattgaaa
     7861 ttggagataa aatattggaa atagactcta tatatatgag aagcaccgat gaaatattaa
     7921 attataccga aactatgggg attaaagtct atacatttaa aattgaaaga gccggggaga
     7981 tttacaattt gactataact cctgttcaaa gatatgacag cgatgaaatt aaattcggat
     8041 tttgggcaag agatgatata gcaggaatag gaacagttac atttgtagat ccggaaacag
     8101 gattctatag tgcaataggt cacggtattt ctgattcgga aacaggtaag ttgatagata
     8161 ttgaatcagg cactatatca aaagcaaata ttactaatat taagttaggc aaaaagggag
     8221 aaccgggaga aattataggc tacattttaa aagataaaga aagtcttgga acggtaaccg
     8281 ataataccga ttttggaatt tacgggaaaa taaataagga cagtatggga tatttcagcg
     8341 gcgatttaat ggaaatcgga aaaaaggaag aaattaaact cggtcctgca tatataattt
     8401 catgtgtaaa taatgaaata aaaaaatact cagtagacat aactaaaata ttttatcaaa
     8461 ataaacctga tgagaaaagc tttgttatta aaattacaga taaggaactt ttggaaatga
     8521 ccaatggtat tattcaaggt atgagcggaa gccctgtaat acaaaacaat aaaatagtcg
     8581 gtgctgtaac acatgtcttt atgaatgatc ctaccaaagg atatggtata tatattgaat
     8641 ggataattga caaaatatac gactgataaa attcattagt aaaatataac aaaaatttaa
     8701 aaacaaatat tttaatttat cgacaaaatt ttagtataaa ttaggaaaag ctgtatataa
     8761 tagacatata tactatttat attacaaaat tagaagacaa aaaatttttt ttataaaaaa
     8821 aaagggtttt ttgattcttt tgcgtattat attagtatag aatattttga atgggggagt
     8881 acttatgcaa aaaaaagtaa aagttgttat tgctgacgac aataaagaat ttaggttaat
     8941 tctaaaagac ttcttgctca gcaaaaaagt cttcgatgta gtagacatgg cagaggatgg
     9001 aataaaaaca ttggaatctg tcgaaaaaca tgaacccgac attcttatat tggacataat
     9061 aatgcctcag cttgatgggt tgggggtact tgagagactt cataaaaaag aattaaaaaa
     9121 gtttcctaaa gtaatagttc tatctgccgt aggtcatgat aaggttaccc agagagctat
     9181 taatatgggt gttgactatt atattgttaa gcctttcaat tttgaatctt ttgctgacag
     9241 attacttcaa atatcagaat tagaaacttc taaggtgcaa aataagaata aggaaatcgt
     9301 ttataacgaa aaaattaact cggagctcag cttggaagtc aaaataaccg aaattttaca
     9361 tgaggtaggt gtacctgcac atattaaagg ttatcagtat ttacgcactt ctataataga
     9421 tgtagtaaag aatgttgaat tattgggagc aattacaaag gaactgtatc ccatgatagc
     9481 agctaaatat tcaaccacgc caagcagagt tgaaagagcc atacgacatg caatagaagt
     9541 tgcttggact cgggggaaaa ttgaaactat caacaatatt ttcggttata ctatacataa
     9601 taataagggt aaacctacca attcggaatt tgtagcaatg attgcagata aacttcgcct
     9661 ggagcaaaga gtatcatagc aatagaaaat ggtgcacctg taaattaact tgacaaaaat
     9721 ctgtattgtt attagaataa gtccaactat taatcttgct tcagatgcaa tgatttaata
     9781 taggaggcta tagtgaataa ggaaattact gtaaaaagcg aaaaaatatt tgaaggcaaa
     9841 atagttaatt taagagtaga tactgttgaa ttgcaaaacc aaaaatatgc aaaaagagaa
     9901 attgttgagc ataagggtgc agcctgtata ttagctgtta ataatgataa agtaattttt
     9961 gtgagacaat acagaaaggc cattgaggag aatatattgg agctgccggc agggattctt
    10021 aatattgcag aagagccacc ggaaggagct ctgcgtgaat taaaagaaga aaccggttat
    10081 aaggcaggta aaatagagct aatttatgaa ttttatacct caccgggttt ttccaatgaa
    10141 aaggtatatt tgtttaaggc ttcggagctc gtatatgaag gtactgattt agatgaagat
    10201 gaatgccttg aaacaattga agttgataga gacgaagcaa aaaaaatgct tgaaaccggt
    10261 aaaataaacg atagtaaaac aataatagga cttctccatt ggctaaattc ttaaaataaa
    10321 aacataaaaa gtattaaacc tcttgtacaa gcatatatta tacaaagctt aacaagaggt
    10381 tttattgtgc aaaaaggacg tcttatgtcc tgcttggcat acagacagta ggtgggaggt
    10441 ttatatgaac aatacactaa agaagctggt gaaatctgaa aacaaaaagt ttatagcctt
    10501 aatattaatt tttatcggag ctttgattct atcggcatta atttattcat taacgggaaa
    10561 ggggtctcta agtgagataa actatgaaag tataagtaaa atgaattttc tgcaaatttt
    10621 cgggagttca ataaaaagaa atattattta ttttttggcg gtcatctttt taacatattt
    10681 tggacaagga tatttaacaa tgattttgtt tggttttatt tccgtatatt atggtttgtc
    10741 cgttatttat ataatcagga ctgttggtat ggatttaaaa tattttatgc tcacctttac
    10801 tgattatttt atattctttc ctattctact ttattttact tttatttcaa gttcaatagc
    10861 aaaatataca aaaaaagcaa aaaatatcga gactatttcc cgaaaatttg atataattat
    10921 atccgggtat cttagaattt ctttatttta tttactgatt gtaacagctt attcgtttgt
    10981 atacagctta tatgtattaa tattaagcag attgatggtg agataatgga aatcacaatg
    11041 tataaagagt atttgtatca gagaaactta agtgaaaata caatcgactc ctatatttac
    11101 gatgtggaaa gctttagaaa ttatctttgc gaagattatg agcttgagct aactgaaaca
    11161 aaaaaagcgc atattttgac atatcttgta actctgcaaa aaaacggcaa gagttcatca
    11221 acattatcga gaactatttc cgcattaaaa aacttttttg atttcttgaa gaatgaaaaa
    11281 ctgattaagg acaaccctgc tgtcagcata cacagcccaa aacaaataaa aaagaaacct
    11341 tcaattttaa ctgaagaaga agtagctcag cttatgcttt tgcctgataa aaatactttc
    11401 aaaggaatca gagacttggc aatgttggaa ctgatgtatt catcaggtat aaaggtttcc
    11461 gagctcatca gcataaaatt atctcaaata aacctaagcg ccggaataat ttctattgaa
    11521 ggagaaaaag aaagaataat tccattaagc aagtatgcaa taagcgcaat tgaaaactat
    11581 ttaaataatt ttagggataa aaacactgat gaaaatgact ttttatttat caatattacc
    11641 ggtgagccct taacaaggca gggaatatgg aaatctctaa aatcttataa gaaaaaaatg
    11701 aacttaaata aggaattatc tcctcaaatt ctaagaaatt cattcgctgt acatatgttg
    11761 tctcacgggg ctgacctcgg aactatacag gagttaatgg gacttaatag tctgggtgcc
    11821 gcacaaaatt atataaataa tttagaattc aaatccttgg aagtttttaa taaaacattt
    11881 ccacgcggtt gatattttca tacataattg atgtttcaga ctgcactatt aatggtataa
    11941 taagacaaaa ggaagagaga tataaattat ctcttctttt aatataaata aaggagcaac
    12001 caatgattaa aagaacagta ttgatagtct tagacagcgt gggagcaggt gaacttccgg
    12061 atgctgccgc ctatggcgat gaaggcagca atacactaaa aaatatatac aagaatgcaa
    12121 agaatttttc attaccacat ctggagaatt taggtatttt aaatattgac ggatttgaag
    12181 acttgaaaag cccggaatac tttttaggct ctgtggcaaa atgcagccaa aaatcaaagg
    12241 ggaaggatac aacaacaggt cactgggaaa tcagcgggct gatactggat aaaccattcc
    12301 ctacctatcc ggacgggttt cctgaagagc ttatttcaaa atttgagaaa aaaatcggga
    12361 gaaaagttat aggcaactat gctgcttcag gaaccgaaat aatcaaagaa ttaggaaaag
    12421 agcatgtgga aaccggcaat ataatagtct acacatctgc agacagcgta tttcaaatag
    12481 cagcccatga agaagtggtt cccttggacg agctctatga aatttgcata attgccagaa
    12541 aaatgttgca aggaaataat ggagtaggaa gagtaattgc aagacctttt ataggggaag
    12601 aaggtaatta tacaagaacc ggcaatagga aagatttctc cttagagcct tacaaagata
    12661 caatgctgga cttcgtaaaa aacagcggca gagaagttta tgccattggg aaaatagaag
    12721 atatatttgc taacaaaggt attacatatt caaatcatac caaaaacaat gaagaaggaa
    12781 ttgaagcatt aattcaagct gtaaaactag atttcgaagg cttgatattt gcaaatcttg
    12841 tagatttcga catgatttac ggtcacagga acaatgtaca gggatatggg gatgccctta
    12901 tgtattttga cggcaggcta ccggaaataa tggataatct caaggatgac gatattttga
    12961 ttattacggc tgaccatggc tgcgacccta caactgccag tacagaccat tcaagagaat
    13021 atatacccct gattttctat ggaaagaata taaaagaaaa taacaatctt ggcatacttg
    13081 aaacttttgc atccataggc aagaccgtat tggatatttt tgatattaaa aatgacctta
    13141 aagggacaag tgttaaaaac caaataatca aaaccgggaa atagactaaa ttagacattt
    13201 tttttaaaca actaacttag atatttcatt atccgcagat aggaatttgc tcatccaatt
    13261 aaatgtatga ttcaacaaaa tatggttatt ctacataaaa aggaggtaat tttatgaaga
    13321 aaattttaac catatttttt atatttatta tcttaaatat gatggttgtt accgcatatg
    13381 gaattgaacc cgaggacttg caatcccgct ctgcaatttt gattaatgca gatgacggaa
    13441 ggattttgtt tgaaaaaaac gctaacgata aaatgcagcc ggcaagcatt actaaaataa
    13501 tgcttatggt tttgatttca gaaagaatgg ctgatggtca aataacatta gaccaggaga
    13561 tgacaatttc ggaaaatgct gcaggtatgg gaggaagtca aatatatctc gaagcatatg
    13621 aagttcaaac agtagagaat atgcttaaag ctatatccat gcgctcagcc aacgatgcat
    13681 cagttgcaat ggctgaattt atgtacggct cagaagaaag ctgtgttaag gctatgaatg
    13741 aaaaagccaa ggaattgggt atgaaggata ccgtatttac aaatgttacg ggccttccgg
    13801 accccgagca tcttactact gcaaatgata ttgctataat gacgcgtgag ctgttaaaat
    13861 ataattatat ggatgaatat atgcttacct ggatggattc cgtatttgta ggcaaggaaa
    13921 aggattcaga gcaagtgttg gtaaacacaa acagactgat aaataattat aacggcttat
    13981 tggcagggaa gacaggttat acaactgaag caaaatattg tttatcggca gctgcaagaa
    14041 gaaatgacac tacattaatt gccgtagtcc tcgggtgtga taacactaaa attcgtttcg
    14101 atgaagttac aaaactattg aatgaaggat ttgcaaacta caaaaacatt gttttccata
    14161 aagcaggtga cacaataacc acatcaccgg tttattgcgg aaaagaagaa acgattaata
    14221 tagtttcaaa ggaaaatatc aactatttta cagaaagtaa ttgcaagcct gaggatttta
    14281 atttagaata tgtaatagat gaaaatctaa aagctccttt aaacgtggat aaaaaaatcg
    14341 gaagagccat actatctaag gatggtcggg tattaggcga atttgaattg tatccgggag
    14401 caaatgttga aaaagaaaat ttatttaaat tttttatcaa taacttttta aaaactacca
    14461 taagataagg aaggctggaa atggattact ccgtaaatat aaacgtcttt cagggacctt
    14521 ttgatctctt gtttcatcta atcgaaaaaa aggaaattga tatttatgat atacccatat
    14581 ctgaaataac agatcaatac ttagagtacc tcgaagaaat gatgcaattc aatatgaatg
    14641 ttgcaagcga atttatattt atggcagcaa ccttagttga aataaaatct caaatgcttt
    14701 tacctcagaa ggaaaaagaa gaagatccta ggcaggaatt agttaataaa ttactcgaat
    14761 acaaaatgta taaagattta tcggaagaac tgaaaaaata tgaggacgag agcagttttt
    14821 atatttccaa accaagagag gagatggcta taacttcaga tacaaagaca gagcagcttt
    14881 cactgaatga aataaatgta tatgaacttt acaatatata tttatctttg attaaaaaac
    14941 agaattttag aattgaacct gaagaaaaat tcaaaatata cagggaaaaa tacagtgtaa
    15001 agggctgtat ggaggatatt gtaaaaaaaa ttaagaaatt tggaaaagtt tcattattca
    15061 ataccttaaa agaaaaagaa aatatatcaa aagaatatat tgtaacattg tttttagcca
    15121 tattggaact ttcaaacaat aaaggaatga aaatatatca ggagaatatt tacggagata
    15181 taataatcac caaacaggag agaaaatgaa tataaagagt gttttggaaa gtttgctgtt
    15241 cgcttggggg gagcccttaa gcataattga aatgtcaaaa atattagaaa agcctgctga
    15301 cagcatcata ttagtattgg atgaaatgat aagcgaattc aacgacaatg aagacagggg
    15361 tcttattatt caaaagtttg gggattctta tcagctcact accaaaaagg ctaattatga
    15421 atatattcag aacctattgg aaactacggt atctaagagt ttgtcaacag ctgcattgga
    15481 aacactgtcc ataattgcat acaagcagcc tgttacaaag gtggatatag aaagaataag
    15541 aggggttaag tgctcccagg tggtaaaagg gcttattgat aaaggattga taaaagaagt
    15601 gggacgtctt gataagcccg gcagacccgt aatttttgct acaacagatg aattcttaag
    15661 acatttcggc ttaagctcaa ttgatgaatt gccgagtatc ggggatatag cttcaactgt
    15721 agaagtatga caaggggacg ggggtactgt catcttttta ttcaaaacaa agaaacagat
    15781 ttgtctgtta aagggttatt tttattatgg gaatattgat agttctattg gggggactct
    15841 tatttttata tttgaatatc aaatgtgcat ttaaaataaa tattactttt tattttttgc
    15901 atgtttattt ttacgtaatt attcttagaa aaaaacatga atatcaaaaa agaattaatt
    15961 tttccgcaaa aaaaataatt gacagatata aagctccgga attaagacaa aagactaaaa
    16021 acaatctaaa atatcttaag catataaaaa aagtatttaa tattttttat gtaaaagata
    16081 tttttttcta tcccgaatgt attttggaaa aacaatcatt tgctcttgaa tttgtaatag
    16141 taaacagaat gcttaaaaaa tctcttctta acggatgatt atgttataat tctttttttt
    16201 tagggcataa ttaaaataac ggaagaaatt gccacgcgat ttcaacactg ttaaacgagg
    16261 cagagaatat actcaccatt gtgattttgc ctcgttattg atttccaaaa gaaaggggat
    16321 aacatgagta aggatataga aagtataatc aaaacgacat tagataatat taataattta
    16381 acagaaaacg aaaagctcat aagcagcaaa atttgttatg acaataccaa ttatttggcg
    16441 atatccaagc ttaatatgaa ctttattata ggaggaagcg atatagataa aaaatttcgt
    16501 acagttgatt taaagccctt cgccggagca gcttatgtta atctgcaatt aaacccgcaa
    16561 attttaattt atgaaacacc ggataataat atcaaaacta ttaatattaa caatgaaaca
    16621 actgcaggag atattacatc ctccatcatg aatattatgt catttattaa agaaataagg
    16681 gagaaaaagg attgtgatat ttaagagagc aattgctata ttgacagtta tatttatttt
    16741 tacatgcgaa tttacatgtg ttgaagctat gcccggtgtt tcggcacaag cttatattgt
    16801 tatagatgct caatcaggaa gaattcttaa atcccataat gaaaataaaa gactgcctat
    16861 tgcaagcaca accaaaataa tgacagcaat tctcgcaatt gaaaccatcg atgatttaaa
    16921 ccaaagaata gaagtgcctc cggaatgctc aggcatagaa ggttcaagca tctatttaag
    16981 acctaagcaa atggtgtcgg taattgattt gttatacgga acaatgcttc gctcgggaaa
    17041 cgatgcggca gaaacgttat ctaaatttgc cggaagaaac aatccggaag gattcatatc
    17101 aatgatgaat aaaaaagcaa aagagttagg cgctctaaat acaaattttg aaaatgcaaa
    17161 cggccttcat gacgataatc attattcaac agcatacgac ttggctctta taagtgccta
    17221 tgccatgaaa aatgatacat tcagaaaaat tgtgagtacc gaaaagtaca aggctgaaag
    17281 tatgaacaat atgttctaca ataaaaataa agttgtttac cagtataaat acggtacggg
    17341 aattaaaatc ggctttacaa aggcagcagg ccgatgtata gttgcatctg cagaaaagga
    17401 cggtactgaa attatagcag tgcttctcaa tgacaataat tggtttaatg actcttatca
    17461 aattttcgat tgggcttttg aaaattacaa atgctacagc atagtcgaaa aagggcagtt
    17521 tgtaaaatat actcccgaaa gaaaccccgt ctttatcgga gagagcttta gatgcgtatt
    17581 gacggataaa gaaataaaac agattcgttt tgaagcatta attaaaccgg agcttatgtc
    17641 aaatgattcc gaaactaaaa catgcggaac ctacaacata tatttgggaa aaaccaagct
    17701 tggcacagga tatctgatta gatgacaagg ggatgggggc tttgtcatgt ttttgttcac
    17761 gcccaccagg gttgcttcct gaaaacgcat attgaatatt ttacaaattc atacatttat
    17821 aatataatat ctagaaaaca gggaaaagag cccatccctt gttatccgga ggagatatgg
    17881 aagaaagact gcaaaaatat attgcacgat gcggagttgc ttcgagacgt aaagctgagg
    17941 aaataatttt acaaggtaat gtaaaagtta acggaataat tataaaagag cttggaagca
    18001 aaattaatcc tgaaaaggat attgtaaccg taaataatca aaggatatat gaaaaacaaa
    18061 aaaatattta tataaaaatg tacaaacctg aggggtatat tacaaccgtc aaggaccagt
    18121 ttaatcgaaa aactgttctt gacttaatca atattaaaga aaggatttat cccgtaggaa
    18181 gacttgacta tgacacttcc ggattactgt tattaactga tgatggagac cttgccaaca
    18241 aattaatgca tcccaaatat cgcatcttca aaacctatga agcggaaatt gaaggaggaa
    18301 taagcgaaga ttccataaat aaattaagaa acggcgtttt aattgacgga tacaagactg
    18361 ctcctgccaa ggtaaaattg ttaaagaaaa ctcaaaagaa ttcgttcgta caaataagca
    18421 ttcatgaggg taaaaacagg caggtaagaa aaatgtttga gaaggtaggc cacagggtga
    18481 taaaattgaa aagactatca ttcggcaata tcaatttaga cggactgaga gaaggacaat
    18541 ggaaatattt aaccgatgat gaaatagaat atttaaagag gtaaagatgg acaagatgta
    18601 cggtcaaatt acggaaatgg tgcactttct aatcaaggga agctatgaag gaaaagagga
    18661 tttaagattt gttgatttaa cgtgcggaaa cggatatgac actctatttt taagcaattt
    18721 agcagggaaa aaaggttttg taacagcctt tgacatacaa gaaacagcaa ttgagagaac
    18781 aaaaaaacta cttcaagaag aaagtaactt caataattat aaagttattc ataatggaca
    18841 tgagcatata gacaaatatt tgacggaaaa aatagatgcg gccatatata acttaggata
    18901 tcttcctaat tttaacaaag atatttttac aaaaccggaa actactatct gctctataaa
    18961 ttcacttttt ccttatttaa aaaactcagg aaggatatat attacagctt atacaagtca
    19021 cgacaaaggc tgtgaaatta attcaattta cgactactta attaagttag acaagaaaga
    19081 atacaatgta attcatataa aagttataaa taaaaataac actcctcctg aattatttat
    19141 tatagaaaaa aattcataat actaattaat gacaataccc ccgtcccctt gtcatatgat
    19201 actgcttgct catttcttgc aaaatattca tttatatgtg cgatttagtt aaaaagcaac
    19261 gttttccttc caattaaatg cagataatga aataaaactt gcattatgca atattatttg
    19321 atatatttct tgcaagaaaa cctcgaatct gttataatta agataacgaa attatatgga
    19381 ggaattatat ggctgaggaa aaaattcttt tacaaaatat accatggtgc aaaggatgtg
    19441 gtgtatgcgt tggtttttgt cccgtaaaag tactggaaat caaagacgat aaatgtgtca
    19501 tagctcacag ggataaatgt attttttgtg gacaatgtga actaagatgt ccggacaatg
    19561 ctatttggat tggaggtaaa aaatgagtaa taaggtatac aaattagttc aaggaaatga
    19621 agcttgcgtt gaaggtgcat tagctgccgg tatgagattt tttgccggtt atcccataac
    19681 gccatcaacc gaaatagcgg aaatatctgc agaaaagctg ccagcgctgg gaggcagatt
    19741 tattcagatg gaagatgagc ttgcaagtat ggctgccata ataggaggct ctctcgcagg
    19801 gaaaaaatct atgactgcaa ccagcggtcc cgggttttct ttaatgcagg aaaacatagg
    19861 gtatgcagct attaccgaaa taccttgtgt tgttgtaaat gtgcaaagag gtggaccgag
    19921 tacaggactt ccgacatctc ctgctcaagg tgatgttatg caggcaaagt ggggaacaca
    19981 cggagaccat cctgctatag ccttgacacc taattctgtt caagaaacat atgaatatac
    20041 tgttaaagct tttaatttag ctgaaaaata cagaactccg gtcatactcc taatggatga
    20101 aacagtagga catatgaggg aaaaaatggc ttttaaagat gtttcggaaa tcgaaataat
    20161 agacagaaaa aaaccaacat gcaagactga ggactatgtt gcttatagac ctgatgaaga
    20221 tatgatacca ccaatggcat cattcggaga aggatacaga taccatgtat cgggacttac
    20281 tcatgacgaa accggttttc ctacaaataa taatgaaata tccgaaaaat tagttaaacg
    20341 attagttgat aaagttaata aaaatttaga tgacttttca tattatgaag aatataaatt
    20401 agaagatgca gaaattgtta taatcgcata cggcggagta tcaaggtcag ctaagagtgc
    20461 tgtagacgag ctaaggaaac aaaatattaa agcaggttta ttcagaccaa tcaccatttg
    20521 gcctttctta gaaaaacaag taaataaggt ttcattaaac gcaaaagaaa tatatgttgc
    20581 agaaatgaac ttgggacaat attttcttga agttgaaagg gctgcatgca aaaattgtac
    20641 tgttaaaaat ataggtaaag taaatggtga actaatcact cctgaagaaa ttgtacaagc
    20701 aataggaggt aacaaataat gaactgtaac gaaataacta aaaaatatta cagagctaac
    20761 aagctccctc atatatggtg tccgggatgc ggtcatggca cattaacaaa tacaatagta
    20821 agagcaatag ataaattagg acttaataaa gatgaggtag taatagtatc aggtatcgga
    20881 tgttcatcaa gagctcccgg ttatttggat tttaatactc ttcacacaac tcatggaagg
    20941 gcattggctt ttgcaacggg tattaaattt gcaaatccaa atcttcacgt tattgtaata
    21001 atgggagacg gagacagttc tgcaattgga ggcaatcacc taatccatgc cgcaagaaga
    21061 aatatagata taacagcagt agtattcaac aataatattt acggaatgac aggcggacaa
    21121 ttttcaccta ctactccttc cggtgatatg gcaacaacta caccacacgg caatattgat
    21181 aaaccttttg acctttgcag cttggctcaa ggagcaggag ctacttatgt cgccagaggt
    21241 acagcttacc atgctgttct tatgcagaaa ttaatagaaa acggaataca gaacaaagga
    21301 ttttcgttta tagaaggttt aagcctgtgt ccaacatatt acggaagaaa aaacaaaaaa
    21361 ggcagtgctt ttaaaatgca tactttttta aaggaaaatt gtgttgataa aaaggtagtt
    21421 gataaagatc ctgaaaaagg caagaataaa atattaatcg gcgaatttta caacaatccg
    21481 cagcctgaat atacagaaag ttatcaagct gttattgata aacttcaagg atagggggag
    21541 aaaaatgaaa aaattagaga taagacttag cggttccgga ggtcagggag ttatattagc
    21601 agccattata ttagcgaatg ccggagtcga acaaggatta aatgctatac aaactcagtc
    21661 ctacggacct gaagcaaggg gcggtgccag taaggcagaa gttatattga gcaatggtga
    21721 aataaattat cctaaaatat caacggcaaa tatacttctt tctcttactc aaaaagcata
    21781 tgataaatac aaatcatctc tggaaaaaga tggtatatta attgttgatg aaagtgttaa
    21841 actaagggga gacgaaacat ttaaagtata tagtcttccc atattgagaa ctgcactaga
    21901 gaaatttgaa actcatatgg tagccaatat aatttcatta ggtgcaattt atgaattgat
    21961 gggagaaaac ataattgata aggatataat gatacgctcc ataactgaaa gagttccggc
    22021 tcatacagtt caaaaaaaca cggatgcatt tgaagaaggt attaaattag caagagggta
    22081 acatgaatga tttaataaga attatcaaaa aaaactttca taaattcagc agaggacaaa
    22141 agctaatagc tcaatttatt attgaccact atgacaaagc tgcatttatg actgctgcaa
    22201 aaatagcaga aacagtagat gtaagcgaat caaccgttgt aagatttgca tcggccttgg
    22261 gttattccgg atatcctgaa atgcaaaaag ctttacaggt tttaattaaa aataaactta
    22321 ccacggtcca gagaataagt cttaatgatg atgtaaatga caagttaaag cttcataaaa
    22381 gaaatttaaa gaatgagatg aacaacttgc gttatttgta tgaccatttc gatatggaag
    22441 cattagataa agcaaccgag ctaatcttag aggctgacag agtatttgta ctagggctta
    22501 gaacatcttc aactttgtca aattatctag gcttctattt ggatgtaatc ttgaataacg
    22561 ctaaagtact caacaacagc ggtgttaatt ccttatatga agaaattata agaattaaag
    22621 aagatgattt attgattatt attagttacc caagatattc cagaacaact cttgatgctg
    22681 caagatttgt caaggagcga aatacaaaaa tagttgcaat tacagatacc gaagaatcac
    22741 ctgcctatga attggcagat gtatcactct tatcaaaaag caacattgta tcatttgttg
    22801 actccttagt agtacctatg gcaatgataa accaattgat aataaatata agcttgaggg
    22861 aaaaagaaga aatagttgaa tactttaata cattagaaag actctgggac cataacagta
    22921 tatatcagaa cttctaatat atgtgaggta atgggataca cctctgtttt gggaaagtct
    22981 ctggatcaga ggaatgtcct taaattaaaa gagcgacgct ctaaagtaag aataatcatt
    23041 aaggatataa agaaagggga gacgcttata a
//
LOCUS       DAOSHP010000021        22402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1856_length_22402_cov_12.552826, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000021 DAOSHP010000000
VERSION     DAOSHP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 22402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22402
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_22402_cov_12.552826"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLB33_03565"
     CDS             complement(<1..545)
                     /locus_tag="PLB33_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62631.1"
                     /translation="MFTILNKSNQSVNILSFYNKVYGILNKYRTLVLMLIDASIALFS
                     CHIPLLIFGLQDLEASTFILKLFLYTVIYILTFKANGMYKNLWRYSGYKDLLQCLKAS
                     AIASVLFLLVAWITKIPVDYGEFLLVSIISCFGTITVRLVYREMRTIVERKIDNEIPR
                     TNVMLIGAGQATALLLEELES"
     gene            complement(865..2226)
                     /locus_tag="PLB33_03570"
     CDS             complement(865..2226)
                     /locus_tag="PLB33_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62632.1"
                     /translation="MRTNKKIVYVCHCIDTEGPLYESLEATFERLETSIGIKIKPSQE
                     NLIKIQNQEMDLGGKEKVASLIVDKNLINYNDTWDKLDNMLDEMMSKPYRRKYADSFG
                     NGLIYNWFILDHIGYKTNPRRRDIGYHNIYNHYTVKIDEHDSYQDEINWHFHPMSAYM
                     EAHMCATSFINSPHLYESLARAVIDRCWFPSAFRAGFNTERPDSHWFLEQWIPFDFSN
                     QAIELTELDIQQMDNSDGRFGDWRRARADWAPYNPSHDDYQAEGSCRRTIFRCLNIGT
                     RIRLLNEDEVDKAFIRADCGTPTILAFTNHDFRDMRIDIENAHNLIMKTSKKYPDVRW
                     CNSGAKSAARKVLNIGNGDGINLTTRFDHKKDSVKITVETDSDSFGPQPFLAIKTFDN
                     KYMTDNFDFQIPRRKWTYTFDTNSINFKSVNKIGIATNDINGTTCVEVYDSTGEILQR
                     RKL"
     gene            complement(2249..3766)
                     /locus_tag="PLB33_03575"
     CDS             complement(2249..3766)
                     /locus_tag="PLB33_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOG62633.1"
                     /translation="MSAKSIVLVNSFFYTFSALLVRAMSFFLLPIYTRFLTPEDYGIT
                     NLVNSFNSVAIFIISFSLYIAVVRFYTDYKDDREQIKRFYGTIIIFIFISGLAFCIIV
                     IIFRYILIRLIIKDISFYPVGFMALFILLFLSLNTVHQNILEGMQQGKKLTVINFIIF
                     GSQVCLNMLFIGIYKLGALGILLATLITNLGYSLFMLYDLKKNNLITLCIDIRILKES
                     LKYSIPIMPHNLSTPIANFISRLLINNSSSLSFVGLYSIASQFGSIIDTVQASVNQAF
                     APWFYDMMNSCNQEARKDVVNFSYSLILIYSFIYMVIGLFSQEAIIIMTNERYIIAWT
                     AVPILVIAYSVKSIYYFYVSVLFFYKEASRKLFIATISGSFADIIIAFVLVPKYGMYG
                     AAFSFLVAKVIVVTIVIIISKRYDDIGFSVIKMIIIIIPSLLFMIAGLYFSYTKYLTV
                     FSWINLLYKLVVLSAYLVYIFITNKKNISIIINSEFIKQIIGELKSKNFKELSLK"
     gene            complement(3837..4943)
                     /locus_tag="PLB33_03580"
     CDS             complement(3837..4943)
                     /locus_tag="PLB33_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62634.1"
                     /translation="MISIFVCSTQFQLILSAMVSQEIRSKVSHRQVLLVDVYRDSLKT
                     FVRDNKEEIGKIWDEVYYINDNNSSNKSGILNNKLNGVKILFKVKKEIEEIFRKVKTT
                     EIKEIYIATGTYYSYVTKQLSRLGHQNNCIINIIEDGTSTYIYSKAKDNDYIIKESKK
                     IRNHINAVIKHILMYFLRYNNFDYDMADKIYVLNKDIYIGDESIKNKLIDIEVRQAIS
                     INLSMYPEDNIKDINRTDIIYFSQNIFDYEDEMIFLENLLSECNYCYKPHPSADNIIT
                     NNKILDSVPWEILCNYIEEDLIFISIASTAVFTPLLMYNKKNKAILLYKVLDINISKK
                     LDEFFIKVQNIYKDRVYVPEDSIQLQNVLKNIKK"
     gene            complement(4981..6303)
                     /locus_tag="PLB33_03585"
     CDS             complement(4981..6303)
                     /locus_tag="PLB33_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOG62635.1"
                     /translation="MDENFGRSMYEMADRLFPICRSLTGEGVRQTLSILKEVCPSMNV
                     YEVPTGTIAFDWKVPKEWNIKEAWIKDSNGNIVVDFKTNNLHVVGYSAPVNQKVSLNE
                     LMKYIYTDTSQPDIIPYVTSYYEEKFGFCMSDNHKNSLKEDTYHMVIDSELKDGSMTY
                     GEILIPSTNIDETDGSNKEVFLSTYICHPSMANNELSGPCLAIYLAKWIRELKNRRYN
                     YRIIFIPETIGSITYLSKNLDTMKKNIVAGFNISCVGDSNTYSYVASRHGNTLADRTA
                     KNILKYYYPEFKRYSFLDRGSDERQYNAPGIDLPVCGICRSKYGEYPQYHTSADNMNF
                     ISPEGLAGSFDVYVKCIEALENNYYYKTNFLCEPHLGKRGLYPATSRKGSVEKVRTMM
                     DFMAYADGEKDLIEISDIIDVPLYEILPIVEELMNKEVVIRVQKFVLR"
     gene            complement(6296..7012)
                     /locus_tag="PLB33_03590"
     CDS             complement(6296..7012)
                     /locus_tag="PLB33_03590"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOG62636.1"
                     /translation="MIAIIPARGGSKGVPKKNIRELSGKPLIFYTINAAQKSRYISRI
                     IISTDSFEIAEVAKSFGCEIPFMRPPELATDTSKALDAYLYTVEKLNTEFNEQIRDFS
                     VLLPTSPLRNAEDIDKAIEIFYSKRAKTVISVVEAEHPPTWYKKINDKGILEDYFRGE
                     DNSLNRQEAEKTYLPNGAIYIFNYNSLKLNCNYYNDETYPYIMSRKSSVDIDTEVDFK
                     LAELIIRSSQLNYTHGDYNG"
     gene            complement(7057..8106)
                     /locus_tag="PLB33_03595"
     CDS             complement(7057..8106)
                     /locus_tag="PLB33_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOG62637.1"
                     /translation="MKIRDKEIKCYSKPYIIADIGANHNGDMELAKKMIDCLAECGAD
                     AAKFQSWNDKSLISREEYDKNQKYDDSPKKHFGSLKEMVEKYYLREEQHFMLKEYCVS
                     KGIEFCSTAFSEQEADLLDKLEVPFFKIASMDINNLRLLKYIAKKGKPVILSTGMATI
                     AEIETAVKSIESEGNKNIIILHCISIYPPNPKDINLNNIIMLRQLFPNYPIGFSDHTI
                     GVSVPMASVALGACVIEKHFTIDKNLPGWDHEISADSTEMKFIVQESKIVADSMGNYY
                     RTVSAAEEEKKKKFRRSIVLAKDIKKGEVIKNDDITYKRPGTHIRPDEDMYVIGRKLI
                     RDMHEDDLLDWCDME"
     gene            complement(8090..8968)
                     /locus_tag="PLB33_03600"
     CDS             complement(8090..8968)
                     /locus_tag="PLB33_03600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG62638.1"
                     /translation="MKTALGTVLYKQSFKYLNEFSRSVNNQDCPDFDMLFLNDDLDEA
                     EIDISINSIKKKVILSSGKPNSTIPQLRYQLIKYAKENNYDLLALVDFDDTFSENRVS
                     KVKTAFNNEYGFYYNDIYYFYKKNKFFSFLPDYTNEVNQILEQNYLGLSNTAINLSKI
                     DFDLLEKLNVTICKAFDWYMYSVFLAEGHKGKKVDGCRTYYRIYENNTAGECKNTINY
                     INNEINTKINHYSLLKQWDSKYEELYDYYKALQHEWKRGNVDLLSYIDIKNDYWWGLI
                     KSNKVKEVIGYNENQR"
     gene            complement(8981..9631)
                     /locus_tag="PLB33_03605"
     CDS             complement(8981..9631)
                     /locus_tag="PLB33_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOG62639.1"
                     /translation="MKCKKLLLIGGGGHCKSVLDSILDNNDYIDIGIIEKKEICYGDI
                     LGISIIGCDSELEYYYNNGYQFAFVTVGNIGNPKIRIKLYKLAYKIGFKIPNIIDSSA
                     CVSRFTNLGRGIFIGKQAVVNAGTSIGDCAIINTSSVIEHDCIIEEFVNVAPGAVVCG
                     NVTVGRNSYIGAKSVIRQQLKIGSNTIIGMGSTVTKNIDSNVLAYGNPCRVINSKY"
     gene            complement(9621..10667)
                     /locus_tag="PLB33_03610"
     CDS             complement(9621..10667)
                     /locus_tag="PLB33_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOG62640.1"
                     /translation="MEIKDILIDENSTMIEAMNALDKTAKKILFVVKEDRLVAAITDG
                     DIRRWILAKGSLDARVKSIANYKPLFLYEKDKALARKYMKQNLIDALPIVDENKKIIT
                     VKFLYRQDIKNKVELDCPVVIMAGGLGSRLYPYTKILPKPLIPIGEIPIAEHIINRFQ
                     QLGINNFYMIVNHKKNMIKTYFNEIAKNYKIIYIDENQPLGTGGGLSLLKGIVKETFI
                     LTNCDIIIEEDIGKIYNFHKEKNNLITMVCSLKNITIPYGVINISEDGRIEDMKEKPV
                     LKFFTNTGMYVVESDIVNELENNKTIDFPDIIEQYKKSGGKIGIYPISEYSWMDMGQI
                     DVLENMRRRLENEM"
     gene            complement(10681..11853)
                     /locus_tag="PLB33_03615"
     CDS             complement(10681..11853)
                     /locus_tag="PLB33_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOG62641.1"
                     /translation="MAQRFIPLSVPNLKGRELQYVQDAIETEWVSTAGKYVNKFEEKI
                     ISYAITKGAVSCQNGTAGLHTALIVSGVTKDDEVIVPALTFIASVNPVKYIGAEPVFM
                     DCDDSLNMDTDKLEDFCRRECIFADGSLINKQTKKHIKAIIIVHVFGNMADMESIIEI
                     AREYNLIVIEDAAEAIGTYYTQGKYEGRHAGTIGDAGVYSFNGNKIITTGGGGMIVSN
                     DEDLLKRAKHLTTQAKTDELYYTHDDVGYNYRMTNVQAALGLAQLEQLETFIKIKIEN
                     YILYKEKIHEIPGLSLLNFNEDTRSNHWLYSLICGDEYPLNRDEIIMYLSSKGVQARP
                     IWGLINEQIPYLKNQAYKIEKAKFYVDHVVNIPCSTNLTKDEILYVVENLSNAALK"
     gene            complement(11855..12862)
                     /locus_tag="PLB33_03620"
     CDS             complement(11855..12862)
                     /locus_tag="PLB33_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOG62642.1"
                     /translation="MMKVLVTGGAGYIGAVLVRKLLKEGYEVRVLDSLKYGGESIIDL
                     LNNPKFEFVKGDIRDREIAYASTKNVDAVVHLAAIVGDPACSQFPVEAKEVNYDASAQ
                     LYEESLKNNVGRFIFSSTCSNYGKMDNSEKFLNEESELKPLSLYAQTKVEFEEYLLNQ
                     DKINVCKPVILRFSTVYGISPRIRFDLTVNEFTKELAMGRELLVYGEQFWRPYCHVDD
                     LANSILFVLESDEAKVEFNVFNVGSSDENYQKKMIINEILKQVPDGMVKYVTVNEDPR
                     DYRVNFDKIRDVLGFKVTKRVPDGINEIVTLIENGFIINPDDDKYYNIIRVRKPAILE
                     V"
     gene            complement(13024..14172)
                     /locus_tag="PLB33_03625"
     CDS             complement(13024..14172)
                     /locus_tag="PLB33_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG62643.1"
                     /translation="MNDTIATCKSKHPISVRCLFAWLVLMPFSFVALGSLGSILKLLT
                     IVIIVYYFIKGGRLKYRKSAILFTWFLYVIYYAVSLIWSLNLSDAIVYVIGYIQFFVL
                     AFLFSSEIMDNDDIRHLEDAWIFISIFCIIIFITGGDKSIEYSDRSTLYMLGTFTDPN
                     EFGGYFCIPTGLMCSRLFSENKRKSCIYLILIIVSTYIVLRTGSRGALLALIVCIIAA
                     MFRAARMSFKNTLFIIVLAILSYFIILNFMLPQISESVLNRFTFESLKADGGSERKLI
                     WTKSIKYFYEESIFRQVLGLGIFGGTFYKATMHNQLLQNLIDGGFIGLLLYCVLMVLG
                     FINIYKKYKNFVPGYIGMMLLSMTLTMTSSYKPLWTLMILGLIDIRRE"
     gene            complement(14191..15291)
                     /locus_tag="PLB33_03630"
     CDS             complement(14191..15291)
                     /locus_tag="PLB33_03630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG62644.1"
                     /translation="MKKVLIVIPTVRTGGAEKLALDISRLLDKNKFEATLLCYYSDQP
                     DAYESLIRDENIKIVYLNKKLGLDFTMFSKVKEVVELISPDIIHAHLDTLLYLIPSFN
                     RNQVKLFTIHSTPCYEASGILQKIIRTFCFHYKNVIPVAISQCFVQDIASYYQLNEKR
                     IACIPNGIDFGNFNTSKRKKTEDNIYKFITVGSMRPEKNQKLLIKAFAYVRKKNPNTQ
                     LTIVGDGTLKPEIEKLIAAYHLQDAVNLTGIVSNVGDYLASADAFVLSSDFEGLPLSL
                     LEAMACGLPIVSTKCGGTVDIIDDGVNGYLVDINDIGQLSDAMYRVTLLSEELRLRMI
                     EKSFEILPMYDIKRCTGDYERLYTKNIVTMLK"
     gene            complement(15324..16418)
                     /locus_tag="PLB33_03635"
     CDS             complement(15324..16418)
                     /locus_tag="PLB33_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG62645.1"
                     /translation="MKLLLIAPCPPPYGGISNWSKIVIDGIRRYFADIDLDVIDISPR
                     RRETEGRNLFNRAMDGITSTIKALCIAKKTLKNSKPDSVHLTTSGSLALLRDYVLLIF
                     FRKHQINTVYHIHFGRTAEIIKYGSWERILLMHAMKHAGSVIILDRLSFNSLREQFPL
                     LQVKKINNPIKIMGEPRNSTSKYYMFLGWVLKTKGIEELVSAWNQAGINATLRIVGPY
                     QESYLEYLHQAGYDFKSIIFEGEKGHDEAMEILSECEAFILPSYSEGCPYVILEAMSF
                     KKPIIATNVGDIPNMLSNECGILVEPHSSTQICEAIKSLESDSDLKIRIGSNAYKRVL
                     EEYSIKEILDLYRNEWESKYESIPFLLNIS"
     gene            complement(16454..>16921)
                     /locus_tag="PLB33_03640"
     CDS             complement(16454..>16921)
                     /locus_tag="PLB33_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOG62646.1"
                     /translation="DLIESLNEVAEKYKMPVIISTHPRTRNMIFTRGIEFHPLIQTMK
                     PLGFNDYIKLQINSKAVLSDSGTISEESSILGFKALNIRQANERPEAMEETSVMMVGL
                     KRERILQGIEILETQEKDTLRKVGDYCMPNVSDKVLRIILSYTDYVNRVVWGK"
     assembly_gap    16922..17021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17022..17285)
                     /locus_tag="PLB33_03645"
     CDS             complement(<17022..17285)
                     /locus_tag="PLB33_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOG62647.1"
                     /translation="MKRLKVMTVVGTRPEIIRLSAVINKLEESKVIEHVLVHTGQNYD
                     YELYEVFFEDFKLKKPDYFLNAANGTAVETIGNILIKIDPILEE"
     gene            complement(17282..18397)
                     /locus_tag="PLB33_03650"
     CDS             complement(17282..18397)
                     /locus_tag="PLB33_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HOG62648.1"
                     /translation="MKILITGAKGFIGKNLITELKNRKYTDLFEYDIDTNPVLLDKYC
                     KEAEFVFHLAGVNRPKEQSEYMEGNFGFTSILLDLLRKHKNICPIMLSSSIQAELDNP
                     YGKSKKAVEELLIDYGKKNGVKVLIYRFPNVFGKWCRPNYNSAVATFCHNVAHDLPIT
                     VNDPSVVMKLVYIDDVVNELISAYEGKENRKIEFCEVPIVYTITLGEIAKLLYSFKKS
                     REGRSIPNMSDVFIKKLYSTYLSYLPEDKFSYELKMNVDNRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHSKNEKFLVVSGKGVIRFRKIYSDEVIEYFVSGDKMEAVDIPPG
                     YTHNIENLGDIDMVTIMWVDESFEPEKPDTCYLEVTM"
     gene            complement(18431..19474)
                     /locus_tag="PLB33_03655"
     CDS             complement(18431..19474)
                     /locus_tag="PLB33_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG62649.1"
                     /translation="MFKDKTMLITGGTGSFGNAVMGKFLKTDIKEIRIFSRDEKKQDD
                     MRRHYKDDKLKFYIGDVRDIQSVKDAMHGVDYIFHAAALKQVPSCEFFPLEAVKTNVL
                     GTDNVLTAAIEHFVKKVICLSTDKAAYPINAMGISKAMMEKVFIAKSKTVDADKTLIC
                     GTRYGNVMASRGSVIPLFIDQIKSGQPLTVTDPNMTRFLMSLEEAVELVVFAFQNAEA
                     GDIMVQKSPASTIADLAQAVKELFNADNDIKIIGTRHGEKRYETLLTKEEYIKAEDMG
                     SFYRVPADKRDLNYDKYFIEGSQKLSSNGEYNSDNTERLNIEQIKEKLLLLECIRKEL
                     EYFNYRYETAITM"
     gene            complement(19467..20687)
                     /locus_tag="PLB33_03660"
     CDS             complement(19467..20687)
                     /locus_tag="PLB33_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG62650.1"
                     /translation="MKVLYIATSFPEPNKGATIYTDLAEALYEAGHEITVAVSEQAKN
                     LSRTQMKKERGFDVLRIVTGNYYDVSLIEKGITTLRIPIIMKNEISKYIVNRKFDFIL
                     YEAPPVTNASLVAWAKKKFDCPSYLMLKDIFPHNAVDIGIMKKSSIIYRYFIKKEKDL
                     YKSADIIGCMSEANKQYIIKHNSWLNESKIELLPNTKRITDEIEPVKLKIRQQYSIPE
                     DACVFIFGGNIGKPQYVDLLSQAIIECKNDEGIYFLFVGRGTERYKLENTIKSENIIN
                     AKVIENLPRDQYEQITKESDVGLIILDPRFTIPNYPSRILSYMEYSKPVLAATDKTTD
                     IKDLILQSKCGEWVWSGDKDKFVEAIRRMSKSPLLKQMGRNGRDYVKKNFSLESSVDI
                     LEKRFNKETRQQNV"
     gene            complement(20706..21428)
                     /locus_tag="PLB33_03665"
     CDS             complement(20706..21428)
                     /locus_tag="PLB33_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62651.1"
                     /translation="MANTNYECKHLQWDTDYFGVSSARVNLFGIVDEKGQEDIVEFCK
                     GYDFVTISNLDNTKENNHWIGNRTNAFLTDINVKFIKVLEDKPDYQDEKTYTANCLYK
                     NAQIVDIAGKSFNYSRFFNDSKLPEKKAKNIYIHWTECAFNNKDKYFVISEREGNVAG
                     YILFSFIEDRSVIELIAVDEKYQGQRVGKSLINTMESFVIDKGIGKIKAGTQVNNISA
                     TRFYSKMGFEYVSCGSVYHLWR"
     gene            complement(21421..22047)
                     /locus_tag="PLB33_03670"
     CDS             complement(21421..22047)
                     /locus_tag="PLB33_03670"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOG62652.1"
                     /translation="MYRKYFKRIIDFVLAVIALPFWFIILIIIGAIIYIQDEGPIFYN
                     AQRLGQDGKIFKMYKFRSMKVNSLDFRNEDGSTFNSEDDPRLTKIGKFIRKTSLDETP
                     QLLNIIKGDMSIIGPRPDLPEHRDLYEGNEERKLEVRPGVSGLNQAYYRNTVPWKERI
                     QNDIYYIDNLTILLDIKIFLKTVSSVLKRESVFIEHKSKSKSGVKANG"
     gene            complement(22058..>22402)
                     /locus_tag="PLB33_03675"
     CDS             complement(22058..>22402)
                     /locus_tag="PLB33_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG62653.1"
                     /translation="QSLIHFGEDFTSAGHLYLARIPNISENERNEIIIKMAENGISTN
                     VHYKPLPMLTAYKNLGFDIKDYPNSFDMYKNEITLPLHTLLSDEDVKYICESLKKAMN
                     KVLQTRQVVADK"
BASE COUNT         6855 a         4289 c         2996 g         8162 t
ORIGIN      
        1 tttgattcga gctcttctaa caataaagca gtcgcttgtc cggcaccgat gagcattaca
       61 ttcgtcctgg gtatttcatt atctattttt ctttcaacaa tagtccgcat ctccctgtac
      121 actaatctta cggtaatagt gccaaaacat gatataatgg atacaagcaa aaattcaccg
      181 taatcaacag gtatttttgt tatccaagca accagcaaaa acaatacgct tgcaatggca
      241 gatgctttca ggcactggag taaatcctta taacctgaat atcgccacaa atttttatac
      301 atgccgtttg ctttaaatgt tagtatgtaa attacagtgt acaaaaataa cttaagaata
      361 aaagttgatg cttcaagatc ctgtagtcca aatataagta aaggtatatg gcatgagaat
      421 aaagcaatac ttgcatcaat taacattaac actaatgttc tgtacttgtt taagataccg
      481 tatactttat tgtaaaaact taatatatta acactctgat tagatttatt taatattgta
      541 aacatctctc caatgacccc cccattaaaa tacaaatatc attgaaaata gagttcaaaa
      601 ttaattaata cttgtactta ttgtaaacca tatgaaatga gaagggtgtc gaataaaaaa
      661 gaagggggga aaaataattt aaaaaaattt gaaactaaat ctttacgttt cggtccaatt
      721 atttgacttc atatagccta gcagttggtc ttggtagtac tttggagaat ataataatgg
      781 catatcattt gctttttatt tgaagattgt tttagaatca taatatacaa ctataaccct
      841 cctctgatat tatagtatca ttttttatag ttttctcctt tgaagaattt ctccggttga
      901 atcatacact tcaacgcatg tggtcccgtt tatatcattt gtcgcaatgc ctattttatt
      961 aacacttttg aagtttatgg aatttgtatc aaaagtatat gtccatttgc gccttggtat
     1021 ttgaaagtca aaattatctg tcatatattt attatcaaat gtctttattg ccaaaaatgg
     1081 ctgcggtcca aaggaatccg agtcagtttc aactgtgatt ttaactgaat ccttcttatg
     1141 atcaaacctt gtggttaaat taatgccatc accattacct atgtttagta cttttcttgc
     1201 tgctgatttt gctcctgagt tgcaccatct tacatccggg tacttctttg aagttttcat
     1261 tattaggtta tgagcatttt ctatatcaat tctcatatct ctgaaatcat ggttggtaaa
     1321 tgccaaaatt gtaggtgtac cacaatcagc tcttataaac gccttatcca cctcatcctc
     1381 atttaataag cgaatccttg ttccgatgtt aagacatcgg aaaattgttc gtctgcatga
     1441 gccttcagct tgataatcat catgtgacgg attgtaggga gcccaatctg cccttgctcg
     1501 acgccagtct ccaaaccggc cgtccgaatt gtccatttgc tgtatatcca gctctgttaa
     1561 ttcaatagcc tgatttgaaa aatcaaaagg tatccattgt tctaaaaacc aatggctgtc
     1621 cggtctttct gtgttgaagc cagctctgaa ggctgaagga aaccaacagc ggtctatgac
     1681 tgcacgagct agggactcat acaagtgagg agaattaata aatgaagtgg cacacatatg
     1741 ggcttccata tatgcagaca taggatggaa gtgccagtta atttcatcct gatatgaatc
     1801 atgctcgtct atttttactg tataatgatt gtaaatatta tggtagccta tatctcttct
     1861 tctgggatta gtcttgtaac ctatatggtc aagtatgaac caattgtata tcaatccatt
     1921 accaaatgaa tctgcatatt tcctcctgta tggcttactc atcatttcat ccagcatatt
     1981 atccagcttg tcccaagtgt cattgtaatt tatgagattc ttatccacaa ttaaacttgc
     2041 caccttttct ttcccgccta aatccatctc ctggttttgg attttaatta agttttcttg
     2101 gctgggtttt attttaatgc caatacttgt ttcaagcctc tcaaaagtag cttccaagga
     2161 ttcatacaga ggtccttcag tatcgataca atgacacaca tatacaattt ttttgttggt
     2221 tctcattatt cacctcatta atatattttt atttcaatga taattcttta aaatttttgc
     2281 tctttagttc tcctatgatt tgtttgataa attctgagtt aattataata ctgatatttt
     2341 ttttatttgt tataaaaata taaactaaat aagctgataa tactactagt ttataaagca
     2401 agtttatcca actaaacact gtcaaatatt ttgtataact aaaatagagt ccagctatca
     2461 taaacaataa actaggtata atgattatta tcatttttat aacactaaac ccaatatcat
     2521 catatctttt acttataata atcactatag ttacaacaat tacttttgca actaaaaaag
     2581 aaaaagctgc tccatacatt ccatatttag gaactaatac aaatgcaatt ataatgtcag
     2641 caaaactacc actgatagtt gctataaaga gttttcttga tgcttctttg taaaaaaata
     2701 atacgcttac ataaaagtag taaatagatt taactgaata ggctattact aaaatgggta
     2761 cagctgtcca agcgattatg tatctctcat tagtcattat aatgatagct tcttgagaaa
     2821 aaagaccaat taccatgtat ataaacgaat atatgagaat taaagaataa gaaaagttta
     2881 caacatcttt tctagcttct tgattacagc tattcatcat atcgtaaaac catggggcaa
     2941 aagcctgatt tacagaagcc tgaaccgtat caataatcga accaaattgt gatgcaatac
     3001 tataaagacc aacaaatgat aatgaactgc tattattaat aagtaaacgt gaaataaagt
     3061 ttgcaatagg agttgataaa ttgtgaggca taataggtat tgaatatttt aatgattctt
     3121 ttaatatcct tatatcaatg catagcgtaa ttaaattatt ttttttcaaa tcatataaca
     3181 taaataaaga ataacctaaa tttgttatta atgttgcgag taaaatacct agtgcaccta
     3241 gtttataaat acctataaat aacatgttta ggcatacttg acttccaaaa attataaaat
     3301 ttattactgt caacttttta ccttgttgca ttccttccaa aatattttga tgtactgtat
     3361 tcagtgaaag aaataacagg ataaataatg ccataaaacc cacaggataa aaggaaatat
     3421 ctttaataat aagtctaatt aatatatatc taaaaattat tactataata caaaatgcta
     3481 atccggatat aaatatgaat ataattattg ttccatagaa acgttttatt tgttctctat
     3541 catccttata atcagtatag aatctgacta ccgcaatata caatgaaaac gaaatgataa
     3601 aaattgctac tgaattaaag ctattaacga gatttgttat cccataatct tctggcgtta
     3661 ggaatcgagt gtatattggc agtaaaaaaa aactcattgc cctcactaac aatgcactaa
     3721 atgtataaaa aaaggaattt actaaaacta tactttttgc actcattata atgtgatttc
     3781 tccttattca taattcaaaa gcgtaactct ctttaaaact cactgtattt aataattcat
     3841 ttttttatat tttttaaaac attttgtaat tgtattgaat cttccggcac atagactcta
     3901 tccttataga tgttttgcac cttaataaaa aattcatcta acttcttact tatattaata
     3961 tccaaaactt tataaagtaa aattgccttg ttttttttgt tatacatcaa aagaggtgta
     4021 aagacagcag ttgacgctat agatataaaa attaaatcct cttcgatgta attgcataat
     4081 atttcccatg gaacagaatc caatatttta ttgtttgtta tgatattatc tgcactgggg
     4141 tgtggcttat aacaataatt acattcactt aacagatttt ctaaaaatat catttcgtct
     4201 tcgtaatcaa atatattctg gctaaaatat ataatatctg ttctattaat atcttttatg
     4261 ttatcctcgg ggtacatgct taagttaatg cttatggctt gacggacttc tatatctatc
     4321 aacttgtttt taatgctttc gtctccgata tagatatctt tattgagtac atatatttta
     4381 tcagccatat cataatcaaa attattatac ctaagaaaat acatcaaaat atgcttaatt
     4441 acagcattaa tatgatttct tatttttttt gattccttaa ttatgtaatc attgtccttt
     4501 gcttttgaat atatgtatgt gctggttcca tcttcaataa tatttattat acaattattt
     4561 tgatgtccta accgtgataa ttgtttggta acatagctgt aatatgtacc cgttgcaata
     4621 tagatttctt ttatttcagt tgtttttact tttctgaaaa tttcttcaat ttcttttttt
     4681 actttgaata atatttttac tccatttaat ttgttattta aaatcccact tttattactt
     4741 gaattgttat catttatata gtaaacttca tcccatatct tgcctatttc ttctttatta
     4801 tcccttacaa atgttttaag tgaatctctg tatacatcta ctaaaagaac ctgcctatga
     4861 ctcactttgc ttcttatttc ctgtgatacc atagctgaca aaataagctg aaattgcgtg
     4921 ctgcaaacaa atatgctaat cataatttta cttcctttca ttattcatct atgcatctat
     4981 ttatcttagt acaaattttt gcacacgaat aacaacctct ttgttcataa gttcttccac
     5041 aataggcaaa atttcatata atggaacgtc aataatatca gaaatttcta tcaaatcttt
     5101 ttccccatct gcatatgcca taaaatccat cattgttcgt actttttcaa ctgatccctt
     5161 tctgctggtt gcaggataaa gacctctttt gcctagatga ggctcacata aaaagttcgt
     5221 tttgtaataa taattgtttt ccaaggcctc aatgcactta acataaacat caaatgaccc
     5281 tgccaagccc tccggcgaaa tgaaattcat gttgtcggca gatgtgtggt actgcgggta
     5341 ttctccgtat tttgaacggc atatgccaca tacaggcaaa tcaatacccg gtgcattata
     5401 ctgcctttca tcggaacctc tgtccaaaaa ggagtatctc ttgaattcag gataataata
     5461 ttttaatata ttttttgcag ttctgtctgc caaagtattg ccgtgtcgag atgcaacata
     5521 tgaatatgta ttactgtcac caacacatga aatattaaat cctgcaacta tatttttttt
     5581 catggtgtct aaatttttgc ttaaatatgt aattgagcct attgtttcag gtataaatat
     5641 aatcctgtaa ttatatcgtc tgttttttaa ttcccgtatc cattttgcca ggtatattgc
     5701 caaacaaggg cctgataatt cattgtttgc cattgacgga tgacatatat aggtggacag
     5761 aaatacttct ttattgctgc catcagtttc atctatattt gttgatggaa tcaatatctc
     5821 gccgtaagtc atgcttccgt ctttcaattc gctatctata accatgtggt acgtgtcttc
     5881 ttttaaactg tttttatgat tatccgacat gcaaaagcca aatttttctt cataatatga
     5941 agtaacatag ggaattatat caggctgaga cgtgtcggta taaatatact tcattaattc
     6001 attcaaacta accttttgat ttaccggtgc tgagtaacct acaacatgca gattatttgt
     6061 tttaaaatca acaacaatat tgccatttga atcttttatc caagcctctt ttatattcca
     6121 ttcctttgga accttccagt cgaatgcaat tgttccagta ggtacctcat atacattcat
     6181 tgaaggacaa acttctttta atatactaag agtttgtctc acaccttcac ctgttaaact
     6241 tctgcatatg ggaaaaagcc tatctgccat ttcatacatt gatcttccaa aattctcatc
     6301 cattataatc tccatgtgtg tagttcaact gactgctcct tataattagt tcagcaagtt
     6361 taaaatctac ttctgtatca atatccactg agctttttct gctcattatg taagggtatg
     6421 tctcatcatt gtagtagtta caatttaatt ttaacgagtt ataattaaaa atatatattg
     6481 caccatttgg caggtaagtt ttttcagctt cctgcctgtt caagctgtta tcttctcccc
     6541 tgaaataatc ctccaatata cctttatcat taattttttt ataccaagtt ggaggatgtt
     6601 ctgcttcaac aacagaaatc actgtttttg ccctttttga ataaaaaatt tctatggctt
     6661 tatcaatatc ttctgcattt cttaagggag atgtgggcaa tagtactgag aaatccctta
     6721 tttgttcatt aaactcagta ttaagtttct caactgtata taaataagca tccaaggctt
     6781 ttgatgtatc tgttgccagc tcaggaggtc tcataaatgg tatttcacac ccgaaagatt
     6841 tggcaacctc cgctatttca aagctatcgg tagaaattat aattctgctg atatatctcg
     6901 atttttgagc tgcattaata gtataaaaaa tcaaaggttt tcctgacagc tctcttatat
     6961 tctttttcgg aacacctttt gatccgcctc ttgccggtat aatagctatc atattttttt
     7021 gctcctttaa ttgccaagat ataacctcac tacaaattac tccatatcgc accagtccaa
     7081 taaatcatcc tcatgcatat ctcttattaa ttttcttcca attacataca tatcctcatc
     7141 gggccttatg tgtgtaccag gtcttttata agttatatca tcatttttta taacttcgcc
     7201 ttttttaata tccttagcca aaactatact tcttctaaat tttttctttt tttcttcttc
     7261 cgctgccgaa acagttctat agtaattacc catagagtca gcaacaattt tactttcttg
     7321 cacaataaac ttcatttctg ttgaatcagc agaaatttcg tggtcccaac caggtaaatt
     7381 tttatctatt gtaaaatgct tttctattac acaagctccc aaagcaacag aagccattgg
     7441 aactgaaacc cctatagtat gatctgaaaa cccaatggga taattgggga ataattgcct
     7501 aagcataata atattattta aattaatatc ctttggattt ggcggatata tggaaatgca
     7561 gtgtaatata attatatttt tgtttccctc tgattcaatt gatttaactg ctgtttcaat
     7621 ttctgctata gtagccatgc ctgtagataa aattaccggc ttgccttttt ttgcaatata
     7681 ctttaataat cttaaattat taatatccat ggatgctatt ttgaagaagg gtacttccaa
     7741 cttgtccaat aaatccgctt cctgttctga aaatgctgta gagcaaaatt caattccttt
     7801 tgaaacgcaa tattctttaa gcataaaatg ttgttcttct cttaaataat atttttctac
     7861 catttctttt aatgatccga aatgtttctt tggagagtca tcatattttt gatttttatc
     7921 atactcttcc cttgatatta aggatttatc attccatgac tgaaactttg cggcatctgc
     7981 cccgcattct gcaagacaat caatcatttt ttttgcaagc tccatgtctc cgttgtgatt
     8041 agctcctata tctgcaataa tataaggttt tgagtaacat tttatttctt tatctctgat
     8101 tttcattata acctataacc tccttcacct tatttgattt tataagaccc caccaataat
     8161 catttttgat atcaatatag cttaataaat caacatttcc tcttttccat tcatgctgca
     8221 atgctttgta ataatcataa agttcttcat acttgctatc ccactgtttt aataatgaat
     8281 agtggtttat ttttgtgtta atttcattat tgatatagtt tattgtgttt ttacattcac
     8341 cggcagtgtt attttcataa attctgtaat acgttctgca accgtcaact tttttccctt
     8401 tatggccctc tgcaagaaaa actgaataca tataccagtc aaaggcttta catattgtaa
     8461 catttaattt ttccagcaaa tcaaaatcaa tcttacttaa gtttattgct gtattgctta
     8521 aacccaaata attttgctca agtatttgat tgacttcatt tgtatagtcg ggcaaaaaac
     8581 taaagaattt attcttttta taaaaataat aaatatcgtt gtaataaaag ccgtattcat
     8641 tattgaatgc tgttttgact tttgaaaccc tgttttctga gaatgtgtcg tcaaaatcta
     8701 ccaacgccag caaatcataa ttattttctt ttgcatactt gataagctgg tatcttaact
     8761 ggggtatggt actatttggt tttcctgatg ataatattac tttctttttt attgaattta
     8821 tggaaatatc tatttcggct tcatctaaat cgtcatttaa aaacaacata tcaaaatccg
     8881 ggcagtcttg attgtttact gacctagaaa actcatttaa atatttaaat gattgtttat
     8941 ataaaacggt tcccagtgct gttttcactt tcaaccttct ttagtatttg ctatttataa
     9001 ccctgcatgg atttccatag gctaagacat tactgtctat gttctttgtt acagtgctac
     9061 ccatgccaat tattgtatta gaaccgattt tcaactgctg tcttataaca ctttttgcac
     9121 ctatataaga atttctcccc actgttacat tgccgcatac aacagcacct ggtgctacat
     9181 tgacaaattc ttcaataata caatcatgtt caataaccga gcttgtattg attattgcac
     9241 aatctccgat tgaagtacct gcatttacaa cggcctgttt acctataaaa attcctctgc
     9301 caagatttgt aaacctgctg acacaggcgg aagaatcaat tatattggga attttaaaac
     9361 caattttata tgcaagttta taaagcttaa ttctaatttt tggatttcct atgttgccaa
     9421 cggttacaaa agcaaattga taaccgttgt tgtaataata ttcaagctca ctatcacatc
     9481 caattatgct aatccccaat atatcgccat aacaaatttc ttttttttca attattccta
     9541 tgtcaatata atcattattg tctaatattg aatccaatac agacttacaa tgtcctcctc
     9601 cacctattaa cagcaatttt ttacatttca ttttccaatc gccttctcat attttctagt
     9661 acatctattt gtcccatatc catccacgaa tactcactta taggatatat acctatttta
     9721 cctccgcttt ttttatattg ttcaattata tcagggaaat ctatagtttt attattctcc
     9781 agttcattaa caatgtccga ctcaaccaca tacataccgg tgtttgtaaa aaacttaagt
     9841 acaggctttt ccttcatatc ctctattcgg ccgtcctcac ttatgttgat aacgccatat
     9901 ggaatagtaa tatttttcaa agaacagacc atagtaatta aattattttt ttctttgtga
     9961 aaattataaa ttttcccaat atcttcctca ataattatat cgcagtttgt tagtataaaa
    10021 gtttccttta caataccctt taataaactc aatcctcctc ctgtccccaa gggttgattt
    10081 tcatctatat aaattatctt gtaattcttt gctatttcat taaagtaggt ttttatcatg
    10141 ttttttttgt gatttacaat catataaaag ttgtttatgc ctagttgctg gaacctgtta
    10201 ataatatgct ccgcaattgg aatttctcct attggtatta agggttttgg aagtatcttt
    10261 gtatatggat acagcctgga tccaagtccc cctgccatga taactacagg acaatccagt
    10321 tcaaccttgt tcttaatgtc ctgcctatat aaaaatttta ctgtaataat ctttttattt
    10381 tcatcaacaa tgggtaaagc atcaattaag ttttgtttca tatatttcct tgctaaagct
    10441 ttatcctttt catacaaaaa taagggttta taatttgcaa tacttttaac ccttgcatca
    10501 agactgccct tagctaatat ccaccgcctt atatcgccat cagtgatagc agccaccagc
    10561 ctgtcttctt taacaacaaa aagtattttt tttgcggttt tatcaagggc attcatagct
    10621 tcaatcattg ttgagttttc atctatgagg atatccttaa tttccatagt atcaccactt
    10681 tcatttcagt gctgcattgc tcagattctc tactacatat aggatctcgt cttttgtcag
    10741 atttgtgctg cacggtatat ttaccacatg gtctacataa aattttgctt tctcaatctt
    10801 atatgcctga ttttttaaat atggaatttg ttcatttatc aatccccata ttggtcttgc
    10861 ttgtacaccc tttgaagata aatacattat tatttcatcc ctgttcaacg gatattcatc
    10921 cccgcatatt aaggaataaa gccaatggtt gcttctggta tcctcattaa agttcaataa
    10981 agaaagaccg ggtatctcat gaatcttttc tttatataaa atataatttt caatttttat
    11041 tttaataaat gtttctaact gttctaattg tgccagtccc aatgctgcct gtacattcgt
    11101 catccgatag ttgtatccca catcgtcatg ggtatagtac agttcgtctg ttttagcttg
    11161 agtagttagg tgctttgctc tttttagcag gtcctcatca tttgatacta tcattccacc
    11221 accgcctgtc gtaatgattt tattgccgtt aaatgaatag acacctgcat ctcctatagt
    11281 gcctgcatgc ctgccttcat acttgccctg agtataatat gttcctattg cttccgcagc
    11341 atcttcaatt actattaaat tgtattctct tgctatctca attatacttt ccatgtcagc
    11401 catatttccg aatacatgga caattattat tgcttttata tgctttttag tctgtttatt
    11461 aataagactt ccgtctgcaa atatgcactc ccttctgcaa aaatcctcca gtttatcagt
    11521 atccatattc aatgaatcat cacaatccat gaaaacaggt tctgcaccaa tgtacttgac
    11581 cggattaaca gatgctatga atgtcaatgc aggtacaatt acttcatcat cttttgtaac
    11641 accagatacg ataagggctg tatgaagacc tgctgttccg ttctggcaag aaactgctcc
    11701 cttagtaata gcgtaagata ttattttctc ttcaaattta tttacatatt ttcctgctgt
    11761 ggaaacccat tcagtttcaa tagcatcctg aacatactgc aattctctgc cctttaaatt
    11821 tggaacagaa aggggaataa acctttgtgc catactaaac ctccaatata gctggttttc
    11881 ttactcttat aatgttgtaa tacttatcat catctgggtt aattatgaaa ccattctcta
    11941 tcaatgtaac tatttcattt atcccatcag gtactctttt ggttacttta aatcctagca
    12001 catctctgat tttatcaaaa ttaactcgat agtctcttgg gtcctcatta actgttacat
    12061 atttaaccat accatcgggt acttgtttta aaatttcatt tattatcatt ttcttttgat
    12121 agttttcatc tgagctgccc acattaaaaa cattgaattc aacctttgcc tcatcacttt
    12181 ctaatacaaa caaaatcgag ttggccaaat catccacatg acagtagggg cgccaaaact
    12241 gttctccata taccaaaagc tccctgccca tggcaagttc ttttgtaaat tcatttacag
    12301 ttaaatcaaa tctaatgcgt ggtgatatcc cgtaaactgt tgaaaacctc agtattacag
    12361 gcttgcaaac attaatttta tcttggttga gcaagtattc ttcaaattca acctttgttt
    12421 gagcataaag agacaatggt tttagttcag attcttcatt caagaatttt tcagaattat
    12481 ccatctttcc gtaattagag caagtcgaag aaaaaatgaa cctccctaca ttgtttttca
    12541 aggattcttc atataattgc gccgatgcat cataatttac ttcctttgct tcaacaggaa
    12601 attgtgaaca agcagggtcg cctactattg ctgcaagatg cactactgca tccacattct
    12661 tggttgatgc atatgcaatt tccctgtctc tgatatcacc tttgacaaat tcaaacttag
    12721 ggttatttag caaatctata attgattctc ccccatactt aagcgaatca agaactctta
    12781 cctcataacc ttctttcagc aatttcctta ctaagacagc gcctatatag cctgcccctc
    12841 ccgtaactaa aactttcatc atagccatcc tccattttat taccttcaat tcttccttta
    12901 taaacttgtc caccttagat ttttccataa tgtaaatatt attttaggcg attatttttg
    12961 taagccttat aaaatttgag atgtgtcgaa ataaaaagca aggtaaaaat atttaagctt
    13021 aggttattct cttcttatgt caattaatcc taatatcatc aatgtccata gtggtttata
    13081 cgatgaagtc attgtcagcg tcatgcttaa cagcatcatt ccgatataac cgggtacaaa
    13141 attcttgtat tttttatata tatttataaa tcccaatacc ataagtacac aatataataa
    13201 caagcctata aatccgccat caatcagatt ttgtaataac tgattatgca tcgttgcttt
    13261 ataaaaagtt ccaccaaata tgcctaagcc caaaacctgc ctaaatattg attcttcata
    13321 aaaatactta atagattttg tccaaattaa ttttctttca cttccaccat cagcttttaa
    13381 tgattcaaat gtaaatcgat tgagaactga ttcagaaatt tgaggtagca taaaattgag
    13441 aataataaaa tatgacaaaa tggcaagcac tattataaaa agagtgtttt taaatgacat
    13501 tctggcagct cgaaacatgg ctgctataat gcatactatt aaagccagaa gtgcacccct
    13561 ggaaccggtc ctcaacacaa tatatgttga aacaataata agaatcaaat aaatacaact
    13621 tttacgcttg ttttcagaaa acaacctgct acacatcagt ccagtcggaa tacagaaata
    13681 tcccccgaat tcatttggat cggtaaatgt acctaacata tacaatgtac tcctatcact
    13741 atattcaatt gatttgtctc ccccggttat aaaaatgata atacaaaata tcgatataaa
    13801 tatccatgca tcttccaaat gcctaatatc gtcattatcc ataatctcgc tgctaaaaag
    13861 aaaagctaaa acaaaaaact gaatataacc aattacatat actattgcat ccgataaatt
    13921 caatgaccat attaagctga ctgcataata tataacatat aaaaaccatg taaataaaat
    13981 tgctgatttt cgatatttta atcgtccgcc ttttataaaa tagtatacaa taattacaat
    14041 cgtcagcaac ttcaatatag acccaagact gccaagtgca acaaatgaaa atggcatgag
    14101 tacaagccag gcaaaaaggc atcgcacgct aattggatgt tttgatttgc aagttgctat
    14161 tgtatcattc atggtaccct cgcaattcat ttatttaagc atagttacta tattttttgt
    14221 atataatctc tcataatcgc cagtgcagcg tttaatatca tacataggca aaatctcaaa
    14281 tgatttttca atcatacgta gtcttaactc ttcagaaagt agtgtcactc tatacatggc
    14341 atcactcagc tgcccaatat catttatatc cactaaatat ccattcactc catcatcaat
    14401 aatgtcaact gtaccaccgc attttgtaga aacaatcggg agaccgcatg ccattgcttc
    14461 caataaagac aatggaagac cttcaaaatc agatgaaaga acaaaagcat cggcactagc
    14521 taaataatcc cccacattac tgactattcc tgttaaattt acagcatctt gaaggtggta
    14581 tgcagctata agtttttcaa tttccggttt gagagttcca tcacccacaa ttgtcaattg
    14641 tgtatttgga ttttttttac gtacgtatgc aaatgctttt attagtaatt tctgattttt
    14701 ttcaggtctc attgatccta cagtaataaa tttatatatg ttatcttcag tttttttgcg
    14761 tttacttgta ttaaaattcc caaaatcaat gccatttggt atacatgcaa ttcgtttttc
    14821 atttaattga taatatgagg caatgtcttg aacaaaacat tgactaattg ccacaggaat
    14881 aacattttta taatgaaaac aaaaagtgcg gataatcttc tggagtattc cactcgcctc
    14941 atagcacggg gtactatgta ttgtaaaaag tttgacctgg tttctattaa atgaaggaat
    15001 caaatatagc aacgtgtcaa gatgtgcgtg aattatatca ggactaatca gttctactac
    15061 ttccttaacc tttgaaaaca tggtaaagtc aagtcctaat tttttattta ggtaaacgat
    15121 cttaatattt tcatccctta taagactttc gtaagcatcg ggctggtcac tataatagca
    15181 cagcaatgta gcttcaaatt tatttttatc tagtaaacgg cttatatcta aagctaactt
    15241 ttctgctcct ccagttctaa cagttggaat tactatcaaa acttttttca agataatctc
    15301 ctcatacagc aatttatata actttatgaa atattcagta aaaatggaat agattcatat
    15361 ttgctttccc attcgtttct gtataagtcc agaatttctt tgatagaata ctcttccaag
    15421 acccttttat atgcgttaga accaattcga attttcaaat ccgaatcaga ttctaaactt
    15481 tttattgcct cgcatatttg agtgctacta tgcggttcaa caaggattcc gcattcatta
    15541 ctaagcatat ttggaatatc accgacattg gtggctatta ttggtttttt aaatgacata
    15601 gcctctaaaa taacatatgg gcatccttca gaataacttg gcagaataaa agcttcacat
    15661 tctgacagaa tttccatagc ttcatcatgt cctttttctc cttcaaaaat aatgctttta
    15721 aaatcatagc cggcctgatg taaatactct aaataagact cttgataagg cccgacaatg
    15781 cgaagagttg cgtttattcc tgcctgattc catgctgaaa caagttcttc tatacccttt
    15841 gttttcaaaa cccatccaag aaacatataa tatttagatg ttgaattgcg gggttcgccc
    15901 ataattttaa ttggattatt tatcttcttt acttgaagca ggggaaactg ttctcttagt
    15961 gaattaaagc ttaatctatc aagaattatt actgaccctg catgtttcat agcatgcatt
    16021 agcaatatgc gctcccaact gccatattta ataatttctg cagttctgcc aaaatgaata
    16081 tgataaactg tatttatttg atgcttgcgg aaaaatatta acagcacata gtcccttaat
    16141 aacgccaggc tcccgcttgt tgtaagatga acagaatcag gtttgctgtt ttttaatgtt
    16201 tttttagcaa tacataaagc cttaatagta ctagttatac catccatagc tcggttaaaa
    16261 aggtttcgtc cttcagtttc ccttcttcgt ggagatatat ctattacatc aaggtcaatg
    16321 tcagcaaaat acctcctgat tccatcgatt acaattttac tccagtttga tattcctcca
    16381 tatggaggag gacatggtgc tattaataac aatttcataa atttcatacc tttctaaatc
    16441 cagttctttt ctgttatttc ccccaaacaa ccctattaac ataatcagta taagatagta
    16501 ttatcctaag taccttatct gaaacattgg gcatacagta atctccaact ttccttaaag
    16561 tatccttctc ttgagtctct aatatttcta tgccctgtag aattctttct ctttttagtc
    16621 ctaccatcat tactgatgtt tcttccatag cttccggtct ttcattggct tgtcttatgt
    16681 taagtgcttt aaatccaaga attgaagatt cttcactgat tgtaccgctg tcacttaaaa
    16741 cagctttgga attaatctga agcttaatat aatcattaaa cccaagaggt ttcattgtct
    16801 gtatcagtgg atgaaattcg atacctcttg tgaaaatcat gtttctagtt cttggatgag
    16861 tgctaataat cacaggcatt ttatattttt ctgccacttc atttagactt tcaattaaat
    16921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttcttcaag aatcggatca
    17041 attttaataa gaatattacc tatagtttct actgctgttc cgttagctgc attaagaaaa
    17101 taatcaggtt tttttaattt gaaatcttca aaaaacactt catatagttc atagtcgtag
    17161 ttttgtcctg tatgaacaag aacatgttct ataacttttg actcttctaa tttgttaata
    17221 acagcagata gtcttattat ctccggtctc gtaccaacaa ccgtcataac ttttaatctt
    17281 ttcacatcgt tacctccaag taacaagtat ctggtttttc aggttcaaaa gactcatcga
    17341 cccacataat ggtaaccata tctatatcac caaggttttc gatattatgt gtgtatcccg
    17401 gcggaatgtc tactgcttcc atcttatctc cacttacaaa atactctata acttcatctg
    17461 aatatatttt tctgaatcgg ataacgcctt taccgctgac tactaaaaat ttttcattct
    17521 tgctgtgatg ccaatggttt ccttttgtaa ttcccggttt agatatattt actgaaacct
    17581 gtcctctgtc cggtgttttt atgaattctg taaaagaacc cctgttatcc acattcatct
    17641 ttagttcata gctgaatttg tcttccggta aataactcaa atatgtacta tacagctttt
    17701 tgataaatac atcagacata ttcggaattg aacgcccttc tctgcttttt ttgaatgaat
    17761 acaataattt agcaatctct ccgagggtaa tggtatagac tataggaact tcgcagaatt
    17821 cgatctttct attttctttg ccttcataag cacttatcaa ttcatttacc acatcatcta
    17881 tatatacaag tttcattaca acacttgggt cgttcacggt tataggcagg tcatgagcaa
    17941 cattatggca gaatgttgcg acagcactgt tgtaatttgg tctgcaccat ttaccgaata
    18001 catttggaaa cctgtagatt aatactttta caccgttttt cttcccgtaa tcaatcaata
    18061 attcttctac tgcttttttg cttttgccgt aaggattatc taattcagct tgaatagaag
    18121 aagaaagcat tataggacag atatttttgt gctttctaag taaatcaagc agtattgatg
    18181 taaacccaaa atttccttcc atatactccg attgctcttt tggacgattc actcctgcca
    18241 aatgaaatac aaattctgct tctttacaat atttatcaag caaaacaggg tttgtatcta
    18301 tatcgtattc aaataaatct gtatattttc tgttttttag ttcagtaata aggttcttcc
    18361 ctataaaacc ctttgctcct gttataagta ttttcatcac ttatttcggc ctttctatta
    18421 atttagaaga ctacatggtg attgctgttt catatctata attaaaatac tccaattctt
    18481 ttcttataca ttcaagtaat agtaactttt ctttaatctg ttctatattc aacctttctg
    18541 tattatcaga attatactct ccgttagatg ataacttttg acttccttct atgaaatatt
    18601 tatcataatt caggtctctt ttatcagcag gcactctata aaagctgccc atatcttccg
    18661 cctttatata ttcttctttt gttaaaagag tttcatatct tttctctccg tgtcgggtgc
    18721 cgataatttt tatatcatta tcagcattaa acagttcttt aacagcttga gctaaatcag
    18781 caattgtaga tgcaggcgac ttctgaacca taatatcacc tgcttcagca ttctgaaatg
    18841 caaataccac caactctact gcttcttcca agctcattaa aaatctggtc atattggggt
    18901 ctgtaacagt taatggttgt ccgcttttta tctgatcaat aaacaggggt ataactgaac
    18961 ctctggaagc catcacatta ccgtaacgag ttccgcatat tagagtctta tcagcatcaa
    19021 ctgtcttaga cttggcaata aaaacttttt ccatcattgc ttttgaaatt cccattgcat
    19081 ttattggata agcagctttg tcagtagaga gacagatgac tttctttaca aaatgttcaa
    19141 tggcagctgt aagcacatta tctgtcccta aaacatttgt ttttactgct tctaacggga
    19201 aaaattcaca tgacggaacc tgcttaagtg ctgcggcatg aaatatataa tctactccgt
    19261 gcatagcatc ttttacagat tggatatccc ttacatctcc tatataaaac ttaagcttgt
    19321 catccttata gtgtcttcgc atatcgtcct gtttcttttc atcacgtgag aaaatgcgaa
    19381 tttcttttat atccgttttc aggaattttc ccattacggc attgccgaag gagcctgttc
    19441 cgcctgtgat tagcatggtt ttatctttaa acattttgct gcctcgtttc tttgttaaaa
    19501 cgcttttcaa gtatatctac agagctttct aaagaaaaat tctttttaac ataatccctg
    19561 ccgttccttc ccatctgttt aagtaacgga ctttttgaca tccttcttat agcttctaca
    19621 aacttatcct tatcaccgga ccatacccat tcaccacatt tagattgaag aatcaaatcc
    19681 tttatgtcag ttgttttatc tgttgctgct aataccggct ttgaatactc catgtaagat
    19741 agtattcttg atggataatt cggaattgtg aaccgaggat ctaaaattat gagtccaaca
    19801 tcactttcct tggtaatttg ttcatattga tctctcggta agttttcaat aacttttgca
    19861 tttattatat tttctgattt tattgtattt tccagtttgt atcgttccgt accccttccg
    19921 acaaataaga aatatatacc ttcatcattt ttacattcta taattgcttg gctcagcaag
    19981 tctacatatt gcggtttccc tatgttgccg ccaaaaataa acacacatgc atcctccgga
    20041 atggagtatt gttgtcttat ctttagtttt acaggttcga tctcatcagt aatcctctta
    20101 gtattaggaa gcaattcaat cttactctca ttcaaccaag aattatgctt gataatatac
    20161 tgcttgtttg cttctgacat acaaccaata atatctgctg acttatacag atctttctct
    20221 ttttttataa aatatctata aataatactg ctctttttca ttatccctat atcgacagca
    20281 ttatgtggaa atatgtcttt tagcattaaa tatgaaggac aatcaaactt ctttttagcc
    20341 catgcaacta agctagcatt agttacaggt ggagcttcgt aaagtataaa atcaaatttt
    20401 ctattgacta tgtactttga gatttcattt ttcattataa ttggaattct taatgtagtg
    20461 attccttttt ctataagact tacatcataa taatttccag taactattct taaaacatca
    20521 aatccacgtt cttttttcat ctgtgtccgg cttaggtttt tcgcttgttc tgaaactgct
    20581 accgttattt catgacctgc ttcatatagg gcttcagcca aatcagtata aatagtcgca
    20641 cctttattag gttccggaaa tgatgttgca atatataata ctttcattaa cctgtctcca
    20701 tactatcatc tccacaaatg atacacactt ccacaactca catactcgaa gcccatcttc
    20761 gaataaaacc gagtagctga aatattattc acttgtgttc ctgctttaat ttttccaatt
    20821 cctttatcta taacaaatga ttccattgta ttaatcagtg acttcccgac tctttgtcct
    20881 tgatacttct catcaacagc gatgagttca atcacacttc tgtcttcaat gaatgaaaac
    20941 agaatatagc ctgctacatt tccttctctt tcagagataa caaagtactt atctttatta
    21001 ttaaaggcgc attcagtcca gtgaatataa atattcttcg cttttttttc tggaagtttt
    21061 gaatcattaa aaaacctgga ataattaaat gactttcctg caatatcaac tatctgtgca
    21121 tttttatata agcaattcgc tgtataggtt ttctcatctt gatagtctgg cttatcctcc
    21181 aagactttaa taaacttaac atttatatca gtaagaaatg cattagttct attgcctatc
    21241 caatgattat tttcttttgt attgtcaaga ttagaaatag taacaaaatc ataaccttta
    21301 caaaactcta ctatatcttc ttgacctttt tcatctacaa taccgaatag atttactctg
    21361 gcagaactaa caccaaaata atccgtatcc cactgcagat gtttgcactc ataattggta
    21421 ttagccatta gctttcacac cactcttgct tttagatttg tgttctataa atacagactc
    21481 cctcttaaga accgatgaaa ctgtttttaa aaaaatcttg atatccaaca atattgtaag
    21541 attatcaata taataaatat cattctgaat tctttctttc caaggtactg tatttctata
    21601 ataagcttga tttaatcctg aaacccctgg tctaacttct agcttgcgtt cctcattacc
    21661 ttcatataaa tctctatgct ctggcaaatc gggtcttggc cctataatgc tcatgtcacc
    21721 tttaatgata tttaaaagct ggggtgtttc atcaagactt gtctttctga taaacttacc
    21781 tatcttagtt aatcttggat catcctcaga attaaaagta gacccatctt catttctgaa
    21841 atcaagagaa tttaccttca tagaacggaa tttatacatt ttgaatatct ttccatcttg
    21901 acctagtcgt tgtgcattat agaaaattgg accttcatct tggatgtata taattgctcc
    21961 gataataatc aatataataa accaaaaggg taaagctatt acagctagaa caaagtcaat
    22021 tattctttta aaatacttcc tatacatacc cttactccta cttatctgcc acaacctgtc
    22081 tggtttgcaa cactttattc attgctttct tcaaactttc gcaaatgtac ttcacatcct
    22141 catcacttaa caatgtatga agaggcagtg taatttcgtt cttatacata tcaaaggaat
    22201 tcggataatc ctttatatca aaacctaaat tcttatatgc tgtaagcata ggcagaggtt
    22261 tataatgaac atttgttgaa atcccattct cagccatctt tataataatc tcatttctct
    22321 cattttcact aatattgggt atcctagcta aataaagatg tcctgcagag gtgaaatcct
    22381 ctccaaaatg aattaaactt tg
//
LOCUS       DAOSHP010000022        22110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1892_length_22110_cov_8.881025, whole genome shotgun sequence.
ACCESSION   DAOSHP010000022 DAOSHP010000000
VERSION     DAOSHP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 22110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22110
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1892_length_22110_cov_8.881025"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PLB33_03680"
     CDS             <1..1064
                     /locus_tag="PLB33_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOG62654.1"
                     /translation="YKWDDVLLDAKLFQRIALIIPGLLIYMFASFYDNLQDIIQKVAI
                     IYILVIISLVFRSLLAAIDSIYKQNPVSKERPIKGLLQIIEIAIYVVVGIAVISFLID
                     KNPIYLLSGVGAVTAVVSLIFKDIIIGFVSGIQLVWNDMLRIGDWVEMPKYNTDGDVL
                     DITLYSVKIQNWDKTISTIPTSAFITDSFKNWKGMREYGGRRVKRSFLIDLNTIKICS
                     DEMVERFKKIDFISEYMEEITKEIDNYNKENIINTELHINGKQLTNLRVFRAYLTNYL
                     KNTIELRPSSTTIVRQLAPTENGIPIEIYLFVADTRWVNYETIQADVFDHILAVIDIF
                     ELRMFQNPTGNDIKELMKK"
     gene            1176..2180
                     /locus_tag="PLB33_03685"
     CDS             1176..2180
                     /locus_tag="PLB33_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG62655.1"
                     /translation="MVIISWLNRQLLKMEWLFELIRLLVENVFGLNIDDRLGGSIHFF
                     IYDVIKIFILLSVLIFTISYIQSYFPPERTKKILGRIKGVPANILGALLGTITPFCSC
                     SSIPIFIGFTSAGLPLGVTFSFLISSPLVDLASIILLASIFNWRTAIAYVVVGLLLAV
                     VSGTIISKLKFEEYVESYVFGKSSLDLEQEELTVNDRIEFSKNQVSDIIKRVWKYILI
                     GVGIGAAIHNWIPESLITAILGQDKWYSVIIAAVVGIPMYADIFGTLPIAEALIAKGV
                     GLGTVLTFMMGVTALSLPSMIMLKQVIKPRLLWTFIGLVSAGIIIIGYAFNAIGYMFI
                     "
     gene            2198..2437
                     /locus_tag="PLB33_03690"
     CDS             2198..2437
                     /locus_tag="PLB33_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG62656.1"
                     /translation="MIIKILGSGCKNCINLKENTEKALKETGIDAEIIKVENMKDIAS
                     YGVMRTPALVIDEKVVSYGKVLKPGDIAGLLEKYK"
     gene            2456..2722
                     /locus_tag="PLB33_03695"
     CDS             2456..2722
                     /locus_tag="PLB33_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG62657.1"
                     /translation="MKKNYEQAALLFRALSDPNRLAIIDMIKTEEKCACKILEELQIT
                     QPTLSHHMKILCDSGLVNSRREGKWMHYSINKELFEEVKQLFDF"
     gene            2757..3305
                     /locus_tag="PLB33_03700"
     CDS             2757..3305
                     /locus_tag="PLB33_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOG62658.1"
                     /translation="MHIKKNNSCCSCSDGCCGSSAKPEINKREIVIDFLYLDVTVCER
                     CQGTDKSLDEAIGEVANILAATGIDVKVNKINVLNEELAIKHKFLTSPTIRINGKDIQ
                     MDYKESLCESCGDLCGDEVDCRVWIYQGKEYSSPPKAMIIEAILKEVYGEKSEKQEKK
                     EYVIPENLRKFYKSRRSKQNYS"
     assembly_gap    3348..3447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3448..5000)
                     /locus_tag="PLB33_03705"
     CDS             complement(<3448..5000)
                     /locus_tag="PLB33_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOG62659.1"
                     /translation="MNNLDLIIKRRIFGIISHPDAGKTTLTEKLLLHGGAIRQAGTVR
                     ARKNARYAKSDWMEIEKQRGISVTSSVMQFEYDDKVISIMDTPGHNDFGEDTYRILTS
                     VDSAVMVIDAAKGIEAQTKKLFKVCSMRGIPIFTFINKLDREAKDPLELMQELEDVLG
                     LPSVAVTWPIGCGKEFEGIYDRQRNVVNLYKKKEIINLNEEDVKSNKLDGKIGPSNLT
                     NLRNAMELLDGAGNEFDIDLIRKGKLSPVFFGSALADFGVTEFLKHFLIMSPPPSSRK
                     TTTGEVHPTDDVFTGFIFKIQANMDPNHRDRLAFLRICSGTFVRGMNVTLTRTGKQMK
                     LSQSTHLMANERETVDTAYAGDVIGIYDSGNFQIGDTLTNGRDLIFFEPLPTFPPELF
                     SRVSPINSLKGKNFQKGVEQLAQEGAIQVYRNEYNEVILGAVGVLQFEVFKFRLVNEY
                     NTDIRMENIEFTVARWVKTDNPESLKRYQNSRCMLVFDHYERPVLLFTNQYALKSFQE
                     RYEDVELVEA"
     gene            5331..6653
                     /gene="miaB"
                     /locus_tag="PLB33_03710"
     CDS             5331..6653
                     /gene="miaB"
                     /locus_tag="PLB33_03710"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOG62660.1"
                     /translation="MKGRRKTYHILTYGCQMNEHDSEKISGMLTSIGYEETDDEKSAD
                     LVIFNTCLIRENAELKVFGKLGEVKGLKRNKPDMLVAVCGCMMQKQEIREKLLKKFSF
                     VDIIFGTNTIHELPLLIYNVEINKRKSMGIVDNTESIYENMPKQRKYKYKALVNITYG
                     CNNFCTYCVVPYVRGREKSREPNEIIDEIKTLAEDGCKEVTLLGQNVNSYGSNLDKRV
                     TFAELLYEINKIEGIERIRFMTSHPKDLTDDLIKAIKECDKVCNHVHLPVQSGSNDVL
                     NRMNRKYTREHYLNLVEKLKTAVPDIAITTDIIVGFPGETEEDFLDTIDIVKKVEYDS
                     AFTFLYSIREGTKAAKMENQVPDNVKHERFERLLEVLYPIILDKNKQCIGKVFPVLVE
                     SFENGKLTGRTEHFRLVHFNGSKDLIGKIVNVKITNVKTFHTEGEIVE"
     gene            6691..9312
                     /gene="mutS"
                     /locus_tag="PLB33_03715"
     CDS             6691..9312
                     /gene="mutS"
                     /locus_tag="PLB33_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG62661.1"
                     /translation="MAKYTPMMEQYLAIKEQYKDCILLYRLGDFYEMFFDDAITASRV
                     LEIALTGRDCGQEERAPMCGVPHHAVDGYIAKLIENGYKVAICEQLEDPSDAKGIVKR
                     DVVRIITPGTIIEQGMLDEKSNNYLCTIYLDKGFGMSYVDISTGELYVTENISLNDFL
                     KNSTHDLLIEEINKITPSEIISNNHIGNEVIDSMIYLSNVLSLDDYKQIVLKHFKVLS
                     LDSLGLKENYHAVISLGMLLNYLNETQKTSLDHINKLHFYTVGEYLQIDANTRKNLEL
                     IETIRGKKGPGTLYHVVDNTMTAMGGRLLKKWIEEPLKNIKHIYLRLDAVEELYENIM
                     TSNNIKEYLKSVYDIERLISRIVYGSCNGRDLNSLKQSLSNLPDLKSELNELSSQMFA
                     DIYSSFDELKDIFDLIDASIVEEPPISIKEGGIIKTGYNEELDELREITVNGKDWISN
                     LQAKEREATGIKNLKIGFNKVFGYYLEVTKSYLKDVPDNYIRKQTLANCERYVTPELK
                     EMEAKILNADEQIMKLEYELFLEIREFISRHVKRIQETAYNIAVIDAINSLAIAAVKN
                     NYVRPEMNKKGFIKITDGRHPVIERIMKNEMFVPNDTYIDNKEYRMSIITGPNMAGKS
                     TYMRQVALIALMSHIGSFVPAKKAEICIIDKIFTRVGASDDLSQGQSTFMVEMSEVSN
                     ILKNATSDSLLILDEIGRGTSTYDGLSIAWSVVEYITKKLKAKTLFATHYHELSELES
                     KLKSVKNYKILIKEEHDEITFLRKITEGSIDRSYGIQVANLANLPEEVIERAKEILIQ
                     LEESDINKPFARKKNRVNDNFQVSMFQANPAENEINKKYKELAENIKNIDIENITPMQ
                     SFKLLNDIIEKTKRIDT"
     gene            9330..11171
                     /gene="mutL"
                     /locus_tag="PLB33_03720"
     CDS             9330..11171
                     /gene="mutL"
                     /locus_tag="PLB33_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOG62662.1"
                     /translation="MAIINLLDNETINKIAAGEVIENPKSIVKELVENSIDAGADEIT
                     IEIKNGGKSFIRVTDNGKGIEKDQVENAFKRHCTSKISSSDDLNSLFTLGFRGEALAS
                     IAAVSKVEVITRTNDDIYGIKMTIEGGKIISKEDIGCPVGTTITVTDLFYNVPARKKF
                     LKSDISESSNINEIVVSLALSKENISFKYINNNNVAYRTPKTNNLLNTISSLYEKDLY
                     NSLLKIDYKSDIISITGYTTNLNYYRGNRKQQIVFVNGRVIKHRRMSYFIEEAYNTLL
                     PKNKYPACFIKIEIAPALVDVNVHPAKTEVRFQNEEMVLNEIKNAVYSALHSKSIMKE
                     VKGETLLHDSKDINNLRIFDRSSGKSVILGGLENKFTEETEETKNIRITEVEVSNKDI
                     AVMEPLKPYDKFEFKQQTFIKAENDEDKLPELYVIGIVLDTYIICENPEKTCIFMIDQ
                     HAAHERINYEKFLNQYNSREILIQEIVPEVINLSYDDYETAMSNVEIFEYLGISVENF
                     GNNAILINNIPTIFKDSNLKDVFYAILDNLKDITKSNLDFVMEKIIKEACVKSVKSGD
                     RLHMSEVRALLNDLRMTDNPYTCPHGRPVIIKMTRYEIEKMFERIQS"
     gene            11181..12131
                     /gene="miaA"
                     /locus_tag="PLB33_03725"
     CDS             11181..12131
                     /gene="miaA"
                     /locus_tag="PLB33_03725"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOG62663.1"
                     /translation="MNKIIVIVGPTAVGKTYISVELAKKLNTEIISADSMQIYKGMNI
                     GTAKITEEEKQGIIHHMIDIINPDEEYSVSEFKYDAEKIIDRLLSENRIPIIVGGSGL
                     YVNSLIYDLDFGNTKSNKKLREYYTYYYKEHGEDALYDKLLKIDPVAAEKIHKNNIKR
                     IIRALEVYDITGVKFSELNTDIRKKSNKYDCILIGLSMERKVLYERINQRVDEMLSKG
                     LVDEVSSLIKKGYGKNLVSMRGIGYKEIIEYLEGNTDYEEAVNTLKQNTRRFAKRQYT
                     WFLKDENVKWFSMDNPSEIDKTLDSIVEYISTRIDSISNQ"
     gene            12223..12459
                     /gene="hfq"
                     /locus_tag="PLB33_03730"
     CDS             12223..12459
                     /gene="hfq"
                     /locus_tag="PLB33_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOG62664.1"
                     /translation="MNIINLQDTFLNKVRKEKITITLYLNNGFKLTGTVLGFDNYVIF
                     IETDKGQQLVYKHAITSILPFKQVRLFFDEPEEQ"
     gene            12459..13757
                     /locus_tag="PLB33_03735"
     CDS             12459..13757
                     /locus_tag="PLB33_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOG62665.1"
                     /translation="MLTENKLCKQFGISEAVYNYVTKIEKEIKKNHFEKIDKIRELNQ
                     YKVIHAMQENKLDYTNFYWNTGYGYGDVGREKVEKIYASVFKTEDALVRPSIASGTHA
                     LYLTLSGILQHGDEVISISGRPYDTLLTVLGVQGKEPNNLSEAGIIYKEIPLYKNDID
                     WESAIKEVSMKTKLLMIQRSTGYSFRPALTIEKIKNAIERIRQVYPNIYIMVDNCYGE
                     FVDEMEPSDVGADVVVGSLIKNPGGGVALSGGYVVGKKVLIDRIANRLTAPGVGKEIG
                     LTFGTTRNTLQGLFFAPHIVSESLKGALLFGGVYSSLGFEITPTVEEDRSDIIQAIKF
                     NDKNMVIKICEAVQAASPVDGYVSPIPWDMPGYTNQVIMASGAFVSGSSIELSADAPM
                     RPPYIAYIQGGLFYDHVKLGVMLSLEKMMEFNEIKNKIFN"
     gene            complement(13828..14451)
                     /gene="lexA"
                     /locus_tag="PLB33_03740"
     CDS             complement(13828..14451)
                     /gene="lexA"
                     /locus_tag="PLB33_03740"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOG62666.1"
                     /translation="MSYEGLSDKQVKILQYIKDELTLRGYPPSIREICKAVGLNSTSS
                     VHAHLNTLEEKGYIKKGTNKRRALELIDLDDICCDMPKKEIINLPIIGTVTAGSPILA
                     VENIDDTLPISIDFVGNKESYVLKVKGDSMIEAGILHGDYVIVNSQRNAKNGDIVVAL
                     IGDEATVKTFYKEKDHIRLQPQNSSMDPILIKEPYILGVVKAVVRKY"
     gene            14681..14983
                     /locus_tag="PLB33_03745"
     CDS             14681..14983
                     /locus_tag="PLB33_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG62667.1"
                     /translation="MIILNKYKVSDSKKFKRFMFLSILLISIITITSITTIKAYSKDI
                     PQYDYVRVQHGDTLWSIAKDYAGSKDIRQVVYEISELNKIHNNLIHIGDIIKIPLN"
     gene            complement(14986..15966)
                     /locus_tag="PLB33_03750"
     CDS             complement(14986..15966)
                     /locus_tag="PLB33_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOG62668.1"
                     /translation="MYINYNDLCPSPLSDFLEYSIAIKGRADKTVSQYFLDLRTFYRF
                     LVIKFKLSPDVENFEEIDISPVTLDIIKKVKIMDLHSFIAYTDRERKNSNRTKARKMS
                     ALRSYFKYLHTIVKLIPENPALELETPKNKSRLPVALTLDESRSLLSSISGSNEKRDY
                     AIIMLFLNCGLRLSELISIDIDKIKGDTLTVIGKGNKERTIYLNDVCVKAVEDYLEVR
                     QEPKKGHEKALFISNRKTRFSQKGIQHMLDKYLTEAGLSGKGYSPHKLRHTAATLMYQ
                     YGHVDIRALQEILGHESISTTQIYTHINKELLRDAVKQNPLNDMNDDESL"
     gene            complement(16932..17585)
                     /locus_tag="PLB33_03755"
     CDS             complement(16932..17585)
                     /locus_tag="PLB33_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62669.1"
                     /translation="MKDSGCKKREYRDNPNVINNINTCTDTKNSNNLATGNSCTPEEL
                     IRNGGFEEVGIQTVFANWNDETEGLDINIRPSTSITYEGRQSANFDIGPVPGNTTDRA
                     TLTQTVSVNPGCYLTLSFAERLAEKPVDIELYFSAEVFYISGSNEVELIKIEKIAPRA
                     VTVESSAFSYHEKTSDIIVPQNVNFVIVRFTVSINTINASNNDTDLYLDGVSLRASR"
     gene            complement(17751..18536)
                     /locus_tag="PLB33_03760"
     CDS             complement(17751..18536)
                     /locus_tag="PLB33_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOG62670.1"
                     /translation="MSRNFTNEELSKMLYEALKIQKPIDVISDIDNEVTVEDAYDIQL
                     LNVKRALEDGEIVTGKKIGLTSKGMQELLGITEPDYGHLYKSTEVKNGGTVDTKTLIR
                     PKVEGEVAFVLKEDLKGPGITVEDVYKATDYICASIEIVDSRIKDWKIKLVDTIADNG
                     SSALYILNDKKVKIDDVSLPDIRMTLYKNGEVVNSGTGKDVLGNPAISVAWLANKLCE
                     YGITLKKGEIILSGAITASPEAFKGDMFECVFPELGNVKVEFK"
     gene            complement(18594..20198)
                     /locus_tag="PLB33_03765"
     CDS             complement(18594..20198)
                     /locus_tag="PLB33_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOG62671.1"
                     /translation="MNFNPAEYKYPSRRKVVYGYNGMVATSQPLAAQAGLEILKNGGN
                     AVDAAIAAAACLTIVEPTSNGLGSDAFALVWFNNKLHGLNGSGYSPYSISIEKLKERG
                     FTEIPKYGIIPIMVPGAPAAWVELSQKFGKLSLIEVLQPAINYAKYGFPVSVTVKKAW
                     DNAYKIYKKENKEEFKYWFDTFTYKGRTPNYGELWKLPYHAETLESIGKTHGNSYYKG
                     AIADKIDSFFKKYDGYLRKEDLEDYKTDWVEPISVNYRGYDVYEIPPNGQGLVALMAL
                     NILKNFDFTYKDKSDTYHKQIEAIKLSFDDGQKYITDIRYMKADIKQLLSDDYGRDKG
                     SLITDNALNLKETDPNSSGTVYLCTADGEGNMVSFIQSNYTGFGSGIVIPESGIALNN
                     RLHNFNYVNQHINCLMPHKKTYHTIIPGFLMKDGKALGPFGVMGAFMQPQGHVQVVSN
                     LIDFHMNPQEAIDAPRWQWIKGMTVEVEQDVPKYIINELINRGHDVKIQLNESSFGRG
                     QMILRTKEGTLCGGTEKRADGHIALW"
     gene            complement(20314..21849)
                     /gene="rny"
                     /locus_tag="PLB33_03770"
     CDS             complement(20314..21849)
                     /gene="rny"
                     /locus_tag="PLB33_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOG62672.1"
                     /translation="MFEEIIVGIICAIIGIIIGFFIRKNISESKIGSAELEAKRIIED
                     ANQKAETRKKEILVEAKDEAHRLRNEVERENKERRNEILRAEKRLIKKEEMLDSKSLV
                     VEKKDELLQKKLKEIEITQEKLDAIHQKQIEELEKISGLSTEEAKSILINNIKKEAEQ
                     EAAVAVKEIEKESKETAERKAREIITYAIQKCSADHVAETTVSVVDLPNDEMKGRIIG
                     REGRNIRALEQLTGIDIIIDDTPEAVVISGFDPIRREVARLALEKLIADGRIHPARIE
                     EMVDKAKKEVDQIIKDEGEQATLDTGIHGLHPELVKLLGRLKFRTSYGQNVLKHSIEV
                     SYLAGIMAAELGADVKVAKRAGLLHDIGKAVDHEVEGPHVTIGEDLAKKYKESKAVVH
                     AIAAHHGDTEPQTIEAVLVQAADAISAARPGARRETLETYIKRLEKLEEISNSFEGVE
                     KSFAIQAGREVRIMVKPEVTSDASIVLIAKDIVKKIEEEMEYPGQIKVNVIREIRATE
                     YAK"
BASE COUNT         7370 a         3569 c         3915 g         7156 t
ORIGIN      
        1 aatataaatg ggatgatgta ttgttggatg ctaaattatt tcaaagaatt gccttaatca
       61 tcccgggact gttgatatat atgtttgctt cattctacga taatttacag gatattatac
      121 aaaaagttgc aatcatatat atattagtta taatatcctt ggtttttaga tctctattgg
      181 ctgcaataga cagcatatac aagcaaaatc ctgtttcaaa agaaagaccg ataaaaggtc
      241 ttttacagat aattgaaatt gctatttatg tggtggtagg tatagctgtt atttcatttt
      301 taatcgataa aaatcctata tatttgttaa gcggagtggg agctgttact gctgttgttt
      361 cattgatatt taaggatata ataattggtt ttgtttcagg aatccagctt gtttggaatg
      421 atatgctgcg aataggagat tgggtggaaa tgcctaagta taacactgat ggcgatgtat
      481 tggatattac attatattcg gttaaaatac aaaactggga caaaacaata tcaacaatac
      541 ctacatctgc attcattact gattcgttta aaaattggaa gggaatgaga gaatacggtg
      601 gaagaagggt taaaaggtca tttctaattg atttgaatac tataaaaata tgctccgatg
      661 aaatggtaga aagatttaaa aaaatagatt tcattagtga gtatatggaa gaaatcacta
      721 aggaaattga caattacaac aaagaaaaca ttataaatac cgaactgcac ataaacggta
      781 aacaattaac taatttgaga gttttcaggg cttatttaac aaactatttg aaaaacacta
      841 tagaacttag gccgagcagc actaccattg tcagacaatt agctccaaca gaaaacggta
      901 taccaattga aatatattta tttgttgcag atacaaggtg ggttaactat gaaacaatac
      961 aagcagatgt tttcgaccat atattggctg taatagatat ttttgaactt aggatgtttc
     1021 agaatcctac cggtaatgat ataaaagaat taatgaagaa ataaatctca gcaaataaac
     1081 tgtcgagatt ttatttctta tattgacaat catctatgta atgaatataa taatatatag
     1141 atggttatct atatattatt ataagaggat ataaaatggt tataatcagt tggctgaaca
     1201 gacagctgct caaaatggag tggctatttg aattaattag gttattggtt gaaaatgtat
     1261 ttggactaaa catagacgat agacttgggg gaagtataca tttctttata tatgatgtaa
     1321 taaaaatttt tatattgctt tcagtactta tttttaccat ttcatatatt caaagttatt
     1381 tcccgcctga aagaacaaag aaaatactag gaagaataaa aggtgtacct gctaatattc
     1441 tgggagcttt acttggaaca ataacacctt tttgctcatg ctcatcaatt cctattttta
     1501 taggttttac aagcgctgga ctgccgctcg gagtaacttt ttcattttta atatcttcac
     1561 cgcttgttga tttggcatct ataattttat tggccagcat ctttaattgg agaacagcaa
     1621 ttgcatatgt tgtagtgggg cttttgctgg ctgttgtttc aggcacaata ataagtaaat
     1681 taaagtttga agaatatgtg gagtcttatg tttttggaaa aagctcgctt gatttagaac
     1741 aagaagaatt aacggttaac gataggattg aattttcaaa aaatcaagta tcggacatca
     1801 taaaaagagt atggaaatat atacttatag gtgtggggat aggagcagca attcataact
     1861 ggattccgga gtctttaata acagccatac tggggcagga taaatggtat tctgtaataa
     1921 tagctgcagt tgtcggtatt ccaatgtatg cggatatttt tggaacactt cccatagctg
     1981 aagctttgat tgcaaaggga gtgggattag gtaccgtttt aacctttatg atgggtgtaa
     2041 cagccctttc attgccatcc atgattatgt taaaacaagt tataaaacct agactcttat
     2101 ggacatttat cgggcttgta tctgcaggaa taataataat aggatatgca tttaatgcca
     2161 tcggatatat gttcatttaa aatataagga gggtaatatg attattaaaa tattgggttc
     2221 aggatgcaaa aattgtatta atttaaagga aaatacagaa aaagctttaa aggaaacagg
     2281 aattgatgca gaaataatta aagtcgaaaa tatgaaggat attgcaagct atggcgtgat
     2341 gcgaactcct gcattggtta ttgacgaaaa ggtggtttca tacggaaagg ttttaaagcc
     2401 gggtgatatt gcaggactat tggagaaata taaataatat ttggaaggtg acagggtgaa
     2461 aaaaaattat gagcaagcag cattattgtt tagggcatta agcgacccta atcggcttgc
     2521 tattatagat atgattaaga ctgaagaaaa atgtgcatgc aaaatattag aagaattgca
     2581 aataacccaa cctaccttat ctcatcatat gaaaatattg tgtgattcag gcttggttaa
     2641 ttctaggcgt gaaggtaaat ggatgcatta ctctattaat aaagagttgt ttgaagaagt
     2701 aaaacagtta tttgatttct aaatttaatt aataataaat ataaaagggg taccctatgc
     2761 atattaaaaa aaataattca tgttgttctt gctctgatgg ttgctgcggt tcatcagcta
     2821 agcctgagat taataagagg gaaattgtta tagatttttt gtatcttgat gtaactgtat
     2881 gtgaaaggtg tcaaggtacg gataaaagtc ttgacgaggc tattggtgaa gtggctaata
     2941 ttcttgcagc aacaggcata gatgtaaaag taaataaaat aaatgttcta aatgaggagc
     3001 ttgcaataaa gcataaattt ttgacttcac ctacaatacg cataaacggc aaggatatcc
     3061 agatggatta taaagaaagt ctttgtgagt cctgcggaga tttatgcgga gatgaggttg
     3121 attgccgtgt ttggatttat cagggaaaag aatattcaag cccgccaaag gcaatgataa
     3181 ttgaagcaat attaaaagaa gtatatggag aaaaatcaga aaaacaggag aagaaggaat
     3241 acgtaattcc tgaaaattta agaaaattct ataagtctag aagatctaaa caaaattact
     3301 cttaatatgc aatatatgat aaaggcggta aggtataacc ttaccgcnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnngct tctaccaatt ctacatcttc atatctttct
     3481 tggaagcttt ttaatgcata ttggttggta aacaataaaa caggtctttc ataatgatcg
     3541 aaaacaagca tacagcgtga attctgatat ctttttaaac tttccgggtt atctgtttta
     3601 acccatcttg caacagtaaa ctctatgttt tccatgcgaa tatccgtatt atattcgtta
     3661 actaaacgga atttaaaaac ttcaaactgt aaaactccta cagcccctaa tattacttca
     3721 ttgtattcat tcctgtaaac ttgaattgca ccttcctgag ctaattgctc aacacctttt
     3781 tgaaagtttt tccccttaag agaatttata gggcttacgc ggctgaacaa ttcaggcggg
     3841 aaggtgggca agggctcaaa aaatattaaa tctctgccgt ttgtaagagt atcgccaatt
     3901 tggaagtttc cggaatcata aatacctatt acgtcgcctg catatgcagt atccacagtt
     3961 tcacgctcat ttgccatgag atgtgtggat tggcttaatt tcatttgttt tcctgttctt
     4021 gtaagggtaa cattcattcc tcttacaaaa gttccggagc agattcttaa aaaagcaagt
     4081 ctgtcgcggt ggttagggtc catatttgct tggattttaa aaataaaacc cgtaaataca
     4141 tcatccgtcg gatggacttc ccctgtagtt gtttttcttg atgatggggg aggagacata
     4201 ataagaaaat gctttaaaaa ttctgttact ccaaagtctg ctaaggcaga gccgaagaat
     4261 acaggtgata atttgccttt tctgattaaa tctatatcaa attcattacc tgcaccgtca
     4321 agaagctcca tggcatttct caaatttgtc aaattggaag gaccgatttt tccgtctaat
     4381 ttgtttgatt taacatcttc ttcattgaga ttaataattt ctttcttttt gtataagtta
     4441 actacatttc tttgcctgtc ataaattcct tcaaattctt tgccgcatcc gataggccat
     4501 gttacagcaa cagaaggaag tcccagtaca tcctctaatt cctgcattaa ttccagcggg
     4561 tcctttgctt cacggtctaa tttgtttata aatgtgaata ttggaattcc tctcatgctg
     4621 caaaccttaa acagtttctt tgtttgcgct tctatacctt ttgcagcatc tataaccatt
     4681 acggcactgt caacagaggt taaaatacga taagtatctt caccaaagtc attgtgtccg
     4741 ggagtatcca taattgaaat tactttatca tcatattcaa actgcataac tgaagaagta
     4801 acagaaatgc cgcgctgttt ttctatttcc atccagtctg atttggcata tctggcattt
     4861 tttcttgctc gaaccgtacc tgcctgcctg attgctcctc cgtggagcag gagcttttcg
     4921 gtcaacgttg tttttcctgc atcagggtga gatattatac caaatattcg tcgtttgata
     4981 atcaaatcta aattattcat ttttttcctt tctagccggt aataattaat ctttaatatt
     5041 ctacactatt tcgacaaaaa tataaagaaa tatttacaag taattttgat tcgcaaggat
     5101 ggtacactaa tactgaaatt tagattcaat agtatcttgt ataaaggctg agggaatttt
     5161 tttattgaac aaaaattcgt aaaatgatat aatattcaag atgctaaacg tttagattag
     5221 aaagttggag atagatggac gaaagaaata ttgatattat aacagaagat ataaaattag
     5281 acggcgataa tttattagaa ttaaagaaca ataatgagca attcttcata atgaagggca
     5341 gaagaaaaac ttatcatata ttgacatacg gctgccaaat gaatgaacac gactctgaaa
     5401 aaatatccgg tatgctgaca tccatcggtt atgaagagac tgatgatgaa aaaagtgcag
     5461 accttgtaat ttttaataca tgtttaataa gggaaaatgc ggaacttaaa gtatttggca
     5521 aacttggtga agtaaaagga cttaagcgaa ataaaccgga tatgctggtt gcagtttgcg
     5581 gctgtatgat gcaaaaacaa gaaataagag aaaaactgtt aaagaaattt tcatttgtag
     5641 atattatttt tggaacaaat acaattcacg agcttccttt gttaatatac aatgttgaaa
     5701 tcaacaaaag aaaaagtatg ggtattgttg ataataccga gtcaatatat gaaaatatgc
     5761 ccaagcaaag aaagtataag tacaaagcat tggtaaacat aacatacggc tgtaataatt
     5821 tctgcactta ctgcgtagtt ccatatgtaa gaggcagaga aaaatcaaga gagccgaatg
     5881 aaataattga tgaaattaaa acattggcag aagatggctg caaagaagta actcttcttg
     5941 gacaaaatgt taattcttac ggaagcaatt tggataaaag ggttacattt gcagagcttc
     6001 tttatgaaat taataaaatt gaaggtattg agcgcatacg ctttatgaca tcccacccga
     6061 aggatttaac ggatgatttg ataaaggcaa ttaaagaatg tgataaggta tgcaaccacg
     6121 ttcatttgcc tgttcaatcg ggaagcaatg atgtgctaaa tcgcatgaac cgcaaataca
     6181 ctagggagca ttatttaaac ttggttgaaa aattaaaaac agctgtacct gacattgcaa
     6241 taacaacaga tataattgta ggattcccgg gagaaacaga ggaagatttc ttggatacta
     6301 tagatattgt taaaaaggtc gagtatgatt ctgcttttac ttttctttat tcgataagag
     6361 agggtacgaa ggcagccaaa atggagaacc aagtgccgga taatgtgaaa catgaacgtt
     6421 ttgaaaggct tttggaagtt ttgtacccga taatactgga taagaataaa cagtgtatcg
     6481 gtaaagtatt tcccgtattg gttgaatctt tcgagaacgg taaattaaca ggcaggactg
     6541 agcatttcag attagttcat ttcaatggaa gtaaggattt aatcggtaaa attgtaaatg
     6601 taaaaataac gaatgtaaaa actttccaca ctgagggaga aatagtcgag taataaggta
     6661 ttaatagaca atggtagaag gggattgtaa atggcaaagt atacacctat gatggagcaa
     6721 tatcttgcaa ttaaagagca atataaagac tgtattcttt tgtacaggct tggagatttt
     6781 tatgaaatgt tttttgatga tgctattaca gcttcaaggg tattagagat tgcacttacg
     6841 ggaagagatt gcggacaaga agaaagggct ccgatgtgcg gtgtcccgca tcatgccgtt
     6901 gacggatata ttgcaaagct tatagaaaac ggctacaagg ttgcaatatg cgagcagctt
     6961 gaagaccctt cggatgccaa aggaattgta aaacgcgatg tagtaagaat aataactccc
     7021 ggaacaataa tcgagcaagg tatgctggat gaaaaaagca acaattattt gtgcactata
     7081 tatttggaca agggttttgg tatgtcttac gtggacattt caacgggaga gctctatgta
     7141 actgaaaaca tatcgttaaa tgattttttg aaaaatagca ctcacgattt attaatagaa
     7201 gaaatcaaca aaataacccc gtcagaaatt atatccaaca accatatcgg taatgaagtt
     7261 atagattcaa tgatatattt aagcaatgta ttgagcttag atgactataa gcaaatagtt
     7321 ttgaagcatt ttaaggtact atcattggac tcattagggc ttaaagagaa ttaccatgcg
     7381 gtaatatctc ttggaatgct tttaaattat ttaaatgaaa ctcaaaaaac ttcactcgac
     7441 cacattaata agcttcattt ttatacggtg ggggaatacc tgcagattga tgcaaacaca
     7501 agaaaaaatt tggagcttat tgaaactatt agagggaaaa aaggccccgg aacactctac
     7561 catgttgttg acaacaccat gactgccatg gggggaaggc ttcttaaaaa atggattgaa
     7621 gagcctctta aaaatattaa gcatatttat ttaagattgg atgcagttga agaattatat
     7681 gaaaatataa tgacttctaa taatattaaa gaatatttaa aaagtgttta tgatatagaa
     7741 aggcttataa gccgaattgt atacggctct tgtaacggaa gagatttaaa ttctttaaag
     7801 caatccctct caaatttacc ggatttaaaa tcagaattaa atgagctttc ttctcaaatg
     7861 tttgccgata tttattcaag ttttgatgag cttaaggata tttttgattt aattgatgcg
     7921 tcgattgtag aagagcctcc catatcaatt aaagagggag gaataataaa aacaggctat
     7981 aatgaagagt tggatgaatt aagggaaatt acggtaaatg gtaaggattg gatttccaac
     8041 cttcaagcaa aagaaagaga agcaaccggt attaagaatt taaaaatcgg ttttaacaag
     8101 gttttcggct actatttgga ggttacaaaa tcatatttaa aagatgttcc cgataattac
     8161 atacgaaagc agactttagc caactgcgaa agatatgtaa cccccgaact taaagaaatg
     8221 gaagctaaaa ttttgaatgc cgatgaacaa ataatgaaac tggaatatga attgttcttg
     8281 gaaataagag agttcataag caggcatgtg aaaagaattc aagaaacagc atataatatt
     8341 gcagtaatag atgcaattaa ttcacttgca attgctgctg ttaaaaataa ttatgtgcgg
     8401 cctgaaatga acaagaaagg ttttataaaa attaccgacg gaagacatcc tgttattgaa
     8461 agaattatga aaaacgagat gtttgttcca aatgacacat acatagataa caaggaatac
     8521 cgcatgtcaa ttatcacagg tccgaatatg gcaggcaaat ccacttatat gaggcaggtt
     8581 gcattaattg cactaatgtc tcatataggc agttttgttc ccgctaaaaa ggcggaaatc
     8641 tgcataattg ataaaatatt tactcgtgta ggagcatcgg atgacctctc acagggacaa
     8701 agtaccttca tggtagaaat gagcgaggtt tcaaatattt tgaagaatgc tacttctgac
     8761 agccttttga tattggatga aatcggaaga ggtacaagca cctatgacgg gttaagcatc
     8821 gcttggagtg tagtagaata tattacgaag aagctcaagg caaaaacatt gtttgcaact
     8881 cattatcacg aattgtccga gcttgagagc aagcttaaga gcgttaaaaa ctataaaata
     8941 ttaattaaag aagaacatga tgaaataaca ttcttaagaa aaataactga aggcagcatt
     9001 gacagaagtt atggaataca agttgcaaat ttggcaaatc ttcctgaaga agtaattgaa
     9061 agagcaaaag aaatattaat tcagcttgaa gaaagcgata taaacaagcc ttttgctagg
     9121 aaaaaaaaca gggtgaacga taatttccaa gtttccatgt tccaggctaa ccctgcagag
     9181 aatgaaataa acaagaaata caaagagctg gcagaaaata ttaagaatat agatattgaa
     9241 aatataacac ctatgcaatc atttaaattg cttaatgata taatagaaaa aactaaacgg
     9301 atagatacat aacgaaagga atgtcttaca tggctataat aaatttattg gacaatgaaa
     9361 ccattaataa aatagctgcc ggagaggtta ttgaaaaccc aaagtctata gtaaaggaat
     9421 tagtcgaaaa ttcaattgat gccggtgccg atgaaataac gattgaaatt aaaaacggcg
     9481 gcaaaagctt tatacgagta accgataacg ggaagggaat tgaaaaagac caggttgaaa
     9541 atgcctttaa aaggcattgt accagcaaga tttcttcttc agacgatttg aattcgcttt
     9601 ttactctggg atttagaggt gaagccttgg caagcattgc tgccgtgtcc aaggttgaag
     9661 ttataacaag gacaaatgat gatatctatg gcattaaaat gactattgaa ggcggaaaaa
     9721 tcatttctaa ggaagatatc ggctgtcctg tcggaacaac aataaccgta acagaccttt
     9781 tttacaatgt gcctgccagg aagaaattct taaaaagcga tatttccgaa agctcaaata
     9841 tcaatgaaat tgttgtatcc ctggctctta gcaaggagaa tatttcattt aaatatataa
     9901 acaataataa tgttgcctac agaactccaa agacaaataa tctgctaaat actatttcaa
     9961 gtctttatga aaaagatctt tacaattcgc ttttaaagat agattataaa agcgatatta
    10021 taagtattac gggatataca acaaatttaa actattacag gggtaataga aagcagcaga
    10081 ttgtttttgt taacggaagg gttataaagc ataggaggat gagttatttc attgaagaag
    10141 catataacac tcttcttccc aaaaataaat atccggcatg ctttattaaa attgaaatag
    10201 ctcctgcatt ggttgatgta aatgttcatc ctgcaaagac ggaagtgcgc tttcaaaatg
    10261 aagaaatggt attaaatgaa ataaaaaatg ctgtttattc tgccttgcac tccaagagta
    10321 ttatgaagga agtaaaaggg gaaaccctct tacatgattc aaaagatata aataacttaa
    10381 gaatatttga tagaagttca ggtaaatctg tcatcttagg cggtttagaa aacaaattca
    10441 cggaagaaac agaagaaaca aagaatatta ggattacgga agttgaagtt tcaaataaag
    10501 atattgccgt aatggagccg cttaaacctt atgataaatt tgaatttaag caacaaacat
    10561 tcataaaggc agaaaatgat gaagataagc tgcctgagtt atacgttata ggcatagtgc
    10621 tggatacata tataatttgc gaaaacccgg aaaaaacatg tatattcatg atagaccagc
    10681 atgcggcaca tgaaagaata aactacgaga aatttttaaa tcaatataat tcaagagaaa
    10741 tcttaataca ggaaatagta ccggaagtga ttaatttatc ctatgatgat tacgagacag
    10801 caatgtcgaa tgtagaaata tttgaatatt tgggaatttc cgttgaaaac tttggaaata
    10861 atgctatact gataaataac ataccaacta tatttaagga ttcaaatttg aaggatgtat
    10921 tttatgcaat cctggataac ctaaaagata taactaaatc aaatttagat tttgttatgg
    10981 aaaaaataat aaaagaggca tgcgtcaagt ccgtgaagtc cggagacagg cttcatatgt
    11041 ctgaagtaag agcattgtta aatgacctta gaatgacaga taacccttac acttgtcctc
    11101 acggaaggcc tgtaattatt aaaatgacca ggtacgaaat tgaaaaaatg tttgaaagaa
    11161 ttcaaagtta ggaaagcaaa atgaataaaa taattgtaat tgtaggtcct acggctgtcg
    11221 gcaaaacata tatatccgta gagcttgcaa aaaagttaaa tactgagatt atatcagccg
    11281 attcaatgca gatatataaa gggatgaata ttggaactgc aaaaatcacc gaggaagaaa
    11341 aacaaggtat cattcatcat atgatagata ttataaatcc tgatgaagag tattcggtgt
    11401 cagaatttaa atatgatgcc gaaaaaatta ttgacaggct tttgagtgaa aacagaattc
    11461 ccataattgt aggggggagc ggcctgtatg taaattcttt gatatatgat ttagactttg
    11521 gcaataccaa gtccaataaa aaattaagag aatactacac ttattattat aaggaacatg
    11581 gggaggatgc tctgtatgac aaattactga aaattgaccc tgttgctgct gaaaaaattc
    11641 ataaaaataa tataaagaga attattagag ccctggaagt gtatgatatt acaggagtta
    11701 agttttcaga gctaaacacg gacataagaa agaaaagcaa taaatatgac tgtatattga
    11761 ttggtctttc aatggaaaga aaagtccttt atgaaagaat taatcaaaga gttgatgaaa
    11821 tgctttctaa gggtcttgtt gatgaggtta gctcacttat taaaaaggga tacggtaaga
    11881 atttagtatc catgagggga attggatata aagaaattat tgaatatctt gaaggtaata
    11941 ctgattatga ggaagcagtc aatactttaa aacaaaatac aagaagattt gcaaaaaggc
    12001 agtatacttg gttcctgaaa gatgaaaacg tcaaatggtt cagcatggat aacccaagcg
    12061 aaattgacaa aactttggat agtatagttg aatatataag cacgagaatt gatagtatta
    12121 gtaaccaatg agataaataa taataaaaat atacgctttc gcttaatttt attattatag
    12181 taatgaagta ataacatata tgcaaattta taggagaagg gaatgaatat tattaattta
    12241 caagatactt ttttaaacaa agtaagaaaa gaaaaaataa caataacgct ttatttaaac
    12301 aacggtttca agctgacggg aactgtctta ggttttgata attatgtaat ttttatcgaa
    12361 acagataaag gtcagcaact ggtttacaag catgcaataa catcaatttt gccgtttaaa
    12421 caggtacgac tgttttttga cgagccagag gagcaataat gttaacagaa aataaattat
    12481 gtaaacagtt tggcataagc gaagctgttt ataattatgt aacaaaaatt gaaaaagaaa
    12541 ttaagaagaa tcattttgaa aaaatagata aaattagaga gcttaatcaa tataaagtta
    12601 tccatgcaat gcaggagaac aagcttgatt atacaaactt ttactggaat acaggctacg
    12661 gttatggaga tgtcggaaga gaaaaggttg aaaaaatata tgcatccgtg tttaagaccg
    12721 aagatgcatt ggtaagacct tccatagctt caggaaccca tgccctgtac ttgacattgt
    12781 caggcatatt gcagcacggt gatgaagtaa tttccatatc cggacgacca tacgatactt
    12841 tgcttacagt attgggcgtg caaggaaagg aacctaataa tctcagtgaa gcaggaataa
    12901 tttataagga aattcctctt tataaaaatg atatcgactg ggaaagtgca ataaaagaag
    12961 tttcaatgaa aacaaagctt ttaatgattc agcgctcaac aggctacagc ttccgccctg
    13021 cgcttacaat cgaaaaaata aaaaatgcaa tagaaagaat tcgccaagta tatccaaata
    13081 tatatattat ggtagataat tgctacggag aatttgtaga cgaaatggag ccttcggatg
    13141 taggggcaga tgtagttgta ggctcgctta taaaaaatcc cggaggggga gttgcattat
    13201 cgggaggcta tgtggttgga aaaaaagtat taattgacag aatagccaac cgtcttacag
    13261 ctccgggagt cggaaaagag ataggactta cattcggaac cacaagaaac acgcttcaag
    13321 gtttgttttt cgcaccacac attgtgagcg aatcattaaa aggcgcactc ttgttcggcg
    13381 gggtttactc ttctctcgga tttgaaataa ctcctacagt agaagaggac agaagcgata
    13441 ttatacaggc aattaaattt aacgacaaaa acatggtaat taaaatttgc gaggctgttc
    13501 aagctgcatc tccggtggac ggttatgtgt ctccaatacc atgggatatg cccggctata
    13561 caaatcaggt aataatggct tcgggagcat ttgtttccgg ctcatccata gagcttagcg
    13621 cagatgcacc aatgagacct ccttatatag catatattca gggcggcttg ttttacgacc
    13681 atgtcaagtt gggagtaatg ctatcacttg aaaagatgat ggaatttaat gagataaaaa
    13741 ataaaatatt caattaaaaa tatggggaca ttccttatac tacaaagact atctttatag
    13801 taaaggcgtg tccccatacc tatgacatta atattttctg acaacagctt taacaacacc
    13861 caatatatag ggctccttta ttaaaatcgg gtccatggaa ctgttttgag gctgcaatcg
    13921 aatgtggtct ttttctttat agaaggtttt aactgttgct tcatctccga ttaatgcaac
    13981 aactatatct ccatttttag cattgcgctg ggaattaact attacataat caccgtgcaa
    14041 aattcctgcc tcaatcatac tgtctccctt tacttttaaa acatatgatt ccttattacc
    14101 tacaaaatca atcgaaatag gcagggtgtc atcaatgttt tcaacagcta atataggaga
    14161 gccggcagtt acggttccta taatcggtaa gtttataatt tctttttttg gcatgtcgca
    14221 gcatatatca tcaagatcaa ttaattccaa agcccttctt ttgtttgttc ctttttttat
    14281 gtatcctttt tcttctagtg tatttaaatg ggcatgaacg gaagaagtag agtttaaacc
    14341 tacagctttg caaatttcac gaatagacgg gggataaccc cttaaagtta actcatcttt
    14401 tatatattgc aatattttaa cttgtttatc gcttaagcct tcataactca taattccacc
    14461 tctttataaa attattttag actcccggga gtacctattc gcttcgtccg gatagaacct
    14521 atctgaagca tcgatttaag ctgtctgatt gagtactttt ctatatttta acatagtttg
    14581 acaagtaaag caaacatttg tttgaaaaaa tgtaaaaaat tgttgacatc gaactaatgt
    14641 tctgttagaa tgaaaacaga aaatatgttc gaggtgcctt atgataatat taaataaata
    14701 taaagtaagt gattcgaaaa aatttaaaag atttatgttc ctgtccattt tgcttataag
    14761 tattattact attacctcaa ttactacaat aaaagcatat tcaaaagata tacctcaata
    14821 tgattacgta agagttcaac acggagatac cttatggtca atagcaaaag attatgccgg
    14881 cagtaaagat ataagacaag tagtatacga aatatccgag cttaacaaga tacataataa
    14941 cctgattcat atcggcgaca taattaaaat tcccctaaat taaacttaaa ggctctcatc
    15001 atcattcata tcatttaagg gattttgttt taccgcatca cggagcaatt ccttgtttat
    15061 atgagtgtaa atttgcgttg ttgaaatact ttcgtgtccc agtatttctt gcagcgctct
    15121 aatatctaca tggccatatt gatacataag ggttgctgcc gtatggcgaa gcttgtgagg
    15181 ggagtatcct tttcctgata aacctgcttc agttaagtat ttatccaaca tatgctgaat
    15241 gcctttttga gaaaaacggg tttttcgatt acttataaaa agagcttttt catgaccttt
    15301 tttcggctct tgtcttacct ccaagtaatc ttctacagct tttacacata catcattcag
    15361 gtaaattgtt ctttccttgt tgccttttcc gattacagtc aaggtgtctc ccttaatttt
    15421 atcgatatcg atgcttataa gctctgataa tcgcaggccg caattcaaaa ataacataat
    15481 aatggcatag tcgcgttttt cattggagcc tgatattgat gataataaac ttcttgattc
    15541 atccagcgtt aatgctaccg gcagacggct tttgtttttt ggagtttcta attcaagagc
    15601 cggattttca ggtataagct ttactatggt atgtaaatat ttaaagtatg acctaagtgc
    15661 tgacattttt cgagcttttg tacggtttga attttttctt tccctgtccg tataggcaat
    15721 aaaagaatga aggtccataa tttttacttt ttttataata tccaaggtaa caggggagat
    15781 atcgatttct tcaaaatttt caacatcagg acttaatttg aatttgatta ctagaaatct
    15841 ataaaaggta cgaaggtcaa gaaagtattg actaacagtt ttatccgctc tgcctttaat
    15901 agcaattgag tattctaaaa aatcactaag tggggaaggg cataggtcat tataatttat
    15961 gtacatcatt atctccttta caaaaatgtc gcaaaattct tacttaatta tatagaagaa
    16021 ttaggaaaaa gacaagcttt tttagtaaaa cctatatcat tttgattaat aaaataacac
    16081 aaaaatactt ttcttaaatg caagtgctca aatttacaag tattgtgtga attgcaatta
    16141 acttttttcg atacttcgac atataaccag tatttattca tatattcgac ccgtatatct
    16201 gcttttaata aaaaataacc acacattaat tgaaattttg agcatctcat taaacttagt
    16261 attctctgat gagcattaga ggaaagtccc caattcggac aagtcttaga ggtatgagga
    16321 atatatctgt cttatagatg atagaaatca aattttgcct gttaattaaa aacgagctat
    16381 atttaaaggg tataaatggt caaggaaaga agtttcgtgc gttaaatcgc attttaaagc
    16441 ctcgtttttt gggcatattt ttgaaatttc agccggttac ttgacttggt cgttttttgt
    16501 ctatagtgtg caagagtgaa cgaatatttt tattttttca tcttgagcat agcgtagtaa
    16561 ctattaacat tgtcattata agaataagcg tagtatctaa tacataagag aaagatccgg
    16621 tgagattttc gagctacaac aatcaggaca gaactgtaaa gaaaaaaata tccaaaccgc
    16681 aaaggtaaat tattaaaaaa tattacaaga attataaatt tgtgaattga aattataaaa
    16741 gaaaaataat caaatcgata aaaaaatcga gaagaacgtt gaaattccaa aaagaagggg
    16801 tctttcggaa aagtgcttat ttcagggata ttatgtcgct aaattagcat ttagcgacat
    16861 aataaaaaat aataatattt atataaatta acaattttgt aattattact gctattccct
    16921 tgatatcccg gtcaacgaga agctctcaat gatactccgt ccaaatataa gtctgtatca
    16981 ttattgcttg cgttgatagt attaatgctg acagtgaatc ttactattac gaaatttaca
    17041 ttttgaggta caattatgtc tgacgttttt tcatgataag aaaatgctga tgattctact
    17101 gtaacagctc ttggagctat tttttctatt ttaattaatt caacttcatt agaacctgaa
    17161 atataaaaaa cttcagcact aaaatataat tctatatcta ccggtttttc tgctaatctt
    17221 tctgcaaagc ttagcgttaa ataacatccg ggattaacac taactgtttg tgttaatgtt
    17281 gctctgtctg ttgtattccc gggtaccgga ccaatatcaa aattggcgct ttgtctgcct
    17341 tcatatgtta tagaagtact tggtcgtata tttatatcaa gaccttccgt ttcgtcattc
    17401 caattagcaa aaactgtttg aattcctact tcttcaaagc caccatttct tataagctct
    17461 tcaggagtac atgaatttcc tgttgctaaa ttattgctgt ttttcgtatc tgtgcaagta
    17521 tttatattat ttattacatt aggattatct ctatattccc ttttcttaca gcctgaatct
    17581 ttcataatgt taacgcctcc aatatataaa acaagcttaa gctcataaat ataatatgta
    17641 caacctttat aattgttaca taaataaatg tggcataaat tgtattaaat gacggagaat
    17701 caaaaaaaca tcggagctct tcagccctga tgttttgtta ttatctgtta ttatttgaat
    17761 tctaccttga cattccccag ttcaggaaaa acacattcaa acatatcacc tttaaatgct
    17821 tccggtgatg ctgtaattgc tcccgataaa attatttcgc cctttttaag agtaattccg
    17881 tattcacaca gtttatttgc aagccatgca acacttattg ccggattccc taatacatcc
    17941 ttacctgttc cgctgtttac cacttccccg tttttataaa gagtcatcct tatatcaggc
    18001 aatgatacat cgtctatttt tactttttta tcgtttaata tatacaaagc gcttgaaccg
    18061 ttgtctgcaa tagtatctac cagtttaatt ttccaatctt taattctcga atccactatt
    18121 tcaatggaag cacatatata atcagttgct ttataaacat cctccacagt tataccggga
    18181 ccctttaaat cttctttcag tacaaaagca acttcaccct ctacttttgg ccttattaac
    18241 gtttttgtgt ctactgtacc gccgtttttt acttccgtgc ttttatataa atggccgtaa
    18301 tccggctcgg taatacccag cagttcctgc atgccctttg aagtaaggcc gattttcttg
    18361 ccagttacaa tttccccgtc ttccaatgct ctcttaacat ttaacagctg aatatcataa
    18421 gcgtcctcaa cggttacttc attatcgata tcacttataa catctatagg tttctgaatt
    18481 tttaatgctt cgtaaagcat tttggataat tcttcgtttg taaaatttct agacatattt
    18541 tcctccttat atattaatat attctaatgt ccaactaatc ccccgtcccc ttgtcaccaa
    18601 agggctatgt gtccatctgc tctcttttct gttccgccgc agagagtgcc ttcttttgtc
    18661 cttagtatca tttggcctcg tccgaaagat gattcattta actgtatttt tacatcatga
    18721 cccctgttaa tcagctcatt tataatatat ttcggaacat cctgctctac ttcaacagtc
    18781 attcctttaa tccactgcca cctcggtgca tctatggctt cctgaggatt catatgaaaa
    18841 tcgattaaat tacttactac ctgcacatgt ccttgaggct gcataaatgc ccccattaca
    18901 ccaaagggac ctagagcttt tccgtccttc attaagaatc cgggtattat cgtatgatag
    18961 gttttcttat gaggcattaa acaattaatg tgttggttta cataattaaa attatgtaac
    19021 ctattgttaa gagctattcc tgattcaggt ataacaatac ccgagccaaa tccggtataa
    19081 ttgctctgaa tgaaagaaac catattccct tccccatcag ctgtacacag ataaacggtt
    19141 ccgctgctgt ttgggtcggt ttcttttaaa ttcaatgcat tatcagtaat taaacttccc
    19201 ttatctctgc cgtaatcatc cgataataat tgctttatgt ctgctttcat gtatcttata
    19261 tcggtgatat acttctgacc atcatcaaag ctaagtttaa tagcttcaat ttgcttgtga
    19321 taggtatcgc tcttatcctt gtatgtaaaa tcaaaatttt ttaatatgtt aagcgccatc
    19381 aaagctacca gtccctgacc gttgggaggt atttcatata catcgtatcc cctgtaattt
    19441 acgcttatag gctctaccca atctgtttta taatcttcta agtcttcttt tcttaaatac
    19501 ccgtcatact ttttgaaaaa ggaatctatt ttatctgcta tggcaccttt atagtatgag
    19561 ttgccgtgtg tttttcctat tgattccaat gtttcggcat gatacggaag cttccaaagc
    19621 tctccgtaat ttggtgttct ccccttatag gtgaatgtat caaaccaata tttaaactct
    19681 tctttatttt cttttttgta aattttatat gcattatccc atgctttttt tacagtaaca
    19741 gaaacgggaa atccatactt ggcataatta atggcaggct gtaagacttc tattaaagaa
    19801 agtttcccga atttttgaga taattctacc caggctgccg gagcaccggg taccattatc
    19861 ggtataatgc cgtactttgg aatttcagta aatcctcttt ctttcaattt ttcaattgaa
    19921 atgctgtagg gtgaataccc gctgccattt aaaccatgaa gcttattatt aaaccataca
    19981 agggcaaatg catcgcttcc caaaccgttc gatgtaggct caacaattgt aaggcatgct
    20041 gctgcagcta tagccgcatc aacggcattg ccgccgtttt taagtatttc aaggcctgct
    20101 tgtgctgcta atggctgtga tgtggcaacc attccgttat atccgtagac aacctttctt
    20161 cttgacggat atttatattc ggcagggtta aaattcatgt tttttttact cctttctttg
    20221 atttatcatg acaacactcc ctaaggatgc ttagaaattg tctgttatta aaaagaagta
    20281 aacaaattgt ttacttccat atttttctaa cctttacttt gcatattctg tagctctgat
    20341 ttctctaatt acgttaactt taatctgtcc gggatattcc atttcttctt ctatcttttt
    20401 aactatatcc tttgctatta aaactatgct cgcatcgctt gttacttcag gtttaaccat
    20461 tattcttact tctcttcccg cctgtattgc aaaagacttt tctacccctt caaatgaatt
    20521 tgaaatttct tctaactttt caagcctctt tatataagtc tctaatgttt ctcttcttgc
    20581 acccggtctt gcagctgata tagcatctgc tgcttggact aagactgctt ctatggtctg
    20641 tggctctgta tccccgtgat gagctgctat agcatgaact acggccttgc tttctttgta
    20701 ctttttagct aagtcttcac ctattgtaac gtggggacct tcaacttcat gatccactgc
    20761 ctttcctata tcatgcaata atcctgctcg ttttgctact tttacgtcag cgcctaattc
    20821 agcagccata atacctgcta aatatgatac ttcaatggag tgtttcaata cgttttggcc
    20881 gtaacttgtt ctgaatttca gtctgccaag taattttacc agttccgggt gaagcccgtg
    20941 gattccggta tcaagggttg cctgttcgcc ttcatcctta attatttggt ctacttcttt
    21001 ttttgcctta tcaaccatct cttcaattct tgcaggatgt atcctaccat ccgcaattaa
    21061 cttttcaagt gctaatctgg caacttctct tctaataggg tcaaatccgg atataactac
    21121 tgcttccggt gtatcatcta ttataatatc tatacctgta agttgttcca aggctcttat
    21181 atttctgcct tcccttccta ttattctgcc tttcatttcg tcattcggca ggtcgactac
    21241 agatacagtt gtttctgcta catggtctgc agaacatttc tgaatcgcat atgtaataat
    21301 ttcacgtgct tttctttctg cagtttcttt tgattccttc tcgatttctt tgacagcaac
    21361 cgcggcttcc tgttcagctt ctttttttat gttgttgatt agaatgcttt tagcttcttc
    21421 tgttgaaagt ccggatattt tctcgagttc ctcaatttgt ttttggtgaa ttgcatctaa
    21481 tttttcctgc gttatttcaa tttcttttaa cttcttttgg agcaattcgt cttttttctc
    21541 tactacaagg cttttagagt ccagcatttc ttctttttta atcagccttt tttcagccct
    21601 taaaatttcg tttcgtcttt ctttgttttc tctttcaact tcatttctta acctgtgagc
    21661 ttcgtctttt gcttcaacaa gtatttcttt ttttcttgtt tcggcttttt gatttgcgtc
    21721 ttcgattatt ctttttgctt ctaattcagc actacctatt ttgctttcag atatattttt
    21781 acgaataaaa aatcctataa ttatacctat tattgcgcag ataataccga ctattatttc
    21841 ttcgaacaat tcagcacctc caatattaaa ttttcaagct gcgaaatata tacatttttc
    21901 ctaattgcta taaaaaatgt gtatcttcaa aaacatatat tttaatcgtc ttaattagtt
    21961 ttgtaacctg aatataattt attttataat tatttataaa caatgtcaag atataatgtt
    22021 tatatttgca tatttttaca tattctaatg catatcaaag cgtgttggtt tacataattt
    22081 aaattatgta aactatggta tatagtttgt
//
LOCUS       DAOSHP010000023        21529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_1967_length_21529_cov_9.165642, whole genome shotgun sequence.
ACCESSION   DAOSHP010000023 DAOSHP010000000
VERSION     DAOSHP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21529
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1967_length_21529_cov_9.165642"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLB33_03775"
     CDS             complement(<1..332)
                     /locus_tag="PLB33_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOG62673.1"
                     /translation="MMNSVIHTNNAPAAVGPYSQGIKAGNTIYVSGQLAINPATGELY
                     KGGDIKEETRMSLMNVKAILEEAGATLKDVVKCTVFIKDMNQFGLINEAYAEVFQDTK
                     PARVCVEVA"
     gene            complement(339..707)
                     /gene="rsfS"
                     /locus_tag="PLB33_03780"
     CDS             complement(339..707)
                     /gene="rsfS"
                     /locus_tag="PLB33_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOG62674.1"
                     /translation="MIEVQKNIETILKACDNKKAYNFVVLNVSKTSSITDYFIICSGN
                     NEKQTVAIAEEVLEKGAKEGIDFFYKEGFETGRWILLETDDIIVHIFHKDERAIYDLE
                     SLWYDNNSLDVEQYGIKNWK"
     gene            complement(704..1204)
                     /gene="yqeK"
                     /locus_tag="PLB33_03785"
     CDS             complement(704..1204)
                     /gene="yqeK"
                     /locus_tag="PLB33_03785"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOG62675.1"
                     /translation="MHIEEMKKELKIELGEKLYRHSIGTMEEAEKLASVYGCDVSKAI
                     IAGLLHDCGKRICSDNLKHAKKSAELAGVKYGVKDNDILNAIMFHTTGRVNMSLLEKI
                     IYIADKIEPNRNYEGVEEIRNKAYTRLDEAIIKSLENTIEYVKNRNLVLDMESVNTLN
                     YLKEGK"
     gene            complement(1239..1856)
                     /gene="nadD"
                     /locus_tag="PLB33_03790"
     CDS             complement(1239..1856)
                     /gene="nadD"
                     /locus_tag="PLB33_03790"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOG62676.1"
                     /translation="MKKKVGIMGGSFDPIHMGHLVAANEALNIYKLDEIIFIPTGDPP
                     HKDYPNAESFHRLLMVNMAVLTNDKFSVSDIEIKNPSKSYTLSTLRELHKLYKNTEFY
                     FITGTDAVLNLPSWHQPEEVLKLCRFIAATRPGISIEEVNTKINEIKRMFGGNIEILQ
                     VPMLQISSSDIRNRIKMGVSIKYLLPDSVEQYIIKNSLYKNGQEK"
     gene            complement(1853..3289)
                     /locus_tag="PLB33_03795"
     CDS             complement(1853..3289)
                     /locus_tag="PLB33_03795"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOG62677.1"
                     /translation="MTFSDYCLLSIFYFWASSTAFSVGIGYYTIYRPIMAGMAAGLIL
                     ADIKTGMLAGAVVNIIFIDFISTGGSLKGDQCLTAILAAAASIIFKLNPLEAAAAAFP
                     FGFLGILIWKYRLKINNIFVHIYEKKYKENKAPDISLYNGLFPQLLLFMMSLAVIITA
                     FCLMFLFNKLIHFNSISVLLYYIGIILVIFSVSNVLYKIKIKYNYAIFISVFLAALVL
                     DMRAYILFILIVLSLLYLSEKKVSIVRNSFNGKLKRGDLVYSWFIWMNYSHSCYSYER
                     LMGLAFAHSMKNIIRKLYADEHEIAEAIHNHTEFFNTEPNMGTPIHGYIISLEEERML
                     NNQSFEDISYIKKGMMGISAGLGDSFTQAILAPLFISMSVMLCLDGSFYLAFIPVIIL
                     SVFILFISYNGFMNGYFQGRESMLQRIKDVKQSKIKLYFPYIFSGILGFSISKLLFNN
                     IRPYEDIFTLSIILFAAVIMFIRKRGEQ"
     gene            complement(3291..3752)
                     /locus_tag="PLB33_03800"
     CDS             complement(3291..3752)
                     /locus_tag="PLB33_03800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOG62678.1"
                     /translation="MKNVFVRIDDRLLHGQVVVSWIPYLRAKEVVIADDEYANDEFMS
                     ELIKSSGPEGVIVHVKTIDETAAYLSDDNQNKILVLLRSIEGIKKLIKKTKISSINIG
                     GVGAAQGRKRYYNSIHLSDDELNILNDIANDNIYVEIRILPKDKAIIIKGN"
     gene            complement(3761..4159)
                     /locus_tag="PLB33_03805"
     CDS             complement(3761..4159)
                     /locus_tag="PLB33_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HOG62679.1"
                     /translation="MINILLVTHGEFGKELLKSSEMIIGEVENAKSISFNHGDSFETL
                     LKKVEENIKNISDDELIVFTDMYGGSPYNAVNRAMKNRNFYHITGINFPLFIDIAVAR
                     DTYSLEEIAEKIIKNGKKSIVFVNEKFLSD"
     gene            4399..5082
                     /locus_tag="PLB33_03810"
     CDS             4399..5082
                     /locus_tag="PLB33_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62680.1"
                     /translation="MNNYRNNMPHYDSLPHNYNPMGINEHKNSNRMHLANTMPRMPGM
                     PDDMLQRPFIPETEMPLEIDGDMPALPRFPQQGIPSLIPPWLTQPEIPMMPGMQPTPG
                     MPGMPQMPGMQPTPGMPGMPQMPGMQPTPGMPGMPQMPGMQPTPGMPGMPQMPGMPQM
                     PGMPNGMPQMPGMPPDDGNMYPMMPGMPNGMPPINCEQLRELMRRMNCPMDGGGGENG
                     TVPPMNEEM"
     gene            5206..5907
                     /gene="sigK"
                     /locus_tag="PLB33_03815"
     CDS             5206..5907
                     /gene="sigK"
                     /locus_tag="PLB33_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOG62681.1"
                     /translation="MIQELLQILLPLSIFIFGYITNGSSFPHPLTKEEEACLIERANK
                     GDIEARNILIERNLRLVAHVIKKYKTVGIDQEDLISIGTIGLIKAVSTFDEKKGIRLA
                     TYAAKCIDNEVLMTIRSEKKLKKEVSLQGPLGTDKEGNNIYLIDIIKYEGEEIVGRLD
                     KEEKIKALDEILDKTLQKRERFIIEMRYGIRNKEHKTQREIAKLLGISRSYVSRIEKK
                     ALKKLHDALVNNVGN"
     gene            complement(5978..7201)
                     /locus_tag="PLB33_03820"
     CDS             complement(5978..7201)
                     /locus_tag="PLB33_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOG62682.1"
                     /translation="MLPKLLAPAGNLDKLIMAVQYGADEVYFAGKSMGMRAGSKNFTK
                     DDLKQGVEYCHKYGRKANITVNIIPHNDDLKGIEGYLKYLHKIGTDALIVADPGVVEI
                     VKQTIPDMKIHLSTQANTTNYHTANFWYKQGVNRIVLARELTFEEIETIIKNTPEDLE
                     FETFVHGAMCISYSGRCLLSSFMTGRFSNKGDCAQSCRWKYNLVEEKRPGEYYPIEET
                     GEGTFIMNSKDLCMIEHLQKFKELGIKALKIEGRNKSEYYTALTVRSYRNALDELKYP
                     ESERNTGYWKEELEKTSHRDFSYGFFFGNPYKDGQLYTHSSYIRTYDVVGIIRSYDES
                     SKIATVEQRNKFSEGDILECFGPKGKHFELKAENLTDENNNKIDSAPHPQQTVKMYIE
                     KNVEPYFILRKKLEE"
     gene            complement(7282..7926)
                     /locus_tag="PLB33_03825"
     CDS             complement(7282..7926)
                     /locus_tag="PLB33_03825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOG62683.1"
                     /translation="MNNINSSYINEYINSLIIEDSEKLNKFRDYCEKRSLPIIHKEVG
                     QFIKLVINLLNAKSIIEIGTNVGYSSIFMSKVMKDEGKVVTLERSEKFYNEALKNITD
                     FGLEKKIEVYFGDAVDILDQVEGTFDMAFIDAAKSYYRVFFDKCLKMMKPGGIILSDN
                     VLYQGMIASDELVVRRKKTLVRNLRNYLEYISHDERFVTSVLPLGDGLAVTLIK"
     gene            complement(7938..8954)
                     /gene="mltG"
                     /locus_tag="PLB33_03830"
     CDS             complement(7938..8954)
                     /gene="mltG"
                     /locus_tag="PLB33_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOG62684.1"
                     /translation="MKKIIALLCVLSIIAAAYLYLNNYIEENLEAVDLGDDSPIVVEI
                     PAGSTTNDIAEILFENNLISNIRVFKYYAEKTGSDAKLKAGTYILSKSMNADELLESL
                     IKGGSSGNTYNITVIEGLTNEDMAQSLSEQTALNYDRFIELMESPEIFNQDFEFLKDS
                     SVTNLQGYLMPDTYNIYVDSTEEDIIRVLLKQFDKFYIDEIKPRIDKTNLTFEEVINL
                     ASIVEKEALLDEERDEVAKVFLNRLSINMKLQSCATVNYANGEWKERLTLEDIEIDSP
                     YNTYIVEGLPPTPINSPGRVSIIAVLEPADVDYLYFVAKGDGSHYFSNTYDEHIDAAE
                     EYLN"
     gene            9105..9347
                     /locus_tag="PLB33_03835"
     CDS             9105..9347
                     /locus_tag="PLB33_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62685.1"
                     /translation="MDRLNKISTPDLSKVGNLDVIKLRRDGQYNLTSAINGRITPNLN
                     LTSKRSNQRMEDKSIVNNKPSQFRPILGVNFHNIKR"
     gene            complement(9473..10174)
                     /locus_tag="PLB33_03840"
     CDS             complement(9473..10174)
                     /locus_tag="PLB33_03840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOG62686.1"
                     /translation="MINNETIEAERTRVCLTIGRIAPDFTAVTTEGPITLSQYRGKWV
                     LFFSHPGDFTPVCTSEFIAFAQLQPEFEKRNVQLLGISIDSNPAHLGWLYDIFQMTGI
                     VMPFPLIADRDASIAALYGMVSPDRIYEQSVRDVFIIDPNQRIRSILIYPATNGRNMY
                     ELLRLIDALQVSTTYGVSTPANWMPGDPVIVPVASTLDEAIERGMDPESLELNCLAWW
                     YCFTDLSELTPLPNP"
     assembly_gap    10307..10406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10472..12148)
                     /locus_tag="PLB33_03845"
     CDS             complement(10472..12148)
                     /locus_tag="PLB33_03845"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOG62687.1"
                     /translation="MAEKKHKLKVIPLGGLGEIGKNITVIEYHNDIIVIDSGMAFPGD
                     EMLGIDIVIPDITYLIKNKEKVKGIILTHGHEDHIGSIPYFLKKLNIPIYGTKLTLGL
                     VENKLLEHKIEDASLNPINAGDTLKLGCFKITPIRVNHSIPDSVGFAIDTPIGTIVHT
                     GDFKIDYTPINEDVIDLGKFADIGRKGVLLAFSDSTNVERPGFTESEKTIGQKFMDIF
                     KNCDSRIIVASFASNIHRLQQVIDAAVVNKRKVAISGRSIINAIKVSLELGYLNAPQG
                     TIINVNELNSLKDDEIVILTTGSQGEPMSALTRMANNDHKKIQIKEGDLVIISANPIP
                     GNEITVSRVINMLYEKGAKVLYDALTQVHVSGHARRDELKLMLTLLKPKFFIPVHGEY
                     RHLVQHAKLANEIGIPINNTVIGSNGDVIELTSKSIVKNNTVTSGNILVDGLGVGDVG
                     NIVLRDRRLLSENGLIIVVLSTDRSTGKILAGPEIVSRGFIYVRENIDLIEESKTVVR
                     KALEKCEDTNVKEWNNIKMVIKDSLGSFIYEKIKRTPMILPIIVEVDLEV"
     gene            complement(12129..12593)
                     /locus_tag="PLB33_03850"
     CDS             complement(12129..12593)
                     /locus_tag="PLB33_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOG62688.1"
                     /translation="MADYLRTLLDKNGYKTTVQRKILYEVLSDNKDKHLSTEEIYDLI
                     KNRYPNIGIATIYRTLLLFEDIGLVYKHNFDDGFSRYEILSQNSNEIHQHHHLLCKQC
                     GKIIEVKEDLMNTLEEIIEKQYNFEILNHVVKFTGICAQCRDKENKDGRKET"
     gene            complement(12617..12859)
                     /locus_tag="PLB33_03855"
     CDS             complement(12617..12859)
                     /locus_tag="PLB33_03855"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOG62689.1"
                     /translation="MDNIIELINDEGKLEKLVVEATFHIDDILYAVLHKINSDEGMVY
                     YVEEMEDGSIVLVKVEDEEEIKEVQEIYEGIADDLI"
     gene            complement(12874..13287)
                     /gene="ruvX"
                     /locus_tag="PLB33_03860"
     CDS             complement(12874..13287)
                     /gene="ruvX"
                     /locus_tag="PLB33_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOG62690.1"
                     /translation="MDRLMGLDVGDKTIGVAVSDLLMITAQGVTTIKRTNIKNDLNEL
                     KKLIDEYQVTKIVAGIPKRLDGTIGIQGEKVLEFLEKMKKHIDLPVEFEDERFTTTMS
                     ERMLIEADVKRKKRKEVIDKLAAVQILSSYMQRTK"
     gene            complement(13295..14242)
                     /locus_tag="PLB33_03865"
     CDS             complement(13295..14242)
                     /locus_tag="PLB33_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG62691.1"
                     /translation="MKYINLFEKENVSRLCYGTLSLSSLQNLDTVENKVRLLNYAYEK
                     GINFFDTAQLYDNYNILKEFIKHKDRDKLVIASKSYAYDKNTAEYSINKALKEMNTDY
                     LDIFMLHEQDNGNNFLGHYKAVERFMKYKEQGIIKYFGISTHRVEAVKDSLKFKEIEI
                     IFPLINYKGIGIQDGTFEDMTEALKEAKENNKFIMAMKIYGGGHLIKEAEKAFKYIDS
                     LNYVDSIAIGMSTVEEIDANISYLENNELNINIKNKIKNQNRTLVIEEWCAGCGKCVK
                     KCKQNALAIVDGKCAVDMEKCVLCSYCAGECAGFYIKIV"
     gene            complement(14351..14614)
                     /locus_tag="PLB33_03870"
     CDS             complement(14351..14614)
                     /locus_tag="PLB33_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOG62692.1"
                     /translation="MDSNVNNTAKFDYSMGSLNEAREIISQVYKSLKEKGYNPNNQLI
                     GYILSGDPTYITNHNNARSLIRKIERDELLEEILSVYLNVIDI"
     gene            complement(14752..17379)
                     /gene="alaS"
                     /locus_tag="PLB33_03875"
     CDS             complement(14752..17379)
                     /gene="alaS"
                     /locus_tag="PLB33_03875"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOG62693.1"
                     /translation="MDRFSINNIRKEYLNFFKSKGHLTEQSFSLIPKNDKSLLLIGAG
                     MAPLKKYFTGAEIPPAKRMATCQKCVRTGDVENVGLTARHLTFFEMLGNFSFGDYFKN
                     EAIVWAWELLTDVLKIDKDRLWVTVYLEDDEAENIWHTKVGIPMEKIVRLGKDDNFWE
                     LEVGPSGPCSEIYYDTGEDRGCGSTDCKPGCDCNRFIEIWNLVFTQFDKDEEGVYHPL
                     PNPNIDTGMGLERVACVLQDKPTVFDVEPLVFIVNKVSEICKIPYGQNLESDVAMKVI
                     CDHSRAATFMIGDGVIPSNEGRGYVLRRLIRRAIRKGKSLGINGEFLTKTAKVVIDNW
                     KEVYPELGEKEKYILKVLSLEEERFKITIDQGLELLMKEIQKLKETNKKELDGNVAFK
                     LYDTYGFPSELTEEILKENGFSLNKEQFNAEFEEHRQMAREARKTTGASGWKDNDFTL
                     DTDKTIFTGYNKLEDISEVIAIVVGSEQVNTINEGQSGIIVLDKTPFYAEGGGQTGDS
                     GEFTADEMLAKVTDTHKFNNGIYLHDTNLVKGSISVGDTITAKVNDDQRKNTAKNHTC
                     THILHKVLREILGDHVNQAGSYVSEDRLRFDYTHFEALDKKTLKEIEKRVNEAILSDY
                     TVKTDILSMEEAKKSGAVALFDEKYEEMVRVVSVGDFSKELCGGTHIDQTAKIGMFKI
                     ISEGSIASGIRRIEAITGRAAIDYMQELDNLIDELSISMKTTKDELLSKANLLKKEAK
                     EKDKEIQRLNNEILKSNINEILDKYEEINGIKLFAIKFKDKDANTLREIADKIRDKNE
                     SCAIILASDLGDKVLFVSAVTKDLAKGGIHAGNMVKEAAVIAGGGGGGRPDFAQAGGK
                     NPEKIDEAINAVRKQIASI"
     gene            complement(17576..18004)
                     /gene="nifU"
                     /locus_tag="PLB33_03880"
     CDS             complement(17576..18004)
                     /gene="nifU"
                     /locus_tag="PLB33_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOG62694.1"
                     /translation="MYSEIVMDHFRNPRNVGHMEKPDGVGQVGNPKCGDIMKIYLKIK
                     DDIIEDVKFETFGCGSAIASSSIATELIKGKSIHEAVELTNKAVVEALGGLPPVKVHC
                     SVLAEQAVKSALYDYAKKANKVIKGLENYKPEDDEIHCQH"
     gene            complement(18020..19207)
                     /gene="nifS"
                     /locus_tag="PLB33_03885"
     CDS             complement(18020..19207)
                     /gene="nifS"
                     /locus_tag="PLB33_03885"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOG62695.1"
                     /translation="MSRKLYLDNAATTRVKKEVIDEMRQYFDILYGNPSSQLYELGRK
                     SKEAIEKARKTLADFLNAEEKEIYFTASGTESDNWALKGVAFANLNKGKNHIITTKIE
                     HHAILHTCEYLEKFGIETTYLDVDRYGLIDIEQLKESIRPETMLISVMFANNEIGTIQ
                     PIEEIGKIAKDHGILFHVDAVQALGGIRIDVNKLHIDLMSMSAHKIGGPKGIGGMYIR
                     KGTRIDNFIHGGGQERGRRAGTEGVQNIVGFGKAVEIAARDFDKHVERLTVLRNRLID
                     GIKNNIPDVVLNGHPTQRLPNNVNFSYKFVEGESILLLLDMEGISASSGSACTSGSLD
                     PSHVLLATGLDHGTAHGSIRFTLSEELTEEDIDYTVETMKKIITRLRAMSPIKNDEDL
                     HKN"
     gene            complement(19185..19631)
                     /locus_tag="PLB33_03890"
     CDS             complement(19185..19631)
                     /locus_tag="PLB33_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOG62696.1"
                     /translation="MILSTKGRYGLKAAFELSKNYGLGPVSLKKISEKYGISENYLEQ
                     LFAKLKKSGYIDTVRGPQGGYLLAKKPEEITVGMILRTLEGEIKTSDCLGKEVCSRES
                     ICATRVIFEKIEKSINDVIDNITLADMYEEQKNILTEGRNEQETLS"
     gene            complement(19775..20035)
                     /locus_tag="PLB33_03895"
     CDS             complement(19775..20035)
                     /locus_tag="PLB33_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOG62697.1"
                     /translation="MEVYIITFLHGNYAVAAFNRLQGFGVKNIHLIQTPFSIKNECDL
                     CIKTNDRKTLDIILNECRGRYPVSNVYTQINKNGSFVYKLLP"
     gene            complement(20115..20612)
                     /locus_tag="PLB33_03900"
     CDS             complement(20115..20612)
                     /locus_tag="PLB33_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62698.1"
                     /translation="MYKIFGKITDPNIENIISSKLNFDNLNVDQVYDIYVDFYNTDLE
                     EGMLNADISYEIKKEHYLFIKKIRALFENNQIRVNEFYLMGTISDLPENEINISIMKS
                     QGDKKKNVVWPCKEIFLYENQKNKLDALLISNQISEEDYESNLEFLKDELNIYENDEE
                     HKYIN"
     gene            complement(20624..>21529)
                     /locus_tag="PLB33_03905"
     CDS             complement(20624..>21529)
                     /locus_tag="PLB33_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOG62699.1"
                     /translation="ENGTIILIGATTENPYFEVNSALISRSLVFEFKRIDIEDIKNVV
                     RKSLVDKRGYGNRKVEIDEDALAHIAEYANGDIRSALNALELGVETTEEIDGIIKINI
                     DIASECIQRKKIDYDKDGDNHYDTISAFIKSMRGSDPDAAVYWLAKMIKAGEDPKFIA
                     RRIIICASEDVGNADPRALEVAVNAFKAVEIVGFPEGRIILAQAAIYIACSPKSNASI
                     IAIDKAMNAVENESHEVPAHLKDKHYKGAEKLNRGIEYKYPHDYDKFYVKQQYMPETI
                     NTKFYEPKESGYESRIKKFLEYLRQ"
BASE COUNT         6229 a         4393 c         3052 g         7755 t
ORIGIN      
        1 gccacttcta cacaaactct tgccggtttt gtatcttgga atacttcagc ataagcttca
       61 ttaattagtc cgaattgatt catatccttg ataaataccg tgcatttaac aacatccttc
      121 aatgttgctc ctgcttcttc caaaatagct ttcacattca ttaaggacat tcttgtttct
      181 tctttaatgt caccgccttt gtacagctcc cctgttgccg gatttattgc aagctgtccg
      241 gaaacatata ttgtgttgcc ggcttttatt ccttgagaat aagggcctac tgctgccggt
      301 gcattgttag tatgaataac actattcata attacctcct atttccaatt ttttattcca
      361 tattgttcaa catccaatga attgttatca taccacaatg attccaggtc ataaattgcc
      421 ctttcatcct tatgaaagat atgaacaata atgtcatcag tttctaataa aatccatctt
      481 ccggtttcaa aaccttcctt atagaaaaaa tctattcctt cctttgctcc tttttccaac
      541 acttcttcag caattgcaac tgtctgtttc tcgttgttcc cgctgcaaat gatgaagtaa
      601 tccgtaatcg agctggtttt agacacattt aaaacaacaa agttataggc ttttttatta
      661 tcgcaagctt ttaaaatagt ttcaatattt ttttgaactt ctatcattta ccctccttta
      721 aataattcag tgtatttact gattccatat caagaaccaa attcctgttt ttaacgtatt
      781 caattgtgtt ttccaaagat tttattattg cttcatcaag tctagtatat gctttatttc
      841 taatttcttc aacaccttca taattcctgt ttggctcaat tttatctgca atatatatta
      901 ttttttccaa aagagacata tttactcttc ccgttgtatg aaacattatt gcattgagta
      961 tatcattgtc ttttactcca tactttaccc cggcaagctc tgcacttttt tttgcatgtt
     1021 tcaaattgtc gctgcatatt ctttttccgc agtcatgcaa taatcctgca attattgcct
     1081 ttgaaacatc acaaccataa accgaagcaa gtttttcagc ttcttccatt gtaccgatgc
     1141 tgtggcggta cagcttttca cctaattcaa tttttaattc ttttttcatt tcttcaatat
     1201 gcattttact ctccgctgtc taaataatgt gatatccttc acttttcttg cccgttttta
     1261 tacaagctgt tcttaattat atattgctcc acagaatcag gtaacagata ttttatggaa
     1321 acacccatct tgatcctatt tcttatatct gaagaagata tttgaagcat ggggacctgt
     1381 aatatttcta tatttccgcc aaacattctt tttatttcat ttatctttgt gtttacttcc
     1441 tcaatactaa tccccggtct tgtagctgca ataaacctgc aaagctttag aacttcttca
     1501 ggctggtgcc agcttggaag atttaaaaca gcatcagtac cggttataaa ataaaattct
     1561 gtgtttttat acagcttgtg taattccctt aaagtactca aggtataact tttgctcggg
     1621 tttttaattt caatatccga tacactgaat ttgtcattag tcagcactgc catattaacc
     1681 attaacaatc tgtgaaatga ttcagcatta ggataatctt tatgcggcgg gtcacctgtc
     1741 ggaataaaaa taatttcatc taatttatat atatttaatg cttcatttgc tgcaactaag
     1801 tgtcccatat gtataggatc aaaagagccg cccattattc caactttttt tttcattgct
     1861 ctcccctttt tctaataaac ataataacag ctgcaaataa aataatactc aatgtaaata
     1921 tatcttcata gggtcttatg ttgttaaaaa gcaatttgct tattgaaaat cctaatattc
     1981 ccgaaaaaat ataaggaaaa tataatttta tcttgctttg tttaacatct tttattcttt
     2041 gaagcatact ttctcttccc tgaaagtaac cattcataaa accgttgtaa gaaataaaaa
     2101 gtataaagac tgacaggata attacaggta taaacgccaa ataaaaacta ccgtctaaac
     2161 aaagcataac agacatggat atgaaaagcg gtgccaaaat tgcctgagtg aaagaatccc
     2221 ccaatcctgc cgatattccc atcatacctt tttttatgta ggaaatatcc tcaaaacttt
     2281 gattgtttaa cattctttct tcctccaatg aaattatata tccatgtatt ggagtaccca
     2341 tattaggctc agtattaaaa aattcagtgt gattgtgtat tgcctctgct atttcatgtt
     2401 catctgcata taattttcta attatatttt tcatactgtg ggcaaacgct aatcccataa
     2461 gtctttcata gctgtagcat gaatgagaat aattcatcca tataaaccat gaataaacca
     2521 agtctcctct ttttaattta ccgttaaaac tattcctgac aatgctcact ttcttctctg
     2581 acaaatataa aaggctcaat actataagaa taaacagtat atatgctctc atatctaata
     2641 ctaaggctgc caaaaaaaca gaaataaaaa ttgcataatt atatttaatc ttaattttgt
     2701 ataaaacatt tgaaacggaa aaaataacca gtattatccc tatataataa agcaatacgc
     2761 taatactgtt aaaatgtatt aatttattga ataaaaacat taaacagaat gccgttataa
     2821 taaccgccaa actcatcata aataacagca gctgcggaaa aagtccgttg tacaaactta
     2881 tgtcgggagc tttgttctct ttatatttct tctcgtaaat atgtacaaaa atattattta
     2941 ttttcaacct gtatttccat atcaatatcc ctaaaaaccc gaagggaaaa gctgctgctg
     3001 ctgcttcaag aggattaagc ttaaatatta ttgaagctgc tgccgccaat atagcagtta
     3061 gacattgatc tcctttcaat gaacccccgg tagaaataaa gtcaataaaa ataatattaa
     3121 ctactgcgcc tgccaacatg cctgttttta tatcagcaag aattaaacct gcagccattc
     3181 ctgccattat aggcctgtat attgtataat aaccgatacc tacggaaaat gcagtactgc
     3241 ttgcccaaaa ataaaaaatg ctaagtaaac aataatcaga aaaagtcatg tcaattacct
     3301 tttattatga tagccttatc ctttggaagg attcttattt caacatatat attgtcattt
     3361 gctatgtcat ttaatatatt taattcatcg tcacttaaat gaatggaatt ataatatctt
     3421 tttcttccct gggctgctcc cactccacct atgtttatac tgcttatttt tgtttttttt
     3481 attaattttt ttataccttc aatactcctg agaagtacca gtatcttgtt ttgattgtca
     3541 tcgcttaaat aagcagccgt ttcatcgatt gtcttcacat gaactataac accttcaggc
     3601 cctgaactct ttatgagttc actcataaat tcatcatttg catattcatc atccgcaatt
     3661 acaacctctt ttgctctaag gtaaggtatc caagatacaa caacctgtcc gtgcaacaat
     3721 ctatcatcaa ttctaacgaa tacatttttc attttctacc tcagtctgat aagaactttt
     3781 cattgacaaa cactatgctc ttcttcccgt ttttaattat tttttctgca atttcctcta
     3841 agctgtatgt atcgcgagca acagcaatat cgataaacaa ggggaagttt atacctgtta
     3901 tatgataaaa atttctgttt ttcattgcac gatttactgc attgtaagga cttccgccgt
     3961 acatatccgt aaatacgata agttcatcat cagatatatt tttaatattt tcttcaacct
     4021 tttttaacaa tgtttcaaaa ctgtcaccat ggttaaatga tatacttttg gcattttcta
     4081 cttctcctat aatcatttca gagcttttca ataattcttt accaaactct ccatgtgtca
     4141 caagtaatat atttatcata ctttcatctc cttgttatat aataccataa ttatacataa
     4201 ttttcatcaa tattttagat tgctttttcc gcccgtcttt tattgtaaca tagtaaagat
     4261 attctcctca accctgtttc ctttgactaa tagtttatat gaatttatta aaatagtaaa
     4321 gaattcttta tttttaattc actaatattt ttttcaaacg tatattaata ctgagaaatt
     4381 tatttaatgg aggatagtat gaataattat agaaataata tgccccacta cgactcactg
     4441 ccccataact ataatccaat gggcataaat gagcacaaaa attctaatag aatgcatctt
     4501 gcaaacacaa tgcccagaat gccggggatg cctgacgata tgctccaaag gccattcatt
     4561 ccggaaacgg aaatgccttt agaaattgac ggagatatgc ctgctctgcc gaggtttccg
     4621 caacaaggga ttcctagttt aataccgcca tggttgacac aacccgaaat acccatgatg
     4681 cccggtatgc agccgactcc cggaatgccc ggaatgccgc aaatgcccgg tatgcagccg
     4741 actcccggaa tgcccgggat gccgcaaatg cccggtatgc aaccgactcc cggaatgccc
     4801 gggatgccgc aaatgcccgg tatgcagccc actccaggta tgcccgggat gccgcaaatg
     4861 cccggaatgc ctcagatgcc cggaatgcca aacggaatgc ctcagatgcc cggaatgccg
     4921 cctgatgatg gcaatatgta tcctatgatg cccgggatgc caaacggaat gccccctatt
     4981 aactgcgagc aattaagaga actgatgaga cgtatgaact gcccgatgga cggtggagga
     5041 ggagaaaatg gaactgtccc gcctatgaat gaggaaatgt aagaaaaaca agtgtaatat
     5101 ttaatggcag aaatgctgtt aaatattact tttatttaaa gtttttaaag tgtacataca
     5161 gtttttttaa tatcttgcat ataatgtata actttggggg tgattttgat acaagagcta
     5221 ctacaaatct tattaccgct atcaatattt atttttggtt acataactaa cggaagctca
     5281 tttccacatc ctttaaccaa ggaagaagaa gcatgcctta tagaaagagc aaataaaggc
     5341 gatatagaag caagaaatat tcttatagaa agaaatcttc gtcttgtggc acatgttata
     5401 aaaaaatata agacagtagg aatagaccaa gaggatttaa tatccatagg aacaatcggt
     5461 ctaattaaag ctgtttcaac ttttgatgaa aaaaaaggaa taagacttgc cacctatgct
     5521 gccaaatgca tcgacaatga ggtacttatg actataagaa gtgaaaaaaa actaaaaaaa
     5581 gaagtatctc ttcaaggacc gttaggaaca gacaaggaag gcaataatat ttatctcatc
     5641 gatataataa aatatgaagg agaagaaata gtaggcagat tggataaaga agaaaaaata
     5701 aaagccctgg atgaaatatt ggataaaaca ttacaaaaaa gagaaagatt tattattgaa
     5761 atgcgttacg gaataagaaa caaagagcat aaaactcaga gggaaattgc caaacttctt
     5821 ggaatatcac gctcctatgt atcaagaatc gaaaagaagg ctctaaaaaa actccatgac
     5881 gccttggtga acaacgttgg aaattgatga caaggggacg ggggtgctgt catcattcag
     5941 atgacagtgc ccccgttccc ttgtcattta tttaatttta ttcttccagt ttttttctca
     6001 aaataaagta tggctctaca tttttttcaa tatacatttt tactgtttgc tgcggatggg
     6061 gtgctgaatc tattttatta ttattttcat ctgttaagtt ttctgctttt aattcaaagt
     6121 gttttccttt aggaccaaag cattccaaaa tatcgccttc tgaaaattta tttctctgct
     6181 ccactgttgc aatctttgat gactcgtcat acgaccttat tatcccaacc acatcatatg
     6241 tccttatata gcttgaatgg gtatacaact gtccatcctt gtagggatta ccgaagaaaa
     6301 atccatagga aaaatccctg tgcgatgttt tttcaagttc ttccttccaa tatccggtat
     6361 ttctttcact ttccggatat ttcagctcat ccaatgcatt tctgtaagag cggacggtta
     6421 aagcggtata gtattcactt ttgtttctac cttcaatttt taacgctttt attcccagct
     6481 ccttgaattt ctgcaaatgc tctatcatgc ataaatcctt ggaattcata ataaaagtac
     6541 cttcaccggt ttcttctatg ggataatact ccccgggtct tttctcttca accaagttgt
     6601 atttccatct gcaggattgg gcgcagtcac ccttatttga aaacctgccg gtcataaagc
     6661 ttgataagag gcatctgcct gaataggata tgcacatggc tccatgtaca aatgtctcaa
     6721 attctaaatc ttccggtgtg ttttttataa tagtttcaat ttcctcaaat gtcagctccc
     6781 tggctagcac aattctgttt actccctgtt tataccagaa gtttgctgtg tgatagtttg
     6841 ttgtatttgc ctgggtgctt aaatgaattt tcatatccgg aattgtttgc tttacaattt
     6901 ccacaactcc cgggtcagct acaatcagtg catctgtacc gattttatgc aaatatttca
     6961 aatatccttc tatacccttt aaatcatcat tatgaggaat tatattaacg gtaatatttg
     7021 cttttcttcc atatttatga caatactcca ctccctgttt taaatcgtct ttagtgaagt
     7081 ttttgctccc tgctctcatt cccattgatt ttcccgcaaa ataaacttca tcggctccgt
     7141 attgtacggc cattattaac ttgtctaaat ttccggcagg tgctaataat tttggcaaca
     7201 ttctttctcc tctttctcta tctggttatt tcaattaaag cgacattctt taaaaaacaa
     7261 agttttcggt gtgttccatc cctatttaat cagcgtaact gccaatccat ccccaagggg
     7321 aagtaccgaa gttacaaatc tctcgtcatg agatatatat tctaaataat ttcttaaatt
     7381 tcttactaat gttttctttc ttcttactac caattcatcc gatgcaatca ttccttgata
     7441 taatacattg tccgaaagaa taatccctcc gggcttcatc atttttaagc atttgtcaaa
     7501 aaataccctg taatagcttt tggctgcatc tataaaagcc atatcaaaag tcccttctac
     7561 ttggtccaat atatcaacag catctccaaa ataaacctct attttttttt caagtccaaa
     7621 atcagtaata ttttttaatg cttcattata aaatttttca cttctctcca atgtgacaac
     7681 cttaccctca tctttcataa ccttgctcat aaatattgaa gaataaccta cattggtacc
     7741 tatttcaatt atactttttg catttaaaag atttataact aatttaataa attggcctac
     7801 ctccttatgg atgataggca gacttctttt ttcgcaataa tctctgaatt tatttaattt
     7861 ttcgctatct tcaataataa ggctgtttat atattcattt atataagatg agttaatatt
     7921 attcaaagta tacctcctca attcaaatac tcttcagccg catctatatg ctcatcatat
     7981 gtgtttgaga aataatgaga gccgtctcct tttgcaacaa agtataaata atcaacatct
     8041 gcaggctcca atacagctat tattgatact ctgccgggtg aatttatggg agttggaggc
     8101 agtccttcca caatataagt attgtatggt gaatcaattt caatatcctc aagggtaagt
     8161 ctttccttcc actctccgtt tgcatagtta acggtcgcac aggattgaag cttcatgtta
     8221 atactaagac ggtttaaaaa aacttttgca acttcatctc tttcttcatc caaaagagct
     8281 tccttttcaa ctatgcttgc taaatttata acctcttcaa atgtaaggtt tgttttatct
     8341 atccttggtt taatttcatc aatataaaat ttatcaaatt gttttaaaag aactctaata
     8401 atatcttctt ccgttgaatc aacataaatg ttgtaagtat caggcattag gtatccctgc
     8461 aaatttgtaa cagatgaatc ttttaagaat tcaaaatctt gattgaaaat ttccggagat
     8521 tccatcaatt caataaatct gtcataattt aaagcggtct gctccgacaa ggactgagcc
     8581 atatcttcat tagtcaaacc ttctatcact gtgatattgt aagtattgcc tgatgagcct
     8641 ccttttataa gggattccaa aagttcatca gcattcatgc tttttgaaag aatataggtt
     8701 ccggccttta atttagcatc tgaccctgtc ttttcagcat aatacttaaa tacgcgtata
     8761 tttgaaatta aattattttc aaacaaaatt tcagcaatgt cattagttgt gcttcctgcg
     8821 ggaatttcaa caacaatcgg agaatcatcc cccaaatcaa cagcttctaa attttcttca
     8881 atataattat ttaaataaag atacgccgct gctattattg acaaaacaca cagtagtgca
     8941 attatctttt tcataattcc tccagtagat aatttctaaa tccattaaat tatacaacct
     9001 tttactaata attacaataa tttaaaaaat taatagtata ataattattt gactggttaa
     9061 tataataatg cttatttctt ttgattaaga gagggttgat actaatggac agattgaaca
     9121 aaattagcac tcccgattta tccaaagttg gtaatttaga tgtgattaaa cttagaaggg
     9181 atggtcaata caatttaacc agtgctatca acggtagaat aaccccaaat ctaaatttga
     9241 cttcaaaaag gtcaaatcaa agaatggaag ataaaagtat tgtaaacaac aaaccatccc
     9301 agttcagacc aatcttagga gtaaactttc acaacatcaa aagataataa gcattaataa
     9361 ccggtgacct aaaagctcac cggttgtttt tttaccccat tttttcaact aaaaaataaa
     9421 tagggactta ttgtatgtct tcctgaactt agtgaaggat tttatcgaca tattatggat
     9481 ttggtaaagg tgtcagttca gataaatccg taaaacaata ccaccatgca agacagttca
     9541 gttctaaact ttcgggatcc atgcctcttt caattgcctc atcgagggtg cttgcaacag
     9601 gaacaattac cgggtccccc ggcatccagt ttgcaggggt gcttacacca tatgttgttg
     9661 acacttgcag tgcatcaatt aatcttaaca attcatacat gtttctgccg ttagttgcag
     9721 gatagatgag aattgacctt attctttgat ttgggtctat aataaaaaca tctctaacac
     9781 tttgctcata gattctgtca ggactaacca tgccgtaaag cgctgctata cttgcatccc
     9841 tgtcggcaat aagaggaaag ggcataacta tacctgtcat ttggaatata tcgtagagcc
     9901 agccaaggtg tgcaggattt gaatcaatgc ttattcctaa gagctgaaca tttctttttt
     9961 caaattcagg ttgaagttga gcaaatgcaa taaattctga tgtgcaaaca ggagtgaaat
    10021 ctcccggatg gctgaaaaac aaaacccact tccctctata ttgagacagg gttattggtc
    10081 cctctgtagt aacggctgta aagtcaggtg caatccttcc gattgtcagg catacccttg
    10141 tacgttcggc ttctatagtt tcattattga tcataatttg tttgttacct cccatatgta
    10201 ttttatttat tatatgaaag gacaaatgat atagttcgaa tctattacac tataaatggt
    10261 tatgagattc ttcactgcgt tcagtatttc ctggtgcctg gcaccannnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnncctg gcaccaatgt tcttaacaaa aatagggacg
    10441 ttaagtccct atttattttg tgtccctaaa tttatacttc caaatcaact tctactataa
    10501 tgggaagtat cattggagtt ctttttattt tttcataaat aaagctgcct aaggaatcct
    10561 ttattaccat tttaatattg ttccattctt taacatttgt atcttcacac ttttccaatg
    10621 cttttcttac cactgttttt gattcttcta ttaaatctat attctcgcgt acatatatga
    10681 atcctctcga tactatttca ggaccggcta aaatttttcc cgtacttcta tcggtagata
    10741 gtacaactat tatcaaacca ttttctgaaa gaagtcttct gtctcgaagg actatattcc
    10801 cgacatcccc cactcctaag ccgtctacaa gaatatttcc ggatgtaaca gtgttgtttt
    10861 taactattga cttggaagta agctcaatta cgtcaccatt gctgcctatt acagtattat
    10921 taatgggaat gcctatttca ttcgccagct ttgcatgctg caccaaatgt ctgtattcgc
    10981 cgtgaactgg tatgaaaaac tttggtttta acagagtaag cataagcttc aattcatctc
    11041 ttctggcgtg gcctgataca tgcacttgtg tcagtgcatc ataaagcacc tttgcgcctt
    11101 tttcatagag catattgatt actcttgaaa ctgttatttc atttccgggt attggattgg
    11161 ctgaaattat aaccaagtct ccttctttta tttgaatttt tttatggtca ttgttagcca
    11221 ttcttgtcaa ggcagacata ggctctccct ggcttcctgt agtcaatata acaatctcat
    11281 catccttaag gctgtttagc tcgtttacat ttattattgt tccctgtgga gcattcagat
    11341 atcctaattc cagagaaact ttaattgcat ttattatgct tctacctgat attgcaacct
    11401 ttcttttgtt aacgaccgct gcgtcgataa cctgctgcag cctatgaata tttgaagcga
    11461 aagacgcaac aattattcgg gaatcacagt ttttaaatat atccataaat ttctgaccaa
    11521 ttgttttctc tgattccgtg aagccaggcc tttcaacatt agtgctgtca gaaaaggcaa
    11581 gaagaacgcc ttttcttcca atatccgcaa acttacctaa atcgataaca tcttcattga
    11641 tgggtgtata atcaatttta aaatctcccg tatggactat tgtaccgata ggtgtatcta
    11701 tggcaaagcc cactgaatca ggtatactgt gattaactct tataggcgta attttaaaac
    11761 atcctaattt taatgtatcc ccagcattaa tgggatttaa ggatgcatct tctattttat
    11821 gttctaaaag cttattttca acaagaccta aggtaagttt tgttccgtaa atcggtatat
    11881 ttagcttttt taaaaagtaa ggtattgagc cgatatggtc ttcatgacca tgagttaata
    11941 taataccctt tactttttct ttgttcttta ttaaataagt aatatccggt atcactatgt
    12001 caataccaag catttcatct cccggaaaag ccattccgct gtctattacg ataatatcat
    12061 tatgatattc aataacggtt atgttttttc cgatttcacc gagtcccccc aatggtatta
    12121 cttttaattt atgtttcttt tctgccatct ttattctcct tatctctgca ttgtgcacag
    12181 attccggtga atttgaccac atgatttaat atttcaaaat tgtattgttt ttcgattatt
    12241 tcttccaatg tgttcataag gtcttcttta acctctatga ttttgccgca ttgtttgcac
    12301 aataaatgat ggtgctgatg aatttcatta ctgttttgtg ataatatttc atatctggaa
    12361 aacccgtcat cgaagttgtg cttatataca agtcctatat cctcaaacaa gagaagggtt
    12421 ctgtaaatgg ttgctatgcc aatattgggg tacctgtttt ttattaggtc gtaaatttct
    12481 tccgtagata aatgcttatc tttgttatca gaaagaactt catataaaat ttttcgttga
    12541 acagttgttt tgtaaccatt tttatcaagc aaagttctta aataatctgc catttatcta
    12601 ccccccagga cacagcttaa attaaatcat cagctattcc ttcatatatt tcctgcactt
    12661 ccttaatttc ttcctcgtct tcaactttaa ccagcactat acttccgtct tccatttctt
    12721 ccacataata aaccatacct tcatccgagt ttattttatg aagtactgca tacaaaatat
    12781 catcaatgtg aaaagtggct tctacgacta acttttctag ctttccttcg tcgtttatca
    12841 gttcaataat attatccata atatctcctt tgattatttt gtacgctgca tataacttga
    12901 cagaatttgt accgcagcta atttgtcgat aacttctttc cttttttttc ttttaacatc
    12961 agcttctatt aacattcttt cggacatggt tgtggtaaac ctttcatctt caaactcaac
    13021 gggtaaatct atatgctttt tcatcttttc caaaaattca agcacctttt ctccctgtat
    13081 tccaattgta ccgtctaacc tttttgggat gcctgccact attttagtaa cctgatattc
    13141 atcgatgagc tttttaagtt cgtttaaatc atttttaata ttcgttcttt ttattgtagt
    13201 tactccttgt gcagtaatca tcaataaatc actgacagct actccgattg ttttatctcc
    13261 cacatccaat cccatcaatc tgtccataga aacctcacac aattttgata taaaaacctg
    13321 cgcattcacc ggcgcaataa ctgcataaaa cacacttttc catatctacc gcacatttgc
    13381 cgtcaactat tgcaagtgca ttttgtttgc atttttttac gcattttccg catccagcac
    13441 accactcttc tattacgaga gtgcgatttt gattttttat tttgtttttt atattaatat
    13501 ttaactcatt attttccaaa taagagatat ttgcatctat ttcttcaaca gtgctcatac
    13561 ctattgctat ggaatccaca taatttaaac tgtcaatata cttaaatgct ttttctgctt
    13621 cctttattaa atggcctccg ccatatattt tcattgccat aataaactta ttattttcct
    13681 ttgcctcctt caaggcttca gtcatatctt caaaagttcc gtcttgaatt ccgatacctt
    13741 tataattgat caaaggaaaa ataatttcta tttccttaaa tttaagtgaa tcttttactg
    13801 cctccaccct gtgagttgaa attccaaagt acttgattat tccctgctcc ttatatttca
    13861 tgaaacgctc aactgcctta taatgaccca aaaaattatt cccgttatcc tgttcatgaa
    13921 gcatgaaaat atccaaataa tcagtgttca tttctttcaa tgctttattg atactatatt
    13981 ccgcagtatt tttgtcatat gcataacttt ttgaagctat aactagttta tccctgtctt
    14041 tatgttttat gaattcttta agtatattat agttatcata aagctgggct gtgtcaaaaa
    14101 aatttattcc tttttcgtat gcataattaa gcagccttac tttattttca acagtatcga
    14161 gattttgaag actgcttaaa gatagtgttc cgtaacacag ccttgataca ttttcttttt
    14221 caaataaatt tatgtatttc atatttatat ttttatagga agaaacttga gggcatacaa
    14281 aagcagtata tttatatact gctcaagata taagttagta cagggacatt caaagtgtgt
    14341 cccccctcct ctaaatgtcg attacattta agtaaacact taaaatttct tccaacagtt
    14401 cgtctctttc tatttttcgt attaaacttc ttgcattatt atgatttgta atataagtag
    14461 ggtcccccga taatatatat ccgataagct ggttgttagg attgtatccc ttttctttaa
    14521 gcgacttgta gacctgtgaa attatttccc gagcttcatt caatgaaccc atactgtaat
    14581 caaattttgc cgtattattt acattactat ccataaaaac cacctcttta cacctactta
    14641 tattctacta tagtttttaa ataaaatcaa ttactatatt atgatataaa acaacatgaa
    14701 attcttcgtg ctaatggtac tcaaaatgat gacagtaccc ccgtcccctt gtcatataga
    14761 ggctatttgt tttcttaccg cattaattgc ttcgtctatt ttctcggggt ttttccctcc
    14821 tgcttgcgca aagtcaggtc ttccgcctcc gcctccgcct gcaatcacag ctgcctcttt
    14881 aaccatattt cctgcatgta ttccgccttt tgccaaatcc tttgttacag ccgatacaaa
    14941 taagacttta tcccctaaat ctgaagctaa tattatagca cagctctcat tcttatctct
    15001 aattttatcg gcaatttctc ttaaggtatt tgcatcctta tctttaaatt ttatggcaaa
    15061 aagtttaata ccgttaatct cttcatattt gtcaagaatc tcatttatat tgctctttaa
    15121 tatttcattg tttaaccttt gaatttcctt atctttttct ttagcttcct ttttcagaag
    15181 atttgctttg cttaacaatt catccttggt tgttttcatg gatatcgaca attcatcgat
    15241 taaattatcc aattcctgca tataatcaat ggctgctctt cccgtaatag cctctatcct
    15301 tcttattccc gaagctatgc ttccttcgga gataatttta aacataccaa ttttagctgt
    15361 ttggtcaatg tgagttccac cgcataactc cttgctgaaa tctcccacgc ttacaactct
    15421 taccatctct tcatactttt catcaaacaa agctacagca ccggactttt tagcttcttc
    15481 catactgagg atatccgttt taaccgtgta gtctgatagt attgcttcat ttactctttt
    15541 ttcgatttct tttaatgttt ttttatcaag agcttcaaaa tgtgtgtagt caaatcgaag
    15601 tctatcttca gaaacatatg agcctgcttg atttacatgg tctcccagta tttccctcaa
    15661 aaccttgtgc aatatatgtg tgcaggtgtg attctttgca gtatttttcc tttggtcatc
    15721 atttactttt gcagttattg tatctccgac ggaaatactc cctttaacca gattggtgtc
    15781 atgcagataa atcccattat tgaatttatg agtatcggtt acctttgcca gcatttcatc
    15841 tgcagtgaat tcacccgaat caccggtctg tccgcctccc tcggcataaa atggagtttt
    15901 atcaagcact ataattccgg actgtccttc atttattgta tttacctgct cagagcccac
    15961 tacaatggca ataacttcgg atatatcttc taatttattg taacctgtaa atatggtttt
    16021 atccgtatcc aaggtaaaat cattgtcctt ccaacctgaa gcgccggtag ttttccttgc
    16081 ttctcttgcc atctgcctgt gctcttcgaa ttctgcgtta aactgctctt tatttaggct
    16141 aaaaccattt tccttaagta tttcttctgt caattctgaa ggaaaaccat aggtgtcata
    16201 aagtttgaat gctacatttc catccagctc ttttttattt gtttctttta gtttttgaat
    16261 ttctttcatt aaaagttcaa gaccttggtc aatggttatt ttaaatcttt cttcttcaag
    16321 acttaaaacc tttaatatgt atttttcttt ttctcctaat tccggataaa cttctttcca
    16381 attatctatt acaacctttg cagtcttagt taaaaattcg ccatttatcc ctaatgattt
    16441 gcccttgcgg attgctcttc ttattaatcg tcttaataca taacctcttc cttcatttga
    16501 aggtattact ccatcgccta tcatgaaggt cgctgctctt gagtggtcgc agattacttt
    16561 cattgcaacg tcgctttcta gattttgacc ataaggtatt ttgcagattt ccgaaacttt
    16621 attgactata aaaactaggg gctccacatc aaacaccgtg ggtttgtctt gaagcacgca
    16681 agctaccctt tcaagtccca ttcctgtatc tatattcgga ttaggcaggg gatgatatac
    16741 accttcttcg tccttgtcaa attgtgtaaa tacaagattc cagatttcta tgaacctgtt
    16801 gcagtcacat cccggtttac agtcagttga accgcagcct ctatcttctc ctgtgtcata
    16861 atatatttca gagcagggtc cgctcggtcc tacttctaac tcccaaaaat tatcatcctt
    16921 tccaagcctc actatttttt ccataggtat tcctaccttg gtatgccaaa tattctcagc
    16981 ttcatcatcc tccaaatata ctgtaaccca aagtctatcc ttatcaattt ttaaaacatc
    17041 ggttaataac tcccacgccc atacaattgc ttcattcttg aaataatcac cgaaggaaaa
    17101 gtttccgagc atttcaaaaa aagtcaaatg tctcgctgtt aatcctacat tttcaacatc
    17161 acccgtcctc acacactttt gacaggtcgc cattctctta gcaggcggaa tttcagctcc
    17221 cgtaaaatat ttttttaacg gagccatacc tgctcctatc aataataagc tcttatcatt
    17281 tttaggtatc aaagaaaaac tttgctcggt taagtgtccc ttgctcttaa aaaagttcaa
    17341 gtattcttta cgaatattat taattgaaaa tctatcaatt ttatccactt tatttcctcc
    17401 gtttcatagt ataagcaata tgtactttat tatgaaatta taatgtattc tttccctcat
    17461 tgtcaagaaa catttaggga cggtactttt tgttcttaga taagcgactg atgttcgctc
    17521 aaatgataac aaaaagaacc gtcccccaaa agtcgttcac tgtcttgtgg gatctttagt
    17581 gttgacagtg tatctcatca tcctcaggtt tataattttc caaaccctta attaccttgt
    17641 ttgctttttt tgcataatca taaagggctg attttacagc ttgctctgcc aataccgagc
    17701 aatgcacctt aacaggcggc aaccctccta aggcttctac aacagctttg tttgtcagtt
    17761 caaccgcttc atgaatgctc tttcccttta taagctctgt tgcaatactt gatgaagcaa
    17821 tcgctgagcc gcatccgaat gtttcaaatt ttacatcctc aattatatca tctttaatct
    17881 ttaaatatat tttcattata tccccgcatt tggggttacc tacctgtcca acaccgtccg
    17941 gtttctccat atgacctaca tttcttggat ttctgaaatg gtccattact atttctgaat
    18001 acataattcc ctccgtaaat tagtttttat gcaaatcttc atcgttttta atcggtgaca
    18061 ttgctcttaa tctggtaatt atttttttca ttgtttcaac agtataatct atatcctctt
    18121 ccgttaattc ttcacttaag gtgaaacgga ttgagccgtg ggctgttccg tggtcaagcc
    18181 ccgttgccaa caaaacatgt gaagggtcta atgaacctga agtgcaggcg gaaccgcttg
    18241 aagctgatat tccttccata tccagaagca ataatatgga ttcgccttcc acaaatttat
    18301 atgagaagtt tacattattg ggaagtctct gtgtcggatg cccgttaagc actacatcgg
    18361 gaatattgtt tttaattccg tcaataagtc tgtttcttag aactgtcagc ctttccacat
    18421 gcttatcaaa atccctcgca gcaatttcga cagctttccc gaaacctaca atattctgta
    18481 ctccttcggt accggctctt ctgcctctct cttgaccgcc tccgtgaata aagttatcaa
    18541 ttcttgttcc ttttcttata tacataccgc ctattccctt aggtccgcct attttatgtg
    18601 cagacattga catcaaatct atatgaagct tattgacatc tattctgata cctcccaaag
    18661 cttgaacagc atctacgtga aataatatac catggtcctt agcaattttt cctatttctt
    18721 cgattggctg tatggtacct atttcattat ttgcgaacat tactgaaata agcattgttt
    18781 caggtcttat tgattctttc agctgttcta tatcaataag cccgtatctg tcaacatcca
    18841 gataagttgt ttctattccg aatttttcta agtattcaca agtatgaaga attgcatgat
    18901 gctcaatttt tgttgtgatt atatggttct ttcctttgtt cagatttgca aatgcaactc
    18961 cctttaaagc ccagttatca gactccgtac cgcttgcagt aaaatatatt tctttttctt
    19021 ctgcattcaa aaagtctgcc aaagtttttc ttgccttttc aatggcttct tttgattttc
    19081 ttcctaactc atataattga cttgatggat ttccgtacaa aatatcaaaa tattgtctca
    19141 tttcatcaat tacttctttt ttcacccttg ttgttgctgc attgtcaaga taaagtttcc
    19201 tgctcatttc ttccctccgt taaaatgttt ttttgctctt cgtacatatc agctaaggtt
    19261 atattatcaa taacatcgtt aatactcttt tctatcttct caaatataac tcttgtagca
    19321 caaattgatt ctctggagca tacttctttg cctaaacaat cagaagtttt tatttcaccc
    19381 tcaagtgttc tgagtatcat tccaacagtt atttcctcag gtttttttgc tagaagatat
    19441 cctccttgag gtcctctgac cgtatctatg taaccgcttt tttttaattt tgcaaacagc
    19501 tgctctaagt aattctctga tataccatac ttttctgata ttttctttag agaaacaggt
    19561 cctaatccat aattttttga aagttcaaat gccgccttaa gtccatatct gccctttgtg
    19621 gataaaatca tttttactct cctttcatgg aatccctact gttttagtcg ggtataattc
    19681 attatagaat accctagtaa ttttgtcaac attaaaaaaa ataaaaataa taaaaatatc
    19741 agcggatatt tttattattt tttgcttata aattttatgg taataattta tatacaaagg
    19801 aaccattttt gtttatttga gtataaacat tgcttacggg atatctaccc ctgcattcat
    19861 ttaaaataat atccaatgtt tttctatcgt ttgtctttat gcataaatcg cactcattct
    19921 ttatgctgaa aggggtttga atcaaatgta tattcttaac tccaaatcct tgcagcctgt
    19981 tgaatgcggc aacagcataa ttcccgtgca aaaaggtaat aatatagact tccataggat
    20041 acctcctcgc ctctatttta ttaatattag caaagaaggt tacaaaaagg ggacatttct
    20101 ttgtccccct tcctctaatt tatatattta tgttcctcat cattttcata tatatttagt
    20161 tcatctttca aaaattctaa atttgattca tagtcttctt cagaaatttg attgctgata
    20221 agtaatgcgt ccaacttgtt tttctgattt tcatataaaa atatttcttt gcaaggccac
    20281 actacatttt tctttttatc accctgtgat ttcataatcg aaatatttat ttcattttcc
    20341 ggcaagtcgg atatggtacc catcaaataa aactcattta ctcgaatttg attgttttcg
    20401 aataaagctc tgattttttt tataaacaaa taatgctcct tcttaatttc ataagaaata
    20461 tcagcattta gcatgccttc ttccaaatcc gtgttgtaaa aatcaacata tatatcgtat
    20521 acttgatcaa cattcaaatt gtcaaaattg agcttagagc ttataatatt ttctatattc
    20581 gggtcggtaa tttttccaaa aattttgtac atgctgctcc tccttactgc ctcaaatatt
    20641 ccaagaattt cttaattctg ctttcgtagc ctgattcctt aggctcataa aatttagtat
    20701 ttattgtttc aggcatatat tgttgtttta cataaaattt atcataatca tggggatatt
    20761 tatattctat tcccctgttt agcttttcag cacctttgta gtgtttatct tttaaatgag
    20821 caggtacttc atgagattca ttttcaactg cattcattgc tttgtcgatt gcaattattg
    20881 aggcattgct ctttggacta catgcaatat atattgcggc ctgggcaaga attatccgtc
    20941 cttcgggaaa gcctactatt tcaactgcct taaatgcatt cacagcaact tccaaagctc
    21001 tggggtctgc atttccaacg tcttccgaag cgcagataat tatccttctt gcaatgaact
    21061 tcgggtcttc tccggccttt atcatttttg caagccaata aactgccgca tccgggtcag
    21121 agcccctcat gcttttaata aatgcggata ttgtatcata atggttgtct ccatctttgt
    21181 cgtaatcaat tttctttctt tgaatacatt cagatgcaat atcaatattt attttaatta
    21241 ttccgtcaat ttcttcggtt gtttccacac caagctctaa tgcgttaaga gcgcttctta
    21301 tgtctccgtt tgcgtattct gcaatatgag ctaaggcgtc ttcatcgatt tccacttttc
    21361 tgtttccata tcctcttttg tccaccaatg attttctaac cacattcttg atatcctcta
    21421 tgtcaattct tttgaattca aataccaaag agcgtgaaat caaagcggaa tttacttcaa
    21481 aatacggatt ttcagtggtt gctccaatca atattattgt accattttc
//
LOCUS       DAOSHP010000024        21218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2015_length_21218_cov_9.164154, whole genome shotgun sequence.
ACCESSION   DAOSHP010000024 DAOSHP010000000
VERSION     DAOSHP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2015_length_21218_cov_9.164154"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLB33_03910"
     CDS             complement(<1..301)
                     /locus_tag="PLB33_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose dehydrogenase"
                     /protein_id="HOG62700.1"
                     /translation="MKKVNVSLRPIVSNINLPTVIKTAILPGDTMESIFVATQVGEIF
                     YIRNRIARLFLDIRQRIIELGTNGGYDERGLLGLAFHPNFYYNGLFYLHYSKAGTQ"
     gene            complement(411..839)
                     /locus_tag="PLB33_03915"
     CDS             complement(411..839)
                     /locus_tag="PLB33_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG62701.1"
                     /translation="MLNGRTYINRKVNKLESSGNTGFLEVRVFSEEGNPIEGARVSVS
                     LYELRGIYRESAIENEIVSHYTDENGKIPIIELPVIHELGEANTDEYHMRVDAPGYYS
                     VIVMNIEIFPNTTTSFNVVLTPVMEGESYVRFIIIPEKHW"
     gene            982..1509
                     /locus_tag="PLB33_03920"
     CDS             982..1509
                     /locus_tag="PLB33_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG62702.1"
                     /translation="MVAEELLILNLKRGREEAYRQLIEKYGNRLYRTCYLILNDREEA
                     EDVVQETFIKVFKKIDTFKGQSGLYTWIYAIALNLSRDRIRMKQDMLELKDEWIGSYD
                     VESLVEINIDRELLKKEIFEMNSLYREVLVLFYFEELSIKEISNLLNEKEGTIKSKLS
                     RGRNILKESLLKGGR"
     gene            1509..1820
                     /locus_tag="PLB33_03925"
     CDS             1509..1820
                     /locus_tag="PLB33_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62703.1"
                     /translation="MDKKDKLGIEIKSIMEEETLDLTLSQKAFDNIIQHRKKSLREKI
                     QEFINKEIEIPVAPAIIGFAALLAVTLIPMDIFKSQNERIIDMGGSYVIIREGFEVSK
                     K"
     gene            1817..3988
                     /locus_tag="PLB33_03930"
     CDS             1817..3988
                     /locus_tag="PLB33_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62704.1"
                     /translation="MKIKIKIKTLIFAVFILTLTFIWAIPTATLMIAGYLETKDSEKA
                     SLFYEKYASYPTTPSIESKYLHANSLIRSFNKFTIFLTGWGGGENTSPEDIEKAKQIL
                     METLAEKPVKNREKDYYIKSYKMLLDILIASGDAETLQEWIAFGQKGTDERLVYTADV
                     YDGFLHHVNGEGEAAKSIIEKYDETELKDTIFYVLKAEIALFDGKYEESEKIYDNIYK
                     NNWRELGKSFGSGGYYRNDWFDRFMEDFKGDNVITGTVTCEGKPMPFVEIYVQEAKEG
                     FSFYASGENYVAITDVNGEFKTLGLKDGLYSVGIGVDGSVLANKAFQGSTMQYVELNG
                     GDGEFHLSFRNTLKIYSPEPGEKLSGEEFTVSWEEIEDAAYYKVEPVVFYEPFGERGI
                     STRIYAKDKNGEYKFTENQAVFNINMIRNNPYITVSDSDNGYLGAQAVLGIFIPGVEY
                     PIVVNAYDENNNLITGSLPLRSYYEHIPSITAEGSLNEGENFIYRKNYPAAIEYYENI
                     LKENPDDIDALRYLTRIYGIGWKEGEQNFERAFTLGQRYTEISGNRELLTNTLGGMNI
                     SEIKENRENVYLTLKEAMKEPDFDNYYFLSRYYIAVENYEGAREALQMAENVTDNLIF
                     LNMYFGDYKEAVESIKSERFYRSRLSSNKVKDAIEALDESPPRGDELKVFNDFLLKLI
                     NGIPYGENRNIYNKTVKQISNSNLKTIIDEIYLDRNWDLEY"
     gene            4100..4807
                     /locus_tag="PLB33_03935"
     CDS             4100..4807
                     /locus_tag="PLB33_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HOG62705.1"
                     /translation="MKKFIIEDDFWELFPDAKFGVVICHNIDNKIKEVDKYLDMLRDS
                     EDEALKYLDNPEFIQNDVIKVWREAFQKFKTKKGARCSIEALLKRVKNGNHIGTINPL
                     VDIYNSISLRYALPCGGEDIDAFAGDNRLTKAVGDESFITYGSDKSEPPYEGEIVYKD
                     DQGAICRCWNWRESVRTMLTESTKNAFLVIESVDEKRTEELKNAINDLAKLVQDNLGG
                     TIKTSILDINNKLIDLE"
     misc_binding    4947..5156
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5354..5494
                     /locus_tag="PLB33_03940"
     CDS             5354..5494
                     /locus_tag="PLB33_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62706.1"
                     /translation="MEGSKLFWSSGTWIFFLVQALVVALAVYLYWYLGKKEKEACEEK
                     KE"
     gene            5524..7131
                     /locus_tag="PLB33_03945"
     CDS             5524..7131
                     /locus_tag="PLB33_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOG62707.1"
                     /translation="MKLNVIIPFVSYLLIMLGIGMYAMRRIKTYDDFIIGGRSIGAWV
                     SSFALITSYASGYTYTAAPGMSYTGGWSTMWWSTGDAPGNALSFGLLGRRLRKFSELL
                     GAITLPEYYEKRFKSPGLRLLTSIIIITTVSLHLVAQWQASGILLSVTFGTDYLVGML
                     IGGAVVLAYTIMGGYLATVYTDFMQGVVMFIGTHILFWSAISKVGGFNALNDQLALIN
                     PGLVTPWGPDGSYFGLIAAASPIILIIMGSFGMPHVTVRHLSLKNPDTARKAMLITAI
                     FVALFSFAYYMVGAAGLILLGPGLENPETSGVLLWFEVLPPVGAGILVSAAVAAIMST
                     ADGFLMLIVSTIAHDILNRFLLPNTPQEKRIRIARIITAIIVILSLVAAINPPAGVFT
                     IVMTVFGGMALAFGIPNIFSVYWKRSTKTGAIACVILSLTVYVWLTAAGIELFGMSPF
                     MNGLIVAILAFIIGSLLTQKPGPEMEELFDIGTSYGPLPESFVKSSNVSGELKAEALR
                     AEKLITKNNVIKQSLNINALDEPIPTN"
     gene            7144..7650
                     /locus_tag="PLB33_03950"
     CDS             7144..7650
                     /locus_tag="PLB33_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62708.1"
                     /translation="MDIVCLQTNAEYNSIKNEAADFKDYDCQEIFYPLWLLEVEVCMH
                     LLFSKVKRMSMKVAVDGLTGDISFICKQIETYHAEVKPEQLLPVTAKMDAEGLVKVKD
                     SLLPFVLSKARSFLVRPSINIQNKSIIFKPFYRLSSMDGDDRIIYLDKSNGKIAVLRQ
                     EGKSNDSF"
     gene            7637..8395
                     /locus_tag="PLB33_03955"
     CDS             7637..8395
                     /locus_tag="PLB33_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOG62709.1"
                     /translation="MTLFRWDELTWTELTNLRDGIIIIPTGSVEQHGPHLPIGTDTFI
                     AEGFAKKLYNKAIDKKIIIAPSISYTYAKLNSNYLGTINLNGSTLIKLGLDLISEFLR
                     QGFNKIILINGHLESIPFLMEGIELALTAYENSKNTNSEIPKIVIANWWEFISDEMID
                     NIFGDKWPGWEAEHAALTETALMKYLYPELVRDIKVDSCEYEKLPYRILPWNKRVQPS
                     SGSYANPDGATMEIGRHLTEAVISGFTELIKKEF"
     gene            8521..9684
                     /locus_tag="PLB33_03960"
     CDS             8521..9684
                     /locus_tag="PLB33_03960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOG62710.1"
                     /translation="MKHKFIAKRYWKDKSTAMGQSDVIAKSFEDIINLSLGDPDLITH
                     DIIIDNSYADAKAGHTKYTDFRGDPELRQEIINYYKEEYDMDIKDEEVFVCASGCLGM
                     YLALEAIVDDGDEIILQAPCFTPYPQQVELARGVPVELPTYEEEDFQINVDRLECLIT
                     EKTKALVINSPSNPTGSCLTVEVMEKIAQIAEKYDLIVVSDDIYTSFSYQYPFVPFAS
                     LPGMKDRTIIINSFSKNFTMTGWRIGNIIAPDYIIKVIQQINENVVFTAPSISQRAAI
                     YALKNRHIIQPPMIEEYKKRMFYAAERINKIPKLSVIYPPKGSFYLFMNIKKTGLSSV
                     EAADLILKEAHVLMLPGDAFGACGEGYIRIACTVDVETLKEAFDRIEKIALLN"
     gene            9841..11055
                     /gene="dpaL"
                     /locus_tag="PLB33_03965"
     CDS             9841..11055
                     /gene="dpaL"
                     /locus_tag="PLB33_03965"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOG62711.1"
                     /translation="MEIYEACGIKAVINNKSKGVRPYFLSEEEIKGVRKFHATIDGYE
                     ITPLVSLECLAEKLGVSKIYVKDESKRFGLNAFKGLGATYAIARLLCEKLGVDIDSID
                     YNYFKLPGVKEKIKDMVFVTATDGNHGRAVAWAAAQLGCNSIVYMPKGSSLRRLQHIK
                     DTGAEAFITDVNYDDTVRMAKKKADEIGGLLVQDTAWEGYEKVPNWIAQGYTTMAAEA
                     IDQMKSDGSMKPTHVFLQAGVGSFAGGILGYFTNIYKDEPPVTVIAEPENAACIYKSA
                     LADDGKPHSVSGDLNTIMAGLACGEPNTVTWPILRDFAKVYVSCPDYVAAKGMRILAA
                     PSGNDKKVISGESGAVGLGLLTLFLEKEELKPLKEKLKLNENSVIICFSTEGDTDPEQ
                     YRAIISQPEIFN"
     gene            11069..12460
                     /gene="argH"
                     /locus_tag="PLB33_03970"
     CDS             11069..12460
                     /gene="argH"
                     /locus_tag="PLB33_03970"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOG62712.1"
                     /translation="MKNLWGGRFSKSMEDYTADYNESISFDNILYKHSITGSKAHVKM
                     LAKQQIISNEICEEILRGLSIVEKKIENNEVEFNILDEDIMMIIEKELTKEVGEAGKY
                     LHTARSRNDQNALDETLFLRDAVKNSIKHLIDLLDVLINKAEEESHKIMPGFTHLQHA
                     QPITVGFYYMAHFQGLRRNVERFRELLKRLDKNPLGSCALAGTTLPIDRFATTELMGF
                     SEPTENALDTVASRDNIAEYLFNAALCMTHLSGFAEEIIVFNSQEFNFITIDDSFCTG
                     SSIMPQKKNPDIAELARGKSGRAIGNLITLLTVLKGTFLTLNKDYQEDKEALFDSIHT
                     LDRTVFIFARMLENITFNEDVLRAHLKKGFIEATDIAEFLVKHNIPFREAHEIVGKLV
                     KYCELKSKTFQDINKEELKELDFPVEIEDLSQFTVENCIKNRNSYGGTSYDDVKRQIN
                     NAKNFVAELKSLF"
     gene            12488..13231
                     /locus_tag="PLB33_03975"
     CDS             12488..13231
                     /locus_tag="PLB33_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOG62713.1"
                     /translation="MEAIFRKKYIVQPSETDFRRQLKLSSLFLYLQDMATEHAYNIGV
                     GRDVLQKKYGIIWVLNRVKGEVIRYPMFKEEITIETWPEPPEKVQFNRNFLVYDKDGN
                     IIVRAFSQWVVIDFITRKLRRSSIIEEEFPKVDRERAIDCTLNKIKPRGKLNLGYKKT
                     VGYSDIDINEHLNNAKYIDYIMDCFSLENHKKYSVKAIDIDYIHEALPGEIIQLYTDT
                     EDAKDNMVYIEGINENTKLTTFKAQILRK"
     gene            13357..14127
                     /locus_tag="PLB33_03980"
     CDS             13357..14127
                     /locus_tag="PLB33_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOG62714.1"
                     /translation="MISRKSVITFGMVAIPIAMYTATQDNDIHFNQLHAEDNSRIRYK
                     KTCAHCGKEITSNEIVKGYQYDKDKYVVITDEEIEKIKTEKDKSIQILHFAELNQISP
                     VYYEKTYQAVPEPGGEKAFELLRSALMAEQKIAIGKTVMGTKDTLMAIIPREEGILIS
                     TMFFADEVKEIQKQYTKPEVSKQELDMAKLLINSMDTPFDPSKYKDEYQVKLRELIEA
                     KISGREVVAAEPESAGKVIDLMEALKASVEKAKNKETA"
     gene            14129..16552
                     /gene="ligD"
                     /locus_tag="PLB33_03985"
     CDS             14129..16552
                     /gene="ligD"
                     /locus_tag="PLB33_03985"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HOG62715.1"
                     /translation="MAVGLNEYKQKRNFEKTFEPEGKTQSSEESLRFVVQHHLASRDH
                     YDLRLEWNGVLLSWAVPKGPSYNTKDKRLAVQVEDHPLEYRNFEGTIPKGEYGGGVVM
                     IWDEGYWEPHMNVDEGLSEGMLKFSLKGSRLRGRWALIRLKGKAGETQDNWLLLKEKD
                     EYSKDEEGMSEFTTSIRTGRTMAEIEGGQDEKFIKNPFSKADVQLAKLVKAIPEGDWL
                     YEMKYDGYRILAFVEGNSVRLITRKGNDITKRFFTIASSLIDLAKGRAMVLDGEIVIT
                     DESGKTDFQALQNYLKTQNSQNLTYIVFDLLAIDGEDIRGQSLILRKEKLESLLQDAP
                     KNLYYSRHVKGYGKESFAAACEAGMEGIVGKKPDSIYSGKRNDDWIKIKCDKRQEFVI
                     GGYTLSDKRSSGVSSLLLGVYEGEDLIYAGRAGTGISENDMKILESKFEGLKCSKPPF
                     KEVPKTKPNEKITWLEPKLAAEIKFAEWTKDNLLRQASYKGLREDKDTKDIKREKAEG
                     ILPQSNDEEGRYMEKDLIIEGVKISSPDKIIYNELDITKEEVIRYYSKVADKMLPYVR
                     HRILSIVRCPKGVSQACFYKKHPGPGSKGIITMPVSTGDDETGEYFYIENATGLISEA
                     QMGTLEFHIWGSSVDNLEMPDMMVFDLDPDEGMDLDKVRQGVIDIRSILDELNLKSYL
                     KISGGKGYHVVIPLKPTADWDKFHDFARQVAEVMENKWPERYTSNVRKAKRKGKIFID
                     WIRNARGATSIAPYSLRARKGAKVSMPIFWEELYTVAPDGINMEDALLRIEKKDPWED
                     FFQNSQSII"
     gene            complement(16569..17492)
                     /locus_tag="PLB33_03990"
     CDS             complement(16569..17492)
                     /locus_tag="PLB33_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOG62716.1"
                     /translation="MAIRPPILRAGDTVGVVTLGSPRDAESVDSRIQTLESMGFNVVV
                     GEFVYSDAGIVAASPMERAADLMNMFVNPEVRAIIPTRGGTGVSDIIPYLDFDVIRRN
                     PKIITGYSDITILLNVLYQLSDLITFHSLLLIDFRPTTPAYNFDQFFTATSTITAPRP
                     LENPPGIPLISRIEGNVTGTIVGGNLTSFVDTLGTPFEIDTTGRILFIEETHEAASRV
                     YRMIAHLIHAGKFRDCAGVIMGECAGCYANYGKTYEDIINEVIVPLGKPLMTNLASGH
                     GYYKMAIPIGAVTNLNTYNNTLTVLEPTVSL"
     gene            complement(17771..19123)
                     /locus_tag="PLB33_03995"
     CDS             complement(17771..19123)
                     /locus_tag="PLB33_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG62717.1"
                     /translation="MRNNIILTEGKIRSALVKLALPIMGTSFINMAYNLTDIMWLGRL
                     STDAVAAAGAAGYFLWFGSSLVMIAQVGVGVMVAQLFGKGDSEGVKNYINNGLQLNLF
                     IAILYSLFLYIFRHQIIGFFNIENENVSKMAVEYLTIISAGITFHFLNPVFSAILNST
                     GNSITPFKINTVGLIANIVIDPLLIFGFGPLPALGVKGAALATIMAQFFVTMIFLIIG
                     KRYDTIYSHAKLFKKPRLPIIQNIVKIGFPPFTQMGIHAGIAIILTRILAVFGPVAIA
                     VQSIGAQIESITWMTTEGFSAAISAFVGQNYGAGKIKRVKDGYVQGIQIVGSIGIFST
                     LLLIFGAEQLFSLFTPNDPIAIKEGIIYLHILGFSQFLMAIEIGTTGAFNGLGKTIFP
                     TINGIILNVLRIPAALILSRSLGLPGVWWAIAISSNLKGLILFILFYRYSNNFERTYI
                     "
     gene            19374..21092
                     /locus_tag="PLB33_04000"
     CDS             19374..21092
                     /locus_tag="PLB33_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOG62718.1"
                     /translation="MKRTRLKYLIISLTLILLFYIGQAASFLTEGSEKTKSINENTEK
                     AQQIIIGYYPAWKSYSGYTPESVDISKLTHINYAFANIGPDYRIEMGYPDKDPENFEK
                     FRDLKKINPDLKVLISVGGWNWSGRFSDMAATDENRNKFTDSCVEFISKYGLDGVDLD
                     WEYPVGGGLKTNSKRPEDKQNFTLLLKEMREKLDAQELKDNKDYLLTIAAGASNYYIE
                     NTEADKFQNYLDYVNLMTYDIHGPWDKYTDFNSPLYNNNDESHQYKISIESSVKAWLR
                     AGLPAEKLVVGVPFYGYLYTVSKNTIDGLYQTHKNGKALSYDTIKKEYLSNPQYTKNF
                     HYESLVPWLYNGKTFISYDDSQSIALKAEYIREQNLGGAMIWELSQDSEGELLNSLYE
                     GLYDGSVLAARDYVGHWAEAVIQKWLDMEYITGYPDGTFRPEEFITRAEFVKIVNNAF
                     GFKETAEITFTDVKEEKWYYEEIQKAYKEGDIVGVSETEFAPENYITREQAAIIMTRL
                     LELEGNEKGAVVFSDSSKVSSWAKEYVGAAAEHNLIKGYEDNTFRPQNNIKRAEALVL
                     LDRILK"
BASE COUNT         7417 a         3247 c         4425 g         6129 t
ORIGIN      
        1 cttgtgttcc tgcttttgaa taatgaagat aaaaaagccc gttgtaataa aagttagggt
       61 gaaaggctaa gcctaaaagc ccgcgctcat catatccgcc attggtcccc aactcaataa
      121 ttctctggcg aatatccaaa aaaagccttg ctattctgtt tctgatgtag aaaatctcac
      181 ctacctgagt agcaacaaat atgctctcca ttgtgtcacc cggcagtatg gcggtcttta
      241 taacggttgg taaatttata ttgctgacaa ttggtcttaa actaacatta acctttttca
      301 attattttca ctcctttata ttttttggag ggggtatctg cctcgtttaa atatatgttt
      361 acatactttt tattatgcag gcatatccct gatgtaatta aaaatgttaa ttaccaatgt
      421 ttttcaggaa ttataataaa tcttacgtaa gattctcctt ccataacagg agttaaaaca
      481 acattaaaag aggttgttgt attggggaaa atctcaatat tcattacaat aacgctgtaa
      541 tatccgggag catccactct catatggtat tcgtcagtgt ttgcttcacc caattcatga
      601 attacaggaa gttctatgat aggaattttt ccattttcat ctgtataatg gcttactatt
      661 tcattttcta tagctgattc cctgtatata cctcttaatt cataaagaga tacgctgaca
      721 cgagcacctt ctatcggatt gccttcttcc gagaataccc tcacctctaa aaaacctgta
      781 tttccacttg attcaagttt attgactttt ctatttatat atgtcctccc attaagcatt
      841 tataacatcc tttcattatt tttcaatcat tagtataata tattcaataa gaaatctttg
      901 taaacagaat acattattta tttcaaataa atgtttgcaa ccttttgttc atttcatcgt
      961 caaaaatgtt gggagagtgt tatggtagca gaagaattat tgatattgaa cttaaaaaga
     1021 ggtcgtgaag aggcttacag acaattaata gaaaaatatg gcaatagatt gtatagaacc
     1081 tgctacctga tactaaacga cagagaggaa gcagaagatg ttgttcaaga aacatttata
     1141 aaggtattta agaaaattga tacatttaag ggacagtcag ggctttatac atggatttat
     1201 gcaattgctc tcaacctctc aagagacaga ataaggatga aacaggatat gctcgagttg
     1261 aaagatgagt ggattggaag ttatgatgtt gaatctctgg tagaaataaa tatcgacaga
     1321 gagctattga aaaaagaaat atttgaaatg aattcacttt atagagaggt acttgtgcta
     1381 ttctactttg aagagctttc tataaaagaa atttcaaatt tattgaatga aaaggaagga
     1441 acaattaaaa gcaagttatc aagagggaga aatatattga aggaaagcct tttgaaagga
     1501 ggcaggtaat ggataagaaa gataaattag gaatagaaat aaaaagtatt atggaagaag
     1561 aaacgcttga ccttactctg tctcaaaaag cctttgataa tattattcaa cacagaaaga
     1621 agtctttaag agaaaaaatt caggagttta taaacaaaga gattgaaata cccgtagcac
     1681 ccgcaataat agggtttgca gccttgttag ctgttacatt gattccgatg gatatattca
     1741 aatcacaaaa tgaaagaatt atcgacatgg gcgggtctta tgtaataatt agggaaggtt
     1801 ttgaggtgag caagaaatga agataaaaat taaaattaaa acattaattt ttgcagtatt
     1861 tatacttacg ttgactttta tatgggcaat accgacagca accttaatga ttgcaggata
     1921 tcttgagact aaagattccg agaaagcttc tcttttttac gaaaaatatg catcctatcc
     1981 gaccacgcca agtatagaaa gcaagtatct tcatgcaaac agcctgataa ggagttttaa
     2041 caagtttacg atttttctga caggctgggg cggaggtgaa aatacaagcc ctgaagacat
     2101 agaaaaagct aaacaaattc ttatggaaac attagcggaa aaacccgtta aaaacagaga
     2161 aaaagattat tatataaaat catataaaat gcttcttgat attttgattg catcaggaga
     2221 tgcagaaacc cttcaagaat ggatagcctt tggacaaaaa ggtacagatg aaagattagt
     2281 atatactgct gatgtatatg atggatttct tcatcacgta aatggggaag gtgaagcggc
     2341 aaagtcaatt attgaaaaat atgatgagac agaattaaaa gacactatct tttatgtatt
     2401 aaaagcagaa attgctttat ttgatggaaa atatgaagaa tcggaaaaaa tatatgataa
     2461 tatatataaa aataattgga gagaattagg aaaaagcttc ggttccggag gatattacag
     2521 aaatgattgg tttgacagat ttatggaaga cttcaaagga gataatgtca taacagggac
     2581 tgtaacctgt gaaggaaagc ccatgccctt tgttgaaata tatgtgcagg aagccaaaga
     2641 aggatttagt ttttacgcca gcggagaaaa ctatgttgct ataaccgatg taaacggaga
     2701 atttaaaacg cttggtttaa aagacggatt atacagtgtg ggaatcggag ttgacggctc
     2761 tgtcctggca aacaaggcat ttcaaggttc aacgatgcag tatgttgagc tgaatggagg
     2821 agatggcgaa tttcaccttt cattcagaaa taccctaaaa atatattcac ctgagccggg
     2881 agaaaagcta tcaggagaag aatttacagt atcatgggaa gagattgaag acgcagccta
     2941 ctacaaggtt gaaccggtgg ttttttatga gccgttcggg gagagaggaa taagcacacg
     3001 tatatatgca aaagacaaga atggagaata caaatttact gaaaatcaag ctgtatttaa
     3061 cataaatatg attagaaaca acccatatat tactgtaagt gattcggata acggatacct
     3121 tggagctcag gcggttcttg gcatattcat tccgggagtt gaatatccca ttgttgtaaa
     3181 tgcatatgat gagaataaca atctcataac cggcagcctt cctttaaggt cttattacga
     3241 gcatattcca agtattacgg cagaaggaag tttgaatgaa ggagaaaatt ttatttatcg
     3301 caaaaactat cctgcagcta ttgaatacta cgaaaatatt ctaaaagaaa atcctgatga
     3361 tatagatgcg ttaagatacc ttacaaggat atacggaatt ggctggaagg agggagagca
     3421 aaattttgaa agagctttta ctttggggca gagatatact gaaatttcag gaaacagaga
     3481 attgctgacc aatacccttg gtgggatgaa catcagtgaa ataaaggaaa acagagagaa
     3541 tgtttatttg actctaaaag aagctatgaa ggaaccggat tttgataact attatttttt
     3601 aagcagatat tatattgctg ttgaaaatta tgaaggtgca agagaagctt tgcaaatggc
     3661 agaaaacgta acggataatt tgattttctt aaacatgtat tttggagact ataaggaggc
     3721 tgttgaaagt ataaaatcgg aaagattcta caggtcaagg ttgtcatcaa ataaagtaaa
     3781 agatgccatt gaggctcttg atgaaagtcc tccaagggga gatgaattaa aggtatttaa
     3841 tgacttcttg ctaaaactga taaacggcat tccatatggt gaaaaccgga atatttataa
     3901 taaaaccgta aagcaaattt caaacagcaa tttaaaaact attattgatg aaatttatct
     3961 tgatagaaat tgggacttag agtactaatc attttgcttt acataattaa atttatgtaa
     4021 agtagaatta ttgcgtaatg atataaattg tgtgatataa tatgtatcta aagaaaggat
     4081 attgtgcggg aggtatcaaa tgaagaaatt tataatcgag gatgattttt gggaattgtt
     4141 ccctgatgca aaatttggag ttgttatatg tcataatata gacaacaaaa taaaagaagt
     4201 ggataaatat ttggatatgc ttagagattc cgaggatgaa gcattaaaat acctggacaa
     4261 tcctgagttt atacaaaacg atgttataaa ggtatggagg gaggcatttc aaaaattcaa
     4321 aacaaagaaa ggtgcaagat gctccattga ggccttgttg aagagagtaa aaaacgggaa
     4381 tcatataggt actataaatc cattagttga tatttacaat tctatttcat tgagatatgc
     4441 attgccctgc ggcggtgagg atattgatgc atttgcggga gataacagat tgacaaaggc
     4501 agtcggagac gaaagtttta ttacatacgg ctcggataaa agcgagccgc cctacgaagg
     4561 tgaaatagtt tataaagatg accaaggggc aatttgcagg tgctggaatt ggagagaatc
     4621 agtaagaacc atgcttacag aaagtacaaa aaatgctttt ttggttattg aatcggttga
     4681 tgaaaaaaga acggaagaat taaaaaatgc cataaatgat ttagcaaaac tggtacagga
     4741 taatttaggc ggtacaatta aaacatcaat tcttgatatt aacaataagt tgattgattt
     4801 agaataattc taccaatatg cagatcaatg atataattga aaataacctt attatttttt
     4861 tacctatata aaaaaacaaa taataaaaaa aatatgttga cattataaat aaaccaactt
     4921 ataatttata aaaataataa actatgtata atttaatgat tggaagagta gattaaataa
     4981 agcttcagcg agccggagca gagtgtgagt tcggtagtgt agtttaattg aaaagagtcc
     5041 atgaaaagat tctcagaagt agcagtatga gaatacggtt aaaatccgtt attatgaaag
     5101 agagaagcag ctgcttcata aaagagtggt accgcggtgt aaacactgcc tctttatggg
     5161 cagttttttt ttgcccttta gaaaccaaga ttcttcatat ggggatgaaa ggcatgcata
     5221 aatattgata ataattccgg agctttacac atataggttt atagataagt aattatcttc
     5281 agatatatat ttcaaattta gtgcagtaat cttggacaaa gccgcaagtt tatttaaaga
     5341 aggaggtgaa aagatggaag gttcaaaact tttttggtca tcaggaacat ggattttttt
     5401 ccttgttcag gcgttagtag ttgctctggc agtctatttg tattggtatt tgggaaagaa
     5461 agaaaaggaa gcatgtgaag aaaaaaaaga ataagataaa gctaaattaa aggaggcaaa
     5521 aaaatgaaat taaatgtaat aataccattt gtatcttatc tgttaataat gcttggaata
     5581 ggcatgtatg caatgcgacg aatcaagact tatgatgatt ttattattgg aggaaggtcc
     5641 ataggagcat gggtttcatc ctttgcactt attacatctt atgccagcgg ttatacttat
     5701 actgctgctc ccgggatgtc ctatacggga ggatggtcta ccatgtggtg gtcaaccggt
     5761 gatgctcccg gaaacgcact atcatttgga ttacttggaa gaaggcttag gaagttttcg
     5821 gagttgctgg gagctattac cttgcctgaa tattacgaaa agcgtttcaa aagcccggga
     5881 ttaagattgt tgacttctat tataattatt actactgtaa gtctgcacct tgtagcacaa
     5941 tggcaggcat ccggaatatt gctgagtgta acttttggaa cagattattt agttggaatg
     6001 cttataggcg gtgcagtggt acttgcttat acaataatgg gaggatatct ggccactgtt
     6061 tatactgatt ttatgcaggg agtggtgatg tttatcggta ctcatattct attttggagt
     6121 gcaatttcta aagtaggcgg gtttaacgca ttaaatgacc agttggcttt aataaacccc
     6181 ggtttggtta caccttgggg acctgacggc tcatatttcg gccttattgc agcagcttct
     6241 cctataatac ttattataat ggggtcattt ggaatgcctc atgtaaccgt acgccattta
     6301 tcccttaaaa accctgatac ggcaaggaaa gcaatgctta taactgcaat atttgttgca
     6361 ctcttttctt ttgcatatta catggtggga gctgccggat tgattttatt gggacccggt
     6421 ttggaaaacc ctgagacatc aggtgttttg ctttggtttg aagtgcttcc gccggttgga
     6481 gccggtatcc ttgtttctgc agctgttgca gctattatgt ctacagcaga tggattcttg
     6541 atgcttatag tttcaaccat tgctcatgat atattaaata gatttttact tccaaatacg
     6601 cctcaggaga aaagaatccg tattgctcgt ataattacgg caatcattgt aattctttca
     6661 cttgttgctg caataaatcc tccggcagga gtctttacca tagtaatgac agtattcgga
     6721 ggcatggctc ttgcttttgg tattcccaat atcttctcgg tttattggaa gcgcagtacg
     6781 aaaaccggtg caattgcttg tgttattttg tcattgacgg tatatgtatg gcttacagct
     6841 gccggaatag agttgtttgg tatgagtccc ttcatgaatg gattaattgt tgctatatta
     6901 gcatttatta ttggaagtct gcttacacaa aaaccaggtc ctgagatgga agagcttttt
     6961 gacattggaa cttcttacgg accgcttccg gaatcttttg taaagtcatc aaatgtttcg
     7021 ggggaattaa aagctgaagc tttgcgtgca gaaaagctta taactaaaaa caatgttata
     7081 aaacaatcat tgaacataaa tgctttggac gagccgatac caactaatta ggcagtgata
     7141 actatggata tagtatgctt acaaactaat gcagaataca attcaataaa aaatgaagca
     7201 gctgatttta aagattatga ctgccaagag atattttatc cgttatggtt gttagaggtt
     7261 gaagtttgca tgcatttact tttttcgaaa gtaaaaagga tgagtatgaa ggtagccgtg
     7321 gatggattaa ccggtgatat aagttttatt tgtaaacaaa tagagacata tcacgcagaa
     7381 gtaaagcctg aacaattact gcctgtaacc gcaaaaatgg atgcggaagg attggtaaaa
     7441 gtgaaagatt ctctgctgcc ttttgtacta tctaaagcac ggtcgtttct tgtcagacct
     7501 tcaattaaca tacaaaacaa aagtattatt tttaagccct tttacaggct ttcatctatg
     7561 gacggggatg acagaatcat ttatttagat aagagtaacg gtaaaatagc cgtattaaga
     7621 caggagggaa aaagcaatga ctctttttag atgggatgaa ttaacatgga cagaattaac
     7681 caatcttcgt gatggcatca ttatcatacc gacaggctcg gttgaacagc atggaccgca
     7741 tttgcccatt ggtacggata catttatagc tgaaggtttt gctaaaaaat tgtataataa
     7801 agctatagat aaaaaaataa taatagctcc ttctatatca tatacttatg caaaactaaa
     7861 cagtaattat ttaggaacaa tcaatttgaa cgggagtaca ctgattaaac tgggcttaga
     7921 ccttatttcg gaattcttga gacagggatt taataagatt attttaataa acgggcatct
     7981 tgaaagtata ccttttctga tggaaggaat tgagctggct cttacagctt atgaaaattc
     8041 aaaaaataca aatagtgaaa ttcctaaaat agtaattgct aattggtggg aatttatatc
     8101 tgatgaaatg atagataata tatttgggga taaatggccg ggctgggaag cggaacatgc
     8161 tgctttaacc gagacagcac tgatgaaata tttataccct gagttggtta gggatataaa
     8221 agtagattcg tgtgagtacg agaaattgcc ttatcgcatt ttaccttgga ataagcgtgt
     8281 tcaaccttca tccggctcat atgcaaatcc tgacggtgct actatggaaa ttgggagaca
     8341 tctgactgaa gcagttattt caggttttac agaactaatt aaaaaagagt tttaatgagt
     8401 tacctataat aagaatgttt atatatgctg aatactatgt taaaatatgg gtaaaagtgt
     8461 agatgaataa caaatctgcc aatcagttaa aataagaggc atagtaaatg gaggatagta
     8521 atgaaacata agtttattgc aaagaggtac tggaaagata aaagtacagc catgggtcag
     8581 tctgatgtga tagcgaagtc ttttgaagat attataaatt taagtctggg tgacccggat
     8641 ttaattacac acgatattat aatagataat tcttatgctg atgctaaggc aggacataca
     8701 aaatataccg actttcgcgg ggaccctgaa ttgcggcaag aaataattaa ttattacaaa
     8761 gaagaatatg atatggatat taaagatgag gaagtctttg tttgtgcatc gggatgtctt
     8821 ggaatgtact tagccttaga agctattgtt gatgatggag atgaaataat actgcaggca
     8881 ccatgcttta cgccatatcc ccagcaggtg gaattagcaa ggggagtacc cgttgaactg
     8941 cctacttatg aagaagaaga ctttcaaata aatgtagata gacttgaatg cttaattaca
     9001 gaaaagacaa aggcattggt tattaactca ccaagcaatc cgaccggctc ttgtcttact
     9061 gttgaagtta tggaaaaaat tgcacaaatc gctgaaaaat acgacttgat tgtagtttca
     9121 gatgatattt atacatcttt cagttatcaa tatccatttg tgccgtttgc aagtcttccg
     9181 ggcatgaaag acagaacaat tataattaac tctttttcaa aaaattttac tatgaccggt
     9241 tggagaatag gaaatattat tgcaccggac tatataataa aagttatcca gcagattaac
     9301 gaaaatgtag ttttcacagc accgtcgatt tcgcaaaggg ctgcaatcta tgctttaaaa
     9361 aatcgccata taattcaacc gcctatgatt gaagaatata agaagagaat gttttatgca
     9421 gctgagcgaa taaataagat tccaaaactt tctgttattt atccgccgaa gggaagcttc
     9481 tacttgttta tgaatattaa aaaaacagga ctgtcaagcg ttgaagcggc agatttaatt
     9541 ttgaaagaag ctcatgtatt gatgctgccg ggggatgcct ttggagcttg cggagaaggt
     9601 tatattcgca ttgcctgcac agttgatgtt gaaacactga aagaagcatt cgatagaatt
     9661 gaaaagatag cattattaaa ttaatataaa gtatttatag ggaatataaa aacttcccat
     9721 aataatattt gggataacca aaaggttatc caatattttt tcggcaggaa aatattgacc
     9781 ttaatataca cttattatat agtaatgtta gcgttctact actaattatg ggggtatgta
     9841 atggaaatat atgaagcatg cggaatcaaa gcagtaataa ataataaaag taaaggtgtg
     9901 aggccttact ttttaagtga agaagaaata aaaggagtaa ggaagttcca tgctacaatc
     9961 gacggctatg aaattacgcc tttggtaagc ttggaatgtt tagctgaaaa gttaggtgtt
    10021 tccaagatat atgtaaagga tgaatcaaaa agatttggac ttaatgcatt taaaggcttg
    10081 ggtgcaacat atgccatagc aagactgttg tgcgagaaat taggtgtgga tatagattca
    10141 atagattata actactttaa gctgcctggg gtgaaagaaa aaatcaagga catggtcttt
    10201 gtcaccgcaa cagacggtaa tcatggaaga gcggtagctt gggctgcagc tcaattggga
    10261 tgcaactcaa ttgtatatat gccaaagggg tcttccctga ggagattgca gcatattaag
    10321 gataccggtg cggaagcttt tattacggat gtaaattatg acgataccgt aaggatggca
    10381 aagaagaaag ctgatgaaat aggtggatta ttagtccagg atacagcttg ggaaggatat
    10441 gaaaaagttc ccaactggat tgctcaaggc tatactacaa tggcagccga agccatagac
    10501 cagatgaagt cggatggaag catgaagccg acgcatgtct ttttacaggc gggagtgggc
    10561 tcctttgcgg gaggtatatt gggatacttt acaaatattt ataaggatga gccgcccgta
    10621 actgtaattg cggagccgga aaatgccgca tgtatttata agtctgcctt ggcggacgac
    10681 ggaaaacctc attccgtttc gggagacctt aataccataa tggcgggatt agcttgcgga
    10741 gagcctaata cggttacctg gcctatttta agagattttg caaaagtcta cgtgtcttgt
    10801 cctgattatg tggcagctaa aggaatgaga atattggcag ctccttcagg taatgataag
    10861 aaagtcatat caggagaatc aggtgcagtg gggttaggac tgttaaccct gtttcttgaa
    10921 aaagaagagt tgaaaccatt aaaagaaaag ttaaaattaa atgaaaactc tgtaataata
    10981 tgtttcagta cggaagggga tactgacccg gaacagtacc gagcgattat ttcacagccg
    11041 gagattttta actgaaagga tgaaaagtat gaaaaattta tggggcggtc gtttttccaa
    11101 gagtatggaa gactataccg ctgattacaa tgaatctata agctttgata atattttgta
    11161 taaacacagt attacgggca gcaaagctca tgtaaaaatg cttgcaaaac agcaaatcat
    11221 aagcaatgaa atatgcgaag aaatattgag gggattatca attgtagaaa agaaaattga
    11281 aaataatgaa gttgaattta atatactcga tgaagatata atgatgatta ttgaaaaaga
    11341 gctcaccaag gaagtcggag aagcaggaaa atatcttcac acagcgagaa gcagaaacga
    11401 ccagaatgca ttggatgaaa cgctgttttt gcgggatgca gtaaagaatt caatcaaaca
    11461 tttaatagat ttacttgatg tcctgataaa taaggcagaa gaagaatcac ataaaataat
    11521 gcccggtttt actcatttac agcacgctca gccgataacg gtcggattct actatatggc
    11581 acattttcaa ggcttgcgaa gaaatgttga gagattccga gaattattaa aacgtctgga
    11641 caaaaatccc ttaggatcgt gtgcattggc gggaaccaca ttgcccatag accgttttgc
    11701 gacaacagaa ttaatgggtt tttcggaacc cacggaaaat gcattggata cagttgcttc
    11761 aagagataac attgcagaat atttatttaa tgctgctcta tgtatgactc atttaagcgg
    11821 ttttgcagaa gaaattattg tttttaattc tcaagaattt aatttcatca ctattgacga
    11881 cagcttttgt acgggtagca gtattatgcc tcagaagaaa aacccggata ttgccgaatt
    11941 ggctcgaggc aaatcaggta gagccatagg gaatctcata acacttttga ccgttttaaa
    12001 aggtaccttt ttaacattaa acaaggatta tcaggaagat aaagaagcac tctttgacag
    12061 tatacataca ttggacagaa cagtgtttat atttgccaga atgcttgaaa atataacttt
    12121 taatgaggat gtattaagag cgcatttaaa aaaaggattt atagaagcaa cggatatagc
    12181 ggaattcttg gtaaaacata atattccatt tagggaagct cacgaaatcg tgggtaagtt
    12241 agttaaatat tgcgagctca aatcaaagac ttttcaggat ataaacaaag aagaattgaa
    12301 ggagttagac tttcccgttg aaattgaaga cttgagtcaa tttaccgtag aaaactgtat
    12361 aaagaacaga aacagctatg gaggcacatc ctacgatgat gttaaaagac aaatcaataa
    12421 tgccaaaaat ttcgtcgctg agttaaaaag tctgttctaa tcatcctgag tatgatagag
    12481 gtgtaagatg gaagcaattt ttagaaagaa atacatagta cagccgagtg aaacagattt
    12541 tagaaggcag cttaagttaa gctcgttatt tttatacctg caggacatgg caaccgagca
    12601 tgcctataat atcggtgtgg gaagagatgt attacaaaaa aagtacggca tcatatgggt
    12661 attaaatcgt gtaaagggtg aagtaataag atatcctatg ttcaaggaag aaataacaat
    12721 tgaaacttgg ccggagccgc cggaaaaagt tcagtttaac aggaattttt tagtttatga
    12781 caaggatgga aatattatag tcagggcttt tagccaatgg gttgtaatag atttcattac
    12841 taggaaactg agaagaagca gtataataga agaagaattt ccaaaggttg acagagaaag
    12901 agctatcgac tgtacactga acaaaataaa accaagaggc aaactaaatt tgggttataa
    12961 aaaaacagtc ggttacagtg acattgatat aaacgagcat ttgaacaatg caaaatacat
    13021 cgattatata atggactgtt tcagcttgga aaatcacaaa aaatactctg tcaaagcaat
    13081 cgatatagat tatatacatg aagcattgcc gggtgaaatc atacagcttt ataccgatac
    13141 agaagatgca aaagataata tggtatacat agaaggaatt aacgaaaata cgaaacttac
    13201 aaccttcaag gcacaaatat taagaaaata attacaaaag acacgggacg tttgttttgc
    13261 ttccttgtct tttttatata cgattgatag ccaatacttg tcagtatgtc gaatacataa
    13321 aatataattg gcaacaataa ccttggaggt gattatgtga tatcacgtaa atcagttata
    13381 acttttggca tggttgctat tccaattgca atgtatacgg caacgcaaga caatgatatt
    13441 cattttaatc agctgcatgc ggaagacaac agccgaataa gatacaagaa aacatgtgca
    13501 cattgcggca aagaaataac ttctaatgaa attgtaaaag gataccaata tgataaggac
    13561 aagtatgttg taatcacaga tgaggaaatt gaaaaaataa agacggagaa agataaatca
    13621 attcaaattc ttcattttgc agaattgaac caaatttcac ccgtttatta tgaaaagacc
    13681 tatcaggcag tgccggagcc gggcggggag aaggcttttg aactgttgcg ctctgcactg
    13741 atggccgagc aaaaaatagc cataggaaag actgtcatgg gcaccaagga cacattgatg
    13801 gctattattc ctcgagagga aggtatattg atttctacca tgttttttgc cgatgaagtc
    13861 aaagaaattc aaaaacaata taccaagcct gaagtatcca agcaggagct cgatatggca
    13921 aagcttctta taaattcaat ggatactcct tttgaccctt ccaaatacaa ggatgaatac
    13981 caagtgaagc ttcgtgagct tattgaagca aaaatttcag gaagagaggt tgttgctgcc
    14041 gagcctgaaa gtgcaggcaa ggtaatcgac ctgatggagg ctttgaaagc cagtgtagag
    14101 aaagctaaga ataaggaaac ggcataatat ggctgtagga ttaaatgaat ataagcaaaa
    14161 aagaaatttt gaaaaaactt ttgagccgga aggaaagaca caaagctcag aagaaagttt
    14221 aagatttgtt gtccagcatc atttagccag cagagaccac tatgatttgc gtttggaatg
    14281 gaatggtgtt cttctaagct gggcagtacc caaggggcct tcttacaata ctaaggataa
    14341 aaggcttgcc gtacaggtag aggaccaccc tttggaatac aggaattttg aaggtaccat
    14401 tcctaagggg gaatatggcg gcggggttgt aatgatttgg gatgaaggat actgggagcc
    14461 tcatatgaat gtggatgaag gtctcagtga aggtatgctc aaattcagtc tgaaaggaag
    14521 ccggcttaga ggcaggtggg ctttgataag gttaaaaggt aaggcaggtg aaacacagga
    14581 taactggctt ttattaaaag aaaaggatga gtattctaag gatgaagaag gaatgtccga
    14641 atttaccacc agtatcagga ccggacgaac aatggctgaa attgaaggag gtcaagatga
    14701 aaagttcatc aaaaatcctt tcagtaaagc tgatgttcag cttgccaaat tagttaaggc
    14761 aatcccggaa ggtgattggc tttatgaaat gaaatatgac ggctatagaa ttttggcatt
    14821 tgtggaaggc aacagtgtcc gtcttataac aagaaagggc aatgatatta ccaaacggtt
    14881 ttttacgata gcctcttcgc ttatagactt ggctaaggga agagcaatgg tattagacgg
    14941 tgaaatagtt attacggatg aatcgggcaa gacggatttt caagctttac aaaactattt
    15001 aaaaacgcag aactctcaaa atcttactta tattgtcttt gatttgctcg ccattgacgg
    15061 agaggatatt cgaggacaat ctttaattct aagaaaagaa aagcttgaaa gtttattgca
    15121 ggatgcgcca aaaaatcttt actacagccg ccatgtgaaa ggatacggaa aggaaagctt
    15181 tgctgcagct tgtgaagctg gaatggaagg aatagtgggg aaaaaacctg attccattta
    15241 cagcggaaaa aggaatgatg actggataaa aattaaatgc gataaaagac aagagttcgt
    15301 tatcggagga tatacacttt cagataaaag aagcagcgga gtaagctccc ttcttctcgg
    15361 agtttacgag ggtgaggatt taatatatgc aggacgtgca ggcacgggaa tcagtgaaaa
    15421 tgatatgaaa attcttgaga gtaaatttga gggtttaaag tgctcgaagc ctcctttcaa
    15481 ggaggtgcca aagacaaagc ctaatgaaaa gattacctgg cttgagccca aattggctgc
    15541 agaaataaag tttgcagaat ggaccaagga taatctgcta aggcaagcaa gctacaaagg
    15601 attaagggaa gataaggaca ctaaggatat taaaagggaa aaggcagagg ggattctgcc
    15661 ccaatcaaat gatgaagagg gaagatacat ggaaaaagat cttattattg aaggagttaa
    15721 aatttcaagt cctgacaaaa tcatatacaa tgaactcgat ataacaaagg aagaagttat
    15781 tcgttactat tcaaaggttg cagataaaat gcttccttat gtgaggcata gaattttaag
    15841 tattgtacgt tgccccaagg gagtatccca agcgtgcttt tataaaaagc accccggtcc
    15901 cggaagcaaa ggaattatta caatgcccgt ttcaacaggc gatgatgaaa caggtgaata
    15961 cttttatata gagaatgcaa cagggttaat atctgaagcc cagatgggta ctttggaatt
    16021 tcatatttgg ggaagcagtg ttgataatct tgaaatgccg gacatgatgg tatttgattt
    16081 ggacccggat gaaggaatgg atttagacaa agttcgccaa ggggttattg acattagaag
    16141 tatacttgat gaactaaatt taaaatcata tcttaagata agcggtggta agggatatca
    16201 tgtggtaatt ccgttaaaac ctaccgccga ttgggataaa tttcatgatt ttgcaagaca
    16261 ggttgcagag gtaatggaaa ataagtggcc ggaaagatat acaagcaatg taagaaaagc
    16321 taaaagaaaa ggcaaaatat ttattgactg gattcggaat gcaaggggag ctacaagtat
    16381 tgccccttat tcactgaggg caagaaaagg agctaaggtg tctatgccta tattttggga
    16441 agagctttat acggttgcgc cggatggcat caatatggag gatgctcttt taaggattga
    16501 aaaaaaggac ccttgggagg atttcttcca aaacagccaa tcgattatat agaataacct
    16561 gcaagaacct acaggctcac tgtaggttcc aatactgtca atgtattatt ataagtatta
    16621 agattagtaa ctgcccctat gggtatggcc attttataat agccgtgccc tgaagctaaa
    16681 ttggtcataa gaggtttgcc taagggaaca attacttcat ttattatgtc ttcataggtt
    16741 tttccgtaat ttgcataaca acctgcacat tctcccatta taactccggc gcaatctctg
    16801 aatttacctg catgaatcaa atgagcaatc attctgtaaa cacggcttgc tgcttcgtga
    16861 gtttcttcaa taaataaaat ccttccggta gtatcaattt caaagggtgt tccaagtgta
    16921 tcaacaaaag acgttagatt gcctcctact atggtacctg ttacatttcc ctcaattctg
    16981 cttatcagcg gaataccggg gggattctcc aaaggtcttg gtgcagttat tgttgatgtg
    17041 gcagtaaaaa attggtcaaa attatatgca ggggtagtgg gtctaaaatc tattaacaat
    17101 aagctgtgaa aggtaattaa gtctgacaat tggtataaaa cattcaataa aattgttata
    17161 tcgctgtatc cggttataat cttagggttg cgccttatta catcaaaatc aagataagga
    17221 atgatatccg aaactcccgt accacctctt gtaggtataa tagctctaac ttcgggattc
    17281 acaaacatat tcattaaatc cgcagcacgt tccatgggcg aagcagcaac aatacctgca
    17341 tcggaatata caaattcgcc tactacaaca ttaaaaccca tagattctaa tgtttgaatt
    17401 ctagagtcaa cagattcggc atcacgagga ctacccaagg taactacgcc tacggtatct
    17461 cctgctctta aaataggtgg tctgatagcc attctttcct cctaataata tccattctat
    17521 ttagttatta tatgagaaaa agctatgttg gttaataaat aagaagttca tcggcagaag
    17581 aaaaaatcaa agcatatatg aaattgtaaa ttgaatagct caaaaattca attaatggtt
    17641 atcttgatat aaaagatact tctacaggtc atatatgctc gaaattctaa ttaatgtgtc
    17701 taaaatggaa taaattaatt ttttaattca cacattaatt gaaattttga acactcagat
    17761 gaaaaaaata ctaaatatag gttcgctcga aattattcga gtatctataa aataaaataa
    17821 ataatattaa tccctttaaa tttgaagata tagctattgc ccaccaaaca cccggcaatc
    17881 ctaatgacct gcttagtatc aatgcggccg gtattctaag tacattcagt ataattccgt
    17941 ttatcgtggg gaaaattgtt tttcctaatc cgttaaatgc accggtagta ccaatttcaa
    18001 ttgccatgag gaattgagaa aaacctaata tatgcaaata aattattcct tcctttattg
    18061 caatcgggtc attgggagta aataatgaaa atagttgttc agcaccaaaa atcaatagca
    18121 aggttgaaaa tattccgata ctccctacaa tttgaatccc ctgtacatat ccgtctttga
    18181 ctcgttttat ttttccggca ccgtaatttt gccctacaaa tgcagatata gctgcagaaa
    18241 aaccttcagt tgtcatccat gtaatggact cgatttgagc gcctatactt tgaacggcaa
    18301 ttgcaacggg tccgaaaact gccagtattc tggttagaat tattgctatg cccgcatgga
    18361 tacccatctg agtaaaggga ggaaatccta ttttgactat attttgaata atgggtagtc
    18421 ttggtttctt aaataatttt gcatgtgaat aaatagtatc atatcttttc cctataatta
    18481 agaaaatcat cgtcacgaag aattgagcca ttattgtagc taaggcagca cctttaacac
    18541 ctaatgccgg caagggaccg aaaccgaata tcaacaaagg gtcaataaca atatttgcaa
    18601 taagacccac tgtattaatt ttaaaaggag taatactgtt tccggtgctg tttaaaattg
    18661 ctgagaaaac aggatttaaa aagtgaaatg tgattcctgc tgaaataatt gtcaaatact
    18721 caactgccat tttacttaca ttttcatttt caatattaaa aaaaccaatt atttggtgtc
    18781 tgaaaatata caaaaataaa gagtataata tagctatgaa aagatttagc tgaagtccgt
    18841 tgttgatata gttttttaca ccttccgaat ctcccttccc aaataattgg gcaaccataa
    18901 ctccaactcc cacctgtgca atcatcacca atgaagagcc aaaccataaa aaatagcctg
    18961 cagcaccggc ggctgcaacg gcatcggtac tgagcctgcc aagccacata atgtctgtca
    19021 agttgtatgc catatttata aatgaagtgc ccattatggg aagtgctaac tttactaatg
    19081 cacttcgtat ttttccttct gtcaaaataa tattgtttct cattcttttg tccttagtaa
    19141 ttattattgt attattttaa ctgataattt tgctttgtac aagcaaaaca tatacttgcc
    19201 aataaggcaa aacaaaatta cttcacgatt ggttacttat aatggaaaat aagctaagag
    19261 tataaggaat tgcctaatat ttcaagattt ataacaatat tattatgtta taattaaaaa
    19321 aacgttttat aggaggagta tgatgtatat tgtctataca tcatacagga tatatgaaga
    19381 ggacaagatt aaagtattta atcatatcat taaccttaat tcttttgttt tacataggac
    19441 aagctgcatc ttttttaacc gaaggaagtg aaaaaacaaa gtcaataaat gaaaatactg
    19501 aaaaagctca acaaataatt atagggtatt atcctgcatg gaagtcttat tcgggatata
    19561 ctccggaaag tgtagatata agcaaattaa cccatataaa ttatgcgttt gcaaatatag
    19621 gtccggatta cagaatagaa atgggatatc cggataagga ccccgaaaat tttgaaaagt
    19681 ttcgggatct taagaaaatt aaccctgatt taaaagtatt aatttcagta ggcggatgga
    19741 attggtcggg gagattttcg gacatggcgg ccacagacga aaacaggaat aaatttacgg
    19801 acagctgtgt tgagtttatt tcaaagtatg gtcttgacgg tgtggatttg gactgggaat
    19861 atccggtcgg aggaggcctt aaaactaatt caaaaagacc tgaagacaaa caaaatttca
    19921 ctttgcttct taaggaaatg cgcgaaaagc tggatgctca agaattgaag gataacaaag
    19981 attatttgct cactattgca gccggagcaa gcaactatta catagaaaac acagaagcag
    20041 ataaatttca aaattatttg gattatgtaa acctaatgac ctatgacata catggtcctt
    20101 gggataaata tactgacttt aattctccct tgtataacaa taatgatgaa tcacatcaat
    20161 ataaaataag cattgaatca agtgttaagg catggctaag agcaggtttg ccggctgaaa
    20221 agctggtagt gggagttccg ttctacggtt atttatacac ggtatcaaaa aatactattg
    20281 atgggctcta tcaaactcat aaaaacggca aagcattgag ctacgatacc ataaaaaagg
    20341 aatatctcag caaccctcaa tatacaaaaa attttcatta tgagtcattg gttccatggc
    20401 tttacaacgg aaaaaccttt ataagctatg atgattctca gtctatagca ctgaaggctg
    20461 aatatataag agagcaaaat ttaggcggtg caatgatttg ggaattaagc caggattcag
    20521 aaggcgagct tttaaattca ctgtatgaag gtctctatga cggctcggtg ttggcggcaa
    20581 gagactatgt gggacattgg gcagaagccg taatacagaa atggcttgat atggaatata
    20641 taaccggata tcctgacggg actttcagac ctgaagaatt tataacaaga gccgagtttg
    20701 tcaaaatagt taataatgcc tttggtttta aagaaactgc cgaaattaca tttacggatg
    20761 ttaaagaaga gaaatggtac tatgaagaaa tacaaaaggc atacaaagaa ggagatatag
    20821 taggagtttc ggaaacagag tttgctcccg aaaattatat tactagagag caggcagcaa
    20881 taattatgac aagactgctt gaattagaag gaaatgaaaa aggtgccgtt gtattctccg
    20941 acagcagcaa agtcagcagc tgggcaaagg aatatgtagg tgcagcggcg gagcataatt
    21001 tgattaaggg ttatgaagac aatacattta gaccgcaaaa caatataaaa agagccgaag
    21061 ctcttgtctt gctcgacaga atactgaaat aaaaaggtgc cataataaaa aggtgccagg
    21121 cacctttttc gtgccaggta ccttttttat cattgctaat aatcaaaaat tgtactacaa
    21181 tatagtcagg tatattttat gttcctaaaa tttaagtt
//
LOCUS       DAOSHP010000025        20922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2062_length_20922_cov_10.485695, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000025 DAOSHP010000000
VERSION     DAOSHP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 20922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20922
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2062_length_20922_cov_10.485695"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLB33_04005"
     CDS             <1..482
                     /locus_tag="PLB33_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG62719.1"
                     /translation="MDGDGPSNGRVNNFGIMLACKSSFELDYVIEKLIGTSEPMPISD
                     MALKHNLIKKYSACCVYPFSNDIDVCKKCFVDFKFPKTINTDWRLPVFIKTTLSKIFI
                     KRPKINKNRCRNCQICLNVCPVKAISNSGITPNINYKKCISCYCCQEMCPESAITIS"
     gene            complement(1551..2465)
                     /locus_tag="PLB33_04010"
     CDS             complement(1551..2465)
                     /locus_tag="PLB33_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOG62720.1"
                     /translation="MINKFKFLYFVIFTTVFVNMLFDKFDTFAFNQDDYIKLITTNTC
                     QNCDLFRLDLTHKDLTASILDNAKMTDTDLTNTDFYFSSLKKASLNISNLTGANLSGA
                     NLSGANLSGANLFNADFSEADLSNANLTGANLTGAKFFKANLSRADLTNANLTRAILS
                     DADITRAIMTNANLNKAIMRNTNLSGSNLIRTDLSGANLLNANLFSADLHYANFSGAV
                     IHGANFTNADLSRATFVSSDITGANLSNSDLYFSDFSNANLTNVTMFRSAVSKAIFFN
                     TILTGATLANGKVFLFGVNDNPNIEYHE"
     gene            2761..4053
                     /locus_tag="PLB33_04015"
     CDS             2761..4053
                     /locus_tag="PLB33_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOG62721.1"
                     /translation="MKNKIKLFSNVIQHTANGGSLFYRYLQNNLNIMEKEIVNILENK
                     VLLINKRKDKIGKINLKDNLKKFYFIILILTLLFINGCASESGITSGVKRTPGGETVQ
                     LRETDNLTEAEKNKLRQNLIKEVREGFTFYRLSPGDMLEVMYHLSLVSEAQDYRIGVN
                     DELNVDFYFHPQVNRTVVVRPDGKITLPVKGDFQAAGLKPAELASNIKVQFSDIYNSP
                     VVTVSVNKFSSKMTELQKAITNAPRGQAKLVSIGPDGNVYLPLLRGIKAAGKTIDELR
                     DIINNEYRYEFNNLNVSILIESITGNRVFIFGEVPRPGPVQMTRPLTLLQLFANVGGY
                     LPTSAMDQVKIMYWNEKNEPILKTVNLYNIMNNLKLEEDMVVPNNSVIYVPKTGIAKL
                     NQWVDQYIRQLFLWQGENLGFSYGLGGKTLATGTISVE"
     gene            4126..6513
                     /locus_tag="PLB33_04020"
     CDS             4126..6513
                     /locus_tag="PLB33_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HOG62722.1"
                     /translation="MGAKKLLTLRDIFYIFFKNKTLIVSVFVTSIVIALVYCIVTPAQ
                     YRAETKILIKIGKAQFSGVELMPEKQNLLIQERSQNIRNEIELIKGQYLTEKVIERLK
                     GKIEPMKGDQSIFSRTFRNIKDMIKSVFTSVGITKKTPKDKGMILTFMSSLRVSFLED
                     TDMISLTFDWTDPKFAALVANLYADEYILQHTLVNESQRSHKFYNEQIELTDKRLKET
                     EDNLQMFLKDTNIANISLQKELHLRNLAELENKYNLAALDVSQAVAKIKKIKEMAAQP
                     NVWIETPEMGSTMVNRQEYLRTIDESYFRLKSERQRLLKIYTTEAAEIKSIDAQLDNL
                     RKQKADSLLNIANMELSLAENRKSSLAKDVENERKILNDVNSKTYLLRQLERDRELTE
                     LSYQMYKKKGEELRISDDLDSQRISSVRIATPAIPPLTPTYPKKGFIIIASAMIGLFL
                     SFGFSAIREFFNHTFRDDADIDEILGVPFLIAVPMRNVSGNNGVSSSNDSSTIANVLN
                     KILHRNGKKTATTTTSFITATPNGLAFIVLVIIGICGYFIYLYQSMMFFQNTIAMQRF
                     ISHDTQQTQQVMFASINPTDNVFLGRQNQESKNEVSSEDKNIGMDLLSDKMEQRRIEL
                     EKRRALLEVELEKIKSEIEISRMTKEQKNNHEAIQQADNQKAIQNTNVTPVQMQTMHQ
                     TNMSNVPTSTFIQQKSHNMRNNPPIDNAGQNHNKIIDNTSKKNEDKDQQEYVVGTNET
                     LVSILRDKYNIPTHLIYNESINLIRTANPGMKNLNSLAKGQKILIPKEVIEFAKK"
     gene            6564..7346
                     /locus_tag="PLB33_04025"
     CDS             6564..7346
                     /locus_tag="PLB33_04025"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOG62723.1"
                     /translation="MGRPRKIPIIEEKEIAEIDESLQLCNLRNMYDHFFKTLWKLKMD
                     LDEMKKGNEKEIVTKKRVGRKPKIKKETQISHSIVFSGFRRGDGASTMSLNFSYAFAE
                     NSANSVVLIDGNLRDPVLQNQFIMKRKKGLYDALQGNAGIQDIVNEIISKKFFFISAG
                     QQTKNPITLFESAKFEVLLKQLKEKYDLVIFDSAPLLGNPETTLLANKTDGLIMILNA
                     DVTRWEVAKSVKNDLEGANVRILGAILNRKQFIIPQAIYKFL"
     gene            7384..8856
                     /locus_tag="PLB33_04030"
     CDS             7384..8856
                     /locus_tag="PLB33_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOG62724.1"
                     /translation="MKTSNPSTETSVTKILFPIFMGVFAVVLAISIETLEYSTALAIT
                     AGLFVGGIAIYFLNQKAITYISIFTLGLGIPFNLDVNLFFRHYIGVTSVDIGVTFLSS
                     VVLYIVFYYDRIVSGIKRFYSNRMILFALVLYMLSGILSLYNALSKELVVLELVRLMF
                     LLIIFYTVMNFGKREYISVFILTLSICVFLEFLLSFYQYKTGRLFGLYAFGETRYEFG
                     LGYLQSRVSGTFSHANGLAYFFELLLPLMFAMILVEENKMLKLWYTVVFLFGLYGLII
                     TLSRGGWLATAVSLPIVFLLLFRKRITEKKFILSLFFGLAAVIVLGVIFYPTIAKRFQ
                     AYDYGSAGTRRPLNIAAISVIKQFPIAGVGMNNFAKVFRTYDTTGGSSLFQTQGLVHN
                     LFLGVWTETGTIGIIAFVWIFLSSFIVAIRLLSKVPLWYSGILVGVSAGLLAQIIHGM
                     FDPGFRVLMSTSMLVYSMIGLIGAVSVLYQKKKNNHQTKN"
     gene            8954..9898
                     /locus_tag="PLB33_04035"
     CDS             8954..9898
                     /locus_tag="PLB33_04035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62725.1"
                     /translation="MLPLSVIIVNWNSIELLKPCLVSLFEKNTSFDFEVIIVDNASKD
                     SSVEVLKKEFPLVHLIESDINMGFTRANNVAIRKAKGKYILLLNPDTIIIDVNILKKW
                     TDFMDSNPDVGASGCKLIYEDGRHQVGDAGYMPVINTVFNFSLFLSKLKPDRFKGIFL
                     NYNKLSKPIEVDWISGAGFLVRKEILETVGLMDEGIFMYADDIEWGCRIRKYGYRVVY
                     LPHMKIVHLQGGSAKKQKEISEFSFLWLKNIRLLFKYYNKNQPIVLYDLLISIGFLLR
                     AVLYFCLFIKTGKSDVKMKSHRMFKYFQFSISVFGKHA"
     gene            complement(9946..11106)
                     /locus_tag="PLB33_04040"
     CDS             complement(9946..11106)
                     /locus_tag="PLB33_04040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG62726.1"
                     /translation="MLAHNFYKYFAGEDSIALREKGLLEENKEQVYFYTKDNKETDSY
                     NLNKKINFFVQTIFSSSTKKELKTIIKDFKPDIAYTHNIFPLISPSIFHTLHAESIPC
                     VQNVQDYRWLCPNGVFYINDSICEKCKNGAFWNAIRYKCFRDSFLLSGLYATTIGTNR
                     LSGVFKKIDAFVCTTEFNKQKLMEAGIEEKRLFIKPNYLDITNIEPSVGTGNHIIFLG
                     RLSQEKGLWTLIKAFEKIKDLQLKIVGTGPLEHPLRKYIKENSINNISIEGFKEGDEK
                     NNLIKNALFMVFPSEWYEHFPIVLLEAFAFGKPVIASDIGNMPLIVENEKSGLHFKAG
                     DIDDLIEKIRILSQNNSEITRMGEFARKKIETYYTPEINYKILKSIFQKVAH"
     gene            11221..12180
                     /locus_tag="PLB33_04045"
     CDS             11221..12180
                     /locus_tag="PLB33_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOG62727.1"
                     /translation="MDSKIIDISVIIPTYNRASVLKKCLKALSKQSIPANTYEVIVSD
                     DGSEDDTKKVSEEALADTACFINYLWQPNKGANAARNNAIHAARGSILLFINDDTIAI
                     PSMLEEHLRTHKKYPEENISVLGRMTVSPEVPYSIFAKLHLDANYGLWKGMVELDWLA
                     FYTCNVSVKKSFLLKHGLFEESIRYHEDVELSERLSHFGFKIIYNPEALGYHDHYLQE
                     HEYLNVAKKDGIALAKWYKKSPHLKKQLSLVDFQLTVPFTKKMKYIIGDILINRATRP
                     AIIRTARLFSRSWEGLSLFLYRKIYQSIKRESVRQELMNSKID"
     gene            12191..13333
                     /locus_tag="PLB33_04050"
     CDS             12191..13333
                     /locus_tag="PLB33_04050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG62728.1"
                     /translation="MKPNITFLMSSAGTAYGGSETYILNVAKNLSDDFNVGLILGKGK
                     FTNDFNSLADENIIKYLSVPFISRNSKFSALLRKTKLHEKINDFDIEAITIMASTRKI
                     NKFISDSDILEVQYPTESLIFPFLQKNIKKIVHFHGTSPPSLYANIKNKINKYADAFI
                     TCSKWSKKLLEQTHNIENIKVIYNGVDANFFKPGNSEDFTLQQDYNHDLPRIGTVGRL
                     SSAKGTDLLFKVASELEGIAEFFAVGPYEENFIKNNLKKATSNFHFLGPLPNNALPPF
                     YKFIDCFVLPSFEETFPVTVLEAMSCGKPVIASNVGGIPEAIDDGYNGILCETGNYVL
                     LKELILKVINEENLRINISKAARQKVLEKFTIQKTSEELVQFYKNL"
     gene            13470..14618
                     /locus_tag="PLB33_04055"
     CDS             13470..14618
                     /locus_tag="PLB33_04055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOG62729.1"
                     /translation="MKPNITFLMSSAGTAYGGSETYILNVAKNLSDDFNVGLILGKGK
                     FTNDFNSLADENIIKYLSVPFISRNSKFSALLRKTKLHEKINDFDIEAITIMASTRKI
                     NKFISDSDILEVQYPTESLIFPFLQKNIKKIVHFHGTSPPSLYANIKNKINKYADAFI
                     TCSKWSKKLLEQTHNIENIKVIYNGVDANFFKPGNSEDFTLQQDYNHDLPRIGTVGRL
                     SSAKGTDLLFKVASELEGIAEFFAVGPCEEDLIQQIKRSNISNFHLLGPLPNNALPPF
                     YNFIDCFVLPSLFEAFGITLIEAMSCGKPVIASDVGGIPEIVDDGCNGILVDPGNYML
                     LKEAIIRIIYEKNLRIDLGESARQKIVKNFTFKKTCRELRDFYSSLIN"
     gene            14634..15824
                     /locus_tag="PLB33_04060"
     CDS             14634..15824
                     /locus_tag="PLB33_04060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOG62730.1"
                     /translation="MLAIEGGKPIRKKENFLVFGAPLIEEEDVLEVVRCLRSRWIGTG
                     PRVHQFEEVFAEYKKTKYAVAVNSCTAALHLAMLASGVGHGDEVITTPMTFCATVNSI
                     IHCGAKPVLADCEKKTMNISPSEIEKKITSKTKAIIVIHFAGRCCNMESILDISKKYN
                     LMIIEDCAHAIESEYRGKKAGTFGDIGCFSFYVTKNITTGEGGMVVTDNETIASRIKT
                     LALHGMSKDAWKRFSDDGYKHYKVVYAGFKYNMTDMQASLGINQMKRIDAYWRRRKEI
                     WEFYNKQFSDLHCMLPADPEPDTKHGYHLYTPLIDVDRLGKSRDWFLDAFTSENIGVG
                     VHYVPIHLHPFYQTVYGWKIGDFPNAEFIGERTISLPLSPALSDEDVTDVVSVFRKVL
                     LKHK"
     gene            15821..16831
                     /locus_tag="PLB33_04065"
     CDS             15821..16831
                     /locus_tag="PLB33_04065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG62731.1"
                     /translation="MKIAIVSTVYKATPPQGYGGIERVVYFLAEELIRRGNEVVLFGL
                     PGSFCSGKTVEVDGYSPSNMPSGIKKKSDQISEEFLYLAMKEYLDSNPVDVIHDWSFH
                     NLYVLRHTDKFPFVVSTCVPNFPDYDRPNLVASSKAHAALYGRSTKYVRYGLNLDDYQ
                     YSYNKKDYFIHIAKIAKYKGQHIAALAFRNSKEKLQIAGNIEDHLYYLMVLKPLLCLL
                     QNVSYIGEIIGINDYLCGAKALIQTPRWFDAFPLVVLEAFASGTPVISFAEGGVPEQI
                     IHGFNGYLCMDKGDIITAVNNISQIKPQDCRTYAEENFSVRRMANDYMELYERVISGE
                     TW"
     gene            16852..18291
                     /locus_tag="PLB33_04070"
     CDS             16852..18291
                     /locus_tag="PLB33_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOG62732.1"
                     /translation="MKLFNYYLINVVAQGLGRVFTFGSNFIAFALIARFYGTDFFGQY
                     AYVLNYFGIFVILADFGLMPVLGKDIAQTKDSPRMYWGNFLILRLFLNVLVILGSIIT
                     AYYLRRDMFFVLIIGSLAIPFLASRFFEPIFQVFQRPWHSFYASIIYGVAHLIFLATA
                     FQFSNNLFSFIAAYISANVLYALVAFYLSGKSVRPLFNINKSIIQNIIKLAMPLGISS
                     IFIIISNRVCILMLAGMKSDYAVGIYSAAYKFVEIASFLAAMVTAPLIPIFAEKAKND
                     KASLRYITSDIAEFIAVILLPIGVICTSVSQEIISLVYGSKMLPSADVFNILIWVSVI
                     IFYSLLISSVVISIGVVRYAYWLGAVAAALSVGLNYLLIPKYSYIGSAWVALICEIVL
                     VGVTITYAIRHIGNFFRWKTWSQIIGLNLTMCMMINQNFIDLNILLKVVIVMVLYIFS
                     IIKLRLFRSDIFYVWSNKKIFSFINVFKK"
     gene            18332..19336
                     /locus_tag="PLB33_04075"
     CDS             18332..19336
                     /locus_tag="PLB33_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOG62733.1"
                     /translation="MKEYQRIIWIDNLKGIGVIFIIFGHSISCPDFLINYLFSFHVPL
                     FFFIAGLNFNERSLNNFRLFCIKRAKRLLVPYVFFNLISYVLWLSRVKLLNIPQEVPA
                     IKPLVGVLYGNGNGLWLIHNTPLWFFVCLFIAQIMLFMILRIAKSKSHLIVLLFVFAA
                     IGYLDSKYLNIRLPWSIDIAFTAVVFSGIGYIFKRYSHTYRTNNNIIILFVVLMLSLT
                     VVYFNGKIDMNYNKFNNIFLFYSGAFLGIFFWFNISVIVGHLSIFRYIGNNSLTIFAL
                     HIPALIIIVKLLMVMGIPYSVQSSNILFLFIHSILTILIIAPFNYILNRYIPFFAGTK
                     "
     gene            19434..20897
                     /locus_tag="PLB33_04080"
     CDS             19434..20897
                     /locus_tag="PLB33_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62734.1"
                     /translation="MFKINIRVNTFERLELFCSYGAVAFFIIYIIAGVIYLFYPNYLN
                     HIEPLVASIGIVMKNGEILYPMPVGVYSYNWSLYGPALFEVQAFFQWIGVISTIVTSK
                     LPGLFALIVTAIILLRISRNWLYRGYLLYLFPFGLMLFWNRAEPFLIMIVSISLLFGQ
                     NYVNKRYLPIIMGVLGGIASALKIHGAAYIFTAYLAVAASSCSISSILLFLVSSVLSF
                     SAFFLPDNVLFSGFWGYLSLVGIDHGLSLKLWIENLIYLAFLSFPFVVFWRGIKLKHT
                     MRLRITLILVVEFLITIVASKYGNGFYHLMPLIPMNAFIIQKLSEAVVVEKGLFKILY
                     VSLIVVSFIVVPLDFVLPMAKSWHQFNYAQKEIASLEKNFPGLMMGVTDEQGYPYAFM
                     RVMLRKRQIDYAAFMDLQVAGFKDDEFAERLERCKIPSILIPNYGEPFSLKNYYTSEP
                     LFSDKLREVFASKYILIQRGKYYSVYTCAFSSRDMLP"
BASE COUNT         7009 a         3067 c         3847 g         6999 t
ORIGIN      
        1 gaatggatgg tgatggtcca tcaaacggaa gagtaaataa ttttgggatt atgcttgcat
       61 gtaaaagctc atttgaactg gattatgtga ttgaaaaatt gatcggaact tctgaaccta
      121 tgccaataag cgacatggct ttgaaacata atttaataaa aaaatattct gcatgttgtg
      181 tttatccttt ttctaatgat attgatgttt gcaaaaaatg ttttgttgat tttaaatttc
      241 ctaaaaccat caatacggat tggagacttc cagtttttat aaaaaccaca ttaagcaaaa
      301 tttttataaa aaggccaaag ataaataaaa atagatgcag gaactgtcaa atatgcttaa
      361 atgtatgtcc tgtaaaagct atctcaaatt caggaataac tccaaatata aattataaaa
      421 aatgtattag ttgctactgt tgtcaggaaa tgtgcccaga aagcgctata acaatatcgt
      481 aattcgacaa tattatatca tgtatgatat caattagata taagataata ttaaataata
      541 tctttaaaat tgccattaat aaataattgt ttcatgaata aacaatctat taaatagctg
      601 atattacaaa tataattaat aacataaaat attaaccgat attataatga atatatttta
      661 attttatcgc gcaatcgata tattaaatat tacatagttg tgattatact aatataacta
      721 taatatcatg atgttacaat tggcatattt aatatgcagt tcaaatatat tatcagttat
      781 atatttgaat aataaaacat ttaataatat tattttcatt taataagctt taatacttct
      841 atatcaaata aggtgtttag gtaattccag catatataat aatattaaag atttatgtga
      901 taccagtaaa tgttattata attgtaaaat attctgttta ttcgaaatat ctgacagaca
      961 tattaaatat atataaaatt atggttgtga gatttcaaaa aataaacaat ctcattatta
     1021 taaatgcagt tatatagata taatacgaga aatataaaat aaataataaa ttactcgata
     1081 taagttaaat ttagaataaa aaataatata tattttaaat tagatagaat aatggttcat
     1141 gatattcaac gtacataaaa agcaataatt agttatacat taaatcaaat ataaacaacg
     1201 ataaaatata ataaaattat caatcgtcta aaattatcaa aacgaaataa ataatattgt
     1261 taatgataac atatgataca atattaatta agaggtatta aaaattaaat attcattaaa
     1321 gaaaaatgac agataatata tttagtatta ggtaatatta aagatataat tagataacct
     1381 tatattttga aaaaagagtt tatacaaaca atgctaataa aagctttatt atcaataggt
     1441 aacaaagtat ttaaatatat atttagttta aaatatatat ttaaataaat ttgaatatta
     1501 aaataaaatc ttattgtata tttagaatta tacagattaa taattaatct ttattcatga
     1561 tattcgatgt ttggattatc atttacaccg aaaagaaaaa ctttaccatt tgctaaagtg
     1621 gcgccagtta aaattgtatt gaagaatatg gcttttgaga cagcagacct gaacattgtt
     1681 acattagtta aatttgcatt tgaaaaatca gaaaaataaa gatctgagtt actaaggtta
     1741 gctcctgtaa tatcagaact tacaaaagtt gctctgctta aatctgcatt tgtaaaatta
     1801 gctccatgta taacagctcc agagaagttt gcataatgca aatctgcact gaagagatta
     1861 gcattcaaaa gattagctcc agataaatca gtccttatca ggtttgatcc tgatagattg
     1921 gtgttcctca ttatagcttt atttaaattg gcatttgtca taatggcacg cgtgatatca
     1981 gcatcagaca aaatagccct cgtcagattg gcatttgtaa ggtcggccct tgaaagatta
     2041 gctttaaaaa attttgcacc ggttagattt gcaccggtta gatttgcatt ggaaagatct
     2101 gcctctgaaa aatcagcatt aaataaattt gctcctgata aatttgctcc tgataaattt
     2161 gctcctgata aatttgctcc tgtgaggttt gaaatattta gggaggcctt tttcaagctt
     2221 gaaaagtaaa aatcggtatt tgtcaaatca gtatctgtca tttttgcatt atcaagaatc
     2281 gatgctgtca agtccttgtg tgttaggtct aatctgaata aatcgcagtt ctgacatgtg
     2341 tttgttgtga tcaattttat ataatcatcc tggttaaatg cgaaagtgtc gaacttatca
     2401 aataacatgt taacaaaaac tgttgtaaat attacaaaat acagaaattt aaatttattt
     2461 atcataattg ttcctccaat atttatttta attcaaaaat atgacgaagc atacttaaaa
     2521 taaaaaatat aaaataataa ttcactgtct aaaaatatat aaatttttaa attcatctta
     2581 tataattcta aaagcaataa tatcagcatt ctataaatta tgataaatag aacatatgta
     2641 tttaattttc gcacaatttt aaaatatgtc aataaaaaaa gaatgttgta taaataattt
     2701 tcttgacatt ttttaatggc gttgtacaat taatattatt attgtaataa aagatatttt
     2761 atgaaaaata aaataaagtt attttcgaat gttattcaac atactgccaa cggcggtagc
     2821 ctattttaca ggtatttaca aaataattta aatattatgg aaaaagaaat agttaatatt
     2881 ttagaaaata aggttcttct aattaataaa agaaaagata aaattggaaa gataaattta
     2941 aaagataatt taaaaaagtt ttattttatt attttaattt taacattact atttattaac
     3001 ggatgtgctt ctgaatctgg cattacaagc ggagtgaaaa gaacgccagg aggagaaact
     3061 gttcagttaa gagaaacaga taatttaacc gaagctgaaa aaaataaact acgacaaaat
     3121 cttattaaag aggttagaga aggctttact ttttacagac tatcgcctgg tgatatgctt
     3181 gaagtaatgt atcatctgtc acttgtctca gaggcgcagg attacagaat aggtgttaat
     3241 gatgaattaa atgttgattt ttattttcat ccacaagtta acagaactgt cgtagttcgt
     3301 cctgatggga aaattacatt acctgttaaa ggcgattttc aagctgctgg actaaagcct
     3361 gcagaacttg catctaatat aaaggttcaa ttttcagaca tatataattc gcctgttgtt
     3421 accgttagcg taaacaagtt ttcttcaaaa atgacagaac ttcaaaaggc aataacaaat
     3481 gctccaagag gccaagcaaa attggtttcg attggaccag acggtaatgt ctatcttcct
     3541 cttttaagag gtattaaagc tgccggtaaa acaatagatg aattaaggga cataattaat
     3601 aatgaatata gatacgagtt caataatcta aatgtttcta tattaataga gagtattact
     3661 ggaaatcgtg tttttatttt tggcgaggtg ccaagacctg gtcccgtaca aatgacaagg
     3721 cctctaactt tactgcagct ttttgcaaac gtaggggggt atcttccgac cagtgctatg
     3781 gaccaggtaa agataatgta ctggaatgaa aaaaatgaac ctattttaaa gactgttaac
     3841 ctttataata ttatgaataa tctgaaacta gaagaggata tggtcgttcc taataatagt
     3901 gtaatttatg taccgaaaac aggcatagca aagcttaacc agtgggttga tcaatatata
     3961 agacagttat ttttgtggca gggcgagaat ttaggattct cgtatggatt aggtggaaag
     4021 actttggcta caggtacaat ttcggttgaa taggattgga gtaataagaa atatgtttca
     4081 agcttgcatt tatataaaag aaaaacaata caacaggaac ggaagatggg agcaaagaag
     4141 cttttaacat taagagacat attttatata ttcttcaaga ataagacact aattgtttct
     4201 gtgtttgtta cttctatcgt tattgcattg gtttattgta ttgtgactcc tgcgcaatat
     4261 cgtgcagaaa caaagatatt gatcaaaatt ggtaaagcac aattttcagg ggtcgagcta
     4321 atgccagaaa agcaaaattt gcttatccaa gaaagaagcc agaatatccg caacgaaatt
     4381 gaattgataa aaggtcaata tttaacagaa aaggtaatag aaagacttaa aggtaagatt
     4441 gaacctatga aaggggatca atctatattt tcgaggacat tcagaaatat aaaggatatg
     4501 atcaagtctg ttttcacctc agttggaata acaaaaaaga ctcccaaaga taagggcatg
     4561 atattaacgt ttatgagttc tttaagggtt agttttcttg aggacacaga tatgatttct
     4621 ttaacgtttg attggaccga cccgaaattt gcggcgttgg ttgcaaattt atatgcagat
     4681 gaatacatat tgcaacatac actcgtaaat gaatcacaga ggtcccataa gttttataat
     4741 gagcagattg agttgaccga taagaggctt aaggaaaccg aagataatct ccagatgttt
     4801 ctgaaagata caaacatagc aaatatatct ttacaaaagg aattgcatct gagaaacctt
     4861 gcagaactcg aaaataaata taaccttgca gcgcttgatg tttcgcaagc cgtggcaaaa
     4921 ataaaaaaga ttaaggaaat ggctgcgcag ccaaatgtct ggattgaaac acccgagatg
     4981 ggaagcacta tggtgaatag acaggaatat ttgcgaacaa tcgatgagtc atattttaga
     5041 ttgaaaagcg aacgtcaaag acttctaaaa atatatacaa cagaggcggc ggaaattaag
     5101 tctatagatg cacagcttga taatcttcga aaacaaaagg ctgatagcct tctaaacatc
     5161 gcaaatatgg aattgtcatt ggctgagaat agaaaaagta gtttagcaaa ggatgtagag
     5221 aacgaacgaa agatattgaa tgatgtaaac tcaaaaacat atttattaag acagcttgaa
     5281 agggatagag aattaactga acttagctat cagatgtata agaaaaaggg cgaagagcta
     5341 agaatttctg atgaccttga ttctcagaga atttcaagtg taagaattgc tacacctgct
     5401 attccgcctt tgacaccgac ttatccaaag aaaggtttta ttataattgc gtctgcgatg
     5461 ataggtctct tcctcagctt tggtttttct gctatacgtg aattttttaa tcataccttc
     5521 agggatgatg cagacattga tgagatcctt ggtgtcccat tcttgattgc agtcccgatg
     5581 agaaatgttt caggaaacaa tggtgtatct tcctcgaatg attcaagtac tattgcaaat
     5641 gttttaaaca aaatattgca tcgtaatgga aagaaaacag ctacaactac aacaagtttt
     5701 ataacggcta caccaaacgg attagccttt attgttttag ttattattgg aatatgtgga
     5761 tattttatat acctatatca aagcatgatg ttttttcaaa acaccatagc gatgcaacgc
     5821 tttatttcgc acgatacgca acaaacgcaa caggttatgt ttgcctcaat caacccaact
     5881 gataatgttt ttttaggcag acaaaatcaa gaaagtaaaa acgaagtttc ttctgaagat
     5941 aaaaatatag gcatggatct gttaagtgac aaaatggagc aaaggcgaat tgaattggaa
     6001 aaacgtagag ctttgcttga ggttgaactc gaaaagatta aatcagaaat tgaaataagc
     6061 aggatgacga aagaacaaaa aaataatcat gaagccattc agcaagcgga taatcagaag
     6121 gcaattcaaa atacgaatgt aaccccagta caaatgcaaa caatgcatca aacgaacatg
     6181 agcaatgttc caacgagtac ctttattcaa caaaagtctc ataatatgcg aaataaccct
     6241 cctattgata atgcaggaca aaatcacaac aaaataatag ataatacatc aaagaaaaat
     6301 gaagataagg accaacagga gtatgtagtg ggaactaacg aaacattagt gagtattcta
     6361 agggataaat acaatatacc tacacattta atctataatg aatcaataaa tctgataagg
     6421 actgctaatc ctggaatgaa aaatttaaat agtcttgcaa agggtcagaa aatattaata
     6481 ccgaaagaag taatcgaatt tgcgaaaaaa taattgtaca agtcatcttc attttgaatt
     6541 atttattaaa aatggaattt taaatgggta gacctcgaaa gataccgata attgaagaaa
     6601 aagaaattgc cgaaattgac gagagtcttc aactctgcaa tcttagaaat atgtatgacc
     6661 atttttttaa gacactttgg aaattgaaaa tggatcttga tgaaatgaaa aagggaaacg
     6721 aaaaggagat tgtaacaaaa aaaagagttg gaagaaaacc aaaaataaaa aaagaaacgc
     6781 agatcagtca ttcaatagtt ttttctggtt ttagacgtgg agacggagct tcaacgatgt
     6841 cattgaattt ttcatatgcc tttgctgaaa attctgcaaa tagtgtcgtt ctcatagatg
     6901 gaaatttaag agaccctgtt cttcaaaacc aatttataat gaaaagaaaa aaaggtctat
     6961 atgatgcctt gcagggcaat gctggtatac aggatattgt gaatgagatc atttcaaaaa
     7021 agtttttttt tatttctgct ggtcaacaga caaaaaatcc tataacatta tttgaatcag
     7081 ccaaattcga agttctctta aagcaattaa aagaaaaata cgacctcgta atatttgatt
     7141 ctgctccctt attggggaat ccagaaacta cattattggc gaacaaaaca gatggtttga
     7201 ttatgatttt aaatgccgat gttacaagat gggaagtggc gaaatcagtg aaaaacgatc
     7261 tggaaggtgc aaatgtcaga atattggggg ctattcttaa cagaaagcag tttataatac
     7321 cacaggcaat atacaaattc ctctaattgc gatataataa ttacctataa aacttaatgt
     7381 gttatgaaaa catcaaatcc atcaactgaa acatcagtaa caaaaatatt atttccaatt
     7441 tttatgggtg tttttgcagt tgtattagca atatcaatcg aaacacttga atacagcact
     7501 gcgcttgcca ttacagcagg attatttgtt ggcggtatag caatatattt tttgaatcag
     7561 aaggccataa cgtacatatc aatttttacg cttggtcttg gaataccttt caacctcgat
     7621 gttaacctgt tttttagaca ctacattggt gtaacaagtg ttgatattgg tgtaacattt
     7681 ctttctagcg tagtcttgta tattgtattc tattatgacc gtatcgttag tggaataaag
     7741 cgtttttaca gcaacagaat gattttattt gcattagttc tctatatgtt aagcggtata
     7801 ctgagcctgt ataatgcatt gtctaaagaa ctcgtagtgc ttgagcttgt tcgtttaatg
     7861 tttcttttga taatatttta cactgtaatg aattttggaa agagagaata tatatctgtt
     7921 tttattttaa cgttgtccat atgcgtcttt ttggaatttt tactttcatt ttatcagtat
     7981 aaaacaggcc gtctttttgg tttatatgcc tttggggaaa caagatatga atttggactt
     8041 gggtacttac aaagcagggt atcgggtacg ttcagccatg caaatggact tgcgtatttt
     8101 ttcgaactgt tattgccttt gatgtttgct atgatcctgg ttgaagagaa caaaatgctt
     8161 aaattatggt acactgttgt cttccttttt ggtctttatg ggttgattat aacactttca
     8221 agaggtggtt ggttagccac tgctgtttct ctaccgattg tatttttatt gttgtttagg
     8281 aaaaggatta cagagaaaaa attcattttg tcactctttt ttggattagc cgctgtaatt
     8341 gttttgggtg ttatttttta tcccacaatc gcaaaaaggt ttcaggctta tgactatggt
     8401 tcggctggaa cgagacgtcc attgaacatc gctgcaatat cggtaataaa gcaattccct
     8461 attgcaggcg taggcatgaa taattttgcc aaggttttca ggacgtatga tacaacaggg
     8521 ggttcatctt tatttcagac gcagggtctt gttcataatc tttttcttgg agtgtggact
     8581 gaaaccggta caatcggaat cattgctttt gtatggattt tcttgtctag ttttattgtg
     8641 gctatcaggt tattatcaaa agtaccgctt tggtattcag gaatattggt tggcgtttct
     8701 gccggccttt tagcgcagat tattcatggg atgtttgacc ctggttttcg tgttttaatg
     8761 agcacatcaa tgcttgtata cagtatgatt ggcttaatag gagcggtaag tgttttgtat
     8821 caaaaaaaga aaaacaacca tcaaacaaag aactgaattc ttgaaaatca ccctgtaaag
     8881 tagtgttgat tgatgataaa atgctttgca gggttttttt tgcaatgaaa aaggtctata
     8941 ctattttatg aggatgctgc cgctatcggt tattattgta aattggaatt caattgaact
     9001 gcttaaacca tgccttgttt ctctttttga aaaaaacaca agttttgatt tcgaagtcat
     9061 aatagtcgat aatgcttcca aagatagctc tgtagaagtt ctaaaaaaag aatttccact
     9121 tgtacatttg atcgagagcg atatcaatat gggttttacc agggcaaata atgtggcaat
     9181 aagaaaggcc aagggaaaat atattctgct tttaaacccc gacactatca taattgatgt
     9241 caatatttta aaaaaatgga ctgattttat ggatagcaac cctgatgttg gagcaagtgg
     9301 ttgcaagttg atctatgaag atggccgcca tcaggttgga gatgctggtt atatgcctgt
     9361 aattaatact gtatttaatt tctcattgtt tctgtctaaa ttgaaaccag atcgattcaa
     9421 aggcattttt ttaaattaca ataaactaag taagccaatc gaggttgact ggataagtgg
     9481 cgctggattt ttagtaagaa aagaaatcct tgaaacagtt ggtttgatgg atgaaggcat
     9541 ttttatgtat gccgatgata tagaatgggg ttgtcgtatc agaaaatatg gatatagggt
     9601 agtgtatttg ccccatatga aaatagtcca tttgcaaggc ggcagtgcaa aaaagcaaaa
     9661 agagataagc gaattttctt ttttgtggct taaaaatatc cgtttactct ttaaatatta
     9721 caacaagaat caaccaatcg ttttatatga tttattgatc tcaattggat ttttgctaag
     9781 agctgttttg tatttttgtt tgttcatcaa aacaggaaaa agtgatgtaa agatgaaatc
     9841 ccataggatg tttaaatatt ttcaattcag catatctgtt tttggaaaac atgcgtgatt
     9901 agcttatagt tagtatgagt gttgcctggt tgtattgtgt acaatttagt gggctacctt
     9961 ttgaaagatt gatttcaaga ttttgtagtt tatctctggg gtgtaataag tttcaatctt
    10021 tttacgtgca aactctccca tccttgtgat ttcagaattg ttttgagaga gaattctaat
    10081 tttttctatc aaatcgtcaa tatctccagc cttgaaatgt aatccacttt tttcattttc
    10141 aacaatcagg ggcatattgc ctatatcaga tgcaatgact ggtttgccaa aggcaaatgc
    10201 ctcaagaaga acaatcggaa aatgctcgta ccattctgaa gggaaaacca taaataaagc
    10261 attctttata agattgtttt tttcatcacc ttccttgaat ccctcaattg atatgttgtt
    10321 tatagaattt tcttttatat attttcttaa tgggtgttct aatggacctg tgccaacaat
    10381 ctttaattgg aggtctttaa ttttttcgaa ggcctttatg agtgtccaca ggcctttttc
    10441 ctgggataac ctacctaaga atataatatg atttcctgtg ccaaccgatg gttcaatatt
    10501 agttatgtct aaataatttg gttttataaa aagccttttt tcttcaatgc cagcctccat
    10561 cagtttttgc ttgttgaact ccgttgtgca tacaaacgca tcaatctttt tgaagacgcc
    10621 agacaacctg ttagttccta tcgtggtagc atataatcca ctcaacagaa agctatctct
    10681 gaaacattta taacgtatcg cattccagaa tgcaccattt ttacattttt cacatatgct
    10741 gtcatttatg taaaatacac cgttagggca gagccacctg taatcctgaa cgttctggac
    10801 gcaggggatt gattcggcat ggagagtatg aaaaatggat ggtgaaataa ggggaaatat
    10861 attgtgcgta taggctatat cgggtttaaa atcttttata atggttttca gttctttttt
    10921 tgttgaagaa gaaaaaatgg tttgtacaaa aaaatttatt tttttgttta aattatagct
    10981 atcagtttct ttattgtcct ttgtgtaaaa atatacctgt tctttgtttt cttcgagcag
    11041 tcccttttcc cgtaaggcga ttgagtcctc accggcaaaa tatttataaa aattatgtgc
    11101 aagcaaaata ttcattggta tacaaaaaat atttatatga tagcaaataa atgtgattta
    11161 agctatctta atacagaata gatatttttg aaagattatt gttatgaaag ggtttatctg
    11221 atggattcta aaataattga tatttcggtc attatcccga catacaatcg tgcatctgta
    11281 cttaaaaaat gtcttaaggc gctttcaaaa caaagcattc ctgcaaatac gtatgaggtt
    11341 attgtatctg acgatgggtc tgaagatgat acgaagaagg taagcgaaga ggcattagca
    11401 gatacagcat gttttataaa ttatttatgg caacccaata aaggtgctaa tgcagcaagg
    11461 aacaacgcaa ttcatgctgc aaggggtagc attttattat ttataaacga tgatactatt
    11521 gcaatcccgt ctatgttgga agagcattta cggacacata aaaaataccc tgaggagaat
    11581 atttcagtat tgggcaggat gacagtgtcc cctgaggtgc catattcgat ttttgctaaa
    11641 ctgcatcttg atgccaatta tggattgtgg aaaggaatgg ttgaactgga ttggcttgca
    11701 ttttacacat gtaatgtttc cgtcaaaaaa tcatttttat taaaacatgg tctttttgaa
    11761 gaaagcatta gatatcacga agatgtggag ctttcggagc gtttatccca ttttggtttt
    11821 aaaattatat ataatccaga ggcattaggt tatcacgacc actatcttca ggaacatgaa
    11881 tatctgaatg tagcaaaaaa agatggtata gcattagcga aatggtataa aaaatcaccc
    11941 cacctaaaaa aacaactcag tctcgttgat tttcagctaa cagtgccttt cacaaaaaaa
    12001 atgaaatata ttataggtga catattaata aatagggcaa caagaccagc aataattaga
    12061 acggcaaggc ttttttcaag gtcatgggag ggcttatctc tatttcttta cagaaagata
    12121 taccaatcca ttaagaggga aagcgttcga caggaactga tgaattcaaa aatagattga
    12181 atatttcaac atgaagccaa atataacttt tttgatgtct tctgcgggta cagcttacgg
    12241 tggatcagaa acatacattt taaatgttgc aaaaaacctt tctgatgatt tcaatgtagg
    12301 gcttattctt gggaaaggta aatttacaaa tgatttcaac tcattagctg atgaaaacat
    12361 cattaaatac ctaagcgttc catttatctc aagaaactct aaattttcag cccttctcag
    12421 aaagacaaag ctacatgaaa agataaatga ttttgatatt gaagcaataa ctataatggc
    12481 ttcaacaaga aagattaaca agtttatatc agattctgac atccttgaag tccagtatcc
    12541 aacagaaagc ctcattttcc cgtttcttca aaagaatatt aaaaaaatag ttcattttca
    12601 tggaacctcg ccaccctctt tgtatgctaa tatcaaaaat aagattaata aatacgctga
    12661 tgcatttatt acatgtagta aatggagcaa aaaattatta gagcagacac ataacattga
    12721 aaatatcaaa gtaatttata atggtgttga cgcgaatttt ttcaagcctg gaaattcaga
    12781 ggattttact ttacagcagg attataatca tgatttgccg agaataggta ctgttggtag
    12841 attgagcagt gccaaaggga cggatttgct ttttaaggtt gcatcagaac tggaaggcat
    12901 tgctgagttt tttgcggtcg gtccttacga agaaaatttt attaaaaata atttaaaaaa
    12961 ggctaccagc aattttcact ttcttggccc gttacccaat aatgctttgc ctccttttta
    13021 taaatttata gattgttttg tattgccatc atttgaagaa acttttcctg taacggtgct
    13081 tgaagctatg tcatgcggta aacctgttat agcatcaaac gtaggtggca tccctgaagc
    13141 tattgatgat ggatataatg gtattttatg cgagacagga aattatgttt tattgaaaga
    13201 gttgatactt aaagtaatta atgaggaaaa tctgaggatt aatatatcta aagcagccag
    13261 acagaaggta cttgaaaaat ttactatcca aaaaacatct gaagaattag tgcagtttta
    13321 caagaacttg taatgattta tttatcgaat aaatataaga tgctacataa tattaacctt
    13381 ttcctcgata ttaaagatat accaatccat taagagggaa agcgttcgac aggaactgat
    13441 gaattcaaaa atagattgaa tatttcaaca tgaagccaaa tataactttt ttgatgtctt
    13501 ctgcgggtac agcttacggt ggatcagaaa catacatttt aaatgttgca aaaaaccttt
    13561 ctgatgattt caatgtaggg cttattcttg ggaaaggtaa atttacaaat gatttcaact
    13621 cattagctga tgaaaacatc attaaatacc taagcgttcc atttatctca agaaactcta
    13681 aattttcagc ccttctcaga aagacaaagc tacatgaaaa gataaatgat tttgatattg
    13741 aagcaataac tataatggct tcaacaagaa agattaacaa gtttatatca gattctgaca
    13801 tccttgaagt ccagtatcca acagaaagcc tcattttccc gtttcttcaa aagaatatta
    13861 aaaaaatagt tcattttcat ggaacctcgc caccctcttt gtatgctaat atcaaaaata
    13921 agattaataa atacgctgat gcatttatta catgtagtaa atggagcaaa aaattattag
    13981 agcagacaca taacattgaa aatatcaaag taatttataa tggtgttgac gcgaattttt
    14041 tcaagcctgg aaattcagag gattttactt tacagcagga ttataatcat gatttgccga
    14101 gaataggtac tgttggtaga ttgagcagtg ccaaagggac ggatttgctt tttaaggttg
    14161 catcagaact ggaaggcatt gctgagtttt ttgcggtcgg tccttgtgag gaagatctta
    14221 ttcaacaaat aaaaaggagt aatattagca actttcattt gttaggccct ttaccaaaca
    14281 atgctttacc tcctttttat aattttatag attgttttgt attgccatct ctatttgagg
    14341 cttttggaat cacattgatc gaggcaatgt catgtggtaa gcctgtcata gcatcagatg
    14401 tggggggtat tcctgaaatt gttgacgacg gttgcaatgg aattttggta gatcctggaa
    14461 attatatgtt attaaaagaa gcgataatac ggataatata tgaaaaaaac ttgagaattg
    14521 atttaggtga atctgcgaga cagaaaatcg ttaaaaattt tacatttaaa aaaacatgca
    14581 gggagttaag agacttttat tcgagtttaa taaactgagg agacaaaaag taaatgcttg
    14641 caatagaagg gggaaaacct ataaggaaaa aagaaaattt tttggtgttt ggtgcacccc
    14701 taattgaaga ggaagatgta cttgaggttg taagatgctt aagaagcaga tggataggaa
    14761 cgggccccag ggtccatcag ttcgaagagg tttttgcaga atataaaaaa acgaaatatg
    14821 cagttgctgt aaattcatgt accgctgccc ttcatcttgc catgcttgca agcggggtag
    14881 ggcatgggga tgaggttata accacgccca tgactttctg tgcgaccgtc aattcaataa
    14941 tacactgcgg tgcaaaacct gttcttgcag attgcgaaaa gaaaacaatg aacatatcgc
    15001 catctgagat agaaaaaaag ataacatcaa aaacaaaggc aataattgta atacattttg
    15061 ccggtcgttg ctgtaacatg gagtcaattc tggatatctc aaaaaaatat aatttaatga
    15121 ttatagagga ttgtgcgcat gcaatagagt cagagtatag aggaaaaaag gcaggaacct
    15181 ttggtgatat tggttgtttt agtttttatg ttactaaaaa cattacaaca ggggaaggtg
    15241 gtatggtagt cacagacaac gaaacaatag catcaaggat taaaacactt gccctgcatg
    15301 gtatgagcaa agatgcatgg aaacgttttt ccgatgacgg atacaaacat tataaggttg
    15361 tttatgctgg ttttaaatac aatatgacag atatgcaggc atcgcttggg ataaaccaaa
    15421 tgaagcgcat tgatgcttat tggagaagac gaaaagagat atgggagttt tacaacaaac
    15481 aattttcaga tttacattgt atgctgcctg ctgacccgga acccgataca aagcatggtt
    15541 accatctata tactccgtta attgatgttg atagacttgg aaaaagcagg gattggtttc
    15601 tggatgcatt tacctctgaa aatatcggtg taggggttca ctatgtcccc atacacctac
    15661 atccttttta tcaaactgtg tatggctgga aaataggtga ttttccaaat gctgagttta
    15721 taggagaacg taccatttcc cttccattgt ctccagcatt gagcgatgaa gatgtaactg
    15781 atgttgtaag cgtattcaga aaggttctat taaaacataa atgaagattg caattgtatc
    15841 aactgtatat aaagcaacac ctccacaggg gtatgggggt attgaacgtg ttgtatattt
    15901 tttggctgag gaacttataa gacggggcaa tgaagtggta ctatttgggt tgccaggtag
    15961 tttttgttcg ggcaaaacag ttgaagtaga tggttatagc ccctccaata tgccaagcgg
    16021 aattaagaaa aaatcggatc aaatatcaga ggagtttctt tatctggcta tgaaggaata
    16081 tcttgatagt aatcctgtag atgttataca cgattggtct tttcataacc tttatgtgtt
    16141 aaggcataca gacaagtttc cttttgtggt atcaacctgt gttccaaatt ttcccgatta
    16201 tgatagaccc aatcttgtgg cgtccagtaa agcccacgca gcgctttatg gcagatcaac
    16261 aaaatatgtg cgctatgggc taaatctcga tgattatcag tacagttaca acaagaagga
    16321 ttattttata catatagcga agatagctaa atacaaaggc caacatatag ctgctctggc
    16381 attcagaaac tcaaaggaaa aacttcaaat agcgggtaac atcgaagacc atttatatta
    16441 tttgatggtt ttaaaaccac tgctttgtct tttgcaaaac gtttcatata tcggagagat
    16501 tataggaata aacgactacc tctgcggggc aaaagccctt attcagactc cacgatggtt
    16561 tgatgcattt cctcttgtcg ttttggaggc ttttgcatca ggaacccctg taatttcttt
    16621 tgctgagggt ggggtaccag agcagataat tcatggtttt aatggatatc tctgtatgga
    16681 taaaggcgac ataattacag cggttaataa tatatcgcag attaaacctc aagactgtcg
    16741 tacgtatgct gaagaaaatt tctcagtaag aaggatggca aatgattata tggaattgta
    16801 tgaaagggtg ataagcggtg aaacttggta gacaaaacat aatggaaaac catgaaattg
    16861 tttaattatt acctgatcaa cgttgttgct cagggtttag gaagagtttt tacgtttggt
    16921 tcaaatttta tagcctttgc tcttattgca cgtttttacg gtacagattt tttcggacag
    16981 tacgcatatg tattaaatta ttttgggata tttgtaattt tagcagactt tggtttaatg
    17041 cctgtgctcg gcaaagatat agcccaaacc aaagactctc ctcgcatgta ttggggaaat
    17101 tttctaattt taagactctt tctcaatgtc ttggtgatct tggggtctat aatcacagct
    17161 tattatttac gtagagacat gttctttgtt ctaattattg gctctttagc aatacctttt
    17221 ttggcgtcga ggttctttga acctatattt caggtttttc aacgtccctg gcattctttt
    17281 tatgcctcaa taatatatgg ggttgcccat cttatttttc ttgccacggc atttcagttc
    17341 tcgaataacc tgtttagttt tatcgccgcc tatatatctg caaatgtttt atatgctctt
    17401 gtagcattct acttatcagg gaaatccgta aggcctttgt ttaacatcaa taaatcgata
    17461 atacaaaaca taatcaaact tgctatgccc ttgggcattt cctcaatatt tataataatc
    17521 agcaaccgtg tttgtattct gatgcttgct ggaatgaaat ctgattatgc agttggaata
    17581 tacagcgctg catacaaatt tgttgaaata gcatcttttt tggcggctat ggtaactgcg
    17641 cctttgattc ctatatttgc ggaaaaggct aaaaatgaca aggcatcact gagatatata
    17701 acgagcgaca tagctgaatt tattgccgtt attcttttac caataggtgt aatatgtact
    17761 tcagtgtcgc aagagattat ttccctagtt tatggtagca agatgctgcc gtctgcagat
    17821 gtatttaata tattgatttg ggttagtgtc attattttct attcattatt gatatcaagc
    17881 gttgttatct caattggtgt tgtaagatat gcatattggc tcggtgctgt tgctgctgct
    17941 ttaagcgttg gtttgaatta tttattgata ccaaaataca gctatattgg ttctgcatgg
    18001 gtggcattga tatgtgagat tgtgttggtc ggggtcacga tcacttatgc aattcgtcat
    18061 attgggaatt tcttcagatg gaaaacatgg agtcagatta ttggcctaaa tcttactatg
    18121 tgtatgatga taaatcaaaa ttttattgat ctgaatattt tacttaaggt tgttatcgtt
    18181 atggttttat atatcttctc tataataaaa ttgcgcttgt ttagaagcga catcttctat
    18241 gtatggtcaa ataaaaaaat attttccttt ataaatgttt ttaagaagta gccgcacaat
    18301 aggattaaac tcaagggcag acttagttta aatgaaagaa tatcaaagga ttatttggat
    18361 agacaacctg aagggcatcg gggtcatttt tattattttt ggtcactcaa tctcatgtcc
    18421 agattttctt atcaattatc ttttttcatt tcatgtaccc ctatttttct tcatagccgg
    18481 attgaacttc aatgaaagat cgttgaacaa ttttagattg ttttgcataa aaagggcaaa
    18541 acgactattg gtgccttatg ttttttttaa tctaatctct tatgtgctat ggctttcaag
    18601 ggttaaattg ttgaacattc ctcaggaagt accagcaatt aagccgttgg ttggtgtttt
    18661 gtatggcaat ggaaatggtt tgtggctgat tcataacacg ccactgtggt tttttgtatg
    18721 tttatttata gcccagatta tgctttttat gattctaagg attgccaaat cgaaatcgca
    18781 tcttattgtg cttttgtttg tgtttgccgc gatcggatat ttggattcaa aatatttaaa
    18841 tataaggctt ccttggagta tcgatatagc gtttactgct gtagtttttt ctggtattgg
    18901 ttatattttt aaacgatata gccataccta cagaacgaat aataatatca taattttgtt
    18961 tgttgttttg atgctgagtt taactgtagt ctacttcaat ggaaagattg atatgaacta
    19021 taacaaattc aataatatct ttttgtttta tagtggtgca tttttgggta tcttcttttg
    19081 gtttaatatt tcggtaatag ttgggcactt aagtatattt agatatattg ggaacaactc
    19141 tttgacgatc tttgctctac atattccggc attaattatt attgttaaat tattgatggt
    19201 tatgggtatt ccatactctg tacaatcttc aaacattctg tttttgttta ttcattctat
    19261 tttaaccatt ttgattatag cgcccttcaa ttatatatta aacagataca ttcctttttt
    19321 tgctggaacg aaataatatg gaatatatag cagatccgtt aaagtttatt ttacacaatg
    19381 ccacaatgat tggaggaacc gtattttgtt tcattgtatt gatggttttt catttgttta
    19441 aaataaacat tagagtaaat acttttgaaa ggcttgagct tttttgtagt tatggtgcag
    19501 tagctttttt tataatttat ataatagcag gtgttattta tcttttttat cccaattatc
    19561 ttaaccatat tgaacctttg gttgcctcca ttggcatagt tatgaagaac ggagagattc
    19621 tatatcctat gcctgtcggt gtttactcct ataactggag cctttacggt ccagctttgt
    19681 ttgaggtaca agcttttttt caatggattg gtgtaatatc aactattgta acatcaaaat
    19741 tacctggttt atttgcgtta attgtaacag ccattatttt gttgcggatt agcagaaatt
    19801 ggctatacag gggttatctg ctttatttat tcccttttgg tctgatgctt ttctggaaca
    19861 gggctgaacc ctttttaatc atgattgtgt ctatatctct tctttttggt cagaattatg
    19921 ttaataaaag atacttaccg ataataatgg gtgttcttgg cgggattgct tctgcattaa
    19981 aaatccatgg agcagcttat atctttacag cctatcttgc tgttgctgca tcaagttgtt
    20041 caatttcatc tatattgtta tttttagttt catcggtatt atctttctct gcattttttc
    20101 tacctgataa tgtattattt tccggatttt ggggttattt gagtttggtt ggaatcgacc
    20161 atggtttgtc tttaaaactt tggattgaaa acttgatata tcttgctttt ttgtcttttc
    20221 cctttgttgt attttggcgc ggcataaagc tgaaacacac tatgagacta aggataacat
    20281 taattttggt ggtagaattt ttaatcacga tagttgcttc caaatacggg aatggatttt
    20341 accatttaat gccattgata ccaatgaatg cgtttataat tcagaaacta tctgaagccg
    20401 ttgttgtgga aaaaggcttg ttcaaaatat tatatgtgtc attgattgtt gtatcattta
    20461 tagttgtacc attagatttt gttcttccta tggctaagag ctggcatcaa ttcaattatg
    20521 cacaaaaaga gatagcatct ctcgagaaaa attttccagg tttaatgatg ggggttactg
    20581 atgaacaagg ttacccatat gctttcatga gggttatgct taggaaaaga cagattgatt
    20641 atgcagcttt tatggatttg caggtggcag ggtttaaaga cgacgagttt gcagaaagat
    20701 tagaaagatg taaaatccct tcgatattga ttccaaatta tggagaacca ttttctttaa
    20761 aaaactatta taccagtgaa cctttatttt cggacaagct tcgtgaggtc tttgcctcaa
    20821 aatatatcct tattcagaga ggaaaatact actcagtata tacatgtgct ttttcatcaa
    20881 gggacatgtt gccttaaggc aatgatagaa aagttgtttc aa
//
LOCUS       DAOSHP010000026        20204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2154_length_20204_cov_9.942479, whole genome shotgun sequence.
ACCESSION   DAOSHP010000026 DAOSHP010000000
VERSION     DAOSHP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 20204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20204
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2154_length_20204_cov_9.942479"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..801)
                     /locus_tag="PLB33_04085"
     CDS             complement(82..801)
                     /locus_tag="PLB33_04085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG62735.1"
                     /translation="MKNNRFARAKEKMKDFFIRLRHKDTSFLIIALCIMLLATAVVWR
                     YTSNPENGQSDLAENNEPNGDIQVHEDPYEDYVQDIIDEFQNAEDDNDEDDGVKPEGT
                     DLKSMKSPLAGEIIREFTLDELVYYEAIGEWRVHKGIDIKPKDTLLIESALAGKVEAV
                     NNSEITATEIIIDHGNNVKTLYSNLVSANVKAGDTVEKGQVIGNVGKTVSIEASDGAH
                     LHFEVIVDGQHINPLDYITIE"
     gene            938..1972
                     /gene="spoIID"
                     /locus_tag="PLB33_04090"
     CDS             938..1972
                     /gene="spoIID"
                     /locus_tag="PLB33_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOG62736.1"
                     /translation="MLNRIIYAFVVFTMLLTIPNLSLNFFEKKMNLTSAAVQQKDSSK
                     YANEKHEEIYTADVYEPELIKVYNNKTNEVMIIDFEEYLKGVVASEMPAEFNIEALKA
                     QAVSARTYLLYRLKKYPDGQPEHSEAPVCTGVHCQVWNSKDELIRSHEEGWYDESWGK
                     IEEAVNSTRGQILVYDNKIIEPLYHSTSGGRTENSEDVFSTAVPYLRSVESPYEGDSP
                     RLNGSIKITTSEFIDKIESAYGDMDITESNLADKIVLGEVSEGGKIKTLIIDNTEISG
                     RDMRALFNLNSTNFSFIQSGNEIEILTTGYGHGVGMSQWGAEGMANRGYNYKEILKHY
                     YTGVEIVSMK"
     gene            2127..2450
                     /locus_tag="PLB33_04095"
     CDS             2127..2450
                     /locus_tag="PLB33_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB-like protein"
                     /protein_id="HOG62737.1"
                     /translation="MIFIDDKAYKEFKELLDNANVDSYNVRIAIDRVGCSGPIFGVFI
                     DEPNENDDVEVVNEITFISEKSIVEKYGGFIIVSSEENNGRGVGMKPVVSPYSGCDGC
                     GGGCH"
     assembly_gap    2451..2550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2590..2862)
                     /gene="rpsR"
                     /locus_tag="PLB33_04100"
     CDS             complement(2590..2862)
                     /gene="rpsR"
                     /locus_tag="PLB33_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOG62738.1"
                     /translation="MSSRQSGKDINDKNEKYSQRRKPRKKVCQFCQEKATHIDYKDLG
                     KLKKYTTERGKILPRRITGACAKHQRMVTRAIKRARSIALLPYVAE"
     gene            complement(2887..3345)
                     /locus_tag="PLB33_04105"
     CDS             complement(2887..3345)
                     /locus_tag="PLB33_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG62739.1"
                     /translation="MNNVVLIGRLARDPDLRFIPSTGMAVAKFPLAVDRGLYGEKKQE
                     AINQGKQTADFINITVFGKTAELCANYLTKGRQCAIQGRIQTGSYTTQTGEKRYTTDV
                     IADRVEFIGSKEQSSSPMGNAVQPDSSFFNDFPDEDNDIFEPVDDDDIPF"
     gene            complement(3362..3652)
                     /gene="rpsF"
                     /locus_tag="PLB33_04110"
     CDS             complement(3362..3652)
                     /gene="rpsF"
                     /locus_tag="PLB33_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOG62740.1"
                     /translation="MRKYEGAFILLSNLEEDVRNAEIEKIQSIITERQGTIEKVNEWG
                     QRRLAYEINKKREGYYIFIEFTAGADAVDEVDRICKISDNFIRHMITVVEEK"
     gene            complement(3743..3904)
                     /locus_tag="PLB33_04115"
     CDS             complement(3743..3904)
                     /locus_tag="PLB33_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxH/CxxC protein"
                     /protein_id="HOG62741.1"
                     /translation="MKEMYEEIFACMEHVEQAIDDYVNSKEAAPQLIKTDKKEKCSYC
                     KKNAEYIIK"
     gene            complement(3912..4700)
                     /locus_tag="PLB33_04120"
     CDS             complement(3912..4700)
                     /locus_tag="PLB33_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG62742.1"
                     /translation="MKLKFCSLYSGSSGNCQYIKTKNTTIIVDAGLSGKRIQQEIANI
                     GEDPNKIDAIFITHEHIDHIQGAGIMSRRLNIPIYANEETWEAMKSLIGDIKSCNIKI
                     LDESTQIGDLTIKSFDISHDAAHPVGFNIFYENKKISLVTDTGCFNGTITNSIMDSDL
                     LLVESNHDEDLVIIGPYPWPLKRRVLGEFGHMSNEAAGNLIKKVIKKGTEIILLGHLS
                     KENNFPELAYKTVESILLEDSIDVNPGICLDLTYRDKSSKVYEI"
     gene            4838..5881
                     /locus_tag="PLB33_04125"
     CDS             4838..5881
                     /locus_tag="PLB33_04125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG62743.1"
                     /translation="MIIKIKLKSLLIFFAIIFVVLAIYLFMNKTQSVSADEEKDFIKW
                     VDFRVSYEAMDKALRADINSLDEEVKLDWIEILAYLGTRYGGDFFSRYKAKDMDKLIN
                     RLKSGETIESMTKDLKHYNYYYEAYKAVLEGFVGPFEIQVKDEDTGEKVWVKKYGLKA
                     FSPIAGGYHYGHYDDFGNSRSYGFRRVHLGNDLVGSIGTPIIAVESGRIEALGWNQYG
                     GWRIGIRSLDNKRYYYYAHLRKDHPYVKTLKEGDIVLAGDVIGYLGMTGYSRKENVNN
                     IRTPHLHFGMQLVFDESQKEALAEIWIDVYRIVKLLNRNRSPVVYNKETKEFERIYDI
                     RFHNIERKLSSIE"
     gene            complement(6030..7448)
                     /locus_tag="PLB33_04130"
     CDS             complement(6030..7448)
                     /locus_tag="PLB33_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62744.1"
                     /translation="MKTITRKIIMIFILLLIFNTTVYAGVNPSREEIEAIIDEVATKR
                     GIPAIILKGIARVESVFKHFNSDGSPKICGTSIGLMQINNVNGGYDNYRLKYDLLYNI
                     EAGADMLLNKWSMSSYKQVSSVGNMDPNILENWYFALWAYNGWSQSNNPVSPYAKKYT
                     YQQLVYDVIEKEYGKKINNIDFSYLPRESKPSRSLVVPTPNNYSSGNIIFYEKGDYVR
                     TDGIRKAYYLRDAPAGNYIYQLNVNQLGIITEGPLLKNGYYWYKVYIDETKEGWIERN
                     WLLRTGDTQYGRYIFEDIPFHWARKIIMDMYGRGIVSKAALYNPNNPVTYEEFCIFLS
                     RTLNYLENVNKQSKIQTSNKILYYDFTNVDLWALDFVKDIYERGLLKDIQPLQRLSRK
                     TAPLLIEDILTDSDKYLDIDINMVFSDLGNLNEEEISAVKKAYVNGIISGKDIKAFCP
                     DEYLTRAEATVIMSNIIKKYIR"
     gene            complement(7650..9158)
                     /locus_tag="PLB33_04135"
     CDS             complement(7650..9158)
                     /locus_tag="PLB33_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62745.1"
                     /translation="MKILKTTARKVIIIIFVFVFTINTFVFAGVNPSREELELMIERV
                     AEKRAIPAILLKAIARVESCYEHYRADGSPKINGTSIGLMQINNKNGGYDSERLKYDI
                     MYNIEAGADVLLNKWAMSSYNEVASVGDMDPNVLENWYFALWAYNGWSQSNNPNIYQS
                     YTKKYTYQQLIYDVIEKEYGGKIHNIDFSYLPATGKPSRSLVVPTPMYTNGGNIIFYE
                     KGDYVRTDGIRTKFHLRDAPAGRYIHDLSLNQLGIIEDGPVLKNGYYWYKVYIDDNTE
                     GWIERNFLLRTGDAEYGRYVFEDISFHWARKIIMKLYGKNIVGEAEYFNPDKYVSKEE
                     FCILLNRALDYANVLNDSLGDDESKDDKDKEKSSRETETTSEEDASAILENIENINPW
                     AVEYVENVYESGLIDDEDLVNLLDNLNRKEAALIIANLFEISEEFSSLDIETVFTDIG
                     NLNEDEILAIKTVYTNGIMNGKGSGIFSPDANLTRAEAAVVMDKISERLSLQ"
     gene            complement(9189..10901)
                     /locus_tag="PLB33_04140"
     CDS             complement(9189..10901)
                     /locus_tag="PLB33_04140"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOG62746.1"
                     /translation="MYKEKFEMWLNSPHVDDVLKKELKEFEKIEIEIEDRFYRDLEFG
                     TGGMRGKIGAGTNRINLVTVSKATQGIANYLNEKYKGERISAAVAYDSRNMSKEFAEK
                     TACVFAANNIKVYIFDSLRPTPELSYAVRYFNCKAGVVITASHNPPEYNGYKVYDEFG
                     GQVVQDADKIIEKINEVKLEHIKSEEFIGNTKIQIVGNDVDTSYINDIKKLSLRNDVY
                     KNIKIIYTPLHGTGNIPVRRILDEMGYKNVFVVEEQEKPDSQFSTVSSPNPEELSSFE
                     IAIKKAKEMGGEIIIGTDPDCDRVGAIVKHKGEFIPLNGNQTGALLLNYLLASLDEQG
                     KIPDSPAVVKTVVTSEIANCIADKYNVTTFDTLTGFKYIAELMQKFEETKEYNFLFGY
                     EESFGYLAGTFVRDKDAVIASMLICEMAAYYKKQGKTLIDVLNEIYDEHGYYIEETVS
                     LNFSGVTGQSKMKAIMEHFRENKLSSLAGKKIPTIIDYKLSISLNLIKGTAERINYPK
                     SDVLKFSFEDGSWLVLRPSGTEPKLKVYFSINGHSRDESIESLEKIKSEILNIIDRIQ
                     INIG"
     gene            complement(10914..11780)
                     /gene="galU"
                     /locus_tag="PLB33_04145"
     CDS             complement(10914..11780)
                     /gene="galU"
                     /locus_tag="PLB33_04145"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOG62747.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAIPKEMLPIVDKPTIQYIVEEV
                     VASGIEDVLIITGRNKSSIEEHFDRAAELEYYLEKNNKEELLNEIKSISKMINIHTVR
                     QKDPLGLGHAIYCAKSFVGDEPFAVLLGDDVVDSQKPCLKQMLEVFEEYRSTILGVQP
                     VEWEKVHKYGIVSGSKINDRIYAVNGLVEKPGIENAPTNIAILGRYIITPKIFEILEN
                     TKAGVGGEIQLTDGLKELCNTEEIYAYIFQGRRYDVGSKIGFLEATVDYALKSDDLKE
                     GFKKYLRSINID"
     gene            complement(11798..12829)
                     /locus_tag="PLB33_04150"
     CDS             complement(11798..12829)
                     /locus_tag="PLB33_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOG62748.1"
                     /translation="MGLRMDIGIDLGTASVLVYIKGKGIVINEPSVVAIDINTNKILE
                     VGEEARKMLGRTPGNIVAIRPLRDGVISDFDITEKMLKYFIKKAVGNSFIKPRVIICV
                     PSGVTEVEKRAVLEASNNAGAKKTYLIEEPVAAAIGAGLDITKPSGHMVIDMGGGTTD
                     IAVISLGGIVVSRSIKVAGDKCDDAIVRYVRKKYNVMIGLRSAEELKIKIGTVFPVEE
                     ERFMEVRGRNLVTGLPVNLTISSSDSMEAMEETITTIADAVHSVLEKTPPELAADISE
                     KGIVMTGGGCLIHGMDKLLEKRTGLKVTIAEDAVSCVAKGTGQSLEHMDVLKDALITE
                     NIKMNYSIQ"
     gene            complement(13020..14174)
                     /locus_tag="PLB33_04155"
     CDS             complement(13020..14174)
                     /locus_tag="PLB33_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOG62749.1"
                     /translation="MNINEYTFEEPMPFGRLSIIAMKGCEEIAARIDYYLKEWRKDAN
                     GIIYSNGSGPIRTFLLDAACPRFGSGEAKGVINETVRGHDIFIISDVFNYGVKFKMYG
                     MDVPMSPDDHYQDLKRIIAAMNGKAKRITVIMPMLYEGRQHKRATRESLDCALALQEL
                     TSMGVENIISFDIHDPRVQNAIPLHGFEDFHPHYQMIKAFVRTVPDAKIDRDSMMIIS
                     PDEGGMTRCIYYSSVMELDLGMFYKRRDYSVIVNGKNPVVSHEFLGGNLEGKDVIVVD
                     DMIASGDSMIDVAGKLKERKVKNVYIFASFGLFTEGFNKFDKAYRDGIITRVFTTNMI
                     YRRPELLEKPWYTEVDMSKYIANIIDTLNYDGSLSELLDPVQKIKKLLKK"
     gene            complement(14372..15547)
                     /locus_tag="PLB33_04160"
     CDS             complement(14372..15547)
                     /locus_tag="PLB33_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62750.1"
                     /translation="MKYNDLNEDIGREELAIIAERQNEKMAALGRLSGGIAHDYNNYL
                     MSIIGNATMIQKTDELDRIYDYAERIIHISQNAAELTKKILVFSKRKSSTNKPVNLKN
                     ILDNTYEMMEFILCKEIKLIYSYDANDEFILGDESQLESMIVNLILNSKDAIINNGII
                     KIGTKDTVVYSEMALSHGEILPPGKYIQIYIEDNGLGIDDEIMFKIFEPYFTTKIKTN
                     GTGLGLSVVFGTVKSHNGFLNVTSKTNNGTRFEIFIPVRKKSHDLGDKKIPEKENNIV
                     MLVDDDINVLEIEAEMLEDLGYDVVKFNAPLEALKYYENEYKNISFSVVDIMMPGLNG
                     KELYEKMKMINEEAVVIFITGFVQQAEYEDLMMRGLTVIEKPFTYEVLSKTIAEMYL"
     gene            complement(15712..15787)
                     /locus_tag="PLB33_04165"
     tRNA            complement(15712..15787)
                     /locus_tag="PLB33_04165"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15752..15754),aa:Lys,seq:ttt)
     gene            15949..17190
                     /locus_tag="PLB33_04170"
     CDS             15949..17190
                     /locus_tag="PLB33_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG62751.1"
                     /translation="MAFNMVAEHAIWPKVNDAIFGLAAKAKEAIDKYGKENVIDSTLG
                     ALVDDNGELICLNTVYKELKSLPNSAIAAYAQVAGQPDFLEVVQKVCFGKYKPNAYIR
                     AVATPGGTGSVRHGIYNYTNPGDSILVGDWHWAPYVTISEEYGRTVTNHELFNSKNEF
                     NIESFKEKFEELLNKQKRLVAVINTPANNPTGYSLSDEEWDEVLNIAKENAKDPENRI
                     IIMVDCAYIDYAGIGDERRKFFAKFSGLPKNILIIVAFSMSKGYTMYGMRSGAAIGIS
                     SDEDIAEEFYYSCMHSNRANWSNGTRAAMEIMTRIANDPEKTKLYEAEVNETKVMLRR
                     RADAFVEGAKEVGLEILPYRDGFFVSIPCKDAKKLSDELIKENVFAIPLKKGLRFAVC
                     AVSEEKCRIAPAIIKKALDRI"
     gene            complement(17267..18517)
                     /locus_tag="PLB33_04175"
     CDS             complement(17267..18517)
                     /locus_tag="PLB33_04175"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOG62752.1"
                     /translation="MKKEKVVLAYSGGLDTSIIIPWLKENYNDLDIIACCVNVGQNDD
                     MEFVKNKALSSGASKVYIENAVNEFVEEYVYKGIKANAAYEGKYLLGTAYARPLICKK
                     LVEVAHKEGAKYIAHGCTGKGNDQVRIETGIAALDPTIEIIAPWRIWDIKSREDAIDY
                     AEAKNIDLNGITKERIYSRDQNILHVSHEGGMLENPGIEPDFEELLLMTNTVEKAPDL
                     AEYVEIEFEKGIPVKLNNKKLNGEDMLKSLNKIGGTHGIGILDLLENRLVGMKSRGVY
                     ETPGGTILLEAHKYLESFVLEKEEAHFKEIVAQKYAELVYNAMWYSGFKQALDAFIDS
                     TQEKVSGKVKLKLYKGTIKFAGMTSENALYNESISSFATGDLYDQKDATGFIHLFSLP
                     YKIQAYNKMNKNNKASDVVYSFKE"
     misc_binding    complement(18647..18856)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19043..19115)
                     /locus_tag="PLB33_04180"
     tRNA            complement(19043..19115)
                     /locus_tag="PLB33_04180"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19080..19082),aa:Val,seq:tac)
     gene            19191..19475
                     /locus_tag="PLB33_04185"
     CDS             19191..19475
                     /locus_tag="PLB33_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOG62753.1"
                     /translation="MIKVVAKGYFLEGKAEEAIKLYEELVEKTRKEEGCISYSLFRDI
                     EDDSILTMIEEWESKDALDKHFKTEHFTKLVPMIGKLRKSSEVNIYKLVI"
     gene            19657..19860
                     /locus_tag="PLB33_04190"
     CDS             19657..19860
                     /locus_tag="PLB33_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOG62754.1"
                     /translation="MKILIRRDDKNYIPMVPYMPENPKPGYGYVPYQINPRYFNNLTE
                     AFVQGTIFPDLVTPYSEFFQRGV"
     gene            19863..20138
                     /locus_tag="PLB33_04195"
     CDS             19863..20138
                     /locus_tag="PLB33_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOG62755.1"
                     /translation="MADYNRTNQRYNQQQEMLLEVQKLQFAALDTALYLDTHPNDPVA
                     LYRHKMYTDRLKEVKDAYEMQFGPMTIFNHEVGDYWRYINSPWPWEM"
BASE COUNT         6020 a         3836 c         3101 g         7147 t
ORIGIN      
        1 ttcattttta accccctttg gcattatacc gtcttattta catttttatg cgtttctttg
       61 tcctattaga acctgatttt gttattcaat ggtaatatag tccaaaggat ttatatgttg
      121 cccgtcaaca attacttcaa aatgaagatg tgcaccatct gatgcttcaa tgctgactgt
      181 ttttcccaca tttccaataa cctgaccttt ttcaacggta tcaccggctt tgacatttgc
      241 cgacactaaa ttactgtaca atgtttttac attgtttcca tgatcaataa taatttccgt
      301 tgctgtaatt tctgaattat ttacagcttc tactttccct gctaatgcgg attcaatcaa
      361 taaggtatct tttggtttga tatcaattcc cttatgtact ctccattcac caatagcctc
      421 ataatatact aattcatcaa gcgtaaattc tcttattatt tctcccgcta atggtgactt
      481 catggacttt aaatctgttc cttcaggctt aactccatca tcctcatcat tgtcatcttc
      541 tgcattttga aattcatcaa tgatatcttg tacataatct tcataaggat cttcatgaac
      601 ctgaatatct ccgtttggct cattattttc agctaaatca ctttgaccgt tttcaggatt
      661 tgaagtatat ctccatacta ctgctgttgc caacagcatg atacacaaag caattattaa
      721 gaagcttgta tccttatgtc ttagtctaat aaagaaatcc ttcatttttt cttttgctct
      781 cgcaaatcta ttgtttttca tataaacctc ccatttgata cttctttatt atctggaaat
      841 agtgtgtccc atttttttta tttaatacaa ttttgttcta aaaatttcta tttttcatag
      901 aatattaaag aaacttatga taaaggggtt ggtttttatg ttaaaccgta taatttatgc
      961 ttttgttgtg ttcacaatgc ttttaactat tcctaatcta tctttgaatt tctttgagaa
     1021 aaaaatgaat ttaaccagcg ctgccgtgca acaaaaggac agctcaaagt atgcaaatga
     1081 aaagcatgaa gaaatataca ctgcagatgt atatgaacct gaattaatca aggtgtacaa
     1141 caacaaaaca aatgaagtga tgattattga ttttgaagaa tacttaaaag gagtagttgc
     1201 ttcagaaatg ccggcagaat tcaatatcga agctcttaag gctcaggcag tttctgcaag
     1261 aacatattta ttgtatcgat taaaaaaata tccggatgga cagccggaac attcagaagc
     1321 gcctgtttgc acaggtgttc attgccaggt gtggaactcg aaggatgaat tgatcaggtc
     1381 tcatgaagaa ggatggtatg acgaaagctg gggtaaaatt gaagaagcgg taaactcaac
     1441 aaggggacaa attttagttt atgataacaa aataattgag ccgctgtatc attccactag
     1501 cggtggcaga acagaaaatt cggaagatgt attttctacg gcagttcctt atttaagaag
     1561 cgttgaaagc ccatatgaag gagattcacc cagattaaac ggctcgatta aaattacaac
     1621 cagtgaattc attgacaaaa ttgaatctgc ctacggtgat atggatataa cagaaagtaa
     1681 tttggctgat aaaattgtgc tcggtgaagt tagcgaaggt ggtaaaatca agacgttaat
     1741 aattgataac accgaaattt cgggtcgtga tatgagggca ttatttaact taaattcaac
     1801 aaatttcagc tttatacaat cgggaaatga aattgaaatt ttaactaccg ggtatggtca
     1861 cggcgtaggg atgagccagt ggggtgccga aggtatggct aatagaggct ataactacaa
     1921 ggaaatattg aaacactact atacgggtgt tgaaatagtg agtatgaaat aaaactaata
     1981 ataagattct tcactgcgtt cagaatgaca ctatgtgtta ttcatagcaa tgacaaattg
     2041 tttgacttaa aaatctgaat tatatataca attgctgctt tgtgtgatat cattgtatgg
     2101 taaaataatt ttgaaaggaa aatacaatga tatttataga cgataaagct tacaaggaat
     2161 ttaaggaatt gttggataat gcaaatgtag actcctacaa tgttagaata gcaattgacc
     2221 gtgtgggatg cagcgggcct attttcggtg tttttatcga tgaaccaaat gaaaatgatg
     2281 atgttgaagt agttaatgaa attactttca tttctgaaaa atcaatagtc gaaaagtatg
     2341 gcggattcat aattgtatcc agtgaggaaa ataacggcag gggtgtcgga atgaagcctg
     2401 ttgtaagtcc gtattccgga tgcgatggat gcggcggcgg atgccattaa nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaggattta tgtttgtgca taaatcctta
     2581 ttttttattt tattctgcta cgtacggcag taatgcaatt gatcttgctc tttttattgc
     2641 tcttgttacc attctttgat gcttagcgca agctccggta attcttctcg gtaatatttt
     2701 gcctctttca gttgtatatt tttttaactt tccaaggtct ttataatcta tgtgtgttgc
     2761 cttttcctgg cagaattggc ataccttttt acggggtttt cttctttggc tgtatttttc
     2821 atttttatcg tttatatctt tcccgctttg tctggaactc attgttctcc tccttccgca
     2881 aatatattaa aatggaatat catcgtcatc tacaggctca aatatgtcat tgtcttcatc
     2941 agggaaatca ttgaaaaaac tgctatccgg ttgtactgca tttcccatgg gtgaggaaga
     3001 ttgctccttt gagccgataa attctactct gtccgctatg acgtctgtag tgtatctttt
     3061 ttctcctgtt tgtgtagtat agctacctgt ttgaattctg ccttgtattg cgcactgtct
     3121 tcctttagta aggtaattag cgcataattc tgcagttttt ccaaatacgg ttatatttat
     3181 gaaatctgct gtctgttttc cctgatttat tgcttcttgt tttttttctc catataaacc
     3241 tctatctact gccaatggaa atttagcgac agccataccc gttgagggta taaatcttag
     3301 gtcagggtct cttgccagtc ttcctattaa tacaacatta ttcataatac taccccttta
     3361 attatttttc ttctactact gtaatcatat gtctgataaa attgtcgctt attttacaaa
     3421 ttctgtctac ttcatcaaca gcatctgctc ctgcagtaaa ttcaataaaa atataataac
     3481 cttctctttt tttgttgatt tcataagcaa gtcttctttg accccactca ttaacctttt
     3541 caattgttcc ctgtctctcg gttattatgc tttgaatttt ttcaatttct gcatttctta
     3601 catcttcctc taagttagat aataagataa aagcaccttc atattttctc atttgagtca
     3661 cctcctttgg acttttggcc cttgttcgcc aagggcagga tagcttatac attatagcac
     3721 ttgaaaaaac ctaattcaag tattatttta ttatgtattc ggcatttttt ttacaatagc
     3781 tgcatttttc ttttttatct gtcttaatca attgaggagc agcttcctta gaattcacat
     3841 aatcatctat tgcctgctcc acatgctcca tgcatgcaaa tatctcttca tacatttctt
     3901 tcatgattca cctatatttc atatactttg cttgatttat cccggtatgt taaatcaagg
     3961 cagatacccg gatttacatc gatggaatct tccaataata ttgattctac cgtcttatag
     4021 gcaagctcgg gaaagttatt ttccttgctc agatgtccaa gcaagattat ttctgttcct
     4081 ttttttataa ccttttttat taagttgcct gctgcttcat ttgacatatg tccgaattca
     4141 cccaacactc ttcttttaag cggccatgga tatggaccta tgattactaa gtcttcatca
     4201 tgatttgatt ccaccagcaa taaatctgaa tccataatgc tgtttgtaat tgtaccattg
     4261 aaacaacctg tatctgtaac taagcttatt tttttatttt cataaaatat attgaaacct
     4321 accggatgtg ctgcatcgtg ggatatgtca aatgacttga ttgttaaatc ccctatctgt
     4381 gtgctttcat ccaaaatttt aatattgcat gattttatat caccaattaa tgatttcatt
     4441 gcttcccatg tttcttcatt ggcataaatg ggaatattta atcgccttga cattattcct
     4501 gcaccttgaa tatggtcaat atgttcatgc gttatgaata tggcatctat cttattcgga
     4561 tcttccccta tatttgcaat ttcctgctga attctttttc cgcttaatcc tgcatcaaca
     4621 ataatagttg tattttttgt cttgatatat tggcagtttc cactgcttcc gctatatagc
     4681 gaacagaatt tcagtttcat tcgtcctccc ggtgtacatg aactacaatc aatcaattta
     4741 tactatcata aaagcattag ttttacaaat actttataag gttctatcca ctcattattt
     4801 gcataatatt tactatatta taatatttga ggtaattatg attattaaaa ttaaattgaa
     4861 atcattattg atattttttg ctattatatt tgttgtattg gcaatttatt tatttatgaa
     4921 taagactcaa tctgtttctg cagatgaaga aaaggatttt ataaagtggg tagatttcag
     4981 ggtttcctat gaagccatgg ataaagcttt aagggctgat ataaacagtc ttgatgaaga
     5041 agttaaattg gattggattg aaatactagc ctatcttgga acaagatatg gaggtgattt
     5101 tttttctcga tataaagcaa aagatatgga taaattaata aacagactaa aaagcggtga
     5161 aacaattgaa agtatgacca aggatctcaa acattacaat tactactatg aggcatacaa
     5221 ggctgtgctg gaaggatttg taggaccttt tgaaatacag gtaaaagatg aagacaccgg
     5281 agaaaaggtt tgggttaaaa aatacggatt aaaagcattt tcaccaatag caggaggata
     5341 tcattacgga cattatgatg atttcgggaa ctcccgcagc tacggtttcc gaagagttca
     5401 tttgggaaat gacttggttg gttcgatagg tactcccatt atcgcagttg aatcaggacg
     5461 aattgaagcc ttaggctgga atcaatacgg aggatggcga ataggaatac gcagcctgga
     5521 taacaaaagg tattactatt atgctcactt gcgtaaggat cacccttatg tcaagaccct
     5581 taaggaagga gatatcgtat tggcaggaga tgtaatcgga tatttaggga tgaccggtta
     5641 ttcgcgaaaa gaaaatgtca acaacataag gactcctcat cttcattttg gaatgcagtt
     5701 agtttttgat gagtctcaaa aggaagcatt ggcagaaata tggattgacg tatacagaat
     5761 tgtaaagctt ctgaacagaa acagatcccc tgttgtttat aataaagaga caaaggaatt
     5821 tgaaagaatt tatgatataa ggttccacaa tattgaaaga aaactatcat ccatagagta
     5881 gcgaaaatca tataatatgg ggacacacct tgagatgtac cttttgcggg tcatactttc
     5941 aaggtgtgtc cccaaatata tgagattctt cgagctaatg ctctcagaat gaaaaaaatg
     6001 ttattcatag caatggagaa gctgcagtat taacggatat atttttttat tatgttagac
     6061 attattactg tggcttctgc cctggttaaa tattcatccg ggcaaaaggc tttaatgtct
     6121 ttaccggata ttattccgtt aacgtaggct tttttaactg cagaaatctc ttcttcatta
     6181 agatttccca agtcggaaaa taccatattt atatcaatat ctaaatattt atcgctatct
     6241 gtcaatatgt cttcaattaa taaaggagct gtttttctac tgagtctttg taagggttgt
     6301 atatctttca ataaaccccg ctcgtaaatg tcttttacaa aatccaatgc ccataagtct
     6361 acattcgtaa aatcataata gagaattttg tttgatgttt gtattttgct ttgcttgttg
     6421 acattttcca aataatttaa agtcctgctc aaaaatatac aaaattcttc ataggttaca
     6481 ggattattcg ggttatatag tgccgctttg cttactattc cccttccata catatccata
     6541 ataatttttc ttgcccagtg gaatggtata tcctcaaata tatatctgcc gtattgtgta
     6601 tcccctgtac gcagaagcca atttctttca atccagcctt cctttgtttc atctatatat
     6661 actttatacc agtagtatcc gttctttagt aggggacctt cggtgattat tcccaactgg
     6721 ttaacattta gctgatatat ataatttcct gcaggagcat cccttaaata ataagccttc
     6781 ctgatgccgt ctgtccttac gtaatctcct ttttcgtaaa atataatatt accgcttgaa
     6841 tagttattag gtgtcgggac taccaatgac ctgcttggct tactttccct cggaagataa
     6901 gaaaaatcaa tattatttat ttttttaccg tattcttttt ctataacatc gtaaacaagc
     6961 tgctgatatg tatatttctt ggcgtaaggt gaaacgggat tgttgctttg tgaccaaccg
     7021 ttgtatgccc ataatgcaaa ataccagttt tccaaaatat tggggtccat atttcctaca
     7081 cttgacactt gtttgtatga actcatgctc catttgttaa gaagcatatc tgcacctgct
     7141 tctatattgt acaataaatc atacttaagc ctgtagttgt catagcctcc gtttacattg
     7201 tttatctgca tgagcccaat gcttgttccg catattttag gacttccgtc gctgttaaaa
     7261 tgcttaaaca cggactcgac tcttgctatt ccttttaata ttattgccgg tatacccctt
     7321 tttgttgcaa cctcatcaat aattgcttcg atttcttctc tagaagggtt tacacctgca
     7381 tatactgtcg tgttaaatat caataaaagt ataaaaatca ttataatctt gcgggttatg
     7441 gttttcaata tttacacctc tctgtttttg tgaaattcac aataatcttg tttataatgt
     7501 aaatattccc atattattga aaattatacc ttttaggtat gcatttattc gtaccaatat
     7561 aaaagtgaag gggatagtac ctcttttaag aagtactatc cccaatttga gattcttcac
     7621 tacgttcaga atgacaatct acggtttttt tattgcaatg acaatctttc ggatatttta
     7681 tccattacaa cggcagcctc agctctggtt aaattggcat caggactgaa tatccctgaa
     7741 cctttaccgt tcataatgcc gttggtgtag accgttttta ttgccagaat ttcatcttca
     7801 ttcaaattgc ctatatcggt aaatacagtt tctatatcta atgaagaaaa ctcttcgctg
     7861 atttcgaata aatttgcaat aattaaagct gcttcttttc tatttaaatt gtccaaaaga
     7921 ttaaccaaat cttcatcatc gattaatccc gactcataaa cattttctac atattcaaca
     7981 gcccaaggat taatattttc aatattttct aatatagcgg atgcatcttc ttcagatgtc
     8041 gtttctgttt cccgtgagct tttttccttg tctttgtcgt ctttagattc atcatctcct
     8101 aagctgtcat tcagaacatt agcatagtcc aacgctctgt ttaaaagaat gcagaattct
     8161 tcttttgaaa catatttgtc aggattaaaa tattctgctt cgccgacaat attttttccg
     8221 tatagcttca ttattatttt tcttgcccag tgaaaggata tatcttcaaa tacatatctg
     8281 ccatattcag catctccggt tcgaagcaga aagtttcttt ctatccagcc ttcagtattg
     8341 tcatctatat ataccttgta ccaataatat ccgtttttta acactggtcc gtcttctata
     8401 atacctaatt gatttaagga taagtcatgt atatacctac ctgcaggagc atccctcaga
     8461 tgaaattttg tccttatacc gtcagttcta acataatctc ccttttcata aaatattatg
     8521 ttaccgccgt ttgtatacat aggtgtggga acaaccaagg acctgctcgg tttccctgtt
     8581 gcaggaagat atgaaaaatc aatattgtgt attttcccgc catattcttt ttcaataaca
     8641 tcataaatca actgctgata agtgtatttt tttgtatatg attgatatat atttggattg
     8701 ttgctttgtg accagccatt gtaagcccat aaagcgaaat accagttttc caatacatta
     8761 gggtccatat cgcctacact tgcaacttca ttgtatgaac tcatagccca tttattcagg
     8821 agtacatcag ctccggcttc aatattatac attatgtcat atttcaacct ttcgctgtca
     8881 taacctccat ttttgttgtt aatctgcata aggcctatgc ttgtaccatt tattttcgga
     8941 ctgccgtctg ctctgtaatg ctcataacaa gattcaactc ttgctattgc ttttaaaaga
     9001 attgccggga ttgctctctt ttctgcaact ctctcaatca ttaactccag ttcttccctg
     9061 gatggattta ctcccgcaaa tacaaatgta tttatcgtaa atacgaaaac gaaaattatt
     9121 ataataactt ttcgggcagt ggttttcaat attttcatat ttacagcctc cctatttaga
     9181 ttttagtctt atccgatatt tatttgaatg cggtcaatta tatttaatat ctcgcttttt
     9241 attttttcta agctttcaat tgattcatcc cggctatgtc cgttaataga aaaatacacc
     9301 tttaatttag gctctgttcc tgagggtctc aatacaagcc aagagccgtc ttcaaaagaa
     9361 aacttcagga catccgattt cggataattt attctttcag cagtaccttt tataaggttt
     9421 aatgaaatac tcaacttata gtcaataatt gtcggaatct ttttacccgc taaggaactc
     9481 agtttgtttt ctctgaaatg ctccataatt gctttcattt tgctttgtcc ggtaacaccg
     9541 gaaaaattca gtgaaacagt ttcttctata tagtaaccgt gttcatcgta tatttcattt
     9601 aatacatcta ttaatgtctt accttgtttc ttataatatg cagccatttc acaaatgagc
     9661 atagaagcaa tcactgcatc cttatctctt acaaatgttc ctgccaaata gccgaaactt
     9721 tcttcatatc caaacaaaaa attatattct tttgtttctt caaacttctg catcaattcg
     9781 gcaatatatt taaaaccggt caatgtgtca aaagttgtta cattatattt atctgcaatg
     9841 cagtttgcaa tttcgctggt taccacagtt ttaacaactg ccggactatc aggaattttc
     9901 ccttgctcgt ccaatgaagc aagcaagtaa tttaaaagca atgctccggt ttgatttccg
     9961 tttaaaggaa taaattctcc cttatgcttt actattgctc ctaccctatc acagtcaggg
    10021 tcagtaccta taattatttc accgcccatt tctttagctt tttttatagc tatttcaaaa
    10081 gagctcagtt cttcagggtt tggtgaacta accgttgaaa attgagaatc cggtttttcc
    10141 tgctcctcaa caacaaatac atttttgtat cccatttcat ccagaattct tctcaccggt
    10201 atatttcctg taccgtgaag gggagtgtat atgattttta tatttttata aacatcattt
    10261 cttaagctta gtttctttat atcatttata taagatgtat caacatcatt tcctactatt
    10321 tgaatctttg tatttccaat aaattcttct gattttatat gctctaattt tacttcatta
    10381 attttctcaa taattttatc agcatcttga accacctgtc ctccaaattc gtcgtatact
    10441 ttgtaaccgt tatattccgg aggattatgg cttgcagtta ttactacgcc tgccttgcaa
    10501 ttaaaatacc tcacagcata agataattca ggagttggtc taaggctgtc aaaaatataa
    10561 acttttatgt tatttgcagc aaatacacat gctgttttct ctgcaaactc ctttgacata
    10621 tttcttgagt catatgcaac agctgctgaa atcctttcac ctttgtattt ctcattcaaa
    10681 taatttgcaa ttccctgagt tgctttagaa acggtaacca aatttatgcg attagttcct
    10741 gcaccaatct ttcctctcat acctccggta ccgaattcca agtccctata aaatctgtct
    10801 tctatttcta tttcaatttt ttcaaattct tttaattctt tttttagcac atcgtcaaca
    10861 tgtggactgt taagccacat ttcaaatttt tctttataca tttcccccac cccttaatct
    10921 atgtttattg accttaaata cttcttaaag ccttctttca agtcatcact tttgagagca
    10981 taatcaacag ttgcttcaag aaagccgatt ttactgccta catcatatct tctgccctgg
    11041 aatatataag catatatttc ttcggtatta cataattcct ttaacccgtc tgtaagctga
    11101 atttctcccc ccacaccggc tttcgtattt tcaagtattt caaatatttt cggagttatt
    11161 atataccttc ctaatattgc aatatttgta ggtgcatttt ctataccggg tttttcaacc
    11221 aaaccattca cggcataaat cctgtcattg attttactgc ctgacactat gccgtattta
    11281 tggacttttt cccactctac cggttgcaca ccaagtattg tagagcgata ttcctcaaaa
    11341 acttccaaca tttgctttag gcacggcttt tgtgaatcaa caacatcgtc acctaagagc
    11401 actgcaaagg gctcatcgcc aacgaaggat ttcgcacagt aaattgcatg acctaacccc
    11461 aaagggtctt tttgccttac tgtgtgaata tttatcattt tggaaatact tttaatttca
    11521 tttaaaagct cttccttatt atttttctct aagtagtatt caagctctgc tgccctgtca
    11581 aaatgctctt caattgaact cttgtttctt cctgttatta ttaatacatc ttcaattccg
    11641 gaagcaacaa cttcttctac tatgtattgt attgtcggtt tatccacaat gggcagcatt
    11701 tccttaggaa ttgctttcgt tgcaggcaaa aatctagtgc ccaaccctgc tgcaggtatt
    11761 actgcctttc taactttcat gtttacctct gcttcagcta ttgaatgctg taattcattt
    11821 ttatattttc agtaatcaat gcatctttta gaacatccat atgctccaaa gactgtcctg
    11881 ttcctttggc tacacaagat acggcatcct ctgctatggt tacttttaat ccggttctct
    11941 tttcaagcag tttatccata ccatgaatga ggcatcctcc gcctgtcatt acaataccct
    12001 tttcgctgat atcagctgca agctccggcg gagttttttc aagaactgaa tgtaccgcat
    12061 cagcaattgt tgtaattgtt tcttccatag cctccataga atctgaagat gaaatagtca
    12121 aattaaccgg aagaccggta accaaattcc tgcctctcac ttccataaat ctttcttctt
    12181 ctacaggaaa taccgttcct atttttattt taagctcttc tgcacttctt agacctatca
    12241 tgacattgta tttctttcgt acatatctta caatcgcatc gtcacactta tcaccggcaa
    12301 ccttgataga cctgcttaca actatgcctc caagagatat aacagcaata tccgttgtac
    12361 ctccgcccat gtctataacc atgtgcccgg atggctttgt tatgtctaga cctgctccta
    12421 tggcagctgc aacaggctct tcaataagat atgtcttttt tgcacctgcg ttattacttg
    12481 cttcaagaac tgctcttttt tcaacttctg taacaccgct tggaacacaa attataactc
    12541 gcggttttat aaatgaattt cctactgctt tttttataaa atatttcaac attttttccg
    12601 taatatcgaa atcagatata acaccatctc ttaagggtct aattgctaca atattgcccg
    12661 gagtacgtcc aagcattttt cttgcttctt ctcccacctc aaggattttg ttagtattaa
    12721 tatcaatagc cacaacagaa ggttcattta taactattcc cttaccctta atataaacca
    12781 atacactggc agtgcccagg tcaataccga tatccattct caggcccatc ttcatcctcc
    12841 acgtttcttc ttattatctt atctttaaaa tattaatgat aaagattata acatacaact
    12901 taatttattt caatttattt tatcccggaa atcgcacagg aatacacaaa ctaaaataaa
    12961 ttaataaata agagttatgc cctacaataa aaatgcctgc aaagcagaca ttgtatattc
    13021 tattttttaa gcaatttttt gattttttga accggatcca gtaattcgct taaggacccg
    13081 tcataattta atgtgtctat tatgtttgca atatattttg acatatccac ttcagtgtac
    13141 caaggcttct ctaacagttc cggtcttcga taaatcatgt ttgtagtaaa aactctagtt
    13201 attataccat ctcggtatgc cttgtcaaat ttattaaatc cttcggtaaa caatccaaag
    13261 gaagcaaata tatatacatt ctttaccttt ctttccttta atttgccggc aacatcaatc
    13321 attgaatcac cggaagcaat catgtcatca acaacaataa catcttttcc ttccaaatta
    13381 ccgcccaaga attcatgact gacaacagga ttcttgccat taactataac agaataatct
    13441 ctcctcttat agaacattcc taaatctaat tccataaccg atgaataata aatacatctt
    13501 gtcattccgc cttcatcggg agatattatc atcatgctgt ctctgtcaat tttagcatca
    13561 ggcactgttc ttacaaatgc ttttatcatt tgataatgcg ggtgaaaatc ttcaaatcca
    13621 tgaagaggta tagcattttg aactctcggg tcatgtatat caaaagaaat aatattttca
    13681 actcccattg atgtaagctc ttgtaatgcc aaagcacaat ccaaagattc tcttgtggcc
    13741 ctcttatgct gtctgccttc ataaagcatt ggcattataa ctgttatgcg ctttgcctta
    13801 ccattcattg cagcaattat tcttttcaaa tcctgatagt ggtcatcagg gctcatgggc
    13861 acatccatgc cgtacatttt gaatttaact ccgtaattaa atacatcaga tattataaat
    13921 atatcatgac ctcttactgt ttcgtttatt actccctttg cttcaccgga gccgaatctt
    13981 ggacaagcgg catctaaaag aaatgttcgt ataggacctg aaccatttga atatataata
    14041 ccgttcgcat ccttcctcca ttcctttaaa taatagtcta ttcttgcagc tatttcttcg
    14101 catcctttca ttgcaataat agataatctt ccaaatggca taggttcttc aaacgtgtac
    14161 tcatttatgt tcatttcttc ctcctgttta ccttatcatg ttacatttta tcaaaaaaat
    14221 atcaattgtc aaaagaatat gattaccata ttattaaatt atcttaacaa tgtctttgta
    14281 aacattgaaa ttgaagaaaa gctcaatttt cataataaaa taaccgcctt tgcttcgcgg
    14341 ttatttaaag gtggaatatt cttggatata gttataaata catttctgca attgtctttg
    14401 acagtacctc atatgtaaac ggcttttcaa ttacggtaag tcctctcatc attaaatctt
    14461 catattctgc ctgctgtaca aaaccggtta taaaaatcac cactgcttct tcatttatca
    14521 ttttcatttt ttcataaagt tctttgccgt ttaatccagg catcattata tccactacag
    14581 aaaaagatat atttttatat tcattttcat aatattttaa tgcttcaaga ggagcattga
    14641 attttactac atcatagcct aaatcttcca acatttctgc ttcaatttcc aaaacattta
    14701 tgtcatcatc aacaagcatt acaatattgt tttccttttc cggtattttc ttatcgccca
    14761 aatcatgact ctttttgcgt acaggtataa atatttcaaa ccttgtcccg ttattagttt
    14821 tgcttgtaac atttaggaaa ccgttgtggg atttcaccgt accgaacact acagacaagc
    14881 ctagacctgt cccgtttgtt ttaatttttg ttgtaaagta tggctcgaat attttaaaca
    14941 ttatttcatc atctataccc aagccgttat cctctatata aatttgaata tattttccgg
    15001 gtggcagtat ttcgccatga cttaatgcca tttccgaata aactacggta tccttggttc
    15061 ctatttttat tattccgtta ttgataattg catcctttga atttaatatc aggtttacta
    15121 tcatactttc aagctgggat tcatccccca aaataaattc atcattagca tcgtaactat
    15181 atatcaattt gatttcttta cagagaataa attccatcat ttcataagta ttatccaata
    15241 tattttttaa atttacaggt ttatttgtac tgctttttct ctttgaaaat acaagaattt
    15301 tttttgtaag ctccgctgcg ttttgtgaga tatgaattat tctttcagca taatcatata
    15361 ttctgtcaag ctcatcggtt ttttgaatca tggtggcatt acctattata ctcataaggt
    15421 aattattgta gtcatgagct attcctcccg aaagccgacc caaggcggcc atcttttcat
    15481 tttgtctttc agctataatt gccaattctt ctctgccgat atcttcgttc aaatcattat
    15541 atttcatcgc aacctcttta caaatttatt tgtatttaat atacactttt tggattaaat
    15601 attttgtaga aaattgttga aaaggtaaaa atttgcagat ataagtagtc ctctttcctt
    15661 aaataaaaag caacattcaa cgtcgcgaag atcgtcaaat gttgtttata atggtgatcc
    15721 atcggcgatt cgaacgccgg acaccttgat taaaagtcaa gtgctctgcc tgctgagcta
    15781 atggatcaat acatattaac ttttatataa tacattcctt gtcgtatctt gtcaacaact
    15841 tatttaaata catcttttag caattgccgc tgaatttttc agaatttgta tcgtattgac
    15901 ttatattttt ttaaatgtta gaattgaaga aaaaaatgag gtgatgtaat ggcatttaat
    15961 atggttgcag aacacgcaat ttggccaaaa gtcaatgatg caatctttgg gcttgcagct
    16021 aaagcaaaag aagctataga caaatatgga aaagaaaacg tgattgattc aacccttgga
    16081 gcattagttg acgataatgg agagcttatt tgcttaaata cagtatataa agaattaaaa
    16141 tctttaccga attcagcaat tgcagcatat gctcaggtgg caggtcagcc tgattttttg
    16201 gaagtagttc aaaaagtttg cttcggcaaa tacaaaccta atgcatatat cagggctgta
    16261 gcaactcccg ggggaacagg ctccgtaaga catggaatat ataattatac aaatcccgga
    16321 gattcaattt tggtaggaga ctggcattgg gctccatatg taactatttc cgaagaatac
    16381 ggcaggactg ttaccaatca tgaattattc aacagcaaga atgaatttaa tattgaatca
    16441 ttcaaggaaa aatttgaaga attgctgaat aaacaaaaaa ggctcgttgc agtgataaac
    16501 acacctgcca acaatcctac gggatacagt ctttccgatg aagaatggga tgaagtttta
    16561 aatattgcta aagaaaatgc aaaagaccct gaaaatagaa ttataataat ggttgactgc
    16621 gcatacatag attatgcagg aatcggagat gagagaagaa aattcttcgc aaaattttcc
    16681 gggcttccta aaaatatttt aattattgtt gcattcagta tgtcaaaggg atatacaatg
    16741 tatggcatgc gctcaggtgc agcaatcgga atatcttctg atgaagatat agctgaagaa
    16801 ttctattatt catgcatgca ttcaaacagg gcaaactggt ctaacggtac aagagctgca
    16861 atggaaataa tgactcgtat agccaatgac cctgaaaaaa caaagttata tgaagcggaa
    16921 gtcaatgaaa caaaggtaat gctgagaagg cgagccgatg cttttgttga aggagcaaag
    16981 gaagtgggat tggaaatttt gccttacaga gacggattct ttgtaagcat accttgcaag
    17041 gatgcaaaaa aattgtccga tgagttaatt aaagaaaatg tatttgctat accgttaaaa
    17101 aagggacttc gttttgcagt atgtgccgtt tccgaagaaa aatgcaggat agcacctgca
    17161 ataatcaaaa aagctttgga tagaatttaa tgttattaaa atattaggga cacaatatac
    17221 aatattgcgt cccttttatt tgtagatgct ctttattaat cttacattat tctttaaaac
    17281 tgtaaacaac atctgatgcc ttgttgtttt tgttcatttt gttataagcc tgaattttgt
    17341 atggcagact gaataaatgt ataaatcccg ttgcatcctt ttgatcatac aaatcaccgg
    17401 tagcaaatga tgatattgat tcattataca atgcattttc agaagtcatt cctgcaaact
    17461 taattgttcc tttatacaat ttaagtttta cttttcccga taccttttct tgggttgaat
    17521 ctataaatgc atcaagtgct tgcttaaaac cgctgtacca cattgcattg tacaccaatt
    17581 ccgcatactt ttgagcaaca atttccttaa aatgtgcttc ttctttttcc aatacaaaac
    17641 tttcaaggta tttatgggct tccaatagta tggttccgcc gggggtttcg tatactcctc
    17701 ttgatttcat gccaaccagc ctgttttcca ataaatccaa tatgccgatt ccatgggtgc
    17761 cgcctatttt atttaatgat ttaagcatat cttccccatt taatttcttg ttgtttaatt
    17821 ttacaggtat gcctttttca aattcaattt ccacatattc agccaaatca ggggcctttt
    17881 caacagtgtt tgtcattaac agcaattctt caaaatcagg ctcaataccc ggattttcaa
    17941 gcatccctcc ttcatgactt acatgaagaa tattttggtc tcgtgagtat attctttctt
    18001 ttgttattcc gtttaaatct atattttttg cttcggcata atcaattgca tcttcccttg
    18061 attttatatc ccatatacgc catggtgcaa taatttcaat tgtagggtcc aatgctgcta
    18121 ttccggtttc aatacgtacc tggtcatttc cttttcctgt acacccgtgg gcaatatact
    18181 ttgctccttc tttatgagca acttcaacaa gtttcttgca aatcaacggt cttgcataag
    18241 ctgtccctaa aagatatttc ccttcatatg ccgcatttgc cttaattcct ttataaacat
    18301 attcttcaac aaactcattt actgcatttt cgatatatac cttgcttgca ccggaagata
    18361 atgctttatt cttaacaaat tccatatcat cgttctgacc tacatttacg cagcaagcaa
    18421 taatatctaa atcattgtaa ttctctttca gccaagggat tattattgat gtgtccaatc
    18481 ctcctgaata tgctaatacc actttttctt ttttcatttt ctgctcctcc aaatttaaat
    18541 aaaactaatt ataattgcaa tgtatgcttt cttcaaagac tatcctcact tcagagctgt
    18601 gtcccaatac cttcatcaac gtgtcagggc aaaaaaaaac tgcctataaa gaggcagtgt
    18661 ttacaccgcg gtaccactct tttataaagc agctgcttcg ctctttcatg ataacggtct
    18721 ttaaccgtat tctcatactg ctacttctga gaatcttttc atggactctt ttcaattaaa
    18781 ctacactacc gaactcacac tctgctccgg ctcgctgaag ctttatttaa tctactcttc
    18841 caatcataaa attatacaat taataatttt tctaaaatta tatgttgctt ttcttatttt
    18901 gtcaacatat ttttttattt tttttctaaa attattatat ttattcttta accggaatat
    18961 tcgttaattt tagatttact tagatccgat ttttatgata atatatagta taattaaaaa
    19021 tacggcacat gccgtatatt aatgggagct atagggctcg aacctatgac cccctgcttg
    19081 taaggcagat gctctcccag ctgagctaag ctcccgtata ataaaaatac cacaacttca
    19141 taaggttgtc aaaacatttt taatatttta taaaaaaata aggaggacgt atgattaaag
    19201 tagtagctaa aggttacttt ttagaaggca aagcagaaga agcaataaaa ctatacgaag
    19261 agttagttga aaaaaccaga aaagaagaag gatgcatttc ctacagttta ttcagggata
    19321 ttgaagatga ttcaatacta acaatgatag aggaatggga gagcaaagat gccttggata
    19381 aacattttaa aacagaacat ttcacgaaac tggttccaat gataggaaaa ctaagaaaaa
    19441 gcagtgaagt aaatatttat aaattagtaa tttaagaata ccttcttata caagatgatt
    19501 caaccgaatc atcttgtatt tttttataac aaatagggac taaaaatgag aatagttcat
    19561 taacaacatt tgaaaattgc gtcacttttt gtctatttat gaataatatg aaataactat
    19621 ttttttaacg aaatagcaaa tattcatagg aaaggaatga aaattttgat tagaagagat
    19681 gataaaaatt atataccgat ggtgccatat atgcctgaaa acccaaaacc gggatacgga
    19741 tatgttcctt atcaaataaa tcctcgttat tttaacaatt taactgaggc ttttgttcag
    19801 ggtacgatat ttccggattt ggttactcca tattcagaat tttttcaaag aggggtgtaa
    19861 ttatggcaga ttataacaga acaaatcaaa gatataatca acagcaggaa atgttgttgg
    19921 aagtacaaaa acttcaattt gcagcattag atactgcact ttatttagat actcacccga
    19981 atgaccccgt tgccctgtat agacataaaa tgtacaccga caggttaaaa gaagttaagg
    20041 atgcatatga aatgcaattt ggaccaatga ctatatttaa ccatgaagtt ggtgactatt
    20101 ggcgatatat aaacagcccg tggccttggg aaatgtaagt agattatata gaaaggaatg
    20161 ataactctat gtttatgtat gaaaaaaaat tacagtatcc tgta
//
LOCUS       DAOSHP010000027        19921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2202_length_19921_cov_9.425803, whole genome shotgun sequence.
ACCESSION   DAOSHP010000027 DAOSHP010000000
VERSION     DAOSHP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19921
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_19921_cov_9.425803"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /gene="rpsJ"
                     /locus_tag="PLB33_04200"
     CDS             <1..281
                     /gene="rpsJ"
                     /locus_tag="PLB33_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOG62756.1"
                     /translation="KAYDHQLIDQSAQKIVETAKSTGATVAGPIPLPTEKQIITILRA
                     VHKYKDSREQFEQRTHKRLIDITNPTPKTVDSLMKLNLPAGVDIEIKL"
     gene            413..1045
                     /gene="rplC"
                     /locus_tag="PLB33_04205"
     CDS             413..1045
                     /gene="rplC"
                     /locus_tag="PLB33_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOG62757.1"
                     /translation="MKAILGKKIGMTQVFNEQGEVIPVTVLESEGMVVVQKKTEEKDG
                     YNAIQVGFGDVKIKNVIKPKKGHFDKSKVEPKRFLREFRVDNIDDYEVGQKIGVDIFQ
                     AGEKVDVTGISKGKGFQGSIKRHGHARGDMSHGSKFHRLRGSLGASAGVGKVVKGTKA
                     HGHMGNEKVTVLNLEVVKVDADKNVILLKGAVPGPKKGLVKIREAVRKSK"
     gene            1072..1695
                     /gene="rplD"
                     /locus_tag="PLB33_04210"
     CDS             1072..1695
                     /gene="rplD"
                     /locus_tag="PLB33_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOG62758.1"
                     /translation="MPKVALYDITGSQIGDVELSDDIFGVKVNTHVMYEAVKNYLANQ
                     RQGTQSAKTRGEVRGGGRKPWRQKGTGRARQGSIRAPQWIGGGVVFAPKPRDYSYKLP
                     KKIKRLALKSALSSKVRDNEIIVVDSLVLDKPKTKDMVKILSNLKAGKKTLVVIPERD
                     ENVILASRNIPGVKTAYVNTINVYDILNCDSFLITKDAVNKVEEVYA"
     gene            1695..1985
                     /gene="rplW"
                     /locus_tag="PLB33_04215"
     CDS             1695..1985
                     /gene="rplW"
                     /locus_tag="PLB33_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOG62759.1"
                     /translation="MRDPHDIIIKPIVTEASMNAMADKKYTFVVDKKSNKTEIKNAVE
                     SIFGVKVASVNTMNMLGKKKRMGVHVGKRPDWKKAIVTLTEDSKTIEFFEGI"
     gene            2015..2848
                     /gene="rplB"
                     /locus_tag="PLB33_04220"
     CDS             2015..2848
                     /gene="rplB"
                     /locus_tag="PLB33_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOG62760.1"
                     /translation="MGIRKYKPTSPALRQMTVPTFEEITTDEPEKSLLTPLKSKAGRN
                     AHGRITVRHKGGGEKRKYRIIDFKRNKDGVPGKVATIEYDPNRSSYIALINYVDGEKR
                     YIIAPNKLQVGDTIVSGEDADIKIGNALPVRNIPVGTMIHNIELKPGKGGQMVRSAGN
                     AAQLMAKEGKYAQVRLPSGEVRMISMDCRATIGQVGNIDHENITIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPVTPWGQPTIGYKTRKKNKKSDKMIVSRRNK
                     K"
     gene            2875..3156
                     /gene="rpsS"
                     /locus_tag="PLB33_04225"
     CDS             2875..3156
                     /gene="rpsS"
                     /locus_tag="PLB33_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOG62761.1"
                     /translation="MGRSLKKGPYCEPSLMKKIVEMNDANNKKVLKTWSRRSTIFPEM
                     IGHTLAIHDGRKHVPVYITEDMVGHKLGEFAPTRTYRGHDKSDKTSKVK"
     gene            3174..3509
                     /gene="rplV"
                     /locus_tag="PLB33_04230"
     CDS             3174..3509
                     /gene="rplV"
                     /locus_tag="PLB33_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOG62762.1"
                     /translation="MEAKAVAKYIRISPRKMKFVCDMVRGKQVDEALAILKFHPGKGS
                     RILEKVVKSAAANAENNFDMDRDKLYVVNAYANQGPTLKRWRPRAKGSAYKILKRSSH
                     VGAVVKERE"
     gene            3531..4268
                     /gene="rpsC"
                     /locus_tag="PLB33_04235"
     CDS             3531..4268
                     /gene="rpsC"
                     /locus_tag="PLB33_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOG62763.1"
                     /translation="MGQKVNPHGLRVGIINDWDSKWYADKKNFAENLTEDYKIREYIK
                     RENYQAGIAKIEIERFAGRVKVSVFTAKPGMIIGKGGTGVDILKNSIEKITGKTVIIN
                     VEEVKVPELNGQLVAENIASQLEKRISFRRAMKQAMQRTMKVGAKGIKTSVSGRLNGA
                     DMARTEGYSEGKIPLQTLRSDISYGFAEADTTFGKVGVKVWICRGEILRKPGSLLSDY
                     QEPTRPLNESKDNKKKRRSNYKRDKKQ"
     gene            4295..4738
                     /gene="rplP"
                     /locus_tag="PLB33_04240"
     CDS             4295..4738
                     /gene="rplP"
                     /locus_tag="PLB33_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOG62764.1"
                     /translation="MLMPKRVKRRKQQRGRMTGIATRGNTITYGEYGLQAIEPAWITS
                     NQIEAARRAMSRAVKRGGQIWIKIFPDKPVTKKPAETRMGKGKGSPEYWVAVVKPGRV
                     LFEMSGVTEDVAREAMRLAMHKLPIKCKFVAKDTAAEKDGEADES"
     gene            4728..4931
                     /gene="rpmC"
                     /locus_tag="PLB33_04245"
     CDS             4728..4931
                     /gene="rpmC"
                     /locus_tag="PLB33_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOG62765.1"
                     /translation="MKAKDLRKKSVEELNKSVVDLKTELFNLRFQLATGELDNPMRIN
                     KVKKDIARVKTVIRERELNINVE"
     gene            4950..5204
                     /gene="rpsQ"
                     /locus_tag="PLB33_04250"
     CDS             4950..5204
                     /gene="rpsQ"
                     /locus_tag="PLB33_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOG62766.1"
                     /translation="MERGNRKVRIGKVVSDKMDKTIVVATEKLVAHPLYKKRIKMTKK
                     YKAHDEENLCKIGDTVKIMETRPLSKEKCWRLVEILERAK"
     gene            5235..5603
                     /gene="rplN"
                     /locus_tag="PLB33_04255"
     CDS             5235..5603
                     /gene="rplN"
                     /locus_tag="PLB33_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOG62767.1"
                     /translation="MIQTESRLKVADNSGAKEVLVIRVLGGSVVKYGNIGDIVVCTVK
                     SATPGGVVKKGDVVKAVIVRTKSGVRRKDGTYIKFDENAAVIIKDDRTPVGTRIFGPI
                     TRELRDGKFMKIISLAPEVL"
     gene            5616..5921
                     /gene="rplX"
                     /locus_tag="PLB33_04260"
     CDS             5616..5921
                     /gene="rplX"
                     /locus_tag="PLB33_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOG62768.1"
                     /translation="MHVKKGDKVVVIAGSDKGKIGKILENMPKKNRVVVENVNMQTKH
                     QKQTKEMQQAGIITKEGTISSSNVMLYCDKCKSGVRTESKIENGKKVRACKKCGTVL"
     gene            5946..6485
                     /gene="rplE"
                     /locus_tag="PLB33_04265"
     CDS             5946..6485
                     /gene="rplE"
                     /locus_tag="PLB33_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOG62769.1"
                     /translation="MARLKAIYNDNVVPAMMEKFQYKSIMQVPKIEKIVLNMGVGEAK
                     ENSKFLDNAMEEMTLIAGQKPVVTKAKKSISNFKVREGMSVGCKVTLRGERMYEFLDK
                     LVNIALPRVRDFRGVSKTAFDGRGNYAMGIKEQLIFPEINYDKIDKIRGMDIIITTTA
                     NTDEEARELLSLMGMPFRK"
     gene            6498..6683
                     /locus_tag="PLB33_04270"
     CDS             6498..6683
                     /locus_tag="PLB33_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOG62770.1"
                     /translation="MAKTSLKIKQKRKQKFSTREYTRCKICGRPHAYLRKYGICRICF
                     RELAYKGQIPGVKKASW"
     gene            6711..7109
                     /gene="rpsH"
                     /locus_tag="PLB33_04275"
     CDS             6711..7109
                     /gene="rpsH"
                     /locus_tag="PLB33_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOG62771.1"
                     /translation="MVMTDPIADMLTRIRNANHAKHEFVDIPASKIKKEIANILLEEG
                     YIKGFDVIDDGKQGIIRIELKYQQNKERVITGIKRISKPGLRVYVGKEDTPKVLGGLG
                     IAIISTSKGIVTDKKARKQGVGGEVICYVW"
     gene            7134..7673
                     /gene="rplF"
                     /locus_tag="PLB33_04280"
     CDS             7134..7673
                     /gene="rplF"
                     /locus_tag="PLB33_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOG62772.1"
                     /translation="MSRIGLKPVNIPANVEVKIDENNFALVKGPKGQLEQQLPKIINI
                     EVVGNQIIVSRESDEKEHRSLHGLTRTLINNMVTGVTEGYQKTLEIVGVGYRAQKQGK
                     KLVLNLGLSHPVEMEDPKGIETEVDGTNKIIVKGIDKQQVGNYAAIIRDWRRPEPYKG
                     KGIKYIDEVVRRKAGKTGN"
     gene            7694..8062
                     /gene="rplR"
                     /locus_tag="PLB33_04285"
     CDS             7694..8062
                     /gene="rplR"
                     /locus_tag="PLB33_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOG62773.1"
                     /translation="MLKKVDKNQKRMERHNRIRNKITGTPERPRLNIFKSSKHIYAQV
                     IDDATGTTLVSASTQDKELREKVAELTKQEAAKLVGKTVGERAKEKDINAVVFDRGGY
                     LYHGRVKLLAEGARESGLEF"
     gene            8078..8587
                     /gene="rpsE"
                     /locus_tag="PLB33_04290"
     CDS             8078..8587
                     /gene="rpsE"
                     /locus_tag="PLB33_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOG62774.1"
                     /translation="MEQRGRIDASQLDVKEKVISINRVTKVVKGGRNFRFSVLVVVGD
                     ENGHVGIGMAKAVEIPDAIRKAIQDAKKNMIKVPLKETTVPHEIIGRYGAGRVLIKPA
                     KEGTGIIAGGPVRAVMELAGVRDIRTKSLGSNNPRNVVNATMEALKSLKNSEEVAKVR
                     GKTVEEILG"
     gene            8599..8775
                     /gene="rpmD"
                     /locus_tag="PLB33_04295"
     CDS             8599..8775
                     /gene="rpmD"
                     /locus_tag="PLB33_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOG62775.1"
                     /translation="MATIKIKQIKSLIGKTPNQIKNMKALGLRKIGHVVEKEDTPQIR
                     GMIKKVDFMVEVIE"
     gene            8795..9235
                     /gene="rplO"
                     /locus_tag="PLB33_04300"
     CDS             8795..9235
                     /gene="rplO"
                     /locus_tag="PLB33_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOG62776.1"
                     /translation="MKLHELKPNPGSVKKRKRLGRGTATGQGKTSGRGMNGQGSRSGG
                     GTRPGFEGGNMPLYRRLPKRGFTNIFGTQYAELNVEVLNQFEDGAEITPEILKSEGIL
                     KKQLDGVKILGNGELEKKLTVKAHKFSKSAVEKIEAAGGKAEVI"
     gene            9236..10504
                     /gene="secY"
                     /locus_tag="PLB33_04305"
     CDS             9236..10504
                     /gene="secY"
                     /locus_tag="PLB33_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOG62777.1"
                     /translation="MFETLRNAWKIPDLRKRITFTFLMLVVYRLGAVIPVPGIDRAVV
                     ENMFAGTAGGLLDFFNMMSGGAFKDFTIFALNIYPYITSSIILQLLTIAIPKLEEIFK
                     AEDGKKKMAQWTRYITVVLALIQATAYSVGFFNSAIIEKSFISIVTVIIVLTAGTAFL
                     MWLGELITEKGIGNGISLLIMIGIVSQYPRDFGTVIYQFQAGAVNILEILVFLIFAFF
                     IIVGVIAIQQGERKVPVQYAKRVVGRKMYGGQSTHIPMKVLMAGVMPVIFASTLLAIP
                     QTLAFFFPNMAGGVQKYFSVAGSPGIYIYTTLNVLLIIFFTYFYTAVQFNPFEYSNTL
                     KENGGFIPGIRPGKPTAEFLNKVLNRITIAGAIALAIIATIPTLVFSFTNLKINFGGT
                     SLLIVTGVALETMKQIEAQMIMRHYKGFLK"
     gene            10519..11163
                     /locus_tag="PLB33_04310"
     CDS             10519..11163
                     /locus_tag="PLB33_04310"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOG62778.1"
                     /translation="MRAILLGPPGAGKGTQAETIVNEFSIPHISTGDIFRKNIKEGTA
                     LGKKAMEYMDKGKLVPDELTIELVKDRLLQDDCKNGFLLDGFPRTINQADALEDALES
                     MGQKLDYVINIMVRKELLVERAVGRRICKDCGQTYHMTFNKPSKEGVCDNCGGVLLQR
                     KDDTKETVENRINVYMEQTEPLIDYYTKKGIIINIDGEKPIEQVGKDIISKLRK"
     gene            11165..11911
                     /gene="map"
                     /locus_tag="PLB33_04315"
     CDS             11165..11911
                     /gene="map"
                     /locus_tag="PLB33_04315"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOG62779.1"
                     /translation="MIILKTRREIEIMKKAGRLVAQSLELVRRHIKPGVTTKELDRLV
                     EDFLKSKNAIPTFKGYNGFPFSICASVNEEVVHGFPSERQLREGDIISVDIGATFEGY
                     VGDSAKTFLVGQVDEEKRHLVETTRQCFYEGIKYARTSYRLSDISHAIQVYAESHGLS
                     VVRDYVGHGVGKKMHESPQVPNFGKPNKGPRLQEGMVLAIEPMINAGTYNVRVLENNW
                     TVVTVDGKPSAHYEHTVAITDGEPELLTIV"
     gene            11922..12215
                     /locus_tag="PLB33_04320"
     CDS             11922..12215
                     /locus_tag="PLB33_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOG62780.1"
                     /translation="MEGTTDLKIGQVVKSKAGRDKGRVFLICRVVDRQFVLVCDGDLR
                     KLNNPKRKKVKHLMIYNTVLTEFAEKLQENKNLNDAYVRRLLEPYALKEMEVE"
     gene            12219..12437
                     /gene="infA"
                     /locus_tag="PLB33_04325"
     CDS             12219..12437
                     /gene="infA"
                     /locus_tag="PLB33_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOG62781.1"
                     /translation="MSKKDVIEVEGKVLEALPNAMFIVELQNGHQILAHISGKLRMNY
                     IKILPGDTVACELSPYDLTRGRITWRKK"
     gene            12450..12563
                     /gene="rpmJ"
                     /locus_tag="PLB33_04330"
     CDS             12450..12563
                     /gene="rpmJ"
                     /locus_tag="PLB33_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOG62782.1"
                     /translation="MKVRPSVKPLCEKCKIIRRKGKVMVICENPRHKQKQG"
     gene            12610..12978
                     /gene="rpsM"
                     /locus_tag="PLB33_04335"
     CDS             12610..12978
                     /gene="rpsM"
                     /locus_tag="PLB33_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOG62783.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIYGIGRPTSIKILKATGINPDTR
                     VKDLTEDEIQKLRTEIDKHPVEGDARREVALNIKRLKEINCYRGLRHKKGLPVRGQNT
                     KNNARTRKGPKRVSGKKPKK"
     gene            12994..13392
                     /gene="rpsK"
                     /locus_tag="PLB33_04340"
     CDS             12994..13392
                     /gene="rpsK"
                     /locus_tag="PLB33_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOG62784.1"
                     /translation="MAAKKQKTTRVRRRERKNIEKGQAHIKSTFNNTLVTLTDLQGNA
                     ISWASSGQLGFKGSRKSTPYAASMVAEEAAKKAMEHGLKTVEVYVKGPGSGREAAIRS
                     LQAAGLEVNLIKDVTPIPHNGCRPPKRRRV"
     gene            13411..14034
                     /gene="rpsD"
                     /locus_tag="PLB33_04345"
     CDS             13411..14034
                     /gene="rpsD"
                     /locus_tag="PLB33_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOG62785.1"
                     /translation="MARYTGPVCRICRREGLKLYLKGDRCYTDKCSIGRRNYAPGQHG
                     QNNKKLTNYGTQLREKQKVRRYYGVLEGQFKEYYNIAEKMSGKTGDNLLKLLELRFDN
                     VIYRLGFANSRPEARQLVVHGHFTVNGKKADIPSMILQVGDEIKVKEKSQNSDKFKIM
                     AENHKNTAPKWLQVEPENFTGRIIAEPTKEDIEIPIEEHMIVEFYSK"
     gene            14086..15033
                     /locus_tag="PLB33_04350"
     CDS             14086..15033
                     /locus_tag="PLB33_04350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOG62786.1"
                     /translation="MIEIEKPNITLNEKNENNTYGKIVVEPLERGYGTTIGNSLRRIL
                     LSSLPGAAVTSINIQGVLHEFSTIPGVREDVTEIILNIKNLAAKMNVPGPVQLLIDAK
                     GPKEVTAADIITGGDVDIINEDLHIATLEEGSELIIEMEMEQGRGYAIAERNKKDSQA
                     IGVIPIDSIYTPVKKVNFTVEDTRVGNRTDFDKLTLEVWTNGTIEPEEAVSLGAKILN
                     EHLNLFISLTEHVNEVEIMVEKEEDEKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LTYKTEEDMMKVRNLGKKSLDEVSKKLEELGLSLRKNDN"
     gene            15052..15393
                     /gene="rplQ"
                     /locus_tag="PLB33_04355"
     CDS             15052..15393
                     /gene="rplQ"
                     /locus_tag="PLB33_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOG62787.1"
                     /translation="MGSHRKLGVKSDHRMAMLRNMTADLITYGRITTTVTRAKELRRV
                     AERIITLGKRGDLHARRQALAYLYDKNAVYKLFEEVAPKYKDRNGGYTRILKLGPRRG
                     DGAEMAIIELV"
     gene            15662..16369
                     /locus_tag="PLB33_04360"
     CDS             15662..16369
                     /locus_tag="PLB33_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOG62788.1"
                     /translation="MVNIAVCDDSKEYVEIVRELINVIMVKNNTACNIYTYISGLSLV
                     QDFRVKTFDIIFLDMEMPEIDGIKTGLLIREISDKVIIFYLTSHKEYAYESYQVKAKN
                     YLLKPIQSDVLEKELMECIAELDMPLEFLDVKDTDGIFHRIPLCDITHILRKKEDRKI
                     HIYTLDKQEIIAVQTLKNIENSLSSISFIKRSCKSCLVNLNNIRAIHKNIIVFINGET
                     EEASRRCLSDLVNLFKK"
     gene            16385..17737
                     /locus_tag="PLB33_04365"
     CDS             16385..17737
                     /locus_tag="PLB33_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOG62789.1"
                     /translation="MDSWILIEAFVNFLQNYIIYKATGLFYDRRLSFKFSVEIVIVIM
                     TLILTGLNYYSQIHSNPLLYFAYCLLLLVTAAIIYKGNIISKAVIIFLLIVVTGILEL
                     LAAVLITTLGGFDINTIQEQNNVRLGVMIISQTLYVYFYMFMRKRFGKDKISFFNNKY
                     NILVGLILVLTVIIMIILAWMYGNIDDTDDSIGKTLLMLTLCISLWSIASITLINQVL
                     KDMEEKHKNEMELQQINLERVYLTDVNAALEEIRILRHDMRGELALIHGYNELNQKDK
                     IRLHIEKKLHEMDVQLIPQIDSENIITSFLNFKIKEAESKNINVELTSNINADEEIYI
                     DKGDICRIINNIMNNAIEALQDCDTKNMKLFIGTAGGYLIIKSENPYKGSLLTEGKKI
                     ITIKKDKTKHGYGLKSINSIAEKYNGFMSITYDDNIFKIDVEMLNSEMYKNASVEFTV
                     "
     gene            17832..17966
                     /locus_tag="PLB33_04370"
     CDS             17832..17966
                     /locus_tag="PLB33_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HOG62790.1"
                     /translation="MKKSLSLLSCIAMLLVTTAQTSMGVTCLFFVNQPKVPQCLLEND
                     "
     gene            17959..18519
                     /locus_tag="PLB33_04375"
     CDS             17959..18519
                     /locus_tag="PLB33_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HOG62791.1"
                     /translation="MINKLSAKLSEGIGLRLNSSDNEKEIYAYSIEILISLLINFIIL
                     SITAYILKKQVELIVFIVFFSSLRSYAGGYHAKTHIECITLSFCLFVISAISSTYFIE
                     YGKTILFLGIIFSIIMVFVFAPTESENKPLSKKKFKKFRLISRILVIALSLTAICLYL
                     TKETMGHVYITAAVAMSFESISLLKH"
     gene            18625..19746
                     /locus_tag="PLB33_04380"
     CDS             18625..19746
                     /locus_tag="PLB33_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOG62792.1"
                     /translation="MIPGILYTVKSGDSLRDIADRFSIPFRELISTNKIINPELIYPG
                     VTLIIPQVRKPPIEVNGISYVFGNTDVPVLSNSMKYLTYLTPFSYIAREDGSLFWIND
                     QQAIQLAILNNVIPIMSVVNYSYYDTGTNAAHIILNDMTVMNELIDNIISIMIKKGYR
                     GVKFDFDNILPEDRDAYNNFLHVAGSRLNTEGFYISVALMPKFISPDYLAYDYEALGR
                     IVDFVLLKTYNWGLIEGPPAPIIPINDLRNVLDFALSIIPREKIFIGLPLYAKDWIIP
                     YVSGREAEIISAQTAIIRAQDNNAQIQYDEISQSPFFYYSDEQGRMHEVWFEDPRSLK
                     AKFDLVKEYGIRGITYWNLLYEFIQNWHLLEDNFIIVRD"
BASE COUNT         7592 a         2804 c         4397 g         5128 t
ORIGIN      
        1 taaaggctta tgatcatcaa ttaattgatc aatctgcgca aaaaatcgtg gaaacagcta
       61 aaagtaccgg agcaacagtt gcaggtccta ttccgctgcc cacggaaaaa caaataataa
      121 caatacttag agcagttcac aagtataaag attctagaga gcagtttgag caaagaacac
      181 acaaaagatt gatcgacata acaaatccta ctccaaagac tgttgattcg cttatgaagt
      241 tgaatttgcc tgcaggggta gacattgaaa tcaaattata aggatataca tcgttgaaac
      301 ggtgtgatgg aattgcatgg caatttctac cgatgtttaa aaagtagaga caacacactc
      361 tgcttagaat gattgcgcaa gcaatccgct gtaagaaatt aggaggtgta aaatgaaagc
      421 aatattagga aagaaaatcg gaatgactca agtgttcaat gaacaaggag aagttattcc
      481 tgttaccgtt ttggaatctg aaggcatggt tgtcgttcaa aagaaaacgg aagaaaaaga
      541 cggatacaat gccatacagg taggattcgg agacgttaaa attaaaaatg ttattaaacc
      601 taagaaaggt cattttgata aatcaaaagt agaacctaaa agatttttaa gagaattcag
      661 agtagataac attgacgatt atgaagttgg tcaaaagatt ggcgttgata ttttccaagc
      721 aggagaaaaa gttgacgtta caggaatttc aaaaggtaag ggattccagg gatcgataaa
      781 gagacacgga catgcaaggg gcgacatgag ccacggctcc aaattccaca gattgagagg
      841 ttcattggga gcttcggcag gcgtgggaaa ggtagttaag ggaacgaaag ctcacggcca
      901 catgggaaat gaaaaagtta cagtattaaa ccttgaagtg gtaaaggtag atgctgataa
      961 aaatgtaata cttttaaaag gtgccgtacc gggacctaaa aaaggattag tgaaaatcag
     1021 agaagcagtt aggaaatcta agtaagaatt gcgtggaaag gaggattaaa gatgccaaaa
     1081 gtagctcttt atgatataac aggcagccaa ataggtgacg tagagttaag tgatgacata
     1141 tttggagtaa aagtaaacac tcacgtaatg tatgaagcag taaaaaatta tttggctaat
     1201 caaagacaag gaactcagtc tgctaagaca agaggagaag tgagaggcgg cggcagaaag
     1261 ccttggaggc aaaagggaac aggccgtgca agacaaggaa gtataagggc tccgcaatgg
     1321 ataggcggag gtgttgtatt tgctccaaaa ccgagagatt acagctataa gcttccaaaa
     1381 aagataaaaa gattagcgct taaatcagca ttaagctcaa aagtacggga taatgaaatt
     1441 attgtagtgg atagtttggt tcttgataag ccaaaaacaa aggatatggt aaaaatatta
     1501 tcaaatctta aagcaggcaa gaaaacattg gtggttattc ctgaaagaga tgaaaatgta
     1561 atattagcat caagaaatat tccgggcgta aaaacggcat atgtaaatac aattaatgtt
     1621 tacgatatat taaactgtga ttctttcctt ataactaagg atgcagttaa taaagtggag
     1681 gaggtgtacg cataatgcgc gatccacacg acattatcat aaaaccgata gtaacagaag
     1741 caagtatgaa tgctatggca gacaagaaat acacttttgt tgttgataag aagtctaaca
     1801 agactgaaat caaaaatgca gtggagtcaa ttttcggtgt taaagtagca agtgtgaaca
     1861 cgatgaacat gcttggtaaa aagaagagaa tgggagtaca tgtgggcaag agacccgact
     1921 ggaaaaaagc cattgttact ttaacagaag acagcaagac aatagaattc tttgaaggga
     1981 tataagctta aatttaaaga aggaggaaaa tctcatgggt attagaaaat ataaaccaac
     2041 ttctccggcg ttgagacaaa tgactgttcc aacatttgaa gagataacaa ctgatgaacc
     2101 tgagaaatct cttttaactc cgttgaaaag caaagcagga agaaatgcac acggaagaat
     2161 tacggtacgt cacaaaggcg gcggtgaaaa gagaaaatat agaattatag atttcaaaag
     2221 aaataaagat ggagttccgg gaaaagttgc aacaatagag tatgatccaa acagaagctc
     2281 atacattgca cttataaatt atgttgatgg agaaaaaaga tatattatag ctccaaacaa
     2341 actacaggtt ggagatacaa tcgtttcagg tgaggatgca gatattaaaa tcggcaatgc
     2401 tctccctgta agaaatattc cggttggtac aatgattcac aatatagaat taaaacccgg
     2461 aaaaggcgga caaatggttc gctcggcagg aaatgcagct cagcttatgg ctaaggaagg
     2521 aaaatatgca caggtaagac ttccaagcgg tgaagttcga atgataagca tggattgcag
     2581 agcaacaatc ggtcaggtag gaaatattga ccacgagaat ataacaatag gcaaggccgg
     2641 aagaaagaga catatgggaa tcagacctac ggttagaggc tctgtaatga atcctaacga
     2701 ccaccctcac ggcggtggtg aaggaagagc tccaatagga agaccgtcac cggttacacc
     2761 ttggggacaa ccgacaatcg gttataaaac tcgtaagaaa aataagaaat ccgataaaat
     2821 gattgtaagc agaaggaata agaaataagc aggattgaaa ggaggaaata gtgaatgggt
     2881 agatcgttaa agaaaggacc ttattgtgaa ccaagcctta tgaagaaaat tgttgaaatg
     2941 aatgatgcaa ataataagaa agtattgaag acatggtcaa gaagatcaac aattttccct
     3001 gaaatgatag gccatacatt agctatacat gatggcagaa agcacgttcc tgtatatata
     3061 actgaagata tggtcggaca caagctgggt gaatttgctc cgacaagaac atatagagga
     3121 catgataagt ccgacaaaac ttcgaaagtt aaataagaag ggaggattcg ctgatggaag
     3181 ctaaggcagt tgctaaatat atcagaatat caccaagaaa gatgaaattc gtgtgtgata
     3241 tggtaagagg aaagcaagtt gacgaagcat tggcaatttt aaaattccat cccggcaagg
     3301 gctcaagaat tttagaaaaa gtagtgaaat ctgcggcagc taatgcagag aacaactttg
     3361 atatggatag agataaatta tatgttgtaa atgcatatgc aaatcaagga ccaactctca
     3421 aaagatggag accaagagca aagggttcgg catataaaat attaaaaaga agcagtcacg
     3481 ttggtgcagt cgttaaagaa agagaataac tacagtaaag gaggtaaaag atgggtcaaa
     3541 aggtaaaccc tcatggacta agagttggta taatcaatga ttgggattca aaatggtatg
     3601 ccgacaaaaa gaatttcgca gaaaatttaa ctgaagacta taaaattcgt gaatatataa
     3661 aaagagaaaa ctatcaagca ggtatagcaa aaatagaaat tgagagattt gccggcagag
     3721 ttaaagtaag tgtatttact gctaagccgg gtatgattat cggaaaaggc ggtaccggtg
     3781 ttgatatttt aaagaacagc atagagaaaa taacaggtaa gactgtaata atcaatgtgg
     3841 aagaagttaa ggttcctgag ttaaacgggc aattagttgc agagaacatt gcaagtcagc
     3901 ttgagaagcg tatttcattc agaagggcaa tgaaacaggc aatgcaaaga actatgaaag
     3961 ttggggcaaa gggaattaaa acaagtgtat ccggaagatt gaacggggca gatatggcta
     4021 gaaccgaagg atattcagaa ggtaaaattc ctcttcaaac tttaaggtct gacataagct
     4081 acggttttgc agaagcagat acaacgtttg gtaaggttgg tgttaaggtt tggatatgca
     4141 gaggtgaaat cttaaggaaa ccgggctcat tgttatcgga ttatcaagag cctacaagac
     4201 cgctaaacga aagcaaggat aacaaaaaga aaagaagaag taattataaa agagacaaaa
     4261 agcaataaag ccctgaagga aggaggaaat aattatgtta atgcctaaaa gagtaaaacg
     4321 ccgtaaacag caaagaggca gaatgaccgg tattgcaaca agaggcaata ctatcacata
     4381 cggcgaatat ggtctgcagg caattgagcc agcttggata acatcaaacc aaatcgaagc
     4441 agccagaagg gctatgtcaa gagcagtaaa gaggggcggc cagatttgga ttaaaatatt
     4501 tccggataaa ccggtaacaa aaaaaccagc cgaaacacgt atgggtaaag gtaagggttc
     4561 accagaatac tgggtagcag tagtgaaacc gggcagagta ttatttgaaa tgtcaggagt
     4621 tacagaagat gtagccagag aagcaatgag acttgccatg cataaattac ctatcaaatg
     4681 caaatttgtt gccaaggata cggcagcaga gaaggatggt gaagcagatg aaagctaagg
     4741 atttaaggaa gaagtcagtt gaggagttga ataagtctgt tgtggattta aaaacagaac
     4801 ttttcaattt gagatttcag cttgcaacgg gggaattaga taaccctatg agaataaata
     4861 aagtcaagaa agatattgct cgagttaaga ctgtaattcg cgagcgcgaa ttaaatatta
     4921 acgtggagta aagggaagga ggatttactt tggaaagagg caacagaaaa gtaagaatcg
     4981 gtaaagtagt cagtgataaa atggataaaa caatagtagt tgctactgaa aaacttgtgg
     5041 cacatcctct ttataaaaaa agaattaaga tgaccaaaaa gtataaagca catgatgaag
     5101 agaatctgtg caaaataggt gatacagtta aaataatgga aaccagacct ttgtcaaaag
     5161 aaaaatgctg gagattggtt gaaatattag agagagctaa atagacccta agacaattga
     5221 agggaggtaa aaacatgata caaacagagt caaggttgaa agtcgcagac aattcaggtg
     5281 caaaagaggt ccttgtaatt agagtattgg gcggttcagt tgttaaatat ggaaacattg
     5341 gcgatatagt tgtatgtacg gttaaatccg caacacccgg tggagttgtt aagaaaggtg
     5401 atgtggtaaa agctgttatt gttagaacta aaagcggcgt aagaagaaaa gacggaacat
     5461 atataaaatt tgatgaaaat gctgctgtta ttataaaaga tgatagaact cccgttggga
     5521 cacgtatatt cggacccata acaagagaat taagagacgg gaagttcatg aaaataatct
     5581 cactggcacc ggaagtacta taaggaggtg taaagatgca cgtaaaaaaa ggcgataaag
     5641 tagtagttat agcaggcagc gataaaggca agattggaaa aattcttgaa aatatgccga
     5701 agaagaacag agttgtagtt gaaaatgtaa atatgcaaac taaacaccag aaacaaacaa
     5761 aagaaatgca acaggcagga attattacaa aagaaggaac aatcagctct tcaaatgtta
     5821 tgctatactg cgataaatgt aaatcaggcg tgagaactga gtcaaaaata gaaaacggta
     5881 aaaaggtaag agcatgcaaa aagtgcggta cagttttata atacgctcag gagggaggta
     5941 cgattatggc cagattaaaa gcgatatata atgataacgt agtgccggca atgatggaaa
     6001 agtttcagta taaaagcatt atgcaggtac cgaaaataga aaagatagtt ttaaatatgg
     6061 gcgttggtga agcaaaggaa aattcaaagt ttttagacaa tgctatggaa gaaatgacat
     6121 tgattgcagg tcaaaaacct gttgttacta aagccaaaaa atcaatttct aactttaaag
     6181 taagagaagg aatgtcggta ggatgcaagg ttactttaag aggagaacgc atgtacgaat
     6241 tcctcgacaa acttgtaaat attgcattac caagagttag agacttcaga ggcgtatcga
     6301 aaacagcttt tgacggcaga ggaaactacg ctatgggaat aaaagaacag ttgatttttc
     6361 ctgaaataaa ttatgataaa atcgacaaga taagaggtat ggatataatc atcacaacaa
     6421 cggctaatac tgatgaagaa gctcgcgagc tattaagctt gatgggaatg ccttttagaa
     6481 agtaagggag gattgaaatg gcaaaaacat ctcttaaaat aaaacaaaag agaaaacaaa
     6541 agttctcaac cagagaatat acaagatgca aaatttgcgg aagacctcat gcttatttaa
     6601 gaaaatatgg tatatgccgt atatgcttta gagaattagc ttacaaaggt cagatacccg
     6661 gagttaaaaa agccagttgg taaaattttt aagaaaagga ggttacttac atggtaatga
     6721 cagatcctat cgcagatatg ctaacaagaa taagaaatgc taatcatgca aaacacgaat
     6781 ttgttgatat accggcttca aagattaaga aggaaatagc taatatctta ttggaagaag
     6841 gatatattaa aggctttgat gttatagatg acggaaaaca aggtattata agaattgagc
     6901 ttaaatacca gcaaaataaa gaaagagtaa taaccggcat taagaggatt tccaagccgg
     6961 gacttagagt ttatgttgga aaagaagata caccaaaagt cttaggcgga ttgggtatag
     7021 ctataatttc tacgtcaaag ggaatcgtaa ctgataaaaa agcaagaaaa cagggagttg
     7081 gcggagaagt aatctgctac gtatggtaat tataacgaat aggaggtgcc gatatgtcaa
     7141 gaataggttt aaagcctgta aatatacctg caaatgttga agtaaaaata gatgaaaata
     7201 acttcgccct tgttaaaggt ccaaaaggtc agcttgaaca acagctgcca aagattatta
     7261 acatagaagt tgtaggaaat caaattatag tttcaagaga atctgatgaa aaagagcaca
     7321 gatcactcca cggacttaca aggacgctta tcaacaacat ggttacaggt gttacggaag
     7381 gataccaaaa aactcttgaa attgttggtg ttggatacag agcacaaaaa caaggaaaaa
     7441 aattagtttt aaatttaggg ctctcccatc ccgtagaaat ggaagacccg aaaggtattg
     7501 aaacagaagt cgatggtaca aacaaaatta ttgttaaagg tatagataag caacaagtag
     7561 gaaactatgc ggctataatc agagattgga gaagacctga accttacaag ggtaaaggaa
     7621 ttaagtacat tgacgaggtt gttagacgca aggccggaaa gaccggtaat tagtaaagaa
     7681 aggagtgagt gtattgctta aaaaagttga taaaaatcaa aagagaatgg aaagacataa
     7741 tagaataaga aataaaatca caggtactcc tgaaagaccg agattaaata tatttaaaag
     7801 ttctaagcac atatacgctc aagtaattga tgatgctacc ggtacaacat tggtcagtgc
     7861 ttcaactcag gataaagagt taagagaaaa ggtagctgag ctgacaaaac aagaagcagc
     7921 aaaattagtc ggcaaaacag taggagaaag agcgaaagaa aaagatataa atgctgtagt
     7981 ttttgacaga ggcggttatt tatatcacgg tagagttaaa cttcttgctg aaggagcgag
     8041 agaaagcgga cttgaatttt aaataaggag gttagtcatg gagcaacgtg gacgtataga
     8101 tgcaagccaa ttggatgtga aagaaaaagt tatcagcatc aaccgtgtta ctaaagttgt
     8161 aaagggtggt aggaacttcc gtttcagcgt attggttgtt gtcggtgatg aaaatggcca
     8221 tgttggaata ggaatggcaa aagctgttga aattccggat gccataagaa aagcaatcca
     8281 agatgctaaa aagaatatga ttaaagtgcc actgaaagaa actacagtgc cacacgagat
     8341 aattggcaga tatggtgcag gcagagttct tattaaaccg gcaaaagaag gtacgggaat
     8401 aatcgcggga ggaccggttc gtgcagtgat ggaacttgca ggcgttagag atatcagaac
     8461 taaatcattg ggttcaaata atccgagaaa tgtagttaat gcaacgatgg aagccttaaa
     8521 atctctaaag aactctgaag aagttgcaaa agtcagaggg aaaacagtgg aagaaatttt
     8581 aggttaagag gtgagataat ggcaacaatt aaaattaaac aaatcaaaag cttaatcgga
     8641 aaaacaccga atcaaataaa aaacatgaaa gctctaggtt tgagaaaaat aggccatgta
     8701 gttgaaaaag aagatactcc tcaaatcaga ggaatgatca aaaaggttga ctttatggta
     8761 gaggttattg aataactaca aggaggtgtt aaatatgaaa cttcatgagt taaaacctaa
     8821 tcccggcagt gttaaaaaga gaaagagatt aggaagaggt acagccacag gacaaggaaa
     8881 aacttcaggc agaggtatga atggtcaagg ctctcgttca ggcggaggta caagaccggg
     8941 atttgagggt ggaaatatgc ctctttacag gagacttcca aaaagaggat ttacaaatat
     9001 ttttggaaca cagtatgcag aattgaatgt cgaagttttg aatcaatttg aagatggtgc
     9061 agaaattact cccgaaattc ttaaatcaga agggatacta aaaaaacaac tggacggagt
     9121 taaaatactt ggaaacggtg aacttgaaaa gaagcttaca gtaaaggctc ataaattcag
     9181 caagtcagcc gtagaaaaga ttgaagctgc cggcggtaaa gctgaggtga tttagttgtt
     9241 tgagacttta agaaatgctt ggaaaatacc tgatttaaga aaaagaataa catttacttt
     9301 tttaatgctg gtagtgtata gattaggagc agtaatacct gttccgggaa ttgacagggc
     9361 agttgttgaa aacatgtttg ccggaaccgc agggggtctg ttagacttct tcaacatgat
     9421 gagcggcggt gctttcaaag attttacaat atttgcatta aatatatatc catatattac
     9481 gtcttcgatt atacttcagt tattaacaat tgctattcca aaattggaag aaatatttaa
     9541 agcagaagac ggaaagaaaa aaatggctca atggaccaga tatattaccg ttgtgttagc
     9601 attgattcaa gcgacggcat acagcgtagg gttctttaat tcagctatta ttgaaaagag
     9661 tttcatatca atagttacgg taataatagt tttaacggca ggaacagcat tccttatgtg
     9721 gttaggtgag ttgataacag aaaaaggtat cggcaacggt atatcacttc taattatgat
     9781 aggtatagta tcacagtatc cacgtgattt cggaacagta atttatcagt tccaggcagg
     9841 tgccgtaaat atattagaaa tattagtatt tttgatattt gcatttttca taatagtagg
     9901 agttatagct attcagcagg gagaaagaaa agtacctgtt cagtatgcta aaagagttgt
     9961 aggaagaaaa atgtacggcg gacaaagcac gcatattcca atgaaagtat taatggcagg
    10021 agttatgcct gtaatattcg cttcaacact tttggcaata cctcaaacat tggcattctt
    10081 cttccctaat atggcaggag gagttcaaaa atacttctcg gtagcaggaa gtcccggaat
    10141 atatatttat acaacattaa atgtattgct gattatattc tttacgtatt tctatacagc
    10201 ggtacaattc aatccgtttg aatattcaaa tacccttaag gaaaacggag gatttatacc
    10261 gggtataaga ccgggaaaac cgactgcaga atttttgaat aaagtattaa acagaataac
    10321 aatagcagga gcgattgcac ttgcaattat agcaacaata ccaactttgg tattctcatt
    10381 tacaaatttg aaaattaatt tcggaggaac atcacttttg atagtaacag gtgttgctct
    10441 tgaaactatg aagcaaatag aagctcaaat gataatgaga cattacaaag gatttttaaa
    10501 atagaagggg taaagaaaat gagagcaatt ttattgggac ctccgggagc cggtaaggga
    10561 actcaagcag aaacaattgt aaatgaattt tcaataccgc atatatccac gggagatatt
    10621 ttcagaaaga atataaaaga aggaactgct cttggaaaga aagccatgga gtacatggat
    10681 aaaggcaaat tggttcctga tgagctgaca attgaacttg ttaaggacag attgcttcag
    10741 gatgattgca agaacggatt cctattggac ggatttccaa gaactatcaa tcaggcagat
    10801 gcattagagg atgctttaga aagtatgggt caaaagctgg actatgtaat aaatataatg
    10861 gtgagaaaag aacttcttgt tgaaagagct gtaggcagaa gaatttgcaa ggattgcggt
    10921 cagacatatc atatgacatt taacaaacct tcaaaagagg gagtttgtga caactgcggc
    10981 ggagtgcttc tccaacgcaa ggatgatacc aaagaaaccg ttgaaaacag aattaatgtt
    11041 tatatggagc aaacagagcc gcttattgat tattacacca aaaagggtat aataataaat
    11101 attgacggtg agaaacccat agaacaagtt ggcaaagata ttatttctaa gttgaggaaa
    11161 tagtatgatt attctcaaaa caagaagaga aattgaaata atgaaaaagg ccggacgttt
    11221 ggtagcacag tctctcgaat tggtgagaag gcatattaaa cccggtgtta ccaccaaaga
    11281 gcttgaccgg ttggttgagg atttcttaaa atctaaaaat gccataccta catttaaagg
    11341 ttataacgga tttccctttt ccatatgcgc ttctgttaat gaagaagtag tgcatggatt
    11401 tccttcagaa aggcagttaa gggaaggcga tataataagt gtggatattg gcgcaacatt
    11461 tgaaggatat gtgggtgaca gcgctaagac tttcttagtg ggtcaagtgg atgaagaaaa
    11521 aaggcatttg gttgaaacaa ccagacaatg cttttacgaa ggcataaagt atgccaggac
    11581 ttcataccgc ctgtctgata tatcacacgc aatccaagtt tatgcagagt ctcatggttt
    11641 atcggtagta agagattatg ttgggcacgg agtagggaaa aaaatgcatg aatcaccgca
    11701 ggtacctaac ttcggaaaac caaacaaggg tccgcggctg caagaaggaa tggtccttgc
    11761 tatcgagcct atgataaatg caggaacgta taatgtaagg gttttggaaa acaactggac
    11821 tgtggtcact gttgacggca aaccatctgc tcattatgag catacagttg caataacaga
    11881 cggggaaccg gagctgttaa ctattgttta ggggtgggct gatggaaggg acaacagatt
    11941 tgaaaatcgg ccaggtagtt aagtcaaaag caggcaggga taagggaaga gtatttctta
    12001 tatgccgggt tgttgacagg caatttgtgc tggtatgtga cggggattta agaaaattaa
    12061 ataaccccaa aagaaagaaa gtgaagcatt taatgattta taatacagta ttgacagaat
    12121 ttgcagaaaa attacaagaa aataaaaatc taaatgatgc atatgttagg agattattgg
    12181 agccgtatgc attaaaagaa atggaggttg agtgatcaat gtccaagaaa gatgtaatag
    12241 aagtcgaagg caaggtcctg gaagcacttc caaatgcaat gttcatagtc gagcttcaga
    12301 acggacacca gattttagcg catatttctg gaaaactgag gatgaactac attaaaattc
    12361 ttcccggaga tacagtagct tgtgaattat caccatatga tttgactaga ggaagaataa
    12421 catggaggaa aaagtaagga ggtattacaa tgaaagtaag accatcagta aaacctttgt
    12481 gcgaaaagtg caagattatc agacgaaaag gaaaggttat ggtaatttgc gaaaatccaa
    12541 ggcacaaaca aaaacaaggt taatggataa attaaatatt aaaactacga atgtaggagg
    12601 tgtagcttaa tggccagaat cgcaggtgtt gatttgccaa gagataagag agttgaaatt
    12661 ggcttaacat atatttacgg aataggcaga ccaacttcta tcaaaatttt aaaagcaaca
    12721 ggtatcaacc ctgatacaag ggttaaggat ttgacggaag atgaaattca aaagttaaga
    12781 actgaaatag ataagcatcc cgtagaagga gatgcgagac gagaagtagc gttgaatatt
    12841 aaaagactta aagaaatcaa ctgctaccga ggattaagac acaaaaaagg attgcctgta
    12901 agaggacaaa atactaaaaa taatgcaaga acaagaaaag gtcctaagag agtttcaggt
    12961 aaaaaaccta aaaaataaaa aggagggact taagtggcag ctaaaaaaca aaaaacaacg
    13021 cgagtaagaa gaagagaacg taaaaatata gaaaaaggcc aggctcatat taagtctacc
    13081 tttaacaata cacttgttac tcttaccgac ttgcagggaa atgcaatttc atgggcaagt
    13141 tccggtcaat taggatttaa gggttcaaga aaatctacac catatgcggc aagcatggtt
    13201 gctgaagaag cagcaaaaaa agctatggag catggattga aaacagtaga ggtatatgtt
    13261 aaaggaccgg gctcaggaag agaagcagcg attcgttcac tgcaggcagc cggacttgaa
    13321 gtaaatttaa taaaagacgt tactccaata ccgcacaatg gttgcagacc gccaaagcgc
    13381 aggagagtat aatagaaacg aggtgaaaag atggcaagat ataccgggcc tgtttgcaga
    13441 atatgcagaa gagaaggttt aaagttatat ttaaaaggtg atagatgtta taccgacaaa
    13501 tgctcaatcg gaagaagaaa ttatgctccc ggtcagcatg gtcaaaataa caagaaactg
    13561 actaactatg gtactcagtt aagagaaaaa caaaaggtta gaagatatta cggagttttg
    13621 gaaggacaat ttaaagaata ctacaatatt gcagaaaaaa tgtccggaaa aacaggtgat
    13681 aatttgctga aattgttaga gcttagattt gacaatgtaa tttacagatt aggctttgca
    13741 aattcaagac ctgaagcaag acaattggta gttcacggtc attttaccgt aaacggcaaa
    13801 aaggctgata taccgtcaat gatattacaa gtcggtgacg agataaaagt aaaagaaaag
    13861 agccaaaatt cagacaaatt taaaattatg gcagaaaatc acaaaaacac tgcacctaaa
    13921 tggttgcagg ttgaacctga aaatttcaca ggaagaataa ttgctgagcc aactaaagaa
    13981 gatattgaaa taccaataga agagcatatg attgtcgagt tctactccaa gtaatagcgt
    14041 ttaagaagat taccctcgac aaacaaaaaa ttaaggaggg ttgggatgat agaaatagaa
    14101 aaaccaaata ttactttaaa tgaaaagaat gagaataaca cctacggtaa aatagtggtg
    14161 gagcccctgg aaagaggcta cgggacaaca atcggaaatt cattaaggag aatacttctt
    14221 tcatctttac cgggagcagc agttacttca ataaacattc aaggagtgtt gcatgaattt
    14281 tctacaatac ccggagtgag agaagatgtg acagaaataa ttcttaatat taagaattta
    14341 gcagctaaaa tgaatgtacc tggacctgtg cagctattga tagacgcaaa agggccgaaa
    14401 gaggttacgg cggcagacat aattacaggc ggagatgtgg acattattaa tgaagacctg
    14461 catatagcaa cacttgaaga aggctccgag ctcattattg aaatggaaat ggagcaggga
    14521 agaggatatg ctatagcaga aagaaacaag aaggatagcc aggcaatcgg agttattcct
    14581 attgattcaa tttatacgcc tgtaaaaaaa gtaaacttta cggttgaaga cacaagagtg
    14641 ggaaacagaa cggatttcga caaacttact cttgaggtct ggactaatgg tacgatagag
    14701 cctgaagaag cagtttcatt aggtgcaaaa atattgaatg agcatctcaa cctattcatt
    14761 tcattgacag aacatgttaa tgaagttgaa attatggttg aaaaagaaga agatgaaaaa
    14821 gaaaaagttt tggaaatgac aatagaggaa cttgatttat cggtaaggtc gtacaattgt
    14881 ctgaagaggg caggaataaa tactgttgag gagcttactt acaagactga agaagacatg
    14941 atgaaagtaa ggaacctggg gaaaaaatct ttggatgaag tatctaagaa acttgaagaa
    15001 ttaggtttat ctctaaggaa aaatgataat tagctttaaa aggaggacta aatgggtagt
    15061 cacagaaagc ttggggttaa atctgaccac agaatggcaa tgcttcgaaa tatgactgct
    15121 gaccttataa cttacggcag aataacaact acggttacaa gagccaaaga gttaagaaga
    15181 gtcgcagaaa gaattatcac tcttggaaaa agaggagacc tgcatgcaag acgtcaggca
    15241 ttagcttatt tatacgataa aaatgcagta tataagctat tcgaagaagt tgctcctaaa
    15301 tacaaagata gaaacggtgg atacaccaga attctaaaat tggggcccag aagaggcgat
    15361 ggagcagaaa tggctataat cgaattagta taatatagtt gtcatcctga gtaaaatcga
    15421 aggatctaaa gacaatgtca tcctgagcgt aagcgaagga tctcatgaga atctttcggg
    15481 cagacctctc agaatgacaa ttgtagagga gcggttcttt tgttcttacg ggcaaaatga
    15541 gccgttctta aattttatgg atttttgacg aatgtataaa taaacattga aataatcgta
    15601 ttattatgat attattaact agcaattaca tttcaattac acggagtaac aatggaaaaa
    15661 tatggttaat attgctgttt gtgatgattc taaagaatat gttgaaatag tcagggaatt
    15721 aataaatgta ataatggtga aaaataatac agcctgtaat atatatacat acatttcagg
    15781 cttatccctt gtacaagatt ttagagtgaa aacgtttgac ataatttttc tggatatgga
    15841 aatgcccgaa attgatggta taaaaacagg ccttttaata agggaaataa gcgacaaggt
    15901 tattattttt tacctgacat ctcataaaga atatgcatat gaatcatatc aggtaaaggc
    15961 taaaaattat ttacttaagc ctattcaaag cgatgtattg gagaaagaac tgatggagtg
    16021 cattgcagaa ttggatatgc ctcttgaatt tcttgatgta aaagacacag acggaatatt
    16081 tcaccgcatt cctttatgcg atataactca tatacttaga aaaaaagaag atagaaagat
    16141 acatatttat actttggata aacaagaaat aatagcggtt caaactctta aaaacataga
    16201 aaacagtctg tcttcaatca gttttattaa aagatcctgt aaatcctgtc ttgtaaatct
    16261 taataatata cgagcaatac ataaaaatat tattgttttt ataaacggtg aaacagaaga
    16321 agccagcaga cgatgccttt cggatttagt gaatttattt aaaaaataaa caagaggtga
    16381 tagagtggat tcatggattt taattgaggc ttttgttaat tttcttcaaa attatattat
    16441 ttataaggcc acgggactgt tttacgacag aaggttatcg tttaaattca gcgttgaaat
    16501 tgtaatagtt ataatgacct taatacttac aggattgaat tattattccc aaatacattc
    16561 aaatcctcta ttatattttg cttactgttt attattactc gtaacagcag caataatata
    16621 taaaggcaat attatttcaa aggctgtaat aatatttttg cttatagtag ttacgggaat
    16681 ccttgaattg ctggcagcag tgttgattac cacattagga ggttttgata taaacaccat
    16741 tcaagaacaa aacaatgtca gacttggagt gatgataata tcccaaactc tttatgtata
    16801 tttttatatg tttatgagga aaaggtttgg taaggataaa ataagctttt ttaacaacaa
    16861 atataatata ctagtaggct taatattagt cttgactgta ataattatga ttattcttgc
    16921 atggatgtat ggaaacattg acgataccga tgacagtata ggtaaaacct tattgatgct
    16981 gacattatgc atttcattat ggtctattgc ttctataact ttaataaacc aagttctaaa
    17041 agacatggaa gaaaaacata aaaacgaaat ggagcttcag caaatcaatt tggaaagagt
    17101 ttatttaacg gatgtaaatg ctgccttgga agaaataaga attctgagac atgatatgcg
    17161 cggagagtta gcactaatac atggttacaa tgaattaaac caaaaggata aaatcagatt
    17221 gcacatagaa aagaaacttc atgaaatgga tgtacagctt attcctcaaa tagacagcga
    17281 gaatattata acttcattct taaatttcaa aataaaggaa gcagagtcca agaatataaa
    17341 cgttgagtta acaagcaata tcaatgccga tgaagaaata tacatcgaca agggagatat
    17401 ttgcaggatt ataaacaata taatgaacaa tgcaattgaa gcattacaag attgtgatac
    17461 aaaaaatatg aagctcttta tcgggacagc gggaggctat ttaattataa aatcagaaaa
    17521 tccttataag ggcagcttat taaccgaagg caaaaaaatt attacaatta aaaaggataa
    17581 aactaaacat ggctacggct taaaaagtat taacagtata gctgaaaagt ataacggttt
    17641 tatgagtata acttatgatg ataatatttt taaaatagat gtagaaatgt taaacagtga
    17701 aatgtataaa aacgcatcag tcgaatttac cgtttgagca atagaaacaa ccgaataggc
    17761 attttcagga aaaaatgttg aattatgcca ccttttgtct tattatgttg ttaaaggaaa
    17821 gggggattat aatgaagaaa agtttatcat tattatcatg tattgcaatg cttttggtaa
    17881 caacagcaca gacatcaatg ggagtgacat gcctattctt tgttaatcag ccgaaagtac
    17941 ctcaatgcct gctggaaaat gattaataaa ttatcggcaa aattgtctga aggaattggg
    18001 cttaggctca attcttcaga caatgaaaaa gaaatatacg catatagcat tgaaatactg
    18061 atatctttat taataaattt cattatcctt tccataacgg catatatttt gaaaaagcag
    18121 gttgaactta ttgtatttat agtatttttc tcaagcctta gaagttatgc cggagggtat
    18181 catgcgaaaa ctcatatcga gtgtataacc ttatcgtttt gtttatttgt aatatcagcc
    18241 ataagcagta cttattttat tgagtacgga aaaacaatat tatttttggg aattatattt
    18301 tcaataataa tggtatttgt ttttgctccg actgaatctg aaaacaagcc tttaagcaaa
    18361 aaaaagttta agaagtttag attaatcagc aggatattag taatagcttt atctttgaca
    18421 gcaatttgtt tatatttaac gaaagaaact atggggcatg tatatataac agcagcagtg
    18481 gcgatgtctt ttgaatcaat aagtttattg aagcattaag taacataaga gatacgggga
    18541 cagttttttc ttttgagaaa aaccgtcccc aatttttttg ttaagaatat aatacagtat
    18601 attaaaatac aggagggtaa tcatttgata ccgggcattc tttacacggt caaatccggt
    18661 gactccttga gggatatagc cgatagattt tctattccgt ttagagaact tatatcgact
    18721 aacaagataa ttaatccgga attgatttat ccgggagtaa cattaataat acctcaagtg
    18781 cgcaaaccgc cgattgaagt aaacggaatc agctatgtct ttggcaatac agacgttccc
    18841 gtattgagca actcgatgaa atatttaact tatttaacac cgttctcgta tatagcaaga
    18901 gaagatggaa gtctgttttg gattaatgac caacaagcaa tccagttagc aatattaaat
    18961 aatgttatac ctattatgtc ggtagtaaat tattcatatt atgatacagg aacaaatgct
    19021 gctcatatta ttcttaacga tatgactgta atgaatgaac ttatagataa tattatcagc
    19081 attatgataa aaaagggata cagaggagta aagtttgact ttgataatat actgcctgaa
    19141 gacagagatg catacaataa ctttttacac gttgccggaa gcaggttaaa tacggaaggt
    19201 ttttatattt ctgttgcctt aatgcctaag ttcatcagtc cggattattt ggcatatgat
    19261 tatgaggcac ttggaaggat tgtcgatttt gtattattga aaacctataa ttggggattg
    19321 attgaaggtc cgcctgcacc gattatacct ataaatgatt taagaaatgt tttagatttt
    19381 gccttatcta taattccaag agaaaaaatt tttatcggac tgccacttta cgccaaggat
    19441 tggattatac cctatgtaag cggcagggaa gctgaaatta taagtgctca aacagcaata
    19501 ataagagcac aggataataa tgcacaaata cagtatgatg aaataagtca atctccgttt
    19561 ttctattatt cggatgagca gggaaggatg catgaggttt ggtttgagga cccgagaagt
    19621 cttaaggcga aattcgatct tgtcaaggaa tatggtataa ggggtataac atattggaat
    19681 ttgctgtatg aatttattca aaactggcac ttgcttgaag ataattttat aatagtaaga
    19741 gattagtatg gcatataaat gaaagagctg agtatataaa ttttaacgag gcgggtgatt
    19801 acgcctcgtt aaaacagtgt tttgtaattg ttttgcaaat tctccctttg tcgtatcaat
    19861 tccggagtta tatgaaattc atattcagga tgttgttcgg tcagcggtgt tatcatcaca
    19921 t
//
LOCUS       DAOSHP010000028        19259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2305_length_19259_cov_8.576599, whole genome shotgun sequence.
ACCESSION   DAOSHP010000028 DAOSHP010000000
VERSION     DAOSHP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19259
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2305_length_19259_cov_8.576599"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..1394
                     /locus_tag="PLB33_04385"
     CDS             129..1394
                     /locus_tag="PLB33_04385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOG62793.1"
                     /translation="MNRKVRRRKKRIKLMSWSLVLLVGVLAASFVVRGIGKDGKKDKQ
                     PLEEHPEEQIGEEASNEVKEPQPEPDVTVRIRATGDIMFHPSQLDGAYDYKTNTYNFE
                     HSFKAIKSIIREADIAIANFEGTTAGNEVYAYQGYPLFNAPDEALDAIKNAGFDILST
                     ANNHSLDTRKAGIIRTIEQIKARGMEPIGTFTSKPETRVLIKDVKGIRFAFMAYTEMV
                     NGLETVLTPEDLDVMVNIIDEEKMKEDIIYAKDNNADVIIAYLHWGDEYSRIQAQRQE
                     ILADMLFRSGVDIILGSHPHVIQPTQTLDYDGKTKFIAYSMGNFLSNQRVETLVPYGM
                     SEQVSKYTEDGIIIDIEIEKKGSTKEISIKNINYIPLWVYKGVKENGTAEHIVYPIMD
                     YIESAELDEDSKARMSRSYADTMAQMNAE"
     gene            1409..2248
                     /gene="cdaA"
                     /locus_tag="PLB33_04390"
     CDS             1409..2248
                     /gene="cdaA"
                     /locus_tag="PLB33_04390"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOG62794.1"
                     /translation="MYDIMHRMGLIMSNIGPNTIVDIGIVYYVLYRGYILIRDMRAKQ
                     LVKGIVLLVALIPISQIFELYMVEFILSNTFQVGLIALVIVFQPEIRKALEYLGRTSF
                     TLSNVEKTNETSRKIIKEIISAITSLSRQKIGALIIFEKQIGLNDIIESGTVINANIS
                     SGLLINIFIPNTPLHDGAVIIKDYTIKAAGCFLPLTENNLLSKDIGTRHRAAIGMTER
                     SDSIALIVSEETGYISYAVEGRLYRNIQNEEIESLLQSIYIENEKVNIIDKWRNRNED
                     KKR"
     gene            2229..3455
                     /locus_tag="PLB33_04395"
     CDS             2229..3455
                     /locus_tag="PLB33_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOG62795.1"
                     /translation="MKIKNDKLVIQITCLIVAFVLWMIVMLVTNQTLEQSYPNVSVTI
                     RNLSALEKSNLVIMNQDKENITVTVKATGLTEQLEKISSRDFSAYIDVLGFNEGITNA
                     KVEVVGPSGVDVVGVYPSQIACNIEGVISKVMDVTVQFEGEHAKNYYRAQTVPNPSSV
                     KITGPRSVVNSADKAVATINIEGAMDDVVKTVPVRIYDDTDNEIFLSAPIGNVKVTVP
                     IYPTKYVALEPTITGIPEEGYELVDVTVKPERVRIAAEQDVLDTFKKLTLEELDITGA
                     YNNILSTRNILNAEGLVFLDMETKPVVNAVVEKIIERDLRYSYDDIQFINKIENSDVE
                     IKETEEEITVTVRGTSTLVNSLKKEDLILTLDLSETVNGINTVNLDCISEFTFKEISL
                     SKETVTIEVSKEPLEP"
     gene            3471..4859
                     /locus_tag="PLB33_04400"
     CDS             3471..4859
                     /locus_tag="PLB33_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG62796.1"
                     /translation="MQKINFNSMDYQGMLEAVFLATQDAISVVDEKGEHILVNPAYTK
                     ITGIEHDDIIGKNALYDIEDGEESVHMKVLSTKKPVENTKLKLRPSGKTVVAQAAPIN
                     VNGKLKGSVAVLHDISTIKDLTDKLEEAEKKLRELTYKYKPEDIIGNSKAINQVRNLI
                     EKAAKVPATVLLRGESGTGKELFANSIHAQSSRSHKNFVRVNCAALTDSLLESELFGY
                     DEGSFTGALKGGKKGLFEEADKGTIFLDEISEISLNTQAKLLRVLQEKEIMRVGSNKN
                     IPVDVRVIAATNADLAKSVREGKFREDLFYRLSILPINIPPLRERIEDIPLLVKSFIA
                     KFNDEYGRNIVDISNDALDVLMSHEWPGNVRELENIIGRAIIYMNINERVIRTTHLPQ
                     LNSNKVQNTVKSTVLTDDDVSTLDRVLSKTESAYIESVYLKFDKNKTRTAKELGISLR
                     SLYYKMEKYGIF"
     gene            5017..5925
                     /locus_tag="PLB33_04405"
     CDS             5017..5925
                     /locus_tag="PLB33_04405"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HOG62797.1"
                     /translation="MNRFDELLKMAKTTVPKKIAIAAAQDEDVLMAVKSAYLEKICIP
                     ILIGNKEKIIELSQKINFNLDDIQIIESKDDNEAARTAVSMVSSGKADILMKGLVDTA
                     IILKAVLDKEIGLRTGNILSHAAVFESDRYHKLFIITDAAMNIAPSASEKKQIIENTL
                     PLCRSLNIENPKVAVICAKEKVSPKMQATVDAEILVNMNKNGEIKGCMVEGPFGLDNA
                     ISREAAALKGVKGEVAGDADILLMPNIEAGNVMYKTLTYLADSKNAGIILGAKAPIIL
                     TSRADSDEAKLYSILLAVICSKKESI"
     gene            5922..6989
                     /gene="buk"
                     /locus_tag="PLB33_04410"
     CDS             5922..6989
                     /gene="buk"
                     /locus_tag="PLB33_04410"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOG62798.1"
                     /translation="MKQLIINPGSTSTKIAVFDDVTMIFEKTIRHTSEELQPFNRVVD
                     QFQFRKEMIISSLKENNISLNELDAVVGRGGLLKPIEGGTYLVNDKLIKDLETNIKGE
                     HASNLGALLAHSIADEVNIPSFIVDPVVVDEMEDVARISGHPEFERICIWHALNQKAV
                     ARKAAIEKFGKKYDEVNFVVVHLGGGISVGAHRKGRVIDVNNALNGEGPFSPERTGSL
                     PAAQLVKMCFSGKYTEAEIYKIIAGKGGICAYLGTNSAKEVSDRANDGDDKARLIYSA
                     MAYQVAKAIGEYAVVLDGDVDAIIITGGIAYDKDFVKMIEDKVKFIAKVIVYPGEDEL
                     LALAEGGLRVLKGVEEAKTYN"
     gene            7019..7696
                     /locus_tag="PLB33_04415"
     CDS             7019..7696
                     /locus_tag="PLB33_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62799.1"
                     /translation="MVHDKRLVIVIGHYGSGKTEFAVNYAVKMKEIYENVSIADLDIV
                     NPYFRSREKREFFEEIGIKLFDSSIRNTAVDLPALPAELMGVITNPNIKSIIDVGGDP
                     VGARVLARFAEQIKNVDYDMFYVINGNRPDTSTVDGVINYLKMIEATSRLKITGLVNN
                     THMLKATTVEDVEFGHELTKKVSWETDIPIRYEAVIKETADKIKNHEIIEKLFPINLY
                     MREEWMS"
     gene            7710..7925
                     /locus_tag="PLB33_04420"
     CDS             7710..7925
                     /locus_tag="PLB33_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG62800.1"
                     /translation="MAKGKVTFNDDACKGCELCVAVCPVKIIELNKEKINNKGYSPAH
                     VTDQEKCIACGNCAIMCPDSVITVERL"
     gene            7938..8999
                     /locus_tag="PLB33_04425"
     CDS             7938..8999
                     /locus_tag="PLB33_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOG62801.1"
                     /translation="MSKVLMKGNEAVGAAAIKAGCKCFFGYPITPSSEIPEYISRKLP
                     EAGGVFLQAESEIAAINMVYGAGGAGARCMTASSSPGISLKQEGITYAAGAEIPCVIV
                     NMMRGGPGLGSIQPSQADYYQSTRGGGNGDYRTLCYAPSSIQELVDYVMLAFDKADYY
                     RNPVLIAADGMIGQIMEAVEFKEPEPIELPPKDWATTGTGGKRKPNVVNSLYLQAEEL
                     QEHNKRLEKKYKIMEENEVRYEAFGLEDAEVVCVAYGTTSRIVKSAIKQAQAKGIKVG
                     MIRPITIWPYPYDVFKEIGPNAKGILGVEMSTGQLLDDIKIGIAGRLPVYFNGTQGGM
                     VPTPGSILADIEKIAGGTK"
     gene            8999..9745
                     /locus_tag="PLB33_04430"
     CDS             8999..9745
                     /locus_tag="PLB33_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG62802.1"
                     /translation="MTIVYEKSKGLTDTPFHYCPGCTHGVIHKLVAESLVELGVLDDA
                     IGVAPVGCSVLAYNYFNCDMQEAAHGRAPAVATGIKRVHPDKVVFSYQGDGDLASIGT
                     AEIVHAAHRGEKITTIFVNNAIYGMTGGQMAPTTLVGQKTTTSPFGRDENHCGKPIRI
                     AEMLATIDGAKFVERVTVDTPANIRKAKKAIKRAFECQIKGEGFAIVEVLSTCPTNWG
                     FTPLKALEWLRDNMIPYYPIGNFRDFGEGK"
     gene            9747..10289
                     /locus_tag="PLB33_04435"
     CDS             9747..10289
                     /locus_tag="PLB33_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG62803.1"
                     /translation="MEQKVIMAGFGGQGIMAIGRLLAYAGMLEDKNVTWMPSYGPEMR
                     GGTANCAVVVSEEEIGSPLISKDGTAAIVMNLPSMIKFEKELVPGGKLIVNKSLIDVE
                     PTRNDLDVYYIPANELALELGNGKVANMVMLGAYLEITKAVEIESVLKAFVKVFGENK
                     ADLIPLNKEALLKGAQAAKK"
     gene            10496..11518
                     /locus_tag="PLB33_04440"
     CDS             10496..11518
                     /locus_tag="PLB33_04440"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOG62804.1"
                     /translation="MEKKDIKWSELGFGYIKTDKRYYSYWKDGKWDDGQLSEDNKVHI
                     SEGSTCLHYGQECFEGLKAYSTKDGGIQLFRPDENAKRMQKSCERLLMPIVPVEKFID
                     ACVQVVKANAAWVPPYGSGATLYLRPYVIGIGDNVGVKPAEEYLFGIFCTPVGPYFKG
                     GMNPVNFITTDQDRAAPNGTGKIKVGGNYAASLYSHFEAVKKGFADCIYLDPSTHTKI
                     DEVGAANFFGITKDNKFVTPRSSSILPSITRISLMQVAKDYLNMEALETDIYVDELDN
                     LKEAGACGTAAVITPIGGIMHKDKFHVFYSETEVGPVTKKLYDTLYGIQMGDIEAPEG
                     WIYKVM"
     gene            11634..12722
                     /gene="queG"
                     /locus_tag="PLB33_04445"
     CDS             11634..12722
                     /gene="queG"
                     /locus_tag="PLB33_04445"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOG62805.1"
                     /translation="MKQIIKEAAYKLGIETVGITGISDYSYLEELLTNRKINNQCCEF
                     EEQDIKKRINAKNLFPELKSIIAIGIPYGEGYKLPSCRNKGLLSVSCHGEDYHKTVNS
                     LLVKLAEEIMRHKSFKFLPCVDTSYLIDKEICRSAGIGSYGKNSLLINKNYGSFINLG
                     YLLTDIETETDIISEKDICGTCNLCVKSCPNKAIFEIGGINPKKCISYLTQTKNYIPL
                     EYRENMGRQIYGCDICQIVCPLNKEVLNKEVNTVYSDLIVDLEELLNISNKGFINKYG
                     HTAGSWRGRNIWKRNALISIGNLNIKSLYQNVKDELQNQSEIIRCYAAWSLLKLNRPL
                     AEEILYSNLKYENDYVKNEYLKLLEAKL"
     gene            12719..13000
                     /locus_tag="PLB33_04450"
     CDS             12719..13000
                     /locus_tag="PLB33_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOG62806.1"
                     /translation="MIVCSCEIEILIYESNSLKEKRQVIKSIIERIKSKFNASVAEVG
                     YNELWNRSLIGIAVVSNNKRVCDESISKIVKFIDYDERVEILSCNKEII"
     gene            12997..13635
                     /gene="nth"
                     /locus_tag="PLB33_04455"
     CDS             12997..13635
                     /gene="nth"
                     /locus_tag="PLB33_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOG62807.1"
                     /translation="MMTKTEKVYDLLKKAYPDAKCGLVFNTPLQLLISTILSAQATDK
                     SVNKVTEELYMQYPDLDSLLTLSHEEISLKIKQIGLYRTKSKNIYNMLRVIKNNYKGE
                     VPKNMDDLMSLPGVGRKTASVVLAEAFNIPAFPVDTHVFRITRRIGIAKENTADKVSD
                     EMMKKLPKYKWHLMHHLLITHGRTTCTAKSPKCTICNVNEICTYYKKVVKEK"
     gene            13717..14187
                     /gene="trmL"
                     /locus_tag="PLB33_04460"
     CDS             13717..14187
                     /gene="trmL"
                     /locus_tag="PLB33_04460"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOG62808.1"
                     /translation="MSLNIVLFEPEIPQNTGNIARTCAATGSKLHLIKPLGFSVNDKY
                     LKRAGLDYWNLVDITYYENFEEFKKTTGNNKVFISTTKESRFYTEVAYSDECYIIFGK
                     ETAGLPGYIHEEYIKNRIKIPMVQKVNARSLNLANSVNIIVYEALRQLGFPQMI"
     gene            14196..15797
                     /locus_tag="PLB33_04465"
     CDS             14196..15797
                     /locus_tag="PLB33_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOG62809.1"
                     /translation="MDIAISLVGGLGLFLFGMIYMGDGLQKAAGNKMKDILAALTKNK
                     LMGVLVGALVTGVIQSSTATTVMVIGFVNAGLMNLNQAVGIIMGANVGTTVTALLVSL
                     NITKIATFMAGVGVIVYLIAKKRKVKNLAEVIIGFGILFIGMDLMKNAMEPLQSNPIF
                     VNLITKFDNPYLGIVVGFVMTAILQSSSATTGLLIAVAATGLISFEQAYPIVFGQNIG
                     TCLTALLASIGANKTAKRAALIHLLFNVLGTILFTLFLRIPVQRLVYMAAPVYVPRQI
                     AFGHVFFNIINVIILFPFSDFIVKASEFIIRKDGRETESVTKYIDERILATHSIALIQ
                     TAKEVLHLGNLVMDQFETAKKALFENNEELAYEVFDKEKKINRLHKEILEYLVKLDKV
                     SLTSAEKDKLFVLMNATSDIERVGDHIDNIGELLLYKFENKADFSEEATNEIANMFKI
                     TMEAYKLSLDSLASAESDVCRAVVKYEDDIDKMYKTLRKNHIDRLNNNICNPNAGIVF
                     LDMISNLERIGDHSLNIAEYILEVI"
     regulatory      15931..16038
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            16166..16525
                     /locus_tag="PLB33_04470"
     CDS             16166..16525
                     /locus_tag="PLB33_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOG62810.1"
                     /translation="MIITNNDWVLNKYKDIYNVVYIDGTFKDVLIAVRDKIHIGHELL
                     THPLGSSVKPSETPYKSVIVSDNKNYTSFDSIYMIENAIITYDKFNKDKLKLISDKVK
                     EDLKLIDLTVLESALDI"
     gene            16556..17836
                     /locus_tag="PLB33_04475"
     CDS             16556..17836
                     /locus_tag="PLB33_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOG62811.1"
                     /translation="MKLELGYIFIKDIQFSDVSKVENGTLYVNKEEVKALILEDQNFK
                     TADVELAKPGESVRIMPVKDVIEPRVKVEGPGGIFPGMVSKVDTVGSGKTNVLKGAAV
                     VTTGKIVGFQEGIIDMSGTGAEYTPFSKLNNLVIICEPIDGLKQHEHEKALRFAGYKV
                     ALYLGALAKDLTPDEVEVFETPNLVEGIKMYPELPRVAYVFMLQSQGLLHDTYVYGVD
                     AKQTLTTMIYPTEVMDGAIVSGNCVSACDKNTTYHHLNNPIIYDLFKEHGKTLNFVGV
                     IITNENVYLADKERSSNWSAKLAGFLGVDGVIVSEEGFGNPDTDLIMNCKKIEAKGIK
                     TVLVTDEYAGRDGTSQSLADADVSANAVVSAGNANEVIELPPMEKIIGHVEVADVIAG
                     GFDGSLHKDGSITAELQVITGATNEMGFNKLSAR"
     gene            17853..19157
                     /gene="grdB"
                     /locus_tag="PLB33_04480"
     CDS             17853..19157
                     /gene="grdB"
                     /locus_tag="PLB33_04480"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:18894..18896,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HOG62812.1"
                     /translation="MLKVVHYINQFFANVGGEEMAHIPAEVREGVVGPGLAFQQAFKD
                     EAEIVATIICGDSYFNENMDEAKNTIIEMVKKYNPDLFIAGPAFNAGRYGVACGAISK
                     TVKDELNIPVITGMYVENPGADMFKNDIYIVSTKNSAAGMRDAVKKMAPLGLKLAKGE
                     KIGASIEEGYIPSGKRVNFFEKERGSTRAVKMLLAKLNNKEFETEFPMPDFDRVPPNP
                     AIKDITKAKIALVTSGGIVPKGNPDHIESSSASKYGKYDIEGVMDLTEETYQTAHGGY
                     DPVYANLDPDRVLPVDIMREFEKEGKIGSLHRYFYTTVGNGTAVKSAKGFAEKYVKEL
                     IADGVDAVILTSTUGTCTRCGATMVKEIERAGIPVVHMCTIVPISLTVGANRIVPTIA
                     IPHPLGNPSLSIEEEKEIRRKLLTRALKALETEVTEQTVFEK"
BASE COUNT         7041 a         2767 c         4059 g         5392 t
ORIGIN      
        1 cctggcaccg ggtaagggtg cctggcacca acatccggta tctttaattt aattttaata
       61 atttaatttc ttgcaaaatc tttcatagta ggatagaata aatacgtaat tattcataga
      121 ggtagcatat gaacagaaaa gtcagacgac gtaaaaaaag aattaaatta atgtcatggt
      181 cattagtatt attggttggt gtattagcag cttcttttgt tgtaaggggt ataggtaaag
      241 acgggaaaaa agacaagcag ccgctggagg agcatccgga agaacaaatc ggagaagaag
      301 cctctaatga agttaaggag cctcagcctg aacctgatgt gactgttaga ataagagcga
      361 caggagacat aatgtttcat ccctcacaat tggatggagc atatgattac aaaacgaata
      421 cctataattt tgaacacagt tttaaagcta taaaaagtat tatccgagaa gcagacatag
      481 caatcgctaa ttttgaagga acaacagcgg gaaatgaagt atacgcatat cagggatatc
      541 ccttgtttaa tgctcccgat gaagcattgg acgctataaa aaatgcggga tttgacatac
      601 tgtcaactgc caacaaccac agccttgata caagaaaggc aggtattata agaacaatag
      661 agcaaattaa agcaagagga atggagccaa tcggaacctt tacttcaaag cctgaaacaa
      721 gggtattaat taaggatgta aagggtataa ggtttgcatt tatggcatat acggaaatgg
      781 taaacggtct tgaaacggtt cttactcctg aagacttgga tgtaatggta aatattatag
      841 acgaagaaaa aatgaaggaa gacataatct atgccaaaga taataatgcc gatgtaataa
      901 tagcatacct tcattgggga gacgaatatt caagaattca agctcaaagg caggaaattt
      961 tggcagacat gcttttcagg tcaggagttg acataatact cggaagccat cctcatgtta
     1021 tacaacctac gcaaacattg gattatgacg gtaaaacaaa atttatagca tattccatgg
     1081 gtaatttttt atcaaatcaa agagtggaaa cattagttcc ttacggtatg tcggagcaag
     1141 tttcaaaata taccgaagat ggaattatta ttgatataga aatagaaaaa aagggcagta
     1201 caaaagaaat ttccattaaa aatattaatt atattccgtt atgggtttat aaaggtgtta
     1261 aagaaaacgg tacagccgag catattgttt atcccattat ggattatatt gaaagtgcag
     1321 aattggatga agattctaaa gcaagaatga gcaggtcata tgccgataca atggcgcaaa
     1381 tgaatgctga atagtgcggg ggattattat gtatgatatt atgcatcgaa tgggattaat
     1441 tatgtcaaat ataggtccaa ataccatagt tgatatcggt attgtttact atgttcttta
     1501 tcgaggttat attctaatca gagatatgag agcaaaacaa ttggttaagg gtattgtact
     1561 actggttgca ttaattccta tatcgcaaat atttgaattg tatatggttg aatttatatt
     1621 aagcaataca tttcaagtcg gattaatcgc gcttgttatt gtttttcaac ctgaaatcag
     1681 aaaagctctt gaatatttgg gaagaacttc ttttaccctg tcaaatgtag aaaagactaa
     1741 tgaaacttcc agaaaaatta ttaaagaaat tatatctgca ataacgtctc tttcaagaca
     1801 aaaaatcggt gcactaatta tttttgaaaa acagataggt ctcaatgata taatagaaag
     1861 cgggactgta ataaatgcca atatttcaag cggactatta ataaatattt ttattcctaa
     1921 tactcccctt cacgacggag cagttattat aaaggattat acaataaaag cagcaggatg
     1981 ttttttgcct cttaccgaaa acaaccttct cagtaaagat atagggacaa ggcatagggc
     2041 ggcaatagga atgactgaac ggtcagattc cattgctttg atagtttctg aagaaacagg
     2101 gtacatttca tatgctgtgg aaggaagact atacaggaat attcaaaatg aagaaatcga
     2161 atcattattg cagagtattt atattgaaaa tgaaaaggta aatattatag ataagtggag
     2221 aaacagaaat gaagataaaa aacgataaat tagtcattca aattacctgt cttattgttg
     2281 catttgttct gtggatgatt gtaatgcttg taactaatca aacacttgaa cagtcttatc
     2341 caaatgtgtc tgtaacaata agaaacttat ctgccctgga aaaatctaat ttagtaataa
     2401 tgaatcaaga caaggaaaat ataaccgtaa cagtaaaagc aaccggatta acagagcaat
     2461 tggaaaaaat aagcagcagg gatttttctg cctatataga tgtcttgggt tttaatgaag
     2521 gaataacaaa tgcaaaggta gaagttgtag gaccaagcgg tgttgatgtg gtaggagtat
     2581 atccttcgca aatagcatgc aacatagaag gagtaatttc taaggttatg gatgttacgg
     2641 ttcaatttga aggagaacat gctaagaact attacagagc acaaacagtt ccaaatccgt
     2701 catcagtaaa gataacagga ccaagaagtg ttgtaaattc agctgataaa gcagttgcta
     2761 caataaatat cgaaggtgcc atggatgatg tggttaagac tgttccggta aggatatatg
     2821 atgatactga caatgaaata ttcttatcgg ctccaatagg aaatgtaaag gttacagttc
     2881 cgatatatcc tactaaatat gtagctcttg aaccaacaat tacaggaata ccggaagaag
     2941 gttatgaatt ggtcgatgta accgttaaac ctgaaagagt gaggatagcc gcagagcagg
     3001 atgtattaga tacatttaag aaacttacct tagaagaact ggatattacc ggtgcataca
     3061 acaatatact ttcaaccaga aacattttga atgcagaagg tttggtattt ttggatatgg
     3121 aaactaaacc ggttgtaaat gccgtggtag aaaaaattat tgaaagagat ctaagataca
     3181 gctatgatga tattcagttt ataaataaaa ttgaaaacag tgatgtagag ataaaggaaa
     3241 cagaagaaga aataacggtt actgtgcggg gaacttcaac cttagttaat tcactgaaaa
     3301 aagaagattt aattttaacc ctggatttat cagaaacagt taatggtatt aacactgtta
     3361 atttagactg catttctgaa ttcactttca aagaaatttc attaagcaaa gaaaccgtaa
     3421 caatagaagt ttcaaaagag cctttagagc cttaggaaga ggtattaaag atgcaaaaaa
     3481 ttaattttaa cagcatggat taccaaggaa tgttagaggc ggtttttctt gcaacacagg
     3541 atgcaataag tgttgtagac gaaaaaggcg agcatatctt agttaatccg gcttatacaa
     3601 aaataaccgg aattgagcat gacgatataa taggcaagaa tgcactctat gatattgaag
     3661 acggagaaga aagcgtgcat atgaaagtgc tttcaacaaa gaaacccgta gaaaatacaa
     3721 aattaaaatt aagaccaagc ggcaagactg ttgtagctca agcagcacca attaatgtaa
     3781 atggcaaatt gaagggaagt gttgccgttc ttcatgatat ttctactata aaggacttga
     3841 ccgataaatt agaggaagct gaaaaaaagt taagagaact aacttataaa tataaacctg
     3901 aagatattat cgggaactca aaagcaataa atcaagtaag gaatctgata gaaaaggctg
     3961 ctaaggttcc ggcaactgtt ctcttaagag gcgaaagcgg tacgggcaag gaattgttcg
     4021 ctaattcaat ccatgctcaa agctcccgct cacacaaaaa ttttgtaagg gtaaattgtg
     4081 ccgcactgac tgattcgctt cttgaaagcg agcttttcgg gtatgatgaa ggctcattta
     4141 ccggagcatt aaagggaggc aaaaagggtc ttttcgaaga ggcagacaaa ggtaccatat
     4201 tccttgatga gatttctgaa atcagtttaa atactcaggc aaaacttttg agagttctgc
     4261 aggaaaaaga aataatgcgt gttggaagca acaagaatat tcctgtagat gtaagggtaa
     4321 ttgcagcaac taatgcagat ttggcaaaat ccgtgaggga aggaaaattt agagaggact
     4381 tattttacag actcagcatt ctgccgataa atatacctcc tcttagagaa aggattgaag
     4441 atatcccctt attggtaaaa agttttattg caaagtttaa tgatgaatac ggaagaaata
     4501 ttgttgatat atcaaatgat gctttagatg ttttgatgtc tcatgaatgg ccgggcaacg
     4561 taagggaatt ggaaaatatt atcggccggg cgataatata tatgaatata aatgaaaggg
     4621 ttatcaggac aacacattta ccccagctga attcaaataa agttcaaaat acggttaaga
     4681 gtacagtatt aactgatgat gatgtttcaa ctttggatag ggtactgagt aaaacagaat
     4741 ctgcatatat tgaaagtgta tatttgaaat ttgacaagaa taaaacccga actgccaaag
     4801 agctgggtat atctttaagg tccttatatt ataaaatgga gaaatacggc attttttaat
     4861 ttgcaatttt ttgcaaaatg caactttctt gaaaaaaatt gcatgcaaaa aagttcaagc
     4921 tgatttaata aaaaaatcta tcgttgaaat ttcaatggtc gatttttttg tttttttggc
     4981 ataattgttg caagttatat tttgaggtgt ttgtatatga atagatttga tgagttatta
     5041 aaaatggcaa aaaccactgt gccaaaaaaa atagcaattg cagccgcaca ggatgaagat
     5101 gtgttaatgg ctgtaaaatc agcttatctt gaaaaaatct gcattccaat tttgataggc
     5161 aataaagaaa aaataattga actatcacaa aaaataaact ttaatctcga tgatattcaa
     5221 attatcgagt caaaagacga caatgaagct gctaggacgg cagtatccat ggtcagcagc
     5281 ggcaaggcag atatcctgat gaaaggatta gtcgatactg caattattct taaagcagtt
     5341 ctcgataagg aaatcggact taggacagga aatattttaa gtcatgcagc agtttttgaa
     5401 tcggacagat accataaact gttcataata actgatgcgg caatgaatat tgcaccttct
     5461 gcatctgaaa aaaaacaaat aatcgaaaat acccttccct tatgcaggtc attgaatatt
     5521 gaaaacccaa aagttgctgt aatatgtgca aaggaaaaag tatcacctaa aatgcaggct
     5581 accgttgatg ccgaaattct tgtgaatatg aacaaaaatg gtgaaataaa aggctgtatg
     5641 gtagagggac cttttggact tgacaatgca atttccaggg aagcggcagc tttaaaagga
     5701 gtaaagggag aagttgcagg tgatgcagat attttattaa tgccgaatat tgaggcagga
     5761 aatgtaatgt ataaaacatt aacatattta gctgattcaa aaaatgcagg aattatatta
     5821 ggtgctaagg ctccaatcat tcttacttca agggctgatt cagatgaagc caaactttat
     5881 tcaattttat tagcagtaat ttgttcaaaa aaggagagca tatgaaacaa ttaattatta
     5941 acccggggtc aacatcgacg aaaatagcag tatttgatga tgttaccatg atttttgaaa
     6001 aaactatacg acacacaagc gaagaattgc agccttttaa cagggttgta gaccagtttc
     6061 aattcagaaa agaaatgata atatcctcat taaaagaaaa caatatatca ttaaatgaat
     6121 tggacgctgt agtaggaaga ggaggtttac taaaaccgat tgaaggcggc acttatttgg
     6181 taaatgataa gttaataaaa gatttagaaa ccaatataaa aggtgagcat gcttccaatt
     6241 tgggagccct cttggcgcat tccatagccg atgaagtaaa tattccatca tttatagtag
     6301 accctgtggt tgttgatgaa atggaggatg tggcaaggat aagcgggcat ccggagtttg
     6361 aaagaatttg catttggcat gcattaaatc aaaaggctgt tgcaagaaaa gccgcaattg
     6421 aaaagttcgg gaaaaagtat gatgaagtga attttgtagt tgttcattta ggaggcggaa
     6481 tttctgtcgg agcacataga aagggaagag taattgatgt aaacaatgct ctaaacggcg
     6541 aaggaccttt ttcacccgaa agaacaggct cattacctgc tgcgcaatta gttaagatgt
     6601 gctttagcgg caaatatacc gaagctgaaa tatataagat tatagcaggc aagggcggaa
     6661 tttgtgcata tttgggaaca aacagtgcaa aagaagtcag cgacagagca aatgacggag
     6721 atgataaagc aagactcatt tattctgcaa tggcatacca agttgcaaag gcaatcggcg
     6781 aatatgcagt agttcttgac ggagatgttg atgcgattat aataacaggc ggtattgctt
     6841 acgacaagga ttttgtcaag atgattgaag ataaagtaaa attcatagct aaggttatag
     6901 tctatccggg agaggacgag cttttggctc tggccgaagg tggattgaga gttttaaagg
     6961 gtgttgagga ggctaaaacc tataattaag acataataaa tattccgaaa ggagggatat
     7021 tgtgcatgac aaaagattag ttattgttat aggccactac ggcagcggca agacagagtt
     7081 tgcggttaat tatgccgtta aaatgaaaga aatatacgag aatgtcagta ttgccgattt
     7141 agatatagtt aatccttatt tccgatcaag agaaaaaaga gaattttttg aagaaatcgg
     7201 gataaaatta ttcgattcaa gtattagaaa cacggcagtc gatttacctg cattgccggc
     7261 tgaactgatg ggagttataa caaacccgaa tattaaatcc ataatcgatg ttggaggcga
     7321 ccctgtggga gcaagagttc ttgccagatt cgcagaacaa ataaaaaatg tcgattatga
     7381 tatgttttat gtgattaacg gaaacagacc tgatacaagc actgttgatg gagtaatcaa
     7441 ttatcttaaa atgattgaag caacatcaag gttaaaaatt accggattag tcaacaatac
     7501 gcacatgctt aaagcaacta cagtagaaga tgtggaattc ggtcatgagc ttacaaaaaa
     7561 ggtatcatgg gaaacagata ttcccataag atatgaagct gttataaaag agacagctga
     7621 taaaataaag aaccatgaaa taatagaaaa attattcccc ataaatcttt atatgaggga
     7681 agaatggatg agttaaagga ggttttataa tggcgaaagg taaggttaca tttaatgacg
     7741 atgcttgcaa gggttgtgag ctttgtgttg cggtttgtcc ggtaaaaatt attgaattaa
     7801 ataaggaaaa aataaataat aagggttaca gtcctgcaca cgtaactgat caagaaaaat
     7861 gtatagcttg cggtaactgt gcaattatgt gtcccgattc ggtaataaca gtagaaagat
     7921 tataatggag gttaattatg tcaaaagtat taatgaaagg caatgaagct gtaggtgctg
     7981 ctgccattaa ggcgggctgc aaatgtttct tcggttatcc cataacacca tcaagtgaaa
     8041 tccctgaata tatttcaaga aagttacctg aagcaggggg agtatttctg caggcagaaa
     8101 gtgaaatagc tgcaattaat atggtttatg gagcaggagg agcgggagca agatgtatga
     8161 ctgcatcttc aagccccgga atatctttaa aacaagaagg tattacttac gctgcaggag
     8221 ctgagattcc atgtgttata gttaatatga tgagaggcgg ccccggactt ggaagcatac
     8281 agccttcaca agcagattat tatcaatcta caagaggcgg cggaaacggt gattacagaa
     8341 cattatgtta tgcaccttca agcatacagg aattagttga ttatgttatg ttagcttttg
     8401 ataaagctga ttattacaga aatcccgttt taatagctgc agacggaatg attggtcaaa
     8461 taatggaagc ggttgaattt aaggaaccgg aaccgataga attgcctcct aaagattggg
     8521 ctacaacagg aaccggcgga aaaagaaaac caaatgttgt aaactcactt tacttgcagg
     8581 cagaagaact tcaagaacac aataaaagat tagaaaaaaa atataaaata atggaagaaa
     8641 acgaagttcg ttatgaagca tttggcttag aagatgcaga ggttgtatgc gtagcatacg
     8701 gtactacatc aagaatagta aaatccgcaa ttaagcaggc acaagcaaag ggcataaagg
     8761 ttggtatgat aagaccgatt actatatggc cctatccgta tgatgtattt aaagagattg
     8821 gtcctaatgc aaaaggaata cttggcgtag aaatgagcac gggacaatta ctggatgaca
     8881 taaaaatagg tattgccggc agactgcctg tatatttcaa tggtactcaa ggcggtatgg
     8941 ttccaacacc gggcagtatc ttggcagata ttgagaaaat agcaggaggt acaaaataat
     9001 gacaattgta tatgaaaaat caaagggctt aacagataca ccttttcatt attgccccgg
     9061 ttgtacacac ggtgttattc ataaattagt tgcagaatca ttggtagaat taggagtttt
     9121 ggatgatgca atcggagtag ctcctgttgg atgttccgta ttagcttaca attatttcaa
     9181 ctgcgacatg caagaagctg ctcacggaag ggcacctgcc gttgctacag gtattaaaag
     9241 agtacatccc gacaaggttg tattctctta ccaaggtgat ggagatttag cttctatcgg
     9301 aactgcagaa attgttcatg cagcacacag aggcgagaaa attacaacta tatttgtaaa
     9361 caacgcaata tacggaatga caggcggtca gatggctcca accacactgg tgggacaaaa
     9421 aactaccaca tctccatttg gaagagatga aaatcattgc ggaaagccga taagaatagc
     9481 agaaatgttg gcaactattg atggagcaaa atttgttgaa agagttacgg tagatacccc
     9541 ggcaaatatc aggaaggcta aaaaagcaat taagagagct tttgaatgtc aaatcaaagg
     9601 cgagggtttc gcaattgtag aagtgttgtc aacatgccct acaaactggg gatttactcc
     9661 tttaaaagca ttggagtggc tgagagataa tatgattcca tattatccga taggaaattt
     9721 cagagatttt ggggagggga aataatatgg aacaaaaagt aattatggcc ggatttggcg
     9781 gtcaaggaat tatggctatc ggaaggttat tggcatatgc tggaatgctt gaagataaaa
     9841 acgtaacttg gatgccttct tacggtcccg aaatgagagg aggtacagca aattgtgcag
     9901 ttgttgtatc agaggaagaa ataggctccc ctcttatttc taaggatgga actgcagcga
     9961 tagttatgaa cttaccttca atgattaaat ttgaaaagga gcttgttccg ggcggaaaac
    10021 taatagtaaa caaatcattg attgatgttg aaccgacaag aaacgattta gatgtttatt
    10081 atattccggc taacgagtta gcattggaat taggtaacgg taaagtagca aatatggtta
    10141 tgcttggagc atatttagag attactaaag cggttgaaat tgaatcagta ttgaaagctt
    10201 ttgtaaaagt tttcggagag aataaggccg atctgatacc tttgaacaaa gaagccttac
    10261 tgaagggtgc tcaggcagca aaaaaataaa gcgtttagaa aataagtgtt ggaaatttta
    10321 atcaaagctg ttatgatata aaaatattat atgtattggg gcaacaaggt atggggacac
    10381 acctctcctc tgtagggtaa acctatgaag tcagagaaat gtccccaaat ttaaagtctg
    10441 tgagggcaga ggaatgtccc gaaataataa atataaaaat aaaggaggct tattaatgga
    10501 aaaaaaggac atcaaatggt cagaattggg atttggttat attaagacag acaaaagata
    10561 ctattcttat tggaaagacg gaaaatggga tgacggacag ctttcagagg ataataaggt
    10621 tcatataagc gaaggctcca catgtctgca ttacggacag gaatgttttg aaggattgaa
    10681 agcttattct actaaggatg gaggtattca acttttcaga cctgatgaaa atgcaaagcg
    10741 tatgcaaaaa agctgtgaga gattactgat gcccattgtt cccgtggaaa aatttatcga
    10801 tgcctgtgtg caggtcgtta aagctaacgc agcatgggtt cctccatacg ggtcaggtgc
    10861 tacattgtat ttgagaccgt atgtaatagg tataggagac aacgtaggcg taaaacctgc
    10921 agaagaatac ttatttggaa tattctgcac acctgtcggc ccatacttta aaggcggaat
    10981 gaatcccgtt aatttcatta ctaccgacca agacagagca gcaccgaacg gaacaggaaa
    11041 aattaaagta ggcggaaact atgcagcaag cttgtattca catttcgaag ctgttaaaaa
    11101 gggttttgcc gattgtattt atttagatcc ctcaacccat acaaaaattg atgaagttgg
    11161 agcagcaaac ttctttggca ttaccaagga caacaagttt gtaacacctc gttcatcttc
    11221 aatacttccg agtataacaa gaatttcttt aatgcaggtt gctaaagatt acttaaatat
    11281 ggaagcactt gaaacagata tatatgttga tgaattagat aatttgaaag aagcaggggc
    11341 ttgcggtacg gcagcggtta ttacaccgat aggcggaatt atgcataaag acaaattcca
    11401 cgtattctac agcgaaacag aggtaggacc tgttactaaa aaattatatg acacattata
    11461 tggaattcag atgggtgata tagaagcgcc tgaaggatgg atatacaaag taatgtaata
    11521 cttttctcca aatggacatt aaatttacat atttaatgtc caattttttt ttcttcaata
    11581 ttttatatat ttatgatatg ctatggtatg gaaacaatag agatgaggat attatgaaac
    11641 aaataattaa agaagcagca tataaattag gaatcgaaac agtaggtata accggcattt
    11701 cagactattc ctatttagaa gagcttttaa caaacagaaa aatcaataat cagtgctgtg
    11761 aatttgaaga gcaggatata aaaaaaagaa taaatgccaa aaatctcttt cccgaattaa
    11821 agagcataat tgctattggt attccttacg gagaaggtta caaacttcct tcatgcagaa
    11881 ataaaggttt attaagcgta tcatgtcatg gagaagatta ccataagacg gttaattctt
    11941 tattggttaa gctggctgaa gaaataatgc gtcataaaag ttttaaattt cttccctgtg
    12001 ttgacacatc atatctgata gataaagaaa tatgcagaag tgcaggtata ggaagttatg
    12061 ggaaaaacag cctgctcata aataaaaatt acggctcatt tataaattta ggctatcttt
    12121 tgactgatat tgaaacagaa acagacatta taagcgaaaa ggatatatgc ggtacatgca
    12181 atctatgtgt aaaaagctgt cctaacaagg caatatttga aatcggaggt attaatccaa
    12241 aaaaatgcat ttcttattta actcagacaa aaaactatat acctttggaa taccgagaaa
    12301 atatgggaag acaaatatat ggctgcgata tatgccaaat tgtctgccct ttaaataaag
    12361 aggttctgaa taaagaggta aatacagttt attcagacct aattgttgat ttggaagaat
    12421 tgctgaatat atcaaacaaa ggttttataa ataaatatgg acacaccgca ggcagctgga
    12481 ggggcagaaa tatatggaag agaaatgctc ttatttctat agggaatctt aatattaaat
    12541 cattgtatca aaatgttaaa gatgagcttc aaaatcagtc ggagataata aggtgctacg
    12601 cagcttggtc tttattaaaa ttaaacagac ccttggctga agagatacta tatagtaatt
    12661 taaaatacga aaatgattat gtaaaaaatg aatacttaaa actattggag gcaaaattat
    12721 gatagtttgc tcatgtgaaa ttgaaatatt gatatatgaa tctaattccc taaaggaaaa
    12781 aagacaagta attaaaagta taatagaaag aattaaatca aagttcaatg cttccgtagc
    12841 agaagtcgga tataatgaat tatggaacag gtccttgata ggaattgcag ttgtatcaaa
    12901 caataaaaga gtatgcgatg aatcaatatc aaagatagtc aaatttatag actatgatga
    12961 aagagtggaa atactaagct gcaataagga gataatataa tgaccaagac ggaaaaagtg
    13021 tacgatttgt taaaaaaagc ttatcctgat gcaaagtgcg gacttgtttt taatacccct
    13081 cttcagctgt taatttcaac aattttaagt gcacaggcta cagataaaag tgttaataag
    13141 gtaacagagg aattgtacat gcaatatcca gatttggata gtctattaac tttatctcat
    13201 gaagaaattt cactaaaaat taaacaaata ggattatata gaacaaaatc taagaatatt
    13261 tacaatatgc tgcgagtgat aaaaaacaat tataagggag aagtgcctaa aaatatggat
    13321 gatctaatgt ctctgccggg tgtcggaaga aagactgctt ccgtagtttt ggcagaagct
    13381 tttaatatac ctgcctttcc tgtagatact catgttttta gaataaccag gcgaatagga
    13441 attgcaaagg aaaataccgc agacaaagta tcggatgaaa tgatgaaaaa gctcccaaaa
    13501 tataagtggc acctcatgca ccatctgtta ataacacacg ggagaacaac atgcactgcg
    13561 aagtctccta aatgtacaat ctgcaatgta aatgaaatat gtacttatta taaaaaagta
    13621 gtaaaggaaa agtaaatttt cttgtattta ctaaaattat gccttattat aatcagggaa
    13681 tgtctattta attaataacg taggagaaaa taataaatgt cattaaatat agttttattc
    13741 gagcctgaaa taccgcaaaa tacgggtaat attgcaagaa catgcgcagc tacaggctca
    13801 aaacttcatt taataaaacc attaggtttt tcggtaaatg ataaatactt gaaacgtgca
    13861 ggacttgatt attggaattt ggtcgacatt acatattatg aaaattttga agaatttaag
    13921 aaaacaaccg gaaataataa agtatttatt tcaacaacaa aagagagcag gttttatacg
    13981 gaagtagctt attctgatga gtgttatata atttttggaa aagaaactgc aggtcttccc
    14041 ggttatattc acgaagaata tataaaaaac aggattaaaa ttccaatggt tcaaaaggtg
    14101 aatgcaaggt cattaaatct ggctaattct gttaatataa ttgtttatga agctttaaga
    14161 caattgggat ttccacagat gatataggag gaactatgga tattgctatt agtttagtag
    14221 gaggattagg gttatttctt tttggaatga tatatatggg ggatgggctc caaaaagctg
    14281 caggaaataa aatgaaggat atattggcag ctttaacaaa aaataaattg atgggcgtct
    14341 tggttggggc acttgttaca ggtgtaatcc aaagcagtac tgcaacaact gtaatggtta
    14401 tcggttttgt aaacgcaggt ttaatgaatt taaaccaggc ggtggggata ataatgggag
    14461 caaatgtcgg tactacagta acagccctat tagtatccct taatataact aaaattgcta
    14521 cttttatggc aggtgtagga gtaattgtat accttattgc aaaaaaacga aaagttaaaa
    14581 atttggctga agtaataata ggttttggca tcttgttcat aggcatggat ttaatgaaaa
    14641 atgcaatgga gccattacag tcaaacccta tatttgtaaa tttaataact aaatttgata
    14701 atccttattt aggtatcgtt gtaggttttg taatgactgc catactacaa agcagcagtg
    14761 ccacaacagg tttattgata gcggtagcag caacaggatt aatatcattt gaacaagctt
    14821 atcccattgt tttcggccag aatataggaa catgccttac agccttatta gcaagtatag
    14881 gtgccaataa aactgcaaag agagcagcat taatacattt gttgtttaat gtattaggaa
    14941 caattttgtt tacgttattt ttgagaatac ctgttcaaag gttagtatac atggcagctc
    15001 ctgtatacgt accgaggcag atagcttttg ggcatgtttt ctttaatatt ataaatgtta
    15061 ttatattgtt tcctttttcc gattttattg ttaaagcatc agagtttatt atcagaaaag
    15121 atggtcgtga aactgaaagt gtaacaaaat acattgatga aagaatattg gcaactcatt
    15181 caattgcctt gattcaaacg gcaaaggaag tactgcattt gggaaattta gttatggacc
    15241 aatttgaaac tgcaaaaaaa gcgctatttg aaaataatga ggaattagca tatgaggttt
    15301 ttgataaaga gaagaaaatt aacagattgc acaaggaaat attagaatat cttgttaaac
    15361 ttgataaggt ttcattaaca agtgccgaaa aggataaatt gttcgttttg atgaatgcaa
    15421 ccagtgatat tgaaagagta ggagatcata tagataatat tggagaattg ttgttatata
    15481 aatttgagaa caaagcagac ttttcggaag aagctactaa tgaaatagct aatatgttta
    15541 agattactat ggaagcatat aagttatccc tggattcatt ggcaagtgct gaatcggatg
    15601 tttgcagggc ggttgttaaa tatgaagatg atattgacaa aatgtataaa acactaagga
    15661 agaatcacat agacagactt aataacaata tttgcaatcc aaatgcagga atagtatttt
    15721 tggatatgat aagcaatttg gaaaggattg gagaccactc attaaatata gcagaataca
    15781 ttcttgaggt aatataaata agggcagata tcgcttaatt taggctgtaa gaagtactaa
    15841 atatttgaaa atatttgtaa aaaatagttg ctttatataa gaaaatttta tatactaata
    15901 ctagtgataa ttgaataatt gaataattga atgaaattcg cgggagagat tgttatctaa
    15961 ttaacaacgc cgaaggacat aagttacaaa ttagccgttt gtaatgacta tctcaggcaa
    16021 aaggaccgtg aattgacaaa tctctggaaa gcgcaagcac cgaaggggca attccaaagg
    16081 aagaaactct caggtataac aacagggcag gaggcaggat atatttgatt ttgctttctg
    16141 cccttatttt taacaggtga aaaaaatgat tattacgaac aatgattggg tattaaataa
    16201 atacaaagac atctataatg ttgtttatat cgacggtaca tttaaagatg ttttgattgc
    16261 agttagagac aaaatacata ttggccatga acttttgaca catcccttag gcagcagcgt
    16321 taaaccaagc gaaacccctt ataaatctgt aattgtttca gacaacaaaa attatacaag
    16381 ctttgattca atttatatga ttgaaaatgc aataattaca tatgataagt ttaataaaga
    16441 taaattaaaa ttaatcagcg ataaagtaaa agaagattta aaattgattg atttaacagt
    16501 gctggaaagc gcgttagata tataactctt taaaaaacta atggaggtga agattttgaa
    16561 attagaatta ggctatattt ttattaagga tattcagttt tctgatgtat ccaaggtaga
    16621 aaacggtacg ttgtacgtta acaaggaaga agtaaaagca ttaattctag aagatcaaaa
    16681 tttcaaaaca gcagatgtag aactggcaaa gccgggagaa agtgttcgca taatgcctgt
    16741 taaagatgtt attgagccaa gagttaaggt tgaaggcccc ggaggcatat tcccgggaat
    16801 ggttagcaaa gtagatactg taggctcagg caaaacaaat gttttgaagg gtgcagctgt
    16861 tgtaactaca ggcaaaatag taggtttcca agaaggtatc attgatatga gcggaacggg
    16921 agccgaatat acaccttttt ccaagcttaa caacttagtt ataatttgcg agcctattga
    16981 cggattaaag cagcacgagc atgaaaaagc cctaagattt gcaggataca aggtagcatt
    17041 atatcttgga gctcttgcaa aggatttaac tcctgatgaa gttgaagtat ttgaaacacc
    17101 taatttggtt gaagggataa aaatgtatcc cgaactacca agagtagctt atgtattcat
    17161 gctgcaaagt caaggtcttt tgcatgatac ttatgtttat ggagttgatg caaaacaaac
    17221 attaacaacc atgatttatc ctaccgaagt tatggatggt gccatagtaa gcggtaactg
    17281 tgtttctgca tgcgataaga atactacata tcatcattta aacaatccta tcatatatga
    17341 tttgtttaaa gaacatggca agaccttgaa ttttgtcgga gtaatcataa ctaacgaaaa
    17401 tgtttatctt gccgataagg aaagatcatc caactggagt gcaaaacttg cgggatttct
    17461 tggtgttgac ggtgttattg tatcagaaga aggatttgga aatcctgata cggacttgat
    17521 aatgaactgt aagaaaattg aagctaaagg tattaaaaca gttcttgtta cagatgaata
    17581 tgcaggcaga gacggtactt ctcagtcatt ggcagatgca gatgtatcag ctaatgcagt
    17641 ggtttccgca ggaaatgcaa atgaagtaat tgagctgcct cctatggaaa aaataatcgg
    17701 tcatgttgag gttgctgatg taatagccgg aggatttgat ggaagcttgc ataaagatgg
    17761 aagcataaca gcggaattgc aggttattac aggtgctact aatgaaatgg gcttcaataa
    17821 acttagtgca agataggaag gaggagaaaa taatgctaaa agtagttcat tatattaatc
    17881 aattctttgc caatgtaggc ggagaagaaa tggctcacat tcctgctgaa gtaagagaag
    17941 gtgtagtagg accgggtctt gcatttcaac aagcatttaa ggatgaagca gaaattgtag
    18001 caacaattat atgcggagac tcatatttca acgagaatat ggatgaagcc aaaaatacca
    18061 taattgaaat ggtcaagaaa tacaacccgg atttatttat tgcaggaccg gcatttaatg
    18121 ctggcagata cggtgtagct tgcggcgcta tttcaaagac agttaaggat gaattgaata
    18181 tccctgttat aacgggaatg tatgtggaaa atcccggagc agatatgttt aaaaacgaca
    18241 tctatatagt atccacaaag aattctgctg caggaatgag agatgctgtt aagaaaatgg
    18301 ctccattagg acttaaactt gcaaagggag aaaaaatcgg cgcatcaata gaagaaggct
    18361 acataccaag cggtaaaaga gttaatttct ttgaaaaaga aagaggctca acaagagctg
    18421 ttaaaatgct tcttgcaaaa ttaaacaaca aagaatttga aacagaattt ccaatgcctg
    18481 attttgacag agtacctcca aatccggcta taaaagatat tacaaaagca aaaattgcat
    18541 tggttacttc cggaggaatt gttccgaaag gaaatcctga ccatattgaa tcatcaagtg
    18601 cttctaaata cggtaaatat gatatcgaag gagttatgga tttaaccgaa gaaacatatc
    18661 agacagctca cggcggatat gaccctgttt atgcaaattt agaccctgat agagtattgc
    18721 ctgttgatat tatgagagaa tttgaaaagg aaggcaaaat aggaagtctt cacagatatt
    18781 tctataccac ggttggtaac ggaactgccg taaagagtgc taaaggtttt gccgaaaaat
    18841 atgtaaaaga acttatagct gacggagttg atgcagttat actcacatct acctgaggta
    18901 cctgtacacg ttgcggtgca acgatggtaa aagaaatcga aagagcggga attcctgtgg
    18961 tacatatgtg tactatagtt cctatttcat taactgttgg agctaacaga attgttccga
    19021 caatagcaat acctcacccc ttggggaatc cttcattgtc tatagaagaa gaaaaagaaa
    19081 taagaagaaa attgctgaca agagcattaa aagctttaga aactgaagtt acagagcaaa
    19141 ctgtattcga aaagtaaaca gatgacccaa atctttgatt tgtagtcagt cgcttatcct
    19201 gatctaaagt gaaggatctc agcaatatgc gtgagattct tcacttagct cataattat
//
LOCUS       DAOSHP010000029        19066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2332_length_19066_cov_8.600915, whole genome shotgun sequence.
ACCESSION   DAOSHP010000029 DAOSHP010000000
VERSION     DAOSHP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19066
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2332_length_19066_cov_8.600915"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLB33_04485"
     CDS             complement(<1..221)
                     /locus_tag="PLB33_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62813.1"
                     /translation="MTFYNYLLSSLPNEFTAKIIYYILITIALLLACSIINFLVNRVV
                     LKYITKYIKNNKYKWDDVLLDAKLFQRIA"
     gene            complement(290..772)
                     /locus_tag="PLB33_04490"
     CDS             complement(290..772)
                     /locus_tag="PLB33_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008795068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62814.1"
                     /translation="MDVTIKPAYNEKEEILKLFKEYTDSILAEGEEVAACLKSQNYDK
                     EIIEIEEKYGLPGGRLYIACIGSETVGCVALKKIDDKYCEMKRLYVRPGNRGKHIGRK
                     LMEQVIFDAQGIGYKHIRLDTFPFMNVAIKMYKNYGFYEIERYNDNPAPTAVFMQLDL
                     "
     gene            complement(797..1213)
                     /locus_tag="PLB33_04495"
     CDS             complement(797..1213)
                     /locus_tag="PLB33_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOG62815.1"
                     /translation="MKSYRKILTFNTKTRRAFINITPQIEECLKESGIKEGLLLCNPM
                     HITSSVFINDDEPGLHRDFEAFLEKLAPEKPYSQYLHNGYEDNADAHIKRTIMGREVV
                     VAITEEKLDFGTWEQIFYGEFDGKRAKRVLVKIIGE"
     gene            complement(1361..1963)
                     /locus_tag="PLB33_04500"
     CDS             complement(1361..1963)
                     /locus_tag="PLB33_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOG62816.1"
                     /translation="MQYYKYGKKETDYLKERDSKLGREIDRIGIVKREVNPDIFDSLI
                     ASIISQQISTKAAFTVKNRLKESVGEITPETISKLDIESIQLCGMTMRKAKYIKNITQ
                     AVVSKTINLDNLNLLSDEEIINELIKFEGVGKWTAEMLLIHSFQRPDILSYNDLGIQR
                     GLKKLYSLEDISKEEFETYRKRYSPYNTVASLYLWKISED"
     gene            complement(2020..2511)
                     /locus_tag="PLB33_04505"
     CDS             complement(2020..2511)
                     /locus_tag="PLB33_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOG62817.1"
                     /translation="MIRSAEQELYNILEQLNIKYKRYEHKPVYTVEEADNLDIDIPGQ
                     HCKNLFLRNKKGNIHYLVIADSQKHIDLKLLSKEIGSSGLSFASEERLYKYLGLKAGS
                     VSPFGLINDIERKVIVLIDRKLTDKSIVNFHPNVNTATIGVSYDDLEKFIKWHENRFI
                     YID"
     gene            complement(2671..3435)
                     /locus_tag="PLB33_04510"
     CDS             complement(2671..3435)
                     /locus_tag="PLB33_04510"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018782910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOG62818.1"
                     /translation="MKLKGKTAVVTGASSGMGREIAYYFAKEGANVVAVARRAERLNE
                     LVEQTKDFEGSVIAYSADITNSQKVNEMIDEAIKQFGRMDILVNNAGIMDDMSAVGDV
                     NDEMFDKVFNLNVKTILYSMRKAVKHFEEIGGGVIINISSIAGLYGGAAGATYTASKH
                     AVVGLTKNTAFMYALKNIRCNAICPGAVATEIGNSEFMKNINMEGVQRTGIGMPLNPR
                     TGEPSEIAKVAVFLASDDASFVNGQCIAVDGGWRAY"
     gene            3602..4720
                     /locus_tag="PLB33_04515"
     CDS             3602..4720
                     /locus_tag="PLB33_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG62819.1"
                     /translation="MKNINVGMIGFGYMGKMHTMCYDIMKYYYNTDVNVNMYAVSKTS
                     KTSFPIKFEKIYPDYKSLINDENVDVIDICVPNFLHKEILIEAIKAGKHIYCEKPLAA
                     DIESANEVMEAYKKYNYNKTSRITFEYRFVPAIMRAKQIIDEGKIGNIIHFNFKYYGS
                     EFLDPNRPISWQSTKEKSGGGVLYALGTHAIDLIHYLIGDIDEVYADKRTHFKKRPLL
                     GTDKTVDVEIEDILNVQLYSGDKMGTLLLSQVAAGSGIDLTFEIYGEKGAIKFTHDNP
                     NVIYYFNNEDEKSPIGGLSGFKAIETTQKYGKAASFPPPRVNIAWTRYHIASIFDFID
                     AVANNTKAYPDIQDGYKVQLVTDAIYKSAEIKKSITVK"
     gene            complement(4853..6691)
                     /locus_tag="PLB33_04520"
     CDS             complement(4853..6691)
                     /locus_tag="PLB33_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOG62820.1"
                     /translation="MKITKLISLLLALLMITSLAACTQPAAETPPEEPPAEETPPAVE
                     EGIKSGTYEVEGVGFGGKMSVKVTIKDGKIDDIEIGKNYETNGAGRVALEYVSQRIIE
                     GQTTNVEAVSGATISSSSLMALVRKALEEAGATKDQFSEEYKIARDIKTEREAEIVII
                     GAGGTGLAAAVTAGQEGASVIVLETNGFAGGNLLVSGGVYNSPEPEMQAKQGIEDSPE
                     LFMQQTLAGGDNIANPELVRILAYNALDGLNWLKELGNEFEDQVIQAPGALHPRSHNT
                     TAPLGSGIIKTYLEHIDKMDNVEILYETKGESLIQEDGRVVGVIAKNPDGSKLTVKAN
                     KGVIIATGGFSKNAQLVMEYRNAEKWPLLDENSVSTNMDSIRGEGIIMAREIGADVVD
                     MDQMQFLYLGQPKTGLLSGVFNVSAEVTVFVNKEGKRFVAEDARRDVISSAVFAQEDG
                     MMYMLHSAESLSDPANQLNIDGIPMKELLDIGAYGWKQGDTLEELAKEIGVPAENLVQ
                     TIAEYNEAVDTKNDPFGRTLLNVKMEKGPFYAIPRVPALHHTMGGLRIDTLARVLDTK
                     GEPIPGLYAGGEVTGGIHGGNRLGGNAVTDTVVFGRLAAQSAVAGK"
     gene            complement(6937..8874)
                     /locus_tag="PLB33_04525"
     CDS             complement(6937..8874)
                     /locus_tag="PLB33_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOG62821.1"
                     /translation="MLKEDDFPFLSARSYESTLNKIESAWENFIAGEIIPDFNLPRNP
                     ILESWKRSKEMGVPYNLKCAPLKLNEHDIMVERERQHNLFMIIDSYLDSIFKIFHDEN
                     MAVSFSNEKGIILSIRAGKSMLGNCHGINYLLGAGWSENDCGTNSIGTSLYTGKPIQI
                     YATEHYCKMAHSINCSSAIIKEPSSEIVLGALTITTHKNIVPAHTLNWAISEAQKIEN
                     AIEYHLQEESTSLMNCLFEMNEQPCIICKLDGKISKINKSAQMLGVKVGDRIENSFVF
                     PKYSRFDELFDNTYKTICCKTEQRYIVTIIPWRIGNNIIGAIAYFQKESLFLNQGNSA
                     KLTSDRSEFKCIIGKSPAFRQVIDLADKFSDSDMPILITGETGTGKEVLARAIHNNSC
                     RKEKIFLPVNCGAIPKELISSELFGYVEGAFTGTKKGGRIGKIEASSGGTLFLDEICE
                     MPIDMQIYLLRVLEEGTITRVGSNEAIPVNLRIICATNKDIRHEVEIGNFRKDLYYRL
                     NPVELHLPTLRDRGDDIILLAEHFLKLNSQDFKLSADAAELLMNYSWPGNVRELKSVI
                     NRAILLSKNSIITSNELFIHDKKQEIKYSKTHTEINEKRPTSDLIKQVIEECGGNKSL
                     AARRLKISRMTLYRRIKEYNL"
     gene            complement(9035..10006)
                     /locus_tag="PLB33_04530"
     CDS             complement(9035..10006)
                     /locus_tag="PLB33_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOG62822.1"
                     /translation="MYYMGVDVGSVSTDIALIDNNYELIEKIYLRTKGNPIKAVQEGF
                     KILKNKYEDSEIYAVGTTGSGRMIASAIIGADVVKNEITAHATAALEIDKDVKTIIEI
                     GGQDSKIIIINNGIVVDFAMNTVCAAGTGSFLDRQAERLEIPIEEFGDYAMKASTGVR
                     IAGRCTVFAESDMIHKQQLGYNHAEIIRGLCDALVRNYLNNIGKGKTILPKVLFQGGV
                     AANKGMKKAFENELGFEIYVPENFSMMGAIGAAIIAANKVKKTGKTNFRGLKLADNNI
                     FSRSFDCEGCPNKCEVVKIYENDDIIGYFGDRCTKWSSKICEKYKLA"
     gene            complement(9987..11084)
                     /locus_tag="PLB33_04535"
     CDS             complement(9987..11084)
                     /locus_tag="PLB33_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOG62823.1"
                     /translation="MKATFPHMGNIYIAVKALFDGLDIDYVIPPQNSTEALNIGSKYS
                     PEEICLPFKIMIGNYIQAIERGADTVILTGSCGPCRFGEYCEIQMNLMKNLGYDLNFI
                     VLDMPKDIGLKELLKRLVKVGSTSKKSSFEKLKAAKNAIEIMNLIDETESLAHYLAGY
                     ERNKGECKRLLHKCLKEAVKLSNPYEMLSLIKEYRNKLKYIPVDINKDPLKIALIGEI
                     YTVIEPYSNFYIEDKLMDYGVSSKRGMTPSWWLKDMALKPSKLNSLKIKKSSREYLSM
                     EIGGHARECIGEAILAYKEGFDGAIQIFPMGCMPEIVSKSILPTISKDYGFPIMSLVV
                     DDMTGEAGYITRVEAFIDLLERRRDNVLYGS"
     gene            complement(11056..11976)
                     /locus_tag="PLB33_04540"
     CDS             complement(11056..11976)
                     /locus_tag="PLB33_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOG62824.1"
                     /translation="MKVGIPKGLMYCKYHPFLVTFFSELGAEIIVSDDTNKNILDAGV
                     KYCVDDACLPVKIFHGHVDSIKDKCDVLVIPRIMQLWKNEYICPKFCGLPEMVINSFD
                     KLPVTITEPIYATTKKKLYNWAKSSGKFLRKNNYEIKRAFINALNAQKAHEIGIYDSG
                     YEINIALTGHPYNIYDSFLNMNLVKKINALGMGIITAEFTGEDILQKESENLYKRPFW
                     TFVRENYGFAVNAAKEKNVDGIIYISSFNCGTDSVIVELIKDGVPDFPFLILKIDEHT
                     GEAGINTRIEAFRDMLERRLFNESNISTHG"
     gene            complement(12080..13063)
                     /locus_tag="PLB33_04545"
     CDS             complement(12080..13063)
                     /locus_tag="PLB33_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOG62825.1"
                     /translation="MFNSELMKRLSDCFGPSGREKRIREMIMDEIKDFADEITIDPLG
                     NLIARKKGTGKKILFSAHMDQIGLIITHVDEKGLLRFANVGGLNAKELLGLRMVFDNG
                     LEGVICQEELKDNEKLSTSKLFLDVASTDKEFIAKNFRVGDMCVFKSEYYETEDCVIC
                     KAADDRIGCYVLIEALKNHPETNNDVYYVFSVQEEVGCRGAKTAGYNINPDLAIAVDV
                     TATGDTPDGIKMDVKLGNGAAIKLMDKSIITHTEVKDLLTDIAEKNSIKYQYEILEFG
                     GTDAGPIHTSRDGVPSGVISIPTRNLHTSGEIFNKFDVEECIKLVIGTAAK"
     gene            complement(13065..14078)
                     /locus_tag="PLB33_04550"
     CDS             complement(13065..14078)
                     /locus_tag="PLB33_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOG62826.1"
                     /translation="MFLNELTKLSGVSGCEFEVRNYIKSKLSEMGCQFYVDKLGNIIA
                     HNKGKKNKTIMVSAHMDEVGLIVTNIDSDGFIKFKAVGGIDQRVLNSKVVLIGENNIP
                     GVIGSKAVHLMTEKEKGVSLPIDKLYIDIGTSTKEETEKLVNIGDYAAFKSDYVEFGD
                     NMIKAKALDNRVGCSIILEILKEKLDVDFYGVFTVMEEVGLRGAETAAYQLEPDLAIV
                     LEGTVCADMPEVEEHENSTLIGKGAVLSLMDNATVYDIDLVRRVAKIAEDNNIAYQYR
                     RSTSGANDAGVIHKTKKGAKVVSISVPCRYIHSSVSVASKNDLMSALKLTKQVIIENQ
                     KGE"
     gene            complement(14069..15109)
                     /locus_tag="PLB33_04555"
     CDS             complement(14069..15109)
                     /locus_tag="PLB33_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOG62827.1"
                     /translation="MLKEFLFDICSNQFVSGFEHINGDILIKYFKPYTDSFEKDRLGS
                     YIFKSSGSKDTKIMLAAHIDEIGLMITGILDGGFLSFITVGGFNPASLIAQDVTIFGK
                     EKIFGVIGIKPPHLTSEEERKKPLKTRELYIDTGYTKEKLEKLITIGDIATINREPLS
                     LQGSVISAKAFDDRACVAVMLETAKELKKISHKSNVYFTATAQEETGLRGAKTSSYKI
                     APDIGIVLDVGFGKTPDMPSDSALLGKGPIVAYGGRLNTKLTKKFVEICKKYNYPMQY
                     EAIPVRTGTDTDALQISRDGIPCILLSIPLRYMHTSVETIDLKDLEITGKAIARFINE
                     LDNSDLEEILCF"
     gene            complement(15164..17578)
                     /locus_tag="PLB33_04560"
     CDS             complement(15164..17578)
                     /locus_tag="PLB33_04560"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOG62828.1"
                     /translation="MKIIVSEYNGFCGGVKAAVLKADKILTDEKKLYCYGEIIHNKDV
                     VEKLKNKGMVIVDDIPENKDAKFLIRSHGVGKNIYDRLEEKNIEVIDATCVKVKKIHK
                     IVEEFKNKDYNIIIVGDENHPEVQGISGWCNYEAVIIKSPEELKNIIEPTKKYCLVCQ
                     TTFNLNTFEEILKTIEINNYSNIIVHNTICDATKKRQEACIALASKCDIMLIVGGKNS
                     SNTKKLYELSKELCENTFLIQNYKEIPYNYIDKNSIVGVSAGASAPDWIIEEVINMLE
                     NLNNNMNEMNEQAEHADLAEQSEPAELSDKKEQTELVEQSEETEKTETVEQTDKAEKV
                     NSMHELLENYGGVVNLGIGEKVKGTVIYVSPDAVSVNINYKSDGIITREDFSLEEVSD
                     LTEVVKEGDEIDAIVVKISDQDGNVVLTRKPLEEQKIWDDLEQMRIRGEIVEIPIIEA
                     SQHGIYGKLKGIKGFIPRNQISIKRNVDPSEYVGKNLKVKILETKNKKGRRQLVLTSR
                     AVEKFEKEIKDNEVWETIQEGEIYEGTVKNLQDYGAFVEINGIDGLLHINEIAWTRIK
                     HPSEVLKPGDKINVKVKNVDKENKRLSLSYKATVKSPWAKFLDKYQKGDIVTGVVTRI
                     VDFGAFVKVDDIECLLHIKDLDWARTEKVTDILQEGQEVTAKILSITRKDRKVGLGLK
                     QLTEHPFDLYAKTLNKDDIIQVEVTRILLDGIHVNANGNIDCFIPIAKVSTEKLRTPA
                     QVVKVGEIVDAKVQSIDMKGKNLNLTLILDDKSEETVENKSYQSEDTNFTIGESIGDA
                     LDDLLK"
     gene            complement(17575..18171)
                     /locus_tag="PLB33_04565"
     CDS             complement(17575..18171)
                     /locus_tag="PLB33_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOG62829.1"
                     /translation="MFYKIAVIVLKVLVFIIFDLEVYNRENLDNIDGGLIICGNHRSM
                     IDPVILAVSTKRQIHFMGKKELFENKFLSFLFRSLGAFPVDRKGVSLSAIKNSLNVLN
                     KGGILGIYPEGTRIKTGFDEKNAKSGIALIANKAKVGIMPVYLDGPYKFRGKLRLYIG
                     EEKDYFKNYDGRLNSEDYTEIGIEILKDIYRLKNEGMK"
     gene            complement(18161..18811)
                     /gene="cmk"
                     /locus_tag="PLB33_04570"
     CDS             complement(18161..18811)
                     /gene="cmk"
                     /locus_tag="PLB33_04570"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOG62830.1"
                     /translation="MIIAIDGPSGAGKSTVARLLSKELGYEYIDTGAMYRALAYKAYK
                     QNIDINEANIAELLETTNITYNDNKVFLDGENVENLIRNEEISTAASKISSLKIVREK
                     MVEIQRKIAKNKNVVLEGRDIGTIVFPDAEHKFFITASLEERAKRRYEQLKLNNIKAD
                     YTNVINDMIKRDENDSTRKFSPLKPAEDAILIDTTNMDLNEVTKTIAQYIGGSNVL"
BASE COUNT         5410 a         3811 c         2734 g         7111 t
ORIGIN      
        1 aaggcaattc tttgaaataa tttagcatcc aacaatacat catcccattt atatttgttg
       61 ttttttatat attttgttat atattttaag actacacggt ttacaagaaa atttataatg
      121 gagcaagcta ataataaagc tatggtaatt aatatgtaat atataatttt ggctgtaaac
      181 tcattaggca gactgcttaa taaataatta taaaaagtca tatatctctc ctatttaaaa
      241 attaatgtgt attagtacat ttattgatta aacatagttc tagtacattc tataaatcta
      301 attgcataaa tactgctgtc ggtgcgggat tatcattata tctttcaatt tcgtaaaagc
      361 cgtaattctt atacatttta attgcgacat tcataaaagg aaatgtatca agtcttatat
      421 gtttatatcc gattccctgt gcatcaaaaa taacctgttc cataagtttc cttccaatat
      481 gttttccacg attacccggt ctgacatata agcgtttcat ttcacagtat ttatcatcaa
      541 ttttttttaa tgcaacacac cctacagttt cactgccaat gcatgctata tataacctgc
      601 ctccgggcaa gccgtatttt tcttcaatct caattatttc tttatcatag ttctgtgatt
      661 tcaaacatgc ggcaacctct tcgccttctg ctaatatgct atctgtatat tctttaaata
      721 atttaagaat ttcttctttt tcattatatg ccggcttaat tgttacatcc atattaagta
      781 cctcttgata ttttagctat tcaccaataa tttttaccaa tactcttttt gctctttttc
      841 cgtcaaattc accatagaat atctgttccc acgtaccaaa atctaacttc tcttctgtta
      901 tggctactac aacttctctt cccataattg ttctctttat atgagcatcg gcattatcct
      961 cgtatccatt atgaagatat tggctatatg gtttttccgg agcaagtttt tccaggaaag
     1021 cttcaaaatc cctatgcaag ccgggctcat catcatttat aaaaacactg gacgttatgt
     1081 gcattggatt gcataggagc agtccctcct taatgccgct ttcttttaaa cattcctcta
     1141 tttgcggagt aatattaata aatgccctac gagtcttggt gttaaaagtt aatatttttc
     1201 tgtaagattt catatattct ccttatccaa tttttgttcg tattatcctc tattatagat
     1261 tatttatcgt taaaattcaa ccaaatatgt agcggctgcg gtttacataa tataaattat
     1321 gtaaactcat tttgctatat aataaggaag ctagtattta tcagtcttct gatattttcc
     1381 ataaatataa agatgctacc gtattgtaag gggaatatct ttttctgtac gtttcaaatt
     1441 cttctttgga aatatcttct aatgaataca atttttttaa tcctctttgg atacctaaat
     1501 cgttataact taatatatcg ggtctttgaa aagaatgtat aagcagcatc tctgccgtcc
     1561 atttccctac cccttcaaat ttgattaatt catttatgat ttcttcatca cttaataaat
     1621 ttaaattatc aaggtttata gtcttgctta caactgcttg agttatattt ttaatatatt
     1681 tggcttttct cattgtcatc ccgcatagct ggatgctttc gatatctaat ttgctgatag
     1741 tttccggagt tatctctcca actgactctt ttaatcgatt ttttaccgta aaagcagctt
     1801 tggtagatat ctgttgactt ataatacttg ctataagcga atcaaatata tccgggttta
     1861 cttctctttt tacgattccg attctgtcta tttctcttcc taatttggaa tccctctctt
     1921 ttagataatc tgtttctttt ttcccatatt tgtaatattg catgttaatc tcccatatgc
     1981 tataactcaa tgttgaatat cggtataaat tctattgact caatctatat aaataaatct
     2041 gttttcatgc cattttataa atttttccag gtcgtcataa gaaacaccaa tagttgcagt
     2101 atttacattc ggatggaaat ttacaatact tttatcggtt agttttctat cgattaaaac
     2161 tattactttt ctttctatat catttattaa accaaaagga cttactgagc cagctttaag
     2221 tcccaaatat ttatataatc tttcttcgga tgcaaaggat agacctgaag accctatttc
     2281 tttcgataat aatttcaaat ctatatgttt ttgtgaatct gcaataacaa gataatgaat
     2341 attccctttt ttatttctta aaaataaatt tttgcagtgt tgtcccggaa tatcaatatc
     2401 caaattgtct gcttcttcga cagtatatac aggtttatgc tcatatcttt tatatttaat
     2461 atttagttgt tctaatatat tatataactc ttgttcagct gacctaatca ttttcaatcc
     2521 cttccaaata tatatttcat ctgtttttct taaactataa taacatatat taaaataaaa
     2581 gcaatattca agaaatattt gaatattact tttattataa tgatatacat tttcatctgt
     2641 aattttttgc agcaatcttg tcaaataaat ttagtaagcc ctccagccgc catcaacggc
     2701 aatacattgt ccatttacaa acgatgcatc atcgctggct aaaaacactg cgacttttgc
     2761 aatttcactt ggttcacctg ttcgaggatt aagcggcatc cctattcccg ttcgttgtac
     2821 accttccata ttaatattct tcataaattc agaattgcct atttccgtcg ctacggctcc
     2881 gggacatatt gcgttgcacc ttatattctt caaagcatac ataaatgccg tatttttggt
     2941 taaacctacc actgcatgtt tgcttgccgt atatgtagct cctgcagcac caccgtacaa
     3001 tccggcaata gaggaaatat taattattac tcctccgcca atttcttcaa aatgttttac
     3061 agcctttctc atagaatata atatggtttt tacatttaaa ttaaatacct tatcaaacat
     3121 ttcatcattt acatcgccaa ccgcactcat atcatccata atgcctgcat tgttaaccag
     3181 aatatccatt ctgccaaact gtttaattgc ctcatcaatc atctcattta ctttttgact
     3241 atttgttata tctgcagaat atgctataac gctaccttca aaatctttag tttgttcaac
     3301 cagttcattc aacctttcgg cacgacgtgc tactgctaca acattagcac cttccttggc
     3361 aaaataatat gcaatttctc ttcccattcc tgaacttgcg cctgttacga ctgcagtctt
     3421 cccttttaac ttcataaaac ctccttatat tttaatattg agatacatta tttataccct
     3481 gctaatataa tccgaaacta ttatttttca attagatgaa tcccttgatt atttctgcaa
     3541 tcgtttgtat aatattttta aagcgtgcgt tgaaatttat aactatatgg aggagaaaag
     3601 gatgaaaaat attaatgtag ggatgattgg atttggttac atgggaaaga tgcacacaat
     3661 gtgctatgat attatgaaat attattataa tactgacgtg aatgtaaata tgtatgctgt
     3721 ttcaaagaca agtaaaacaa gttttcccat aaaattcgaa aagatttatc cggattacaa
     3781 gagcctaata aatgatgaaa atgtagatgt tatagatatt tgtgtaccga attttttaca
     3841 taaagaaatt ttgattgaag caattaaggc cggtaagcat atatattgcg aaaaaccttt
     3901 agccgctgat attgaatcag caaatgaggt aatggaggca tataaaaaat ataattataa
     3961 caaaacaagc agaattactt ttgagtatag attcgtccct gccataatga gggcaaaaca
     4021 gataatagat gaaggtaaaa taggcaatat aatacacttt aattttaagt attacggaag
     4081 tgaattcctg gaccccaaca gacctatcag ctggcagtca acaaaagaaa aatccggcgg
     4141 cggagtttta tacgcattag gaactcatgc aattgattta atccactacc tgataggaga
     4201 tattgacgaa gtatatgctg ataaaagaac ccatttcaag aaaagacctt tattgggaac
     4261 ggacaaaacg gtagatgtag aaatagaaga tatacttaat gttcagcttt atagcggaga
     4321 taaaatggga acactgcttc tttctcaagt agcagcaggc tcaggaatag atttaacatt
     4381 tgaaatatac ggagaaaaag gagccattaa atttactcat gataatccta atgtcatata
     4441 ctattttaat aatgaagatg aaaaatcccc tataggcggt ctaagcggtt ttaaggctat
     4501 agaaacaact caaaaatatg gcaaagctgc ttctttcccg cctccaagag taaatatcgc
     4561 atggacaaga taccatatag caagcatatt tgattttatt gatgccgttg ccaataatac
     4621 aaaagcatat cctgatatac aagatggata taaggtacag ctggtaaccg atgctatata
     4681 taaatcagct gaaataaaga aatcaataac agttaaataa aaagttactc ttcaatatta
     4741 atataatatt agaatagacc cgggttaatc ccgggtctgt tcttaaataa gattatctac
     4801 agatctattg aatctgtata agtggaggat attatgtgaa acttttttta tctcattttc
     4861 cggcaactgc tgattgtgct gcaagacgac caaatacaac cgtatcagtt actgcattac
     4921 cgcctaatct attgccgccg tggattccac cggttacttc accgccggca tataatcccg
     4981 ggataggttc tcccttagta tctaatacac gagccaaggt atcgatgcgc aatccgccca
     5041 tagtgtggtg cagagcgggc actctcggaa ttgcatagaa gggtcctttc tccattttta
     5101 catttaataa tgttcttccg aaaggatcat tcttagtatc aactgcttcg ttgtattctg
     5161 ctattgtttg tacaagattt tcagcaggta ctcctatttc cttagcaagt tcctccaatg
     5221 tatcgccttg tttccagcca taagctccga tatccaacaa ttccttcatc ggaataccgt
     5281 caatattcag ttgatttgcc gggtcgctta atgattctgc actatgaagc atatacatca
     5341 ttccgtcttc ttgggcgaat acggccgaac tgattacatc tcgacgagca tcttctgcta
     5401 caaatctctt gccttcttta ttaacgaaaa cagtcacttc tgctgaaaca ttaaatacgc
     5461 ctgataatag ccccgtcttt ggctgtccta agtataaaaa ctgcatttga tccatatcaa
     5521 caacgtcagc tccgatttct cttgccatga taattccttc acctcggata ctgtccatat
     5581 ttgttgatac agaattttca tccagtaatg gccatttttc cgcatttcga tattccataa
     5641 ccaattgagc attcttagaa aaaccacctg ttgcaattat aacaccttta tttgctttta
     5701 ccgtcaattt agagccatcc ggattttttg caataacgcc gacaactcgg ccgtcttctt
     5761 gaattaagct ttctccttta gtctcatata gaatttctac attatccatc ttatcaatat
     5821 gttccaggta ggttttaatt atacctgatc ctaacggtgc agtcgtattg tgtgaacgag
     5881 gatgcaaagc acccggcgct tgtattacct gatcttcaaa ttcgttgcct aattctttta
     5941 gccagtttaa cccatcaaga gcattatatg ctaatatcct tacaagttcc ggatttgcaa
     6001 tgttatcccc tcctgctaaa gtttgctgca taaatagttc cggtgagtct tctatacctt
     6061 gttttgcttg catttcaggt tcaggcgaat tatatacacc tccgctgact aagagattgc
     6121 cgcctgcgaa tccgttagtt tcaagaacta taaccgaggc accttcttga cctgcagtaa
     6181 cggcagcagc caaaccggtt ccgcctgctc caataataac tatttcagcc tcacgctccg
     6241 ttttaatgtc acgagctatt ttatattctt ctgaaaattg gtcttttgta gctcctgctt
     6301 cttccaatgc ttttctaacc aatgccatca aacttgatga agaaatggta gcccctgata
     6361 ctgcttctac attggtggtt tgaccttcaa taatacgttg agaaacatat tccaatgcaa
     6421 ctcttccggc accatttgtt tcgtaatttt tcccaatttc aatgtcatca atcttgccat
     6481 ccttgatagt taccttaaca ctcattttgc cgccaaatcc gacaccttca acttcataag
     6541 tgcctgattt tataccttct tctactgcag gcggagtttc ttccgcgggt ggttcttccg
     6601 gcggagtttc tgccgcaggc tgtgtacatg ctgctaatga agtaatcatt agtaaagcca
     6661 gcaataatga aatcaatttt gttattttca tgcttccctc catttagata ataattaata
     6721 atgtttactg atgttgttta tgaaaattta aacaaacatt ctattaataa ttagcatttt
     6781 ttatgccaag aaacatgtgt taattgttgc ggtttttcat tgtattggtg tcacttttta
     6841 tgtaacattg taacatctgt cacttggtaa tatgtaacat taataaaaaa gttgcctgtt
     6901 aggcaactat acttatgacc aagcatcaca tagtcattat aaattatatt ctttaattct
     6961 tctgtataat gtcattcttg aaattttcaa cctgcgtgcg gcaagacttt tattgccgcc
     7021 gcattcttca ataacttgct ttattaaatc ggatgtcgga cgtttttcat ttatttccgt
     7081 atgagttttt gaatatttta tctcttgctt tttatcgtga ataaacagtt cgtttgatgt
     7141 aattattgaa tttttgctaa gtaaaatcgc acgattaata acacttttta attcacgtac
     7201 atttcccggc cacgaataat tcattaacaa ttcagcagca tctgctgata acttaaaatc
     7261 ttgtgaattt agtttcaaaa aatgttcagc aagcaatata atatcatcac ctctatctct
     7321 taaagtcgga aggtgaagct ctacaggatt taatctgtaa taaagatctt ttcgaaaatt
     7381 ccctatttct acctcatgtc ttatatcctt attagtagcg catattatcc gtaaattaac
     7441 cggtatcgct tcatttgaac ctactcgggt aattgtacct tcctccaata ctcttaaaag
     7501 gtaaatctgc atatcaatag gcatttcaca gatttcgtca aggaacaggg tgcctccgct
     7561 tgatgcttca attttaccta ttcttccgcc ttttttagtg ccggtaaaag caccttcaac
     7621 atatccgaat aattctgaag atattaactc tttaggtatt gctccgcagt ttaccggcag
     7681 aaagattttt tcttttcggc agctgttatt atgaatagca cgagccaata cctctttgcc
     7741 tgttcctgtt tcacctgtta tcagtatggg catgtcagaa tctgaaaatt tatcagctaa
     7801 atcaattact tgtctaaatg ccggactttt accgattata catttaaact cgctgcgatc
     7861 actcgtcaat ttagcagagt tgccttgatt aagaaataaa ctttcttttt ggaaatatgc
     7921 tattgctcct ataatattat tacctatacg ccaaggaatg atagtaacaa tatatctttg
     7981 ctcagttttg cagcaaattg ttttataagt attatcaaac agttcatcaa atcgggagta
     8041 tttaggaaat acaaaactgt tttctattct atcccctact tttacaccta acatttgcgc
     8101 cgatttgttt attttactga ttttaccgtc aagcttgcag attatgcaag gctgctcatt
     8161 catttcaaac aaacaattca ttaaagacgt tgattcttct tgtaaatgat attcaattgc
     8221 attttctatt ttctgtgctt cgctgatagc ccagttaagc gtatgggcgg gaacaatatt
     8281 tttgtgtgtt gtaatagtta atgctcctaa tacgatttca gaagaaggtt cttttataat
     8341 agcagatgaa caatttattg aatgtgccat tttgcaataa tgctctgttg cataaatttg
     8401 tattggcttg cctgtatata aagatgtgcc tatactatta gtaccgcaat cgttttcact
     8461 ccacccggct cccaacaaat aattaatgcc gtgacaattc cctaacattg attttccggc
     8521 tctgatactc aaaataatac ccttttcatt gctgaaagaa actgccatat tttcatcgtg
     8581 aaatattttg aatatactat ccaggtatga atctataatc atgaataagt tatgctgtcg
     8641 ttctctttca accattatat catgttcatt taatttcaaa ggtgcacatt taagattgta
     8701 tggaactccc atttcctttg accgtttcca tgattctaag atgggatttc gaggcaagtt
     8761 aaaatcaggg ataatttcac cggcaatgaa attctcccat gctgattcga ttttatttaa
     8821 tgttgattca tagcttcgag ctgacaggaa aggaaaatca tcttctttaa gcataggttt
     8881 aacaccttat ttcacttaaa tttttattac ggattataat tgattataca attttttata
     8941 taccctgtca atacggatga aatggagcat tctttgcatc ataagcacgt tgagatttgg
     9001 cagtaacaag tgatgatatc ctttgactga tattttatgc taatttgtat ttttcgcata
     9061 ttttactgct ccactttgtg catctgtcac cgaaataccc gattatatca tcattttcat
     9121 aaatttttac cacttcacat ttattcgggc acccttcaca atcaaagctt cttgaaaata
     9181 tattgttatc ggcaagctta aggcctctga aatttgtttt gcctgtcttt tttactttat
     9241 tagcggcaat aattgcagct cctatcgctc ccatcatact gaagttttct ggaacatata
     9301 tttcaaatcc cagctcgttt tcaaaggctt ttttcatacc tttgtttgct gcaactcctc
     9361 cttgaaacaa aacctttgga agtattgttt tgcctttgcc tatattgttt aaatagtttc
     9421 tcaccagtgc atcacatagt cctctgatta tttctgcatg gttgtagcca agctgctgct
     9481 tatgtatcat gtctgattcg gcgaatacgg tacacctgcc tgcaattctt acaccggtac
     9541 ttgctttcat tgcataatcg ccaaattctt cgataggtat ttcaagcctt tccgcctgcc
     9601 tgtctaaaaa tgagccggta ccggcggcgc agacggtatt catggcaaag tcaacaacta
     9661 ttccgttatt aataattatt atttttgaat cctgtccgcc tatttcaatt atggttttta
     9721 catccttgtc aatttcaagg gctgctgttg catgagcggt aatttcattt tttacgacat
     9781 ctgcaccgat aattgctgaa gcaatcattc tgccgcttcc cgtagttccc actgcataaa
     9841 tttcagagtc ttcgtatttg ttttttagta ttttaaaccc ttcctgcact gctttaatag
     9901 ggtttccttt tgttcttaaa tatatttttt caatcaactc ataattatta tcaataagag
     9961 caatatccgt acttacagag cctacgtcaa ctcccatata atacattgtc gcgtctcctt
    10021 tccaataaat caatgaatgc ttcaactctc gtaatataac ctgcttctcc tgtcatatca
    10081 tcaactacca atgacattat cgggaagccg taatcctttg aaatagtagg aagaattgac
    10141 ttagatacta tttcaggcat acaacccata gggaaaattt gaattgcccc gtcaaaccct
    10201 tccttatatg caaggattgc ttcgcctatg cattccctgg catgacctcc tatttccatg
    10261 gataaatatt cccgggatga tttttttatt tttaaagaat ttaattttga aggttttaat
    10321 gccatgtctt ttaaccacca agaaggagtc attcctcttt tgctggatac accgtaatcc
    10381 attaatttat cttcgatata aaaatttgaa taaggctcta ttactgtgta tatttcacct
    10441 attagagcta tttttaaagg atccttgttt atatcgaccg gaatatattt cagtttattt
    10501 ctgtattctt ttattaatga aagcatttca taaggatttg ataatttaac cgcttctttt
    10561 aaacacttat gcaaaagtct tttgcattcg cctttatttc tttcatagcc tgccaaataa
    10621 tgagcaagcg attctgtttc atctattaaa ttcataattt caattgcatt ttttgcggct
    10681 tttaattttt caaacgagct ttttttactt gttgagccta ccttcacaag cctcttaagc
    10741 agttctttta atcctatatc cttgggcata tccaaaacaa tgaaattcaa gtcatatccc
    10801 aagtttttca ttaaattcat ctgaatctcg cagtattccc caaaacggca gggaccgcag
    10861 cttccagtaa gtattaccgt gtcggcaccg cgctcgattg cttgaatata attaccaatc
    10921 attattttaa aaggaaggca aatttcttca ggtgaatatt tagagcctat atttaaagct
    10981 tctgtactgt tttgaggagg aattacataa tcgatgtcca acccgtcaaa caaagcctta
    11041 actgcaatat aaatattacc catgtgtgga aatgttgctt tcattgaata atctcctttc
    11101 caacatatcc ctgaatgctt cgattcttgt attgattccc gcctcccctg tatgctcgtc
    11161 gatttttaat attaaaaatg gaaaatccgg tacaccgtct ttaataagtt caactattac
    11221 tgaatctgtt ccgcagttaa atgatgatat gtatattatt ccgtctacgt ttttttcttt
    11281 tgcggcattt acggcaaacc cgtagttttc tcttacaaag gtccaaaaag gtcttttata
    11341 taagttttcc gattcttttt gcaaaatgtc ttctccagta aactcagccg ttattatccc
    11401 cattcccaac gcgtttattt tttttaccaa attcatattt aaaaaactgt cataaatatt
    11461 gtagggatgt cccgtcaatg caatatttat ttcataaccg gaatcataga tgccgatttc
    11521 atgtgctttt tgtgcattta atgcatttat gaatgccctt tttatttcat aattattctt
    11581 tcttaagaat tttcccgatg atttagccca attataaagt tttttcttgg tggtggcata
    11641 aataggctct gttattgtta caggcagttt atcgaagcta ttgataacca tttccggcag
    11701 accgcaaaat ttgggacaga tgtattcgtt cttccacaat tgcataattc tcggaattac
    11761 cagtacatca catttatcct taattgaatc aacatgtccg tgaaatatct taaccggcaa
    11821 acatgcatca tccacacaat atttaactcc tgcatccaaa atgtttttgt ttgtatcatc
    11881 agagacaata atttcagctc ccaattcact gaaaaaagta actaaaaaag gatggtattt
    11941 gcaatacatc aatcctttcg gtattcctac cttcataaat cctcctttaa ataaaatatc
    12001 acaaattata ttatttccta taatttgtga tattatccct aaatttaaca tatttttgtt
    12061 atttcataat aattgagggt tattttgcag ctgttcctat tactaatttt atgcattctt
    12121 ctacgtcgaa tttattgaat atttctccgg aggtatgaag atttctggtg ggaatggata
    12181 ttacaccgct cggcactcca tctctggatg tatgaattgg tcctgcatcc gttccgccga
    12241 attccaaaat ttcatattga tatttaatac tatttttttc tgcaatatct gttaataagt
    12301 cttttacttc cgtatgtgta atgattgatt tatccattaa ctttattgct gctccattgc
    12361 ctaacttaac atccattttt attccgtcag gtgtatcgcc ggttgcagtt acatctactg
    12421 ctattgccaa atccggatta atgttgtatc ctgccgtttt agctcctctg cagcccactt
    12481 cttcctgcac cgaaaacaca taatatacat cattgtttgt ttccggatga ttttttagtg
    12541 cttcgattaa gacatagcaa cctattctgt catccgcagc cttgcatatt acacagtcct
    12601 ctgtttcgta atattctgat ttgaatacac acatatcacc tactcgaaaa ttctttgcaa
    12661 taaattcttt gtcggtagaa gcaacatcca aaaataattt gcttgttgaa agtttttcat
    12721 tatcttttaa ttcttcttga caaattactc cttcaagtcc attatcaaaa accatgcgga
    12781 gtcccaataa ttctttggca tttaatccgc ccacgtttgc aaaacgcaat aatccttttt
    12841 catcaacatg ggttataatc agtcctattt ggtccatgtg agcagaaaac agtatttttt
    12901 tgcctgtacc cttttttctt gctataaggt taccgagtgg gtctatggtt atttcatctg
    12961 caaaatcttt tatttcatcc ataatcattt cgcgaatcct tttttctctt cctgaaggac
    13021 cgaaacaatc ggataatctt ttcattaatt cgctgttaaa cattttattc ccctttctga
    13081 ttttcaataa tcacttgttt tgtaagcttt aaagcactca ttaagtcatt tttgcttgca
    13141 acgcttactg atgaatgtat atatctgcaa ggaactgaaa tggacactac ttttgcgcct
    13201 ttttttgttt tatggataac accggcatca tttgcccctg aagttgagcg tctgtactga
    13261 tatgcaatat tattgtcttc agcaattttt gcaactcttc ttacaaggtc gatatcatat
    13321 accgtagcat tgtccattaa tgacaatact gctcccttgc ctattaaggt ggagttttca
    13381 tgttcttcta cttcaggcat atctgcgcat acggttccct ccaatactat ggctaagtcc
    13441 ggctctaatt gatatgcggc tgtttctgct cctcttaatc cgacttcctc cataacggta
    13501 aatacgccat aaaaatctac atccaatttc tcttttagaa tctccaatat aatgctgcat
    13561 ccgaccctgt tgtccaaggc tttagcttta atcatattgt cgccgaattc aacataatcg
    13621 cttttaaaag ctgcgtagtc tcctatatta actaattttt ctgtttcctc ttttgtgctt
    13681 gtaccaatat cgatatatag tttatcaatt ggaagggaga cccctttttc tttttcagtc
    13741 atcaaatgta ctgctttaga gcctataacg cccggaatat tattttctcc tattaatact
    13801 acctttgagt ttaaaactct ttggtcaatt cctccaactg ctttaaactt aataaaaccg
    13861 tcgctatcaa tattagtaac tattaatcct acttcatcca tatgagcgga aaccataatg
    13921 gttttatttt ttttgccttt gttgtgagca ataatatttc caagcttatc tacatagaat
    13981 tgacagccca tttccgacag tttactcttt atataatttc ttacttcaaa ttcacagcct
    14041 gatacgccgg aaagtttagt taattcattt aaaaacataa tatttcctcc aaatcggaat
    14101 tgtccaattc atttataaac cttgctatag cttttcctgt aatttctaaa tcctttaaat
    14161 ctattgtttc cactgatgta tgcatgtatc tgagaggaat tgataaaagt atgcagggaa
    14221 ttccgtctct tgatatttgt agtgcatcag tgtctgtacc ggttctaaca ggaattgctt
    14281 catactgcat aggatagttg tatttcttgc aaatttcaac aaatttcttt gtaagttttg
    14341 tatttagcct tcctccgtat gcaacaattg ggcctttacc taagagagcc gaatcggaag
    14401 gcatatcggg agttttgcca aatcctacat ccaagactat tccgatatcg ggagcaattt
    14461 tataactgct tgttttggca cctcttaaac ctgtttcttc ctgagctgtt gcagtaaaat
    14521 atacattgct tttatgtgat atttttttta attcttttgc tgtttcgagc ataactgcaa
    14581 cacatgccct gtcatcaaat gctttggcgc ttataacgct tccctgtaat gataaaggtt
    14641 ctctgtttat tgttgcaata tctccaattg ttattaattt ttcaagtttt tcttttgtgt
    14701 accccgtatc tatatataat tcccttgttt taagcggttt ttttctttct tcctcacttg
    14761 ttaaatgagg cggtttaatt cctataactc caaatatttt ttcttttccg aatattgtta
    14821 catcctgggc aatcaatgat gcaggattaa atcctcccac cgtaatgaaa ctcaaaaatc
    14881 ctccatctaa tatgcctgta atcataagcc ctatttcgtc aatatgtgca gcaagcatta
    14941 ttttggtgtc cttactgccg gagcttttaa atatataact tccaagcctg tccttttcaa
    15001 aagaatcagt ataaggctta aaatatttta tcaaaatatc accgtttata tgctcaaaac
    15061 ctgaaacaaa ttggttggag catatatcaa acaaaaattc tttaagcatt ttgcctccta
    15121 tttttcaaaa aaaatgttag atcttgcgac ctaacattag aaattatttt aataaatcat
    15181 ccagtgcatc tcctatagat tctcctatag taaagtttgt atcttctgat tgataagatt
    15241 tattttctac agtttcttca cttttgtcat ctaatattaa tgtcaggtta aggtttttac
    15301 ctttcatatc aatactttgc actttagcat ctactatttc tccaaccttt acaacttgtg
    15361 ccggcgttct taatttttct gttgacacct ttgcaatcgg tatgaagcag tcaatatttc
    15421 cattagcgtt aacatgtata ccgtccaaga gtatacgtgt tacttcaacc tgtattatat
    15481 cgtccttatt tagtgtctta gcataaagat caaacggatg ctcagtcaat tgctttaatc
    15541 caaggccaac ctttctgtct tttctggtaa tgctaagtat ttttgcagtt acctcctgcc
    15601 cttcctggag aatatcagtt accttttctg ttcttgccca atccaaatct tttatgtgta
    15661 agagacattc aatatcatct actttaacaa atgctccaaa gtcaacaata cgagttacta
    15721 ctcctgttac aatatcgccc ttttgatatt tatccaagaa ttttgcccaa gggcttttta
    15781 cggttgcttt ataacttaat gaaagtcttt tattttcttt atctacattt tttaccttta
    15841 catttatttt gtcgcccggt tttaacactt cagacgggtg tttaattctg gtccatgcaa
    15901 tttcattgat gtgaagaagt ccatcgatac cgttgatttc tacaaatgca ccgtaatcct
    15961 gaaggttttt caccgtgcct tcgtaaattt ctccttcttg aatggtttcc catacttcat
    16021 tgtctttaat ttccttttca aatttttcaa cagctcttga agttaaaacc aactgtcttc
    16081 ttcctttttt attctttgtt tccaatatct taaccttcag atttttgcct acgtattccg
    16141 aagggtcaac atttcttttt attgaaattt gatttcttgg tataaaacct ttgatgcctt
    16201 ttagttttcc gtaaattccg tgctgagatg cttcaattat aggaatttcc acaatttctc
    16261 ctcggattct catctgctcc aaatcgtccc agattttctg ttcttccaat ggttttctgg
    16321 ttagtacaac atttccgtcc tggtcagaaa tttttactac aattgcatca atttcgtctc
    16381 cttcttttac tacttcagtt aaatcggaaa cttcttccaa ggaaaaatct tctcgtgtta
    16441 taattccgtc agatttataa ttgatgttaa cagaaacggc atcaggactt acatatatta
    16501 ctgttccttt aaccttttcg ccgattccga gattaactac tccgccataa ttttcaagca
    16561 attcatgcat actgtttact ttttctgctt tatcagtctg ctcaacagtt tctgtctttt
    16621 ctgtttcctc ggattgctct actaattctg tctgttcctt tttatcagac agttctgccg
    16681 gctcagactg ttcagctaaa tctgcatgtt ctgcctgctc gttcatttca ttcatgttgt
    16741 tattcaaatt ttccaacata ttaataacct cctcaattat ccagtcagga gctgaagcac
    16801 ctgcggatac tcccacaata gaatttttat caatatagtt ataaggtatt tccttataat
    16861 tttgtattaa gaatgtgttt tcgcacaatt ccttgctcag ttcatataac tttttggtgt
    16921 ttgaactgtt tttaccgcct acaataagca ttatatcaca tttggaagca agagcaatgc
    16981 atgcttcttg tcttttcttt gtcgcatcgc atattgtatt atgtacaatt atattagaat
    17041 aattgtttat ttcaatagtt tttaatattt cttcaaaagt atttaaatta aatgttgtct
    17101 ggcagactaa acagtatttt tttgtcggct caattatatt ttttagttct tcagggcttt
    17161 taataattac ggcttcatag ttgcaccagc ccgatattcc ctgtacctca ggatgatttt
    17221 catcgccgac aatgattata ttgtaatctt tatttttgaa ttcttcaact attttatgta
    17281 tttttttcac ttttacacaa gttgcatcaa taacttcgat gtttttttct tccaatctgt
    17341 catatatatt ctttcctaca ccgtgacttc tgataagaaa ttttgcatcc ttgttttcag
    17401 gaatatcgtc aacaataacc attcctttgt tttttaattt ttcaacaaca tctttgttgt
    17461 gaattatttc tccgtaacag tatagttttt tttcgtcagt caaaatctta tccgctttca
    17521 gtacggcagc cttgactcct ccgcaaaaac cgttgtattc tgaaacaatt attttcattt
    17581 cataccttca ttttttaatc tgtaaatatc ctttaaaatt tctattccta tttccgtata
    17641 atcttcagaa ttcagcctgc cgtcgtaatt tttaaagtag tctttttctt ctcctatata
    17701 cagtctgagt tttcctctga atttataggg tccatccaaa taaacgggca taattccaac
    17761 ttttgcttta ttagctatta aggcaattcc cgattttgca tttttttcgt caaatccggt
    17821 tttaattctt gttccttccg gatatattcc taatataccg cccttattta aaacattaag
    17881 ggagtttttt attgccgata aggatactcc tttcctgtcg acgggaaagg caccaagact
    17941 tctgaataaa aatgatagga atttgttttc aaacaattct tttttgccca tgaagtgaat
    18001 ttgccgctta gtagatactg ctaatattac agggtcaatc atggatctgt gatttccgca
    18061 aattattaag ccaccgtcta tattgtcaag gttctctctg ttatatactt ctaagtcaaa
    18121 aattataaat acaagaacct ttaatacaat cactgctatt ttataaaaca ttgcttcctc
    18181 cgatatattg tgcaatagtc tttgtaactt cattcaagtc catattagtt gtgtctatta
    18241 atattgcatc ttcagcaggt ttaaggggag aaaactttct tgttgaatca ttttcgtcac
    18301 gcttaatcat atcattgata acatttgtgt aatcagcttt aatattgttt aatttcaatt
    18361 gctcgtaacg cctttttgct ctctcttcca atgatgcagt tatgaaaaat ttgtgctctg
    18421 catccggaaa aacaattgta ccaatatcac gaccttccaa taccacattt ttattttttg
    18481 caatctttct ttggatttca accatttttt ctctgacaat ttttaaagat gatatttttg
    18541 aagccgctgt tgatatttct tcatttctta ttaagttttc aacattttca ccgtctaaaa
    18601 atactttatt gtcattgtag gtaatattgg ttgtttcaag caattctgca atattagctt
    18661 catttatgtc aatattttgt ttatatgctt tatatgcaag agctcggtac atagcccctg
    18721 tatcgatgta ctcatatcca agttctttgc ttaatagtct tgcaacagtg ctttttccgg
    18781 ctcctgacgg tccgtctatt gcgattatca tattgttttc ctttcataat atataagtat
    18841 ggtaaaacca aacttagtat ttactgttgc acataatgtc cctattatgt attcaacacc
    18901 ttaacattat agtacatatt attaaatata acaagaaaat tttttggcac agttaaacaa
    18961 aaggatacaa agtatagggt atttccttaa cgctcaataa acatgctctt gttataagcg
    19021 tctccccttt ctttatatcc ttaatgatta ttcttacttt agagcg
//
LOCUS       DAOSHP010000030        19051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2334_length_19051_cov_7.870710, whole genome shotgun sequence.
ACCESSION   DAOSHP010000030 DAOSHP010000000
VERSION     DAOSHP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19051
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_19051_cov_7.870710"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..555)
                     /locus_tag="PLB33_04575"
     CDS             complement(37..555)
                     /locus_tag="PLB33_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOG62831.1"
                     /translation="MTSEERRARIEEILKNSTEPVTASYFAKKFSVSRQVIVGDIALM
                     RAGGLKITATPRGYVFDKEEESKCVFTVACCHDEKNLGDELFAIVDNGGTVQDVIVEH
                     PIYGEIIGELHIRSRYDVELFLKKIAEKKVQPLLRLTDGIHLHTIKCKDIETKNRIIN
                     VLKLKNIMLEQK"
     gene            complement(638..711)
                     /locus_tag="PLB33_04580"
     tRNA            complement(638..711)
                     /locus_tag="PLB33_04580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Pro,seq:cgg)
     gene            905..1384
                     /locus_tag="PLB33_04585"
     CDS             905..1384
                     /locus_tag="PLB33_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62832.1"
                     /translation="MYDRYMPYLANNKMLDLTYKKLKSLESPQIGYLKLNTYRHDTRS
                     PISNAFVRVSKLTISGYYREIGTGVFLAANSSDENGSVPIFELPVLTNENEMYNVSVN
                     YLDYCPAYIIDIPIYPGVTTTYDVYMHHYSMNGDTDMDYHFILQPQIPGRNTGPEIF"
     gene            complement(1402..2856)
                     /locus_tag="PLB33_04590"
     CDS             complement(1402..2856)
                     /locus_tag="PLB33_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018671001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOG62833.1"
                     /translation="MTNTRIKTSWNLENSYAELPEFFYSKINLSKVSSPKLVVLNNEL
                     AEYLGLDINELQSEEGVSYFAGNSVPKGSIPIAQAYAGHQFGYFTMLGDGRAMLLGEQ
                     ITPNGKYDIQLKGSGRTPYSRGGDGRAALGPMLREYIVSEAMHALDIPTSRSLAVVTT
                     GEPVIREKILKGAILTRVASSHIRVGTFEYAANFGTEENVRQLANYTIDRHYKEIEKD
                     DFPYLSFLKEVVKRQASLIAKWQLVGFIHGVMNTDNMALSGETIDYGPCAFMDAYNPN
                     TVFSSIDTYGRYVYKNQPKIAVWNLSRFAETLLPLLHEKNDKAIKLAQDAVAEFYELY
                     HNNWISGMRKKLGIFNEEAEDEALIEELLNIMHKNNEDYTNTFIKLTFGNTLNDSAEY
                     KEWHNKWQARLQRQNKTKDKLFELMKNSNPAVIPRNHRVEEALRTADENEDYSVMDKL
                     ISVLKNPFAHSADQKEYAELPPPSFCNYKTFCGT"
     gene            complement(3001..3870)
                     /locus_tag="PLB33_04595"
     CDS             complement(3001..3870)
                     /locus_tag="PLB33_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006438970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOG62834.1"
                     /translation="MNIIQKDNKITIHDIRDFNITHIFECGQCFRWNREDDGSYTGVV
                     KNKVINVLQQENTVEFNNINTDDYDIIKNYFDFDTDYETIKNTLNTDEIMAEAIKFGE
                     GIRILNQEEWETMVSFMISANNRIPMIKKVIENLSVSFGDYIGNYRGKDYFSFPTAER
                     LSAAPVERILECKAGFRAPRIKEAATRFLYEKDKIYNIKNMSYNEGLAYLKTYKGIGD
                     KVANCILLFSMKHFDTFPVDVWVRRVMQTLYVSKETKDADIRKFAENKFGKYSGFAQQ
                     YLFFYARENGIGK"
     gene            complement(4096..4728)
                     /locus_tag="PLB33_04600"
     CDS             complement(4096..4728)
                     /locus_tag="PLB33_04600"
                     /inference="COORDINATES: protein motif:HMM:NF037888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62835.1"
                     /translation="MSTNQDFEKINQELLNIAFNCYWIESYLVINDKIDSCMKFEHLS
                     DCKELFITIEKALTEASIIALARIYDINSKGTNLDKILKNACNEIPMDEDKYNEIKIY
                     CRDKRRELNNSEYSELLYNLRKWRIKYYAHTDIEFLNNEINLRNSYKINLEEFKKLIN
                     FAKRTSEHLYFLINDVSIINSITIKCEKGSDLLIKILEEHKQLYDNAYNE"
     gene            complement(4869..5381)
                     /locus_tag="PLB33_04605"
     CDS             complement(4869..5381)
                     /locus_tag="PLB33_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62836.1"
                     /translation="MDLNINDFLDLHKDLRGDFDAVYFGINNIKKKLQRYSDGKNLKG
                     DEIVGWYGEICCRMLTGGKLVSDNLEYDVLTSSGDRISVKTRKGNGSGWNTTSAIPKI
                     DMDDESPTHLMFIHLADNYSVKEIWLFSWEELHRNNRFIEHKVRGNRRSYLVRINKAT
                     DDKYLIYKAE"
     gene            complement(6355..6591)
                     /locus_tag="PLB33_04610"
     CDS             complement(6355..6591)
                     /locus_tag="PLB33_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62837.1"
                     /translation="MKWDYKMIVSEPLYSSPMKGPNITKLIEGSKFFAGKHEPIWNQI
                     LEIGDEGWELINVVPTIWDGNTYNIAFFFKRPIE"
     gene            complement(7454..8125)
                     /locus_tag="PLB33_04615"
     CDS             complement(7454..8125)
                     /locus_tag="PLB33_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOG62838.1"
                     /translation="MNDKQMQSNYIKIVGSIFSNLEYSHEVFGEKFYEFFIEVPRLSE
                     TKDILPVIISERIINDINMDIGNHIVIEGQFRSYNRYEDDSNRLLLRIFVRDIYVPDE
                     TELEEMKRHPNEVFLNGYLCKETKFRTTPFGREITDMLIAVNRSYNKSDYIPCIAWGR
                     NARYCEKLEVGDHIKLWGRIQSRKYQKKNEHDIYETKTAYEVSITKLEHIKEENNRKK
                     DFEES"
     gene            8383..9474
                     /gene="ychF"
                     /locus_tag="PLB33_04620"
     CDS             8383..9474
                     /gene="ychF"
                     /locus_tag="PLB33_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOG62839.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITSAGADSANYPFCTIEPNIGVVEV
                     PDKRLNFLTKMSNSGKTVPAVIEFFDIAGLVKGASKGEGLGNKFLSHIREVEAVVHVV
                     RCFEDENVIHVETTVDPKRDIEIIELELVLADLEVMSKRLDKLSKMVKNDKSLQAEFE
                     LVQKIVQGLENEKQARYLSYTDEEQKLLKTFNLITSKPVLYVANVDESYLSDPSGNKY
                     VDIVKEYASRDNSEVIVICGKIEEEISVLDEDEKKEFLEEMGLKESGLDKLIRASYRL
                     LGLISFFTTGPMESKAWTIVKGTKAPQAAGKIHSDMERGFIRAEVTKYDDVVKLGSMH
                     AAKEKGLMRLEGKDYVMEDGDIVLFRFNV"
     gene            complement(9569..10246)
                     /locus_tag="PLB33_04625"
     CDS             complement(9569..10246)
                     /locus_tag="PLB33_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOG62840.1"
                     /translation="MAILERFGDIIKANINVLLEKMEDPEKMIDQYLEDMMKDLAEVK
                     QSTAEVIAEETRTKRLVEQNEAEVAKYNEFAKKALVAGNEDDARTFINKKQELENAGA
                     GLMTAYAAAHENAIKMRQMHDKLTADIETLRARRQVIKSKVKVAKTQETLNEATQATK
                     KIAGAKSAFERMEDKANRMLDEANAMAELNMEPIDEAKALEEKYAKMGNASVDDEISK
                     LKEELGL"
     gene            complement(10285..11346)
                     /locus_tag="PLB33_04630"
     CDS             complement(10285..11346)
                     /locus_tag="PLB33_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOG62841.1"
                     /translation="MARGGGSFGGGGRGGGSFGGSRGGGSFGGSRGGGSFGGRSGGSF
                     GGRSGGSFRGSSSGGIFGSSKSTGTGFGPVFGPRIPMGGGFGGGRPQRSSGGGGCGCV
                     TLIVVLIVIAVIFIVIFSLWGSMNSSGGGDITFSSVKRDPLPKGSVNETEYYTDRLGW
                     INNETKLISGLKHFYEKTGVQPYLYLTDTINGSHFPTEQDLEAFATDLYDELFTDEAH
                     LLIVFFEYEGQYKGWYIVGTQAKSVIDAEAGNILADYIDRYYYDSSLSNEEFFSKAFT
                     DAADRIMTVTRSPWITVFIVLGVVVLVIVLFIWWKKHKEQKNLEAKRREEMLNTPLER
                     FGTTEAEDLMKKYQDDNEQ"
     gene            complement(11381..12205)
                     /locus_tag="PLB33_04635"
     CDS             complement(11381..12205)
                     /locus_tag="PLB33_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62842.1"
                     /translation="MDKYEEIERSITKRFRKLIWAKFISAIKEYKLIQDNDKIAVCIS
                     GGKDSMLMAKCMQQLQKYSEIQFETEYLVMDPGYNDANKKRIIENAEILNIPIKIFNT
                     PIFEIVDRVEETPCYLCARMRRGYLYRNARELGCNKIALGHHFDDVIETVLMSLFYAA
                     EIKTMMPKLHSTNHEGMELIRPMYKVREDDIIAWKRYNNLQFIQCACRITERIKAEGT
                     GSKRQEMKILINKLRQINPNIDTNIFKSIHNVNLDTIIGYRKGNVYRNFLDDYDKN"
     gene            12367..13170
                     /locus_tag="PLB33_04640"
     CDS             12367..13170
                     /locus_tag="PLB33_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOG62843.1"
                     /translation="MGIAHEVDYKIYGDDIQFVEIGLDYGETIVAEPGAMMYMDSSIE
                     MDTMFGDGSEKSKGFKGMLATAGKRVLTGENLFTATFTNTSIDKKVVAFAAPYPGKII
                     PVNLPDYGGRLICQRDAYLCSAKGIEITIAFQKKIGAGLFGGEGFIMQKLVGDGFAFL
                     HAGGTIVKKELASGEMLKLDTGCLVAFTEGVDYDVQFASNVKSALFGGEGLFLATLRG
                     PGTVWMQSLPFSRIADRIYSASRKSGGGGSKGEASLLGNIGIGNLFGGD"
     gene            complement(13228..14133)
                     /locus_tag="PLB33_04645"
     CDS             complement(13228..14133)
                     /locus_tag="PLB33_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOG62844.1"
                     /translation="MKYLIGIDSGGTKSELTAYDLNNAQVFNLIGEFGNPAVNLDKTV
                     NNLISLIYDCVNELGREECQLIAVGMAGVETGNYAELIKKYVEGAFVIKTIVLNDAEM
                     ACKAYFGDKDGILAISGTGSSCFAQKDGAGEMVGGWGHILGDEGSGYHTVINVIKNIV
                     YKYDRALPFDDLSKAVMEEIGGSTRSDIMNFIYNNEKNKIASLFPIISDYAEKGDAYA
                     TMLLENAGKYLAELTSVAYKKKNFKGTANIGLKGGVFHNSSIMLSAYKDEIKKRLDSY
                     EIIEEDISTTKAVCNIFKNEKEGAR"
     gene            complement(14292..14480)
                     /locus_tag="PLB33_04650"
     CDS             complement(14292..14480)
                     /locus_tag="PLB33_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOG62845.1"
                     /translation="MRANVDRDACISCGLCESICPDVFELDDEGISVVKVDPVPSEFE
                     DCAKEAEEECPTDAIHVE"
     gene            complement(14688..15887)
                     /gene="megL"
                     /locus_tag="PLB33_04655"
     CDS             complement(14688..15887)
                     /gene="megL"
                     /locus_tag="PLB33_04655"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009087 - methionine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009093 - cysteine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HOG62846.1"
                     /translation="MDKDKLSRMHFSTRAVHAGYRPNEYGALATPIYQTSTFIFDSAE
                     QGGRRFALEEEGYIYTRLGNPTCSQVEDKLASLENAEAAVAMASGMGAITSVIWSIVS
                     AGDHIVAAKTLYGCTFAFMSHGLTKFGVEVDFVDTSDPENVRKAMKPNTKIVYLESPA
                     NPNMLLSDISVISKIAHETEGCMVVVDNTYCTPYIQRPIDLGADVVVHSATKYLNGHG
                     DVIAGFAAGKKEFIDKVRLFGIKDMTGSVMSPFDAYLINRGMKTLEVRMEKHCSNAQK
                     VAEFLESHPAIEWIGYPGLKSFPQYELAKKQMSLPGAMIAFELKGGLESGRKLMNTVE
                     LCSLAVSLGDTETLIQHPASMTHSPYTPEERALSGISEGLVRLSVGLEHADDIIDDLK
                     QALDKLL"
     gene            16236..17561
                     /locus_tag="PLB33_04660"
     CDS             16236..17561
                     /locus_tag="PLB33_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOG62847.1"
                     /translation="MDFIIAVIIFIFSLIYNISKQYSLIIPLLIGMLAFSSVAFYRGF
                     KLRNIVVMLMKGMKKSLYILSIFALIGMITALWRAGGTIPFFVYYGIKIMNPDYFILF
                     AFLLTCFVAFALGTCIGTAGTVGVVLIILARSGGVNIYVAAGAILSGAFFGDRSSPLS
                     SSANLVAIITETDLYDNIKKMFITGALPFLATIVVYLLLSKNNPINVTNNELIEKFSV
                     NFNLHISTVLPAVIILLLAISKNDVRVAMLFSIITAVFICLYIQNLTLQEILKYMIFG
                     FVNTDPALSEIISGGGLISMLNVTFVVLIASSYSGIFEGTGMLKDIQGALIKMSNKIG
                     VYLTTVFTSLVTCACCCNQTLSVLLTHDLMNNIYKENNLTKDNLAIDIENTAILIAAI
                     FPWSVAVAVPLATMDVGAKAIFYAVYLFFVPLMAPLIPKSKVYNLGDGN"
     gene            complement(17595..18863)
                     /locus_tag="PLB33_04665"
     CDS             complement(17595..18863)
                     /locus_tag="PLB33_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG62848.1"
                     /translation="MYIHVVQKGDVLWKIADRYNVSIQDIVDLNKLSNPNILLPGHAL
                     LIPTKNTYTVRRGDTIWKIAQKFGVNIQDLLAENRNINQDLIYPGNVLTIPQRPKPEI
                     DVNAFTYFLGSEAVPIVRGAGPYLTYLSPFAYLINEDGTLVNIADEPAIAEAYSQGVV
                     PMMAIVNFTVNVEGENLANIVLNNPDTIDTLQENIINIMREKGYQGLNIDFEYVLPED
                     REAYNSFLESTVNRLHNEGFFVSTSLAPKISGEQVGLLYEAHDYPAHGRLADFVVLMT
                     YEWGWRGGEPRAISPINEIRRVLDYAVTVIPRDKILMGFQIYARDWRIPFQEGDEAET
                     ISVQDAYNLAYEHMSEIHYDYVSQSPYFYYTDAEGNSHVVWFEDARSAQAKFDTVKQY
                     GLRGISYWGLGYEFPQNFPLLADNFQIKKL"
BASE COUNT         5738 a         3627 c         2866 g         6820 t
ORIGIN      
        1 taagcataat attatcaaga taacttctta tgattgctat ttctgctcta acataatatt
       61 ttttaatttc aatacattta ttattctgtt ttttgtttct atatctttgc atttaatagt
      121 gtggaggtgt ataccgtctg taagtctcaa gagaggctga actttctttt ctgcaatttt
      181 ttttaagaat aattctacgt catatcttga cctaatgtgc agttctccga taatttctcc
      241 atagataggg tgctctacaa taacatcttg cactgttcct ccgttatcaa caatagcaaa
      301 cagttcatcc cccagattct tttcatcatg gcagcatgcg actgtaaata cgcatttgct
      361 ttcttcttct ttgtcaaaaa cataacctct tggtgttgca gttattttga gaccgcctgc
      421 ccgcatgaga gcaatatctc ctactataac ctgacggctt acggaaaact ttttggcaaa
      481 atatgaagca gttacaggct ctgtgctatt ttttaatatt tcttcaattc gtgctcttct
      541 ttcctctgaa gtcatagtaa gcctccttta cgcagggatt accaatttag tcatatacaa
      601 atgccggcaa atattttgcc ggcaccttgt tgaaaaatcg gagtgaaagg atttgaacct
      661 tcggcctcat ggtcccgaac catgcgcgat accaagcttc gctacacccc gacaacacta
      721 acattataag ctctatgaaa atttttgtca atggaatttt cttgttgtcc acattcggtt
      781 cgttaaatct gcacatgaga ttaaccatca atatttaaaa cacatttaaa cttttgacat
      841 attataataa taaattatat gtacttttta ttttgtatta ttctaaattt tttggaggtt
      901 accaatgtat gacagatata tgccatattt ggcaaataat aaaatgcttg accttactta
      961 caagaaacta aaaagcctcg aatccccgca aatagggtat ttaaagctca atacttatcg
     1021 ccatgacaca agaagtccta tttccaatgc atttgtcaga gtatcaaaac ttaccataag
     1081 cggttattat agagaaatag gaacaggcgt ttttttggct gcaaattctt cagatgaaaa
     1141 tggttcggtt ccaatttttg aacttcccgt attaacgaat gaaaatgaaa tgtataatgt
     1201 atctgttaat tatctggatt actgccctgc ttatataatt gatattccga tatatccggg
     1261 tgtaacaacc acatatgatg tgtatatgca ccattattct atgaacggtg atactgatat
     1321 ggactatcat tttattttgc agccgcaaat accgggccga aatacagggc ccgaaatatt
     1381 ttaaatctgg tgtcattctt tctatgtgcc gcaaaatgtc ttataattgc aaaatgacgg
     1441 cggcggaagc tctgcatatt ctttttggtc agctgaatga gcaaaaggat tttttaacac
     1501 gcttattaat ttatccataa cactgtaatc ctcattctcg tcagctgtcc ttagagcttc
     1561 ttctaccctg tgattgcggg gtatgacagc agggttggaa tttttcatta attcaaataa
     1621 tttatctttt gttttatttt gcctttggag gcgtgcctgc cacttattat gccattcttt
     1681 atattctgca ctatcattca gtgtattgcc aaaagttaac ttaataaaag tatttgtata
     1741 gtcttcatta tttttatgca ttatattaag aagttcctca ataagagctt catcttctgc
     1801 ttcttcattg aaaattccca atttttttct catgcctgaa atccaattgt tatgatataa
     1861 ttcataaaac tcggcaactg catcctgggc aagcttaatt gccttatcat ttttctcatg
     1921 caaaagcggc agaagagttt ctgcaaatct agataaattc caaacagcta ttttcggctg
     1981 atttttgtaa acatatcgac cataggtatc aatagaagaa aaaacagtat tggggttata
     2041 agcatccatg aaggcgcagg gaccataatc gattgtttca ccgcttaatg ccatattatc
     2101 cgtgttcata acaccgtgta taaatccaac cagctgccat tttgcaatta atgatgcctg
     2161 cctttttaca acctctttca aaaaactaag atatggaaaa tcatcctttt caatttcctt
     2221 atagtgtcta tcaatggtat aattagccag ttgccttaca ttttcttcgg ttccgaaatt
     2281 tgctgcatat tcaaatgtac cgacacgaat atgactgctt gctacgcggg ttaaaattgc
     2341 gcctttaagt attttttctc ttattacagg ctcaccggtt gtcacaaccg ctaaacttct
     2401 gcttgtagga atatccagag catgcattgc ttcgcttaca atgtattcac gaagcatggg
     2461 accaagcgca gctcttccgt ctcctccccg cgaatatgga gtcctgccgg agccttttaa
     2521 ctgaatatca tatttcccat tcggtgtaat ttgttctccc aagagcatgg ctcttccgtc
     2581 tccaagcata gtaaagtaac cgaattggtg tcctgcatag gcttgcgcaa ttggtatgga
     2641 gccttttggc acagagtttc ccgcaaaata agatactcct tcctcactct gaagctcatt
     2701 tatatccaaa cctaaatatt cagctaattc attatttaaa acaactaatt ttggtgagct
     2761 gactttgctt aaatttattt tggaataaaa aaattccggc agctcggcat aactgttttc
     2821 taaattccag cttgttttta tccttgtatt tgtcattttg tctcctatat tatcttttta
     2881 tctattatat acgtgaagac ttctgtttaa cctgtatttt tatttgcaaa tttacaaaat
     2941 ataggttatt ttaataattc aaaaaatgca gcaaaagctg cattaaatac tgaagacata
     3001 ctacttgcca atcccgtttt cgcgagcgta gaaaaaaaga tattgctgag caaatcctga
     3061 atatttgccg aatttatttt ctgcaaactt tcttatatct gcatccttag tttctttact
     3121 tacatatagt gtctgcatta ctcttcttac ccaaacatca acaggaaatg tgtcaaaatg
     3181 tttcatcgaa aataataata tgcagttagc caccttgtcc cctattcctt tataagtttt
     3241 aagataagcc agtccttcat tatagctcat atttttaata ttataaattt tatctttttc
     3301 atacaaaaac cttgttgctg cttccttaat tctcggagct ctgaagcctg ccttacattc
     3361 aagaattctt tcaacaggtg cagccgataa tctttctgca gtcgggaaac tgaaatagtc
     3421 cttccctcta taattaccta tataatcccc aaagctgaca cttaaatttt caattacttt
     3481 ttttatcatt ggaattctgt tgtttgctga aatcataaag gaaaccattg tttcccattc
     3541 ttcttggttt aaaattctta taccttcacc gaattttata gcttccgcca ttatttcatc
     3601 cgtattcaat gtattcttaa tcgtttcgta atcggtatca aaatcaaaat aattttttat
     3661 aatgtcataa tcatcagtat ttatattatt aaactcaaca gtattttctt gctgcaaaac
     3721 atttatcact ttattcttaa caaccccggt ataagagccg tcatcttctc tattccatct
     3781 aaagcattgt ccgcattcaa aaatatgagt tatattaaaa tctcttatat catgtatagt
     3841 aattttgttg tctttttgta taatattcat cattaacacc ttaatttctg tcttttatca
     3901 ttatagcttt gggcctgtcc cccaatttcc atcaagcata tagaatcata actttcatgg
     3961 aaactttaat attaattgta tcataatatg tcagattaat aaattgtttt attataaaaa
     4021 gaaaccttgc ggtttctaat atatctttta tgctattaat ccaaagaaaa atttttttat
     4081 ttcaataaag attgcttatt cattataagc attatcatat agctgtttat gttcttcaag
     4141 tatttttatt aataagtcag atcccttttc acattttatt gttatcgaat tgattatact
     4201 tacatcatta attaaaaaat ataaatgttc agacgtgcgt ttagcaaaat ttattaattt
     4261 tttaaactct tctaggttaa ttttatagga atttcgcaag tttatttcat tatttaaaaa
     4321 ctcaatatca gtatgtgcat aatactttat acgccacttt cttaaattat ataagagttc
     4381 gctatattct gaattattta attctcttct tttgtcccta cagtaaattt taatctcatt
     4441 atacttgtcc tcatccatag gtatttcgtt gcatgcattt ttaagtattt tatcaagatt
     4501 tgttcctttg ctatttatat catatatcct tgccaatgct attattgaag cttcagtaag
     4561 tgctttttct atcgtgataa atagctcctt gcagtcactc agatgctcga atttcataca
     4621 actatctatt ttatcattta taactaagta agattctatc cagtaacaat taaacgcaat
     4681 attaagtaac tcttgattaa ttttttcaaa atcttgattt gtactcatac aattaattcc
     4741 tcataacaaa ttatttatct ttgtctccag tatctactaa ttcatcaggt cgttaatttt
     4801 aatatttttt ttacatatac agcttccagt aaatttatcc tattaatatt ttttttgata
     4861 atgtcagttt attctgcttt ataaattaga tacttatcat cagtagcttt atttattctt
     4921 acaagatatg accttcgatt acctcttacc ttgtgctcta taaacctgtt attgcggtgg
     4981 agttcttccc agctgaatag ccatatctct ttaacactat aattatcagc taagtgaata
     5041 aacattaaat gggtgggtga ttcatcatcc atatcaatct taggaattgc acttgttgta
     5101 ttccagccgc tcccatttcc ttttcttgtt ttcacagaga tcctatctcc tgaagaagta
     5161 agtacgtcat attcaagatt atctgaaact agtttacctc ctgtaagcat acgacaacat
     5221 atttcaccgt accatcctac aatttcatct ccttttaaat tctttccatc tgagtacctt
     5281 tgaagctttt ttttaatatt attaatccca aaataaaccg catcaaaatc ccctcttaaa
     5341 tctttatgta agtctaaaaa atcattaata tttaaatcca taatttttac cccccataat
     5401 ataaatttta tacttacaca tagattttcc aatgatttta aaacatatta gcctaattat
     5461 tctatacgtt tttacttata tttctaatta ctccagatac ttctataaaa acaattatat
     5521 catgatgtat gctattttta caacgaaaat aaactataaa ctttattatc ctaatctgaa
     5581 caggtgcaga gtatagaaaa tatatatttt atttagtttt gaacatatta tcgaaatatt
     5641 gagtacaatt aatcacatat aaaactaact aaaaagaaac cttgcggttt cattaaactt
     5701 aaattataca ttgaaatttc aaggttattt tttatatgtt tgcatgtctt tttttatatt
     5761 ctaaccttaa aaaggcaact atgtagaata attttcttta tcttcaatgt ctactgtttt
     5821 aatatggtcg tctaacattt cagtatatat atgctgaagc tgtggatatt ttacaaaatt
     5881 aaatagaaac cttttcaaga ataattatat ttaacatgga caagggcatt tgctttatgg
     5941 tatatacatg ggatggttga tatctatacc tttaaacttt accacaccta aaaaggttct
     6001 ttattttata tttatgacta taggattgct tttacctgat attgacttgc ctaatacaac
     6061 atatggaaaa cggataaaac taatttctaa atggattcat agcatggtcg ggcatagaac
     6121 attaacacat agtctatttt tcataacagt tttgtattat tcttgtaata ttgcttttgg
     6181 aataaacata tatactacag gattaatgat aggaagtgtg attcacattt tctgtgattt
     6241 atttactcca agtggagtcc ctttagcata tcctatcagt gataagaaat ataaattata
     6301 aataatttta taataaagag gctatctcaa tagagacagc ctttcctcaa attcttattc
     6361 tataggtctc ttaaaaaaaa atgcaatatt gtacgtatta ccatcccata tagtaggaac
     6421 aacattaata agttcccatc cttcatctcc aatttcaaga atctgattcc atattggttc
     6481 gtgttttcca gcaaaaaact tcgagccttc tattagtttt gttatgttag gtcctttcat
     6541 cggtgaagaa tacaatggtt cagatactat cattttataa tcccacttca ttttcactct
     6601 acctcgtttt aatttattta atcaatggta aaattgaatt ttcataatta tatattatca
     6661 gcaaaaactg gagttgttac cattagtaag caaccaacta ataaaccaat tagaaaaggt
     6721 ctgagattct ttttcattag acttcctcca ttttttattt tagtataacc caattggtaa
     6781 aattatgcaa ttggctatat taaataatct tctaatttat tcaattattt gtaatccgta
     6841 acctttttct gaataaataa aatcatttca aagttttttt attgatttac tttcaaatgt
     6901 atttagcttg ttcgtatgaa atttttaata gtatggcaac attcttctat tgttcacttt
     6961 ttataaaacc tttcattgat taaattatat tcctaattga caatccttct aaagccttat
     7021 tgacaatctt atttaggtct tgctttttta attaatataa aattttcatt ttctgttgct
     7081 gtacttacag agtataaatc taatatagta atatcctctt attcatttaa gggtccatct
     7141 aaatgatatt gtgttttgca tagcaatttt aattttttct aactttatac tgctaatatt
     7201 caccgtatta gcattttcct aatttattgg ctctcttgac aagcaatagt gaaaggtatt
     7261 taaaaacact ttaacaacat tgacaacatt tctgacaacg ctatgctgat aaattatgat
     7321 aattatgaga ttataaaaat ataataatac attgaatttt cataatatat agaaacttgg
     7381 gggtacattt caatgtctat ataatttact aaaaaagaaa cctttcggtt tcttgtatta
     7441 ctctatatta taattacgat tcttcaaagt cttttttcct gttattttct tcctttatat
     7501 gttcaagctt tgttatcgaa acttcatagg ctgtctttgt ttcatagata tcatgctcat
     7561 ttttcttctg gtatttcctg ctttgtattc ttccccaaag tttaatatga tctccaacct
     7621 ccaacttttc acaatacctt gcattcctcc cccatgcaat gcaaggtatg taatcggact
     7681 tgttatatga acgattgact gcaatcagca tatcagtaat ttctctgccg aatggagtag
     7741 tcctgaattt agtttctttg caaagatatc cgtttaaaaa aacttcgttt ggatgccttt
     7801 tcatttcttc aagttcagtt tcatcaggta catatatatc tcttacaaat attcttaaca
     7861 aaagcctgtt tgaatcatcc tcatatcggt tgtatgacct gaactgacct tcaattacta
     7921 tgtgatttcc aatgtccata tttatatcat taattatcct ttcggatata ataacaggca
     7981 gtatatcctt ggtttcactt aatcttggaa cttctataaa aaattcgtaa aatttttcac
     8041 caaatacttc atgactgtac tccaaattgc tgaatatgct cccgactatt tttatataat
     8101 tggactgcat ctgtttatca ttcatataat tttttccccc tataatttgt cttatatatt
     8161 ttattaatat tcattgcaat aaaatatatg ctttaaaagc caaaaagttg tctatttttt
     8221 agtattccta atgcaaattt atttaatgtt gaactttaaa atttaatatt atataatgaa
     8281 tattattaaa aatcagtttg cgtaacagga taacaaataa gaacatattc atttattgta
     8341 agtactaacg tgtaaatttg aagaaaggat ggtcacacca tcatgaaatt aggtatagta
     8401 ggacttccta atgtaggcaa aagcaccttg tttaatgcaa ttacttcagc aggtgcagat
     8461 tcggctaatt atcccttttg cacgatagag cctaatatcg gagtagttga agttcccgat
     8521 aaaagattaa attttttaac taaaatgagc aattcaggga agacagttcc tgcagttata
     8581 gaattctttg atattgcagg cttggtaaaa ggggcaagca aaggagaagg attaggaaat
     8641 aaatttcttt cgcatataag agaggtcgaa gctgttgttc atgtagtgag atgttttgaa
     8701 gacgaaaatg taatacatgt tgaaactacg gtcgacccta aaagagatat agaaataata
     8761 gaattagaat tggttttagc cgatttggaa gtaatgtcaa aacgacttga taagctttca
     8821 aagatggtta aaaatgataa gtccctgcag gcagaatttg aattggttca aaaaattgtt
     8881 caagggcttg aaaatgaaaa acaggcacgt tacctaagct atactgacga ggaacaaaag
     8941 cttttaaaaa cttttaattt gataacctca aaaccggttt tgtacgtagc taatgttgat
     9001 gaaagctatc tttcagaccc ttcaggcaat aaatacgtgg atatagtaaa ggagtatgct
     9061 tctcgggata attcggaggt aattgttatt tgcggaaaaa tagaagaaga aatatcggtt
     9121 ttagatgagg atgaaaagaa agaattttta gaggaaatgg gactaaaaga gtcagggctt
     9181 gataaactta ttagagcaag ctaccgctta cttggactta taagcttttt tacgacagga
     9241 ccaatggaat caaaggcttg gacaattgta aaaggtacca aagctccaca agctgcaggc
     9301 aaaatacact cggacatgga aagaggtttc atacgtgcag aagtcacaaa atacgatgat
     9361 gttgtaaaat taggctcaat gcatgcggca aaggaaaaag gattgatgcg tcttgaaggt
     9421 aaagattatg taatggaaga cggagatata gtattattca gattcaacgt ttaattgtca
     9481 tttttcttta caagagagaa atacataatt atttgttgtc ttatatttga aattactaaa
     9541 aagctgcctt ttcggcagct tttggttatt acaatcctaa ctcttctttt aacttactta
     9601 tttcatcgtc tactgaagca ttgcccattt ttgcatattt ttcttctaga gcttttgcct
     9661 catctatggg ctccatattc agctctgcca ttgcatttgc ttcgtctaac atcctgtttg
     9721 ctttgtcttc catacgctca aaagcacttt ttgcaccggc tattttcttt gttgcctgag
     9781 tggcttcatt tagtgtttcc tgtgttttag ctactttaac ttttgatttg ataacttgtc
     9841 gccttgctct taatgtttca atgtctgcag ttaacttatc atgcatctgt cgcattttaa
     9901 ttgcattttc atgagcagcc gcataagcag tcattagtcc tgctcccgca ttttccagtt
     9961 cttgtttttt gttaataaat gttcttgcat catcctcatt gcctgcaact aatgctttct
    10021 tggcaaattc gttatatttt gcaacttctg cctcattttg ctcaactaat ctctttgtac
    10081 gtgtttcttc tgctataact tctgctgtgc tttgttttac ctctgcaaga tctttcatca
    10141 tatcttccaa gtactggtca atcatttttt ccgggtcttc cattttttcc agcaatacat
    10201 ttatgtttgc cttgataata tctccaaatc tttctaatat agccatttct tcctcctttt
    10261 aatttaaccc tgccttttgg cagattattg ttcattatca tcctgatatt ttttcatcag
    10321 gtcttccgct tctgttgtac caaacctttc aagaggggta tttagcattt cttcccttct
    10381 ttttgcttcc aaattttttt gctctttatg ctttttccac catatgaaaa gtacaataac
    10441 taaaacaact acacctaata caataaacac cgtaatccat ggcgacctgg ttaccgtcat
    10501 tattctgtca gctgcatcag tgaaagcttt gctgaaaaat tcttcattgc tcaaactgct
    10561 atcataataa tatctgtcta tataatcagc caaaatatta ccggcttcag catcaattac
    10621 actttttgcc tgtgttccga cgatgtacca gcctttgtac tgaccttcat attcaaaaaa
    10681 tactataagt aaatgtgctt catcagtaaa caactcatca tataaatcag tcgcaaaagc
    10741 ctctaaatct tgctcggtcg gaaaatgaga accgttaatc gtatccgtca aatataaata
    10801 tggctgaact cccgttttct cataaaaatg ttttaagccg ctgataagtt ttgtttcatt
    10861 attaatccaa cccaggcgat ccgtatagta ctccgtttca tttacagacc cctttgggag
    10921 cggatctctt ttaacagatg aaaaagtaat gtcgccgccg ccgcttgagt tcatggagcc
    10981 ccataatgaa aaaattacaa taaaaataac cgcaatcaca attaaaacaa caattaatgt
    11041 aacgcatccg cagcctccgc caccgcttga cctctgcggt ctaccgccgc cgaatcctcc
    11101 gcccatgggg attctgggac caaataccgg accaaatccg gtaccggtac ttttactgct
    11161 gccaaatatt cctccgctgc ttgagcctct gaatgagccg ccgctgcgtc caccaaatga
    11221 gccgccgctg cgtccgccaa aagacccgcc gccacggcta ccgccaaatg aacctccccc
    11281 gcgactaccg ccaaatgagc cgcctccacg gccgccgccg ccgaatgaac ctccgcctct
    11341 agccaatgaa atctcctcca attatatcaa agatactgta ttaattttta tcataatcat
    11401 caagaaaatt acggtacaca ttcccttttc gatagccgat aattgtatct aaattcacat
    11461 tgtgaatgct cttaaatata tttgtatcga tattcggatt gatttgtctt aacttattta
    11521 ttaatatttt catttcctgc cttttagaac ctgtaccctc agccttaatt ctttccgtaa
    11581 tgcggcaggc acattgaata aactgcaaat tattgtatct tttccaagca attatatcat
    11641 cttcccggac tttatacata ggtcttatca gctccatacc ctcatgattt gtactgtgaa
    11701 gtttgggcat cattgttttt atttctgcag cataaaataa gctcattaga actgtttcaa
    11761 ttacatcgtc aaaatggtga cctaaggcta ttttattgca gcctaattct cttgcatttc
    11821 tgtacaaata acctcttctc attcttgcgc ataaataaca cggggtttct tcaactctgt
    11881 caactatttc aaatattggt gtgttaaaaa tttttattgg gatatttaat atttctgcat
    11941 tttcaatgat acgttttttg ttagcatcat tgtatcccgg gtccattacc aaatattctg
    12001 tttcaaactg aatttcactg tatttttgaa gttgctgcat gcatttagcc ataagcattg
    12061 aatccttgcc gcctgatatg cacactgcaa ttttgtcatt gtcttggatt aatttatatt
    12121 cttttattgc tgatataaat tttgcccata ttaattttct aaaccttttt gtgatacttc
    12181 tttcaatttc ctcatattta tccattttgt cctgcttaat aatatgatag taataataat
    12241 agttatactc tattatatta acataatcta cataaaacaa gatattttta tattgcattt
    12301 catgaaaatg tttgctatcc taatattgga tttaggggtt aaatactttt taaacaagga
    12361 ggatttatgg gaatagctca cgaggtggat tataagattt acggagacga tattcagttt
    12421 gtagaaatcg gtctcgatta cggagaaact atcgttgcag agcccggagc aatgatgtac
    12481 atggattcaa gcattgaaat ggatactatg tttggagatg gaagtgaaaa aagcaagggc
    12541 tttaagggaa tgttggcaac agcaggcaaa agggttctta ccggagaaaa tttatttact
    12601 gcaacattta ctaacacgag tattgataaa aaggtagttg cttttgcagc accctatccc
    12661 ggaaaaataa tacctgttaa tttgcctgat tacggcggac ggcttatttg tcaacgagat
    12721 gcctatttat gttctgcaaa agggattgaa ataaccattg cattccagaa aaaaatcggt
    12781 gcaggacttt tcggcggtga aggatttata atgcagaaat tggttggaga tggttttgca
    12841 ttccttcacg caggcggcac aattgttaaa aaggaattag cttcaggaga aatgcttaaa
    12901 ttagatacag gatgtttggt ggcatttact gaaggagtag actatgatgt tcaatttgca
    12961 agcaatgtaa aaagtgcctt gtttggaggt gaaggtttat tcctggctac attaagaggt
    13021 cccggaactg tatggatgca gtcattgcca ttcagcagaa tagctgacag aatttattcg
    13081 gcaagcagaa aaagcggcgg aggcggctca aaaggagaag caagtttact tggaaatatt
    13141 ggaataggta acttattcgg tggtgattaa agatgcccta accctatttg ttagaggtat
    13201 ggggatacac attttttctg aaggtgctca gcgagcacct tctttttcat tcttaaatat
    13261 attacaaact gccttggttg ttgaaatatc ttcttctatt atttcataag agtccagcct
    13321 tttttttatt tcatccttat atgctgaaag catgatagag ctgttgtgga aaactcctcc
    13381 ttttagacct atattagcag ttcccttaaa atttttcttt ttatacgcca ctgatgtaag
    13441 ctcagcaaga tatttgcctg cattctctaa gagcatggtt gcataagcat ctcctttctc
    13501 agcataatct gaaattatgg gaaaaagcga tgctatttta tttttttcat tattatatat
    13561 aaaattcatt atatccgagc gtgtagagcc gcctatttct tccataacag ccttgcttaa
    13621 gtcatcaaag ggaagagccc tgtcatattt ataaactata tttttaataa catttataac
    13681 cgtatgataa ccgcttcctt catcgcccaa aatatgtccc catcctccaa ccatttcacc
    13741 tgctccgtct ttttgagcaa agcaagaaga ccctgttccg gaaattgcca gaattccgtc
    13801 cttatctcca aaatatgctt tgcaagccat ttcggcatca ttcaatacta tagtcttaat
    13861 tacaaaagca ccttcgacat attttttaat cagctcagca tagtttcctg tttcaacacc
    13921 tgccattccg acagctataa gctgacattc ttctcttcct aattcattaa cgcagtcata
    13981 aattaatgaa attaagttgt tgacagtctt gtccaagttt accgcaggat taccaaattc
    14041 tcctattaag ttgaaaacct gagcattatt aaggtcataa gccgtaagct ctgactttgt
    14101 tccgcccgaa tcaattccaa ttaaatattt catactctca ccacccgata ttagtatagc
    14161 aaaaactact ttatttttcc atataaatta taaatattat taattattta tagttaaaac
    14221 aactcatcaa taaagattat atgaaataat aaaactgctc ctctaatcta ggggagcagt
    14281 cttgcataca tttattccac atgaattgca tctgtagggc actcttcttc agcttctttt
    14341 gcacaatcct cgaattctga aggtacgggg tcaaccttta ctacagagat accttcatcg
    14401 tccagttcga aaacatccgg gcaaattgat tcacacagcc cgcagcttat gcaagcatct
    14461 cggtcaacat tagctctcat aatttcctcc ttttaagcaa ataatatttt attattaatt
    14521 cattttaatt cttttggtgt cgcattaaat attatctaat gcttatattt ccttgtagct
    14581 gccttatatt taaagaaccg ttagcgatac tctaatattg ttctaaaaat taatatattt
    14641 attcattatt gaaattttca ataatgatac attctataag cttagattta cagcagttta
    14701 tccaaagctt gttttaaatc atcaattata tcgtctgcat gctccaaacc aaccgatagt
    14761 ctcacaagac cttcagatat tccgcttaaa gccctttctt cgggagtata aggtgaatgg
    14821 gtcatggaag caggatgctg aatcaatgtt tcggtatcgc ctaagcttac tgctaatgag
    14881 caaagctcaa cggtattcat taatttcctt cctgattcaa gacctccctt aagttcaaat
    14941 gcaatcattg ctcccggcaa agacatttgt tttttggcaa gttcatattg cggaaaactt
    15001 tttaatcccg gataacctat ccactctatt gcaggatgag actcaaggaa ttcagctacc
    15061 ttttgggcat tggagcagtg tttttccatt ctgacttcta atgttttcat tcccctgtta
    15121 atcaagtatg catcaaaagg actcataacc gagcctgtca tatcttttat tccaaatagt
    15181 cttaccttgt caataaattc ttttttaccg gctgcaaaac cggcgataac atcgccgtgg
    15241 ccatttaaat actttgttgc agaatgaaca accacatctg caccaagatc aattggtctt
    15301 tgtatataag gtgtgcaata agtattatcc accacaacca tacagccttc tgtctcgtga
    15361 gcgattttag aaattaccga aatatcggac aaaagcatat tcgggtttgc cggactttcc
    15421 aagtatacaa ttttagtgtt aggcttcata gccttcctaa cattttcagg atctgaagta
    15481 tcaacaaaat ctacttcaac accgaatttt gtcaacccat ggctcatgaa agcaaacgtg
    15541 catccatata atgtcttagc cgcaacaata tggtctccag cacttactat agaccatata
    15601 acggatgtaa tggctcccat acctgaagcc attgcaactg cagcttcagc attttcaagt
    15661 gaagctaatt tatcttcaac ctgagagcat gttggatttc caagtctggt ataaatataa
    15721 ccttcttctt ccaatgcaaa tcttcttccg ccttgttctg ctgaatcaaa tataaatgta
    15781 gaagtttgat atattggtgt tgctagagct ccatactcgt ttggtctgta accggcgtgt
    15841 accgcccttg tagaaaaatg cattctactt aacttgtcct tatccatatt tcctccttat
    15901 aaaaaatgac gataagtaaa cgtttctata tataaattgt atactacttt taaatataaa
    15961 tcaatccata tttatcaaaa agttgtccat tcatttaaat ggtaccgtcc actcatttaa
    16021 ataacaaagt gcatcatata aaaaaatttt gcccccggaa aagcggtttt ataaggtatt
    16081 ttacttaaat tcatcaaaca ccaagaggta ataagtggac agcaaattat tataaggggc
    16141 tgcaggatat aatttaatgt tgcctaattg gattttgctt gattttttaa ggaatgatta
    16201 tataattata ttaaatatat ttaatggaga ttttaatgga ctttataata gcagtcataa
    16261 tttttatctt ttctcttata tataacattt cgaaacaata ttccttaatt attcctttac
    16321 tgattggtat gcttgctttt tcttccgttg ccttttacag gggatttaag cttagaaaca
    16381 ttgttgtaat gttaatgaag ggcatgaaaa agtcactgta tatattgtct atttttgctt
    16441 taataggaat gattacagct ctgtggcgtg ccggcggaac aattccgttt tttgtttatt
    16501 acggcataaa aattatgaat ccggattatt ttatattatt tgcattttta cttacatgtt
    16561 tcgttgcatt tgctcttggt acatgcatag gaactgccgg aacagtggga gttgtattga
    16621 taatattggc cagaagcggc ggagtaaaca tctatgttgc agcaggcgcc attttatcgg
    16681 gagctttttt tggagacaga agctcgccct tatcctccag tgcaaattta gtagcgatta
    16741 ttacggaaac agatttgtat gataatataa aaaagatgtt tattacaggc gctctgccat
    16801 ttctcgcaac tatagttgtc tatttgttat tatcaaaaaa taatcccata aatgtcacaa
    16861 ataatgaatt gatagagaaa ttttccgtta atttcaatct gcatatatca acagttcttc
    16921 ctgccgtcat aattctcttg ttggcaatat ccaaaaatga cgttcgggta gcaatgcttt
    16981 ttagtataat aactgctgtc tttatatgcc tgtatattca aaatttgacc ttgcaggaaa
    17041 ttttaaaata tatgattttt ggttttgtca acactgaccc tgcgctttcc gaaattattt
    17101 cgggaggcgg attaatatca atgctcaacg ttacatttgt cgtattgata gcatcatcat
    17161 attccggcat atttgaagga accggcatgc taaaagatat tcagggagct ttaataaaga
    17221 tgagcaataa aataggtgtt tacttaacaa cagtctttac aagcctcgtc acttgtgctt
    17281 gctgctgtaa tcaaacactt tccgtattgc ttacccacga cttaatgaat aatatttata
    17341 aagaaaataa ccttacaaaa gataatttag ctattgatat tgaaaatacc gcaattctta
    17401 ttgctgcgat tttcccgtgg agcgttgcgg tagctgtacc gcttgcaacc atggacgttg
    17461 gcgctaaagc aatattctat gcagtatatc tgttctttgt tcccttaatg gctccattga
    17521 tacccaaatc caaggtttac aaccttgggg acggaaattg atatacaatt aaaattatcc
    17581 cgtccccggt gcccctatag ttttttgatt tggaaattat cagcaagcaa ggggaagttt
    17641 tgagggaatt catatcctaa cccccagtag cttattcctc ttaaaccgta ttgtttcaca
    17701 gtatcaaact ttgcctgtgc acttcttgca tcttcaaacc aaactacatg agaatttcct
    17761 tcagcatccg tataataaaa atacggtgac tgacttacat aatcataatg aatttcagac
    17821 atatgctcat atgctaaatt atatgcatcc tgaacgctga ttgtttcagc ttcatcgcct
    17881 tcttgaaaag gaattctcca gtctcttgca tatatttgaa atcccatcaa tattttatct
    17941 ctcggaatga cagttactgc gtaatccaac acccttctta tttcatttat cggagaaatg
    18001 gctcttggtt ctcctcccct ccagccccat tcgtaggtca tgagaacgac aaaatctgcc
    18061 aatcttccat gagccggata gtcgtgcgct tcatataaaa gccctacttg ctctccgctt
    18121 attttaggag ctaatgatgt ggaaacaaaa aatccttcat tatgcaatct gttaacggtg
    18181 ctttccaaaa aactgttgta ggcttccctg tcttccggaa gaacgtattc aaaatcaatg
    18241 tttagtccct gatatccttt ttctctcata atatttataa tattttcctg taatgtatca
    18301 atcgtatcag gattattcaa tactatgttt gcaagatttt ctccttccac attgactgta
    18361 aaatttacta ttgccatcat gggcaccacc ccttgtgaat aagcttcagc tattgccggc
    18421 tcatcggcaa tattcaccaa ggttccgtct tcattaatca ggtatgcaaa agggctcaaa
    18481 taagttaaat agggtcctgc tcctcttact atcggaacag cttcgcttcc aagaaaatat
    18541 gtaaatgcat ttacatctat ttcaggctta ggcctttgag gtattgtaag tacattaccg
    18601 ggatatatca aatcctgatt aatatttctg ttttccgcca gtaagtcttg gatattaacc
    18661 ccaaactttt gcgctatttt ccaaatcgta tctcctcgtc taactgtata ggtatttttt
    18721 gttggtataa gaagtgcatg gccgggcagt aaaatatttg gattactcaa tttgttaaga
    18781 tctacaatat cttgtatgct gacattataa cggtctgcaa ttttccacaa tacatctccc
    18841 ttttgaacaa catgaatata catagtttcc ccctttattt ctttgttatt ataacatgaa
    18901 actgtaatgc aattttaatg acaatacccc cgtccccttg tcatatatat atctcttcat
    18961 gttataatat attcaattgt ccacctataa tgataaaaat aaatgttgcc ggagagtaat
    19021 ttctccggca acatatctga caaggggacg g
//
LOCUS       DAOSHP010000031        18955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2360_length_18955_cov_8.939947, whole genome shotgun sequence.
ACCESSION   DAOSHP010000031 DAOSHP010000000
VERSION     DAOSHP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18955
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2360_length_18955_cov_8.939947"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLB33_04670"
     CDS             complement(<1..464)
                     /locus_tag="PLB33_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOG62849.1"
                     /translation="MENKTLENLMAAFAGESQATRKYEIYSAIAEKEGKHNAAKLFKA
                     AADAEKLHAKKEYELAGKVGKTADNLVDAKAGETYEYQSMYPEFIKVAEAEGNKAAVG
                     AFTFAMKAEEVHAKLYQEALDNLDETEEVFYYLCPVCGNIEKSVPDKCAICGV"
     gene            complement(484..903)
                     /locus_tag="PLB33_04675"
     CDS             complement(484..903)
                     /locus_tag="PLB33_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOG62850.1"
                     /translation="MKTLKELSDELKKNNIRLTHQRLKVLEYLSNSKNHPTVEQIYNA
                     LKKEVPSLSKTTIYNTLNYLSELNLIQVLAIDENEAHFDAVTNTHGHFKCKTCGKIYD
                     FNMDMDTLRVKELDNFKITEKLVYFKGICERCLSNTN"
     gene            complement(1060..1332)
                     /locus_tag="PLB33_04680"
     CDS             complement(1060..1332)
                     /locus_tag="PLB33_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62851.1"
                     /translation="MPINKNVHEQMRNWELQQKQKSQKELREKYEYMNDKYQQMKNSY
                     SGFRNVHKDDNDDAIFDKSSDLADDNSYMRYELNNTFKNKDDKKNK"
     gene            complement(1542..2438)
                     /locus_tag="PLB33_04685"
     CDS             complement(1542..2438)
                     /locus_tag="PLB33_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG62852.1"
                     /translation="MTKLKADLALLMVTFGWGISYYLTDLSLEDMGPFTLNSNRFLTA
                     FAVAVILAFPKLKNISKRTVKFSFLLGVVLVLVYLGATFGTLYTTLSNVGFLCGLTVI
                     FTPILSSIIYKKLPGKKQTFVILLSFIGIALLTLKDDFSINYDNLLGDLLCIMCAVSY
                     AAHLLITEKAVTFEDVNPFHLGVLQLGTTGVFTLILAFIFETPHLPTQPKYWGPVLFL
                     SIFCTGVAFIVQCVAQQYTEASHVGVIYSLETVFAGIVAFALANDVLSFQSYVGAALM
                     VASIFIMEIDFSKIKIFQKQKQ"
     gene            complement(2669..3700)
                     /locus_tag="PLB33_04690"
     CDS             complement(2669..3700)
                     /locus_tag="PLB33_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG62853.1"
                     /translation="MISIKELEMKKIKDLEAQYPFILSFFENNKLNVEEFKDSTLIEY
                     LNHFTEEEIEEWAIDISKIKSDLETYINQMLQFLGLEKDIVDSITILAGKDKYGNKEN
                     FDELTINKSEIVSIVGPTGSGKSRLLADIEWAANRDTPTERAILINGEKPDYSIRFST
                     GNKLVAQLSQNMNFIMDLTVKEFLELHAKSRMIENEQEVVSKIVEAANELAGEKFNLD
                     TPVTGLSGGQSRALMIADTAILSTSPIVLIDEIENAGIDRKRALNLLVNEEKIVLMAT
                     HDPSLALMADKRIVIKNGGIYKVIETGSKEKEVLKELEKMDKIINTLRFKLRSGDTID
                     TDFGEEMIS"
     gene            complement(3687..4394)
                     /locus_tag="PLB33_04695"
     CDS             complement(3687..4394)
                     /locus_tag="PLB33_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG62854.1"
                     /translation="MNLVIISGPPSSGKTSVILKTIEAIKKRGIKAGVVKFDCLYTDD
                     DILYEKAGIPVKKGLSGSLCPDHFFASNIEEVVRWGNKEGLDLLITESAGLCNRCSPY
                     IKNIKAVCVIDNLSGINTPKKIGPMLKTADIVVITKGDIVSQAEREVFASRVNSVNPQ
                     AVIMSVNGLTGQGAFELSTLIYDENLNVETVKGSELKFPMPSALCSYCLGETRIGEQY
                     QMGNVRKMNIGEENDKH"
     gene            complement(4391..5581)
                     /locus_tag="PLB33_04700"
     CDS             complement(4391..5581)
                     /locus_tag="PLB33_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG62855.1"
                     /translation="MSIDIKDTLFNITEKHKEALDLLISLGFDSLKDDNLRKVFGSSI
                     SLENALKSKKINADEFVTRLMERIEENKSAQKKFKDTIRITGVLPCPVKVPLMETFEK
                     WLSEQNFDFNLNYDLKAASMGIDWIKDEMKNSVNVPDLFISAGFDLFFDKNLLGKYKS
                     ENLFEDITGIQKYNDDFSEYNLEDPSGQYSMLGIVPAVFLVNKDELKGRKIPQSWEEL
                     MSGEFENNVSLPISDFDLFNALLLNIYRLYGEEGIRKLARIFQKNMHPSEMIKSHMKR
                     ERPAITIMPYFFTWMVREGSPMELVWPKDGAIISPIFMLTKKETKDKTKTIADFFASK
                     EAGEILSHKGKMPSVNPEVDNQIPKENKYIWLGWDFIKENDISNLIKKCENIFNDTLK
                     EMTK"
     gene            complement(5760..6617)
                     /locus_tag="PLB33_04705"
     CDS             complement(5760..6617)
                     /locus_tag="PLB33_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOG62856.1"
                     /translation="MYGWMIYPKNTINNKFGNNAFDWMEESALKNGISIDIVFSDDLI
                     ILNNNSKTEFIYSGKSLKLPDFVIMRDYNYTISMQLENLGIRVINSTLSMFNSRNKAV
                     TAQLLVKNNVSTPKFLITKEKNFKFIHNYFDKRKFVMKENEGSQGKGVYLIENEEDYN
                     NVYHKIKDEYFCQEFIESSYGMDIRVYVLGDKVLGCVKRISDSGFKSNYSLGGRVEKY
                     EMTDSIKEISLKAAKAIGLDFCGIDLLFAKDSFTVCEVNGNAGFRTITQVSDIDIPME
                     LFKWVKESL"
     gene            complement(6676..8496)
                     /locus_tag="PLB33_04710"
     CDS             complement(6676..8496)
                     /locus_tag="PLB33_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62857.1"
                     /translation="MKEKQLKPKDPKKTFLRLFSYFKYNLVLFFGGIFFIIVGSSAEI
                     GINGMISPVIDALIGDNDSGLFVKYLLIMVALVVISSISQYIGNLSMAKLAQKTVYKI
                     REDMFSHMEKLPVSYFDKHSHGELMSTFTNDVDMLNQSLEQSASQILISFVTVVGTFT
                     VMIILSPILTAVVAAMLVVMLYSIKFVSKRSAKNYRMQQAALGDMNGYTEEMMSGQKV
                     VKVFNYEDRAIDTFSKKNEELRNASTLASTYGVMLMPIMGNLSFVMYAIISMLGAYLV
                     MINQLSIGNIASFLLYTRTVSRPITMVSNQLNSLFAALAGAERIFNVLDEKIETDEGD
                     VTLLKDCTGKKALCWMVPKGDGDYEKVPLKGFIEFKDVNFGYSPEKRVLNKIILYAKP
                     GQKIAFVGSTGAGKTTVTNLINRFYEINEGEILYDGIDIKRINKHDLRSTMSIVLQDV
                     HLFEGTVKENIRYGRLDATDDEVVEAAKLANAHYFIKHLPDGYDTVLSSDGQNLSQGE
                     RQLLSIARAAIADPIILILDEATSSVDTRTEKLIAEGMDKLMAGRTTFVIAHRLSTVR
                     NANAIMVIEHGEIIERGDHDELMAQKGRYYALNAGTAELS"
     gene            complement(8493..10214)
                     /locus_tag="PLB33_04715"
     CDS             complement(8493..10214)
                     /locus_tag="PLB33_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62858.1"
                     /translation="MKKLLPFMKKYIKFAVLSPILMILEVIADIAIPYLMSRIVDVGI
                     ANRDIEYVIKIGLIMIAAALLAMISGVISSHYGARAGFGFASEVRLKLYKKIQGFSFA
                     NLDKFTVASLITRLTNDCNTLGQVTMMSLRMAVRAPFMMIFALLMTIRINASLTKVFA
                     VSIPVLVIALIIILSKARPLFLKFQTRVDRVNAIIRENLTAIRVVKSFNRQSHEEKRF
                     KERNDSLMLTALKAITLIVFLMPVLNIIIYSTIIAVLWFGGKQVVAGSMGSGELISFI
                     TYITQILMSFMMMSFFFMQLLRASASVTRIAEIIDTESEIKEVKNPVKELADGSVTFK
                     NVCFCYPGSTEKTLKNINLHINSGEVLGIIGSTGSSKSTLVQLITRLYDATEGSVIVG
                     NVDVKHYSIKTLRDNVAFVLQNNTLFSGTIRDNMKWGNENANDEDIIRALKLAQAWEF
                     VSTYEDGLDHKVEQGGDNFSGGQKQRLTIARALLKSPKIIILDDSTSAVDMATDAKIQ
                     RVFKEELNDVTTIIIAQRVSSIQHADRILVMNEGEIESIGKHEELLVSSPVYREIYES
                     QQKGVGV"
     assembly_gap    10252..10351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10352..11990)
                     /locus_tag="PLB33_04720"
     CDS             complement(<10352..11990)
                     /locus_tag="PLB33_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62859.1"
                     /translation="MRKASEKQRAWNIIWSVSENYSITPDITAYDEKGKADLYWNYII
                     GSVYKYYDYNLLREFFDTLRYDRDYIFYQKVLLLGLEGCIFEKDSKNRPVLAGLRFDY
                     GKKAYEKREEAEVFDEISAAYFSQVFGYDMKVRDNVKSLINELHFDINFNTEQVINKL
                     KQIINKYFEPEYSSEFYIKKIKDNMLLRFFGRKSPSSSLMLIDTDEGRTGTGKIASGW
                     FKFKEKSDNKEREYIKDYFGISAIAESKTKELEKILCDEAHESCHLHFTRGQFDENDD
                     AIFRQKQTSEQRDKNKQHYIKNYIRNTNNITKLTQRIKNAMIVNYTYSMKTESGKLVT
                     EKAWRTVLDDYKIFVRSIKDEIPDMSVDIMLDASASQLNRQEVIAEEGYIIAESLTRC
                     NIPVRIYSFCNMRDFTVMNLFRDYDEKNNNDKIFNYYASGFNRDGMALRTALHMMKDS
                     KYSHRILIVLSDGRPNDMLGVTSKGIIPLQKEYSDETGVSDTANEVRKGRKQGVSILC
                     IFTGRDDDIPSVKKIYGQKFTRILKLDKFAEMVGVLIE"
     gene            complement(12072..12383)
                     /locus_tag="PLB33_04725"
     CDS             complement(12072..12383)
                     /locus_tag="PLB33_04725"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HOG62860.1"
                     /translation="MFDENVIKITERAMKHLKKKGKLQITIEIPENRLSSESVLVPIP
                     EIIAKKPKAPENYQIINIGGIDVYISRTLVLPKNDVVIDLDTFLGIKLLRVSGFKSNE
                     G"
     gene            complement(12465..13286)
                     /locus_tag="PLB33_04730"
     CDS             complement(12465..13286)
                     /locus_tag="PLB33_04730"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOG62861.1"
                     /translation="MYIEIIKAIILGIVEGITEWLPISSTGHMILVEEFIKFNASDEF
                     MEMFRVVIQLGAIMAVVLLYFHKLNPFAPSKTPKQKRDTMDIWFKVIVGVLPAAILGF
                     LLDDWFDENFYNYQTVAITLIVYGILFLIIENKNKGKPAKIKSFKELSYKTALITGFF
                     QVLSLVPGTSRSGATILGAIILGTSRNIAAEYSFFLSIPVMFGASALKLVKFGIDFTG
                     LELAVLLTGMTVAFIVSVIAIRFLLRYIKNNDFKAFGWYRIILGILVVGYFMLIS"
     gene            complement(13489..14961)
                     /locus_tag="PLB33_04735"
     CDS             complement(13489..14961)
                     /locus_tag="PLB33_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62862.1"
                     /translation="MKNKISIKLIVYFSAALIVFSIIIGNIFMLLFKNNAVEIHKNEL
                     KRRALTIADTISELMDGSSLNFGPGMGRHGGMMGMQGGLGLYIRNLDDIAMADAWIVD
                     EELNLLITGSMSHIQYNYTDLPQDADIVVKKVFEGQTTFSEGFSKLLETPALTIGTPI
                     TSDGEIIGALLIHSPIQGINDATSEGFRILLISIFMALILSVLLSVALALTFTKPLEK
                     MKNTAALLAGGNYSVKTGIKQNDEIGDLAKTMDILSQRLGKAEEERKRVQKQRQDFIT
                     NISHELRTPVTVIRGSLEALSDGVITDSDKINEYYKQILIESKSLERLINDLLELSRL
                     QNVDFKIEMQELNICDVLKDSLRSASNLARQKNINIQYNSDKDVFLTKGDYGRLRQMF
                     LTVIDNAIKFSRENKSIYITLKDNQITIRDEGIGISEKDLPNIFDRYYSVKSDENKTG
                     TGLGLTIAKQIALRHDIELFAESKINEGTSFVFVIRSNFE"
     gene            complement(14951..15640)
                     /locus_tag="PLB33_04740"
     CDS             complement(14951..15640)
                     /locus_tag="PLB33_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG62863.1"
                     /translation="MPKMLIADDNRQITSILEEYAKKEGYDVKIAYNGRQALDTFDKH
                     KPDIVLLDVMMPIIDGFEVCRRIRKVSNVPLIMITARGEDFERIMGLDIGADDYIVKP
                     FSPAEVMARVRAVMRRIGAENISSKLFVYDNIKINMEDYIVTIDENNVSLTKKEIEIL
                     WTLAVNKNKVFSRENLLNSLWGYDYYGDSRTVDSHIKRLRSKIDNFQHKNWEIKTIWG
                     VGYKFEVYDEK"
     gene            15808..16194
                     /locus_tag="PLB33_04745"
     CDS             15808..16194
                     /locus_tag="PLB33_04745"
                     /inference="COORDINATES: protein motif:HMM:NF022378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HOG62864.1"
                     /translation="MKNKILIIGLVLLIILATSITVFAITGRTQTAYDESEDTWEKFR
                     DEMFENKKAFIEERVKDGTLTQEEADEILKNIELMQEYCLGVGGCGMGRGFGMMRNRL
                     GNNSGYGNYGFRGMGFGGGRCGRGSW"
     gene            16312..16944
                     /locus_tag="PLB33_04750"
     CDS             16312..16944
                     /locus_tag="PLB33_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG62865.1"
                     /translation="MYNYYNYPCQNIYPWYRQQDRMYNRQRMYNPYNNLMNPDFNNYP
                     RYPTYPDYPYNQESFYSPWSLQYPSDRGFIEIKDYGPNPFVINIEEATEQNNTFRSTL
                     WTGDHLQLTLMSLNPGEDIGLEMHPDVDQFIRIEEGRGIVQMGDSEDNLDFEEMVSED
                     FIFIIPAGKWHNLTNIGRTPLKLYSIYAPPEHPHGTVHETKAEAEAVHGN"
     assembly_gap    16991..17090
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17095..>18955)
                     /locus_tag="PLB33_04755"
     CDS             complement(17095..>18955)
                     /locus_tag="PLB33_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62866.1"
                     /translation="TVGGTFNYINFDSMPSNGKIYIDYVSSTNKGTAVEAGKEYSKAD
                     LDKMSFVPNSGYFGKFTIPYKGYNTAGDVFTGKVEITVAEKVFVIDKIKYSLYVNTLV
                     DFNADDFEEVCLDGTGQRLNYVKFTLPSSSRGTLYSNYGLSSQSKVSSTSKYYDDDID
                     DISFVPYKNYSGTVVISYTGYNSKGESYTGEIEIQVLKVVTDADDITYKTGSYTPIDF
                     DADDFDDECRDVTGKRLDYVRFTLPSSTYGTLYYNYSSRSQSKVSSTTKYYDDDLDDI
                     TFVPKPSYTGTVVISYTGYNIDGKSYTGAVKITVTKEVPVADDIKISTKENTPIRFDD
                     EDFNDASEDSTGVELDYVTFTLPSSKYGKLYYNYTSSSNYGSEVTSSKKYYYEDTPSL
                     YRVTFVPYKDYSGTVTIKYTGYNTDGFSFTGSVKVEVVAAPKTEGSLYFKDVTKDYSW
                     AASQIDYLFKEGVVAGTGNNNYSPAQNVTRGDFMLMLYRALDLTATDRGNFSDVPKDS
                     YYYKAIAVAKSLGIAKGYGDKFMPTAGITREDAMVLVDRALIIENKRLSAGKESDLYA
                     FKDRNSVSDYAVTSVATLVKAGIIQGNNSYLYPKSMISRAEMAVILYKVLQLD"
BASE COUNT         5572 a         3697 c         2841 g         6645 t
ORIGIN      
        1 actccgcaga tggcgcattt atccggaact gacttttcta tgttgccgca aacaggacat
       61 agataataga atacttcttc tgtttcatcc aagttatcca atgcttcctg atataatttt
      121 gcatgaacct cttcagcctt cattgcaaag gtaaatgctc ctacagcagc cttatttcct
      181 tcagcttctg caactttaat aaattcagga tacatgcttt gatattcata tgtttctccg
      241 gcttttgcat ctaccaagtt gtcagctgtc tttcctacct tgcctgccaa ttcgtattct
      301 tttttagcat gaagtttttc tgcatcggca gcagctttga ataattttgc cgcattgtgc
      361 tttccttcct tttctgctat cgcagaataa atttcatatt ttcgagtagc ttgtgattct
      421 cccgcaaatg ccgccattaa gttttcaagt gttttgtttt ccatttttaa ttcctccatt
      481 aatttaattt gtattagaaa ggcatctttc gcagattccc ttaaaataaa ctaatttttc
      541 agtgatttta aaattatcga gctctttgac acgtaatgta tccatgtcca tattaaaatc
      601 ataaattttg ccgcatgtct tacatttaaa atggccatga gtattggtaa cagcatcaaa
      661 atgagcttca ttttcatcta ttgctaaaac ctgtatgaga tttaactccg ataaataatt
      721 taatgtattg taaattgtag ttttagacaa agaaggtact tcctttttaa gagcattata
      781 aatttgctcc accgtaggat gatttttact gtttgataaa tattccaata ccttcaacct
      841 ttgatgagta agacgtatat tattcttttt taattcatcc gataattctt ttagtgtttt
      901 catgcaaacc tcgtttataa tcatttttat tttgtaatga ttacaatgta atgatatagt
      961 attatttatt tgatgtcaag agaaaaatta aagattttta aataataaag gcaaatactc
     1021 atagtattga atatttgcct tatttgcctg taaaactaat tatttattct ttttatcgtc
     1081 cttgttttta aaggtattgt ttaattcata tctcatataa ctgttatcat ctgccaaatc
     1141 actgcttttg tcaaatattg catcatcatt atcatcttta tgaacatttc tgaagccgct
     1201 gtaactgttc ttcatctgct gatatttatc attcatatat tcatattttt ctcttaattc
     1261 cttttgactt ttctgcttct gctgaagctc ccaattacgc atttgctcat gaacattttt
     1321 attaattggc ataatatcat cctttctttt atattattaa tgatattatt tgtttatcca
     1381 attaaattta ttacaggtat aaaagttaaa tctgcaagat aattacagtt gtaaatctca
     1441 tcaataagca gagattccgg tttaaaatta tcaaatgtct attacattga taatgaataa
     1501 gctttgtaag tatacataat gttatttatg taaactatta ttcattgttt ttgtttttga
     1561 aatattttaa tttttgaaaa atcaatttcc atgatgaaga tacttgcgac cataagtgca
     1621 gcacctacat aggactggaa tgaaagaacg tcattggcga gagcaaatgc tacaattccg
     1681 gcaaacacag tttccaatga gtatattaca cctacatgag atgcttctgt gtattgttga
     1741 gctacgcatt gaacaataaa tgctactccg gtgcagaaaa tcgataaaaa caaaacaggt
     1801 ccccaatact tgggctgtgt cggcaaatgt ggagtttcaa atataaaggc tagaattaaa
     1861 gtgaatacgc cggtggttcc aagctgcaga acacctaaat gaaagggatt cacatcttca
     1921 aatgttactg ctttctctgt aatcaacaaa tgtgcagcat aagacacagc acacataata
     1981 cataaaaggt ctcccaataa attgtcatag tttatagaga aatcatcttt taatgttaaa
     2041 agtgctatgc caataaaact caacaaaata acaaaggttt gctttttgcc gggcagtttc
     2101 ttataaatta ttgacgataa tatgggggta aaaattacgg taagaccgca taaaaaacct
     2161 acattggaca atgtggtata caaagtgccg aatgttgctc ccagataaac caataccaat
     2221 acgacgccca gaaggaaact gaatttaact gtacgttttg aaatattctt caattttgga
     2281 aaggcaagta taacagcaac agcaaatgct gttaaaaatc tgtttgaatt taaggtgaag
     2341 ggtcccatat cctctaaact caaatcggtt aagtaatacg agatacccca tccgaatgtt
     2401 accatcaata aagccaaatc tgcttttaat ttcgtcataa tagctgtccc cttttttact
     2461 tcatgctata ttctaataca tatttttgaa ttaggcaatg aaataaattg ggattccttc
     2521 agcaaagcac tcaaaatgac aaaaaaaatg tcgtcctgag tataacggag gatctcatct
     2581 tttgagattc ttcactgttc tcagaatgac acttaccttt attcatatta ataacaaggt
     2641 tgcaccttta gaatgcaggt gggcgttttt atgatatcat ttcttcgcca aagtccgtat
     2701 ctattgtgtc cccgcttcta agcttaaatc ttaatgtatt gattattttg tccatctttt
     2761 ccaattcttt taaaacttct ttttcttttg aacctgtttc aataacctta taaatgccgc
     2821 cgtttttaat gacaattctt ttatccgcca taagagctaa acttgggtca tgggttgcca
     2881 taagaactat tttttcttca ttaaccaata aatttaatgc tctttttctg tctattcccg
     2941 cattttcgat ttcatcaatc aacactatgg gagaggtgct taatattgct gtatcggcaa
     3001 tcattaaagc tcttgattgt cctccgctta atcctgttac gggtgtatct aaattgaatt
     3061 tttcaccggc taattcgttg gctgcttcga ctatttttga aactacttcc tgctcgtttt
     3121 caatcattct gctctttgcg tgaagctcca aaaattcctt aacggttaag tccataataa
     3181 aattcatatt ttgagacagc tgagcaacta gtttgtttcc ggtggaaaat cttatgctgt
     3241 aatcaggttt ttctccattt attaatatag ctctttcagt aggtgtatcc ctgtttgctg
     3301 cccattcaat gtcagccaag agcctgcttt ttccggaacc cgtaggtcct acgattgaaa
     3361 caatttcact tttatttatg gtaagctcat caaaattttc tttattgccg tacttgtcct
     3421 taccggctaa aatggttatt gaatcaacta tgtctttctc taatcctaaa aattgaagca
     3481 tttggtttat ataggtttct aaatcggatt ttattttaga aatatctatt gcccactctt
     3541 cgatttcttc ttctgtaaaa tggtttaaat attcaattaa ggtggaatcc ttaaattctt
     3601 caacatttaa tttgttgttt tcaaagaaag ataatataaa aggatactga gcttccaagt
     3661 ctttaatttt cttcatttcc aattctttaa tgcttatcat tttcttctcc tatgttcatc
     3721 tttcttacat tgcccatctg gtattgttca cctattcttg tttctccaag acagtatgag
     3781 cacagtgcag aaggcatcgg aaacttaagt tcgcttcctt ttacggtttc cacatttaaa
     3841 ttttcatcat aaatcaaggt gctgagctcg aatgctccct ggcctgtaag cccattaacg
     3901 gacataatta cagcctgtgg gtttacggaa ttaacccttg atgcaaaaac ttccctttct
     3961 gcttgagata caatatctcc cttcgttata accacaatgt cagctgtttt aagcatagga
     4021 cctatttttt ttggtgtgtt tataccgctt aaattatcta ttacacaaac agccttaata
     4081 ttctttatat aaggtgagca tctgttgcat aagcctgcac tttcagtaat caataaatct
     4141 aatccttctt tgtttcccca cctcacaact tcttcaatat tgctggcgaa aaagtggtcg
     4201 ggacacaagg agcccgaaag tcccttttta accggtatcc cggctttttc atacaatatg
     4261 tcatcatctg tatagaggca gtcaaattta accacccctg ccttaatgcc tctctttttg
     4321 attgcctcaa ttgtttttaa aattacggat gtttttcctg aagaaggagg tcctgatatt
     4381 attactaaat tcatttagtc atctccttta acgtatcatt gaatatattc tcgcatttct
     4441 ttattaagtt tgaaatgtca ttttctttta taaaatccca tccaagccaa atatatttat
     4501 tctccttcgg tatctgattg tctacttcag gatttacgct tggcatctta cccttgtgag
     4561 acagaatttc tcctgcttcc tttgaagcaa agaaatcagc tattgtcttt gttttatctt
     4621 ttgtttcctt ctttgtaagc atgaaaatag ggcttattat tgcaccgtcc tttggccata
     4681 ccaattccat agggcttcct tctctcacca tccaggtgaa gaaatagggc attatggtaa
     4741 ttgccggtct ttctcttttc atgtgagact taatcatttc agaaggatgc atattttttt
     4801 ggaatattct tgccagtttc cttatgccct cttctccgta cagcctgtaa atattcaaca
     4861 atagagcatt gaataaatca aagtcgctta tgggaaggct cacattgttt tcaaattcac
     4921 ctgacattaa ttcttcccag ctttggggaa tttttcttcc ttttaactca tccttgttta
     4981 cgagaaaaac ggcaggaact atgccaagca ttgaatactg accgcttggg tcttctagat
     5041 tgtattccga aaaatcatcg ttatattttt gaattccggt tatatcttca aataaatttt
     5101 ctgatttgta ctttcctaag aggttcttgt caaaaaataa gtcaaaacct gcagatatga
     5161 acaaatccgg aacatttacc gagtttttca tctcatcttt tatccagtca attcccatag
     5221 atgctgcttt taaatcataa tttaaattga aatcaaaatt ctgctcacta agccattttt
     5281 caaaagtttc catcaaagga actttaacag ggcagggaag cactccggtt attctaattg
     5341 tgtctttaaa ttttttctga gcacttttat tttcttcaat tctttccatt aatcgagtta
     5401 caaactcatc tgcattaatt ttcttagatt ttaatgcatt ttccaatgag atggagcttc
     5461 cgaacacctt tctcaggttg tcatctttca aactgtcaaa tcccaaagat attaataggt
     5521 ccaaggcttc cttatgtttt tcggttatgt tgaacaaagt atcttttata tctatactca
     5581 tagtatctcc ttttttcaat cttgtaagct aacaccttta tgtcatcctg agcgtaagcg
     5641 aaggatcaca tgagattctt cgagctaaag ctctcagaat gacacttcac aggttattca
     5701 tacatgattt attcataagt tattttaaca caatttaaac ttaattattg taacatttgt
     5761 tacaaacttt ccttcaccca tttaaataat tccatgggta tgtcaatatc tgatacctga
     5821 gtaattgttc taaatcctgc attgccgttc acttcgcata ctgtgaatga atcctttgca
     5881 aaaagcaaat ctatgccgca aaagtcaaga cctatggctt tggcggcttt aagagagatt
     5941 tctttgatag aatctgtcat ttcatatttt tcaactcttc cacccaatga atagtttgac
     6001 ttaaagccgc tgtcagaaat cctttttaca catcccaata ctttgtctcc cagcacatag
     6061 acgcgaatat ccattccgta gctggactca ataaattcct gacaaaaata ttcatccttt
     6121 attttatggt atacattatt ataatcttct tcgttttcaa tgagataaac tcccttgccc
     6181 tgggagcctt cgttttcttt catgacaaac ttcctcttgt caaagtaatt atgaatgaac
     6241 ttaaagtttt tttcttttgt gattaagaat ttaggagtac ttacattgtt tttaaccaaa
     6301 agctgagcag taactgcttt atttctggag ttaaacattg acagagtaga attaataacc
     6361 cttatgccta agttttcaag ctgcatgctt atagtgtaat tatagtctct cattattaca
     6421 aaatcaggaa gttttaagct cttaccgcta tatataaatt ctgttttgct attgttattt
     6481 aaaatgatta aatcatcgct aaaaacaata tcgatagata taccgttttt taaagcactt
     6541 tcttccatcc aatcaaatgc attgtttcca aatttattat ttatagtatt cttgggataa
     6601 atcatccatc cgtacatatt tacctcctgt atacatgaat tggtgccatg aattggtgcc
     6661 aggcaccatt tggcattaac tcaactcagc tgttcctgca ttcaatgcat agtatcgtcc
     6721 tttttgtgcc atcagttcat catggtcgcc gcgctcaata atttctccat gttctattac
     6781 cattatggca ttggcattgc gaaccgttga caatctgtgg gctataacaa atgttgttct
     6841 tcctgccatt aatttatcca ttccttcagc tattaatttt tcagttctag tatctacgga
     6901 agatgttgct tcatcgagaa taagaattat cgggtcggct attgcagccc ttgcaattga
     6961 caaaagctgc ctttctccct gagataaatt ttgtccgtct gaacttagta ccgtatcgta
     7021 accgtcagga agatgcttaa taaaataatg tgcatttgca agttttgctg cttctactac
     7081 ctcatcatcc gtagcatcca atcttccata cctgatattt tccttcactg ttccttcaaa
     7141 cagatgaaca tcctgtaata ctatgctcat ggtgcttcgc aaatcatgct tgttaatcct
     7201 ttttatatct attccgtcat agagtatttc tccctcattt atttcataaa acctgtttat
     7261 aagatttgta acggttgttt tgccggctcc ggttgagcct acaaaagcta tcttttgtcc
     7321 gggctttgca tagagaatta ttttgtttaa aactcttttt tcaggcgaat atccaaagtt
     7381 cacatcctta aattcaataa aacctttgag aggtactttc tcataatctc cgtcaccctt
     7441 tggaaccatc cagcaaagag ctttctttcc tgtgcaatct ttaagcaagg ttacgtctcc
     7501 ctcatcagtt tcgatttttt catccaatac gttgaatatt ctttctgctc ctgccaaggc
     7561 agcaaacaat gagttaagtt gatttgaaac cattgttatg ggtcttgata ctgttctggt
     7621 atatagaagg aatgatgcta tattgccgat actcagttgg ttaatcatta ctaaatatgc
     7681 ccctaacatt gatattatag catacataac aaaggacaga ttacccataa taggcatcag
     7741 cataacaccg tatgtagagg ccaaggtgct tgcattcctt aattcttcat tctttttact
     7801 gaacgtgtcg atagctctat cctcatagtt aaatacttta actacctttt gacctgacat
     7861 catttcttca gtataaccgt tcatatctcc caatgcggct tgctgcatgc gataattttt
     7921 tgcagatctt ttgcttacaa attttattga atagagcatt acaacaagca ttgcggcaac
     7981 cacggctgtc aaaatcggac ttaaaataat catcaccgta aatgttccga caactgttac
     8041 aaatgatata agaatttgtg aagcgctttg ctcaagtgat tggttaagca tatccacatc
     8101 attggtaaag gtggacatta attccccatg agagtgtttg tcaaaatatg aaacaggaag
     8161 tttttccata tgagaaaaca tgtcttcacg tattttataa actgtttttt gtgccaattt
     8221 cgccattgac aggttaccta tatattgaga aattgagcta attactacca atgctaccat
     8281 aataagaaga tactttacaa agagtccgct gtcattgtct ccgatcaagg catcaataac
     8341 gggactaatc attccgttta tccctatttc agccgaggaa ccgactatta taaagaaaat
     8401 acctccaaaa aacaagacaa gattatattt aaaataagaa aacaatctta gaaatgtttt
     8461 tttaggatct ttaggtttta actgtttttc tttcatactc ccaccccttt ttgctgagat
     8521 tcataaattt ctctgtatac aggagaagaa accagcaatt cttcatgttt ccctatagat
     8581 tctatttctc cttcattcat taccaaaatc ctgtctgcat gttgaatcga agaaactctt
     8641 tgagcaatta ttatagtggt tacatcattt aattcttcct tgaaaacacg ctgaattttt
     8701 gcatctgtcg ccatatctac agcactggtt gaatcgtcta atataataat tttaggagat
     8761 ttgagaagag ctcgagctat tgtaagcctt tgtttctgac ctccggaaaa attatctcca
     8821 ccctgctcta ccttgtggtc taatccgtcc tcataggtgg atacaaattc ccaagcttga
     8881 gctaacttaa gtgctctaat aatatcttca tcatttgcat tttcattgcc ccatttcata
     8941 ttgtcgcgga tggttcctga aaataatgtg ttattctgca gcacaaaggc tacattatca
     9001 cgcaatgttt ttatactgta atgctttaca tctacattgc ccacaattac gctgccttcc
     9061 gttgcatcgt aaagtcttgt aataagctgg accaaggttg attttgatga gcctgtggat
     9121 ccgataatcc ccaacacttc acctgaattt atatgtaaat tgatattctt taaagttttt
     9181 tccgtacttc cgggatagca aaaacatacg tttttgaagg tcacagagcc gtctgccaat
     9241 tcctttacag gattttttac ttctttaatt tctgactccg tgtcgattat ctctgcaatc
     9301 cttgttacgg aagcagaggc tctgagaagc tgcatgaaga aaaatgacat catcataaag
     9361 gacatcaaaa tttgtgttat atatgtaatg aaagatatta attctccgct tcccattgag
     9421 cctgcaacaa cttgttttcc tccaaaccac aacacggcta ttatggtaga ataaataatt
     9481 atatttaata caggcattaa aaacacaata agcgttatcg cttttaatgc agtaagcatc
     9541 aatgagtcat ttctttcttt aaatcttttt tcttcatgac tctgcctgtt gaatgacttt
     9601 acaacccgaa tagcagttaa attttcacgt ataattgcat ttaccctgtc aactcgtgtc
     9661 tgaaacttca aaaaaagcgg tcttgctttt gataaaataa taatcaatgc aataaccaag
     9721 acaggtatgg aaaccgcaaa tacctttgtc aaagatgcat taattcttat tgtcatcaaa
     9781 agtgcaaata tcatcataaa cggagctctt acagccatcc tcaagctcat catggttacc
     9841 tgccccaatg tgttgcaatc atttgtaagc cttgttataa gcgatgcaac cgtaaatttg
     9901 tccaaattcg caaaagaaaa gccctgtatc ttcttgtata gctttagcct cacttccgat
     9961 gcaaaaccaa aacctgccct tgcaccgtaa tgtgagctta ttacaccaga aatcatggct
    10021 aaaagagctg ccgcaatcat tataagtccg attttaataa catattcaat gtcacgattt
    10081 gcaataccta catccactat tcttgacatc agatatggta ttgctatatc ggcaataacc
    10141 tccaatatca tgagtatggg gcttagtacc gcaaatttta tatatttttt cataaatggc
    10201 aataactttt tcaatctgtt ctcctcttta cataatttat attatgttaa cnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttcaatga gtactcccac catctcagcg
    10381 aatttatcca acttcaatat acgagtgaat ttttgcccgt atattttttt gaccgatgga
    10441 atatcatcat ctcttcccgt aaaaatacag agaattgaaa ctccctgttt ccggcccttt
    10501 ctaacctcat ttgcagtatc gcttacgcct gtttcatccg aatattcttt ctgcagcgga
    10561 ataatacctt tcgaagtcac gcccaacata tcatttggtc ttccgtctga aagaacaatt
    10621 agtattctat ggctgtattt tgaatctttc atcatatgaa gagcagttcg aagggccatt
    10681 ccgtcccggt taaagcccga tgcatagtag ttgaaaattt tatcattgtt gtttttttca
    10741 tcataatcgc ggaaaagatt cataacagtg aaatccctca tgttgcagaa agaatatatc
    10801 cttacgggaa tattgcagcg cgtcagactt tctgcaatta tgtagccctc ttctgctata
    10861 acttcctgac gattaagctg ggatgctgaa gcatcaagca taatatcaac ggacatatca
    10921 ggtatttcat ctttaatact tctgacgaaa attttgtagt catcaagtac agtcctccaa
    10981 gctttttccg taacaagctt tcctgattca gtcttcattg aatatgtgta attaacaatc
    11041 attgcatttt taattctttg tgtaagtttt gttatgttgt ttgtgttcct gatataattc
    11101 tttatataat gttgtttatt tttatccctt tgctcagaag tttgcttctg tctgaaaatc
    11161 gcatcatcat tttcatcaaa ttgccccctt gtaaaatgga gatggcaact ttcatgagct
    11221 tcgtcgcaaa gtattttttc caactctttc gttttggatt cggcaattgc ggaaattccg
    11281 aaataatctt taatatattc cctctccttg ttatctgact tttctttgaa tttaaaccat
    11341 cctgaagcta ttttgccggt tccggttctc ccttcatcgg tatctataag catcaaggag
    11401 cttgaagggg attttcttcc gaagaaacgc agcaacatat tatcttttat tttttttata
    11461 taaaattcac tactatattc aggctcaaaa tatttattaa ttatttgctt caatttattt
    11521 attacttgct cggtattgaa atttatgtca aaatgtagct catttattaa gctctttaca
    11581 ttatctctga ctttcatatc atatccgaat acttggctga agtatgctgc tgaaatttca
    11641 tcaaacactt ctgcctcttc tcttttttca tacgcctttt tgccgtaatc aaaacgaaga
    11701 ccggctaata ccggtctgtt tttactatct ttttcaaaga tgcatccttc cagtccaagc
    11761 agcaatactt tctgataaaa aatatagtct ctgtcatacc tgagagtatc gaaaaattct
    11821 ctcaaaaggt tatagtcata atatttataa acagaaccaa ttatataatt ccaatataaa
    11881 tcagctttac ctttttcatc gtacgcagta atatcaggtg ttatggagta attctctgaa
    11941 acactccata ttatattcca tgctctttgt ttttcgcttg cttttctcat ttgatgatta
    12001 cttcctttat gcaatatact ttccgacatt tgacatactt aatttatgtc atcatcttat
    12061 tacgaagctt gttacccttc gtttgattta aatcccgata ccctgagaag ttttataccc
    12121 aagaatgtat ccaagtctat aactacatca tttttaggca gtaccaatgt cctagatata
    12181 tatacgtcta ttccgccaat attgataatt tgatagtttt caggtgcttt cggctttttg
    12241 gcaataattt ccggaatggg cacaagaacg ctttcgctgc ttaagcggtt ttccggaatt
    12301 tcaattgtaa tctgaagctt tccttttttc tttaggtgct tcattgccct ttcggttatt
    12361 tttattacat tttcatcaaa catttcatac tccttataaa agatgagatt cttcgggctg
    12421 aagccctcag aatgacacgt agcttattca tatcaataac ttttttagct tattaacatg
    12481 aaataaccca ctaccaatat acccaaaata attctatacc aaccaaatgc tttaaaatca
    12541 ttgtttttaa tatatcttaa caagaatctt attgctatta cagatacaat aaaggcaact
    12601 gtcataccgg tcagtaatac tgctaattcc aaacccgtga aatctattcc gaactttacc
    12661 aattttaatg cacttgctcc gaacattaca ggaattgata agaaaaatga atattcggct
    12721 gctatatttc tggatgtgcc aagtattata gctcctaata tggtagcccc ggagcgtgag
    12781 gttcccggta ccagggacaa tacctgaaag aagcctgtta ttaatgcggt tttataggat
    12841 aactctttaa aactctttat ttttgccggc ttacctttat ttttattttc aataattaaa
    12901 aacaaaatac cgtatacaat cagagttatg gctactgtct gataattata aaagttttca
    12961 tcaaaccaat catcgagcaa aaatcccaag atggctgcag gcagcacacc aactattacc
    13021 ttaaaccata tatccattgt atctcttttt tgtttcggtg tcttgcttgg agcaaatgga
    13081 ttcagtttat ggaagtatag caatacaacg gccataatgg ctcccaattg tataacaact
    13141 ctgaacattt ccataaattc atcggaagca ttaaatttta taaattcctc aaccaaaatc
    13201 atgtgaccgg tgctgctaat aggaagccat tcggttattc cctctactat tcccaatatt
    13261 attgctttga ttatttctat gtacattcgt cctccaaccc tcttaatttt ataacacata
    13321 tgaaattccg cagtctcgtt gcacaatttt gtttgctcct tccgtcgcac aaaattggca
    13381 ctcgttgcga atgacctacc gctatttttc tctcccttta gtcggaaaaa tagggttagg
    13441 gcatctttat tatatatttt gttaattaaa ttttcaataa aatataattt actcaaagtt
    13501 gctacggata acaaatacaa aacttgttcc ctcattaatc ttgctttctg caaaaagctc
    13561 tatatcgtgc cttaaagcta tttgttttgc aatagtaagt ccaagtcctg tacctgtttt
    13621 gttttcatct gattttacac tgtagtacct gtcgaatata tttggaaggt ctttttcgga
    13681 tatgccgatt ccttcatctc ttatagtaat ttgattgtct tttaaagtta tataaatgct
    13741 tttgttctca cgtgaaaatt taattgcatt atctataact gtcaaaaaca tttggcgaag
    13801 tcttccgtaa tctcccttgg ttaaaaacac atccttatcg gaattatatt gaatattaat
    13861 atttttttgt cttgccaagt ttgaagcact tcgtaaagaa tcctttaata cgtcacagat
    13921 attcagctct tgcatttcta ttttaaagtc gacattttgc agtcttgaca attccaataa
    13981 atcgtttatc aatctttcca agcttttgct ttcaataaga atttgtttgt aatattcatt
    14041 aatcttatcc gaatcggtta ttactccgtc ggaaagcgcc tcaagggagc cccttataac
    14101 agttacggga gttcttaatt cgtgagaaat atttgttata aaatcctgcc tttgtttttg
    14161 cactcttttt ctttcttctt cagctttacc gagcctttgg ctcaatatgt ccattgtttt
    14221 agcaaggtca cctatttcat cattttgttt aataccggtt tttacgctgt aattccctcc
    14281 tgccagcagg gctgccgtat ttttcatctt ttcaagcggt ttggtaaatg ttaaagctaa
    14341 ggcaacagat aaaagtacag ataaaattaa tgccataaaa atacttatta ataaaatcct
    14401 aaaaccttca cttgttgcat catttatacc ttgaataggc gaatgtatta aaagtgctcc
    14461 tattatttct ccatcagaag taatcggagt gcctatcgtc aaggcaggtg tttccaaaag
    14521 tttactgaac ccttcactga aagttgtttg gccttcaaaa acttttttaa caacaatatc
    14581 tgcatcttga ggcaggtcgg tataattgta ctgtatgtgg gacatgcttc ccgttattaa
    14641 aaggtttaat tcttcatcaa caatccatgc atctgccatg gctatatcat ccaaattccg
    14701 tatatataaa cctaagccgc cctgcattcc catcatgccg ccatgtctgc ccattccggg
    14761 tccgaaattt aaactgcttc catccattaa ttccgaaatt gtatctgcaa ttgtaagagc
    14821 ccgtcttttt agctcatttt tatgaatttc aactgcgtta tttttaaaca aaagcatgaa
    14881 tatattgcct ataattattg aaaaaacaat taatgcagct gaaaaataca ctatcagttt
    14941 tatagaaatt ttatttttca tcatatacct caaacttgta gcctaccccc catatagtct
    15001 taatctccca atttttatgt tggaaattat cgatttttga gcggagtctt ttaatatggg
    15061 aatccactgt ccggctgtcc ccgtaataat catatcccca cagactgttt aacaggtttt
    15121 ctcttgaaaa aactttgttc ttattaacag ccaaggtcca taatatttct atttcctttt
    15181 tcgttaggga aacattattt tcatcaatcg taacgatata atcttccata tttattttta
    15241 tattatcata tacaaataat ttactgctta tattttcagc ccctattctt ctcataacag
    15301 ctctcactct agccataact tcggcaggag aaaatggttt tacgatataa tcgtccgcac
    15361 ctatatcaag tcccattatt ctctcaaaat cctctcctct tgctgtaatc ataataagag
    15421 gaacatttga aactttcctt attctacggc atacttcaaa cccgtcaatt atcggcatca
    15481 taacatcaag caagactata tccggtttgt gtttatcaaa tgtatccaag gcttgcctgc
    15541 cattgtaagc aattttaaca tcgtagcctt ctttttttgc atattcctcc aatatggatg
    15601 ttatttgtct gttgtcatct gctattaaca ttttgggcat ataaaaatac ctcctatgtt
    15661 ttttgtaatt ataccatatg gtaataggaa aatgtgacaa ttatgtgttt ttataaaaag
    15721 aaaaatcata atttttttat actaatgaca taaatttgac acaaatattg tctatattaa
    15781 atctaaagaa aattcaggag gtaaaaaatg aaaaataaaa ttctaataat tggtttagta
    15841 ttattaataa tattggcaac atcaattact gtttttgcaa ttacgggaag aactcaaact
    15901 gcttatgatg aaagcgaaga tacttgggag aaattcagag atgaaatgtt tgaaaacaaa
    15961 aaggctttta tagaagaaag agttaaagac ggaactctta ctcaggaaga agccgatgag
    16021 attcttaaaa acattgaatt aatgcaggaa tactgccttg gagtaggcgg ctgcggaatg
    16081 ggaagaggct ttggaatgat gagaaatagg cttggtaaca atagcggata tggcaactac
    16141 ggcttcagag gtatgggctt tggcggaggc agatgcggca gaggttcttg gtaaaaccct
    16201 ttgctaaaac tcgggatgac ataaagtacg gttattgtca tattagaatc acattccttt
    16261 cattgaaaac atattatagt actaataaaa tacagaaagg aatttgaaaa aatgtacaat
    16321 tattataact atccgtgcca aaatatatac ccttggtata gacaacaaga taggatgtat
    16381 aatcgccaaa gaatgtataa tccatacaat aatctaatga accctgattt taacaattat
    16441 cccagatatc ccacttaccc ggactatcct tataatcagg aatcttttta cagtccctgg
    16501 tcattacaat atccgagcga cagaggcttc atagaaataa aggattacgg tccaaatccg
    16561 tttgtaatca atattgagga agcaacagaa caaaacaata ctttccgttc aactctatgg
    16621 acaggggatc atttgcaact cactttaatg agcttaaatc ccggagaaga tataggtttg
    16681 gagatgcacc ctgatgttga ccaatttata cgaattgaag aaggtcgggg aattgttcaa
    16741 atgggagaca gtgaagataa tctggatttt gaagaaatgg taagtgaaga ctttatattc
    16801 ataatacctg ccggcaagtg gcataattta accaatatag gaaggacacc ccttaaacta
    16861 tattcaattt atgcaccgcc cgagcaccct catggcacag tccatgaaac caaggctgaa
    16921 gcagaagctg tgcatggtaa ctaatatttt tcaataataa agcggtttct cccaaaggag
    16981 aaaccgcttt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttattaatc
    17101 taattgcaga accttgtaaa gtattaccgc catttcagct cttgaaatca ttgattttgg
    17161 atataagtat gaattgtttc cttgaattat gcctgccttt accaatgttg caacagaggt
    17221 aacagcataa tcggatactg aatttctatc cttaaaagca tataaatcac tttccttacc
    17281 tgcagataat cttttattct caattatcaa tgccctgtca acaagaacca ttgcatcctc
    17341 tctggttatt cctgcagtcg gcataaattt atccccgtaa ccctttgcta taccgagtga
    17401 tttggctact gcaattgctt tatagtagta gctgtcctta ggaacatcac tgaaattacc
    17461 tctgtcagtt gccgtcaagt ccaaagctct gtacaacata agcatgaaat ccccgcgggt
    17521 tacattttgt gccggactgt aattgttgtt tccggttccg gcgactacac cttctttaaa
    17581 caaataatca atctgagatg ctgcccatga ataatccttt gttacatcct tgaaatacaa
    17641 agaaccttca gtcttaggcg cggctacaac ttcaacctta accgaacctg taaatgaaaa
    17701 accgtctgta ttgtatccgg tatattttat ggtaaccgtt ccggaataat ctttataagg
    17761 tacaaatgtt accctgtata aagaaggagt atcttcatag taatatttct tgcttgaggt
    17821 cacctcggag ccataattac ttgatgacgt gtaattataa taaagtttgc cgtattttga
    17881 tgaaggcaga gtaaatgtta catagtccaa ttcaacccct gtggaatctt cggatgcatc
    17941 attgaaatct tcatcatcaa atcttatcgg tgtgttttct tttgttgaaa ttttaatatc
    18001 atcagcaacg ggaacttctt ttgttaccgt aattttaacg gcacctgtat aagatttgcc
    18061 atcaatgttg tatcctgtgt aagaaataac tacggtacct gtataagatg gtttcggaac
    18121 aaatgttata tcatccaaat catcatcata atacttggtg gttgaagaaa ctttggattg
    18181 acttcttgat gaataattgt aatacaaagt tccatatgtt gatgaaggta aggtgaatct
    18241 gacataatct aatcttttac ctgtaacatc acggcattcg tcatcaaaat catccgcatc
    18301 aaaatctatt ggtgtatatg agcctgtttt gtatgttatg tcatcagcat ctgtaacaac
    18361 ctttaatact tggatttcaa tttcacctgt ataggattct cccttactat tataacctgt
    18421 ataagaaatc actaccgttc cggaataatt tttatacggt acaaaggaaa tatcatctat
    18481 atcatcatca tagtattttg aagtcgagga aactttagat tgactgctta agccgtagtt
    18541 tgaatacaga gttccccttg atgaagatgg gagagtaaac ttaacataat ttaatctttg
    18601 cccggttcca tccagacaca cctcttcaaa atcatctgcg ttaaaatcca ctaatgtatt
    18661 tacatataaa gaatatttaa ttttatctat tacaaatact ttttctgcaa cggttatttc
    18721 tactttgccc gtaaacacat caccggctgt attataacct ttgtatggta ttgtgaattt
    18781 tccgaaatac ccggaattag gaacaaaaga cattttatct aaatccgctt tactgtattc
    18841 tttgccggct tcaacagctg ttcccttatt tgttgaagat acataatcaa tatagatttt
    18901 accgtttgaa ggcattgaat caaaatttat ataattaaag gtgccaccta ctgtt
//
LOCUS       DAOSHP010000032        18780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2394_length_18780_cov_9.453618, whole genome shotgun sequence.
ACCESSION   DAOSHP010000032 DAOSHP010000000
VERSION     DAOSHP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18780
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2394_length_18780_cov_9.453618"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..812)
                     /locus_tag="PLB33_04760"
     CDS             complement(93..812)
                     /locus_tag="PLB33_04760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOG62867.1"
                     /translation="MKLNLFVTAITPGIALALTFYWFDRHDREPISMLLKVFVLGALY
                     VIPAILIENFLLFFNIFGGILGAAYTAFIVAGLTEEYIKRKVVLKYVYFNPVFNEKLD
                     GIVYCVMSALGFATFENIMYVVFLFSDVESVGYYRAIISVPAHMLFAVTMGYYLSLAK
                     FSSTPEEHRYFFKKSLTVPAIMHGIFDFILMSNIQPLMFLFIPFVIYLWTANLKKLNI
                     YYNESKFYANNNNEFNYFDDE"
     gene            complement(855..3275)
                     /locus_tag="PLB33_04765"
     CDS             complement(855..3275)
                     /locus_tag="PLB33_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG62868.1"
                     /translation="MIIKKYTTGRFAGIKDVKIEFKDGLNVILGSNESGKSTLVEGIH
                     SVLFKPSRIGRKTAEDKEFHSKFMPYPIGDSIDGEIDLEHNGEIYTLRKEWGADASSK
                     LTMPNKIVIKSDISINDSLKDVYIYGEGTYSSVFFLKQYYLKEAINRIVENKEATGEI
                     SSLLRSTIMELDGVSLESLGQKIQDEIDTLLKRWDIERNYPENNKGINNPYKVGFGKV
                     VESYYAKERIKLEMAKAEEAEKKFNEICVQIKKTEEKIEELKAKKESMEELENDVIQR
                     SILEPQIEQLNKDISALSKINRDWPRSEEKLKQLITELTRLNEKYKKLEEEKELSKKA
                     EEKNILEKNLANVDEINARIKELKAQISGIKTVTKEDIKALETNYNGMNKAEAMMKAG
                     VIMAKLNYFQDGKKLTVTKDLEEPTEVNAGDSFTAKGFMKLQSENLIDIEIKSGNIDF
                     NEIRIQYENFKKNYEQLLKTLEVKNLEEAKLNKEKLDSLNRTLESCIMQKNQYLGELT
                     YEELKDRIRELGDLSLVRNTAIVESEMTSINAEKIELISEKKLLESNIDKWAEEYKNL
                     DGLFNKIIEIKLKQNGIKTNLDKLAELPPEYKSTDEFRRILTEIRNKYGYLINTLASL
                     KEEYYRLEKELPESTYEELSESLKQAERLFEKRLEKGKRLLKIKENFENIKLKMDEQS
                     FIPVITAFSNYLNLLTNGSYKATDIDDDFNLKLKNENEVEMPLSLLSSGTYDCVALAL
                     RLAIAEYILGDNKGFLILDDCLVDLDPNRKDTAVKLISRFANKHQVIFTTCSPDTATL
                     LGGYLIKI"
     gene            complement(3265..4380)
                     /locus_tag="PLB33_04770"
     CDS             complement(3265..4380)
                     /locus_tag="PLB33_04770"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG62869.1"
                     /translation="MIKIFHTADVHIGMKFNNYPEPVKGYLKEARLDVLDNMIKFANE
                     EKCNLFVIAGDLFDNITGIDKKTKDRVITALNNFQGECVLVIPGNHDYDNGMIDLWDS
                     FNKAASDKIVIINEECAYSLEDYGLNAVIFPAPCHSKHSSTNNLQWIKEKEIDDNYVN
                     IGIAHGALEGLSPDLDKNYYNMSIKELNNIPMDLWLLGHTHITYPFKSPITNEKIFNP
                     GTPEPDGLDCKHNGQAWIIIVDDNKKTNAKLIQTGTYKFKDKVYTIEDRDDLDKILEE
                     LIKDEPEKTIARITLEGSVDEDVFQYRQEIYGSIEKNIIYLIVEDSDFKMKITTEKIH
                     REFSDGSFPQELLLSLSDDEDTLQLAYELIMEVRNDN"
     gene            complement(4634..4831)
                     /locus_tag="PLB33_04775"
     CDS             complement(4634..4831)
                     /locus_tag="PLB33_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62870.1"
                     /translation="MYKYDYNYNKDMNYNLKVFCQSEKVNEEYMRVLLFEEKSKFLIN
                     KIAALKAMALVPIKWFLTLIK"
     gene            complement(4949..5389)
                     /locus_tag="PLB33_04780"
     CDS             complement(4949..5389)
                     /locus_tag="PLB33_04780"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOG62871.1"
                     /translation="MELEQKIKMLQVIYAGALADSVLRMGKEGILEKVALEKREEQMH
                     TGKYRASQFGIDKPEDVFLVLSELFGCANWSVNSDNEGLKAEATKCMLCALAKKIGAQ
                     SPCNIFCLDPMEGMIRGLNPDINFEVTETLWSGQKCKVNVLNKK"
     gene            complement(5480..6169)
                     /locus_tag="PLB33_04785"
     CDS             complement(5480..6169)
                     /locus_tag="PLB33_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG62872.1"
                     /translation="MESVINNNLTIEYIIENYGKMVYSICRRMIQNKDTADDAAQEVW
                     LEVNKSLKTFRGESKISTWIYTITSRIVLKMAKEEHKYSTYFLSNYFHGEDIEIPDYE
                     DFDKQLWVREMCDKCLTGTLHCLDNESRLAYILRDINMLPYSDIAAIFEKDEASIRKM
                     VSRSRNKLRNFLNDECILKNPDSKCKCRMSKWVKEIQLDEEYKKLRQTVNRINFYRAS
                     EIILTKDYLLK"
     gene            6526..7494
                     /gene="msrB"
                     /locus_tag="PLB33_04790"
     CDS             6526..7494
                     /gene="msrB"
                     /locus_tag="PLB33_04790"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOG62873.1"
                     /translation="MDKELKGNKLIYLAGGCFWGTEKYLGLINGVVKTEVGYANGNTE
                     NPSYEDVCRRNTGHAETVKVVYNPKVIDLETLLKLFYKAIDPVAINRQGYDIGTQYRT
                     GVYYVDKEDEAVIKESLNELQKEYKEPLAVEVTELKNYYKAEEYHQKYLEKNPGGYCH
                     IGELEFYELEKAQKNSKYKVKPKKELESTLTEMQYNVTQNNATEPPFDNEYYDNFEDG
                     IYVDITSGEPLFTSKDKFDSGCGWPSFSKPIDEKVVKEKSDLSYGMIRTEVRTSNSDS
                     HLGHVFNDGPKESGGLRYCINSAALEFIPVDKMEERGYGDLLYLFE"
     gene            7706..8224
                     /locus_tag="PLB33_04795"
     CDS             7706..8224
                     /locus_tag="PLB33_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOG62874.1"
                     /translation="MTKTKEVILCGLLLATGIILPMFFHMFGLTGPIALPMHIPVLIG
                     GFLLSPKLALLLGIITPVLSGMLTGMPVMFPMAVIMAFELGIYGLAASLAVRKFNLSV
                     IPSLIVSMIAGRIAAGLTVAILVELFGVKMNPLIYIKGAIITGIPGIIIQLIFIPALV
                     YAIKSYVKIKSV"
     gene            complement(8221..8589)
                     /locus_tag="PLB33_04800"
     CDS             complement(8221..8589)
                     /locus_tag="PLB33_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOG62875.1"
                     /translation="MYNIKIKRVYQDSSEDDGYRILVDRLWPRGISKEKINIHKWAKE
                     ITPSTELRKTFKHDPDTMDEFRVRYNFELDNNEHALDFINLLKDKLKEGNVTLLYAAK
                     NEEFNHAIVLKEWIERMISN"
     gene            complement(8639..9577)
                     /locus_tag="PLB33_04805"
     CDS             complement(8639..9577)
                     /locus_tag="PLB33_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG62876.1"
                     /translation="MNKIIQFKKKIFTRDNIGYLMIFAAGCLWGTIGLFVNLLNSAGA
                     DTTLTAFMRMFMGCLILIPIMLHKGGVSLFKIDKKGLSQCFMLGILSQALFNYSYITS
                     IRSVGIATAAILLYTAPVFVAIMSRMLFKESIGRIKILALIINIVGCFLMVTGGNLSN
                     IKLSAAGIFFGVAAGFLYSLVTIIGKSTSGDIHPFTVVFYGFLFGGIAIGIFTSPWES
                     IKTVSGLKFWIYSFGYGLIPTVGSYLLYLGGLRKQIEISKTPVIASVEIVVATLIGII
                     VFEEGLNFINMLGIIIIFSSIVIINMKTPYEGARHQ"
     gene            complement(9574..10233)
                     /locus_tag="PLB33_04810"
     CDS             complement(9574..10233)
                     /locus_tag="PLB33_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62877.1"
                     /translation="MARRKRVYSRRRRINYGRVAFLIAVLVVIILLVMKIFSKNRYIS
                     KVEEALEKDITRIAGSISTVSKIDATIYENEGIKYTNQHENIKRIMTFDGSLSEDMDK
                     AGDVKILLENLLISEKTEVTAELPPKDDGYYWIEADIFTKDKVLIFSVEEEYNFDLYY
                     DIENKTVYVKEKYYDEFSKKYNKVKFQGYSVTDEFRNIINSMANTGLDTSGTQIPETD
                     Q"
     gene            complement(10235..10852)
                     /gene="coaE"
                     /locus_tag="PLB33_04815"
     CDS             complement(10235..10852)
                     /gene="coaE"
                     /locus_tag="PLB33_04815"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOG62878.1"
                     /translation="MKQNRPKVIVITGSIGTGKSTAVDIIKEFGFTVLDSDKIVHEGY
                     NIGSELYYKIINHFGEDILKGDEIDRQKLGEIVFNNDKKLNELNNMVHGYVYDKLKEG
                     IEASKEKVIFLDIPLLFETKDIHEPIYDEIWLIYVSEETQRKRLMERAIAENKKPEDV
                     LKIINKQISIKEKVFMADVVINNEGTVEELKENIKALLEIRGMGW"
     gene            complement(10839..13538)
                     /gene="polA"
                     /locus_tag="PLB33_04820"
     CDS             complement(10839..13538)
                     /gene="polA"
                     /locus_tag="PLB33_04820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOG62879.1"
                     /translation="MKKIMILDGNSLLFRAFYALPPLKTKKGQYTNAVYGFLSMLYKI
                     IDTYSPEYLCVAFDPEKPTFRHEKYKDYKANRQKAPNELVQQFKLIRDVLDVHNIKRV
                     EVEGFEADDVSGTFACAAKEQGADVYLVTSDKDYLQLIDENVRVILTKKGITNIVEMD
                     INSMGEEYGLKPEQFVDLKALMGDSSDNIPGVSGVGEKTALKLIQEYKSLDNLYENIN
                     KITGKLKEKLETDKMQAYMSQTLARIITDIPIEFNIEEFKIKKPDIKKLSELYDELEF
                     RQFKKRLVETIPVDDNGQISLFDTDRAETVKNIDIIYIDSEEKLTEIIKNISRKEKAI
                     LKFLLDGERALFSNALALGISDGENIYYIDFEKAEEKKVLNELKEIFESEKIAIWGHN
                     LKNEIIYLMKNNIQINNIQFDSEIGKYLLNPSESSYSLDKIAYEFLNVEIPSENQILG
                     TGVKRKTFKEIDIEQRKAYIFNYLNTIGKVQKQITEDIDELKMTCLYEEIELPLIEVL
                     AYMEFVGFKVDISVIDKLGVHFQEKIAELEKDIYNIAGESFNINSPKQLSVILFDKLG
                     LPIIKKTKTGISTDIEVLERLSSEHAIVDKIIEYRQMVKLNSTYVEGLKNEVNKKTGR
                     VHSIFNQTIASTGRISSTEPNLQNIPTRTEEGRELRKAFVPEDDYILCDADYSQIELR
                     VLSHLANEQNLIDAFEQGLDIHAKTASQVFHVKLSEVTSEMRGRAKAVNFGIVYGISD
                     YGLARDLKIPRKEAKKYIDNYMKYYSNIEKYMKDIVEQGKRDGYVTTYFGRRRYIPEL
                     TSRNFNIRSFGERIALNTPVQGTAADIIKAAMVGVYRRLKNNKMKSRLILQVHDELII
                     EAYKDELEDVKTILSEEMENVVVDFKVRLKSDINTGSSWYEAK"
     gene            13680..14267
                     /locus_tag="PLB33_04825"
     CDS             13680..14267
                     /locus_tag="PLB33_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOG62880.1"
                     /translation="MKLEPKNYNFSNVRYFSPIQLSEHYQLYINYISCLNKVNNDLKN
                     DLIYDECNSNYSDIRSAQKSKTFCFDSIKLHELYFENLTGNNTRIHGPIERVLVNTFG
                     SYNNFREKFKCIGMSMRGWVLLCYDRYNNEFYIYGQDSHDCGVMLYAEPLIVMDVYEH
                     AYMIDFGTNRNVYIDAFFQNIDFNVINQRLNKILK"
     gene            complement(14504..15121)
                     /locus_tag="PLB33_04830"
     CDS             complement(14504..15121)
                     /locus_tag="PLB33_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOG62881.1"
                     /translation="MKNLTINEFAKIVASDSPVPGGGSIAALCGALSAALSEMVANLT
                     VTKKKYADSKEEMNAIINKASQLRDRLLNYIEEDSLAYKKVMEAYKLPKETEEEKCLR
                     LERIQEGLKVAASVPLEVAETSYEIFPLVEAVVLRGNSSSVTDALVGAMMARTGVLSA
                     ILNIRINLDSIKDEDFVKVLKEKADILEGKTRQYEKKILELAPFK"
     gene            complement(15118..16365)
                     /gene="hutI"
                     /locus_tag="PLB33_04835"
     CDS             complement(15118..16365)
                     /gene="hutI"
                     /locus_tag="PLB33_04835"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOG62882.1"
                     /translation="MSKLIIKNASELVTCKGKAPKHGKDMSDIGLIENGCLVIEDDII
                     VDVGTSNILKNYDEDDYQIIDAKDKAVLPGFIDSHTHLIFGGYRADEFSWRLKGDSYM
                     SIMERGGGITSSVRATRNTSLEELTALGKTRLDKILAMGVTTVEGKSGYGLDCETEIR
                     QLKAMKQLNDMHPVDIVPTFLGPHSVLPEYKGKEDEFIQFMIDDVLPKVKEENLAEFA
                     DIFCEKNVFTIEQSRKFLTAAKNSGLKLKIHADEMYQLGGAELAVELGCTSADHLLQA
                     SDEGIRQMSKSDTVATLLPGTAFCLKEEYARARYMIDSGCAVSLATDFNPGSCFTNSI
                     PLIIALAALHMNMTIEEIITSLTINAAAALGRSDTRGSIEKGKKADIIILEYPSIHFL
                     PYHVAVNIVETVIKNGKIVRGLL"
     gene            complement(16440..17330)
                     /gene="ftcD"
                     /locus_tag="PLB33_04840"
     CDS             complement(16440..17330)
                     /gene="ftcD"
                     /locus_tag="PLB33_04840"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOG62883.1"
                     /translation="MDRIIECVPNFSEGRNTEKIEKILDVFRGKKGVKLLDYSSDENH
                     NRTVVTVVGEPEEIKKVMVEAIAKAVELIDLNYHQGQHPRMGAVDVVPFIPVRNVTVE
                     EADKLAKDVAKEAADKYNLPFFLYEKSASAPHRENLANVRKGQFEGMKEKMKDELWKP
                     DFGPDTIHPTAGVTAIGARMPLIAFNVNLSTPDIEVANRIAKQVRHISGGFRYVKAIG
                     VEIHDRGLVQVSMNLTDYTRTSIYRVFETIKMEAARYGVNVVGSEIVGLIPMQALVDT
                     AEYYLRLENFKYDQVLETRL"
     gene            complement(17323..>18780)
                     /locus_tag="PLB33_04845"
     CDS             complement(17323..>18780)
                     /locus_tag="PLB33_04845"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG62884.1"
                     /translation="KATAMGCSSYGQMTAGGWMYIGPQGIVHGTFNTLINAGRKELGI
                     TDDKDLAGHLFITSGLGGMSGAQAKAVEIANGVGIIAEVDYSRIETRLKQGWVSTVTD
                     NLEEAFEIAREYLYKKQTISIAYHGNIVDLLEYAADNNIHVDLLSDQTSCHVPYDGGY
                     CPKGLTFEERTELLNSNREKFVQMVNKSLADHFKAIKTLVDRGSYFFDYGNAFMKAVF
                     DAGVKEIAKNGNDTSEGFIFPSYVEDIMGPMLFDYGYGPFRWVCLSGKPEDLHKTDQA
                     AMSVIDPNRRGQDRDNYIWIRDAEKNKLVVGTQARILYQDALGRRDIALKFNDMVRKG
                     EIGPVMLGRDHHDTGGTDSPFRETSNIYDGSNVTADMAVHCFAGNAARGMSLVALHNG
                     GGVGVSKAINGGFGLVLDGSKRVDEIIKTSIPWDTMVGVSRRSWARNEHSIETTIEYN
                     KKFEGEDHITIPFLAKDELIEETLKNYKNKGERNG"
BASE COUNT         5349 a         3754 c         2907 g         6770 t
ORIGIN      
        1 tgtaatttga ttttataaaa tcagccataa attctccttg tatattatat tgtcccgcaa
       61 aattctttca ctagaaaaag atgcagcata ttctattcat catcgaaata attaaactca
      121 ttattattat ttgcatagaa ttttgattca ttataataaa tatttaattt ctttaaattt
      181 gcagtccaca aatagattac aaacggtata aacaaaaaca tcagcggttg aatgtttgac
      241 attaatataa aatcgaaaat tccgtgcata atagccggta ccgttaatga ttttttgaag
      301 aagtaacggt gttcttccgg tgttgaggaa aacttagcca gtgaaagata ataccccatt
      361 gttacagcaa acaacatgtg tgccggaacc gaaataattg ctctgtaata acccactgat
      421 tcaacgtccg agaacaggaa tacaacatac attatatttt caaaagttgc aaaacccagg
      481 gctgacatta cgcagtatac tattccatcc agcttttcat taaaaacagg attaaaatac
      541 acatacttca gcacaacttt tctttttatg tattcttccg ttaaaccggc aacaataaat
      601 gccgtgtagg cagctcccaa aatacctcca aatatattga agaataataa gaagttctca
      661 ataagtattg ccggaataac ataaagagct ccaagaacaa acacctttaa taacatgctt
      721 ataggctctc tatcgtgtct gtcaaaccaa taaaaagtca atgccaatgc tatgcccgga
      781 gttattgctg taacaaataa atttaatttc acaacaagat ttacctccac tattcttcat
      841 taaatccaac caatctatat ctttatcaaa tatcctccaa gcaaagttgc tgtgtccgga
      901 ctgcaggttg taaatattac ttgatgttta tttgcaaacc ggctgatgag tttcacggct
      961 gtgtcttttc tgtttgggtc taagtctacc aaacaatcat caagtattaa aaatcccttg
     1021 ttgtctccca atatatactc tgcaatggca agtcttaagg ctaatgcaac acaatcgtat
     1081 gttcctgaag acaaaagact aaggggcatt tctacctcgt tttcattttt tagctttaaa
     1141 ttaaaatcat cgtctatatc tgtggcttta tagctgccgt ttgtaagcaa attcaagtaa
     1201 ttggaaaaag cagtaattac cggaataaat gattgctcat ccatttttag cttgatgttt
     1261 tcaaagtttt ccttaatctt taacagtctt ttcccttttt ccagtctttt ttcaaacagt
     1321 ctttctgctt gtttcagcga ctcagacagt tcttcatatg ttgattccgg taattctttt
     1381 tcaagccggt aatattcttc ttttagactt gccaaagtat ttattaaata accgtattta
     1441 ttcctgattt cagttaaaat tcttctgaat tcatctgtgg atttatattc gggtggtagc
     1501 tctgccaact tatccaaatt tgttttaatt ccgttttgtt ttaattttat ctctatgatt
     1561 ttattaaaca aaccgtctaa atttttatat tcttctgccc atttgtcaat attgctctcc
     1621 agcaatttct tttctgaaat cagctctatt ttttcggcat ttatggaagt catttcggac
     1681 tcaacaatag cagtgtttcg aacaagcgac aagtctccca attctcttat cctgtctttt
     1741 aattcttcat aggttaattc tccaagatat tggtttttct gcataatgca tgattccaaa
     1801 gtcctgttta agctgtccaa tttttccttg ttgagttttg cttcttcaag attttttact
     1861 tccagtgttt ttaaaagctg ctcgtaattt ttcttaaaat tctcatattg aattcttatt
     1921 tcattaaaat cgatgtttcc tgatttaatt tctatatcaa taaggttttc ggactgaagc
     1981 ttcataaagc ccttggcagt gaagctgtct cctgcattaa cttctgtcgg ctcttctaaa
     2041 tccttggtta ctgtaagctt tttcccatct tgaaaataat taagcttagc cataattact
     2101 cctgccttca tcatggcttc tgctttattc atgccgttgt aatttgtttc aagggctttt
     2161 atatcttctt ttgtaaccgt ttttattccg gatatctgtg ccttgagctc ttttattcta
     2221 gcatttatct catcaacatt agctaaattc ttttcaagta tatttttttc ttccgctttt
     2281 tttgataatt ccttttcttc ttcaagtttt ttatattttt catttagcct tgtcagttct
     2341 gttataagct gtttcaattt ttcttcgctt cgaggccaat cccggtttat ctttgaaagg
     2401 gcggatatat ctttattaag ctgctcaatt tgaggctcaa ggatagacct ttggattaca
     2461 tcgttttcta attcctccat ggattctttc ttggccttta attcttcgat tttttcttcg
     2521 gtctttttaa tctgtacgca gatttcgttg aatttctttt cagcctcttc tgccttagcc
     2581 atttccagtt ttattctttc tttggcatag tagctctcca ccactttacc aaaacccact
     2641 ttgtacggat tgtttatgcc cttgttattt tcaggatagt tcctttcaat gtcccatctc
     2701 tttaataagg tgtcaatttc atcttgaatt ttttgtccca agctttcaag agatactccg
     2761 tcaagctcca taattgtgct tcttaacaat gagcttattt cacctgttgc ttctttattc
     2821 tcaactatcc tgttaattgc ttccttaaga tagtattgct ttaaaaagaa cacgctgcta
     2881 tatgtccctt caccgtaaat gtatacgtcc tttaaggaat cattgatact tatgtcgctt
     2941 tttattacaa ttttattggg catagtcaat tttgaagaag cgtctgctcc ccattctttc
     3001 cttaatgtat aaatttctcc attatgctct aagtctattt ctccgtctat gctgtcccct
     3061 atgggataag gcataaattt agagtgaaac tctttatcct ctgctgtttt cctgccaatt
     3121 ctgctcggct tgaaaagcac tgaatgaatt ccttcaacca aagtggattt tcctgactcg
     3181 tttgagccaa gaattacatt gagcccgtct ttaaactcga tttttacatc cttaatgcct
     3241 gcaaatcttc cggtggtata ttttttaatt atcatttctt acctccataa taagctcata
     3301 agcaagctgc aaggtgtctt cgtcatctga aagtgacaaa aggagttcct gaggaaatga
     3361 accgtctgaa aattctctgt gtattttttc agttgttatc ttcattttaa aatccgaatc
     3421 ctcgactatt aagtatatta tattcttttc aatcgagccg taaatttcct gcctatactg
     3481 gaacacgtct tcatcaacac tgccttctaa ggttattcgc gctatggttt tttccggttc
     3541 atcctttata agctcttcaa gtattttatc taagtcatct ctgtcttcta tcgtgtaaac
     3601 tttatcttta aatttatatg tgcctgtttg tattaacttg gcatttgttt ttttattgtc
     3661 atcaacaata ataatccaag cttgtccatt gtgtttgcag tccaatccgt caggctcggg
     3721 ggttcccgga ttaaatattt tttcattggt aatcggtgat ttaaacgggt atgtaatatg
     3781 tgtgtgccct aatagccaca ggtccatagg aatattatta agctctttaa tcgacatatt
     3841 gtagtagttc ttatccaaat ccggggacaa accttcaaga gccccgtgtg cgatgcctat
     3901 atttacataa ttatcatcaa tttctttttc tttaatccac tgaaggttat tggtgcttga
     3961 atgctttgaa tggcatggag caggaaatat aacggcatta agaccgtaat cttctaaaga
     4021 ataggcgcac tcttcgttta taataacaat cttatcagaa gcagctttat taaagctatc
     4081 ccacaggtca atcataccgt tatcgtaatc atgattgccg ggtataacca acacacattc
     4141 tccttgaaaa ttatttaatg cagttataac tcggtccttg gtttttttat ctattccggt
     4201 tatgttatca aacaaatctc ctgcaatgac aaacaggttg catttttcct cgtttgcaaa
     4261 ttttatcatg ttatctaaga cgtctaatct tgcttcctta aggtagcctt ttacaggttc
     4321 aggataatta ttgaacttca ttccgatatg tacatcagct gtatgaaaaa ttttaatcat
     4381 tttttcacct cattaatatt tcggggatgt tttggggatg cttcttttgt tctcatagtg
     4441 acaaagtacg aggtatgttc tatgttgtta atattatata gcagaatact gcaattatca
     4501 acaagcagaa aataaaaagt ccttcctgaa atattggtgc cttattggtg cctggcacca
     4561 aatacatggc actaaaaagt aggtgccaag cacctggttg gctttgatgc ttggcaccta
     4621 cattcttatt cacctatttt ataagtgtaa ggaaccattt gattggaaca agagccattg
     4681 cctttaatgc ggcgatttta ttaattagga atttactttt ttcttcaaaa agcaataccc
     4741 tcatgtactc ttcattaact ttctcacttt gacaaaaaac ttttaagttg taattcatgt
     4801 ctttattata attatagtca tacttataca tatcagtcct cactactgcc aatacatttc
     4861 caacaattca tttttcatat gtgcctttct cacaataaag ttaatttcaa tagagcaaac
     4921 taaccttagg taaattatgg aggctaccct attttttatt aagcacatta actttgcatt
     4981 tctgacctga ccataaagtt tctgttactt caaagttaat atccggattt aagcctctaa
     5041 tcataccttc catcgggtct aaacagaaga tattgcatgg actttgagct ccaatttttt
     5101 ttgccaatgc acatagcatg cactttgttg cttcagcctt taagccttca ttatcgctgt
     5161 taaccgacca atttgcacaa ccgaataatt ctgatagcac taaaaataca tcttccggtt
     5221 tatcaattcc aaattgcgat gcacgatatt ttccggtgtg catttgttct tctctctttt
     5281 ctaaggctac cttttcaagt attccttctt tccccatcct caaaacagag tctgccaatg
     5341 ctcctgcata aattacctga agcattttaa ttttttgttc taattccatt ttaaacctcc
     5401 aaatttttta ataaaaaaac cttccatatt tgttttcaat acattagtgg aaggtttttt
     5461 gtttttgtga caactaaatt tattttaata aataatcttt cgtaagtatt atttcagaag
     5521 ccctgtaaaa atttattcta tttactgtct gccttaattt tttatattcc tcatctagtt
     5581 gtatttcctt aacccattta ctcattctgc atttgcactt tgaatcagga ttttttaata
     5641 tgcactcatc atttaaaaag ttacgcaatt tattacgtga acgagatacc atttttctta
     5701 tgctggcttc atctttttca aaaattgctg caatatcgga ataaggtaac atattaatat
     5761 cgcgtaaaat atatgctaat cttgattcat tatccaagca atgcaaagtt cccgtcaggc
     5821 atttatcaca catctctctt acccataact gtttgtcaaa atcctcataa tcgggtattt
     5881 ctatatcctc cccgtgaaaa tagttgctga gaaaatatgt agaatattta tgttcttcct
     5941 ttgccatttt caaaacaatc cttgaagtaa tcgtatatat ccaagtggaa attttggatt
     6001 cgccacgaaa tgtcttaaga cttttattaa cctccaacca aacctcttgg gcggcatcgt
     6061 ctgcagtatc tttgttttgt atcatccttc tgcatattga atagaccatc tttccataat
     6121 tttcgatgat gtattcaatt gtcaaattat tatttataac actttccata catacctctc
     6181 gtatttatat aaaaactctt ttatgcaatc tttcccatat tataacacat taaaaaagat
     6241 tttacattaa ataaaaaaat tctatcctgt cctcaaccat ccttaggtga cctcaaaaaa
     6301 ttgtattgct gataataaaa aatacctgtt ataatgatta cgacaagggt atagagtaag
     6361 tataaattaa tcgattataa taatggaaag gttactatga ttaagaaatt gattttactg
     6421 ttttttttgt taatattggt aattgttttc tttgcttgtg ataataataa gaagagttac
     6481 aatatgacag caaataatga taatttaaga aaggatgatg aacttatgga taaagaatta
     6541 aagggcaata aattgattta ccttgcagga ggatgttttt ggggaacaga aaaatattta
     6601 ggcttaatca acggagtagt taagactgag gtaggatatg caaacggaaa tacagaaaat
     6661 ccttcttatg aagatgtctg caggagaaat accggtcatg cggaaactgt taaagtggtt
     6721 tataacccga aagtgattga tttagaaact ttacttaagt tattctacaa agcaattgac
     6781 ccggtagcta taaacagaca aggctatgat atagggactc aatatcgaac aggcgtgtat
     6841 tatgttgaca aagaggatga agctgtaatt aaagaatcat tgaatgaatt gcaaaaggaa
     6901 tataaggagc ccttggctgt tgaagtgacg gaacttaaga attattacaa agcagaagaa
     6961 tatcaccaaa aatatttaga aaagaatcct ggcggatact gccatatagg tgaattggaa
     7021 ttttacgagt tggagaaagc tcaaaaaaat tcaaagtata aggtaaaacc aaaaaaggaa
     7081 ttagagtcaa cactgacgga aatgcagtac aatgttactc aaaacaatgc tactgagccg
     7141 ccttttgata atgaatatta cgataatttt gaagatggta tatatgtgga tataacttcc
     7201 ggtgaacctt tgtttacatc taaggataaa tttgattcag gatgtgggtg gccaagtttc
     7261 tcaaagccca ttgatgaaaa agttgttaaa gagaaaagcg atttaagtta cggcatgata
     7321 agaacagaag taagaacgtc aaacagtgat tcgcatttag ggcatgtttt taatgatggt
     7381 cccaaagaat cgggcggatt aagatactgt atcaacagtg ctgcattgga attcattcct
     7441 gtagataaaa tggaagaaag aggatatgga gacttgcttt atttatttga ataaataaat
     7501 tcgctgtgtc atcctgagcc taagcgaagg atctcaggtt ttataaaatg agattcttcg
     7561 agctaatgct ctcaaaatga cacgggctta tagatagcaa tgacaaaaat gttaaaatat
     7621 tgtattgcat attatgacaa aattttatat gataacatac atatcataat aaattttgca
     7681 gctataaatg gagggtaaaa ataaattgac taaaacaaaa gaagttatat tatgcggatt
     7741 attactggca acaggtataa tactacccat gttttttcat atgtttggtt tgacaggtcc
     7801 tattgcttta ccgatgcata tacctgtact aatcggaggt tttttattgt cgccaaagct
     7861 tgcattgctc ttaggtataa taacaccggt cttgagcgga atgttaacag gaatgcctgt
     7921 aatgttccct atggctgtaa taatggcttt cgaattagga atatatggtt tagcagcatc
     7981 attagctgta agaaaattta acttatcagt tataccttca ttaattgttt ccatgatagc
     8041 aggaagaata gcagccggtt taacagttgc aatcttagtt gaattattcg gagtaaaaat
     8101 gaaccccctc atttatatca agggggcaat aataacaggt attcccggta tcataataca
     8161 attaatcttt ataccggcat tagtttatgc cataaaatct tatgtgaaaa ttaaatcggt
     8221 ctagttggaa atcatccttt caatccattc cttcaaaaca atggcatgat tgaattcttc
     8281 atttttggca gcatacaaca atgtaacatt tccttctttt aatttgtcct ttaataaatt
     8341 gataaaatca agggcatgtt cattgttatc cagctcaaaa ttatatctta ccctgaattc
     8401 atccattgta tccgggtcat gtttaaacgt cttccttagc tcagtgctgg gagtgatttc
     8461 ctttgcccat ttatgaatat ttattttttc tttgcttatt cctcttggcc aaagacggtc
     8521 taccaatatt ctgtaaccgt catcttcaga ggagtcttga tatactcttt taatcttaat
     8581 attatacata tcttttctcc ttttacttca ttattcattg gtgcctggca cctttggctt
     8641 attggtgcct ggcaccttcg tatggcgtct tcatatttat aatgacaatg gaagaaaata
     8701 ttatgataat tccaagcata tttataaaat ttaatccttc ttcgaacaca ataattccaa
     8761 tcagtgtagc aaccactatc tctactgatg caattacagg agtctttgag atttctattt
     8821 gctttctcaa ccctcccaag tataacaaat atgaccctac ggttggtatt agaccatacc
     8881 cgaacgaata gatccaaaat ttcagtccgg atacggtttt aatagactcc caaggtgaag
     8941 taaatattcc aatggcaatt ccgccaaata aaaagccgta aaatacaacc gtaaaaggat
     9001 gaatatcacc cgaggtagac tttcctatta tagttaccaa gctatacaaa aaacctgctg
     9061 ctacgccaaa aaatattccg gcggccgata attttatatt tgacagattc ccgcccgtaa
     9121 ccattaaaaa acatcctaca atatttataa ttaatgcaag aattttaatt cttcctattg
     9181 attccttgaa taacattctt gacatgatgg cgacaaaaac aggtgccgta tataaaagta
     9241 tggctgcagt ggcaattcct acgcttcgaa tgcttgtgat ataactgtaa ttaaaaagag
     9301 cctgggacaa tatgcctaac ataaagcatt ggctcaaccc ctttttgtca attttaaaca
     9361 agcttacgcc gcccttgtga agcattatgg ggatgagtat caaacatccc ataaacatcc
     9421 tcataaatgc cgttaatgtg gtgtcagcgc cggcactgtt taataaatta acaaacagtc
     9481 ctattgtacc ccacaagcaa cctgctgcaa aaatcatcaa atagccgata ttgtcgcgcg
     9541 taaaaatttt cttcttaaat tgaattattt tattcattgg tcagtctccg gtatttgtgt
     9601 cccggaagtg tctagaccgg tatttgccat actattaatg atatttctga attcatctgt
     9661 tacgctgtaa ccttggaatt tgactttgtt atattttttg ctgaattcat cgtagtattt
     9721 ttcttttaca tatacagtct tgttttcaat atcataatac aaatcaaagt tgtattcttc
     9781 ttctacactg aaaatcaata ctttgtcttt tgtaaaaata tcagcttcta tccaataata
     9841 gccgtcatcc tttgggggca gttcagctgt tacttctgtt ttttcggaga taagcagatt
     9901 ttcaagcaaa atttttacgt cgccggcctt gtccatatct tctgacagac ttccgtcgaa
     9961 agtcataatc ctttttatat tttcatgctg attggtatat ttaattcctt cattttcata
    10021 aatagttgca tcaattttgg aaacggtgct gattgaaccg gcaattcgag taatatcttt
    10081 ctccaatgct tcttcaacct ttgaaatata tctgttttta ctgaatattt tcattactag
    10141 cagtataata acaacaagta ccgcaattaa aaaagcaact cgtccgtaat taattcttct
    10201 tcttctgctg tacactcttt ttctcctggc catttcacca ccccatacct cttatttcaa
    10261 gcagagcttt tatattctcc tttagctctt caacagtgcc ttcgttattt ataactacat
    10321 cggccataaa tactttttct ttaatagata tttgcttatt tataattttt agtacatctt
    10381 cgggtttttt gttttctgca atggctcttt ccataagtct ttttctttga gtttcctcgg
    10441 aaacatatat caaccatatt tcgtcgtata tgggctcatg tatatctttg gtttcaaata
    10501 gcagtggtat gtctagaaat attacctttt ctttgcttgc ctcaatacct tcttttaatt
    10561 tgtcatatac atagccgtgc accatattat taagttcatt taatttttta tcgttgttaa
    10621 aaacgatttc tccaagcttt tgcctgtcta tttcatcacc ttttaatata tcctcgccaa
    10681 aatgattgat aattttgtaa tagagctcac ttccaatatt gtatccttca tggacaattt
    10741 tatccgaatc aagaaccgtg aacccaaact ccttaattat atcaacagct gtagattttc
    10801 ctgttccaat acttccggta atcactatga ccttaggtct attttgcttc ataccagcta
    10861 ctccccgtat taatatcaga ctttaagcga accttaaaat caacaactac attttccatt
    10921 tcttctgaaa gaattgtctt tacatcttca agttcatctt tgtatgcttc aataataagt
    10981 tcgtcatgaa cctgcaggat aagcctcgac ttcatcttgt tatttttaag ccttcggtaa
    11041 actccgacca tggctgcctt tataatatca gcagcggttc cttggacggg agtgtttaga
    11101 gcaattcttt ctccaaagga gcgtatgttg aaattacgtg atgtaagctc gggaatatac
    11161 cttctccttc caaaatatgt agttacataa ccgtcccttt taccctgctc cacaatatcc
    11221 ttcatgtatt tttctatatt tgaatagtat ttcatataat tatctatata tttttttgct
    11281 tcttttctcg ggatttttaa atctctggca agtccgtaat cacttatgcc gtacactatt
    11341 ccaaagttta cggctttggc tcttcctctc atctctgaag ttacttcgga taatttaaca
    11401 tggaaaacct gagaggcagt ctttgcatga atatctaacc cttgctcgaa tgcatctatt
    11461 aaattctgct catttgccaa gtgggacagt actctaagct caatttgcga gtagtcggca
    11521 tcgcaaagta tataatcatc ctccggcaca aatgcttttc tcagctctct tccttcttcg
    11581 gtgcgggtgg gaatattctg aaggtttggc tctgtacttg atattcttcc ggttgaagca
    11641 attgtttgat taaatattga atgcaccctg cccgttttct tgttcacttc gtttttcaat
    11701 ccttccacat aagtggagtt tagtttaacc atttgcctgt attcaataat tttatcaact
    11761 attgcatgct ctgagctcag tctttcaagc acttcaatat cggttgaaat accggtcttt
    11821 gttttcttta ttatcggcag acctaattta tcaaacaaaa taacggaaag ttgttttgga
    11881 gaattaatat tgaagctctc acctgcaata ttataaatat ccttttcaag ctccgcgatt
    11941 ttttcctgaa aatgaactcc taatttatca ataacgctta tatcgacttt aaagcccaca
    12001 aattccatgt aagccaaaac ttcaatcaat ggaagctcaa tttcttcata aagacaggtc
    12061 atcttaagct cgtctatatc ctcggttatt tgtttttgta ctttgcctat ggtgtttaag
    12121 taattgaata tatatgcctt cctttgttct atgtcaattt ccttgaatgt ttttctttta
    12181 actcccgtac caagaatttg attttctgaa ggaatttcca cattcaagaa ttcgtaggca
    12241 atcttgtcca atgaatagga gctttctgaa gggttcaaaa ggtattttcc tatttccgaa
    12301 tcaaattgga tattatttat ttgaatattg tttttcatta aatagataat ttcatttttt
    12361 aaattatgtc cccaaattgc gattttttcg ctttcaaata tttcttttaa ttcattaaga
    12421 actttttttt cttctgcttt ttcaaaatct atataataaa tattttcacc gtcggaaata
    12481 cctaaggcaa gagcattgct aaacagtgct ctttctccgt caagcaaaaa ttttaatatc
    12541 gctttctctt tgcggcttat atttttgatt atttctgtta atttttcttc tgagtcaata
    12601 taaataatgt caatattttt aactgtttca gccctgtcgg tatcaaacaa tgatatttgc
    12661 ccattatcat caacaggaat agtttcaaca agacgctttt taaactggcg gaattccagt
    12721 tcatcatata attctgacag tttcttaata tcaggtttct taattttgaa ttcctctata
    12781 ttaaattcaa tcggaatatc cgtaataata cgcgccaagg tctggctcat ataagcctgc
    12841 attttatccg tttccagttt ttctttaagc ttgcctgtaa tcttgttaat gttttcatat
    12901 aaattatcta agcttttata ctcctggata agcttaagag ctgtcttttc gcctactccc
    12961 gatacaccgg gtatattgtc ggaggaatct cccatgagag cctttaaatc aacaaattgt
    13021 tcaggtttta acccgtactc ttctcccatg gagtttatat ccatttccac aatatttgta
    13081 ataccttttt tagttaaaat tactcttaca ttttcatcta tgagctgcag gtagtccttg
    13141 tcactggtaa ccaggtaaac atctgctccc tgctcctttg cagcgcatgc aaatgtaccg
    13201 gatacatcat ctgcttcgaa gccttcaacc tctacccttt ttatattatg tacatcaaga
    13261 acatccctta taagtttaaa ctgctgaacc aattcatttg gtgctttttg acgatttgct
    13321 ttgtaatctt tatatttttc gtgcctgaat gtcggttttt cagggtcaaa agcaacacat
    13381 aaatattcag gggaatatgt atctataatt ttatacaaca tgctcaaaaa accgtataca
    13441 gcatttgtat attgtccttt ttttgtcttt aggggcggta atgcgtaaaa tgctctgaac
    13501 agcaggctgt tgccgtccaa tatcattatt tttttcatgg catctccttt atgaattgac
    13561 ccgatttctt gtgttttctt tattatatca acaaaaacca tgcgggtaaa ggaaaacttt
    13621 cagttcatat attcattttc ccggacatat aattaaatat atttatcgga ggtataaaaa
    13681 tgaaactgga gccaaaaaat tataactttt caaatgtaag atatttttcg ccgattcaat
    13741 taagcgagca ttatcagcta tacataaatt acatctcttg ccttaacaag gtaaacaacg
    13801 atttgaaaaa tgatctaata tatgatgaat gcaattccaa ctacagcgac attcgctctg
    13861 cccaaaaatc taaaaccttt tgttttgaca gcataaagct tcatgagtta tattttgaaa
    13921 atttaacagg gaacaatacc agaattcacg gtcctatcga aagagtactg gttaatactt
    13981 tcggttccta caataatttc cgcgaaaagt tcaaatgcat cggtatgagt atgagaggat
    14041 gggttttgct ttgttatgac aggtataaca atgaattcta tatatacggt caagactccc
    14101 atgattgcgg tgtaatgctt tatgcagagc ctttaatagt aatggacgtt tatgagcatg
    14161 cttatatgat tgattttgga acaaacagaa atgtatatat tgatgctttt ttccaaaaca
    14221 ttgattttaa tgtaattaat caacggttaa ataaaatttt gaaataatca cagtaactaa
    14281 agaaaccttc agacatataa tatcatacac acataaattt aaggaggtta attatgggat
    14341 tttttcatag aggtagagta gccggagcag gcggttcaaa tttactattt ttcttcttat
    14401 tattagttat actgtttgac gagtgctttg atgacatgaa gtgctagtaa ccctcgggac
    14461 agctacataa gctgtcccgt tctagttggt gcatactgtg tcattatttg aatggcgcta
    14521 attctaatat ttttttctca tattgtcttg tttttccttc aagtatatct gccttttctt
    14581 ttaatacttt tacaaagtct tcgtccttaa ttgaatccaa attaattcta atatttaata
    14641 tggctgacag cactccggtt cttgccatca ttgcaccaac caaggcatcc gtaacagaac
    14701 ttgaatttcc tcttaacact actgcttcca ccaaaggaaa tatttcatat gaagtttccg
    14761 caacctctaa tggtaccgat gctgcaactt ttaatccttc ttgaattctc tcgaggcgaa
    14821 ggcatttttc ttcttctgtt tcttttggaa gcttgtatgc ttccataacc ttcttgtatg
    14881 caaggctgtc ttcttcgata tagtttagaa gcctatctct aagctgagaa gctttattga
    14941 ttatagcatt catctcttct tttgaatcag catacttttt ctttgtaaca gttaaatttg
    15001 caaccatttc ggaaagtgca gcgcttaatg ctccgcacaa tgccgcaata cttcctcctc
    15061 cgggcacggg tgaatcagaa gcaactattt ttgcaaattc gtttattgtc aaatttttca
    15121 taataaccct cttacgattt taccgttttt tattacagtt tcaacaatat ttactgcaac
    15181 gtggtatgga agaaagtgaa ttgaaggata ttccaatata ataatatccg ccttcttacc
    15241 cttttctata cttcctcttg tatcacttct gcctaatgca gctgcagcat ttatagtcag
    15301 tgaagttata atttcttcaa ttgtcatatt catatgaaga gcagcaagag ctataataag
    15361 cggaatggag tttgtaaaac agctccccgg gttgaagtcc gtagccaaag aaacggcaca
    15421 tcccgaatca atcatgtatc ttgctcttgc atattcttcc ttaagacaaa atgccgttcc
    15481 cggaagaagg gttgcaactg tatcagactt agacatctgt cttattcctt catctgaagc
    15541 ctgcagcaaa tggtctgccg aagtgcagcc taactcaacg gctaattcag ctcctcccaa
    15601 ctgatacatt tcatctgcgt gaatttttaa ttttaaccct gaattctttg cggcagtcag
    15661 gaattttcgg gactgctcta ttgtaaatac atttttctcg caaaatatat ctgcaaattc
    15721 agctaagttt tcttccttaa ccttaggcag gacatcatct atcataaatt gaataaattc
    15781 atcttccttg cccttatatt ccggaagaac gctgtgaggt cccaaaaatg taggaacaat
    15841 atcaacagga tgcatatcgt ttaattgttt cattgcttta agctgtctta tttccgtttc
    15901 acaatcaagt ccgtatccgc tttttccctc caccgttgta acgcccatag ctaatatttt
    15961 atcaagtctg gttttcccca gagctgttaa ttcttctaag gaggtgttcc ttgtagctct
    16021 tacggagctt gttattcctc ctccgcgctc cattattgac atatagctgt cgccctttaa
    16081 tctccatgaa aattcatcgg ctctgtagcc tccgaaaatt aaatgtgtgt gggaatctat
    16141 aaatccgggc aatacggctt tatcttttgc atcaataatt tgatagtcat cttcatcata
    16201 attttttaat atgttcgagg ttccaacatc aactattata tcgtcttcta ttacaaggca
    16261 tccgttttca atcagcccta tatcagacat atccttgccg tgtttggggg cttttccctt
    16321 acatgtaaca agctcggatg cattttttat aatcaattta ctcattatta cctcctttat
    16381 gacaagggga cgggggtgtt gtcatcaaat gtccaccaag aaccgtcccc cctggtcatt
    16441 tataaccggg tttcgagaac ttggtcgtat ttaaagtttt ccaggcgcag atagtattct
    16501 gccgtatcca ccaaggcctg cattggaatc aaccctacaa tttcgcttcc gacaacattt
    16561 acaccatacc ttgccgcttc cattttgatt gtttcaaata cgcggtatat gcttgtcctg
    16621 gtataatcgg ttaagttcat tgatacttgt acaagacctc tgtcatgtat ttcaacgcct
    16681 atagctttta catatctaaa tcctccgctt atatgtctta cctgctttgc aattctattt
    16741 gcaacttcta tatcaggagt tgataaatta acattgaaag caatcagagg cattcttgca
    16801 ccaattgccg ttacgcctgc agtcggatgg atagtatcag gtccaaagtc cggcttccaa
    16861 agctcatcct tcattttttc tttcattcct tcaaactgtc cttttctcac attggcaaga
    16921 ttctctctgt ggggtgctga tgcagacttt tcataaagga aaaagggaag attatattta
    16981 tcggctgctt ccttagctac atcctttgct agtttatcgg cttcttcaac agtaacattt
    17041 cttacgggga tgaaaggaac cacatctacc gctcccattc ggggatgctg tccctggtga
    17101 taattcaggt caattagctc tactgccttg gcgatagctt caaccataac ttttttaatc
    17161 tcctcaggct ctcccacaac ggttaccact gtacggttgt ggttttcatc gcttgaatag
    17221 tcaagaagct tgactccttt ttttcctctg aaaacatcca gaattttttc tattttttct
    17281 gtatttctgc cctcactgaa attagggaca cattcaatta ttctatccat ttcgctctcc
    17341 cttatttttg taatttttta aggtttcttc aattaattca tcttttgcca aaaatggtat
    17401 tgtaatgtgg tcctctcctt caaatttttt gttgtattca atcgttgttt ctattgaatg
    17461 ctcgtttctt gcccagcttc ttcttgacac cccgaccatt gtatcccaag gaatggaagt
    17521 ttttattatt tcatcgactc ttttgcttcc gtcaagaaca agaccgaacc ccccgttaat
    17581 cgccttgctg acccctactc ctccgccgtt gtgaagtgca accaaggaca taccccttgc
    17641 tgcatttccc gcaaagcagt gaaccgccat atctgccgta acattgctgc cgtcatatat
    17701 gtttgatgtt tccctgaaag gagaatccgt tccgcctgta tcatgatggt ctctccccag
    17761 cattacaggg ccgatttcac cttttcttac catatcatta aatttaagtg ctatgtcacg
    17821 tcttcccaat gcatcttgat aaagaattct tgcttgggtt cctacaacta atttattttt
    17881 ctctgcatct ctaatccaaa tataattatc tctgtcctga cctcttctgt tagggtctat
    17941 cacagacatt gcagcttggt ctgttttatg taaatcttca ggctttccgc ttaagcaaac
    18001 ccacctgaag ggtccgtatc cgtaatcaaa aagcatcggg cccattatat cttccacata
    18061 ggaaggaaat ataaatcctt cgcttgtatc attgccgttc ttggcaattt ccttcactcc
    18121 agcatcaaat acagccttca tgaatgcatt gccgtaatcg aagaaatatg agcctctgtc
    18181 aaccaaagtt tttattgctt taaagtggtc agccaatgac ttatttacca tctgtacaaa
    18241 tttttctctg ttgctgttta ataattcagt tctttcttca aaggtaagtc ctttgggaca
    18301 ataacctccg tcataaggta catggcatga agtctggtcg gataacaaat ccacatgaat
    18361 gttgttatct gctgcatatt ccaacaagtc tacaatgtta ccgtggtatg ctattgaaat
    18421 tgtttgtttt ttgtacaggt actcccgtgc aatttcaaat gcttcctcaa gattatcggt
    18481 aaccgtggaa acccatcctt gttttaacct ggtttcaatt cttgagtagt caacctctgc
    18541 aattatacct acgccgttgg ctatttcaac agctttagcc tgtgcgccgc tcatccctcc
    18601 aagtcccgag gttatgaata aatgtcctgc caaatcttta tcatcagtga taccgagttc
    18661 ttttctgcct gcatttataa gtgtattaaa cgttccgtga acaataccct gaggtcctat
    18721 gtacatccag ccgcctgccg tcatttgccc atagcttgaa catcccattg cggttgcctt
//
LOCUS       DAOSHP010000033        18263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2485_length_18263_cov_9.752636, whole genome shotgun sequence.
ACCESSION   DAOSHP010000033 DAOSHP010000000
VERSION     DAOSHP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18263
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_18263_cov_9.752636"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1880
                     /gene="uvrA"
                     /locus_tag="PLB33_04850"
     CDS             <1..>1880
                     /gene="uvrA"
                     /locus_tag="PLB33_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOG62885.1"
                     /translation="IDKVLEYEEGTRIQVLAPMVRRAKGTHKKLLEDIKKEGFVRVRV
                     NGELFELEEDIILDKNKKHTIEVVIDRLIIKAGIQKRLVDSVETALTLSGGMVLIDLM
                     GQEELLMSTKFACTECGIGFGEISPRMFSFNNPIGACETCNGLGVKREIDVDLVIPDY
                     SKTIGERAIAPFGAEEDGYYYQMFAALAKYHNFDISKPLSQAPEEFIDELLYGTNGRE
                     VKVNFISKFQGAREYVREFEGVVNNLQRRYNETRSPDMRQWIEEYMYESPCPACKGAR
                     LNPISLAVKIGNKNIYEVTQLSVIKSLEFFDKLDLSPMNRIIGGQIIKEIRDRLQFLS
                     DVGLNYLTLARNASTLSGGESQRIRLATQIGSSLVGVLYVLDEPSIGLHQRDNAMLIK
                     TLRSLTDLGNTLIVVEHDEETMQNADYILDIGPGAGVHGGEVVEFGTIDEIKNNSNSI
                     TGQYLSGKKKIEVPEVRRKAEKFIEIKGAKENNLKNIDVKIPLGIMCCVTGVSGSGKS
                     SLINEILAKGLHQKLFKNTNRPGKFDEMLGIENIDKIITIDQSPIGRTPRSNPATYTG
                     VFDYIRDVYSMTLESKERGYSKGRFSFNVKGGRCEACRGDGILKIEMHFLPDVYVPCD
                     VC"
     assembly_gap    1881..1980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1981..2217
                     /locus_tag="PLB33_04855"
     CDS             <1981..2217
                     /locus_tag="PLB33_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62886.1"
                     /translation="MLIDVLNRLVEAGNTVIVIEHNLDVIKTADYIIDLGPEGGDGGG
                     TIVAQGTPEEVAEVKSSYTGQYLKKILWDSGDVP"
     gene            complement(2346..3713)
                     /locus_tag="PLB33_04860"
     CDS             complement(2346..3713)
                     /locus_tag="PLB33_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62887.1"
                     /translation="MVLTSLHYLYMIMVVIILVIMLMKKEIVIPCIVGIFAMGFLYSG
                     SAVFAVQSIFNTIIYSGNEFWGIILIISLVVAMSKSLQAVGADALIMSPIKKIMVNQT
                     MAFWGIGVAMMIISWLVWPSPAVPLIGAVLLPAAVATGLPVIYAAVAMNLFGHGIALS
                     SDFFIQGAPTITAAGAGIEVTEIIRASIPIWLTMSAVTIATAFFLMKRDLKKSSQLLP
                     SADNNFKHEEITQSKTAKYVAVLTPIAFLIDIAAMLKFNLAGGDATALVGGTAIIIML
                     LTVLTSFKLRDGFELIVNYIKEGFKFGISVFAPVLFIGGFFFLGNQNGATAVLGAGAP
                     GILNDISLFLSENIQMTKLSTVLTQTAVALITGLDGSGFSGLPIVGTLAYTFSNVIDI
                     DMAGLAALGQVITVWVDGGTIIPWAVIPVAAICNISPQELARKNLIPVLCGIGAAIAV
                     ALVIL"
     gene            complement(3821..4891)
                     /locus_tag="PLB33_04865"
     CDS             complement(3821..4891)
                     /locus_tag="PLB33_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62888.1"
                     /translation="MKEKHIFAGNNTSEGFFSYFDYLLKPEEAEHIYILKGGPGVGKN
                     RFMKKFADEMEKKNYSIEYIHCSSDNASLDGILIPELKILIVDGTSPHTIDPVIPGAV
                     DEIINLGAFLNVQKLNKHKTAIIQTNKAKSNKYLSAYRYLKAAGIIYNEISSIYDLYV
                     DVDKFNEMCEKAIAGLFAHSSDKKGGNVRKLFTESFTSNGYISYTEKFFEENEVWAVV
                     GNDTNYSSVFLERILNEAIKKGSTAECYYRPLFPKKLQHIYLPEKKLIIVTAENHMNE
                     KYKEVFDIHCIIDSESIKTRVSEIEKNLHLYDLLIRNALDKLSETKKLHELLEVFYVD
                     SMDFNLVNECFEKIVRGIGTQI"
     gene            5119..5307
                     /locus_tag="PLB33_04870"
     CDS             5119..5307
                     /locus_tag="PLB33_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOG62889.1"
                     /translation="MQGKVQKMDHHNPGIKCVVNTCYYYDEGDHCKAQKIEVQRRNAT
                     SSHETDCATFTKNDQSMT"
     gene            5479..6120
                     /locus_tag="PLB33_04875"
     CDS             5479..6120
                     /locus_tag="PLB33_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOG62890.1"
                     /translation="MKKITKIEYQKKNKERFNIYIDDEYGFAVDISILIKYSLKKGME
                     LDDALIDEILLSEEEISVYNYGISVLSRYFKSEYELRLKMKNKGFNPQLIDNAISILK
                     EHKYLDDERYCEMFINDKINISKHGVRKIKEALYYKGIDKEIIEEKIKCISAESEEER
                     ALLLGEKKLLNIKENDNRKKMSKLSNYLLGKGFEYETVNKTLRKLLNADNDFY"
     gene            6135..7022
                     /locus_tag="PLB33_04880"
     CDS             6135..7022
                     /locus_tag="PLB33_04880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG62891.1"
                     /translation="MKEFADYISDAFVHCRMTYNDGKFDIEALYANKQLEHITNKTMD
                     QLIGKKMTEVFPNLADSIFDWPKILCEAAMTSEHSVIEQYVNAFDKFVKFNIFGFKDD
                     TFYMTIQDLTHKREIRRLILEKDRQIEHLENEIKSRASIEPLTNIYNFQFITDCMKHS
                     IQSYKEEGTAFCVFLIDIDDFKEFNRKYGIETGDMVLQDVARILTLAARKIDVVGRYG
                     NDKFIMILNNVELDIAKIIVEKVKMEIGRYDLNISNNTIHASGAVVEYRGETLEEFVE
                     KAEELLKKAQAMGKGKILS"
     gene            complement(7044..8216)
                     /locus_tag="PLB33_04885"
     CDS             complement(7044..8216)
                     /locus_tag="PLB33_04885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOG62892.1"
                     /translation="MRNYIDLNKVCPVIKGRLSSSSNNIIPVIISYKSGKKVKEGQIS
                     SLSNRLRHNLPIINGCACEMSTESILKLTAEPDVEFISYDSKVFAVMEEARKTIGADY
                     SLNTKYTGKDVTVAIIDTGVAPHADLLYPENRIVGFKDFVNNRSKYYDDNGHGTHCAG
                     ILAGSGYSSKGKYRGIAPEANILSIKVLDENGNGNTSDILSTVQWIIETKEVYKTKII
                     NFSLGAIAQYKERRDPLVKAANKAIEENLIVVAAVGNSGPMHNTILSPATSRYVISVG
                     SLNDRGTSHFENETIAEFSSRGPTLDRVRKPDLIAPGVDIISLSNKNLSGYTALSGTS
                     MSAPMVSGAAALLLNKNPNYTHFDIKRILLNSCSKIKASSYEQGAGVLDMRRIFSQ"
     gene            8444..9127
                     /locus_tag="PLB33_04890"
     CDS             8444..9127
                     /locus_tag="PLB33_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG62893.1"
                     /translation="MPKILVVDDERPIAEIIKYNLQKEGFEVQTAYDGDEAIKAVHNF
                     QPELIILDIMLPKKSGFDVLKEIRMEYVMPVIMLTAKDEENDKVTGLELGADDYITKP
                     FSNKELIARVKANLRRVKLSKVEEEMKSKIIAVGNLTIDMNTYEVFKDGEIIDLTNRE
                     FDLLKYLFQNSDRVFNREHLLKEVWGYEFGDLRTVDVTVRRLREKIETEDEKYIITKR
                     GTGYYFKSK"
     gene            9162..10943
                     /locus_tag="PLB33_04895"
     CDS             9162..10943
                     /locus_tag="PLB33_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG62894.1"
                     /translation="MFKSIRWRFILIYFLLVFLAMSIVGIYIVNQLEGIQLNMNIKNM
                     DARIRSIIQSYSSLNTGNWDENIEEIQKSIGTVQVGYNENIYVILNDVNRTIIAGSVE
                     ESIGISAFNYNKIDNYILTKSMDGTPYHIAPAEQFEDTENQRFYYHMSFPVQTEGNIK
                     GYIYLVNNIDYIYDTVDESKQIMTQATIIALTLTIFLGLILSTSITGPIKELTIKARQ
                     MSLGDFEQKVNVKSDDEIGQLGNMFNYLIDQLKKSMSSLQQEKSKMETTFTYMADGVL
                     TVDLSGNIIHANPVAKNILSLKNADEKFDDVIVNLKDSISIDYIKSKGYRGTDLAEKN
                     GETYNIDYAPFMNNKNEIGGVILVFKNITEQHRIDKLQKEFVANVSHELKTPITTIKS
                     YAETLLDGALEDKQISEEFLNVINSESDRMTRLVSDLLRLSKMDYEQTNWNKERINLL
                     DMVNIVIKKLQIQIKEKNINIHLNIPDDINIMFDRDGFEQILLNIAGNAVKYSYEKGN
                     VWISARRSKKNIVVSIKDDGQGIPTEDQDRIFERFYRVDKARARGLGGTGLGLSIAKE
                     IAEAHNCTIAVNSRIDEGTEMIITIPE"
     gene            10954..12357
                     /locus_tag="PLB33_04900"
     CDS             10954..12357
                     /locus_tag="PLB33_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62895.1"
                     /translation="MLNIKIKNSILLVMVLICIYLSSNVWLKLPEFFTPKAYEDKRAE
                     ETEADIWKVLRPVKNVIKYDDNYTVLYSDNKGLWQEALNVIYDAFNATNSTISETAAF
                     PAEYLKFDFNTNIPVEIFTTHMKIENNNINNTVKNIKNVTIDLKDNNSIYIYNGENNI
                     KIKSNQINSKKLTDIVLSINFENKTKYSMPEKVGDETIQVPVPLEQSALNPVFVQSEL
                     DVFDIDRINEIAKDYFKNDYDYVRKSVEVSGNLVYVYRTEKILKINEDGLLDFYDASI
                     EPENSPNVYKSFAAAVNFIEEFLGFPENGYLSNVEKILLEGNEGYRFTFSYNILERPI
                     LFSRVRENAALQIDVAGNNVISYKRFIRNIDRTQMDKMAEIEVTPAIEVIRNNIDTSA
                     GEENADTVPGEGQEEAVQLKPIKPEMIKEINNIYLGYFDLSRISREQVLRVVWVIETN
                     EKTFIFNAISGLLIEEW"
     gene            12370..13200
                     /locus_tag="PLB33_04905"
     CDS             12370..13200
                     /locus_tag="PLB33_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62896.1"
                     /translation="MDWNKTNTILITAFIILNVFLFVSSKNIRNAEHKPNYDKEFAQE
                     VAYLLEEKNIKINVEIPNNIYILPVLETEYEIIKIDSNLLTNFLNERIEPFEDVYKYT
                     NSKGETLEIIDGKKLRLTLRNKVDDKINEDSKIQEEINNFLKEKNIDASGYVESFKHT
                     SENECIYAYTLKYNDYSIDNSYMYFFVDNEGIYKFEMQRIISVDEIPEKIRTIYAIEA
                     LPRILTYPEIKNKEIIKIEMTYYSAEDENWHNIERINSDPTWKVIFSDGTQIHLPGIE
                     "
     gene            13221..14480
                     /locus_tag="PLB33_04910"
     CDS             13221..14480
                     /locus_tag="PLB33_04910"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG62897.1"
                     /translation="MSKFVIEGGTKLKGKVNIGGFKNAAVAIIPATLLCPERCIIENV
                     PKIQDVNVFLEILRELGAEADYINDTSIYVDASNVKNCYLENGLSKKMRASYYLLGAG
                     LGRFNGASCLMPGGCDIGERPIDQHIKGFEALGGKVTLTPDGRISVQADRLVGAKIYL
                     DVVSVGATINIMLAAVYAEGRTIIENAAKEPHIVDTANFLNSMGADIKGAGTDVIRIN
                     GVKELKGCTYKVIPDQIEAGTYMIAAAATKGDIIIDDIIPKHMEPITAKLKEMGMGIE
                     EYGDSMRVYYEKELVPVNIQTLPYPGFPTDLQQPMTVLLASLCGESTVVEGVSQDRFR
                     YVEELKKMGACIEFTKKQARIKGTKNLRGTVVQATDLRAGAALIIAGLIADGLTEVTN
                     IQHIDRGYEHIENKFMNLGAKIKRVED"
     gene            14500..>15578
                     /locus_tag="PLB33_04915"
     CDS             14500..>15578
                     /locus_tag="PLB33_04915"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOG62898.1"
                     /translation="MNKAVLIPDSFKGTLSSIEICTIISDKIYKHFPHCHVVSIPVAD
                     GGEGSVDCFLSALGGEKIVETVSSPYFEDMESFYGLIDDTAVIEMAACAGLPLVEDRK
                     NPSLTTTYGVGQLIMSAAKKGCKKIIVGLGGSSTNDGGCGAAAAVGVKFYDKKGNEFI
                     PIGKTLIDIDRIDLSHKAKELDNIEIITMCDIDNPMYGPTGAAYIFGPQKGADEKMIL
                     ELDKGLKNLCDVIERETGKDLKDVPGSGAAGAMGAGMIAFFNSVLQMGIETVLDTVKF
                     DEIIEDADMIFTGEGRIDSQSLRGKVVIGVGKRAKNKNIPVTVIAGGAANNIDDAYDM
                     GVTSIFTINRLPEDFEISRHKSKEN"
     assembly_gap    15579..15678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15679..16477
                     /locus_tag="PLB33_04920"
     CDS             <15679..16477
                     /locus_tag="PLB33_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62899.1"
                     /translation="DISSMEIAENVDYDILFELTPLEIFSGQPAIDHIKAALSRKKHA
                     VSANKGPIAWAYESLRDLAEKNKVLFYHETTVMDGTPLFNLVDETLKFCKIKEVKGIL
                     NSTTNFVLEEIAKGKPYKEVIEEGKKRGFVEEDPAMDIEGWDAAAKTAALLNVLMGAN
                     ITPFDVDRKGIEDIDYEQIKEAEKRGNMIKLICYGGIEDGKVVARVKPEEVNKNSLLA
                     TITGTTSIVSITTDLMGTISVVEHEPEIEQTAYGVFSDLVRVLSNKN"
     gene            16530..18200
                     /locus_tag="PLB33_04925"
     CDS             16530..18200
                     /locus_tag="PLB33_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG62900.1"
                     /translation="MANINNILKGIEKDIEDILFAYVKTESFTNTINEKKAEEFFLDY
                     FSKIPYFKNTPGSYGIYEIENDALNRNVSYAFLKGRGQDTIVFIHHNDIVGVEDFKLL
                     KKYAFSPKELKAQLLRIKNTLSEDAKKDLEDDTFIFGRGVCDMKGGGSIQIALLKRYS
                     ELENFNGNIVLIAVPDEENLSAGMRSAVRLLSELQDKYNLKYKLMINTEPHQRKDDET
                     GVFSEGSVGKIMPFVYVRGYLAHAGKVFEGLNPIHIMSKVVSRTELNMDFSDVVGKEA
                     APPPTWLYLKDSKEQYDVSMPLSVKGCFGILTLNQTPQSILEKVKNICEESFDEVIDD
                     MNNSFRRFAKATGLPLKYLQWESKVVNFQELFKEAEAAYGDEFIREYNKELEILGKKL
                     KGNEISIIHCNFELIEFIYGYIDDIKPRIVYGLIPPYYPNVSNLFIDDLDENVSSLCD
                     NLIKFTQEEFNQNYNTEYFYTGISDLSYSSIEKSGEIYKALEESMPLLGQFYNVPVEL
                     IEKISMPCINIGPWGKDFHKMTERVNKEDLYIRTPRIINKAISLILGN"
BASE COUNT         6530 a         2710 c         3633 g         5190 t
ORIGIN      
        1 attgacaaag tattggaata tgaagaagga acaagaattc aagttttagc tccaatggtg
       61 agacgtgcaa agggaactca taaaaaatta ttggaggaca taaaaaaaga aggctttgtc
      121 agagtgaggg ttaacggaga actttttgaa ttagaagaag atataatatt ggataaaaat
      181 aaaaaacata ctattgaagt tgttatagac aggcttatta ttaaagccgg aatacaaaaa
      241 aggcttgtgg attcggtgga aacggccttg acattatccg gagggatggt tctgattgac
      301 ttaatgggtc aggaagagtt attaatgagc acgaaatttg catgcacgga atgcggcata
      361 ggttttggag aaatttcccc gaggatgttt tctttcaata accccatagg agcatgtgaa
      421 acatgcaacg gtcttggtgt taaaagagaa atagatgttg acttggtaat tcccgattat
      481 tcaaagacaa taggcgaaag agcaatagca ccttttggtg cggaagaaga cggatactat
      541 tatcaaatgt ttgcagcgct ggcgaaatac cataattttg atatatccaa acctctttcg
      601 caagctccag aagagttcat cgatgaactt ttgtacggta caaacggaag agaagtaaaa
      661 gtaaatttta tcagcaaatt tcaaggagca agagaatatg tcagagaatt tgaaggagtt
      721 gtaaacaatc tgcaaagaag atataacgaa accagatctc ctgacatgag acaatggata
      781 gaggaatata tgtatgaaag cccttgccct gcatgtaagg gagcaaggtt aaatcctata
      841 agccttgcag taaaaatagg taacaaaaac atatatgaag taactcagct ctcagttata
      901 aaaagcctgg agttttttga taagctggac ttgagtccaa tgaatagaat aatcggcgga
      961 cagataatca aagaaattcg agacagattg caatttttgt cagatgttgg acttaattat
     1021 ctcacattgg caagaaatgc atctacgttg tcaggaggcg aatctcagcg aataagattg
     1081 gccactcaaa taggttcaag tcttgttggg gtattatatg tattggatga gccaagcata
     1141 ggactccatc aaagagataa tgcaatgctt attaaaacat tgagaagttt aacagattta
     1201 ggtaatacgc ttatagtggt tgagcatgat gaagaaacta tgcaaaatgc agattatatt
     1261 ttggatatag gtcccggagc aggagtgcac ggcggggaag tagtagaatt tggaactata
     1321 gatgaaatta aaaataattc aaattcaata accggacaat atttgagcgg aaagaaaaaa
     1381 atcgaagtgc ctgaagtaag aagaaaggct gaaaaattta ttgaaattaa aggggctaaa
     1441 gaaaataacc ttaagaatat agacgtgaag atacctttag ggataatgtg ctgcgttaca
     1501 ggggtatccg gctccggtaa aagctccttg ataaatgaaa tattggcaaa gggtcttcac
     1561 cagaaattat tcaagaacac aaatcgtccc ggaaaattcg acgaaatgct tggcattgaa
     1621 aatatagata aaataattac cattgaccaa tcaccgatag gaaggacccc caggtctaat
     1681 cctgccacat acacgggagt atttgactac atcagggatg tttattcgat gactcttgaa
     1741 tctaaggaaa gaggctatag taaagggaga ttcagtttta atgttaaggg cggtagatgc
     1801 gaagcatgcc gcggggatgg aattttaaaa attgaaatgc attttctgcc tgatgtatat
     1861 gtgccctgcg atgtgtgcaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 atgcttatag atgttttgaa cagacttgtg gaagcaggca atacggtaat tgtaatcgag
     2041 cataatttag atgttataaa aactgcagat tatattatag atttaggccc agagggcggt
     2101 gacggcggag gcacaatcgt agcacaaggt actcctgagg aagtagccga agtaaaaagc
     2161 tcttatacag ggcaatactt gaaaaagatt ttatgggaca gtggggacgt accttaactg
     2221 tcccattgct atgaatgacc ttaaattaca ataaattaaa gtggctaagg attattgtca
     2281 tatatgacag taggcttagc catatttgtt aagtgggaca gttaaggtac gtccccactg
     2341 tccttctaaa gtattactaa ggcgactgca attgcagcac cgattccgca aagcaccggt
     2401 attaaatttt ttcttgccag ctcctgagga cttatattac aaatcgcagc cacaggaata
     2461 acagcccaag gaattatggt tcctccgtca acccatactg ttattacttg acctaaagca
     2521 gccaatcctg ccatatcaat atcgataaca tttgaaaatg tataagccaa ggttcctaca
     2581 atcggaagtc ctgaaaatcc tgaaccatcc aaacctgtta tgagggctac ggcggtctga
     2641 gttaaaacag tcgaaagttt ggtcatttgt atgttttcag ataagaataa acttatatca
     2701 tttaatatcc ccggtgctcc tgcacctaat actgctgtag ctccattctg attacctaag
     2761 aagaaaaatc ctccgatgaa aagtacggga gcaaaaacag aaattccaaa tttaaaacct
     2821 tcttttatat aatttactat caactcaaaa ccatctctta atttaaagct tgttaaaaca
     2881 gttaagagca ttatgattat tgcggttcct cctacaagtg cggttgcatc gccgcctgcc
     2941 aaattgaatt ttaacattgc cgcaatatca attaaaaatg ctatcggagt taaaacagcc
     3001 acatattttg ctgtcttgct ttgagttatt tcttcatgtt taaaattatt atctgcagaa
     3061 ggtaaaagtt gggaagactt tttcaggtct cttttcatca ggaaaaatgc agttgctata
     3121 gtcactgcag acattgtaag ccatatggga attgatgccc taataatttc agttacttca
     3181 atgcccgccc cggcagcggt tatggtaggt gctccttgta taaaaaagtc actggaaagt
     3241 gcaataccat gaccgaataa attcatggca accgccgcat aaataacagg caggcccgtg
     3301 gcaactgcag caggcagaag gactgcccct attaagggaa ctgcaggact gggccaaacg
     3361 agccaggata ttatcatcat tgccacacct attccccaaa atgccattgt ttgattaacc
     3421 attatttttt tgattggtga cataattaac gcatctgcac caaccgcctg caaggactta
     3481 gacatggcga caaccaatga aattataagt ataatacccc aaaattcatt gcctgaataa
     3541 ataattgtgt taaatataga ctgaacagca aataccgcac tgccagagta aaggaaaccc
     3601 atggcaaaaa taccgacaat acatggaatt acaatttctt ttttcatgag cataataaca
     3661 agaataatta ctaccattat catatataag tagtgaagag aagttaaaac catatgaaac
     3721 ctccttggaa ttaagcagag gaacattcct tcaagaaaat tttaatagtg tgtccccctg
     3781 ccttatgtaa ttgtgtcctc ttacatataa attggcacca ttatatttgt gtacctattc
     3841 ctctgactat tttttcaaag cattcattaa caaggttaaa atccatggag tcaacataaa
     3901 atacttctaa aagttcatgc aacttttttg tttcggaaag cttatctaag gcatttctta
     3961 ttaataaatc atataaatga aggttttttt caatttcaga aacacgtgtc tttatacttt
     4021 cagaatctat tatgcagtga atatcaaaaa cctccttata tttttcattc atatgatttt
     4081 ctgcagtaac aattataagt tttttttcag gcaagtatat atgctgcagc tttttcggga
     4141 atagaggtct gtaatagcat tctgcagtag atcccttttt tattgcttcg tttaaaattc
     4201 tttccaggaa tacggaagaa tagtttgtat cattacctac aaccgcccac acttcatttt
     4261 cttcaaagaa cttttcggta tagcttatat atccgtttga cgtaaaagat tcggtaaaca
     4321 gttttctgac attgccgcct tttttatcag acgaatgagc aaacagtccg gcaattgctt
     4381 tttcgcacat ttcattaaac ttatcaacat ccacatacaa atcataaatt gagctgattt
     4441 cgttatagat aatacctgca gctttcaaat atctgtaagc acttaaatat ttatttgatt
     4501 tggctttatt ggtttggatt atggctgtct tatgtttatt aagtttttga acatttaaaa
     4561 aggctccgag attaattatt tcatcaactg ctcccggaat gacagggtca atggtatgag
     4621 gtgatgtacc gtcaacaata agaattttta gttccggaat caaaattccg tcaagacttg
     4681 cattgtcgct tgagcagtgt atgtattcta ttgaatagtt ctttttttcc atttcatctg
     4741 caaatttttt catgaaacga tttttgccta ctcctggtcc tcctttaaga atataaatgt
     4801 gttcagcttc ttccggcttt aatagataat caaaatagct gaaaaatcct tcagatgtat
     4861 tgttgccggc aaaaatatgt ttttctttca ttttctacct cctcaatgtt attccattat
     4921 atgaacgatt tctgttcttg ttaaaattaa ttctttttta ttattcctga ttacatcctg
     4981 aaagattaag cataaggaga aattcttctt tagatttgta tttatttgta ataaaaaaat
     5041 agatattata aaaaaaatat taaagggcat aataattttg aaagttttga ctttcaataa
     5101 caaaaaagga gacatattat gcaaggaaaa gtacaaaaaa tggatcatca taatcccggc
     5161 atcaaatgtg ttgtaaatac atgttattac tatgatgaag gggaccactg caaggcacag
     5221 aagattgaag tgcaaaggcg caatgcaact tcatcacatg aaacggattg tgcaacattt
     5281 actaaaaacg accaaagcat gacataaagg tgccataaca ggcaagataa tttatccgaa
     5341 aaattgtcct tttaagagga caattttttt gggtattatt tcggatactt ttttatctta
     5401 gatatgccca tataatattg ctttataaat attgatattc ttcaaaatct gtgttaatat
     5461 gtagaaaatg agggaaatat gaaaaagatt accaaaattg aataccagaa gaaaaataaa
     5521 gaaagattca atatatatat agatgatgaa tatgggtttg ctgttgatat aagcatattg
     5581 attaaatatt cgctcaaaaa aggtatggaa ttagatgatg ctctcattga tgaaatattg
     5641 ttatcagaag aagaaataag tgtatataat tacggaattt ctgtgctttc aagatatttt
     5701 aaaagcgaat atgaattaag gcttaaaatg aaaaataaag gctttaaccc tcagttaatc
     5761 gacaatgcca tcagcatact taaagagcat aaatacttag atgatgaaag atattgtgaa
     5821 atgttcataa acgataaaat caacatttcc aaacatggtg tgcgtaaaat taaggaagct
     5881 ttatattaca agggcattga caaagaaata atagaagaaa agataaagtg tatttcagca
     5941 gagagcgaag aagaaagagc tttattattg ggagagaaaa aacttttgaa cataaaagaa
     6001 aatgataacc gcaaaaaaat gtcaaagttg tcgaattatt tgttaggaaa gggctttgaa
     6061 tatgaaacgg taaataagac actaagaaag ctattaaatg cagacaatga tttttactaa
     6121 agggggctat acttatgaag gaatttgctg attatatatc cgatgcattt gttcattgca
     6181 ggatgaccta taatgatgga aaatttgata ttgaagctct ctatgcaaat aagcagttag
     6241 agcatattac caataaaaca atggaccagt taatcgggaa aaaaatgacg gaagtatttc
     6301 ctaaccttgc cgattcaata tttgactggc cgaaaatttt atgcgaagct gcaatgacaa
     6361 gtgaacactc ggtaattgaa caatacgtca atgcctttga taagttcgta aaattcaata
     6421 tatttggatt taaagatgat actttttata tgacaattca ggatttaact cataaaagag
     6481 aaatcaggag attaatactt gaaaaagaca gacaaatcga acatttggaa aatgaaatca
     6541 aatcaagagc aagcatagag ccccttacca atatatataa ctttcaattt attaccgact
     6601 gtatgaaaca cagcatacaa agctataagg aagaaggaac ggcattttgc gtattcttga
     6661 tagatattga tgactttaaa gagtttaatc gtaaatacgg cattgaaaca ggagatatgg
     6721 ttttacaaga tgtagcccgc atactcactt tggctgcaag aaaaatagat gtggttggca
     6781 ggtatggaaa tgataaattt attatgatat tgaacaatgt agaacttgat attgcaaaaa
     6841 taattgttga aaaagtaaaa atggaaatcg gcagatatga tttgaatata agcaataata
     6901 ctattcatgc aagcggagcc gttgtggaat acaggggcga aacattggaa gaatttgttg
     6961 aaaaagctga agaattactt aaaaaagcac aagctatggg aaagggaaaa atattgtcat
     7021 aagggagcat tgcgctccct ttattactgt gaaaaaatgc gtctcatatc tagaactcct
     7081 gctccttgct catatgaaga tgcttttatt tttgagcatg aattaaggag tattctctta
     7141 atgtcgaaat gggtgtaatt gggatttttg tttaaaagta aagcggcagc tccggatacc
     7201 attggggcgg acattgatgt accgctcaat gcggtgtagc cgcttaagtt tttattggat
     7261 aaagatatta tatcaactcc gggagctatt aaatccggtt tgcggactct gtccaatgta
     7321 gggcctctgc ttgaaaattc agcaattgtc tcattttcaa aatgagacgt gcccctgtca
     7381 tttaatgagc ctacggaaat tacatacctg ctggttgcag gagataatat tgtattgtgc
     7441 ataggcccgc tgttgccaac ggcagcaacc actatgagat tttcttcaat tgctttgttt
     7501 gccgccttta ccaatggatc tctcctttcc ttatactgag cgattgcgcc gagagaaaaa
     7561 ttgattattt tagttttata aacctccttg gtttcaataa tccattgaac tgtagataga
     7621 atatccgatg tatttccgtt tccgttttca tcaagaacct tgattgatag tatattggct
     7681 tccggggcta ttcctctata tttgcccttg gaagaataac cgcttcctgc aagtattcct
     7741 gcgcaatgtg ttccatgacc gttatcatcg taatacttgg agcgattgtt tacaaaatcc
     7801 ttaaagccaa ctatcctgtt ttcgggatat aacaaatcag cgtgaggggc gacacctgta
     7861 tcaattattg caacagttac atcttttccc gtatatttag tatttaggga atagtcagct
     7921 ccgattgttt ttcttgcctc ctccattacc gcaaatactt ttgaatcata tgatataaat
     7981 tcaacatcgg gctcagctgt taatttaaga attgattcag tgctcatttc acaagcacaa
     8041 ccgttgataa tgggcagatt gtgccttaat ctgtttgata gcgaactgat ttgcccctcc
     8101 tttacttttt tgccggattt ataacttatg ataacaggta taatattatt gctgcttgaa
     8161 gaaaggcgtc cttttataac cggacacact ttatttaaat ctatataatt tctcataaaa
     8221 ttcacctacc taaattaatt tatgctttta aataaagatg gtgttcattt ttgaaaaaca
     8281 tgttataata ttcatgaata atataatggg aaagatatct tcctccttag gaggcgggta
     8341 tatgtgaaat tcaggcgggt atatcccgcc tcgttgaaac ggtgaaattg cacggcaatt
     8401 tcttcggatg tttaaagaca gtaattaaaa taggggtgaa ataatgccta aaatattagt
     8461 agtggatgat gaaagaccta tagccgaaat aattaaatac aatcttcaaa aagaaggctt
     8521 tgaagtgcag acagcctatg acggggatga agctataaag gcagtgcaca atttccagcc
     8581 tgaattaata atacttgata ttatgctgcc aaagaaaagc ggatttgatg ttttaaaaga
     8641 aatacgcatg gaatacgtaa tgcctgttat tatgcttact gcaaaagatg aagaaaacga
     8701 caaggttacg gggcttgaat tgggagcaga cgactatata accaagcctt tcagcaacaa
     8761 ggaattaatt gcaagagtca aggctaattt gagaagggtg aagctttcaa aggttgaaga
     8821 agaaatgaaa tcaaagataa tagcagtagg aaatctaacc attgacatga acacatatga
     8881 agttttcaaa gatggtgaaa taatagatct tacaaacagg gaatttgacc ttttaaaata
     8941 tttgtttcaa aattcagaca gagtatttaa cagagagcac cttcttaagg aagtatgggg
     9001 atatgaattt ggtgatttaa gaacagtcga tgtgacagtg agaagattaa gagaaaaaat
     9061 agaaacagaa gatgaaaaat atataatcac aaaacgaggg acaggatatt attttaaaag
     9121 caaatgatgc caaattttgc tgcgtattct aaggagaaaa aatgttcaag agcataagat
     9181 ggagatttat tttaatttat tttttgttgg tttttcttgc tatgtcaata gtcggcatct
     9241 atatagtcaa tcagcttgag ggaattcagc ttaacatgaa tataaaaaac atggatgctc
     9301 gaattcgctc aattattcaa tcctacagct cattaaatac cggtaactgg gatgagaata
     9361 ttgaagaaat tcaaaaaagc ataggcactg tacaggtagg ttataacgaa aatatatatg
     9421 taatcttaaa tgatgtaaac agaacaatta ttgcaggcag tgtagaagaa tcgattggca
     9481 taagtgcctt taactataat aaaatagaca attatatact gactaaatcc atggatggaa
     9541 ctccttatca cattgcaccg gcagaacaat ttgaagatac cgaaaatcaa aggttttatt
     9601 atcacatgtc ttttccggtg cagacggaag gaaacatcaa aggttatata tatttagtaa
     9661 ataatattga ttatatttac gacacggttg acgaatcaaa acaaattatg acacaagcta
     9721 ccataattgc cttaacctta acgatatttt taggactgat actttcaaca agcataacgg
     9781 gaccaatcaa ggagctcact ataaaggcaa ggcagatgtc attaggcgat tttgagcaga
     9841 aggtaaatgt aaaatcagat gatgaaatag gtcaattggg caatatgttc aattacctta
     9901 tagaccagct gaaaaagtca atgtcaagcc ttcagcagga aaaaagcaag atggaaacca
     9961 ccttcacata tatggcagat ggagttttga cagttgattt atcaggtaat attatacacg
    10021 caaaccccgt tgcaaaaaat atattatcac taaaaaatgc cgatgagaaa tttgatgatg
    10081 taatagtaaa tctcaaagac agcatatcaa ttgactatat taagtctaaa ggttatcgcg
    10141 gcacagactt ggcagaaaaa aacggagaaa cttataatat agactacgca cctttcatga
    10201 acaacaaaaa tgaaataggg ggcgtgattc ttgtgtttaa aaatattacc gagcagcatc
    10261 gaatagataa gcttcaaaaa gaatttgtcg caaatgtatc gcatgaatta aaaacgccga
    10321 tcactaccat aaaaagttat gcagaaactc tattggacgg tgcattggaa gataaacaaa
    10381 tatccgaaga atttctgaat gtcataaatt ccgaaagcga taggatgaca aggcttgtca
    10441 gcgatttatt gcgcctttca aagatggatt atgagcagac aaattggaat aaagaaagaa
    10501 tcaatttgct ggacatggtt aatatagtca tcaaaaaact gcaaattcaa ataaaagaaa
    10561 aaaatataaa tattcattta aatattccgg atgatattaa tataatgttt gacagggacg
    10621 gatttgagca aatccttttg aatattgcag gaaatgcagt taaatattca tacgaaaagg
    10681 gaaatgtatg gattagcgct agaaggagca agaaaaatat agtcgttagc atcaaagatg
    10741 acgggcaggg tattccgaca gaagaccagg atagaatttt tgaaagattc tatcgtgttg
    10801 ataaagcaag agccagaggc ttgggaggaa ccggccttgg actttcaatt gccaaagaga
    10861 ttgcagaagc ccacaactgt acaatagccg tcaacagccg gatagatgaa ggaactgaaa
    10921 tgattattac aattcctgaa taaagaggac actatgttaa atataaaaat aaaaaattca
    10981 atattattgg tcatggtact catatgtata tacttaagca gcaatgtatg gctaaaactt
    11041 cctgaattct tcacacctaa ggcatatgaa gataaaagag cggaagaaac agaagcagac
    11101 atatggaaag tattgagacc tgtaaaaaat gtaattaaat atgatgataa ctatactgtt
    11161 ttgtattcag ataataaagg gttgtggcag gaagcattaa atgtaatata tgatgctttt
    11221 aatgctacaa atagcaccat ttcggaaaca gcggcatttc cggctgaata tttgaaattt
    11281 gatttcaata cgaatatccc tgtggaaata tttacaaccc atatgaaaat tgaaaacaac
    11341 aatataaaca atactgtaaa aaatataaag aatgttacga tagatttgaa agataacaat
    11401 tcaatttata tttacaatgg cgaaaacaat ataaaaatta agagtaatca aattaattcc
    11461 aagaaattaa cagatattgt gctatctata aattttgaaa ataaaacaaa atattccatg
    11521 ccggaaaaag taggggatga aacaatccag gtacccgttc ccttagagca gtcagcgctg
    11581 aatccggtat ttgttcagtc ggagcttgat gtatttgata ttgacagaat taatgaaatt
    11641 gccaaagatt attttaaaaa tgattacgac tatgttagaa aatctgttga agtaagcggg
    11701 aatttggtat atgtttacag gaccgaaaag atattaaaaa ttaacgagga tggactctta
    11761 gacttctacg atgcatcaat cgagccggaa aacagtccga acgtttataa aagctttgct
    11821 gcagccgtaa attttatcga agaatttctg gggtttccgg aaaacgggta tttgagcaat
    11881 gtagaaaaga ttctcttgga aggaaatgaa ggttataggt ttacattctc ctacaatata
    11941 ttggaaaggc ccatattgtt cagcagggta agagaaaatg ctgctcttca aattgatgtt
    12001 gcaggaaaca atgttatttc atacaaaagg tttatcagaa atattgatag aacacagatg
    12061 gacaaaatgg ctgagataga ggttactcct gctattgaag tgataagaaa caatatcgac
    12121 acttcggccg gtgaagaaaa tgcagataca gtacccgggg aaggccagga agaagctgta
    12181 cagcttaaac ccattaagcc ggaaatgatt aaggagatta acaatattta tcttggatat
    12241 tttgatttat ctcgaatttc aagggaacag gttttaaggg tggtatgggt tattgaaaca
    12301 aatgaaaaaa catttatatt taatgccata tcaggtcttt taattgaaga atggtaacag
    12361 gtgaggcaaa tggattggaa taaaacaaac acaatattaa taacagcttt tataatttta
    12421 aatgtttttc tttttgtgtc ttccaagaat attagaaatg cagagcacaa gcctaattat
    12481 gataaggaat ttgcccaaga ggtagcatat ttattagaag aaaaaaatat aaaaattaat
    12541 gttgaaatac cgaataatat atatatactg cctgttttgg aaacagaata tgaaataatt
    12601 aagatagatt caaatctttt gacaaatttc ttaaatgaaa gaattgagcc ttttgaagat
    12661 gtgtacaaat acacgaacag caaaggagaa actttagaaa taattgacgg caagaaatta
    12721 agactcacat tacgcaataa ggttgacgat aaaataaacg aagattcaaa aatacaagaa
    12781 gaaataaaca attttttaaa agaaaaaaat attgatgcat ccggatatgt tgaaagtttc
    12841 aaacacactt ccgaaaatga atgtatttat gcatatacgc taaaatataa tgactattca
    12901 atagacaatt cctacatgta tttttttgta gataatgaag gaatttataa atttgaaatg
    12961 cagagaataa tttcagttga tgaaataccg gaaaagattc gtacaatata tgctattgaa
    13021 gcattaccgc gaattttaac ttatccggaa ataaaaaaca aagaaattat taaaattgaa
    13081 atgacatatt atagcgctga agatgaaaat tggcataata ttgaaaggat aaattccgac
    13141 ccaacgtgga aagttatttt tagcgatgga acacaaatac atctgccggg aattgaataa
    13201 tatatattgc gaggaagagt atgtcaaaat ttgtaataga aggcggcaca aagcttaaag
    13261 gaaaagtaaa tattggggga tttaaaaatg cagctgttgc aattataccg gctacgttac
    13321 tttgtcccga aaggtgcatt attgaaaatg taccaaaaat acaggatgta aatgtatttt
    13381 tggaaatatt gagagaatta ggtgcagaag cggattatat caatgatacg tcaatatatg
    13441 tggacgcatc aaatgtaaaa aattgttatt tggaaaacgg attatcaaaa aaaatgagag
    13501 cctcatatta cttgcttgga gccggattgg ggagatttaa tggagcatcg tgcttgatgc
    13561 ccggcggctg tgatataggc gaaagaccaa ttgaccagca cattaagggg tttgaggcat
    13621 taggaggaaa agttactctt actccggatg gaagaataag tgtacaagca gacagattag
    13681 taggagcaaa aatttatctt gacgttgtaa gcgttggagc aaccataaac attatgttgg
    13741 cagcagtata tgccgaagga aggacaataa ttgaaaatgc ggctaaagag cctcatattg
    13801 ttgatacggc aaacttttta aattcaatgg gtgcagatat taagggcgca ggaacggatg
    13861 taatacgtat aaacggtgta aaggaattaa aaggctgcac ttacaaggtt atacctgacc
    13921 aaattgaagc aggtacatac atgattgctg ctgctgccac aaaaggagat ataataattg
    13981 atgatataat tcccaaacat atggagccta ttaccgcaaa gcttaaagag atgggaatgg
    14041 gcatagaaga gtacggggat tccatgcgtg tttattatga aaaggaattg gttccggtaa
    14101 atatccagac acttccgtat cccggattcc caacagattt gcagcagcca atgactgtgc
    14161 ttttagcttc cttatgcgga gaaagtacag tagttgaagg ggtatcccag gacagattca
    14221 gatacgtgga agagttgaaa aaaatgggcg catgcataga atttacaaag aagcaagcta
    14281 ggattaaagg tacgaaaaat ttaaggggaa cagttgtaca agctacggat ttaagagccg
    14341 gagctgcatt gattatcgca ggtcttattg ctgacggatt gactgaagtg acaaatatac
    14401 agcatataga cagaggatat gaacacattg aaaataaatt tatgaattta ggagccaaaa
    14461 ttaaaagagt agaagattaa taacaggggg aggaaacatt tgaataaagc agtattaatt
    14521 cctgattcat ttaaaggaac attgtcgtcc attgaaatat gcactattat ttctgataaa
    14581 atatataagc attttccgca ctgccatgtg gtatcgattc cggttgctga cggaggcgaa
    14641 gggagtgtgg attgtttttt gtcggcatta ggcggtgaaa aaatagttga aactgtaagc
    14701 tcaccctatt ttgaagatat ggagtcattt tacggcttga ttgatgacac tgcggtaatt
    14761 gaaatggctg catgtgcagg gttacccctg gtggaagaca ggaagaatcc aagccttacg
    14821 acaacttatg gagtggggca gcttataatg tcagcagcaa aaaaaggatg taaaaaaatt
    14881 attgtcggct taggaggttc aagcaccaat gacggtggat gtggtgcagc tgcagccgtt
    14941 ggtgttaaat tttacgataa aaaagggaat gaatttatac caatagggaa aacactaata
    15001 gatatagata gaattgattt gtcccataag gcaaaagaat tggataatat tgaaataata
    15061 acaatgtgtg atatagacaa cccaatgtat ggacctacgg gagctgcata tatattcggt
    15121 cctcaaaaag gagcggatga aaaaatgatt ttggagcttg acaaagggtt aaaaaatctt
    15181 tgtgatgtaa tcgaaagaga aacaggcaaa gatttaaaag acgttccggg cagcggagca
    15241 gcgggagcta tgggtgcggg tatgattgcg ttttttaact ctgtacttca aatgggaatt
    15301 gaaacagtct tagatacggt aaaatttgat gaaataatcg aggacgctga tatgattttt
    15361 accggcgaag gcagaattga ctctcaaagc ttaagaggca aggttgttat aggagtggga
    15421 aaacgagcaa aaaataagaa tattcccgta acagtcattg caggaggagc agcaaataat
    15481 attgatgatg cctatgatat gggagttact tcaatattta caatcaacag actgcctgag
    15541 gactttgaaa taagcaggca taaatccaaa gaaaacttnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnag atatcagctc aatggaaatt gccgaaaatg tagattatga
    15721 tattcttttt gaattaactc ctcttgaaat attttcaggt cagccggcta tagaccatat
    15781 aaaagcggca cttagcagaa aaaaacacgc agttagcgca aacaaggggc ctattgcttg
    15841 ggcatatgaa tcattgcgtg atttagcaga gaaaaacaag gttctttttt accatgaaac
    15901 gactgtaatg gatggcaccc ccttatttaa cttagttgat gaaacactta aattctgtaa
    15961 aataaaagag gtaaaaggaa tattaaattc cactacaaat tttgttttgg aagaaattgc
    16021 aaaaggcaag ccatacaagg aagttataga agaaggaaag aaaaggggat ttgtagagga
    16081 agaccctgca atggatatag aagggtggga tgccgctgcc aagacagccg ccttgctaaa
    16141 tgttctcatg ggagcaaata ttactccttt tgatgttgac agaaaaggga ttgaagatat
    16201 agactacgag caaattaaag aagctgaaaa aagaggaaat atgattaaac ttatttgcta
    16261 cggaggaatt gaagatggta aagtagttgc aagagtaaaa ccggaagaag taaataaaaa
    16321 ttcactcctt gcaactataa caggcacaac atcaattgtc agcatcacaa ctgatttgat
    16381 gggcacaatc tccgtagtgg aacacgaacc tgaaatagaa cagactgctt atggggtgtt
    16441 tagcgattta gtcagggtat taagcaataa aaactgaaaa gccagttaaa attgagggca
    16501 atttcttatc ttaacaagag gtgtataata tggcaaatat aaataatatt ctaaagggca
    16561 ttgaaaagga tatcgaagat atcctatttg catatgtaaa gactgaaagt ttcaccaata
    16621 caataaatga aaaaaaggca gaagaatttt ttctggatta tttttcaaag attccatatt
    16681 ttaaaaatac gccgggctcc tatggtatct atgaaataga aaatgatgca cttaacagga
    16741 atgtaagcta tgcattttta aagggcaggg gtcaggatac gattgtattt attcatcata
    16801 atgatattgt gggtgtggaa gattttaaac tcttaaaaaa atatgcattt tcaccgaaag
    16861 agttaaaggc tcaattgctt agaataaaga atactctcag cgaagatgcg aaaaaggatt
    16921 tggaggatga tacattcata ttcggcaggg gtgtatgcga tatgaagggc ggcggctcaa
    16981 tacaaattgc attgttgaag agatacagcg aattggaaaa cttcaacgga aatattgttt
    17041 tgattgcagt gcctgatgag gaaaatcttt ctgcaggtat gcgttcggca gtaagactat
    17101 tgagtgaatt gcaggataag tataatttaa aatataagct tatgattaat acagagcccc
    17161 atcaaagaaa agatgatgaa acgggagttt tttcggaagg ctccgtggga aaaataatgc
    17221 cttttgtata tgttcgagga tacttggctc atgcgggaaa ggtttttgaa ggattaaatc
    17281 ctattcatat aatgagcaag gttgtaagca ggactgaatt aaacatggat ttcagcgatg
    17341 tggtaggtaa agaggcggca ccgcctccta catggttgta tttaaaagac agcaaggagc
    17401 aatacgacgt atcaatgcca ttaagcgtaa aaggatgttt tggtattttg acattaaacc
    17461 aaacacctca aagcattttg gaaaaagtaa aaaatatatg tgaagaatca tttgatgaag
    17521 taattgatga tatgaataac agttttagaa gatttgcaaa agcaaccggt cttcctttaa
    17581 aatacttgca gtgggaatca aaggttgtaa attttcagga attgttcaaa gaagcagaag
    17641 cagcttacgg tgatgaattt ataagagaat ataataaaga attagaaata ttaggtaaaa
    17701 agttgaaagg aaatgaaata tccataattc actgcaattt tgagttgatt gaatttattt
    17761 acggatatat agatgatata aagccgagaa tagtctatgg acttatacct ccgtattatc
    17821 cgaatgtatc aaatttattt attgatgatt tggatgaaaa tgtcagttct ttatgtgaca
    17881 atttaataaa gtttacacaa gaggaattca atcagaatta taatacggaa tatttttata
    17941 caggaatatc cgatttaagt tattcaagca ttgaaaaaag tggggaaata tataaggcac
    18001 tggaggaatc aatgccgttg ttgggacaat tttataatgt tccggttgaa ctaatagaaa
    18061 agatatccat gccatgcata aatatcggtc catggggcaa ggattttcac aagatgactg
    18121 aaagagtcaa taaagaagat ttatacataa gaactccaag gataattaac aaagcaattt
    18181 ccttaatatt aggcaattaa tattcaccat catataactc tgtgacctcc aaaggaacat
    18241 tatcacggtt aaatccgtct aat
//
LOCUS       DAOSHP010000034        16492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2862_length_16492_cov_8.791203, whole genome shotgun sequence.
ACCESSION   DAOSHP010000034 DAOSHP010000000
VERSION     DAOSHP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_16492_cov_8.791203"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLB33_04930"
     CDS             complement(<1..254)
                     /locus_tag="PLB33_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOG62901.1"
                     /translation="MAKQKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNRRYGTGE
                     FIAFDHIDKAPEERERGITISTSHVEYETPNRHYAHVDCPG"
     gene            complement(289..364)
                     /locus_tag="PLB33_04935"
     tRNA            complement(289..364)
                     /locus_tag="PLB33_04935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(329..331),aa:Thr,seq:ggt)
     gene            complement(515..1165)
                     /locus_tag="PLB33_04940"
     CDS             complement(515..1165)
                     /locus_tag="PLB33_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOG62902.1"
                     /translation="MINSRAIYGNLRYGQEYDLFTDRELIKKFNVGNIEAEKCLMKRY
                     FYLIRRIVNSFNIEDRRKDDILQESMIGLFKAMKTYNKRYDVSFKTYAEICIRRQIIS
                     ALRKSEKYETVNIFTVFNCFESDSEYDFVEQMYDDSYNPENILMYEEEKKYYIEFASQ
                     YLSDYENSVLKEYFNGKTYKEIAEILNTNLKSVDNALQRVKKKTFDKWALFTTKAI"
     gene            complement(1266..1784)
                     /locus_tag="PLB33_04945"
     CDS             complement(1266..1784)
                     /locus_tag="PLB33_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOG62903.1"
                     /translation="MARTKKEYLFIDGYNIINQWQYFRDISRNIENARNKLIELLVEY
                     QAYKGLKVVVVFDAHLVKGSREKKEYVSGVEVVYTKEHETADSYIEKQLDKIGRYEKV
                     RVATSDRAIQQIVLSRGGTRLTAQELILELENTKKDIRIKTENPSQKGANIDRILDTE
                     TLNKLDKIRKKI"
     gene            complement(1787..2515)
                     /gene="rlmB"
                     /locus_tag="PLB33_04950"
     CDS             complement(1787..2515)
                     /gene="rlmB"
                     /locus_tag="PLB33_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOG62904.1"
                     /translation="MRIIEGRNPVIEALRSDAEIDTILINKDAAQGSLNKIFELAKKK
                     NILIKNVDRALLDRLSENKKHQGVIAEAMEYKYKDIDDIFNYAKEKNEKPFIVILDEI
                     TDVHNLGSIIRSAECLGAHGVIIPKRRAAQINSVVAKSSAGAIEYLPVCRVTNISDTL
                     ETLKEKGLWIYGADMEGEKYIYEENFDVPVGLVIGSEGSGLGRLVKEKCDILIKIPMK
                     GNINSLNASNAASIIIYEIMKQRT"
     gene            complement(2512..2907)
                     /locus_tag="PLB33_04955"
     CDS             complement(2512..2907)
                     /locus_tag="PLB33_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOG62905.1"
                     /translation="MNKTYMYSPAQLAYAGDAVYELLVRSYVLKNHDLSVNKMHRLTV
                     RFVKADAQAYIISKLNDELTDEEKKIVKRGRNAKVTSSPKNAEFMDYRYATGFEALLG
                     YLFLNNDIERLMYIFEKVVEIIIEKDDSN"
     gene            complement(2946..4358)
                     /gene="cysS"
                     /locus_tag="PLB33_04960"
     CDS             complement(2946..4358)
                     /gene="cysS"
                     /locus_tag="PLB33_04960"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOG62906.1"
                     /translation="MKIYNTLSRTIEDFKPIDENLVKMYTCGPTVYNYAHLGNLRMYI
                     HEDVFEKTLRYLGYNVKRVMNITDVGHLESDADEGEDKMLKGAKRENKTVWEIAQYYT
                     DAFFDDIDRLNIKRPDVVAKATDYIDEYIEFIKVLEEKGYTYFANGNVYFDITKVKDY
                     TKLSGMDLDSLRSATREGVDLDVNKKNPHDFVLWFTKSKFENQAMKWDSPWGVGYPGW
                     HIECSVISLCNLGEEMDIHCGGVDHIPVHHTNEIAQTESYTGKDWVKYWWHGEFLIDN
                     EGKMSKSSGEFLTLELLIKKGYNPLAYRYFVLNSHYRKQLAFSFDSIASAENAYNKLK
                     SRISSIKNNADGIKENHEAYNKFKNDFENCLRDDLNTANAITVLYEMLKTDTLNNYEK
                     LSLIEDFDKVLSLDLLKEEEKDEVHDELSAYIEEMIQKRQEAKKNKDYQLADQIRAEL
                     LEKGIMLEDTRQGVNWKKIN"
     gene            complement(4600..5808)
                     /locus_tag="PLB33_04965"
     CDS             complement(4600..5808)
                     /locus_tag="PLB33_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOG62907.1"
                     /translation="MFGLVLEGGGARGAYHVGAYKAIKELNIEIGGIAGTSIGAINGA
                     MMVQGDYDLLEKIWLSVDSYELFDLDEKTVEDLRHFNLRNINFSYLLHQSKEILSNRG
                     LDTSRIRELFDYYIDEEKIRSSNIDFGIVTVNLTDKKPMELMKEDIPEGKLIDFLIAS
                     ANLPAFRIEEVDGKKYLDGGFYNNLPIDILANKGYKNIIAVRTLAIGIIRKAKIKDLN
                     IIFIQPVESLGSMLGALDFNRERAEEYINLGYYDAMKVFKKLKGFKYYCIPFEGNFIN
                     ILTDFYNKYKERLCNIGRFLGYEDMCEDRMFFEKIIPRLESILDMKGKNDYQDICIRF
                     FERIAEKYGIERFKIYKAEEFVELTIEKFRENPTAFIKNVPNFIKQNRILSLAVKDDL
                     IVEIFAELFL"
     gene            complement(5818..6252)
                     /locus_tag="PLB33_04970"
     CDS             complement(5818..6252)
                     /locus_tag="PLB33_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOG62908.1"
                     /translation="MKKYLIVNKKILPEVYEKVIEARNLINTGSVKGISEAVKVVGIS
                     RSTYYKYKDYVFSPDENQIGRKAVISMMLKHEKGILSNVLNYLSQENANILTINQSIP
                     INNKASVNVSLDISDLSKSIDEVIADIKKIRGATQVKLLSVE"
     gene            complement(6340..7230)
                     /gene="thrB"
                     /locus_tag="PLB33_04975"
     CDS             complement(6340..7230)
                     /gene="thrB"
                     /locus_tag="PLB33_04975"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOG62909.1"
                     /translation="MVRVTVPATTANIGPGFDVLGLAFKLYNRITFERIEKGLIIKGC
                     PEVYNNNENLVYKSFVVTAENLGKKVDGLKISIDAKIPVSRGLGSSSACIVGGVYGAN
                     TFYKGGLTKDEMFKIAAAIEGHPDNISPCVYGGLTASMIEKERPYAVGYNISSTLKFY
                     ALIPDFETSTSEARKILPKTIDFKDAVFNVSRVAVLLKALETGDMDMINISLDDRLHQ
                     IYRGTLIHEYDNIREICAEAGSPALFISGAGPTLMNITKDEKFPEKIKDSISKLKHKW
                     RIEMLEPDKSGATVEVDSEK"
     gene            complement(7242..8732)
                     /gene="thrC"
                     /locus_tag="PLB33_04980"
     CDS             complement(7242..8732)
                     /gene="thrC"
                     /locus_tag="PLB33_04980"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005968097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG62910.1"
                     /translation="MAKSYESTRNFELKAAASEAVLKGIAEDGGLFVMRNLGERKVNI
                     HSLVGKNYVEIAEVILEILLDDFSKDKLKECIRKAYTDKFSAEEITPVVKVGQNYVTE
                     LFHGPTSAFKDVALSILPHLMTAAYEMNGINSEIIILTATSGDTGKAALEGFKDVKGT
                     RIIVFYPEGGVSQIQETQMITQEGNNTFVCAIEGNFDDAQTGVKKIFANENFIKEMAA
                     KNKILSSANSINIGRLVPQIVYYFVSYMKLVEKGAIKLGDKVNFAVPTGNFGNILAGY
                     YAKILGLPVNKLICASNENNVLYDFIRRGVYDKNRDFHKTISPSMDILVSSNLERLLY
                     YLSGKNNQIVKEYMKKLNTSGIYEISDEMAEKLREDFCGGCVFKEDAQKTIKDTFKKY
                     GYLLDTHTAVAYKALEDYKTETEDDTVSVVLSTASPFKFAKSVYSSLFGETDDDEFVL
                     MEELSEKTKIKIPENLRVLQYKKLRHFEVCSVDEMEDFVISALNEY"
     gene            9170..10333
                     /locus_tag="PLB33_04985"
     CDS             9170..10333
                     /locus_tag="PLB33_04985"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOG62911.1"
                     /translation="MMKYISILGSTGSIGTQTLDVVREHSDKITVCAISGNNNIELLR
                     KQIIEFNPQLCCVMDDSNALKLKKLLPSHIKTEIVSGMEGLIAVAEYEKSEIIVTAVS
                     GMIGLKPTVAAIKKGKSIALANKETLVTGGSYIMNLSKKHKAKIFPVDSEHSAIFQCL
                     MANENKAVNKILLTASGGPFRGKNEEFLKTVTVEDALKHPSWSMGKKITIDSATLMNK
                     GLEVIEAKFLFDIEPEQIEVIVHPQSIIHSGVEFIDHSTIAQLGLPDMRIPIQFALFY
                     PRRMRNNYKSLSLTDIGTLTFEKPDLSTFKCLSLAFEALRAGGTMPAVLNASNEACVR
                     LFLDKRISFLDIGNINEKVMLRHNPVKIDSIETILEAEEFAKQMVINLTKGRG"
     gene            complement(10381..10653)
                     /locus_tag="PLB33_04990"
     CDS             complement(10381..10653)
                     /locus_tag="PLB33_04990"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOG62912.1"
                     /translation="MTNYWDIAEKDVVNIKNGEVMGRFDDVEIDTRKGKITSFYIEEG
                     SKFMGMLGKTKPRRIRWEEILKIGADVIIVNVDDDIHNKAIKNMLD"
     gene            complement(10753..11139)
                     /locus_tag="PLB33_04995"
     CDS             complement(10753..11139)
                     /locus_tag="PLB33_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62913.1"
                     /translation="MIIKRYSDVDESPLFDMLKEEGDDWSDYHGEKGRDKYIKALKSS
                     LTYVIYDDNILCGFVRCREDDGFGVYVYDLLVRKSYRGRQLGKKLMEQVCKDFANQPV
                     YVMSDVDPYYEKLGYRREGSIFEVKI"
     gene            complement(11406..12182)
                     /gene="sigG"
                     /locus_tag="PLB33_05000"
     CDS             complement(11406..12182)
                     /gene="sigG"
                     /locus_tag="PLB33_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOG62914.1"
                     /translation="MNKVVICGVNTSQLPTIKTSEMRGMIKKIHEGDTELREKFIQGN
                     LRLVLSVIQRFNSRNEPVDDLFQIGCVGLIKAIDNFDLSLDVKFSTYAVPMIIGEIRR
                     YLRDNNSIRVSRSMKDTAYKALQVRERLTAENSQEPTIAEIAKEVGVDKEEIIFALES
                     IQEPVSLFEPIYNDGGDALYVVDQVRDEKNIDEKWIEKITLEDSINNLSEREKQIVDM
                     RFYKGKTQMEVADEIGISQAQVSRLEKSALNQLKKGFKTS"
     gene            complement(12246..12968)
                     /gene="sigE"
                     /locus_tag="PLB33_05005"
     CDS             complement(12246..12968)
                     /gene="sigE"
                     /locus_tag="PLB33_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HOG62915.1"
                     /translation="MNMLMHVKTFFKKIINFIIDKLGIIKDIFYIGGSDTLPPPLTQE
                     EEKEIISRLKDSNEAKTILIEHNLRLVVYLAKKFESTGINVEDLISIGTIGLIKAVNT
                     FKAEKNIKLATYASKCIENEILMHLRRVGKTKTEISLDEPLNIDWDGNELLLSDILGT
                     EEDVVFRNMENEVDLSLLNDSIQKLNKREYMIMKLRFGLNNTKSYTQKEVADMLGISQ
                     SYISRLEKKILNRLKKDINKAV"
     gene            complement(12946..13827)
                     /locus_tag="PLB33_05010"
     CDS             complement(12946..13827)
                     /locus_tag="PLB33_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOG62916.1"
                     /translation="MVYYVEYIFVENFIIDFILLFITGKLVKRAIIFKRLIAASLIGS
                     LYVIVTLYAGKEFMNNFIVKFSISVLMIMVAYDSKGILTNIRVIICFYITSLIMVGII
                     SALYILTYDKLTVNAIIMSIFTGYAALHFFFKEIKDRIVKHNYKRKVNVSICGKSKTF
                     RGYIDTGNELTDPITGKPVMVVNIDCIKEILGDELCNKIMEFYENKSYEKLIDENNSI
                     NLRVIKYKTISNSGENMVCIVPDEIEIINNNKSILVDAIIGIHPKRISMDDYDALLFK
                     KLLDWEVETENEYVDAC"
     gene            complement(14317..>16492)
                     /locus_tag="PLB33_05015"
     CDS             complement(14317..>16492)
                     /locus_tag="PLB33_05015"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62917.1"
                     /translation="WDPNTAVYAERYYPIHAGDDAAAKMTDIYEGIAGTINALANGYV
                     TDVIHKNFELTDDSKEYLEDQGVVITENDDGTTTLVFNNVKAYGEPNPLPVFEVKVKD
                     GVYGTGYTNEEAYYTFTLFGDEVSKTKNFEQPIVAIKPTAANDDGYETFQDQVLTVLL
                     AQSILKNDETVKIEDGDYSVSNLVVKDEYVTTINTNKGGTAVVAADGTFVYTPLEGFT
                     GDDTFVYKNKATVTIDGVDVFGLAGDYISNEATVTITVKPITGQTVQYTIRHLVQESN
                     EVLDSENGEGEEGQVITGYAKTFEGYILAPEEEDVKQITLEIESNNTITFWYVPDPIN
                     QVSYTVRYLDNVTEEPVAPEKTGYGNPEQEITEYAIDVPGYTPLKEEETFVLDDENII
                     ITFYYEKDFEGNVIIIKHYRQTGSNARNLVRTDAPIPVTTGGTIYGEDYKDSSLEVSR
                     YKFKESTPASLDVVAEVPTSGAIQYVFELLYYRDSSGGGGGGGGGTVIEDPEIPLAEL
                     EKFDHFAYVIGYPEGDIRPMNNITREEVAMIFYRLLTDDSRNQLLSNTNPFTDVESGR
                     WSNRAISTLYNAGIISGYPDGTFRPSDSISRAEFATIAAKFDDLDLGSASKFTDIFGH
                     WAEKYITSAENKGWINGYPDMTFKPEQDITRAEAMTLINNVLERAVPAENIHSDAMFW
                     PDIDEDDWYFEAIMEATNSHDYVIEEDGDELWTGMKPNKVWP"
BASE COUNT         4802 a         3476 c         2354 g         5860 t
ORIGIN      
        1 cccgggcagt caacgtgtgc gtagtgtctg tttggagttt catattcaac gtgagatgtt
       61 gatattgtaa ttcctctttc cctttcttcc ggagctttat caatatgatc aaatgctata
      121 aattctccgg ttccgtatct tctgttcaat actaacgtaa ttgccgccgt taatgttgtt
      181 tttccgtggt caacgtgacc gatagtacct atattaacat gcggtttcgt tctttcaaat
      241 ttttgctttg ccatttcaat ccctcccgga ttatttttta aatttttttg gagcttacga
      301 gcagactcga actgccgacc tcctcattac caatgaggtg ctctacctcc tgagctacgc
      361 aagcatgctg tcaaaccctt gttgttaaca agtttcatta tatcaacaac cggaaaataa
      421 atcaatagga aaatttaaga gattctcctc accccacccg atgactttgt cgttttgagt
      481 gcaggctgcg aagaatctca tattatgtat tgtgtcatat tgcttttgtt gtaaacaatg
      541 cccatttatc gaatgttttt ttcttaaccc tttgcaatgc attatctact gattttaaat
      601 tagtatttaa tatctcagca atttctttat atgtctttcc gttaaaatat tcctttaata
      661 cggagttttc ataatcgctt aaatattgtg atgcaaattc aatatagtat tttttttctt
      721 cctcatacat caatatgttt tcaggattat aagaatcatc atacatttgc tctacaaaat
      781 catattcact gtcactttca aaacaattaa atacagtaaa tatattaact gtttcatatt
      841 tctctgactt tcgcagtgct gaaataattt gtcttcttat gcatatttca gcatatgttt
      901 taaagcttac atcatacctc ttattataag ttttcatcgc tttaaaaagc ccaatcattg
      961 attcctgcaa aatatcatct tttcttctgt cttctatatt aaaagagttg acaattcttc
     1021 taatcaaata aaaatatctt ttcattaagc atttttcagc ttctatattt cctacattga
     1081 atttttttat gagttctcta tccgtaaata agtcatattc ttgcccatat cttagattac
     1141 cgtatatagc gcgagaatta atcataaagt tcctccaatt cataacatat aaaataataa
     1201 taaattatat actgttataa tcaaaggata tacatattga gaaatcatga attgttgtca
     1261 aagtgctaaa ttttttttct aatcttatca agtttattca aagtttcggt atccagtatc
     1321 ctatcgatat tagctccttt ttgactcggg ttttcggttt ttatcctaat atccttttta
     1381 gtgttttcca attccaaaat cagctcttga gcagtgagcc ttgtccctcc tcttgacaaa
     1441 acaatttgtt gaattgctct gtctgacgtg gcaacccgga ctttttcata ccttcctatc
     1501 ttatcaagct gcttttcaat atagctgtct gctgtttcat gctccttagt atagacaact
     1561 tccactcctg aaacatactc tttcttttcg cggctgcctt ttaccaagtg agcatcaaat
     1621 acaacaacta ccttcagtcc tttgtatgct tgatattcaa ctagaagctc aataagctta
     1681 ttccttgcgt tttcgatatt tcttgaaata tccctgaaat actgccactg atttataata
     1741 ttgtatccat ctataaaaag atattccttc tttgttctag ccatttttat gtcctttgct
     1801 tcattatttc atatataata attgaagctg cattggaagc atttaaagaa tttatatttc
     1861 ccttcatggg gattttaatc aaaatatcac atttttcttt aaccagtctt cctaagcctg
     1921 aaccctcgct tcctataacc aaccctaccg gaacatcaaa attctcttca tagatgtatt
     1981 tttcaccttc catgtctgct ccgtatatcc aaagtccctt ttcctttaat gtttccaatg
     2041 tgtcacttat attggtaacc ctgcagacag gtagatattc aattgcaccg gcggaagatt
     2101 ttgcaacaac actatttatt tgagcagctc ttcttttggg aattataaca ccgtgggcgc
     2161 ctaagcattc ggccgagcga ataatagagc ctaaattatg gacatcggta atttcatcaa
     2221 gaatcactat aaaaggcttt tcgttttttt cttttgcata attaaatata tcgtcaatat
     2281 ccttatattt gtattccata gcttctgcta tgactccctg atgctttttg ttctcgctta
     2341 atctgtccaa aagtgcccta tcaacatttt taatcaatat attctttttt ttagccagct
     2401 cgaaaatttt atttaaagag ccttgagccg catccttatt tatcagtatg gtatcaattt
     2461 cggcatcgct tctcaatgct tcaattaccg gattccttcc ttcgattatc ctcaattact
     2521 gtcatccttt tctattatta tctcaacaac tttttcaaat atatacataa gtctttcaat
     2581 atcattattt aaaaacagat acccaagcaa agcttcaaat cccgtagcat atctgtaatc
     2641 cataaattca gcatttttag gagaagaagt aaccttggca tttcttcctc tctttacgat
     2701 tttcttctct tcatcggtca gttcatcatt taatttgctt ataatataag cctgagcatc
     2761 agcctttaca aacctgacgg taagcctgtg cattttattt acgcttaagt catgattttt
     2821 taaaacatag cttcttacca gcaattcata aacagcatca cctgcatatg ccagttgtgc
     2881 aggtgaatac atatatgttt tgttcatttt atcgcttatt ttattaagaa cttcaaattc
     2941 tttggttaat ttattttctt ccaatttaca ccctgcctgg tatcttcaag cataataccc
     3001 ttttccaaga gttctgccct gatttggtca gccagctgat agtctttatt tttctttgct
     3061 tcctgccttt tttgaatcat ttcctcgata tatgcagaca gctcatcatg aacctcatct
     3121 ttttcttctt cttttaacaa atccaatgac aagaccttat caaaatcttc tataaggctt
     3181 aacttttcat aattgtttaa tgtatccgtt ttaagcattt catataaaac ggttattgca
     3241 tttgcggtat ttaaatcatc ccttaaacaa ttttcaaaat catttttgaa tttattataa
     3301 gcttcatgat tttcttttat tccatccgca ttatttttaa tggagctgat tctgcttttc
     3361 agcttattat atgcattttc agcagaagca atagaatcaa acgaaaaagc aagctgcttt
     3421 ctgtaatggg aatttaaaac aaaatacctg tatgccaaag gattgtaacc tttcttaata
     3481 aggagttcaa gggtcaaaaa ttcaccgctt gacttactca tctttccttc attgtcgatg
     3541 aggaattcac cgtgccacca gtatttaacc caatccttgc cggtatagct ctctgtttga
     3601 gctatttcat tggtgtgatg aaccggaata tggtctaccc ctccgcagtg aatatccatt
     3661 tcttccccta aattgcagag gctgataacg gaacactcta tatgccatcc gggataacct
     3721 actccccatg gagaatccca tttcattgcc tgattttcaa atttggattt tgtaaaccac
     3781 aaaacaaagt catgagggtt tttcttgtta acatctaagt caaccccttc ccttgtggcg
     3841 cttcttaaac tgtccaaatc cattccggaa agctttgtat aatccttaac cttggttatg
     3901 tcgaaataca cattgccgtt tgcgaaatat gtgtagccct tttcttccaa aactttgata
     3961 aactcaatat attcgtctat gtagtcagta gcttttgcaa caacatcagg tcttttaata
     4021 ttaagtctgt ctatatcgtc aaaaaaggca tcagtataat attgtgcaat ttcccaaacg
     4081 gttttatttt ctctttttgc acccttcagc attttgtctt cgccttcatc ggcatctgat
     4141 tccaagtgac ctacatcagt aatgttcatt acccttttaa cattgtaacc taagtatctg
     4201 agggttttct caaaaacatc ctcgtgaatg tacattctta aattgcccaa atgtgcatag
     4261 ttgtatacgg taggtccgca agtgtacatt tttactaagt tttcatcaat tggtttaaaa
     4321 tcttctattg ttctggataa tgtgttatag attttcatgt aaatccgctc ctcttcactt
     4381 aaacttatta tatattacct ttgaaacagt atcaagcata atttgccaat attttttgtt
     4441 aagataaagt aactttaatg caaaaattgt cgaaaaatag cacaatgaca aggggacggg
     4501 ggtactgtca tcttttgtga cagcaccccc gttaaccgtt gatgcgacgg tgccaggcac
     4561 cttcatgagg tgcctggcac caagtgtttc cccataccac taaaggaata actcagcaaa
     4621 aatttccaca atcaaatcat ccttaactgc taatgataaa attctgtttt gcttaataaa
     4681 attcggtaca tttttaataa aggcagtagg attttctctg aatttttcga ttgtcaattc
     4741 aacaaattct tcagccttat atattttaaa cctttcaatg ccatattttt cggcaattct
     4801 ttcaaaaaac cgaatgcata tgtcctggta gtcatttttg cccttcatat ctaatattga
     4861 ttctaatctg ggtataattt tttcaaaaaa cattctgtct tcgcacatat cttcatagcc
     4921 caaaaaacgt ccgatattac ataatctttc tttgtattta ttatagaaat ctgtcaatat
     4981 atttataaaa ttaccctcaa aaggaataca gtaatattta aagcctttta gctttttaaa
     5041 aaccttcatg gcgtcataat agcctaaatt aatgtattcc tcagctcttt ccctgttaaa
     5101 atctaaagcg ccgagcattg agcctaagct ttcaaccggt tgaataaaga taatatttaa
     5161 gtcttttatt tttgcctttc tgataatacc gattgccagt gttcttacgg caattatatt
     5221 cttatatcct ttgttggcta gaatatctat gggaagattg ttataaaaac ctccgtctaa
     5281 atatttcttc ccatccactt cctctattct aaaagcagga aggtttgcgc ttgctattaa
     5341 aaaatcaatt aatttcccct ccggtatatc ctccttcata agctccatcg gcttcttgtc
     5401 ggtcagatta acagtaacta ttccaaaatc aatattagaa ctgcggattt tttcctcatc
     5461 aatataatag tcaaacaatt ctcttattct tgaagtgtcc aaccctctat tgcttagtat
     5521 ttctttagat tgatgcaaaa gataggaaaa attaatattt cgcaaattaa agtgcctcaa
     5581 atcttcaact gtcttctcgt caagatcaaa caattcatag gaatcaacgc ttaaccatat
     5641 cttttccagc aaatcatagt ccccttggac catcattgcc ccattaatag cccctatgga
     5701 tgttccggcg attcctccga tttctatatt taattctttt atagctttat aggctcctac
     5761 atggtaggct ccccttgcgc ctcctccttc taaaaccaat ccgaacataa tatcacccta
     5821 ttcaactgat aataatttta cttgagttgc gccccttatt ttttttatat cggctattac
     5881 ttcgtcaata gacttgctta aatctgaaat atccaatgat acatttacag atgccttgtt
     5941 gttaattgga atgctttgat ttatggtaag aatatttgca ttttcctgag acaaataatt
     6001 caaaacattt gagagtattc ccttttcatg ctttagcatc atcgaaatta ctgcttttct
     6061 gcctatttga ttttcgtccg gagaaaaaac ataatccttg tacttataat atgtactcct
     6121 gcttatacca acaaccttaa ctgcctcgct gatacctttc acacttccgg tgttgattaa
     6181 atttcttgct tcaattacct tttcatatac ttccggaaga atttttttat tgacaatcaa
     6241 atactttttc attatatcca cctccattca ttattgatta ttgcgcagtt actattttaa
     6301 tgtcattctg agagtcttag ctcgaaagat gccctaaccc tatttttcag agtcgacttc
     6361 tactgttgct ccgcttttat cgggttccag catttcaatt ctccatttat gttttaattt
     6421 gctaatgctg tctttaattt tttccggaaa tttctcatct tttgtaatat tcatcaaagt
     6481 cggtcctgcg ccgcttataa acaatgccgg cgagccagct tcagcacaaa tttctcttat
     6541 attatcgtat tcatgaatta aagtgcctct gtaaatctga tgcaatctat cgtccaatga
     6601 aatattaatc atatccatat cgcctgtttc caatgccttt agtaatactg caacccttga
     6661 tacattaaat accgcatcct tgaagtcaat tgttttaggg agaatttttc ttgcttcact
     6721 tgtcgaagtt tcaaaatcag gaattaaggc ataaaacttt agtgtactgc ttatattgta
     6781 tccgacggcg taaggtcttt ctttttcaat catggatgct gtcagtcctc cataaacaca
     6841 gggagaaata ttatcgggat gtccttcaat cgcagcggca attttaaaca tttcatcttt
     6901 tgtcaatcct cccttgtaaa aagtgttagc accataaacc cctcccacaa tacatgccga
     6961 gctgcttcct aaaccccttg aaacaggaat tttggcatct atggaaattt taagtccatc
     7021 aacctttttc cctaaatttt cagcagtaac aacaaacgat ttataaacta aattttcatt
     7081 attattataa acttccgggc aacctttaat tatcagtccc ttttcaattc tctcaaaagt
     7141 aattctgtta tacagtttaa atgcaagtcc caagacatca aagccgggtc ctatattggc
     7201 agtagttgcc ggaacagtta ctctaaccat ttttcacctc attaatattc atttaaagca
     7261 gaaatcacaa agtcttccat ttcatcaaca ctgcaaacct caaaatgcct taatttttta
     7321 tattgaagta ctcttaggtt ttcaggtatt tttattttag ttttttcgga caattcttcc
     7381 attaatacaa attcatcatc atccgtctct ccaaaaagtg agctgtaaac acttttggca
     7441 aatttaaatg ggcttgcagt tgaaagaact acgcttacag tatcatcctc agtttctgtt
     7501 ttataatcct caagcgcctt atatgccacc gctgtatggg tatcaaggag atatccgtat
     7561 tttttaaacg tatccttgat ggttttttga gcatcttcct taaaaacaca tcctccgcag
     7621 aaatcctctc tcagtttttc agccatttca tcggatattt cataaatacc tgaagtattt
     7681 agctttttca tgtattcttt gactatttga ttgtttttgc cgctcaaata atacaagagc
     7741 ctttcaagat tgcttgatac caaaatatcc atggatggtg aaatggtttt atgaaaatct
     7801 ctgtttttgt cataaactcc ccttctgata aaatcataca gcacattgtt ttcatttgaa
     7861 gcacaaataa gcttatttac gggaagtccc aatatctttg cataatatcc tgccaaaata
     7921 tttccaaaat ttccggtagg tactgcaaaa ttaaccttgt ctcctagttt gatagcaccc
     7981 ttttcaacca acttcatata ggaaacgaaa taataaacta tctgaggaac caatcttcct
     8041 atattgatgg aatttgcaga tgataatatt ttattttttg cagccatttc cttaatgaaa
     8101 ttttcattag caaaaatttt cttaaccccg gtctgagcat catcaaaatt tccttcaatt
     8161 gcacaaacaa atgtattatt cccctcctga gttatcattt gtgtttcttg aatctgactg
     8221 accccgcctt cgggataaaa tacaataatt ctcgttcctt taacatcctt aaatccttcc
     8281 aatgcagctt tacccgtatc ccctgaagtt gcggtaagta ttattatctc gctgtttatg
     8341 ccgttcattt cataagcagc cgtcattaga tgcggtaata ttgaaagagc cacatcctta
     8401 aatgccgaag taggtccatg gaaaagctct gtcacataat tttgtcccac ctttacaaca
     8461 ggagttattt cttctgcgct gaatttatcc gtatacgctt ttcttataca ttccttaagt
     8521 ttatcctttg aaaaatcatc gagcaaaatt tcgagaataa cttcagcaat ttccacataa
     8581 tttttaccga ctagactatg tatatttact tttctttcac ctaaatttct cattacgaat
     8641 aatccgccat cctctgcaat accctttaat acggcttccg aggcagcagc tttcaattca
     8701 aaatttctgg tgctttcata actctttgcc atattccctc cgtcctcata tataataata
     8761 tatgatatac tgttcccgat ataattacaa gtgttttttt aaatatgaca atgagaagtc
     8821 cttgatgtca tcctgagcgc aagcgaagga tctaccccat ttgtcatcct gagcgcaagc
     8881 gaaggatcta ccccatttgt catcctgagc gcaagcgaag gatctcaggt tttataaaga
     8941 tgagattctt cgagctaaag ctctcagaat gacacgtagt atattaaaag caatgacact
     9001 ccacaagtta ttcatagcaa atgtcaattt caataagaaa ataattagtt tttcgttagt
     9061 ttcagacaaa ggagatgtca taaaatatta ctgttttttt ttaaatacta tggtagaata
     9121 gcatatgaag aaaacgcact tttattacaa cttcaaaata taaggagcaa ttatgaaata
     9181 tatcagtatt ttaggttcca ccggttcaat cggaacacaa accttagatg ttgtacgtga
     9241 acattcagat aaaattacag tttgtgcaat ttccggaaat aacaatattg agcttttaag
     9301 aaaacaaatt attgaattta atccacaact ctgctgtgtg atggatgaca gtaatgcgct
     9361 gaaattaaaa aagttgctgc catctcatat aaaaacggaa attgtttcag gcatggaagg
     9421 cttaattgca gtagcagaat atgagaaaag cgaaattatt gttacggcag tatccggaat
     9481 gatcggtctt aagcctacag ttgctgcaat aaaaaaagga aaatcaatag cccttgccaa
     9541 taaggaaaca ttggtaacag gcggaagtta tataatgaat ttatcaaaaa aacacaaagc
     9601 taaaattttc cccgttgaca gtgagcacag cgctattttc caatgcctga tggcaaatga
     9661 aaacaaagct gtcaataaaa tattgctgac tgcttcaggc ggtcctttca gaggcaaaaa
     9721 tgaagaattc ctaaaaactg ttaccgtcga agatgccctg aaacatccca gttggtcaat
     9781 gggcaaaaaa ataaccatag attccgccac attaatgaac aaaggtttgg aagtaatcga
     9841 agcaaaattc ttgtttgaca ttgagccgga gcaaatcgaa gttattgtgc accctcaaag
     9901 cattatacat tcgggagttg aatttatcga ccactcaaca attgcacagc tgggacttcc
     9961 ggatatgcga atccccatac aatttgcgct gttttatcca agacgtatga gaaacaatta
    10021 taaaagcctg tccttaacag acataggtac cttaacattt gaaaagccgg atttatctac
    10081 attcaaatgc ctgtctttag cctttgaagc gctaagggca ggcggcacca tgcctgccgt
    10141 actcaatgca tccaacgaag cctgtgtcag actgtttctg gataaaagaa taagtttcct
    10201 tgatatagga aacataaatg aaaaagtaat gctgagacac aaccctgtaa aaatagattc
    10261 aatagaaaca atactcgaag cagaagagtt tgccaaacaa atggtaatca atttgacaaa
    10321 aggacggggt taatgtcgta taacatagcc cccgtcccct tgtcattgtc cccttgtcgt
    10381 ctagtccaac atatttttta ttgctttgtt atgtatatca tcatcaacgt taacaattat
    10441 tacatccgcg ccgattttta gtatttcttc ccatctgatt cttctgggtt ttgtcttgcc
    10501 gagcatcccc ataaacttac ttccttcctc tatataaaag gatgtaattt tgccctttct
    10561 tgtatctatt tctacatcgt caaacctgcc cataacctcg ccgtttttta tattaacaac
    10621 atccttttca gctatgtccc aataattagt caaaacatca ccaccttacc cttttataat
    10681 aaaatatgta caacagttat caaatatgac aaacattgta gcaaatcacc ataagggtat
    10741 acagcaagtt acttatattt tgacttcaaa aatcgaacct tctcttcggt aaccaagctt
    10801 ttcataataa gggtctacat cgctcatcac ataaacgggt tgatttgcaa aatccttgca
    10861 cacttgttcc ataagctttt taccaagctg tctcccacga taggattttc tcacaagtaa
    10921 atcatagaca tagacaccaa aaccatcatc ctcacgacat cgaacaaagc cgcacaggat
    10981 attatcgtca taaattacat aagtaaggct tgatttcaat gctttaatat atttatcacg
    11041 gcctttttct ccatgatagt cgctccaatc gtccccctct tcttttagca tatcaaacaa
    11101 gggtgactca tcaacatcag aatatctttt tataatcatt tctttctcct ttaatctcac
    11161 caagacctga ttgttgtctg ctgcatgatg acaacaacat tcataatttc tccgtgaaaa
    11221 ttctatataa tatttggcga ggaatatccc cttttgcgaa agtattgtta ttatcgttta
    11281 actaatttta ctttataata taattagaat caacaaattc tttatatgtg taatatctcc
    11341 atcccatgac acaaaaaagg aacgctgtac gttcctcaaa tttacaaata cgagtttatg
    11401 gcatattatg aagttttgaa tcccttcttc aactggttca gtgctgattt ttcaaggcgg
    11461 gatacttgcg cttgggaaat tccgatttca tccgccacct ccatttgcgt cttcccttta
    11521 taaaaacgca tatcaacaat ttgcttttcc ctttcggata agttgtttat actatcttcc
    11581 aaagttattt tttcaatcca tttttcatct atattctttt cgtcccttac ctggtctaca
    11641 acataaagag catctccccc gtcattgtaa ataggttcaa acaacgatac gggctcttgt
    11701 atagactcca aggcaaatat gatttcttcc ttatcaactc ccacctcttt tgcaatttca
    11761 gcaattgtgg gctcctgtga attttcagcc gttaatcttt cccttacctg caatgctttg
    11821 tatgcagtat ctttcattga cctcgatact cttatggaat tattgtctct aagatatctt
    11881 ctgatttccc ctattatcat ggggacagca taggtagaaa actttacatc taaagacaag
    11941 tcaaaattat caatagcctt tatcagtccg acgcaaccaa tttgaaacaa atcgtcaaca
    12001 ggctcatttc ttgaattaaa tctttgaatt acgctgagta ctagtcttaa attcccttga
    12061 atgaacttct ccctaagctc agtatcacct tcatggattt ttttaatcat acctctcatt
    12121 tccgatgtct ttatggtggg cagctgagaa gtattgacgc cgcatataac tactttgttc
    12181 ataatcgtcg attccccttt aaagaaatta tcccccgtta aaattatttc ctcggctgta
    12241 tctttttata cagctttgtt aatatccttt tttagtctgt ttagtatctt tttttccagc
    12301 cgtgaaatgt aagactgtga tattccaagc atatcagcca cttccttctg cgtgtagctc
    12361 ttggtgttgt taaggccgaa ccttaatttc ataatcatat attctctttt atttaatttt
    12421 tgaattgaat cattaagaag acttaaatcc acttcatttt ccatgttacg aaatactaca
    12481 tcttcttccg tacctaaaat atctgacagc aaaagctcat ttccatccca atcaatattc
    12541 aaaggctcat ctaaagaaat ttccgtcttg gtttttccta cccgtcttaa atgcattaat
    12601 atttcatttt caatacactt agaagcataa gttgcaagct taatattttt ttctgcctta
    12661 aatgtattca cagcttttat tagtccgata gttcctatgg aaatcaaatc ctccacattt
    12721 attccggtag actcaaactt ctttgcaagg tatacaacaa gccttaaatt atgctcaatt
    12781 aaaattgttt ttgcctcgtt agaatctttt agtctgctta ttatttcttt ttcttcctcc
    12841 tgtgtcaagg ggggaggtaa tgtatcgctt cctccgatat aaaaaatatc cttaattatt
    12901 cctaatttat ctattataaa gttaattatt tttttaaaga aagttttaac atgcatcaac
    12961 atattcattt tccgtctcca cctcccaatc aagtaatttt ttgaacaaaa gcgcatcgta
    13021 atcatccatg ctgattcttt tcggatgtat tcctataatt gcatcaacca aaatactttt
    13081 attattattt attatttcta tttcatccgg cactatgcaa accatgtttt caccgctgtt
    13141 gcttatagtt ttgtatttaa taactcttaa attaatgctg ttgttttcat caattaattt
    13201 ttcatagctt ttattttcgt aaaattccat aattttgttg catagctcat ctcccaaaat
    13261 ttcttttatg caatcaatat ttacaaccat aacaggtttt ccggtaatcg gatctgtcaa
    13321 ttcattgccc gtatcaatgt accctctgaa tgtcttggat ttaccgcata tgctgacatt
    13381 cacctttctt ttgtaattat gcttaacaat tctgtcttta atttctttaa aaaagaaatg
    13441 caatgcagca taccctgtaa atatggacat gataattgca ttcacggtta atttatcata
    13501 tgttagaatg tataaggcgg aaataattcc caccattatc aatgaggtaa tgtaaaaaca
    13561 aattattact ctgatattag tcaatatccc tttggaatca tatgctacca ttatcattaa
    13621 tacggaaata ctgaatttta cgatgaaatt gttcataaat tcctttcccg catataaagt
    13681 aacaataaca tacaatgagc cgattaaaga tgcagcaata agtcttttga atattattgc
    13741 cctttttaca agctttccgg taataaaaag caaaataaaa tctatgataa aattttcgac
    13801 aaaaatatat tcaacataat aaaccaagcc ttccaactcc ctcgagattc ttcatctgcg
    13861 gttcagaatg attaatttgt catcctgagt cttttcctga agcatctcac ccatcgtccc
    13921 ttgtttctat agccaagcaa tctattaaca attataaatg accttatcga aatattttgt
    13981 cataacatgt aatcaaaaat aaaaaaatac gctttttatg aatactatag cgtatttcgt
    14041 atagttcgcc gcaaaaatta tgctttacat ttttctgctt tttttgaacc cttctacaat
    14101 atacttaata agtttatgtt gtacacaacc actttatgtc atccatagcg taggggcctt
    14161 tgagattctt cgagctgaag ctctcagaat gacaaaaaga gaaagttgtc atacctagct
    14221 taggcaaaac attagatttt gtatcctttg tcataaacaa gcggggcatt tagccccgct
    14281 attatttaaa tgttattact ttttttggat attcctttac ggccatacct tgttcggttt
    14341 cattccggtc caaagctcat cgccgtcttc ctcgataaca taatcgtgac tgttggttgc
    14401 ttccattata gcctcaaaat accagtcatc ttcatcaata tcaggccaga acattgcatc
    14461 ggaatgtatg ttttctgcag gaacagctct ttcaagaaca ttgttaatca atgtcattgc
    14521 ttcagctctc gtgatatcct gctcaggttt aaatgtcatg tccggataac cattaatcca
    14581 tcccttgttt tcagcagaag taatatattt ttctgcccaa tgtccgaata tatcagtgaa
    14641 tttgcttgca cttcctaagt ccaaatcatc gaatttagct gcaatagttg caaattctgc
    14701 tctcgaaatc gagtctgacg gtctgaatgt tccgtccggg taaccgctga ttatacctgc
    14761 attgtagagt gtagatattg cacggtttga ccacctgccg gattcaacat ccgtaaacgg
    14821 attcgtattg cttaataact gatttctgct gtcgtcagtc aacaacctgt agaaaatcat
    14881 cgccacttct tctcttgtta tattgttcat aggtcttatg tcgccttcag gataaccgat
    14941 tacatatgcg aagtggtcaa atttttctaa ctcagccaac ggaatttccg gatcttcgat
    15001 gacggtgcct ccaccaccgc cgccgccacc gccgctgctg tctctgtaat acagaagttc
    15061 aaatacatat tggatagcac cacttgtcgg tacttcagca actacatcaa gagatgccgg
    15121 ggttgattcc ttaaatttat acctgctaac ttcaagcgag ctgtccttat aatcctcacc
    15181 atagattgtt ccgcctgttg taaccggaat cggtgcatca gttctcacca gattacgtgc
    15241 attgctgcct gtctgcctat aatgctttat gataattaca tttccttcaa aatctttttc
    15301 ataatagaaa gtaattatta tattctcatc atcaagaaca aatgtttcct cttcttttaa
    15361 aggtgtatat ccgggtacat ctattgcata ttcggttatt tcttgttccg gatttccata
    15421 gccggttttt tccggtgcaa ccggttcttc ggtcacatta tcaaggtatc gaactgtata
    15481 agatacttgg tttatcggat caggaacata ccagaaagta attgtattgt tgctttcaat
    15541 ttcaagagta atttgtttta catcttcctc ttccggtgca aggatataac cttcaaaggt
    15601 ctttgcatat cctgtaatta cctgtccttc ttctccctca ccattttccg agtcaagaac
    15661 ttcatttgat tcctgtacaa gatgtctaat cgtgtattgt acggtctgtc ccgtaatcgg
    15721 cttaacagta attgttacgg ttgcttcatt ggaaatataa tcaccggcaa gaccgaatac
    15781 atccacacca tcaattgtta ctgttgcttt gtttttgtat acaaatgtgt catctcctgt
    15841 aaatccttcc agcggagtat atacaaaagt tccgtcggca gctactactg cagtaccgcc
    15901 cttattagta tttatagttg ttacatattc atctttaaca actaaattac taaccgaata
    15961 atcaccatct tctattttaa cggtttcatc gtttttaagg atactttgtg caagcagaac
    16021 cgttaatact tggtcttgga atgtttcata tccgtcgtca ttagctgccg taggtttaat
    16081 tgcaactata ggttgttcga aattctttgt cttagatact tcatctccaa aaagagtgaa
    16141 tgtataatat gcctcttcat ttgtatagcc tgttccgtaa acaccgtctt taacttttac
    16201 ttcaaagaca ggcagcggat taggttctcc atatgccttt acattattga aaaccaatgt
    16261 agtggttcca tcatcatttt ctgttattac aactccctga tcttcaagat attcttttga
    16321 atcatcagtc agttcgaaat ttttatgtat aacatcagta acatatccgt ttgccaaagc
    16381 atttattgtg ccggcaatac cttcataaat gtcagtcatc ttagcagcag catcgtctcc
    16441 tgcatgaatc ggataatatc tttcggcata tactgctgta ttggggtccc ac
//
LOCUS       DAOSHP010000035        16279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2923_length_16279_cov_8.776381, whole genome shotgun sequence.
ACCESSION   DAOSHP010000035 DAOSHP010000000
VERSION     DAOSHP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16279
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2923_length_16279_cov_8.776381"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1397
                     /locus_tag="PLB33_05020"
     CDS             171..1397
                     /locus_tag="PLB33_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOG62918.1"
                     /translation="MINIKRKIIISVVFALLVILSAGCQQNDKDVGALNDTKIIRAEE
                     WKNQHPDIYASFMTNAEMKETKYGGSVPDDYLEEYPNLKVFYDGYGFSKEYLGARGHI
                     YALEDVINTSRPKGGASCLACKAADFNVQLANEGEGVNKIPFNEFVEANPEMEGISCY
                     DCHRNTPGEVNITRTHLTQGLTFVEEEKSGNLACAQCHVEYYLAPDTNKVILPWHNGI
                     DTDNMIEYYDEINFRDYEHPTTGAQILKAQHPEFETFKGSIHDAQGLTCIDCHMPRIA
                     GEENLKSHQWTSPLKSKEGLTKSCIPCHGGEPEDIISKVEGVQESVYKKTNEVSDELL
                     TFINRLGKAVEDGDLPDEVLEQLRGIHRNSQFMWDFVFVENSEGFHNSQKTHKNLDNA
                     RELITEGIEILEEHGK"
     gene            complement(1447..2037)
                     /locus_tag="PLB33_05025"
     CDS             complement(1447..2037)
                     /locus_tag="PLB33_05025"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOG62919.1"
                     /translation="MGKLFVILIGAIIFMLLSQKKAYAHCDTMDGPTVKDGIKALETK
                     NINYALKWILPAYDEELKKIFELSLKVRTLSPEAEELADRYFLESLVRIHRAGEGAGF
                     EGLKPHGEPIDEKVQAADKSIEIGNLSPLKGLVTDDEMHELEKLFEKVMALKDFEVND
                     VGAGRKYIEAYVSFFKFAEGEEHGHGHHTAHGHHIH"
     gene            complement(2215..3660)
                     /gene="proS"
                     /locus_tag="PLB33_05030"
     CDS             complement(2215..3660)
                     /gene="proS"
                     /locus_tag="PLB33_05030"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOG62920.1"
                     /translation="MAKKEKEFVKEITPMEEDFSRWYTDVIMKNELVDYSPVKGFMVI
                     RPYGYALWEKIQEYADGKFKATGHKNMYFPLLIPESLLNKEKEHVEGFAPEVAWVTQG
                     GNQELTERLVIRPTSETIICHMYAKWLNSYRDLPYLYNQWCSVVRWEKTTRPFLRTSE
                     FLWQEGHTLHETAEEAQAETLQMLDIYRQVAEDLMAMPVVVGQKSEKEKFAGAYATYT
                     MEALMHDGQALQAGTSHNLGQHFAKAFEIKFQGRNGQEQYPYSTSWGISTRLIGGLIM
                     VHSDNRGLVLPPKMAPVQVVIIPIAQKKEGVIEKANELFNRLKAKGIRVELDDNTEYS
                     PGWKFNQYEMKGYPIRIEIGPRDIENNQAVAVRRDELEKVILSLDNIEDTIYNMLETM
                     QNDLFEKAKAHREANTYVVEDYEKYKRDLPNRPGFAKMMWCGERECEDKLKEETGATI
                     RCIPFEQENLGDKCHICGKPAKHMIYAARAY"
     misc_binding    complement(3729..3926)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     assembly_gap    4268..4367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4368..5621
                     /locus_tag="PLB33_05035"
     CDS             <4368..5621
                     /locus_tag="PLB33_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOG62921.1"
                     /translation="GLSTPDTVILTNPNNVVGLVFKDPIEVSKEMVEELEDKTDVIIA
                     LAHLGLDESSTLTSKALAQEVEGIDLIIDGHSHSMLEAGQLENNTLIVQTKNYGQNLG
                     YVDIEILEGEVTGITARLLAAADTADVVPDPDLQKIIEDIEAANAEVFNEVIATTGVY
                     LDGVRENVRTKETNLGNLSADAARAATGADIAFVNGGGIREDIPVGDITKGKIAAIFP
                     FGNTIEVKKITGADLKAMLEWSVSDYPAAKGAFLQVSGLEFTFDPAKEIGSKVVEILV
                     GGEAFDEAKEYTVAINDFMSTGGDGYAMLADYDVLAIYGTYEEIIIDYLVANGTAGSE
                     VSGRIKVMETVVEEPEPVEPEPVEPAPVEPEPVEVVIYIVVPGDVLWKIAAKYNLTYQ
                     ELAEYNNIANPHLIFPDQVIKVPSK"
     gene            complement(5714..6553)
                     /locus_tag="PLB33_05040"
     CDS             complement(5714..6553)
                     /locus_tag="PLB33_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG62922.1"
                     /translation="MKLFSKLIGLLILASIIVYTYAREIEPVLLTIKYENIYSNYIND
                     KNDDIKIIQFSDAHISEFFDVKNLKKAVDRINSQNPHIVVFTGDLIDKYNEYNDKDNL
                     NKIWEVLKEINAPLGKYAVYGNHDYGGGAERAYKQIMEKSGFKVLLNKNEKLEEYNIN
                     IIGMDDSIFGTKDNKAIVDYLDENYFNIILSHEPDVAEYFLEYDADLFLTGHSHGGQI
                     NLPFINYTPPLAKKYVRGMYEVSNYRQTKIYVNVGLGTSQIPMRFMATPELTVITLKG
                     TSS"
     gene            complement(6666..6923)
                     /locus_tag="PLB33_05045"
     CDS             complement(6666..6923)
                     /locus_tag="PLB33_05045"
                     /inference="COORDINATES: protein motif:HMM:NF018016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOG62923.1"
                     /translation="MPASAVARVKGAVEDCIGKKVKFKLKKGKSRSHFNEGVILDTYP
                     SIFTVYIEKKDYKRIVSFNYIDILTNNVEFYICDDKETRIG"
     gene            7209..8144
                     /locus_tag="PLB33_05050"
     CDS             7209..8144
                     /locus_tag="PLB33_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOG62924.1"
                     /translation="MLDVFKSIDDTLFKLEEIEDGAWINLVNPTKEELDFIENKLSIE
                     GDLLRAALDEEESARLETEEGQVLILVDTPYIEKEDEHIIYETLPLGIIITDKNIITV
                     CLKNSIVLDQFVKNSIRTFYTYKKYRFLYQILYKNAQRYLLYLRQIDKMSNYVEEKLH
                     QSMKNKELIQLLDLSKSLVYINTSLKANQIVLEKILRINIIKKYTEDEDLLEDVIIEN
                     KQAIEMATIYSGILSGTMDAFASLISNNLNIVMKLLTSITILMAIPTMFASFYGMNVA
                     NIPLANSPFGFWIIIGISILTSVFAGFILAKKDLF"
     gene            8467..9336
                     /gene="ispE"
                     /locus_tag="PLB33_05055"
     CDS             8467..9336
                     /gene="ispE"
                     /locus_tag="PLB33_05055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOG62925.1"
                     /translation="MLRLNSYAKINLFLDIKGKLQNGYHIIKTVMQSIDIYDEITLIP
                     LNDNKIIIECSDKSIPINEKNTCYKAVLLLKKAYGINSGIQIYINKMIPAGAGMAGGS
                     SNAAAVIKGLNKMWKLNLSEQEMIDIGAQIGADVPFCLVGGTCLAEGIGDKVTDLNDF
                     QWDNILLVKPEFSISTAFVYKNLDTNYYNSNCEEYILKYITSYDYEKAAKCVANTLEK
                     VVEKYHPEINYIKKLMIDNGAISSIMTGSGSAVFALFKDNDSLNKAYEIAKKIYPSTF
                     KSKTSKYGVKFID"
     gene            9381..9926
                     /locus_tag="PLB33_05060"
     CDS             9381..9926
                     /locus_tag="PLB33_05060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOG62926.1"
                     /translation="MKKILIIILSIALITGCTNNSADIRENEVQEAVNETQETNDEAK
                     ANEPVMAPDFELESMDGSIIKLSETRDKNVIINFWYTGCQFCVVEMPDLQKLQDTYQD
                     DLLLLAINVGESKEKVEEFLDENNLSFTVLLDEDMNVAYDYGIRSFPTTIAVNKKGEV
                     IGGYIGMLTYEQMEQLYGFFE"
     gene            complement(9987..11012)
                     /gene="ltaE"
                     /locus_tag="PLB33_05065"
     CDS             complement(9987..11012)
                     /gene="ltaE"
                     /locus_tag="PLB33_05065"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOG62927.1"
                     /translation="MKFIDLRSDTVTMPTDEMRQAIANAEVGDDVYQDDPTVNKLEKI
                     AAEKVGKEAALFVPSGTFGNQLAIMTHTRRGNEVIVGKNNHIVVHEVGASAVISGVQL
                     RTLETVNGVMSPKDVLKAIRSDDIHEPETGLICVENAHGCGAVVPLSILKEIKEIAEN
                     HSIPVHMDGARIFNAAISSGVNAKEIAACCDSVMFCLSKGLAAPAGSMVAGSKEFIDR
                     ARKNRKLMGGGMRQIGILAAAGIIALEKMTDRLVEDHENAKYLAEELSKLPGVKVKRD
                     RLDIDMVFFEMGEDVIKESVLVKKFYEKNIKINGTEDGEYRFVTHIGVSKEDIDYVIN
                     CMKELIK"
     gene            complement(11063..11914)
                     /locus_tag="PLB33_05070"
     CDS             complement(11063..11914)
                     /locus_tag="PLB33_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOG62928.1"
                     /translation="MGILLKGKPVADSIVARVKDEAEELRRNNIIPKLKIIRVGERED
                     DLAYERAAVKRMKSANIDCEVLALPLNIDKETFEDEIKNVVNDNSVHGILVFRPLPKQ
                     LNEKDIRFIINPEKDIDCLNPINAAKILEGDESGFPPCTARAAMEILKYYNIDISGKE
                     ATVIGRSMVVGKPLSMMLLKENSTVTICHSKTENLPQVAKRADILIAAIGKSKMITKD
                     YIKENSTVIDVGINADDEGNITGDVNTEDCIEKAAFITPVPAGVGSVTTAVLADHVVK
                     ACRLLNK"
     gene            complement(11924..12553)
                     /locus_tag="PLB33_05075"
     CDS             complement(11924..12553)
                     /locus_tag="PLB33_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOG62929.1"
                     /translation="MNLTELSCKDFSFELSSKKPVPGGGGAAALAGSLGVALNMMVAN
                     FSKGKKKFIQYEKQHEDILKRGEKLRIDLLNLVEKDAEYFEPLSKAYSMPATTAEEKA
                     KKENQMQKCLKDACRAPLETMELVYEGIKLHEEILDISSLTIISDIGVGVQMLKAALN
                     SAYLSVLINLKYINDDQYVNNLKKRTEVLLTDGNKKADEIFEKVLITLR"
     gene            complement(12582..13190)
                     /locus_tag="PLB33_05080"
     CDS             complement(12582..13190)
                     /locus_tag="PLB33_05080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016080339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG62930.1"
                     /translation="MFRQTIYETDRLYLRILKPNYFRQVLEYYKRNHNFLKEWEPKRP
                     RDFYTAAYQKSQLKYEYNMFKRNKLVRFWIFKKEDNKLIGNICLSNIVMGNFRSCYMG
                     YKLDNDEINKGYMTEAIRKVIRIMFEDFGLHRIEVNILPRNVRSLRVMEKLNFEKEGY
                     SKRYLEINGKWEDHVHFAIYQDSFEEQKCAETARKLIFESNI"
     gene            complement(13428..14090)
                     /locus_tag="PLB33_05085"
     CDS             complement(13428..14090)
                     /locus_tag="PLB33_05085"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOG62931.1"
                     /translation="MRYCNNCNVSVVGKRKLCPLCQDRLIGDKPQEEVFPKISFVYRE
                     YSSFFKIMLLVSIIIATVSVAVNILIPESGAWSFLILGGLGSVWASLINLINQRKNIP
                     KNIVYQVMTISVISLIWDLLTGWKGWSINYVIPSVCVFAMISMAIISKIRKLYIEDYI
                     LYIIIDALFGIVPIIFVIFGLLDVLYPSIICISTSIISLSTIIIFEEKSLIAEIKRRL
                     HV"
     gene            complement(14090..15376)
                     /locus_tag="PLB33_05090"
     CDS             complement(14090..15376)
                     /locus_tag="PLB33_05090"
                     /inference="COORDINATES: protein motif:HMM:NF018902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62932.1"
                     /translation="MKNKRISEWSKLDNAAKIFPANSKKSDTKVFRFACELFDTVEPK
                     VLQQALDITLEAFPLYLSVIKSGLFWHYFEKSHFKALVTEETLPPCSTLYDKNNKTLL
                     FRVMYYRKRISFEVYHALADGAGALQFFRRLIFHYITIKYKYALRDKSPVLNIESSKT
                     QRLDDSFKRYYKEEKILTKKKRIKAYKIKGAKIPEYRIRIIEGVIPVDEILPKVKENK
                     TSLTIFLAAIFMCAINEEIPSRLKRKPVVLSIPVNLRNFYSSQTARNFFGVINVDYNF
                     AEGSGDLEDVIEVLKKKFKENLKPEIMARRINKLSSLEHNYVLRVIPLAIKNLILRIA
                     YAIADRSYTSTLSNVGIINMPDDIKPFIYSFDIFVSTDKIQACVNSFENILRISFTSA
                     FVSTEIQRRFFKTLTDMGINVTIESNIIDEEQEVLL"
     gene            complement(15377..>16279)
                     /locus_tag="PLB33_05095"
     CDS             complement(15377..>16279)
                     /locus_tag="PLB33_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOG62933.1"
                     /translation="VVRAALKALSYNEYDIKENLQGYRNFVNLTRRQILKPSLKTYDF
                     QISSKNHNIPVRVYSPEANFDCPLLLFFHGGGWVAGSIESYNKICTNMARLTNHKVVS
                     VDYRLAPENPFPAGLEDCYETAKAFIANDEINDKEVTLIGDSAGGNLAAALSLLARDR
                     KEFKVDKQILIYPSTYNDYTENSPFKSVIENGTDYLLTSKRIRDYMDLYKTKEDDIYS
                     PYFAPLLSRDFTKQPKTLIITAEYDPLRDEGEEYGRKLREAGNYVEIYRIKDALHGFF
                     ALPPKFPQVKLCYDIINRFLCEVN"
BASE COUNT         5085 a         2959 c         2568 g         5567 t
ORIGIN      
        1 agttagcatt taaaaagacc tcctttcatg caatatttat atattcttgt aaattatagt
       61 aattattgat ataattgagc ttaagaaaaa tatgacatac tttaccttga agacgaaaat
      121 gattaagaaa taataaggat aattgtttgt aatagattaa gataaaggag gtgataaata
      181 ttaaaagaaa gattatcatt agtgtagttt ttgcattgtt ggtaatactc tcggcaggat
      241 gtcagcaaaa tgataaagat gtcggtgcac tcaatgacac aaagataata agagcagaag
      301 aatggaagaa tcaacatccg gacatttatg catcttttat gactaatgcc gaaatgaagg
      361 aaacaaaata cggtggctct gtgcctgatg actatcttga agaatatcct aatcttaagg
      421 tattttatga tggatatgga tttagcaaag aatacttagg ggcaaggggt catatttatg
      481 ctttggaaga tgtgataaac acttcaagac caaaaggagg agccagctgt ttagcctgca
      541 aagcagctga tttcaatgta cagcttgcca atgaaggtga aggggtcaac aaaattccat
      601 ttaatgaatt tgttgaagca aatcccgaaa tggaaggtat cagctgttat gactgtcaca
      661 gaaacactcc gggagaagta aacataacaa gaacgcatct tacacagggt ttaacttttg
      721 tcgaagaaga aaagtcagga aatttagctt gcgcacagtg ccatgtagaa tattatttag
      781 ctcctgatac caataaagta attttacctt ggcataacgg aattgataca gataatatga
      841 ttgaatacta tgatgaaata aacttcaggg attatgaaca tccgactaca ggtgcgcaaa
      901 tattaaaggc acagcatcct gaatttgaaa cctttaaagg aagtatacat gatgctcaag
      961 gtttaacttg tattgattgt cacatgccta gaatagctgg agaagaaaat cttaaatctc
     1021 atcaatggac cagcccgctt aaatctaaag aaggattgac taaatcttgc attccttgtc
     1081 atggcggaga acctgaagac atcataagca aggtagaagg agttcaagaa tcagtctaca
     1141 agaaaaccaa tgaggtaagt gatgagcttt taactttcat caatcgatta ggaaaagccg
     1201 ttgaagatgg agatttgcct gatgaagtat tagaacagct gagaggcatc cacagaaatt
     1261 cacaatttat gtgggacttt gtctttgtag aaaatagtga gggattccat aactctcaaa
     1321 aaacacataa aaacctggat aatgcacgtg aattgattac agaaggaata gaaattttag
     1381 aagagcatgg aaaatagaat aaaaacatca cccttttgtt aattcaatcg ggtgatgctt
     1441 tttattctag tggatatgat gtccatgcgc agtgtgatgt ccgtgaccat gttcttcgcc
     1501 ttccgcaaat ttgaagaagc ttacatatgc ttcaatatat tttcttcctg cccctacatc
     1561 atttacttca aaatctttga gcgccataac tttttcaaac agtttttcaa gttcatgcat
     1621 ttcatcatca gtcactaatc cttttaaagg tgacaggttt ccaatttcaa tacttttatc
     1681 ggctgcttga actttttcat caattggctc gccgtgcggt tttaatcctt caaatcctgc
     1741 cccttctcct gctctatgta tgcgaactaa actttcaaga aaatatctgt cagcaagttc
     1801 ctctgcttca ggacttaacg ttcttacctt taaacttaat tcaaatattt tctttaattc
     1861 ttcatcatat gcaggtaaaa tccactttaa tgcatagtta atattttttg tttctagtgc
     1921 tttaatacca tccttaacag ttggaccatc cattgtgtca caatgagcat atgccttttt
     1981 ctgcgatagg agcataaata ttattgctcc gataagaatt acaaataatt tccccatgtc
     2041 aattctcctt attatttact ttattatttt atacccttat tgtacaaaac taatcgatgt
     2101 cattctttga tttatatcaa tttaataatt aaataaaaac gaaatccctc gcctctgttc
     2161 tgaatgacag tcatcctgaa gaagtgaagg atttctataa taaggttggg acatctaata
     2221 tgctcttgct gcgtaaatca tatgttttgc aggctttccg catatgtggc acttatctcc
     2281 taagttttcc tgctcgaaag gaatacatct tattgttgca ccggtttctt cttttaattt
     2341 atcttcgcat tctctttcac cgcaccacat catttttgca aatccgggac ggttgggtaa
     2401 atctctctta tacttttcat aatcttcaac tacataagtg tttgcttctc tgtgagcttt
     2461 tgctttttca aataaatcat tttgcattgt ttcaagcatg ttataaattg tatcttcaat
     2521 attatccaga gaaagaatta ctttttccaa ttcatctctt cttactgcaa ctgcttgatt
     2581 attttctata tctcttggtc ctatttcaat acgaatagga taacccttca tttcatattg
     2641 attgaatttc catccgggtg aatattctgt attgtcatca agctctactc ttattccttt
     2701 tgcttttaat ctgttgaata attcattagc tttctcgata acgccttctt tcttttgagc
     2761 tattggaatt attacaacct gcacgggtgc cattttagga ggaagtacca accccctgtt
     2821 atcagaatga accattatta gaccaccgat taatctagtg gaaattcccc acgacgtgga
     2881 atagggatat tgctcttgcc cgtttctgcc ttgaaattta atttcaaaag cttttgcaaa
     2941 atgctgccct aaattatgag atgttcccgc ctgcagagcc tgaccgtcat gcatcaatgc
     3001 ttccatagta tatgtggcat aagcaccggc aaatttttct ttttcgcttt tttgacctac
     3061 gacaacgggc atagccatta agtcttccgc cacctgtcta tatatatcaa gcatttgtaa
     3121 agtttccgct tgagcttctt ctgctgtttc atgaagggtg tgaccttcct gccacaaaaa
     3181 ttccgatgtt cttaaaaaag gtcttgttgt tttttcccac cttacaacac tgcaccactg
     3241 gttatacaaa tatggcaaat ccctgtatga attaagccat tttgcataca tatgacatat
     3301 aatggtttct gatgtaggcc ttattacaag cctttctgtc aactcctgat ttcctccctg
     3361 agtaacccat gcaacctcag gagcgaaccc ttccacatgt tctttttcct tgtttaacaa
     3421 actttcagga attaacaatg gaaaatacat atttttatga cctgtcgctt taaattttcc
     3481 atccgcatat tcttgaattt tctcccacaa agcatatccg taaggtctta taaccataaa
     3541 acctttaacc ggcgaataat caaccaattc gtttttcatt attacatctg tataccacct
     3601 gctgaaatcc tcttccatag gcgtaatttc ttttacaaat tctttttctt tcttagccat
     3661 accttctcct ttcataacac agactcataa ctccaaattc ataaccttac aaaaataaaa
     3721 ccgcctccgt aagaggcgga tatccgcggt accactctaa ttactgtttt aaaaacagca
     3781 ctcattgtat ataagggact tccctttgag tttttaccct caaatactcc aaggctggtt
     3841 caaaatacag ggggtcgaaa atcttccacc gatgatttcc tctctagaga ccgtgtaaat
     3901 ttactattcc tcttcatcgc aaattattaa tttgtataca taatataaaa atatataaac
     3961 tttgtcaaca gatttaagat attttttatg ctaactttgt tcagtatcac gtatttacca
     4021 caccacgata taaatacgaa tgttattaat aacaatgaca ggctgattaa aagtacagtg
     4081 actctcaaat ttgcaaatta ttcaaaagtt gtcttgaaat tccagccttc tgaatgtata
     4141 atggatgtag gtcttaacat aacaaaaagg aggaaaattt aatgaagaaa gtattaagtt
     4201 tagttttagt tgtattactg attctaagca gtttcagctt tgtatttgca gaagatgctg
     4261 aaaccgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt ctttctacac
     4381 ctgatacagt aattcttaca aatcctaata atgttgttgg tttagtattt aaagatccta
     4441 tagaagtatc aaaggaaatg gtagaagaac ttgaagacaa aacagatgtt ataattgcat
     4501 tggctcattt gggtcttgat gaaagttcaa ctttaacatc aaaggcttta gctcaagaag
     4561 tagaaggaat cgatttgata atagacggac acagccattc aatgttagaa gccggtcaat
     4621 tagaaaataa tacattgatt gttcaaacaa aaaattacgg tcaaaattta ggttatgtcg
     4681 atatagaaat tttagaaggt gaagttacag gtattacagc aagacttctt gcagctgcag
     4741 acactgcaga tgttgttccc gaccctgatt tgcaaaaaat aatagaagat attgaggctg
     4801 ccaacgcaga agtatttaat gaggttatag caacaacagg cgtatactta gacggagtaa
     4861 gagaaaatgt cagaactaag gaaacaaatt taggaaacct atctgctgat gcagcaaggg
     4921 ctgcaacagg tgctgatatt gcctttgtaa acggcggcgg cataagagaa gatatacctg
     4981 ttggtgatat aacaaaaggt aaaatagcag ctatattccc atttggcaat acaattgaag
     5041 taaagaaaat tacaggtgct gacctgaaag caatgcttga atggtccgta agcgattatc
     5101 ctgcagcaaa gggagccttc ctgcaagtaa gcggattgga atttacattt gaccctgcta
     5161 aggaaattgg ttccaaggtt gtagaaatat tagtcggcgg agaagcattt gatgaagcta
     5221 aggaatatac ggttgctata aatgacttta tgtcaaccgg cggagacgga tatgcaatgc
     5281 tggctgatta tgatgttctt gcaatatacg gtacctatga agaaatcatc atagattatc
     5341 ttgttgccaa cggtactgca ggaagtgaag tttcaggaag aattaaggtt atggaaactg
     5401 ttgttgaaga acctgaacct gtagagcctg agcctgttga acctgctcct gtagagcctg
     5461 aaccggttga agtagtgatt tacatagttg taccgggaga tgttctatgg aaaatagcag
     5521 caaaatataa tttaacttat caggaattgg ctgaatacaa caatattgca aatcctcatt
     5581 taattttccc agaccaagta ataaaggttc catccaagta aaatgaaata tatgaaggct
     5641 ttgttaacat aacaaagcct tcaatttttt tttacaaacc cttttatgtc atccttagcg
     5701 taagcgaagg atctcatgat gatgtgcctt ttaaagtaat aaccgtcaat tccggtgttg
     5761 ccataaaacg catcggtatt tgagatgttc ccaaacctac atttacatat attttcgtct
     5821 gcctgtaatt ggaaacctca tacatccctc ttacatactt ttttgccaag ggcggagtgt
     5881 aattaataaa tggcaggttt atttgtcctc cgtggctgtg tccggttaaa aacaaatctg
     5941 catcatattc cagaaaatat tccgctacat ccggctcatg acttaatatt atattgaaat
     6001 aattttcatc caaatagtcg actatggctt tattgtcttt tgttccaaaa attgaatcat
     6061 ccatccctat tatattaata ttatattctt caagcttttc atttttgttt aatagcactt
     6121 taaaaccgct tttctccata atttgcttat aggctctttc tgcgccgccg ccgtaatcat
     6181 ggttgccata tactgcatac ttccctaatg gagcatttat ttcctttaaa acttcccata
     6241 ttttgtttaa attgtcttta tcattgtatt cattgtattt atctatcaaa tcacccgtga
     6301 aaactacaat atgcgggttt tggctgttta ttctatcgac tgcttttttt aaatttttta
     6361 catcaaaaaa ttcagatata tgggcatctg aaaattgtat tatttttata tcgtcatttt
     6421 tatcatttat ataattactg tatatatttt catacttaat tgttaataaa accggttcaa
     6481 tttctcttgc atatgtataa acaataattg aagctaatat taaaagaccg attaattttg
     6541 aaaataattt cacttaacta ctcctaatca tttatataat caaagtataa caaattttca
     6601 caaaaaaata tagcgaaact tcttaattaa taatttttta ataagttagt ttcactatta
     6661 aaatgctatc cgattctggt ctctttatca tcacatatat aaaattcaac attatttgtt
     6721 agaatatcta tataattaaa tgatacaatc cttttatagt ccttcttttc aatgtagact
     6781 gtaaatattg aaggataagt gtctaaaatt actccttcat tgaaatgaga cctgctttta
     6841 ccttttttaa gcttaaattt tacctttttc ccgatacagt cttcaactgc tcctttaact
     6901 cttgcaacag cacttgcggg caaataatca ccttctatac taaatttgat gaaaaacgaa
     6961 aaaataccat ttatttgtta atatcttttc cattaacata aaaattatgt gcattatctt
     7021 aaaattgccg gtatatttaa cttgaaaaac atattgatat attgtattat tataaagcag
     7081 taaaaaagta aaggatgtga agtaatggac gaggtcccta aggtcgaatc tatattaact
     7141 gaactctcta ttactaatat tatatcaatt tagtaataga gaaatctatt attaaattga
     7201 ggtgaaaaat gcttgatgta tttaaatcaa ttgatgacac cctattcaag ttagaagaaa
     7261 ttgaagatgg tgcttggatt aacttggtta atcctacaaa agaggaatta gactttattg
     7321 aaaataagct gtctattgaa ggagatcttt taagagcagc tttggacgaa gaggaaagtg
     7381 cccgtttaga aacggaagaa ggtcaagttt taatattggt tgatacgccc tatattgaaa
     7441 aagaagatga acatattatt tatgaaacac tccctcttgg aattataata accgataaaa
     7501 atataataac agtatgttta aaaaattcaa ttgtacttga ccaatttgtg aaaaacagca
     7561 ttcgtacttt ctacacttat aaaaaatacc gttttttata tcaaatacta tacaagaatg
     7621 ctcaaagata tttactttat ttaaggcaaa ttgacaaaat gagcaattat gtagaggaga
     7681 agctgcatca atcaatgaaa aacaaggagc ttatacagct cttggactta tcaaagtctt
     7741 tggtatatat taatacttct cttaaagcaa accaaatagt actggaaaaa atccttagaa
     7801 tcaatataat aaaaaaatat accgaggatg aggatttatt ggaagatgta attattgaaa
     7861 ataagcaagc cattgaaatg gccacaattt acagcgggat tttaagcggt acaatggatg
     7921 catttgcatc tcttatatca aataatttaa atattgttat gaagctctta acatccataa
     7981 caatattaat ggctataccg accatgtttg caagctttta tggaatgaac gttgctaata
     8041 tccccctcgc aaattcgcct tttggtttct ggataataat tggtatttca atactgacat
     8101 cagtatttgc aggatttata cttgcaaaaa aagatttgtt ctaaggtatg gggacacacc
     8161 ttcaaagtcg ggtcactcta tgtgggataa cttttgaagg aatgtcccca atttgagggg
     8221 gacacacaaa aaatgaataa cacccgtttc tcatcagtta cacaccagag gacgggtgtt
     8281 attcattcat agtttcaatt tccgttttcg ggggtaccac cctctttctt tatttgcttc
     8341 attaaattta tacccaataa tttaaatttt aatcataaaa aactattaaa tttataaata
     8401 attgtatttt ttgttgttga aaaataaatt atgttatata atgttgaaaa aaattgagag
     8461 atggctatgt tgagattaaa ctcatacgca aaaattaatt tatttttaga tattaaggga
     8521 aaacttcaaa acggttatca tataattaag actgttatgc agtctatcga tatttatgat
     8581 gaaataaccc ttattcccct taatgataat aaaattatta ttgaatgctc cgataaatct
     8641 attccgataa atgaaaagaa tacctgttat aaagctgtgc ttttattaaa gaaagcttac
     8701 ggtataaatt caggcataca aatatatatc aacaaaatga ttcctgcggg agcgggcatg
     8761 gcaggaggca gttccaatgc agctgcagta ataaaaggct taaataaaat gtggaagctt
     8821 aatttatcgg aacaagaaat gatagacatt ggagcacaaa tcggtgctga tgtgcctttt
     8881 tgcttagtag gaggtacttg tctggcagaa gggattggtg acaaagttac tgatttaaat
     8941 gatttccaat gggataatat tttattggtg aagccggaat tttcaatttc gactgcattt
     9001 gtttataaaa accttgatac caattactat aattctaatt gtgaagaata catactgaaa
     9061 tatattacat catatgatta tgaaaaagct gctaaatgtg ttgcaaatac attagaaaaa
     9121 gttgttgaaa agtatcatcc cgaaatcaat tacattaaga aattaatgat tgacaatggt
     9181 gcaatatctt ccataatgac cggttcaggc tctgctgtat ttgctctttt taaagacaat
     9241 gattccctga ataaggcata cgaaattgct aaaaaaatct atccttctac atttaagtcg
     9301 aaaacatcta agtatggagt taaatttata gattagacaa attcgtatct gggtatcagt
     9361 taattaataa ggagattacc atgaaaaaaa tactgattat tattttatca atagcattga
     9421 taaccggatg tacgaataat tcagctgata ttcgtgaaaa tgaggtgcag gaagctgtca
     9481 atgaaactca agaaaccaat gatgaagcaa aagcaaatga gcctgtaatg gcaccggatt
     9541 ttgaacttga gtctatggac ggctcaataa taaaactgag tgaaaccaga gataagaatg
     9601 taattataaa tttctggtat acgggatgtc aattttgcgt agtggaaatg cctgatttgc
     9661 aaaaacttca ggatacttac caagatgacc ttttgttatt ggcaataaat gtaggtgaga
     9721 gcaaagaaaa agttgaagag tttttggatg aaaacaacct gagctttact gttttattgg
     9781 atgaggatat gaatgtagct tatgattacg gcatccgctc cttcccaaca actattgcag
     9841 taaacaaaaa aggtgaagtt ataggcggtt atatagggat gctcacttat gagcaaatgg
     9901 aacagctgta tgggtttttt gaataggaac atctgtgcca tactggtgcc aggcaccttt
     9961 taaggcgcct ggcaccagtg gtttaatcat ttaatcaatt ctttcataca atttataaca
    10021 taatcaatat cttcctttga tactcctata tgtgttacaa agcggtattc accatcctcg
    10081 gttccattta ttttaatatt tttttcataa aactttttaa ctagaacact ttcttttatt
    10141 acatcttctc ccatttcaaa aaataccata tcaatgtcaa gcctatcccg ttttacttta
    10201 actccgggca gttttgaaag ttcttccgct aaatatttag cattttcatg gtcttctaca
    10261 agtctgtcag tcattttttc caatgcgata attcctgctg cagccaatat tcctatttgc
    10321 ctcataccgc cgcccataag ttttctgttc tttcttgccc tgtctataaa ttccttgctt
    10381 cctgcaacca ttgaacctgc aggtgcagct aatccctttg ataagcagaa cattacggaa
    10441 tcacagcatg ccgcaatttc ttttgcattt actcctgatg atattgcagc attaaaaatt
    10501 cttgccccat ccatatgaac cgggattgaa tgattttccg caatttcttt tatttctttc
    10561 aatattgaaa ggggtactac tgctccgcac ccatgagcat tttcaacaca tatgagacct
    10621 gtttccggct cgtggatatc atcggacctt atagctttta aaacatcttt gggagacata
    10681 acaccgttta ctgtttccaa agttctaagc tgcactcctg aaataacagc agacgctcca
    10741 acttcatgca caacgatatg attatttttt cctactatta cctcattgcc tcgtctggta
    10801 tgcgtcataa ttgccagttg atttccgaat gtaccgcttg gtacgaacaa ggctgcttct
    10861 ttacccacct tttcagcggc aatcttctcc aatttattaa ccgtcgggtc atcttggtaa
    10921 acatcatctc caacttctgc atttgcaata gcctgtctca tttcatcagt aggcattgta
    10981 acagtatcgc ttcttaagtc tataaatttc atttttccac cctctatttt tatttttaat
    11041 aaggtatgtt aattaaccgt tattacttat ttaataacct gcatgctttt acaacatggt
    11101 ctgccagcac ggcagttgta accgagccca cccctgcagg tacgggagtt ataaatgctg
    11161 ctttttcaat gcagtcctcc gtgttgacat ctccggttat attcccttca tcatcggcat
    11221 tgattccaac atctattacg gttgaatttt cctttatata gtcttttgta atcattttag
    11281 actttccaat ggctgctatc agaatatctg ctcttttggc aacttgtggc aggttttctg
    11341 tttttgaatg gcatatggtt acggttgaat tctctttaag aagcatcatt gacaaaggtt
    11401 tccctaccac cattgacctc cctattactg ttgcctcctt gccgctgata tctatattat
    11461 aatatttgag aatttccatt gctgcccttg ctgtgcatgg aggaaatccc gattcatctc
    11521 cttccaatat tttagcagca tttataggat taaggcaatc aatatctttt tccgggttta
    11581 ttataaatct gatatctttt tcatttagct gcttgggaag aggtctaaat accaaaatac
    11641 cgtgaacact gttatcattt acaacattct ttatctcatc ctcaaaagtt tccttgtcta
    11701 tatttaaggg caatgccaaa acttcacagt caatgtttgc acttttcatt ctttttacag
    11761 ctgctctttc ataagccaaa tcatcttctc tttcaccgac acgaattatt ttcaattttg
    11821 gaataatatt atttcttcta agttcttccg cttcatcttt tactctggct acaatactgt
    11881 ctgccaccgg tttgcccttt aaaagtattc ccattttgcc tccttaacgc aaagtaatta
    11941 aaactttttc aaatatttca tcagcctttt tatttccgtc cgttaaaaga acttctgttc
    12001 ttttttttaa attatttaca tattggtcat catttatgta tttcaaatta attaatacac
    12061 ttaaatatgc actgttaagt gctgccttta acatctgaac tccaacacct atatcactaa
    12121 taattgttag ggatgaaata tccagtattt cttcatgaag tttaattcct tcataaacta
    12181 actccatcgt ttctaaagga gctctgcatg catccttaag acatttttgc atttggtttt
    12241 cttttttagc tttttcttcg gctgtagttg caggcatact gtatgctttt gaaagaggct
    12301 cgaaatattc agcgtctttt tctaccaaat tcaataagtc tatgcgaagt ttttctcccc
    12361 ttttcaaaat atcttcatgt tgtttttcat attgaataaa ttttttttta ccttttgaaa
    12421 aatttgcaac catcatattg agggcaactc ccaaagagcc ggcaagtgct gctgcccctc
    12481 cgccaccggg caccggcttt tttgatgaca gttcaaaaga aaaatctttg catgataatt
    12541 ccgtcaaatt cataaaatca cctctcatat attataacca attatatatt actttcaaag
    12601 attaattttc gcgcagtttc tgcacatttt tgttcttcga aggaatcttg atatattgca
    12661 aaatgaacat gatcttccca tttgccgttg atttccaaat atctctttga ataaccttcc
    12721 ttctcaaagt tcagtttttc cataactctc aatgacctta cattccgagg cagtatattt
    12781 acttcaattc tgtgcaggcc aaagtcctca aacataattc gtataacttt cctgattgct
    12841 tctgtcatat atcccttatt aatttcatca ttgtccaact tgtagcccat ataacaggac
    12901 ctaaagtttc ccataactat attgcttaaa caaatattgc ctattaattt gttatcttcc
    12961 tttttgaaaa tccaaaatct taccagtttg tttcttttaa acatattgta ttcatatttc
    13021 agttggcttt tttgatatgc tgcggtataa aaatcccttg gtctttttgg ctcccattcc
    13081 tttaaaaaat tatgattcct tttatagtat tccaagactt gtctgaaata attcggtttt
    13141 aaaattctta aatataatct gtctgtttca tatattgttt gtctaaacat cttattttcc
    13201 tttcatatgt gtcatttaat ttcataatcc ttcttatata ggcagcagat cactttatca
    13261 tgagatcctt cgctatcgct caggatgaca aagcgtatta aggcgcaacc cttgttattg
    13321 ctattaataa actacgtgtc attctgagag ctttagctcg aagaatctca tttttttaac
    13381 catgagatac ttcgctactc tcatgatgga aaaaatattt agaagtatta cacatgcagc
    13441 cttcttttta tttccgctat caaacttttt tcttcaaaaa ttataattgt tgacagtgaa
    13501 atgatgcttg ttgatataca aataattgac gggtacaaaa cgtccagtaa accaaaaatt
    13561 acaaagatta tggggactat cccaaacaat gcatctatta ttatatataa aatataatct
    13621 tcaatataga gttttctgat ttttgaaatt atagccattg atatcatagc aaacacgcat
    13681 acggaaggta ttacataatt aattgaccaa cctttccaac ccgttaataa atcccatatt
    13741 aaagatatta cagatatggt cataacctgg tatacaatgt ttttgggaat atttttgcgt
    13801 tggtttatga ggttaataag acttgcccaa actgagccca atcctcccaa tattaaaaat
    13861 gaccacgctc cgctttccgg tatcaatata ttaaccgcaa ctgaaacagt ggcaattatt
    13921 attgaaacta agagcataat tttgaaaaat gaagagtatt ctctataaac aaatgagatt
    13981 ttcggaaaaa cttcttcctg gggtttgtcg cctatcagcc ggtcctgaca caaaggacag
    14041 agctttcttt ttccgactac gcttacatta cagttgttac aatatctcat taaagcaaca
    14101 cctcctgttc ttcatcaatt atattggact caatagtaac gtttattccc atatcagtta
    14161 atgttttaaa aaaccttctt tgtatttcgg tacttacaaa tgccgaagta aaactgattc
    14221 ttagtatatt ttcaaaagag tttacgcaag cttgtatttt gtcagtactt acaaatatat
    14281 caaatgaata tatgaaaggt ttaatatcat caggcatatt gataatacca acattggaca
    14341 atgttgaagt atagcttctg tctgctattg catatgcaat ccttaaaatc agatttttaa
    14401 ttgccagcgg aatcactctt aaaacataat tatgctctaa ggatgataac ttgttaattc
    14461 ttcttgccat aatttcgggc tttaaatttt ctttaaattt tttctttaaa acttctatta
    14521 catcttctaa atcaccgctt ccctcagcaa aattatagtc aacgtttatc actccaaaaa
    14581 aattacgggc tgtttgtgag gaataaaagt ttctaagatt tacgggaatg cttaatacta
    14641 cgggctttct ttttaatctt gatgggattt cttcatttat ggcacacata aaaattgcag
    14701 ccagaaaaat tgtcaaagaa gttttatttt ccttaacctt aggcaatatt tcatcaacag
    14761 gaataactcc ttcaattatt ctgatacggt attcgggtat tttcgcaccc tttattttat
    14821 atgctttaat tctctttttt ttagttaaaa ttttttcttc tttatagtat cttttaaaac
    14881 tgtcatccag tctctgtgtt tttgaagatt ctatatttaa aacaggcgat ttatctctta
    14941 aggcatattt atatttaata gttatgtagt gaaaaattaa ccttctgaaa aactgcaagg
    15001 ctcctgctcc gtcagctaag gcatgatata cttcaaaact tatgcgcttc ctgtaataca
    15061 taaccctaaa cagcaatgtt ttattgtttt tatcatataa tgtggagcag ggaggtaaag
    15121 tttcttctgt aacaagggcc ttaaaatgac ttttttcaaa ataatgccaa aaaagcccgc
    15181 tctttattac tgacagataa agagggaatg cttccaaagt aatatcaagt gcctgttgaa
    15241 gcaccttggg ctctaccgta tcaaataatt cacatgcaaa cctgaatact tttgtatcgc
    15301 tttttttgct gtttgcaggg aatattttag ctgcattatc taatttgctc cattcggata
    15361 ttcttttatt tttcaatcaa ttcacctcgc aaagaaaccg gttgattata tcatagcata
    15421 atttaacctg aggaaattta ggaggaagag caaaaaatcc gtgaagggca tcttttattc
    15481 tgtatatttc aacataattt cctgcttccc taagttttct gccgtactct tcaccttcat
    15541 cacgcaaggg gtcatattct gcagttataa ttaatgtctt cggctgcttt gtaaagtccc
    15601 tggaaagcaa aggtgcaaaa taggggctat atatatcatc ttccttggtt ttatacaaat
    15661 ccatatagtc tcttattctc ttggaagtaa gcaaataatc ggttccgttt tctataacgg
    15721 acttgaaggg agaattttcc gtgtagtcat tgtatgttga cggatatatc aatatttgtt
    15781 tatcaacctt aaattctttt ctatcccttg caagcaagga taaagctgca gccaaattgc
    15841 ctcctgcact atctcctatc aaagttactt ctttatcatt aatttcatca tttgcaataa
    15901 atgcttttgc tgtttcataa caatcctcta atcctgcggg aaagggattc tccggtgcca
    15961 gcctgtaatc gacggacaca accttatggt ttgtcaacct tgccatattt gtacaaattt
    16021 tgttgtaact ttctatactg cctgccaccc atccgccgcc atgaaaaaat aaaagcaaag
    16081 gacagtcgaa attcgcttcg ggagaatata cccgaacagg aatattgtga ttttttgagc
    16141 ttatttgaaa atcgtacgtt ttgagcgaag gttttaaaat ttgccttcgt gttaaattta
    16201 caaaattcct gtagccctgc aagttttctt ttatatcata ttcattgtag gacagagctt
    16261 taagtgcagc tcttacaac
//
LOCUS       DAOSHP010000036        16056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_2984_length_16056_cov_8.334917, whole genome shotgun sequence.
ACCESSION   DAOSHP010000036 DAOSHP010000000
VERSION     DAOSHP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16056
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_16056_cov_8.334917"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLB33_05100"
     CDS             complement(<1..610)
                     /locus_tag="PLB33_05100"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG62934.1"
                     /translation="MEKIIVKKSPGLKGNIRVDGSKNSILPILAATLLCKEECIIHDI
                     PDLQDVKIMCKLLEELGAKVEKEGMNSLKITAENIKTCEAPYELISKMRASFLVMGPL
                     LTRCHNAKVCMPGGCAIGTRPIDLHLKGFKYLGSEVKSESGYIHATAEKLIGRDIYLD
                     FPSVGATENIIMAATLAEGETILENAAEEPEIVDLANFINSMG"
     gene            complement(1129..3219)
                     /locus_tag="PLB33_05105"
     CDS             complement(1129..3219)
                     /locus_tag="PLB33_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG62935.1"
                     /translation="MLVTKWKIFKKKYITKFSAFILLIVFLMIGVSSALNIYLKVKNY
                     ESIAVKNYLESDTISNDLKYAVNRLEFILRVYKNEEYVLSGGTVEKLEIEDNWKLANL
                     YNNYLAENNYDDNSETKELFFIEKQQEIQEIKESIISSDLANYEKIISELNLPQGYIY
                     YATDGENEITNTGNPNKEFYLLRNAYIVLSEKGVELSPENGGGVTDPLTDIFESETFN
                     KNMRIYAAIEDDALLHRANRWNSDRQVLIRNTGVIVICILLVIANFSYLISSAGKMAG
                     SDEIHLTSFDNLYTDINLLLITGIAAVCRLEFYKVLNIKTISENLMRLVMLISTAAVS
                     ALILILVLSLVRHIKKRTILKHSVIYIILSKLVDVLIQMAASGPLMYKTMGAAILVIF
                     ASVYAADKPLLIAGILFISTYIIYNKVTHFHKIQEGLQVAKSGDYDFKIRLEGKGEFR
                     QLSEDINDLTSGLKTAVENEVRSERLKTELITNVSHDIKTPLTSIISYVDLLKREGLN
                     SDNAPKYLEVLERKSNRLKNLTEDLFEAAKATSGSIEPKFERVHINSLIEQLLGEMDE
                     KISESNLIFKVSATNEKIYAMADGRLLSRVMENLLSNIFKYTLKGSRVYIEISEDEDN
                     VTISFKNISSTELNIPVNELMERFKRGDESRSSEGSGLGLAIAKSLMEVQEGSLELSI
                     DGDLFKAEIRLNKF"
     gene            complement(3191..3886)
                     /locus_tag="PLB33_05110"
     CDS             complement(3191..3886)
                     /locus_tag="PLB33_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG62936.1"
                     /translation="MNNIMVCDDDKEIVDAIEAYLKSEGYNILKCYSGKQAIEIVEKN
                     EVHLIIMDIMMPEMDGITATTKIREIKNIPVIMLSAKSEEADIVLGLNMGADDYITKP
                     FNPLELIARVKSHLRRYTALGGALDAEKVGLYKTGSLMIDDERKEVTVDGEIVKLTPV
                     EYKILLLLTKNKGVVYSIDEIYENVWNEPSFNPENTVAVHIRRIREKIEIDPKNPRYL
                     KVVWGVGYKVEDI"
     assembly_gap    3905..4004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4005..5557)
                     /locus_tag="PLB33_05115"
     CDS             complement(<4005..5557)
                     /locus_tag="PLB33_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOG62937.1"
                     /translation="MREILIGLIFALTFTPVNAEETFTNTIEYLQPDDTNDATYPEIP
                     QLPEIDKSTELIKYSPEYNKYINSAYGYEITIPNNLTLNEDIVSVKSRFESDDLVVEV
                     FYDNFYNTINSHATHRNYCNRGILKNPEFKITDEYNHSIGGLSGHVTLFERRKVKTKE
                     PDRNYYATITFEKNTYESITIFIKSAAPINIDDIMPEFKFTAKTETLKNDKVFHPEPK
                     NFDEKTRDFYDKYFINNEKTAFGIFEPTFPLYAYRLLQIEKMLDYNFPVALLYNNFSL
                     PFKTDYMNKAKELGKVVEYGLYTIDIINGREKDITLDILEGKYDDYLDNLAQSFNEYD
                     YPVLFRLNNEMNGEWVLYCSHKVGKDTDLFIDCWRYIYDKFEEHGVDNLIWVWNPNEK
                     SFPDFSYNNYLCYYPGNKYVDIVGLTSYNTGNYYRGEIWRSFSEAYDHFYFDYIDRFT
                     HPMMITEFSCASAGGDKAQWFEDMFNMIGKYDRIKLAVLWNGQDYDPNKEEKTVSRNY
                     RIDLEESVI"
     gene            5725..6147
                     /locus_tag="PLB33_05120"
     CDS             5725..6147
                     /locus_tag="PLB33_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOG62938.1"
                     /translation="MVTADTFKRGFKNGISTLIDLSKVLVPVYIGVELLAASGFLNIL
                     AEVFKPVMSTLGLPGEASLIIILGYFLGTYAGLGAIAAMELNTVQITTIAIMLSISHS
                     LISETAIVKKLGASAAASITVRVFFSFFMGFIYYRIFG"
     gene            6152..6619
                     /locus_tag="PLB33_05125"
     CDS             6152..6619
                     /locus_tag="PLB33_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOG62939.1"
                     /translation="MSGIMDILINLLSFLWNITKVLIPIMIVIEIFKDAKLIDKISDF
                     FYPVTKFFTLSKISGISLIFGMFFGLTLGAGAILQSVKDYNVDKRSVFLVAVFLSPCH
                     AIFEDAFIMKAAGSNILPLLAARFISAIAATYIFSRAVKNNTTDVVNISNHEK"
     gene            complement(6701..>7090)
                     /locus_tag="PLB33_05130"
     CDS             complement(6701..>7090)
                     /locus_tag="PLB33_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOG62940.1"
                     /translation="YTGSIFEIAYKDLGYSIAGGGRYDEMVGNFIGEKIPAIGFSIGF
                     ERLVNQLMEENFKVPDLEKVVLLYEPSDRYIDVLAKAESIRKQGYIVSVYEKSKKLGK
                     QLNQFQGYGYKKFAVYEGDETEIKELN"
     assembly_gap    7091..7190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7191..7663)
                     /locus_tag="PLB33_05135"
     CDS             complement(<7191..7663)
                     /locus_tag="PLB33_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOG62941.1"
                     /translation="MKINLNPAKGMRDITPREKEIRDYVEGVIVDTYKKSGFELFETP
                     VVENIENLTGSQGGENLMLIFKILKRGDKLDLNKEGLTEDDLSDLGLRYDLTVPLSRF
                     YCNNRSNLLPVFKALQVGNVFRAERAQKGRYRSFKQCDIDIIGDGTQAAELELIAT"
     gene            complement(7885..9204)
                     /gene="dsdA"
                     /locus_tag="PLB33_05140"
     CDS             complement(7885..9204)
                     /gene="dsdA"
                     /locus_tag="PLB33_05140"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HOG62942.1"
                     /translation="MKEEILTKYPSLKNVSELKEVFWLNPKMVPYEEAASKINIDIAA
                     IDDAEMRLKKFAPLIEKVFPETLPSHGIIESPITEINNMKNKLQELYKLKISGKLLLK
                     MDSHLAIAGSVKARGGIYEVLKYAEALAMKHGLLKEDDDYSILAEQKYRDFFGKYKIQ
                     VGSTGNLGLSIGITSAALGFNVTVHMSADAKQWKKDLLKSKGAKVVEYDSDFSKAVEE
                     GRKQSDQDPMSYFVDDENSMNLFLGYSVAARRLKEQLNNMNIVIDETHPLFVYLPCGV
                     GGAPGGITYGLKSVYRDNVHCFFVEPTHAPSMLIGMATGLHNDISVQDLGIDGKTHAD
                     GLAVGRPSKLVGKMMDYILGGIFTIYDYKLYDYMRYLHETENISIEPSACAAFEGVKL
                     ENTEEGKQYIRKHNLENKMQNAVHIAWATGGSLVPEEINKQFLATRL"
     gene            complement(9422..9634)
                     /locus_tag="PLB33_05145"
     CDS             complement(9422..9634)
                     /locus_tag="PLB33_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HOG62943.1"
                     /translation="MAKKLVMSFLTAEGATSSMTIDAPKDDLTEAEVRAVMELIITQN
                     VFNTSKGDLAEIKSAQIISTTEEVLI"
     gene            complement(9670..9897)
                     /locus_tag="PLB33_05150"
     CDS             complement(9670..9897)
                     /locus_tag="PLB33_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HOG62944.1"
                     /translation="MPIIANQANSKVKLVLNAGLDVNNKTIMKSKTFANVKSAAQNED
                     LYNLGVAISDLQEYELANIVRYDEYELLEEI"
     gene            10011..11816
                     /locus_tag="PLB33_05155"
     CDS             10011..11816
                     /locus_tag="PLB33_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOG62945.1"
                     /translation="MDKSLISEIKCIIDEIEGYDYISKVKYVILLICNELYNSINENP
                     MKSTAIFKEYAKFDLENFKTEERIAELMGINAGNIEISSFYPASLYEALLTDKEKKSL
                     GQVYTPFEIINNMLHEVFKVKNIGVNTKILDPSCGGGYFLIEIYKYIRHNHPEIHNRH
                     IVENMLFGIDIDDFSIFLTKMGLIFHTGLDDIKFNIFNLDFLIDNSNIGKFDIIIGNP
                     PYVGHKNSTGTYKKLLYEKYSDVYYDKADISYCFFKKGKELLNLEGIISFITSRYFME
                     ALYADRLRAFIKKNYNIVALIDYSGDTAFKRAMISPAVIILSNKWNKNMFLYVKKDRD
                     VIESYKYRQDKLKSSGWIILKDEDEKLFEKIDSISNTYIKDILSIKQGIITGCDKAFI
                     VTEEVIENYKIESFLLKKWIKNSNISKKSIKYNNLYLIYSDNIQNESDCPNAISYLSK
                     YKDKLMSRRECKKGFRKWYELQWGRNKSDYENPKIIFPYKSKQNNFYFDEEAFFCSAD
                     IYLMNEFSKGLSLNYLLSYLNSEIFEFYLKCQLKKVGRNIYEYYPYKLNNLKVYLPQG
                     DIAQKFSRMENISIEIFKKLFNINDKEVNIIKNYI"
     gene            11834..13453
                     /locus_tag="PLB33_05160"
     CDS             11834..13453
                     /locus_tag="PLB33_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG62946.1"
                     /translation="MRKFKKLLLLIIIFIVLSIKPALARTVFYDTFNHWAESDINFAS
                     NTINVFKGYGDFTFRPENDITRAEFITILSRTAYKYNLMKEIYTGDLSYRDMSMKHWS
                     YTFIISMNEYLKTNKFTLQDIFPGSNFYPDKAITREESAALIAAFCKDAIYDNPLRFS
                     DVTTGNKYYNEIQRLCNSGILVGYEDNTFRGNTNITRAEAASLIKRVYSDIKTSTNDN
                     YLTKLEFMPIKGEEMYSYFGNYDLSTTNANDKKFIKAKDTLEYVAFGGYIFPEDSNLY
                     DLNAVKTMESLHASGYFNVAGTNYYMIMFGSYSSEDKSKFANEILTNIIARNDLNDAE
                     LMQIFTLVSKYDVSEALYMGALEKWNNLTSNDNSKANILFFRYAYYIKNDNKDILRAL
                     VYEDLKKNNNIVSLLNISFNSNDTNAIDFRNYNFGNYSFSLYKDTTLFRYLRNMTMPL
                     NTNLRVVELSNLLMIEKNPKPTVNMADYENLFTKYSVNKLYVLNFLGEKERAFVEGIN
                     DYDIIKTFEMYKKNPSIFDDTYTGILKKVKE"
     gene            complement(13508..14716)
                     /gene="pepT"
                     /locus_tag="PLB33_05165"
     CDS             complement(13508..14716)
                     /gene="pepT"
                     /locus_tag="PLB33_05165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOG62947.1"
                     /translation="MKVHERFLNYVKIDTQSVHDAKKIPSSEKQKDLGRLLVEEMISI
                     GIEDAYMDENGYVYGTVKGNTDAPVIGFIAHMDTSPDMPGSGVKPKIIYNYDGSDIML
                     NDEKQIVMKTEMFEHLSKYIDQDLIVTDGTTLLGADDKAGIAEIMSMAEYLINNPQIK
                     HGTIKIGFTPDEEVGQGAELFNIEKFGADYAYTVDGGEIGELEYENFNAASAKVTING
                     VNIHPGTAKNKMKNSILIGMEFHNMLPVYETPENTEGYEGFSLLTDMSGNVEKTLLEY
                     IIRDHDIAKFNIKKERFQKIADYLNEKYGQGTIELKLEDSYFNMKEKILPHMHIVENA
                     IRAMENLSIEPKIVPIRGGTDGARLSYMGLPCPNLCTGGHNFHGRYEYISIQSMEKVV
                     ELLIEIATVK"
     gene            15027..15662
                     /locus_tag="PLB33_05170"
     CDS             15027..15662
                     /locus_tag="PLB33_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOG62948.1"
                     /translation="MDFIKEGYKVKSATVIKNLEKRNMEGYYCETVEEAVEKAMSMIK
                     QDDTVGWGGSTTIDQIGIKKLLEEKNIAVYDRDKETDPAEKVKMMKKALTSDVFLTSA
                     NAITMDGELLNIDANGNRLAAYCYGPNSVIVVAGMNKIVTDLDVALKKARLDATVPNT
                     FRTNSKAPCHFTGKCIECTMSDTLCGQILITRFCKPKNRIKVILVGENLGF"
     gene            complement(15950..>16056)
                     /locus_tag="PLB33_05175"
     CDS             complement(15950..>16056)
                     /locus_tag="PLB33_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG62949.1"
                     /translation="TKTDRMDRPVSEVKLSKVTVDTFGETYEEPEIII"
BASE COUNT         4788 a         2842 c         2627 g         5599 t
ORIGIN      
        1 ctcccattga atttataaaa ttggctaagt caactatttc aggttcttct gccgcgtttt
       61 ccaagattgt ttcaccttct gcaagggttg ctgccataat tatgttttct gttgctccaa
      121 cactgggaaa atcgagatag atatctcttc caattaattt ttctgctgtt gcatggatgt
      181 atccgctttc tgatttaact tcggagccga ggtatttaaa tccttttaaa tgcaaatcaa
      241 taggccttgt ccctatggca cagccaccgg gcatacatac ctttgcattg tgacaccttg
      301 ttaataaggg gcccattacc aaaaaggatg ctctcatttt gcttatgagc tcatatggtg
      361 cttcgcaggt tttaatgttc tcagcagtta tttttaatga attcatccct tctttttcaa
      421 cctttgcacc taattcttcc agaagtttgc acattatttt aacatcctgc aaatcaggaa
      481 tgtcgtgaat tatgcattct tccttacata aaagcgttgc tgccaatatg ggcagtatag
      541 aattttttga gccgtcaact cttatgtttc ctttaagtcc ggggctcttt tttacaatta
      601 ttttttccat cattcacctt aatcaattat gtaataggaa cacactataa gataagttta
      661 gtctgataat gaagtgatat ccccaaattt attatcgtaa tagacttaac ttggtttata
      721 ataacactta tcgacatatt tttcaattgt aaatttttca tatttattta acccatatta
      781 acgtaaaaaa agaaagtgcg tatgattaaa ataaaggggg acgttatatt taaatgttac
      841 acgcgctttc tttttttata ttatgtgata aattaatttt tttatacaat aattttataa
      901 aatattattt tttgttaaaa aagaaagtct tcgatctata gcccttagaa ttgtaacgtt
      961 ttaccacacc aagataaacg agtatgctct gtgattgtca tcctgagcgt aagcgaagga
     1021 tctcaggtct cataagatga gattcttcga gctaaagctc tcagaatgac acgcagctta
     1081 ttcatagcaa tgacaagaat acggaaataa aatttactat gattagactt agaatttatt
     1141 caggcggatt tctgctttga ataagtctcc gtcaatgctc agttcaaggc ttccttcctg
     1201 tacttccatt aggcttttgg caattgccag ccctaatccg cttccctctg agcttcgtga
     1261 ttcatctccc cttttaaatc tttccataag ttcatttacg ggaatattca gttcagttga
     1321 agatatattt ttaaaactta tcgtaacatt gtcttcatct tctgaaattt caatataaac
     1381 ccttgaacct ttcagagtat atttgaaaat attcgataga agattttcca ttaccctgct
     1441 taggagccgc ccgtctgcca ttgcatatat tttttcgtta gtggctgaaa ctttaaaaat
     1501 taagtttgat tcgcttattt tctcgtccat ttcgcctaac agctgttcaa tcagggaatt
     1561 aatatgaacc ctttcaaatt tcggctcaat gctgccgctg gttgcttttg ccgcttcgaa
     1621 taaatcttcg gtaagatttt taagtctgtt ggacttcctt tccaatactt ctaagtattt
     1681 gggagcgtta tctgaattca agccttctct tttcaacaaa tcaacatatg atattatgga
     1741 tgtaagcggg gttttaatat cgtgggatac atttgtaatc agttcggttt ttagtctttc
     1801 gcttcgtact tcattttcaa ccgctgtttt tagccctgaa gttaaatcgt tgatatcttc
     1861 agaaagttgt ctgaattcac ctttcccttc taatctaatc ttaaaatcgt aatcaccgct
     1921 ttttgctacc tgcagtcctt cttgtatttt gtggaaatgt gttactttat tatagatgat
     1981 atatgttgaa ataaataaaa ttccggcaat caataagggt ttatccgctg catagacgga
     2041 agcaaaaata accaatatcg ctgctcccat tgttttatac attagcggac cagatgctgc
     2101 catttgtatt aatacgtcaa ctaattttga taaaattata taaattaccg aatgctttaa
     2161 aatagttctt tttttaatgt gccttaccag agaaagtacc aatatcagta taagtgctga
     2221 aacagctgct gtcgaaatga gcattacgag tctcattaaa ttttcgctga tagttttaat
     2281 atttaatact ttgtaaaatt ccagcctgca gactgctgca attcctgtaa tgagcagtaa
     2341 atttatatcc gtatacaggt tgtcaaatga tgtaagatga atttcatcgc ttcctgccat
     2401 ttttcctgct gatgatataa gatatgaaaa attagctata accaaaagaa tgcaaataac
     2461 tattacgccg gtatttctta tcaatacctg cctgtctgaa ttccatctgt ttgctctgtg
     2521 taaaagtgca tcatcttcta tggctgcata aatccgcata tttttattaa aggtttccga
     2581 ttcaaatata tcggtcaacg ggtcggtaac accgcctccg ttttcgggac tgagttcaac
     2641 acccttctcg cttaaaacaa tataagcatt ccgaagtaaa taaaattctt tgtttggatt
     2701 gcctgtattt gttatttcat tttcaccgtc tgttgcataa taaatataac cttggggcag
     2761 attcagctca ctgatgattt tttcatagtt tgccaaatcc gaacttatta ttgattcttt
     2821 tatttcttga atttcctgct gcttttcaat gaaaaataat tcttttgttt ctgaattatc
     2881 atcgtaatta ttttccgcga gatagttatt atacagattg gcaagtttcc aattatcctc
     2941 aatctctaat ttttcaacgg ttcctccgct taagacatat tcttcgtttt tatatactct
     3001 caatataaat tcaagacgat ttacggcata ttttaggtca ttgcttatgg tgtcgctctc
     3061 taaatagttc ttaacagcaa tgctctcata atttttgacc tttaaataaa tattcaaagc
     3121 tgaagaaact cctatcataa gaaagactat caaaagtata aatgccgaaa attttgttat
     3181 atattttttt ttaaatatct tccactttgt aaccaacacc ccacaccacc ttaaggtatc
     3241 ttggattttt aggatcaatt tcaatctttt ctcttattcg tcttatgtgg acggccactg
     3301 tgttttcagg attgaaagaa ggctcgttcc acacattttc atatatttcg tctatggaat
     3361 aaacaactcc tttgtttttg gttaacaaaa gtaatatttt gtattcaact ggtgtaagct
     3421 ttacaatttc tccgtctacc gtaacttctt ttctctcatc gtcaatcatc aaacttcctg
     3481 ttttatacag tccgaccttt tccgcatcca aagctccgcc taatgccgta taccttctaa
     3541 ggtgggattt cacccttgca ataagctcca aaggattgaa tggcttagtt atataatcat
     3601 ccgctcccat attaagccct aatactatgt ctgcttcttc ggattttgct gacagcataa
     3661 taacagggat atttttaatt tccctgattt ttgttgttgc tgttatgccg tccatttccg
     3721 gcatcattat atccattatt attaaatgca cttcattttt ttcaacaatt tcaattgcct
     3781 gttttccaga atagcatttt aaaatattgt atccttcact ttttaaatat gcttcaattg
     3841 catctactat ttctttatcg tcgtcgcaaa ccattatgtt gttcatctta tccctcctgt
     3901 atttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttata acggattctt
     4021 ccaagtcaat tctgtaattt ctcgatactg ttttttcttc cttgttcggg tcatagtctt
     4081 gaccattcca tagaactgcc aattttatac ggtcatattt accaatcata ttaaacatat
     4141 cttcaaacca ctgagcttta tctcctcctg cagaagcaca gctgaattcg gttatcatca
     4201 tgggatgagt aaatctatct atatagtcaa aataaaaatg gtcatatgct tcggagaaac
     4261 tcctccaaat ttctcctctg taataattgc ctgtattgta tgatgtcaag ccgacaatat
     4321 caacatattt atttccggga tagtagcaca aatagttgtt gtatgaaaag tcagggaatg
     4381 atttttcgtt cggattccaa acccatataa gattatcaac accgtgctct tcaaatttat
     4441 catagatata tctccagcag tctataaata aatccgtatc ctttcctact ttatgggagc
     4501 agtataaaac ccattcgccg ttcatttcat tgtttagtct gaacaacacg ggataatcat
     4561 attcattaaa gctttgagct aagttatcta aataatcatc gtactttcct tccaatatgt
     4621 ctagggttat atctttttcc cttccattta ttatgtcaat cgtatataag ccgtattcca
     4681 caaccttccc taattccttc gctttattca tataatctgt tttaaaaggc agggaaaaat
     4741 tgttgtacag caatgctacc ggaaaattat aatcaagcat tttttctatt tgcaaaagcc
     4801 tgtatgcata taaaggaaat gtaggctcaa aaataccgaa agctgttttt tcgttgttaa
     4861 tgaagtattt atcgtaaaag tcccttgttt tttcatcaaa attctttggc tcgggatgaa
     4921 agactttatc atttttcaaa gtttcagtct tagccgtaaa tttaaattcc ggcataatat
     4981 catcaatatt tatgggtgct gctgatttta taaatatggt tatggattca tatgtatttt
     5041 tttcaaatgt aattgttgcg taataattcc tgtccggttc cttggttttt acctttctcc
     5101 tttcgaacag tgttacatgc cctgatagtc cgccaatact atgattgtat tcatctgtaa
     5161 ttttaaattc aggatttttt aatatgcctc tgttgcagta gtttcgatgt gtagcatgtg
     5221 aatttatcgt attgtaaaaa ttatcataaa aaacttccac taccaggtca tccgattcaa
     5281 atcggctttt tacgcttact atatcttcat taagcgtaag attatttgga attgttattt
     5341 catacccgta ggcactattt atatacttat tgtactctgg agaatatttt attaactctg
     5401 tacttttatc aatttccgga agctgtggaa tctccggata cgttgcatcg tttgtatcgt
     5461 caggttgaag atattctatt gtgttggtaa atgtttcttc tgcattgaca ggtgtaaatg
     5521 taagtgcaaa aattagtccg attaatattt ctctcatgta attctccttg gtcttattca
     5581 ttcttattaa ttttatcaaa gatagcaatt atagtcaaac tcaattgtgt cacttccctt
     5641 attaaccgta acgtttagaa tcgcagaagt tttgattgtt aataaataat ttgtatgcta
     5701 taatttttct gttaggaggt agcaatggtt actgcagaca cttttaaaag aggttttaaa
     5761 aacggtattt ctactcttat agatttatcg aaagtattgg ttcccgtgta cataggagtt
     5821 gaactgcttg ctgcatcagg ttttttaaat atattagctg aagtatttaa gcctgtaatg
     5881 tcaacattgg gactgcccgg tgaagcttca ctgataataa tattaggtta ttttttggga
     5941 acatatgccg gcttgggtgc aattgctgcc atggaactta atacagtaca aataacaaca
     6001 atagcaatta tgctttcaat atcccacagt ctgatttcag aaacggcaat agtaaaaaaa
     6061 ttaggtgcaa gtgcagcagc cagtattaca gtaagggtgt ttttttcatt ttttatggga
     6121 tttatatatt ataggatatt tgggtgatat tatgagtgga attatggata tattgataaa
     6181 tttattgagc tttttatgga atataactaa ggtactgatt ccaattatga ttgtaattga
     6241 aatatttaag gatgcaaaat taattgataa aatatctgac tttttttatc ctgtcacaaa
     6301 attttttaca ttaagcaaga tatccggaat atcccttata ttcggtatgt ttttcggact
     6361 gacacttggc gcgggtgcca tccttcaaag tgttaaggac tacaatgtag acaaaagaag
     6421 tgttttttta gtagccgtgt ttttgtcccc gtgtcatgca atatttgaag atgcatttat
     6481 aatgaaagct gcaggctcaa atattttgcc cttgttagca gcaagattta tttctgcaat
     6541 tgctgcaaca tatatatttt cacgagctgt aaaaaacaat acgactgatg ttgtaaatat
     6601 aagcaaccat gaaaaatgag attcttcggg ctgcgccctc agaatgacga ttgataggtc
     6661 attcagtgct gcaggtgaag aatctcatag tatctattct ttaatttaat tcttttattt
     6721 cggtttcgtc tccttcgtaa acggcgaatt ttttgtatcc gtatccttgg aattgattta
     6781 attgcttgcc taatttcttg gatttctcat atactgatac gatatagcct tgttttctta
     6841 tactttcagc ttttgcaaga acatctatgt atctgtccga tggctcatat aatagcacta
     6901 ccttttccaa atcaggaact ttgaagtttt cttccatcag ctggtttacc agcctttcaa
     6961 atcctattga aaagcctatg gcaggtatct tttcgcctat gaaatttcct accatttcgt
     7021 cgtatctccc gccgccggct attgaatagc ctaaatcttt atatgcaatt tcaaatattg
     7081 agccagtgta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttgcaatca
     7201 gttctaattc cgctgcctgc gtaccgtctc ctattatatc tatatcgcat tgtttaaagc
     7261 ttctgtatct tcctttttga gccctttcag ccctgaacac attccccacc tgcagagctt
     7321 taaatacagg gagtaaatta gagcggttat tgcaataaaa acggcttaaa ggaactgtca
     7381 aatcgtatct tagtcctaaa tcggacaggt catcttctgt taatccttcc ttatttaaat
     7441 caagtttatc tcctcttttt aatattttaa aaataagcat taaattttct ccgccttggc
     7501 tgcctgttaa attttctata ttttcaacaa caggtgtttc aaacagctcg aaaccgcttt
     7561 ttttatacgt atcgactatt accccttcca catagtctct aatttccttt tctcttggcg
     7621 ttatatctct cattcccttt gcagggttta aattaatttt catccgttct gcctcctacc
     7681 ttatgggaca gtggggacgt tcctgactgt cccacttttt gtccctaatt ctaattttat
     7741 atattgttaa ctattttacc aaatagcagt ttaaatatca aatttttttg aaaaatgaaa
     7801 tacttgctgc gttcagatga cagtacctcc atccatttgg ccatacactc atgatgacag
     7861 tacccccgtc cccttgtcat attgtcatag gcgggttgcg aggaactgct tgttaatttc
     7921 ttccggcacg aggcttccgc ctgttgccca agctatatgt accgcatttt gcattttgtt
     7981 ttcaaggttg tgctttctta tgtattgttt tccttcttcc gtgttttcga gtttgacacc
     8041 ttcaaatgca gcgcatgctg agggctcaat actgatattc tctgtttcat ggagatatcg
     8101 catataatcg tacagcttat aatcatatat tgtaaatatt cctcccaaaa tatagtccat
     8161 cattttcccg accaattttg aaggtcttcc tacagccaat ccgtctgcat gagtttttcc
     8221 gtctatcccc aaatcctgca cggaaatgtc attgtgaagc cctgtagcca tacctatgag
     8281 catgcttggc gcatgagtcg gctcgacgaa aaagcagtga acattgtccc tatagactga
     8341 tttaagtcca taggtaattc ctccaggtgc tcccccaaca ccgcagggaa ggtaaacgaa
     8401 caaaggatgt gtctcatcaa taacaatgtt catgttgttt aactgctcct tcagccttct
     8461 tgctgcaaca ctgtagccta aaaataaatt cattgagttt tcatcatcta caaaataact
     8521 catagggtct tggtctgatt gttttcgtcc ttcttccact gctttagaaa aatcgctgtc
     8581 atattccacg acctttgctc cttttgattt aagcaggtcc tttttccatt gctttgcatc
     8641 cgccgacatg tggactgtaa cattaaatcc gagggctgcc gatgtaatgc ctatacttaa
     8701 gcctaaattt ccggtggagc cgacctgaat tttatacttg ccaaaaaagt ctctgtattt
     8761 ctgctctgcc aaaattgaat aatcatcatc ttcttttaac aatccatgtt tcattgccaa
     8821 tgcttcagca tactttaata cttcatatat gccgcctctt gcctttacgc ttcctgcaat
     8881 tgccagatga ctgtccattt taagcagcag ttttccggaa attttcagtt tataaagttc
     8941 ttgaagcttg tttttcatat tgttgatttc tgttatggga gattcaatta tgccatgaga
     9001 tggaagagtt tcaggaaata ctttttcaat cagcggcgca aattttttaa gcctcatttc
     9061 agcatcgtca attgctgcaa tatcaatatt tattttagat gcagcctctt cataaggaac
     9121 cattttgggg tttagccaga atacttcttt caattcagat acatttttaa gacttggata
     9181 tttagttaga atttcttctt tcatatttcc tccttagtat attctatatg taatgtcatc
     9241 ctgagtgcag cggaatttca ggttttgaga ttcttcgggc aaactctcag aatgacaact
     9301 tagtttttga cagggggaca tacctaagag gaatgtcccc ggttgtattc attgtatcgg
     9361 tgcaaagacc agagtctttg cttcattgat tgtattcatt ataacataat aaattaacta
     9421 attatattaa aacttcttcg gtagttgaaa ttatttgagc tgattttatt tcagccaagt
     9481 cgcccttgga agtgttgaaa acattttgtg ttatgattaa ctccatgact gctctcactt
     9541 ctgcctctgt caaatcatcc tttggtgcat caatcgtcat ggatgaagtt gctccctcag
     9601 ctgtcaagaa agacatcact aactttttag ccattatatc acccctttcc gtttatgatt
     9661 tatattaact tatatttctt ctagcaattc gtactcatcg tatcttacaa tgtttgcaag
     9721 ctcgtattcc tgaaggtctg aaatagcaac tccaagattg tacaggtctt cattttgcgc
     9781 agctgatttc acgttagcaa atgtcttgct cttcatgata gtcttgttgt ttacgtcaag
     9841 tcccgcgtta agaaccaatt taactttaga atttgcttga tttgcaatta tcggcattat
     9901 atcacctccc gtttaattct gtaactatat agcatttata agcaggatgt attgcttaaa
     9961 tatcaaaacc atgctagtat atacataatg atgcaattca aggggtagtt atggacaagt
    10021 cgcttataag tgaaataaag tgcataattg atgaaattga aggatatgat tacattagca
    10081 aggtaaagta tgttatttta ttgatatgca atgaattgta taattcgata aatgaaaacc
    10141 ctatgaaaag cacggcgatt ttcaaggaat atgctaagtt tgacttggaa aactttaaga
    10201 ctgaagaaag aattgccgaa cttatgggga taaatgccgg taacattgaa atttcaagct
    10261 tctatcctgc ctccttgtat gaagctctgc ttactgacaa ggaaaaaaaa tccctcggtc
    10321 aggtttatac tccatttgaa ataataaata atatgctgca tgaagtgttt aaggttaaaa
    10381 atataggtgt aaatactaaa atattagacc catcctgcgg cggaggttat tttttaattg
    10441 agatttataa atatataagg cataaccatc ctgaaatcca taatagacat atcgttgaaa
    10501 atatgctctt tggaattgat attgatgatt tttcgatatt tttaactaaa atgggactta
    10561 tttttcacac cggactggat gatattaaat tcaatatatt caatttggat tttctgatag
    10621 ataattcaaa tataggcaaa tttgatatta taatcggaaa tcctccatat gttggtcata
    10681 aaaattcgac aggcacatat aaaaagcttt tgtatgaaaa gtattcagat gtttattacg
    10741 ataaggctga tatttcctac tgctttttca aaaaagggaa ggaattgctg aatctggaag
    10801 gtattatttc ctttattact tcccgttatt ttatggaagc attatatgca gacagactga
    10861 gggcttttat aaaaaaaaat tacaatattg tcgcactgat tgactacagc ggtgacacag
    10921 cctttaaaag ggctatgata agtcctgcgg taataatact ttctaataaa tggaacaaaa
    10981 acatgttttt atacgtcaaa aaagacaggg atgttatcga atcatataaa tatagacaag
    11041 acaagctaaa gagctcaggg tggattatat taaaggatga ggatgaaaag ctttttgaga
    11101 aaattgacag catttcaaac acatatatca aggatatatt aagcattaag cagggaatta
    11161 ttacgggctg tgataaagct tttatagtaa ccgaggaagt tatcgaaaat tacaaaattg
    11221 agagttttct actcaaaaaa tggattaaaa acagcaacat atcaaagaaa tcgataaaat
    11281 ataataatct atacctgata tattccgata atatacagaa tgagagcgat tgtcccaatg
    11341 caataagtta tctgtccaaa tataaggata aacttatgag cagaagagaa tgcaaaaagg
    11401 gatttcgcaa atggtatgag ctgcaatggg gcagaaataa atctgactac gaaaatccta
    11461 aaataatatt cccgtataaa tcaaaacaaa ataattttta ttttgatgaa gaagcatttt
    11521 tttgcagtgc tgatatttat ttaatgaatg aattttcaaa gggattgtct ttaaattatc
    11581 tgctttctta tttaaattct gaaattttcg agttttattt gaaatgccag cttaagaagg
    11641 taggaagaaa tatttatgaa tattatccct ataaattaaa taatttgaag gtctatttac
    11701 ctcagggaga catagctcaa aaattttcac gtatggagaa tattagcatt gaaatattta
    11761 aaaaattgtt taatattaat gataaggagg taaatataat taagaactat atatgaagaa
    11821 ggtgatgacg caattgagga aatttaaaaa gctattatta ttaattatta tttttattgt
    11881 gttgtccata aagcctgctt tagcaagaac ggtattctac gatactttca atcattgggc
    11941 ggaaagtgac ataaattttg cctccaatac tattaatgtt tttaagggat atggagattt
    12001 cactttcaga ccggaaaatg atataacaag agcagaattt ataaccatac tatcaagaac
    12061 tgcatataag tataatctta tgaaagaaat ctataccggc gatttatcat acagggatat
    12121 gtccatgaag cattggtctt atacttttat tatttctatg aatgaatatt taaaaacaaa
    12181 caaatttact ttacaggaca tcttcccggg cagtaatttt tatcctgaca aagctatcac
    12241 cagagaggaa tcagccgcct tgattgctgc attctgcaag gatgcaattt atgacaatcc
    12301 tcttaggttc agcgatgtga ctaccggtaa caaatattat aatgaaatac aaagactatg
    12361 caattcagga atacttgtcg gttatgaaga caatacattc agaggcaata ctaacattac
    12421 cagagcagaa gcagcttctt taataaagag agtttattcg gatataaaaa caagcactaa
    12481 cgataattat cttactaaac ttgaatttat gcctataaaa ggcgaagaga tgtattctta
    12541 ttttggcaat tatgatttaa gcactacaaa tgctaatgac aaaaaattta ttaaggctaa
    12601 agacactctg gaatatgttg catttggcgg ttatatcttt ccggaggatt caaaccttta
    12661 tgatttaaat gcagttaaaa caatggaatc cttgcatgca agcggatatt tcaatgtagc
    12721 aggcactaat tattacatga ttatgttcgg cagctattcc agcgaagata aatcaaaatt
    12781 tgccaatgaa attttgacaa atataatagc tagaaatgat ttaaacgatg cggagttaat
    12841 gcagattttt acattggtaa gcaaatatga cgtaagtgaa gctttgtaca tgggagcact
    12901 tgaaaaatgg aacaacttaa cgagcaatga taattcaaag gctaatattt tatttttcag
    12961 atatgcttat tatattaaaa acgacaacaa agatattctt agagctctgg tttatgagga
    13021 tttgaaaaaa aacaacaata ttgtttctct tttgaatatc agttttaatt caaacgatac
    13081 aaacgcaatt gattttagga attataattt tggaaattat agtttttcat tgtataagga
    13141 tacaacctta ttcagatact taagaaatat gactatgcca ttaaatacta acttaagagt
    13201 tgtcgaactg tcaaatttgc tgatgattga aaaaaacccg aaaccgacag taaacatggc
    13261 tgattatgaa aatctgttta ccaaatattc cgttaacaaa ttgtatgttc ttaatttctt
    13321 gggtgaaaag gaaagagcat ttgttgaggg aattaacgat tatgatatta tcaaaacttt
    13381 tgaaatgtac aagaaaaatc cctcaatatt tgacgacacc tacacaggga ttttgaaaaa
    13441 agtaaaagaa taagtcatag aagtttggtg ccaggcaccg gtaagtggtg cctggcacca
    13501 agtgtctcta tttcactgtt gcaatttcta tcaacagttc cacaaccttt tccattgact
    13561 gaattgatat gtattcgtat ctaccgtgaa aattatgacc tccggtgcat agattcggac
    13621 aaggtaaacc catgtatgac aatctagctc cgtcggttcc acctcttatt ggaactatct
    13681 ttggctcaat acttaaattt tccatagccc taattgcatt ttccacaatg tgcatgtgag
    13741 ggagaatctt ctccttcata ttaaaatatg aatcttctaa ttttaactct atcgttcctt
    13801 gaccgtattt ttcgtttaag tagtctgcaa tcttttgaaa tctttccttt tttatattga
    13861 attttgcaat atcgtggtcg cgaataatat attcgagcag tgttttttca acatttccgg
    13921 acatatcggt taagaggctg aatccttcgt atccttcggt attctccgga gtttcatata
    13981 caggcagcat gttgtgaaat tccattccga taagtatgga gtttttcatt ttgtttttgg
    14041 cagttcccgg atgaatattc actccgttta ttgttacttt tgcacttgca gcattgaagt
    14101 tttcatactc caattctcct atttctcctc cgtcaacagt atatgcataa tcagccccaa
    14161 atttttcaat attgaacagc tctgccccct gaccaacttc ctcatcggga gtgaatccta
    14221 tttttattgt accgtgttta atttgtggat tgttgattaa gtattcagcc attgacatta
    14281 tttcagcaat tcctgccttg tcatcggcac ctagaagggt tgtgccgtct gttacaatca
    14341 aatcctgatc tatatattta gataaatgct caaacatttc agttttcatt acaatttgtt
    14401 tttcatcatt tagcattatg tcgctgccgt cataattata tattatctta ggttttacac
    14461 cgctcccggg catatcgggc gatgtgtcca tatgagcaat aaatcctata acaggtgcat
    14521 ctgtattacc tttaacagtt ccgtaaacgt atccgttttc atccatgtat gcatcttcga
    14581 ttcctattga tatcatttct tcaaccagca gtcttcctaa gtccttttgt ttttcactgc
    14641 ttggaatttt ttttgcatcg tgtacggatt gagtatcaat tttaacataa tttaaaaatc
    14701 tttcgtgaac tttcattttg tcctcgcttt attaattatt ttatgatatt agtatatcat
    14761 tatggatttt atatatcaat attctttatg ataagaacat acctgagggt gcagcctggc
    14821 attctgagag ctttagctcg aagaatctca tcttataaaa cctgagatcc ttcgcttacg
    14881 ctcaggatga caatcgcaga gtatattcgt ttatagtggt gtggtaatca catgataatt
    14941 ctgaaagctc tcgctcgaag aatctaattg ttgaattgaa gattaaaata atatataata
    15001 tggacaagaa tatatgggag gttgttatgg attttattaa agaaggatat aaggtaaaaa
    15061 gtgctactgt tattaagaat cttgaaaaaa gaaatatgga aggttactat tgtgaaacag
    15121 tagaagaagc tgtagaaaaa gcaatgtcaa tgattaaaca ggatgataca gttggatggg
    15181 gcggttcaac tactatagac caaattggaa tcaagaaact tttggaagaa aaaaatattg
    15241 ctgtctatga tagggataaa gaaactgacc ctgcagaaaa agtaaaaatg atgaaaaagg
    15301 cattgacttc tgatgtgttc cttacaagtg ccaatgctat tacaatggac ggagaacttc
    15361 taaatattga tgcaaacggt aatcggcttg ctgcatattg ctacggtccc aacagcgtta
    15421 tagttgttgc aggaatgaac aaaatagtaa cagatttgga tgttgcgctg aaaaaagcaa
    15481 gacttgatgc aactgttcca aacaccttca gaacaaactc aaaagcccca tgccacttta
    15541 cgggcaaatg tatagagtgc acgatgagcg atacattgtg cggtcaaatt cttataacaa
    15601 gattctgcaa gcctaaaaat agaataaaag taatattggt tggtgaaaat ttagggtttt
    15661 aagatgccct aaccctattt ttcagagtct tttttctgaa aaatagcggt aggtcattcg
    15721 caacgaattc ccaatttata cgaccaaagg gagtaaataa attggtgaat gagactgcga
    15781 ggtatgggga catactcctt agcaggagga atgtcccctt attacgaagt gttatcctga
    15841 acaaagagaa ggatctcatg agattcttcg agctaaagct ctcagaatga caggtagctt
    15901 attcatagca atggctatcc acgatttatt catataaaac aacaatagtt tatattataa
    15961 tttccggctc ttcgtatgtt tctccgaatg tatcaactgt aaccttgcta agtttaactt
    16021 ctgaaacagg tctgtccatg cggtctgttt ttgttg
//
LOCUS       DAOSHP010000037        15292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3176_length_15292_cov_9.760320, whole genome shotgun sequence.
ACCESSION   DAOSHP010000037 DAOSHP010000000
VERSION     DAOSHP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15292
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3176_length_15292_cov_9.760320"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..624
                     /locus_tag="PLB33_05180"
     CDS             28..624
                     /locus_tag="PLB33_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOG62950.1"
                     /translation="MKDPYEILGLKRGATKEEVKGAYRKLAKKYHPDMNVNNPLQDLA
                     EEKFKEIQWAYDEIMSGNADGPGYGSTYTGNNSKSTGDLFSARQYVNAGRFYEALSIL
                     RKTKVRNAEWNFLMGVCYVSLGSINQGMQHIQTAVNMEPSNMEYQNYLNQIYNMQRTY
                     QQRAYSYGGGSSSVDCCAQLICADCLCECLGGDLISCC"
     gene            615..1115
                     /locus_tag="PLB33_05185"
     CDS             615..1115
                     /locus_tag="PLB33_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62951.1"
                     /translation="MLLKSNNVAYLGVLLGLNQLFIILSSVIETNTIILMAAAALIVG
                     VVVVEFGGKAGIIFYIASCISGFFLTFNKIEIITYIYFFGIYSIIKHYIETKIFNKYI
                     SYAVKIASFNISILLMYFTAKLFINLPIRWWMIIAAQVLFIIYDYAYTIFINQYINFI
                     KPKLGR"
     gene            1339..3117
                     /locus_tag="PLB33_05190"
     CDS             1339..3117
                     /locus_tag="PLB33_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOG62952.1"
                     /translation="MLKKIKYAILLLLLTVLLTSCTESADADANTPPSDETNQEIPEI
                     IESELSEEEKAKLELEKRIAEREELEEQRKEELGEFYVPLIPIGEEREKKSVEVKALY
                     VTGHTAGNDLNKENIDAYGEYVKALAENNTQKARELFDKAEKANKFERIVGIALATEI
                     NGLVINVKDDNGFITYKSNIEIVKEMNKETPIPIKDIDNMLKILKEYDLYIIGRIVAF
                     KDKNFAVKSPEHAIQLKSGGIWLDPNSGKIPWINPFDKYVWDYNVAIAKEASLLGFDE
                     IQFDYVRFPDGAKTYNAIAEFPGRDGRDKDEAIADFLEYSKKELEPYNVNLGADVFGL
                     VTRTWDDYPEDIGQTWILIGKHVDNICPMVYPSHYSTGWYNLENPNAHPYLVVKGAME
                     EAIEKNSSMETPPIIRPWIQDFDWDGIAYGPDKVRDQIIAAKELGVYEYMIWNPSNVY
                     DPYAFMVTEKEKITAYPLNKGELDYREKSPADAAVKYLTGMLQDRHSYTYLMTPVSDR
                     VNDFDEFVKLREEDNIKLLSYKVYGYEMDANDSNKANVSLYYKIEVKNGDATEIIEKD
                     NVEWQAIRELNIWKVKVNWDIGKNVH"
     gene            3244..4257
                     /locus_tag="PLB33_05195"
     CDS             3244..4257
                     /locus_tag="PLB33_05195"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOG62953.1"
                     /translation="MKKYNVAILGATGAVGQEMLKVLGERNFPINDLKLLASKRSAGA
                     IVEFNGEEYAIEEATESSFENIDVVLCAAENDISEKLSPAAVKAGAVVVDNSSAFRLY
                     DDVPLVVPEVNGEDVKNHQGIIANPNCSTTIALTAINELNKYSPIKRMIVTTFQAVSG
                     AGVNGIKELNQQIKDISEGKDVEINTFQHQIAYNVIPQIGSFNEMGYSQEEMKLLNEG
                     RKILHNPDLKVNCTCVRVPVYRSHSESITIETEKEITVEKARELLSKAAGVKLIDDLE
                     NNKYPMPLDSSDQDLIYVGRIRKDISGENSLVLWCCGDQIRKGAATNAVQIAEVLVKE
                     NLI"
     gene            complement(4320..6062)
                     /locus_tag="PLB33_05200"
     CDS             complement(4320..6062)
                     /locus_tag="PLB33_05200"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG62954.1"
                     /translation="MKKAISLVLVIIIFFSAAQPCVLAADIETYNQLDKMVQRIVDEG
                     KSKGAVLSVVKDGNIELCKGYGLADDNLGIASDGEKIAFRIGSVSKTFVAVAAQILVE
                     KELLNMDSDISIYLDLDLKLTYPVSMRQLLTHTAGFEDMVTGMAFYNVSDTEPLEISV
                     RKYLPRQIYKPGEVISYSNYGIALAAYVIECITEQDFALFCEEKIFEPLNMNRTTFRF
                     MHDKAYVSKAYSPNGNETIEPYMNLYPEGSAVSTAEDMAKYMIWLMDGDDNRVLSPDS
                     KAELFKKQFTMSEEFEGIGLIWNRKEQDNKVYYDKKGETLNFYTRIALYSQADTGVFL
                     SFNTYVPEHEINNIMNKATDLIYGEKQEYTIKNADSSIKIGGLYVNNWSSFNTPEKIL
                     RYFIPNKMIYIDTVKDGHYINGQKMTLIDEDLYSSDLGLLKFKEVQNKIIMSSESAIT
                     YVKVPLWQHRIIQMIIPIIFIIVTFIIFIRELIKKFSNKNNKYSFAFALNSFFQIVSF
                     VILCLLMYKGIVTFSILKYAPYMKFLGWFIAALVISNIIIAIKNKKYKELLPAIWVLS
                     GILFSAWMLFFHIL"
     gene            6220..6927
                     /locus_tag="PLB33_05205"
     CDS             6220..6927
                     /locus_tag="PLB33_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG62955.1"
                     /translation="MNILVAEDEIDIRNLIKITLEDEGYSVITACDGIEALKKLKETP
                     VDLAILDIMMPRLDGFNLLRKIRENSTIPVIFLTARTDDLDKVLGLELGADDYIAKPF
                     SMSELIARVRAQLRRNNEYMLPKQENKTLLQYGDLTIDKESCCAYKNNEQIELNAKEY
                     KLLLHLMENPERVFTKKQLYNAVWDDDYYYDDNTIMVHISHLRNKIETDSQNPEYIKT
                     IRGLGYKLHKTGGYNEK"
     gene            6917..8323
                     /locus_tag="PLB33_05210"
     CDS             6917..8323
                     /locus_tag="PLB33_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG62956.1"
                     /translation="MKNKISHQFLLNYLTFFLMSLLAALFAYLLLSFADDVISKTLVK
                     NRYPSELIMKDNYADIDAESVVEHGGGVQVINENYEVVYSKGLDTLGKKQFSVSEFTD
                     FLVKSKEKGFKYHYDILYNPQAKFWLIVTFPTSMRLDISFVYNKEAASEDLKNVSFVF
                     ISVFILYLLLLGLLAFIFSKITSIHIIKPLKMLKEGTKALREGDYSARVSMNLKNEFA
                     ELQDTFNEMAEKIETEIKLREQSEAERKKIIMDISHDLKNPLAIVVGYTELCLKKAES
                     LDKELINHLQIIFKNSKRAGVLLNDLFEFSKLESSEFSMKFHKTDICEYLRQACGDIL
                     PMIEQAGFEYSFDIPEKAVYVMLDVSQMNRVLFNLAENAIRYNQSGTSLSVSLYDDKT
                     NVLIYFSDDGIGIPSHLAKNIFKPFVRIDDSRNSQTGGTGLGLSIAYKIIKSHGGDME
                     LKTDLNKGCTYIITLPKI"
     gene            8450..9673
                     /locus_tag="PLB33_05215"
     CDS             8450..9673
                     /locus_tag="PLB33_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOG62957.1"
                     /translation="MYDSVIISLLSLSLIINIFLLIKQIKNNTNERIISELKIYLKDN
                     NLDLIDSVNRKIAESEQKQLRELLTNKLETVERIDRNSNKLTESFAKFSADTSKTLNE
                     NFISLNERVSQNLDRINLRVEERLNEGFEKTNKTFVNILERLSRIDEAQKKIDSLSTN
                     IVSLQDVLTDKKSRGTFGEVQLNHILSVVFGEKNDKIYEIQKKLENSMIADAVLYIPE
                     PVGMLCIDSKFPLENYQRMIDVNISEAERKQYSRDFKLNVKMHINDISSKYIIPGQTS
                     DQAIMFIPAEAIFAEINAYHQDLLDYAGEKKVWITSPTTLMSVLSTVQVVLRNIEREK
                     YAGIIHEELNKLGKEFKLYKDRWESLSRNIKRVSDDVDKINITSNKIERKFDRISRVE
                     MGCEPEIAEIEYSEI"
     gene            9733..9815
                     /locus_tag="PLB33_05220"
     tRNA            9733..9815
                     /locus_tag="PLB33_05220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9766..9768,aa:Leu,seq:caa)
     gene            9989..10855
                     /gene="rsmI"
                     /locus_tag="PLB33_05225"
     CDS             9989..10855
                     /gene="rsmI"
                     /locus_tag="PLB33_05225"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOG62958.1"
                     /translation="MSKLYVVPTPIGNLGDMTNRAVETLNSCDLILAEDTRHTQKLLN
                     HFNIKTKMISYHKFNEKQRSEEIIHKLLNEDINAALVTDAGTPCISDPGYELVREVRE
                     KGIEVIGLTGASALTTALSISGIETYEFAFYGFLPRKKSELDTALKRVLNNPVKTFVF
                     YESPKRIISLIENIKIHMPNSTLCVCKELTKIHEKTFYGSVDDVLEQLNNHGDVEKGE
                     YVIVGYNNDYKEKGDTDIQVSLHAILFDKLFKNEYSLKEAVNEAALEGIASKNEAYKA
                     SLEIKKIIEKLR"
     gene            10861..11541
                     /locus_tag="PLB33_05230"
     CDS             10861..11541
                     /locus_tag="PLB33_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOG62959.1"
                     /translation="MIGTIANALAIIVGGIIGLLFKNIIPEKISEALLKAIGLAVIGI
                     GINLMLSGENFTLLIISMVIGTIIGEGIDIEKKLDKIGAFIESKMKNKESNVALGFVA
                     CTLVYCVGSMAIVGSIQSGLTGNHEILFSKAVLDGITAVTFAATMGVGVVFSGISVLV
                     YQGTITMLASIMQSLLNPVVVSEMTAIGGVIIMGIGLNFLISNRMRVGNMLPSIFIPI
                     IYYMILMR"
     gene            11546..12892
                     /locus_tag="PLB33_05235"
     CDS             11546..12892
                     /locus_tag="PLB33_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOG62960.1"
                     /translation="MDRNLVLKPSAIMSICLIFIIGSIILSIPLYFGFLAGIVLTSTV
                     LYKSGYHMREIYETSVSALKDVKRLLIVILFIGATTSIWLASGVVPTIMYYGFTYMEG
                     VNFLLAVFLITSVVSYFLGTAIGTISTVGISLSGIGLSIGIPHNLMVGVLVSGAFIAD
                     KISPLSGLVNLGMTTVNRNYKELFKSMIITLIPAIAATSVIYFIIGSSYIAKTENLIM
                     YKQAIQEGFNTSPALFILPAVILILSISGLDSLKTFSIGIGVGAILSIIFQQESLLSI
                     IKALIFGYRGNTPSEELNRLLLSGGMISMIEAIFIVAGAYLLIKLFEKGNVLVPLMDK
                     LVYGINNKMSLIGRTGLISILMTVATCDQTSGIILPGTILQEKYKELKLDNAVLARTI
                     FDTGVIVAPLMPWNINSFLIKPVMGITAAQYAPFAVLCIVCPLITMLVSYFQYRNS"
     gene            13115..14902
                     /locus_tag="PLB33_05240"
     CDS             13115..14902
                     /locus_tag="PLB33_05240"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOG62961.1"
                     /translation="MKKLTDNSNVLKWVDEIISMTNPSNVVWIDGSEELYEELRKEGC
                     STGELVKLNEEIYPGCYYHRSAKDDVARMEDKTFICSRKKEDAGNTNNWKSPEEAYKL
                     GREIFTDSMKGRTMYIIPYSMGPVGSPFSKIGIEITDSIYVVLSMNIMTRVGKEVLDA
                     LGDSNDFVRGVHGKCTLDINKRYIFHFPEDNTIWSCNSGYGGNVLLGKKCFALRIASY
                     QGRMEGWMAEHMLIVGIENPQGEVKYIAGAFPSACGKTNLAMLIPPEIYLNKGYKVWT
                     VGDDIAWLRIKEDGRLWAINPEAGFFGVAPGTNAKSNYNALMSTKKNSIFTNVVLNLD
                     DNTVWWEKLDNNPPENALDWMGNPWNGKTSKENGAHPNSRFTAPAKNCPCISPEWESP
                     EGVPLSAIIFGGRRAKTAPLVYQSRDWQHGTFVGSILASETTAAASGKVGVVRRDPMA
                     MLPFCGYNMGDYFKHWLEMGKKMSNPPKIFHVNWFRTNEEGQFIWPGFGDNFRVIEWM
                     LKRCENKADANEAPIGYLPKPEDINIEGLNLSADTLKSLLSIDTESWLADIKNIEEFY
                     AKIGDAIPQELIEELRTLADNLIRSAALV"
BASE COUNT         5422 a         2301 c         2986 g         4583 t
ORIGIN      
        1 tgaataaatt taataaggag aaaacaaatg aaagacccat atgaaattct tggtttgaaa
       61 agaggagcaa caaaggaaga agttaaaggt gcttatagaa agttagcaaa aaagtatcac
      121 cccgacatga atgtgaacaa tcctcttcag gatttggcgg aagaaaagtt caaggaaatt
      181 caatgggctt atgacgaaat aatgagcgga aatgcagacg gtccgggcta tggctcaaca
      241 tataccggca ataattcaaa aagcaccggt gatttgtttt ctgcaagaca atatgttaat
      301 gcagggagat tttatgaagc tttgagtata ctccgaaaaa ctaaagtcag aaatgcagag
      361 tggaattttt taatgggtgt atgctacgtg agtttaggct ccataaatca aggaatgcag
      421 catattcaaa ctgccgtgaa catggagcct tccaacatgg aatatcaaaa ttatttaaat
      481 caaatttaca atatgcagcg cacataccaa caaagggctt acagttacgg aggaggctca
      541 agttcggttg attgctgtgc tcaactgata tgcgctgatt gtctgtgtga atgtttggga
      601 ggggacctga tttcatgctg ctaaaaagca ataatgtggc atacttaggt gtgcttttag
      661 gcttaaatca gttgtttata atactttcat cggtaattga aacaaacacc ataatattaa
      721 tggcagcagc agcattgatt gtaggagttg tggtagttga atttggaggg aaagcaggaa
      781 taatatttta tattgcatca tgtatttcag gattcttttt aacattcaac aaaattgaga
      841 ttattacata catatatttt ttcggaattt acagtattat taaacactat attgaaacaa
      901 aaatattcaa taaatacata tcctatgctg taaaaatagc atcttttaac atttctatct
      961 tattaatgta ctttactgca aaactattta taaacttacc tatccgatgg tggatgataa
     1021 tagcagccca agtattgttt attatctacg actacgccta tacaatattc ataaatcaat
     1081 atataaattt tataaaacca aagttgggaa gatagaagca cttctcattt ttaagattga
     1141 attctgagaa atggttgaca tatctttcgt aaacgagcaa caattttgtg tgacgtaagg
     1201 agtcgaaaaa tgaaaaaacc tgtattatcc attgcttata ggtatagatt atgttaaaat
     1261 taatcgtggt ataagagtag tttataaata ttatatacta tgggtcatct gcacctatta
     1321 tttaaaattg gaggatttat gcttaaaaaa ataaaatatg caattttatt attattacta
     1381 accgtgttat tgacatcttg tactgaatcc gcagatgcag atgcaaatac acccccttcc
     1441 gatgaaacaa atcaggaaat tccggaaata atagaatccg aattatcaga agaagaaaaa
     1501 gcaaaattag agttggaaaa aagaattgct gaaagggaag aattagaaga acaaaggaaa
     1561 gaagagctgg gagaatttta tgttccgctg attcccatag gggaagaaag agaaaaaaag
     1621 agtgttgaag ttaaagcatt gtatgttacc ggtcatacgg caggcaatga tttaaacaag
     1681 gaaaatatcg atgcatatgg tgaatatgtg aaagcacttg ccgaaaataa cactcaaaaa
     1741 gcaagggaat tgtttgacaa ggctgaaaag gccaataaat ttgagcgtat tgtgggaata
     1801 gctcttgcta ctgagataaa cggattggtt ataaatgtta aagatgacaa cggctttata
     1861 acatataaga gcaatattga aattgtcaaa gaaatgaata aggaaacacc tattccgata
     1921 aaagatatag acaatatgct taaaattctg aaggaatatg atttatacat cataggaaga
     1981 attgttgcat ttaaggacaa gaattttgcc gttaagagtc cggagcatgc catacagcta
     2041 aaatccggcg ggatatggct tgaccctaac agcggaaaaa ttccatggat aaatcccttt
     2101 gataaatatg tgtgggatta taatgtggca attgccaagg aagcatcact gctgggtttt
     2161 gatgaaattc aatttgacta cgttcgtttt ccggatggag ctaaaacata taatgctatt
     2221 gcggaatttc cgggaagaga cggcagagat aaagatgaag caatagctga ttttttagaa
     2281 tattctaaaa aagaattaga accttacaat gtaaatttag gtgcagacgt atttggtctc
     2341 gttacaagga catgggatga ttatcctgag gatataggcc agacttggat actgattggt
     2401 aaacatgttg ataatatttg tcccatggtt tacccgagtc attattcaac agggtggtat
     2461 aatttagaaa atcctaatgc tcatccttac ttggttgtaa aaggtgctat ggaagaagca
     2521 atagaaaaaa attcctccat ggaaacacct ccaattatac gaccttggat tcaggacttt
     2581 gactgggatg gaattgcata cggtcctgat aaagtaagag accagataat agcagcaaag
     2641 gagcttgggg tatatgaata tatgatatgg aatccaagca atgtttatga tccatatgca
     2701 ttcatggtta cagaaaagga aaagataacc gcttatccgt taaataaagg agaactggac
     2761 tacagagaaa aatcacctgc tgatgcagca gttaagtatt taaccggtat gcttcaagac
     2821 aggcacagct acacatattt aatgactcct gtttcagaca gagttaatga ttttgatgaa
     2881 tttgtaaaac ttcgggaaga agataatatt aagcttttaa gctacaaggt ttacggctat
     2941 gaaatggatg caaatgacag caataaggca aatgtaagct tatattataa aatcgaagtt
     3001 aaaaacggag atgcaacaga gataatagaa aaagacaatg ttgaatggca agcaataaga
     3061 gaacttaaca tctggaaagt aaaagtaaac tgggatattg gaaaaaatgt acactgaatt
     3121 aaaacacatg tacataaaat aaagtacaaa aaataaatca aaaagcttga caagctgaaa
     3181 tttattgtta taatattctt taaatattta aaagcaggta agcttgcaga aggtgaggac
     3241 gatatgaaaa aatacaatgt agcaatattg ggagcaacag gcgcagtagg gcaagaaatg
     3301 ctaaaagtgt tgggagaaag gaattttccc ataaatgatt taaaattgct ggcaagcaaa
     3361 agaagtgcag gagcaatagt tgaattcaac ggggaagaat atgcaatcga agaagcaaca
     3421 gagtcatcct ttgaaaatat agatgttgtg ttgtgtgccg ctgaaaatga tataagcgaa
     3481 aaattgtctc ctgctgctgt aaaagcaggt gcggttgtag ttgataattc aagtgcattc
     3541 agattatatg atgatgttcc cctggtggta cctgaagtta acggagaaga cgttaaaaac
     3601 catcagggaa taattgcaaa cccaaactgc tcaacaacaa tagctctaac ggcaataaat
     3661 gagcttaata agtattctcc aataaaaagg atgatagtta caacatttca ggcagtatcg
     3721 ggtgccggcg ttaacggtat aaaggaactt aatcagcaga ttaaggatat aagtgaagga
     3781 aaagatgtag aaataaatac attccagcat cagatagcat acaatgtaat tccccaaata
     3841 ggaagtttca atgaaatggg gtattcgcag gaagaaatga aattgctgaa tgaaggaaga
     3901 aaaattcttc ataatcctga cttaaaagtt aattgtacat gtgtaagggt tcccgtttac
     3961 agaagccatt ccgaatcaat tacaatagaa acagaaaagg aaataacggt agaaaaagca
     4021 agagagctgt tgtcaaaagc agcaggcgtg aagttaatcg atgatttgga aaacaataaa
     4081 tatccaatgc ctcttgattc atcagaccag gatttaattt atgtaggcag aatcagaaag
     4141 gatataagcg gcgaaaattc attggttctg tggtgctgcg gagaccaaat aagaaaaggt
     4201 gcagctacaa atgcagtgca aatagcagaa gttttggtta aagaaaattt aatttagttt
     4261 ttcccggtgc ctttttccct gtgccaggca ccattgtggt gcctggctcc aatgtatatt
     4321 tataggatat gaaagaatag catccatgct gagaataata ttcctgatag aacccatatt
     4381 gcaggcagca gttccttata ttttttattt tttattgcaa taattatatt tgatataacg
     4441 agtgctgcaa tgaaccatcc gagaaatttc atgtaaggag catatttaag aattgaaaag
     4501 gttacaattc ctttatacat aagcaaacat aagatgacaa acgaaacaat ttggaaaaag
     4561 gaattcaatg caaatgcaaa agaatattta ttgtttttat tactaaactt tttaatcagt
     4621 tctctaataa agatgataaa tgttacgatt ataaaaatta tcggtataat catttggata
     4681 attctatgct gccacaatgg aacttttaca tatgttatgg cagattctga agacataatt
     4741 attttatttt gcacttcctt aaattttagc aatcctaaat ctgacgaata caaatcctca
     4801 tcgattaaag tcatcttttg accattaata taatgcccgt cttttaccgt atctatatat
     4861 atcattttgt taggtataaa gtaacgcaga attttttcgg gagtattgaa gctggaccaa
     4921 ttatttacat ataaaccgcc tatcttaatt gatgagtcgg cattttttat agtgtattct
     4981 tgtttctcgc cataaatcaa gtcagtggct ttattcataa tattattaat ttcatgctca
     5041 ggtacataag tgttaaatga tagaaaaact cccgtatcag cttgtgaata aagggctatc
     5101 cttgtataaa aatttaaggt ttctcctttt ttatcataat aaactttatt gtcttgttct
     5161 ttcctgttcc agattagtcc tataccttca aattcctctg acattgtaaa ttgtttttta
     5221 aacagctctg ctttggaatc aggtgataat acacgattat catcaccgtc catcaaccaa
     5281 atcatatatt ttgccatatc ctctgccgtt gacacagccg agccctccgg atataagttc
     5341 atgtaaggct caatagtttc attgccattc ggcgaatatg cttttgaaac ataggcttta
     5401 tcatgcataa atctaaatgt tgtcctattc atatttaaag gctcaaaaat cttctcctcg
     5461 caaaataagg caaaatcctg ctctgtaata cattcaatta catatgcggc aagtgctata
     5521 ccgtaattcg aataagatat aacctcaccc ggcttatata tttgtcttgg caggtactta
     5581 cgtacactta tttcaagagg ttcagtatcg cttacattat aaaaagccat acctgtaacc
     5641 atatcttcaa atcctgcggt atgtgtgaga agctgtctca tagaaacagg ataagtaagt
     5701 tttaaatcta aatccagata aattgatata tcgctatcca tgttgagcaa ttctttttca
     5761 acaagaattt gagctgcaac cgcaacaaag gtttttgata ctgagccgat tcgaaatgca
     5821 attttttctc catcagaagc aatgccgagg ttatcatcag ccaagccata ccctttgcac
     5881 aattcgatat ttccatcctt tacaacagac agtacggcac ctttggattt tccttcatcc
     5941 actatccttt gaaccatctt atccaactga ttgtatgttt ctatatcggc tgccaaaacg
     6001 cacggttgtg cagctgagaa aaaaattatt ataactaata ctaaacttat tgcttttttc
     6061 ataatatcct cctttatttt tgaatttatt ttattttggc agcctgaaat ataaatgtta
     6121 tttaccttaa atttgcctta aattaacaag aaagtattta tcagatatta atattgtgct
     6181 atattgaaaa tataaaaaat tcttaaagag gggataaaag tgaatatatt agtggcagag
     6241 gatgaaatag atattagaaa ccttataaag ataaccttgg aggatgaggg ctattccgta
     6301 attaccgcat gcgacggtat tgaggcgtta aaaaaactga aggaaacacc tgtcgactta
     6361 gccatattag atattatgat gcctcggctt gatggattta acctgcttag aaaaataaga
     6421 gaaaacagca ccattcctgt tatatttctt actgcaagaa cagatgattt ggacaaggta
     6481 ttaggacttg aattaggagc tgatgattac attgccaagc ctttctccat gagtgagctc
     6541 attgcacgag tcagggctca acttaggaga aataatgaat acatgctgcc caaacaagaa
     6601 aataaaacct tgcttcaata tggtgattta acgatagata aagaaagttg ctgtgcttac
     6661 aaaaataatg agcaaataga actcaatgca aaggaataca aactgctctt acatttaatg
     6721 gaaaaccccg agagagtatt tacaaaaaaa cagctctaca atgcagtttg ggatgatgac
     6781 tattactatg atgataatac aatcatggtt catatcagtc atctgcgcaa taaaatagaa
     6841 actgattctc aaaatcccga atatataaag actatacgag gcttaggata taagcttcat
     6901 aaaacaggtg gatataatga aaaataaaat ttcccatcaa ttcctgttaa attatcttac
     6961 attttttctt atgtcattat tagcagcttt atttgcctat ttattgttgt cgtttgcaga
     7021 tgatgtgatt tcaaaaacac ttgtaaaaaa cagataccct tcagaactca tcatgaaaga
     7081 caattatgcc gatattgatg cagaatcagt agttgagcat ggcggtggag tacaggtaat
     7141 aaatgaaaac tatgaagtgg tgtattcaaa aggattagat actcttggaa agaagcagtt
     7201 ttctgtgagt gaatttactg attttcttgt aaaaagcaaa gagaaaggat ttaagtatca
     7261 ttatgatatt ctatataatc cgcaggcaaa gttttggctt attgtaacat tcccgacatc
     7321 aatgcgttta gatatttcct ttgtttacaa taaggaagct gcctcggagg atttaaaaaa
     7381 tgtatccttc gtattcattt ccgtgtttat tttatatttg cttctgcttg ggttgcttgc
     7441 ctttattttc tcaaaaatta catcgataca cataattaaa cccttaaaaa tgttaaagga
     7501 agggactaaa gcattaagag aaggtgatta ttctgcaaga gtttccatga atttaaaaaa
     7561 tgaatttgca gaattgcagg atacttttaa tgaaatggct gaaaaaatcg agaccgaaat
     7621 aaagctgaga gaacaatcgg aagctgaacg caagaaaata attatggata tttctcatga
     7681 tttaaaaaat cctctggcta ttgttgtcgg atatacggag ctatgtctta aaaaagcaga
     7741 gtcactagat aaagagctta ttaatcatct tcaaattatc tttaaaaaca gtaaaagagc
     7801 aggtgttttg ttaaatgatt tgtttgaatt ctctaagttg gaaagctctg aattctccat
     7861 gaaatttcat aaaaccgaca tatgtgaata tttaaggcaa gcatgcggcg atatactgcc
     7921 tatgatagaa caagccggct ttgaatacag ctttgatata cctgaaaagg cggtatatgt
     7981 gatgctggac gtcagccaga tgaaccgtgt actattcaat ttagctgaaa atgcaataag
     8041 atacaatcag tcgggaacca gtttatcagt aagtttatat gatgataaaa caaatgtact
     8101 aatttatttc agtgatgatg gtataggaat tccgtcacat cttgcaaaaa atattttcaa
     8161 accctttgtc aggattgatg actctcgtaa ttctcagacg ggaggaacgg gcttagggct
     8221 gtcaatagca tataaaataa ttaagtcaca tggcggagac atggaattaa aaactgattt
     8281 aaataaagga tgcacttata ttatcactct tccaaaaatt taatatacct ccaaaaaatc
     8341 ctttagagtt tttatgaagt aatctataag attgtaagca tacgagagta atttaagtgg
     8401 acaaatgttc gattttgtag tattattaat aagtaaaggg gaggtgtcca tgtacgattc
     8461 agttattata tcattgcttt ctttatcatt gataatcaat atttttttac ttataaaaca
     8521 aataaaaaac aatacaaacg agcgcataat atcagagctt aaaatttatt taaaggataa
     8581 taatttggat ttaattgatt cagtaaatcg aaaaatcgct gagtctgagc agaagcagct
     8641 gagggaattg ttgacaaaca agctggaaac agttgaaaga atagatagaa actccaataa
     8701 gctgacagaa tcattcgcaa aattcagcgc tgatacatct aaaactctaa atgaaaattt
     8761 tatatctttg aatgaaagag taagccaaaa tcttgacagg ataaacttaa gagttgaaga
     8821 aagattgaac gaagggtttg agaaaaccaa caaaacattt gtcaacatac ttgaaagatt
     8881 gtcaagaata gatgaagcac aaaagaaaat agacagcctt tccacaaata tagtttcctt
     8941 gcaggatgtt ttaaccgata aaaaaagcag aggcactttt ggagaagttc agcttaatca
     9001 tattttgtcg gttgtctttg gggaaaagaa cgataaaata tatgaaatcc aaaaaaaatt
     9061 agaaaattca atgattgccg atgctgtttt atacataccg gagccagtag gaatgctttg
     9121 catagactcg aaatttcctc ttgaaaatta ccagcgcatg attgatgtca atatttctga
     9181 agctgaaaga aaacaatatt caagagactt taaattaaat gtaaagatgc atattaatga
     9241 tatatcttca aaatacataa ttccgggaca aacttcagac caggcaataa tgtttattcc
     9301 ggcagaagca atatttgcgg aaataaacgc atatcatcag gacttgttgg attatgcagg
     9361 cgaaaagaaa gtttggataa cttcaccaac gacattaatg tcagttttgt caacggttca
     9421 ggttgtttta agaaatattg agagagaaaa gtatgcagga ataatacacg aagagcttaa
     9481 taaattagga aaagaattta agctctacaa ggacagatgg gagtctttat ctagaaatat
     9541 taaacgtgtt tcagatgatg tggataagat taacataact tccaacaaaa ttgaaaggaa
     9601 gttcgacaga atatcaaggg tagaaatggg ctgcgagcct gaaatcgcag aaatagaata
     9661 cagcgaaata tgaattgcaa aaaatacttg cattttactt ttgtttatgc tactatctta
     9721 aagtagcatt ttgccgcagt gatggaatgg cagacgtgac ggactcaaaa tccgttggtg
     9781 gcaacaccgt gggggttcaa gtcccttctg cggcataatc aatataaaat cgaccttata
     9841 gtcgatttat ttttttatgt gttaaaatgc gttagtaaag aaaattacac ccaagcgata
     9901 attatgaata ttgttatatt gcatttttaa ctatatattt atagtttttt gtgatataat
     9961 tttttaacta agaaataaag gtgaaaagat gagtaaatta tatgtggtac ctacgcctat
    10021 cgggaattta ggcgatatga caaacagggc agttgaaaca ctgaattctt gtgatttaat
    10081 attagctgaa gataccaggc atactcaaaa gctgctgaat catttcaata ttaaaacaaa
    10141 aatgatttca tatcataaat ttaatgaaaa gcaaagaagc gaagaaataa tacataagct
    10201 tttgaatgag gatatcaatg cagccttggt aacagatgca ggaacaccgt gtatttcaga
    10261 ccccggatat gaattagtga gggaagtcag ggaaaagggt attgaagtaa taggtttaac
    10321 cggtgcatct gctcttacaa cagccctttc catatctgga attgaaacat acgaatttgc
    10381 attttacgga tttttgcccc gtaaaaaaag cgaactggat actgcattaa aaagagtgct
    10441 taacaatccc gttaaaacat ttgtttttta tgaatctcca aagaggataa taagtttgat
    10501 tgaaaatata aaaattcata tgccgaattc aactctttgt gtttgcaagg aattgacaaa
    10561 gattcatgaa aaaacttttt atggaagtgt ggatgatgta cttgagcagt taaacaatca
    10621 tggtgatgtg gaaaaaggcg aatacgttat tgtaggatat aacaatgatt ataaagaaaa
    10681 gggtgataca gacatacagg taagcctcca tgcaatttta tttgacaaat tattcaaaaa
    10741 tgaatattct ttaaaagaag ccgtaaatga ggcagcatta gaaggaattg cttctaaaaa
    10801 tgaagcgtat aaggcttcgc tggaaataaa aaaaattatt gagaagttaa ggtagggcat
    10861 atgataggca caatcgcaaa tgcacttgca ataatagtag gcggaataat aggactttta
    10921 tttaaaaaca taatacctga aaaaatatcc gaagcacttt taaaggctat cggtctggca
    10981 gttattggca taggcataaa tttgatgcta tccggtgaaa attttacact tttaatcata
    11041 tcgatggtaa taggaacaat aataggtgaa ggcattgata ttgaaaaaaa actggataaa
    11101 atcggagcat ttatcgaaag taaaatgaaa aacaaggaaa gcaatgtagc gcttggattt
    11161 gttgcatgca ccctggttta ctgtgtgggc tccatggcta ttgtgggctc aattcaaagc
    11221 ggattgacgg gaaaccatga aattttattt tcaaaagcag tgcttgacgg cattacagcg
    11281 gttacttttg cggcgacaat gggtgtgggt gtagtttttt ccggcataag cgttcttgtg
    11341 tatcaaggta cgataacaat gcttgcaagc attatgcaat ctttgttaaa ccctgtggta
    11401 gtaagcgaga tgactgctat aggcggtgta ataattatgg gaatcggtct gaattttcta
    11461 atatcaaaca gaatgagggt aggtaatatg ctgccgtcta tatttatacc tataatatac
    11521 tatatgattt tgatgaggta ataaattgga tagaaattta gttttaaaac caagtgctat
    11581 aatgtctata tgtctaattt ttattatcgg ctcaattatt ttatcaatcc ccctatattt
    11641 tggttttttg gcggggattg ttcttacttc tacagttctt tataaaagcg gttaccatat
    11701 gcgggaaatt tacgaaacat ctgtgtctgc attaaaggat gttaaacgtt tattgattgt
    11761 aatattgttt ataggcgcaa ctacttcaat ttggcttgca tcaggtgttg tgcctacaat
    11821 tatgtactat ggttttacat atatggaggg agtaaacttt cttttggcgg tatttctaat
    11881 tacgagtgtt gtttcatatt ttttgggcac ggcaatcgga actataagca ccgttggaat
    11941 atccttaagc ggaatcggat taagtatagg aatacctcat aatttgatgg ttggcgtctt
    12001 ggtttcggga gcttttatag cagataagat atctcctttg tcgggtttgg taaatctcgg
    12061 aatgactacc gtcaacagaa actataaaga actgttcaag agtatgatta taacattgat
    12121 accggctata gcagcaacat cagtaatata ttttattata ggttcaagtt atattgctaa
    12181 aacagaaaat cttataatgt ataaacaggc aattcaagaa ggatttaata catcgccggc
    12241 attatttatt ttacctgcag taattctaat tttatccatt tcgggacttg attcattaaa
    12301 aacattttct atcggtattg gggtaggtgc aatacttagt attatatttc agcaggaatc
    12361 gttattatca attattaagg cgcttatatt cggttatcga ggcaacacac cttccgaaga
    12421 attaaacaga ctcttgctca gcggaggtat gatatcaatg atagaggcaa tattcatagt
    12481 tgccggagca tatttattga ttaaactttt tgaaaagggc aatgtgcttg tgcctttaat
    12541 ggataagctg gtttacggaa taaacaataa aatgagttta ataggcagaa caggactgat
    12601 aagtatattg atgacagtgg caacatgtga tcaaacatcg ggaattatat tacccggcac
    12661 catattgcag gaaaaataca aggagctgaa attggacaat gctgtcttgg caagaaccat
    12721 atttgacacg ggagttattg tggcacctct aatgccttgg aatataaatt catttttaat
    12781 aaaacccgta atgggaatta ctgcagccca atatgctcca tttgctgtat tatgtattgt
    12841 ttgtcccttg attacaatgc tggtttccta ttttcaatat aggaattcat aaccgaaatt
    12901 tgtttcatga ttctcttatt gtactacagt gttacaatta ttttgctttt ttaaaaaata
    12961 cagttttatt ttaagtaaag cataataaaa gttaagaatc ttcgaatttg tttttgatat
    13021 tgaatgtatt atacagtagc tatataatac acttgtgata tagtggttac aattaataaa
    13081 acatagtaac acaaataata tttaggagag tgccatgaaa aaattaacgg acaattcaaa
    13141 tgttttaaag tgggttgacg aaataatttc tatgactaat ccatcgaatg tagtttggat
    13201 tgacggcagt gaagaattat acgaagaact tcgcaaagaa ggatgctcaa caggcgaact
    13261 cgtaaaattg aacgaagaaa tttaccccgg atgctattac catcgctcgg caaaagatga
    13321 cgtagcaaga atggaagaca aaacatttat ttgttccaga aaaaaggaag atgcaggtaa
    13381 tacaaacaat tggaaaagcc ctgaagaggc ttataaattg ggaagagaaa ttttcactga
    13441 ttccatgaag ggaagaacaa tgtatattat tccctattca atggggcctg ttggttcgcc
    13501 cttttcaaag ataggaattg aaatcaccga ttctatttat gtcgttttaa gcatgaatat
    13561 aatgacaaga gttggaaaag aagtacttga tgcattagga gattccaatg atttcgtgag
    13621 aggcgtacac ggaaagtgca cattggatat caacaaaaga tatattttcc attttcccga
    13681 agacaataca atatggtcat gcaattccgg ctatgggggc aatgttctcc tcggcaaaaa
    13741 atgctttgca cttcgcattg cgtcatacca gggccgaatg gaaggttgga tggctgagca
    13801 catgctcatt gtaggaatag aaaatcctca gggagaagta aaatacatag caggagcatt
    13861 cccgtcagca tgcggcaaaa cgaatttggc tatgcttatt cctcctgaaa tttacttaaa
    13921 caaaggatat aaagtttgga ctgtaggcga tgacatagca tggcttcgaa ttaaagaaga
    13981 cggaagacta tgggctataa atcctgaagc aggctttttc ggagttgcac cgggaacaaa
    14041 tgcaaaatca aattataacg ctcttatgtc tacaaagaaa aattcgattt ttacaaatgt
    14101 tgtattgaat ttggacgata acacggtatg gtgggaaaaa cttgacaaca atcctcctga
    14161 aaatgctctg gactggatgg gaaatccatg gaacgggaaa acatcaaagg aaaacggagc
    14221 acatcctaac tcaagattta cggcaccggc taaaaattgt ccgtgcattt cacctgaatg
    14281 ggaatcaccg gagggagttc ctttatcggc aataatattc ggaggaaggc gtgcaaagac
    14341 tgctcccctt gtttatcagt caagggactg gcagcatggt acttttgtag gctcgatact
    14401 tgcatcggaa actactgcag ctgcatcagg aaaagtcgga gttgtaagaa gggaccccat
    14461 ggccatgctt ccattctgcg gatacaatat gggagattat tttaaacact ggcttgaaat
    14521 gggtaaaaaa atgagcaatc cgccaaaaat tttccatgta aactggttta gaacaaatga
    14581 agaagggcaa tttatttggc cagggtttgg agataatttc agggtaatag aatggatgct
    14641 caaaagatgt gaaaataagg cggatgcaaa tgaggcacct atcggttatc tgccaaaacc
    14701 cgaagacatt aatattgaag gtcttaattt atcagcagac acattgaaat cacttctttc
    14761 aatagacacc gaatcatggc ttgcagatat aaaaaatata gaagagttct atgctaaaat
    14821 aggtgatgcg atacctcaag aattaattga agaacttaga actttagctg ataatttaat
    14881 cagaagtgca gcattggttt aatgttatac agagcaatca acaatataat aactaaaaat
    14941 atttagcgaa catttgcagg acacgactat aattcttagc gaatgaaggt taaaaatccg
    15001 ttaagcagct tgcattgttt gagcgcagcg agtttgcaag ctgtaggatt tttagccgga
    15061 atgagcttta gaattattcg tggccgaaac cgtgagcgta tatttttagt tatgtttctt
    15121 taaataatcg gacaatctca aataggaata caacagttta cgagggcgta gccctcgtta
    15181 tttttatgat tggtaaaata ttactgaaaa ataattgttg acaaagggat tattataagt
    15241 tattattgct attaatattt aaagcgttga agcagagaaa agtatttcag ac
//
LOCUS       DAOSHP010000038        14951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3285_length_14951_cov_9.389702, whole genome shotgun sequence.
ACCESSION   DAOSHP010000038 DAOSHP010000000
VERSION     DAOSHP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14951
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3285_length_14951_cov_9.389702"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(9..254)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(360..1739)
                     /locus_tag="PLB33_05245"
     CDS             complement(360..1739)
                     /locus_tag="PLB33_05245"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOG62962.1"
                     /translation="MNENLLSGIYKNNEEMNVNFDEQGKKIVVLDDDPTGTQTVYDIP
                     IYTRVDYEIIQKIFQDNNIKAFYILTNTRSMSKEKVMELDSKIARNIDLVSKRTGIDY
                     ELIVRGDSTLRGHFPLELNIIKDNISKKIDGYFLIPFFQEGGRITVDDVHYVKENNTF
                     IPVGETQFARDHTFGFTSSNLKDYVEEKTNCLVLKDQVVSISLEELRSNSSAVFNKIM
                     DLNGRICIVNAENYSDIQAFVNGLIKAEYQGKNFLFRTAASFVKSRIGLKDNGLLDGN
                     TLNIIDNRGGLIVAGSYVNKTSQQINHLMKETSIYSIEIDVLSILTESDNSNIIKKIS
                     QKIDENIIRGIDTLIYTSRSVILGNDNTPNLDIGNKISLYLAKIVKQLKEAPAFIVAK
                     GGITSSDIATEGLNIEEAIVCGQIANGVPVWKSGRESKFYGLVYVVFPGNVGDEDELT
                     NVIRRLHKK"
     gene            complement(1807..2631)
                     /locus_tag="PLB33_05250"
     CDS             complement(1807..2631)
                     /locus_tag="PLB33_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOG62963.1"
                     /translation="MRKFKVALIQTMVYDDKEKNVKNAVKMIGKVSKEGADIAVLPEM
                     FCCPYNTSYFRKYGEEEGGYAYKELSKAANSSGIYVVAGTIPELERTHVYNTSYVFDR
                     KGKRVAKHRKMHLFDIDVEGGQYFKESDVLSPGRDVTVFDTEFCKIGLGICYDIRFPE
                     LSRLMVLEGAEVIIFPAAFNMTTGPAHWELNFRGRALDNQVYTVGVAPARDMESSYHS
                     YGNSIVVSPWGNVINRMDEKEGYIIQEIDLEYVKKVRNELPLLKHLRRDLYTLSKN"
     gene            complement(2701..3330)
                     /locus_tag="PLB33_05255"
     CDS             complement(2701..3330)
                     /locus_tag="PLB33_05255"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG62964.1"
                     /translation="MTKIYLTRHGETVWNRQCRFQGHKNSDLTEKGILAAELLADRIE
                     EIELDYIVSSPLMRAYNTAEIIRGNKNIQIIKHDGLKEINLGKFEGMSYKDIQKNYSK
                     LLAEIEKDPFNNRYPNGENLQEFYKRVVKAFNEVIDKYRNKSTLIVAHGGTIKCIEAY
                     IRKFKLSSDWMGNVVKNCSLSYLEIDDNNEIKEIFYNDTKHLEGSAALN"
     gene            complement(3353..5320)
                     /gene="ligA"
                     /locus_tag="PLB33_05260"
     CDS             complement(3353..5320)
                     /gene="ligA"
                     /locus_tag="PLB33_05260"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOG62965.1"
                     /translation="MDKKLLLMKEKIRLLNEANKAYYQEDREIMSNYEYDKLYDELKK
                     MEEETGIVLSNSPTIRVGYELLSNLPEERHEKVMLSLDKTKEVTALKDWLGEKTGALS
                     WKLDGLTIVMTYEGGRLVKAVTRGNGEVGEVVTNNAKVFTNIPLSIAYKGSLVLRGEA
                     VISYSDFYKINETIPEAAGKYKNPRNLCSGSVRQLNNRITAERNVGFYAFSVVKADEV
                     DFKNSRIEQLLWLNSQGFSIVEFKKVTQENIEETVNWFEENIRENDLPSDGLVLTYDD
                     IKYGESLGSTAKFPKDSIAFKWSDEIKETTLLEIEWSASRTGLINPIAVFEPVELEGT
                     TVSRASVHNLSIMEDLELGIGDRINVYKANMIIPQISDNLTRSNNIEIPEHCPVCGGE
                     TSIKNDNDIKTLYCINDECLAKQIKSFTHFVSRDALNIKGLSEATIEKLIAKGLIKEL
                     ADIFHIERYKDIITEMEGFGEKSFNNLVNSVNKARKTTAARLLYSFGITNIGLSNAKL
                     ISRKFGDDWEKIQNAKYEELIEISGIGEVMAKNYVKFFSDEKKKVIVEDVLKEVEIET
                     SSKTSSTQIFENYNFAITGAVEHFKNRDELKAAIEERGGKVTSSVTSKTNYLINNDNL
                     SNSSKNKKAKELGIEIITEEQFLKWMNTGVR"
     gene            complement(5335..7542)
                     /locus_tag="PLB33_05265"
     CDS             complement(5335..7542)
                     /locus_tag="PLB33_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOG62966.1"
                     /translation="MEILNSLNDKQREAASHDKGPILVIAGAGTGKTRVLTHRTAYLI
                     ESGKAMPWEVLAITFTNKAASEMKERIGRLIDYSIDRMWIGTFHSVCVRILRSFIDRI
                     GYDKNFIIYDDDDQKIVIKDCIKELELDPKLYNYNSIKALISNEKNRRNTPDNYIKDN
                     YLNYANRNKGEVYALYEKKLKAANALDFDDLILKTLELFEKNEDILKHYQERFKYILV
                     DEYQDTNNAQYNLIRILGKKKNGESNVFVVGDEDQSIYGWRGADISNILNFENDFKGA
                     KIVKLEKNYRSTDVILNAANGVIKNNYQRKGKNLYTEQKKGNLIRIIETDNERDEAFT
                     TAALIRKEHRENNINYSEIAILYRTNAQSRALEEGLIREGIPYKIVGGVKFYDRKEIK
                     DIMAYLRLILNQKDDVSFERIINVPRRKLGQKSTDTISNYAEANGVSKFEACFDFDKM
                     GLIPSAAKSIESFVSMMEMLMIKKDAMPLSEFIISVIEDTGLKDMLLQDETVEGRSRV
                     ENIDEFLSAAKDFEERYEENTLEDFLAHISLLADIDKTDSIKDVVTLMTVHSAKGLEF
                     DTVFITGLEDGMFPSIHFDGEDNVEEERRLFYVAITRAKRMLYITHARDRIRFGSHEM
                     RMKSRFLEEIPEECIEESDPYSYANKKLEQLESQIARKTSFFEYRAEITEKEKPKIKS
                     DKFEPGDKIMHKSWGKGTVVQIKDSGDDKIIVAAFENKGIRNLSLKLAPIEKI"
     gene            7711..8151
                     /locus_tag="PLB33_05270"
     CDS             7711..8151
                     /locus_tag="PLB33_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG62967.1"
                     /translation="MKKILVPVDGSAASQKAVAQAFDIGRKYNSDVVVFHVFIEHDID
                     MYNKFGVVIDPDMNKLKKRLKENESKMLDSIINQYDNTGINLKKKIVSGVAYEEILKE
                     AKDGNYDLIVIGQRGYSKIKRFFVGSQTVRVISDATCPVLVVHE"
     gene            8168..8620
                     /locus_tag="PLB33_05275"
     CDS             8168..8620
                     /locus_tag="PLB33_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG62968.1"
                     /translation="MKKILIPIDGSPLSVKIGEKAVELGKLIDAELIFITVINMPSED
                     KYAYFGMTVENQFSANRKEMLNKLMQQESKMLNIIVRNLDTGNLKVTQKLLVGRPAAE
                     ILKFAEEENINYIFMGRRGFSPVQRFFVGSVTQKVISAAPCPVIVING"
     gene            complement(8664..9653)
                     /locus_tag="PLB33_05280"
     CDS             complement(8664..9653)
                     /locus_tag="PLB33_05280"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOG62969.1"
                     /translation="MKYIENNSTDPHYNLAFEEYVFKNLDNNEDFVLLWRNGPSIIVG
                     KNQNTIEEINMEFVKNNNINVVRRVTGGGAVYHDLGNLNFSFITKAQSNLIDFKTYNI
                     PIVKSLEKLGVDCELSGRNDIVIDGKKFSGIAQSIIKGRVLNHGTLLFDSKLDILSKA
                     LNVKRDKIESKGVKSVSSRVTNIKPYVKDDIDVLQFKQILLKNIFEYFNQPVEIFELS
                     DEDRNKIQKMVDERYGTWEWNFGRSPEFNYKGYDRFEGGGVEVRLHVCNGIIESCKIY
                     GDFFGKGDVKNLEDKLLGVKYDINEINKAIEDISIDEYLGRITKEEFLRCLFS"
     gene            complement(9805..10521)
                     /locus_tag="PLB33_05285"
     CDS             complement(9805..10521)
                     /locus_tag="PLB33_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOG62970.1"
                     /translation="MNLKNYNIQNNNTAIDRDKTKIVFMFDDGWRSVYSEAYSLMKEY
                     SYKGSVSIIPSLIVEDEYMSYKELAELYLEGWDLLNHSYSHRADAYNNTDELLSDFDK
                     SRRWMDNRYFGKCSDMVVMPHGEINPYLIRQLKNAGYRSVRTSDNIIVLDSFNYYYYP
                     ITSINPVTNISANEVTAQLKQGINELKTFVLIFHKIGYEDDEFGMTYNKDMLKQIIMF
                     IDRHSDKFQVLTYSQLDFNK"
     gene            complement(10659..11663)
                     /locus_tag="PLB33_05290"
     CDS             complement(10659..11663)
                     /locus_tag="PLB33_05290"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOG62971.1"
                     /translation="MYDKNYISNYYHMNTEDIVIVNNPTGIAGVEIQEGYAGDEMHYN
                     HMIKYIEEHGAKSQKDYEYIKTKMDVDNFIEYNILQIYCDNRDWPGNNVRIWRKRTQA
                     YEPNSLYGHDGRWRWLVFDLDYGFGLFKGESPVQNNSLEMATQADGPYYPNPPWSTFL
                     LRSLLENESFKNQFINTFADRLNTIYKPDVVINRIEAMEEIYYPNIANHIARWKLHNN
                     KVENWSNEIEVMKKFARYRPQHIYQHIINYFGLTGTASIKVDMNEGGAIKINSVNILD
                     KDTLWEGTYFTDIPIKVQAIPSPGFVFAGWEGVVQSEEDTVVINLKQNSYLKAHFKEI
                     "
     gene            complement(11699..12820)
                     /locus_tag="PLB33_05295"
     CDS             complement(11699..12820)
                     /locus_tag="PLB33_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOG62972.1"
                     /translation="MFFYAVLFLSTFFSIYHLISSLRFKDRRTLYQVPQKGMSILVPC
                     YNEALIIRYTIQGLLNIDYDNFEVIFINDGSLDNTFEILNEILDLQVLEAEPISLWTD
                     QMKGIYKSNKYSFIYVIDKYNGGKASSLNTGILFSKYELIVTMDGDCILEKNALDSMN
                     KIFEDEDIIASGGVVQIMQMFKLDERQKPIVLIQALDYIKGFYIYKASLAYNDALSII
                     SGAFGVFRRKVLMEVGGYRSGLGEDIDITIRFQEYAKAHNKKLVFNKSATCYTECPES
                     LKGLINQRVRWQKGFIDAIINNHNFLFRNVFNNSLCFLMIIDALLSNSFATIVFVINC
                     ILIFMKIIYGYPLYMFAYFLTTIAYNFIASIIAIKGYYI"
     gene            complement(12821..13642)
                     /locus_tag="PLB33_05300"
     CDS             complement(12821..13642)
                     /locus_tag="PLB33_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOG62973.1"
                     /translation="MIKRYIKILLTTLFLVTILLVIIKEFKKSVHEIRGEYTKKQSVN
                     SSINGQKPVYNTPDIELNEIDENKHSWIAIDRKDSYNNELQLYSRDNVTFDEDSIVII
                     SKKEIIDDKIYTSGLVESTEAFKYGYFEFEIEISKGKGLFPAIWLLPISGGPLPEIDI
                     FEMIGSEPNKFYGVVHFEENNMRGRDYFEHMVSEKEQYTVALKWDAEFLTWYIDEQQI
                     YTTTKGVPHEYMYIIINQAVGGNWPGSPDDNTIFPNHFKILSTNINPTFREGRDL"
     gene            14030..14911
                     /locus_tag="PLB33_05305"
     CDS             14030..14911
                     /locus_tag="PLB33_05305"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOG62974.1"
                     /translation="MINKTISLILALMLMATPHVKCPEVIKILSIGNSFSQDCVYYLY
                     DIAQSAGVNVIIGNLYHSGSSLERHDKNAKENLKAYSYQKWTSSEMVEEKNKTMKEVL
                     LDEDWDYITFQQSSENSGLYDTYQPYLNNLINYVKSISKNPDVKFALNMTWAYSTRSS
                     NKGFAYYNYSQFNMYRCITESYKRAIEETEIDIIIPCGTAIQNARTNEHLNSIGNQLT
                     NDGYHLENGIGKYIAGLTFFETLVINDKNLDLNIYDDVTFIPETKNERADLADIAKKS
                     VIKAVNYPYIITKIRAD"
BASE COUNT         4517 a         2825 c         2057 g         5552 t
ORIGIN      
        1 tagatgtata tagggacgag aggtctttcg cggtaccacc ctaattcacg ataaaaaaat
       61 atcgcctcat tcaagtcgaa aaaacaaaaa agttttttga actctattct tgtaacgcag
      121 aaatacgtcc tcgtctactt aattcgactc ggaagctcag gagtgatcat tatatctcat
      181 aacaccgatt cccaccaacc atcggctctc tgaagttatt ttccggatat ttttgtctcc
      241 gtcaaagctt taatcaattt tttcatatta tatacacagg atattgtttt gtcaaccatt
      301 ttttaaaaag aaaaataccg gtctttcatc gtacttattt atcatgtagg gggaaatttt
      361 tattttttat gaagtctcct aatgacattt gttaattcat cttcatctcc aacattgcct
      421 ggaaagacta cataaaccaa tccgtaaaat ttactctccc tgccagattt ccacacgggt
      481 acgccattag ctatttgtcc gcaaactatc gcttcctcaa tattcaaccc ttcagtcgca
      541 atatcacttg atgtgattcc gccctttgca acaataaaag ccggggcttc ttttaactgt
      601 tttacaatct ttgcaagata aagagatatt ttattaccaa tatctaaatt aggggtgtta
      661 tcatttccaa gtattacaga acgtgaagtg taaatcaagg tatcaattcc tctaataata
      721 ttttcatcaa ttttttgaga aattttttta attatattac tgttatctga ttctgtaaga
      781 atagacagaa catcaatttc tatgctgtaa atagaagtct ctttcatcag atgattaatc
      841 tgttgactag ttttgtttac ataggacccg gcaactatca aacctcctct gttgtcaatt
      901 atgtttaacg tattcccatc taacaaacca ttatccttta aaccaatcct tgacttaaca
      961 aaagaagcag ctgttctaaa gaggaaattt ttcccttgat attcggcttt tatgagtccg
     1021 tttacaaatg cttgaatatc actatagttt tctgcattaa caatacatat tcttccgttc
     1081 aaatccatta ttttattaaa aactgcactg ctgttgcttc ttaattcttc taaacttatt
     1141 gatacaactt ggtcttttaa tactagacaa tttgtttttt cttctacata atcttttaag
     1201 tttgatgaag taaaaccaaa tgtatgatct cttgcaaact gtgtttctcc tacaggaata
     1261 aatgtattat tttctttaac ataatggaca tcatcaacag taattctccc gccttcttga
     1321 aaaaatggta tcaaaaaata tccatctatt ttttttgata tattatcctt tattatattt
     1381 aattctagtg ggaaatgtcc tctgagagtt gagtctcctc ttacaattaa ttcataatct
     1441 atacctgttc tcttagatac caaatcaata ttcctggcaa ttttggagtc gagttccatt
     1501 actttttctt ttgacattga tctggtatta gttaaaatat agaatgcttt aatattatta
     1561 tcctgaaata ttttttgaat tatttcataa tctactcttg tatatatagg tatatcataa
     1621 accgtttggg tacctgtcgg atcatcatct aaaactacta tctttttacc ttgctcatca
     1681 aagtttacat tcatttcttc gttattttta taaattcctg acaataaatt ttcattcata
     1741 acaatctcca attccacaga taaatagtct gtagtaaatt gcccaaacca ttaatttaca
     1801 atataattag tttttgctta atgtatataa gtctcttctt aaatgcttta atagaggcag
     1861 ttcatttctt accttcttta catattcaag gtcaatttcc tgtattatat acccttcttt
     1921 ttcatccatt ctgtttatca cattgcccca tggtgagact actatggaat taccatagga
     1981 gtgataggat gattccatat ctctggcagg agcaactcct actgtgtaca cctggttatc
     2041 aagggctctt cctctaaaat ttaactccca atgagcagga ccagtggtca tgttaaatgc
     2101 ggcaggaaaa ataataacct cagctccttc caataccatg agcctgctta attcaggaaa
     2161 tcttatatca taacatatac ctaaaccgat tttgcaaaat tccgtatcaa agactgttac
     2221 gtctcttccg ggagagagaa catccgattc tttgaaatat tgtcctcctt caacatcaat
     2281 atcgaataaa tgcattttcc tgtgcttggc aactcttttg ccttttctgt caaacacata
     2341 tgatgtattg taaacatgag ttctttcaag ctcgggtatt gttccggcaa cgacataaat
     2401 acctgaactg tttgctgctt tacttaattc tttataagca tacccgcctt cttcttcacc
     2461 atattttcta aaatatgaag tgttataagg gcagcagaac atctccggta acactgcaat
     2521 atcggcgcct tcctttgaca ctttaccaat cattttcaca gcatttttaa catttttttc
     2581 tttatcgtca taaaccatgg tttgaattaa tgcaactttg aattttctca tagcaccctc
     2641 ctcttataaa tacttgccaa ttttctacct tattctgttt ttcccgggaa atttcgacaa
     2701 ttaattaagt gctgcgcttc cttccaaatg ttttgtatca ttatagaata tttcttttat
     2761 ttcattatta tcatctattt ctaaataaga aaggctgcaa ttcttaacta catttcccat
     2821 ccagtcactg cttaacttaa attttcttat ataagcttcg atacatttaa tggtacctcc
     2881 atgggcaact attaaggttg acttgtttct gtatttatct attacttcat taaatgcctt
     2941 cacaaccctt ttgtaaaatt cctgaaggtt ttctccgttt ggataacggt tgttgaaagg
     3001 gtccttttca atttcggcaa gcagcttaga ataatttttt tggatatcct tatagctcat
     3061 tccttcgaat ttgcctaaat ttatttcttt taaaccatca tgttttataa tttgtatatt
     3121 cttattcccg cgaattattt cagcagtatt gtaagctctc atcaaagggc ttgaaactat
     3181 ataatccagc tctatctctt caattctgtc tgccaataat tctgctgcca atattccctt
     3241 ttccgttaaa tccgaatttt tatgtccttg aaacctgcat tgtctgttcc agactgtttc
     3301 gccatgtctc gttaaatata tttttgtcat atcaattccc tttctatctc gtctatctta
     3361 cacctgtatt catccactta agaaattgct cttccgttat aatttctatt cctaattctt
     3421 tagctttttt attcttggaa gaatttgata aattatcgtt attgattaaa tagtttgttt
     3481 ttgaagtcac agaagaggta acctttcctc ctctttcttc aattgcagct tttaattcat
     3541 ccctgttttt gaaatgctca acagcccctg taattgcaaa attataattt tcaaaaattt
     3601 gtgttgatga tgtcttagag cttgtttcta tctccacttc tttaagcaca tcttctacaa
     3661 taactttttt cttttcatca ctgaaaaatt ttacgtaatt tttagccatc acttctccga
     3721 tgccgcttat ttcaatcagt tcttcatatt ttgcattttg aattttctcc cagtcatcgc
     3781 cgaacttcct gctgattaat ttggcatttg acaaacctat attggtaatt ccaaagctgt
     3841 acaaaagcct cgctgctgtt gtttttcgcg ccttattcac tgaattgacc agattattga
     3901 aagatttctc gccaaatcct tccatctcgg taattatatc tttatatctc tcaatatgaa
     3961 aaatatcagc taattcttta atcaatccct ttgcaattaa tttttcaata gtggcttcgg
     4021 aaagtccttt aatatttaat gcatctctgc ttacaaaatg tgtgaatgat tttatttgct
     4081 ttgctaaaca ttcgtcattt atgcagtaca atgtcttaat gtcattatca tttttaattg
     4141 atgtttcacc gccgcataca gggcaatgtt ctggaatctc aatattattg ctcctggtta
     4201 aattatccga aatttgagga attatcatat ttgccttata cacattgatt ctatcgccga
     4261 ttccaagctc caggtcttcc ataatgctta gattatgaac actggcacgg cttacggttg
     4321 ttccttcaag ctccacgggc tcaaatactg cgattggatt tattaatccc gtccttgagg
     4381 cactccattc aatttccaaa agagtagttt ccttaatttc gtcactccat ttaaaggcaa
     4441 ttgaatcttt cggaaatttt gcggtacttc ctaatgattc tccgtatttt atatcatcat
     4501 atgttagaac aagtccgtcc gaaggcaggt cgttttctct aatgttctcc tcaaaccaat
     4561 ttactgtttc ttcaatattt tcttgagtta ctttcttaaa ttcaactatg ctgaatcctt
     4621 gactgttgag ccataatagc tgctcgattc ttgaattttt aaaatcgact tcatccgctt
     4681 taactaccga aaatgcataa aaccccacat ttctttctgc ggttattcta ttgtttagct
     4741 gccttacgga gccgctgcaa agatttctgg gatttttata tttacccgca gcttccggaa
     4801 ttgtctcatt tattttataa aagtctgaat aactgataac tgcttctcct cgtaaaacca
     4861 aacttccttt ataggcaata ctaagaggaa tattagtaaa tacctttgca ttgttggtaa
     4921 ctacttctcc aacctctcca ttacccctgg ttacagcctt tacaagcctt cctccttcat
     4981 aagtcatcac aattgtaagg ccatccagct tccacgaaag ggctcctgtt ttttctccga
     5041 gccaatcctt taaagcagta acttcctttg ttttatccaa tgaaagcata accttctcat
     5101 gcctttcctc aggcaaattt gataagagct catatccaac acggatggtg gggctgttag
     5161 acagtactat ccctgtttct tcttccattt ttttaagctc atcgtaaagc ttgtcatatt
     5221 cataatttga cattatttct ctgtcttctt gataatatgc tttgtttgct tcatttaaaa
     5281 gccttatttt ttctttcatt aaaaggagtt tcttatccat actttacctt ccgtttaaat
     5341 tttttcgatt ggagcaagtt tgagtgaaag attccttatc cccttatttt caaaagctgc
     5401 cacgattatt ttatcgtctc cgctgtcctt tatttgtact actgttccct tcccccaaga
     5461 cttatgcatt attttatcac cgggctcaaa cttatctgat ttaatctttg gtttttcttt
     5521 ttccgtgatt tcagctctat attcaaaaaa actggttttt cttgcgattt gggactccaa
     5581 ttgctcaagt tttttattgg cataagaata agggtcggat tcttctatgc attcttcggg
     5641 aatctcttcc aaaaaccttg atttcatcct catttcatgg cttccaaaac gaattctgtc
     5701 ccttgcatga gtaatataga gcattctttt ggctcttgtt atagcaacat agaagagtct
     5761 tctctcttct tcaacattgt cttcaccatc gaaatgaatg ctcggaaaca tgccgtcttc
     5821 aagacctgtt atgaaaaccg tgtcaaattc aagaccctta gcactgtgta cagtcatcaa
     5881 tgttacaacg tcttttatag aatcagtctt atctatatcg gcaagcaatg aaatatgagc
     5941 caaaaaatct tctaatgtat tttcctcata tctttcttca aaatcctttg ctgcggacaa
     6001 gaattcatct atattttcaa cacgacttct tccttcaact gtttcgtctt gaagtagcat
     6061 atctttcagc cctgtatctt cgattacgct tattatgaat tcgcttaaag gcattgcatc
     6121 ctttttaatc atcagcattt ccatcatgga cacaaagctt tcaatactct ttgctgctga
     6181 aggtataagc cccatcttat caaagtcaaa acatgcttca aatttcgaaa ctccatttgc
     6241 ttcagcataa tttgaaatgg tatcggttga tttttgtcct aattttcttc tcggtacatt
     6301 gattatcctt tcaaaactca cgtcatcctt ttgatttagg ataagcctta gataagccat
     6361 tatatcctta atttcttttc tatcgtaaaa ttttactcct cctacaattt tgtagggtat
     6421 cccttctcta attaaacctt cttccaaggc tcttgactgt gcattcgtcc tgtataaaat
     6481 tgcaatttca gagtaattga tattattttc cctatgctct ttgcgaatta atgctgccgt
     6541 ggtaaaagcc tcatctcttt cattgtctgt ttctattatt cgtattaagt ttcccttttt
     6601 ttgctctgta tacaggtttt taccctttcg ttgataattg tttttaataa ctccgtttgc
     6661 agcatttaaa attacatccg tagagcggta atttttttca agcttgacta ttttggctcc
     6721 tttgaagtca ttttcaaaat taagaatatt gcttatatcg gcacccctcc agccgtaaat
     6781 ggactggtcc tcatctccta ccacaaatac attgctttca ccgtttttct ttttacctaa
     6841 tattcttatg agattatact gagcattatt ggtatcctgg tattcatcca caagtatata
     6901 cttaaacctt tcttggtagt gctttaaaat atcttcattt ttttcaaaca gttcaagtgt
     6961 ttttagaatt aaatcatcga aatccaatgc attggcagct ttaagctttt tttcgtaaag
     7021 agcatatact tctcccttat tcctgtttgc ataatttaag taattatctt taatatagtt
     7081 gtcgggagta ttgcgcctgt ttttttcatt gcttataagt gccttaatac tgttataatt
     7141 atataacttg gggtctaatt ccaattcctt aatgcagtcc tttattacta ttttttggtc
     7201 atcatcatcg taaataataa agtttttatc ataacctatt ctgtcaataa aacttcttaa
     7261 tatgcgtaca caaacagagt gaaaagttcc aatccacatt ctgtctatgg aataatcaat
     7321 aagacgacct attctttcct tcatttcact tgctgcttta ttggtaaatg ttattgcaag
     7381 aacttcccaa ggcattgctt ttccggactc aatcaaatat gccgtccggt gagtcaggac
     7441 tcttgtcttc ccggtgcctg cccctgcaat aaccaatatg ggacctttgt catgagaagc
     7501 tgcttctctt tgtttgtcat ttaatgaatt tagtatttcc atttttctcc tatctgaaaa
     7561 agtacgggga catttttttt aattatatca cagtcaacca ttactctcaa gattctgatt
     7621 attgttttag gaatatattt gtttatggta aataaattta ggtataagta tattagttaa
     7681 tattctaatt attggctaag gagggacttt atgaaaaaaa tattggtacc cgtggatggc
     7741 tcagccgctt ctcagaaagc tgttgcccaa gcttttgata ttggaagaaa atataacagt
     7801 gatgttgtgg tttttcatgt tttcattgaa catgacattg atatgtacaa taagtttggt
     7861 gttgtaatag atcctgatat gaacaaacta aaaaagagat tgaaagaaaa tgaaagtaaa
     7921 atgctggatt caataattaa tcagtatgac aacacaggta ttaacctaaa gaaaaaaatc
     7981 gtttccggtg tggcttatga agaaatttta aaagaagcaa aagatggtaa ttatgatttg
     8041 atagttatcg gtcaaagagg atattcaaag attaaacgct tctttgtcgg ctctcaaaca
     8101 gttcgtgtca tatctgatgc aacttgccct gtcttagtgg ttcatgaata aataattgga
     8161 ggttattatg aaaaaaattt taatacctat cgacggctcg cccttatctg taaaaattgg
     8221 tgaaaaagcc gtagaattag gcaaattaat tgatgccgaa ttaattttca taactgttat
     8281 taacatgccc tcagaagaca aatatgcata cttcggtatg accgttgaga atcaattttc
     8341 agcaaatcgc aaggaaatgc ttaataaatt aatgcagcag gaatctaaaa tgctcaatat
     8401 tattgtaaga aatttagata ccggtaattt aaaggtaact caaaaattat tggtaggcag
     8461 gcctgcggct gaaattttaa aatttgcaga agaagaaaat attaattata tttttatggg
     8521 aagaagaggg ttctctccgg ttcaaagatt ttttgtaggc tctgtaactc aaaaagttat
     8581 ttcagcggca ccatgtccgg taatagtaat aaacggataa ttaggaaggg ttgggggaca
     8641 taccttgtcc ccgtacttta aaattagctg aatagacatc gcaggaattc ttcctttgtg
     8701 atcctgccaa gatattcatc aattgaaata tcttctatag ctttatttat ttcattgata
     8761 tcatacttaa cacctaatag cttatcttct aaattcttaa catctccctt gccgaaaaaa
     8821 tcaccataga ttttacagct ttcaataatt ccattgcaca catgaagtct aacttctact
     8881 cctcctcctt caaatctgtc gtaaccttta tagttgaatt ccggggacct tccaaagttc
     8941 cattcccaag ttccgtatct ttcatcaacc attttttgta tcttatttct gtcttcatcg
     9001 gataattcaa atatttcaac aggttgattg aagtattcaa agatattctt tagaagaatc
     9061 tgtttgaatt gcagcacatc aatatcatcc ttgacatatg gtttaatatt tgtcactctg
     9121 ctggatacgg actttactcc cttagattct atcttatctc tttttacatt taatgcttta
     9181 gacaatatgt ccagttttga atcaaaaagc agggttccgt gattcaatac tcttcctttt
     9241 ataatgcttt gtgctatgcc tgaaaatttc tttccgtcaa taacaatatc atttcttccc
     9301 gataattcac agtcaactcc caacttttcc aatgacttga ctatgggaat attgtatgtt
     9361 ttaaagtcta ttaaattgct ttgtgcctta gttataaaag aaaaatttaa attccccaaa
     9421 tcatgataaa cagcaccgcc gccggttact cttctgacaa catttatatt gttgttctta
     9481 acaaattcca tattaatttc ttctatagta ttttgatttt tacctacaat tatggaaggt
     9541 ccgtttctcc ataacaaaac gaaatcttca ttgttatcta aatttttaaa aacatactct
     9601 tcaaatgcta aattataatg tgggtctgtc gaattatttt cgatatactt catgctgcac
     9661 tccttgttaa cataattgaa attatgtaaa tcttattttg caaattatat aataaagatt
     9721 atgataattc aatatttagt ttaaatttaa aagaagcacc ggcatctaag cggcacttct
     9781 atagtttctt catttttatt ttgactattt attaaaatct aattgagaat aggtcaatac
     9841 ctgaaattta tcactatgcc ggtcaataaa cataataatc tgttttagca tatccttgtt
     9901 gtaggtcata ccaaattcat catcttcata tccgatttta tgaaatatta atacaaatgt
     9961 ttttaattca ttaattcctt gtttaagctg agctgtaact tcatttgctg aaatatttgt
    10021 tacgggattt attgaggtaa tgggataata gtagtaattg aaactatcta atacaattat
    10081 attatccgat gttctgacac ttctgtaccc tgcatttttc agttgcctta taaggtatgg
    10141 gttaatttct ccatgcggca tgacaaccat atcactgcat tttccaaaat atctgttgtc
    10201 catccaccgc cttgatttat caaagtctga aagaagttcg tcagtattgt tatatgcatc
    10261 tgccctgtgt gaataagaat gatttaataa atcccaacct tctaaatata attctgcaag
    10321 ttctttgtaa gacatatatt catcttcaac aattaatgaa ggaataatgg acacgctccc
    10381 cttataactg tattctttca ttaaactata agcttcagaa tatacactcc tccacccatc
    10441 atcaaacata aaaactattt tagttttgtc tctatcaatg gcagtattat tgttttgtat
    10501 gttgtaattt ttaagattca ttgctacgca aaaatctttg ccgctgttta tcaaaaatat
    10561 aattgttctt ggatatatta gtgcagtaat gataaaagat aataggatga tttttatttt
    10621 tatcttaatt ttctaccctc cgctctatta tagcaatttt atatttcttt aaaatgtgct
    10681 ttcaaatatg agttttgttt taaattaatt acaacagtat cttcttctga ctgaactacg
    10741 ccttcccagc ctgcgaatac aaaaccggga gatggtatag cttgaacctt tattggaata
    10801 tctgtaaagt aagtaccctc ccatagggta tctttatcta aaatatttac cgaattaatc
    10861 tttattgctc ctccctcatt catatcaact ttaattgaag ctgtaccggt taaaccaaag
    10921 taatttatta tgtgttgata aatgtgttga ggtctatacc ttgcgaattt cttcataact
    10981 tcaatctcat tactccaatt ttcaacctta ttattatgaa gcttccatct tgctatatga
    11041 tttgctatat tgggataata aatttcttcc atagcttcaa ttctgtttat tacaacatcc
    11101 ggtttataaa tagtgtttaa tctgtctgca aatgtattga taaactgatt cttaaagctt
    11161 tcattttcaa gtaaggacct caacaaaaaa gttgaccagg gtggattagg atagtagggt
    11221 ccatccgctt gtgttgccat ttcaagagaa ttgttttgaa cagggctttc acctttgaat
    11281 aatccaaatc cataatctaa atcaaataca agccatctcc atcgtccatc atgaccatag
    11341 agtgagttgg gctcataagc ttgtgttctt ttacgccata tgcgaacatt attgcccggc
    11401 cagtctctat tatcgcaata aatttgtaaa atattatatt caataaaatt atcaacatcc
    11461 attttagttt ttatatattc atagtccttc tgactctttg caccatgttc ttcaatatat
    11521 ttaatcatat ggttataatg catctcatct cccgcatatc cttcttgaat ttcaactcct
    11581 gcaatacctg tgggattatt tacaataact atatcttctg tgttcatatg ataataatta
    11641 cttatatagt ttttatcata tatttctctg atattatgaa ttccatagta ttctccattt
    11701 atatataata tcccttaata gctatgatgg atgctataaa attataagct attgtggtta
    11761 agaaataagc gaacatatac aaaggatagc cataaattat cttcataaat atcaaaatac
    11821 aattaatcac aaatacaata gtagcaaacg aattactcaa cagggcatct ataatcatta
    11881 ggaagcataa actattgtta aatacatttc taaacaagaa attatggttg tttataattg
    11941 catcaatgaa tcctttttgc cagcgtactc gctgattaat taatcctttc aaactttcag
    12001 gacattctgt ataacaggtg gcacttttat taaaaaccag ttttttatta tgagcctttg
    12061 catattcctg aaatcgtatg gttatatcaa tatcctcacc taagcctgat ctatacccgc
    12121 ctacttccat caaaaccttt cttctgaaaa caccaaaagc tcctgaaatt atacttaaag
    12181 catcattata tgcaagagag gctttgtaaa tataaaagcc ttttatataa tctaatgctt
    12241 gaattaaaac aatcggtttt tgtctttcat ctaatttaaa catttgcata atctgaacta
    12301 ctccgcctga tgcaattata tcttcatctt caaatatttt attcatgcta tctaaagcat
    12361 ttttctcaag gatacaatct ccatccatgg ttactatcag ttcgtattta gaaaataaaa
    12421 ttccggtatt taaactgctt gccttccctc cgttatattt atcaatcaca tatataaatg
    12481 aatacttatt agatttatat atacccttca tctggtctgt ccataaagat ataggttcag
    12541 cttctaaaac ttgtaaatct aaaatttcat tcaagatttc aaatgtatta tctaaagaac
    12601 catcattaat aaatattacc tcgaagttat cataatcaat atttaacaac ccttggatcg
    12661 tatatctgat tattaaagct tcattatagc agggaactaa aatagacatt cctttttgtg
    12721 gaacttggta aagagttcgt ctatctttga accttaaaga actgattaaa tgatatatcg
    12781 agaaaaatgt gcttaaaaaa agaactgcat aaaagaacac ttataaatcc cttccttctc
    12841 taaatgtcgg atttatattg gtagaaagaa tcttgaaatg gttaggaaat atagtgttgt
    12901 catcaggact tcccggccaa tttcctccaa cagcttgatt tataattata tacatatatt
    12961 catgaggcac tcccttggtt gtagtgtata tttgctgctc atctatatac caggttaaaa
    13021 actcagcgtc ccactttaag gcaacagtat attgttcttt ttctgaaacc atatgttcaa
    13081 aatagtccct ccctcgcatg ttgttttctt caaaatgaac tactccataa aatttatttg
    13141 gctcacttcc tatcatttca aatatatcta tttcaggcag cggacctccg cttatgggca
    13201 aaagccatat agccgggaac agcccttttc ccttagatat ctcaatttca aactcaaaat
    13261 atccatattt gaatgcttca gtactttcca ccagtcctga agtgtatatc ttatcatcta
    13321 taatttcttt ttttgatata attacaatgc tatcttcatc aaaagttaca ttatctctgc
    13381 tgtataattg cagctcattg ttataactgt cttttctatc aatggctatc caactatgtt
    13441 tattctcatc aatttcattt agctctatat caggtgtgtt gtatacgggt ttttgtccat
    13501 taatactact gtttacactt tgtttttttg tatattctcc tcttatttca tgaacgcttt
    13561 ttttaaattc cttgataatt acaagtaata ttgttactaa aaatagggtt gtcaataaaa
    13621 ttttaatgta tcttttaatc ataaaggtac ccctcttacc ttctaagaga aaagactaaa
    13681 attaaattaa ttacttaata tcttaatcat atatatgtga agatatcatt aatttatgac
    13741 tgatattttt agggaagttt ttagggtttt aacaaaaaaa gagacggata tgattaattt
    13801 aatcaaaaat accgtctcta aagtttacta aagattatta aaacatataa ttctcaagaa
    13861 cgaaaaatac cgtccccaaa attcttgtta atataattta aattatgtaa atcttatttt
    13921 gcaaattata tattaaagct tatgataatt caatatttag tttatgcttt tcatttttag
    13981 agttggattt tgtctaattt tgtattatac taattttggt ggtgacataa tgattaataa
    14041 gacgatatct ttaatattag ctctaatgtt aatggcaact cctcatgtaa aatgtcccga
    14101 agtaataaaa attctgtcta tcggcaattc tttttcacaa gattgtgttt attatttgta
    14161 tgatatagca cagtccgcag gtgttaatgt aataatcggt aacttgtacc acagcggcag
    14221 ctctttggaa agacacgata agaatgctaa ggaaaatctt aaggcgtatt cctatcaaaa
    14281 gtggacatct tcagaaatgg tagaagaaaa aaacaaaaca atgaaagaag tgctgctaga
    14341 tgaagactgg gattacataa catttcaaca atcttcggaa aattcgggat tatacgatac
    14401 ttatcagcct tacttgaata atttgattaa ttatgttaaa tcaatttcaa aaaatcctga
    14461 tgttaaattt gctctaaata tgacttgggc ttattcaaca agaagcagca ataaaggatt
    14521 tgcctattat aactatagcc aattcaatat gtaccgctgc attacagaat cctacaaacg
    14581 agcaattgag gaaacagaaa ttgatataat aattccatgc ggtacggcaa tacaaaatgc
    14641 tcggacaaac gaacacctca attcaattgg taaccagctc accaatgacg gctatcattt
    14701 agaaaatggt ataggaaaat atattgcagg attaactttc tttgaaaccc tggttattaa
    14761 tgataagaat ttagacttga atatttatga tgatgtgaca tttatacctg aaacaaaaaa
    14821 tgaaagagcc gatttagctg acatagcgaa aaagtccgta ataaaagcag taaattatcc
    14881 atatataata acaaaaataa gggctgacta ataagtcagc tcttactgat taaggtatat
    14941 cacattgtca t
//
LOCUS       DAOSHP010000039        14777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3332_length_14777_cov_8.695286, whole genome shotgun sequence.
ACCESSION   DAOSHP010000039 DAOSHP010000000
VERSION     DAOSHP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_14777_cov_8.695286"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..407
                     /locus_tag="PLB33_05310"
     CDS             141..407
                     /locus_tag="PLB33_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOG62975.1"
                     /translation="MLLNYMEEIVSRYLEKILKDPAYASICKCDYCKDDIKAMALNNL
                     NPFYITTKRGEVYAEYLSYEIQHQAEIIKEVIKATEFVSLHPNH"
     gene            complement(597..1079)
                     /locus_tag="PLB33_05315"
     CDS             complement(597..1079)
                     /locus_tag="PLB33_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOG62976.1"
                     /translation="MRTIVTVGISDMKIVKEHEILISYALGSCVGICIIDCVAQIAGM
                     AHIMLSYNKNGDKKEIFKYADTGITEMVRQMEKLGGIKSRMTAKIAGGAKMFDIKGSS
                     TIGSIGEKNIAAVKDTLQKLRIRLIAEDVGQNYGRTIMFDSTNGNLTIKSFGKEEKVI
                     "
     gene            complement(1076..1681)
                     /locus_tag="PLB33_05320"
     CDS             complement(1076..1681)
                     /locus_tag="PLB33_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOG62977.1"
                     /translation="MKLNESQMDVLKEIGNIGAGNAATSLSNMLSKRIEMNVPEVSLL
                     DYNDIIDSIGGAESIVVGVLIGFEGDIDGVILFLLKKEFVHLILNSLIGTELKNFEEV
                     SEIEMSALSEISNIMVSSYVNSISSLTNMNINVTVPSLNIDMSGALLDSVAAEFSEIA
                     DKAVFIKEKYYCMNETVYSYMLLLPSMPSLKILLNKFGIYT"
     gene            complement(1746..>1994)
                     /locus_tag="PLB33_05325"
     CDS             complement(1746..>1994)
                     /locus_tag="PLB33_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG62978.1"
                     /translation="LMLMKIKINNEISEIKKQLQEEIPSDNITEHRIICKGIIYSNTR
                     ITIGWLKYRVRQDISYSKIYNDGNEIVILPLNPSDIEP"
     assembly_gap    1995..2094
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2095..3328)
                     /locus_tag="PLB33_05330"
     CDS             complement(<2095..3328)
                     /locus_tag="PLB33_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOG62979.1"
                     /translation="MDKNQQPSYKINHLVSANKLKAYIRIELLDKSEEVEISCDEIFE
                     YLKEQEITYGILENDIIEFCKNKEYAKELIAAGGKEPVNGKDAEIIYNFDISNEKKFA
                     ESDDGTIDFRNLNNVTNVKKDTVLCYIVPASEGEDGIDVYGNPISYKKGRDISFNYGK
                     NTYISEDGLQLLASTDGCVEFKNNKVYVEDVYKVNNVDNSTGNIDFVGSVIINGDVKE
                     GFSVTAAGDIKIRGMVEGAYIKSDGDVVISKGMNGMSKGSIYAKGNITSKYIENATIV
                     SEKNIYAEALINSDVKAGDSIIIKGLISSIIGGTTTAKNKIYAKTIGNKTNPETNIII
                     DLTEYIEEQKEIEKKIRMNRRLKEELSEKNNKIKEIDEKYELIINSSLDAENKNSVQK
                     QLMLMKIKINNEISEIKKQ"
     gene            complement(3360..>3662)
                     /locus_tag="PLB33_05335"
     CDS             complement(3360..>3662)
                     /locus_tag="PLB33_05335"
                     /inference="COORDINATES: protein motif:HMM:NF018168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOG62980.1"
                     /translation="EEPIIIKNSDDIDSIEIGVFTFNNNADLEQAGNNLYRISNEDAE
                     FNIAEAKVLKGYLEASNVEISDEMVKLIQIQRAFQLNSTVIKTADEIEQTINTLRG"
     assembly_gap    3663..3762
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3861..4613)
                     /locus_tag="PLB33_05340"
     CDS             complement(3861..4613)
                     /locus_tag="PLB33_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOG62981.1"
                     /translation="MSRGFYTLASGMITQQRKIDVSSNNVANLNTAGYKKEQAITSNF
                     GSLLINKYKQNGIKEEAEPINNISLIRAVTENNTIHSQGVLEETGNITDFAIIGAGFF
                     AIDNEGQILYTRNGSFNIDEEGYLFLEGIGRVQGEFGDIYVEGDKFEFLEDGSIIIEG
                     EIADKIAVFDFPDYNDLNKYGEGTFISDVEPDLAEYPIIKNKTIERSNVEVTEELTNI
                     ISSQRALQTAAQALKIYDSINDKAVNEIGKVQ"
     gene            complement(4637..5419)
                     /locus_tag="PLB33_05345"
     CDS             complement(4637..5419)
                     /locus_tag="PLB33_05345"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOG62982.1"
                     /translation="MILTENKQLQIEEYQNHLWEQYHATGDLTIRNQIIERYIYLVRI
                     IALKMRGIYQQYGDVEDIVNEGIIALMDAIEKFDISKNTKFETYASIRIKGSIIDYVR
                     KQDWIPRKVKNDYKIIKESEELLSNKLGRTPTDNEIVEHLNMDINDYNKIISNAYGTS
                     ILSFEELIEEANLSEYGLKDRHKIPEHEIESKELYKILVESVNKLNEKEKLIISLYYK
                     EELKLKDIAQVLEISNSRVSQIHTSALQKLEKSVRNYLYSDN"
     gene            complement(5436..7478)
                     /gene="flhA"
                     /locus_tag="PLB33_05350"
     CDS             complement(5436..7478)
                     /gene="flhA"
                     /locus_tag="PLB33_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOG62983.1"
                     /translation="MRSIFKNSISIFIIAVILMIIVPLPPFFLDLMFIINIAFAIVIL
                     LTTMYVREALEFSVFPSLLLITTLFRLSLNVSSTRLILTNNGHAGAVIATFGSFVLQG
                     NAVVGFIIFLIIVLVQFIVITKGAERVAEVTARFTLDAMPGKQMAIDADLSSGIITEQ
                     EAMQRRKGVQRESDFYGAMDGATKFVKGDAIMAIVITFINFVGGVIIGMVQGGDTFAG
                     VLQTYSIATVGDGLVSQIPALLISTATSMIVTRAASESSLNVDLTKQIRSQPVVLIIS
                     GVVILFMVFIPGFPLPQVITLSSLLIFLGISLLRARVSAAAETDKDKEEEPVSEEEYF
                     RNIDNIYELIQIDPIAMEVGYSLIPLVDVDGGNFIDRVVMFRRQFAQDMGMVVPSVRL
                     RDNGYLNPNEYVIKVKGEEVAKGEILVEYYLALDPGNLTGEVDGIETIEPAYGIPSKW
                     IKKDKKELAEIYGYTVIDPLSVLITHLSEVVKTHAHELLSRQDLNQLLDKIKKDNKLL
                     VEEVVPGIVSPGNLQKILGNLLKEGIPIKDMETILETISDYGSSVKDTEMLTEYVRQA
                     LKRTITRKWSEGGQIRVITLSTDVEKLIINSISKNDKGSYLSIDPELMQKLVTQLIEQ
                     INKLKGEINIPIILTSPFVRGYFRKLLDQFYPNSVVLSFNEIDNSVQIQALGNVAI"
     gene            complement(7482..8543)
                     /gene="flhB"
                     /locus_tag="PLB33_05355"
     CDS             complement(7482..8543)
                     /gene="flhB"
                     /locus_tag="PLB33_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOG62984.1"
                     /translation="MAGENKTEKASPKKRRDERKKGNVFQSKDVVTVGVIAISFLILN
                     LWAPYIYKYTTNVFYKYVILTKNTYTINDNLIAQAFRDITFTIFASAGLVMFFVITTT
                     VVLTGAQTKFLVSKENIKFKFSKLNPLSGIKRMFSIRSLVEVIKNLIKISILAYVIYS
                     SIANNLFQVPRLISVNLAAGINFIRTSVMSIVKSVVIAFIVISAFDFLYSWWEYEKSI
                     RMTKQEVKEEYKQLEGDPKIRGIIKEKQRAFSMTRMMQQVPKADVIIRNPTHYAVAIS
                     YNIEKDNAPLVLAKGKDRMAARIIEKAEEYKINIIENIPLARALYAESEINREIPVKY
                     YEDVAEILAWVYRLKEKGI"
     gene            complement(8553..9323)
                     /gene="fliR"
                     /locus_tag="PLB33_05360"
     CDS             complement(8553..9323)
                     /gene="fliR"
                     /locus_tag="PLB33_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOG62985.1"
                     /translation="MTFSGDFTYFLLIFARMSGCVFFNQILGRGNLPNIFKISLSLFL
                     AVTTYYILPPEDDMVITTTIEFILLIIKELFIGYLIGYIISLFFSTVVISGEVIGIQI
                     GMSMAKIYDPTRSVSMGIMGSFLNLILILIFFSANGHLNLIKIIITTGKLIEVGNFSI
                     PTHLFYNMVELFQQILILALKLSMPIMAVEIILESGIGILMKAIPQIQVFSVNVQLKI
                     LVGIFLIFILVPTFSTFIENVIVLMFDTVENSLSALIT"
     gene            complement(9336..9602)
                     /gene="fliQ"
                     /locus_tag="PLB33_05365"
     CDS             complement(9336..9602)
                     /gene="fliQ"
                     /locus_tag="PLB33_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOG62986.1"
                     /translation="MSISQVMDVMQAAAGAAIKVGAPILIVSLIIGLSIAVFQAATQI
                     HEQTLTFVPKLLVTAIMLIVLGNWMTEVLSDFTYYVFDLMLRVY"
     gene            complement(9642..10328)
                     /gene="fliP"
                     /locus_tag="PLB33_05370"
     CDS             complement(9642..10328)
                     /gene="fliP"
                     /locus_tag="PLB33_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443404.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOG62987.1"
                     /translation="MNTQAVYGGVINMDINGNSVETLEILFLLTIIALLPSILIMMSS
                     FTRIIIVLSFVKNALGLQQTPPNQVLIGIAIFLTLFTMSPVMEEINTSAYEPYKNEEI
                     SQEEFLDTASIPIKTWMLKQTGSNELQMFLDMSGDEAYKETENIEELPLTIIIPSFIT
                     SELKRAFIIGFLLYIPFLIIDMIVSSVLMSMGMMMLPPVMISMPFKILLFVVVDGWSL
                     LFKTLITTFN"
     gene            complement(10363..10725)
                     /locus_tag="PLB33_05375"
     CDS             complement(10363..10725)
                     /locus_tag="PLB33_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOG62988.1"
                     /translation="MIIINKDIISLIISIIGIIIVLYLTYYCTKWLSTKTTIAAKSRY
                     MNVADKLMLGPNNFITIVEINNKYYLIGITEKDINIIKELEDFHHLPDDKESSNTFNN
                     ILNKYKKTFIGSNNDKKL"
     gene            complement(10722..11090)
                     /locus_tag="PLB33_05380"
     CDS             complement(10722..11090)
                     /locus_tag="PLB33_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG62989.1"
                     /translation="MEGKILIVDDAAFMRMMIKDTLKSSGYENIIEAADGEQAIQTYK
                     NEKPDLVIMDITMPNKNGLDALKEIRKMDPNAVIVMCSAMGQESMVVEAIRNGAKDFI
                     VKPFKSERIIKTVQSIIDRG"
     assembly_gap    11136..11235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11256..12353)
                     /gene="fliY"
                     /locus_tag="PLB33_05385"
     CDS             complement(11256..12353)
                     /gene="fliY"
                     /locus_tag="PLB33_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HOG62990.1"
                     /translation="MLNRKIFSNMERDVIGEVMNISLGSSATSLSSILGKRVEITVPH
                     INVTTAQEFSYKQLEPAIGVEINYIEGLKGVNVMIWKRSDAKIILEALLNKKIPDEEF
                     EMDEISISAICEVMNQMMGSAATALSEFLGKHINISTPIAFEIDDNNEFKSKYFAEDD
                     EIVVVSFDLSIEGELQSQFINVMEVSLAKDIVSNFLRETEKSTSNEIQIEENNNIEKE
                     DAVNKKIDTIIKQNNDYEEIIKRERMKTNQNQKEKEETFVNVRPVEYESFDDEEEVLS
                     EEQLSNLELIMMVPLTISVEIGKTSKQIKDILEISPGTIIELDKQAGALVDIIVNGKL
                     IAKGEVVVVNDNFGVRVVEILKKDELMKITL"
     gene            complement(12365..13345)
                     /gene="fliM"
                     /locus_tag="PLB33_05390"
     CDS             complement(12365..13345)
                     /gene="fliM"
                     /locus_tag="PLB33_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOG62991.1"
                     /translation="MAEVLSQSQIDELLNGLSTGDVSIQELEENEKRIKEYDFRSPKK
                     ITKETIRLLKGIYENYCRMLTSRLTSMLRLMCDVTVEQVEESIYHEYNNALEDYVLMG
                     LINVKYPDNETSDSQILMEMSRPLSFVIIDRLLGGIGEEYGYIRDYTDIELSLLSNVL
                     NQLVILMGTTWENTLNIETELEKVETNSRFIQSINYNDTVVIIILNVVLNELSGKITL
                     CIPSNIIDEILKGANIYSKNISKKVNIEEQKTAIMNSLKSSYLTITGVLGSTQATLKD
                     ILSIKPGDLIVLDRKINSNVDIKVDNEKWFEGKWGTRKNKGVIKINKTIY"
     gene            complement(13373..14182)
                     /locus_tag="PLB33_05395"
     CDS             complement(13373..14182)
                     /locus_tag="PLB33_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOG62992.1"
                     /translation="MARRKKREESGGASWLETYSDMVTLLLTFFIMLYAMSTIDAQKF
                     AAIVASFSKEMNPEAYYQMMEAEAAEQETVSSYEEQEEPEEIEDIDVLYTLLKQYIDE
                     NELAGQVEISKSEEYVFIRFSDSVTFRGYSDILEQKGKGVLDVLAEGLAMVDEYIEEV
                     VIAGHTAKVEYDMTDIDRSLSTNRANVVLRYLENKDVIDPAKYLAIGYGLYSPIADNS
                     TAEGRAKNRRVEIYISRKGHPISYTRIIQETINNGKTDNNDLEYRRDIYIE"
     gene            complement(14205..>14777)
                     /locus_tag="PLB33_05400"
     CDS             complement(14205..>14777)
                     /locus_tag="PLB33_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOG62993.1"
                     /translation="LLVLEEKVNQMDLQDEFLKYCVMLIVDAIEPTKVKAQIENEIDC
                     IEARHASAWKVFDTLGSFGPAFGMLGTLIGLINMLANMDAGEGADALGKGMSVALVTT
                     FYGSVLTNMICAPISNRLKAKHDEEMIAKELIMEGVLSIQSGENPKYIREKLNSYVTY
                     QERSTMAGAEGQGKSKKRSKRNGKEKEANA"
BASE COUNT         4098 a         2853 c         1992 g         5534 t
ORIGIN      
        1 ttggcatata tcaattcctt gtacaataaa tactacaatt ttaaagacat cgacaaccat
       61 aaatactatt tctacattat tatatccgta attggtatgt atagagaagt aatcgattaa
      121 ttatttgaag gagtacgaaa atgttgctga attatatgga agaaatcgtt agcagatatc
      181 ttgaaaagat tttaaaagac cccgcgtatg catctatatg caaatgcgat tattgcaaag
      241 atgatataaa agctatggcc ttaaacaatc tgaatccgtt ttacattacc actaaaagag
      301 gtgaagtgta cgcagaatac ttatcttatg aaatccaaca tcaagcagaa ataatcaaag
      361 aagtaataaa ggcaacagaa tttgtttcat tgcacccaaa ccactaagat gctctgtatt
      421 tcacccagat aaagtctgta tcatcctgag tgcatgtgaa tgattttata ccctttgtca
      481 tcctgagcgc aagggaagga tctcatgaga ttcttcgagc taaagctctc agaatgacag
      541 tctgcgggtt actcatagcg atgacagttt gtgtttattc ataacaatga cactttctaa
      601 ataacctttt cttcctttcc gaatgattta attgttaaat tcccgttagt actgtcaaac
      661 attatggtgc ggccgtagtt ttgacccacg tcttcggcaa taagtcttat tcttaacttt
      721 tgcaatgtat cttttacagc agctatattc ttttcgccga tgctcccgat tgttgagctt
      781 cctttgatat cgaacatttt agcgccgcct gcaatttttg ccgtcattct tgattttatg
      841 ccgcctaact tttccatttg tcttaccatt tctgtaatac ctgtgtctgc atatttaaaa
      901 atttcttttt tatcgccatt tttattataa gataacataa tatgtgccat tcctgctatt
      961 tgtgcaacgc aatcgattat gcaaatgccg acacaggagc cgagagcata tgatattagt
     1021 atttcatgct ccttaactat tttcatgtct gaaattccta cggttacaat tgttctcatg
     1081 tatatattcc aaatttgttt aatagaattt ttaaggatgg catgcttggc aacaaaagca
     1141 tataactgta tactgtttca ttcatgcaat aatatttttc tttaatgaag acagccttgt
     1201 ctgcaatttc cgaaaattct gcggcaacgg aatccagcag ggcacctgac atatcgatat
     1261 tcagcgacgg aacagtaaca ttaatattca tatttgtaag agaagatatt gaattcacat
     1321 aggaagatac cattatattg cttatttcag acaatgcaga catttctatt tcagatactt
     1381 cttcaaaatt tttaagctct gtcccaatca atgaattcaa gattaaatga acaaattcct
     1441 tttttaacaa aaatagtatt acaccatcaa tatctccttc gaaaccaatc agtacaccga
     1501 caactatact ttcggctccg cctatggaat ctattatatc attgtaatct aataaactta
     1561 cttcaggcac attcatttct attcttttag aaagcatatt ggaaagggat gtcgctgcat
     1621 tgcctgcacc gatatttcct atctccttta gcacatccat ttgactttca tttaatttca
     1681 tgtctttctc ccttatgtaa gatttttgtg ctgatggaat tttgtccccc cttataaaaa
     1741 ttccatcatg gctcaatatc cgaaggatta agcggaagaa ttacaatttc gtttccgtca
     1801 ttatatattt tactgtagct tatatcctgt cttactctat actttaacca gcctatggtt
     1861 attcttgtat tggaataaat tataccctta catataattc tatgttctgt tatattatcg
     1921 gaaggtattt cttcttgaag ctgctttttt atttcagaaa tttcattgtt tatttttatt
     1981 ttcataagca ttaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngctgct
     2101 tttttatttc agaaatttca ttgtttattt ttattttcat aagcattaat tgcttctgca
     2161 cggaattttt attttcggca tctaaggatg aattaataat taattcatat ttttcgtcta
     2221 tttcttttat tttattgttc ttttccgata actcttcttt cagacggcga ttcatcctga
     2281 tttttttttc tatttccttt tgttcttcta tatattctgt taaatcaatt attatatttg
     2341 tttcgggatt tgttttattg cctatagtct tggcgtaaat tttatttttt gctgtggttg
     2401 ttcctccgat aatagaagaa atcagtcctt ttattataat agagtctcct gcttttacat
     2461 cgctgtttat cagcgcttct gcgtaaatat ttttttcgga tactatggtt gcattttcaa
     2521 tatacttgct tgttatattt cctttcgcat aaatgctgcc tttgctcatg ccattcatac
     2581 ccttgctgat aactacgtca ccgtcagatt ttatatatgc accttccacc atacccctta
     2641 ttttaatatc tcctgcagca gttaccgaaa atccttcttt tacatctcca tttataatta
     2701 cgcttcctac aaaatcaata ttgcctgttg aattatccac attattaact ttataaacat
     2761 cttctacata tactttatta tttttaaatt ctacacatcc gtcggtgctt gccaaaagtt
     2821 gcaggccgtc ttctgaaata tatgtatttt tcccgtaatt aaatgatata tctcttcctt
     2881 ttttatagct gatgggattt ccatatacat ctattccgtc ttctccctct gaggcaggaa
     2941 ctatatagca taaaaccgta tcctttttta cgtttgttac attatttaag tttctaaaat
     3001 caatagtccc gtcatcgctt tcagcaaact ttttttcatt tgaaatatca aaattataaa
     3061 ttatctctgc atctttcccg tttacaggct ccttcccgcc ggcagcaata agctcctttg
     3121 catattcctt atttttgcaa aattcaatta tatcattttc taaaattcca tatgtaattt
     3181 cttgctcttt taagtattca aaaatttcgt cacaggaaat ttcaacttcc tctgacttat
     3241 caagaagttc aattcttata tatgccttca gtttgtttgc tgataccaga tgattgattt
     3301 tgtatgaggg ttgctggttt ttatccatga ctcctcctta ttttgatatt tctccaatct
     3361 tatcccctta atgtattaat ggtttgttca atttcgtcgg cagtttttat aacggttgaa
     3421 ttaagctgaa atgctctttg aatttgaatt aactttacca tttcatcaga tatttcaaca
     3481 ttggaagctt ccaggtaacc cttcaaaacc tttgcttcag ctatgttaaa ctctgcatct
     3541 tcatttgaaa ttctgtatag gttattacct gcctgctcta aatcagcatt attgttaaaa
     3601 gtaaaaaccc ctatctctat actgtctata tcgtcagaat ttttaataat tataggctcc
     3661 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtatgtt tgccatattg
     3781 tttgatataa catccaaata atttttattg cttattgctc cgtttgttgc cgtataatat
     3841 gctttaatca tataggcctc ctattgtacc tttcctattt cattgactgc tttgtcattt
     3901 atcgaatcat atatttttag cgcttgagct gctgtttgca aagccctttg tgatgaaatt
     3961 atatttgtta attcttccgt aacttcaaca ttagaccttt caatagtttt attctttatg
     4021 attggatatt ctgctaagtc cggctctaca tccgaaataa acgttccttc gccatatttg
     4081 tttaaatcat tgtaatcagg gaagtcgaaa acagcaattt tgtctgcaat ttcaccttcg
     4141 attattatac ttccgtcttc cagaaattca aatttatcac cttctacata tatatctcca
     4201 aattccccct gtactcttcc aataccttca agaaacaaat atccttcttc atcaatgtta
     4261 aagcttccgt tacgggtgta taaaatctgc ccctcattat caattgcaaa gaatcctgct
     4321 ccaataattg caaaatccgt aatatttcct gtttcctcca aaaccccctg cgaatgtatg
     4381 gtattgtttt ccgttacggc tctaattaga gaaatattgt tgattggctc tgcttcttct
     4441 ttaattccat tttgcttgta tttatttatt agtaagctgc cgaaatttga agttatggct
     4501 tgttcctttt tataacctgc cgtatttaag tttgctacat tattgcttga tacatcgatt
     4561 tttctttgct gagtaatcat acctgaagct aatgtataaa atcctcttga catattgcac
     4621 ctctcatctt tcatttttaa ttatcggaat ataaatagtt tcttacgctt ttttctaact
     4681 tctgtaatgc cgatgtatgt atttgtgata cccttgaatt gcttatttcc aatacttgcg
     4741 ctatatcctt aagctttaat tcttccttgt aatataaaga aattataagc ttttcctttt
     4801 cattaagttt atttacgctt tccaccagta ttttatataa ttcctttgat tcgatttcat
     4861 gctcaggaat cttatgcctg tcttttaatc cgtactcgga taaattagct tcctcaatca
     4921 attcctcaaa ggataatatg cttgtaccat atgcattgct gataattttg ttgtaatcgt
     4981 ttatgtccat attaagatgt tccactattt cattgtccgt gggagttctg cctaacttat
     5041 tggacaagag ctcttctgat tcttttatta ttttgtaatc atttttaact tttcgcggta
     5101 tccagtcttg tttccttaca tagtcgataa tcgacccttt aatccttatt gaagcatatg
     5161 tttcaaattt cgtatttttg gatatatcaa atttctcgat tgcatccatc agagctatga
     5221 ttccttcatt tacaatatcc tcaacatccc cgtactgttg atatatgccc ctcattttca
     5281 gggctattat tcttactaag tagatgtatc tttcaataat ttgatttctg atggttaaat
     5341 ctccggtagc atggtactgc tcccataagt ggttttgata ttcttcaatt tgtaattgtt
     5401 tgttttcggt taaaatcata tcatcactct ttcatttata ttgctacatt gcccaaggcc
     5461 tgtatttgta ctgaattgtc aatttcatta aatgataata caacggagtt aggataaaat
     5521 tggtctaata gctttctgaa atatcctctt acaaatggtg aagtaagaat tatcgggatg
     5581 tttatttcac ctttcagctt attgatttgc tcaatcagct gcgtcactaa tttttgcatc
     5641 aattcaggat caattgataa ataggagcct ttatcatttt tacttattga attgattatt
     5701 aatttttcaa catcagtgct taatgtaatg accctgattt gaccgccctc agaccatttg
     5761 cgtgttatcg ttctttttag tgcttgtctt acgtattccg taagcatttc cgtatccttg
     5821 acagaagacc cgtaatcact gattgtttct aaaattgttt ccatatcttt aataggaatg
     5881 ccttctttaa gcaaattgcc caatattttt tgcagattgc ccggagatac gattccggga
     5941 acaacttctt ccaccaataa tttattatcc ttctttattt tgtctagcag ctggtttaaa
     6001 tcctgtcttg ataataattc atgagcatgt gtttttacta cctccgacaa atgtgttatt
     6061 aatacagaca aaggatctat tacggtatat ccgtaaattt ccgccaactc ttttttatcc
     6121 ttcttaatcc atttgcttgg tattccatat gcaggctcta ttgtttcaat accatccact
     6181 tctcccgtta agtttccggg gtctaaggcc aagtagtatt ctactaatat ttcccccttg
     6241 gcaacttctt ctcctttaac ttttataaca tactcgtttg gattaagata tccgttatct
     6301 ctcaatctga ccgaaggaac taccattccc atatcttggg caaattgacg cctgaacatt
     6361 actactctgt caatgaagtt tcctccgtca acatctacca gcggaattaa actgtatcct
     6421 acttccattg caatagggtc tatttgaatt aattcataaa tattatcaat atttctgaaa
     6481 tactcctctt ccgatacagg ctcttcctct ttatccttat ctgtttcagc cgcagcggaa
     6541 actcttgctc ttaacaggct gattcctaaa aaaatcagca gtgatgacag tgtaattacc
     6601 tgaggcaaag gaaatcccgg tataaagacc ataaataata ttactactcc ggatattatc
     6661 aagactaccg gctgtgacct tatttgtttg gtcaaatcta cgtttaagct tgactcggaa
     6721 gcagctcttg taacaatcat gcttgttgcc gtagatatta gtagagcagg tatctgagaa
     6781 accagtccgt cacctacggt tgctatggaa taagtctgaa gtactccggc gaaagtgtcg
     6841 cctccctgaa ccatacctat gatgacaccg cctacaaaat ttataaaggt aattactatt
     6901 gccattatag cgtcgccctt tacaaatttt gttgcaccat ccattgctcc ataaaaatcc
     6961 gattcccttt ggactccttt acgcctctgc attgcttcct gctctgtgat tatgcctgag
     7021 cttaaatcag catcaatggc catttgtttt ccgggcattg catccaaggt aaatcttgcg
     7081 gtaacttctg ctactctttc ggcgcccttt gtaattacta taaattgaac taagactata
     7141 attaaaaata taataaaccc gacaactgca tttccttgta atacaaagct tccgaatgtg
     7201 gctataactg cccctgcatg tccattgttt gttaagatca accttgtgga agatacattt
     7261 aaggacagtc tgaatagggt agttattaaa agaagcgaag gaaatacgga aaattccaat
     7321 gcttccctta cgtacatcgt tgtgagcaat ataactatgg caaatgcaat attgataata
     7381 aacattaaat ccaaaaagaa gggaggtaat ggtactatta tcataagtat tactgctata
     7441 ataaatattg aaatagaatt cttgaatata cttctcatat cttatattcc tttttcctta
     7501 agcctataaa cccaagctaa aatttctgca acatcttcat aatattttac gggtatttcc
     7561 cgatttattt ctgactcggc ataaagagcc cttgccaatg gaatgttttc aataatgtta
     7621 attttatatt cctctgcctt ctcaataatc cttgctgcca ttctgtcttt tccttttgcc
     7681 agtactaggg gagcattgtc tttttcaatg ttataactta tagcaacagc ataatgtgta
     7741 gggtttctta taataacatc ggcttttggt acttgctgca tcattcttgt cattgaaaag
     7801 gctctttgtt tttcttttat tattcctctt attttggggt caccctctaa ttgcttatac
     7861 tcttccttaa cttcttgctt ggtcattctg atactttttt catattccca ccaggaatat
     7921 aaaaagtcga atgcagaaat aactataaaa gcaattacaa cactttttac tatggacata
     7981 accgatgttc ttatgaaatt aattccggca gctagattga ctgaaatcaa tctgggtacc
     8041 tgaaataaat tgtttgcaat cgaagaatat attacatatg ctaatattga tattttaatt
     8101 aaattcttta ttacctccac aagagaacgg atagaaaaca tcctttttat gccggaaagc
     8161 ggatttaatt ttgaaaactt aaattttata ttttccttag aaactaaaaa cttagtttgt
     8221 gcaccggtta atacaacagt cgttgttatt acaaaaaaca ttacgagtcc cgctgatgca
     8281 aatatggtaa aggtaatatc cctgaacgct tgagcaataa gattatcatt tattgtataa
     8341 gtattttttg tcaggatcac gtatttataa aatacatttg tagtatactt ataaatatat
     8401 ggggcccata gattcaatat caaaaatgat attgcaatta ctccaacggt tactacatcc
     8461 ttgctctgaa atacattacc tttttttctt tcatcccgtc tttttttggg ggaagctttt
     8521 tcggttttat tttcgcctgc catcacttca ccttatgtaa ttaatgcgct tagactgttt
     8581 tctacagtat caaacatcaa tacgattaca ttttctataa atgttgaaaa tgtaggtaca
     8641 agtataaata ttaagaatat tcccactaat attttcaact gaacatttac ggaaaaaacc
     8701 tgtatttgcg gtattgcctt catcaaaatt ccaattcccg actccaatat tatttctacc
     8761 gccattatgg gcattgacag ttttaatgcc agtattaata tttgttgaaa cagttcgacc
     8821 atattgtaaa acagatgagt cggtatggaa aaattgccta cttctattaa tttgccggtc
     8881 gttataataa tttttatcaa atttaaatga ccgtttgcac tgaaaaagat aagtattaaa
     8941 attagattta agaagcttcc cataatgccc atagaaacac ttcttgtggg gtcatatatt
     9001 ttagccatgg acattcctat ttgtattcct ataacttcac cgcttataac aaccgtactg
     9061 aaaaataagc ttattatata acctatcaaa taaccgataa acaactcttt tataattaat
     9121 aatataaatt caattgtagt tgtaatgacc atgtcatctt caggaggcag tatataatag
     9181 gttgttacag ccagaaataa gcttaaggat attttaaata tattcggtaa attccctctg
     9241 cccaatattt gattaaaaaa aacacaaccg ctcattcttg caaatattaa taaaaaataa
     9301 gtaaaatctc ctgaaaatgt catcgcaagt cctcattaat atactcttaa cattaaatca
     9361 aatacatagt atgtaaaatc agacaaaact tcagtcatcc aatttcccaa tacgattaac
     9421 attattgcag taaccaataa ttttggaaca aaggtcaatg tctgctcatg tatttgagtt
     9481 gcagcttgaa aaacagctat tgataaacct attattaaac ttactatcag tatgggtgcc
     9541 cctactttta ttgcagctcc cgcagcagcc tgcataacat ccataacctg cgaaatgctc
     9601 attattatct ccttctctta atcaggtatt ttaaatacct attaattaaa cgtcgtaatt
     9661 aatgtcttaa ataataaaga ccacccgtcc acaacaacaa ataacagtat tttaaacggc
     9721 attgatatca ttaccggcgg cagcatcatc atgcccatgg acataagaac gcttgaaact
     9781 atcatatcta ttattaagaa tgggatataa agtaaaaatc ctattataaa tgctcttttt
     9841 aattcactgg taataaagga cggaataatt attgtcagag gaagttcttc gatattttcg
     9901 gtttctttat aagcttcatc tcccgacata tccaaaaaca tttgtaattc attgctgcct
     9961 gtttgtttca gcatccatgt ttttatcggg attgaagccg tatctaaaaa ttcctcctgt
    10021 gaaatttctt catttttata tggctcataa gcagatgtgt ttatttcttc cattacgggt
    10081 gacattgtaa ataatgtcaa gaatattgct atgcctatca gtacttgatt aggcggcgtc
    10141 tgctgcaatc cgagtgcatt ttttacaaat gataatacga taattattct tgtaaaagat
    10201 gacatcatta ttaagattga cgggagtaaa gcaataattg tcaaaagaaa cagaatttcc
    10261 aatgtctcta ctgaattccc gtttatatcc atattaataa ctccgccgta aacagcctga
    10321 gtattcataa gcattaatat taagactata aaaattgatt tattataact tcttgtcatt
    10381 gttgcttcct ataaatgttt ttttatattt gttcaaaata ttattgaatg tattactgct
    10441 ttctttatca tcgggtaaat ggtgaaaatc ttctaattct tttatgatat tgatatcttt
    10501 ttccgtaatg cctattaaat aatacttgtt gttaatttct actatagtaa taaaattgtt
    10561 cggacccagc attaatttgt ctgctacatt catatatctt gatttagcgg ctatggttgt
    10621 ctttgttgaa agccattttg tacaatagta ggtcaaatac aaaacaatta ttattcctat
    10681 aattgatata ataagtgaga ttatatcttt attaattata atcaacccct atcgattata
    10741 ctttgaacgg tttttataat tctttccgat ttaaagggtt taactataaa atccttagca
    10801 ccgtttctta tcgcttcgac aaccatggat tcttgtccca ttgccgagca cattactatt
    10861 acggcatttg ggtccatttt ccttatttcc ttcaatgcat ccaatccatt tttattgggc
    10921 atggttatat ccattattac caagtcaggt ttttcatttt tgtatgtttg aatagcctgc
    10981 tctccgtcag cagcttctat aatattttca taaccgctgc tttttaaggt gtcctttatc
    11041 atcattctca taaatgcagc atcatctact attaaaattt taccttccat tctatcctcc
    11101 gttgtatgcc atttctatgt aacatatgtg tctttnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnacaat ttttgaatat ttcaattata atgttatttt cattaattcg
    11281 tcttttttta atatttcaac tactcttact ccaaaattat catttaccac aacaacttca
    11341 ccttttgcaa ttaacttgcc gttaacaata atatctacta atgctccggc ctgtttatca
    11401 agctcaataa ttgttcccgg tgaaatttca agaatatcct ttatttgttt gctggttttt
    11461 ccgatttcaa cactgatagt caaaggaacc atcattatca attccaaatt agaaagctgc
    11521 tcttctgata atacttcctc ttcgtcatca aatgattcat attctacagg tcttacattt
    11581 acgaatgttt cctctttttc cttttgattt tgatttgttt tcatacgctc cctttttatt
    11641 atttcttcat aatcattatt ttgcttgatt atcgtgtcaa tttttttatt tacggcatct
    11701 tctttttcaa tattattgtt ttcttctatt tgtatttcgt tgcttgtact tttttcggtt
    11761 tcccgtaaaa aatttgatac tatatctttt gctaatgaaa cctccatgac attaataaat
    11821 tggctttgca attctccttc aatagataaa tcaaaactga ctacaacaat ttcatcatcc
    11881 tctgcaaagt atttactttt aaattcatta ttatcgtcaa tttcaaaagc aatcggtgtt
    11941 gatatgttta tgtgtttccc taagaactct gatagggctg tagcagctga ccccatcatc
    12001 tggttcatta cttcgcaaat ggcgcttatg ctgatttcat ccatttcaaa ttcttcatcc
    12061 ggtattttct tgtttagaag ggcttccaaa attattttgg catcagatct tttccatatc
    12121 attacattta cgccttttaa gccttctata tagtttattt caactcctat ggccggctct
    12181 aactgcttat agctaaactc ttgagcagtt gtaacattaa tatgcggaac ggttatttca
    12241 actcttttgc ctaatattga ggataatgaa gttgcagatg agcctaagct tatattcata
    12301 acttccccta ttacatctcg ttccatattt gaaaaaattt ttctatttag cataaataac
    12361 tcccttagta tatagtttta tttatcttaa ttactccctt gttcttcctt gttccccatt
    12421 tgccttcaaa ccacttttca ttatcaactt ttatatctac attcgaattt atttttctat
    12481 caagtacgat taaatctccc ggcttaatac tgagtatatc tttcaatgtt gcctgagtac
    12541 ttccgagcac tccggttata gttaagtatg atgattttaa tgaattcata attgctgttt
    12601 tttgctcttc aatattaact tttttactta tattctttga atagatattt gcacctttca
    12661 gtatttcgtc aataatgttt gaaggtatgc ataaggtgat tttccctgac agctcattta
    12721 atactacatt taatattatt attactacgg tatcattgta atttattgac tgaataaatc
    12781 tggagttggt ttcaactttt tctaactcgg tttctatatt taaagtgttt tcccatgttg
    12841 ttcccattag aataaccaac tgatttaata cattgctgag cagtgacagc tcaatatccg
    12901 tgtaatctct tatatatccg tattcttctc cgattcctcc aagcagcctg tctataataa
    12961 caaaggacaa aggtctggac atttccataa gaatttggct gtcagaagtt tcattatccg
    13021 gatattttac attgataaga cccattaata cataatcctc taaggcattg ttgtattcat
    13081 ggtatatgct ttcttccacc tgttctaccg ttacatcgca catcagcctg agcattgatg
    13141 ttaatcttga agtaagcatt ctgcagtagt tttcgtaaat accctttagc aatcttatgg
    13201 tttcctttgt gattttctta gggcttctga aatcatattc tttaattctt ttctcatttt
    13261 cctctaattc ttgtatactt acatcacctg tgctcaaacc gttaagcaac tcatctattt
    13321 gactttgtga tagtacttcc gccatattta atcctcattc cataatttta tactattcta
    13381 tatatatgtc tcttcgatat tccaaatcat tattgtcagt cttgccgtta ttgatagttt
    13441 cttgaattat tcttgtataa cttatggggt gtccttttcg gcttatgtat atttcaactc
    13501 ttctgttttt agccctgcct tcagcagtac tgttgtcagc tatgggactg tataatccat
    13561 atcctattgc caggtattta gcagggtcta taacatcctt attttctaaa tatcttagaa
    13621 caacattggc tctgttcgta gataatgacc tgtcaatatc cgtcatatca tattcaacct
    13681 tagcagtgtg acctgctata acaacttcct ctatatattc gtcgaccatt gctaaccctt
    13741 ccgctaatac atcgagcact ccttttcctt tttgctctaa tatgtcgcta taccctctaa
    13801 aagttaccga atcggaaaat cttatgaaca catattcttc acttttgctt atttctactt
    13861 gccctgccaa ttcattttca tcaatatatt gtttaagaag cgtatacaat acatcaatat
    13921 cttctatttc ttccggttct tcttgttcct catatgaact gactgtttct tgctctgctg
    13981 cttcagcttc catcatctga taatatgctt cagggttcat ttctttagaa aacgaagcaa
    14041 ctattgcagc aaatttttgg gcatcaattg ttgacatagc gtaaagcatt ataaaaaagg
    14101 ttaacaataa ggttaccata tcgctgtatg tttccagcca gcttgctccg ccgctttctt
    14161 ctcttttctt tcttctcgcc ataatatcct actcatttct attattaagc attagcttct
    14221 ttttctttcc catttctttt tgaccttttt ttagattttc cttgtccttc ggctcctgcc
    14281 atggtgcttc tttcctgata cgttacgtag gaatttaatt tttctcttat atatttaggg
    14341 ttttctcccg attgtatgga taaaacacct tccattatca attccttagc aatcatttcc
    14401 tcgtcgtgct ttgcttttaa cctgttcgat atgggtgcac aaatcatatt tgtaagtacc
    14461 gagccgtaaa aggtagtaac taatgctact gacattcctt ttcctagcgc atctgctcct
    14521 tcgcctgcat ccatgtttgc aagcatattt ataagtccga ttaatgttcc aagcataccg
    14581 aatgcgggac caaatgagcc aagtgtatca aaaaccttcc aggcagaagc atgccttgct
    14641 tctatacaat ctatttcatt ttcaatttgt gcttttacct ttgtaggctc aatagcatct
    14701 actataagca tgacgcaata ctttaaaaac tcatcctgca agtccatttg atttaccttt
    14761 tcttcaagaa ccaaaag
//
LOCUS       DAOSHP010000040        14614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3388_length_14614_cov_9.287039, whole genome shotgun sequence.
ACCESSION   DAOSHP010000040 DAOSHP010000000
VERSION     DAOSHP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3388_length_14614_cov_9.287039"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..821
                     /locus_tag="PLB33_05405"
     CDS             285..821
                     /locus_tag="PLB33_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOG62994.1"
                     /translation="MNIGENLKRLRVANSLTQQELADRCELTKGYISQLERDLTSPSI
                     ATLTDILECLGTDLGSFFNEAVDSKIVFKQDDVFVKESEDNYIINWIVPNAQKYKMEP
                     ILVELGSGSKTKTDEPHEGEEFGYVLNGSILLNYGNQVYKVKKGESFYIKANKTHYIE
                     NVGKSKARVLWVSTPPYF"
     gene            834..1874
                     /locus_tag="PLB33_05410"
     CDS             834..1874
                     /locus_tag="PLB33_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG62995.1"
                     /translation="MNNAIIQLNNITKKFDGEIVLDDITLSVKENEFVTFLGPSGCGK
                     TTTLRIIGGFETPDEGEVYFDGKLINDVPPFKRQLNTVFQKYALFPHLNVFENIAFGL
                     RIKKMDEKTISEKVEKMLELFNLKNYGKRRIDQLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRKEMQHELKAMQKQLGITFIYVTHDQEEALTMSDTIIVMNEGIIQQMG
                     TPIDIYNEPENAFVADFIGESNILDGIMIDDYLVEFLGKRFECLDSGFGRGEEVDVVI
                     RPEDLDLTPAGEGKLEGIVKSVTFKGVHYEMDIVANNFEFLVHSTDMAPVGTTVGLTL
                     TPDDIHIMEKGE"
     gene            1877..2719
                     /locus_tag="PLB33_05415"
     CDS             1877..2719
                     /locus_tag="PLB33_05415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62996.1"
                     /translation="MKNRTKYFAYPYVLWIFLFIALPAFLVLLYSITTKESNGLTTIH
                     FTLENFKKFFQPMYLNILWDSIYLAAISTIICLILGYPAALIISRAHVSKRNTLLFLC
                     ILPMWMNMLLRTYAWMTILGNNGILNNIFAFLGLPRFNMLYTRGATVMGMVYNFIPFM
                     ILPIYTALSKMDKALLEAADDLGADKRTVFRKVIFPLSMPGVISGIIMVFMPAVSTFI
                     IPQLLGGNKSMMIGNLVEKLFILNGDWNFGSAISIVMMLIILLSMSIMNKFDVDKEGG
                     ARLW"
     gene            2713..3513
                     /locus_tag="PLB33_05420"
     CDS             2713..3513
                     /locus_tag="PLB33_05420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG62997.1"
                     /translation="MVKKYLSKIYMGIIFIFLYAPIIVLGVFSFNESKSRGNWTGFSL
                     KWYIELFHDHDIRMAFYYTITIAIITAIVSTILGTIAAIGINAMKGRQQALILNVNYL
                     PVVNPDIVTGISLMILYISFNMDFGFMTMLLSHIVFSTPYVILSILPKLKQLDVHMTE
                     AAMDLGATPMYALRKVILPEIKPGIITGFLMAFTLSIDDFIISYFTKGEGVTNLSIVI
                     YSMARRGVRPTINALSTIMLIVVLLLMILINKRTNGHDKPKKIYRSEV"
     gene            3515..4555
                     /locus_tag="PLB33_05425"
     CDS             3515..4555
                     /locus_tag="PLB33_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOG62998.1"
                     /translation="MKRKLILTFLVLSLIILAATGCSSEEKQTLSILNYDIYIDKSLL
                     TEFEKANDVTIKYDTYSTPEEMYIKVKSGASKYDLIISSEYMIERMINEKLVNKLNFD
                     NIPNYKYIGEDFKNHVYDPDNEYSVPYFWGTLGILYNKKTVDASSDSWAMLWDENHAQ
                     KIIMMDSQRDSFAAALKLLGYSINTVDEGELDEAKELLIKQRPLVMAYITDGSPAIMV
                     TEEADMALVWSGEAVAAMSENENLDFVIPKEGSNIWIDAMVIPSTTQNQELAEKFINF
                     LCSTEATLRNIDEVWYSTVHTEAIKQVDEELLNNPAFNIPKEDIAKMEMLRDPKEFIE
                     LYTERWTELMAQ"
     gene            complement(4621..5457)
                     /gene="ytxC"
                     /locus_tag="PLB33_05430"
     CDS             complement(4621..5457)
                     /gene="ytxC"
                     /locus_tag="PLB33_05430"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtxC"
                     /protein_id="HOG62999.1"
                     /translation="MELLSLELNENENHEIYNNIRIITNIEESDINIELSSTDEGNIK
                     IRYFTDNKINKREYLEKITAKITKLLVEYTKLVGIKILKENYFYFDDEEVSTIISDIE
                     EEIDNDIKIHIIIKNKFKEVIEKSNYINLNGFVNFRLKFLKLYAAQVVEKCIDSYLMK
                     KEYMDFISIIKLISDSEENQYDIVNVMFNNNKIQVYDKNMQKLNYFSNVELSTELNTE
                     STYDETVINILMSVSPKKIILHDLKIDKKNKEAYNTADIIKRIFEGKVETCSGCKYCE
                     FL"
     gene            complement(5485..6219)
                     /locus_tag="PLB33_05435"
     CDS             complement(5485..6219)
                     /locus_tag="PLB33_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63000.1"
                     /translation="MNPYIPTQKPRYVIIDYRASEEILNYLKKLNIEPIKSIKCNELH
                     EPVAGHPDMVIFPIDFKTFVAAPNVYDYYRNVLGSLGIKVIKGGKTLSRNYPEDIAYN
                     VARIGRYALHNTKYTDQVLKYYLEEAGIQFIHVNQGYTKCSIAPVSDNKALTSDFLIH
                     EKLISYNIDCMYIDPKVVYLKGYNNGFIGGCTGLINEKIFLSTGKIFDENISVSLREF
                     IQSSGYIYDEASKQQITDLGTLIPII"
     gene            complement(6426..6620)
                     /gene="thiS"
                     /locus_tag="PLB33_05440"
     CDS             complement(6426..6620)
                     /gene="thiS"
                     /locus_tag="PLB33_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOG63001.1"
                     /translation="MKLNNRDFPWEEGLTIEKIIEMKSFTFPKIIVKVNGKHIEKEDY
                     PTTIINDGDNVEIIHLLAGG"
     gene            complement(6664..8820)
                     /locus_tag="PLB33_05445"
     CDS             complement(6664..8820)
                     /locus_tag="PLB33_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG63002.1"
                     /translation="MDIIKMKENHKVLTEYKYELGEIDKGYTNRTLYVNISDNTITSK
                     PITEMMKEKFVGGRGFGLWYLWNATSPETKWDSPENEIIIAGGPVCGITQYAGTGKSL
                     VCSISPLTDIPIDSNVGGFFGPLLKFSGWDALEIQGKSDKDIIVYIDGNNGLVTIEEA
                     PLEAVDAHVLGEQLTEMYADNEDDKRNVAVVTAGTGAEHSRFGILNFTFYDPRRKVTR
                     LKQAGRGGIGTVFRNKKLKALIVKYSGVKGNLNNNFDQSIINKTGIKYHKEIHDLDHL
                     QNNMRKIGTANIIEIMDAYDLLPVMNFQYGSHPDTQNIKSTVFIEKYLTQGVQDGCWY
                     GCSMACAKGADNFLIKTGPYKGHRVTVDGPEYENAAGLGANCAIFDPEAILELNFYCD
                     TYGIDTISFGTATAFAMECYERGIINKEITGGLELNFGNFYASVELLHQMSKGEGFGT
                     VVGQGVHRMKKIFVEKYGADEEFLFDIGMEQKGLEYSEYGSKESLAQQGGYGLTNKGP
                     QHDEAWLIFMDMVNNQIPTFEDKAEALHYFPMFRTWFGLYGFCKLPWNDIVPADNKYT
                     DEPAKVPEHVQNYLDVVYGVTGKKLTVDDMIKESERVYNFQKIFNIRMGKGLRKNDKT
                     PYRSMGPVTKEEYESRQERYDKQLKEIIGYDIEGKTTEEKMAVLRKYRESQYEKLIDA
                     VYKRRGWNSNGVPTLEHLKYLDMELPELIEVIKDLQ"
     gene            complement(8830..9258)
                     /locus_tag="PLB33_05450"
     CDS             complement(8830..9258)
                     /locus_tag="PLB33_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG63003.1"
                     /translation="MKYLAKDDSKCIQCGACESICSKTYFKEDNPEKSCIQIKKEDTP
                     EINVCNQCGECIDICPVMAIKRDKFGVVRIDKKICVGCFMCVGFCPLLSMRQHDDYIE
                     PFKCIACGLCAKECPTEAIYIAERSDEEAREMPIDKVFNR"
     gene            complement(9407..10288)
                     /gene="hslO"
                     /locus_tag="PLB33_05455"
     CDS             complement(9407..10288)
                     /gene="hslO"
                     /locus_tag="PLB33_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOG63004.1"
                     /translation="MDYMIRAMDKKHTFRLFMVRATNTVEEARQHHNTTPTASAALGR
                     TLIAGLMMGYMMKNEKDRLTININGGGPIGTILVVSDNSGHVKGYVGNPNVDLDLKEN
                     GKLNVGGAVGTQGKLTVMMDIGLKEPYVGSTDLVTGEIGEDIAFYYYLSEQQNTAVAL
                     GVLVDIDYSIKAAGGFMVQTLPFIEEEDLVKLENTLNNLKSISDYFNNEKDLEEIAKE
                     IFSDFDIEVFDRIPVSFNCDCSEERMEQALISLGKNELKKIIEEDGKIETVCHFCNKK
                     YMFEGDKLKNILKYIEE"
     gene            complement(10291..11025)
                     /locus_tag="PLB33_05460"
     CDS             complement(10291..11025)
                     /locus_tag="PLB33_05460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG63005.1"
                     /translation="MKNSYKEFSQIYDLLMDIDYEKWTSFIMKKLKGKRKILEAACGT
                     GGITKLLADNNYKVTAFDLSEDMLMRAYEKLAQSPMVRLLNQNMVDFEIDEKFEAAIC
                     CCDGINYLIKEEVKSFFQRIYHHLNEGSKFIFDMSTKYKYDKMFCDTYVYDDGDIFYV
                     WENELVNDEKINIEINFFIKDTDKYTRITEEQTQYIHTVDEIIDLLEDAGFENIEIFD
                     DYNEKEYSDTSLRAVFSACKFDNERT"
     gene            complement(11037..11237)
                     /locus_tag="PLB33_05465"
     CDS             complement(11037..11237)
                     /locus_tag="PLB33_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOG63006.1"
                     /translation="MAEKKEKNKKNKKKKEPTPNDIMKMEIAEELGLTDKVKKFGWGG
                     LTAKETGRIGGLMTAKKKKRKK"
     gene            complement(11294..11431)
                     /locus_tag="PLB33_05470"
     CDS             complement(11294..11431)
                     /locus_tag="PLB33_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63007.1"
                     /translation="MLDFDFRVIAVVMFFIILISIQFTLNKIFLLLREIRAILLTKRD
                     R"
     gene            complement(11539..11991)
                     /locus_tag="PLB33_05475"
     CDS             complement(11539..11991)
                     /locus_tag="PLB33_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOG63008.1"
                     /translation="MYDIKKILVPVDGSPASIKASERAIEIAKKYGSEITFLSVANTP
                     DMFNIGDYSFRVHYNYDNVVNNHKLAQAEILDKVIEGLDIEGVKFEKKVISGEPAEEI
                     IRMAHDCQYDYIVMGRRGFSKFTRFFVGSVTQRVIAESPCAAVIVVNE"
     gene            12205..12918
                     /locus_tag="PLB33_05480"
     CDS             12205..12918
                     /locus_tag="PLB33_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOG63009.1"
                     /translation="METKKETIIKAAGAAFPQTIPILAGYVFLSIAYGIYMNAFGFNF
                     LYPMFMSMLIYAGTVQFVAVGLLMAVFDPINAFVLTLMINARYLFYGISMLEKYRVKG
                     IRGAFLIFSMSDETFSINCTANIPEDVDKGWFMFFVTLFNYCYWVSGSILGGLFGELI
                     EFNTKGLEFAMTAMFIVIFMEQWLKEKSHSSSLLGLGISLLCLIIFGSKNFIIPSMIL
                     ILLILSLKRELFMEAGESK"
     gene            12915..13247
                     /locus_tag="PLB33_05485"
     CDS             12915..13247
                     /locus_tag="PLB33_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HOG63010.1"
                     /translation="MTMTVGQHIITIAMVVLGTMITRFLPFIIFPADKPTPKYIQYLG
                     KALPPASLGLLVVYSLKDVNIFTGSRGIPELIAVITVLVIHLWKRQMLLSIAVGTITY
                     MLLVQFVL"
     gene            complement(13306..13387)
                     /locus_tag="PLB33_05490"
     tRNA            complement(13306..13387)
                     /locus_tag="PLB33_05490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13351..13353),aa:Leu,seq:cag)
     gene            complement(13472..14215)
                     /locus_tag="PLB33_05495"
     CDS             complement(13472..14215)
                     /locus_tag="PLB33_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOG63011.1"
                     /translation="MENKVLAIVDGREIKESDLKNLLRNLGQNAVHFQGEEGRKKLID
                     ELVMHELMYSDAVENNLENEEEFIEVFNDMKKSMLQQYNLRKMFNKVTISDEELKDFY
                     EKNKYFYNRPEMVKASHILVDTEERANEILEDITDGLSFEDAAKEYSNCPSKQDGGFL
                     GQFGKGQMVKEFEDAVFSMRAGEISPPVKTQFGYHIIKLIEHMPARSAEFEEVYQEVK
                     DNYFVIKQEEVYKSKKEELIKKYDVVLFE"
     gene            complement(14265..14531)
                     /locus_tag="PLB33_05500"
     CDS             complement(14265..14531)
                     /locus_tag="PLB33_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOG63012.1"
                     /translation="MRSQKVVLKNKIGLHARPAALFVKKARNFDSEIIIKKGIQEANG
                     KSIISIMALGAMKGDELVIITKGSDEDKCIAELTNLLEFMEEEE"
BASE COUNT         4337 a         2719 c         2325 g         5233 t
ORIGIN      
        1 taacgcaaac ctgtataata tgtcaatagt agtataaata acatccttgc ctgtccttgg
       61 tatgaaaaac cgtcatttag agggcgtagc cctcgttatt ccgagatcct ttttcttacg
      121 atcaggatga caaggcttcg ttcagactca gaattataac tccgtttaag caaaaaataa
      181 tgaaaaatta ttattgacag aaggggtgtg gatatgtata ataaaagctg tttcagtgtt
      241 tagtaatttt aaactttgag tttagtaaaa aatgtgaggt ttatatgaat attggagaaa
      301 atttaaaaag gttgagagtt gccaattctc tcacacagca ggaattggct gacagatgcg
      361 aattgacaaa ggggtatatt tcgcagcttg agagagattt gacttctcct tccatagcaa
      421 ctcttacgga tatattggag tgtctgggga cggatttagg aagctttttc aatgaagccg
      481 tggacagtaa aattgtgttt aaacaagatg atgtatttgt aaaagaatca gaggacaatt
      541 atattattaa ctggattgtt ccaaatgcac agaaatataa aatggagccc atattggtgg
      601 aactcggaag cggcagcaaa acaaaaacag atgagcctca tgaaggcgaa gagttcggtt
      661 atgttttaaa cggctccata cttttaaact acggcaatca agtgtacaag gttaaaaagg
      721 gagagtcttt ttacattaag gcaaacaaaa ctcattatat tgaaaatgtg ggaaaaagca
      781 aagcgcgtgt actatgggta agtactccgc cgtattttta gcaggaggta agaatgaata
      841 atgcaattat acaattaaat aatattacca aaaaatttga cggagaaatt gttcttgacg
      901 atattacctt atccgtgaag gaaaatgaat tcgtaacttt tttgggacca agcggctgtg
      961 gaaaaacaac tactcttaga attatcggag gctttgaaac tcctgatgaa ggtgaagttt
     1021 attttgacgg caaattaatc aatgacgttc ctccttttaa aagacagctg aataccgtgt
     1081 ttcagaaata tgctttgttt cctcatttaa atgtttttga aaacatagca tttggtctga
     1141 gaataaagaa aatggatgaa aagaccattt ccgaaaaggt tgaaaaaatg cttgagcttt
     1201 ttaatctcaa aaattacggc aaaagaagga ttgaccagtt aagcggagga cagcagcagc
     1261 gtattgcaat agcaagagct ttggtaaatg agcccaaggt acttttgctc gatgaaccct
     1321 taggtgcttt ggatttaaaa ttaagaaaag aaatgcagca tgagctcaaa gcaatgcaga
     1381 aacagcttgg aataacattt atttatgtaa ctcatgacca ggaagaagct ttaacaatgt
     1441 cagacacgat tatagtcatg aatgaaggca ttattcaaca aatgggtact cccatagata
     1501 tatataatga gcctgaaaat gcgtttgtgg cagattttat cggagaaagc aatatattgg
     1561 acggaattat gatagatgat tacttggttg aatttttagg aaagagattc gaatgtctcg
     1621 attcaggatt tggaagaggc gaagaagttg atgtggttat tcgccctgaa gatttagatt
     1681 taactcctgc cggtgaagga aagctggagg gaattgtaaa gtcggtaaca tttaaaggtg
     1741 ttcattatga aatggacata gtagcaaata attttgagtt tttggttcat tcaaccgata
     1801 tggcccctgt aggaacaact gtcggtttaa ccttaactcc tgacgatatt catataatgg
     1861 aaaaggggga atagctttga aaaacagaac aaaatatttt gcttacccct atgtcttgtg
     1921 gatatttttg ttcattgctt taccggcatt tttggtgctt ttatacagca ttaccacaaa
     1981 agaatctaac ggacttacaa cgattcattt tacattagaa aatttcaaga aattttttca
     2041 acccatgtat ttgaacattt tatgggactc catctactta gcggcaattt caaccataat
     2101 atgtttgata ctcggatacc ctgcggcatt aataatttct agggcacatg tgtcaaagag
     2161 aaatactttg ttgtttcttt gtattttgcc catgtggatg aatatgctcc ttagaactta
     2221 tgcttggatg accatacttg gaaacaatgg aattttaaat aacatttttg cttttttagg
     2281 tcttccgagg tttaacatgc tctatacaag gggcgctacc gtaatgggta tggtttacaa
     2341 ctttattccc tttatgatac tgcccattta tactgcatta agtaaaatgg ataaagccct
     2401 tttggaagca gccgacgatt tgggagcaga caaaagaaca gtttttagaa aagttatttt
     2461 ccccttaagc atgcccggtg taatttcggg catcataatg gtttttatgc ctgcggtaag
     2521 cacatttatc attcctcaac tcttaggagg aaataaaagc atgatgatag gaaacttggt
     2581 ggaaaaattg tttattttaa acggagactg gaatttcggc tcagccatat ccattgtaat
     2641 gatgcttata attcttttat ccatgtcaat tatgaataaa tttgatgttg ataaggaagg
     2701 aggagcaagg ttatggtaaa aaaatattta tcaaaaatat atatgggcat tatatttata
     2761 ttcctctatg ctccgattat agttcttggt gtattttcct ttaatgaatc aaaatcgaga
     2821 ggaaactgga caggattttc actgaagtgg tacattgagc ttttccatga ccacgatata
     2881 cgaatggctt tttattacac aataacgata gcaatcatta cggcaatagt atctacaatt
     2941 ttgggtacaa tagcagcaat aggaataaat gcaatgaagg gcagacaaca agctttaata
     3001 ctcaatgtta attaccttcc agttgtaaat cccgatattg ttacgggtat atccctgatg
     3061 attttatata tttcatttaa tatggatttt ggatttatga caatgctatt gtctcatata
     3121 gtgtttagca ctccctatgt aatattgtca atacttccaa agttaaagca gctcgatgta
     3181 cacatgaccg aagctgcaat ggatttaggg gcaactccca tgtatgctct aagaaaggta
     3241 atacttcctg aaataaagcc gggaattatt acgggatttt tgatggcatt tacattgtcc
     3301 attgatgatt tcatcataag ttactttacc aagggcgaag gtgttacaaa cctttcaatt
     3361 gtgatttatt caatggcaag aagaggtgtc cgccctacaa tcaacgctct ttcaacaata
     3421 atgctgatag tagtattatt gttaatgata ttgataaaca aaagaactaa cgggcatgat
     3481 aaacccaaaa aaatatatcg aagtgaggta tagaatgaaa agaaaattaa ttttgacatt
     3541 tttagtttta agcttgataa ttcttgctgc aaccggatgc agctctgaag aaaaacaaac
     3601 attgagtatt ttaaactatg atatttacat tgacaaaagt ttattgactg aatttgaaaa
     3661 agccaacgat gttacaatta aatatgacac gtattcaacg ccggaagaaa tgtatataaa
     3721 ggtgaaatcc ggggcttcaa aatatgattt gattatttca agcgaatata tgattgagcg
     3781 catgattaat gaaaaattgg ttaataaatt aaatttcgat aacattccga attataaata
     3841 tatcggcgaa gattttaaaa accatgtata tgaccctgat aacgaatatt cggttccgta
     3901 tttctggggg accttaggca tattgtacaa taaaaaaact gtagatgcat cttctgacag
     3961 ctgggctatg ctttgggatg aaaatcatgc tcaaaaaatt ataatgatgg attcgcaaag
     4021 agattcattt gcagctgcgc taaaattatt gggatattca attaacacgg ttgatgaagg
     4081 agagctcgat gaagcaaagg aactccttat taagcaaaga cctcttgtta tggcttatat
     4141 aacggacggt tcacctgcaa ttatggtaac cgaagaagct gatatggcgc ttgtttggtc
     4201 aggtgaagcg gttgccgcaa tgtccgaaaa tgagaatttg gattttgtaa ttccaaagga
     4261 aggcagcaat atttggattg atgctatggt tattccttcg actacccaaa atcaggaatt
     4321 agccgaaaaa tttattaact tcttatgctc caccgaagca acgttaagaa acatagacga
     4381 agtatggtat tctacagtac ataccgaggc tattaaacaa gtggatgaag aactcttaaa
     4441 caaccctgct tttaacattc caaaggaaga tatcgcaaag atggaaatgt tgagagaccc
     4501 gaaagaattt atagaattat acactgaaag atggactgaa ttaatggcac agtaatcatc
     4561 tttaaattaa aaccggtgcc gggcacccaa tagggtgcct ggcaccgctt catatatatt
     4621 ttataaaaac tcacaatact tacatccgct gcaagtttcg actttacctt caaatattct
     4681 ttttattata tcagcggtat tatatgcttc tttatttttc ttatctatct ttaaatcatg
     4741 caatattatt ttttttggtg acacactcat taaaatattt attacagttt cgtcgtatgt
     4801 tgattcggta ttcaattcag ttgaaagctc tacatttgaa aaataattga gcttttgcat
     4861 attcttgtca tatacctgga ttttattgtt attaaacatt acatttacaa tatcatattg
     4921 attttcttcc gaatctgata tcagttttat aatgcttata aaatccatgt attccttttt
     4981 catcaaatat gagtcaatgc acttttctac tacctgtgct gcataaagct ttagaaattt
     5041 tagtctgaag tttacaaatc cgtttaaatt tatataattg gacttttcta taacttcttt
     5101 aaatttattt ttaattatta tgtgtatttt aatatcattg tctatttcct cttcgatatc
     5161 ggatattatt gtgcttactt cttcatcgtc aaagtaaaaa taattttcct ttaaaatttt
     5221 tataccaact aacttagtgt attcaaccaa aagcttggtt atttttgctg ttattttttc
     5281 aaggtactct ctcttgttta ttttattatc cgtaaaatat cttattttta tatttccttc
     5341 atcggtagac gataattcta tatttatatc actttcctct atgttagtta taatccttat
     5401 attattataa atttcatgat tttcattttc atttaactct aatgatagta gctccactta
     5461 gtcactcctc tcggtcaagg gaggttatat tatcggtatc agcgttccta agtctgtaat
     5521 ttgctgtttt gaagcttcat cataaatata tcctgacgac tggatgaatt ctcttaatga
     5581 aacagaaata ttttcatcaa aaatttttcc ggttgacaaa aatatttttt cattaatcaa
     5641 tcctgtacag cctcctataa atccgttgtt gtatcctttt aaatatacaa cttttgggtc
     5701 tatatacatg cagtctatat tgtatgatat tagtttttca tgtatcaaga aatccgatgt
     5761 caaagcttta ttatcactga caggagcaat tgagcacttt gtatagcctt gatttacatg
     5821 aatgaattga atgcccgctt cttccaaata atattttaaa acctggtctg tatactttgt
     5881 attatgcaaa gcatatctcc ctattcttgc tacattatat gctatatcct caggatagtt
     5941 tctgctaaga gtttttccac ctttaataac ttttattcct aggcttccca aaacatttcg
     6001 gtaataatca taaacgtttg gagctgcaac aaaagtttta aaatctatgg gaaaaattac
     6061 catatccgga tgaccggcca caggctcatg cagctcattg catttaatgg attttatagg
     6121 ctctatattt aattttttta gataattcag tatttcttca cttgctctgt aatcaataat
     6181 tacatagcga ggcttttgag tcggaatgta tggattcata ttatctcccg ttgaatgagt
     6241 ctcattcaca gattatatca ttaaggtata tattattcta caaaaaatca tgaggttctt
     6301 caagctagct ctcagaacga caaaaatgct atttatagta aaataaaatg atataattat
     6361 aaaattatat catttttata ttaatccaaa taaatttatc aggcaatttc tacaagtgag
     6421 ttgatttatc ctcctgccaa gagatgtata atttcaacat tatcaccgtc attaataata
     6481 gttgttgggt aatcttcctt ttcgatatgt tttccgttta ctttaactat tattttagga
     6541 aaggtgaaac ttttcatctc tataattttt tctattgtca atccctcttc ccagggaaag
     6601 tctctattgt ttagttttat aatttatacc acctccgaat gtaactatac tcccgtcccc
     6661 ttgttattgc aggtctttga ttacttctat taattcagga agttccatat ccaaatattt
     6721 tagatgttct aatgtcggga cgccgtttga attccatcct cttcttttat agactgcatc
     6781 tataagcttt tcatattggc tttctctata ctttctcagt accgccatct tttcttcggt
     6841 agtcttgcct tctatatcat aaccgattat ttctttaagc tgtttgtcgt atctttcctg
     6901 ccttgattca tattcttcct tggttaccgg acccattgag cggtaaggag ttttatcgtt
     6961 tttccttaaa cccttaccca ttcttatgtt aaaaattttc tggaagttat aaaccctttc
     7021 agattctttt atcatgtcat caacggttaa tttcttgcct gtaactccat ataccacatc
     7081 aagataattt tgaacatgtt caggcacttt tgcaggctca tcggtatatt tattgtccgc
     7141 cggaactatg tcattccaag ggagcttgca gaatccatac aagccgaacc aagtcctgaa
     7201 cataggaaag taatgaagag cttcagcctt atcttcgaag gttggtattt gattgtttac
     7261 catatccatg aaaatcagcc atgcttcatc atgctgagga cctttgtttg ttaaaccgta
     7321 tcctccctgc tgagccagag attctttcga gccgtattcc gaatattcaa gccccttttg
     7381 ctccattcct atgtcaaaca agaattcttc atcagctccg tatttttcaa cgaatatttt
     7441 tttcatcctg tggactccct gacctacaac tgttccaaag ccttctccct tggacatctg
     7501 gtgcaagagt tcgactgatg cataaaagtt gccgaaattc aattctaagc cgcctgtaat
     7561 ttccttgttg attatacctc tttcatagca ttccattgca aatgccgttg cagtaccaaa
     7621 tgaaattgta tctatgccat aggtgtcgca atagaagttt aattccaaaa ttgcttccgg
     7681 gtcaaaaata gcacaatttg ctcctaatcc tgccgcattt tcatattcgg gaccgtctac
     7741 tgtaactcta tgtcccttat aaggtcctgt ctttatgagg aaattatccg cacctttggc
     7801 gcaggccata gagcagccgt accagcagcc gtcttgaact ccctgagtca ggtatttttc
     7861 aataaatacc gtggatttaa tattttgtgt gtccggatgg cttccatatt ggaaattcat
     7921 aacaggaagc aaatcatagg catccattat ttcaataata tttgccgtgc ctatttttcg
     7981 catattgttt tgcaaatggt ctaagtcatg aatttcctta tgatatttta ttccggtttt
     8041 attaattatt gactggtcaa aattattgtt taaattgcct tttacaccgc tgtattttac
     8101 aatcagtgcc ttaagctttt tatttcgaaa tacggttcct attccacccc tgcctgcttg
     8161 ttttagtctt gttacttttc ttctggggtc atagaatgta aaatttaata ttccaaaacg
     8221 ggaatgctcg gcacctgttc ctgcggttac aacagctaca tttcttttat catcttcatt
     8281 gtccgcatac atttcggtaa gctgctctcc caaaacatgt gcatcaacag cttccaatgg
     8341 tgcttcttca attgttacaa ggccattatt cccatcaata tatacaatta tatctttatc
     8401 tgatttgccc tgtatttcaa gagcgtccca tcccgaaaat ttcaaaagag gtccaaaaaa
     8461 tccgcctaca tttgaatcta tgggaatatc cgtcaagggt gaaatgctgc ataccaatga
     8521 ttttcctgtt cctgcatatt gagttatacc gcagacagga ccccctgcaa taattatttc
     8581 attttccggg ctatcccact tcgtttcagg acttgttgca ttccacagat accacaagcc
     8641 aaaacccctt ccccctacaa atttttcttt catcatttcg gttatcggct tagaggttat
     8701 tgtattgtct gaaatattaa catacagtgt tctgtttgta tatcctttgt ctatttctcc
     8761 gagttcatat ttatattcgg ttaagacttt atgattttct ttcattttta taatatccat
     8821 tttgtcctcc tatctgttaa aaaccttatc tattggcatt tctcttgctt cctcatcgct
     8881 tctttcagct atataaattg cctccgtagg acattctttg gcacataatc cgcatgctat
     8941 acatttaaaa ggctctatat aatcatcatg ctgcctcatg gataagagag ggcaaaatcc
     9001 cacacacata aaacaaccga cgcaaatttt cttgtcaatt ctaacaaccc caaacttgtc
     9061 cctctttatt gccataacag gacaaatatc gatgcattct ccgcattgat tgcaaacatt
     9121 tatttcaggg gtgtcctctt ttttgatttg tatgcatgac ttttccggat tatcctcttt
     9181 aaaatatgtt ttagagcata tgctttcgca agccccgcac tggatacatt tactgtcgtc
     9241 tttagccaaa tatttcatac tgacctcctc ttgtatgtta atattaatta gatttcattc
     9301 atttataatc acttatatta tataaatatt tttaatatat ttcaatttct tatagtatta
     9361 ttaatgtgct aatatttttc tattagttgc tccttttctc gcaatatcat tcttcaatat
     9421 atttcaaaat attttttagt ttgtctcctt caaacatata tttcttattg cagaaatggc
     9481 atactgtttc tattttcccg tcttcctcaa ttattttttt cagctcattc ttacccaatg
     9541 aaattaatgc ctgctccatt ctttcctcgg agcagtcaca attaaaagaa acgggaattc
     9601 tgtcgaaaac ctcgatatca aaatcggaaa atatttcttt tgcaatttct tctagatctt
     9661 tttcgttatt aaaataatct gatattgatt ttagattatt gagggtattt tctaatttaa
     9721 caaggtcttc ttcctcaata aaaggcagtg tttgaaccat aaagcctcct gcggctttaa
     9781 tgctgtaatc tatatccact aatacaccca aggctacggc agtattttgc tgttccgaca
     9841 aataataata aaatgctatg tcttctccaa tttctcctgt taccaaatcg gtgcttccca
     9901 catagggctc cttcaatcct atatccatca taacggtgag cttgccttga gtcccgacag
     9961 ctcctcctac gtttaattta ccgttttctt tcaagtctaa atcaacattc ggattgccaa
    10021 catacccctt aacatggcct gaattatccg aaacaaccaa aattgtacct atgggacctc
    10081 caccgtttat attaattgtt aatctgtctt tttcgttttt catcatatag cccatcatga
    10141 gaccggctat aagagtcctg cctaaagcag cagaagctgt cggtgtcgta ttatggtgct
    10201 gccttgcttc ttccaccgta tttgttgccc ttaccataaa aagtctaaaa gtatgttttt
    10261 tatccattgc tctaatcatg taatccattg ttatgtcctt tcattgtcaa atttgcatgc
    10321 ggaaaaaact gcccgcaaag aggtgtcaga atattctttt tcattgtagt catcaaatat
    10381 ctcgatattt tcaaatcctg catcctcaag taagtctatt atctcatcaa ctgtatgaat
    10441 atattgagtt tgttcttcag taattcttgt atatttatct gtgtccttaa taaaaaagtt
    10501 tatttcaata tttatttttt catcattaac aagttcattt tcccaaacat aaaaaatatc
    10561 tccgtcatca taaacataag tatcacaaaa cattttatca tatttgtatt ttgtgctcat
    10621 gtcaaaaatg aatttggagc cttcatttaa atgatggtaa atcctttgga aaaaagattt
    10681 cacttcttcc tttatgaggt aatttatgcc gtcgcagcag caaattgctg cttcaaattt
    10741 ttcatctatt tcaaaatcaa ccatattttg attcaataat cgaaccatag ggctttgcgc
    10801 cagcttctca taagccctca taagcatgtc ctcagacaaa tcaaaggcag ttaccttgta
    10861 attgttatcg gctaaaagct ttgttatgcc tcccgtgccg caggcagctt ccaagatttt
    10921 tctttttccc tttaatttct tcattataaa ggatgtccac ttttcgtaat cgatatccat
    10981 aagcaaatca taaatttgag aaaattcttt atagctgttt ttcatttact tttcctttac
    11041 tttttccttt tctttttctt agctgtcatt aatcctccga ttctgcctgt ttccttggca
    11101 gttaatcctc cccagccgaa ctttttaact ttatccgtaa gtccaagctc ttctgctatt
    11161 tccatcttca ttatatcgtt tggtgtcggt tcttttttct tcttgttttt cttgttcttc
    11221 tctttctttt cagccataat accctccttg taatattatg gcttttaaaa ggaaacttat
    11281 tctaataatc tgtttatcta tctctcttag tgagtaaaat tgcccttatt tctcttaaaa
    11341 gcaaaaatat tttattcaga gtaaattgta tacttattaa aattataaaa aacattacaa
    11401 cggctattac tctaaaatca aaatctaaca tattttctcc ttgaaatgag accatctttt
    11461 ttcaataata tcacaagaaa aaaaccattt caagaaaaaa ccccctgggt cagggggaaa
    11521 tatatatagt tttgtatatt attcattaac aacaataacc gcagcacatg gggattcagc
    11581 gataaccctt tgagttaccg agcccacaaa aaatcttgtg aattttgaaa atcctcttct
    11641 tcccattaca atataatcat attgacaatc gtgtgccatt cttattattt cttcagcagg
    11701 ttctccggaa ataacctttt tttcaaattt aaccccttct atatccagac cttctataac
    11761 tttatccagt atttcagctt gagctaattt gtgattattt accacattat catagttata
    11821 gtgtactctg aaactataat caccaatgtt aaacatatcg ggtgtatttg ccaccgataa
    11881 aaatgtaatc tcactcccat acttctttgc aatttctata gctctttcag atgcctttat
    11941 cgaagccggc gagccgtcaa ccggtaccaa tattttcttt atatcataca taattaattt
    12001 atctcccttc attaatattg tttgatatat atatacctgt ttaaattcca attaaacatt
    12061 ttttaacaaa atttattgtt tataattctt ttgattgatt atgtgcagtt tcagtgatag
    12121 aatatagcga agtaaaaatt tctcctcttt gggtaagggg tatggattaa attacataaa
    12181 tttgattagg agtcatagaa atagatggaa acaaaaaaag aaaccataat aaaagctgcc
    12241 ggagcagctt ttcctcaaac cattcctatt ttggcaggct atgtattctt aagcatagcc
    12301 tatggtattt atatgaatgc tttcggattc aattttttat atcccatgtt tatgagcatg
    12361 ttaatctatg caggtacagt tcaatttgtt gcagtaggtc ttctgatggc agtcttcgac
    12421 cctataaatg cttttgttct gacacttatg attaatgcac gctatttgtt ttacggcatt
    12481 tctatgttgg aaaaatatcg ggttaaggga ataagaggag cctttttgat ttttagtatg
    12541 agtgatgaaa cattttcaat caattgcacg gcaaatatcc cggaagatgt cgataagggt
    12601 tggttcatgt tctttgtaac tctttttaat tactgctatt gggtatcagg ctccatactt
    12661 ggcggattgt tcggtgaatt gattgaattt aatacaaagg ggcttgaatt tgcaatgaca
    12721 gcaatgttca ttgtaatctt tatggagcag tggctgaaag aaaaaagtca tagcagctcg
    12781 ctgttaggat tggggatatc tttgttatgc ctaattatct ttggcagcaa aaactttata
    12841 ataccttcaa tgattttaat tttattaatc ctttctctaa aaagagaact ttttatggaa
    12901 gcaggtgaat caaaatgacg atgactgtcg gacaacatat tataacaatt gccatggtag
    12961 tacttggtac aatgattacc cgttttcttc cctttataat ttttcctgcc gataagccaa
    13021 caccaaaata tatacagtat ttgggaaagg cgctcccgcc ggcttccctg ggactgcttg
    13081 tagtgtacag tcttaaggat gtaaatatat ttacgggcag tcgcggtatt ccggagctga
    13141 ttgccgttat tacagtactt gttatccacc tgtggaaaag acagatgctt ttatccattg
    13201 ctgttggaac aataacttat atgctcttgg ttcaatttgt actatgactt tctaccatta
    13261 aaaaacggct ccctgtcgga agccgttaaa aagttgaatt attagtgcgg ataacaggat
    13321 ttgaacctgc acaggttgcc ccacacggac ctgaaccgtg cgcgtctgcc aattccgcca
    13381 tatccgctgg tcactgaaat atgttatcac aaatatataa tctttgcaat atttttttcg
    13441 tgctgtctca actttctcaa taagttatca attattcaaa taatacaaca tcatactttt
    13501 taattaattc ttctttttta cttttataaa cttcctcttg tttaattaca aaataattgt
    13561 cttttacttc ctggtaaact tcttcaaatt ctgcacttct agcaggcata tgctcaatta
    13621 atttaattat gtgatatcca aactgtgttt ttacaggtgg acttatttca cctgcgcgca
    13681 ttgaaaatac agcatcttca aattctttta ccatttgccc ttttccgaac tgtcccaaaa
    13741 atcctccgtc ttgcttggaa ggacagtttg aatactcttt agcggcatcc tcaaaagaca
    13801 aaccatctgt tatatcttcc aatatttcat ttgctctttc ctctgtatca accaagatgt
    13861 ggcttgcttt taccatttcg ggtctattgt aaaaatactt gtttttttca taaaagtctt
    13921 tcaattcttc gtcggatatt gtaactttgt tgaacatttt tcttaagttg tattgttgca
    13981 gcatcgactt tttcatatcg ttgaatactt cgataaattc ttcttcattt tccagattgt
    14041 tttctaccgc atccgaatac ataagttcat gcataaccaa ttcatcaata agcttttttc
    14101 ttccttcttc tccttgaaaa tgcactgcat tttgtcctaa attccgtaat aaatttttta
    14161 agtctgattc ttttatttct cttccgtcta ctatagctaa tactttattc tccatttttt
    14221 ctccttcgtc ataaaataag agggtctttt ccgcgccctc tttattattc ttcttcttcc
    14281 ataaattcca ataaattagt caactcagca atacatttgt cctcgtctga acctttggtg
    14341 attattacta attcatcgcc cttcatggct cccaaagcca ttatgctgat aatactcttg
    14401 ccgtttgctt cctgtatacc ttttttgatt ataatttcac tgtcaaaatt tcttgccttt
    14461 ttaacaaata aagccgcagg tctggcatgc agtccaatct tattttttaa cactactttt
    14521 tgtgatctca ctttattaca cctctctttg tatgctcgat taaatcttca gcaatttctt
    14581 ctatttttct tagtctgtga tttactcctg attt
//
LOCUS       DAOSHP010000041        14532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3416_length_14532_cov_8.048076, whole genome shotgun sequence.
ACCESSION   DAOSHP010000041 DAOSHP010000000
VERSION     DAOSHP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14532
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3416_length_14532_cov_8.048076"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLB33_05505"
     CDS             <1..819
                     /locus_tag="PLB33_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOG63013.1"
                     /translation="LQDVTSKRSIYYYLFLIRLAKLMNNKVVMLSQGIGPIINEKSIK
                     AVKNTLSLVDYITVRDKHSKEFLESIGVDERKIFLSTDPVINIRKDDSLEYEKQSVRK
                     VCFSLRNWKDSDVSVKISKVAQRLIDDGIECHFIPFYYKEDLQLIAQVEELLGSKAVY
                     YKERLSTGDAFDIIKTMDVMVGVRLHSLIFAAAANVPIVAVSYDQKVDNFVNSVNMKI
                     ACTIDNIDENKIYDEIIDKINNEREEKIMLLKSVEKLRELTNINYKILKEITNG"
     gene            812..1579
                     /locus_tag="PLB33_05510"
     CDS             812..1579
                     /locus_tag="PLB33_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOG63014.1"
                     /translation="MDKLSIMGVRINNISMNEVLKLAEQKIKNDEKYIIYTPNTEIIM
                     MCQKDEEFLNIMNKSNINVPDGVGLIYAGKIKKFPLKEKVAGYDLSINLLKMADEKGL
                     KLYAVGGRPGVAEAAMQNVQKKYPGIKIVGAHHGYFKGTHLGKFGHEEEIAVIEDINR
                     HKPHILFVGFGAKKQEQWIEYNKDLINANIIIGNGGTLDGLAGIVKRAPDIFINLGLE
                     WLYRLIKEPKRITRQIVLPVFMFKVIFGNKDIVKKIE"
     gene            1783..2427
                     /gene="fsa"
                     /locus_tag="PLB33_05515"
     CDS             1783..2427
                     /gene="fsa"
                     /locus_tag="PLB33_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOG63015.1"
                     /translation="MKLFIDTANVEEIRKANDMGVICGVTTNPSLIAKEGRIFKDVVK
                     EITDIVDGPISAEVISLDSEGMIKEAVELSKIHKNIVIKIPMTAEGLKAVKVLSKEKI
                     KTNVTLIFSAGQALLAAKAGASYVSPFVGRLDDIGNDGMNIIKETVEIFKNYNIDTEI
                     IAASIRHPMHVIEAAKLGCHIATIPYKVIIQLTKHPLTDKGIDQFLKDWDTVPK"
     gene            2442..3284
                     /locus_tag="PLB33_05520"
     CDS             2442..3284
                     /locus_tag="PLB33_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOG63016.1"
                     /translation="MKYDELKVIANSMRKNILTMIYKAKSGHPGGSLSAVEIMVYLYY
                     KEMKIDSPTDENRDRFVLSKGHGAPVLYAVLMEKGFISKDLIPTLRQIDSKLQGHPDM
                     KKLPGVEASTGSLGQGLSIANGMALAFKLDKKPNRVFALLGDGEINEGMIWEAAMLAS
                     HYNLDNLTAVLDHNGLQIDGKNEDVMTIEPIDKKFEAFGWHVIKTEGHSFESLEKAFE
                     ERKTIKGKPAIIIANTIKGKGCSFMENQAGWHGKAPNEGEFCKAMEEMGDIGDKTEGG
                     GYNG"
     gene            3277..4206
                     /locus_tag="PLB33_05525"
     CDS             3277..4206
                     /locus_tag="PLB33_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOG63017.1"
                     /translation="MAKATREAYGEALKKLAASNPDIVVLDADLSGSTKTSEFKKVSP
                     ERFFNVGIAEQNLIGTAAGLSLAGKIPFASSFAMFAAGRAFEIIRNTVAYPALNVKIA
                     ATHAGLTVGEDGGSHQAIEDISLMRTIPNMVVINPADSREAEQAVLKAAEYVGPVYIR
                     LGRMAVEDVYTDSYVFEWGKGVLLRDGKDVSIIATGLMVQEALKAHEILKEDGINAKV
                     INIHTIKPIDRDLIIRAAKETKAIVTAEEHSVIGGLGSAVLEVLSDEYPVPLKRVGIL
                     DTFGESGKPKDLLQKYRLTADEIVRQVKEVIKF"
     gene            4395..4886
                     /locus_tag="PLB33_05530"
     CDS             4395..4886
                     /locus_tag="PLB33_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOG63018.1"
                     /translation="MFNLGDKVVYPMHGAGVIETMESKEILGEIKNYYVLKMPIGEMK
                     LMIPVDNVNNIGLRNIIDKYAVEEVYKVLKQKAEINDSNWNKRYRDNMTKMKTGDIFE
                     VAQVVRDLTYRDREKGLSTGEKKMLVSAKQMLISEITLATGMDSEEIQDYMDEIINFD
                     ALE"
     gene            5089..6186
                     /locus_tag="PLB33_05535"
     CDS             5089..6186
                     /locus_tag="PLB33_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HOG63019.1"
                     /translation="MIDKIVKAVIAVIGASLGAVSGVLLNNSGLLELPSLQSTLVIIG
                     ISLVFGIIFFLLSSKIKKTGLKIIEALETEILKFSTMDIVLGSLGLIVGFIIAFLISQ
                     PLSDIKYVGTIISVISFVFFGYLGVKIATRKKDDVLWPQILNLKKSSTVKKQEKNKKE
                     IISPKILDTSVIIDGRISDICKTGFIEGTLVIPEFVLKELRHIADSSDSLKRNRGRRG
                     LDILNILQKDDTVNVVIESKDYDDGIEVDVKLLKLAKELGGKVLTNDFNLNKVAEFQG
                     VEVLNINELANAVKPVVLPGEEMTVMIVKDGKESGQGLAYLDDGTMIVVEGGKKCIGD
                     SVVVTVTSVLQTAAGRMIFAKLKNVINNKAV"
     gene            6368..>6696
                     /locus_tag="PLB33_05540"
     CDS             6368..>6696
                     /locus_tag="PLB33_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63020.1"
                     /translation="MIVAIRALILYAVLLIAMRIMGKGDLGELQPFDLVVSLVLAELA
                     VMPMEDLDAPLFHGLMATAVVMFLQCLISYITLKSNSARKVICGTPSVIYDHGKFNIK
                     DMNKLRI"
     assembly_gap    6697..6796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6841..7224
                     /locus_tag="PLB33_05545"
     CDS             6841..7224
                     /locus_tag="PLB33_05545"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HOG63021.1"
                     /translation="MKMVVISLIILLLMIGIWAWFYYGSVDPVTTYYWDSLIKLSDIV
                     RKEDWERAKRDISIYTDKWYEVKKTWVFFINQEDLDNIDSSIRQLNIYIENEEKILAQ
                     AELEHLIVLFYVIDENECLTIENIF"
     gene            complement(7450..8334)
                     /locus_tag="PLB33_05550"
     CDS             complement(7450..8334)
                     /locus_tag="PLB33_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOG63022.1"
                     /translation="MFLLTYSYNSVQSAGFLNEEKSGIIPASEVLQGASNIDMIVIIK
                     IFNNFNLKELKNTINTYKDAISLNEVKVLSPIPFPKRNIICLGKNYLDHISEVKSLKN
                     VNSDIPQKPIYFSKIAYPAIGHEDVVNSHSNLVKQLDYEVELAIIIGKKCKNVSMEEA
                     EDIIFGYTIANDISARDIQTTHGQWHKGKSFDTFCPIGPYIVHKSSISFPPKLKIQSY
                     VNNELRQNGNTDNLIFDIPYIISDLSSGITLYPGDIILTGTPAGVGAGFNPPRYLKPG
                     DRVDCVIEGIGTLSNTIA"
     gene            8553..13418
                     /locus_tag="PLB33_05555"
     CDS             8553..13418
                     /locus_tag="PLB33_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HOG63023.1"
                     /translation="MNRSVLKELQKEILLNLTQNEFLKSLGISENKIQSYLNNSKFTK
                     GLSTLFNKKQITCKAVLDLSADILNNEQVEPPENWLSYIFQYVLEKSFPDAVTIELNS
                     EYEISAVIYLEILRTILKYTQINNIGKVPKFEFLTEEEINDLSNKQEYLNFLDVFDKN
                     YIYELMMLDGEVNGYNTLHHVSTVHFVAMHVAKQLKKAGVQVDLGIVSGSAAGHDIGK
                     YGCKGLEKRRVAYLHYYYTDQWFKKYDMPGIALIAANHSTWDLELENLSLESLLLIYS
                     DFRVRNKITKTGEEMQIFSLAESFDIILKKLDNVDEAKEKRYIRVYSKLRDFEDYLLN
                     KGINTDLLSEEPKYVKTVDFALIDGYEVVKNFKFKAFEHNIPLMRMLNNEVTFTGMIE
                     AARSEWDWKNIRAYLNILEEYSTYLSQKEKLFALSFLYELLVHREGDIRRQAAKLMGT
                     IIIHYDMGYTKEMPEDVKITHKEKNAGLSLWDKYLGFFLTPGYKVTDKQKEWIGYSLR
                     MFVDSVINSPRNTLKEEYLEIFLKHIREDINDETARFNSLNSLLSIPLELYDKEQLDF
                     VVDFSIKHFRDTSYSIRLMAAQFLFKAVQQIKITGHTLKEILNIISEFSPDDGLCMNY
                     LKYKTAQCLNVPGTLLKKYSSLLAGNWYKTSDIFLNNLKAATPWNVKTVSIDYIMENL
                     SQRNELALLQTATHLANLVKVSAMESVRNKAGNSLVQLGPMLTIDQRNEIAFELIKGL
                     EIDEMQYAKYIPEYLGRFVMLLSPKELDEFIIDLKNIYINSSERSSALVIHTFGIMVQ
                     YYPEYKERFGEDSSVIEKRLIKILGIILGGLANFNTQVKQETFLVIGQYIFGSKILTL
                     KQKHKVFSLIYKKLLTLISEKELSELFFFNNSASFNHIYRFISDYEFFNGKFDIKENK
                     NIAFFPGTFDPFSLSHKGIVKEIRNLGYDVYLAVDEFSWSKKVQPRLIRRQIINMSIA
                     DELGVFLFPDDVPVNLSNNKDLKILKTLFPKKDIYIVVGSDVLINATAYNNEPEEDSI
                     HNFNHIVFKRAKDEITDEAVKKAEEAKKRIIGTLVELKLPVYLEDISSTQIRENIDNN
                     RDISNLIDPMAQNFIYDRNLYIREPLNKAVLRTKPFVIEIVKELSKKILDEIDHCIFN
                     DTKLFENIAEKLNFKNIRLLVIRDSKNYNEMLGFSAFHKISTSDVYSEFKSPNIANYV
                     REITSGRIIVIDGIFAAPGRIYDSMEQTLITETLSHCIKNDFTYVLYNNIITGFDSDE
                     LLETLKLQGFAKIHDKSTGKIVYGVDMKFPICLTFNLESFIKEPLNENKNVYEAISYS
                     RKRLQRAMTQLYPGSLVLSFDNDMINQILINKICSLNNVPNEMQEPRVLGEYMVVPFG
                     NVLKGMIVPNTVTKSLHTEKVYSSDATRFKIKEYPFYSSIENQIRTIKSFEKPVILVD
                     DLLHKGYRIKEIDPILKRYNINVKKIIVGIMSGRGKDLKDTQGRDADYAYYIPNLRLW
                     FNENLMYPFLGGDGIMSENENITNLIPSINLLLPFYSPMYIRGASKEAIYNLSMACLE
                     NAKHILLALEKEYKEIFERNLTVKRLGEVLLSPRLPYLGDNIYYDLNKEASGYMDVNI
                     ETLLKLERIIK"
     gene            13418..>14532
                     /locus_tag="PLB33_05560"
     CDS             13418..>14532
                     /locus_tag="PLB33_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HOG63024.1"
                     /translation="MFYYKYNDKYLFSIKDEYSFTKINSIPHDCQELYYLNNLTGNSK
                     RAFSVNHPSDLYINEESLDMLRVNDISYDLPENITELIDKRKIKAVNTAYSDYADCFY
                     MDEFKKRKVNILALGDVGSTLLIGLKLLGKDSISSIGIYDRSLDKIKRWEYEMNQTSF
                     PFDDSLPTVQGINKEELFDCDMFIFCASKGVPPVGSQVADVRMAQFEGNAELIKEYAL
                     MAADCRFKGIFAVVSDPVDQLCQTVLRESRGVLAPEQIKGYGLGVMNARALYYSKKFN
                     KFSMYLKEGRAFGPHGKDLIIANSIRDYDDELSRELTKYAVEANLEVRKTGFKPYIAP
                     ALSSGAISIIYTLEGKYHYSSNYLAGVYMGAKNRNLPS"
BASE COUNT         5077 a         2124 c         2983 g         4248 t
ORIGIN      
        1 ttgcaggatg taaccagcaa gaggagcatt tattattatt tatttttaat aagactggca
       61 aaacttatga ataataaggt tgtaatgctc agtcaaggta tcggtcccat aataaacgaa
      121 aaaagcatta aggcagttaa aaacaccttg tcattggttg actacataac cgtgagagat
      181 aaacattcca aggaattttt ggaaagtata ggagtggatg aaaggaagat ttttctttca
      241 acagaccctg taataaatat caggaaagac gattccttag aatatgaaaa gcaaagtgta
      301 aggaaagtct gcttttcttt aaggaattgg aaggattcgg atgtcagcgt aaaaataagc
      361 aaggttgccc aaaggcttat agatgatgga atagaatgcc atttcatacc tttttattat
      421 aaggaagatt tgcagcttat tgcccaggtt gaagaattat taggcagtaa ggctgtatat
      481 tataaggaga ggctttcaac aggtgatgct tttgacatta ttaaaaccat ggatgtgatg
      541 gtaggggtga ggttacattc gcttatcttt gctgcggctg ccaatgtacc tatcgttgcc
      601 gtatcatatg accagaaggt tgataacttt gtaaacagcg taaatatgaa aatagcttgt
      661 acaattgata atattgatga aaataaaatt tatgatgaaa taatcgataa aataaataac
      721 gaacgtgaag aaaagataat gctgttaaaa agcgttgaaa aattaaggga attaaccaac
      781 attaattata aaattctgaa ggaaataaca aatggataaa ttaagtataa tgggtgttag
      841 aattaataat atatccatga atgaagtttt aaaactggca gaacaaaaga ttaaaaatga
      901 tgaaaagtac ataatttata ctccaaatac ggagataatc atgatgtgcc aaaaggatga
      961 agaattttta aatataatga ataaatcgaa catcaatgtt cccgacgggg tgggtttgat
     1021 ttatgctgga aaaattaaaa aattcccctt aaaagaaaag gtggcaggtt atgatttgtc
     1081 gataaatttg ctgaaaatgg cagatgaaaa aggtcttaag ctgtatgccg tggggggaag
     1141 accgggagtg gctgaagctg ccatgcaaaa tgttcaaaag aaatatcccg gaattaagat
     1201 tgtgggagca catcacggat attttaaggg tactcatttg ggaaaattcg gccacgagga
     1261 agaaatagct gttatagaag atataaacag gcataagcct catattctgt ttgtcggatt
     1321 cggggctaaa aagcaggagc agtggataga atataacaag gatttaataa atgccaatat
     1381 tattatagga aacggcggaa cactggatgg tctggcggga atcgtgaaaa gagcgccgga
     1441 catatttata aacttaggct tggagtggct ttacaggctt attaaggagc caaagagaat
     1501 taccaggcaa atagtactgc ctgtatttat gtttaaagtt attttcggca acaaagatat
     1561 tgttaaaaaa atagaataaa agggtaatat taatacaaat cgaagatttg tcatcctgag
     1621 cgatagcgaa gtatcttagg tcaatgtcat cctgagtgaa acgaaggatc tcatgagatt
     1681 cttcgagcta aagccctcag aatgacaaaa atgttattca taacaatgac actctgtggg
     1741 ttattgataa aaatgatgta aataataaaa taggaggaaa aaatgaaatt atttatcgat
     1801 acggctaatg ttgaagaaat aagaaaagct aatgatatgg gggttatttg cggagttact
     1861 acaaacccgt cattaatagc gaaggaagga agaatattta aggatgtggt aaaagaaata
     1921 accgatatag ttgacggccc gataagtgct gaagttatca gcttggactc ggaaggaatg
     1981 ataaaagaag ctgtagaact ttctaaaata cataaaaata tagttattaa aattcctatg
     2041 actgctgaag ggttaaaggc agtaaaggtt ttgagcaagg aaaaaataaa aaccaatgta
     2101 accttgatat tttctgcagg tcaggcactt ctggccgcaa aagcaggcgc aagctatgtc
     2161 agtccttttg tgggaaggct tgatgatata ggtaatgacg gaatgaatat tattaaagaa
     2221 acagttgaaa tttttaaaaa ttataatatt gataccgaaa ttatagctgc aagcataagg
     2281 catccgatgc atgttattga ggctgcaaag ttaggatgcc atattgcaac cattccttat
     2341 aaggtaataa tacaattgac aaaacatcct ttgactgata agggaataga ccaatttttg
     2401 aaagactggg atacggtacc taaataaact tggaggaaaa aatgaaatac gatgagttaa
     2461 aagtcatagc caatagtatg agaaaaaata tattgactat gatttataag gctaagtcag
     2521 gccatccggg aggctctctt tctgcggtgg aaataatggt gtatttgtac tacaaggaaa
     2581 tgaaaataga ttctccaaca gatgaaaaca gagacaggtt tgtattatcc aagggacatg
     2641 gagctcccgt tttgtatgcc gtattgatgg aaaaaggctt tataagcaag gatttaattc
     2701 ccactctaag acagattgac agcaagcttc aaggtcatcc agatatgaag aaacttcccg
     2761 gagtcgaagc ttcaaccggc tccttagggc agggactttc tattgcaaac ggcatggctc
     2821 tggcatttaa actagacaaa aagccaaaca gggtatttgc acttcttgga gacggtgaga
     2881 taaacgaggg tatgatttgg gaagcagcaa tgcttgcaag ccactacaat cttgataatt
     2941 taaccgccgt attggaccat aacggtttgc agatagatgg caaaaatgaa gatgttatga
     3001 caattgagcc gattgacaaa aaatttgaag cattcggatg gcatgtgata aaaacagaag
     3061 ggcatagttt cgaatctttg gaaaaagctt ttgaagaaag aaaaacaata aaagggaaac
     3121 cggcaataat aattgcaaat actatcaagg ggaagggttg ctctttcatg gaaaaccagg
     3181 caggctggca tggaaaggca ccgaatgaag gcgagttttg caaagcaatg gaggaaatgg
     3241 gagacatcgg agacaaaacg gaaggaggcg gatataatgg ctaaggcaac cagagaagca
     3301 tacggtgaag ctttaaaaaa attagcagca tcaaatcctg atatagtcgt attggatgcg
     3361 gatttatccg gctccactaa gacatcggaa tttaaaaagg taagtcccga aagatttttc
     3421 aatgtgggaa ttgcagagca gaatttgatc ggtacggcag cagggctgtc attggcaggt
     3481 aaaattccgt ttgcaagctc ctttgcaatg tttgctgcag gaagggcatt cgaaattatt
     3541 agaaatactg ttgcatatcc tgcattaaac gtgaagattg cagctaccca tgcaggactt
     3601 accgtgggag aagacggagg ctcacaccag gcgattgaag atataagttt aatgagaact
     3661 attcccaata tggttgttat aaatcccgcc gacagcaggg aggcagaaca ggctgtttta
     3721 aaagccgccg aatatgtggg acctgtttat atcagacttg gcagaatggc agttgaagat
     3781 gtatatacgg attcatatgt atttgaatgg ggaaaaggag tcttattaag agacggcaag
     3841 gatgtttcca taattgccac cggtcttatg gtgcaggaag cattgaaagc ccacgaaatt
     3901 ttaaaagaag acggaataaa tgccaaggtt attaatatcc atacaataaa acccatagac
     3961 agggatttaa taatcagggc agccaaagaa accaaggcaa tagttaccgc cgaagaacac
     4021 agtgtaatcg gaggtttggg aagcgctgtt ttagaagttc tgtccgatga atatcctgtt
     4081 cctttaaaaa gggtgggaat tttggacaca ttcggagaat caggaaagcc aaaagatctt
     4141 ttgcaaaaat atagattgac tgctgatgaa attgtaagac aggtaaaaga agtaattaaa
     4201 ttttaagcgc ttaaaatttc gaatgccccg ttataggggc attcttatat ttatgaaaaa
     4261 aacgtaaaat attctttaat aaaatttaaa gtaaaaatta tttttattgt tgacaagcat
     4321 gcggtatcac gatataatat tcgatttgac atttgttgcc taatgaggta taatgtattg
     4381 ataggaggtg cagcatgttt aatttaggcg acaaagtagt ataccccatg cacggagccg
     4441 gtgtaattga aactatggaa tccaaggaaa tcttaggtga aattaaaaac tattatgtac
     4501 tgaaaatgcc cataggggaa atgaaattga tgatacctgt ggataatgtg aataatatag
     4561 gtttaagaaa catcatcgat aaatatgcag tagaggaagt atataaggtt ttaaaacaaa
     4621 aagcagaaat taatgactct aactggaata agagatatag ggataacatg acaaaaatga
     4681 agacgggaga tattttcgaa gttgctcagg ttgtaagaga tttaacctac agggacaggg
     4741 aaaaaggcct ttcaaccgga gaaaagaaaa tgttagtaag tgcaaaacaa atgctcataa
     4801 gcgagattac gcttgcaacc ggcatggaca gtgaggagat acaggattat atggacgaaa
     4861 ttataaactt tgatgcatta gaatagtaaa attgctctaa tgcatttttt ctgcatcaga
     4921 atttttggtg catacattta gccggatgtc aaatttaata gaatattaat gaattcttat
     4981 tgtattattt cgataatagt tatagccaaa tatcaaaaaa tatgttacat tataaataat
     5041 actaaataat ataactttta atggttatac ttgtaaggag gtgataatat gattgataag
     5101 attgtaaaag cggttatagc tgttataggt gcatctctgg gagcagtatc gggtgtattg
     5161 ttaaataaca gcggtcttct tgaattacca agtttgcaaa gtacattagt aattataggt
     5221 attagtttag tttttggaat tatatttttt cttctttctt ctaaaattaa aaagaccggt
     5281 ttgaaaataa tagaagctct tgagaccgaa atattgaaat tttccaccat ggacatagtg
     5341 ctgggatcat tagggctgat agtaggattc ataatagcat ttcttataag ccagccccta
     5401 tccgacatta agtatgtagg tactattatt tcagttattt catttgtatt tttcggatat
     5461 ctgggtgtaa agattgctac acgaaaaaaa gacgatgttt tatggcctca aatattaaat
     5521 ttaaaaaaga gctcaacggt taaaaaacaa gagaagaata aaaaagaaat aatatctcca
     5581 aaaattcttg ataccagtgt aattattgac ggaagaatat ctgatatttg caaaacagga
     5641 ttcatagaag gaaccttagt cattccggaa tttgtattga aggaactcag acatattgca
     5701 gattcctctg acagccttaa gcgcaacagg ggaagaagag gtttggatat tttgaatatt
     5761 cttcaaaaag acgataccgt aaatgttgtt attgaatcaa aagactacga tgatggtatt
     5821 gaagtagatg taaagctttt aaaattggca aaagaattag gcggcaaggt gctgacaaat
     5881 gactttaatc taaataaggt cgctgaattt caaggggttg aagtgttgaa catcaacgag
     5941 ctggcaaatg cagtaaagcc tgtcgtactg cccggagaag aaatgactgt aatgattgtc
     6001 aaagacggaa aggaatcagg gcaaggattg gcctacttgg atgacggtac catgattgta
     6061 gtagaaggcg gcaagaaatg catcggcgat tcggtagtgg ttaccgttac cagtgttttg
     6121 cagactgcag caggaagaat gatttttgca aaattgaaaa atgtaattaa caacaaagca
     6181 gtttaatgac aaggggacgg gggtattgtc acaatctcca agaaaattcc cataggacgc
     6241 atcgaaaggt gtgtcctttt tgtgtgccct aacccagttg tcgagcttcc cgtgaactat
     6301 attaaacgta tattatttaa gggtttcggt aaaaatagtt tatgattgcg ttttgggagg
     6361 tgtcattatg attgttgcaa taagagcctt aatattgtat gcagtcttgc tgattgcaat
     6421 gcgcataatg ggaaagggtg atctaggaga gctgcagcct tttgacttgg ttgtatcatt
     6481 agtattggcg gagcttgccg taatgcctat ggaagatttg gatgctccct tatttcatgg
     6541 tttaatggct actgccgtcg ttatgttctt gcagtgttta atatcatata ttacactcaa
     6601 aagcaattcg gcaaggaaag ttatatgcgg aactccttcg gtaatttatg accatggaaa
     6661 gtttaatatt aaagatatga ataaactaag gattaannnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnntaca ttgatgaaaa tgacaaattt gttttttata agaggtaatt
     6841 atgaaaatgg tagttatttc gttgattata ttgctattga tgataggtat atgggcatgg
     6901 ttttactatg gctcggtaga ccctgttaca acatattact gggatagttt aataaaatta
     6961 tctgatatag taagaaagga agactgggaa agagctaaga gagatatttc gatatacacc
     7021 gataaatggt atgaagtaaa aaaaacttgg gttttcttta taaaccaaga agacctcgac
     7081 aatattgact cctccatacg gcagcttaat atttatattg aaaatgaaga aaaaatattg
     7141 gctcaagcag agcttgagca cttaatagta ttattttatg taattgacga aaatgaatgt
     7201 ctaactatag aaaacatctt ttgatgacag catccaaatc tggttgtgta tacgcttttt
     7261 ctcattactg tgaataagct atgtgtcatt ctgagagctc cagctcgaag aatctcatct
     7321 tataaaacct gagattctga gatcctgaga tcctgagatc cttcgctacg ctcaggatga
     7381 caatttttga cgcctaccgt cctccaggat gacaattttt gacgcctacc gtcctctttt
     7441 cattgtaatt taggctatgg tgttggaaag ggttcctata ccttcgataa cacagtcaac
     7501 tctgtctcct ggttttaaat acctcggagg attgaatcct gctcccacgc ctgccggagt
     7561 tcccgtcaaa attatatctc cgggatacag tgttattcct gaagacaggt cgcttatgat
     7621 gtatggtata tcaaatatca aattatcggt attgccgttt tgtctgagct cattgtttac
     7681 atagctttga atttttaatt tgggagggaa tgatatactt gatttatgca caatgtatgg
     7741 tcctataggg caaaaagtat caaaggattt gcccttgtgc cattgaccgt gtgttgtttg
     7801 aatatctctt gcggaaatat cgttagcgat ggtatatccg aatataatgt cttctgcttc
     7861 ctccatggaa acattcttgc attttttgcc tattattatg gctaattcaa cttcatagtc
     7921 tagctgttta acaagattgg aatgagaatt aacaacatct tcatgaccga tggcaggata
     7981 tgcaatttta ctgaaataaa tcggtttttg cggtatatcc gaattaacgt tttttaagct
     8041 ttttacttcg ctgatatggt ctaaatagtt tttacccaaa caaataatat ttcttttagg
     8101 gaagggaatg ggagataaaa ctttaacttc gtttaagctt attgcgtctt tgtatgtatt
     8161 gattgtattt tttaattctt ttaaattaaa attgttaaat attttaataa taacaatcat
     8221 atctatattc gatgcacctt gtaaaacttc tgatgcagga ataatgccgc ttttttcttc
     8281 attcagaaaa ccagccgact gtacgctatt atacgaataa gttaataaaa acataaaggc
     8341 ctccaatatt ttattctata tagactataa tataataaga atactttttc aagataaaag
     8401 tggatgtcca gcggggacgg ttctcggtgg acaaaggatg acactacccc cgtccccttg
     8461 tcataggaat gtccccgtta gtacaacttt caattgcagt accaatgaga gtatgttata
     8521 atttcgtaaa ttaaattaaa agggaggtta caatgaacag gtctgtactt aaagaattac
     8581 aaaaagaaat tttacttaat cttactcaaa atgaattttt gaaaagtctg ggtatttccg
     8641 aaaataaaat acaatcttat ctcaataatt caaaattcac gaaaggctta tcaaccttat
     8701 ttaataaaaa acaaataacc tgtaaagctg tacttgacct gtcagcagat attttaaaca
     8761 atgagcaagt tgaacctcct gagaactggt tatcatatat atttcaatat gttttggaaa
     8821 agtcctttcc ggatgccgtt acaatagagc tgaattccga atatgaaatt tcagctgtca
     8881 tatatcttga aatattgaga actattttaa aatatacgca aataaacaat atcggtaaag
     8941 ttccgaaatt tgaattttta actgaagaag aaataaatga cctttcaaac aagcaggaat
     9001 atttgaattt tttggatgtg tttgataaga attatattta tgaacttatg atgcttgacg
     9061 gggaggtcaa cggttacaat actttgcatc atgtttcgac agtacatttt gttgcaatgc
     9121 acgttgcaaa gcagcttaag aaggccggag tccaggtgga cttaggcatt gtttccggct
     9181 ctgccgcagg ccacgacatt ggaaagtacg gttgtaaggg attagaaaaa agaagggttg
     9241 catatcttca ctattattat accgaccagt ggtttaaaaa gtacgatatg ccggggattg
     9301 ctttaattgc agcaaatcat tccacttggg atttggagct tgaaaactta tccctggaat
     9361 cattattgtt aatatattct gattttagag taaggaacaa aataaccaag accggtgaag
     9421 aaatgcaaat tttttcattg gcggaatcat ttgacatcat tcttaaaaag cttgacaatg
     9481 tagatgaagc aaaagaaaaa agatatataa gagtatattc caaactaagg gattttgaag
     9541 actatttatt aaacaaagga ataaatactg accttttatc ggaagagcct aagtatgtaa
     9601 agacagttga ctttgcccta atagacggat atgaggtggt gaaaaatttt aaatttaagg
     9661 cgtttgagca caacattccc ttaatgagaa tgcttaacaa tgaagttaca tttacaggaa
     9721 tgattgaggc tgccagaagc gagtgggatt ggaaaaatat ccgtgcatat ttaaatattc
     9781 tggaggaata ctccacatat ttgtctcaga aggaaaagct gtttgcttta agtttcctgt
     9841 atgaactatt ggttcacagg gaaggagata taaggcgtca agcagcaaaa ttgatgggaa
     9901 caattattat tcattatgat atggggtata caaaggaaat gcctgaagat gtgaaaataa
     9961 cacacaagga gaaaaatgcg ggtttatcct tgtgggataa gtatttggga tttttcctta
    10021 ctcctgggta taaagtaacg gacaaacaaa aagaatggat tggttattct ttaagaatgt
    10081 ttgttgattc agtgataaac tctccaagaa acacattaaa ggaagaatac ctggagatat
    10141 ttttaaaaca tatccgtgaa gatataaatg acgaaaccgc aaggtttaac agtttaaact
    10201 ctcttttaag cattcctttg gagttatatg ataaagagca gcttgacttt gtggtggatt
    10261 tttcgataaa gcattttaga gatacatcat acagcataag gctgatggca gcacaatttt
    10321 tattcaaggc tgtgcaacag ataaagatta cgggacatac tcttaaggaa atactcaata
    10381 ttatatcaga gttttctccc gatgacgggc tgtgcatgaa ttatttgaaa tacaagacag
    10441 cgcaatgttt aaatgtaccc ggtacattgc ttaaaaaata ttcatctctt ttagcaggca
    10501 attggtataa aacatcggat atatttttaa ataatttgaa agctgcaaca ccctggaatg
    10561 taaaaaccgt aagtattgat tacataatgg agaatctttc ccaaagaaac gaacttgcgc
    10621 ttctccagac ggcaacacac cttgcaaatc ttgtaaaggt tagtgccatg gaatcagtga
    10681 gaaacaaggc cggtaactct cttgtacaat taggtcccat gcttaccata gaccaaagaa
    10741 atgaaatagc atttgagctt attaaaggtc ttgaaataga cgaaatgcag tatgcaaagt
    10801 atattcccga atatttgggg cgctttgtaa tgttgttgtc tccaaaagag ttggatgagt
    10861 ttattattga tttaaaaaat atttacatta attcaagcga gcgctccagt gcattggtaa
    10921 tacacacttt cggaataatg gttcaatatt accccgaata taaggaaaga tttggagaag
    10981 acagcagcgt tatagaaaaa agactcatca aaattttagg gataattttg ggagggcttg
    11041 ctaattttaa tacacaggta aaacaggaaa catttttggt aatagggcag tatattttcg
    11101 gctctaaaat attgactcta aagcagaagc ataaggtgtt ttcacttatt tataagaagc
    11161 ttttaacatt aataagcgag aaggaattaa gcgagctgtt ctttttcaat aactccgcct
    11221 catttaacca catatacagg tttataagcg attatgagtt tttcaacggt aaattcgata
    11281 taaaagaaaa taaaaatatt gcgtttttcc ccggaacctt tgaccccttt tcgctgagtc
    11341 acaagggaat cgtaaaggaa ataagaaatt taggatatga cgtatacttg gctgtagatg
    11401 aattttcctg gtctaagaag gttcagccca gattgataag aagacaaatt atcaatatgt
    11461 caattgcaga tgaattaggt gtatttttgt ttcctgatga tgtacccgta aatttatcaa
    11521 acaataaaga tttaaaaata ctaaaaacac tttttcccaa aaaggatatt tatatcgttg
    11581 taggaagcga tgtattaata aatgcgacag catataataa tgaacccgaa gaagattcaa
    11641 tacataattt taatcacatc gtatttaaga gagcaaagga tgaaattacc gatgaagccg
    11701 ttaaaaaggc agaggaagca aagaaaagaa taataggaac acttgttgaa ttaaagcttc
    11761 ctgtttattt agaggatata agctcaaccc aaataaggga aaatatagac aataacagag
    11821 atatatcaaa cttaatcgac cccatggctc aaaatttcat atatgacagg aatttgtaca
    11881 tcagggagcc tttaaacaaa gcagtactga gaacaaagcc ctttgttatt gagatagtta
    11941 aagagttgag caaaaaaatt cttgatgaaa ttgaccattg tattttcaat gatacaaaac
    12001 tgtttgaaaa tattgcagaa aagcttaatt tcaaaaatat tagactgctt gtaataaggg
    12061 attccaaaaa ttataacgag atgcttggat tttctgcatt tcataaaata agcacctctg
    12121 atgtttattc cgagtttaaa agtcccaata ttgcaaatta tgtgagagaa ataacatccg
    12181 gaagaattat tgttatcgac ggaatatttg cggctcccgg caggatatat gacagtatgg
    12241 agcaaaccct tattacggaa accttatctc actgcataaa aaatgatttt acctatgtct
    12301 tatataataa tattattacc ggctttgaca gcgatgaatt gttggaaaca ttaaaacttc
    12361 aaggatttgc caagattcat gacaaaagca ccggaaagat tgtttacgga gtagatatga
    12421 agtttcccat atgcttgaca tttaatctgg aaagttttat taaggagccc ttgaatgaaa
    12481 ataaaaatgt ttatgaagct atttcttatt cgagaaaaag gcttcaaaga gcaatgacac
    12541 agctgtatcc cggaagtctt gtattgtcat ttgacaatga tatgataaat caaattctta
    12601 ttaataaaat atgcagttta aacaatgtgc ccaatgaaat gcaagaaccg agagtattgg
    12661 gcgaatacat ggttgtgcct ttcggcaatg ttttaaaggg tatgattgtt ccaaacacag
    12721 taactaaatc attgcatacg gaaaaagtat attcttccga tgcaactaga ttcaaaatta
    12781 aggagtatcc gttttactca tctatagaaa atcagataag aaccataaag tcttttgaga
    12841 aaccggtgat tttggttgat gatttacttc ataagggtta caggattaaa gaaatagacc
    12901 ctatattgaa gcgttataat ataaatgtca aaaaaatcat tgtgggtata atgtccggca
    12961 ggggaaagga tttgaaggat acacaaggaa gagatgcaga ctatgcttat tatatcccta
    13021 acttaaggct ttggtttaat gaaaatttga tgtatccttt tttgggagga gacggcataa
    13081 tgagtgaaaa tgagaatata acaaatttga ttccttcaat aaatttattg ctgccctttt
    13141 attcgcccat gtatataagg ggagcttcca aagaagcaat atacaatctt tctatggcct
    13201 gcctcgaaaa tgcaaaacac attcttcttg cattggaaaa ggagtataag gaaatttttg
    13261 aaaggaacct gactgttaaa aggctgggtg aagttctttt gtcaccgaga ctgccctact
    13321 tgggagacaa tatctattac gatttaaaca aggaagcatc cggatatatg gatgtaaata
    13381 ttgaaacact tttaaaactt gaaaggatta taaaataatg ttctattaca aatacaatga
    13441 taaatattta ttctcaataa aagatgagta tagttttaca aaaataaata gcattcctca
    13501 tgactgccaa gaattatatt atcttaataa cttaaccgga aattcaaaac gcgctttttc
    13561 cgttaaccat ccttccgact tgtacataaa tgaggaaagc ctggatatgc ttagagtaaa
    13621 tgacataagt tatgaccttc ccgaaaatat tactgagctg atagacaaaa gaaaaattaa
    13681 ggcagtcaac accgcctaca gtgattatgc tgattgcttc tatatggatg aatttaaaaa
    13741 aaggaaggta aatattcttg ccctcggtga tgttggaagt acattactta ttggacttaa
    13801 acttctcgga aaagattcaa taagctccat cggtatttat gacagaagtt tggataaaat
    13861 aaaaagatgg gaatatgaaa tgaatcaaac atcatttcct tttgatgatt cgctgcccac
    13921 ggttcagggt ataaacaagg aagaattatt tgattgtgac atgtttatat tttgtgcttc
    13981 caagggggtt cctcccgtag gaagccaagt tgccgatgtt aggatggctc aatttgaagg
    14041 aaatgcagag cttattaagg aatatgcctt aatggcggca gattgccggt ttaagggaat
    14101 atttgcagta gtttcggacc ctgtagacca attatgccaa acagttctaa gagagagcag
    14161 gggtgttttg gcacccgagc aaattaaggg gtacgggctt ggagtaatga acgcgagagc
    14221 tttgtactac tcgaaaaaat tcaataaatt ttcaatgtat ttaaaagaag gaagagcatt
    14281 cggaccgcac gggaaagact tgataatagc aaacagtata agagattatg atgatgagct
    14341 ttcaagagaa ttgacaaaat atgcggttga agcaaacttg gaagtaagaa agacgggatt
    14401 taagccatat attgcacctg ccctttcatc cggagcaatt tcaataattt atactctcga
    14461 agggaaatat cattacagct ccaattattt agccggagtt tacatgggag ctaaaaacag
    14521 aaacctgcct tc
//
LOCUS       DAOSHP010000042        14130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3575_length_14130_cov_9.475524, whole genome shotgun sequence.
ACCESSION   DAOSHP010000042 DAOSHP010000000
VERSION     DAOSHP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3575_length_14130_cov_9.475524"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..72)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(147..1143)
                     /gene="guaA"
                     /locus_tag="PLB33_05565"
                     /pseudo
     CDS             complement(147..1143)
                     /gene="guaA"
                     /locus_tag="PLB33_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965987.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     assembly_gap    1268..1367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1385..2833)
                     /gene="gcvPB"
                     /locus_tag="PLB33_05570"
     CDS             complement(1385..2833)
                     /gene="gcvPB"
                     /locus_tag="PLB33_05570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOG63025.1"
                     /translation="MQLIFEKSKKGRKAYSLPECDVPDVSLDVIDKKYLRAEAPELPE
                     VSELDAVRHFTQISQRNHGVDSGFYPLGSCTMKYNPKVNELAARFDGFTKIHPYQQEK
                     TVQGALKLMYDLGRMLSEITAMEEFTLQPAAGAHGELTGLMIMKAYHHNRNNEKRTKI
                     IVPDSAHGTNPASAAVAGFDVVEIKSNDKGLVDLEELKAAVGEDTAGFMLTNPNTLGL
                     FDENILEISKIVHDAGGLMYYDGANANAILGISRPGDMGFDVVHLNLHKTFSTPHGGG
                     GPGSGPVGVKKHLAEFLPVPVISKKEEEYYMDYERPNSIGKVKSFYGNFGVLVRAYAY
                     ILSMGPDGLKEVAENAVLNANYIANKLKDKYFLPIDTECMHECVFSADWQKEKNGVSA
                     LDIAKRLLDFGFHPPTIYFPLIVHEALMVEPTETESKETLDEFISVMRQIAEEAENTP
                     EMVKTAPHTTVISRLDEATAARKPILRYTRLT"
     gene            complement(2835..4172)
                     /gene="gcvPA"
                     /locus_tag="PLB33_05575"
     CDS             complement(2835..4172)
                     /gene="gcvPA"
                     /locus_tag="PLB33_05575"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOG63026.1"
                     /translation="MTRYIGNTTDDRQQMLNEIGYKDIDSLFRAVPDSVRLKEKLKIP
                     EALSEMELIKKIKSLSDKNFNSDEYVCFMGAGAYDHYIPSAIDQLIMRQEFYTAYTPY
                     QPEISQGTLQAIFEYQTMISELTGLPVANASMYDGATALAEASAMSCEATRRKEVLVA
                     SSVHPESRRTLNTYAKFRNITVKEISYENGQISLEELNTKINDNTAAVIIQNPNFFGI
                     IEDVEKIAEITHKNKSLLVLSADPISLSILKSPGEMGADIAVGEGQPLGNSLNFGGPY
                     LGFMAVTEKLMRKMPGRIVGQTTDKEGRRGFVLTLQTREQHIRREKATSNICSNQALN
                     ALQATMYLTFMGKEGLKKVAKLCLNKSHYAYSELIKTNKFKPLFTAPFFKEFAVISDK
                     PVSDLNEKLLKSNIIGGYNIEIDYPELKNAYLLAVTEKRTKEEIDALVRKAGE"
     gene            complement(4183..4557)
                     /gene="gcvH"
                     /locus_tag="PLB33_05580"
     CDS             complement(4183..4557)
                     /gene="gcvH"
                     /locus_tag="PLB33_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOG63027.1"
                     /translation="MKLLPELKYVESHEWVRVEENKAYVGITDYAQEQLGDIVYVEVP
                     EVGTVVEAGDQFVVLESVKAASDVYAPVSGTVVEVNEELEDNPALINESAYDAWIVAI
                     DMSMPTEVDSLLSAEEYEELCE"
     gene            complement(4642..5742)
                     /gene="gcvT"
                     /locus_tag="PLB33_05585"
     CDS             complement(4642..5742)
                     /gene="gcvT"
                     /locus_tag="PLB33_05585"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOG63028.1"
                     /translation="MKKTPLYEKHIQLKGKMIDFGGWSLPVEYTGIIPEHEAVRTKAG
                     IFDVSHMGEITVKGEDSEKYLQMLLTNDISLLNNNQIAYTAMCYPHGGVVDDLLVYKY
                     SNTDYLLVVNASNTEKDYQWMKENIFGNTEILNVSENYAQLALQGPLAETILQKLTSK
                     NLNEIEFYYFSDNVKVGNIEALVSRTGYTGEDGFELYFAYDKAEEMWDLILEAGKDEG
                     LIPAGLGARDTLRFEASLPLYGHELNENITPLEAGLGFFVKLNKENFIGKEALARQKS
                     EGLKRRIVGFEMIDRGIPRNNYEVYKEGEKIGYVTTGSFSPTLKKNIGLALIDSAYSK
                     EGTEIEILIRNKNSKAKVIKKPFYSKKYKKMA"
     gene            complement(5967..6320)
                     /locus_tag="PLB33_05590"
     CDS             complement(5967..6320)
                     /locus_tag="PLB33_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63029.1"
                     /translation="MVIKKFSFSNKNNDEKLLNWEDSVKLDSQQKLREQMKQMNNENK
                     NRNKNDNRFDNRNDNKFDNRNENKFDNRNDNRNDNNKFDSRNDDDWKSLNKNNQDNKN
                     NNKNKNKNSQNNNLK"
     gene            complement(6465..7403)
                     /locus_tag="PLB33_05595"
     CDS             complement(6465..7403)
                     /locus_tag="PLB33_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOG63030.1"
                     /translation="MDNFIFSLNVVLPLVFLMFIGSFLRRINIFDEDFLRKLNKITYC
                     VLLPVLLFNNIYTSKVSGEIDLKFIIFFVILALAVIGILLIVIPKYEKDNRNRGVLIQ
                     GLFRGQFVFFGIPLMANISGDEGLVVLSTLIAIIVPIYNFLAVIILDLFTDERHNYKD
                     TIISILTNPLVIGSLLGIAASVLQIKLPSALNKTIADISKTVTPISLMILGGVIEINN
                     IWKNRKKLILIAAVKLFFIPSFAALITIFFGYRGIELGAVLSMIAPPTAVASYTLAQQ
                     HDCNDELAGQIVFFTTLISPFSIFLFIFVFKTFGLF"
     gene            complement(7416..8354)
                     /locus_tag="PLB33_05600"
     CDS             complement(7416..8354)
                     /locus_tag="PLB33_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOG63031.1"
                     /translation="MENLIFSLNAVLPLVFLMLLGAFLRKINVFDEDFLGKANKFIFK
                     VLFPFFLFNNIYSSKISEEIDSKFIIFVVIISIAVVGLLIVIIPKYEKDNKNRGVIIQ
                     GLYRGNTTLFATALISNIFGNEGLAIVTMLIAIISPTYIFLTVIILDLFTDERQNYKK
                     TLISILSNPLIIGSLLGIAVYTLHIKLPLAMEKTIADIAKTTTPIALMTLGGEIEINN
                     IWKNIKPLVFVVAGKLIIFPAIATLIAISYGYRGVELGVILSMTAPPTAVTSYALAQQ
                     HNCNYELAGQIVFLTTLISPFTIFLFVYIFKAAGLF"
     gene            8653..10632
                     /locus_tag="PLB33_05605"
     CDS             8653..10632
                     /locus_tag="PLB33_05605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOG63032.1"
                     /translation="MENLKLKDFLDYTFLSGLELSPDKNYGAFVVHTSDFEDNKYLSN
                     IWLYNLTTSEYKKLTNMNEEKSFIWLDNNTILFSSLRDEKLKKRIQDGEHWTVFYAID
                     IQGGEAYEYMRIPMKVNNIKKIADEKFLLTAIYDHYGINLHSRKGEEKTKAIELIKEN
                     KDYEILDEIPFWSNGEGFINKKRNRLYLFDKGTKDIVPISNQYENIKVTSVKDGKALY
                     VSSIYTNKMELTTGLYMYDLYSSKALCLVEDLEYNIEFAEFVGDDIIVAATTMDKYGI
                     NENFNFYKLQDGELVLLSKHDFGLNNSVNSDCRYGEGAARRVFDNCLYFISTENKSSF
                     IKKLSLDGSIEKLTEDNGSVDCFDICSQGIIFIGLRGSRLQEIYTLQKIKETQRTKFN
                     EKIVEIKTIVKPESINFQNDGVTIEGFVLKPVNFDEKKCYPGILNIHGGPKTVYGEVF
                     YHEMQYWANEGYFVFFCNPRGSDGRGDEFADIRGKYGTIDYDDLMKFTDKVLEKYPQI
                     DCTRIGVTGGSYGGFMTNWIIGHTDRFKCAVSQRSISNWISKFGTTDIGYYFNTDQNL
                     SSPWDNVEKMWFHSPLKYANQAVTPTLFIHAEEDYRCWLAEGLQMFTALKYHGVDARL
                     CMFRGENHDLSRSGKPRHRVRRLEEITNWFEKYLR"
     gene            complement(10780..10989)
                     /locus_tag="PLB33_05610"
     CDS             complement(10780..10989)
                     /locus_tag="PLB33_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63033.1"
                     /translation="MFNNILGDSGDSLLFFFLLLVVLACFCGDDGLVGGIGGFGDNDS
                     LLFFFLLLVILFCSCDRVGGIGDRC"
     gene            complement(11052..11339)
                     /locus_tag="PLB33_05615"
     CDS             complement(11052..11339)
                     /locus_tag="PLB33_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63034.1"
                     /translation="MDKRKCDDNMIERMYDIIELIYILNRLTNKFCEDEHSKQYLFDI
                     MDNMQNVKYSYDKYRGKCSNRNLIKHECHNYCTKDSFIDKIIFYLKSLNEE"
     gene            11573..12943
                     /locus_tag="PLB33_05620"
     CDS             11573..12943
                     /locus_tag="PLB33_05620"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOG63035.1"
                     /translation="MRAFFNIVIIGLIGLLSSTYLFNLSPVDYKGTIEIETPVEESTL
                     SLEVEEDKILNPESITIKHTASKEEVIKIVSNSIFNVDIYKDNKLVKSNIDNLEPVSP
                     SIDATISVNKDNPIITAVNIAQKNLGLEDGVYKFVFNSNIIADIDKASVSVTVTYDTT
                     GNYYPAVNTAPAGTKGLTLYFPTKNADTLIPVTRFVVEDKSITRMAIEQLQNGPLSKE
                     LISVIKDVTNTTYNNGNVVIDLPSSYTAYNTGSTGAVLAYESFVKTIFAVDRYWPIHS
                     VTFTVDRKNVDTYFHGMSREAINYLPNIKRNYLLYLAHKADNRYYLFEYQLSPEEIGI
                     AENDSIEMKARKIFDAYSNTDIEYGISPVPKTVTLNNVSLQGRTLILDFNKDFLNVYE
                     DKNDLRHMMIESFLYTFTTIPGVDSIKITAENKEITDFIDVLDTTKILYPPEFINPEA
                     VEEKEE"
     gene            complement(13110..13631)
                     /locus_tag="PLB33_05625"
     CDS             complement(13110..13631)
                     /locus_tag="PLB33_05625"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOG63036.1"
                     /translation="MDDMKVKDMVYTALFAALVCVATFIIKVPLPVSGGYAHIGDGFI
                     FLAVILIGKKNGAWAGAIGAALADIIGGYSIYALPTFIIKGVMALIMGYMIEKLPSLK
                     HNWVAGAITGSIWQVLSYYVVGSLMVGNFLTTIGEIPGNTAQSVVGIIVSAAFLEVFK
                     HTPVKGVGTHLLR"
     gene            complement(13815..14078)
                     /gene="ffs"
                     /locus_tag="PLB33_05630"
     ncRNA           complement(13815..14078)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLB33_05630"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         4389 a         2625 c         2252 g         4764 t
ORIGIN      
        1 cataaagaag attcacccca aaaagttggt tcccccgctc catccacatg ttggactcag
       61 cgttgtataa aaagaaaaat tatttaatta gttttataat tataatctaa aagtatatta
      121 aagtcaagaa ataaaaatcg actaatttat tcaaattcaa tcgttgcagg cggcttagca
      181 gtgatatcat atacaactct gtttacattc tcaacctcgt taacaattct gctgcttact
      241 ttttccaaga cttcataggg tattcttgcc cactcggaag tcatgccgtt tgtagaatga
      301 actgctctta gggcaattgt gtaacaatat gttctttcat ctccggttac gcctacgctt
      361 ttgatgtcgg gaagggctgc aaagtattgc catattttat tttgaaggcc tgcttttttt
      421 atttcatctc tgaaaataaa gtcagcttct cttactattt ttaatttttc ttcggttact
      481 tctcccaata cacggattcc aagacccggt cccgggaatg gctgtctttc aactatctct
      541 tcaggcagac ccaactctct tccaactagc cgaacttcat ctttatacaa ttgtctcaga
      601 ggctcaataa gttcaaagtc aacatcatca ggaagccctc ccacattgtg atggcttttg
      661 attactgaag ccgcagatgt tccgctttct ataacatccg gataaatcgt gccctgaaca
      721 agatagtcga tgtgtccgaa tgtgttctta agtttctctt tttcttcttc aaacaccctt
      781 ataaactctt cgcctattat ttttctcttc ttttcaggct cagttacacc tgccaatttt
      841 ctgaggaatc tgtcctgagc attcacacga atcagattca tgtctaattt atttttaaat
      901 actttttcaa cctggtcagc ttcgtccttt cttaaaagtc cgtggtctac aaaaacacat
      961 attaaatttt tacctattgc tttgtgcacc attgttgcag ctaccgatga atcgacacct
     1021 cccgacaagg cacaaagagc ttttttgtct ccaacttgtt tttttatttg taaaattgaa
     1081 tcttcaacaa aatttctgac attccagtct tttctcattt gagtacccct aatttttaaa
     1141 gtttattaag ctttgccatc atgcaaagta tctcagactt tgagattctt cattgtgttc
     1201 agaatgaaaa gctatacctg acatcaataa gattatcaaa taaaatatat ggtgtcaata
     1261 aaaaggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt tacataattg
     1381 gtaattatgt taacctcgta tatcgaagta tgggttttct tgcagctgtt gcttcatcta
     1441 acctgcttat aactgttgtg tggggagctg tttttaccat ctccggagta ttttctgctt
     1501 cttctgctat ctgtctcatg acagaaatga attcatccag tgtttctttt gactcggttt
     1561 ccgtgggttc aaccatcaat gcttcatgaa caattaaggg gaagtaaatc gttggaggat
     1621 gaaatccgaa atccagcaat ctttttgcta tatcgagagc tgaaactcca ttcttctcct
     1681 tctgccaatc cgctgaaaac acacattcat gcatgcattc cgtgtcaatt ggcaggaagt
     1741 atttgtcctt aagcttattt gcaatataat ttgcatttaa aactgcattt tcagctactt
     1801 ccttaagtcc atccggaccc atgcttaaaa tatatgcata tgctcttacc aatacaccaa
     1861 aattaccgta aaatgatttt actttgccga ttgaatttgg tctttcataa tccatgtaat
     1921 actcttcttc ttttttagaa ataaccggta ccggcagaaa ttccgcaaga tgttttttca
     1981 ctcctacagg acctgaaccc ggaccgccgc ctccatgagg tgtactgaat gttttatgga
     2041 ggtttaagtg caccacatca aatcccatat ctcccggcct tgatatgcct aaaattgcat
     2101 ttgcattggc tccgtcataa tacatcaagc cgccggcatc gtggactatt ttagatattt
     2161 ccaatatatt ttcgtcaaat aaacctaatg tattgggatt agtcagcatg aatcctgctg
     2221 tgtcttctcc gactgcagct ttcaattctt ctaaatctac caagcccttg tcattagatt
     2281 ttatttcaac tacatcaaat ccggcaactg ccgcagaagc gggatttgta ccgtgtgctg
     2341 aatccggaac tattattttg gttctttttt cattatttct gttatgatga taagctttca
     2401 ttatcattaa tcccgtcagc tctccatgag ctcctgccgc cggctggaga gtaaattctt
     2461 ccatagccgt tatttcagaa agcattctgc ccaggtcata cattaatttt aatgcaccct
     2521 gtactgtttt ttcctgctga tacggatgaa tttttgtaaa cccgtcaaat ctagccgcca
     2581 attcgtttac tttgggatta tatttcatgg tacagcttcc gagcggatag aatccggaat
     2641 ctacaccatg atttctttga gaaatttgtg taaagtgacg aactgcatca agctcgctga
     2701 cttcaggaag ctcaggagcc tcagctcgta agtatttttt atctatcaca tctaaggata
     2761 catccggcac atcgcattca ggaagtgaat atgcctttct gcctttttta cttttttcaa
     2821 atattaattg catattactc acctgccttt cttactaatg catctatttc ttctttggtt
     2881 ctcttttcgg ttaccgcaag caggtaagca ttttttaatt caggataatc aatctcaata
     2941 ttatagccgc ctattatatt gctttttaag agtttttcat ttaagtcgct tacaggttta
     3001 tcgcttatta cagcaaattc tttaaagaat ggtgctgtaa acaagggctt aaatttattg
     3061 gttttaatta actcgctata agcatagtgg gatttgttaa gacaaagctt tgcaactttt
     3121 tttagtcctt cctttcccat aaatgttaaa tacatggttg cctgcagtgc atttaaagct
     3181 tggttagagc atatatttga ggttgctttt tctcttctta tatgctgttc ccttgtttga
     3241 agagtcagta cgaatcctct cctgccttct ttatcggtcg tctgtcctac aattcttccg
     3301 ggcatttttc gcatcaactt ttctgtaaca gccatgaagc ctaaataagg tcctccgaaa
     3361 ttaagtgaat ttccaagcgg ctgcccttca cccacagcaa tatccgctcc catttctccc
     3421 ggacttttta aaattgacaa tgaaatagga tctgcgctta agaccaaaag acttttgttt
     3481 ttatgagtta tttcggcaat tttttctaca tcttcaatta tgccgaaaaa attcgggttt
     3541 tgtataatta cggctgcagt attatcatta atctttgtat ttagctcttc taagcttatt
     3601 tgaccgtttt catatgatat ttcttttacc gttatatttc tgaatttagc atatgtattt
     3661 aaagttcgcc tactttcggg atggacagaa gatgctacta atacttcctt cctgcgtgtt
     3721 gcttcacaag acattgcact agcttccgcc aaagctgtcg caccgtcata cattgatgca
     3781 ttagcaacgg gaagtccggt taattcactt atcattgttt ggtattcaaa tattgcctgc
     3841 aatgttccct gacttatttc aggctggtag ggagtatatg ctgtatagaa ttcctgcctc
     3901 attattaact gatctattgc tgaaggtatg taatggtcat atgcgccggc acccataaaa
     3961 catacatatt catcgctatt aaaattttta tcggataatg attttatttt ttttatcagt
     4021 tccatttctg acaaagcttc aggtattttt aacttttctt ttaatcgtac cgaatcagga
     4081 acagctctga acagagaatc tatatcctta tatccgattt catttagcat ttgctgcctg
     4141 tcatcagtgg tgtttccaat ataccttgtc ataactgctc ccttattcac ataattcttc
     4201 gtattcttca gctgataaca agctgtcaac ttcagtaggc atgctcatat ctattgctac
     4261 aatccatgca tcatatgcag attcattgat taaggccgga ttgtcttcca actcttcatt
     4321 tacttctaca actgttcctg aaacgggggc atatacatct gaagccgcct taactgattc
     4381 aagtacaaca aactggtcgc ctgcttcaac tacagttcca acctcaggca cttccacata
     4441 aacaatatcc cctaactgtt cttgtgcata gtcagtaatt cctacatatg ccttattttc
     4501 ttctactctc acccattcat gagattcaac gtattttaat tctggtagta atttcataat
     4561 ttcctccgtg tatttagata ttattataga tacccaataa atatattttt aattaaattt
     4621 tttcgtatgt atagaatagg gttaggccat ctttttatat tttttggaat aaaaaggctt
     4681 tttaataact tttgccttag aatttttatt tcttattaaa atttctattt ctgttccttc
     4741 ctttgaatat gcagaatcta tgagggctaa tcctatgttt ttctttaagg taggtgaaaa
     4801 acttcctgtt gttacatatc ctattttttc cccttcttta tatacttcat agttgtttct
     4861 cggtattcct ctgtcaatca tttcaaaacc gactattctt cttttaagac cttctgattt
     4921 ttgtctagct aaggcttcct ttcctatgaa attttctttg ttcagcttta caaaaaaacc
     4981 aagtcctgct tctaagggcg taatattttc atttaattcg tggccataaa ggggcagtga
     5041 tgcttcaaat cttaatgtat ctcttgcgcc taatcctgcc ggtatcaagc cttcgtcttt
     5101 tcctgcttct aaaatcaaat cccacatttc ttcagcctta tcataagcaa aatataactc
     5161 aaaaccatct tctcccgtat aaccggtcct ggaaaccaat gcttcaatat tgcctacttt
     5221 tacattatcg ctgaaataat agaattctat ttcatttaga tttttacttg tcaacttttg
     5281 caaaattgtt tcagccagag gtccttgcag agcaagctgg gcataatttt ctgaaacatt
     5341 taatatttca gtatttccaa atatgttttc tttcatccac tgataatcct tttcagtgtt
     5401 ggatgcatta acaaccaata gataatctgt attgctgtat ttatagacta acaaatcgtc
     5461 tactactccg ccgtgaggat aacacattgc ggtataggca atttgattgt tattaagcaa
     5521 tgaaatatca ttagttaata acatttgcaa atatttttcg gaatcttctc ctttaactgt
     5581 tatttcaccc atgtgagata catcaaaaat tcctgctttt gttcttacag cttcatgctc
     5641 gggaattatt cctgtatatt caaccggcag gctccacccg ccaaaatcaa tcatctttcc
     5701 cttaagctga atatgttttt catataaagg cgtttttttc attacacact cctcttaaaa
     5761 ttaaagacag aaaatatctg tctttgtttt tgatattatt ttttattcat tatcacttta
     5821 ttaaaattat ttgtcaatat tataaaacca ataaatatcc gtgtcaatat atattgtttg
     5881 tgaaaaaaaa tacttttata tgcatattac tttcatctac cggtattaaa aaacggtcat
     5941 tgagaatgac cgtttcaaat acccaactat tttaaattat tgttctgact gtttttattt
     6001 ttgtttttat tgttgttttt attatcctgg ttgtttttgt tcagactttt ccagtcgtca
     6061 tcatttctac tatcaaattt gttgttgtcg tttcgattgt cgtttcgatt gtcgaatttg
     6121 ttctcatttc gattgtcgaa tttgttgtcg tttcgattgt caaatctgtt gtcatttttg
     6181 tttctgtttt tgttttcgtt attcatttgt ttcatctgct cccttaattt ttgttggctg
     6241 tctaatttga cactgtcttc ccaatttaat aacttttcgt cattattctt attgctaaaa
     6301 ctgaatttct ttattaccat catcccttca tatacatgta tttgaagatg tttgtcatct
     6361 acatgaatat tattagcttt ttcatattaa ttattattcc aataaatgct taatgtgggg
     6421 ttggtatcca taaattcaat tttttaactg caataataaa attactaaaa caaaccaaaa
     6481 gttttaaaaa caaaaataaa caagaaaata ctgaaggggc tgatcaatgt tgtaaaaaat
     6541 actatttgtc ctgccaattc atcattgcaa tcatgctgct gtgccaaagt atagcttgcc
     6601 acagccgtag ggggagctat catgcttaat actgctccta attcaattcc tctgtatccg
     6661 aagaatatgg taatcaatgc tgcaaatgaa gggatgaaaa ataattttac tgctgctata
     6721 agaattaact tttttctgtt tttccaaata ttgtttattt ctatcactcc gccgagaatc
     6781 attaatgata tgggtgttac cgtttttgag atatcagcaa ttgttttatt aagggcggag
     6841 ggaagtttta tttgaagaac agatgctgca attccaagca aggagccgat tactaaagga
     6901 tttgttaaaa tgcttataat agtatcttta taattatgcc tttcatccgt aaataaatcc
     6961 agaataataa cagccaagaa attataaatt ggcactatta ttgcgataag ggttgataga
     7021 acaaccaatc cttcatctcc agaaatattg gccataagag gtattccaaa aaacacaaat
     7081 tgccctctaa acagaccctg aattaaaact cctctgtttc tgttatcttt ttcgtacttg
     7141 gggattacta tcaataatat tccaataaca gccaaagcta aaataacaaa aaaaataata
     7201 aattttaagt caatttcccc tgataccttg cttgtatata tgttgttaaa aagaagaacc
     7261 ggtaatagta cacaatatgt aattttattt aatttcctta aaaagtcctc atcaaatata
     7321 ttaattcttc gaagaaagga acctataaac atcaaaaaaa ccaatggtaa aacaacattt
     7381 aaagaaaata taaaattatc catcttttga cctctttaaa ataatcctgc tgctttaaaa
     7441 atgtatacaa acaagaaaat agtgaatggg ctgattaatg tagttaaaaa tactatttgt
     7501 cctgccaatt cataattgca gttgtgctgc tgtgccaagg catagcttgt gacagccgta
     7561 ggaggtgctg tcatgcttaa tattacacct aattccactc ctctgtatcc gtaggaaatt
     7621 gcaatcaagg ttgcaattgc aggaaaaata attagttttc ccgctacaac aaaaactaag
     7681 ggtttaatat tcttccatat attgtttatc tctatttctc cgccaagggt catcaatgct
     7741 atgggcgttg tagtttttgc aatatcggca attgtttttt ccattgccaa aggaagtttt
     7801 atatgaagag tatatactgc aataccgagc agggagccga ttattaaagg atttgataaa
     7861 atgcttatta aagttttctt ataattttgt ctttcatcag taaataaatc caaaattata
     7921 acagtcaaga aaatataagt gggtgatatt attgctatca gcattgttac aatagccaac
     7981 ccttcatttc caaaaatatt gctgattagt gctgttgcaa acaaggtggt attgccgcga
     8041 taaaggcctt gaattataac tcctctgttc ttattatctt tttcatattt agggattatt
     8101 acaatcaata atccaacaac agctatagat attataacaa caaatatgat aaattttgaa
     8161 tctatttctt cagatatctt actggaatat atgttgttga aaagaaaaaa cgggaataat
     8221 actttaaaaa taaacttatt tgcttttcct aaaaaatctt catcaaaaac attgattttt
     8281 cgaaggaaag cacccaaaag catcaaaaaa accaatggta aaactgcatt taaagaaaat
     8341 attaaatttt ccattataag tcctttcata ttctgtaata ataattaatc tgtgtagtca
     8401 tgctcttgac attatatatc aatcattttc aatttttgaa tacagcatgt cctattaatg
     8461 ttcatataca tgacttttca gtctattata attatactct caaaacaatt ctaaaacaat
     8521 agttttaatt gcataactat tttttaaaat atataatagc attataagga gcaatatccg
     8581 taaacagctt ttaaagaaat gcatgttctt gttgtcccgg attatcatgt aaaagtatta
     8641 taggaggcag aaatggaaaa tttaaaacta aaggattttt tggattatac ttttttatcc
     8701 ggacttgaat tgtcaccgga taaaaattac ggtgcttttg tggtacatac atcagatttt
     8761 gaggataaca agtatttgtc caatatatgg ttgtataatt tgaccacctc ggaatataaa
     8821 aagcttacaa atatgaatga agaaaagagt tttatttggc ttgacaataa tactattttg
     8881 ttttcttcat tgagagatga aaaattgaaa aaaagaattc aggacggcga gcattggact
     8941 gtattctatg ccattgatat ccaaggcggg gaagcttatg aatatatgcg tattcccatg
     9001 aaggtaaaca atattaaaaa aattgccgac gagaaatttc tattaacagc aatttatgac
     9061 cattacggca ttaaccttca ttctcgcaaa ggagaagaaa agactaaggc aattgaatta
     9121 ataaaagaaa acaaggacta tgaaatattg gatgaaatac cgttttggtc aaacggcgaa
     9181 ggatttataa acaagaaaag aaaccgtcta tatctgtttg acaaaggaac aaaggatatt
     9241 gtaccaattt caaatcaata tgaaaatata aaagtaacaa gcgtaaaaga cggtaaagct
     9301 ttatatgttt cttcaattta caccaataaa atggaattga caacaggatt atacatgtac
     9361 gatttatata gcagtaaagc tctttgcctt gttgaggatt tagaatataa tatagagttt
     9421 gccgaatttg taggggatga cataatagtt gcagctacta ccatggataa atacgggata
     9481 aatgaaaact tcaattttta taagctacaa gatggggaat tggtacttct cagcaaacac
     9541 gatttcggtt tgaacaattc ggttaactcg gactgcagat atggagaagg agctgcaaga
     9601 agagtattcg ataactgcct gtacttcata tccacagaaa ataaaagttc ctttataaag
     9661 aagctttcat tggatggaag tattgaaaaa ttaacggaag ataatggatc agtggactgc
     9721 ttcgacatat gcagccaagg tattatcttc attgggctca ggggcagcag gctgcaggaa
     9781 atttatacgt tacaaaaaat aaaagaaact caaagaacta aatttaatga aaagatagtg
     9841 gaaataaaga cgattgtaaa acctgaaagt attaattttc aaaacgatgg agttaccatt
     9901 gaaggatttg tgttaaaacc tgttaatttt gatgaaaaga agtgctatcc gggaatctta
     9961 aacatacacg gaggtccaaa aacagtatat ggtgaagttt tctatcatga aatgcagtat
    10021 tgggcaaacg aaggatattt tgtatttttc tgtaatccaa ggggcagtga cggcaggggt
    10081 gatgaatttg cagatataag aggtaaatac ggtaccattg actatgatga ccttatgaaa
    10141 tttacagata aagttcttga aaaatatcca cagatagatt gcacaagaat tggtgtaaca
    10201 ggcggctcct atggaggctt tatgacaaac tggattattg gacacactga cagatttaaa
    10261 tgtgctgttt cacaaaggag tatttcaaac tggatttcta aattcggcac tacggacata
    10321 gggtattatt tcaatacaga tcaaaacttg tcttctcctt gggataatgt tgaaaaaatg
    10381 tggtttcatt caccgcttaa atatgcaaat caagctgtta ctcccacatt attcattcat
    10441 gcggaggaag attacaggtg ctggctggct gaaggattac aaatgtttac tgcccttaag
    10501 tatcacggtg tcgatgcaag gctttgcatg ttcaggggag aaaatcacga tctttcaagg
    10561 agcggtaaac cccgccacag ggtgaggaga ttggaagaaa taactaattg gtttgaaaaa
    10621 tatttaagat gagtgaagga tctcatgcta aaagcttccc aatataggaa gctttttaat
    10681 gtactatcaa taacggggac caagtgtcgc ccttaattta gggacattcc tcatgcacaa
    10741 agggactata ccccaatcag aggtgtgtcc ccataccatt tagcagcggt caccgattcc
    10801 gccaactcta tcgcagctgc agaatagtat taccaacaat aagaagaaga ataataagct
    10861 gtcattgtcg ccaaagccgc cgattcctcc taccaatcca tcgtctccgc agaagcatgc
    10921 aagaactact aaaagcaaga agaagaataa taaactatct ccgctgtctc ccaatatatt
    10981 gttaaacata tagaacctcc taaaaataat tttaatatat attattcgtt agactgctaa
    11041 ttgttacttt attactcttc atttaatgat tttagataaa aaattatttt gtcgataaaa
    11101 ctgtctttcg tgcaataatt gtggcattca tgtttaatta aatttctatt gctgcatttg
    11161 cctctgtatt tatcatagct gtatttgaca ttttgcatat tgtccattat gtcaaataag
    11221 tattgtttcg aatgttcatc ctcacaaaat ttgtttgtga gtctgtttaa tatatatatt
    11281 aattcgatta tgtcgtacat tctttctatc atattatcgt cacattttct cttgtccact
    11341 ttcatcactc cattcattat attatatgaa ttttttgaag tgctgatagt agaattgaaa
    11401 catttatttc attattcctt tgattttatc aagttttatt gtataataag ttaaacagat
    11461 tattcacata cgttcagaat tatcacgtat aatccacaca aaaataattg aatacatttt
    11521 gtcatcctga gtgtactgcc ccttttacat aaattatata cgaggtacta atatgagagc
    11581 attttttaat atagtaataa taggacttat aggtttgtta tcatccacat atttgttcaa
    11641 tttgtcacct gttgactata aaggaacaat tgaaattgaa actcctgttg aagaaagtac
    11701 tttaagttta gaagtcgaag aagacaaaat actaaatcct gaatcaataa caataaagca
    11761 tacagcctct aaggaagagg taattaaaat tgtcagcaac agcattttta atgtagacat
    11821 atacaaggat aataaattgg ttaaaagtaa tattgataat ttagagccgg tttctccttc
    11881 aattgatgca accatttcag ttaataagga taatccaatc attactgccg taaatatagc
    11941 tcaaaaaaac ttaggactgg aagatggagt atataaattt gtatttaatt caaatataat
    12001 agcagatata gataaggctt ccgtatcagt aacagttacc tatgatacaa ccggtaatta
    12061 ttatcctgcg gttaatactg cccctgcggg tactaaggga ctcaccctgt acttcccaac
    12121 aaaaaatgca gatactttaa ttcctgttac gaggtttgta gtagaggata aatcaattac
    12181 aagaatggca attgagcagc tgcaaaacgg tcctttaagc aaagagctta tatcagtaat
    12241 caaggatgtt acaaacacaa cttacaataa cggtaatgtg gtaattgacc tgccaagttc
    12301 atatactgca tataataccg gcagcacggg agcagtcctg gcctacgaat catttgtaaa
    12361 aacaatattt gccgttgaca gatattggcc tattcacagc gtcacattta ctgttgacag
    12421 aaaaaatgta gacacttact tccatgggat gtcaagagag gcaataaatt atcttcctaa
    12481 tattaagaga aattaccttc tgtatctggc tcataaggca gacaacagat attatttatt
    12541 tgagtatcag ctatccccag aggaaatagg tattgctgaa aatgattcaa tagaaatgaa
    12601 ggcgcgaaaa atatttgatg catattcaaa tacagacatc gaatacggca ttagtccggt
    12661 acctaagact gtaacgctaa acaatgtatc tctgcaggga agaacactta ttcttgattt
    12721 taataaggat tttttgaatg tttatgagga taaaaatgat ttaagacata tgatgataga
    12781 atcctttttg tatactttca ccacaatacc gggtgttgac agcataaaaa ttactgccga
    12841 aaataaagaa ataactgatt ttattgatgt gctggataca acaaaaatat tatatcctcc
    12901 ggaatttatt aatccggaag cagtagaaga gaaagaagag taatcaaaaa aaaaagtaag
    12961 ctatgtcatt ctgaagcctc agaatgacat gcagcttatt aattgcaata acaggctttg
    13021 cctaatggtt attcatacaa tcgcccttgt ctttttaaca atgaagcata caagtttggg
    13081 gacattcctt ctgactaacc tcatgacttt cacctaagga ggtgtgtccc cacaccttta
    13141 acaggtgtat gtttgaaaac ttccaaaaat gctgctgata ctattatgcc tacaacagac
    13201 tgagctgtat tgccgggtat ttcaccgatg gttgttaaga agttacctac catcaaggaa
    13261 cctaccacat aatatgaaag tacctgccaa atgcttcccg ttattgcacc ggcaacccaa
    13321 ttatgcttaa gggatggaag cttttcaatc atataaccca taattaaagc cataacacct
    13381 ttaattataa aagttggcaa tgcataaata ctgtacccgc cgattatatc agcaagagcc
    13441 gcgcctattg caccagccca tgcaccattt ttcttgccga ttaatattac cgcgagaaat
    13501 ataaatccgt ctcctatgtg agcgtaccct cctgatacag gcagcggtac ttttattata
    13561 aaggttgcaa cacaaactaa ggcagcaaac aaagctgtgt ataccatatc tttaactttc
    13621 atgtcatcca ttattatcac catcccataa tttacttccg gttaatctaa ttatatatca
    13681 atctgtactc tttaaaagat acagattgca aatttattcg gatgacagat taatatttaa
    13741 aataaaagac tcttcatgac caaaatgact acctgtcatt ttgagggcgc agcccgaaga
    13801 atctcattaa aaagctgtgc acccttcttc gacaatacat aacaagcgtt acttcagcag
    13861 ttagctcaaa cccggcaccc tcacgtcaca caaaagtaac tacttactgc tgcttccttc
    13921 cggacctgac agggttcata ggtttttgtt gcgcgagacc gagttatcaa caccacttgc
    13981 taaaggcagg cctcactatg caagcctcgg agagggaatt ccaccttgct gcagcggatt
    14041 gcaagtacag ggcaccgcta cctccccatg tagcacagca caaaaatgat aacataatta
    14101 aaatcatgtg tcaatgttat aatttgcctc
//
LOCUS       DAOSHP010000043        13831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3680_length_13831_cov_8.304805, whole genome shotgun sequence.
ACCESSION   DAOSHP010000043 DAOSHP010000000
VERSION     DAOSHP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13831
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_13831_cov_8.304805"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..723
                     /locus_tag="PLB33_05635"
     CDS             151..723
                     /locus_tag="PLB33_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63037.1"
                     /translation="MNRNNSSSMVISALLCAIGIIIPIISPLKITLEPASFTLASHVA
                     IFIAMFISPASALFVAAGTAAGFMIAGFPIVIVLRAATHIVFATLGAIYLKKNPNALN
                     SFKSSQIFSLAIGVLHGISEVLIVMPFYFGNNMSSAYYVKGFIVSVILLVGVGTVVHS
                     MVDFYLAKAIWKPVTKAVKIPIDVSVKYNA"
     gene            857..1936
                     /locus_tag="PLB33_05640"
     CDS             857..1936
                     /locus_tag="PLB33_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63038.1"
                     /translation="MELITASKNNRRHFLKFYIKQYLNNDLKRDTLSGMLKNLLYGKS
                     KLCGSVDMEPVMVVENNEIIMVSLLAHAHRMPEYLQICFFESEEYNMDAFNLILDRAK
                     DMAKEKKAAKLTGSLNIHVNYGLGFLASDFDKKQSFGAPHNPEFYNYYFEKSGFEIIN
                     MVSYKKDMTKSPDIINNNVREKLKEIYTVRKADFKNFKKDIEIYTKINNEAFSNHLFY
                     YTRNTEEDYELFKELKYLLREENLIFVEKQGVPVGFMLWYPDFNEIIEKNETVGIKTV
                     LKNKLFPHRIKTFKIVEIGVIPEERNKGAILALFDYCNKCVKGRYDTMESSWILSDNL
                     KSKSFGIKWADGEFKHYKAYVKEII"
     gene            1933..2877
                     /locus_tag="PLB33_05645"
     CDS             1933..2877
                     /locus_tag="PLB33_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63039.1"
                     /translation="MIIYKADKLKKIKIFDLNEEYIRLLSLLEKINPCNQFYEATKDC
                     LIISYELKLNLLNFINLLPLKAKAQIIGLPISVSQRGYYGDLNKTIEIIKKRKGLKII
                     LNADEEIIKNARTLSTFVFENNFETFDDYLNKLRSSYRRRINKALEYRSNLIINKIQN
                     NEFSEKHYNLYLSIMNRTDNPLETLPIEFFKNYDSEIYEFLNKGTLNVIGFVQLKEFD
                     GRLCFLFGGFNKDDVKKYDLYYNMLLKIIEAGIEKKAHIIEFGQTAEESKLKIGCKEV
                     YKYLYVHHSNSILNFIIQKLLPIMSYRPYRTVHHVFKE"
     gene            2882..4150
                     /locus_tag="PLB33_05650"
     CDS             2882..4150
                     /locus_tag="PLB33_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63040.1"
                     /translation="MIVFLEKVRVKTIFSIIDPVKVEPLELCYLKSVLNNMNIESYIV
                     DELFDFSPPKDITPDIVVLTGYNTAENEIIRMSEVYKNKYPPVKIIAGGVHVQENAEN
                     FRVNTIDYVFHSPSLNTFRLLINKIINNDGKPLNRGVDTNYKGDWHLGEKESIYEIEN
                     IKPDRDFFYKAGTKLKYLDKKNVSLIKGSIGCPYNCSFCYCKLLNNKHFITADYEKMI
                     EEMESINADYYWVVDDVLFSTRNDALKFIEIIEKRNSNLNIIGYLRADFICREKDLLK
                     RLRNAGIREVITGFEATGNKELNEYKKTTDAADYPLAISLLKENNIELTALFMVQPSY
                     VLKEFKNLKKFIKTNKIDLYTISIMTPLKKTKLYEETEELLIDKRPDKYDFLHLVIKP
                     KLPKFIFYTLFYSLHIRLLKSKRVRKFLFN"
     gene            4162..4779
                     /locus_tag="PLB33_05655"
     CDS             4162..4779
                     /locus_tag="PLB33_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOG63041.1"
                     /translation="MFNIWNFWADKYDKLWVQKYSLKPTRNYIVKALSKYIKNDGIKV
                     LDLGCGTGELIFELTNKFNNLEVTGIDFSEKMIEISKKRNPSAKHLQMDASELYILDS
                     KYDIIVCTHSMPYYKEPKNVFKHLYNLLSDEGKLLIGFASGDSIYDKFILGFVKLTTG
                     KANYPSDNEFRAMIFPFFEVENLKIIRKKFFMPRIAVYTLKKVKL"
     gene            4776..6137
                     /locus_tag="PLB33_05660"
     CDS             4776..6137
                     /locus_tag="PLB33_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG63042.1"
                     /translation="MKVLLVRPRPHKETIGLQHVMICEPLELEYLISNIPDDIKGETD
                     AEIIDMILEKGSFEEVVYKKKPAFIVFTGYITHVGIIKQMAETAKKIIPSVKTGVGGV
                     HAEVVPEDFESEYIDFVYYKNGIDNFNITLRGLINKKSINEIKEEINIEDKKVIFDYK
                     YPDRNSVKKYRKKYYYMFHNPCALIKTSFGCPYNCSFCFCKEITQGQYFTREMDDIIK
                     ELSSIEEEEVYIVDDDFLYDRNKLESFIAGLKLNNIKKKFLVYGRADFIAQNKDIIAK
                     LKEVGLQAVIVGIESVRAGDLKDYKKGTTVEINEKCIQILQELDIELYATLIIPLDFK
                     KEDFSELVSWLRKLNIKFVNLQPLTPLPGTEIFESYKGSLLADRQRFEVWDMAHVVLK
                     PRYMSIRAFYAEILKAYYKVVMRPKHLGYLIKKYGLKANFKMLAGSTFVSFQYLQKII
                     RSN"
     gene            6137..7459
                     /locus_tag="PLB33_05665"
     CDS             6137..7459
                     /locus_tag="PLB33_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOG63043.1"
                     /translation="MKKILLIQASPYDKNHKPIKKNKLYFVGLAMPLLAAMMPEDWEA
                     EIVLEVIEDIPFDTDAELIGISTMGHGVIRSIDIARKFREMGKTVILGGYMASIMADE
                     ALKHCDSVVVGDAELVWNELLKDYDEGRLKKIYKDNLENGFFSTPMPRFDLIINKRIG
                     NFLPVQAGRGCPNTCSFCSVACLYKGHYIKKPLEEVVRDIKQVKELGFKKFLLLDDNI
                     FSDREYLDKLLIEIKKLNMQWMSQCDIRIGRENELLKKLADSGCTTLSFGLESISKES
                     LKGMNKSWANPLEYTELIQNIHSHGIDVSTEMVVGGEGDTLQSIRDTKRFIENNKITV
                     PRFYILTPIPGTKFFEDMTKAGRIIKEDIYSFDGTEAVHRPKNMSPEELTNAYWELYD
                     SLFTFSSILKRNILRKEFLINPSKYLFYTIVNLFYRYQIKNRITPNIF"
     gene            7502..8860
                     /locus_tag="PLB33_05670"
     CDS             7502..8860
                     /locus_tag="PLB33_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971736.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOG63044.1"
                     /translation="MRILLVRPPRIKQAITLSDFMFSEPLGLEMIYGVLKEDHEVEIF
                     DMMIETDSLAVKIVEFKPELVGITSLCIDVIKVLKLCKETKEINSNIITAVGGTQAYL
                     NPEGFYNENVDYIFEYTTRDNINILFDSLKKEEKILIDGVRTRSLSYKSTGQKGRNEY
                     MLPDRSSTNKYRNEYSYFGYKPAAIMEFGTGCKKVCDFCLRWRIEGAEEKLIDIELTK
                     NDLRNIKEPTVMFIDNDFLCSEEKLNSFISIVKNLNFKKNYIMYGSVKGVIRYEKYLK
                     ELHEIGLKALLVGYETFNDDELLKYRKSSSTDDNLKASEILRKNKIDVWASFMAHPDW
                     NKNDFISLRKYIKKLRPQITSISPLTPFPNMPMYEEFEDRLLYQKEDFEKWSFGQVMI
                     KPSKISLREYYFELLKTNLYVNIFINKNTEMIKKFGIINIFRILKGSLKAVKKYVSLM
                     YE"
     gene            8945..9118
                     /locus_tag="PLB33_05675"
     CDS             8945..9118
                     /locus_tag="PLB33_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63045.1"
                     /translation="MDKNFEEIKEKFINASLEEKIKIYTTTQGLTVEQFKELLAYFPI
                     KYLDKLEEAVNSN"
     gene            9200..10096
                     /locus_tag="PLB33_05680"
     CDS             9200..10096
                     /locus_tag="PLB33_05680"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOG63046.1"
                     /translation="MIKDNKLKKTYIKEALGNRNYLSYRTDLILLKILVSFAVFIATY
                     FIYLDLILSLLIASLVFFIFTLINKINVNKKNEKGREILIKKVKEEYFSSKIEEINQS
                     DFELLIKLFFKNEGYNNFIKKGRNLYLAEKDGYITCIKIFKLYGGIEVEKLDVRSLLT
                     FMGNSNIRNGFLVTTSGLSKDAAELIEKFKDKFEITIIDIEGMYNLADKYNMLPDESF
                     YYKKLSEEKTVTKKDFKENILNIKKIYLYIAASVFFYMTSVWMPENKLVIYISYYFII
                     LTTINILYFLVNKYNIKKVNRG"
     gene            10200..10952
                     /locus_tag="PLB33_05685"
     CDS             10200..10952
                     /locus_tag="PLB33_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HOG63047.1"
                     /translation="MGNLMLERQLNHRTIREFKDGRIPEELFNKLIEVARRTPTSNGM
                     QASSIIRVTDNQKRKEIADVCKQEYVARAPELLILIADQYRNYRIADEKGGYEPSARD
                     MDRFFQGFTDACLTAQNIVNAAEALDLGVVYLGSILNDAEKIIEILELPELTMPVLGL
                     GIGYPNQNPQLKPRMSMDLRLFENTYKKFDDYLEEIKEYDIEMQTYYDLREANKRVDS
                     FSNQVVTKLKNLIPKRSDMLKVMKKQGFDLNI"
     gene            11108..11686
                     /locus_tag="PLB33_05690"
     CDS             11108..11686
                     /locus_tag="PLB33_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOG63048.1"
                     /translation="MRFKVTVGLSNKHVHLSKEHIDILFGEGYELTPIKDLTQPGQFA
                     CDEKVDLVGPKRTIKGVRILGPARKETQVELSFSDGFTLGITDLPVRDSGKTEGTPGL
                     KLVGPKGEVEIERGAIAAARHLHMHTTDAEKYGLKDKDIVSVKVDGQRGLVFDNVLVR
                     VNEAYALDMHVDIEEGNAAGLKNGDLVEVIVE"
     gene            11975..13771
                     /locus_tag="PLB33_05695"
     CDS             11975..13771
                     /locus_tag="PLB33_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOG63049.1"
                     /translation="MDFLPVNMEDMKKRGWDECDFVFVTGDAYVDHSSFAAAVLSRTL
                     EKFGYKVGIIPQPDWKELSSFKVLGKPRLAFLVNSGNIDTMVNHYTTFKKRRHQDAYS
                     PGGKAGLRPDRAVTVYSNRIREAYKDIPVIIGGIEASLRRFSHYDYWSDSVKRSVLLD
                     SGADLLVYGMGEKVLIEIAEALESGIPVEEITFINQTVYKTKDRERPYNPIFLPDYNE
                     VVSSKKKYGESFKIQYENTDSKSARTLVEPYNTVYVVQNPPMEILSQQELDDIYDMDY
                     MRDYHPMYKNLGVPAFNEVKFSITSNRGCFGGCSFCSLNFHQGRIIQARSHESILNEV
                     EKMKKDKDFKGYIHDVGGPTANFRIKACKKQEKYGACKNKECLTPQKCENLQIDHKDY
                     IELLKKLRSIEGIKKVFIRSGIRYDYAIYDNNDDFIEELCKYHVSGQLRTAPEHVSES
                     VLKLMGKPSIKIYNKFVEKFNDVNKKIKKEQYIVPYFISSHPGSRLKDAIELAEYIRD
                     MGHIPEQVQDFYPTPGTLSTCMYYTGFNPLTNKEVYVPKDIEEKKMQRALLQYSKKEN
                     YELVLKALKKENRYDLIGYGPKALIKPRQKKH"
BASE COUNT         5211 a         1801 c         2632 g         4187 t
ORIGIN      
        1 tatgttagag cagaaatagc aatcataaga agttatcttg ataatattat gcttataata
       61 tcttgacaaa attaaaaaac tattctataa tcctatcaac aggtgtcaag acatatgtca
      121 agacaataat gcattggaaa ggatagaata atgaatagga ataatagtag ctcaatggta
      181 atatctgctc ttttgtgcgc tatcggaata ataataccga taatatcacc gcttaaaata
      241 acattggagc cggcatcatt tacactggcc agccatgtgg caatttttat tgcaatgttt
      301 atttcacctg cttctgcatt atttgttgca gcaggtacag cagcaggttt tatgattgca
      361 ggatttccaa tagtaatagt attacgtgct gcaacacata ttgtttttgc aactttagga
      421 gcaatttatt tgaaaaagaa tccgaatgcc ttaaattctt ttaaatcttc acaaattttt
      481 tcgttggcaa tcggagtgct gcacggaata agcgaagtgt taatagtaat gcctttttat
      541 tttggaaata atatgagctc tgcttactac gtcaaaggat ttattgtttc cgtaattttg
      601 ttggtgggag ttggaactgt cgtacacagc atggtagact tttacttggc aaaggcaatt
      661 tggaagcccg ttacaaaggc agttaaaatc ccgattgatg taagtgtgaa atataacgca
      721 taggattact cagactgccc cttcagaatg gttcattggg aatatattgt catctcaaga
      781 ctgttaaaca gtctttttta ttttgtaata ctttataagc tatgctataa taactataaa
      841 aatttatgga gcaatcatgg aattaattac tgcaagcaag aataacagaa ggcatttttt
      901 aaagttttat ataaaacaat atttgaataa tgatttaaaa agagatacat tgtcaggcat
      961 gctgaaaaac ttattgtatg gaaaatccaa gctgtgcggc tccgtagata tggagcctgt
     1021 tatggtagtg gaaaacaatg aaattataat ggtctctctt cttgcacatg cgcatagaat
     1081 gcctgaatat ctgcaaatat gcttctttga gtctgaagaa tacaatatgg atgcttttaa
     1141 tctaatttta gacagggcta aagatatggc aaaagaaaaa aaagctgcca aactaacagg
     1201 ctctttgaat atacatgtta actacggact tggtttttta gcaagcgact ttgataaaaa
     1261 acaaagtttc ggggcaccgc acaatcctga attttataat tattattttg aaaaaagcgg
     1321 ttttgaaata attaacatgg tttcttataa aaaagatatg acaaaatctc ctgatataat
     1381 aaataataat gtcagagaaa aacttaaaga aatttacaca gtgaggaagg cagattttaa
     1441 aaattttaaa aaggatattg aaatatatac aaaaattaac aatgaagcat tcagcaatca
     1501 tttattttat tacacaagga atacagaaga agattatgag ctgttcaagg agctgaagta
     1561 tttattgagg gaggaaaatt taatatttgt tgaaaaacaa ggagtgccgg tcggtttcat
     1621 gctttggtat cctgatttta acgaaataat tgaaaagaat gaaactgtcg gtattaagac
     1681 ggtgcttaaa aataaattat ttcctcatag aataaaaaca tttaaaatag ttgaaatagg
     1741 cgttatacct gaagaaagaa acaagggtgc aatacttgca ttatttgatt attgcaataa
     1801 atgcgttaag ggaagatatg atactatgga atcaagctgg atactttcag ataatctaaa
     1861 gtcaaaaagt tttggcataa aatgggctga tggtgaattc aaacattata aagcatacgt
     1921 taaggaaatt atatgataat atataaagcg gataaactta aaaaaataaa aatatttgat
     1981 ttaaatgaag aatatataag actcttgtcc ttgttagaaa aaataaaccc ctgcaatcag
     2041 ttttatgaag caaccaagga ctgtttaata ataagctatg agctaaagtt gaatttatta
     2101 aattttatta atcttttgcc attaaaagca aaagctcaaa ttattgggct tcccatatcg
     2161 gtatctcaaa gaggatacta tggtgattta aacaaaacaa ttgaaattat caaaaaaagg
     2221 aaaggtttaa aaataatttt aaatgctgat gaagaaatta ttaaaaatgc aaggacttta
     2281 tcaacctttg tgtttgaaaa taactttgaa acatttgacg attatttaaa taagctcaga
     2341 agttcttaca gaagacgtat aaataaagct cttgaataca ggagcaattt aataatcaat
     2401 aaaattcaaa acaatgaatt cagtgaaaaa cattacaatt tatatttaag cattatgaac
     2461 agaaccgata atcctttgga aactctaccg attgaatttt ttaaaaatta tgattctgaa
     2521 atttatgagt ttttgaataa aggcacccta aatgttattg gatttgtaca actgaaggaa
     2581 tttgacggca ggctgtgctt tttatttggg ggatttaaca aagacgatgt aaaaaaatat
     2641 gatttatatt acaatatgct tttaaagatt atcgaagcag gcattgaaaa gaaagctcac
     2701 attattgaat ttgggcagac agcagaagaa agtaaattaa aaatcggctg caaggaagta
     2761 tataaatatc tttatgttca tcacagcaat tctattttaa attttataat acaaaaattg
     2821 cttcctatta tgtcatacag accatacagg acagtccatc atgtttttaa ggaatgaaac
     2881 tatgatagtt tttttagaaa aagtaagggt taaaaccata ttttcaatca ttgaccctgt
     2941 aaaggttgag cccttggaat tatgctattt gaagtcggtt ttaaataata tgaatattga
     3001 aagctatatt gtagacgaac tgtttgattt tagtcctcca aaagacatta ctcctgatat
     3061 tgtagtgctt acgggttaca atacggcaga aaatgaaatt ataaggatgt ctgaggtata
     3121 caagaataaa tatccaccgg taaaaataat agcaggggga gttcatgtgc aggaaaatgc
     3181 agaaaatttc agggtaaata caatagacta tgtttttcat tcaccaagct taaatacatt
     3241 taggctcttg attaataaaa tcatcaataa tgacggtaaa cctttaaacc gtggtgttga
     3301 tacaaattat aagggagact ggcacctggg agaaaaagag agtatttacg aaattgagaa
     3361 tattaaaccg gacagggatt ttttttataa agccgggaca aaactaaaat atttagataa
     3421 aaaaaatgtg tcccttataa aaggcagcat agggtgtccg tacaattgct cattttgtta
     3481 ctgcaaactt cttaacaaca agcatttcat aactgcagat tatgaaaaaa tgattgaaga
     3541 aatggaaagt attaatgccg attactattg ggtagttgat gatgtattgt tttctacaag
     3601 aaatgatgct ttaaaattta ttgaaattat tgaaaaaaga aattccaact taaatatcat
     3661 aggatattta agggcagatt tcatatgcag ggaaaaggac ttgttaaaga ggcttagaaa
     3721 tgcaggcatt cgagaggtta ttaccggctt tgaagcaaca ggaaacaagg agctgaatga
     3781 atacaaaaaa acaaccgatg ctgcggacta tcccttggca atttccttat taaaagaaaa
     3841 caatattgaa ttaacagccc tttttatggt gcagccaagc tatgtcctaa aagaatttaa
     3901 gaatttaaaa aagtttataa aaacaaataa aatagatttg tacaccattt caattatgac
     3961 tcctctaaag aaaacaaagc tttatgaaga aacggaagag ttactgattg ataagagacc
     4021 tgataagtat gattttttgc acttggtaat aaaaccgaaa ctgccgaaat ttatttttta
     4081 tacattgttt tacagccttc acataagact tctaaaatca aaaagagtaa gaaaatttct
     4141 gttcaattaa gaggagagca tatgtttaat atatggaatt tttgggctga taaatatgat
     4201 aagctgtggg tgcaaaaata ttctttaaaa cccacgagaa attatatagt taaagctctt
     4261 tcaaaatata taaaaaatga tggaataaaa gtacttgatt tagggtgcgg cactggagaa
     4321 ttaatttttg aattaacaaa taaatttaat aatcttgaag ttacgggaat tgatttttcg
     4381 gaaaaaatga ttgaaatatc aaaaaaaaga aatccctcag ccaaacattt acaaatggat
     4441 gcatctgagt tatatatttt ggatagcaag tatgacataa tagtgtgcac ccattcaatg
     4501 ccttactaca aagagccaaa gaatgttttc aaacacctgt ataatttatt aagcgatgaa
     4561 ggaaaattac ttattgggtt tgcaagcggt gacagcatat atgacaaatt catattaggc
     4621 tttgttaagc ttaccaccgg caaggccaat tatccctcgg ataatgaatt cagagcaatg
     4681 atatttccct ttttcgaagt ggaaaattta aaaataatca ggaagaaatt ttttatgcct
     4741 cgaatagcag tctacactct taagaaggtg aaattatgaa agtattactc gtaagaccaa
     4801 gacctcataa agaaacgata ggtttgcagc atgtaatgat atgcgagcct ttagagcttg
     4861 aatatcttat ttcgaatatt cctgatgata tcaaaggtga aacagatgct gaaattatag
     4921 atatgatact tgaaaaagga agttttgaag aggttgtata taagaagaaa cctgcattca
     4981 tagttttcac cggctatata acccatgtcg ggattataaa gcaaatggca gaaactgcga
     5041 aaaaaattat accttcggtt aagacggggg tagggggagt tcatgcagaa gttgtacctg
     5101 aggactttga atcggaatat atagattttg tttattataa aaacggaatt gataatttta
     5161 atataacatt aagaggttta ataaataaaa aaagtattaa tgaaataaaa gaagaaataa
     5221 atattgaaga taaaaaagtc atatttgatt acaaatatcc tgacagaaat tcagtaaaaa
     5281 aatatagaaa aaaatactac tatatgtttc acaacccttg cgccttgatt aaaacttcct
     5341 ttggctgccc atacaactgc tcgttttgtt tttgtaaaga aataacccaa ggtcaatatt
     5401 ttacccgtga aatggacgat attataaaag aattatcctc aattgaagaa gaggaagtat
     5461 atatagtaga tgatgatttt ttatatgata gaaacaaact tgaaagtttc attgcaggac
     5521 taaagttaaa taatataaaa aagaaatttt tggtttacgg aagagctgat tttattgcac
     5581 aaaacaaaga tattatagca aaacttaagg aagtcggctt gcaggccgta atagtcggaa
     5641 tagaatcagt aagagcaggt gatttaaagg attacaagaa gggaacaacg gttgaaataa
     5701 acgaaaaatg cattcaaata ttacaggaat tagatattga actatatgca acattgataa
     5761 ttccccttga ttttaaaaaa gaagatttca gtgagttggt aagttggctc aggaaattaa
     5821 atataaaatt tgttaacctt cagccgctta cacctcttcc cggaactgaa atatttgagt
     5881 cttataaagg ctcactcttg gcagacagac agagatttga agtgtgggat atggcacatg
     5941 ttgttttaaa accaagatat atgagtatca gagcttttta tgcagaaatt ctaaaagcat
     6001 attacaaggt tgtaatgaga ccgaaacatt taggatattt aatcaagaaa tacggcttga
     6061 aagcaaattt taaaatgctt gcaggctcaa cctttgtaag ttttcagtat ttacaaaaaa
     6121 ttatcaggag taattaatga aaaaaatatt attgatacaa gcaagcccct atgacaaaaa
     6181 ccataaacct atcaagaaaa acaaattgta ttttgttgga cttgccatgc ctttactggc
     6241 agcaatgatg cctgaagact gggaagctga aattgtatta gaggtaattg aagatattcc
     6301 ttttgatacg gatgccgaat taatcggaat cagcaccatg ggacacgggg ttatccgctc
     6361 aattgatatc gcaaggaaat ttcgcgaaat gggaaagacc gtaatactgg gaggctacat
     6421 ggcaagcatc atggctgatg aagcattgaa gcactgcgac agtgttgtag tgggagatgc
     6481 agaattggtt tggaatgagc tattaaaaga ttacgatgaa ggaagactca agaaaatata
     6541 taaggataat ttggaaaacg gctttttttc aactccaatg cctcgatttg accttataat
     6601 aaataaaaga atcggtaatt ttctgcctgt tcaggcagga aggggctgtc ccaacacatg
     6661 cagcttctgt tctgttgcat gcctgtataa aggtcattat attaaaaagc ctcttgaaga
     6721 ggtagttaga gatattaagc aggtaaaaga gttaggattt aaaaagtttc ttttgttgga
     6781 tgacaatatt ttttctgaca gagaatattt ggacaaactg ttgattgaaa ttaaaaaatt
     6841 aaatatgcag tggatgagcc agtgtgatat tcgtataggc agagagaatg aactgttaaa
     6901 aaaactggct gacagcggat gtacaacatt aagttttgga cttgaaagta tttcaaagga
     6961 aagtttaaag gggatgaaca aatcctgggc aaatcctttg gaatatactg agcttataca
     7021 aaacatacat tctcacggta tagacgtttc aacagaaatg gttgtaggtg gcgagggaga
     7081 cacactgcaa tccataaggg atacaaaaag atttattgaa aataacaaaa tcactgtgcc
     7141 gcggttctat atattaactc ctattcccgg aactaaattt tttgaggata tgacgaaagc
     7201 aggaaggatt atcaaagaag acatttattc ctttgatggt acggaggcag tgcataggcc
     7261 taagaatatg agtcccgaag aattaacaaa tgcatactgg gaattgtatg attcattgtt
     7321 tacattcagc tcgattttaa aaaggaatat attaagaaaa gaatttttaa taaatccaag
     7381 caaatatttg ttttatacaa ttgtaaattt gttttacaga taccagataa aaaatagaat
     7441 tacgccgaat atattttaat gccctaaccc cattttaaaa cagcaaaaga taggagccac
     7501 aatgagaata ttattggtaa gaccaccgag aattaaacag gcaataacac tgagcgactt
     7561 tatgttttcc gagcccttgg gtttggaaat gatttacggt gttttaaaag aagatcatga
     7621 agttgaaatt ttcgacatga tgattgaaac tgacagccta gctgtaaaaa tagttgaatt
     7681 taaaccggaa ctggttggaa ttacatccct ttgcattgat gttatcaagg ttttaaaact
     7741 ttgcaaagaa acaaaagaaa taaattcaaa tataataacg gctgtggggg gaactcaagc
     7801 ctatttgaat ccggaaggct tttataatga aaatgtggac tatatttttg aatatacaac
     7861 aagagacaac attaatattc tttttgactc actaaaaaaa gaagaaaaaa tattgattga
     7921 cggagttagg acgaggtctt tgtcatataa atcaacagga caaaaaggaa gaaatgaata
     7981 tatgctgcct gacagaagtt caacgaataa gtacaggaat gaatattcat attttggcta
     8041 taaacctgct gcaataatgg aattcggcac aggctgtaaa aaggtatgcg atttttgtct
     8101 caggtggaga attgaaggtg cagaagaaaa actgatagat attgagttaa ccaaaaatga
     8161 tttaagaaat attaaagagc cgacagtaat gtttattgac aatgattttt tgtgcagcga
     8221 agaaaagctc aattcattta tcagtattgt aaaaaacctg aattttaaaa agaattatat
     8281 tatgtacggc tccgtaaaag gtgttatccg gtatgaaaaa tatttaaagg aattgcatga
     8341 aatagggttg aaagctcttc ttgtaggcta tgaaacattt aatgatgacg aattattaaa
     8401 atatcgcaag agctcatcga ctgacgataa tctaaaggct tcagaaatac tgagaaagaa
     8461 caaaattgat gtatgggctt ctttcatggc tcatccggac tggaataaga atgattttat
     8521 ttctttaaga aagtatatta aaaagcttag acctcagatt acttccataa gccccttgac
     8581 tccttttccc aatatgccca tgtatgaaga gtttgaagac agacttcttt atcaaaaaga
     8641 agattttgaa aagtggagtt ttggacaggt gatgataaaa ccttcaaaaa taagcttaag
     8701 ggaatattat tttgaattat tgaaaacaaa tttatatgtc aatatattta tcaataagaa
     8761 tactgaaatg attaaaaaat ttggaattat taatatattt agaattttaa agggctcttt
     8821 aaaggcagta aaaaagtatg tatcattaat gtatgaataa aaaaaataaa aaattttcct
     8881 ataatacatt ttgataattg tgaaataata taaaaataga gaaatttcaa aggaaaggtg
     8941 tattatggat aaaaattttg aagaaataaa agaaaaattt attaatgcaa gcttagaaga
     9001 aaaaattaaa atttatacta caacacaagg tcttacggta gaacaattta aagaattatt
     9061 ggcatatttc cccataaagt atttagataa gcttgaagaa gccgtaaata gcaattaaaa
     9121 tatggcgaat gtccctgtca attatccctg cagcgtataa attacctcaa tttggaaata
     9181 attccattga ggtgatttta tgataaagga caataaatta aagaaaactt atataaaaga
     9241 ggcattggga aacagaaatt acttaagcta tcgcacagat ttaatactgt taaagatact
     9301 tgtaagcttc gcagttttta tagcaacata ttttatatac ttggacctta tattatcttt
     9361 actgattgct tccttggtat tcttcatttt tactttaata aacaaaataa atgtgaacaa
     9421 aaagaatgaa aaaggcaggg aaattttaat taaaaaagtg aaagaggaat atttctcatc
     9481 aaaaattgaa gaaattaatc aaagtgattt tgaattgctg attaaattat ttttcaagaa
     9541 tgagggatac aataatttta ttaaaaaagg aagaaactta tatttggcgg aaaaggatgg
     9601 ttatataacc tgtataaaaa tatttaagct ttatggcggt attgaagtag aaaaacttga
     9661 tgtgagaagt ttgttaacct tcatgggaaa cagcaatata agaaatggtt ttttggttac
     9721 tacatccgga ttaagcaaag atgcagctga attaattgaa aagtttaaag acaaatttga
     9781 aataacaata attgatatag aaggtatgta taatttggca gacaaataca atatgcttcc
     9841 tgatgaatca ttttattata aaaaattaag cgaagaaaag actgttacaa aaaaagattt
     9901 caaggaaaat attctaaata taaaaaaaat atatttgtat attgcagctt cagtattctt
     9961 ttatatgacg tccgtctgga tgcctgaaaa caaattagtt atatatattt cttattattt
    10021 tattatattg acaacaataa atatattata ctttttagtt aataaatata atattaagaa
    10081 agtgaacaga ggttaagttg agtttagttt aggaacttaa aatacggtta ttagtttaga
    10141 tgattatata ttattttttg gggtatacca tatatataaa tataaaggag tagatgagta
    10201 tgggtaattt aatgttagaa agacagttaa atcacagaac aattcgtgaa tttaaggatg
    10261 gaagaatacc ggaagaattg tttaataaat taattgaagt agctagacgg actcctacat
    10321 caaacggaat gcaggcaagc tcgattattc gagtgaccga taatcaaaaa agaaaagaaa
    10381 ttgcagatgt ttgtaaacag gaatatgtgg caagagctcc agaactgtta attttaatag
    10441 cagaccagta cagaaattac agaattgcgg atgaaaaggg cggttatgag ccaagtgcca
    10501 gggatatgga tagattcttc cagggcttta cggatgcttg cctgactgcg caaaatatag
    10561 taaatgctgc tgaagcttta gatttgggag ttgtatattt gggaagcatt ttaaatgatg
    10621 cggaaaaaat tattgagatt ttagaactgc cggagcttac tatgccggtg cttggactcg
    10681 ggataggtta tccgaatcag aatccgcagc ttaaaccaag aatgagtatg gatttgcgct
    10741 tatttgaaaa tacatataag aaatttgatg attatttgga agaaattaaa gaatatgata
    10801 tagaaatgca gacctattat gatttaagag aagcaaacaa aagggtggac agcttcagca
    10861 atcaggttgt tacaaagcta aaaaatctta ttccgaaacg ctcggatatg ctgaaggtta
    10921 tgaaaaagca aggattcgac ttgaatatat aaggtctgtt ttattacacg aagacgattc
    10981 cctcggtagg cgggggaaaa aatatggtag ttatgagcaa taacaaatta aattaattaa
    11041 ttgttgtaat tttaactatt gtatattata atgcttttat atatacatta attttacgga
    11101 ggatgttatg agatttaaag ttacagtcgg tttatctaat aaacatgtac atttgagcaa
    11161 ggaacatatt gatatactgt tcggggaagg ctatgaactt acacctatta aagatttaac
    11221 tcaaccggga cagtttgcat gcgatgaaaa agttgatttg gtaggaccta aaagaacaat
    11281 caaaggtgtt aggattctgg gacctgcaag aaaagaaaca caggttgaat tatctttttc
    11341 tgatggattt acattaggta ttactgacct tcctgtgaga gattcaggaa aaacagaagg
    11401 tactccgggt cttaaacttg tgggaccgaa gggtgaagtt gagattgaaa gaggtgctat
    11461 tgctgcagca agacatttac atatgcatac aacagatgct gaaaaatacg gccttaaaga
    11521 taaagatata gtttctgtta aagtagatgg tcagaggggt ctggttttcg ataatgtatt
    11581 agtaagagta aatgaagcat atgcattaga catgcatgtt gacattgaag aaggaaatgc
    11641 tgccggactg aaaaacgggg atttagtaga agtaatagtg gaatagttta cataaaataa
    11701 tttatgtaaa ctccgaccgt taattctgtg caagtatttg gttctatata gtttgttgga
    11761 tacatgaaga tatataacaa ttttgctcta tgtaagtgaa ttggatacat ggtagatgtt
    11821 taaatgtctg aacagagtgt tattatttag ttctatatag ttattgaata catgttttta
    11881 tataaaagca gaggagtaat tcttctgctt ttattattgt tttaaatata atgcaggtgg
    11941 tataataaac acattaacat tatatggagg cattatggat tttttacccg ttaatatgga
    12001 agatatgaaa aaaagaggat gggatgagtg tgattttgtc tttgtgacag gtgatgcata
    12061 tgttgaccat tcttcatttg ccgctgctgt tttgtcaaga acattggaaa aattcggtta
    12121 caaggtcgga atcattccgc agcctgactg gaaagagtta tcttctttta aagtgttggg
    12181 aaagccgcgt ttagcctttc ttgttaattc agggaatata gatacaatgg ttaatcatta
    12241 tacgactttt aagaaaagaa gacatcaaga tgcttattct ccgggcggga aagcaggatt
    12301 aagacctgac agagctgtta cggtatattc aaaccgtata agagaagcat acaaggatat
    12361 tcccgtaata ataggcggta ttgaagcgag cttaagaaga tttagccatt atgactattg
    12421 gagcgacagc gttaaaaggt ctgttttatt ggattctggt gcggatttat tggtatacgg
    12481 catgggtgaa aaagttttga tagaaattgc ggaagcattg gaatccggta ttccggttga
    12541 agaaattaca tttataaacc aaaccgtata caagacaaag gacagagagc gtccctacaa
    12601 tccgatattt ttgcctgact ataatgaagt tgtatcttca aagaaaaagt atggtgaaag
    12661 ctttaaaata caatatgaaa ataccgactc caaaagtgca aggaccttag ttgagccgta
    12721 taatacagtc tatgtggtac aaaatccccc tatggaaatt ctatcacagc aagaattaga
    12781 tgatatttat gacatggatt atatgaggga ttaccatcct atgtataaaa acttgggagt
    12841 acctgcattt aatgaagtaa aattcagcat tacaagtaac agaggatgct tcggtggctg
    12901 cagcttttgc tcccttaatt tccatcaggg aagaataata caagcaagaa gtcatgagtc
    12961 catattaaat gaagttgaga agatgaagaa agacaaggac tttaaaggat atattcatga
    13021 tgtgggggga cctactgcta atttcagaat taaggcttgc aaaaaacagg aaaagtacgg
    13081 tgcgtgcaaa aataaagaat gtttaactcc gcaaaaatgc gaaaatttac agatcgacca
    13141 taaagattat atagaattat tgaagaagct gagaagtatc gaaggtatca aaaaagtttt
    13201 tattcgttca ggcataagat acgattatgc aatatatgat aataatgatg actttattga
    13261 agaattatgc aaataccatg tgagcggaca attaagaacc gctccggaac acgtttcaga
    13321 atcagtttta aaactaatgg gcaaaccttc aataaaaata tacaataagt ttgtagaaaa
    13381 gtttaatgat gtaaacaaaa agattaaaaa agagcaatac attgtacctt attttatatc
    13441 atcgcatccc ggttctcgct tgaaagatgc aattgagctt gctgaatata taagggatat
    13501 gggtcatatt cccgagcagg tacaggattt ttatcctaca ccggggacac tgtcaacatg
    13561 catgtattat acgggcttta acccattgac aaataaagaa gtttatgttc caaaagatat
    13621 agaagaaaag aaaatgcaaa gagcattgct tcaatattcc aaaaaagaaa attatgagct
    13681 ggttttgaaa gctttaaaaa aggaaaacag atatgattta atcggatacg gtcccaaagc
    13741 tttaataaag ccaagacaaa aaaagcacta aaggtgcctg gcaccgggta agggtgcctg
    13801 gcaccaacat ccggtatctt taatttaatt t
//
LOCUS       DAOSHP010000044        13801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3691_length_13801_cov_6.598501, whole genome shotgun sequence.
ACCESSION   DAOSHP010000044 DAOSHP010000000
VERSION     DAOSHP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3691_length_13801_cov_6.598501"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLB33_05700"
     CDS             <1..251
                     /locus_tag="PLB33_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63050.1"
                     /translation="RRNDGIETTYVNGSGGATDNWDSNIMAWPKPESGTAFSLDYTTD
                     DKYTVEWDVTKGSFLDGRTITVTEGVSGYTHPTTPTTP"
     gene            401..1141
                     /locus_tag="PLB33_05705"
     CDS             401..1141
                     /locus_tag="PLB33_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOG63051.1"
                     /translation="MAIIFLIYGLFIGSFLNVCVYRIPSGISIIKPPSSCGSCGHRLS
                     VIKDMLPVINYIINKGRCRYCGAHYSVRYPLIELLNGALYGLLYLKYGFSINTVLYCL
                     TASLLIVISLIDLNHKIIPDSLTGAGFVLGIIFIINGGMFFNRLAGAAIGLLLFAGIA
                     LVTNAMGGGDIKLMAVLGLIFGIKGIFFITLFSFVTGAIISLILLTLKIKSRKDEIPF
                     GPFISLAALIYIFYGVEIINWYIGILSF"
     gene            1243..2856
                     /gene="pilM"
                     /locus_tag="PLB33_05710"
     CDS             1243..2856
                     /gene="pilM"
                     /locus_tag="PLB33_05710"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOG63052.1"
                     /translation="MGKVLSLEFSNKYIKMLDGSRSGNSLTVNKSILLEVEKGCIDDG
                     KIIDIDSVINTVNEAISKNSIKSRNAIFTIHTNSTVTRNIELPVLKSKSDTMSMIKSE
                     LSQLMPVDLNQYKLIYKKTESVNADGVEKGKYIVYGLPTYMFEQYMLLAEKLKLELIA
                     FELAFNTLDKIYDKKLFINNNPLKSGVVSAFLDIGYDSLTFSVVNNGKNDFSRTSQNG
                     IKDIVKNFGAVHYLSDEEALKEIEKISLMPDDISEISKLNIVEENINIWIDELSRYIR
                     YYNSVNKEKQIEKIYLYGIYSNIKGLPEYIESHINIETVLITSLSNTVFRDKDNESIT
                     TYFTTALSLHMDKKDVNFLTDVKKQRKSTFSRRVALLGVSTAAVLAFALAAYTYYIEK
                     TALEKDIAVYDAFINNEDNIKLNSEAIALKKKTELMEKYMGEVTKLQTAIKNEDAVNI
                     IIFEQLSAAIPLGTKINSMSADIDTIKLQCSSATRQETAQFEKNLKEIEFIDNVYIPA
                     IVDGTEGSSSNYSYTVVCDLKDVISYEAE"
     gene            2843..4030
                     /locus_tag="PLB33_05715"
     CDS             2843..4030
                     /locus_tag="PLB33_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63053.1"
                     /translation="MKLSKREKFLILLLIVTVIGYLVFTYVPFGKVFNLDELKAEHSQ
                     KKQAYDTMSQNIVQKNTFEEKVLSLTEEINSLNVISDLQQEKIIVFLHNYLNKNNINA
                     SNISFTDVETVEMSHLVIPDEAKESGTLQELMYEIDGRFDALNVEYPDADKKDGNQLL
                     ARKVSTNITFDGTYADMIDFIDSIQINPVDISITSVNTIAEGDILQGTMNLNFYEIPK
                     PIDFKETNNEWIWMDLAESGKENPFSTDATQMVFASGGNYDFYMSLQPEVSDLPSILI
                     GETNDVDRKTYISKDINSVESVNFAFKTENDKYYYRYSIASSVSYPKNDEWKEFIPNS
                     EGNINIVIYSKPRILKEDSSGANISVYNSTDLKVRFDVIDDDKTSPRAYFKDARTISV
                     IRK"
     gene            4047..4736
                     /locus_tag="PLB33_05720"
     CDS             4047..4736
                     /locus_tag="PLB33_05720"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG63054.1"
                     /translation="MMRIRNNKGLTLVEILITLAVLGIVIMPLMSMFFTSQKMNNESE
                     MKYRAIQFAQEELESIKAMKILNTEDDGYPPRGDGSYGYVTSPDSEGYTIDVRIEKGA
                     EYEGSDIEPAGIPVFDRPIYIITDEDELEIDIAPGIEKIGIEIAIPYDNAKAKIRLVN
                     SDENLKVYIFKNKEHINSYKIMGDATVIEVAENSAKPDNQLYDITVTVEKDGNLIDII
                     RGTTVFEINPD"
     gene            4750..6792
                     /locus_tag="PLB33_05725"
     CDS             4750..6792
                     /locus_tag="PLB33_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63055.1"
                     /translation="MKKKFNNKGSTMIMLVMAISIISVLGAAILGITLMNYKIKKTNT
                     DLKYSFYMSESGLDRAYAAAYAIILNSIEEANESAQDKIAEFNHDKLMDILLNGSEDD
                     KDKYLEDDDGDGVYDFKQSVIDEEAKIAFENKYKEKIEDDIETAILGEGYENDDEKLI
                     ISLSAPNYGEFSADNPLRVPVSSEYENKINDIARKTDVDIVIEVPDYNKSYTVSTVTM
                     DVNPYWTKAITAKNLNIYNDDTSFNGEVFVNNNVNIGTGAIAEFKKDLSVKRYINIDK
                     NGVLDTRNVYANGIFLNGEGAEFTAEKEDDESVGYSGIMVKDDLELNAKNQVVRINGP
                     YYGFATSQAREDTDNSAIIINDKDVQLEITGDLFLYGTSYIKDIPAKYATGESLSVKG
                     NYIAYTEGLDSDDVSILDEEDRNKFLESNIIFDYDSYTPLPRLAERFKDDNSEIQVYD
                     KAKYIYAYSKNNSSSLFLGTGSIRLLGIEKALHGSAINNGSIIRSNRGIDYMAQKSGE
                     IEAVYDKKIDKLGFDTDGVSEFTTKEIFDNIKSLSSAQLAEFNNKFNPATGQLVHINN
                     NPGGEYHFPSEAKSGLIITNENIIISGEVDFTGVIICDGDITIENDPTNPGGKKFKYN
                     KGVITTLIAEHNLYELIFKDTPSTGQKTSITTFKSGDDGVNVNFSELLRFENWKIK"
     gene            6807..9362
                     /locus_tag="PLB33_05730"
     CDS             6807..9362
                     /locus_tag="PLB33_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOG63056.1"
                     /translation="MRPLKRILCIIIAILMMVTMIPGNYLFAAPGDTEDDPIPYNRND
                     TTNFPQYPNEGYVRVDKKAVWLEDEDNIAKVTMTLDGAGVKQGTDAVLVIDRSGSMDD
                     NVNVTRTYEKEVEVEKQVPYNEVQLTYNASNVSHQYEERYLLILYRWQTATSNVSITL
                     TAYVDDDGNYKGYKNVSVSGFHSTDRNYRLNSSSSSFSSWSQISNNNCANALISIFGG
                     RTAAATVKGKNVNVIFPTTRSLLQSTQSSYTVMVKEMQTISETTSVKKMNNAKEAANA
                     FVDALLPEGNEDTLNRIAVVSYSSSGYGKGTAFEDSALTNDNASLKSAINGIAATGGT
                     HIQAGIKMAQDILSESNAVNKYIVLVSDGEPTFSYRANAARQRTQTDLDLNYPSDTNY
                     VLTSFTTNVVGSGSDYGYYKSGWLGGFDYRYYVGSYRVIDNGIPTISQALMAKQAGIK
                     IYTVGFDVSNNSDAKYTMEYVASDKDMFYLTTDDLSGVFTEIAGRIAKAGTDARVNGL
                     ASISSDGGYYFTILNDSKHPITAKPGSATLNDNAITWNLGDITESAAELTYYIKLNLS
                     GDIVLPPDAMLSTGEIASVVYKNYRGVWVERTFPDPKLSAGGGTLNVKYYLSDEDGKP
                     INNDGEVIPYKERVILYDNEGESIGLGETISASSYAKPFKDHEVQSNTAQDENGNTLS
                     DSILVTRATIYLNFPYYPMPKESKLKNFSMYKFDGNEPAPTAGLETIEKMTYIFGLKL
                     ELGSKELELNISADNGGNIDVNSFRLYDENNDIMNFYDYMFVSKVGNKCILGFKSDTD
                     MESEKDYTIIYNVKNNNVGNYKMYIDGGDLLVSEPESVSKELQIEVTKTKDIQ"
     gene            9425..9907
                     /locus_tag="PLB33_05735"
     CDS             9425..9907
                     /locus_tag="PLB33_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG63057.1"
                     /translation="MKNKNGFTLIELIVILALASIVMLGVMSFLITNYRSYNVINTES
                     ELQYQSQYIINYITERILEANKFEANTFYYNDDSEVTFTFDADETRDEYKNRIEYRHI
                     DGEGNEITKIPLGNYLIGLDIDISEVTESEVVIELTLQKDRSEPYTAKQTVFLRNYKK
                     "
     gene            10111..11547
                     /locus_tag="PLB33_05740"
     CDS             10111..11547
                     /locus_tag="PLB33_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63058.1"
                     /translation="MFLSLEFGNNIKIVEGYKKKNIVYIKKAVTINESVDIKDILKLS
                     DSINEVLIKNNIRTKRAIFIINTESAIIRKIKLPLLNKKSEIITMIKHELGQVVSADL
                     SLYKIIYKIIETHEAEEKATALYAAYCLPLNIYEFYEKLGKKLRLKVVGIDISSNCLN
                     NILEQNIELNNHILDSKDAYAFVNTHFDKILFSVINKGNDFFMISNIERNHGNMVETD
                     HRHDRDDMVIRVEYINKCCRYYHSVNNKKVNRIYLYGYNSSDGLDKLLSSILNLEVEY
                     INSVSNISIADNISINEYFISLMALYNAKSSFLTKNEKRIQILINFAGASVILFCLLF
                     FLSKHSNSVRKQIAYLDIYIKNEKNIERNLIIEKLKSEIRLLNSAITSIETAMDLVNN
                     ENVRTENIKGVYNSLPGNTEVLSFSIDKDSLNMQCISDSMDEVLCLLRNLEGLYFVES
                     VYIPAIQSMKDMKYSYTIICNFKVNYEG"
     gene            11537..12088
                     /locus_tag="PLB33_05745"
     CDS             11537..12088
                     /locus_tag="PLB33_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63059.1"
                     /translation="MKDKYLVFIIVLFVIAILYGLWPLYCKFNPAQNKLNTSVEAYKS
                     MMDNFRSEDSIKSKKIDYINKINKSDFRSQLSQEKIISVLYTCALKNNIRISDIKFVE
                     TDDAPDGDIDIIESKEDFVFETRCVIAEFKAGLGQLLQFIDEIKSFRDISVINLDVSI
                     WEEDIVFAFVHMKFYAVPVDMNL"
     gene            12144..12530
                     /locus_tag="PLB33_05750"
     CDS             12144..12530
                     /locus_tag="PLB33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG63060.1"
                     /translation="MNKLFNNRGLTLAEVIMTMAILGIVICPLMNLLVLSQNINSEGE
                     KEYDVIQTAQYYMEETRAMGEIDPEIFVYNSEEMSYERMVSDVLDDLSAEIKITPFSY
                     GLHYIEVNILNDGEVINSLTGSIVID"
     gene            12621..13160
                     /locus_tag="PLB33_05755"
     CDS             12621..13160
                     /locus_tag="PLB33_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63061.1"
                     /translation="MNNKGSTLVLLIIVIALVIVLGASVLNIAVKQYAIKKFNIDSKQ
                     AFYFSETGLNEAYVKSCALIEESIIKALQITEDYISINSFNEQAENIFVTSYKIYIGT
                     NIENRIETASNPKVKVWNDTLVFIDNALTLELKSSYIHNDIDKVTGVELVIGVPDYHD
                     VSEGSYDVRDYIKFKNWNS"
     gene            13284..>13801
                     /locus_tag="PLB33_05760"
     CDS             13284..>13801
                     /locus_tag="PLB33_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOG63062.1"
                     /translation="MKNKGFSLVELLVSLGISSIIIGLIFSFFIINFKGYKSVRNDFE
                     MQFQAQYILNFMAGRIVDSNSVSFARVNTQNYSMTAVRSAGAEYPIHKISFKYGSESE
                     NYVFHIINNTIRYGKGEKEMNPTVELGNYVDEMYVSLFKDESFQDAKAVKLKLVMKKD
                     EQLHEAFQAVYM"
BASE COUNT         4989 a         2014 c         2705 g         4093 t
ORIGIN      
        1 caagaagaaa tgacggcatt gaaactactt atgttaatgg ttctggtggt gctaccgata
       61 attgggattc taacataatg gcatggccga aacccgaatc cggtacagct ttttcactag
      121 attacacaac agatgacaaa tataccgttg aatgggatgt tactaaaggt tcatttcttg
      181 atggaagaac aataactgta actgaaggtg tatctggtta tactcatcca actacaccaa
      241 ctacaccttg atgagacatg tgagtgacac ggggacgcat gacacgggga cggggtcttg
      301 tcacaaactg tgcgagaggt atatccccct tgtcttgcaa gcataagaaa cgaaaaattg
      361 gggagaggca ttaatccttt caaccaaaca ggaaatcaca atggcaataa tatttttaat
      421 ctacggcctc tttataggct cattcctgaa tgtatgcgta taccgcatac cttcaggtat
      481 ttcaataata aaaccgccct cctcctgcgg gagctgcgga cacaggctgt cagtcataaa
      541 ggacatgctt cccgtaatta attacataat aaacaaggga aggtgcaggt actgcggcgc
      601 acattattcc gtaaggtacc cgcttataga attgttaaac ggtgcacttt atggcttgct
      661 gtatctaaaa tacggcttta gcataaacac cgtgctttac tgcctgacgg caagcttact
      721 catagtgata agtttaattg atttaaatca caaaataata cccgacagct taacaggagc
      781 agggtttgtt ttaggaatta tatttataat aaacggcgga atgtttttta acaggctggc
      841 gggggcagca atcggcttgc tgctgtttgc gggaattgcc cttgtaacaa atgccatggg
      901 cggcggagac ataaagctta tggcagttct gggattaatt ttcggtataa aagggatttt
      961 tttcataacc ttgttttcct ttgttaccgg agcaataatt tcattaatac tacttacttt
     1021 gaaaattaaa agcaggaaag acgaaatacc ttttggacct tttatatctt tagctgcttt
     1081 aatatatata ttttatggag ttgaaataat aaattggtat atcggaattt tgtcattctg
     1141 agggctttag cccgaagaat ctcattttaa acttgagatc cttcgcttac gctcaggatg
     1201 acaaagcaag gaacaaaaaa atctataaga aaggtaaaag acatggggaa agttttatct
     1261 ttagaattca gcaacaaata tattaaaatg ttggacggct caaggagcgg gaattcacta
     1321 actgttaata aaagtatttt gttggaagtg gaaaaaggat gtattgatga cggtaaaatt
     1381 attgatattg attcagtaat taatacggtc aatgaagcca taagcaaaaa cagcataaaa
     1441 tcaagaaatg caatttttac cattcataca aattcaaccg ttacaagaaa catagaactg
     1501 cctgttttaa aaagcaagtc agacactatg tccatgatta aaagcgaatt aagccagctt
     1561 atgcctgttg atttgaatca atacaagctg atttacaaaa agactgagtc tgttaatgca
     1621 gacggtgtgg aaaaaggcaa atatattgtt tacggcctcc ctacatacat gtttgagcag
     1681 tatatgctct tggccgaaaa gcttaagctt gaacttattg catttgaact tgcatttaac
     1741 actttggata aaatttatga caaaaaactt ttcataaata acaatccttt aaaatccggt
     1801 gtcgtttctg cattcttgga tataggttat gacagtctga cattcagtgt tgtaaataac
     1861 ggaaaaaacg atttttccag aacctcccaa aacggcataa aggacattgt taaaaatttt
     1921 ggtgcggttc attatttaag tgacgaggag gcattaaaag aaatagaaaa aatatcatta
     1981 atgcctgatg atatttccga aatttcaaaa cttaatattg tcgaggaaaa cattaatatt
     2041 tggattgatg agctttcaag gtatattagg tattacaatt ccgtaaataa ggaaaaacag
     2101 atagaaaaga tttatttgta cggcatatat tcaaatataa agggattgcc ggaatatatc
     2161 gaatcgcata taaatattga aacagtgctt attacatcat tgtcaaacac tgttttcaga
     2221 gataaggata atgaaagtat tacaacatat ttcactactg ccctatcctt acatatggat
     2281 aagaaggatg taaacttttt aacagatgtg aaaaaacaaa gaaaaagcac attcagcaga
     2341 agagttgccc ttctcggagt ttcaacggcg gcagttttag cctttgctct tgccgcatat
     2401 acttattata tagagaagac agccttagaa aaagacatag cggtttatga tgcttttata
     2461 aacaatgaag acaatatcaa gcttaacagc gaagcaattg ctttaaaaaa gaaaactgag
     2521 ctgatggaga aatatatggg tgaagtaaca aaacttcaaa cggcgataaa gaatgaggat
     2581 gccgtaaata taataatatt tgaacagctt tcggcagcca ttcctcttgg tactaaaata
     2641 aattcaatga gtgccgatat tgataccata aagctgcagt gctcttctgc caccagacag
     2701 gaaaccgctc aatttgagaa aaaccttaag gaaattgaat ttatagataa tgtctacata
     2761 ccggctatag ttgacggaac agaaggaagc agttccaatt actcctatac cgttgtttgt
     2821 gacttaaagg atgtgatatc ttatgaagct gagtaagaga gagaaatttt taatattgct
     2881 gttaattgtt acagtaattg ggtatttagt atttacatac gtaccgtttg gaaaagtttt
     2941 taacctggat gaacttaagg cagagcattc acaaaaaaag caggcatatg atactatgtc
     3001 gcagaatatt gtgcaaaaaa atacttttga agaaaaagtt ctgtctttaa ctgaggagat
     3061 taacagctta aatgttattt cagacttgca gcaggaaaaa ataattgtat ttttacataa
     3121 ttatttgaat aaaaacaata ttaatgcaag caacataagc tttacggatg tagaaacggt
     3181 tgagatgagt catcttgtaa ttcccgatga ggcaaaagaa tcaggcactc ttcaggaatt
     3241 aatgtatgaa attgacggaa gattcgatgc tctaaacgtt gagtatcctg atgcagataa
     3301 aaaggatgga aatcaactgt tggcacggaa agtatccact aatataacat tcgatgggac
     3361 atatgccgat atgattgatt ttatcgactc aatacaaatc aatcctgtag atatatcaat
     3421 tacaagtgta aataccattg cggaaggcga tatacttcag ggaacaatga atttgaactt
     3481 ctacgaaatt cctaaaccga tagacttcaa agaaactaac aatgagtgga tatggatgga
     3541 tttggctgag tcgggtaaag aaaatccttt ttcaacggat gcaactcaaa tggtttttgc
     3601 atcagggggc aattatgatt tttatatgag cctgcagcct gaagtttccg atttaccctc
     3661 gattttgata ggtgaaacaa atgatgtgga caggaaaact tatatatcta aggatataaa
     3721 ttcagtggaa agtgtaaact ttgctttcaa aaccgaaaac gacaagtatt attacagata
     3781 ctccatagca agcagtgtat catatccgaa aaatgatgag tggaaagaat ttattcctaa
     3841 ttcggaagga aatataaata ttgtaatata ttcaaaaccg aggattttga aggaagactc
     3901 ctcaggagcc aatatatccg tatataattc aacggatttg aaggttagat ttgatgttat
     3961 cgatgatgac aaaacaagcc cgagggcata tttcaaagat gcaagaacaa taagtgtaat
     4021 aagaaagtag atggagaggc agtacgatga tgaggataag aaacaataaa ggattaacat
     4081 tggtggaaat attgataacc cttgcagtgt tgggtatagt tattatgccc cttatgtcta
     4141 tgttttttac ctctcaaaaa atgaacaatg aaagcgaaat gaagtacaga gcaattcagt
     4201 ttgcccagga agagttagaa tctattaagg caatgaagat tctgaatacc gaagacgacg
     4261 gatatcctcc cagaggggac ggaagctacg gttatgtgac ctcacctgat tcggaaggtt
     4321 acaccataga tgttaggatt gaaaaaggag ctgaatatga aggcagcgat attgagcctg
     4381 ccggtatacc cgtgtttgac aggccaatat acataatcac cgatgaagat gaacttgaaa
     4441 ttgatattgc tccgggaatc gagaaaatcg gtatcgaaat tgccattcct tacgataatg
     4501 caaaagcgaa aataaggttg gtaaattcgg acgaaaattt aaaagtatat attttcaaaa
     4561 ataaggagca tataaatagt tataagataa tgggtgatgc aactgttatt gaagttgctg
     4621 aaaattccgc aaaaccggac aatcaactgt atgatataac ggtgactgtt gaaaaagacg
     4681 gcaatttaat cgatataata aggggaacaa ccgtatttga aatcaatcct gattagagaa
     4741 ggtgatataa tgaaaaagaa atttaacaat aaaggctcca ccatgattat gctggttatg
     4801 gcaatatcta taatttccgt tttaggtgca gcaatattag gcataactct aatgaattat
     4861 aagataaaga aaaccaatac ggatttgaaa tattcatttt atatgtctga atcgggactt
     4921 gacagagcat atgccgcagc ctatgcaata attctaaatt ccattgaaga agcaaatgaa
     4981 agtgctcagg ataaaattgc agaatttaat catgacaagc ttatggacat attattaaac
     5041 ggctctgaag atgataaaga caaatatctt gaggacgatg acggagacgg cgtttatgat
     5101 tttaagcaat cagttattga tgaggaagca aaaatagcct ttgaaaataa gtataaggaa
     5161 aagattgaag atgatataga gactgcaata ctaggtgaag gatacgaaaa tgatgatgaa
     5221 aaactgataa tatctttatc agcgccaaat tatggcgaat tcagcgcaga caatcctttg
     5281 cgagtacctg taagctctga atatgaaaat aaaataaatg atatagccag gaaaacagat
     5341 gttgatattg taattgaggt gcctgattat aacaaatcct atactgtgtc taccgtaaca
     5401 atggatgtta acccatactg gacaaaggca atcaccgcaa agaacctaaa catttacaat
     5461 gatgacacca gttttaacgg ggaggtattc gttaataata acgttaatat aggtactggt
     5521 gcaatagctg aatttaaaaa ggatttgtcc gtaaaaaggt atattaacat agataaaaac
     5581 ggtgtcttag atacaagaaa tgtatatgca aacggaatct ttttaaatgg cgaaggtgct
     5641 gagtttacgg cagagaaaga agacgacgaa agtgtcggtt attccgggat tatggtaaaa
     5701 gacgatttgg aattaaatgc aaaaaaccag gttgtcagaa ttaacggtcc atactatggc
     5761 ttcgctacca gccaggcaag agaggatacc gacaacagtg caataattat taatgataag
     5821 gatgttcaat tagaaataac aggcgactta ttcttatacg gcacttcata tataaaggat
     5881 atacctgcaa aatatgccac gggggaatcc ctgtcggtaa aaggaaatta tattgcctat
     5941 accgagggtt tggattctga tgatgtaagt attttggatg aagaggatag aaacaaattt
     6001 cttgaatcca atattatatt cgattatgac agttatactc ccttgcccag actcgcagaa
     6061 agatttaagg atgataatag tgaaatacag gtatatgata aggcaaaata catatatgca
     6121 tacagtaaaa acaactcttc atctttattt ttgggaacgg gctcaataag acttttaggt
     6181 attgaaaaag cattgcacgg ctcagccata aataacggaa gcataattcg ttcaaataga
     6241 ggaattgatt atatggcaca aaaaagcggt gaaattgaag cggtatacga taaaaaaatc
     6301 gataaactcg gatttgatac tgatggtgtg agtgagttta ctacaaagga aatatttgat
     6361 aatattaaaa gtttatcttc tgctcaatta gccgaattta ataacaaatt taatccggct
     6421 acaggtcagt tggtgcatat aaataataat ccgggaggag aataccattt tccttcggaa
     6481 gcaaaaagcg gattgattat aacaaatgaa aatattataa tatcaggaga agtggatttt
     6541 accggtgtaa taatatgtga cggtgatatt acaattgaaa atgatcccac aaatcccggc
     6601 ggcaaaaaat ttaaatacaa taagggagtt attacgactc tcattgcgga gcataatctt
     6661 tacgagttaa tatttaagga tactcctagc acgggacaaa aaacaagcat tactacattt
     6721 aaatcaggag atgacggcgt aaatgttaat ttcagcgaat tattgagatt tgaaaactgg
     6781 aaaataaaat aaagaggggg taaaaaatga gacctttaaa aagaatttta tgcattataa
     6841 ttgcaatttt gatgatggtt acgatgatac cgggaaacta tttatttgca gcaccgggag
     6901 atactgagga tgaccccatt ccttataacc gcaatgatac taccaatttt ccgcaatatc
     6961 caaatgaggg gtacgtacgt gtcgataaaa aagcagtatg gttagaggat gaggataaca
     7021 ttgccaaagt taccatgaca ttggacggtg caggtgtaaa gcaaggaaca gatgcggtac
     7081 ttgttataga ccgttccggc agtatggatg ataatgttaa tgtaacacgt acttatgaaa
     7141 aagaggttga agtagaaaaa caagttccct ataatgaagt acagttgact tacaatgctt
     7201 caaatgtcag tcatcagtat gaagagagat acctattgat tttgtataga tggcagactg
     7261 ctacttctaa tgtttctata acgctgactg catatgtgga tgatgacggc aactataagg
     7321 gatataaaaa tgtttccgta agcgggtttc attctacaga cagaaattac agattaaatt
     7381 caagcagcag ttcatttagc agctggtcac agataagcaa caataactgt gctaatgcat
     7441 taataagtat attcggagga aggactgcag cggcaacagt taaaggcaaa aatgtaaacg
     7501 tgatatttcc aactacaagg agtttattgc agtccacaca atcaagctat acagtaatgg
     7561 ttaaagaaat gcagactatt tcggaaacaa caagtgttaa aaagatgaat aatgcaaagg
     7621 aagcagccaa tgcatttgta gatgctttgc ttcctgaagg aaatgaagat acattgaaca
     7681 gaatagccgt tgtatcttac tcaagcagtg gctacggcaa aggtacggca ttcgaagatt
     7741 cagctcttac caatgataat gcttccttaa aaagtgctat taatggaatt gcagcaacag
     7801 gcggaaccca tatacaggca ggtatcaaaa tggcgcagga tatattatct gaaagcaacg
     7861 ctgttaataa atatatagta ttggttagtg acggcgagcc tacctttagt tatagggcaa
     7921 atgcagcaag acaacgtacg cagaccgatt tagatttgaa ttatccttct gacactaatt
     7981 atgttcttac ctcatttact acaaatgttg taggctccgg aagtgattat ggatattata
     8041 aatccggatg gttaggagga tttgattaca gatattacgt gggaagctac agagtaattg
     8101 acaatggcat accaacaatt tcacaggctt tgatggctaa acaagcaggc attaaaatat
     8161 acactgtagg ctttgatgtg tcaaataatt ccgatgcaaa atatacaatg gaatatgtcg
     8221 cttcagataa agatatgttt tatcttacta cagatgattt gagcggagtg tttaccgaaa
     8281 ttgcagggcg tattgccaaa gcagggactg atgcccgtgt aaatgggctg gcaagcatca
     8341 gcagtgacgg aggatattat tttactatat tgaacgactc aaagcatcct ataaccgcca
     8401 agccgggctc tgcaacctta aacgacaatg caataacatg gaatttaggt gatataaccg
     8461 aatctgcggc agagctgaca tattatatta agcttaattt atcaggcgac attgtattgc
     8521 cacctgatgc aatgcttagc acaggcgaga ttgcatcggt tgtgtataaa aattacagag
     8581 gtgtttgggt tgaaagaaca ttccctgacc cgaaactgtc tgcaggcggt ggcaccttaa
     8641 atgttaaata ctacctttct gatgaggatg gtaaacctat aaataatgat ggagaggtaa
     8701 tcccctacaa ggaacgtgta atcctatatg acaacgaagg agaatcaatt ggtttaggag
     8761 aaacgatatc tgcatcttca tatgcaaaac catttaagga ccacgaagtt cagtctaata
     8821 ctgcccaaga cgaaaatgga aatactctat ctgacagcat acttgtgaca agagcaacaa
     8881 tttatttgaa tttcccttat tatccgatgc cgaaggaaag taagctgaaa aacttttcaa
     8941 tgtataaatt tgacggcaat gaacctgcac cgacagccgg cttggaaacg attgaaaaaa
     9001 tgacatatat cttcgggtta aagcttgaac ttggaagcaa ggagttagaa ttaaatattt
     9061 ctgctgacaa tggaggaaat attgatgtca acagttttag gctttatgat gaaaataatg
     9121 atataatgaa tttttacgat tatatgttcg tttcaaaggt tggaaataag tgtattttag
     9181 gttttaaaag tgataccgat atggagtccg aaaaagatta cacaataatt tacaatgtga
     9241 aaaataataa tgtcggtaat tacaaaatgt atattgacgg tggagactta ttggtatctg
     9301 aacctgagtc agtgagcaaa gaattgcaaa ttgaagtaac aaaaacaaaa gatattcagt
     9361 aatcgctcaa taaatgaaat tttgggcgaa tgctcaaaat ttcattttaa tatgaggaat
     9421 gtatatgaaa aataaaaatg gttttacatt aattgaactt attgttattt tggcattagc
     9481 ttcgatagtg atgttaggtg ttatgtcttt tcttattaca aattacagaa gttataatgt
     9541 gataaataca gagtcggagc ttcaatacca atctcagtat attattaatt atataaccga
     9601 aagaatattg gaagctaaca aatttgaagc caataccttt tattacaatg atgatagtga
     9661 ggttactttt acttttgatg ctgatgaaac aagggatgaa tataaaaata gaattgaata
     9721 ccgtcatata gatggagaag ggaatgaaat aactaaaatt cccttaggta attatctaat
     9781 aggactcgat atagatattt cggaagtaac agaatcagaa gttgttatag aattaactct
     9841 tcaaaaagac agaagcgagc catacacagc taagcagacg gtattcctga gaaactataa
     9901 aaaataatca gcttaaaaaa acaaacccca gataagtatt cgggtttgtt tttctatgtc
     9961 tttcaatact attgccaatt attattaaaa tttatttaca cattataaaa atataaaaca
    10021 aattgctaaa atataattaa caatataaat tttgacatta tcttccagag agacattata
    10081 ttataactaa aaatattata aggtgagttt atgtttttgt ccctggaatt cggcaataat
    10141 atcaaaattg ttgaaggata taagaaaaaa aatattgttt acataaaaaa ggctgttaca
    10201 atcaatgaat ctgttgatat aaaggatatc cttaaattat ccgattcaat aaatgaagtg
    10261 ttgataaaaa acaatataag aactaaaaga gcaattttca ttattaacac cgaatcagct
    10321 attatcagaa aaatcaaatt gcctttatta aataaaaaat cagaaataat aaccatgata
    10381 aagcatgagc tgggtcaggt tgtttccgcc gatttaagcc tgtacaaaat tatatataaa
    10441 attattgaaa ctcatgaagc ggaagaaaag gcaacggctt tgtatgcagc atattgtctt
    10501 cctttaaata tatatgaatt ctatgaaaaa ctgggcaaga aattaaggct taaagtcgtt
    10561 ggcatagaca tatcttctaa ttgcctcaac aatatacttg aacaaaatat agaattgaat
    10621 aatcatattt tggactctaa agatgcgtat gcttttgtta atactcattt tgataaaata
    10681 ttgttctctg tcataaataa aggtaatgat tttttcatga tttcaaatat agaaagaaat
    10741 catggcaata tggttgaaac tgaccatagg catgaccggg atgatatggt tatccgggtt
    10801 gaatatataa acaaatgctg caggtattat cattccgtaa ataacaaaaa agtaaacaga
    10861 atatatttgt acgggtataa tagctctgat ggtttggaca agcttttgtc atccattctg
    10921 aatctggaag tcgaatacat aaactctgtt tctaatatct caattgctga taatatcagt
    10981 ataaatgagt atttcatatc tttaatggca ttgtataatg ctaaatcatc atttctgaca
    11041 aaaaatgaaa aaagaataca aattttaata aactttgccg gagcatcggt gatacttttt
    11101 tgtttgcttt tctttttatc aaagcattca aactcagtca ggaaacagat tgcatattta
    11161 gacatttaca ttaaaaatga aaaaaatatt gagagaaatc ttataattga aaaactaaaa
    11221 tcagaaatcc gattattaaa ttcagccata acatctattg aaacagctat ggatttggtt
    11281 aacaatgaaa atgtcagaac tgaaaatata aaaggagtat ataattcact ccccggcaat
    11341 actgaggttt tatcattttc aattgataag gatagtttaa atatgcagtg tatatctgat
    11401 tccatggatg aagtactctg tttgttgaga aatttggaag gattatattt tgtggaaagt
    11461 gtttacattc ctgcaattca atcaatgaag gacatgaagt attcatacac gataatttgt
    11521 aatttcaagg ttaattatga aggataaata tttagttttt attattgtat tatttgttat
    11581 agctatactt tacggcttat ggcccttgta ctgtaaattt aatccggctc aaaacaagct
    11641 gaatacttct gttgaagctt ataaatccat gatggacaat tttcggtcag aggattctat
    11701 caagtctaaa aagattgatt acataaataa aataaacaaa tcagatttca ggtctcagtt
    11761 aagtcaggaa aaaattattt ctgttttata tacctgtgca ttgaaaaaca atattcgtat
    11821 ttcagatatt aaatttgtag aaacagatga tgctccggat ggagatattg atattataga
    11881 atcgaaagag gattttgttt ttgaaaccag gtgcgtaata gcagagttta aagccggatt
    11941 gggacaactt ttgcagttca tagatgaaat taaaagtttt agagatatca gcgtaattaa
    12001 tttagatgta tcaatatggg aggaagatat tgtttttgca tttgttcata tgaagtttta
    12061 tgcagttcct gtagatatga atttatgaag cagaacattt tgttttttgc agcgtccggg
    12121 aaaaatttgt atggggtggt attttgaata aattatttaa taacagagga ttgaccttgg
    12181 cagaggtaat tatgacaatg gcaatacttg gaattgtaat ctgccctctg atgaatttat
    12241 tggttttgtc gcagaatata aacagtgaag gtgaaaaaga atacgatgta attcaaacag
    12301 ctcaatacta tatggaagaa acaagagcaa tgggtgaaat tgacccggaa attttcgttt
    12361 ataacagtga agaaatgtct tatgaaagga tggtttcgga cgttttggat gatttaagcg
    12421 cagaaatcaa aattaccccc tttagttacg gactgcacta tatagaagtt aatattttaa
    12481 atgatggaga agtaatcaac agcttaacag ggagtatagt tatagattga tgagatcctt
    12541 cgcttatgct caggatgaca accctttgtg tcattctgag agctttagct cgaagaatct
    12601 catgtttaac aggagtatgt atgaataata aaggttctac cttagtactt ttgataattg
    12661 taattgcact ggtaattgtt ttgggagctt ccgttttgaa tatcgcagtt aaacaatatg
    12721 caataaaaaa gttcaacatt gattccaaac aagcatttta tttttctgaa accgggctca
    12781 atgaagccta tgtcaaatca tgcgctttaa ttgaagaatc aattattaaa gcattgcaaa
    12841 taactgaaga ttatatatca ataaattcat ttaatgaaca agcagaaaat atttttgtta
    12901 caagttataa aatttatata ggaacaaata ttgaaaatag aattgaaacc gcatccaatc
    12961 ctaaagtaaa agtatggaat gatactttgg tttttattga taatgcactt acgttagaat
    13021 tgaaatcttc ttatattcat aacgacattg ataaggttac gggagttgag ctggttatcg
    13081 gtgtaccgga ctaccatgat gtatcagagg gttcctatga tgtaagagat tatataaaat
    13141 ttaagaattg gaacagttaa aagctgagat ccttcgctat cgctcaggat gacaatttcg
    13201 tcatttttgt taataattgg ctttaagggt caagtcatcg tcattctgaa cacagtaaag
    13261 aatatcatat aaaaggggtt cttatgaaaa ataagggttt ttcattggtt gaacttttgg
    13321 tgtcattagg gatatcctct attattatag ggttaatttt ctcttttttt ataattaatt
    13381 ttaaaggtta taaatcagtg agaaatgatt tcgaaatgca attccaggca caatatatat
    13441 taaactttat ggccggaagg attgttgatt ctaattctgt gagttttgca agagtaaaca
    13501 cgcaaaatta ttccatgact gctgtaagaa gcgccggagc agaatacccc atacacaaaa
    13561 tttcttttaa atatggaagt gaaagtgaaa attacgtatt tcatattata aacaacacca
    13621 ttcgctatgg gaagggagaa aaggaaatga atccaacagt tgagcttggc aattatgtgg
    13681 atgaaatgta tgtttcatta tttaaggatg aaagttttca agatgcaaag gcagtaaaat
    13741 taaaactggt tatgaaaaag gatgagcagt tgcatgaagc ttttcaagcg gtttatatga
    13801 g
//
LOCUS       DAOSHP010000045        13789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3694_length_13789_cov_9.585845, whole genome shotgun sequence.
ACCESSION   DAOSHP010000045 DAOSHP010000000
VERSION     DAOSHP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3694_length_13789_cov_9.585845"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLB33_05765"
     CDS             <1..516
                     /locus_tag="PLB33_05765"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOG63063.1"
                     /translation="KMTIDAYKSMAKKVNYPLHLGITHAGTLFEGSIRSAIGVGSLLA
                     DGIGDTIRISLTDDPREEVKVAREILKSLNLRNFGINYITCPTCGRTKIQLIELSKKI
                     QEGLKNVNKPITVAIMGCAVNGPGEARQADIGIAGGKGEALLFKKGEIIEKVKEEDLV
                     KRLLEEIDKLS"
     gene            638..1246
                     /locus_tag="PLB33_05770"
     CDS             638..1246
                     /locus_tag="PLB33_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOG63064.1"
                     /translation="MAVGTLNVEKRLETNSRASNRLRRQGYLPGNISRKGKDSVSVTV
                     KADELRKGLSTYGRNALFKITLDDTEFTGMVKDIQLSPVKGSMMHVDFQEVSLNEEIK
                     VQLSIVLKGTEALEFKGLIALTQLDSITVKGLPQNIPDDLTIDVSNIEKVENICLKDV
                     KFTEGIVPEGHPEQVLISIVEAKRTVEEEETGEETDDEVAGE"
     gene            complement(1298..1918)
                     /locus_tag="PLB33_05775"
     CDS             complement(1298..1918)
                     /locus_tag="PLB33_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOG63065.1"
                     /translation="MICGGIIGLERSRKNRPAGFRTYMIVCLSSALVMMTNQYIYNNF
                     SGSDPARLGAQVISGIGFLGAGTIMITSRNQIKGLTSAAGLWASACLGLAIGVGFYSG
                     AVIVALIVFIIITFFKKIDYMLTSNNRIINIYTSFKSIEQLDKFIIFCNLSDFKVRNI
                     EITKDTIPHEISVIAIITLEAKERMNHVDVIQKLNSFEGLVHIEEL"
     gene            2120..6358
                     /locus_tag="PLB33_05780"
     CDS             2120..6358
                     /locus_tag="PLB33_05780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOG63066.1"
                     /translation="MLFNKIFTDESLDKIKIQKIEVIERTNTINLHALSQEIIDFNHI
                     ENIKDEISKHYNNSVNVEIYLKYKLEKNINIEKIKENLLYIINKSSNILSKAKDNIKI
                     EFKDECFFVIADNKLLIDELNDNNTERKLKKLICDYNLDYNIKAIYNEAEEEAAITAQ
                     RIFDQALKKNLEIIKNNSSEKKPANKSQEGSYKKRSRKKIDFDSIPVSLISELTNDSG
                     VVNIKGKIVNVDKRELKNGSFLISLIITDSTDSIMCKYFCPNGFDDEGIENGNYIQIA
                     GNVEYDDYEKELIVKILDLQLTTEEIKRMDNAKEKRVELHLHTGMSSMDGINSFKEYA
                     KKARQWGHKAIAITDHGVVQGYPEAMDCADDNFKVIYGMEGYLVNDTVSIVQNCQNVN
                     LDSVFVVFDIETTGLNPKNDNIIEIGAVKISNRKVIDSFSTFVHIDKKLPGKIIELTS
                     ITDDMLQGQPEIDEALPAFLDFAKECVLVAHNAKFDLGFIKENTKVLSIENYNPPVLD
                     TLELSKALIKDVKNHRLNTLTKKLGINLINHHRAVDDANATGQLLIILLNKLSEREIY
                     DVSKLNDILMEDIDIVKQPSYHINILVTNYIGLKNLYSLVSDSHINNFYKKPRLLKSN
                     LNQLREGLLLGTACESGELFSAFLEGKSDEKIEEIADYYDFYEIQPLDNNMFLVNNER
                     LRSVEELKIINKKIVELGERKGKTVVATGDVHFLEPEDEIFRRILMSGQGYEDAEEQA
                     PLYFKTTDEMLDDFSYLGEEKAYEVVVTNTNLIADLIEPILPIPNGTFPPIMEGSDNE
                     LKNICFSKAKSIYGEKLPEIVESRLNRELNSIIGNGYSVMYMIAQKLVKKSNEDGYLV
                     GSRGSVGSSFAATMAGITEVNPLVPHYVCNKCKYSEFIEDGSYGSGFDLPDKHCPNCG
                     TLLVKDGHDIPFEVFLGFEGDKEPDIDLNFAGEEQGICMKYTEELFGEGKVFRAGTIS
                     TIADKTAYGFVKNYFEERGITKRKAEIERLIQGCLGIKRTSGQHPGGVMIVPKDKDIH
                     DFTPVQYPANNEKSGTITTHFDYHSISGRILKLDLLGHDTPSIIRMLEDLTGLESKDI
                     PMDDPSTMAIFNSPEILGISLDEINCSTGTLAIPEFGTSFVRQMIKDTKPKSFSDLVR
                     ISGLSHGTDVWINNAQDIIKKGYAKIGGVISTRDDIMTYLIQMGVEKKTAFDIMERVR
                     KGKGLRKEDEEAMKAQNVPQWYIDSCNTIKYMFPKAHAVAYVTMSVKIAYYKVHYPEA
                     FYATYFTTKVDDFDADIVMKGQNHILNTIKSFEEKGNDKTAKEKNLITVLEVAYEMYK
                     RGFKFTKVDLYKSDDRKFLLTEDGILPPLMGLQGLGANAAANIKAEREISEFISIEDI
                     INRAKVSKTVIEVLEKHGCLKNMDETNQISIFNV"
     gene            6641..7105
                     /gene="rimP"
                     /locus_tag="PLB33_05785"
     CDS             6641..7105
                     /gene="rimP"
                     /locus_tag="PLB33_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOG63067.1"
                     /translation="MNKKDIVSSVRKITEEILKSTDIELFDIEYVKEGPFKYLKVYID
                     KPGGITVDDTADISRVLNKKLDEVDLIKEQYFLEVSSPGVERPFKTEKDYLKNLNKLV
                     EARLYKPIDDKKTVKGILTEKNENNIVIKSGENNFTIELKDLAKINRVIEDF"
     gene            7118..>7563
                     /locus_tag="PLB33_05790"
     CDS             7118..>7563
                     /locus_tag="PLB33_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HOG63068.1"
                     /translation="MNKDILNALSELEREKGILKDVVLEAIRSALETGYKKNYGKATN
                     FSIEIDKTTGEYKLFQDKTVVEKVENKNLEISLEEAKSIDSKYELGDIVKIQIEPKED
                     FGRIAAQTARQVILQKIREAERESIFSEFYGRESDILTGIVQRENKG"
     assembly_gap    7564..7663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7664..8050
                     /gene="nusA"
                     /locus_tag="PLB33_05795"
     CDS             <7664..8050
                     /gene="nusA"
                     /locus_tag="PLB33_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226977.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HOG63069.1"
                     /translation="SIAREAGSRTKIAVFSHDPNVEPIGSCVGNKGTRVKNIIDELNQ
                     EKIDIIIYEKDPEKFIANSLGPAKILKVTANEKEKTAVTVVPDNQLSLAIGKEGQNVR
                     LAAKLTGWKIDIISESQYNDSLREEV"
     gene            8047..8316
                     /locus_tag="PLB33_05800"
     CDS             8047..8316
                     /locus_tag="PLB33_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOG63070.1"
                     /translation="MKVRKIPMRRCIGCMESFPKKELCRIVKTKENEIFFDETGKKNG
                     RGTYICKNIECFNKAIKSKRLAKSLETEIPEEVIEAIKEAIEKNA"
     gene            8309..8602
                     /locus_tag="PLB33_05805"
     CDS             8309..8602
                     /locus_tag="PLB33_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOG63071.1"
                     /translation="MHKIYSMLGIARKGGNVSIGFDSTKADIEKNNSFLVIIAEDASD
                     KTKKNIKFVCDKYNCEYIETGEKELLGKALGKKVVSVLSIRDRNIASYLLNNV"
     gene            8618..10816
                     /gene="infB"
                     /locus_tag="PLB33_05810"
     CDS             8618..10816
                     /gene="infB"
                     /locus_tag="PLB33_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOG63072.1"
                     /translation="MRIYELAKELEMNSKDLAQLVEQELGITVKNHMSSLTDLEVLRF
                     KKALSLLNNSKKTKLVKSSKNVIVDEEPLVDEKIDLNQAKLKKVDKKLLSPVKKHTPT
                     KIGPAKLHHDEEEKKEIRKDIKKDKQKQKPQQQQQSRPEPKQHKVKNISIEDSIVVRD
                     FADKLGVPVNVVISKLIMLGVMANMNQEIDYDTASLIGEELGAKVNKIEVVDELKNLE
                     DSLFKDDKDDESNLQPRPPIVTVMGHVDHGKTSLLDAIRNTSVTEHEAGGITQHIGAS
                     QVEINGKKITFLDTPGHEAFTAMRARGAKVTDIAIIVVAADDGVMPQTIEAISHSKAA
                     GVPIIVAVNKIDKPTANPDKVKQELSEHGVISEDWGGDTIFVHVSAKKGTGIEELLEM
                     ILLVAEVQELKANPNRNAKGTIIESKLDKGRGPVATVLIEKGTLKKGDYVLVGSTHGK
                     VRAMFNSKGKRINQAGPSVPVEILGLSETPQAGDILVVMENEKDAKNIAEKRKDKKHL
                     ESINVSSKVSLDDLYERIQKGEVKDLNIIIKADVSGSIEAVKQSLQKLSVDEVKVNPI
                     HGGVGGINENDILLASASNAIVIGFNVRPSVAALELAKQENVDVRTYNIIYNAIEDIE
                     SAVKGMLKPVFKEVVMGRAEVRQTFKVPNVGIVAGVYVTSGKITRKSRIRLLRNDIVI
                     HDGQIASLKRFKDDVSELSSGYEGGIGLDKYNDIKIGDSMEAYIMEEIKR"
     gene            10830..11207
                     /gene="rbfA"
                     /locus_tag="PLB33_05815"
     CDS             10830..11207
                     /gene="rbfA"
                     /locus_tag="PLB33_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOG63073.1"
                     /translation="MGNRRNNRLSGEVRKAISEIIINDVKDPRISELMSITDVHVTED
                     LKYAKVFVSDYNNIETTLTALESAKGFIRKELGKKVKMRIIPELIFIKDDSIERGLHM
                     SSLIDKVIEEDKTRKVNKDNAEE"
     gene            11209..12156
                     /locus_tag="PLB33_05820"
     CDS             11209..12156
                     /locus_tag="PLB33_05820"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOG63074.1"
                     /translation="MKEIFNLINNSDKICIAGHKAPDGDCIGSVMALYNFLKPLNKNI
                     TVLMDGNIPYNYKAFIDEKIISNSYNNEEYDLLIVLDTSDRKRLGKFENVLNNSKKTV
                     VIDHHKTNEGFGNINIIDTEMSSTGELVYDILKAADADISLEVAALIYLAILTDTGKF
                     SYRNTTSNTHRKAAELIDIGVNVSEIDNIVYNSKPSNIVRAFMECVSGVELYYDNKLG
                     ITAITKNILNKHNADMADIDGVVEYIREIKEVEVSCVLKENDEGTKVSLRSKNNIDVA
                     AVSVKYKGGGHAKAAGFDINENIESTKKIIIEEFKKYFA"
     gene            12156..13025
                     /gene="truB"
                     /locus_tag="PLB33_05825"
     CDS             12156..13025
                     /gene="truB"
                     /locus_tag="PLB33_05825"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOG63075.1"
                     /translation="MIGILNILKPTGMTSHDVVSNVRKILNIKKVGHAGTLDPNVAGV
                     LVICVGKATKFSEYLMSGDKEYVCELILGQSTDTQDSYGMVIDKSDKVSVTREELNEV
                     LKKFTGELIQIPPAYSAVKLHGKKLYEYARQNIQVEKQGRPINIKSIELLKFDGNKAL
                     LKVRCSKGTYIRTLCNDIGKSLGTFGHMGILIRTESKGLHINNSVTLEKLKYLNDSNK
                     LDECLIPVESIYKLDKINVESKYYDRLTAGNEVEAGLNTDSDKFYVYCRNSLIGIGKK
                     VKDNTIKIDKMLR"
     gene            13038..>13789
                     /locus_tag="PLB33_05830"
     CDS             13038..>13789
                     /locus_tag="PLB33_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOG63076.1"
                     /translation="MIKTIGSCIAIGNFDGIHVGHDVLIRRMIDLSKERNLKSIIITF
                     KYVRNDLKKSSMNLKYINSPETKLEILKSYNVDEVIEIELNEIISKYSPEQFIKLLVD
                     DYNAKNIVVGYNFTFGHKASGNINTLKEFQDKYGYRVEEIPPVKYNGIAVSSSLVRTL
                     LNEGKINEANTLLLNKYTIYNDELNINYNKNIGFVDNKSSILVPPDGKYIVKLGSKEY
                     ILNIVSNKSGAALIFDKEVEENKDIVFINKAS"
BASE COUNT         5309 a         1872 c         2750 g         3758 t
ORIGIN      
        1 aaaatgacca ttgacgccta caaatctatg gcgaaaaagg ttaattatcc tcttcatctt
       61 ggaattactc atgcaggaac gttatttgaa ggcagcatac gctccgccat tggtgttgga
      121 agtcttttgg ctgatggtat tggcgacaca ataagaatat ctctaacaga tgacccgagg
      181 gaagaagtaa aggttgctag agaaatttta aaaagtctta atttaagaaa tttcggaata
      241 aattatataa catgtccaac atgcggtcga actaaaatcc agcttattga gctatccaaa
      301 aaaattcagg aaggtttaaa gaatgttaat aaacctataa cagttgcaat aatgggctgc
      361 gcagttaacg gaccgggaga ggcaagacag gcggatatcg gtatagcagg aggtaaagga
      421 gaggccctgt tgtttaaaaa gggggaaata atagaaaagg taaaagaaga agatttagtt
      481 aaaaggctgc tggaagaaat tgataaactc tcctaaggtt gacattcaaa caaaaggtgt
      541 agtataattt atatatgaaa attcaatatt ttttaattaa ccttatcgct gtataaacca
      601 aagcgtatta aataaattgc atatctaagg aggaaacatg gcagtaggaa ctttaaatgt
      661 agaaaagaga ttagaaacaa acagcagagc aagcaacaga ttgcgaagac aaggatattt
      721 gcccggaaac atatccagaa aaggtaagga ttcggtttca gttaccgtta aagctgatga
      781 actgagaaaa ggcttatcaa cttacggaag gaatgctttg tttaaaataa cattggatga
      841 cacggaattc accggtatgg tgaaagatat tcaactttca cctgttaagg gctcaatgat
      901 gcatgttgac tttcaagaag tatcattaaa tgaagaaatc aaagttcaat tatcaattgt
      961 gcttaaaggt acagaagcat tagaatttaa aggattaata gcattaacac aactagattc
     1021 aatcacagtg aaaggtttgc cgcagaatat ccctgatgac ctaacaattg atgtttcaaa
     1081 tatcgaaaaa gtagaaaata tatgtttaaa agatgtaaaa tttactgaag gaatagttcc
     1141 tgaaggtcac cctgaacaag ttttaatctc tattgttgaa gctaagagaa cagtagagga
     1201 agaagaaacc ggcgaagaaa ccgatgatga agtagcaggt gaataacaag attaataata
     1261 gtattaaata aaaataccca atttacgggt atttttttta caattcttct atatgaacaa
     1321 gtccttcaaa ggagtttagc ttttgtatta catcaacgtg attcattctt tcttttgctt
     1381 ccagtgtaat tattgcaata accgatattt cgtggggtat agtgtctttt gtaatttcaa
     1441 tgttgcgtac tttgaaatcg gataaattgc aaaaaattat gaatttatcc aattgctcaa
     1501 tggatttaaa cgaagtgtat atattgataa ttctattgtt tgatgttagc atataatcaa
     1561 tttttttaaa aaaggttatt ataataaaaa caatcaatgc tacaattact gcacctgaat
     1621 aaaaccctac acctattgca aggcctaagc atgcagatgc ccataaacct gcagctgaag
     1681 taagtccttt tatttggttt ctgcttgtta tcattattgt tcctgcaccc aaaaaaccga
     1741 taccgctgat aacctgtgca ccaagtctag cagggtcact gccgctgaaa ttattgtaaa
     1801 tatattgatt tgtcatcatt accaaagcag agcttaaaca tacaatcata taagttctga
     1861 aacctgcagg tctgtttttc ctgcttcttt caagtcctat aatacctccg caaatcatag
     1921 aaagtacaat tcgcaatgtt atggaatata tattgagttc atatagcgtt tcgattggaa
     1981 ttcctccttt gtatttatta atatattaat aaatgtaaaa tttattacat atgtttactt
     2041 ttaaaatata ttttaagtat attgaattaa aattttaatt ttgttatact acaataggag
     2101 aaaataaagg gagactgcta tgttatttaa taaaatattt actgatgaga gccttgataa
     2161 gataaaaatc caaaagattg aagtaataga aagaacaaat actattaatt tacacgcgtt
     2221 aagccaagaa attatagatt ttaatcatat agaaaatatt aaggacgaaa taagtaagca
     2281 ttataacaat tcagtaaatg tagaaatata tttaaaatat aaattagaaa aaaatataaa
     2341 cattgaaaaa attaaagaaa acctgcttta catcatcaat aaaagttcaa atatactgtc
     2401 aaaagcaaag gacaatataa aaattgaatt taaagatgaa tgtttttttg taattgcaga
     2461 caataaactg ctgatagacg agcttaatga caataacaca gaaagaaagc ttaagaaact
     2521 tatatgcgat tacaatttag actataatat aaaagctata tataatgagg cggaagaaga
     2581 agcagcaatt actgcacaaa gaatttttga ccaagcattg aaaaagaatt tagaaataat
     2641 taaaaataat tcttccgaaa aaaaacctgc caacaaatct caagaaggca gttataagaa
     2701 aaggtcaaga aaaaagattg actttgacag catacctgtg tctttaattt ccgaattaac
     2761 aaatgactca ggtgtagtta atattaaagg aaaaattgta aatgttgaca aaagggagtt
     2821 aaaaaacggc tctttcctca tatcgttaat cataaccgat tccacggatt caataatgtg
     2881 caaatatttt tgtcctaatg ggtttgatga tgaaggaatt gaaaatggga attatattca
     2941 aattgcaggc aatgttgaat atgatgatta tgaaaaagaa ttgattgtta aaatactaga
     3001 tttacagtta acaaccgaag aaatcaagcg aatggataat gcaaaagaaa agagggttga
     3061 gcttcatctg catacaggta tgagctcaat ggatggtata aacagcttta aggaatacgc
     3121 taagaaggca aggcagtggg gtcataaggc aattgcaata acagaccacg gtgttgtgca
     3181 aggctatcct gaagcgatgg attgtgccga tgataatttt aaagtcatat acggtatgga
     3241 aggatacctt gtaaatgaca ctgtctctat agttcaaaat tgtcaaaatg ttaatttgga
     3301 ttctgtattt gttgtatttg atatagaaac tacaggttta aatccaaaaa acgacaacat
     3361 catagaaatc ggagcagtga aaatttcaaa cagaaaggtt atcgacagct tttcaacatt
     3421 tgtacacatt gataaaaagc ttcctggaaa aatcattgaa ttaacatcaa taacagatga
     3481 tatgcttcaa ggtcaacccg aaatagatga ggctttgccg gcatttttgg attttgcaaa
     3541 agaatgtgta ttggttgctc acaatgctaa atttgacctt ggttttatta aagaaaacac
     3601 taaggtactg tctatagaaa attataatcc tccggtcttg gatactttgg agctctcaaa
     3661 agctctgata aaggatgtta aaaaccatag acttaatact ttaacgaaaa aattaggcat
     3721 aaatcttata aaccaccaca gagcagttga tgatgcaaat gcaacgggac agcttttgat
     3781 aattttattg aacaagttaa gtgaaagaga aatatatgat gtttctaaat taaatgatat
     3841 ccttatggaa gacatcgata tcgttaaaca gccgtcatat catatcaata tacttgttac
     3901 caactacata ggacttaaaa atttatacag tttggtttca gattcacata taaataattt
     3961 ttacaagaaa ccaagactgt taaaatctaa cttaaaccaa ttaagagaag gtctattgct
     4021 cggaactgct tgtgaatcag gagaactttt cagtgccttt cttgaaggca agagtgatga
     4081 aaagattgaa gaaatagctg attattatga tttttatgaa atacagccgc ttgacaacaa
     4141 tatgttttta gtaaacaatg aaagattaag aagtgttgaa gagcttaaaa taatcaacaa
     4201 aaaaattgtt gagttaggtg aaaggaaagg caagacagtt gttgcaaccg gtgatgttca
     4261 ctttttggag cctgaagatg aaattttcag aagaattctt atgtcaggac aaggctatga
     4321 ggatgcggaa gaacaagccc ctctatattt taaaacaacc gatgaaatgc ttgatgattt
     4381 ttcatattta ggtgaagaaa aggcatacga agtggttgta acaaacacaa atttaatcgc
     4441 agatttaatc gagcccatac ttcccattcc aaatggaaca tttcctccaa taatggaagg
     4501 ctcggacaat gagttaaaaa atatatgttt tagcaaagct aaatcaatat acggagaaaa
     4561 actccccgaa attgttgaaa gcagattaaa cagggagtta aattcaataa tcggtaacgg
     4621 atactcggtt atgtatatga ttgctcaaaa attggttaag aaatcaaatg aggacggcta
     4681 cttggtaggc tcaagaggct cggtaggctc ctcctttgca gcaacgatgg caggaataac
     4741 ggaagtaaac cccttagttc ctcattatgt atgcaacaaa tgcaagtatt ccgagtttat
     4801 agaagacgga agctacggct ccggttttga tttacctgac aagcattgcc ccaattgcgg
     4861 cactttattg gtaaaagacg gtcatgacat accctttgaa gtattcttag gatttgaagg
     4921 agataaagag cctgatatag atttaaattt tgccggtgag gaacaaggta tatgcatgaa
     4981 atataccgaa gagctttttg gagaaggaaa agttttcaga gcaggcacca taagtacaat
     5041 tgcagacaaa acagcctacg gctttgtgaa gaattatttt gaagaaagag gcattactaa
     5101 gagaaaagca gaaattgaaa ggttaattca aggatgtctg ggtattaagc gaacttcagg
     5161 acagcatccc ggcggggtta tgattgttcc gaaggataag gatattcatg attttacgcc
     5221 tgttcaatat ccagcaaaca atgaaaagtc gggaacgata acaacacatt ttgactacca
     5281 ttccatttcg ggacgaattt taaagcttga tttacttggt catgatactc caagcattat
     5341 acgcatgctg gaggatttaa caggactgga aagcaaagat attcctatgg atgacccaag
     5401 cacaatggcc atttttaaca gtcctgaaat acttggaatt tcattggatg aaataaattg
     5461 cagcacggga acattggcaa tacctgaatt cggtacttct ttcgtaaggc agatgattaa
     5521 ggatacaaag ccaaaatcat tttctgactt ggtcagaatc agcggtcttt cacacgggac
     5581 ggatgtctgg attaacaatg cgcaggatat aataaaaaaa ggttatgcga aaatcggggg
     5641 agttatatct actcgtgacg atataatgac atatttaatt caaatgggag tagaaaagaa
     5701 gacggctttt gatataatgg aaagagttcg aaaaggaaaa ggacttcgaa aagaagatga
     5761 ggaggcgatg aaggcacaga atgttcctca gtggtacata gactcctgca atacaattaa
     5821 atatatgttt cccaaggcac atgcggttgc ttatgtaacc atgtcagtaa aaattgctta
     5881 ctataaagtt cattatccgg aagcttttta tgcaacatat ttcaccacca aggttgatga
     5941 ctttgatgca gatattgtaa tgaaaggtca aaaccatata ttgaatacaa ttaaatcttt
     6001 tgaagaaaaa ggaaacgata agacagcaaa ggaaaaaaat ttaattactg ttcttgaagt
     6061 agcatatgaa atgtacaagc gaggtttcaa atttacaaaa gttgatttat ataaatccga
     6121 cgacagaaaa tttcttctga ccgaggacgg tatactgcca ccattgatgg gtcttcaagg
     6181 gcttggggca aatgcggcgg caaacataaa ggctgagaga gaaatttccg aatttatttc
     6241 aattgaagat attataaata gagcaaaagt atcaaagact gtaatagagg ttttggaaaa
     6301 acacggctgc ctgaaaaata tggatgaaac aaaccagatt tctattttta atgtgtgatt
     6361 gatttgtcct gtctttataa ctgagtgctc aataattcaa gtaatgtgtg tttttcgttg
     6421 tatgtatttc catacataag gtgcaccttt ttcatagata ttttcaaaca atacttgaaa
     6481 ttttgagcgg agcgagcaaa aaagcactat ttttcattgt gctatttata aaataatgtt
     6541 tgattttata aaatatttgt gatatactat taaaaagaag aattttgaca gaaagagtgg
     6601 gtgaccccac tctttctgat tgcagggtaa aggagtaaat atgaacaaga aagatatcgt
     6661 atcttcagtc agaaaaatta cagaagaaat actaaaatct acggatattg aattatttga
     6721 tatcgagtat gttaaagagg ggccctttaa gtatttaaaa gtctacatag ataagcccgg
     6781 gggaattact gtggatgata ccgctgatat aagcagagta ctcaataaga agcttgatga
     6841 ggttgattta atcaaggagc aatatttttt ggaagtttct tcaccgggag tggaaagacc
     6901 tttcaaaaca gaaaaagact atttgaaaaa cttaaataaa ttagtggaag caagattata
     6961 taaaccaatt gatgacaaga agactgtaaa aggcatatta acggaaaaga acgagaataa
     7021 tattgtaatc aaatcaggag aaaataattt tacgattgaa ctgaaagatt tagcaaaaat
     7081 taatagagtg atagaggatt tttaatggag gcgggttatg aacaaggata ttttaaatgc
     7141 attgagcgag cttgaaagag aaaaaggtat tttgaaagac gttgttttgg aagctataag
     7201 gtctgctttg gaaacaggat ataagaagaa ttacggtaag gcaacaaact tcagcattga
     7261 aatagataaa acaacaggtg aatataaact ttttcaggac aaaaccgttg tagaaaaagt
     7321 agaaaataaa aatttggaaa tttcattgga agaggcaaaa tcaatcgatt caaaatatga
     7381 attgggagat atagtcaaaa tacaaattga gcccaaagag gatttcggtc gaattgcagc
     7441 tcaaacagca agacaggtaa ttttgcaaaa aatccgcgaa gctgaaagag aatctatttt
     7501 cagtgaattt tacggcaggg aatcagatat tttgacagga atagtacaaa gagaaaacaa
     7561 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcaattg caagagaagc
     7681 cggctcaaga acaaaaatag cagttttttc ccatgacccc aatgtagagc ccataggctc
     7741 ctgtgtagga aataaaggta caagggttaa aaatattatt gatgaattaa atcaggaaaa
     7801 aattgatatt attatttatg aaaaagatcc cgaaaaattt attgccaata gtctgggacc
     7861 tgccaaaatt cttaaagtta cagcaaatga aaaggaaaaa acggcagtga ctgttgttcc
     7921 cgacaatcaa ctttctttgg caattggaaa ggaaggacaa aatgtaaggc ttgctgcaaa
     7981 gcttacaggg tggaaaattg atataataag cgaatctcag tataacgaca gcttacggga
     8041 ggaagtatga aagtcaggaa aataccaatg agaagatgta tcggctgcat ggaaagtttc
     8101 cccaaaaaag aattatgcag aattgtaaaa acaaaagaaa atgaaatttt tttcgatgaa
     8161 acaggaaaga agaacggacg aggaacttat atatgcaaaa atatagaatg cttcaataag
     8221 gcaattaaat caaagaggtt ggcaaaatca ttggaaaccg aaattcctga agaagtgata
     8281 gaagcaataa aagaggcgat tgaaaaaaat gcataaaata tactcaatgt taggtattgc
     8341 aagaaaagga ggaaatgtat ccataggttt tgattcaacg aaggcggata tagaaaaaaa
     8401 caatagtttt ttggttataa ttgcagaaga tgcatcagat aaaactaaga aaaacattaa
     8461 attcgtatgt gataagtata attgcgaata tattgagacg ggtgaaaaag aactattggg
     8521 aaaggctctt ggaaaaaagg tggtgagtgt tctgtcaata agagacagga acatagcatc
     8581 gtatttatta aataatgtat aaaccggagg tggttgcatg agaatatatg aacttgcaaa
     8641 agaattagaa atgaacagca aagatcttgc acaattagtt gaacaggaat taggaattac
     8701 agtgaaaaat cacatgagct ccctgactga tttagaggtt ttaagattta aaaaggcatt
     8761 aagccttctt aataacagta aaaaaacaaa gcttgtcaaa agcagcaaaa atgttatcgt
     8821 agatgaagag cccttggttg acgaaaaaat agatttgaat caagcaaagc ttaaaaaggt
     8881 tgataaaaaa ttgttatcac cggttaaaaa gcatactccg actaagattg gacctgcaaa
     8941 attacatcat gatgaagaag agaaaaaaga gattaggaag gatataaaaa aggataagca
     9001 aaaacagaaa ccacagcagc agcagcaaag ccgacctgag ccgaaacaac acaaggttaa
     9061 aaatatcagc attgaggatt ctattgtagt aagagatttt gcagacaagt tgggtgttcc
     9121 cgtaaatgtt gtaatatcca agttgattat gctcggtgta atggcaaata tgaatcagga
     9181 aatcgactat gacaccgcat cattgattgg agaagaatta ggcgcaaaag taaataaaat
     9241 agaagttgtt gatgagctta aaaatttaga ggattcatta tttaaagatg ataaagatga
     9301 tgaaagtaac ttgcagccga gaccacctat agttactgta atgggtcatg ttgaccatgg
     9361 aaaaacttcg cttcttgatg caatcaggaa tacatctgtt acagagcatg aagccggagg
     9421 aataactcag catataggag cgtcacaggt tgaaattaac ggcaaaaaaa taacattcct
     9481 tgatactccc ggacatgaag catttaccgc aatgagggca aggggagcaa aagtcactga
     9541 tatagcgata attgtagtag ctgcagatga cggtgtaatg cctcaaacaa ttgaagcaat
     9601 aagccattca aaagctgcag gagttcctat aattgtagcc gtaaataaaa tcgataaacc
     9661 tactgcaaac ccggataaag taaagcagga gctgtcagag cacggtgtta tatccgagga
     9721 ttggggcgga gatactatat ttgtgcatgt ttcggcaaaa aaaggcaccg gtatagaaga
     9781 actgttggaa atgatactat tggttgcaga agttcaggaa ttaaaagcta atccaaacag
     9841 aaacgcaaag ggtacaatta ttgaatcaaa gcttgataaa ggaagaggtc ctgttgctac
     9901 cgtattgatt gaaaagggaa cattgaaaaa aggcgattat gtattggtag gaagcactca
     9961 tggcaaggtc agagccatgt tcaattcaaa aggcaaaaga attaatcagg cgggaccgtc
    10021 tgttccggtt gaaatattag gactttctga aacgcctcaa gcgggagata tattggtagt
    10081 aatggaaaat gaaaaggatg ctaaaaatat tgcagaaaaa cgcaaggata aaaagcacct
    10141 cgaaagtatc aatgtttcat caaaggtgtc actcgatgac ttatatgaaa gaattcaaaa
    10201 gggtgaagta aaagatttaa atatcattat aaaagcggat gtaagcggct caatagaagc
    10261 agttaaacaa tcattgcaaa aattgagcgt ggacgaagta aaagttaatc ctatacacgg
    10321 tggtgtgggt ggaataaacg aaaatgatat tttgcttgct tctgcttcaa atgcaatagt
    10381 aataggattt aacgtaagac cttcggttgc agctcttgag cttgcaaaac aggaaaatgt
    10441 ggatgtacgt acttacaata ttatttacaa tgcaattgaa gatattgagt ctgctgttaa
    10501 gggaatgctt aaacctgtat tcaaggaagt agttatggga agagctgaag taagacagac
    10561 attcaaggtg ccaaatgtgg ggattgttgc cggggtttat gtaacaagcg gaaagattac
    10621 aagaaaatcg agaataagat tgctgaggaa tgatatagta atacatgacg gacaaattgc
    10681 atcgctaaaa aggtttaaag atgatgtgtc tgaacttagc agcggttatg aaggcggaat
    10741 aggtcttgac aaatataatg acattaaaat aggtgattca atggaagcct atataatgga
    10801 agaaataaaa agataaaaag aggtaagaaa tgggtaatag aagaaacaac aggttatcgg
    10861 gcgaagttag aaaagcaata tccgaaataa tcataaatga tgtaaaggac ccgagaattt
    10921 cagagcttat gagtataacg gatgtacatg taacagaaga tttgaaatat gcaaaagtat
    10981 tcgtttcaga ctataacaat attgaaacta ctttaactgc tttggaaagt gccaagggtt
    11041 ttataagaaa agaactgggt aagaaagtta aaatgagaat tattcccgag ctgatattta
    11101 tcaaagatga ttcaattgaa agaggcttgc acatgagcag tcttatcgac aaagttattg
    11161 aagaagataa aacaagaaag gttaataaag ataatgctga agaatgatgt gaaggaaata
    11221 tttaatttaa taaataattc tgacaaaatt tgtatagcag gacataaggc gcccgatgga
    11281 gactgcatag ggtcagttat ggccctttat aactttctta aaccattaaa taaaaacatc
    11341 acggtattga tggacggcaa tattccatat aattataaag cctttataga tgaaaaaata
    11401 atttcaaaca gctataacaa tgaagaatat gaccttttga tcgtattgga cacaagtgac
    11461 agaaaaaggc tgggcaaatt tgaaaatgtg ttaaacaact caaagaaaac agtagttatt
    11521 gaccaccaca aaaccaatga aggcttcggt aatataaata taatagatac agaaatgagc
    11581 tccacgggag aattggtata tgatatatta aaagctgcag atgctgatat ttctcttgaa
    11641 gttgctgctt taatctacct tgccatatta accgacacag gaaagttttc ttatagaaac
    11701 actaccagca atacacacag gaaagctgca gagcttattg atatcggtgt taatgtatcg
    11761 gaaatagaca atattgttta taattccaaa ccctccaata tagtaagagc atttatggag
    11821 tgcgtaagcg gggttgagct ttattatgac aacaaattag gcataacggc tataacaaaa
    11881 aatatactta ataaacacaa tgccgatatg gctgatattg acggagttgt tgaatatatc
    11941 agagaaataa aagaagttga agtatcctgc gtacttaaag aaaacgatga aggtacaaag
    12001 gtgagtctaa ggtcaaaaaa caatatagat gttgctgctg tttcagttaa atacaaaggc
    12061 ggcggtcatg caaaggccgc aggatttgat attaatgaaa acatagaaag tacaaaaaaa
    12121 ataataattg aggaatttaa aaagtatttt gcgtaatgat aggaatatta aatatattaa
    12181 aaccaacagg catgacatcc catgatgttg taagtaatgt cagaaaaata ttaaatataa
    12241 aaaaggtagg ccatgcagga actcttgacc ctaatgtggc aggagtattg gttatttgtg
    12301 tcggtaaagc aacaaaattc agcgaatact taatgagcgg cgacaaagaa tatgtatgtg
    12361 agcttatatt gggacaaagc accgatactc aggacagcta cgggatggtt attgataaat
    12421 ctgataaagt atcagtaaca agagaagagt taaatgaggt tttaaaaaaa tttacgggag
    12481 agctaattca aattcctcct gcatattcag cagttaaact acatggtaaa aaactgtatg
    12541 aatatgcaag gcaaaatata caagtagaga aacaaggaag accgataaat attaaaagta
    12601 ttgaacttct taaatttgac gggaataaag ctcttttaaa agttagatgc agcaagggaa
    12661 catatattag aactttatgc aatgatatag gaaagagctt gggtacattc ggacacatgg
    12721 gaattttaat cagaacagag tcaaaaggtt tacatattaa taattctgtg acccttgaaa
    12781 agttgaaata tttaaatgac agtaataagc tggatgagtg ccttattccg gttgaaagta
    12841 tttataaatt agataaaata aatgttgaat cgaaatatta tgacaggctg actgcaggaa
    12901 atgaagtaga agcaggatta aatacagaca gcgataaatt ctatgtatac tgtcgaaaca
    12961 gtctgatagg tattggaaaa aaagttaaag ataatacaat aaaaattgac aaaatgctta
    13021 gataacgggt gtgacatatg ataaaaacaa taggttcatg tattgcaata ggtaactttg
    13081 acggcataca tgtgggacat gacgttctaa tcagaagaat gattgattta agtaaagaaa
    13141 gaaatctaaa aagtataata ataacattta aatatgtaag aaatgacctt aaaaagtctt
    13201 ccatgaattt gaaatacata aacagtccgg aaacaaaatt ggaaatactg aaatcttaca
    13261 atgtagatga agttattgaa attgaattaa atgaaattat atcaaaatat tcaccagagc
    13321 agtttattaa gcttctggtg gatgattata atgcaaaaaa tattgtagta ggctataatt
    13381 ttactttcgg gcacaaagca agcggcaata taaatacctt gaaagaattt caagataagt
    13441 acggatatag agtggaggaa atacctcccg taaaatataa cggtattgca gtaagttcat
    13501 cattagtaag aaccctatta aatgaaggta aaattaatga ggctaataca cttttattaa
    13561 ataaatatac catctataat gatgagttaa atattaacta taataaaaac ataggttttg
    13621 tagataataa aagcagcatt ttagttcctc ccgacgggaa atatatagta aaattaggga
    13681 gtaaagaata tattctaaac attgtttcta acaaaagcgg agctgctttg atatttgata
    13741 aagaagttga agaaaacaaa gatattgtgt tcataaataa agcttctta
//
LOCUS       DAOSHP010000046        13734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3715_length_13734_cov_9.031581, whole genome shotgun sequence.
ACCESSION   DAOSHP010000046 DAOSHP010000000
VERSION     DAOSHP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13734
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3715_length_13734_cov_9.031581"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..299)
                     /locus_tag="PLB33_05835"
     CDS             complement(105..299)
                     /locus_tag="PLB33_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63077.1"
                     /translation="MNELEKIKKMYDNGFRCIRYDDTKDGDMCIYFKNFDNEESEALR
                     VSDFEQKMQIKSFIKENSMK"
     gene            complement(401..1165)
                     /locus_tag="PLB33_05840"
     CDS             complement(401..1165)
                     /locus_tag="PLB33_05840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOG63078.1"
                     /translation="MIKGILFDKDGTLIDFSLWRNAGINTIQTILNEYNLNNNKLNIE
                     LQRAIGIKEKGVEPFGALAYKSHEDVAYELHCILNKYINIDFDKFETHVVGLLRNEVL
                     RDDVEFKEIVSIRKLYEHLNSNNIKMGLATADSMQSAMHMINKLNLNDSFDFIGSYDG
                     KMKRKPHKDMCMKFCSMYNLEPGEVAIVGDSYSDMLFALNSGAIGVGVLSGVSSKINL
                     KDVANVIVPSVESLFDRNVLEALDEKSYEARELRTA"
     gene            complement(1504..1794)
                     /gene="yhbY"
                     /locus_tag="PLB33_05845"
     CDS             complement(1504..1794)
                     /gene="yhbY"
                     /locus_tag="PLB33_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOG63079.1"
                     /translation="MLTGKQRSYLKALANSVDTIMQIGKGGITDSVLKQIDDALNARE
                     LIKVSVLKNSMLDAKSVANEVAESLGAEYVQSIGGKFVLYRESKEKKINIPK"
     gene            complement(1806..3080)
                     /gene="obgE"
                     /locus_tag="PLB33_05850"
     CDS             complement(1806..3080)
                     /gene="obgE"
                     /locus_tag="PLB33_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOG63080.1"
                     /translation="MFVDIAKISVKAGKGGNGSVAFRREKYIPMGGPAGGDGGDGGSV
                     ILVADEGLRTLMDFRYKKHYHAENGEDGRGKKQYGSAGEDLYLRVPVGTLVKDAETGI
                     VLADLKTHGQQYVAARGGRGGKGNVKFKSSIRRTPRFAQPGTKGDEREIILELKLLAD
                     VGLIGFPNVGKSTILAAITSAKPKIANYHFTTLKPNLGVVSIGEGHSYVLADIPGLIE
                     GASEGAGLGLDFLRHIERTKLLLHVIDASGQEGRDPVEDFHKINDELKQYSNKLSGKE
                     QIIVLNKMELPDAINNAERIKKEFSEKYEIIQVSAATGSGLDFLKKRAYERLLEIEEE
                     IEFVDEEAVESFLDKKERDTILVTKEDGYYSAEGDFLERLVASTNFDDFESFSNFQKV
                     LIDKGVIQKLKELGAEEGDLISVCGIEFDFVD"
     gene            complement(3172..3465)
                     /gene="rpmA"
                     /locus_tag="PLB33_05855"
     CDS             complement(3172..3465)
                     /gene="rpmA"
                     /locus_tag="PLB33_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOG63081.1"
                     /translation="MLLKLNLQLFAHKKGVGSSRNGRDSNSKRLGVKRTDGQFVLAGN
                     ILVRQRGTKLHPGVNVGRGSDDTLFAMIDGKVKYERKDKTRKQVSVYPVELDA"
     gene            complement(3468..3794)
                     /locus_tag="PLB33_05860"
     CDS             complement(3468..3794)
                     /locus_tag="PLB33_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOG63082.1"
                     /translation="MITVTIYRTKDEFKGFIVEGHSDYAEEGLDIVCASVSILSYTAL
                     NSLNLVAGITPENIEYTVDDEGVMSLRTLENNNKTDIVYRTFMVGIELLLEDYSDYIT
                     LKFEEV"
     gene            complement(3796..4107)
                     /gene="rplU"
                     /locus_tag="PLB33_05865"
     CDS             complement(3796..4107)
                     /gene="rplU"
                     /locus_tag="PLB33_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOG63083.1"
                     /translation="MYAIIETGGKQYRVQEGDVLRVEKLDIAEGETVKFDKVLLVAEE
                     GKVNVGKPYVDGAVVEAVVENHGKAKKIIIFKYKSKKDYRKKQGHRQPYTQIKVEKIV
                     G"
     gene            complement(4339..>5772)
                     /locus_tag="PLB33_05870"
     CDS             complement(4339..>5772)
                     /locus_tag="PLB33_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOG63084.1"
                     /translation="KLIDYLFELNNNQKVVGNIYKGKVINILPGMQAAFVDIGLNKNA
                     YLFLDDLLSDKFLKELKIKKKDVSNISKVLKTGDELLVQVVREPMGEKNISVTTNISL
                     TGKFIAFIPNNNVVNLSKKIKDPDERKRLEEIGKSIILNGNGMIIRTFAKGSHKEAIE
                     KEYKMLSNIYMKIEKEYKYSYAPKLLHKSNSLIDRLFLDYIDSSVEEIYVEDKITKDK
                     FNDMISGLPGDILKNIKIIQAQRVFENFNIASQINMLFERRVELDNGGFIIIDVTEAL
                     TVIDVNSGKYTGSRNMEETALAINLSAIEEIARQIKMRNLSGIILIDFIDVKKEESIN
                     LLIAKAKSLFRDDKTKTNVIGMTKLCIMEITRKRNTENFFNLITEECSHCSGSGRSIS
                     KMYIFIKIEGIIKNIKHNTSSEAVILNVGCILYNKIIKSCMDIIAEIEKKYDIKIFIE
                     QDDKILTDEIIIGKIGKLSNVNGIYQI"
     assembly_gap    5773..5872
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5873..6195)
                     /locus_tag="PLB33_05875"
     CDS             complement(<5873..6195)
                     /locus_tag="PLB33_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOG63085.1"
                     /translation="MCKIICKYSKTGYLKYISHLDVLRFIQRSVKRAGIKAKYSEGFN
                     PHMKTSFGYPLSLGIESIGEYFELELNENIEPELFKDKMNSVMPKEMQIIKADYSNDA
                     ESLMK"
     gene            complement(6176..8035)
                     /locus_tag="PLB33_05880"
     CDS             complement(6176..8035)
                     /locus_tag="PLB33_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOG63086.1"
                     /translation="MIDVKKLDRVLLSVEKPARYVGSELNSVVKNKDIDIRFAFAFPD
                     IYEVGMSHTGLHILYHLMNSMENVWCERVFAPWTDMEEVMRKNNIPLYGLESKDDLTA
                     FDFVGFTLQYEMSYTNVLNMLDLGNIPIKSKDRGNDMPLVIAGGPCVYNPEPLHEIID
                     LFVVGEAEEVLPELLNLYNEEKKNVYDKLKFLEKAASIEGIYIPQFYEEIYNNDGTIK
                     ERIVLNPNAKETIKKRIIKDLSKSYYPEKSILPYIDVVHDRVVLELFRGCTRGCRFCQ
                     AGMVYRPVRERSTDTLINQAERLLSSTGHDEVSLTSLSSGDYSCLPDLTIRLLDKYEN
                     QNISISLPSLRLDSMTFDIIERLQEVRKSGLTFAPEAGTQRMRDVINKNLTEEQILGP
                     VETAFKLGWSTVKLYFMIGLPGETMEDVLGIKELSYKIKDKFFHRPKDEIKGNLKINV
                     SAACFVPKPFTPFQWAAQNNMDEFYEKAKVLQREIKDKKISFSYHEPKLSYLEAVFAR
                     GDRRLSKALIKAWEKGCRFDGWYDLFDFDKWMSAFEETNIDPDFFALRQREVREILPW
                     DFIDIGVKKEYLVSEYEKALDFDTTPDCRQRCTNCGVNYNLWGGACPHVQNNM"
     gene            8404..9714
                     /locus_tag="PLB33_05885"
     CDS             8404..9714
                     /locus_tag="PLB33_05885"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOG63087.1"
                     /translation="MNYDEALQFIHSTYKFGSKLGLERIKRLMELLGNPQDCCKIIHV
                     AGTNGKGSTCNLIHDVLMESGYKTGLFISPYLEEFTERIQINKQKIDKDSLTRIAELV
                     RDKVNTMVSEGYDHPTEFEVVTAIGFKYFQEQNIDFLVLEVGMGGRFDATNVIKKPLL
                     SVITSISYDHMEQLGNTLEKIAFEKAGIIKENSTVIIYPQTINITNVIKDVAKEKNAK
                     VYEADKSNIEKTKSDITGQWFKYLKRDVFELPELKINLLGEHQLLNALTALSALEIIK
                     DKGYDISTKSIENGFSNCKHPGRFEIINKNPYVILDGGHNIDGIRSFTNTLKEYFKDK
                     VTFFFGILKDKSPEEALQLILPLAKEIYTLTPLSPRALKAADLAGLIKKYSDIKVIPL
                     ENYEDMLPIIKNAGKNEIIAFSGSLYMIGNVRTLLRNNGITNNL"
     gene            complement(9796..10146)
                     /locus_tag="PLB33_05890"
     CDS             complement(9796..10146)
                     /locus_tag="PLB33_05890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOG63088.1"
                     /translation="MIVKRGDVYYADLSPVIGSEQGGVRPVLVVQNDIGNRYSPTVIV
                     SAITSQINKAKLPTHIEISSEDFSLPKDSVVLLEQIRTIDKRRLKEKIGRFDKNLMGE
                     VDDCLKISLGLIDF"
     gene            complement(10148..10423)
                     /locus_tag="PLB33_05895"
     CDS             complement(10148..10423)
                     /locus_tag="PLB33_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOG63089.1"
                     /translation="MAENKKVLISIPANLLRELDNAVKEEGTNRSDFIRKSIRFYLIE
                     KRKLEIRNKMKAGYLEMSKINKSITEEYSEGDYEDFLSYETKLLGSE"
     gene            complement(10523..11020)
                     /locus_tag="PLB33_05900"
     CDS             complement(10523..11020)
                     /locus_tag="PLB33_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63090.1"
                     /translation="MRLIIFILVLLMALSGCTAEDKPFILPVNYSANALMKVYTDKTE
                     NDYKINIICRDGNYSIRTETENEAWNCAYVSGNRCILNNDKFPESSVTIENYKLNDTL
                     LHDFDFGKFDVFEDIPEELVYWDGEYKHVLNFSKDNMLPKTIFIYKYDKLVKAIQYEK
                     INIEE"
     gene            complement(11182..11547)
                     /gene="acpS"
                     /locus_tag="PLB33_05905"
     CDS             complement(11182..11547)
                     /gene="acpS"
                     /locus_tag="PLB33_05905"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOG63091.1"
                     /translation="MIKGTGIDITEVERIKKNMGNIRFLEKIYSNRELEYLKTRKFNP
                     QTAAGMFAAKEAVAKCLGTGFSNFGPCHIEILKDENDKPYVFLLSNALKRAEELNIKN
                     IYISISHTQEYAVAHCVAE"
     gene            11707..12336
                     /gene="upp"
                     /locus_tag="PLB33_05910"
     CDS             11707..12336
                     /gene="upp"
                     /locus_tag="PLB33_05910"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOG63092.1"
                     /translation="MNNITVVSHPLIQHKLTMLRDKNTPPKDFRELVREISMLLAYEV
                     TRSLPLKDIDIETPICKTTGKILSGADIAIVPVLRAGLGMVDGMLDLIPNAKIGHIGL
                     YRNEETLEPVEYYCKLPSDIDKRIVIITEPMLSTGGSMNGALNMLKKRGAKKIKSIHL
                     VCAPEGLKRVTETHPDVEIYTAAIDEKYNEIGYIVPGLGDAGDRLFGTK"
     assembly_gap    12443..12542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12760..13704
                     /locus_tag="PLB33_05915"
     CDS             12760..13704
                     /locus_tag="PLB33_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOG63093.1"
                     /translation="MKHIFIINPIAGNGKLQEKLEKTIHLELKDKEIDYEVYITKSKE
                     DTRNFAENKCKENIQAVLYSCGGDGTLHEIINGACRYKHVSIAIIPIGSGNDFIKNFK
                     NSANFSDINLQTEGESIELDLIKVNNEYSANVLNIGLDADAAYNMDKFRKFPFIQGTT
                     RYYLSMLYSLFGKLGKHIEVNTENGVINGTFLIGVIANGQYYGGGYKCAPKAKVNDGL
                     LDLCFVEKISRAKILNIIGSYKKGEHLDNPKINKYIIYDKINQANIKFDKQTNVCMDG
                     EIYTYNELNISTEKNALKFWIPKGVQLQTQQTENHSFV"
BASE COUNT         4168 a         2696 c         2023 g         4647 t
ORIGIN      
        1 agccaaagct tttgcttcat tgtttgcatt cattatatat tagaaggaat cgttttacaa
       61 gaaataattg gacataaata ggaactgtaa acttaagttc cctcttattt catggaattt
      121 tctttgataa aactttttat ctgcattttt tgttcaaaat ccgaaactct taaggcttct
      181 gattcttcgt tatcgaaatt tttgaaatat atgcacatat cgccgtcctt agtatcatca
      241 taccttatgc acctgaatcc attatcgtac atttttttaa tcttctctaa ttcgttcata
      301 atattcaccc cttatgtttt atacatatta ttgaccaaat aaaggaaatt atgactgaat
      361 taaagaaaaa aataagcctt attgcatttc ttgcacaggc ttatgctgtt cgaagctccc
      421 ttgcttcata acttttttca tctaaggctt ctagcacatt tctgtcaaac aaagattcaa
      481 cagaaggaac tataacgttg gctacatctt ttaaattaat tttactgctt actccggaca
      541 gtacccctac tccgattgca cctgaattta atgcaaagag catgtcgcta tatgaatccc
      601 ccactattgc aacctctccg ggctccaaat tatacatgct gcaaaatttc atgcacatat
      661 ccttgtgagg ttttcttttc attttaccgt cataggagcc tataaagtcg aagctgtcat
      721 ttaagttcag cttattaatc atatgcattg ctgattgcat ggaatctgcc gttgccaaac
      781 ccatttttat attgtttgaa ttcaaatgtt catacaattt tcttatgctg acaatttctt
      841 taaactcaac atcatccctt aaaacttcat ttctcaatag tccgaccaca tgagtttcaa
      901 atttgtcaaa gtctatattt atatacttat ttagtatgca atgaagctca taagccacat
      961 cttcatgcga tttataagcc aaggcaccaa aaggctccac tcccttttcc tttatgccga
     1021 tagctctttg cagctctata ttaagtttgt tattattcaa attatattca tttagtattg
     1081 tttgaattgt attgattccg gcatttctcc acaatgaaaa atctatcaag gtgccgtcct
     1141 tatcaaacag tattcctttt atcattttat cacctcctta tatttcatat tgtaaatacg
     1201 aaaataaata tgatatgctc atattgtaat acgtataaat aagtgcatct tttttatttt
     1261 ataatatgat tctttaaaca ttcttaaatt gcggggagta ctattatatt aagaaaaagc
     1321 tgaaatgtga tataaatttg aaattcaaac aaatatgata agaatcatca cgtattatct
     1381 acaccacgat aaatgaatgc gctttgtttt cccgagcgaa agatctcata agattcttca
     1441 tatgcgttca gaatgacact gacgaatcag gtgcatgaat gattgcattc cggcacctga
     1501 cttttactta ggtatattaa ttttcttttc tttagattct ctgtataaaa caaatttgcc
     1561 gcctatactt tgaacatatt cagcacctag agattctgca acttcatttg caacagactt
     1621 tgcatccagc atgctgtttt ttaatacact caccttgatt aattctctgg cattaagtgc
     1681 atcgtcaatt tgttttaata cgctgtcagt aataccgcct ttgcctatct gcattattgt
     1741 atcaacagaa tttgccaaag cttttaaata acttctttgt tttccggtaa gcatttactc
     1801 tcctttcaat ctacaaaatc aaattctatt ccgcatacgc tgattaagtc tccttcttca
     1861 gcgccgagtt cttttaattt ctgtattaca cccttgtcaa tcagtacctt ttgaaaattc
     1921 gaaaaagatt caaaatcatc aaaatttgtt gatgcaacaa gtctttcaag aaaatctcct
     1981 tctgctgaat aataaccgtc ttcttttgtt acaagaattg tatctctttc ttttttgtct
     2041 aagaaacttt caacagcttc ttcatcaaca aattcaattt cttcttcaat ctctaaaagt
     2101 ctttcatagg ctcttttctt aagaaagtca agtccgctgc ctgttgcggc agaaacttga
     2161 attatttcat atttttctga aaattctttt ttaattcttt cggcattatt tatagcatcc
     2221 ggcagctcca ttttgtttaa aacaattatt tgttcttttc cggatagttt attgctgtat
     2281 tgtttaagct catcatttat tttatggaaa tcttcaaccg ggtctcttcc ctcttgaccg
     2341 gaagcatcga taacatgcaa aagcagcttt gttctttcaa tatgccttaa aaaatcaagc
     2401 ccgagacctg caccttcaga agcaccttca ataagccccg gaatatctgc aagcacatag
     2461 ctgtgtcctt ctcctatgct cacaactcca agattcggtt ttaaagtcgt gaaatgataa
     2521 tttgctattt tcggtttagc gcttgttata gctgctaata ttgttgattt ccccacgttt
     2581 ggaaacccta ttaatccgac atcggcaaga agcttaagct caagaataat ttctctttca
     2641 tcgcccttgg ttccaggctg tgcaaaacgg ggagtacgcc ttatggaact tttaaattta
     2701 acatttcctt ttcctccgcg tccgcctctt gctgctacat attgctgtcc gtgggttttt
     2761 aagtctgcca gtactatgcc tgtttctgca tcttttacca aagttccgac aggaactctc
     2821 agataaaggt cctccccggc agaaccatat tgttttttcc ctcttccgtc ttcaccgttt
     2881 tccgcatgat aatgtttttt atatctgaaa tccatcaatg ttctaagccc ttcatcggca
     2941 accaaaatga cgcttccgcc gtcgcccccg tcgccgcctg caggtccgcc cattggtatg
     3001 tatttttcac gtctgaatgc tacgcttccg tttccgccct tacctgcttt tacacttatt
     3061 tttgctatat ctacaaacat ttttgttcct ttccgccact cttgtcatcc tgacaatctt
     3121 tatatttctt catctaaaaa tgacctaccg tatggtaggt cttttctttt attatgcatc
     3181 taattcaaca ggatatacac tgacctgttt tcttgtttta tcttttcttt cgtacttaac
     3241 ctttccgtca atcatggcaa atagcgtatc atcacttcct ctgcctacgt taactcccgg
     3301 atgaagttta gtacctcttt gtctaacaag aatattcccg gccaacacga attgaccgtc
     3361 ggttcttttt actcccaatc tcttagaatt gctgtccctg ccgtttcttg agctaccaac
     3421 acctttttta tgtgcaaata actgtaagtt taatttcaat aacattttta cacctcctcg
     3481 aatttaagtg taatataatc gctgtaatct tccaataata attcaattcc aaccatgaag
     3541 gttctgtaca ctatatcagt tttgttatta ttctctaatg tcctcaaact cattactccc
     3601 tcatcatcga cggtatattc aatgttttca ggggtaattc ccgccacaag gttcaatgaa
     3661 ttcaatgctg tgtaagacaa aatggaaact gatgcgcata ctatgtccaa tccttcttca
     3721 gcgtaatccg aatggccttc tactataaaa cctttgaatt catccttggt tctatatatg
     3781 gtaactgtaa tcatactatc ctactatctt ctcaacctta atttgagtat atggctgacg
     3841 atgaccttgc tttttcctgt agtctttttt agacttgtat ttgaaaatta ttattttttt
     3901 agctttgccg tggttttcaa ctactgcttc aactactgca ccgtcaacat atggtttgcc
     3961 tacattcact tttccttctt cggctacaag caatacctta tcaaatttta cggtttcacc
     4021 ttcagcgata tcaagttttt caactcttaa cacatcgcct tcttgaactc tatattgttt
     4081 tccacctgtt tcaataattg cgtacatatt tttacctcct cttcccagtc tcgccattgc
     4141 aggtacttag cgttttcaac ctgatccgtg cggctctaac agatattgat tgtatcaaac
     4201 tataaaaaaa ttgtcaactt attttttgat gacctaaccc catttttctg aaaaatggat
     4261 ggtaggtcag acgcagcaca ttcccatttt gcacgaccac agggagtgag caaaatggtg
     4321 atggagcctg cgaaactgct atatttgata tattccattt acattggaca actttcctat
     4381 tttcccgatg ataatttcat cggtaagaat tttatcatcc tgctcaataa atattttaat
     4441 atcgtatttt ttttcaattt cagcaataat atccatacag cttttgatta ttttattgta
     4501 caatatacat cccacattta aaataacagc ttcggaagat gtattatgct taatattctt
     4561 tattattcct tcaattttaa taaatatata catttttgaa atgcttctgc cgcttccgct
     4621 gcagtgtgag cactcttcgg ttatcagatt aaaaaagttt tccgtgtttc ttttccttgt
     4681 tatttccata atacatagct ttgtcattcc gataacattt gtctttgttt tatcatccct
     4741 aaacaatgac ttggctttag ctattagcag attaatactt tcttccttct taacatcaat
     4801 gaaatcaatt aaaataattc cgcttaaatt tctcatcttt atctgccttg caatttcttc
     4861 gattgcagat aggtttatag ccaaagctgt ttcttccata tttcggcttc cggtatattt
     4921 cccgctgttt acatcgatta ccgtcaatgc ttccgtaaca tcaattatta tgaagcctcc
     4981 gttatcaagc tcaactcttc tttcaaacag catatttatc tggcttgcaa tattgaaatt
     5041 ttcaaaaacc ctttgtgcct gtataatttt aatattttta agaatatctc cgggcaagcc
     5101 cgaaatcata tcattaaatt tatcttttgt aattttatct tcaacataga tttcttctac
     5161 acttgagtct atatagtcca aaaataacct gtcaatgaga gaattgcttt tatgcaaaag
     5221 cttcggtgca tatgaatatt tatattcctt ttcaatcttc atatatatat tggaaagcat
     5281 cttatattcc ttttctattg cttctttgtg actgcccttg gcgaatgttc ttattatcat
     5341 tccgtttccg tttaagatta tacttttgcc tatttcttca agtctttttc tctcatccgg
     5401 gtctttgatt ttctttgaaa gattaactac attattattg ggaataaatg ctataaattt
     5461 tcctgtcaat gaaatattgg ttgtaacaga aatatttttc tcccccattg gctctctcac
     5521 aacctgaacc aagagttcat cacctgtttt taacactttg ctgatatttg aaacgtcttt
     5581 tttcttaatt ttcagttctt ttaaaaactt atcggaaagc aggtcatcca aaaacaaata
     5641 tgcattttta ttaaggccta tgtcaacaaa ggcagcttgc atgccgggaa gaatattaat
     5701 aacctttcct ttatagatat tgcctacaac tttttgatta ttgtttaatt caaaaagata
     5761 atcaataagc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttttcat
     5881 aagagattcg gcatcattgg aataatcggc tttaattatc tgcatttcct ttggcatcac
     5941 agagttcatt ttgtctttaa ataactccgg ctcaatattt tcattaagct ctaattcaaa
     6001 atactcccca atactttcta ttcccagcga caagggatat ccaaacgatg ttttcatatg
     6061 aggattaaat ccttccgaat atttagcctt tatgcctgcc ctttttaccg acctttggat
     6121 aaatcttaaa acatccaagt gggaaatata ttttaaatat cctgtcttgc tgtatttaca
     6181 tattattttg cacatgaggg caagcacctc cccataaatt atagtttaca ccgcagtttg
     6241 tacacctttg cctgcagtca ggagtagtat caaagtctaa tgctttttca tattcgctga
     6301 ctaagtattc tttcttaact ccaatgtcta taaaatccca tggtaaaatt tctcttactt
     6361 ccctttgtct caatgcaaaa aaatcagggt caatgtttgt ttcttcaaat gcactcatcc
     6421 acttgtcaaa atcaaataaa tcgtaccacc cgtcaaagcg gcatcctttt tcccatgctt
     6481 taatcaaagc tttggataat cttctgtctc cccttgcaaa tacagcttcc aaataactta
     6541 atttaggttc atggtagctg aaggaaattt ttttatcttt aatttctctt tgcaatactt
     6601 ttgccttttc atagaattca tccatattgt tttgtgcagc ccactgaaag ggtgtaaaag
     6661 gttttggtac aaagcaggcg gcacttacat ttatttttaa atttcctttt atctcatcct
     6721 taggtctgtg gaaaaattta tccttgattt tataagaaag ctctttgatt ccgagcacat
     6781 cttccattgt ttcaccgggg agccctatca tgaaatacaa tttaaccgtt gaccacccta
     6841 atttaaaagc agtttcaacc ggtcccaaaa tttgttcttc cgtcagattc ttatttataa
     6901 catctctcat cctttgagtt cctgcttccg gtgcaaatgt aaggccggat ttacgaactt
     6961 cctgcagtct ttcgataata tcaaaggtca tagaatcaag cctcaacgaa ggcagagaaa
     7021 tacttatatt ttgattttcg tacttatcta acagccttat agttaaatcc ggtagacatg
     7081 aataatctcc ggagcttagg gaagttaatg atacttcatc atgacctgtt gagcttagaa
     7141 gtctctcggc ttgatttatc agcgtatcgg tacttctttc ccttacaggt ctgtaaacca
     7201 tcccggcctg acaaaaacgg cagcctctcg tacagccacg gaataattcc aaaactactc
     7261 tgtcatgaac cacatcaata tagggcagta tacttttttc aggataatag cttttgctta
     7321 aatccttaat aatacgtttc tttattgttt ctttggcatt tggatttaac actattcttt
     7381 ctttaatcgt accgtcgtta ttatatattt cttcataaaa ttgcggaata tatatacctt
     7441 ctattgatgc cgccttttca agaaatttaa gtttatcata gacatttttc ttttcttcgt
     7501 tatataaatt caacagttca ggcaaaacct cttcagcttc accaacaacg aataaatcta
     7561 ttatttcatg cagaggctcc ggattatata cacaaggacc tcctgcaatt accaagggca
     7621 tatcattacc tctgtccttt gattttatag gtatattacc taaatcaagc atgtttaaaa
     7681 catttgtgta gctcatttca tattggagag taaatccaac aaaatcaaag gcagtaaggt
     7741 catccttgct ttccaagcca tacaaaggaa tgttattttt cctcataact tcttccatat
     7801 ctgtccaagg tgcaaatacc ctttcacacc atacattttc catactgttc attaaatgat
     7861 acaaaatatg cagtccggta tgagacattc cgacttcata tatatccgga aacgcaaatg
     7921 caaatcttat atcaatatct ttatttttaa ccaccgagtt aagctcgctt ccaacgtaac
     7981 gagcaggttt ttcaacacta agaagcactc tgtcgagttt ttttacatct atcatattaa
     8041 tcccttcgac ttccttaaat tctatgatat tttaacataa taaacatttg ttgacaactt
     8101 ttattattat actcccttaa ttaaaaaata ataaaaatat taagcgaaca tttgcaggac
     8161 acgagtataa ttcttagcga atgaaggtta aaaatccgtt aaacagcttg cactgtttga
     8221 gcgaagcgag tttgcaagct gtaggatttt tagccggaat gagctagtag aattattcgt
     8281 ggccgaaacc gtgagcgtat atttttatta tttttataga aaagcaatac tgtattaaac
     8341 aattacattg aaaaagaaaa aaaagtattg taaaattaat aaaattatat tagagaggtt
     8401 attatgaatt acgacgaagc attgcaattt attcattcta catataaatt cggctcaaaa
     8461 ctcggtcttg aaagaatcaa aagacttatg gaattattgg gtaacccgca ggactgctgc
     8521 aaaatcatac atgttgcagg aacaaacggt aaaggatcaa cctgcaactt aattcatgat
     8581 gtgctgatgg aatcaggata caagacaggt ttattcatta gtccctactt agaagagttc
     8641 acggaaagaa ttcaaattaa taagcagaaa attgataaag attccctgac aagaattgcg
     8701 gaattggtaa gagataaagt taatacaatg gtttcggaag gatatgatca tcctaccgaa
     8761 tttgaggttg taaccgccat aggctttaaa tactttcagg agcagaatat tgattttttg
     8821 gttcttgaag tcggaatggg aggaagattt gatgcaacca atgtaataaa aaaacccttg
     8881 ctcagcgtta ttacttccat aagctatgac cacatggagc agctgggaaa cacattggaa
     8941 aagatagcct ttgaaaaagc aggcataata aaggaaaatt ccactgtcat aatctatcct
     9001 cagacaataa atataacaaa tgttataaag gatgtggcaa aagaaaaaaa tgcaaaagtc
     9061 tatgaagctg ataaaagcaa tatcgaaaaa acaaaatccg atattactgg acaatggttt
     9121 aaatatttaa aaagagatgt atttgagcta ccggagctaa aaataaactt attgggagaa
     9181 catcagcttt taaacgcact gactgcactt tcggctttgg aaattattaa agataaaggt
     9241 tacgacataa gcacaaaaag tatagaaaat ggtttttcaa actgtaaaca tcccggcaga
     9301 tttgaaataa taaataaaaa cccctatgtt attcttgacg gggggcacaa tattgacggc
     9361 atccgcagct ttacaaacac tttaaaggaa tatttcaagg acaaagtaac atttttcttc
     9421 ggtattttaa aagataaatc tcccgaagaa gctttgcaat taatattgcc attagcaaaa
     9481 gaaatttata ctttaactcc tttaagcccg cgagcattaa aagcagcaga cttagccgga
     9541 ctaataaaaa agtattcgga tataaaagtg attcctttgg aaaactacga agatatgctg
     9601 cctattatta aaaacgcagg caaaaatgaa ataatagcct tttcaggctc attatatatg
     9661 ataggaaacg taaggacact gttaagaaat aatggtataa caaataacct gtaaaagtcg
     9721 cataaactgg aaattcgcca agaaaaacct aaccctgttt ttcagacaag attctgaaaa
     9781 acagggttag gtcttctaaa agtctatcag ccccaagctg atttttaaac aatcatccac
     9841 ctctcccatg aggtttttat caaaacgtcc aattttttct ttaagccttc ttttatctat
     9901 tgttctgatt tgttcgagca aaacaacaga gtccttagga aggctgaaat cctcagaact
     9961 tatttcaata tgagtaggca gcttcgcctt gtttatctga gacgtaatag cagatacaat
    10021 cactgtaggg ctgtatctat tcccaatatc attttgcaca actaataccg gtcttacccc
    10081 tccctgttcc gagccaatta cggggctcaa atcggcatag tatacatctc cccttttaac
    10141 aatcaaacta ttcacttcct aataatttag tttcatagct taaaaagtct tcataatccc
    10201 cttctgagta ttcttcagta atgctcttgt tgattttact catttccaaa tagcctgctt
    10261 tcatcttgtt tctgatttca agctttcttt tttcaatcaa ataaaacctt atggatttcc
    10321 tgataaagtc acttctgttt gttccttctt cttttaccgc attatccaat tccctcaaca
    10381 aatttgcagg gatggaaatc agtacttttt tattctccgc caagatacac acctcgcaat
    10441 tgcacagtta tattatattt taacataata aacatataag ttgtatggta acatttgtat
    10501 tattcgtaaa taattattaa tattattcct caatatttat tttttcatac tgtatagctt
    10561 ttaccaattt atcatattta taaataaaaa ttgtctttgg aagcatattg tctttgctaa
    10621 aatttaatac atgcttgtat tcaccatccc aataaaccag ttcttccgga atgtcttcaa
    10681 atacatcaaa tttcccgaaa tcaaaatcat gcagcaatgt atcatttaat ttataatttt
    10741 cgattgtaac gctgctttca ggaaatttat cattgtttag tatacatctg ttccctgaaa
    10801 catatgcaca gttccatgct tcattttcag tttccgtcct gatgctgtaa ttgccgtccc
    10861 tgcaaataat attgatttta taatcatttt ccgttttatc cgtatatacc ttcatcaatg
    10921 cattagctga ataattaact ggcagaataa acggtttatc ctccgcagta caaccgctta
    10981 gagccatcag taaaactaag ataaatatta ttaatctcat accaaaacct gcttccatca
    11041 taatcgaatg ggactttgag atatatcctt taagtatggg gatatgcctt catggacttt
    11101 cttcacagtc tatccttcta tgaaggtgtg tccccatacc tacttcattt tatgatgaaa
    11161 ttttgaaata tatgccataa atcattcggc tacgcaatgt gctacggcat actcttgggt
    11221 gtgagatatt gaaatatata tgttttttat atttaactct tctgctcttt ttaatgcatt
    11281 gcttaataaa aaaacatatg gtttatcatt ttcatccttt aatatttcaa tgtgacaagg
    11341 accgaaattt gaaaatcccg ttcccaaaca tttggcaaca gcttcttttg cagcaaacat
    11401 tccggcagct gtttgaggat tgaatttcct tgtttttaaa tattctaatt ctctgtttga
    11461 atatatcttt tcaaggaacc ggatatttcc catattcttt tttattcttt caacttccgt
    11521 aatgtctatt ccagtgcctt taatcatact tcctccacag ggacattcct ttacctgatt
    11581 atgaaggtgt gtccctcatc ctttaaaaat ttttaattga caaagggctt tttgtatata
    11641 ataattatat tacatgttta gttacaatac aattcttaaa ttacatttaa caataaggag
    11701 tatcatatga ataatattac cgtagtatcc catccgctga ttcagcacaa gctgacaatg
    11761 ctgagagaca aaaacacacc cccgaaagat tttagagaat tagtaagaga aatttccatg
    11821 ctcttggcat acgaagtaac tcgaagcctg ccactgaaag atattgacat agaaacaccc
    11881 atatgcaaga ctacaggtaa aattctatct ggagcagaca ttgcaattgt acctgttctg
    11941 agagcaggcc tcggcatggt ggacggtatg ttggatttaa tcccaaatgc taaaatcggc
    12001 cacatcggtt tgtacagaaa tgaagaaact cttgagcccg ttgaatatta ttgcaagctt
    12061 ccatcagata ttgataaaag aatagtaatt attacagagc ccatgctgtc aaccggaggt
    12121 tcaatgaacg gtgcattgaa tatgctaaag aaaagaggag caaagaaaat caaatcaatt
    12181 catttggtat gcgcacccga aggactgaaa agagttacgg aaactcaccc ggatgttgaa
    12241 atctacactg ctgccataga tgaaaaatat aatgaaatag gatatatcgt acccggtttg
    12301 ggagatgcag gagacagact attcggcaca aagtgagcag acaacccaaa tttttaattt
    12361 ggtgttggtc gctcatcctg agtgaaacga aggatctgca gacaacccaa atttttaatt
    12421 tggtgttggt cgctcatcct gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nncgaaggat ctgcagacaa cacccttttg tcatcctgag tgaaacgaag gatctgcaga
    12601 caacaccctt ttgtcatcct gagcgtaagc gaaggatctg cagacaacac ccttttgtca
    12661 tcctgagcgt aagcgaagga tctcatgaga ttcttcgggc tgaagccctc agaatgacaa
    12721 aaatgttatt catagcaata acaaacagat ggagaggaca tgaaacacat atttataata
    12781 aatcctattg caggcaacgg caaattacaa gaaaagttag aaaaaactat acaccttgaa
    12841 ttaaaagata aagaaattga ttatgaggta tatatcacca aatctaaaga agacacaaga
    12901 aactttgcag aaaataaatg caaagaaaat atacaagctg tcttgtattc atgcggaggt
    12961 gacgggaccc ttcatgaaat aataaatggc gcatgcaggt ataaacatgt tagtattgcc
    13021 ataataccga taggctccgg caatgacttt attaagaatt tcaagaattc ggctaatttc
    13081 agcgatatta acctgcagac ggaaggtgaa agcatagaac tggatttaat taaggttaac
    13141 aatgaatatt ctgccaatgt attaaatatt ggtcttgatg ccgatgcagc ttacaacatg
    13201 gataaattca ggaagtttcc tttcattcaa ggcactacca gatactatct ttccatgctg
    13261 tattctttgt ttggtaaatt gggaaagcat attgaagtta atacggaaaa tggcgttatc
    13321 aacggaacat ttctgattgg tgtcatagca aacggacaat actacggcgg aggatacaag
    13381 tgtgctccaa aagcaaaagt aaatgacggt cttcttgacc tatgctttgt ggaaaaaatc
    13441 tcaagagcta aaatattaaa tataatagga agttacaaaa aaggtgagca tttagataat
    13501 cctaaaatca ataaatatat tatctatgac aagattaatc aagctaatat aaaatttgat
    13561 aaacaaacca acgtctgcat ggatggagaa atatatactt acaatgaatt aaatatatcg
    13621 acggaaaaaa acgccttgaa attttggatt ccaaaaggcg ttcaattgca aacacaacaa
    13681 accgaaaatc attcatttgt gtaaatttgg ggatagttca attttttctc atcc
//
LOCUS       DAOSHP010000047        13585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3763_length_13585_cov_7.812639, whole genome shotgun sequence.
ACCESSION   DAOSHP010000047 DAOSHP010000000
VERSION     DAOSHP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3763_length_13585_cov_7.812639"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLB33_05920"
     CDS             complement(<1..698)
                     /locus_tag="PLB33_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HOG63094.1"
                     /translation="METLMNINSAINSVVWGPVLLILVLGTGTYLSFMTGFFSITKIG
                     YVLKNTLFKMFAKDDKGEGEVTAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WLAAIMGMTTKYAEVVLAVNYREKTEDGRFVGGPMYYITNGLGKSWKWLAVLFAFFGT
                     FASFGIGCMVQSNSVALSAQSTFGIPVLATGIVLAILTAVVIVGGIKRIGAFTEKLVP
                     FMAALYILGGLWLL"
     gene            complement(884..1573)
                     /locus_tag="PLB33_05925"
     CDS             complement(884..1573)
                     /locus_tag="PLB33_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG63095.1"
                     /translation="MVLSVENLSKSFGKNMVFHDISFSVNKGEIVCIRGKSGEGKTTL
                     LRCLTSLETPDKGSIRINDMYLCKEENNIIKYAQKNELQAIRQEIGLVFQNFNLFPHM
                     NVMENLMEAPLFLKKDTHENIKNRAEELLKSLELEGKENSYPFQLSGGQQQRVAIARA
                     CMLNPSVLCFDEPTSALDDQTRGQISKIIRYLASQGIAIIIVTHDNAFADEIAQKIIT
                     MKDGRITIEEQ"
     gene            complement(1583..2239)
                     /locus_tag="PLB33_05930"
     CDS             complement(1583..2239)
                     /locus_tag="PLB33_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOG63096.1"
                     /translation="MEAKVLRMLVPMALEGLKVTMGVFAVTLIMSLPLAIVVAKLRMS
                     KNKIVSKITGTYIYIMRGTPLLLQLMFIFFGFYRVPYIGYPFSREQAIVIAFVLNYTA
                     YFAEIFRGGINSIDVGQQEASLVLGLSKTHTFMKIILPQVIKNVMPSIGNEVITLVKD
                     TSLVYILGSSDILKAARSVSNVYAAFSPYIYIGILYMVIIAILTKILDKIENKYDYYR
                     "
     gene            complement(2251..3033)
                     /locus_tag="PLB33_05935"
     CDS             complement(2251..3033)
                     /locus_tag="PLB33_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOG63097.1"
                     /translation="MKKNVLKKVKLAVLLILTAILAVGCAAQSSQPETEELIMGLDDT
                     FAPMGFRDEKGELVGFDVDLANEVAERIGVTIKFQPIDWSMKETELNAGNIDLIWNGY
                     TITAERQEKVAFTKPYLENSQIIVTLADSDINTKVDLAGKNVAVQAESSALDAINSEP
                     EVAESFGELVEFSTNNEAFSDLESGRTDALVVDEVNARYFMKQVGEEKYKVLDEDFGD
                     EEYGIGLRKEDTELLKKINDAMDEMKKDGTYDAIYAKWFSEN"
     misc_binding    complement(3262..3497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3557..3829)
                     /locus_tag="PLB33_05940"
     CDS             complement(3557..3829)
                     /locus_tag="PLB33_05940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63098.1"
                     /translation="MEFKYEKDRIYLVNQVGESIAEVTFPQISENEVNINHTYVNPAL
                     RGQGIADKLVYELASYLRKNNIKAISTCSYAIDWFEKHPEFKDVCK"
     gene            complement(4090..4299)
                     /locus_tag="PLB33_05945"
     CDS             complement(4090..4299)
                     /locus_tag="PLB33_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOG63099.1"
                     /translation="MAKEITKDMYIGEILQIDPSLAEILMEAGMHCLGCPSSQRESIE
                     EAAMVHGFDADELVKKLNSHINSAK"
     gene            complement(4354..6021)
                     /gene="hcp"
                     /locus_tag="PLB33_05950"
                     /gene_synonym="priS"
     CDS             complement(4354..6021)
                     /gene="hcp"
                     /locus_tag="PLB33_05950"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOG63100.1"
                     /translation="MGAKENLMFCYQCQEAAGCKGCTKVGVCGKKPDTAGLQDLLIYV
                     SKGLSEVTTKLRNEGKEVSADVDHLITLNLFTTITNANFDNDVFNDRIKMTLNVKSNL
                     LNQLSDKENLSEAALWTADIEEFEKKANSSEVGVLATANEDVRSLRELITYGLKGLAA
                     YMKHANVLGYDDNEINAFMQSTLAKLLDDSLTVDDLVALTLETGKFGVSGMALLDKAN
                     TSTYGNPEITKVNIGVGKNPGILISGHDLGDLEQLLKQTEGTGVDVYTHSEMLPAHYY
                     PHLKKYKHLVGNYGNAWWKQKEEFELFNGPVLMTTNCIVPPNDSYKDRLFTTGSAGYP
                     GCKHIEGNTGSEKDFTEIIELAKKCAPPTEIETGEIVGGFAHEQVFALADKVVDAVKS
                     GAIKKFFVMAGCDGRAKSRNYYTEFAKALPEDTVILTAGCAKYKYNKLNLGDIGGIPR
                     VLDAGQCNDSYSLALIALKLKEVFELQDINELPLAFNIAWYEQKAVIVLLALLYLGVK
                     NIHLGPTLPAFLSPNVANVLVEKFGIAGIGTVEDDLKIFLGDSTVTA"
     gene            6332..7045
                     /locus_tag="PLB33_05955"
     CDS             6332..7045
                     /locus_tag="PLB33_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOG63101.1"
                     /translation="MEKCNHCSHDCSHGYCIDNVAIFVDLPFEIKQDILDSSNHKTYK
                     KGEIIFNAGDYFDYLFIVIKGRVKLSKISAMGKEQILKILEVGDFMGELSLFKDTVMT
                     NFAEALENTEICIIRSEKIREIIMQKPEIAIKFLEKYAERIKHSEEIIEQIGLRDVEQ
                     RIANYLIAEVEKNNIKNRNNEYIINLPITKSVLSSILGTTKETLSRKLTLLQDEGLIR
                     MEGQRKIIITDIEGLRDMQ"
     gene            complement(7108..8283)
                     /locus_tag="PLB33_05960"
     CDS             complement(7108..8283)
                     /locus_tag="PLB33_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOG63102.1"
                     /translation="MFKISDTVSWVGKIDWELKKFHGDEYSTHKGSSYNSYLIRDEKT
                     VLIDTVWAPFSKEFVANLKKEIDLNKIDYIIANHSEIDHSGALAELMEEIPDTPIYCT
                     KNGAKILKGHHHKDWNFVEVKTGDTLDIGKNKLIFVEARMLHWPDSMMTYMSGENILF
                     SNDAFGQHYASELMYNDKVDNDELYDEALKYYANILTPFSKFVVSKIEEVVALNIPIE
                     MICPSHGIIWRDNPLQIVNKYMDWAKNYQENQIAIVYDTMWNGTRRMAEAIAEGIKSK
                     DKDVDIKLFNCAKRDKNDIITEVFKSKMILVGSSTINSGILTATAGILEMIKGMKFTN
                     KKAAAFGSYGWSGEAVKIITEQLKEAKFEVVNDGIRELWNPDEEALERCREFGRNLV"
     gene            8475..9554
                     /gene="mnmA"
                     /locus_tag="PLB33_05965"
     CDS             8475..9554
                     /gene="mnmA"
                     /locus_tag="PLB33_05965"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOG63103.1"
                     /translation="MKKKVMVAMSGGVDSSVAAVLLKDQGYDICGATLKLFSDEDVLS
                     SCSRYRTCCSLEDVEDARSVCHKLGIEHFVFNFKETFQEEVIQKFKRKYEEGSTPNPC
                     IDCNKYIKFSKLIQRAILMEKDYIATGHYARIEFDNASGRYLLKRAVDLSKDQSYVLY
                     VLTQHDLSRTLFPLGGMLKADVRKIAEERDLINARKPDSQDICFVRDGDYAGFLENMM
                     GVESKKGNFVDTEGNVIGEHKGIIHYTVGQRKGLGIALGKPVYVIDKNKELNTVTIGS
                     EEELYSKTLKATELNMIAVEKLTSTMKATVKTRYSQKETEALIHPDGEDSIIVEFIHK
                     QRAITPGQAVVFYDGDIVIGGGTIT"
     gene            complement(9547..11097)
                     /locus_tag="PLB33_05970"
     CDS             complement(9547..11097)
                     /locus_tag="PLB33_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG63104.1"
                     /translation="MAVKDRIKYTLRLRLFLFLVVLVITILLGALIILFVSGNITAGL
                     KENENFIKSEHSLIIKNTVGFYDNLAAEAVAFSRSISMSIESSLRDNDLTVDDLNNNP
                     ELLKEILSDELHQIIFYLQKSKSSGAFIILDATSNSNLPDSEYSKAGIYLQNMEPNIV
                     SSSSPTIYVLRGMADIAYKNSLPLHPQWRMEFNVADAPYYNLPMDKANKDTSLSNLYY
                     WSEAFTFPKTNEKIMMCSVPLIDIEGNVFGVCGFDVSYMLFKLLNMPDNSRYERIFCL
                     ISPIENNILKTDGSLFSGGYSARSLINNNDLKISSGKRSLYIYENNGNNFIGYHELLK
                     LYPENSSFADNEWALALLIPQEDISSVIVSTNLKFIYISAFLMILGIIISYILSKYYI
                     LPISKGFDIIKNNPKEKVKTNIFEIDELIEFLSSKTEIEKGTDSSSIILKEFLKNIKT
                     LTPAEYSVFSLYARQYNAKEIAESLCLSINTIKTHTKRIYSKLNINSKEELILYVEML
                     RESGMNIK"
     gene            complement(11103..12473)
                     /locus_tag="PLB33_05975"
     CDS             complement(11103..12473)
                     /locus_tag="PLB33_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOG63105.1"
                     /translation="MKKLVVCILIFIMLLTGCKSNEPLSAKNPVTINLWHNYGGLLKD
                     TMDLLIDEFNDTVGREKGIIINVTSLTGSATIAEKLKMAVNDEPGAPELPDITTLYPN
                     TAYMLAEKGLLVDIESLFSKEELSLFIPEFIEEGRLPDGKIYVLPTGKSTEVMFLNTT
                     IFNKFMKETNVSYNDLYTFEGLIDIAEKYYKWTDNQTPDIDNDGKAFICYDSVFNMAQ
                     TIYKQSGDELIADGSLNLSSDAFKDVWKYCFEPAVKGYSAIYDGYGSDLIKTGRVVAS
                     IGSTAGVLFYSDIVTYEDNITEPAEFLILPYPVVDGGKKVAIQRGGGMCIIKSNEAKE
                     YAAGIFLKWFTAPEQNLKFVSSTGYVPVTQMAVEMTVVNGDDIDDKIMKLQKTTKEMA
                     EQYTFFYTPVIENFDELQENYDNNIKNYAIQCKNDYFNLLSNMNETDAFNTATENKYE
                     KFTGQR"
     gene            12653..13132
                     /locus_tag="PLB33_05980"
     CDS             12653..13132
                     /locus_tag="PLB33_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63106.1"
                     /translation="MKKTSYIFIIIVLLIILTGCRAKIEEKIAEKVMEKAMGGADIDI
                     DGEEVTIKTEEGDVTFGSTEWPDSGISDKIPEFKKGNIVSVMNSEAYLLVIIEDVKEN
                     DFMDYYQEVKGEFTQESYETKSEDTITYMGSNENGISIVASYSKGDSTATISASKQE"
     gene            complement(13194..13352)
                     /locus_tag="PLB33_05985"
     CDS             complement(13194..13352)
                     /locus_tag="PLB33_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOG63107.1"
                     /translation="MQRYVCNACGYVYDPAEGDPDNGIAPGTSFEDLPDDWVCPLCGV
                     GKDEFSPE"
BASE COUNT         4216 a         2558 c         2183 g         4628 t
ORIGIN      
        1 attaataacc aaagtccacc aagaatataa agtgctgcca taaagggaac taacttttcg
       61 gtgaaagcac cgattctttt aataccgcca actataacaa ctgctgttaa tatagccaaa
      121 acaatacctg ttgctaatac cggtatacca aatgtggatt gtgctgaaag agcaacggag
      181 tttgattgaa ccatacagcc aataccgaaa gaagcaaaag taccaaaaaa tgcaaataaa
      241 acagcaagcc acttccagct cttgcctaag ccgtttgtaa tgtagtacat aggaccgcct
      301 acaaaacggc cgtcttcagt tttttctctg tagtttacag ccaatacaac ttccgcatac
      361 ttagttgtca tacccattat tgcagcaagc cacatccaaa ataccgctcc gggtcctcca
      421 agagctattg cggtagcaac acctgcaata ttacctgttc cgactgtagc cgcaagagca
      481 gtggcaactg cctggaatgc agttacttca ccttcacctt tgtcatcttt tgcaaacatc
      541 ttaaacaagg tgttcttaag tacgtagcct attttagtaa ttgagaaaaa tccggtcata
      601 aatgacaaat aagttcctgt acctaatact aaaatcaata agacaggacc ccataccacg
      661 ctgtttatgg cgctgttgat gttcattaat gtttccaaag tatccctcca ttcaaattgt
      721 tttaaaaaca taaacatctt cttgttaagt atttacattt tttaacgttt tatacaattt
      781 aatatcagac tgatactcgg gcaaataata caaggattga tttatgcatt ttgcgctgat
      841 ttaccattta taaacggcaa gtgtgatagc aaaaaaaaaa gagctattgc tcttcaatag
      901 taattcttcc atccttcatt gtaattattt tttgtgctat ttcatctgca aatgcattat
      961 catgggtaac aataattatt gcaataccct gtgaagccaa atatcttatt atttttgata
     1021 tttgacctct tgtttgatca tctaaggctg aagtaggttc atcaaagcac aatacggaag
     1081 ggttcagcat gcatgctcgt gcgattgcaa cacgctgctg ctggcctcct gacaactgaa
     1141 atgggtagct gttttctttt ccttccaatt caagactttt taaaagttct tcagctctat
     1201 ttttaatatt ttcatgagta tcctttttta aaaatagcgg tgcttccatt aaattttcca
     1261 ttacattcat atgaggaaat aaattaaaat tttgaaatac aagtccaatt tcctggcgga
     1321 ttgcctgcag ctcgtttttt tgagcatatt tgataatatt gttttcttct ttgcataaat
     1381 acatatcatt aattctaatt gagcctttat ccggagtttc caacgatgta aggcatctta
     1441 agagggttgt ttttccttct ccggatttac ctcgtatgca tacaatttca cctttattta
     1501 cagaaaagga gatatcatgg aacaccatat tttttccgaa gcttttgctt aaattttcta
     1561 ccgataaaac cataataacc tcctatctgt agtaatcata cttattttct attttgtcca
     1621 atatttttgt aagaattgca attataacca tgtataagat ccctatataa atgtatgggc
     1681 tgaaggctgc ataaacattc gatacagacc ttgcagcttt taaaatatcg cttgagccta
     1741 atatataaac caatgatgta tcctttacca atgtaattac ttcgttgcct attgagggca
     1801 taacattttt tataacctga gggagaataa ttttcataaa tgtatgcgtc ttgcttaagc
     1861 ccaatacaag agatgcttcc tgctggccaa catctatgga atttattcct cctctgaata
     1921 tttctgcaaa ataagcagta taatttaaaa caaaagcaat gacaatagcc tgttctctgc
     1981 tgaagggata tccgatatat ggcacacgat aaaatccgaa gaatataaac attaactgca
     2041 atagtaacgg cgtacccctc attatataaa tataagttcc cgtaattttt gaaactattt
     2101 tgttttttga cattctaagc tttgcaacca ctatggccaa aggaagggac ataatcaatg
     2161 taaccgcaaa aacccccata gttaccttta gcccttccaa tgccatgggc acaagcattc
     2221 tcaaaacttt tgcttccata ttacattcct ttaattttca gaaaaccatt ttgcataaat
     2281 agcatcatat gttccgtctt ttttcatttc gtccattgcg tcgtttattt ttttcaataa
     2341 ctccgtatct tcttttctca agccgatacc atattcttca tcaccaaagt cttcatctaa
     2401 tactttgtat ttttcctcac cgacttgctt catgaagtat ctcgcattta cttcatctac
     2461 taccaaagca tctgtacgcc ctgattccaa atcgctgaat gcttcgttgt ttgtggaaaa
     2521 ctctactaat tccccaaagg attcagctac ctctggctcg gaatttattg catccaatgc
     2581 actggattca gcttgcacgg cgacattttt acctgccaag tcaacttttg tattaatatc
     2641 tgagtcggct aaggtcacga ttatttggct gttttccaag taaggtttag taaatgccac
     2701 tttttcttgt ctctcggcag ttatggtgta gccgttccaa attaaatcaa tatttcctgc
     2761 atttaattcg gtttccttca ttgaccagtc gataggctgg aatttaattg ttacaccgat
     2821 tctttctgca acctcatttg ctaagtccac atcaaatcct accaattctc ctttttcatc
     2881 tctgaaaccc atgggcgcaa aggtatcatc aagtcccatt attagctctt cagtttcagg
     2941 ttgtgatgat tgtgctgcac aacctacagc caagattgcg gttaagataa gcaataccgc
     3001 caatttaacc ttttttaata catttttttt catttttctc ttctcctctt attaataata
     3061 tcacaggctc catcaccaat ttgcttattt ggggacattc ctctatctgc aaagacttac
     3121 cccatagaag aggtgtgtcc ccatactatg attgtcgcgc aaattggaat tgcgctgtgt
     3181 aagacttacc aacaatttct tttcccttcg gtcaaataaa ttgggtcaag tctataaaaa
     3241 aaactctcgt cctaggattt cctagaggac gagagaaatt cacgtggttc cacctcactt
     3301 cgctgatact tcacagcatc agcctcacta agtactaaca tactcttgcc ctgtaacggt
     3361 ggcatttccg aataagacta ctgaatttca tccatttgct caaaggtgtg ttcaatatag
     3421 gtttttatgc atttccacct accatgcact ctctataaaa aacttttata tctactattc
     3481 cttgtcacgg catttatcat tttacataca ttatcacaaa tatttataaa tgtaaagtgt
     3541 tttttaaaaa atatttttat ttgcacacat ctttgaattc cggatgtttt tcaaaccagt
     3601 ctatcgcata ggagcatgtt gatattgctt ttatattatt ttttctcaga tatgaagcca
     3661 attcataaac taatttatct gcgatgcctt gacctcgtag agcggggttt acataagtat
     3721 gatttatgtt aacttcattt tcggatattt ggggaaaagt tacttcagca atactttctc
     3781 ctacttggtt tactaggtat atcctgtctt tttcatattt aaattccata gttcctggtt
     3841 ctaaacataa tattatcctt atgcttttta tcttattgca tttacaacaa gatttccgag
     3901 tacccgacaa catatttatt tattgttggg cggtttcgaa tcaggtttgt cacctgcctt
     3961 tcttttaata ttatttacaa ttgaaatttt aatgtttaat tttcctgttg gatattaata
     4021 gggatacatt ttttataaac cattcatagc atgaaacaag agagcatgtt gtccatgctc
     4081 tcttataatt tatttggcag agtttatatg agaatttaac tttttaacca gttcatccgc
     4141 atcaaaacca tgcaccatgg ctgcttcttc aattgattct ctttgtgaag aaggacatcc
     4201 gaggcagtgc attcctgctt ccattaatat ttcggctaaa cttgggtcta tttgcagtat
     4261 ttctcctata tacatatcct tagttatttc ttttgccata aaaaacctcc tttgtactct
     4321 tgatatcatt accaatctat taattaaaat tcattatgca gtaacagttg agtctcccaa
     4381 gaatatcttt aagtcgtctt ctacagtacc gattccggct ataccaaatt tttcaactaa
     4441 tacatttgcg acatttggtg aaaggaaagc aggtaaagtc ggtcctaagt gaatattctt
     4501 aactcctaaa tatagaagcg ccaagagtac tataaccgct ttttgctcat accatgcaat
     4561 gttaaatgct aagggcaatt catttatatc ctgtaattca aatacttcct ttaatttaag
     4621 cgctataaga gccaatgaat atgaatcgtt acactgccct gcatcaagaa cccttggtat
     4681 tccgcctata tcacctaaat ttaatttgtt gtatttatat tttgcgcagc cggctgttaa
     4741 aataaccgta tcttcgggca aagcttttgc aaattccgtg taatagtttc ttgatttagc
     4801 tctgccgtca cagcctgcca ttacaaagaa ttttttaata gctcctgatt taaccgcatc
     4861 tacaacttta tcagctaagg caaatacttg ttcatgggca aaacctccca ctatttcacc
     4921 tgtttctatt tctgtcggcg gagcacattt tttagctaat tctataattt cagtaaaatc
     4981 tttttcagag cctgtatttc cctctatatg tttacatccc ggataacctg ccgaacctgt
     5041 tgtgaacaat ctgtccttat agctgtcatt aggaggaact atacagtttg tagtcatcaa
     5101 aacaggtccg ttgaataact caaattcttc tttttgtttc caccaggcat ttccataatt
     5161 gcctactaag tgtttgtatt tctttaaatg aggatagtag tgagccggca gcatttctga
     5221 atgagtgtaa acatcaactc cggtaccttc ggtttgtttt aacaattgct ccaggtctcc
     5281 caagtcatga cctgaaatta agattcccgg gtttttacct acgcctatat taacctttgt
     5341 tatttcagga tttccgtatg tgcttgtgtt tgccttatca agcaatgcca ttccgctaac
     5401 tccgaacttt cctgtttcca atgtcaatgc taccaaatca tcaactgtca atgaatcatc
     5461 aagcaacttt gccaatgtgc tctgcataaa ggcatttatt tcattgtcgt catatccgag
     5521 cacatttgca tgtttcatat atgctgctaa tccttttaat ccgtatgtaa taagctctct
     5581 taagcttctt acatcttcat tggcagtggc taaaacacca acttcacttg agtttgcttt
     5641 tttttcaaac tcttctatat ctgcagtcca taaggctgct tccgataagt tttctttatc
     5701 tgataattga tttaataagt tcgatttaac gtttaaagtc atttttattc tgtcattaaa
     5761 tacatcattg tcaaagttgg catttgtaat agtcgtaaat aagtttaaag tgattaaatg
     5821 atcaacatcg gctgatactt cttttccttc atttcttaat tttgtggtta cttccgataa
     5881 tccttttgat acatagatta ataaatcctg taatccggca gtgtcaggtt ttttgccgca
     5941 cactcctacc ttagtgcagc ctttacagcc tgccgcctcc tgacattgat aacaaaacat
     6001 caaattttct tttgcaccca tataaattgt tctcctttca attttcttat atttgttttt
     6061 atgacacgat tataatatat tttaaaaatc acttctgtaa catttgttac aataattttt
     6121 aaagaacaat tttttctgct ctttttttat acccgtcttt gtctatatat aaacaattat
     6181 aaataataag tatttattgt cacaccttta tcttatcctg agcataaacc cctatgtcat
     6241 catgagccta agcggcggat ttaatcatct tttttgttga ataaaagtaa gctaactgtt
     6301 atgattaatt aaaacagtct gggagggtgg catggaaaaa tgtaatcact gcagccacga
     6361 ctgcagccat gggtattgta tagataatgt tgccatattt gtagacttgc cttttgaaat
     6421 taaacaagac atcttggatt catccaacca taaaacttat aaaaaaggcg aaattatatt
     6481 taacgccgga gattattttg attatttgtt tattgttatc aaggggaggg ttaaactctc
     6541 taaaatctct gctatgggca aggagcaaat actaaaaatt cttgaggtgg gagattttat
     6601 gggagagttg tcgctgttca aggatacggt gatgactaat tttgccgagg cattggaaaa
     6661 tactgaaatt tgtataataa gaagcgaaaa aattcgtgaa attattatgc aaaaaccgga
     6721 aatagcaatt aagtttcttg aaaagtatgc tgaaagaatt aaacattcgg aagagataat
     6781 agagcaaata ggtttaaggg atgtggagca aagaatagca aattacctca ttgcagaggt
     6841 ggaaaaaaac aatattaaaa atagaaataa tgaatatata ataaacctgc cgataaccaa
     6901 aagtgtttta tcttccatat tgggaacaac caaagaaact ctcagccgta aacttaccct
     6961 tttacaggat gaagggctta taaggatgga agggcagagg aaaataataa ttacggatat
     7021 tgaaggatta agagatatgc aataaaagaa aatgaagaat ctcatttttg agattcttca
     7081 ctgctttcag aattaccaag aattatatta tactaagttt cttccgaatt ccctgcatct
     7141 ttctaaggct tcttcatcag gattccaaag ctccctgata ccgtcgttga caacttcaaa
     7201 ctttgcttct ttcagctgct ccgtaataat ttttactgcc tcgccgctcc aaccgtagct
     7261 gccgaatgca gcagcttttt tatttgtaaa tttcatacct tttatcattt ccaaaattcc
     7321 cgctgttgcg gtaagaattc cgctgtttat tgtagatgaa cctacaagaa tcatttttga
     7381 cttgaaaact tcagtaatta tatcgttctt atctcgcttt gcacagttaa acagtttaat
     7441 atcaacatct ttgtccttag atttaatacc ttccgcaatt gcttcagcca ttcttcttgt
     7501 accgttccac attgtgtcat aaacaattgc aatttgattt tcctgatagt ttttagccca
     7561 atccatatat ttgttaacta tttgcagagg attatctctc catattattc catggctggg
     7621 acaaatcatt tcaataggta tgtttaaagc aacaacttct tctattttgc ttacaacaaa
     7681 tttgctgaag ggagttaaga tgtttgcata atactttaat gcttcatcat acaactcatc
     7741 attatctacc ttgtcattgt acattaactc tgaagcatag tgctggccaa aggcatcatt
     7801 actgaaaaga atattctctc ccgacatgta tgtcatcatt gaatccggcc aatgaagcat
     7861 tcttgcttca acaaagataa gtttattttt accgatatcc aaggtatctc ctgtttttac
     7921 ttcaacaaaa ttccaatcct tatggtgatg acctttaaga attttagctc catttttggt
     7981 gcaataaata ggtgtatcgg gaatttcctc cattaattcg gctaatgcac ctgagtggtc
     8041 tatttccgaa tggttggcta ttatgtagtc aattttgttt aaatcaattt ccttttttaa
     8101 attagcaaca aattcttttg aaaatggtgc ccagactgtg tcaattaaga ctgttttttc
     8161 atcccttatt aaataagaat tataagatga acctttgtgg gtggaatatt catcgccgtg
     8221 aaattttttt aattcccagt cgattttacc aacccagctt accgtatcac ttattttaaa
     8281 catacttcct cctttgattt aataataaat gcactatatt acttaatata ccatatttct
     8341 ttgatttaaa tcaagtttat gttaaatatt tggttatgtc taaattattg acaatgatat
     8401 tttacaccta tataatgaat tactgtaatt ataataaact aacgaagggg tataatactt
     8461 taaaaggagt ttttatgaag aaaaaagtta tggttgcaat gagcggggga gttgattcat
     8521 ctgttgctgc cgtcttgctt aaagaccaag gttatgatat atgcggagca actttgaaat
     8581 tgttcagcga tgaagacgta ttatcgtctt gcagcaggta tagaacttgc tgctctttag
     8641 aggatgtgga agatgcgaga agcgtatgtc acaaactggg aatagagcat ttcgttttta
     8701 attttaagga aacattccaa gaagaagtta tacagaaatt caaaagaaaa tatgaagagg
     8761 gcagtacacc aaatccatgc attgactgca acaaatatat aaaattcagc aaattgattc
     8821 aaagagcaat tttaatggaa aaagattata ttgccacagg ccattatgcc agaatagaat
     8881 tcgacaatgc aagcggcaga tacttattaa aaagggctgt ggacttgtca aaggaccaaa
     8941 gctatgtact ttatgtgctg acccagcatg atttgtcaag aacacttttc ccattgggtg
     9001 gaatgttaaa agctgatgtc agaaaaatag ctgaagaaag agatttaata aatgcaagaa
     9061 agcctgacag ccaggacatt tgctttgtga gagacggtga ctatgcaggg tttttggaaa
     9121 atatgatggg cgtagaatca aaaaaaggta atttcgtaga tactgaagga aatgtaatcg
     9181 gtgagcacaa aggaatcatc cattataccg tgggacagcg caaaggactt ggaattgcat
     9241 tgggaaagcc ggtctacgta atcgataaaa ataaagaact taatacggtt accattggaa
     9301 gcgaagaaga gctttattca aaaacattga aagctaccga gctaaatatg atagctgttg
     9361 aaaaacttac ctctaccatg aaagcaaccg ttaaaacaag atacagtcag aaagaaactg
     9421 aagcattaat ccatcctgac ggtgaagaca gcattatcgt cgaatttatt cacaagcagc
     9481 gtgcaataac acccggtcag gcagttgttt tttatgacgg agatatagta ataggcggag
     9541 ggacaatcac ttgatattca ttccggattc cctaagcatt tcaacataaa gaattaattc
     9601 ttcttttgaa ttaatattta gctttgaata tattcgtttt gtatgagttt taattgtatt
     9661 gatgcttaaa cataaactct ctgcaatttc cttagcgttg tattgtctgg catacaaact
     9721 gaaaacagag tattcagcag gggtgagagt ctttatattt ttcagaaatt ctttgaggat
     9781 tatggagctg gagtctgtcc ctttttcaat ttctgttttg cttgaaagaa attctattaa
     9841 ttcgtcaatc tcaaaaatat ttgtttttac tttttctttt gggttgtttt taattatgtc
     9901 aaaccctttt gatattggta aaatataata tttgctcaaa atatacgaga taataattcc
     9961 cagtatcatt agaaaggcgc ttatatatat gaattttaaa tttgtactta ctatgacact
    10021 gcttatatct tcttgcggaa ttaacagtgc caaagcccat tcattatcgg caaaagatga
    10081 attttcaggg tagagtttca ataattcatg ataacctata aaattatttc cattgttttc
    10141 ataaatatat agagaccttt ttccggaaga aattttcaaa tcattgttgt taattaaact
    10201 ccttgccgaa tatccccctg aaaacaatga gccgtctgtt tttaaaatat tattttcaat
    10261 cggggaaatc aaacaaaaaa ttctttcata tctactgttg tcaggcatat ttaaaagctt
    10321 aaaaagcatg tagctcacat caaaaccgca tacaccaaaa acatttcctt caatatcaat
    10381 cagaggaaca gaacacatca ttattttttc atttgttttt gggaatgtaa atgcttcgct
    10441 ccaataataa agatttgata aagaggtatc cttgttcgct ttatccatgg gcaaattata
    10501 atagggtgca tctgctacat tgaattccat tctccattga gggtgaagag gcagggaatt
    10561 cttataagca atatcagcca tacccctcaa tacataaatt gtagggcttg acgaactgac
    10621 tatattcggc tccatatttt gtaaatatat tccggccttt gaatattccg aatcgggtag
    10681 attgctgttt gatgtggcat ccaaaattat aaaagcgccg ctgcttttgg atttttgcaa
    10741 ataaaagatt atctgatgaa gctcatcaga taatatttcc ttcaaaagct ccggattgtt
    10801 attaaggtca tcaacagtca ggtcattgtc cctcaaactg ctctctatac tcatggatat
    10861 agagcgtgaa aaggctacgg cttcagcggc aagattgtca taaaacccga cagtattttt
    10921 tataattagt gaatgttcac tttttataaa gttttcattt tcttttaatc ctgcggtaat
    10981 gttgcctgat acaaacagta ttatcaaagc gcctaacaat atggttatta caagaactac
    11041 taaaaacaaa aacaatctta ggcgcaaggt gtatttaatt ctatctttta cagccaaact
    11101 tctcacctct gcccggtaaa tttttcatat ttgttctcag tagccgtatt aaatgcatca
    11161 gtctcattca tattgctcaa taaattgaaa taatcatttt tacattgtat tgcatagttc
    11221 tttatattat tatcgtaatt ttcttgcagt tcatcgaaat tttctattac gggagtataa
    11281 aagaaagtat attgttcagc catttccttt gtggtttttt gaagtttcat aattttgtcg
    11341 tctatgtcat ctccattaac aacagtcatt tcaaccgcca tttgcgttac gggaacatat
    11401 cccgtagatg atacaaattt tagattttgc tcgggagcag tgaaccattt aagaaaaatt
    11461 cctgccgcat attctttcgc ttcattggat tttataatgc acataccacc gcctcgttga
    11521 attgcaacct ttttcccgcc gtctacaacc ggatagggca aaatcaaaaa ctctgccggc
    11581 tcagttatat tgtcttcata tgtaactata tcagagtaaa aaagcacacc ggcagtagat
    11641 ccaatgcttg caacaactct gccggtctta ataaggtctg agccatatcc gtcatatatg
    11701 gccgaataac ctttcacggc aggctcaaaa cagtatttcc aaacatcttt aaatgcatcg
    11761 gaagataaat tcaagcttcc gtctgcaatc aactcatcgc cggattgctt gtatattgtt
    11821 tgagccatgt taaaaacaga gtcataacat atgaaggctt tgccgtcatt atcaatatca
    11881 ggtgtttggt tatctgtcca tttataatat ttttcagcta tatctataag tccttcgaat
    11941 gtatacaagt cattatagct cacatttgtt tctttcataa atttattgaa tattgttgtg
    12001 tttaaaaaca taacttccgt agatttaccc gtaggcagca cataaatttt cccgtcggga
    12061 agccttcctt cttctataaa ttcaggaata aataatgaca attcttcttt ggaaaaaagc
    12121 gattcaatat caaccaacaa tcctttttcg gccaacatat atgcagtatt gggatataag
    12181 gttgtaatat caggcagttc aggtgcaccg ggttcgtcgt ttactgccat ttttaatttt
    12241 tccgctattg ttgccgagcc tgtgagtgaa gttacattta taataatgcc cttttctctt
    12301 cctacagtat cattgaattc atctattaga aggtccattg tatccttcaa tagacctccg
    12361 taattatgcc ataaattaat tgttacggga ttttttgcgc ttagtggttc attgcttttg
    12421 catcctgtta aaagcattat aaaaatcaat atacatacaa ccaatttttt cataatgacg
    12481 ctccatgtaa acgatttttt ttattatacc acatttaaat tatacttcca agtaaaaatc
    12541 catttaatta acccttattt catactgatt tttttaaaaa tatcaccctc agggtgatta
    12601 caattatcat aaaaaactat ataataataa caaaatcaaa gggggaagga atatgaaaaa
    12661 gacatcctat atttttataa tcattgtttt attgataatt ctcaccggtt gcagggcaaa
    12721 aatagaagag aaaattgcag aaaaggtcat ggaaaaggct atgggaggtg cagatataga
    12781 catagacggc gaagaggtaa caataaagac tgaagaaggc gatgttacat ttggctccac
    12841 agaatggcct gattccggaa tttccgataa gattcctgag tttaaaaagg ggaatatagt
    12901 aagtgtgatg aatagtgaag catatttgct ggtaataatc gaagatgtta aagaaaatga
    12961 ctttatggat tattaccaag aagttaaggg ggaattcact caagaatctt atgagacaaa
    13021 aagtgaagat acaataactt atatgggaag caatgaaaat ggaataagta ttgtggcatc
    13081 ctacagtaaa ggtgatagca cagcaacgat ttccgcgtct aaacaagaat aaaaagtgct
    13141 cttgtatctt cttaagcaaa aaaatggcga ttgctcgcca ttttttttag cctttattca
    13201 ggtgaaaatt cgtccttacc aactccgcac aatgggcata cccagtcatc cggaagatct
    13261 tcaaatgaag taccgggagc tatgccgtta tccggatctc cttcagccgg atcataaaca
    13321 taaccgcatg cattgcaaac atatctttgc atagtatcct cctccaattt tatgtaacta
    13381 acttatataa cattttaaac tatattatca aaatgtcaat taaaagattt ctttatttga
    13441 taagtagtcc ctaccttttt atagacatat ataataatta tatatataat agtgtaaaat
    13501 aatcgagagg gtattatggc tattaataaa gttgtaagag ctgcacttaa agctctgtcc
    13561 tacaatgaat atgatataaa agaaa
//
LOCUS       DAOSHP010000048        13556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3777_length_13556_cov_9.261536, whole genome shotgun sequence.
ACCESSION   DAOSHP010000048 DAOSHP010000000
VERSION     DAOSHP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13556
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3777_length_13556_cov_9.261536"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2939)
                     /locus_tag="PLB33_05990"
     CDS             complement(<1..2939)
                     /locus_tag="PLB33_05990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOG63108.1"
                     /translation="MSQITFIDAEVVPETGKIIDFGAIRPNGEYIHSASLDIIRGFLF
                     ENDFLCGHNIIAHDLKYLNKYLGQIKCVGVIDTLHLSALLFPQRPYHSLLKDDKLLTD
                     ELNNPVNDSKKAMDLFFDEIGAFKLLPEEMKKILYLLLNHKKEFKDFFQYIDYKSEGT
                     NLEAETEIRNFFNGRLCDNADFKHLIANHPIELAYCLSLINADDEYSITPPWVLMTYP
                     YVDSIMNQLRGTKCLFGCEYCNEMMDAVKGLKRFFGYNAYRSFDGVPLQEQAVKSAVN
                     GDSLLAVFPTGGGKSITFQVPALMAGRNTKSLSVVISPLQSLMKDQVDNLEKINITDA
                     VTINGLLDPLERADAIKRVENGDVHILYIAPESLRSKTIEKLLLGRKIARFIIDEAHC
                     FSAWGHDFRVDYMYIADFIKQLCEKKNLDYIIPVSCFTATAKRNVIEDIKKYFREKLN
                     LELKLYTANSGRENLTYKVIPNDRSEKYETLRNLIEYNNSPTIIYVSTTKKAEELAER
                     LTKDGYKAMAYHGRMDKKIKSQNQNEFIRGNVDIMVATSAFGMGVDKKDVGMVVHFDI
                     SDSLENYVQEAGRAGRDQNIKANCYVLFNNEDLDRHFMQLNRTKISIQEIQQIWMAIK
                     NITKMRSKISSSALEIAKKAGWDEGINDLETRVRTAILALENAGYIKRGQNTPRVYAN
                     SILVRNMKDASEKIQASKLFNDNEKQQAIRIMSKLISARSRKRASSEDAESRVDYISD
                     HLGIEKEQVIHIIQLLRQAGILSDNKDLTVFMEESSLGNKSMEKLYEFVELERFLLSH
                     ISNTTVNIKDLNELALDAGVKSSNPDKIKTVFNFWSIKGLVKRYAKGTYYLKIILLKD
                     KDEALKELEERWSIAEFILIYLQKINVNNENALEFSVLEIMGEYNFSMQLFNKKATQS
                     QIEDSLFFLSKIGILKIEGGFLVIYNAISIERLIKDNRIRYKAEDYKTLKDFYKHKME
                     MIHIVGE"
     gene            complement(3118..4464)
                     /gene="glmM"
                     /locus_tag="PLB33_05995"
     CDS             complement(3118..4464)
                     /gene="glmM"
                     /locus_tag="PLB33_05995"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOG63109.1"
                     /translation="MGKLFGTDGVRGIANKDLSPELAYKLGRVGGYFLTNGIKRPKMV
                     VGLDTRISGDMLEGALSSGLNSAGIDVLYLGILPTPAVACMIKILDADGGVMITASHN
                     PIEYNGIKFFDKDGLKLTDETENLIEEYINNNLDIDYIPISGDLGRKIRVEAPLRKYL
                     DYLKSTINVELNGLKIAVDCGNGAVYKAAPQLLHELGAQVYVIHNEPNGININVDCGS
                     TNPEEIQKLVLETGADVGLSFDGDADRLIAADENGNIVDGDHVLAICGTCLKEKGKLR
                     NNTIVGTVMTNLGLDICLKDKGINIIKTSVGDRYVLEEMLKSGYSLGGEQSGHIIFLD
                     FNTTGDGLLTAIQLLSVVKQKNAKLSELSAMMKSMPQVLVNAHVSASKKKDYLTDDVI
                     VNKIKEIEEHFSGKGRVLIRPSGTEPLVRVMIEGENQEEINFHAAELAKLIEERLQ"
     gene            4686..6602
                     /locus_tag="PLB33_06000"
     CDS             4686..6602
                     /locus_tag="PLB33_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOG63110.1"
                     /translation="MIVLSCNNVSKAYMVENIIENISFTVNDNEKVGLIGLNGAGKTT
                     LFNILTENLEPDSGEIYVAKGKKLGYLKQNTIIESNMSIMDEMLLIFDDVIRMEEKLH
                     ELSHKISSISGEKGSAELEALMNTYSELSEQFVEADGYSFRSIIKGVLKGLGFSEDEF
                     DKPINILSGGQKSRVMLAQLLLKKADILLLDEPTNHLDIEAISWLEKYIKNFKGAVII
                     ISHDRYFLDNTVNRIFLMENKYLKSFNGNYTEFVKKRKIELEQAMKQYDEYEREIERQ
                     EEMIRRLHAYGSKRNIRQAFSRQKALDKIKKMDKPSVGSQKFKLKFTPRINSGRDVLS
                     VNNVAVSFDDQQVFKNVNLKIYKGEKVGIIGPNGIGKSTLLKIIAKIIKDYSGEIEYG
                     HYVNIGYYDQEQTNLNNENTVIDEIWDEVPNLNYYTIRKYLAQFLFYGDDIYKLIEDL
                     SGGEKGRLSLLKLMTSNANFLLLDEPTNHLDIDSKEVLEDSLINYEGTILVVSHDRYF
                     LNKVVNKIYDLSEDGAFEYLGNYDYYLEKKAELDETDDIDTITKTKTQINAEKKKERE
                     IIRDRQQREKTIKELEEQIDMHENEIHELEIKLCEPSTYNDNFLIINLNEKLNIRKKE
                     LQELYLLWEELMDN"
     gene            complement(6712..7743)
                     /gene="tsaD"
                     /locus_tag="PLB33_06005"
     CDS             complement(6712..7743)
                     /gene="tsaD"
                     /locus_tag="PLB33_06005"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOG63111.1"
                     /translation="MKENINEKTLILSIETSCDETAASVVENGRNVLSNIISSQIETH
                     KEFGGVVPEVASRKHIENINDVVSKAMSEANAEPKDIDAIAVTYGPGLVGALLVGINF
                     AKGLSYAWEKPLIGVNHIEGHINANYIENKELQPPFLCLVVSGGHTHLIHVKDYGVYK
                     ILGMTRDDAAGEAFDKVARAIGLGYPGGPLIDMAAKKGKRDAIKFPRAIIDDETLDFS
                     FSGLKSAVLNYLHNSKQKNETVNTEDIAASFQEAVVDVLVMKTMKAAKQENISTIALA
                     GGVASNSRLRERFKEECLMHNYIFMRPSPILCTDNAAMIGCAGYYKYLNKRFADSSLN
                     AIPNLELAN"
     gene            complement(8154..9455)
                     /locus_tag="PLB33_06010"
     CDS             complement(8154..9455)
                     /locus_tag="PLB33_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63112.1"
                     /translation="MNNENTSERLNNSKVSTWLADNVVSLIFVAFTIFGFLVSDGVTM
                     NFFLTELSSRFFRNAFLVLSLIIPVVAGLGLNFGIVVGAMAGQIALAIVLYFGWGGVN
                     GLLLTMLIALPIATLFGYLTGLLYNKTRGQEMIASLMVGYFANGIYQFLFLFVVGVII
                     KVPNTHPMIKPDGVGVRMTVDMGAYDKGGLKYALDKIWEIPFMWAILAVFACILLLQI
                     WKIVRNKKNPTLYRYNMTMTAINIILSLVMIAISVHAITTNSSLMIVRKVPVITGLLI
                     VALCIFNELILKTKLGQDFRSVGQSQHIAEVSGINVDKTRIIATIMSTVFAAWGQIMY
                     LQNMGTLNTYGAHMQIGLFSVAALLVGGASTQKAGVKHAIFGTLLFNSMFIMSPEIGL
                     SLFGNALLGEYFRTFMVYGVIGLALGLHVWRTNKKGKITLN"
     gene            complement(9455..10555)
                     /locus_tag="PLB33_06015"
     CDS             complement(9455..10555)
                     /locus_tag="PLB33_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63113.1"
                     /translation="MVEVKKKKATFNDIVNKIGFPTIFIGGFWILLLIAGGALGISII
                     TLLSDTIKRAGMNGILVLAMVPSIQSGTGPNFALPVGIVCGLFAIVCSIEFGFTGWAW
                     LFVAIAFAIPLATVVGYLYGRLLNAVKGSEMTIATYTGFSIVAAMCLAWLMIPFQSPN
                     MGWFIGKGLRETIQLDAIGGAHILSNFLRFSLFGENGLITINGETGIIIPTGMLLMFF
                     ICCTIVWLYFRSKSGIAISAGGINPKFAQSSGINIDRCRIKANVLSTILGAIGIIVYS
                     QGYGYAQLYTAPLMMAFAAVAAVLIGGASASRAKVSNVIIGVLIYQGLFTTALPVANQ
                     LFEGTDLSEILRMVIQNGIILYALTKVKGGDN"
     gene            complement(10555..12150)
                     /locus_tag="PLB33_06020"
     CDS             complement(10555..12150)
                     /locus_tag="PLB33_06020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOG63114.1"
                     /translation="MSDNLLSLKNVSKEYSGNRVLKDINIELKKGQIHALIGENGAGK
                     STLMNILFGMSVIHTTGGFEGTIEIEGKQVDIKSPHDAMDYGIGMVHQEFMLIPGFTI
                     MENIKLNREITKPNLVSPIFGKRLETLNIKRMNEDTRKALDVLDMDIEEYVQVAGLPV
                     GHMQFIEIAREIDKTGLKILVFDEPTAVLAETEAANLLKAMKKLAQKGIGILFISHRL
                     DEIIEVADVVTILRDGEHVVTKNTEDTNKYEIAQLMVGRSITIEREESTNRNIVDETI
                     LDIKNLHVAMPGERVKGVDLKVKKGEIVGIGGLAGQGKLGIANGIMGIFPAEGEVEFK
                     GKKSALNNPKSAIKSGIAFVSEDRKGVGLLLDESIESNIAFSAMQINDEFLVNYGPIK
                     LKDSAKIREHANKMIKDLDIRCTSSLQRVGSLSGGNQQKVCVARALTMKPELLLVSEP
                     TRGIDIGAKKLVLDLLKQLNNEGMTIIMTSSELAELRSICDRILIICEGKVEGVLPPD
                     APDAEFGLMMSGSRNGKSEGSEA"
     gene            complement(12270..13493)
                     /locus_tag="PLB33_06025"
     CDS             complement(12270..13493)
                     /locus_tag="PLB33_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOG63115.1"
                     /translation="MKRLLSLLLALMMVLSLAACTQPAQTPPAEEKPAEEKPAEEKPA
                     EEKPAEEEPFEGIIAIVTNTLSQNEEEYRSAELMVQTYGEDKIMHVLWPDNFMTEQEQ
                     MISTIAKIGSNPDVKGLIINQAVPGTNAAVDKLLETRDDIFISYCTPQENPPDVAERA
                     DLILNPDELKMGDAIPIQAQKLGAKTLVHYSFPRHMSIVMLAARRQLMIDKCKEIGLE
                     FYDATAPDPTSDVGVPGAQQFILEDVPKLVDRFGKDTAFFSTNCAMQTPLIKATSDAG
                     AIYPQPCCPSPYHGYPSALGIESTGYSVDAMANVISETAKALKAKGVLGRFSTWPVPV
                     AMMNTVASTEYIIEWINGNVGDELDVEVLEEKMAEYAKLAVTTSSYTEEGLEIPHFRL
                     IMMDFLTYGEEHILD"
BASE COUNT         4081 a         2690 c         2212 g         4573 t
ORIGIN      
        1 tattcgccca ctatatgtat catttccatc ttatgtttgt aaaaatcttt aagagtctta
       61 tagtcctcag ccttgtatct aatcctgtta tccttgatta atctctctat actgattgca
      121 ttgtatataa caagaaagcc cccttcgatt tttagaattc ctatttttga taagaagaaa
      181 agtgaatcct ctatctgcga ttgggtggct tttttattaa aaagctgcat gctaaaatta
      241 tattctccca taatttcaag gacagaaaat tccaaggcat tttcattatt tacatttatt
      301 ttttgaaggt atattaggat aaattctgca atagaccacc tttcttctaa ctccttcaag
      361 gcctcatctt tatcttttaa aagaatgatc ttaagataat aagtaccttt tgcatatcgc
      421 tttacaagac cctttatcga ccaaaaatta aagactgttt ttatcttatc aggattagaa
      481 ctttttacac cggcgtccaa agcaagctca tttaaatctt ttatatttac ggttgtgttt
      541 gaaatatgcg aaagcaaaaa acgctccaat tcaacaaatt cgtaaagttt ttccatggat
      601 ttgtttccta aactgctctc ttccataaaa acagtcaaat ctttattatc ggataatatg
      661 cccgcttgtc ttaatagttg aataatatgt ataacctgtt ccttctcaat tcccaaatga
      721 tctgaaatgt aatcaaccct ggattctgca tcttcactgc ttgccctttt tcggcttctt
      781 gctgatataa gtttgctcat tattcttata gcctgctgtt tttcattatc attaaacaac
      841 ttggaagctt gaattttttc tgatgcatcc ttcatgtttc ttaccaatat actgtttgca
      901 tatactctgg gtgtgttttg ccctcgctta atataacctg cgttttccaa agctaaaatt
      961 gccgttctga ctcttgtttc aaggtcatta ataccttcat cccagccggc tttcttggca
     1021 atttccagtg ctgaacttga tatctttgat ctcatcttgg ttatattctt tattgccatc
     1081 cagatttgct gtatttcttg tatgctgatt tttgttcgat tcagttgcat gaaatgcctg
     1141 tctaaatcct cattattaaa taaaacataa caattggctt ttatgttttg gtctctgccg
     1201 gctcgtccag cttcctgaac gtaattttcc agtgaatcgg aaatatcgaa atgtacaacc
     1261 attcccacat cttttttatc tacacccata ccaaatgctg aagttgcaac cataatgtca
     1321 acattaccac gaatgaattc attctggttt tgacttttta tttttttatc cattcgccca
     1381 tggtaagcca tggctttata tccgtctttg gtaagtcttt ctgcaagttc ttcggctttt
     1441 ttagtggttg aaacatagat gatggtagga gaattgttgt attcaataag gtttctcagt
     1501 gtttcgtatt tctcggatct atcattaggt attactttgt aggtgaggtt ttccctccct
     1561 gaattcgcag tataaagctt taattccaaa ttgagttttt ctctgaaata tttcttaata
     1621 tcttctataa catttcgttt tgctgtagct gtaaagcatg aaaccggtat tatgtagtca
     1681 aggtttttct tttcacacag ctgtttaata aaatcagcaa tatacatata atcaactcta
     1741 aaatcatgcc cccaggcaga aaaacaatgg gcttcatcaa ttatgaatct tgcaatctta
     1801 cggccaagta aaagtttttc aatggttttc gacctaagag attcaggcgc aatatataat
     1861 atatgaacat cgccgttttc aactctttta atggcatctg ctctttctaa tgggtctaaa
     1921 agcccgttga tagttacagc atcagtaata ttgattttct ctaaattatc aacctggtct
     1981 ttcataagag attgtagtgg tgaaattaca acactaagag attttgtatt ccttccagcc
     2041 ataagtgcag gaacttggaa tgtaatagat ttgcctccgc ccgtggggaa aactgctaaa
     2101 agagaatctc cgtttactgc gctttttact gcttgttcct gcaagggaac accatcgaaa
     2161 cttctgtatg cgttataacc aaaaaacctt tttaagcctt ttactgcatc catcatttca
     2221 ttgcaatact cacaaccaaa aagacacttt gtgccccgaa gttgattcat gatactatct
     2281 acatatggat atgtcataag tacccaagga ggcgttatgg aatactcatc atctgcattt
     2341 ataagagaaa ggcaataagc aagctcaatc ggatgattag ctataaggtg tttaaaatct
     2401 gcattatcgc ataatctgcc attgaaaaaa ttccttatct ctgtttcagc ttctaaattt
     2461 gtaccttcac ttttgtaatc gatatattga aagaaatctt taaactcctt tttatgattc
     2521 aataataaat ataatatctt tttcatttcc tctggcagta atttaaaagc tcctatttca
     2581 tcgaagaata aatccattgc cttcttagaa tcatttacag gattgtttag ctcgtctgtt
     2641 aacagcttat cgtcctttaa caatgaatga tagggtcttt gaggaaataa aagagcagat
     2701 aaatgcaagg tatctataac accgacacat ttaatctgcc ctaaatattt atttaaatat
     2761 tttaaatcat gtgcaataat attatgtccg cataaaaagt cgttttcgaa aagaaatcct
     2821 ctgatgatat caagagaagc gctatggatg tattctccgt ttggtcttat agcaccaaag
     2881 tcaatgattt ttcctgtttc cggcactact tccgcatcaa taaatgttat ttgactcata
     2941 atacccgtat ccttagtcga attatcttat tttagctaaa ttatatagca tattggaaaa
     3001 aatttcaatg aaaacaagaa aattcatctg gtaacataga gacggacctt atatagattg
     3061 agaaatactc tgatagcaaa aagaaccgtc caaggaaacg tctgaaacag aaacttttta
     3121 ttgaagtcgc tcttctatca gttttgctaa ttcagcggca tggaagttta tttcttcctg
     3181 gttttctcct tcaatcataa cccttacaag gggctctgtt cccgatggtc ttattagaac
     3241 ccttccctta ccgctaaaat gttcttcgat ttctttaatt ttatttacga ttacatcatc
     3301 agttaaataa tctttctttt ttgatgctga aacatgagca ttcaccaaga cctgaggcat
     3361 acttttcatc atcgcggaaa gctctgaaag ctttgcattt ttttgtttta ctacacttaa
     3421 cagctgaatg gctgtcaaaa gtccgtcgcc tgtggtgttg aagtccaaaa atattatgtg
     3481 tcctgactgc tctcctccca atgaataacc gctctttaac atttcctcca aaacatatct
     3541 gtcgcctacg ctggtcttta taatattaat acccttatcc ttaaggcata tatcaagacc
     3601 taagtttgtc ataactgttc cgactatggt gttgtttctg agctttcctt tttctttcaa
     3661 gcaagtgccg catattgcca aaacatggtc gccgtcaaca atatttccgt tttcatccgc
     3721 tgcaatcagt ctatcggcat ccccgtcgaa agatagtccc acgtcagctc ctgtttctaa
     3781 aacaagcttt tggatttctt ccgggtttgt agagccgcag tctacattaa tgtttatacc
     3841 gttaggctca ttgtgaataa cataaacttg tgctccaagc tcgtgaagaa gctgaggtgc
     3901 ggctttataa acagcgccgt ttccgcaatc gactgcaatt ttaagcccat ttagctccac
     3961 attaattgta ctctttaagt aatccaaata ttttcttaat ggggcttcaa cacgtatttt
     4021 tcttcctaag tctccggaaa tcggtatgta gtctatatcc aaattattat ttatatattc
     4081 ttcaattaag ttttctgttt catccgttaa tttaagtcca tccttgtcaa aaaactttat
     4141 tccgttgtat tcaataggat tgtgagatgc agttatcatc actcccccgt cagcatcgag
     4201 tattttaatc atgcatgcga ccgcaggtgt gggcagtatt cccaaatata aaacatctat
     4261 cccggctgaa tttaatcctg aagacagtgc accttccaac atatctccgg atattctcgt
     4321 atccaagcca acaaccatct ttggtctttt tattccatta gttaaaaaat atcctcctac
     4381 tcgccctaat ttatatgcta attcagggga caaatcttta tttgcaatac ctcttactcc
     4441 atctgtgcca aatagtttac ccattcttcc tccaaacaaa aaaatttaat caatatccgc
     4501 cgtctaaaaa gaggggttat ctttttcgtt atattgtatc atattttata ctctaaaaaa
     4561 agataccaaa atattaattt tttatgatat aatatcggtg tgaagcaagc cgattttgcg
     4621 gcttgtactt atgatataat gaaaactaat cattcataaa cttgagcact tgaaaggata
     4681 cggttatgat tgttctatca tgcaacaatg tttcaaaagc ttatatggtg gaaaatataa
     4741 ttgaaaatat ttcttttacc gttaatgata atgaaaaggt tggattaatc ggtcttaacg
     4801 gagcaggtaa aacaaccttg tttaatatat tgacagaaaa tctggagccc gattccggcg
     4861 aaatatatgt tgctaagggt aagaaacttg gctatcttaa gcaaaacaca attattgaaa
     4921 gcaatatgag cattatggat gaaatgcttt taatttttga tgatgttata aggatggaag
     4981 aaaagctcca tgagttatcg cataaaatta gcagtatttc gggagaaaaa ggctctgcgg
     5041 aacttgaagc actaatgaac acatattcag aattgagcga gcagtttgtt gaagctgacg
     5101 gttacagttt tagaagcata attaaaggag ttttaaaagg tcttggattt tcagaagatg
     5161 aatttgataa acctataaac attttaagcg gcggtcaaaa aagcagagtt atgcttgcac
     5221 agcttttgct caaaaaagca gatattcttt tgctggatga acctaccaat catttagata
     5281 ttgaagcaat atcttggctt gaaaaatata taaagaattt taaaggtgcg gtaataatta
     5341 tttctcacga cagatacttc cttgacaaca cggtaaacag aattttcctt atggaaaaca
     5401 aatacttaaa atcgtttaac ggaaattata cggaatttgt gaaaaaaaga aaaattgagc
     5461 ttgaacaagc aatgaaacaa tatgatgaat atgaaaggga aattgaaaga caggaagaaa
     5521 tgattcggcg cctccatgca tacggaagta agcgaaatat cagacaggca tttagcaggc
     5581 aaaaggcttt ggataaaatc aaaaaaatgg ataagccgtc tgttggaagc caaaaattca
     5641 aattaaaatt tacaccgaga atcaatagcg gcagggatgt gttaagtgtt aataatgtag
     5701 ctgtatcttt tgacgatcaa caagttttca aaaatgttaa tttgaaaata tataagggag
     5761 aaaaagttgg aattataggt cctaacggta taggcaaatc aactctctta aaaattattg
     5821 ccaaaataat aaaggattac agcggggaga tagaatatgg tcattatgtc aatatcggat
     5881 attacgacca ggagcaaaca aacttaaata atgagaatac tgtaatcgat gaaatatggg
     5941 atgaagtccc taacttaaat tattatacaa tccgaaaata tcttgcacaa tttttgtttt
     6001 acggagatga tatttataag ctcatcgaag atttgagcgg cggtgaaaaa ggaaggctgt
     6061 cacttttaaa acttatgact tctaatgcaa attttttgtt gttggatgaa cctacaaatc
     6121 atttggatat tgattcgaag gaagtattag aagattcact tattaactat gaaggaacca
     6181 tattggttgt ttctcatgac agatatttct taaacaaggt tgtaaataag atatacgatt
     6241 tatcggaaga cggagcattt gaataccttg gcaactatga ctactatctg gaaaaaaaag
     6301 cagagcttga tgaaacagat gatattgata ctattacaaa gactaagaca caaataaatg
     6361 cggaaaagaa aaaggaaagg gaaattatca gggacaggca acagcgggaa aaaaccatca
     6421 aagaacttga agagcaaatt gatatgcatg aaaatgaaat tcatgaatta gaaatcaaat
     6481 tatgcgaacc ttcgacatat aatgacaatt ttttaattat aaatctgaat gaaaaattaa
     6541 acattcgaaa aaaagagttg caagagcttt accttctatg ggaagaatta atggataact
     6601 aaaaaaagtt atccaccata tgtttgtagt atgtgcataa ctttttactg tattacttgt
     6661 atcaacaact ttatccacag ttgttgatac cttgttttta taggctgtta attaatttgc
     6721 aagttctaaa tttggaattg catttaagga tgaatctgca aatcttttgt ttaaatattt
     6781 ataatatccg gcgcagccaa tcatggctgc attatctgtg cataaaatcg gagaaggtct
     6841 cataaatata tagttatgca ttaaacattc ttccttaaat ctttccctta aacggctgtt
     6901 tgacgcaact ccgccggcta atgcaatagt gcttatattt tcctgttttg cagccttcat
     6961 tgttttcata accaacacgt caaccactgc ctcctgaaaa cttgctgcaa tatcttcggt
     7021 attgacagtt tcattctttt gctttgaatt atgtaaataa ttcaaaaccg ctgattttaa
     7081 cccgctgaaa ctaaaatcca gtgtttcatc atcaattatc gctctgggaa acttgattgc
     7141 atccctcttt ccttttttgg ctgccatgtc aatcaaaggt cctcccggat atcccaatcc
     7201 gattgctctt gccaccttgt caaaagcttc tccggcagca tcatctctgg tcattcctaa
     7261 aattttatat acaccgtaat ccttaacatg aatcaggtga gtatgaccgc ccgagaccac
     7321 aagacagaga aatggcggtt gaagctcttt attttcaata tagtttgcat taatatgtcc
     7381 ttcgatatga tttacgccaa tcaagggttt ttcccatgca taggaaagtc cttttgcaaa
     7441 attaataccc accaacaatg ctcccactaa accgggaccg tatgttacag caattgcatc
     7501 tatgtccttt ggctctgcat tggcttcact catggccttt gatactacat catttatatt
     7561 ttcaatatgt ttccttgaag caacctcagg tacaacacct ccaaactcct tatgagtttc
     7621 tatttgagat gatattatat tggataaaac atttctcccg ttttcaacaa cagaagcagc
     7681 agtttcatca caggatgttt ctatggaaag tattaaagtt ttttcgttta tattttcctt
     7741 catgcgcaat attttcctcc gtattttcct gaactgatta tactactgtg agtttcataa
     7801 ttcaagaaga aaaaatgtaa cagtaccccc gtccctttgt cagaattcaa gaagaaaaaa
     7861 agagatcctt cgctgcgctc aggatgacaa agcgtcatac cgaacaatca ggaagaatct
     7921 catactaacg tgtccccata cctctaataa atggtaaata ataaagatac aaagcgaaca
     7981 tttgcaggac acgactataa ttcttagcga atgcaggtta aaaatccgtt taacagcttg
     8041 cattgtttga acgaagtgag tttgcaagct gtaggatttt tagccgtaat gagctttaga
     8101 attattcgtg tccgaaaccg tgagcgtgta tttttattat ttttatattt taattagttt
     8161 agggtgattt tgcctttttt gtttgttctc caaacgtgga gtcccaatgc aagacctata
     8221 accccgtata ccataaaggt tctgaagtat tctcccaaaa gggcattgcc aaacagtgac
     8281 aatcctattt caggagacat tataaacatt gagttaaaca atagtgtacc aaaaattgca
     8341 tgtttcactc ctgccttttg agttgaagct cctccaacca aaagtgccgc tacagaaaac
     8401 aatcctattt gcatgtgggc accgtatgtg tttagggtac ccatattctg caggtacatt
     8461 atttgtcccc atgcagcaaa tacagttgac attattgttg caataattct ggttttatca
     8521 acattaatgc ctgatacctc agcaatgtgc tggctttggc cgacacttct gaaatcctgt
     8581 cccagttttg ttttcaaaat caattcattg aatatacata aagctactat caataatccc
     8641 gttattaccg gaacctttct aacaatcatt aaacttgagt ttgttgtaat tgcatgaacg
     8701 cttattgcaa tcataaccaa gctcagaata atgtttattg ctgtcatggt catgttatat
     8761 ctgtacaagg tgggattctt tttgtttctt acgattttcc atatctgcag gagaagaata
     8821 catgcaaata ctgccaatat agcccacatg aacggaattt cccaaatttt gtccaatgcg
     8881 tatttaagtc cgcccttgtc atatgccccc atgtctactg tcattctcac acctacgccg
     8941 tcaggtttaa tcatgggatg agtattggga acttttatta ttacacctac aacaaacaag
     9001 aataaaaatt gatatatacc gtttgcgaaa taacctacca tcaggctggc aatcatctcc
     9061 tgtcctcttg ttttgttata gagcaaacct gttaaatatc cgaacaaggt tgctattggg
     9121 agtgcaataa gcattgttaa caaaagtccg ttaacaccgc cccatccaaa atataatacg
     9181 attgcaagtg ctatttgtcc tgccattgca ccgacaacta taccaaagtt tagcccgaga
     9241 cctgccacta ccggtattat caaagataaa acaagaaatg cattcctgaa gaaacggctt
     9301 gataattccg tcaggaaaaa gttcattgtt acaccgtctg atactaaaaa tccaaatatt
     9361 gtgaaggcaa cgaatattaa tgaaacaaca ttatctgcaa gccatgttga tactttagaa
     9421 ttatttaatc tttcggaagt attttcatta ttcattaatt atcgcctcct ttaacctttg
     9481 tcaacgcata aagaattatt ccgttttgga taaccatacg aagtatttcg gataaatccg
     9541 ttccttcaaa cagttggttt gccacgggaa gcgcggtggt aaaaagaccc tggtatatta
     9601 atacgcctat tattacgtta gatacctttg ctcttgaagc agaagccccg cctattaata
     9661 cggcagcaac cgcggcaaat gccatcataa gcggtgccgt atagagctgt gcatagccgt
     9721 aaccctggga ataaacaata ataccgatgg ctcccaaaat tgtagataaa acatttgctt
     9781 ttattctgca tctgtcaata tttatacctg aagattgggc aaattttgga tttattccac
     9841 ctgccgatat ggcaattccg gattttgacc taaaatacag ccatactatg gtacaacata
     9901 tgaaaaacat taaaagcatt cccgttggga ttattattcc ggtttcaccg tttatggtga
     9961 taagcccatt ttctccaaat aatgagaacc ttaagaaatt acttaatata tgtgctccac
    10021 cgattgcatc taactgtatt gtttctctta aaccctttcc tataaaccat cccatattgg
    10081 ggctttgaaa cggtatcatg agccatgcaa ggcacattgc tgcaacaatc gaaaatcctg
    10141 tgtaagtggc gattgtcatt tctgaacctt taacagcatt tagaagtctt ccgtacaagt
    10201 atcctacaac tgtcgccaat ggtattgcaa aagcaattgc tacaaataac catgcccaac
    10261 ccgtaaatcc aaattctatg gagcagacta tggcaaataa gccgcataca atacctaccg
    10321 gcaatgcaaa gtttggacct gttcctgatt gtattgaagg aaccattgcc aataccagta
    10381 taccgttcat acccgcacgt tttatcgtgt cgcttaataa agtaataatg gatattccca
    10441 aagctcctcc ggcaataagc aagagtatcc aaaaaccgcc tataaaaatt gtaggaaatc
    10501 ctattttatt aacaatatca ttgaaagtgg cttttttctt ttttacttct accattatgc
    10561 ttcactccct tctgattttc catttcggct gcctgacatc atcaaaccaa attcagcatc
    10621 cggtgcatcg ggaggaagta ctccttcaac tttcccctca catattatta aaattctgtc
    10681 acatatgctt ctcaactcgg ctaattcgga ggaagtcatt attattgtca tgccttcatt
    10741 atttaattgc ttgagtaagt caagcactaa ttttttagcc cctatgtcga ttcctctcgt
    10801 aggctcggaa actaaaagca gctccggttt cattgtcaat gcccttgcaa cacaaacctt
    10861 ttgctggttc ccgccgctta ggcttcctac cctctgcaag gaggaggtgc atctaatatc
    10921 caaatctttt atcatcttat tggcatgctc tcttatttta gcagaatctt tgagtttaat
    10981 gggaccataa tttactaaaa attcatcgtt tatctgcatg gcggaaaatg caatattgga
    11041 ttctattgat tcatccaata aaaggccgac gcctttcctg tcttcgctta caaaagcaat
    11101 gccggattta attgcagatt tggggttatt cagagctgat tttttgccct tgaattcaac
    11161 ctctccttca gctggaaata ttcccattat gccgttggca atacctaatt tcccttgacc
    11221 tgcaagtccg cctattccta ctatttcacc tttttttact tttaagtcaa cgccttttac
    11281 tctttcaccg ggcattgcaa catgtaaatt ttttatatct aatattgttt catcaacaat
    11341 atttctgttc gtagattctt ctctttcaat agtgatactt cttccgacca tcaactgtgc
    11401 tatttcatat ttgtttgtat cttctgtatt ttttgtaact acatgctctc cgtctcgcag
    11461 aatggttact acatccgcta cttcaataat ttcatcaagt ctgtggctta taaataatat
    11521 gccgattccc ttctgggcaa gctttttcat tgctttcaaa agatttgcag cttctgtttc
    11581 cgctaatacc gcagtgggct catcaaaaac aagaattttt aatcctgttt tatcaatttc
    11641 tcttgcaatt tcaataaatt gcatatgacc aacaggaagg cctgctactt gtacatattc
    11701 ttctatatcc atgtccaaca catccaaggc tttccttgtg tcttcgttca ttctttttat
    11761 atttaatgtt tccaggcgtt ttccgaatat ggggcttact aagttgggtt ttgtaatttc
    11821 cctgttcaac ttaatatttt ccataatagt aaacccgggg ataagcataa attcctgatg
    11881 taccatgccg attccatagt ccatggcatc atgaggactt ttgatatcta cctgtttgcc
    11941 ttctatttct atggttcctt caaatccgcc ggttgtatga attaccgaca ttccaaatag
    12001 aatattcatc aatgtggatt ttcctgcgcc gttttcgcct ataagcgcat gaatttgacc
    12061 tttttttaat tcaatattta tatccttcaa tactctattt cctgaatatt cttttgaaac
    12121 attttttaag cttagaaggt tatcactcat tactacctcc tgtttattga cataactaat
    12181 tagcaaagga atgaatcaga agttcctaat aagaaattct gaaacattcc ttaattttta
    12241 cattatttaa ttatagtact atatatcgat taatcaagaa tatgttcttc tccgtaagtt
    12301 aagaaatcca tcatgattaa tctgaaatga ggaatttcta atccttcttc tgtatatgaa
    12361 cttgtagtaa cagctaattt tgcatattca gccatttttt cttcaagtac ttctacatca
    12421 agctcatcgc ctacattacc gttaatccat tcaatgatat attctgtaga agctaccgta
    12481 ttcatcatag caacaggtac cggccatgtt gagaatcttc ctaatactcc ttttgccttc
    12541 aatgctttgg ctgtttcgct tattacgttt gccatagcgt caacggaata acctgtagat
    12601 tcaataccta atgcagatgg atatccgtgg tatggtgacg ggcagcaagg ttgcggataa
    12661 attgctcctg catcgctcgt tgctttaata agcggagtct gcattgcaca gtttgttgag
    12721 aagaatgctg tgtcttttcc aaatctatca actaacttcg gtacatcttc aagaataaac
    12781 tgctgtgctc ccggtactcc aacgtcactt gtcgggtcag gtgctgttgc atcatagaat
    12841 tcaagtccga tttctttaca tttatcaatc attaattgac gtcttgcagc aagcattacg
    12901 attgacatat gtctcgggaa tgaatagtgt accaatgttt tcgcacccaa tttctgtgct
    12961 tgtataggaa tagcatctcc catcttcaat tcgtcagggt taagtattaa gtctgctctc
    13021 tcagcaacgt caggtggatt ttcttgtggt gtacagtaag aaataaatat gtcatcacga
    13081 gtttccaaga gtttatctac tgctgcgttt gttcccggaa cggcctgatt tataatcaat
    13141 cccttaacat ccgggttaga gcctattttt gcaattgtag aaatcatttg ttcctgctcg
    13201 gtcatgaaat tatcaggcca taatacgtgc atgattttgt cttcgccata tgtttgaacc
    13261 ataagttcag ctgaacgata ttcttcttcg ttttgagaaa gagtgttcgt aacaatggct
    13321 attatgcctt caaacggttc ttcttctgca ggcttttctt ctgcaggttt ttcttctgcc
    13381 ggtttttctt ctgccggttt ttcttctgcc ggcggagttt gtgcaggttg tgtacaagct
    13441 gctaacgata aaaccatcat cagagccaac aataaactta ataatctttt cattacttgt
    13501 ccctcctaat tttacacata aaaatatgta ttcatattat aattgaatat acttct
//
LOCUS       DAOSHP010000049        13501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3799_length_13501_cov_8.925480, whole genome shotgun sequence.
ACCESSION   DAOSHP010000049 DAOSHP010000000
VERSION     DAOSHP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13501
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_13501_cov_8.925480"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PLB33_06030"
     CDS             complement(<1..630)
                     /locus_tag="PLB33_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOG63116.1"
                     /translation="MSRIYTIKAEYHDVENLTKILSEHNEIQFVSFMGVDIGGNGTDE
                     KIPVKLFIKDMEEMLKYGIQTDGSSVELQGIADLSDARVDLLPDTDCTWFVDYNYEYC
                     NDENGLPVGTLIIPSFIVHKNKLVCSRSTLRRAEVHFKNEIKNVLKNHPHIAESLGFK
                     YEDIDEVLLTAATELEMWVKTPEQSADFEELHASQVLKEQYWKRTMGTVR"
     gene            complement(642..1271)
                     /locus_tag="PLB33_06035"
     CDS             complement(642..1271)
                     /locus_tag="PLB33_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG63117.1"
                     /translation="MKYKYVIFDFDGTLADTEEANFVIYQKLAEKYNLRNITMDELGR
                     LKKMHAKEVMAYIQLKKRYLPFLLKRGKTLLTQDIKNISLCKPDIFHIIGRLRNMGIK
                     TGIITTNSKTNVKLFLETHNVDVFDTIVSAAMFGKESKMKKIARKAKMSLDKFLYVGD
                     EIRDITAARNAGMDIASVGWGYNTVESLRKHEPDYLIFEPSELIDICKG"
     gene            complement(1369..1947)
                     /locus_tag="PLB33_06040"
     CDS             complement(1369..1947)
                     /locus_tag="PLB33_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63118.1"
                     /translation="MPPISTVMHFGKKFMESYDKVDIPNFIIGIILPSTLNDDQLEEL
                     HNVDEDGNIDVREFYEKFDFKTMSLKDSSIVLGYYCHLWLDEYYKFNASKLKIHNKQN
                     LKGEELNSAVKNLLNYYDKKAIGSFYEKYTEDIKAVQSYIFAASNIDNSKKKLLEYLN
                     ETVPDEVITGLIKEEQYMSFIREGCGKIIKSL"
     gene            complement(2113..3300)
                     /locus_tag="PLB33_06045"
     CDS             complement(2113..3300)
                     /locus_tag="PLB33_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63119.1"
                     /translation="MNNKNYRVLKFFEPVNKKDKSRGYVRLDMRGNRGNMIVAAENLG
                     DAKSSSEVYLYKDKHNKIKLGDINNKKGIIKKKLTFENSNAIEDYNVCAVVKDGKIVL
                     YSSLFSVSSQDIVRKLEEDDNEQEKDIFADKKQKFQESKPIHTKEKEQINIKDTRGAI
                     EPKEVIETKEIIEPKGLREVREIREKGMAKAAENKPEDETKEEVKKVVQEPVKTIKVK
                     ETIKEDSEETMETPEKSSKKDDISNQGYRNRFDEKLYKTLREFKEIEPLSVKLKNFNW
                     WYIPYDEMGIKTGVLPYYNQIISAYYPYPMSNRVTTCTSLMKKYGHYIFGIYKENDEI
                     ARFIYGVPGEFTKEEQPYKGVTGFKNWSYANKDNKGESGYWLAFVNPKTGEITDPPQV
                     SLT"
     gene            complement(3376..4659)
                     /locus_tag="PLB33_06050"
     CDS             complement(3376..4659)
                     /locus_tag="PLB33_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOG63120.1"
                     /translation="MIIHVVQPGETIYSIANQYNISPQKIIIDNELENPNVLVPGQTL
                     VILIPDVIHVVQPGETLADIARQYNTTTVNLLQLNPFIEYADQIVPGDQITISNTADK
                     RGLIHVLGYAYPHIDRTVLLKTLPYLTYLTIFTYGITEEGELIDIEDEDIIRMARDFG
                     VAPLMLISTLTEEGTFSNELAHVILNDPVAQNNLIENVLQNLREKGYYGLDIDFEYVL
                     PEDEEAFINFIQNVHDRISPEGFVLFTALAPKTSADQPGLLYEAHNYPRIGEASDRVL
                     LMTYEWGYTYGPPMAVSPVDRVREVLDYAVTEIPREKIYMGIPNYGYDFILPYVQGVS
                     RADSVSNVEAVDLAADVGAYIQYDEVSQAPYFIYYDEQGRQHRVWFEDARSILAKLDL
                     FSEYGFEGVGYWNVMRYFPQNWLLVSSLYDIAKLV"
     gene            complement(4796..6211)
                     /gene="gatB"
                     /locus_tag="PLB33_06055"
     CDS             complement(4796..6211)
                     /gene="gatB"
                     /locus_tag="PLB33_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOG63121.1"
                     /translation="MSYELVAGLETHIELSTKTKIFCQCTTEFGGEPNTHCCPICIGM
                     PGTLPKLNKKVVEYAVKAGIALNCKIRNISKMDRKNYVYPDLPKAYQISQFDKPICYG
                     GYIKLSSGKNININRIHIEEDAGKLVHERGNTYIDYNRGGVPLIEIVTEPDFSSIDEV
                     LEYLEKLRLVMKYIGVSDVKMQEGSLRCDVNISVRKKGEEKLGTRTEIKNMNSLSFIA
                     KAMEYEFERHVDILESGGEIEQETRRYDSELNITESMRGKEDAEDYRYFKEPDLVDII
                     VSEEEIKKLKSELPQLPDEKLHVFINEYSLPEDDAELLIKYKKIAEYFEEASKGIKNK
                     KLASNYILGQIFRRMPTDSEKEECSISIPPEYLNELISLIENKKINSSMASNALEKML
                     DSGKPVREFISEKDMQGIDENTLREICLRALSENKEAVESYLGGKEKAFSSLIGYIMK
                     ETKGKADPQKAAEIIKEIIKA"
     gene            complement(6208..7665)
                     /gene="gatA"
                     /locus_tag="PLB33_06060"
     CDS             complement(6208..7665)
                     /gene="gatA"
                     /locus_tag="PLB33_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOG63122.1"
                     /translation="MSKIKYIQKLLKAKEISCVELTKNYLNNIEKQNKAINAYVNVTG
                     EEAIKTAIKVDEKIAAGEDLMPLEGIPMSLKDNISTKDIETTCCSKMLKGYYPIYDAT
                     VWEILRNQNSVLLGKTNMDEFAMGSSCETSIFGTTLNPHNTDYVAGGSSGGAAASVAG
                     NMAAFGLGSDTGGSIRQPASFCGIVGFKPTYGTVSRYGVVAYASSLDQVGPITDSVED
                     AAIVYDCLSLYDPKDSTSIGSKTGTCDKLHNSIKGLKIGIADEYFEGINEDIISSIEE
                     AIKVYQDAGAEIVNFEIPKLKAFLPVYFIIACAEASSNLGRFDGIRYGYKTDSYNDVN
                     DMICKNRSEGFGKEVQKRILLGTYALSSGYYDAYYKKAQNLRYSIIQDFKNIFKSCDV
                     IITPSVPVTAFKKGEALTDPIATYMMDICTVPANIAGLPAISVPCGFDKNKMPIGMQI
                     IGNSFEDYKVFNAAYQYEKQTKKSDEISPYPEVKI"
     gene            complement(7670..7963)
                     /locus_tag="PLB33_06065"
     CDS             complement(7670..7963)
                     /locus_tag="PLB33_06065"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOG63123.1"
                     /translation="MITKEEVLKIAKLAKLSVNNDEIDMLIEDMSKIIEYADAINTVV
                     EDEDNEVEFYNINNSANAFNEDIVIDSYDRDEILKNREGGEKGYFVIRKRQRG"
     gene            complement(7972..9720)
                     /gene="aspS"
                     /locus_tag="PLB33_06070"
     CDS             complement(7972..9720)
                     /gene="aspS"
                     /locus_tag="PLB33_06070"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG63124.1"
                     /translation="MLRKNEYRTHTCGELRESDIGKHVTVSGWLENVRDHGGVKFADL
                     RDQYGIVQVVVEDETMLDGIPKESAIRVTGFVAERDEDTINKKLDTGYIEIEAESITV
                     LGKVKEFLPFEVTSSKETKEDLRLKYRFLDLRNVEVQKNIILRSKVISFMRSKMTEMG
                     FLEIQTPILTSSSPEGARDYIIPSRKHPGKFYALPQAPQVFKQLLMVSGFDKYFQIAP
                     CFRDEDARADRSPGEFYQLDFEMSFATQEDVFSVAEEVLYDTFTKFSDKQVSKPPFKR
                     IPYEESMLIYGTDKPDLRNPLTIIDISDLFEETDFAPFRKKTVRAINVPECSSQPKRF
                     FEEMLEFATKIGMKGLGYIKVNDEMEYQGPIDKFLSNEDRKELAIRARLKPGDVLFFI
                     ADTREEAPKLAGQIRAELAKKLKLIDENRYEMCYIVDFPMYEIDEKTGGIAFTHNPFS
                     MPQGGLKSLMEKDPLDIKAFQYDIVCNGIELSSGAVRNHDLDIMIKAFEIAGYTIDDL
                     QTKFTSLFNAFKYGAPPHAGMAPGIDRIIMLLTGEENIREVIAFPMNNNAQDLLTNAP
                     SKVTEQQLREVHIKLR"
     misc_binding    complement(9762..10011)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10066..12504)
                     /locus_tag="PLB33_06075"
     CDS             complement(10066..12504)
                     /locus_tag="PLB33_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOG63125.1"
                     /translation="MMGGLNNSARNALALAQEEVKNFKQNVLGTEHILLGLMIEEESI
                     AGKILRDKLDIDKVREIIKNSTGMGDSVPEYITISPRSKFIIELARQYAAQLKMPYVG
                     CEHILLGLIEEGEGIGAQIIKSVISLKDLKNEVLMAMQGAGGAEPGNQDIGGSMAPQT
                     SNTKTIDKYGTDLNQRAKEGRLDPVIGREDVIERVIQILSRRTKNNPCLIGEPGVGKT
                     AIAEGLAQEIIKGSVPETLKDKRVITLDMAGMVAGAKYRGEFEERLKNAIDEIKAAGN
                     IILFIDEMHTIIGAGAAEGAIDASNILKPVLARGELQVVGATTLDEYKKHIEKDAALE
                     RRFQPIIVEEPTVEDTVRILKGLRDKYEAHHKVAITDEAIEAAASLSHRYISDRFLPD
                     KAIDLIDEAASRIRLKSSTAPKELKELEDKIKEVDTEKRTAINNQDFEKAASLRDEEK
                     KLTDELNKQRKLWAAKNIKDIKINYEDIADVVSQWTGIPVKKLQGDESERLLNMEEIL
                     HKRVIGQEPAVEALSKAIRRSRVGLKDPKKPIGSFIFLGPTGVGKTELSKALAEAMFG
                     DEDAMIRIDMSEYMEKHSVSKMIGSPPGYVGFDDGGQLTERVRRKPYSVILFDEIEKA
                     HPDVFNILLQILDDGRLTDAKGRTVDFKNTVVIMTSNVGASTIKKQKTLGFATNASDE
                     EKDEYEKMKENVLNELKKTFRPEFLNRIDEIIVFHSLNKEHIKQIVSLMINNLSKRLD
                     EMNIHIKVDEKAMDILADAGFDPVYGARPLQREIRRRIEDKLSEELLKGTIKKSDTIL
                     ISADDKELIFSNDK"
     gene            complement(12535..13500)
                     /locus_tag="PLB33_06080"
     CDS             complement(12535..13500)
                     /locus_tag="PLB33_06080"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOG63126.1"
                     /translation="MTSRIRLARNLKNYKLPIYMTESESNSVINEIYNAVSKHENYKL
                     TYMKDLSDVKKNSLIENHIISPALANKRNEGAFLLSPDGKTSVMINEEDHIRIQTIED
                     GLSLKECWKLSNEVDDIIEESVDYAFDRELGYITACPTNIGTGMRASVMLHLPALSIT
                     NQIDKLLYGVSQLGVAVRGVYGEGTKSIGHLYQISNQGTLGASEETLIDKINQIVMQI
                     IDKEKNTREHLMKNNYDDLEDDFCRAYGLLTNARKMTVDEAMKLLSLIKLGSEMGITP
                     MVKGKNIYQLMIWIQPNNISTIDAELSPKDRDKKRAEIIRRELLR"
BASE COUNT         3563 a         2849 c         2006 g         5083 t
ORIGIN      
        1 tctgacagta cccatagttc ttttccaata ttgctctttt aatacctggg atgcatgaag
       61 ttcttcaaaa tcggcacttt gttccggtgt tttcacccac atttccaatt cggtggcagc
      121 agttagcaaa acctcatcaa tgtcttcata tttaaaccct aaggattctg caatatgggg
      181 atgatttttc aaaacatttt ttatttcatt tttgaaatga acttcggctc ttctaagtgt
      241 tgacctggag cacactaatt tgtttttgtg gacaataaaa gaaggaatta ttaaagttcc
      301 gacaggaaga ccattttcat cattacaata ttcataatta tagtcaacaa accatgtaca
      361 gtctgtatcc ggcagcaaat caacccttgc atcgcttaaa tcagctatac cctgcagctc
      421 aacgctggag ccgtctgtct gtatgccgta tttaagcatt tcttccatat ccttaataaa
      481 cagttttaca ggtatttttt cgtctgttcc gtttccgcct atatcgacac ccataaagga
      541 tacaaattgt atttcattat gctcgcttaa aattttggtc aaattttcaa catcgtgata
      601 ttcagctttt attgtataaa ttctgctcat atactctcct tctagccctt gcatatatct
      661 ataagctcag agggctcaaa tattaaataa tcaggttcat gttttctaag gctttcaacg
      721 gtattataac cccatcctac cgatgctata tccattcctg catttcttgc agcagtaata
      781 tctcttattt catctccaac ataaagaaat ttatccagac tcatttttgc ttttcttgct
      841 attttcttca tttttgattc ttttccaaac atggcagcgc tgacaattgt atcaaaaaca
      901 tcaacattgt gcgtttctaa aaaaagctta acatttgttt tagaattagt tgtaataatt
      961 cccgttttta tgcccatatt tcttagtctg cctataatat gaaaaatatc aggcttacaa
     1021 agacttatat tcttaatatc ttgagttaat aaggtttttc ccctctttaa cagaaaaggc
     1081 agatatcttt tttttaattg aatatatgcc ataacttcct tagcatgcat ctttttcaga
     1141 cggcctaatt catccatggt aatatttctt aaattatatt tttctgctaa cttttgatat
     1201 ataacgaaat ttgcttcttc agtatcagct aatgttccat caaaatcaaa aataacatat
     1261 ttatatttca ctgacaccca tccttaagta tataaatttt atttattact atattatatt
     1321 ttttttaatc agtcaattta aaattaaaaa aactcctttc ggagtttttt acaatgattt
     1381 aattattttg ccgcagcctt ccctgataaa actcatgtac tgctcctctt taataaggcc
     1441 tgttattact tcatcaggta ctgtttcatt tagatattcc aacaactttt tcttggagtt
     1501 atctatattg cttgctgcaa atatataaga ttggacagcc tttatatctt ctgtatattt
     1561 ttcataaaaa ctgccgatag cttttttgtc ataatagttt aaaagatttt taactgcaga
     1621 gtttaattct tcacctttta aattttgctt attatgtatt ttaagcttgc ttgcattaaa
     1681 tttgtaatac tcatcgagcc aaagatggca ataataacct aagacaatgg agctgtcctt
     1741 taagctcatg gttttaaaat caaatttctc ataaaactcc cttacatcta tattcccgtc
     1801 ttcatcaaca ttgtgcagct cttctaactg gtcatcattt aatgtacttg gtaaaattat
     1861 ccctataata aagttaggta tatcaacttt atcgtaactt tccataaact ttttcccaaa
     1921 gtgcattacg gtactgatag gcggcatttg tactttttac tccttttcaa ttgttaatcg
     1981 cagcggggat attctgtcgg taagtccccc tcgctacagg gatattcctt cggccatcga
     2041 agactttccc ttgagtagat tgtatccctc ttcctagact attatatcaa acacaaatca
     2101 agatgtacag aattaagtta atgaaacttg cggcgggtct gtgatttcac ctgtttttgg
     2161 atttacaaaa gcaagccagt atccgctttc tcctttattg tctttatttg catatgacca
     2221 atttttaaat ccggtcacac ctttataagg ctgctcttcc tttgtaaatt ctccgggcac
     2281 cccgtatata aaccgtgcaa tctcgtcgtt ttccttgtag atgccgaaaa tataatgccc
     2341 gtattttttc attagacttg tgcaggtagt cacgcgatta gacatgggat acggataata
     2401 tgcgcttatt atttgattgt aataaggcaa tactcctgtt tttatgccca tttcgtcata
     2461 aggtatatac caccaattaa aattctttaa tttaaccgat aaaggttcaa tttctttaaa
     2521 ttctctcaat gttttataca atttctcatc aaaacggttt ctataaccct gattggaaat
     2581 atcatctttt ttgcttgatt tttccggagt ctccatggtt tcttcactat cttcttttat
     2641 tgtttcttta acttttattg tttttactgg ttcttgtact acttttttta cttcttcttt
     2701 tgtttcatct tccggtttat tttctgctgc ttttgccatt cccttctctc ttatttccct
     2761 tacctctcgt aatccttttg gctcgattat ttcttttgtc tcaattactt cttttggttc
     2821 tatcgctccc cttgtatctt ttatatttat ctgctctttt tctttagtat ggataggctt
     2881 tgactcttga aatttttgtt tcttatcggc aaaaatatct ttttcctgct cattatcatc
     2941 ttcttccaac tttctcacga tgtcttggct tgatacgctg aacaaactgg aatataaaac
     3001 aatttttcca tcctttacaa cggcgcatac gttgtaatct tcaattgcat tgctgttttc
     3061 aaaggtgagc ttctttttga ttataccttt tttattgtta atatctccta atttgatttt
     3121 gttgtgttta tccttatata aataaacttc acttgatgat tttgcatcac ctaaattttc
     3181 agcagctaca atcatgtttc ccctgttccc tctcatgtcc agacgtacat agcctctaga
     3241 cttgtctttt ttatttaccg gctcaaaaaa cttcaacact ctataatttt tattgttcat
     3301 aaaaacctcc taatatctct gttatttatt tatatgctga tttttttgta taattacaga
     3361 tatggcaagc tttaatcaaa caagcttggc aatatcatat aaactgctca ctaaaagcca
     3421 gttctgcggg aaatatctca ttacgttcca atacccgaca ccttcaaacc cgtattcact
     3481 gaataaatcc aattttgcca agatgcttct tgcatcttca aaccaaactc tatgctgcct
     3541 tccctgttca tcataataaa taaagtaggg agcttgtgat acttcatcat actgaatgta
     3601 tgctccaaca tcagctgcca aatcaactgc ctcaacattg gaaacggagt ccgctcttga
     3661 tacaccttga acatatggca gtataaaatc ataaccgtag ttaggaatgc ccatataaat
     3721 tttttccctc gggatttcag tcactgcata atctaacact tcccttaccc tgtcaacggg
     3781 agatacagcc attggaggac cataagtata accccattca taagtcataa gaagaactct
     3841 atctgacgct tctccgattc tcggataatt atgagcttca tagagaagtc ccggctggtc
     3901 tgctgatgtt tttggtgcca acgccgtaaa taaaacaaat ccttcagggc ttattctgtc
     3961 atgaacattt tgaataaaat ttataaaagc ttcttcgtcc tccggcagga catattcaaa
     4021 atctatgtct agtccgtaat aacctttttc tcttaaattc tgcaatacat tttcaattaa
     4081 attgttttgt gcaaccggat catttaaaat aacatgtgcc agttcattgc tgaaagtccc
     4141 ttcttcagtc aatgtggaaa taagcataag cggtgctaca ccaaaatctc ttgccattct
     4201 gattatgtct tcgtcttcta tatcgattaa ttcgccttct tcagtgattc cgtaagtaaa
     4261 tatcgtcaga taagttaagt aaggaagtgt tttcaaaagt actgtcctgt ctatatgcgg
     4321 ataagcatat ccaagcacat gtatgagacc tcttttatct gctgtattgc ttattgtaat
     4381 ttggtcacct ggcacaattt gatctgcata ttcaataaag ggattaagct gcagtaaatt
     4441 tacggtggtt gtattgtatt gccttgctat atctgccaat gtttctcccg gctgtacaac
     4501 atgaattaca tccggaataa gtattactaa ggtctggccg ggtacaagga cattagggtt
     4561 ttcaagttcg ttatctataa ttattttttg cggtgatata ttgtattgat ttgcaatgga
     4621 atatattgtt tctccgggct gtacaacatg aattatcata aatattctcc tttctgttct
     4681 ctcattagta tatgcatgaa aatttcaatt gttatgaaga aatgtcgggc tataacaaac
     4741 aaaaaacaca tcacaaggaa tcttatgatg tgttttagtc attgatgagt attcatcaag
     4801 cctttattat ctcttttatg atttctgcag ctttttgcgg gtccgctttt ccttttgttt
     4861 ctttcattat atacccgatt aaggagctga atgctttttc tttccctcca aggtaggatt
     4921 caaccgcttc tttattttct gaaagggctc tcaggcagat ttctctgaga gtattctcat
     4981 ctataccctg catatccttt tcggatatga attccctcac cggttttccc gaatcgagca
     5041 ttttttcaag agcattgctt gccatgctgg aattgatttt tttattttca atcaatgaaa
     5101 tcagctcatt taaatactca ggcggaatgg agatggagca ttcctctttt tccgaatcag
     5161 tgggcattct tctgaatatc tgccctaata tataattgga tgctaatttt ttatttttta
     5221 tacctttgct tgcctcttca aaatattcgg caattttttt gtattttatc aaaagctccg
     5281 catcatcctc cggaagactg tattcattaa taaatacatg caatttttca tccggcagct
     5341 gaggaagttc actttttaat ttttttattt cttcttcgga aactattata tcgactaaat
     5401 caggctcttt gaaatacctg tagtcttcgg catcttcttt tcctctcata ctttcggtaa
     5461 tattaagctc ggaatcatat cttctcgttt cctgctcaat ttcaccgccg ctttctaaaa
     5521 tgtccacatg cctttcaaat tcatattcca ttgctttggc tataaagctg agggagttca
     5581 tatttttaat ctccgtcctg gtgccaagct tttcttctcc ctttttcctt accgatatat
     5641 taacgtcaca tcgcagtgag ccttcctgca ttttgacatc cgatacgcct atatacttca
     5701 taaccagtct taacttttcc aaatactcga gaacttcatc aatgctgcta aaatcgggct
     5761 ccgttacaat ttcgataaga ggtactccgc ccctgttgta atcaatataa gtatttcctc
     5821 tttcatggac taactttcct gcatcttctt ctatgtgtat cctgttaata ttaatatttt
     5881 tgcctgatga aagtttaata tatccaccat aacaaatagg tttatcaaat tgagatattt
     5941 ggtatgcttt tggtaaatca ggatatacat aatttttacg gtccattttt gaaatatttc
     6001 ttattttgca gtttaaggca atgcctgctt ttacggcata ttccacaacc tttttattaa
     6061 gtttgggaag agtgcccggc attcctatgc atatgggaca gcaatgagtg ttaggctccc
     6121 ctccgaactc tgtcgtgcat tgacagaata tcttagtctt agtagatagt tctatatgtg
     6181 tttctaatcc tgcaactaat tcataactca tattttcacc tccggatagg gggatatttc
     6241 atctgacttt tttgtttgct tctcgtattg atacgctgca ttaaacactt tataatcttc
     6301 aaagctgttg ccgattattt gcatccctat gggcatctta tttttgtcaa agccgcatgg
     6361 aaccgaaatt gcaggtagac cggcaatgtt tgccggaaca gtacaaatat ccatcatata
     6421 agttgctatg gggtcggtta aagcttctcc ttttttaaat gctgtcaccg gcactgaagg
     6481 agtaataata acatcacaac ttttaaatat atttttaaaa tcttgtataa ttgaatatct
     6541 taaattttgc gccttcttat aatatgcatc gtagtatccg gaacttagcg cataagtacc
     6601 aagcaaaatt cttttttgca cttccttgcc gaaaccttca ctcctgtttt tgcatatcat
     6661 atcatttaca tcattgtaac tgtctgtttt atatccgtat cttataccat caaatcttcc
     6721 taaattagat gaagcttcag cgcacgcaat aataaaataa accggaagga aagctttcag
     6781 cttaggtatt tcaaaattta ctatttctgc acccgcgtct tggtaaactt ttattgcttc
     6841 ctctattgaa cttataatat cttcatttat tccttcaaaa tactcatctg ctattcctat
     6901 ttttagtccc ttgatactat tatgtaattt atcacatgta ccggttttgc ttcctatgga
     6961 tgttgagtcc ttagggtcgt ataaggacag gcagtcgtat actattgcag catcttccac
     7021 agaatcggtt attggtccca cctggtccaa gcttgaagca taagcaacaa ctccatatcg
     7081 ggaaaccgtg ccatacgtag gtttaaatcc tacaatgccg caaaagcttg caggctgcct
     7141 tatggaacct cctgtgtcgg agcctaagcc gaatgctgcc atattccccg ctacggaagc
     7201 tgcagctcct ccggagcttc ctcctgccac ataatccgtg ttgtgaggat tcaatgttgt
     7261 tccgaagatt gaagtttcac aagatgagcc catggcaaac tcatccatat tggtctttcc
     7321 cagaagcacg gagttttgat tccttaatat ctcccaaaca gtagcatcat aaataggata
     7381 atatccttta agcatttttg agcagcatgt tgtttcaata tccttggtag aaatattgtc
     7441 ttttaaagac atggggatgc cttccaaagg cattaaatct tctccggctg caattttttc
     7501 atcaaccttt attgctgttt taattgcttc ttcacctgtt acgttgacat atgcatttat
     7561 agccttattt tgtttttcaa tgttgttaag gtagtttttt gtcagttcaa cacatgaaat
     7621 ttcctttgct tttaataatt tttgtatata ttttattttg ctcaattttt caccccctct
     7681 gtcttttcct tattacaaaa tatccctttt ctccgccttc tctgttcttt aaaatttcat
     7741 ccctgtcata agaatctata acgatatctt cgttaaaagc atttgcactg ttgttaatgt
     7801 tataaaattc aacttcatta tcttcatctt caacaactgt attaattgca tctgcatatt
     7861 cgattatttt agacatatct tcaatcagca tatcaatttc atcattattt accgataatt
     7921 tagcaagctt tgcaattttc aaaacttctt ccttagttat cattatctca cctacctcaa
     7981 ttttatatga acttctctta attgctgctc tgtaaccttt gatggtgcat ttgtcagtaa
     8041 atcttgagca ttgttgttca tggggaaagc tataacttct cgaatatttt cttcgcctgt
     8101 aagaagcatt attattctgt caattcccgg agccatgcct gcgtgaggag gtgctccgta
     8161 tttaaatgca ttgaacaagg aggtaaattt tgtttgtaaa tcatctattg tataccctgc
     8221 tatttcaaat gctttaatca ttatatccaa atcgtgattt cttacagctc cggatgaaag
     8281 ttcaataccg ttacatacaa tatcatactg aaaagcttta atgtcaagag ggtctttttc
     8341 cattaatgat ttcaatcctc cctgaggcat tgaaaacggg ttgtgtgtaa atgctattcc
     8401 tcctgttttt tcatctattt catacattgg aaaatcaaca atataacaca tttcatacct
     8461 gttttcatct attaatttca gtttctttgc aagctcggct cgtatttgtc ccgcaagttt
     8521 aggcgcttct tctcttgtat ctgcaataaa aaacaatacg tctcccggtt tcagtcttgc
     8581 tcttatagca agttcctttc tgtcttcatt agaaagaaat ttatcaatgg gaccctgata
     8641 ttccatttca tcattaacct tgatgtagcc aagtcccttc attccgattt tagttgcaaa
     8701 ttcaagcatc tcttcaaaaa aacgcttggg ctgggatgag cattcaggca cattgattgc
     8761 tcttaccgtc ttctttctga aaggtgcaaa gtctgtttct tcaaacaaat cgcttatatc
     8821 aattattgtc agcgggtttc tcaaatccgg tttgtcagtg ccgtatataa gcattgattc
     8881 ttcgtaagga atccttttaa aaggaggttt tgatacttgc ttatcggaaa acttagtaaa
     8941 ggtatcatac aatacttctt cagcaaccga aaatacatcc tcttgagttg caaatgacat
     9001 ttcaaaatcg agctgataaa actcaccggg agagcggtca gccctggcat cctcatctcg
     9061 gaaacaagga gctatctgga aatacttatc aaaccccgaa accatcaaaa gctgcttaaa
     9121 cacttgagga gcttggggca atgcataaaa ttttccggga tgctttctgc ttggtattat
     9181 atagtctctt gctccttcag gagaagaaga tgttaaaatc ggtgtttgaa tttccaaaaa
     9241 acccatttcc gtcattttgc ttctcataaa ggatattacc tttgagcgaa gaataatatt
     9301 tttttgaact tccacgtttc ttaagtccaa aaacctgtat tttaatctta aatcttcttt
     9361 tgtctctttt gatgatgtaa cttcaaaagg aagaaattct ttgaccttac ccagtacggt
     9421 tatagattct gcttcgattt caatatatcc cgtatcaagc tttttattta ttgtgtcttc
     9481 atctctttca gcaacaaaac cggtaacacg aattgcactt tcctttggta ttccatccaa
     9541 cattgtttca tcttcaacaa caacctgtac aatgccatat tggtctctta aatctgcaaa
     9601 cttaacacct ccatggtccc tcacattttc aagccatcct gatactgtga catgctttcc
     9661 tatatcgctt tctcttaatt ctccgcaggt gtgtgtacgg tactcatttt ttcttaacat
     9721 aattattaat cctttcatat aaaataaaaa ctcctccatc cacaagggac gaaggagttg
     9781 aatttcgcgg tgccacccta attagcatat acttacatat aggctctctc aaaagacact
     9841 gacatgtctt tgccgcttta tcgctcggat gcgtttgagc ctactcttgc atatgcaatt
     9901 tgggtcaaaa gctcagggat gttcttcgtt tataatcttg taatagactc tcaccggcgc
     9961 tatctctctg tgactctaat tataaattac tcttcccatc attgctgagt attttctaca
    10021 tttttaatta ttatatacaa actacaactt ttgtcaagag tttttttatt tatcgttgct
    10081 gaaaattaat tctttatcat ctgctgaaat taaaatcgta tctgacttct tgattgttcc
    10141 ttttagaagc tcctcagata atttgtcttc tattcgtctt ctgatttctc tttgaagagg
    10201 tcttgcgccg tataccgggt caaatcctgc atctgctagt atatccattg ccttttcatc
    10261 gactttaata tgaatattca tttcatcaag acgttttgac agattgttta tcataagtga
    10321 tacaatttgt tttatatgct ccttgttcaa tgagtggaag acgattatct cgtcaatacg
    10381 gtttaaaaac tcaggtctga atgttttctt caattcattc aatacatttt ccttcatctt
    10441 ttcgtattca tctttttctt catctgaagc attagttgca aacccgagtg ttttttgttt
    10501 tttaatggtg cttgctccca cattggatgt cataataaca actgtgtttt taaaatctac
    10561 cgtccttccc tttgcatcgg tcaaacggcc gtcatcaagt atttgaagca agatattaaa
    10621 tacatccgga tgtgcttttt ctatttcatc aaacaaaatt accgaatatg gttttcttct
    10681 tactctttcg gttaattgac ctccgtcatc aaagcctaca tatccgggag gagagcctat
    10741 catttttgat accgaatgct tttccatata ttcggacatg tcaattctta tcattgcatc
    10801 ttcatcacca aacatggctt ctgccaaggc tttcgagagc tctgtttttc caactcccgt
    10861 aggtcctaag aatataaatg aaccgattgg tttttttggg tctttaagtc ctaccctcga
    10921 gcgtctgatt gcttttgaca atgcttcaac agccggctcc tgacctatta ctcttttatg
    10981 aagaatttcc tccatattca ggagtctttc ggattcatct ccctgaagtt ttttaacggg
    11041 gatacctgtc cattgggata caacgtcggc aatatcctca taattgattt ttatatcctt
    11101 tatgtttttt gcagcccaaa gttttctctg tttattaagt tcatccgtca gcttcttttc
    11161 ttcatctctc agcgatgctg ccttttcaaa atcctgattg tttattgcag ttcttttttc
    11221 agtgtcaact tctttaattt tatcttccaa ttcttttaat tccttgggag ctgtcgatga
    11281 ttttaatctt atccttgaag ctgcttcatc tattaagtct attgccttgt caggcaggaa
    11341 tctgtcggaa atatatctgt gagaaagact tgccgccgct tcaattgctt catcagtgat
    11401 tgccaccttg tgatgagctt cgtatttatc tcttaatccc ttaagaattc ttacggtgtc
    11461 ttcaactgtc ggctcctcaa caattatagg ctggaatctc ctttcaagag cagcgtcttt
    11521 ttcaatgtgt tttttatatt catctaaagt agttgcacct acaacctgaa gttcccctct
    11581 cgccaatacg ggttttaaaa tattggatgc atctattgcc ccttcggcag ctccggcacc
    11641 gattattgta tgcatttcat ctataaataa tataatattt cctgctgcct ttatttcatc
    11701 gatggcattt tttagtcttt cctcaaattc tcctctatat tttgctcctg ccaccatccc
    11761 tgccatatcc aaggtgatta cacgcttatc cttcagtgtt tcaggaacgc ttcctttgat
    11821 aatttcctga gccagacctt ctgcaattgc cgtctttccg accccgggct caccaataag
    11881 gcaggggttg tttttagttc ttctcgataa gatttgaatt accctttcaa taacatcttc
    11941 tcttccgata actgggtcaa gccttccttc tttcgcacgc tgatttaaat cagtgccgta
    12001 tttatcaata gtcttagtat ttgatgtttg cggtgccata ctgccgccta tatcctgatt
    12061 gccgggctcc gctccgcctg cgccttgcat tgccatcagc acttcatttt ttaaatcctt
    12121 taaacttatc actgatttta taatttgtgc tcctatacct tcgccttcct caataagtcc
    12181 caataaaata tgctcacaac ctacataagg cattttaagc tgggctgcgt actgacgtgc
    12241 cagctctatt ataaatttgc ttctggggct aatagtaatg tattcaggta cagaatcacc
    12301 catgcctgtt gaatttttaa ttatttctct aactttatca atatctaatt tatctctaag
    12361 aatttttcct gcaatacttt cctcttcaat cattaaccct aatagaatat gctcggttcc
    12421 aagtacattt tgtttgaaat tttttacttc ttcctgagca agggctaagg catttcttgc
    12481 agaattattt agtcctccca tcatatcttt catctccttt ttgtattata tatttcaacg
    12541 caataattca cgtcttatta tttctgctct ttttttgtct ctatcctttg gtgaaagttc
    12601 agcatcaata gttgaaatgt tgtttggctg aatccaaatc ataagctgat atatattctt
    12661 gcctttaacc attggagtta ttcccatttc gcttccaagc tttatgagcg acaataattt
    12721 cattgcctca tccactgtca tttttcttgc atttgtaaga agtccgtatg ccctgcaaaa
    12781 atcatcttcc aaatcgtcat aattgttctt catcaggtgc tctcttgtgt ttttttcctt
    12841 atcgataatt tgcattacaa tttgattgat tttatcaatt aatgtttctt cggaagcacc
    12901 caatgttccc tggtttgaaa tttgatacaa atgtcctatc gattttgttc cttcaccata
    12961 tactcctctc actgcaacac ctaactggga aaccccgtac aggagtttgt caatttgatt
    13021 tgttattgaa agtgcaggca aatgcagcat tacagatgct ctcatacctg ttcctatgtt
    13081 agtggggcag gcagttatat atcctaattc cctgtcaaat gcataatcaa cactttcctc
    13141 aattatatca tccacttcat tgcttagttt ccagcattcc ttcaaggata atccgtcttc
    13201 tatagtttgg attcttatgt gatcttcctc atttatcata actgaagttt ttccatcggg
    13261 gcttaaaaga aatgcacctt cattcctttt atttgccaat gccggagaga taatatggtt
    13321 ttcaatcaat gaatttttct taacatctga taagtctttc atatatgtta atttgtaatt
    13381 ttcatgttta cttactgcat tgtatatttc attaattaca ctgtttgatt ctgactcggt
    13441 catatatata ggtaatttgt aattttttaa atttcttgca agtctgattc tgctggttat
    13501 t
//
LOCUS       DAOSHP010000050        13237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3923_length_13237_cov_9.387574, whole genome shotgun sequence.
ACCESSION   DAOSHP010000050 DAOSHP010000000
VERSION     DAOSHP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_13237_cov_9.387574"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..1199)
                     /locus_tag="PLB33_06085"
     CDS             complement(315..1199)
                     /locus_tag="PLB33_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOG63127.1"
                     /translation="MEFKEWFKEKVSIVDEYLGQIIEVKENPQKIIYQAVNYSLLSGG
                     KRLRPVLFLGAYELFSDDLKKALPFACIMEMIHTYSLIHDDLPAMDNDDYRRGKLSNH
                     KKFGEARAILAGDALLNKAFETGLDAAINFKGMNAIKALKLIADSSGTEGMIGGQVID
                     IDGENKISSIEDLKYMYSLKTGAIIKSSVTAGATLAGATENEIKLLENYAEKVGIAFQ
                     IMDDILDVTSTQEKLGKLIGSDAANNKTTYLTFKSIDEARKDVEKFTEEAIKSLSLFG
                     NRAIYLIELAKYLTSREN"
     gene            1559..3268
                     /locus_tag="PLB33_06090"
     CDS             1559..3268
                     /locus_tag="PLB33_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63128.1"
                     /translation="MKMRKIFALFLALLMVALVFAGCTQTSQPSAPQADTQPPSEEEQ
                     QADEAKSEQILRYGASGFNGIFNPIMFDNVYDGYISNIIFEKLITHTPEGEYIPGIAE
                     WKLSEDKLTYTFTLKNGIKFSDGTDLTAEDVAFTYMTIAHPEYNGPMAYAVSNFEGYD
                     EYHEGVTDEFEGIKVIDEKTISFTFKDGRAAPANIECFDYGIMSSDYYAFENWEDFLA
                     LIDKPMGSGIMTLDSWEPKQFILLKKNTNYWDPENGAKIDSLLVSEVPEESILSALQT
                     NQIDFAQISPSAENLEAAKAMDNINIVNYIGNGYTFMCFNTTRPALEDVRVRQALMHA
                     LDRESFIKVQYGEGLAAVGMAPISPASWAFPDLSELNPYSFDMEKAAELMDEAGWLME
                     SDGYRYKDGQKLAVNWLVFTEADWPKTLYEMAADTWKQLGVDLSMELMDGNSVIARTI
                     GPEPGEKDFDIYTLAFTLTIDPDPTGFLFDDDAYMAGGFNASGYKNAQAMDLVRAGKK
                     EFDIEKRAEIYKEWAKIMNEEIPHAIIAYRSEIWGINKRVDGMFIDTYATWDLSLKNV
                     TLK"
     gene            3337..4302
                     /locus_tag="PLB33_06095"
     CDS             3337..4302
                     /locus_tag="PLB33_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63129.1"
                     /translation="MRYYILRRIIQIIPVLIGIIFILFFILEQAPGTPVSYMMHPRMT
                     PEQKAELEEKLGLDIPWYERFVNYIKEASQGNLGYSSTHKKPVTEVIGNYAGPTLLLA
                     LVSLTISIFIGIPAGIISAVKQHTVIDNILTVMSFIGISIPSFFFGLLLLKAFAVDIQ
                     LFPLFGLKDPLLMSDNIFVKIKDIAWHLVLPSIVLGLNSTASFMRYTRSSMLEVIKQD
                     YIRTARAKGLKEKTIIYRHAFRNGIIPLITLLGFWIPLLLSGAIVTESIFALPGLGKI
                     SVEAAMSRNYPLILGINSILAILTLIGSLVADILYALADPRIRYD"
     gene            4313..5197
                     /locus_tag="PLB33_06100"
     CDS             4313..5197
                     /locus_tag="PLB33_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63130.1"
                     /translation="MAKDKTNNNYTGNQSYWGQVLSRLRKNKMAMISLYILIIIIALC
                     IIIPIISPYEIQTTNLSGKDQKPNSRHLLGTDLIGRDIFTRLFYAGRISLGIALATVI
                     IECVIGVVLGSLSGFYGGIIDSIIMRITEMFMSFPFMIICITVVAVFGNSIPNLILVL
                     AILSWPNIARIVRGQILVLREMEYMEACEALGISDLRRIFKHLLPNVSAYVIVYATLG
                     MASVILVETSLSFLGLGVSPPTPTWGNMIQEARNILIIQQKWWYWVPPGTAIFISVMC
                     FNILGDGIRDALDPKMMR"
     gene            5202..6176
                     /locus_tag="PLB33_06105"
     CDS             5202..6176
                     /locus_tag="PLB33_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63131.1"
                     /translation="MDKKLLEVKNLKTYFYSDGVIIKSVDDLSFYVNEGETLGIVGES
                     GCGKSVACLSLARLVETPPGKYEYGEILFKGEDLLRVSDARIRQIRGNDISFIFQEPM
                     TSLNPVFKIGRQISEVLILHRGISKEEAYEESIRMLELVKIPNPERVVNDYPFALSGG
                     MRQRVMIAMALACKPGLLIADEPTSNLDVTIQAQVLDLMNELKEKINTSIIFITHDLD
                     VIAEMSDRVMVMYAGKVVEEASTNEILKNPRHPYTVGLINSKPNMAKEGTRLNVIPGN
                     VPDLSNLPPGCSFHPRCDKAMDICKRVIPDEKLLYEGRHRISCWLYAD"
     gene            6187..7161
                     /locus_tag="PLB33_06110"
     CDS             6187..7161
                     /locus_tag="PLB33_06110"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOG63132.1"
                     /translation="MSEYLITVNNLKKYFPIKKGLVKRTSGNVKAVDGVSFNIQRGQI
                     LGIVGESGCGKSTMGRTILRLLPPTGGEVFYEGKNVFNMSKREMNKMRTKMQIIFQDP
                     YSSLNPRLPVYDIVGEAMLQHGIVKNKKEMKEKVIEIIGKCGLSPEQADRYPHQFSGG
                     QRQRICIARALAVNPDFMVCDESVSALDVSIQSQIINLLKDAQEDYGLTYMFISHDLS
                     IVKFISDEIAVMYLGQIIEKGSKKQIFSNPLHPYTEALLSAVPSLKQNKRRIVLNGDI
                     PSLVNPPKGCRFYTRCPYVKDICKEEIPEYREINTGHFAMCHKVNGVF"
     gene            7161..7394
                     /locus_tag="PLB33_06115"
     CDS             7161..7394
                     /locus_tag="PLB33_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63133.1"
                     /translation="MFLKKNRVDEDLERIREANLPNKEEIKMNAKHSDDLSLEKGDLL
                     AMVLAVISLILPYVIAFLGAIGVVIFFMYLFFS"
     gene            complement(7481..7681)
                     /locus_tag="PLB33_06120"
     CDS             complement(7481..7681)
                     /locus_tag="PLB33_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOG63134.1"
                     /translation="MAQGTVKWFNSEKGFGFITKEDGNDVFVHFSAIQGDGFKTLEEG
                     QRVSFDVTQGNRGDQAANVQRI"
     gene            complement(7949..8998)
                     /gene="ftsZ"
                     /locus_tag="PLB33_06125"
     CDS             complement(7949..8998)
                     /gene="ftsZ"
                     /locus_tag="PLB33_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOG63135.1"
                     /translation="MFQFEEPSERLANIKVIGVGGGGNNAVNRMIDAGVRGVNFISIN
                     TDKQALGLSKAETKFQIGEKLTNGLGAGSKPEIGEKAAEENKQDIAELVEGADMVFIT
                     AGMGGGTGTGAAPIIANIAKSMGILTVAVVTKPFMFEGRCRMRNAEMGLKRLKDHIDT
                     LVVIPNDKLLEITDKKTTMLEAFAMADEVLRQGIQGISDLIAIPAIMNLDFADVETIM
                     SGQGIAHMGIGMASGENRALNAVKQAIQSPLLETSINGARGVLVNITGGPSMGIHEVN
                     EAAKLIEEAADPEANLIYGANIDESAGDTIKITVIATGFADGAKKSDGEPSEKSEKKP
                     ENPFDFTIPSFLNKK"
     gene            complement(9134..9478)
                     /locus_tag="PLB33_06130"
     CDS             complement(9134..9478)
                     /locus_tag="PLB33_06130"
                     /inference="COORDINATES: protein motif:HMM:NF018632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HOG63136.1"
                     /translation="MIYVAIGLILGIVAGLNLNFIYNPDYIVYISLAILAILNTIINI
                     LHENKTSELTITKSIAFLLTDLLFALFLGFVGEQLGLPIYLAAVFAFGNNIYKKIKVI
                     VDIYLEKYFINK"
     gene            complement(9475..10206)
                     /locus_tag="PLB33_06135"
     CDS             complement(9475..10206)
                     /locus_tag="PLB33_06135"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOG63137.1"
                     /translation="MSTIKKTLNIIIFVLVFLTAAAIVNGADAGIDEYTIYTIRDLNK
                     YAHSIKEMKLLVNNMRNAVESEKRTIADYESKKYLDDIIEIKSQIEQIKLYNGFTAVR
                     GPGIMLRVSDNINEDYDLELMERIVHDVDIVVLLNDLKAAGAEAIEVNGKRIINISEV
                     VCAGPLIRVNGEVIPAPFVIIAIGDMDELYDAVTEEGTYAYELKKTYGMEVVVAQGYN
                     LTVREFRGINYETKYAEIVEEGEKK"
     gene            complement(10210..10926)
                     /locus_tag="PLB33_06140"
     CDS             complement(10210..10926)
                     /locus_tag="PLB33_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOG63138.1"
                     /translation="MKNKIWQKVMMFSISLILGIILVTQMRTKSNILGTVNLDKKAVE
                     LNEELAKLSIEKNRLRDELESLKKESRESEELFKDAEEELLKLKEELKVQKIISGYYD
                     VKGPGTIITIDAEPDTYLSLASSHQYILALISYLNNAGVEAISINGQRYTNYTEIVPV
                     LEHINVNGVAMVLPLEIKAIGDRSTINSSLNFVGGIVSQLNEIGYIIETEDSDEIFIP
                     RYDGEKQFKYALPIIRDEEI"
     gene            complement(10937..11719)
                     /locus_tag="PLB33_06145"
     CDS             complement(10937..11719)
                     /locus_tag="PLB33_06145"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOG63139.1"
                     /translation="MDENNNKKTQRKKKRRRNKKLMVSTAAFITAAVVVVFCICYVYY
                     KTPYFNLIGVDIDGNLTYSDEYIMEKSGIEVGKKIFEVDRSKVKENIENEVYVENARV
                     VYELPNKIFLDIKERVDTYQVFFNNEYIIIDKNGIVLRTCNEKLDLLTIESYTDVVYN
                     IGDNIQFAGVENNEKVFNAIEYLNNEYGSDIIKAFNIELNNSFLFKTQYGTAVRIDLS
                     EDINYQIVFGMKIINERLNNNLTVATGLIDFTKGDSPVYIED"
     gene            complement(11933..13192)
                     /gene="murA"
                     /locus_tag="PLB33_06150"
     CDS             complement(11933..13192)
                     /gene="murA"
                     /locus_tag="PLB33_06150"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOG63140.1"
                     /translation="MGSFIVEGRNSLEGIVDIGGSKNAALPILASTVITGREYILENI
                     PDIEDVKDMLEILSNMGCSVQFENGIALIDTRGLNTHIVPEKLVRKIRSSIILMGAVL
                     QRTGKVEVAYPGGCEIGLRPIDLHLKGLRKLGVEITEKNGLLVGSVNEALSCDIHLDY
                     PSVGATENIMLFAVGNPGKTTIYNPAREPEITDLQNFLNTCGFCVSGAGTGIITIEGK
                     KVQNTDTVEYKIISDRIEAGTYLSAAASMKSKIYIRNIEKSHFKSITSLYEDAGCEIR
                     ETHGTIVIANNKRIKAIEKITTQPYPGFPTDMQAQVMASMTVADGTSIIEETVFDSRL
                     KHVPELQKMGANITTIGRVAVVEGVEKLYSAEVEAKDLRGGAALVIAAMVAEGTSKIN
                     NIHHIKRGYEKFIYKLQNLGVIITEIE"
BASE COUNT         3982 a         2508 c         2323 g         4424 t
ORIGIN      
        1 aaaatgaaga attattcaaa agatgcaatc tatgcgggaa gcttccggga agctgcggat
       61 atagtaaggt caatggcaaa aaaaggtgat ataattatca ccatgggtgc cggaaatgtg
      121 gttgatatag ctgatatgct gattgcagat agataagtgt ttcatcctga gcgtaagcga
      181 aggatctcgg gttttataag atgagattct tcgagctaaa gctctcagaa tgacacgtag
      241 cttattcata gcaatgacga gagctgtgcc ctcggttgac agggcgtcat cctgagcgta
      301 agcgaaggat ctcattagtt ttctctgctt gtgagatatt ttgccaattc aatgagatag
      361 attgctctat tgccgaatag gctcaagctt ttaattgctt cttccgtgaa tttttccacg
      421 tcctttcttg cttcatctat acttttaaat gtaagatatg ttgttttatt gtttgctgca
      481 tcacttccga ttaactttcc cagtttttcc tgagtacttg ttacatccaa aatatcatcc
      541 attatttgaa atgcaatgcc tactttttcg gcgtaatttt ccaatagttt tatttcgttt
      601 tctgttgctc ccgctaatgt tgcgcctgcc gttacgctgc ttttaattat agctcccgtt
      661 tttaatgaat acatatattt caggtcttcg atggagctta ttttattttc cccatctatg
      721 tctattacct gccctcctat cattccttcc gtacctgagg agtcggcaat taatttcaga
      781 gcctttattg cattcatgcc cttgaaatta attgcagcat ctaaacctgt ttcaaaggct
      841 ttgttaagga gtgcatcacc ggcaagtatt gctcttgcct caccaaactt tttatgattt
      901 gataatttgc ctcttctata atcatcattg tccatggcag gcaaatcatc gtgaatcaat
      961 gaatatgtat gaatcatttc cattatgcag gcaaatggaa gggctttttt taaatcatcg
     1021 ctgaaaagct cataggctcc tagaaaaagc acgggtctga gtctcttccc tcccgacaga
     1081 aggctgtaat taactgcttg gtatattatt ttttgagggt tttcctttac ttcaataatt
     1141 tgccccagat attcatctac aatacttact ttttctttaa accactcttt aaattccata
     1201 ttaacatttc ctttgctctg aattctttta tgcaatctta tgcttcaccc gggatgccgc
     1261 aagctgtgac ctcggatgag ctgtcataca ggtgtagaat cttaggtttt tttaattata
     1321 tcataaataa gtaaattgta aaactattct gaaagcgcag ctttaaagat acaaaccgta
     1381 aatgaacgaa acatgggtaa agcctgcctt ctccaattaa tattaaattg actatggttt
     1441 gtacgtcgta tataatatat ctaggttatg gtaacgtatc cataaacttg taaatttttt
     1501 atatctgcac taggctgata taatagattc aattcaaaaa taatctagag gagcttttat
     1561 gaaaatgaga aaaatatttg cattgttttt agcattattg atggttgccc ttgtttttgc
     1621 aggctgtact cagacaagcc aaccttcggc gccgcaggct gacacacaac cgccgtctga
     1681 agaagagcaa caggctgatg aagcaaagag tgaacaaata ttaaggtatg gcgcatcagg
     1741 gtttaacggt atatttaatc ccattatgtt tgacaatgta tatgatggtt atatttcaaa
     1801 cataatattt gaaaagttaa taacgcacac tcctgaaggt gaatatatcc ccggaattgc
     1861 tgaatggaaa ttatcggagg ataaattaac ctacacattt acattaaaaa atggaatcaa
     1921 attttctgat ggcacggatt taactgcaga agacgtggct tttacatata tgactattgc
     1981 ccaccctgaa tacaacggac caatggcata tgcagtaagc aattttgaag gttatgacga
     2041 atatcacgag ggagtgacag atgaatttga aggtattaaa gttattgacg aaaaaaccat
     2101 atcatttact tttaaagatg gtagggctgc acctgcaaat attgaatgct tcgactatgg
     2161 tattatgtct tcagactatt atgcatttga aaattgggaa gatttcttag cattaattga
     2221 taaaccaatg ggctcaggca taatgacact ggacagctgg gaaccgaaac aatttatttt
     2281 attaaagaaa aatacaaatt attgggaccc tgaaaacggg gctaaaattg acagtctatt
     2341 ggtaagtgaa gttcctgagg aaagtatatt atcggcttta cagacaaatc aaatagattt
     2401 cgctcaaatt tcaccgagtg ccgaaaactt agaagcagca aaggctatgg ataatatcaa
     2461 catcgtaaac tatattggaa acggctatac gtttatgtgc tttaacacaa caagacctgc
     2521 cttagaggat gtacgagtca gacaagctct tatgcatgca cttgacagag aatcatttat
     2581 aaaggttcaa tatggtgaag gcttggcagc agtaggtatg gctcctatat ctccggcatc
     2641 atgggcattc cctgatttat cagagttgaa tccatattca ttcgatatgg agaaagctgc
     2701 agagcttatg gatgaagcgg gatggttaat ggaatcagac ggatacaggt ataaggatgg
     2761 gcagaaactt gctgttaatt ggcttgtttt cacagaggct gattggccga aaacattata
     2821 cgaaatggct gctgatacat ggaaacaatt aggtgttgac ctttctatgg aattaatgga
     2881 cggcaatagc gtcatagctc gtacaatagg tcccgagccg ggtgaaaaag actttgatat
     2941 ttacacattg gcctttacat tgacaataga cccggacccc acaggatttt tgtttgatga
     3001 tgatgcttat atggcaggcg gatttaacgc atcgggttat aagaatgccc aagcaatgga
     3061 ccttgtaaga gcaggaaaga aagaattcga catagaaaag cgtgccgaaa tttataaaga
     3121 atgggctaaa ataatgaatg aagaaattcc tcacgcaatc atagcataca gaagtgaaat
     3181 ttggggaata aacaaaagag tagatggaat gtttattgat acctatgcaa catgggactt
     3241 atctttgaag aacgttactt taaaataaca aaggggcagg cagtcctgcc ctaatttatt
     3301 gaccataggc tatatcatta taaaagaggc ggtattatga gatattatat tttgaggcgc
     3361 ataattcaaa taattcctgt actcatagga attatattta tattattttt tatcctcgaa
     3421 caagcaccgg gtactccggt ttcatatatg atgcatccga gaatgactcc tgagcaaaaa
     3481 gccgaactgg aggaaaaatt aggcttggac attccatggt atgaaagatt cgtcaactat
     3541 ataaaggagg catctcaagg taatttaggc tattcaagca cccacaagaa gcctgtaacc
     3601 gaagttatcg gaaattatgc gggacctaca ctgctgcttg ccttagtctc tttaacaatt
     3661 tctattttta tcggtattcc cgccggtata ataagtgctg taaaacaaca taccgtaata
     3721 gacaatatat taacggtgat gtcttttatc ggcataagca taccgtcgtt tttctttggc
     3781 ctcttgctgt taaaagcttt tgccgtagac atacagctct ttccgctgtt tgggctgaaa
     3841 gaccccttgt taatgtctga caatattttt gttaaaataa aagatattgc ctggcattta
     3901 gtattgccga gtattgtctt aggcttaaac tcaactgcaa gctttatgcg ttacacccgc
     3961 tctagcatgc ttgaagttat taagcaggat tatattagga ctgcaagagc caagggtctg
     4021 aaagaaaaaa ccattattta cagacatgca tttagaaacg gaataatccc ccttattaca
     4081 ttgcttggtt tctggatacc tttactttta tcgggagcga tagtaacgga aagtattttt
     4141 gcattgcccg gacttggaaa aatatctgtc gaagcagcaa tgtcaagaaa ctatcccctc
     4201 attctcggta tcaattcaat acttgcaatt cttacactga taggatcttt ggttgcagac
     4261 atactgtatg ctcttgcaga cccgagaata agatacgatt aggcaggtac atatggctaa
     4321 ggataaaaca aataataatt ataccggaaa tcaatcctat tggggacaag tactctcaag
     4381 actaagaaaa aacaaaatgg cgatgataag tctttacatt cttataataa taattgccct
     4441 atgcattatt attccaatta tatcacccta tgaaattcaa acaacgaatc tctcaggcaa
     4501 agatcaaaaa cctaattccc ggcacctttt aggcacggat ttaattggaa gagatatttt
     4561 taccagactg ttttatgcag gaagaatatc acttggcatt gcacttgcaa cggttataat
     4621 tgaatgtgta ataggtgttg ttttgggcag tttatccggc ttttacggag gaataattga
     4681 ttcgataata atgcgtatta ccgaaatgtt tatgtcattt ccttttatga taatatgcat
     4741 taccgttgta gcagtattcg ggaacagtat accgaatcta attttagtac tggctatatt
     4801 gagttggcca aacatagcac gtattgtaag aggacaaata cttgttctta gagaaatgga
     4861 atatatggag gcctgcgaag ctcttggcat cagcgaccta agaagaatat ttaaacacct
     4921 tttgcctaat gtatcagcct atgtaattgt ttatgcaaca cttgggatgg caagtgtaat
     4981 cttagtggag acttctttaa gctttttagg tctgggtgtt tctcctccta ctccaacctg
     5041 gggaaacatg attcaagaag caaggaatat actaataatt caacagaagt ggtggtactg
     5101 ggttcctccg ggaacagcta tattcatatc tgttatgtgc tttaacatat tgggcgacgg
     5161 catacgtgat gccttagacc ccaagatgat gaggtaattt tatggataaa aaattactgg
     5221 aagttaaaaa cctgaaaaca tatttttatt cagacggagt tattataaag tcagttgacg
     5281 atttaagctt ttacgttaat gaaggagaaa ctttgggtat agtaggcgaa tcgggatgcg
     5341 ggaaaagcgt agcctgtctg tctttggcac gcttggtgga aacaccgccg gggaaatatg
     5401 aatacggaga aatacttttt aagggtgaag atttgcttag ggtttcagat gcaagaataa
     5461 gacaaataag aggaaatgat atatctttta tttttcagga gcccatgact tcattaaatc
     5521 ctgtatttaa aatagggagg caaattagcg aggtcctgat tttacaccgg ggaatatcaa
     5581 aagaagaagc ttatgaagaa agtattcgaa tgctggagct tgtaaaaatt ccaaatcctg
     5641 aaagggtggt taatgactat ccctttgcat tatcgggagg aatgaggcaa agagtaatga
     5701 ttgcaatggc acttgcatgt aagcccggtc tcttgattgc tgatgagcct acaagcaacc
     5761 ttgatgttac aattcaggcg caggttttgg atttaatgaa tgagcttaaa gaaaaaatta
     5821 atacttcaat tatatttata actcacgacc ttgatgtaat tgcagaaatg agcgacagag
     5881 taatggttat gtacgcaggt aaggttgtag aagaagcttc aacaaacgaa atacttaaaa
     5941 acccgagaca cccgtataca gtcgggctta taaactcaaa accgaatatg gcaaaagaag
     6001 gtaccagact taatgtaata cccggtaatg tacctgattt aagcaatctt ccgccgggat
     6061 gttccttcca tccgagatgc gataaagcaa tggatatatg caaaagggtt attcccgatg
     6121 aaaaattatt atatgaagga cggcacagaa tttcatgctg gctttacgcc gattaggaag
     6181 aagtagatga gtgaatattt gattaccgta aataatttaa aaaagtattt tcccataaaa
     6241 aaaggacttg ttaagcgtac aagcggaaat gtaaaagccg tggacggagt atccttcaat
     6301 attcagaggg gacagattct tggaatagta ggagaatcgg gctgcggaaa atctactatg
     6361 ggaagaacca tcttaagatt gttgccaccg acaggaggtg aagtctttta tgaaggtaaa
     6421 aatgttttta atatgagtaa aagagaaatg aacaaaatgc gaaccaaaat gcagattata
     6481 tttcaagacc catattcttc attgaatcca aggcttcctg tttatgatat agtgggcgaa
     6541 gcaatgctac agcatggtat tgtaaaaaat aaaaaggaaa tgaaggaaaa ggtaattgaa
     6601 ataatcggta agtgcgggct ttccccggaa caggcagacc gttaccccca tcagttttcc
     6661 ggagggcaac gtcagcgtat ttgcattgca agggccttag ccgttaatcc tgattttatg
     6721 gtctgcgacg aatcagtttc tgcattggat gtttctatac agtcacaaat aataaatctg
     6781 ttaaaagatg cacaggaaga ttacgggctt acctatatgt ttatatctca tgacctgtcg
     6841 atagtaaaat ttatcagtga tgagatagca gttatgtacc ttggtcaaat aattgagaag
     6901 ggcagtaaaa aacaaatatt ttcaaatccc ctccacccgt acacggaagc attattgtca
     6961 gctgtccctt cacttaaaca aaacaaaaga agaatagttt tgaatgggga tataccatcg
     7021 ctggtaaatc cgccaaaagg atgtcgtttc tatacacgat gtccctatgt aaaagatata
     7081 tgcaaggaag aaatacctga atacagggaa ataaataccg gacattttgc aatgtgtcat
     7141 aaggtaaatg gagtatttta atgtttttaa aaaagaatag agtagatgaa gatttggaaa
     7201 gaattcggga agctaacctt cccaacaaag aagaaataaa aatgaacgcg aagcattcag
     7261 atgaccttag tctggaaaaa ggtgatcttc ttgcgatggt attggcagta atatctctga
     7321 tactgcccta tgtaattgct tttttggggg ctatcggagt tgtgatattt tttatgtatt
     7381 tgttttttag ttgaaaaata tacgacaata agctatagca gaggaatatg gaagtatata
     7441 aaaaatcggg atttctcccg attttttctt ttacagctgc ttatattctt tgaacgttag
     7501 ccgcttggtc tcctctgttt ccttgagtaa catcaaagct tactctttga ccttcttcaa
     7561 gagttttgaa tccgtctccc tgaatagctg aaaaatgtac gaaaacgtca tttccatctt
     7621 cttttgtgat aaatccaaat cctttttctg agttaaacca ttttactgta ccctgtgcca
     7681 tgagtacctc ctaaaaaatt attatattcc atagtaaaaa aatttcacac atttttacac
     7741 actatatacg gcacgccaca tataatatct aaaaacatgt gaaatccata aatacatctg
     7801 taatacattt taagactaac acatcaagct ttatatgtca acaattattt ttttggaaaa
     7861 tcaaggaaca attggatatt cgttttggga cggttctttt atcggtgcct ggcaccacaa
     7921 atggtgcccg gcaccgatac ccaaaatttt attttttatt caagaagctt ggtattgtaa
     7981 aatcgaacgg gttttccggt ttcttttcgc ttttctcact cggttcccca tcggattttt
     8041 ttgctccgtc tgcaaaacct gtcgcaataa cagtaatttt aatagtatct ccggcagatt
     8101 cgtcaatatt tgctccatat attaagtttg cttccgggtc agcagcttct tcgattaatt
     8161 ttgctgcttc gttaacttca tggattccca tgcttggacc acccgtaata tttaccaaca
     8221 cacctcttgc tccattgatg gatgtttcta aaagaggact ttgtattgcc tgtttaactg
     8281 catttaatgc tctgttttct cctgaagcca ttccgattcc catgtgagca ataccttgac
     8341 cggacataat tgtttcaacg tcggcaaaat caaggttcat tatggcaggg attgctatca
     8401 aatcagatat accctgtata ccttgtctca atacttcatc tgccatggca aaagcttcca
     8461 gcatggttgt tttcttgtct gttatctcca aaagtttgtc gttcggaata acaaccaaag
     8521 tatctatatg atcctttaac cttttaagtc ccatttcagc atttctcatt ctgcatcttc
     8581 cttcaaacat aaaaggttta gttacaactg caaccgtaag tatacccata gatttggcaa
     8641 tattagcaat aataggagct gcaccggttc cggttcctcc gcccataccg gcggttatga
     8701 aaaccatatc tgcgccttct accagttctg ctatatcttg tttattttct tcagctgctt
     8761 tttctcctat ttccggtttt gagccggctc ctaatccatt agttaatttt tcacctattt
     8821 gaaattttgt ttctgctttt gataagccaa gagcctgctt gtccgtattt atgctgataa
     8881 aattcacacc tctgacaccg gcatctatca ttctgtttac tgcattattg ccgcccccgc
     8941 ctacacctat tactttaata ttggcaagtc tttctgatgg ttcctcaaat tgaaacatat
     9001 tttgacctcc tgagagagaa atttttcata ctatttaaac tattctatca taaatttaat
     9061 aatattcata catatttttt ataatctaca ccaattttcc gaatattttt tcagaattat
     9121 cacaatattg agtttacttg tttataaaat acttttcaag atatatatcg actatgactt
     9181 ttatcttttt ataaatatta ttaccgaatg caaatactgc agctaaataa ataggcaaac
     9241 ctaattgctc gcccacaaat cccaggaaca aagcaaataa caagtctgtt aacaagaatg
     9301 caatactttt tgtaattgtc aattctgatg ttttattttc atgaagtata tttataattg
     9361 tatttaatat tgccagtatt gccagtgata tatacactat ataatccgga ttgtaaataa
     9421 aatttaagtt caatcctgca actataccta agattaatcc gattgcaaca tatatcattt
     9481 cttttcccct tcttctacta tttcggcata tttggtttca taatttattc cgcgaaattc
     9541 tctgaccgtt aaattatatc cttgtgcaac aactacttcc attccgtacg ttttcttcaa
     9601 ctcgtaagca taagttcctt cttcggtaac tgcatcatac aattcatcca tatctccgat
     9661 tgctattatg acaaaaggag caggtattac ttcaccgttt actctgatta aaggtcccgc
     9721 acatactact tcagatatgt taattattct tttcccgtta acttcaattg cttccgctcc
     9781 tgctgccttt aaatcgttta acagtacaac aatatctaca tcatggacta ttctttccat
     9841 taactcaagg tcgtagtctt cattgatgtt gtctgaaact ctcagcatta tacccggtcc
     9901 tcgcacagcc gtaaacccat tatatagttt aatctgctca atttgagatt taatttcaat
     9961 tatgtcgtct aaatattttt ttgattcata gtcggcaatt gttctttttt cgctttctac
    10021 agcattgcgc atattattta ccaagagctt catttctttg atactgtgtg catatttgtt
    10081 caaatctctg attgtatata tggtatactc atctattcct gcatctgcac cgtttacaat
    10141 tgctgcagct gtcaaaaaaa ccagcacgaa aattattata tttaatgttt ttttaatagt
    10201 gctcactcct tagatttctt catcccgaat tattggaagc gcatacttga actgtttttc
    10261 tccgtcataa cgcggaataa aaatttcatc cgaatcttcc gtttcaatta tatatcctat
    10321 ttcattaagt tgagaaacta tacctcctac aaagttaaga gaagaattga ttgtagatct
    10381 gtctccgatt gcctttattt ccaaaggaag taccatggcg acaccgttta cgttaatatg
    10441 ttcaagaacc ggcactattt ccgtatagtt ggtatatctt tgtccattga tggaaatagc
    10501 ttcaacaccg gcattgttta aatagctgat taaagctaat atatactgat gactggatgc
    10561 aagacttaaa tatgtatccg gctcggcatc tatggttatt atggttccgg gtcctttaac
    10621 atcataatag cctgatatta tcttctgaac tttaagttct tctttaagct ttaatagttc
    10681 ttcttcagca tccttgaata attcttcact ttctctgctt tcttttttaa gtgattcaag
    10741 ttcatcccta agtctgtttt tttcgatgct tagcttagcc agttcctcat tcagctcaac
    10801 tgctttttta tctaaattaa cggtgcccaa tatattgctc tttgttctca tttgagtaac
    10861 caagataatc cctaatatta agcttataga aaacatcatt actttttgcc agattttgtt
    10921 cttcatattt cctccgttag tcctcaatat atacaggact gtcacctttt gtaaaatcaa
    10981 ttaaccccgt agctaccgtt aaattattgt ttaatctttc gttaattatt ttcattccaa
    11041 aaacaatctg ataattaatg tcctcgctta agtctatcct tactgcagtg ccatattgtg
    11101 ttttaaagag gaagctgttg ttcaattcga tattaaaggc ttttattata tcgcttccat
    11161 attcattgtt taaatactct attgcattga atactttttc attattttca acgcctgcaa
    11221 attgtatatt atcccctata ttgtatacta catccgtata actttcaata gttaataagt
    11281 ctaatttctc attacaggtt ctcaatacta ttccattttt atcgattatt atgtattcgt
    11341 tgttgaagaa aacctgatat gtatccaccc tttcttttat atccaggaat attttattcg
    11401 gaagttcata aaccaccctt gcattttcca cgtatacttc attttcaata ttctctttaa
    11461 cctttgacct gtcaacttca aatatttttt taccgacttc aatacctgat ttttccatta
    11521 tatattcatc actatatgtt aaattaccat caatgtcaac acctataagg ttgaaatatg
    11581 gagttttata atatacatag cagatacaaa aaacaacaac tacagcagcc gtaataaaag
    11641 ctgctgtgct gaccataagc tttttatttc gtcttctttt ctttttcctc tgtgtttttt
    11701 tgttgttgtt ttcatccatt gtttgttttt tctcccgatg tgtcattcag tcatcgctat
    11761 gaatactcgt ttattaattg tcattctgag agctagctcg aagaatctca tgagatcctt
    11821 cgcttacgct caggatgaca tacttcagaa atccttcgct tacgctcagg atgacatatt
    11881 tcagatatcc ttcgcttact attaagatga caatttttaa atttatctta cactattcaa
    11941 tttcggtaat tattactcct aagttttgta atttatatat aaatttttca tatcctcttt
    12001 ttatatgatg gatattatta atcttggatg tgccttctgc caccatagct gcaattacaa
    12061 gtgcagcacc tcctcttaaa tctttggctt caacttcggc gctgtaaagt ttttcaactc
    12121 cttccacaac tgctactctc ccgatagttg taatatttgc tcccattttt tgcagctcgg
    12181 gaacatgttt tagcctgctg tcaaatactg tttcttctat aattgaagtt ccgtcagcta
    12241 cggtcatgga tgccataacc tgtgcctgca tatcggtagg aaaacccgga tagggttgag
    12301 ttgttatttt ttctattgct tttatccttt tgttatttgc aataactatt gttccgtgtg
    12361 tttctcttat ttcgcatccc gcatcttcat ataaagaagt aatagactta aaatgagatt
    12421 tttctatgtt tcttatgtaa attttgctct tcatcgatgc tgctgcactc aaataagttc
    12481 ctgcctcaat acggtcggaa ataattttgt attctacagt atctgtgttt tgaacttttt
    12541 ttccttctat agtaataata cctgttcctg caccacttac acaaaaacca caagtattca
    12601 aaaaattttg caagtctgta atttcaggct ccctggcagg attgtaaata gtagtctttc
    12661 cggggtttcc gacggcaaaa agcataatat tttcagttgc cccgacactc ggatagtcaa
    12721 gatgaatatc acaactaagc gcctcattta ccgagccgac cagcaaacca tttttttcag
    12781 ttatttcaac tccaagcttt cttaatccct ttaaatgcaa atcaattggt ctaagaccta
    12841 tttcacaacc tccgggatat gcaacttcca ctttccccgt gcgctgcaaa acagccccca
    12901 ttaaaattat tgaggacctt attttcctta ctaatttttc aggtactata tgagtattca
    12961 aacctcttgt atcaattaac gcaataccat tttcaaattg gacactgcat cccatattac
    13021 ttaatatttc aagcatgtct tttacatctt caatatcagg tatattttca agaatatatt
    13081 ctctaccggt gattacagtg cttgccagaa taggaagagc agcatttttc gagcctccga
    13141 tatcgacaat accttcaaga ctgtttcttc cttcaacaat aaagcttccc atttttatcc
    13201 ctccgcaaca agttgattca tgttatatgt tatgcat
//
LOCUS       DAOSHP010000051        13087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_3988_length_13087_cov_9.594153, whole genome shotgun sequence.
ACCESSION   DAOSHP010000051 DAOSHP010000000
VERSION     DAOSHP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_13087_cov_9.594153"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PLB33_06155"
     CDS             complement(<1..831)
                     /locus_tag="PLB33_06155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOG63141.1"
                     /translation="MKKRLLGNTGMEVSVIGFGGIPIQHVSEKEAINLILECKQQGIN
                     FIDTARVYTVSEKLIGKGLREAGRENFYIASKAMNYTYDGMKSSIEDSLNDLEIDCID
                     LYQVHNVSSKKDLDTVFSENGALKALVEAKEQGLIKHIGITGHIRELLMEAIDNYEFE
                     TLQFPFNPVEPQGTELLLKALKKGMGTIAMKPMAGGTFSYPLLSLKYIMNSGLITVAI
                     PGMDTIEQVIENARAGIDDEPLTKEEQEIINKEVEILGTEFCRRCGYCKPCPEGIDIP
                     S"
     assembly_gap    1233..1332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1333..1992
                     /locus_tag="PLB33_06160"
     CDS             <1333..1992
                     /locus_tag="PLB33_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG63142.1"
                     /translation="LTAIINNALTKVINIFMAFANTLLSIIISVYILIDKESIINWFK
                     EFASLFIGRKAANDIVRISHILYRNVSSYISGKATASLIIAFLIYIGSKYIIKCPYPA
                     IDGMVIGITNMIPYFGTFIGSVPIVLINVLYTPQKGALLFILILILQQLESLIIDPKI
                     LGSQLSIKPLLVIISIIIGGGLFGPLGLFFAVPVAALIKSIVDVYMLKSLKNVHIIDK
                     S"
     gene            2223..4547
                     /locus_tag="PLB33_06165"
     CDS             2223..4547
                     /locus_tag="PLB33_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG63143.1"
                     /translation="MKKTLLVIIILIFVASFINIYAAVFPDIKKDPYLEEAIGVLSSY
                     KVVQGYPDNSFKPDRTVTRAEMAKVVTVAAGFNEYSKNMTSSYEDMHGHWAESYVELA
                     DVLGIVKGISPSVYGPDNLIKFEEAYTMILRLLGYTDESLMGSWPSNYREKAIELNLF
                     ENINSNTEFASRRDITIMLFNALSKNLVSVKENNTYNVTNKTLISKLGKMETRVVSIK
                     DLNIDNFDYTDYLFNKWDIYYDNYGNTVYVSNPQYNEFSGTVTSILSNRVIFVTDDYG
                     NVRVFQVPDIPIIINGERGSFNNLNESSVKIIYEEDSFKGEVIGIIAYKATDVIVVDR
                     DDLYKEGSRQFAGKNLPLSNSEVNFNKINIKGDAKSLEDIKVNDVVYFYETQNDKNSS
                     LTLNVLRSKVEGVVTSVDNVNGATFYTINNISYMTGKDYIFTEKANVNDSVTLILDKN
                     NNIIKLYINKYGKRPSTYGIVINSSSGTNGNAEARILDEYGRIRTYSLADNSSVVRVH
                     ENDSSVLKQSLIGSNDLVMFDPVSNGTLKIINLMQTRNIASPYNNQTQTLENGGYKIS
                     SDTFIVYRNNGQYQLLVPSQLGEYLEGRAVVNYMGHIDAIYLTKGIKESNVITITPDT
                     PQNYNGTIYGIIKGVTKLDDTTSHIQFFNDSNVFSVSNTSVAGKSISSMMNSYVRAVI
                     VNGMVSSIEKVTPETSRIKISQIYTNQMLIDNITYMEYAQDVKVYVCTLDSSGNISDF
                     KEGTKSDIKAGSTAQLYNLYGGFDGVIDVVFVFK"
     gene            4751..5881
                     /gene="alr"
                     /locus_tag="PLB33_06170"
     CDS             4751..5881
                     /gene="alr"
                     /locus_tag="PLB33_06170"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOG63144.1"
                     /translation="MNEKLRPAWVEINREKAIHNFLEVRRAVGPDRKICAVVKADSYG
                     MGALELSKMYLENGVDMFAVAVIGEAFELRKEIKDKDILVLGFTSEEFYDDAIENNIT
                     LTIYDYNHALRLNETAKKLNKQAKIHIKVETGMNRLGFLPTAENADKIADIAKFENIK
                     IEGAFSHQAKADEKDKTTAHMQADRFIKFIGMLEERNVQIPIKHIANSATIIELPEYY
                     FDMVRPGIILSGFYPSDEVDTEKYKFKICVTLKAKVANVKTIEAGEGVGYGHLYRAEK
                     PVVVGTIPLGYADGYSRLLSNKGYVVVKGVKCPILGKVCMDQFMVDLSKVENPQIGDV
                     AIVYGDGTDGAMTAEDVANMRGTISYEVLTNLAVRRLPKIYV"
     gene            5892..7121
                     /gene="glyA"
                     /locus_tag="PLB33_06175"
     CDS             5892..7121
                     /gene="glyA"
                     /locus_tag="PLB33_06175"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOG63145.1"
                     /translation="MNFNLIEENDNELYRFIMEEKERQRINIELIASENFVSDTVLQA
                     MGSHLTNKYAEGYPGKRYYGGCEFVDKVENLAIERAKKIFGADHVNVQPHSGSNANFG
                     VYFAVLKPGDKILGMDLSHGGHLTHGSPVNLSGSYFNVVFYGVNRETETIDYDQVREI
                     AKKERPKLIVAGASAYPRIIDFKAFRDIADEIDSYFMVDMAHIAGLVAAGLHPNPVDY
                     ADFVTTTTHKTLRGPRGGMILCRQEFAQKIDKAIFPGTQGGPLMHIIAAKAACFKEVL
                     DDEFVLYQQQVIKNAKKLSEELMNKGFRLVSGGTDNHLMLVDVRNKNLTGKEAEKILD
                     EIHITTNKNTIPFDTQSPFITSGIRIGTPAVTTRGMKEAEMIELADIMDSALSKKENE
                     SMLKERVLKLTSKFSLE"
     gene            7327..8538
                     /locus_tag="PLB33_06180"
     CDS             7327..8538
                     /locus_tag="PLB33_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63146.1"
                     /translation="MKRILSLLLSVLMVLALFTACTPASNNGNGNEEPETGNEEEPVA
                     EDADVIKIGVFEPLTGMNGGGGLQELGGIEYANKMYPEVLGKKIQLVVVDNKSDKGEA
                     TTAVTRLIEKDKVVAIIGSYGSAVSIAAGDIIKNAQIPAMGASCTNPMVTQGNEFYFR
                     ACFLDPFQGKVMATYAIEQGAKTAAVIMQNGDDYSVGLANFFINAFKDLTGDENSIVD
                     VSEFQLNESDFNAILTNIKAKNPDVIFAPSAATTAPLIIKQARDLGIDSLIMGGDTWE
                     NPAIIDVAGEDAEGIVLSTFFDENDPATEEARKFVEGYKKELAGKEPIIPAVAALGYD
                     AYILLRDAIERAGSTDGVAIRDALKATKDFEGVTGIINFTEEGDADKNLAVIKTVKDG
                     QFVFVDSVEVK"
     gene            8611..9504
                     /locus_tag="PLB33_06185"
     CDS             8611..9504
                     /locus_tag="PLB33_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG63147.1"
                     /translation="MTGEIFMQQLANGISLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMMAAYFMVFSIITFNIPWFISIIITIIATVTLGVLVEKTAYRPLRNAPRMSIMISAI
                     GVSFLLENLATYLFTGVPRGFPDIKVLTTTMKLGSLSLTVATLITPFVTLMLLYVVLF
                     ITNKTKIGMAMRAVSKDFETAKLMGININQVISITFIIGSFLAAIGAVLWGSRYPSVM
                     PLMGVMPGLKCFIAAVLGGIGNTTGAVLGGFILGMAEIMLITFLPSLTGYRDAMAFVI
                     LIIVLLVKPTGLLGEKVTDKV"
     gene            9501..10595
                     /locus_tag="PLB33_06190"
     CDS             9501..10595
                     /locus_tag="PLB33_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG63148.1"
                     /translation="MMDKLNNKNNKLILNILLIIIFASIIILLDTKKIGDAYIRRILN
                     LSAIYAIVSVSMNLVNGFTGLFSLGQAGFMAIGAYTVAIFTVPLAARAKIFYITPQNP
                     VLAQIELPFVIALILGGLLAALVALLIGLPVLRLKSDYLAIATLGFSEIIRIVFTNIK
                     SITNGALGIKNIPTMPTMWVFFGIAIISIGFMWLLINSSYGRAFKAIREDEVAAEAMG
                     INLFKHKVMSFSIGAFFAGIGGGLLATLFATVDPNQFYFPVTYNFLLIIVLGGMGSIS
                     GTIIASFVVTSGLEILRFFDEPLSLFGLNIPLFRPGLRMVIFSILLMVLVLFFRNGLL
                     GQKELSWKLIFDLLSGKKPRKKTGEGGAAQ"
     gene            10592..11362
                     /locus_tag="PLB33_06195"
     CDS             10592..11362
                     /locus_tag="PLB33_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63149.1"
                     /translation="MSVLKINNVTMQFGGVTAVNNLNIEVQEGRIEALIGPNGAGKTT
                     AFNVITGVYSPTKGNVFYKNENITGLTPDKITKKGIARTFQNIRLFKNLTVLENILIA
                     NHLRINSNFLSSTLRMPWSNKEERQMREKSEMLLDKLDLLRLKNEIAHSLPYGEQRKL
                     EIARALATDADLLLLDEPAAGMNPSETMELSSFIHNIRDEFHLTIFMIEHHMDLVMEI
                     SEKIYVLDFGQLIAEGTANEVKNDQRVIEAYLGVEEDA"
     gene            11355..12062
                     /locus_tag="PLB33_06200"
     CDS             11355..12062
                     /locus_tag="PLB33_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63150.1"
                     /translation="MLKINGLHVNYGGISAVKGISIHVPEKSIVTLIGANGAGKSTTL
                     KAIAGLVKARNGEIIFQNENILGKDTIDIVARGITMVPEGRKVFVNLSVLENLRVGAY
                     LRRDGIEDDIKRVYDLFPILKERAWQLAGTLSGGEQQMLAVGRALMARPKLIMMDEPS
                     LGLAPLIVNSIFEIIKEINKQGTTILLIEQNANKALKVADYGYVLETGNITMEGKGIE
                     LLKNEKIIEAYLGTSKK"
     gene            complement(12147..13037)
                     /locus_tag="PLB33_06205"
     CDS             complement(12147..13037)
                     /locus_tag="PLB33_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOG63151.1"
                     /translation="MWEVIEHALEHTFKDSFKMLPFLFAAYIIIEFIEHKSSDKLVSG
                     LQKFGVVGGAVLGGVPQCGFSVAASNLYAGRIITAGTLIAVFISTSDEAIPILLSSNG
                     NLNVILLLITVKAIFAVIAGAMADFIFKGFFNKSNNEGIASSIHHHVCTDCGCHDDPG
                     ILKPALKHTINIFIFLFITNFLINFFIEYVGEDNLSEILLHDSIFQPALAGIIGFIPN
                     CAASIILTQLFIAGSISFGSVIAGLSTGAGVGLLILFKVNRNINENLKIMLYIYVFSV
                     LAGSLIQTMGTYLTVPHGFF"
BASE COUNT         4437 a         2055 c         2537 g         3958 t
ORIGIN      
        1 gctgggtatg tctattcctt cgggacaagg cttacaataa ccgcatcgcc tgcaaaattc
       61 agttcctaat atttcaactt ccttgtttat tatctcttgt tcttcctttg ttaaaggctc
      121 gtcatctatt cctgcccttg cattttcaat aacctgttct attgtgtcca taccgggtat
      181 tgctactgtt ataagacctg aattcataat atatttcaaa gataacaatg gataactgaa
      241 tgtaccgcct gccatcggct tcattgcaat tgttcccatc ccttttttca atgcttttaa
      301 caatagctcc gttccctgcg gctctacggg attaaaaggg aattgtaaag tttcaaattc
      361 ataattatca attgcttcca taagaagttc tcttatatga cctgttattc ctatgtgttt
      421 tattaatcct tgctctttcg cttctactaa tgctttcagt gctccattct cggaaaaaac
      481 ggtatctaaa tcctttttag agcttacgtt gtgaacctga tataaatcta tacagtctat
      541 ttccaagtca tttaaactgt cttcaattga gcttttcatt ccatcgtagg tatagttcat
      601 tgccttagag gcaatataaa aattctcacg acctgcttcc ctaagacctt tacctattaa
      661 tttttcactg actgtataaa ctcttgccgt atcaataaaa ttaatccctt gttgcttgca
      721 ttcaagtatt aagttaattg cttctttttc actgacgtgt tgtatcggaa ttcctccaaa
      781 ccctattaca gacacttcca tacccgtatt tcccaacaat ctttttttca tatgccacct
      841 cattgttttt atgggttaat tatatattag aaatttattt gagtcaatgg agaatagaca
      901 aggggagtta tagggcatac aaaaaggaca tcccgcaatg tttgtttcgc gggatgttta
      961 tataatttgg aagaagataa aaaaagtatt atcaacataa ttatttacag aaattcatta
     1021 tttatacata atttttgaaa aattttaata tgaataataa gacgcaataa tagggggtaa
     1081 ggtacacttc tttctgtgta ggtgataatt tgaaaaagaa tagtcttatt attttacctg
     1141 tttttatatt ttatattgca gttttcagat ttatttattt agagaacggt ctgtctaaga
     1201 taatccaagt atgctttccc atattttcag ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnctgacagc aataataaat aatgctctca ctaaagtcat caacatattc
     1381 atggcatttg caaatacatt gttgtcaata attatttctg tatatatttt aattgataaa
     1441 gaaagtatta taaattggtt taaagaattt gccagcctgt ttataggcag aaaagcagca
     1501 aatgatattg ttaggataag ccacatcctg tatcgcaatg tttcaagcta catatcggga
     1561 aaagcaactg cttctctcat tattgcattt ttaatttata taggctcaaa atatattata
     1621 aaatgtcctt atcctgcaat tgatggaatg gttataggaa ttaccaatat gattccttat
     1681 tttggcacat ttatagggag tgttccgatt gtgttgataa atgttttata tactccgcaa
     1741 aaaggggctc ttttgtttat tttaatccta atattacagc aactggagag tcttataata
     1801 gaccctaaaa ttttaggcag tcagttgtca ataaagccct tattagtaat tatttcgata
     1861 ataatcggag gaggattgtt tggtccctta ggtctttttt ttgcagttcc tgttgcagca
     1921 ttgattaaat caatagttga tgtctatatg ttgaaatccc taaaaaatgt tcacattatt
     1981 gataaatctt gatataattt gtgagtttgt ggataaaact gttgacaagt cgcccaaaag
     2041 ccataaaaca tgtgtataag tattatgaaa taaagttgaa aaacagtctg gttttaaagc
     2101 agaaaaaagc attgtaatgt taatgtaaca ctaattaaat aaatataatg tataataata
     2161 ttaaagaaat actattacta tgcatagtat ttttttaatt gaatgcttta aggattgatt
     2221 atatgaaaaa aacactattg gtgataataa ttttaatatt tgttgcatct tttataaata
     2281 tatatgctgc tgtctttcct gatataaaaa aagatcctta tttagaagaa gcaataggcg
     2341 tattgtcaag ttataaggtt gtacaaggct atccggataa cagctttaag cctgatagaa
     2401 cagttactcg tgcagaaatg gcaaaggttg taactgtagc ggcaggattt aatgaatact
     2461 ccaaaaatat gacttccagt tatgaagata tgcatgggca ttgggctgaa agttatgttg
     2521 agctggctga tgtattaggt attgttaaag gaatttctcc atcagtctac ggacctgata
     2581 acctgataaa gtttgaagaa gcctatacaa tgattttaag gctattagga tataccgatg
     2641 aatcactgat gggaagctgg ccttctaatt atcgtgaaaa agcaatagaa ctgaatttat
     2701 ttgaaaatat taattcaaat accgaatttg catctcgaag agatattaca ataatgttat
     2761 ttaatgcact tagcaaaaac ttagtttcgg taaaggaaaa caatacttac aacgttacaa
     2821 acaaaacact tatttcaaag ttaggcaaaa tggaaacaag agtagtatca atcaaagact
     2881 taaatattga taattttgac tatacggatt atttgttcaa taagtgggat atttattatg
     2941 ataactatgg aaatacagtc tatgtaagca acccccaata taatgaattt tcgggaactg
     3001 taacaagcat actttcaaac agagtaatct ttgtaactga tgattacggc aatgtgagag
     3061 tttttcaggt tcctgatatt cccattataa ttaacggaga aagaggaagc tttaataatt
     3121 taaatgaatc cagcgtaaaa atcatctatg aagaagattc tttcaaagga gaagttatag
     3181 gtatcatagc ttataaagca actgatgtta tagttgtcga cagggatgac ttgtataagg
     3241 aaggaagcag acagtttgca ggtaaaaacc tgcccttgag caattcggaa gttaacttta
     3301 ataaaattaa tattaaaggc gacgcaaaat ctttggagga tataaaagta aacgacgtgg
     3361 tgtactttta tgaaacacaa aatgataaaa actcctcatt gacacttaat gttttaaggt
     3421 ctaaggttga aggcgttgtt acaagtgtag acaatgtaaa tggtgctaca ttttatacca
     3481 taaacaatat aagctatatg acaggaaaag attatatttt tacagagaag gcaaatgtaa
     3541 atgattcagt tacattaata ttggataaaa acaataatat tataaagctg tatataaata
     3601 aatacggcaa aaggccatcg acttacggca ttgtaatcaa ctcgtcaagc ggcacaaacg
     3661 gtaatgccga agcgcgaata cttgatgaat acggaaggat tagaacatat tccttagcgg
     3721 acaactcaag tgttgtaaga gtgcatgaaa atgattcaag tgttttaaag caatcattaa
     3781 taggaagcaa tgatttagtt atgtttgacc ctgtgtcaaa cgggacatta aaaataataa
     3841 atttaatgca aaccagaaat attgcaagtc catacaataa tcaaacgcaa accttagaaa
     3901 acggcggtta taaaatatca tcagatacat ttattgttta cagaaacaac ggtcaatacc
     3961 aattgttagt accttcccag ctgggagaat acctggaagg aagagcagtt gttaattata
     4021 tgggacatat tgatgccatt tatcttacta agggaataaa agaatcaaat gtaataacaa
     4081 taacaccgga tacaccacaa aactacaacg gaaccatata cggcataata aagggtgtta
     4141 caaaattaga tgatactaca agccatatac agttttttaa cgacagcaac gtattttctg
     4201 tttcaaatac atctgttgcg ggcaaaagta tttcatcaat gatgaactcc tatgtaagag
     4261 cagtaattgt taacggtatg gtttcaagca ttgagaaagt aactcctgaa acatcaagaa
     4321 taaaaatatc tcaaatttat accaatcaaa tgttgattga caatataacg tatatggaat
     4381 atgcacaaga tgtaaaagtc tatgtttgta ccttggacag ttcgggaaat ataagcgatt
     4441 ttaaagaagg tactaaatca gatattaaag ccggctcaac agcccagctt tataacctgt
     4501 acggcggatt tgacggagtg attgacgttg tatttgtgtt taaataataa gtttcttcag
     4561 gctgcgcctt cagaatgaca agattgtata ctttgtcatc aaactgaggg cgtatgcttt
     4621 ttttgttgat taattgactc tggtttgcta taattttatt aataatacaa gttggggaat
     4681 attcctcaaa gcttacctgc aaaggttatc atagaggtgt tctccatacc actaaaggag
     4741 gagcaattaa atgaacgaaa agctgaggcc tgcctgggtc gaaataaaca gagaaaaagc
     4801 cattcataac tttttggagg taagaagagc agtaggacct gacaggaaaa tatgtgccgt
     4861 tgtaaaagca gacagctatg gaatgggagc tttggaatta tcaaaaatgt atcttgaaaa
     4921 tggcgtagat atgtttgccg tggcagtaat cggagaagct tttgaactga gaaaagaaat
     4981 aaaagataaa gacattctgg tactaggttt tacatcagag gaattttacg atgatgcaat
     5041 agaaaataat attacattga ctatttatga ctataatcat gcattaagat taaatgaaac
     5101 agcaaaaaaa ttaaataaac aagcaaaaat tcacataaaa gtagaaacag gcatgaacag
     5161 attaggtttt ttgcctacgg cagagaatgc ggataagata gctgatattg ctaaatttga
     5221 aaatattaaa attgaaggtg cattttcaca tcaagctaag gccgatgaaa aggacaaaac
     5281 aaccgctcac atgcaggctg acagattcat taaatttata ggtatgctgg aagaaagaaa
     5341 tgtgcaaata cctataaaac atattgcaaa cagtgctact ataatagaat tgcctgagta
     5401 ttattttgat atggtaagac cgggcattat tttatcagga ttttatcctt ctgacgaagt
     5461 ggataccgaa aaatataaat ttaaaatttg cgttacattg aaagcaaagg ttgcaaacgt
     5521 aaaaacaatt gaagccggcg aaggtgtcgg ttatggacat ttgtaccgtg cagagaagcc
     5581 ggtggtagta ggcacaattc cgttaggata tgctgacgga tattcaagat tgctgtcaaa
     5641 caagggatat gtggttgtaa aaggagtaaa atgccctatt ttgggaaaag tatgtatgga
     5701 ccaatttatg gtggatttgt ctaaggtgga aaacccacag ataggagatg tggctatagt
     5761 atacggtgac ggtactgatg gagcaatgac agcggaggat gtagccaaca tgagaggtac
     5821 tatttcttat gaagtactga caaatttagc cgtaagaaga cttccgaaaa tctacgttta
     5881 aggagtacta tatgaacttt aatttgattg aagaaaatga taatgagttg tatcgtttta
     5941 taatggaaga aaaggaaaga caaagaatta acattgaatt aatagcatcg gaaaattttg
     6001 tttctgatac ggttttgcag gcaatgggaa gtcatttaac caataaatat gctgaagggt
     6061 atccgggcaa aaggtattac ggaggatgcg aatttgttga taaggtagaa aaccttgcaa
     6121 tagaaagggc taagaaaata tttggtgcag accatgtaaa tgttcaacct cattcaggct
     6181 caaatgcaaa ctttggagta tactttgcag tactgaagcc gggagataaa atattaggta
     6241 tggacctatc tcacggaggt catctcactc acggaagtcc cgtaaattta tccggctcat
     6301 acttcaatgt agtgttttat ggagtaaaca gggaaactga aaccattgat tatgatcagg
     6361 taagagaaat agcaaaaaaa gaaaggccta aattaatagt ggcaggagca agtgcttatc
     6421 cgagaattat tgactttaaa gcattcaggg atatagcaga tgaaatcgac tcttacttta
     6481 tggttgatat ggctcatata gcaggacttg tagctgcagg actgcatcct aatcccgttg
     6541 actatgcaga ctttgttaca acaaccactc acaaaacctt gagaggtcca agaggcggaa
     6601 tgattttgtg caggcaagaa ttcgcacaaa aaattgataa ggcaatattt ccgggaacac
     6661 agggaggtcc tttaatgcac attattgccg ctaaagccgc atgttttaaa gaagtcttgg
     6721 atgatgaatt cgtattgtat cagcagcaag ttataaaaaa tgcgaaaaag ttatccgaag
     6781 aacttatgaa taagggattt agattagtat ccggcggtac tgacaatcat ctgatgttgg
     6841 ttgatgtcag gaacaagaac ttaacaggca aagaggcaga aaagatactt gatgaaattc
     6901 atattactac aaataaaaat actattccat ttgatactca aagccccttt attacaagcg
     6961 gaataagaat aggaactccc gcagttacca caaggggtat gaaagaagct gaaatgattg
     7021 aattagcaga tattatggat tcagcactat ctaagaaaga aaatgaaagt atgttgaaag
     7081 aacgcgtatt aaaactgact tcaaaattct ctttagaata aaacagacaa cccattaata
     7141 taatagtatc agtaatgaca atctgactgt tttttcatat agcagcatag tttttatttt
     7201 acatacaaat tatgctgttt gtatgtaaaa tgtgcaagac tgtttacata gcgcggaaaa
     7261 ttttcttgca ataatatttt ttaaatgtta taatcataga aatttataat aatgaggaga
     7321 gagtatatga aaagaatttt atctttgtta ttaagtgtgt tgatggtgct tgccttgttt
     7381 acagcttgta ccccggctag caataacggt aatggaaatg aagagccgga aacaggaaat
     7441 gaggaagaac cggttgcaga ggatgcagat gttattaaaa tcggtgtgtt tgaaccgctt
     7501 acaggtatga atggcggcgg tggtcttcag gaacttggag gcatcgaata tgcaaacaaa
     7561 atgtatcctg aagtattagg caaaaaaatt caattagttg tagttgacaa caaaagtgac
     7621 aaaggcgaag caactaccgc agtaacacgt cttattgaaa aggacaaggt tgttgcaata
     7681 attggttctt acggctcagc tgtttctatt gcagccggtg atattattaa gaatgcacag
     7741 attcctgcaa tgggtgcttc ttgtacaaac cccatggtaa cacagggcaa tgaattttac
     7801 ttccgtgcat gcttccttga ccccttccaa ggtaaggtta tggctacata tgcaattgag
     7861 cagggagcaa agacagcagc tgttatcatg caaaatggag atgactactc ggtaggtctt
     7921 gcaaacttct ttattaatgc atttaaagat ttaaccggag acgagaacag tatagttgat
     7981 gtttcggaat tccagttgaa tgaatctgac tttaatgcca ttcttacaaa tatcaaggca
     8041 aagaatcctg acgtaatatt tgctccaagt gcagcaacta cagctccatt aataattaaa
     8101 caagcaagag accttggaat cgacagcttg ataatgggcg gagatacatg ggaaaatcct
     8161 gccattattg atgttgccgg agaagatgcc gaaggtatag ttctttcgac tttctttgat
     8221 gaaaatgacc ctgctacgga agaagccagg aaatttgttg aaggctacaa aaaagaatta
     8281 gcaggtaaag agcctataat tcctgcagtt gcagcattag gctatgatgc atatatattg
     8341 ctcagagatg caatagaaag agcaggttca actgatggcg ttgctataag agatgcatta
     8401 aaagcaacaa aagattttga aggcgtaaca ggaattatta acttcactga agaaggagat
     8461 gctgataaga atcttgctgt tattaagaca gttaaggatg gacaatttgt gtttgtagat
     8521 tcagtagaag taaaataact gatatagaac tattagggcg gactgttcgt tcgccctttt
     8581 acagttttaa cagatatttg gaggaataat atgactggtg aaatttttat gcagcagctt
     8641 gccaacggca tttcattagg aagcttgtat gccctgttgg caataggtta tacgatggtt
     8701 tatggaattc taagacttat taattttgcc catggggata tttttatgat ggcagcgtat
     8761 tttatggtgt ttagcattat tacttttaat ataccatggt ttatatcaat tataattaca
     8821 ataatagcaa cggttaccct tggagtatta gttgaaaaaa cagcttatag accattaaga
     8881 aatgctcctc gtatgtccat tatgatttct gccataggag tatcatttct gttggaaaac
     8941 ttagcaacat atttgtttac aggtgttccc agagggtttc ccgacataaa agttttaact
     9001 acaacaatga aattaggaag tctttcatta accgttgcca cattgattac accttttgtt
     9061 acgcttatgc ttttatatgt tgtattgttt ataacaaata aaacaaaaat cggtatggca
     9121 atgagggccg tttcaaagga ttttgaaaca gccaaactaa tgggaataaa tataaatcaa
     9181 gtaatatcca taacttttat aataggttca tttttagcgg cgattggagc agtactgtgg
     9241 ggttcaagat atccttccgt aatgccgctc atgggtgtta tgcccggact taagtgtttc
     9301 atagcagcag tcttgggcgg tatcggaaat actacaggag ctgtattagg aggatttata
     9361 ctaggtatgg ctgaaattat gcttattaca tttctgccat ccctaaccgg ttacagagat
     9421 gcaatggcat ttgttatatt aataattgtg ttgttggtta aacctacagg gcttcttgga
     9481 gagaaggtga cggataaggt atgatggata aacttaataa taaaaataat aaattaattt
     9541 tgaatatatt gcttattatt atatttgcat cgataataat tcttttagat acaaaaaaaa
     9601 tcggtgatgc ctatataaga cgtattttaa atttatctgc aatatatgca atagtgagcg
     9661 tttcaatgaa cttggtcaac ggttttacgg gattattctc cttggggcag gcaggcttta
     9721 tggcaatcgg tgcttacacg gttgcaatat ttaccgttcc cttagccgcc agagctaaaa
     9781 tattttatat tactcctcaa aatcctgtgt tggcacaaat tgagctgcca tttgttattg
     9841 cacttatact gggaggtctt ttagcagcct tggtagcact tttaataggt ttacccgttc
     9901 tcaggttaaa gagcgactat ttagcaatag caacattagg attttcggaa ataatccgta
     9961 ttgtttttac aaacattaaa tcaattacca acggtgcatt gggaattaaa aatataccta
    10021 caatgcctac catgtgggtt ttctttggaa tagccataat ttcaatcggt tttatgtggc
    10081 tgttaataaa ctccagctac ggcagggctt ttaaagcgat aagagaggat gaagtggcag
    10141 cagaagccat gggtatcaat ttgtttaaac ataaagtaat gtcattcagc ataggagcat
    10201 tttttgcagg aatcggaggc ggactattgg ctacactgtt tgctacggta gaccctaatc
    10261 aattttattt ccctgttacc tacaacttcc ttttgattat cgtattggga ggtatgggaa
    10321 gtatatcagg tacgatcata gcatcctttg tagttacgtc aggacttgaa atactaagat
    10381 ttttcgatga acccctatca ttgtttggat taaatattcc attattccgt cccggtttaa
    10441 gaatggtaat attctcaata ttattgatgg tgttggtgct cttcttcaga aacggcctgt
    10501 taggacagaa ggaattgtcc tggaagttga tttttgacct gctttccggg aaaaaaccac
    10561 gaaaaaagac cggtgaagga ggtgctgcac aatgagtgtt ttaaagataa acaatgtaac
    10621 tatgcaattt ggtggagtta ctgctgtcaa caaccttaat attgaggttc aagagggaag
    10681 aattgaagcc ttgataggtc ccaacggtgc aggaaaaaca accgccttta atgttattac
    10741 cggtgtatat tctccaacta aaggaaatgt attttataaa aatgaaaaca tcacaggcct
    10801 tacacctgat aaaataacca aaaaaggtat agctcgcaca tttcagaata tcagattatt
    10861 caaaaattta accgtgcttg aaaatatact gattgccaat catttaagaa taaacagcaa
    10921 tttcctgtcc agcacactta gaatgccttg gtctaataaa gaagaaagac aaatgcgtga
    10981 aaaatcggag atgcttctag acaaattgga tttgttaaga ttgaaaaatg aaattgctca
    11041 ttctcttcca tatggtgagc agcgaaaatt ggaaatagcc agagctcttg caactgatgc
    11101 cgaccttctg ctcttggatg agccggctgc aggtatgaat ccctcggaaa caatggagtt
    11161 gtcatcattc attcataata taagagatga atttcatctt acgattttta tgattgagca
    11221 tcatatggac ctggttatgg aaatatctga aaaaatctac gttttagact tcgggcagct
    11281 tatagcagaa ggaacggcaa atgaagttaa aaatgaccaa agagttatag aggcttactt
    11341 gggggtggaa gaagatgctt aaaattaatg gactacatgt aaattacggg ggaatttccg
    11401 cagttaaggg tataagcata catgttccgg aaaaatcaat cgtaacatta ataggagcca
    11461 acggtgccgg taaaagcacc acccttaaag ccatagcagg actcgttaaa gcaaggaacg
    11521 gcgaaattat ttttcaaaat gaaaacatac tcggaaaaga tacaatagat attgtagcaa
    11581 gaggaattac catggttccg gaaggaagaa aagtctttgt gaatctttcg gtgctggaaa
    11641 acctaagagt cggtgcctac ttgagaagag acggaattga agatgatata aaaagagttt
    11701 atgatttgtt tccaatactc aaagaaagag cttggcaatt ggcaggtacc ttatctggag
    11761 gcgagcagca gatgctggca gtaggaagag ccttaatggc aaggcctaag ctgataatga
    11821 tggatgagcc ttccttggga ttagctccct taatagttaa ttcaatattt gaaattatta
    11881 aagaaataaa caaacaagga actacaattt tgttaatcga gcaaaatgca aacaaggcac
    11941 tgaaggttgc agattacggt tatgtcttgg aaacgggcaa tataaccatg gaaggaaaag
    12001 gaattgagct cctgaaaaac gagaaaatta tagaagcata tttaggaaca tcaaaaaaat
    12061 aaagaaacgg gacagtgggg aactacctta ctgtcccgat tttgtaatat ccaagatacg
    12121 tccccatttc cacgtcgttt cctgaatcaa aaaaacccat gtgggacagt aaggtacgtc
    12181 cccattgttt gaatcaaaga ccctgccaat acagaaaaaa catatatata caacataatc
    12241 ttgaggtttt cattaatatt tcgatttacc ttgaataaaa tcaacaaccc tactcctgct
    12301 cccgtagaga ggccggcaat taccgagcca aagcttatgc ttcctgcgat aaacagctga
    12361 gtgagtataa ttgatgcggc gcaatttggt ataaatccta ttatacctgc caaggcaggc
    12421 tgaaaaatac tgtcatgtag taaaatttca gataaattat cctcgcctac atactctatg
    12481 aaaaaattta taagaaaatt agtaataaac aaaaatataa atatattgat tgtatgcttc
    12541 aatgctggct ttaatatacc cgggtcgtca tggcatccgc agtcggtgca aacatggtga
    12601 tgaatagaac ttgcaatccc ttcattattg cttttattaa aaaaaccttt aaaaatgaaa
    12661 tccgccatag ctccggctat aacagcaaat attgctttta cggttataag caacaatata
    12721 acatttaaat taccgttact tgataataat atcggtattg cttcatcgga tgtggatatg
    12781 aatacagcta tcaatgttcc tgcagttata attctccctg catataaatt tgatgcagct
    12841 accgaaaatc cgcactgggg aacacctcca agcacagctc ccccgacaac cccaaacttc
    12901 tgaaggccac ttaccagctt atctgatgat ttatgctcta taaattcaat tataatatat
    12961 gctgcaaata aaaagggcag cattttgaat gaatccttaa atgtatgttc aagtgcatgt
    13021 tcaattactt cccacatatt tcctccggtt aaaatatagt atgattatat atgtgcacct
    13081 atttatt
//
LOCUS       DAOSHP010000052        12805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4102_length_12805_cov_10.401882, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000052 DAOSHP010000000
VERSION     DAOSHP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_12805_cov_10.401882"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLB33_06210"
     CDS             complement(<1..851)
                     /locus_tag="PLB33_06210"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOG63152.1"
                     /translation="MNKFEKISAMIDNTPMLEISLKYQGLRRKVYAKAEYYNFTGSIK
                     DRIAYNILKYSYEAGLIKKGDIIAEASSGNTGIAFSAMGAFLENPVHIFMPDWMSEER
                     KKLLESFGATIHLVSKKDGGFKGSVESADKFGAENNAFLPHQFSNLLNAETHYKTTGA
                     EILRQLDAIGKKPDGFVAGVGTGGTIMGVKNALQEKYPKCKAFPLEPKQSPAMSSGDM
                     VSGFHRIDGIGDGMIPEIVKLEQLDNVIRVDDGDAINMARQLALELGIGVGISSGANL
                     IGSIIAQ"
     gene            1024..2244
                     /locus_tag="PLB33_06215"
     CDS             1024..2244
                     /locus_tag="PLB33_06215"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOG63153.1"
                     /translation="MLIKMPKEVETALNILHVNGFEAYIVGGCVRDSIMGKVPSDWDV
                     ATSAKPEEILLCFSQYRTIETGIKHGTITVIINKMQIEITTYRIDGEYSDNRRPDNVL
                     FTDKIEFDLKRRDFTINALAYNKNGVIDLFGGLDDIKNKIVKCVGNSDERFNEDGLRI
                     LRALRFASVLNFDIDENTSCSIYGNKNLLNNISKERINIELNKLLMGSNFQNILLEYR
                     DVIEVFLPEIRDLTKEKFEQRLKAMKKLNSLVLRLTVLLYEIDSPDKILMNLKYDNKT
                     VKTVKLLAENIDEKIYPDRVNIKRWLNKINHVNLKYLINIKKALFDYEYEELAKSEVI
                     MKEIIETNQCYSLKTLAINGQDLIDSGMPKGKNIGIVLNDVLNKVIEGKLENKKDILF
                     NYIYDRRKEIETPN"
     gene            2351..>3450
                     /locus_tag="PLB33_06220"
     CDS             2351..>3450
                     /locus_tag="PLB33_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HOG63154.1"
                     /translation="MDKKLRDTGIEVIGEAFWGTHIAQFYESKDDFFSIGCPYIRAGL
                     LNNELCVWVYSNNTNEEEIKSYLEKYINNADSYIKAGQLLLEPHVDWYLTNNSFNEIR
                     VNEHWLECVTKSTKLGYEGLRAVGDTSWLEKCYYRTFANYENKINKMLPELPFIALCL
                     YDENKTDKLQYAEVIKNHSYTIINNENKKELITNIELVIKDKQLNKALEYNKVKAEFF
                     ANISHELRTPLNVILGTVQMMELAKCTSYEKEEKYLKFLKQNSLRLVRLISNLIDITK
                     IDANYFEIHRKNLDIVNFAELITFSVLDYAKCKNINIIFDTDIEEKVISCDPDYIERI
                     LLNLLSNAIKFTPRGGKILVSVHDMNERISISV"
     assembly_gap    3451..3550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3859..4332)
                     /locus_tag="PLB33_06225"
     CDS             complement(3859..4332)
                     /locus_tag="PLB33_06225"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOG63155.1"
                     /translation="MRFFYGERNILRALEEADFWKKQESEHVDVILEIVPDLEEEYIQ
                     KLEEYQEIFNSTHGRIVQLVETAVNCHNILSPELQRDIINIINLAVRQSQVFIDFIGM
                     LLRDSKAVTNNPTAIIVLNHIRRESEYFVGIVTAFLNSSCANNGRYYNDMGFQRK"
     gene            complement(4412..4633)
                     /locus_tag="PLB33_06230"
     CDS             complement(4412..4633)
                     /locus_tag="PLB33_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63156.1"
                     /translation="MIAIKGRRKCANCGKRYNYSYAKGEEASKKAKFKGKEENLSTVT
                     NVKVLSVHTFEVTATCPECGYEEVFIYND"
     gene            complement(4655..5383)
                     /locus_tag="PLB33_06235"
     CDS             complement(4655..5383)
                     /locus_tag="PLB33_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG63157.1"
                     /translation="MKITIIGHWGGFPKANEATSGYLIEHDNYKLLLECGSGVVSSLQ
                     KITDIKNLDAVLITHYHYDHCCDIGPLQYAIQIKTQLKEINAPLPIYAPTGEFFNLLK
                     WENYTYGISFEENSNLTLGPFEISFIKNIHPVEAYSVRIKCGDKVFSFTSDTSYFENL
                     AEFFSNSDLLICECSFYSDMDGLKAGHLNSSQAGMLAEKANVKKLILTHLPHFGDLNN
                     LLTEAKEFYKGEIELASYMMEIDI"
     gene            complement(5393..7108)
                     /locus_tag="PLB33_06240"
     CDS             complement(5393..7108)
                     /locus_tag="PLB33_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63158.1"
                     /translation="MLKRFIAYYKPVKIIFITDMICAFAVAVCDLFYPMITRNIINIY
                     VPNQEFQLMIKWLVVLGFIYILKFGLNYYITYYGHIMGVKMQAHMRKDIFEHLQDLPF
                     VFFDENKTGTLSSRIINDLMDISELAHHGPEDLFISLVMLIGSFIMLSTINLPLSLIV
                     FSAVPLLLIVAMKLRLKMEDAFMETRVTIGEVNATIENSISGIRVSKAFSNKENEIEK
                     FEKNNSRFQEARRKAYKVMAEFHVGSSFIIDILNIVVLSSGAIFFFIGIINFGDFAAF
                     LLYTGNFLNPIRKLVNFVEQYQSGSSGFKRFIEIMDKDVEKDDPGAADIYDVKGEISF
                     DDVSFAYDDNKEILKDITFDIEAGKTVAIVGPSGGGKTTLCHLIPRFYEIEEGSISID
                     NIDIRKFTRKSLRKNIGIVQQDVFLFTGTILENIQCGKLDATKEEVYKAAKNANIHDF
                     IMSLPEGYDTYVGERGVKLSGGQKQRISIARVFLKNPPIIILDEATSALDNATEFLIQ
                     KALDELSKGRTTIVVAHRLSTIKNADEIIVLTDQGIQEKGRHQELLDKKGMYAGLYNS
                     QFINQ"
     gene            complement(7112..7384)
                     /locus_tag="PLB33_06245"
     CDS             complement(7112..7384)
                     /locus_tag="PLB33_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63159.1"
                     /translation="MSKRPGYAATAAILGIIMHNASVHKIFERIFKYSAHKNAKAAMI
                     ISLILIAIISYFIRLGFILFVLLDFASIILFTAAALIYSKFENRQE"
     gene            7770..9266
                     /locus_tag="PLB33_06250"
     CDS             7770..9266
                     /locus_tag="PLB33_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63160.1"
                     /translation="MKKIIAMLLIAMLISACTAQENTPEKADVKTELTMGYTTEPEGL
                     DPHRTAAASTFTVTNNIYDTLVGVTPQWEIVPRLAKEWVISDDGMEITFSLRDDVYFH
                     NGRQMTAKDVEYSFTRLKDAESPRARDYENIVKIEVLNDYEIKFITAELDVELLKSFV
                     YPWAAVVPEEEVANLKTEPVGTGAFTLEEWVPQQHLILKRNENYYGDEIKLETVKMVL
                     IPDATGMMAGFQVGDLDIIPLTGDQVTMVEGNENYKIISEPMNAVQIMSLNTKNEYLS
                     NEKVRQAMAMAINKDEVIEASMFGYGAKIGSHLPPTSPDYYDTNNIIEYNPEKAKELL
                     KEAGYENGFEVDMSLPKNYQLHVDAGQVIADQLGRIGIKVNIQLVEWGTWLSEVYGAK
                     NFDTTVVGHTGRLDSYAFLSRYKSDSNDYISLTTGEVDALLEEALKELDENKRKEIYK
                     DIQVILANKLPAIYLQTPHTMLALQKNVEGMKIFPIDFYDYKDVYFTE"
     gene            9291..10250
                     /locus_tag="PLB33_06255"
     CDS             9291..10250
                     /locus_tag="PLB33_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63161.1"
                     /translation="MFNYVLDRLKSSIVVLFVVSIITFFVLMIIPGNPAQLILGADAT
                     PEAIQELHVAMGLDRPLYQQYFSWLLDLLKLDMGKSYVYGESVTVLIAKSIPVTFSLS
                     IFAMIMAAFIAFLFGMISAIKKDSIADYFSRSIMQLGAAIPSFWIGMVFIVYFGVKLK
                     LFPVSGFIPFEVNFFRFIKSITLPSLVLAIGEIGTLLRIIRSSMLDSLKQDYMDMARV
                     KGLSSFKIYIKYALRGALTAPLTIIGMQFAKLAGGTVVVETIFALPGLGRLVLTAVEH
                     RDIVLLQGLVMFITSTVIIITLIVDISVMFFNPRVKSFQQGDL"
     gene            10247..11065
                     /locus_tag="PLB33_06260"
     CDS             10247..11065
                     /locus_tag="PLB33_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63162.1"
                     /translation="MRKNISLILGIILVLIIIAVWVLSFVYLPYPPNEMNIPNKFLKP
                     GIDSEHILGTDNFGRDILSRIIYGSRNVLLVAISSVTLGALLGIFLGALAAMERFADT
                     IIMRIMDGLMAFPGILLAMMLVTVMERGIKGSIAAISIFMVPVFSRLVYSMILETEAL
                     LYIKAAKSYGSSRKQIFIRHYIPAMLPRLITQFTSAIGSAVMIETSLSFLGLGVQPPY
                     ASWGLMLSESRQFFLGYPYLAVGPGIAIIITVLGFNLIGDGLNDIIINRGNSDE"
     gene            11058..12008
                     /locus_tag="PLB33_06265"
     CDS             11058..12008
                     /locus_tag="PLB33_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63163.1"
                     /translation="MNETVVLNNLHIKAGSNTLVKNTSLIINEGEIFGLVGESGSGKT
                     ITAKAIINLLPKGLSYSADEIITAGKNMLTASAKEKKEHIGKNIGYIPQNTVFYLHPM
                     IKIKNQIGDGYIHHMNKTKKEALEKAEALLYKVGFKYPKIVLNYYPWQLSGGMRQRCN
                     IAMALMNDPKLIIADEPTTALDSTVQRQVMELFRKINEEMKLSILLISHDLGLVKHYS
                     HSLAVMYAGQIVESGYTNAVFSNPKHPYTQLLIKIIPSLKIKKNEPLTEIPGLISENG
                     RDIEGCLFFNRCPKAMDICKEMVAEHVDDKHEHYYRCNLE"
     gene            12017..>12805
                     /locus_tag="PLB33_06270"
     CDS             12017..>12805
                     /locus_tag="PLB33_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG63164.1"
                     /translation="MTKKIVISAVNIRKEYVVKKHLFTGKIKEKYVAVKGAAFDVEEG
                     KTIGIVGESGSGKSTIGEIVGGLQKPTYGCVLYFGKNIKELSEEEYHDYRKSVQFIFQ
                     DPKGSMDPNYEIIDILTMPLLTTNIAKNKDEAEKLIDKMLLRVGLDLTIKNKYPAEVS
                     GGQCQRAAIARALLVNPKLIICDEPVSALDVSIQAQILNLLRELQRELNISYIFISHD
                     IGVVNYMSDTILVMQNGNIVERGEAKEVLLHPKKEYTKILIESAF"
BASE COUNT         4204 a         2080 c         2315 g         4106 t
ORIGIN      
        1 ttttgtgcta ttatggagcc gattaagttg gcaccggaag aaatacccac accgattcct
       61 aactctaaag ccagctgtct tgccatgttg attgcatcac cgtcatcgac gcgaattaca
      121 ttatccagct gctctaattt aacgatttcg ggaatcatcc cgtcgcctat tccatcaatt
      181 ctatgaaagc ctgaaaccat atcgcctgat gacatggcgg gcgactgttt tggctcaaga
      241 gggaatgcct tacatttggg gtatttttct tgcagagcat ttttcactcc catgatggtc
      301 cctcctgtac ctactcctgc aacaaaacca tccggttttt tgccaattgc gtcaagttgt
      361 cttaaaattt cagctcctgt tgttttataa tgggtttccg catttaagag atttgaaaac
      421 tgatggggta aaaaggcatt attttcagct ccgaatttat cggctgattc gacagagcct
      481 ttgaaaccgc catctttctt tgaaaccaag tggattgttg caccgaaact ttccaatagt
      541 ttttttcttt cctcgctcat ccaatccggc ataaaaatat gtactggatt ttctaaaaat
      601 gctcccatgg cagaaaatgc aattccggtg ttgccgcttg atgcctcagc aattatatct
      661 ccctttttaa ttaagcctgc ttcataggag tattttaata tattgtaagc aatcctgtct
      721 ttaatgctgc cggtaaaatt gtagtattcc gccttagcgt atacttttcg ccggagtcct
      781 tgatatttta aagaaatttc cagcatggga gtattatcaa tcatagctga tattttttca
      841 aatttattca taataccata tcctttttat tattataact aacatttaaa ataatataat
      901 ttttaaattt aaatcacaaa atgcaacaaa atatttggaa attttatttt ttttcgacat
      961 tttttccttg ttaaaattac aggattggtt ataattatat aaaaaggtaa aaagaaggat
     1021 tttatgctta ttaaaatgcc aaaggaagtt gaaactgcat taaatatatt gcatgtaaac
     1081 ggctttgaag cttatatagt agggggatgc gtcagggaca gcatcatggg caaagttcct
     1141 tccgattggg atgttgccac atcagcaaag ccggaagaaa tactgctgtg ttttagtcag
     1201 tacagaacca tagaaacagg tataaaacac ggtacaatta cagtaattat aaataaaatg
     1261 cagattgaaa ttacaactta cagaattgac ggggagtaca gtgacaacag gcgccccgat
     1321 aatgttttgt ttacggataa aattgaattt gatttaaagc ggagagattt tacgattaat
     1381 gctcttgctt ataacaaaaa tggagtaatc gacttgttcg gaggactcga tgatataaaa
     1441 aacaaaattg taaaatgcgt ggggaattca gatgaaagat tcaatgagga cggtttgcgg
     1501 attttacgag cattgcgctt tgcttccgtt ttgaactttg acattgatga gaatacatca
     1561 tgcagtattt acggaaacaa aaatctccta aataatattt ctaaggaaag aattaatatt
     1621 gaattaaata agctgttaat gggaagtaat tttcaaaata ttcttttgga atacagagat
     1681 gtaattgaag tttttctgcc tgaaattaga gatttaacaa aggaaaaatt tgagcaacgt
     1741 ttaaaggcaa tgaagaagtt aaattctctg gttttgagac taactgtgct tttgtatgaa
     1801 attgattcgc ctgataagat acttatgaat ttaaaatatg acaacaagac tgttaaaaca
     1861 gttaagttgc ttgctgaaaa tattgatgaa aaaatttacc ccgaccgggt gaatattaag
     1921 agatggctca ataaaatcaa tcatgtaaat ctaaaatatc ttattaatat taaaaaggca
     1981 ttgtttgatt atgagtatga agagttggcc aagtccgaag taatcatgaa agaaattatt
     2041 gaaactaacc agtgttacag cttaaaaaca ttagcaatca acggccagga ccttattgat
     2101 tcaggtatgc cgaaaggcaa gaatatagga atagtattaa atgatgtatt aaataaagtt
     2161 attgaaggca aattggaaaa taaaaaagac atcttattta attacattta tgatagaaga
     2221 aaggagattg aaacaccgaa ttgacatgtt aagtagaaaa gtctatgcaa atattgaagt
     2281 aatctggaat acctgttaat cttcctaaat ttttatttaa atatattgtt gataaatttg
     2341 ggagtataaa atggataaga aacttaggga tacaggtata gaagtaattg gtgaagcttt
     2401 ttggggaact cacatagctc aattttatga atccaaggat gattttttta gtatcggttg
     2461 cccttatata agagcaggtt tattaaacaa tgaattgtgt gtatgggttt attcaaacaa
     2521 cacaaatgaa gaagaaataa aaagttattt agaaaagtat atcaacaatg cggatagtta
     2581 tattaaggca ggacagttgt tgttagaacc acatgttgat tggtacttaa ccaataatag
     2641 ttttaatgaa ataagagtta atgaacattg gcttgaatgt gtcacaaaat ctacaaaact
     2701 aggttatgaa ggacttagag ctgtcggtga cacatcttgg ctggaaaaat gttattacag
     2761 aacctttgct aattatgaga ataaaataaa taaaatgttg ccggaattac ccttcattgc
     2821 tttgtgccta tatgacgaga ataaaacgga caagctgcaa tatgcggaag ttatcaaaaa
     2881 tcacagttat acaattatca ataacgaaaa taaaaaagaa ttaataacaa atattgaact
     2941 ggtaattaag gataaacaac taaataaagc attggaatac aataaagtta aggcggagtt
     3001 ttttgccaac atttctcatg aattaagaac tcctttaaat gtcatattag ggacagtaca
     3061 aatgatggag ttagcaaaat gtacatctta tgaaaaagaa gaaaagtatt taaaattttt
     3121 aaaacaaaat tctctaagat tagtaagact aataagcaat ttaattgata taacgaaaat
     3181 tgatgctaat tattttgaaa ttcacagaaa aaacttagat atagtaaatt ttgcggaatt
     3241 aattactttt tctgttttag attatgctaa gtgcaaaaat attaatatca tttttgatac
     3301 agatattgaa gaaaaggtaa tatcatgcga cccggattat attgaacgaa ttttgctgaa
     3361 tctcttatct aatgctatta aatttacgcc gaggggcggt aaaatattag tatcggtgca
     3421 tgatatgaat gaaagaatca gtatttcagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn aataagctcg atgggaaaat atatttaaaa agccagcctt ccaagggcag
     3601 tgaattcatt gttgaaattc cctgcatgac aacacccgag acatcagaat ttaatattga
     3661 aaacacctca gtcaataata ttgtagaaag ggctaaaatc gagttttctg atatatattg
     3721 ttaactgata aagactttta cttgtccgga cggagttatt gttattcttg tgacgctaac
     3781 cccgcccatt ttttatgcct tcttatatat cccatcccct gtaataagtc cttcttttta
     3841 atccctataa ttgctttgtc attttctttg aaatcccata tcattgtaat atctgccatt
     3901 attagcacaa gagctgttta agaaagcagt tacgattcct acgaagtatt ccgattctct
     3961 tcttatgtga ttaagaacaa ttatcgccgt aggattgttt gtaacggctt tgctgtctct
     4021 tagcagcata cctataaagt caatgaaaac ctggctttgt cttactgcta aattaattat
     4081 attaataata tccctctgta attcgggaga taatatgttg tgacaattta cagctgtttc
     4141 cacgagctgg actattcttc cgtgtgtaga attgaatatt tcttgatatt cttctaattt
     4201 ttgaatatat tcttcttcta agtcaggaac tatttctaaa ataacatcaa catgttcact
     4261 ttcctgcttc ttccaaaaat cagcttcttc taaagccctt aatatattcc tttcaccata
     4321 aaagaatctc atcgtacatc ctccaaataa ttatttggta tattatacgt atgagattct
     4381 tttatgttat tattaaggtt aaataccttt attaatcatt atatatgaac acttcttcat
     4441 agccgcattc cgggcaggta gcggttactt cgaaggtatg tacggaaagc acttttacat
     4501 ttgtaacagt gcttaaattt tcctctttac ccttaaattt ggctttcttt gaagcttctt
     4561 cgcctttcgc atatgaataa ttatatcttt tgccgcaatt tgcgcatttt cgtctgcctt
     4621 ttattgctat catggtttca cttccctaag agtattatat atctatctcc atcatatatg
     4681 atgctaattc aatttcaccc ttataaaatt cctttgcttc agtcaataaa ttatttaaat
     4741 cgccaaaatg aggaaggtgg gtaagtataa gtttctttac atttgccttc tctgccaaca
     4801 ttcctgcttg agagctgtta agatgtcctg cctttaatcc gtccatatct gaatagaagc
     4861 tgcattcgca aatcaataaa tcagagtttg aaaaaaactc tgccaagttt tcaaaatatg
     4921 aagtatccga agtaaatgaa aatactttat cgccgcattt tattctaacg ctgtatgctt
     4981 caacagggtg aatattttta ataaagctta tttcaaatgg tcccagagtt aaattgctgt
     5041 tttcttcaaa acttatgccg taagtgtaat tttcccattt aaggagatta aagaattctc
     5101 ccgtcggtgc ataaatgggc agtggagcat taatttcctt aagctgcgtc ttgatttgta
     5161 ttgcatactg caagggtcct atatcacagc aatggtcata atgataatgc gttatcaata
     5221 cagcatcgag gttctttata tccgtaattt tctgcaggga gcttacaact ccgctgccgc
     5281 attctaaaag gagtttataa ttatcatgtt caatcaaata tcctgaagtt gcttcattag
     5341 ccttcgggaa tccaccccaa tgacctatta ttgttatttt cattatctct ccttattgat
     5401 ttatgaattg tgaattgtat aatccggcat acataccctt tttatccaaa agctcctgat
     5461 gtcttccttt ttcctgtatg ccttggtctg ttaatactat aatttcatcg gcatttttaa
     5521 ttgttgataa tctatgagct acaactatcg tggttcgtcc ctttgacaat tcatccaaag
     5581 ctttttgtat caaaaactcg gttgcattat caagagcgga ggttgcctca tccaatatta
     5641 ttataggcgg atttttaaga aatacccgag ctatggaaat tctttgtttt tgtcctcccg
     5701 acaattttac tcctctttca cccacataag tgtcataccc ttcaggcaaa gacattataa
     5761 aatcatggat gttggcattt ttggctgctt tgtatacttc ttccttggtg gcatcgagtt
     5821 taccgcattg aatattttca agaatagttc cggtaaacaa gaatacatcc tgctgaacaa
     5881 ttcctatgtt ctttctgaga gattttcgtg taaactttct tatatcaata ttatcaatgc
     5941 tgatacttcc ctcttctatc tcataaaaac gcggtattaa atgacaaagg gttgtcttgc
     6001 ctcctcccga tgggccaaca atggcaaccg tcttgcctgc ttcaatatca aatgttatat
     6061 cctttaatat ttctttatta tcatcataag cgaaagagac gtcatcaaaa cttatttcac
     6121 cctttacatc ataaatgtct gcagcgccag ggtcatcttt ttcgacatcc ttgtccatta
     6181 tttcgataaa tcgtttgaat cctgagctgc ccgattgata ctgctcaaca aagttgacta
     6241 attttcgtat agggtttaaa aagttccccg tatacaataa gaacgctgca aaatctccaa
     6301 agttgattat ccctataaaa aagaatatgg ctcccgagct taaaacaaca atatttaaaa
     6361 tgtcaattat aaatgaagag ccgacatgaa attctgccat taccttgtag gcttttcttc
     6421 ttgcttcctg aaatctgctg ttgttttttt cgaatttttc aatttcattt tccttattgc
     6481 tgaatgcttt ggaaactctt attcctgata tgctgttttc aatggttgca ttaacttcac
     6541 ctatcgtaac ccttgtttcc ataaaggcat cttccatttt gagtctaagc ttcattgcaa
     6601 caattaacaa aagaggaact gctgaaaata caatcaatga aagaggcagg ttgattgtgc
     6661 ttaacataat aaaagagcct ataagcatca ccaaagatat gaataaatcc tcaggtccgt
     6721 ggtgagcaag ttctgaaata tccattaaat catttattat tcgggatgat aatgttcccg
     6781 ttttgttttc gtcaaaaaat acaaagggca ggtcttgcag atgctcaaat atgtcttttc
     6841 tcatatgagc ctgcattttt actcccataa tatgaccgta atatgtaata taataattaa
     6901 gtccgaattt aagaatgtat atgaatccta atacaacaag ccattttatc ataagctgaa
     6961 attcttgatt cggtacatag atgtttatta tgtttcttgt tatcatggga taaaacaaat
     7021 cacatactgc tactgcaaat gcacaaatca tgtcggtaat aaaaattatt ttcaccggtt
     7081 tataataagc tataaatctt ttaagcattt tttactcctg tcgattttca aattttgaat
     7141 atattaatgc tgcagccgta aataaaatta tagaagcaaa atccaataat acgaaaagta
     7201 tgaatcctaa tcttataaaa taacttataa tggcaattaa aattaaagaa attatcattg
     7261 cagcttttgc atttttgtga gcactatatt tgaaaattct ttcaaatatt ttatggacac
     7321 ttgcattgtg cataataatt cccaagattg ctgccgttgc tgcatatccc ggccttttag
     7381 acattaatga gcaaccttgc gaaaatacaa atattccaat tcccaatata aagtggtaaa
     7441 aaaacctgtg ttcacgcatt tgctttcgct gttgttgacg taattcgtac ataatatcac
     7501 ctctatttgt ttagataata atatcattta ttgctaattt tttcaacgaa caatttatgc
     7561 atatcctcta ttgactttat aaagaaaata tgttaaacct agaaaaggaa gagggacata
     7621 ccggtccctg ccaaataata atatgttttc aaggatttat tacagatacg tatgatttga
     7681 ttataaagat ttgaaaacgg gtatattatt attggcattc agtaaaagat attctaaatg
     7741 aaattttatt aactgaaagg aaaagaacaa tgaaaaaaat tatagcaatg cttttaattg
     7801 caatgctgat ttcagcatgc acagctcaag aaaacacacc tgaaaaagca gatgttaaga
     7861 ctgaacttac catgggatat actacggagc ccgaaggtct tgaccctcac agaactgctg
     7921 cagcttccac atttactgta accaacaata tatatgacac tttggtaggc gtaacgccgc
     7981 aatgggaaat tgtgcccaga ctagcaaagg aatgggttat ttctgatgac ggaatggaaa
     8041 ttacattctc attgagggat gacgtttatt ttcataacgg aagacagatg actgccaaag
     8101 atgtggagta ttcatttacc aggcttaagg atgctgaaag ccccagggca agagattatg
     8161 aaaatatagt aaaaattgaa gttctaaacg actatgaaat aaagtttata actgcagaat
     8221 tggatgttga gcttttaaag agctttgtat atccatgggc agccgttgtt ccggaagaag
     8281 aagttgcaaa cttaaaaacc gaacctgtcg gaacgggagc ttttactctg gaagaatggg
     8341 tacctcagca acatttgatt ttaaaaagaa atgaaaatta ttacggtgat gaaataaaat
     8401 tagaaaccgt taagatggtt ttaatacctg atgctaccgg catgatggcg ggatttcagg
     8461 taggagatct tgacattatt ccattaacgg gagatcaggt aacaatggtt gaaggaaatg
     8521 aaaattataa aataatttct gagcctatga atgccgttca aataatgtct cttaatacaa
     8581 aaaatgaata tctttcaaat gaaaaagtca gacaggcaat ggcaatggct ataaataaag
     8641 atgaggttat tgaagcttcc atgtttggat atggtgctaa aatcggctca cacctgcctc
     8701 ctacatcacc tgattattat gacacaaata atataataga gtataatccc gaaaaggcta
     8761 aagagctttt gaaggaagca ggatatgaaa acggatttga ggttgatatg tcacttccca
     8821 agaattatca gcttcatgtt gatgcggggc aggtaattgc agaccaattg ggaaggatag
     8881 gaataaaagt gaatattcag cttgttgagt ggggaacatg gttaagcgaa gtatatggag
     8941 ctaagaactt tgataccacg gttgtcgggc atactggaag acttgattca tatgcatttc
     9001 tttcgagata taagtcagac agcaacgatt atataagctt gactacagga gaagttgatg
     9061 cgcttttgga agaagctctt aaagaattgg atgaaaataa aagaaaagaa atttacaaag
     9121 atatacaggt aattttagca aataagcttc ctgcaattta tttgcaaact cctcacacaa
     9181 tgttggcact tcaaaaaaat gttgaaggaa tgaagatatt tcctatagac ttttatgatt
     9241 ataaggatgt gtattttaca gaatagctaa attgccctct gaggtgtctt atgtttaatt
     9301 acgttttaga tagacttaaa agctcaatag ttgtattgtt tgtagtgtcg attattacgt
     9361 tttttgtgtt aatgataata cccggaaacc ctgcacagtt aatattggga gcggatgcaa
     9421 ctcctgaagc gattcaggag ctgcatgttg ctatgggact tgacagaccg ctctatcagc
     9481 aatatttcag ctggcttttg gatttgctaa aacttgacat gggtaaatcc tacgtctatg
     9541 gtgaatcggt tactgtctta atagcaaaaa gtatacccgt aactttttca ttatcaattt
     9601 ttgctatgat tatggcagca tttatagcat tcctttttgg aatgatttcg gcaatcaaaa
     9661 aggacagtat tgcagattat ttttcaagaa gcattatgca gcttggagca gccattcctt
     9721 ccttctggat aggaatggtt tttatagttt actttggtgt gaagctaaaa ctatttcctg
     9781 tttcaggctt tattccattt gaagtcaact tttttagatt tataaaaagc ataactttac
     9841 cctctcttgt tctggctatc ggagaaatag gaacactttt aagaattata agaagctcca
     9901 tgctcgattc tcttaaacag gattatatgg acatggcaag agtaaaaggg cttagcagct
     9961 ttaaaatata tataaaatat gccttaaggg gtgcattgac tgccccattg acaataatag
    10021 gaatgcagtt tgccaagctt gcgggaggaa cggttgtggt tgaaacaata tttgcccttc
    10081 cgggacttgg aagactggtt cttactgccg tagagcacag ggatattgtg cttttgcagg
    10141 gtcttgttat gtttatcaca tcaacggtaa taattatcac tttaattgtt gatatatcgg
    10201 taatgttttt taatccaagg gttaagtcct ttcaacaggg ggacctatga gaaaaaatat
    10261 aagcttaatt ttaggaatta tattagtgtt gattattata gctgtttggg tactatcatt
    10321 tgtttattta ccctatcctc caaatgaaat gaacattccg aacaagtttt tgaagccggg
    10381 cattgattca gagcatattc tgggtaccga taatttcggc agggacatac taagccgtat
    10441 aatatacggt tcaagaaatg tcttgttagt tgcaatatca tctgtaacac tgggtgcctt
    10501 gctcggaata tttttgggag ctcttgctgc catggagcgc tttgcagata caataattat
    10561 gcgtattatg gacgggctta tggcttttcc gggaatactt ctcgcaatga tgctggtgac
    10621 tgttatggaa aggggaatta aaggctcaat agcagcgata agtattttta tggtacctgt
    10681 attttcaagg cttgtgtatt ctatgatatt agaaactgaa gcacttttgt atataaaagc
    10741 tgcaaaaagc tacgggagtt caagaaaaca aatattcatc agacactaca tacctgcaat
    10801 gcttccaaga ttaatcaccc agtttacttc agctataggc tcggcagtta tgattgaaac
    10861 atctctttct tttttgggac ttggagttca gcctccatat gccagctggg gcttgatgtt
    10921 aagtgaatca agacagtttt ttttgggtta tccgtatttg gctgttggcc ccggaattgc
    10981 aataataatt acagtgttag gatttaacct tattggagac gggttgaacg atattattat
    11041 aaacaggggg aattctgatg aatgaaactg ttgtgcttaa caatctccat ataaaggcag
    11101 gaagtaatac attagtaaag aatacttcac taattattaa cgaaggagaa attttcgggc
    11161 ttgtaggaga atcaggctcc ggaaaaacta ttactgctaa ggctattatc aacttgcttc
    11221 ctaagggatt aagctattcg gctgatgaaa taattactgc cggtaaaaat atgttaactg
    11281 cttcggcaaa agaaaaaaaa gaacatatag ggaaaaatat tggatatatt cctcaaaata
    11341 cggtatttta tctgcatccc atgattaaaa taaaaaatca aatcggagac ggatatattc
    11401 atcatatgaa taaaaccaag aaggaagctt tggaaaaagc agaagctttg ttatataagg
    11461 ttggatttaa atatcccaaa atagtgctga attattatcc gtggcaatta agcggaggga
    11521 tgcgtcaaag atgcaatatt gcaatggctt tgatgaacga cccgaagctt attattgcag
    11581 atgagccgac aacggctttg gacagcactg ttcaacgtca ggttatggaa ttattcagaa
    11641 aaataaatga agagatgaaa ttgtccatac ttttgatttc tcatgactta ggattggtaa
    11701 aacattacag ccacagctta gcggtaatgt atgccggtca gattgtagaa tcaggataca
    11761 caaatgcagt tttttctaat cccaaacacc cttacacaca gcttctgatt aaaatcattc
    11821 cttcattgaa aattaagaag aatgagccat taacagaaat accgggtctt atatcggaaa
    11881 atggcagaga tatcgaagga tgtttgtttt tcaacagatg ccccaaggca atggatatat
    11941 gtaaagaaat ggttgctgag catgtagatg ataagcacga gcactattac agatgtaatt
    12001 tagaatagag gcaattatga caaaaaaaat tgtgatatct gcagtaaata tcagaaaaga
    12061 atatgttgtt aaaaagcatc tttttacagg caagataaaa gaaaaatatg ttgccgtaaa
    12121 aggtgccgcc ttcgatgttg aagaaggaaa aaccatcggg attgtaggag aatcgggaag
    12181 cggcaaatct accataggcg aaatagtagg agggcttcaa aaacctacat atggctgtgt
    12241 tctttatttt ggcaagaata ttaaagaatt atcggaagaa gaataccatg actatagaaa
    12301 aagcgtacag tttatttttc aagaccccaa aggctctatg gaccctaact atgaaattat
    12361 cgatattttg actatgcctt tattaacaac taatattgca aagaataagg atgaagctga
    12421 aaaacttatt gataaaatgc ttttaagagt aggtcttgat ttaaccataa agaataaata
    12481 tcctgccgaa gtaagcggag ggcagtgcca gagggcagca atagcaagag cattgcttgt
    12541 taatccaaaa ctaataatat gcgatgagcc tgtgtctgcc ttagatgtat ccattcaggc
    12601 acagatactg aatttactga gggagcttca aagagagctt aatatttcat atatattcat
    12661 atctcacgat atcggagtag ttaattatat gtccgacaca atattagtta tgcaaaacgg
    12721 aaatattgtt gaaagagggg aggctaagga agttctcctg cacccgaaaa aagaatacac
    12781 aaagattctc atagaaagcg cattt
//
LOCUS       DAOSHP010000053        12284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4339_length_12284_cov_8.664159, whole genome shotgun sequence.
ACCESSION   DAOSHP010000053 DAOSHP010000000
VERSION     DAOSHP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4339_length_12284_cov_8.664159"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLB33_06275"
     CDS             complement(<1..868)
                     /locus_tag="PLB33_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOG63165.1"
                     /translation="MIRILKRTGNFVDFNADKISNAIMKAMKETKDGVDEELAKEISL
                     KIEEELSSKNYPIPVETVQDMVENYLMDSVRKDVAKKYILYRYERDKSRDSRKRKDSK
                     LLSEEFISKYKHRGSPMDQLGNFVYYRTYSRWLPEERRREYWWETVRRAVEYNCSLVP
                     TKREEAEQLYDNIFNLRQFLSGRTFWVGGTPVSYNYPMANFNCAFEIINDFHAFRDLF
                     YLLMIGSGVGVRILKSDIEQLPKIRGNYKIIHEDYTPVEKSKREDNTGVEFNQNNSVK
                     ITVGDSKEGWVQS"
     assembly_gap    1087..1186
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1187..1713)
                     /locus_tag="PLB33_06280"
     CDS             complement(<1187..1713)
                     /locus_tag="PLB33_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG63166.1"
                     /translation="MSLIPKELHPSWNIFLTDDIILELEDIERKIGNNINPAPENVLR
                     FMICNLYDIKVVILGQDPYPETGRATGRAFEVGDLTSWNQKFRQVSLKNIIRLIYKNY
                     NGISEYSDIKKFSEIQKEIKEGKFSILPPHKIFKSWEKQGVLLLNTYLSVEAGITGSH
                     ISIWQKFSEELLKFIS"
     gene            complement(1726..2076)
                     /locus_tag="PLB33_06285"
     CDS             complement(1726..2076)
                     /locus_tag="PLB33_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG63167.1"
                     /translation="MDRKKELKEQYKQMKTEMGIFIVQNKVNNKYLLVTTQNLHGMIN
                     RVRFQLNSGGHPNRELQKEWKEFGEKNFDIRILEKLAYDKDESKTDYSEELKIMDIIW
                     SEKQSYKNMEAYNK"
     gene            complement(2078..2566)
                     /locus_tag="PLB33_06290"
     CDS             complement(2078..2566)
                     /locus_tag="PLB33_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HOG63168.1"
                     /translation="MKIPTVLKHKPVIVSENYENVDGRNAYKSDAKGLSLGLAQWNDR
                     GKVDISAKVWRYTGEKWSRQSEELPLHRVLDLAILILRALLHFREAYRYEKLYDAENT
                     VIDRVGMQGDAMTVKVCTENEKIDEDIKLFNQALSDDGEIIGERLAILARLLNEMGYG
                     GR"
     gene            complement(2761..3522)
                     /locus_tag="PLB33_06295"
     CDS             complement(2761..3522)
                     /locus_tag="PLB33_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOG63169.1"
                     /translation="MEYTVKKLSLLAGVSARTLRYYDEIGILKPARINSSGYRIYGKE
                     EVDRLQQILFYRELDIGLDSIKKILSSPDYDDIVALREHKRALLAKREQLNILIDNLD
                     KTIKYREGRIVMNDSEKFKGFKKQLIEENEKKFGQEIREKYGEEAVKKSNEQFMNMSE
                     EQYKEFEELGNSIFEYLKKAMETNDPAGELAQKTAELHKKWITFCWGSYDKEAHAALA
                     QMYVDDERFKENYEKVQKGAAEFLRDAIFIYTGRK"
     gene            3680..8527
                     /locus_tag="PLB33_06300"
     CDS             3680..8527
                     /locus_tag="PLB33_06300"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOG63170.1"
                     /translation="MKKLLCLISLMCLILLSCKKTDVLPGLDTSPVEYGVDDKYELIA
                     LKSDKQGIDSTTVFQLTSKEEINNNFIKNNMQIVPEQEYKIEKLSGTTYNIIPAVNLE
                     NDKIYQIVLNDEGYEYSWAFQTKKKFVVESTLPTDRSGYVPSNSGIEIYFSLSGLEEL
                     DDFFEIKPHVDGKFIYKNNTAIFVPDKLEENINYRVTIKKGYGLKDSEQKLEEDYSFS
                     FNTKKEKYAEIYFDRPLTNIYENNAKIIEAYINNENLEFDINIYQYSNADYFAENIND
                     FAETGKFFTGSVDDIEGLSLLNTIKQKPNISYKNIYNTPKGLFEIPGLPKGYYLLEFS
                     TNSGEKNYHFLQINDMLIYYALFENQIVVIASNASDSKGIVDAGVVLNGEHLGFTDND
                     GILIADRDTSKYENKTISLRVEAKGFSDFVYADSFYFFNYYYRSQYEAYKYIRYIDTD
                     RPVYLPTDKINVWGFARHRDDKSVNKVRIELVEANTGLVLDEKTVDLTNIGTYQTEFE
                     IKNITSDILQINVYDNDLKISTKYVSIGEYTKPLYTVKGEFDKKFVYKGESVNYKVKA
                     SFFDGYPVPNLELKFGTFSSNYSGSLEYGGMDTTVKTDEKGETVISVNTNLTSDSWRP
                     VSIRASLWNAKAEDKPVSLIDGFEVFPHHEMVEIVQNDVEHPENISILLHELDITNYD
                     SQYFGGYEKLRGNPLSGTVQVSVAESYYKKVKKGEYYDFINKVNVVDYTYNYVENVVF
                     NEITEITDGYGNLQIPDFNPERTYKISAEYMSENGPVREWAHVGYYSRYYEKLYYSLE
                     KDKDKDNYRLNDIVSLKLTYNHEDVQNTDYDKLILLIMKNGLIDYKITDNTNLSFEFE
                     EEYIPNVMLNSIYYKNGYMYPVEFTGSLLYDTTEKQIHFDVTTDKEEYRPGEEVVLSI
                     KTKDENNKPITADVNISVVDEAYFALFEKRVDTLNSLYARVSSGLVATYLSNIDLSMD
                     NGSGAERGGGGEDYTNFRDEFKDTNVFKTITTDKNGNGELKFKLADNLTSWRITYQGI
                     SDKLFAGSGTKNITASLPFFVDVIMSKEYLKDDKISASLRVFGAEAKESEEVNYEIKI
                     INKETGKELKYKETGIIGDYTNISFDKLDEGSYEIYIYASYKDFEDGIMEEFNVLDSF
                     VYFNNTTYYKLTEDTVLDSVYSNPVITLYNESRSDFYKSLENILANSGKRIDQTVCSM
                     LAAKYINDYFKTDLYFNEDELINEVNKYEIDTWGGYSLFTYSAADVEITAKLINLIDN
                     EYIGYKSKTYFKNILQNPDYNEEIPAALWGLSTYKEPVLLTIYDLLENNTLEIRDKIY
                     LSLALAELGDLNTAKKYYKEFSNSFKESGNYLYFESGADENSSSENYELTALLSLLGC
                     KLKDYETSDKLFKYIYNNPSKFTLSSFEQLIYIMNRDIMKLDEIKDLFGEVTVNSEGK
                     DKTYKLKLFDRESFSVPKDKIKDVKFKNIKGSIACMVEALGNKDDLEKNKTDDFSIKI
                     DYSLKDSTQNQMSYNHSDLVKVTIMPSFSKNVITGNYEITYVVPAGFRFIELDRKNPF
                     WVEENGQKLRFFFHYDRQNFAAIPITFYIQAAQKGEYTVDYVVIKENLEPKLNYLDKT
                     ILSVK"
     gene            8560..9444
                     /gene="amrB"
                     /locus_tag="PLB33_06305"
     CDS             8560..9444
                     /gene="amrB"
                     /locus_tag="PLB33_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG63171.1"
                     /translation="MNKFFKVFTVFLLFITIAGSILAYNTAKNRHEKYFFDGRKNIEC
                     LYYDPSDFINIPEYETSQDIGTIHGCIVPHHLVAKDLIHEVFQNVQKNNYKTVVLIGP
                     DHESTNKGKIFTTLSNWQTPRGVLETDGNLTKELLKYDFVTEDDDKITMEHSTSSIIP
                     FINYYLNDIKIVTLVLTKQVKLEDVEILTDELYKNINLHETLFIASVDFSHYLNLEDA
                     QKKDLISMEAIKNKDINKLMSFTNDNLDSPISVVTLLKMMDKLGAAGDTVLNHSNSEL
                     ILKKKIEETTSYITYLFY"
     gene            9562..10227
                     /locus_tag="PLB33_06310"
     CDS             9562..10227
                     /locus_tag="PLB33_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63172.1"
                     /translation="MQIYLKKFIRLIFGLYIYALGLVLCIYANIGLAPWDAFAIGISK
                     VTGISYGNVSILIGITIVIALFFLLKEKIGFGTILNAILIGFFSDLIIDSKIIPYMQN
                     FFTGVLMLVTGQILACIASYLYIGVGLGAGPRDSLMIGLGKKFSNIPIGIVRGSIEAT
                     VLFIGWILGAKVGLGTVIYVFSIGFLLQTTFKLLKFDVKSVVHESVFDTINIFKKIHS
                     ENI"
     gene            10239..10838
                     /locus_tag="PLB33_06315"
     CDS             10239..10838
                     /locus_tag="PLB33_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOG63173.1"
                     /translation="MCGRYYIDIDNEEFKKILEEAQRNIYENFKSGEIFPTNIAPIYI
                     EDDNKIKPLLAKWGFPKWDGKGVIINARAETLNEKLMFKRLSSSNRCIVPASFYYEWK
                     QEEKGKHKYKINKPGSNIYMAGLYNIAANKNKQLSLFDENIDIYYTIITRNSNDSVSH
                     IHSRMPLIFSIEEMNEWLRGQSIDELMKSEHMLTGEITE"
     gene            complement(10904..11233)
                     /locus_tag="PLB33_06320"
     CDS             complement(10904..11233)
                     /locus_tag="PLB33_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG63174.1"
                     /translation="MEIITSYENLQELIEKNDMVMIYFGSKTCSVCKSMTPKVENMIK
                     RYPNIYAIMVEADKSLDISSKFNVFTVPVILLYIEGKETIREVRIISLDNLEQKIARY
                     CQIFYVH"
     gene            11385..11684
                     /locus_tag="PLB33_06325"
     CDS             11385..11684
                     /locus_tag="PLB33_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOG63175.1"
                     /translation="MIDFSNKERVKKVLSEKAELFKSIAHPVRLCILAMLIKEEESNV
                     TDIQCCLDVPQPTVSQHLSKLKSAGILSAERNGTEIYYKIINEEVKKIVSQILDA"
     gene            11695..>12284
                     /locus_tag="PLB33_06330"
     CDS             11695..>12284
                     /locus_tag="PLB33_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG63176.1"
                     /translation="MSKKILIIGGVAGGASAAARLRRLDETSKIIMFEKGPYISYANC
                     GLPYHIGKVIKERSSLLLQTPEAMSKKYNIDVRVESEVISINRESKTLDVKNLKTGEI
                     YTESYDTLIISTGSSPLKPPIEGINLPGIFTLWTVPDTDNIIKFINEKKPKNALVIGG
                     GFIGLEVAENLVSVGLNVTVAEAMNQVMAPIDYEMA"
BASE COUNT         4205 a         1918 c         2008 g         4053 t
ORIGIN      
        1 gggattgaac ccatccttcc ttgctgtcgc caacggttat ttttacggaa ttattttggt
       61 tgaattcaac gcctgtgtta tcttctctct tggacttttc tactggggtg taatcttcat
      121 ggattatttt ataattccct cttattttag gcagctgctc tatatcgctt tttaaaattc
      181 ttacacctac tcccgagcct atcattaaaa gataaaataa gtccctgaat gcatggaaat
      241 catttattat ttcaaatgca cagttgaaat ttgccatagg atagttatat gaaaccggag
      301 ttccgccaac ccaaaatgtt ctgcctgaca aaaactgtct taaattaaat atgttatcgt
      361 acagttgctc tgcttcttct cttttcgtag gtactaaact gcagttgtat tcaacggctc
      421 ttcttaccgt ttcccaccaa tactcccttc ttctttcttc aggaagccat cttgaataag
      481 ttctgtaata aacaaagtta ccaagctgat ccataggtga gcctctatgc ttatacttgc
      541 tgataaattc ttcgctaaga agtttggaat cctttctctt cctggagtcc ctagacttat
      601 ccctctcata tctgtataaa atgtatttct ttgcgacatc ctttctgaca ctgtccatga
      661 ggtagttttc aaccatatcc tgtaccgttt ccacaggtat gggatagttt ttacttgaaa
      721 gctcttcttc aattttcagg cttatttcct tagctaattc ctcatcaacg ccatcctttg
      781 tttctttcat agccttcata atagcattgc tgattttatc ggcattaaaa tcgacaaagt
      841 ttccggtcct ttttaatatt ctaatcatat cccttttctc cctgttattt tatgtggtac
      901 tacatattgt agcagtaaca ctaaaatatg tcaatcaaat actatatata gatatttttt
      961 gcacaaatgg acaatatcat tgaaatatca ttgttttaaa aataatatcg aaatttgcaa
     1021 aaaatttaaa aaaattcctt tgttgaaatt tcaaggaatt tttatattat taaaagattt
     1081 taacatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaaa taaattttag
     1201 taattcttca ctgaatttct gccatatgga tatatggctt ccggtaatgc ctgcttcaac
     1261 cgataaatat gtgttaagta aaagcactcc ttgtttttcc caagatttaa aaattttatg
     1321 gggaggaaga attgaaaatt ttccttcctt tatttctttt tgtatttctg aaaacttctt
     1381 aatatcacta tactcactta ttccgttata atttttgtaa atcagtctga taatattttt
     1441 caaagatacc tgcctgaatt tttgattcca ggatgtcaag tcccctactt caaatgcacg
     1501 acccgttgct cttccggttt caggataagg gtcctgtccc aatattacga cttttatgtc
     1561 ataaagattg catatcatga aacgcagaac attttcagga gcaggattta tattgttgcc
     1621 aatctttctt tctatatctt ccaattctaa tataatatca tcggttaaaa atatattcca
     1681 ggaaggatgc agttcctttg gtattaaact cattctttca cgtccttatt tgttatatgc
     1741 ttccatattt ttatacgatt gtttttcaga ccatattata tccattattt ttaattcttc
     1801 agaataatct gtttttgact catccttgtc atatgccaac ttttcaagaa ttctgatatc
     1861 aaaatttttt tctccaaatt ccttccattc cttttgcagc tctctattgg gatgtcctcc
     1921 gctgttaagc tgaaacctta ctctgttaat cattccatgc aaattttgag tagtcactaa
     1981 aagatattta ttgttgactt tattctgcac gataaatatc cccatttcag tcttcatttg
     2041 tttgtattgt tcttttaatt ctttttttct gtccatatta tctgccgcca tatcccattt
     2101 catttaataa tcttgctaag atagccaatc tttctcctat tatttcccca tcatcactaa
     2161 gagcctgatt aaacaattta atatcttcat ctattttttc attttcggta catactttta
     2221 cagtcattgc atctccctgc atacctaccc tgtcgataac agtgttttct gcatcataca
     2281 acttttcata cctatatgct tctctaaaat gaagcagggc ccttaaaatc agtattgcca
     2341 aatccaaaac cctgtgcagg ggaagctctt ctgactgtct tgaccacttt tctcccgtat
     2401 atctccacac cttcgcagat atgtctacct ttcccctgtc attccattga gcaagtccca
     2461 gtgacaaacc ctttgcgtca gatttataag catttcttcc gtctacattt tcataatttt
     2521 cagaaacaat taccggttta tgttttaata ctgttggtat tttcattttc agtctccctt
     2581 caatgactta ctaatttact aaataactaa tttactaata ttaatatact actaaatatg
     2641 ttgagtgtca atatttattt attaataatg ttgcatacaa aaaagcgact aatatgtcac
     2701 tttttgtatg ggattatttt tcataatcgg tatgtttcac aacaccgata atatttacta
     2761 ttatttcctt cccgtgtaaa tgaatattgc atcacgcaaa aattctgcag ctcctttttg
     2821 taccttttca taattttctt taaatctttc gtcatccaca tacatctgag caagagcagc
     2881 atgtgcttct ttgtcgtaag agccccagca aaaggtaatc cacttcttat gaagttcagc
     2941 tgttttttgt gcaagttctc ctgcagggtc atttgtttcc attgcctttt taagatattc
     3001 aaaaattgaa ttgcctaatt cttcaaattc cttatattgc tcctcagaca tgttcataaa
     3061 ctgttcattg gattttttca cggcttcttc accatatttt tctcttattt cctgcccgaa
     3121 tttcttttca ttttcttcaa ttaattgttt cttgaatcct ttaaattttt cgctatcatt
     3181 cataacaatt ctcccttctc tatacttaat cgttttatcc agattatcaa ttagaatatt
     3241 taattgttct ctctttgcaa ggagggctct tttatgttcc ctaagagcaa caatatcatc
     3301 ataatcaggt gaagaaagta ttttcttgat actgtctaaa ccaatatcta attccctgta
     3361 aaacaatatt tgctgcagcc tgtctacttc ctcttttccg tagatgcgat atccggatga
     3421 gttaattctt gccggcttaa gaattcctat ctcatcgtaa taccttaagg tcctggcgct
     3481 cacacctgcc agcaaactaa gtttcttaac tgtatactcc ataatttcac tccttacaaa
     3541 tatattgtat acctttacgt aacgtcaatg tcaatatatt aattctataa aaagattatt
     3601 tattccggat ttcttgaact gtcttgtaac accatatgat aagtgatata attgacatat
     3661 aatctttggg agggaagaaa tgaaaaaatt attatgctta atctcattaa tgtgccttat
     3721 tttgttgtct tgcaagaaga ctgatgtgtt gcccggatta gatacttcgc cggttgaata
     3781 cggtgttgac gataaatacg agcttatagc ccttaaatca gataaacagg gaattgacag
     3841 tacaactgtt tttcagctga cttcaaagga agaaataaac aataatttca taaaaaataa
     3901 tatgcaaata gttcccgagc aagagtataa aattgaaaaa ctttcaggta caacatataa
     3961 tattattcct gctgtaaatc ttgaaaacga caagatatat caaatcgtac tcaatgatga
     4021 aggatatgag tactcctggg catttcaaac taagaaaaaa tttgtagtgg aaagtaccct
     4081 ccctaccgat agaagcggtt atgtaccttc aaattcaggt attgaaatat atttcagctt
     4141 aagcggttta gaagaattgg atgatttttt tgaaattaag cctcatgtgg atggtaaatt
     4201 tatctacaaa aataatactg ctattttcgt tcccgataaa ttagaagaaa acatcaatta
     4261 tagagtcaca ataaaaaaag gttatggtct caaggacagt gagcagaaat tagaagaaga
     4321 ttattccttc agctttaata caaaaaaaga aaagtatgct gaaatttatt ttgacaggcc
     4381 attgacaaat atttacgaaa acaatgcaaa aataattgaa gcatatataa ataatgaaaa
     4441 tctggaattt gacataaata tttatcagta ttcaaatgca gactattttg cagaaaatat
     4501 caatgatttt gcagaaacag gcaaattttt taccggcagt gttgatgata ttgaaggatt
     4561 atccctgtta aatactataa agcaaaagcc aaacatttct tataagaata tttataatac
     4621 tccaaaagga ttattcgaaa ttcccggcct tccaaaggga tattatttat tggaattttc
     4681 aacgaattcg ggtgaaaaaa attatcactt cctgcaaatt aacgatatgc taatttatta
     4741 tgctttattt gaaaatcaaa ttgttgtcat tgcatcgaat gcatctgaca gcaagggtat
     4801 tgtggatgca ggtgtggtat tgaacggaga gcatttaggt tttaccgaca atgacggcat
     4861 acttatagcg gatagagata cttctaaata tgaaaacaaa acaatttctt taagagttga
     4921 ggctaagggt ttcagcgatt ttgtatatgc agacagcttt tattttttca attattatta
     4981 cagaagtcag tatgaagctt acaaatatat aaggtatatt gatacggaca ggcctgtcta
     5041 cctgcctacg gataaaatca atgtatgggg atttgcaagg catagggatg ataagtctgt
     5101 taataaggta agaattgaat tggttgaagc taatacgggc ttggtgttag atgaaaagac
     5161 agtcgactta acgaatatag gaacttacca aacagaattt gaaataaaaa atattacttc
     5221 tgatattctt caaataaatg tgtacgataa tgatttaaaa atatccacaa aatatgtatc
     5281 catcggtgaa tatacaaaac ctctttatac ggtaaagggg gaatttgata aaaaatttgt
     5341 ttacaagggt gaaagtgtaa actataaagt aaaggcctct ttctttgacg gctatccggt
     5401 accgaactta gagcttaaat tcggcacatt ttccagtaac tacagcggaa gtttagaata
     5461 cggcggaatg gatacaaccg ttaaaactga tgaaaaggga gaaacagtaa taagtgtaaa
     5521 tacgaattta actagcgaca gctggagacc cgtttcaata cgagcaagtc tttggaatgc
     5581 aaaagcagag gataagcctg tgagccttat cgatggattt gaagtattcc ctcaccatga
     5641 aatggttgaa attgtgcaga atgacgtgga gcatcctgaa aatataagta tattactgca
     5701 tgaattagac attaccaatt atgacagcca atatttcggc ggatatgaaa agctgagggg
     5761 taatccctta agcggtacgg tacaagtaag cgtagcggaa agttattata aaaaggtaaa
     5821 aaaaggtgag tattacgatt ttataaataa ggtaaatgtt gttgattata catataacta
     5881 tgtcgaaaat gttgttttta atgaaattac ggaaataaca gacggatacg gcaatttaca
     5941 aattcctgat tttaatcctg aaagaacata taaaatttca gcagaatata tgagtgagaa
     6001 tggtcctgta agagagtggg cacatgtagg atactattca cgttattatg aaaaattgta
     6061 ttactcctta gaaaaggata aggataaaga taattacaga ctgaatgaca ttgtaagctt
     6121 aaagctcaca tacaatcatg aagatgttca aaacacggat tatgataaac ttatactgtt
     6181 aattatgaaa aacgggctta tagattataa aatcactgac aatacaaatc tcagctttga
     6241 atttgaagaa gaatatatac cgaatgtaat gctaaacagc atatattata aaaacgggta
     6301 tatgtatccg gtggaattca caggctcttt attgtatgat acaaccgaaa agcaaataca
     6361 ctttgatgta acaacggata aggaagaata cagacccggt gaagaagttg tattaagtat
     6421 aaaaacaaag gatgaaaata ataaaccaat tactgcagat gtaaatatat ccgttgtaga
     6481 tgaggcatat tttgcattgt tcgaaaaaag agtggatact ttgaattctt tatatgccag
     6541 ggttagctca ggacttgttg ctacttatct gtctaatatt gatttaagca tggataatgg
     6601 cagtggtgct gaaaggggcg gaggaggaga agattatact aatttcagag atgaattcaa
     6661 ggatacaaat gtgtttaaaa ccataacaac agataaaaac ggcaatggag aattaaaatt
     6721 taaattagcg gataatttga catcctggag aataacctat caagggattt cagacaaatt
     6781 atttgcagga agcggcacaa aaaatattac cgcaagtttg ccgttcttcg tagatgtaat
     6841 catgagcaaa gaatatctga aggatgataa aatatctgcc tccctaaggg tattcggcgc
     6901 tgaagcaaaa gaatcagaag aagtaaatta tgaaattaaa attataaata aagaaaccgg
     6961 taaagagttg aaatataagg aaacaggtat tataggagac tatacgaata tttcatttga
     7021 taaattagat gaaggctctt atgaaatata tatttatgct tcttataagg attttgaaga
     7081 cgggataatg gaagaattca atgttcttga ttcattcgta tattttaaca atacaactta
     7141 ttataaattg acggaagata ctgttttgga cagcgtatat tctaacccgg taattacctt
     7201 gtataacgaa agccgctccg atttttacaa gagcctggaa aatattttag ctaacagcgg
     7261 aaaaaggatt gaccaaactg tatgttcaat gttggcagcc aaatatataa atgattattt
     7321 taaaaccgat ttatatttta atgaggacga actaatcaat gaagtcaaca aatatgaaat
     7381 cgatacatgg ggtggataca gccttttcac atattccgca gctgatgttg aaataaccgc
     7441 aaaacttata aacttgatag ataatgaata tatagggtat aagtcgaaaa cttactttaa
     7501 aaatatattg caaaatccgg attacaacga agaaattccg gcagctcttt ggggcttaag
     7561 cacctataag gagccggtat tattaacaat ttatgattta ttggaaaaca atacattgga
     7621 aataagagat aaaatttatc tttccttagc tttggcggaa ttgggagact taaatacggc
     7681 taaaaaatat tataaagaat tttcaaacag ctttaaagaa tccggaaatt atttatattt
     7741 tgaaagtggt gcagatgaaa atagtagttc cgaaaattat gaattaacag cactattgtc
     7801 gctattggga tgcaagctta aagattatga aacaagcgac aaattgttta aatatattta
     7861 taataatcct tctaagttta ccctttcaag ctttgagcag ctaatatata ttatgaacag
     7921 agatataatg aaattagatg aaattaaaga tttgttcgga gaagttactg ttaattcaga
     7981 aggtaaagat aaaacatata aattgaaatt atttgacagg gaaagttttt ccgtcccaaa
     8041 ggataaaatt aaggatgtca aatttaagaa tataaaaggg agtatagcat gcatggttga
     8101 agcacttgga aataaggatg atttagaaaa aaataaaacc gatgatttct ctataaaaat
     8161 agactatagt cttaaagatt ccacacaaaa ccaaatgtca tacaatcatt ccgacttagt
     8221 taaagtaacc ataatgccat cctttagcaa aaatgtaata acaggaaatt atgaaataac
     8281 gtatgtggtt cctgccggtt tcaggtttat tgagcttgat aggaagaatc cgttttgggt
     8341 tgaagaaaac ggtcaaaaat taagattttt cttccattat gacaggcaaa actttgctgc
     8401 aatccctatt acattttata ttcaagctgc acaaaaggga gagtatactg tggattatgt
     8461 ggttataaaa gaaaatttgg agcctaaatt gaattatctt gataagacaa ttttatcagt
     8521 gaaataaaat tccggataaa tctaagtggc aggtgcataa tgaataaatt ttttaaagta
     8581 tttactgtat ttttattgtt tattacgatt gccggaagta tacttgctta taatacagcg
     8641 aaaaacaggc atgaaaaata cttttttgac ggaagaaaaa atattgaatg tttatattac
     8701 gatccatcag attttattaa tattcctgag tatgaaacaa gtcaagatat cggtactata
     8761 catggatgta tagtacctca tcatttggtt gcaaaagatt taattcatga agttttccaa
     8821 aatgtgcaaa aaaataatta taaaacagtg gtattgattg gtcctgacca cgaaagtacc
     8881 aataaaggaa aaatatttac gacattaagc aattggcaaa caccaagagg tgtcttagaa
     8941 acagacggta atttaacaaa agaattatta aaatacgact ttgtaacaga agatgacgat
     9001 aagataacaa tggaacattc cacctcaagt attattccct ttattaacta ttatctgaat
     9061 gatattaaga tagttacctt agttttgaca aagcaggtga aattagagga tgttgaaata
     9121 ttgacggatg aattatataa aaatataaac ttacacgaaa cattgtttat tgcctcagtt
     9181 gatttttccc attatttaaa tttggaagat gctcaaaaaa aagatttgat aagtatggaa
     9241 gccataaaaa acaaagacat aaacaaactc atgtctttta ccaatgataa tttagactca
     9301 ccgatatctg ttgtcacctt gctaaaaatg atggacaaat tgggagcagc tggtgatact
     9361 gtactaaatc acagcaattc agagctcata ttaaaaaaga aaatcgaaga aacaacaagc
     9421 tatataacat atttgtttta ttaagatact ttgacatcat ccacacacat attgtatacc
     9481 ttttatttac aatatttaaa aatttgttgt tatttaatat aaattcatat aaaattagtt
     9541 tattgaaatc aggaggatgc tatgcagata tatttaaaaa aatttatcag attaatattt
     9601 ggtctttata tatatgcttt gggtttagtt ttatgtattt atgccaatat tggacttgct
     9661 ccctgggatg cctttgcaat aggcatcagt aaagtaacgg gaatatctta cggtaatgta
     9721 tcgattctta taggtattac aattgtaata gcattatttt ttctattaaa agaaaaaatc
     9781 gggttcggga caattttaaa cgccatattg ataggattct tttctgattt aattattgat
     9841 tcaaaaataa taccatatat gcaaaatttt ttcacaggtg tattaatgct tgtaacaggc
     9901 caaatattag catgcatagc ttcatattta tatattggtg tgggtcttgg agcaggaccc
     9961 agggattctt taatgattgg tttagggaaa aaattttcta atattccaat aggtatagta
    10021 agaggctcca ttgaagcaac tgttttgttt ataggctgga tattgggagc taaggttggg
    10081 cttggaacgg ttatttatgt tttcagcata ggctttttac ttcaaactac atttaaattg
    10141 cttaaatttg acgttaaatc ggttgttcat gaaagtgtat tcgatacaat aaatatattc
    10201 aaaaaaattc actctgaaaa tatttaaatg aggataatat gtgcggaaga tattacattg
    10261 atattgataa tgaagaattc aagaaaatat tagaagaggc acaaagaaat atttatgaga
    10321 attttaaaag cggtgaaatt tttcctacaa acattgcccc tatttatata gaggatgata
    10381 ataaaataaa gcctctctta gctaaatggg gatttccaaa gtgggacggc aagggtgtaa
    10441 taattaatgc aagagctgaa acactaaacg aaaagctaat gtttaaaaga ctgtcttcat
    10501 ccaacaggtg tattgtgcct gcttcttttt attatgaatg gaagcaggaa gaaaaaggca
    10561 agcataaata taaaataaat aaacccggct ccaatattta tatggccgga ttatacaata
    10621 tagctgcaaa taaaaacaag caattgagct tgtttgatga aaacattgat atttactata
    10681 ccattataac acgaaattcc aatgattcgg tttcccacat acacagcaga atgccactca
    10741 tttttagtat tgaagaaatg aatgaatggc tgagaggaca aagtatcgat gaattgatga
    10801 aatctgagca tatgctaacc ggagaaatta ccgaataatt gacttcacta tatctaagct
    10861 ttcatttctc aaatgatatg attaaccata ctgaatattg aaattaatga acataaaata
    10921 tctggcaata ccttgcaatt ttttgttcta aattatccaa gcttattatt cttacttccc
    10981 tgattgtttc tttaccttca atatataata gtattaccgg cacggtaaac acattaaatt
    11041 tggaagatat atctaaactc ttatccgctt caaccattat tgcataaata ttaggatatc
    11101 ttttaatcat attctccact ttaggtgtca tggatttaca taccgagcat gtcttgcttc
    11161 caaaataaat cattaccata tcgttctttt caataagctc ttgtaaattt tcataagatg
    11221 ttataatttc catagtactc ctcattatta atgttcttat ttacatttta ccattatttc
    11281 ataaaattaa ccattttttt aaaaacaata aagtttttct tgcgtattat attattatat
    11341 gctaatattc taatatatta atattgcaat atatggaggg taaaatgata gatttcagca
    11401 ataaggaaag agttaaaaag gtattgtcgg aaaaagcaga gctgtttaaa tcaatagcac
    11461 atcccgtaag actttgcata ttggcaatgc ttataaaaga agaggaaagc aatgttaccg
    11521 atattcaatg ctgtttggat gttccgcagc ctactgtatc acagcatctt tccaaactta
    11581 agtcagcagg catactttca gcggaaagaa acggaacgga aatttattat aaaattataa
    11641 atgaagaagt aaaaaaaata gtaagccaaa ttttagatgc ataagggagg aattatgagc
    11701 aagaaaattt taataatagg cggtgttgca ggaggagcat ccgcagcagc cagacttaga
    11761 agacttgatg aaacatcaaa aattataatg tttgaaaaag ggccttatat atcgtatgca
    11821 aattgcggtt tgccttacca tataggaaaa gttataaaag aaaggtcatc cttgttgttg
    11881 cagactcccg aagctatgtc aaaaaaatat aatattgacg tgcgtgtaga gtctgaagtt
    11941 atttcaatta acagagagag caaaacccta gatgttaaga atttaaaaac cggcgaaatt
    12001 tatactgaaa gctatgatac cttgattata tccaccggct catcgccctt aaaaccgccg
    12061 attgaaggaa ttaatttacc gggtattttt actttgtgga ctgtgcccga tacagataat
    12121 ataataaaat tcataaatga aaagaaacct aaaaacgctc ttgtaatagg gggtggattt
    12181 atagggcttg aggttgcgga aaatcttgtg tctgtgggat taaatgtaac tgttgccgaa
    12241 gcgatgaacc aggttatggc tcctatagat tatgaaatgg caca
//
LOCUS       DAOSHP010000054        11985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4503_length_11985_cov_7.727745, whole genome shotgun sequence.
ACCESSION   DAOSHP010000054 DAOSHP010000000
VERSION     DAOSHP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11985
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4503_length_11985_cov_7.727745"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..1685
                     /locus_tag="PLB33_06335"
     CDS             348..1685
                     /locus_tag="PLB33_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOG63177.1"
                     /translation="MKKIVFILSLVLLLALSSVNAFADDVIINIDSTKVEFNEDLGFP
                     FVDENNRTQVPFRATLEKYGAVVEWDQETSTAIATKDEIVVKVPIGENYILKNDEKID
                     VDTAARIVNGRTYLPIRAVIEAFGSEVEWDGALKTVVITTVPVDAEAIYFAASDKSYE
                     WDNYDINAKINMSLNAPDGAGNVQPINMDMDMNMTIFMNPVKAKISTSMLIPGMEALG
                     QKTFMEMYMTIDEEGITQYIGMPGQTEELTWMKQTIANEMLAGFMKNNEETIEKNKEL
                     VKKYTDDVKYFGKYIEDGKTLLRLQYILSGDIYKEMFADFSEVVPEPATEEEAMALEM
                     IQNIANMDIGNLTYIIYIDEATGEMVKLEMDIAEMIASMVSGMAEITGMPPEAMEVLN
                     SLKAYMTVEFLNINSAEDFEIPEEALNAPDMLEGLQQQLETEIEIEFEAEEEL"
     gene            complement(1843..2418)
                     /locus_tag="PLB33_06340"
     CDS             complement(1843..2418)
                     /locus_tag="PLB33_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOG63178.1"
                     /translation="MYKRILNEILLSEIPSEGIYKLMDSGEMNNILPELLRLKGFEQQ
                     TPYHDKDVLEHTLAVVDEIKPKLNLRLAALLHDISKPDCFTVDENGRGHFHGHHVKSA
                     LASEKILQRLGYEEELILNVTILIRYHYIKDIAKVIKEKGIKKFVENVGAERLDDIFE
                     LIRADMTGKASANYEVIEKLRDMCNKYEEKQ"
     gene            2611..4320
                     /locus_tag="PLB33_06345"
     CDS             2611..4320
                     /locus_tag="PLB33_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOG63179.1"
                     /translation="MKRTVKIIITSFIVLIILTLNVYGLSFEASNHYELENIILEQMR
                     EYNPVFNIKYTGSLDNIEEVLKSMIDKDTYLKSNITRVDWDISGNKTASNINVRVSYI
                     MTKEERIEADKMIDEILADIIKPYMNDHEKVKAVHDYIVLNGKYDNNSLYFSDYDLLT
                     KGTSVCNGYALLTYNMLNKLNIPVNLVSGTSAGEAHIWNMVKLDDYWFHLDVTWNDPV
                     SDRDAVFYTYYMLTEKEICKDHAIDANLKIPKSTKEYYDYLVELSYNKLLVETGLDMY
                     NEENFAADESELKNLLTRKITHHPLMITVRFDKSISQDSIINAMSQLYKYDYISVINY
                     SLIDNDSKGEWNILNIFIKYKETPDNITLDFARSVYNTATEVDYNVYAQYGNKKINIT
                     KDVYIYPYDTNKINVSKGTLKFKEPGNYNLTFEYQGLRETVSITGLNSNAFEYITDKK
                     PDNYVNVKVYDQYIDFSSINQWPIIENDRTMVPLRAVFEVLNCNVKWEESSKSAVVEH
                     GSTKIIIPANSTTAYINGKANSLDVPAKIVNDRIMIPLRFVSEAIEKTVIWDDPNKTV
                     LIY"
     assembly_gap    4338..4437
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4438..5657)
                     /locus_tag="PLB33_06350"
     CDS             complement(<4438..5657)
                     /locus_tag="PLB33_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOG63180.1"
                     /translation="MVSKRKYILTIVAVVLVTAFLTLTLGNVLLVEIGQKVFLSKDEY
                     SQLKKISEKYAKQESIMKIAQNEFLYEADEEVMLEGALQGTLKALGDPYTQFLTKEEF
                     ESLMQDTEGSYEGIGVYITAGDDNKIIIVSPIEDTPAEKAGLRTGDKITRINGIEYTA
                     DQIDAAVKVMKGEPGTSVTLTIQRAASDGTQDISDVVINREKIRIKTVKPAMLGDDIG
                     YIRVTTFDMQTAKDFKAAYSTLKNRGLKGLVIDLRFNPGGMISTTVEIADMFMGEGIV
                     TYTKTKAGEIEYFKSDDKMEDIPIVILINEGSASASEIMAGAMKDTNRAVLIGQKTFG
                     KGIVQKVQRFGNDGEGIKMTISEYFTPAGINIHGVGIEPDIEIQLPEDAEGYGYDFYE
                     TDTQLQKAVEVLRK"
     gene            complement(5897..6661)
                     /locus_tag="PLB33_06355"
     CDS             complement(5897..6661)
                     /locus_tag="PLB33_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOG63181.1"
                     /translation="MIKNKLKEKLNAGKAAIGTFIVCNAPDLVEIAGLAGFDFVIIDN
                     EHGPMRAESSQHLIRAAEVRGITPIVRIPDRQESTILHTLDIGAHGIQVPQVNDVEAA
                     KEIIERSKYQPVGKRGLAFPRSADYGLCDISNYTDNANKETLIITHCENKTCLDNLEE
                     ICKIPEIDVIFLGPYDMSQSMGVTGQVTHELVESAAEKVLEVTKRYNKIAGIFAGNGE
                     TARKRAEQGFLYIAVGMDTTLFSAKCIEEISKFHNC"
     gene            complement(6658..7872)
                     /locus_tag="PLB33_06360"
     CDS             complement(6658..7872)
                     /locus_tag="PLB33_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOG63182.1"
                     /translation="MSKYAKRMKTMEEPANIIKGLFSSMNDPGVISFGGGAIAKECLP
                     VDIVREITSDIMTVEKRGYEILSYGPVIGVKDLRDVIVKELLAPKGVKANADNIIITT
                     GGLEAMSLMCQLFIEPGDVILVESPTFVHSVETFSMFEAKCVAVEMDDDGMVTEELEE
                     KIIKYNPKLVYVIPTFQNPTGRTLSLERRQKIAELGSKYDVIILEDDPYRDIRYSGED
                     LLPIKAFDNTGHTVLANSFSKIFSPGSRIGYVYANDDIISKMTDAKSASNSHTSLLPQ
                     IICAEFFKRGYYPEHHKLLCDIHRERRDAMLESIDKFFPEGTKRTFPDGGLFTWVELP
                     GGINTSELLKESVSNPEVKVAFVAGEGFFTEGKGKGSNCMRLSFGANPPDKIRIGIER
                     LGNLIKSKLEEK"
     gene            complement(8047..8592)
                     /locus_tag="PLB33_06365"
     CDS             complement(8047..8592)
                     /locus_tag="PLB33_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG63183.1"
                     /translation="MDKIIKVIKRYRFFIILLVINTIILIVKPDIGLKSLQLTKSNAL
                     EMLSILPPVFILLGLLDVWVKRETMIKFMGEKSGIIGVLLAFLLGSAAAGPLYAAFPV
                     AAVLLKKGSKFSNVLIMIGAWSTTKIPLLLFEASSMGIKFMITRFILNIFGIAIIAYT
                     VEKLLSKREIDKIYNNAASME"
     gene            complement(8592..9068)
                     /locus_tag="PLB33_06370"
     CDS             complement(8592..9068)
                     /locus_tag="PLB33_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOG63184.1"
                     /translation="MTTIGLYLITGTALVVSFIKNKDKTILSLKKAWKSFENILPQFL
                     TILVIIGIALAILTPEQISRLVGSESGWIGVLVAAFIGSITLVPGFIAFPLASALLKS
                     GAGYMQIAAFISTLMMVGVVTIPLEIKYFGKKTAIIRNMEAFIFSLAVAYIMGVVM"
     gene            complement(9398..11155)
                     /locus_tag="PLB33_06375"
     CDS             complement(9398..11155)
                     /locus_tag="PLB33_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOG63185.1"
                     /translation="MKKYARVISFILCCLFVISSVGCNQTVDKEQPPEPAESNDAANE
                     TETLFKSGTYTGISKGRNGPIVVQIDFSDDTIDDVRVISHRETYNVGDVPIKTYPKII
                     VENQSLDVDIVSGATISSVAFMEAVNQCVKEAGADPAQLKEPIPVTEKAEDTTADIVV
                     IGGGSAGMTAAIYGANAGRKVILVEKLGFVGGTSAFSIESFGSSETSVHKGLGITATS
                     EQNYESFVKANPKGDPEAFRILADNNGKAADWLKSIGAPLSVTNNPFSVTSNREAGPL
                     GLVITSALAAEMKKAGVDIRVNTKATEIIMEDGKIAGVKVENPAGEYVIKSKAVIIAS
                     GGFAANNEMVSKYMPELKGYNSSCSIGAKGDGHLMAEALGAELWDMDYIRVNFTYYTD
                     GIRYYYIGCLPNNGAIFVNNEGERFINDQAAYGAGKTVVDQGGTGWMIFDQSMIDSIE
                     EVREYYELGLFESAPTIGELADKIGVNKDNLIATVEKYKGFVEKGVDEDFGRKMLNLT
                     FDEAPFYACKMTCHVQGTFGGIKTDTNAQVLKTDGTIIPGLYAAGECASAGTYGANPA
                     AVNIVFGKIAGENAANYSE"
     gene            11490..11963
                     /locus_tag="PLB33_06380"
     CDS             11490..11963
                     /locus_tag="PLB33_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOG63186.1"
                     /translation="MFREMRRKDREIKKDEAIEILKNNNYGILSTISQNGYPYGVPIS
                     YTYINGSIYFHCAAEGHKLDNIKNNDKVSFCVVGQTKTLPDIFSVKYESVIVFGRVVE
                     VYGDEKNMALLEILNKYSPDYIEQGLEYIQNSSEDTKVIRINVEHISGKARKQTQ"
BASE COUNT         3862 a         2202 c         1811 g         4010 t
ORIGIN      
        1 acatattatt cttatacctt ttttatactc gtttaaactt tattttcatt atatgttatt
       61 atccttgaat aaagatatta atttgctcta tcctaatatt agacttattg attcttaaat
      121 gaagaatatg atatgttaat tataacaaat ataaaactat ttattacaat ttacatattt
      181 attgttgaaa ctttaacata tatattgtat cataagggca agccgcaaaa tcggcttact
      241 acataccgat acaatgaata catcggttca aagaaccaag tctttgattc attattgtat
      301 ttattatatc ataaaaattg gaaaaataat aaatttagga ggaaattatg aaaaaaatcg
      361 tatttatttt aagtttggta ttgcttttgg cattatcatc ggttaatgct tttgcagatg
      421 acgtcataat aaatatcgat tccaccaaag tcgaatttaa tgaggattta ggttttccgt
      481 ttgttgatga aaacaacaga acacaggtac ctttcagagc tactcttgaa aagtacggtg
      541 ccgtggttga atgggatcaa gaaaccagta ctgcaattgc aacaaaggat gagatagttg
      601 taaaagttcc tattggtgaa aattacatac tgaaaaatga tgaaaaaatt gatgttgata
      661 ctgctgcaag aatcgtaaac ggaagaacct accttccgat aagagcagtc atcgaagctt
      721 tcggctcgga agttgaatgg gatggagcat taaaaactgt tgtaataaca acagtgccgg
      781 tagatgcaga agccatatat tttgcagcaa gcgataaatc ctatgaatgg gataattatg
      841 atattaatgc aaaaataaat atgtccctta atgctcccga tggtgcagga aacgttcagc
      901 caatcaatat ggacatggac atgaatatga caattttcat gaatcctgta aaggctaaaa
      961 ttagtacatc aatgctgata cccggaatgg aagctcttgg ccaaaagaca tttatggaaa
     1021 tgtacatgac aattgatgaa gaaggtataa ctcaatatat aggtatgccg gggcaaacag
     1081 aagaactgac atggatgaaa caaacaatag caaatgaaat gttagccggc tttatgaaaa
     1141 ataatgaaga aacaatagag aaaaacaaag aattagtcaa aaagtatact gatgatgtta
     1201 aatacttcgg aaaatatatt gaagatggaa aaacattgtt aagacttcag tatatattat
     1261 caggagacat atataaagaa atgtttgctg atttttcgga agtagttcca gagcctgcaa
     1321 ctgaagaaga agcaatggcg cttgaaatga tacaaaacat tgcaaatatg gatattggaa
     1381 atttaacata catcatatat attgatgaag caaccggtga aatggtaaaa ttagaaatgg
     1441 atatagcaga aatgattgca tctatggtat ccggtatggc agaaataacc ggtatgcctc
     1501 ctgaagcaat ggaagtttta aacagcttaa aggcttatat gacagtggaa tttttaaata
     1561 tcaacagcgc cgaagatttt gaaattccgg aagaagcttt aaacgcaccg gatatgcttg
     1621 aagggttaca acaacaatta gaaaccgaaa ttgagataga atttgaagct gaagaagaac
     1681 tgtaatgagt agaaacgatg aaaagtgcac ttttcggtgc acttttttta ttgattatat
     1741 tttatgaaat gttagtataa ttaatagcaa atcaaagctt ttgaaatcct tgggtataaa
     1801 tttaaattta agccctgttg ctgatgtatc tacaaatcat tcctattgtt tttcttcata
     1861 cttattgcac atatctctta atttttcaat tacttcataa tttgcagaag ctttgcctgt
     1921 catgtcggct ctgataagtt caaatatatc gtcaagtctt tctgccccaa cattttctac
     1981 aaattttttt attccttttt cctttataac tttggctata tcctttatat aatggtatct
     2041 tattaaaatt gttacattca gaattaattc ctcttcatat ccaagtcttt gaagaatttt
     2101 ttcacttgca agagcagact ttacatgatg cccgtgaaaa tgacctcttc cgttttcatc
     2161 aaccgtaaaa caatcaggct tactaatatc gtgcaaaaga gctgccaatc ttaaattaag
     2221 tttcggttta atttcatcta ctacagccaa tgtatgctct aatacatcct tatcatgata
     2281 aggtgtttgt tgctcaaagc ctttaagcct taaaagttca ggaagtatat tgttcatttc
     2341 accgctatcc attaatttat atattccttc tgagggtatt tcacttaaca atatttcatt
     2401 taaaattctt ttatacatat ttctcctttt atgaaacttt ttctaaaatt ttacgtctaa
     2461 ttaataatac ggcataaaac gctcacccgt tgttgtttag tgtgtacgga aacaattata
     2521 ttacattcga ttgtaattgt aaaatataat actatttatt ttatgtaaaa tggtatataa
     2581 tcataccaga agagtcagga aggtgattta atgaaacgaa ctgttaaaat aattataacc
     2641 tcttttatag tcttaattat tcttactctt aatgtttatg gtttgtcttt tgaagcaagc
     2701 aatcattatg agcttgagaa tattattctt gagcaaatga gggagtataa tccggttttt
     2761 aatattaaat ataccggctc cttggacaat attgaagaag tattaaaaag tatgatagat
     2821 aaggatactt atttaaaatc taacataacg cgggtggatt gggatatatc aggcaacaag
     2881 actgcaagca acataaatgt aagagtttct tatattatga ctaaggaaga aagaattgaa
     2941 gcagataaaa tgattgacga aatcttggct gatattatta aaccttacat gaatgaccac
     3001 gaaaaggtta aagcagttca tgattacata gtattaaacg gtaaatatga taacaactct
     3061 ttatattttt cggattatga tttgctgacg aaaggaactt cagtttgcaa cggatatgct
     3121 cttttaacct acaatatgct taacaagctt aatattccgg ttaatttggt atccggtact
     3181 tccgcgggag aagctcatat ttggaatatg gtcaaattag atgactactg gtttcactta
     3241 gatgtaacat ggaatgaccc ggtgtcagac agagatgcag ttttttacac atactacatg
     3301 ctaacggaaa aagaaatttg taaagatcac gcaattgatg caaatttgaa aatacctaaa
     3361 tcaaccaagg aatattatga ctaccttgta gaattatcct ataacaaatt gttggttgaa
     3421 acagggcttg atatgtataa tgaggagaat tttgctgcgg acgaatctga gttaaaaaat
     3481 ttactgactc gtaaaattac acatcatcct ctaatgatta ccgtaagatt tgacaagtca
     3541 atttctcaag acagtataat taatgctatg tctcaattat ataaatacga ttatatatcc
     3601 gtaattaatt acagtctgat tgacaatgac agtaaaggcg aatggaatat tttaaatata
     3661 tttattaagt ataaagaaac acctgacaat ataactttag actttgcaag gagtgtttac
     3721 aatactgcaa cggaggtaga ctataacgtc tatgcccaat atggcaacaa aaaaataaac
     3781 attacaaagg atgtatatat ttatccctat gatacaaata aaataaatgt ttcaaaagga
     3841 actttaaaat tcaaagagcc cggaaactac aacttaacgt ttgaatacca gggacttcgt
     3901 gaaacagtat ccataacggg attaaattca aatgcatttg aatacattac tgataaaaaa
     3961 ccggacaatt acgttaatgt taaagtttat gaccaatata tagatttcag ttcaattaat
     4021 cagtggccta ttatagaaaa tgacaggact atggttccat taagagccgt ctttgaagta
     4081 ttaaactgta atgtcaagtg ggaagaatct tctaagtctg cagttgttga gcacggctca
     4141 acaaaaataa ttatacctgc aaactctacc acggcttata ttaacggcaa agctaattct
     4201 ttggacgttc ctgcaaaaat agttaatgac cgcataatga taccccttag atttgtcagc
     4261 gaagctattg aaaaaacagt tatttgggat gatccaaaca aaacagtgct gatttattag
     4321 gaggtagcaa catgcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat
     4441 tttcttaata cttcaactgc cttttgaagc tgagtatccg tttcataaaa atcatatcca
     4501 tagccttctg catcttcagg taactgaatt tcaatatccg gctcgattcc tacgccatga
     4561 atgttaattc ctgccggtgt gaagtattct gaaattgtca ttttaattcc ttcaccatca
     4621 tttccgaatc tttgaacctt ctgtactatt ccttttccaa atgtcttttg ccctattaag
     4681 actgccctat tggtatcctt catggctccg gccataattt cagaagcact tgcacttcct
     4741 tcatttatta atataacaat aggaatatct tccatcttat catctgattt aaagtattcg
     4801 atttctcctg cctttgtctt agtataggtt actatacctt cacccataaa catatctgct
     4861 atttcaacag tggtgctaat cattcctccc gggttaaacc ttaagtctat gaccaaccct
     4921 ttcaaacctc tattttttaa agtcgaatat gcagctttaa aatcctttgc tgtttgcatg
     4981 tcaaatgttg taactctgat atatccaata tcatcgccaa gcattgcagg cttaacagtt
     5041 ttaattctta ttttctccct gttaataact acatctgaaa tgtcttgagt tccgtcagaa
     5101 gcagctcttt gaatagttaa tgtaacactt gtacccggct cacctttcat aacctttaca
     5161 gctgcatcaa tttggtctgc cgtatattcg ataccgttaa tacgagtaat tttatcacct
     5221 gttctcagtc ctgccttttc tgccggagta tcctcaatag gtgaaacaat aattatttta
     5281 ttgtcatcac ctgcagttat ataaactcca atgccttcat atgacccttc cgtatcctgc
     5341 attaaagatt caaattcttc cttagtcaaa aactgtgtat atggatcgcc taaagctttt
     5401 aacgttccct gtaacgctcc ttcaagcatt acttcttcat cagcttcata taagaactca
     5461 ttttgtgcta ttttcattat actttcttgt ttggcgtact tttcagatat ttttttcaat
     5521 tggctatatt catcttttga taaaaatacc ttttgtccta tttcaaccaa taacacattc
     5581 ccaagtgtca aggtaagaaa ggctgtaact aatacaaccg caactattgt aagaatatat
     5641 ttcctcttcg ataccatata ctcactcccg ctattaaaaa ttaagctgaa ttaaacgctt
     5701 atatttattt atatgccttc aatattattt tatcctataa aaataataca gccttacata
     5761 caaatgctat tatagcataa atgcaagcga attaatatat ctttttaggg gatattccct
     5821 ttatgagatc cttcgcttac gctcaggatg acaaggcaaa gcttacgttc aggatgccga
     5881 tacagtaaaa aatattttaa cagttatgaa acttacttat ttcttctata catttagcac
     5941 tgaataatgt tgtatccatg cccacagcta tatacaaaaa tccctgctct gcgcgctttc
     6001 ttgcagtttc tccgtttcct gcaaaaatcc ccgctatttt attgtatcgt tttgttactt
     6061 caagaacctt ctcagccgca ctttcaacta attcatgagt tacctgtccc gttactccca
     6121 ttgattgaga catatcgtat ggtcctaaga atataacatc tatttcagga attttacata
     6181 tttcttcgag gttatccaaa catgttttat tttcgcagtg agtgattata agagtttctt
     6241 tatttgcatt atcggtatag ttagaaatat cgcacaagcc gtagtcagcc gaccttggaa
     6301 aagccaaacc tcttttacct acaggctggt acttgcttct ttctataatt tccttagcag
     6361 cttctacgtc attaacctgc gggacctgaa tcccgtgagc tccaatatcg agggtatgaa
     6421 gtattgtgct ctcttgcctg tcaggaattc ttactattgg agttattcct cttacttccg
     6481 ctgctctaat caagtgttga gagctttctg ccctcatagg accgtgttca ttgtctatta
     6541 ttacaaagtc aaaccctgcc aaacctgcta tttcaaccaa atccggagcg ttgcacacaa
     6601 tgaacgttcc aattgccgcc tttccggcat ttaacttttc tttgagctta ttctttatca
     6661 tttttcctcc aatttagatt taattaaatt tcccaatctt tcaattccaa ttcttatttt
     6721 atcgggagga ttggcaccaa agctaagtct catgcaatta ctgcccttgc ccttaccttc
     6781 ggtaaaaaat ccctctccgg caacaaaggc aaccttaact tcaggatttg aaacagattc
     6841 ttttaataat tctgaagtat ttattccacc gggcaattcc acccatgtga ataatcctcc
     6901 gtcaggaaat gtccttttag ttccttcagg gaaaaactta tctatacttt ccagcattgc
     6961 atctcttctt tctctgtgaa tatcgcacag cagcttgtga tgttcggggt agtatcctct
     7021 cttgaaaaac tcggcacaaa ttatctgagg caacagcgag gtatgtgaat ttgaagctga
     7081 ctttgcatct gtcatctttg atattatatc atcatttgca tacacatatc ctattctgct
     7141 tcccggcgaa aatatcttcg aaaaactatt agccagcaca gtgtgaccag tattatcaaa
     7201 tgctttaatc ggcaataaat cttcgccgct gtaccttata tcccggtatg ggtcatcttc
     7261 taatataatt acatcatact tgcttccaag ctctgcaatt ttttgtcttc tctccaagga
     7321 taaggtcctg cctgtaggat tttggaatgt tggaattaca tatactaatt ttggattgta
     7381 tttaataatc ttttcctcca gctcctcggt aaccatccca tcatcatcca tttcaacagc
     7441 aacacatttt gcctcaaaca tactgaatgt ttcaacagaa tgaacaaaag tcggagattc
     7501 caccaaaatt acatcaccgg gctcaatgaa aagctgacac attaagctca ttgcttccaa
     7561 tccgccggtt gtaattataa tattgtcggc atttgcctta accccttttg gagccagcag
     7621 ctcctttact attacatctc ttaaatcctt aacaccaatt acaggtccgt aggacagtat
     7681 ttcataacct cttttctcaa ctgtcataat atcagaggta atttctctta caatatcaac
     7741 aggtaaacat tcctttgcaa ttgccccgcc gccaaaggaa atcacaccgg gatcgttcat
     7801 ggagctgaat aaacctttta taatattggc cggctcctcc atagttttca ttcgttttgc
     7861 atattttgac atattatttc tccttaattc ttattattat ccttaacagt aatcttccgt
     7921 gagcattatt aaatttctta aattatacca tcaaaaatta ctttagccaa ataaaagttc
     7981 aaatatatgt aaaagcaccg gcatgccggt gcttagactg ttgacaaact ctatgtatgt
     8041 catcaattat tccattgatg cggcattatt atagatttta tctatttctc tctttgataa
     8101 taacttttca actgtatatg caataatggc aatcccaaat atattcaata tgaatcttgt
     8161 aatcataaat tttattccca ttgatgatgc ttcgaataat aataatggta tttttgttgt
     8221 agaccaggca ccaatcataa ttaaaacatt tgaaaatttg cttccctttt taagaagcac
     8281 tgcagccacc ggaaaagccg catataatgg acctgctgcg gcagaaccta ataaaaatgc
     8341 tagcaagaca ccgataatcc ctgatttttc acccatgaat ttaatcattg tttctctttt
     8401 tacccaaaca tctaaaagcc ccaacagaat aaaaacaggc ggcagtatgg acaacatttc
     8461 aagggcattg cttttcgtta actgtaaaga ttttagtcca atatccggtt ttacaattaa
     8521 aataatagta ttgataacta agaggattat aaaaaatcta tatcttttaa ttacttttat
     8581 aattttatcc atcacattac aacccccata atgtatgcta ctgctaagga aaaaataaat
     8641 gcctccatat ttctgattat agcagttttt ttgccgaaat attttatttc aagagggatg
     8701 gttactactc caaccatcat aagtgttgaa ataaatgctg ctatctgcat gtagcctgcc
     8761 ccgctcttta ataatgcgga agcaagtgga aatgctataa aaccgggcac aagagttatt
     8821 gagccgatga aagcagcaac caatactcca atccatcctg attcgcttcc taccaacctt
     8881 gaaatttgtt caggtgtaag tatagcaagt gcaattccta taatgacaag tatagtcaaa
     8941 aattgaggta gaatattttc gaatgatttc cacgccttct tcaatgaaag tattgtttta
     9001 tccttatttt taataaatga tacaaccaat gcagtacctg taattaaata caatccaatc
     9061 gtggtcataa tatcctccgt taattggttt ttgtttccta ttatatcact tttcaccatg
     9121 tatgtatgta acaaaagtta ctgatggaat ttattatact actggataaa accaaagccg
     9181 tttatagata aaatatttta aatggtatta tttggtgata gtcttcgatg taggcaaaaa
     9241 gaaaatgaaa gatacagtat caaattaaag aaaacagtat tcgacatttc aacttatcga
     9301 atactgtatc taatattata ttcttatttt attaatatag gaaagacaca ttttatttaa
     9361 ctgtcttaag ccttttattt tttatattac ttaaatttta ttcagaataa ttagcagcat
     9421 tttcacctgc aatttttcca aaaactatat tgactgctgc agggttagct ccatatgttc
     9481 ctgcgcttgc acattctccg gctgcataaa gacccggtat aattgtgcca tctgttttta
     9541 aaacttgtgc atttgtatct gtttttattc ctccaaatgt tccttgtaca tgacacgtca
     9601 tcttgcatgc atagaatggg gcttcatcaa atgttagatt tagcatcttg cgtccaaaat
     9661 cttcgtcaac tcctttttca acgaaacctt tgtatttttc aactgttgca ataagattgt
     9721 ccttattgac tccaatttta tcagcaagtt ctcctattgt aggagcactt tcaaataaac
     9781 ccagttcata atattctctt acttcttcaa tactatctat cattgattga tcaaatatca
     9841 tccagcctgt cccgccttgg tccacaactg ttttcccggc tccataagcg gcttgatcat
     9901 taataaatct ctctccttca ttattcacaa aaattgctcc attatttgga aggcaaccta
     9961 tataataata acgaattccg tctgtataat atgtgaaatt aactcgtata taatccatat
    10021 cccaaagctc tgcgcctaaa gcttcagcca tgaggtgacc gtcacccttt gcgcctatgc
    10081 tgcaggaact attatacccc ttaagttcag gcatatattt tgaaaccatt tcattattag
    10141 ctgcaaaacc gcccgaagca ataataacag ctttagattt tatgacatat tcgcctgcag
    10201 gattttctac ttttacacct gctattttcc cgtcttccat aattatttct gtagcctttg
    10261 tattaacgcg aatatctaca ccagcctttt tcatttctgc cgccaaagca gaagtaataa
    10321 ctaaaccaag aggaccggct tccctgttag atgtaacaga aaacggatta tttgtaacac
    10381 tcaaaggtgc acctatactt tttagccaat ctgcagcctt tccattatta tcagctagta
    10441 tacgaaatgc ttccggatct cccttaggat tagctttcac aaaactctca tagttttgct
    10501 ccgaagtagc agtaatacct aaccctttat gtacacttgt ttctgaagaa ccgaaacttt
    10561 caattgaaaa agcagatgta cctccaacga agcctaattt ttctaccaat ataacttttc
    10621 tgcctgcatt tgctccataa atagctgcag tcattccggc tgacccacca ccaataacaa
    10681 caatgtcagc agttgtgtct tctgcctttt ccgttacagg tattggttct tttaactgtg
    10741 ccggatctgc accggcttct tttacacact gattaacagc ttccataaaa gctacactgc
    10801 ttatagtagc accggatact atatcaacat ccaaggattg attttctaca attattttcg
    10861 gataagtctt tataggcaca tctcctacat tgtatgtttc cctatgactg ataaccctta
    10921 catcatcaat cgtatcatcg ctaaaatcaa tttgtactac tatagggcca tttcttcctt
    10981 tacttatacc tgtataagtt ccggatttaa aaagcgtttc tgtttcattt gcagcatcat
    11041 ttgattcagc tggttccgga ggttgttctt tatcaaccgt ctgattacat ccaactgaag
    11101 aaataacaaa taaacaacat aatataaagc ttattactct tgcatatttc ttcatagttt
    11161 acctcctata aaatactttt agttaattaa aatatagcac ttaaataaca attcatcaat
    11221 attttttatt gcttgtcaga aattttttta tcaataattg ctgaatagag tttattaagc
    11281 ttttattagt aagataaaat tttgttggaa tatcctcact tacttatatt tttaaatatg
    11341 ttataatatt ttcaataaat aaaagaaaag tgatgaaaat acgtttaatt tttgacaata
    11401 atagtcaatt tcaattctat cagatttaca aatactaaca tttggtatat gatattgcat
    11461 agatgataac gttaagcgga gggataagta tgtttagaga aatgcgaaga aaagacagag
    11521 aaatcaaaaa ggatgaagct atcgaaattt taaaaaacaa taattatggt atactgtcaa
    11581 ctattagtca aaatggatat ccatatggcg taccaattag ttatacttat attaatggtt
    11641 caatatattt tcattgtgca gctgaaggac ataaattgga taatattaag aataatgata
    11701 aagtatcttt ttgtgtagtt ggtcaaacta aaaccttacc tgatatattt agcgtaaaat
    11761 atgagagtgt aattgtattc ggaagagtag ttgaagtcta tggtgatgaa aaaaatatgg
    11821 ctcttttaga aatattaaat aagtattcac cggattatat tgaacaagga ctagaataca
    11881 tccaaaacag tagtgaagat acaaaagtaa taagaataaa tgttgagcat atttccggta
    11941 aagcaaggaa gcagacgcaa taatagtata aatcaaaaat gtaat
//
LOCUS       DAOSHP010000055        11799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4588_length_11799_cov_9.222156, whole genome shotgun sequence.
ACCESSION   DAOSHP010000055 DAOSHP010000000
VERSION     DAOSHP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4588_length_11799_cov_9.222156"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLB33_06385"
     CDS             <1..263
                     /locus_tag="PLB33_06385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOG63187.1"
                     /translation="KLAAVEKEVQLLAAEIEKTRRRVNALEYIMIPQLEETIKYIQMK
                     LDENERGNQTRLMKVKDMMVKEAIEEKQQFTKNAIDEYANNK"
     gene            433..1467
                     /locus_tag="PLB33_06390"
     CDS             433..1467
                     /locus_tag="PLB33_06390"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOG63188.1"
                     /translation="MILNELKIGNLTARIPIVQGGMGVGVSLANLAAAVANEGGIGTI
                     SGVQIGFREPDFFTDPIGANLRAIKKEIKKAREKTKGIIAMNFMAAMNNYELYVKKAV
                     EEKIDLIVSGAGLPMALPRLVKGSNTKIVPIVSSEKAAKIIIRSYLKSDRLPDAVVVE
                     GPLAGGHLGFKMEELLENKCMALIDIVKEVKLLLSTFEEKYKVKIPLIAAGGIRTKED
                     IIELKEAGADGFQIASLFVPTVECDAHQNFKSAYINARDDKINIIKSPVGMPGRAIET
                     NFLTQTERIIKKCHKCMPNCNPNEIPYCISEGLINSVEGRDGLIFSGAKLNNVNKMTT
                     VKEVINNLIR"
     gene            2088..3641
                     /locus_tag="PLB33_06395"
     CDS             2088..3641
                     /locus_tag="PLB33_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOG63189.1"
                     /translation="MSGDTLQIFVSMIIYMAVVIGIGLYFAKRANESSENYFLGGRTL
                     SPLVTAMSAEASDMSGWLLMGLPGVAYWYGLSDAAWTAIGLAAGTYLNWLFVAKRLHN
                     YSIAAGNSITLPDFLSNRFKEKNKIIMSISAVFILVFFIVYASSCFVTVGKLFNALFG
                     FKYQYMMILGAAFVLFYTFIGGFLAESASDTLQAFVMIIALVTIFFVGTLAAGGVAQV
                     IENAKEIPGFFEFFGIASPNVTDGVQEVVNGVPQYNEAGEYGLYTILSTLAWGLGYFG
                     MPQVLLRFMAIRDSNELKTSRRIATVWVVISLGAATAIGIIGRAVFPTTLLTYSDSES
                     IFIVLSSNLLPAFLAGFVMAGILAATISSSDSYLLIAASAFSKNIYQHLIKKDATDKQ
                     VMNVSRIILVLICIIGIFIALDENSVIFTIVSFAWAGFGATFGPIIIFSLFWKRTTRE
                     GAIAGMLAGGIMVFVWKLVLRPMGGLFGVYELLPAFIVSCLFIYAVSKITAEPSEEIQ
                     KEFEAVMKM"
     gene            complement(3688..4191)
                     /locus_tag="PLB33_06400"
     CDS             complement(3688..4191)
                     /locus_tag="PLB33_06400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOG63190.1"
                     /translation="MKNIVIIGMPGSGKTTVGMMLSKKLNMEFFDMDDNIVSMENKSI
                     NEMFEISEEYFRNAETRCAKFLNNKTSLIIAAGGGIIKRKENIDYLKQNSIIVFLNRP
                     PEKIIEDIDIKTRPLLREGRDKVFTLYNERLHLYKKYCDIEVLNDKTLDDAVNEIIKR
                     VIPYISS"
     gene            complement(4195..4455)
                     /locus_tag="PLB33_06405"
     CDS             complement(4195..4455)
                     /locus_tag="PLB33_06405"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOG63191.1"
                     /translation="MELDKARTEINKIDKEIVRLLEKRFNIVNEIGNYKRKQGLSIYD
                     ETREKQVVESCISYLENKEYSKFIDDIYFQIMKCSKDIQQEG"
     gene            complement(4425..5534)
                     /gene="aroC"
                     /locus_tag="PLB33_06410"
     CDS             complement(4425..5534)
                     /gene="aroC"
                     /locus_tag="PLB33_06410"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOG63192.1"
                     /translation="MSSMIGNKLKISIFGESHGEAIGVVIDGLPSGYAINLDYINQQM
                     NRRLPGKSNLSTERKEEDEYKILSGFLEGKTTGTPLSAIIYNKDKKYEDYGDRFKLRP
                     GHSDYTGYIKYSSYNDYRGGGHFSGRLTAPLLFAGAIAMQLLESREIFIGSRIKSIYN
                     INDENIENIDAITIKNLKKMSFPIISLEKGALMKTAIIEQKEKGNSLGGIVETFIINI
                     PAGYGEPFFDSVESKISHIVFSIPAIKGIEFGAGFKITELTGKEANDEFKVENNKIIT
                     STNNNGGILGGITNGMPIVFRTAIKPTPSISLSQNTVDISSMENTIINTKGRHDPCIV
                     PRVVPVIEGACALALLDLILERDGEKWSLIKQEQK"
     gene            complement(5512..6771)
                     /gene="aroA"
                     /locus_tag="PLB33_06415"
     CDS             complement(5512..6771)
                     /gene="aroA"
                     /locus_tag="PLB33_06415"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOG63193.1"
                     /translation="MLSKNYKKKSLLIKPSLLKGKITIPPSKSISHRAIICASLSMEE
                     SKISNIVFSDDIRATIEGMKTLGADIKEADDFILVKRSNKILNNAIDCNESGSTLRFL
                     IPLSLVLKDECTFTGKGRLNERPLDIYYEIFRKSNIEYETNAGKLPLKIRGRLKGGFY
                     KIPGNVSSQFITGLFFSLPLLDVGSEIKIIGEPESKNYIKLTLDVMKRYKVNIEKIDE
                     NNYYIKGAQKYKAFDYSIEGDFSQAAFFLAANVLGCKIECLGLNYDSLQGDKEILNIA
                     EKYSSPMEEITIDASQIPDLVPVISVLASLKENCTTKIINAGRLRFKESDRLEATASE
                     MKKLGALIEETKNGLIINGKSSLYGNATVDSHNDHRIAMALAIATIKCDNPIVLEGYE
                     SVKKSYPKFWDDYQSLGGAIYELNDWK"
     gene            6941..7873
                     /gene="arcC"
                     /locus_tag="PLB33_06420"
     CDS             6941..7873
                     /gene="arcC"
                     /locus_tag="PLB33_06420"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOG63194.1"
                     /translation="MSNIIVLALGGNALGNDPQEQKEAVKHTSKSIVDLIEAGNQVIL
                     SHGNGPQVGMINLAMDAASKSEAGTPEMPFPECGAMSQGYIGYHLQNAIQNELKLRGI
                     KKSVATVVTQVVVDKNDPAFQNPTKPVGSFYTKEEAELMTAKGFTFKEDAGRGYRRVV
                     PSPKPIDIVEIDFIKSLVDTGNIVIAAGGGGIPVVEEADGLTGIDAVIDKDFTTAKLA
                     ELVDADSLIILTAVEKVAVKFGKPEQKWLSELTVEEAEKYIADKEFAEGSMLPKVRAA
                     ISFATSKTGRKALITSLEKAAEGLEGKTGTWIIA"
     gene            7987..9822
                     /locus_tag="PLB33_06425"
     CDS             7987..9822
                     /locus_tag="PLB33_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG63195.1"
                     /translation="MKKLNRIILLSVVLALLLTMTANAAVFSDISNHWARSYIERVEK
                     KGLVSGYEDGTFKPDNNVTVLESLVMMSRLYKIDDDIREEIIEKYKPSLKSMKNILYN
                     EWSIDYLALAIELGIVSEQAVKDMFANRNIFNDAKREEVAVLLTMALGLNDEVKNLKT
                     YVLPFNDRDEITASARPYIYLMYEKEIMQGDNEKNINPGDKITRAEISTLLDKAFNYI
                     EDNNILPDLEEYKPTTTVSGMITEVQINKTESYIYVKNYSEVESIVKINGDTKITING
                     RTRELSDLKKDMLVICRINEERLALEIEADSTKDVVRGTIYYVAYVEPASITIYDEDD
                     DKLTFDIGKDVEVLHDGKATELRRLRKNDEVTILLDDDEVIKISSISRIKHYDGVITS
                     IDYNYPIKVTIKTDEDVSKTFIFNKDVEVTRNDDDSSFDQVRVGDEVTITTEYDEMIA
                     INTIAKEAETSGVIREIIIAPQSKIKIADEDGEIKQYSVSSNVKITIGAKNVSIYDLR
                     LGYNVYINTSGDEIVTMEVSEMETAKSFTGKIIYINEDDGLIMMQSVTSTGKELIYLS
                     VTNSTIIYDTSGKTRYFKDLEEGESIVSIAVQQGGEYIATSIMIQ"
     gene            9875..10789
                     /locus_tag="PLB33_06430"
     CDS             9875..10789
                     /locus_tag="PLB33_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63196.1"
                     /translation="MRKFILFITVFTLMLTIGCTQIPKSEETGLGPEGYEGIASTEEI
                     KKENSSLKEELEKIKTEFEVLEENYLRLVKNNESIISKLEETESKLEIISSEDIPEFK
                     SEETDKDSIVSYLNDTSKLIDDSIKGIEIISSGESVVFRTLGYGEEYSQIFIWDEGEN
                     EPVLIDGAVIEKGGSYEWLGKYILIKNKDKNKVLDIENKKITGSFDEPQKLQLLEKTA
                     SILLKDKDNNFVLYDFINNNSKQINLDNNKYTDFDLRNNIIVFSGAYVENDVEYEIRA
                     SIALDKLIEAFDTEGSEEAESEEIEDSV"
     gene            complement(10889..11632)
                     /locus_tag="PLB33_06435"
     CDS             complement(10889..11632)
                     /locus_tag="PLB33_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOG63197.1"
                     /translation="MSSNTEPQKFTSLTVVRAGLFISMSLVLKVMFEVYIPLAGIPAL
                     RINFANIPLMLSGIILGPFTGFLTGAAADIINFIAKPGGPFFPGFTLASGLSGFIPGI
                     IFKYFKRDINYNLVNTTFISLLSAGFILVFMQNGLLTFDNGILYDGKPLNPLYAAGFF
                     VLVAVYLIIPIKLSSATKDLSMNKIVFAISITQLITSIILNTYFLYILYGIGIMITLP
                     ARILTNFFMIPLYSIIIGAILNAVKGRIK"
     regulatory      complement(11698..11791)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
BASE COUNT         3947 a         1836 c         2272 g         3744 t
ORIGIN      
        1 taaaattggc agctgttgaa aaagaagttc aacttcttgc agctgaaatc gaaaaaacca
       61 gaagaagagt taatgcactt gaatatataa tgattcctca gcttgaagaa actatcaagt
      121 acattcaaat gaagcttgac gaaaatgaaa gaggaaatca aaccaggctc atgaaagtta
      181 aagacatgat ggttaaagaa gcaatcgaag aaaaacaaca gtttaccaag aatgctattg
      241 acgaatatgc aaataataaa taaaagttaa ttaattattg tcattctgag tgcactgtac
      301 gaagaatctc aaagcagggg acacgccttt aaaattgaag gaatgtccct gtttttttta
      361 cttgacaaaa gaaacatatg catatatact ttgaatatca aattaattga tttctaaaat
      421 aatggtggaa atatgatatt aaatgaattg aaaataggaa acttaactgc tagaattccc
      481 attgttcagg gaggcatggg cgttggtgta tctttggcaa atttagcggc agcagtcgca
      541 aatgaaggtg gtataggcac tatatccggt gttcagatag gcttcagaga gcctgatttt
      601 tttactgacc ccataggggc aaatttaagg gcaatcaaaa aagaaataaa aaaggcaaga
      661 gaaaagacaa agggtattat tgccatgaat tttatggcag caatgaacaa ttatgaattg
      721 tatgttaaaa aggctgttga agaaaaaata gatttaattg tatcaggcgc aggacttccg
      781 atggcacttc ctagattagt gaaggggagc aatacgaaga tagttccaat agtatcttct
      841 gaaaaagctg caaaaataat aatcagaagt tatctcaaat cagatagact gccggatgcg
      901 gtagtagttg aagggccctt ggcaggtgga catctgggtt ttaagatgga agaattattg
      961 gaaaataaat gcatggcatt aatagatatt gttaaagagg tgaaacttct attaagtaca
     1021 tttgaagaga aatataaagt aaaaatccct ttaattgcag ccggtggaat aaggacaaag
     1081 gaagatatca ttgagcttaa agaagcagga gcagatggtt ttcaaatagc aagcttattt
     1141 gttcccacgg ttgaatgtga tgctcaccaa aattttaagt cagcatatat caatgcaaga
     1201 gatgataaga ttaatattat caaaagtccg gtcggaatgc cgggaagagc tatagaaaca
     1261 aattttctca ctcaaacgga aagaattata aaaaaatgcc acaaatgtat gcctaactgc
     1321 aatcctaatg aaattccata ttgcatatcg gaagggttga taaattccgt agaaggaaga
     1381 gacggtctca ttttttcagg tgcaaaatta aacaatgtaa ataaaatgac tacagtaaaa
     1441 gaagttatta ataatttaat caggtaataa gattaagata atattttaat tcctctaatg
     1501 caacaagggg acagttgcta taatgataac aacttgtctc cttgtcgcat tatgacaagg
     1561 ggacgggggt gttttcatgt atgacaaggg aaagagaatt ttgtaacata gcaggggatg
     1621 gaagcatatt atgaattaaa atacatattt gtttttattg tggggacaag acaatatgta
     1681 aaaacaattg aattaaattt atatgaattt ttaggtaaaa taattcaaaa aatatcttga
     1741 caaagtattt gtattggtgt agtatgtttt ttaatattaa tatttgctgt gaagaggaat
     1801 agtaatgaaa catggactta gagagaaggt tggccggtga aatacctttg tcccaatgtt
     1861 gattgaaccc gcctcagagc gtttgggagt aacaacccaa agggaacacc ccttatttaa
     1921 atgagatgct gtatataatg cagttagagt ggtaccgcgg acatccgcct ctttttgagg
     1981 cggatttttt aataataacc ctgtatttct agggtacggg gacacaaatt ctactgtagg
     2041 gagaaagaaa atcataataa caaaatatta aagaaggagg tttcattatg agtggagata
     2101 cactacagat ttttgtatca atgattattt acatggctgt tgttataggc atcggattat
     2161 attttgcaaa acgtgccaat gagagcagtg aaaattattt tttaggaggt agaacactta
     2221 gcccattggt tacagctatg agtgccgaag catcagatat gagcggttgg cttttaatgg
     2281 gacttccagg tgttgcatac tggtatggct taagtgatgc tgcatggact gcaataggat
     2341 tagcggcagg aacgtattta aactggttgt ttgttgcaaa aaggcttcac aactattcga
     2401 ttgcagcagg taattcaata acacttcctg attttttaag caatcgtttc aaagaaaaga
     2461 ataaaataat tatgtctatt tctgcggtat ttattttagt attcttcatt gtttatgctt
     2521 ccagctgttt tgtaacggtg ggtaaacttt ttaatgcact gtttggattt aaatatcagt
     2581 atatgatgat attaggggct gcctttgtat tattttacac atttatagga ggttttctgg
     2641 cagaaagcgc ctccgataca ttgcaggcat ttgttatgat tattgcttta gtcacaatct
     2701 tttttgtggg aactcttgca gcgggaggag tagcacaagt tatagaaaat gcaaaagaaa
     2761 tccccgggtt ttttgaattt ttcggtatag cctcacctaa tgtaacggat ggtgtgcaag
     2821 aggttgttaa tggagtacct caatataacg aagcaggaga gtatggcctt tacacgattt
     2881 tatcaactct tgcatgggga ttgggatact tcggaatgcc acaggtatta ttgagattca
     2941 tggctatcag agattctaat gagttaaaaa catcaagaag aattgcaacg gtatgggtag
     3001 ttatttcctt aggtgctgcc acagccatag gaataatagg aagagcagta tttcctacta
     3061 cacttttgac atacagtgat tcagaaagta ttttcattgt actttcatca aatttacttc
     3121 ctgcatttct agccggtttt gtaatggcag ggattttagc agcaacaata agttcatctg
     3181 attcatattt attgattgca gcttcggcat tttccaaaaa tatataccag catttaatta
     3241 aaaaggatgc aaccgacaaa caggttatga atgtttcaag gattatcttg gttttgattt
     3301 gtattatagg tatatttata gcccttgatg aaaacagtgt tattttcaca atagtatcct
     3361 ttgcttgggc agggttcggg gctaccttcg gaccgatcat catattctca ttgttctgga
     3421 agagaacaac cagggaaggt gctattgcag gaatgctggc agggggtata atggtatttg
     3481 tttggaagct tgttttaagg cctatgggcg ggctctttgg agtatatgaa cttcttcccg
     3541 catttatagt atcctgtctg tttatttatg cagtatctaa aattactgcg gagccttcgg
     3601 aagaaataca gaaagaattt gaagcggtaa tgaaaatgta ataattttgg tgccaaataa
     3661 ttttggtgcc aggcaccttt ggcaccatta ggatgatata taagggataa ccctttttat
     3721 aatttcattt acggcatcat ccagtgtttt atcatttaaa acttcaatgt cgcaatattt
     3781 cttatacaga tgaagtcttt cattgtatag ggtaaatact ttatctcgcc cttctcttag
     3841 gagagggcgg gtttttatgt cgatatcttc tatgattttt tccggaggtc tgtttaagaa
     3901 tactataatt gaattttgtt tcaaatagtc tatattttct ttccttttta ttattcctcc
     3961 gccggcagct attataagcg atgttttatt gttcaaaaat tttgcacatc tggtttctgc
     4021 atttctgaaa tattcttcgc ttatttcaaa catttcatta atgcttttgt tttccatgga
     4081 tactatatta tcatccatat cgaaaaattc catgtttaat ttttttgaaa gcatcatgcc
     4141 aacagttgtt tttccgctcc ccggcatgcc gattataact atattcttca ttttttatcc
     4201 ttcttgttgt atgtccttgc tgcatttcat tatttgaaag taaatgtcat caatgaactt
     4261 ggaatattcc ttattttcca aataacttat gcaagattca acaacctgct tttctcttgt
     4321 ttcatcatat atggaaagtc cttgttttct tttgtaatta cctatttcat tcactatatt
     4381 aaatcttttt tccaatagtc tgactatttc tttatctatc ttgtttattt ctgttcttgc
     4441 tttatcaagc tccatttttc accatccctt tccaaaatta aatcaagtaa ggcaagagcg
     4501 caagctcctt ctataacggg aacaactcga ggtacaatgc agggatcgtg acggccttta
     4561 gtatttatga tagtattttc catagatgat atatcaaccg tattctgact caaggatatt
     4621 gaaggagtgg gttttattgc ggttctaaat actatgggca ttccgtttgt tattcctccc
     4681 aatatacctc cgttattatt ggtagaagta attattttat tattttctac tttaaattca
     4741 tcattggctt cttttcccgt aagctcagta attttaaaac ctgcaccaaa ttcaattcct
     4801 ttaattgcag gtatggagaa tactatatgt gatattttgc tttcaaccga atcgaaaaac
     4861 ggctccccgt accctgcagg aatatttatg ataaatgttt ccactattcc tcccaaggag
     4921 tttccttttt ctttttgctc aattatggct gttttcataa gggctccttt ttctaatgaa
     4981 atgatgggga aactcatttt ctttagattt tttattgtaa ttgcatcaat attttcgata
     5041 ttttcgtcat ttatattgta aatgcttttt atgcggctgc ctataaatat ttctctgctt
     5101 tccaataatt gcatagcaat tgctcctgca aataaaagcg gtgcggtaag ccttccggaa
     5161 aaatgacctc ctccccggta gtcattatat gatgaatatt ttatgtaacc cgtgtaatcg
     5221 ctgtgaccgg ggcgaagctt aaacctatcg ccgtaatcct catacttctt atctttatta
     5281 taaattattg cggacagcgg ggttcctgta gtttttcctt ctaagaaacc gctgagtatt
     5341 ttatattcat cctcttcttt tctctcggtt gacaaattac tttttccggg aagccgtctg
     5401 ttcatctgtt gatttatgta atccaaattt attgcatatc ctgatggcag tccgtcaatt
     5461 acaacaccta ttgcttctcc gtgtgattcc ccgaatatac ttatttttag tttatttcca
     5521 atcattgagc tcataaatag cacctcctaa gctttgataa tcatcccaaa attttggata
     5581 tgattttttg actgattcat atccttccaa aacaatcgga ttatcgcatt taattgttgc
     5641 aattgccaat gccatggcaa ttctatggtc attatggctg tctacggttg catttccgta
     5701 caaggagctt tttccattaa ttataagtcc attttttgtt tcttctatta aagctcctag
     5761 ctttttcatt tctgaggcag ttgcctcaag cctgtctgat tctttaaatc tcagtcttcc
     5821 tgcatttata attttggttg tgcagttttc tttcaaggat gcaagaaccg atattacggg
     5881 aactaagtcc ggtatttgag aagcatctat cgttatttcc tccataggcg aagaatattt
     5941 ttctgctata tttaatattt ctttgtcacc ctgcagtgaa tcatagttta aacctagaca
     6001 ttcaattttg catcccaata catttgcagc aagaaaaaat gctgcttgtg aaaaatcacc
     6061 ttcaatactg tagtcaaatg ctttatattt ctgagcccct tttatataat aattattctc
     6121 atctattttt tcaatattta ccttgtatct tttcatgaca tccaatgtca gcttgatata
     6181 attctttgat tcgggttcac cgatgatttt aatttctgaa ccaacatcaa gaagaggaag
     6241 ggaaaaaaat aatccggtga taaattgaga acttacattg cccgggattt tgtaaaaacc
     6301 gccttttagt ctgcctctta ttttgagagg gagctttcct gcattcgttt catattcaat
     6361 atttgatttc ctaaaaattt cataatatat atccaagggc ctttcattca aacgtccctt
     6421 tcccgtaaaa gtgcattcat cttttaaaac caaggacaaa ggtataagga acctcagagt
     6481 tgagcctgat tcattgcagt caattgcatt attcaaaatt ttattgctcc ttttaacgag
     6541 aatgaaatca tccgcttcct taatatccgc acccaatgtt ttcattcctt caattgttgc
     6601 tcttatatca tccgaaaaaa ctatattgct gattttgctt tcttccatgg agagagaagc
     6661 acatattatt gctctgtggc ttatactttt tgacggtggg atagtaattt tcccttttaa
     6721 taatgaaggt tttatcaaca aactcttttt tttgtagttc ttgcttaaca taataacacc
     6781 tgctatacgg taatgattaa aatttatcac aatatcatgg tatgtcaata gttttttgaa
     6841 tagcagataa aagtaaattg ctaaattgaa aatatgatgg tacaataaat ttacgtaaat
     6901 agggacattc cttgtccctt gttatataca aggaggaatt atgagcaaca ttatagtatt
     6961 ggcattaggc ggaaatgcat tgggtaatga cccgcaggag caaaaggaag cagttaaaca
     7021 cacttcaaaa tcaatagttg atttaattga agcaggaaat caggtaattt tatctcatgg
     7081 gaacggtccg caagtcggaa tgataaattt agctatggat gcagcttcaa aatcggaagc
     7141 aggcactccg gaaatgcctt ttccggaatg cggtgccatg agtcaaggtt atatcggtta
     7201 tcatttacaa aatgctatac aaaacgaatt gaaactaagg gggataaaaa aatctgttgc
     7261 aacagtagta actcaagttg tggttgataa aaatgaccct gcatttcaaa atcctacaaa
     7321 accagtaggc tctttttaca ctaaggaaga agcagagtta atgacagcta aaggatttac
     7381 atttaaagag gatgcaggca gggggtacag aagagtagta ccttctccaa aaccaataga
     7441 catagtagaa attgatttca ttaaatcatt ggttgataca ggtaatattg ttattgccgc
     7501 agggggcggc ggaattcccg tagtggaaga agctgacgga ttaacgggca ttgatgcagt
     7561 tatcgataag gattttacaa cagcaaagct tgctgaatta gttgatgctg acagcttaat
     7621 aatattaaca gctgtagaaa aggtagctgt gaaatttggc aaaccggaac agaaatggtt
     7681 atctgaactg actgtggaag aagctgaaaa gtatattgca gacaaggaat ttgcagaagg
     7741 ctccatgctt ccaaaggtaa gggctgccat atcctttgcc acatccaaga cgggcagaaa
     7801 ggcattaatt acttcattgg agaaagcggc tgagggtttg gaaggaaaga cgggtacatg
     7861 gataatcgct taagaacagg atgacaaata acgggtgtaa ggaatgttta caaattgtcg
     7921 attcttattg cagtatcaac atctatatga tataatttat taaaaatcgg acatgtggga
     7981 ggtattatga aaaaattaaa tagaattatt ttactttctg ttgtgctggc tttgttgctt
     8041 acaatgactg caaatgcagc tgtctttagt gatatatcaa atcattgggc aagaagctat
     8101 atagagagag ttgaaaaaaa aggacttgtt tccggatatg aagatggtac attcaaaccg
     8161 gacaacaatg taaccgtatt ggaatccctg gttatgatgt cgagattata taaaatcgat
     8221 gatgatatta gagaagaaat aattgaaaaa tacaaacctt ctttaaaatc aatgaaaaat
     8281 attttatata atgaatggtc aatagattat ttggctcttg caattgagct tggtatagta
     8341 agcgagcagg ctgttaaaga tatgtttgca aatagaaata tattcaacga tgcaaaaaga
     8401 gaagaagtgg cagtgctgtt gacaatggct ttgggtttga atgatgaagt gaaaaatttg
     8461 aagacatatg tcctgccttt taatgacaga gatgaaataa ctgcttctgc acgaccatat
     8521 atatacctta tgtacgaaaa agaaataatg cagggagata atgagaagaa tattaacccg
     8581 ggggataaaa ttacaagggc agagatttct accctcttag ataaagcatt taattatata
     8641 gaagacaaca atattcttcc tgatttagaa gaatataaac ctaccacaac agtcagcggt
     8701 atgattaccg aggtacaaat taacaagaca gaatcctaca tatacgtgaa aaattacagc
     8761 gaagttgaaa gtattgttaa aataaatggt gatactaaaa ttacgataaa cgggagaaca
     8821 agagagttat cagatttaaa aaaggacatg cttgttatct gcaggattaa cgaggagaga
     8881 cttgccttag aaatcgaggc agacagcaca aaggatgttg taagaggtac aatttattat
     8941 gtggcatatg ttgagcctgc atcaataact atttatgatg aagatgatga taaacttaca
     9001 tttgatatcg ggaaagatgt tgaagtatta catgacggca aagcaacgga attgagaaga
     9061 cttagaaaga atgatgaagt gaccatttta ttggatgatg atgaggttat taaaattagt
     9121 tccataagcc gtatcaaaca ttatgacgga gttattacat ccattgatta taattatcct
     9181 ataaaggtta caataaaaac agatgaagat gtttctaaaa catttatttt taacaaggat
     9241 gttgaagtaa caagaaatga tgatgactca agctttgacc aggttagagt cggcgatgaa
     9301 gttacaatca ctacagaata tgatgaaatg attgcaatta acactattgc caaagaagct
     9361 gaaacaagcg gagtaatcag ggaaataata attgcacctc aaagcaaaat aaaaattgca
     9421 gacgaagatg gagaaattaa gcagtactcc gtgagcagca atgttaagat tacaatcgga
     9481 gcaaaaaatg tatcgattta cgatttgcgc cttggatata atgtttatat aaacacctcg
     9541 ggagatgaaa ttgttacgat ggaagtttca gaaatggaaa ctgccaagag cttcactggt
     9601 aagattatat atattaatga agatgatgga ttaataatga tgcaaagtgt cacgtcaaca
     9661 gggaaagaac tcatttacct gtcagttacc aacagcacga taatatatga tacttcaggt
     9721 aaaaccaggt atttcaaaga tttggaagaa ggagaaagta ttgtaagtat agctgttcaa
     9781 cagggcggag aatacatagc aacaagtata atgattcagt aaaagcatcg aaagatgctt
     9841 ttttctctat agggatgcga aaggatgtga gagtatgaga aaatttatat tgtttataac
     9901 agtttttacc ctaatgctta caataggctg tactcagata cccaaatctg aagaaacagg
     9961 tttagggccc gaaggatatg aaggtattgc ctctactgaa gaaattaaaa aagaaaatag
    10021 cagcctaaaa gaagaattgg aaaaaattaa aacagaattt gaagtattag aagagaatta
    10081 tttaagacta gtcaaaaaca atgaaagtat tatatctaag ttggaagaaa cagaatcaaa
    10141 attagaaata atcagcagtg aggatattcc tgaatttaaa tcagaagaaa cagacaagga
    10201 cagcattgtt tcatacttga atgacacctc aaaattaatt gatgacagta taaaaggaat
    10261 tgaaataata agttccggtg aaagtgtagt atttagaacc ttgggatacg gagaagagta
    10321 cagtcaaata ttcatttggg atgaagggga aaatgagccg gtgttgattg acggagcagt
    10381 aattgaaaag ggaggctcct atgaatggct cggcaagtat attttaatta agaataagga
    10441 taagaacaag gtattagata ttgaaaacaa gaaaataaca ggctccttcg atgagccgca
    10501 aaaattgcag ttattggaga aaactgcttc aatattgctt aaagacaagg ataataactt
    10561 tgttttgtat gattttataa ataacaatag taaacaaatt aatttagaca ataataaata
    10621 tactgatttt gatttacgaa acaatataat cgtattttca ggtgcttatg ttgaaaatga
    10681 tgtggaatat gaaataaggg catccattgc tttggataaa ttaattgaag catttgatac
    10741 ggaaggaagc gaagaagcag aatcagaaga aatagaagat agtgtttaat gtattaaggt
    10801 acaaagataa gggcagattt attacctgcc ctttaatttt tcaaataata agctttcgtc
    10861 attgctatga ataacctgtg aaagattttt atttaatcct tccctttaca gcatttaata
    10921 tagcaccgat gattattgaa taaagaggta tcataaagaa gtttgtcaat attctcgccg
    10981 gcagggtaat cattatacct atgccatata aaatatataa aaagtaagtg ttcaaaatta
    11041 ttgatgtaat cagctgagtt atactgattg caaaaactat tttattcatg ctcaggtctt
    11101 ttgttgcaga tgacaactta attggaatta taagatatac agctacaagc acaaagaacc
    11161 ctgctgcata taaaggattt aaaggcttcc cgtcataaag tataccattg tcaaatgtca
    11221 ataatccatt ttgcataaaa accaaaataa aaccggcaga caataaagaa ataaaagttg
    11281 tattgactaa attataattt atatcccttt taaaatattt aaaaataatt ccgggaatga
    11341 aaccgcttaa tccgctggct aaggtaaacc cggggaaaaa gggccctccg ggctttgcga
    11401 taaagttgat tatatctgca gcggctccgg ttaaaaatcc cgtaaacggt cctaatatta
    11461 tgcctgaaag catcaacgga atgttagcga agtttatcct aagtgccggt attcctgcca
    11521 aagggatata tacttcaaac attactttta acacaaggct cattgatata aaaagccctg
    11581 ctcttacaac agttaatgat gtgaattttt gtggctcagt attactactc atattttcct
    11641 ccttattgag caaagcaata cctccgcaca atataggagc aacatatgtt tctctaatac
    11701 agcggaagcg ataagaaacc gcagacataa gtctttcgtt tgagggcgac atcccatccc
    11761 tcaacactta acgcatctta tctactctga attttttat
//
LOCUS       DAOSHP010000056        11724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4622_length_11724_cov_8.025281, whole genome shotgun sequence.
ACCESSION   DAOSHP010000056 DAOSHP010000000
VERSION     DAOSHP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4622_length_11724_cov_8.025281"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PLB33_06440"
     CDS             complement(<1..635)
                     /locus_tag="PLB33_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014468242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOG63198.1"
                     /translation="MKTQKFDVTGMTCSACSARIEKNINKTEGILEANVNLLTNSMTV
                     KYDESVLSEEDIIKVVEDTGYGASTVMQKNKDIKKDAKDDKSEIREMKKRVIISLIFA
                     IPLFYISMGHMLNWPLPKIFHGTENALIFAFTQFLLCLPVIIVNNKYYKVGFKTLFKG
                     SPNMDSLIAIGTSAAAAYGVYAIYKIGYGLGHMDMDMVRQFSMDLYFESAAV"
     gene            complement(649..912)
                     /locus_tag="PLB33_06445"
     CDS             complement(649..912)
                     /locus_tag="PLB33_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOG63199.1"
                     /translation="MKADKDKINRLLKTAAGQIEGISKMVDEDRYCVDISNQILAVQS
                     ILKKANNEILKAHLHMCVKEAFNEGNEDEKIDEIISIINKVSK"
     gene            1096..2628
                     /locus_tag="PLB33_06450"
     CDS             1096..2628
                     /locus_tag="PLB33_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOG63200.1"
                     /translation="MRKEKIIIGTLILTIATTSVRFVGMFFRIYLANTIGSEGMGLYQ
                     LILSFFFLMVTLATSGIRVAISKLISEQIALGNYLNAKKVLHQSIGISIFTGFAAGAV
                     LYYYADYIGSNILNDDRTVLALLYLAPSLPIMAISNCYKGYFYALGKVTQPAIIQMTE
                     QFVRIFLIFYLMGAFLPKGLEYGCAAVAIGMTAEEVFALFLVWFFYIFDKKPRLTKTQ
                     KKADNMVFKILQISVPVSATSYINSILRTVENTLIPARLLLYGLSSEIAISLYGMIKG
                     MVLPLLFFPSSILTSLSSLLIPAVAGDNALANEKSVSRTLSQVIHFTTISGVLVVAVF
                     VSFPNEIAMAVYNNRQVGLMIKLISYVCPFMYLNMVISSMLNALGEQVSSFIVNIIES
                     VLKIFIIYVFVPVYGFNAYLFALFMTTILNTILYLFRLLQVSSIVFDISDWIFKPIVA
                     AVIAGILSRYSYFLFVQNSRHIISLISSICILCLLYLMGLIAFKSVRLESINDLKKYV
                     LK"
     gene            2755..3294
                     /locus_tag="PLB33_06455"
     CDS             2755..3294
                     /locus_tag="PLB33_06455"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOG63201.1"
                     /translation="MHYNTMDSLLFFLIYGFLGFLLETTFRSIAGRKVYISRGFLTNG
                     FCPLYGICALLIIEGFILCELALSNPLTAMIVATAGSIFLVTLIEYIVGRMLDRVFHF
                     KLWDYSAYRLNLHSYICLEFSLLWGIMALLLVYFMHPVMEVLVFAIPYNTRAVAVVLF
                     LSMLIINASYQIRKRVYGG"
     gene            complement(3323..4942)
                     /locus_tag="PLB33_06460"
     CDS             complement(3323..4942)
                     /locus_tag="PLB33_06460"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOG63202.1"
                     /translation="MKYNLIFGHKTPDTDSVCSAIALSHLKNKLNEPSKAYILGDINK
                     ETAFVLKYFNVSVPEILDNVKIQIKDLDFERVKPFTKNKSVHFAYFHMNENKLRTLPI
                     VDEENNLCGLITMKDIAMSLINTDQHYLCTSYDNIIETLLGKSILKFDEIIKGYVIVA
                     AFEEKTLRKSDIVNENSIVITGDRYDVIKYAIESKVRLIIVTGDLKVTDELIEMAIKN
                     KVNIIGTAYHTYYTAKNISLAKYVDDIMKKNDIMMFSEEDYLNDCKEDIEQSKHSKFP
                     IISKDKKYLGLLSRSHLINPKKKKVILVDHNEMNQSADGIEEAEILEVIDHHKIGDIK
                     TSIPISFRNTPVGSTSTIIFQMYRENNVELSKDIAGLILSGIISDTLLFKSPTTTGRD
                     KYAVNELLKIVDLDLHEYAMDMFRAGTSLEGKTLEEIIYQDFKKFNLVYKNVGISQVF
                     TLDINEIMNRKEEFVKLIDDITSDKYYLIIMAVTDIVNEGSYIFYTSSKEKLVRMIFE
                     EDVHQGIYIDKCISRKKQIVPKVINALNQLK"
     gene            complement(5032..6018)
                     /locus_tag="PLB33_06465"
     CDS             complement(5032..6018)
                     /locus_tag="PLB33_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63203.1"
                     /translation="MAMNKQIIENIMTDYNKKRLRAAHEADKKKEKLYAALPRLSEID
                     KQMKLLSISLSKSFLSHPEDLDVQVKKLRKEIEELKKERQSIYEENNIPKDYLEIEYE
                     CKKCSDTGYTTDGKKCSCFKRQIINNLFQMSNMVNMLQKENFNTFDINVFSNESYKNE
                     KLTPRQNMYYVLEISQDFCSNFHETNMNLLFFGGTGLGKTFMCNCIANELLNKEISVL
                     YQTSFSLFETIENHKFNKLNETEENLMNYNMIFDCDLLIIDDLGTEVSNSFTNAELFN
                     IINERMITDKRTIISTNLSLEQLRDTYSDRITSRIFNNFVPLKFYGKDLRFK"
     gene            complement(6005..7066)
                     /locus_tag="PLB33_06470"
     CDS             complement(6005..7066)
                     /locus_tag="PLB33_06470"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOG63204.1"
                     /translation="MYFIFQEMQIDLGDTPIENIFINDYMPQADGTYVKVYLMGYKYA
                     KDRLDFNNETISKNLNLPLSDVLNAWNYWENEGIIIKHETDDEYNYIVEFVNLKQFYV
                     DNVYKHIRNVNDKNSYVDNDKLISTARNPEIKTMMEEIEDMLGRPLKVSEKQKIITWL
                     NKYKTKPYMMTQAFSYSVNNKKIKRFNYIESVVSAWYDAGVVDLDTMVEYLEKRNDRF
                     SAYSRISKALGFNRTLTEAEMRTIDRWIDEWDFSMEMILKCLDNSTKINNPNLNYFDK
                     ILSDWYKNGFKTIEDLKNDKKPEKTKKETSKCKNKFHNFIQSEDISGDDLEKIAREKF
                     KKKLNKLGLAIPEENNGNE"
     gene            7312..8790
                     /locus_tag="PLB33_06475"
     CDS             7312..8790
                     /locus_tag="PLB33_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG63205.1"
                     /translation="MKIKDLTFNIFNKNLNSKIFLQLDEKKDRQPLKLTKRTKGTIAL
                     LLVTSMILSVVYASDYLKTHENNVFATDSFTIVSGENELCRVRDPELLNSVLKKIDKD
                     LENRLHHEIKIESKYSLKPSKAKDKEIATEDELYDLINSNLIYSIMAYEINVNGERIG
                     IVNSEYEAKSIIEEVKHHFSKDYDSESLLEVTTVEDIEIKQVKATNAEIQEKEKLVDY
                     IIKGTDEEKKYTVEKGDSYWSIAEYFEMELEELLVANSASEDDIIQIGDELNLIVPKP
                     FINVQVKRKVVQEEKMPYDTEYVKVSYMYNDEEIVTKAGKYGVSEIEAIVTEQNGIQI
                     AKEVLSEKVITEPVTRIVTTGTQDPPPKQGTGYFINPLPGSMITSRFGNRAGGFHRGQ
                     DMAKAAGSAIKAADGGTVLFAGWSGSYGYMVDIDHGGGFTTRYAHCSDIYVSAGEKVY
                     QGKIIAAVGSTGVSSGPHLHFEVRKYGSLVNPASYIGVQYRE"
     gene            8968..9456
                     /locus_tag="PLB33_06480"
     CDS             8968..9456
                     /locus_tag="PLB33_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG63206.1"
                     /translation="MLYLDNRFMEVALPIIEHEEYQQMRYIKHHDESVFEHSLKVAFY
                     SYQFAYKQNLDWESTIRGALLHDFFLYKFKKCFSLRIITDSIKHAIIHPKIAFDNAVK
                     FFDLNKKEENIIKGHMFPFGFPKSKEAWIVSFVDKYIAVFEYSSNFKKMMHKKQPAYQ
                     AE"
     gene            9668..10198
                     /gene="raiA"
                     /locus_tag="PLB33_06485"
     CDS             9668..10198
                     /gene="raiA"
                     /locus_tag="PLB33_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG63207.1"
                     /translation="MKISIYSKNLQLTDALREASIKKLRRLDKFFQQDIEAKVVLSIE
                     KKIHKVEVTIPFNGRIVRVEEYSDDMYNALDEAVESLEQQIRKHKTKLQNKRHSSQSI
                     KFENIEPLDYEEEEEFKVVKTKRFAIKPMNIEEAILQMDLLKHNFFVFLNADTEEVNV
                     VYKRKDGNYGLIEPEL"
     gene            10833..11216
                     /locus_tag="PLB33_06490"
     CDS             10833..11216
                     /locus_tag="PLB33_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63208.1"
                     /translation="MLESIIRIAVPILTLIFGALSAYFRARDKLRDSSIKYIAEAEEL
                     YKDSTKAGKEKFSWVVDALYDLIPAPLKMFITKKCIEKIVQSSFDAIEDYAKMQFEIA
                     IEKYLKHLSDGKSGDFIDFIKSDCE"
     gene            11213..>11724
                     /locus_tag="PLB33_06495"
     CDS             11213..>11724
                     /locus_tag="PLB33_06495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOG63209.1"
                     /translation="MIRLKHLPLDDISISSPYGARSITVNGRYYWWHNGLDLKAQLNT
                     PVYAVADGEVMAARYDNSYGYYIAIDHGKFGTLYAHLSHYKVTEGSIVKSGEIIGHTG
                     NTGDTTGPHLHFEIRLGSYANFWERAHCDRSVFMNTIDPMLFIEDFFNKKDNLSVEEA
                     AKIVQSAAGL"
BASE COUNT         3863 a         1938 c         1890 g         4033 t
ORIGIN      
        1 acggctgcag attcaaaata taaatccatt gaaaactgcc taaccatgtc catatccata
       61 tggccaaggc catagccgat tttatatatg gcataaactc cgtaagccgc agctgctgat
      121 gtacctatgg caatcaatga atccatgttg ggagaaccct taaacaaagt tttaaaacca
      181 accttgtaat atttattgtt aacaataatt accggaaggc agagcaaaaa ttgtgtaaat
      241 gcaaaaatca aagcattttc agttccgtga aatattttgg gcaaaggcca gttaagcatg
      301 tgccccatgg aaatgtaaaa taagggtata gcaaatatca atgatataat cactcttttt
      361 ttcatttctc tgatttcgga tttatcatct tttgcatcct ttttaatgtc tttgtttttt
      421 tgcatgacag ttgaagcacc gtatcccgta tcttcaacaa ctttaattat atcctcttcg
      481 cttaatacgg attcatcata ctttaccgtc atgctgttag tgagcaaatt tacatttgct
      541 tctaaaatgc cctcggtttt attaatattt ttttctattc ttgcagaaca tgcagagcaa
      601 gtcatgccgg ttacgtcaaa tttttgtgtt ttcattagtg tcactccttt atttagaaac
      661 cttgttgatt attgaaataa tttcatcaat tttttcatct tcatttcctt cattaaaagc
      721 ttcttttacg cacatgtgca aatgcgcctt caaaatttca ttgtttgctt ttttaagtat
      781 agactgaacc gccaatattt gatttgaaat atccacgcaa tacctgtcct catcaaccat
      841 tttggaaatg ccttcaattt gtccggctgc agtttttaac aatctattaa ttttatcttt
      901 atcggctttc attatttcac ctctttggcc ttaattccct acccccctgg ggggtatgtt
      961 tttataataa accacctttt ttcatatgtc aagagaaaaa tataataatt acatttttaa
     1021 ttataccctc aatggcttaa aataaatcga aattttcagc atatagtata ttagttgaaa
     1081 attttgaggt gaggtatgag aaaggaaaaa attattattg gtacattaat attaacaata
     1141 gctactactt ctgtccgctt tgtgggaatg ttcttcagaa tatatttagc caataccata
     1201 ggctcggaag gaatgggttt gtatcaatta attctttcct ttttcttcct tatggtcacc
     1261 cttgcaactt cgggaatcag ggtagctatt tcaaaactga tttcagaaca gattgctttg
     1321 ggaaattatt tgaatgctaa aaaagttctc caccaatcca taggtatttc tatttttaca
     1381 ggttttgcgg ccggtgctgt actgtattac tatgctgatt atataggctc taatatattg
     1441 aatgatgaca ggacggtatt ggctttgctg tacttggctc caagcctccc cataatggca
     1501 atttcaaact gctacaaagg ttatttttat gccttgggta aagtcactca accggcaata
     1561 atacagatga ccgagcaatt tgtaagaata tttttaatat tctatttgat gggggctttt
     1621 ctccccaaag gtttggaata cggatgtgct gcagttgcca tcggaatgac tgcagaagaa
     1681 gtatttgctt tatttttggt ttggtttttc tatatattcg ataaaaaacc tcgcttgacc
     1741 aagacacaga aaaaggcgga taatatggtt tttaaaatcc tgcaaatttc tgttcccgtt
     1801 tctgccactt cttatattaa ctcaatatta agaacagttg aaaatacatt aattccggca
     1861 agacttttac tgtacggttt atcttccgaa attgccataa gcctttatgg tatgattaag
     1921 ggaatggtgc ttccattatt gttctttcct tcttcaatat tgacatctct ttcatccttg
     1981 ttaattcctg cagtagcggg agataatgcg cttgccaatg aaaaaagtgt ttcgagaaca
     2041 ctgtctcagg taattcactt tacaactatt tctggagtat tggtggtagc ggtatttgta
     2101 tcattcccca atgaaatagc catggctgtt tacaacaacc gccaagtggg tctcatgata
     2161 aaattaatat catatgtgtg tcctttcatg tatttaaaca tggttatatc atccatgcta
     2221 aatgctcttg gagagcaggt aagctctttt attgtaaata ttattgaatc tgtgttgaaa
     2281 atttttatca tatacgtatt tgtccctgtt tacggattca atgcatactt gtttgcattg
     2341 tttatgacta caatacttaa tacgatttta tacctgttta gattattgca ggtttcaagc
     2401 attgtttttg atatttccga ctggattttt aaacccattg ttgctgcagt aattgccgga
     2461 atattatcta gatattcata ttttttgttc gtgcaaaatt cccgtcatat tatttcctta
     2521 atatcgtcaa tatgcatatt atgcctctta tacttaatgg gactgattgc ttttaaaagt
     2581 gtacgacttg aatccataaa tgatttaaaa aaatatgttt taaaatgatg acaagacccc
     2641 cgtccccctt gtcataaaac tggaaaattc aataattcaa aacataaatt ggcattctta
     2701 taatagcata tttcttaaat tactccaaag aatacatttc aaggagtgga aaaaatgcat
     2761 tataatacta tggattcatt gttgtttttt ttaatatacg gattcctggg attcttgctt
     2821 gaaacaacct ttagaagtat tgcgggcaga aaggtttata taagcagagg atttcttact
     2881 aatggatttt gtcccttata tggaatctgc gcattattga taattgaggg ctttatattg
     2941 tgcgaattag cgctaagcaa ccccttaacg gcaatgatag ttgcgacagc gggaagtatt
     3001 tttttggtca ccctaattga atatatagtt ggtagaatgc tcgacagggt atttcatttt
     3061 aaattatggg attacagtgc atacaggtta aacctgcatt catatatatg ccttgaattt
     3121 tcattgcttt ggggaattat ggcacttttg ctggtatatt ttatgcatcc cgtgatggag
     3181 gtactggtgt ttgcaatacc atataatact agggcagttg ctgtagtttt atttttatca
     3241 atgttaatta ttaatgcatc ctaccaaata agaaagaggg tctatggagg atgagaccct
     3301 ttaaggaatt aatccggtga aattatttta actggtttag ggcgtttatt actttgggaa
     3361 ctatttgttt ttttcttgag atgcatttat ctatatagat accttggtgt acatcttctt
     3421 caaaaatcat tcttaccaac ttttcctttg atgaagtata aaaaatataa gagccctcgt
     3481 ttacaatatc cgttactgcc attattatta agtagtattt atcagaggta atatcgtcga
     3541 taagctttac aaactcttcc ttcctgttca ttatttcatt aatatccaag gtgaatacct
     3601 gggatatacc cacattctta taaaccaagt taaatttttt aaaatcctga taaattattt
     3661 cttccaatgt ttttccttcc aaggatgttc ctgccctaaa catatccatt gcatattcat
     3721 gtaaatctaa atcaacaatt tttagcagct cgtttactgc atatttgtct cttccggttg
     3781 tggtaggaga cttaaaaagc agcgtatcgg agattattcc cgacagtatg agcccggcta
     3841 tatcctttga taattctaca ttgttctccc tgtacatttg aaaaattatg gtgcttgtgc
     3901 ttccaacagg tgtatttctg aaactgatcg gaatagaagt cttaatatcc ccgattttat
     3961 ggtggtctat aacttccaat atttcggctt cttcaatccc atcggcactt tgattcattt
     4021 cattatggtc aaccaaaatt accttctttt tcttcggatt gattaaatgg cttctgctaa
     4081 gaagccccag atatttttta tcctttgata ttatggggaa ttttgaatgc ttggactgct
     4141 caatatcctc cttgcagtca ttaagataat cttcctctga aaacatcata atatcattct
     4201 tcttcataat atcatccaca tatttagcaa gagatatatt ctttgcggta taatatgtat
     4261 gataagcagt gcctattata ttaaccttat ttttaattgc catttcaata agctcatcag
     4321 taaccttcaa atcccccgtg actattatga gtcttacttt tgattcaatg gcatacttta
     4381 tcacatcata tctgtcgcct gtgattacaa ttgaattttc atttacaatg tctgatttgc
     4441 gaagtgtttt ttcctcaaag gcagcaacaa ttacataacc tttgattatt tcatcaaatt
     4501 tcaaaatact ttttccaagg agtgtttcaa ttatattatc ataagatgtg catagataat
     4561 gctggtctgt atttatcaaa ctcatggcaa tatccttcat tgttattagc ccgcacaaat
     4621 tattttcttc atcaactata ggaagagtcc taagtttgtt ttcattcatg tgaaaataag
     4681 cgaagtgtac tgatttattc ttggtaaagg gcttaactct ttcgaaatct aaatccttta
     4741 tttgtatttt tacattatcc agaatttccg ggacacttac attgaaatat tttaaaacaa
     4801 aggctgtttc cttgttaata tcacctaata tatatgcttt tgaaggttca ttgagcttat
     4861 tttttaagtg actaagagcg atagcagaac atacgctgtc agtgtccggg gttttatgcc
     4921 cgaaaattaa attatatttc ataaaatacc tcatttacgt aatattagtg agtttcagag
     4981 tgaaaggaat cgtcccccaa atatcgagga ctaaaagaac cgtacccgta actatttgaa
     5041 gcgaaggtct tttccgtaga atttaagagg aacaaaatta ttgaatatac gggatgtaat
     5101 tctgtcagaa taagtatccc taagctgctc caaggataaa ttagttgaaa ttatcgttct
     5161 tttatcggta atcattcttt catttataat attaaataac tccgcattgg taaatgaatt
     5221 gctcacctct gttccaaggt catcaataat aagcaaatca caatcaaaaa tcatattgta
     5281 attcatcagg ttttcttcag tttcattcag cttgttgaac ttatgatttt caattgtttc
     5341 aaacaaggaa aaagaagttt ggtacaaaac agaaatttct ttattcaaaa gttcatttgc
     5401 aatacagttg cacataaatg tcttgcctaa gccggttcct ccgaaaaata aaagattcat
     5461 gtttgtttca tgaaagttgc tgcaaaaatc ctgacttatt tccaaaacat aatacatgtt
     5521 ctgccttggt gtaagctttt cgtttttata gctttcattg ctgaaaacat tgatgtcaaa
     5581 tgtattaaag ttctcttttt gaagcatatt aaccatattt gacatttgaa acagattatt
     5641 tattatttgt cgtttaaaac agctgcactt tttcccgtca gtagtataac cggtatcact
     5701 gcactttttg cattcatatt caatttccaa ataatccttt ggaatattgt tctcttcgta
     5761 aatagactgc ctttcttttt taagctcttc tatttccttc cttaattttt taacctgtac
     5821 atccaaatct tcaggatggg ataaaaaact cttggataaa ctgatactta ataatttcat
     5881 ttgtttatca atttcagaca ggcgcggaag agcagcatat agtttttcct tttttttatc
     5941 tgcttcatgg gcagccctta atcttttttt attatagtct gtcataatat tttcaattat
     6001 ttgtttattc attgccattg ttttcctccg gaattgctaa tcctaattta ttaagcttct
     6061 ttttaaattt ttctcttgcg attttctcta aatcgtctcc agaaatatcc tcgctttgaa
     6121 taaaattatg aaacttgttt ttacacttgg aagtttcctt ttttgttttt tcaggttttt
     6181 tgtcattttt taagtcttct atagttttaa aaccgttttt gtaccagtca ctcagaattt
     6241 tgtcgaaata atttaaatta ggattattaa tttttgttga attgtctaaa cattttaaaa
     6301 tcatttccat agagaaatcc cattcatcaa tccatctatc gattgttctc atttcggctt
     6361 cggtaagtgt cctgttaaaa cccagggctt tgcttatcct tgaataagct gaaaacctat
     6421 cattacgttt ttcaagatac tcaaccattg tgtccaaatc taccactccg gcatcatacc
     6481 atgctgaaac aacagattct atgtaattga atctcttaat ctttttattg ttaacagaat
     6541 aactgaaagc ttgagtcatc atatatggtt tagttttata tttgttgagc caagtgatta
     6601 ttttttgttt ttcactgact tttaaaggtc tccctaacat atcttctatt tcttccatca
     6661 ttgtttttat ttcaggattt ctggcagtag aaattaactt atcattatcc acataactat
     6721 ttttatcgtt aacatttcga atatgtttgt acacattatc gacatagaat tgtttaagat
     6781 ttacgaattc aactatatag ttatattcat catctgtttc atgtttaatg attatacctt
     6841 cattttccca ataattccat gcattcaaaa catcgctcaa aggcaggttt aaattctttg
     6901 aaatagtttc attgttaaaa tccagcctgt ccttagcgta tttgtatccc atcagatata
     6961 cttttacata agttccgtca gcttgaggca tgtagtcatt aataaaaata ttctctatag
     7021 gggtatctcc caggtctatt tgcatttctt gaaaaataaa atacatgaat ttctcctttt
     7081 tctacataat ttgccataaa gattgtaata taattgtaac aatcttgtta taataatgtg
     7141 aaaacatttt ctcctaaaaa tattgacata atgacatgta atagaatata atgtcttaaa
     7201 gatatattaa agacatttta attaacttta ttatatcatt tttttatata aaagaaaaat
     7261 gttatttttc ttgtgatagt agtagaaaat tatagctagg aagtagaact tatgaaaatc
     7321 aaagatctaa catttaatat ttttaataaa aatttaaact ccaaaatatt cctgcaatta
     7381 gatgaaaaaa aagatagaca acccctcaaa cttaccaaac gaaccaaagg aacaatcgca
     7441 ttattactcg taacaagcat gatattaagc gtagtctacg cttcagatta tttaaaaacc
     7501 cacgaaaaca atgtatttgc aacagacagc tttactatag tatccggaga aaatgagctg
     7561 tgccgggtaa gagacccgga actgctgaat tcagtattaa agaaaataga taaagattta
     7621 gaaaatagat tacatcatga aataaaaata gaaagtaaat acagcttaaa accctcaaaa
     7681 gcaaaagaca aagaaattgc aacggaagat gagctttacg atttaattaa ttcaaattta
     7741 atttattcca ttatggcata cgaaataaat gttaatggag aaagaatagg gattgtaaat
     7801 tccgaatatg aagcaaaaag tataattgaa gaagttaagc atcatttttc caaagactat
     7861 gacagcgagt cactgctgga agttactacg gttgaagaca tagaaataaa gcaggtaaaa
     7921 gcaactaatg cagaaattca agaaaaagaa aaactggtag attacataat taaaggaacc
     7981 gacgaagaga aaaaatatac agtagaaaaa ggcgattcat actggtccat agccgaatat
     8041 ttcgaaatgg aattagaaga gttgctagta gcaaactcgg catctgaaga tgatattatt
     8101 caaataggag acgaattgaa tttaattgta ccaaaacctt ttataaatgt acaagtaaag
     8161 cgaaaagttg tacaggaaga aaaaatgcct tatgatacag aatacgtaaa agtttcatac
     8221 atgtacaatg atgaagaaat tgttacaaaa gccggtaagt acggtgtttc ggaaatcgaa
     8281 gcaattgtga ccgagcaaaa cggtattcaa atagcaaaag aagtattgtc ggaaaaagtg
     8341 ataacagagc ctgtaactag aatagtaaca acaggaacac aagatccgcc gccgaaacaa
     8401 ggaacaggat attttataaa tcctcttccg ggctcaatga taacttcgcg attcggcaac
     8461 agagccggag gcttccatag aggccaggat atggcaaagg cggcaggttc agcaataaag
     8521 gctgctgacg gaggaacagt attatttgca ggctggtcgg gaagctacgg atacatggtt
     8581 gacatagacc atggaggcgg ttttaccaca agatatgcac actgcagcga tatttacgta
     8641 tctgcgggag aaaaggtgta ccaaggcaaa ataatagcag ccgtaggctc tacaggagta
     8701 tcatcaggac ctcatctgca ctttgaagta agaaaatacg gctctctggt gaatcctgca
     8761 tcatatatag gggtacaata cagagaataa catttataaa ctcataaata cagacaagcc
     8821 gatgaaaaat catcggcttt tatatttagt cttaccttca tccaattttt gcatgtttta
     8881 ttaaaaattt ttattgcaat tttttgagtt tcattataag atgagaaagt attatgcaat
     8941 gcgcaactac taataaagga cggacagatg ttatatttag ataataggtt tatggaagta
     9001 gcacttccaa ttattgagca tgaagaatat cagcaaatga gatatattaa gcaccatgat
     9061 gaaagtgtat ttgaacattc attaaaagta gcattttatt catatcaatt tgcatataaa
     9121 caaaatttag actgggaatc aacaataaga ggggccttgc tccatgactt ttttctatat
     9181 aagtttaaaa aatgttttag tttgagaata ataacagatt caataaaaca tgcaataatt
     9241 catccaaaaa ttgcttttga caatgcagtt aaattttttg atttaaacaa aaaagaagaa
     9301 aatatcataa agggacatat gtttcctttt ggttttccga aatcaaaaga agcatggatt
     9361 gtaagctttg tggataagta tattgctgta tttgaatact cttctaattt taagaaaatg
     9421 atgcataaga aacaaccggc ataccaagcc gaatagtatt atggctataa ataattccat
     9481 agtgtcattc tgagggcatt gcccgaagaa tctcataaga gccttcgctt acgctcagga
     9541 tgacagtata taattagaca ccccaacgga tgtctttttt ttattttgac aaatattttg
     9601 tttatttttc tcataaatgg gtaaaattta aatatatata aataaatact tgaaaggggc
     9661 tgactaaatg aaaatatcta tctacagcaa aaatttgcaa cttaccgatg cattaagaga
     9721 ggcatcaatc aaaaagctaa ggaggttaga taaatttttt caacaagaca ttgaggcaaa
     9781 agttgtttta agtattgaaa agaaaataca taaagtagag gtcacaattc ccttcaatgg
     9841 aagaattgta cgtgttgaag aatattcgga cgacatgtac aatgcattag atgaagcagt
     9901 tgaatcttta gagcagcaaa taagaaaaca taaaacgaaa ctacagaata aaaggcactc
     9961 aagtcaatca atcaagtttg aaaacatcga gcccttagac tacgaagaag aggaagaatt
    10021 taaggtagta aaaaccaagc gctttgcaat taagcccatg aacatagagg aagcaatact
    10081 gcaaatggat ttattaaaac acaatttctt tgttttcctt aatgcagata ccgaagaagt
    10141 aaacgtagta tataaaagga aagatggaaa ttacggttta attgagccgg aattgtaaaa
    10201 cctaacttta aatatagata tcaaaaagac gcagctcgcg tcttttttta cttttacctg
    10261 catccttaac gtaccataat acttaaacac ttaatagtta tataagaaat acgtttgcat
    10321 gattgtcgaa taatgtataa atttagcact tatgctttac tcataattat accttgttaa
    10381 taatttaagt gtataaatta agttgtccac aggcgtggat aaattgttta atttctaata
    10441 atgcttaata ttgaaatttc aacaaaaaat atttttgtat ttttgtaaaa aaacacttga
    10501 caaatatttc ccttaaagtc cccgaattta agaattgtta acaatttaat ccacagctgt
    10561 ggataagttg aaaacatata gaacatatct tctttttttt tgagattgtt aaatatattt
    10621 aaccggtttt aatcttattt aattagttga acatccaatg gaaaagtctg gaatgttaat
    10681 tttctatttt tttgaataat taatagttaa gtttacttgt aattttataa aactaataat
    10741 tattatacac atttttcaca actgccttaa ttacaattca ttaagaacaa atattaatct
    10801 taatgaatat aatgcctaag ggatgtgata acatgttgga atcaattata agaattgctg
    10861 ttccgatttt aaccttaatc tttggagcat tatcagcata ttttagagct cgtgacaaac
    10921 tcagagacag ctctattaaa tatattgccg aagcagaaga attatacaaa gatagcacaa
    10981 aagcaggtaa agaaaaattt tcttgggttg tcgatgcctt atatgactta atacctgctc
    11041 ctttaaaaat gtttataaca aaaaaatgta ttgaaaaaat cgttcaatcc tcctttgatg
    11101 caatcgagga ttatgcaaaa atgcaatttg aaatagcaat tgaaaaatac cttaaacatc
    11161 ttagcgatgg aaaatcaggg gacttcattg attttataaa gagtgattgt gaatgattcg
    11221 attaaaacac ctgcccttag acgatatttc aataagcagc ccctacggag ccagaagtat
    11281 aactgttaac ggcagatact attggtggca caacggattg gatttaaaag cacagcttaa
    11341 tactcccgta tatgcagtag cagatggaga ggtgatggct gcaagatacg ataacagcta
    11401 cggctactat atagccatag accacggcaa atttggcacc ttgtatgctc atctttcaca
    11461 ttacaaagtt actgaaggaa gtattgtaaa atcaggtgaa ataatcggcc ataccggcaa
    11521 caccggcgat actacaggtc cccacctgca ttttgaaata agacttggaa gttacgcaaa
    11581 cttctgggag agagcacact gtgacagaag tgtttttatg aacactatag accccatgct
    11641 ttttatagaa gactttttta ataaaaaaga taatttaagc gtagaagaag ctgcaaagat
    11701 tgtccagtcc gctgccggat taga
//
LOCUS       DAOSHP010000057        11631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4655_length_11631_cov_7.118521, whole genome shotgun sequence.
ACCESSION   DAOSHP010000057 DAOSHP010000000
VERSION     DAOSHP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_11631_cov_7.118521"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..796)
                     /locus_tag="PLB33_06500"
     CDS             complement(245..796)
                     /locus_tag="PLB33_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63210.1"
                     /translation="MKKKTDFILGMLFAAATIIFIVIFLTNNIFFNWAFERHHNILSW
                     YIRPLFIIPMVIFAFKKSLTGIFASIFALFTSMFWFPVPAEKSPQVLEFLAFEMDYLK
                     GAWTAPKIIMSLAVPLFFFMLLTAAWKRNLKWLLGVVIGAALLKIIWSIAFSGEAGMS
                     ILKPALIGLIVCIVGLYYYFKKH"
     gene            1023..1601
                     /locus_tag="PLB33_06505"
     CDS             1023..1601
                     /locus_tag="PLB33_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOG63211.1"
                     /translation="MKNINTANKKELTIEQCEEIIKILKDRFEKNMNRHKDLVWDKIQ
                     VKLKANPGKLWSLNEMEKTGGEPDVIAYDENTDEYIFFDCSPESPIGRRNFCYDNEAL
                     ESRKKNKPENSAINTAHEMGIEILTEEQYRELQKLGSFDLKTSSWVKTPDKIRKLGGA
                     LFCDRRYDTVFVYHNGAESYYGARGFRGSLRV"
     gene            complement(1615..2388)
                     /locus_tag="PLB33_06510"
     CDS             complement(1615..2388)
                     /locus_tag="PLB33_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63212.1"
                     /translation="MIKLNNVTSGYMGVNIIKNISLSFEKGKIISIIGKNGCGKTTLL
                     KTASKIIEPYSGDILLNGKNLFDMTSKESAKIISYLPQNRDIPNIRVYDLVMHGRFPY
                     LGFPRIPAYEDKIKVQEALHVLEIEKYKNKNIRELSGGQRQKVYIAMVLAQDTDIVFL
                     DEPTTYLDINHQLEILEIVKDLKKMGKTVVMVIHDLNSALTYSDMVCLMDKGEIKIYD
                     KPQVVYESGKIEEIFNVKCHEVGAYDKYEKQYIFSYMSA"
     gene            complement(2381..3433)
                     /locus_tag="PLB33_06515"
     CDS             complement(2381..3433)
                     /locus_tag="PLB33_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG63213.1"
                     /translation="MKAIDTLQKLSIPKSLINYKKAYNFFVISFLLILLIVLIFTSIS
                     VGSSSIEISDIFNAISGNGQLTSEYRIINYVRIPRTLSAIIAGSGLAISGLILQSVLN
                     NALASPTIIGVNSGAGLFTALATAFFPANLSIMPLAAFLGAFLATIAVYYVARKTGAS
                     RMTIILAGVAVSSLVGAMTDTVLILFPDTVISRTSFIIGGLSGVTMNRLLMPGIIIFF
                     SLITTFIFSYDLNVLSLGDQTSKSLGLNVDKTRFLFIILSSLLSGSVISFAGLLGFIG
                     LIVPHISRLIVGYDNRILIPTSVLIGSSFALFCDILARTIFAPYEVPVGIIMSFLGSP
                     FFIYLLLNKKRRLMYD"
     gene            complement(3381..4361)
                     /locus_tag="PLB33_06520"
     CDS             complement(3381..4361)
                     /locus_tag="PLB33_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63214.1"
                     /translation="MISKKKNLFLILTLILMLITAACSKETNNVEAINIDNQDAEENY
                     YIFSDALGTEVVLERKPERVVSLLGSYSETWALAGGSLAGVTDDVQKEGRMEITNDIQ
                     IVGTVKEPNLEGILSLNPDFVILTPDVDNQVKIAETLKQLNINHAFFKVEEFEDYLNM
                     LNICTDITGRKDLYEENGLKIKKDIDDILYKVEGAKKEELKVLFVRAFSSGAKAKKDD
                     NMTCTMLDNLGTVNIAAQHPSLLEDISIEEIIEEDPDFIFVTTMGDEQKAIDALKTGI
                     GTNPAWNSLSAVENDRYIILPKELFHYKPNAKWGESYRYLAEIIYPEIFN"
     gene            complement(4501..5421)
                     /locus_tag="PLB33_06525"
     CDS             complement(4501..5421)
                     /locus_tag="PLB33_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG63215.1"
                     /translation="MTIRHLKIFIEVAQCGNMTAAASKLFISQPTASQAIRELEEHYG
                     VLLFERLSKRLYITEAGKKLLFYAKEVVKEFDSLESKMNNLNKKDKIRVGATISVGDC
                     ILSDLIHRCIEENPNVEIYSFVGNTETIEEKLLSNELDIAIVEGKVKSQDLIVKPELE
                     DYLVLICSTKHPFAQKKIIKTDELRDENFAMREKGSGTRELFEEYMLNNGMPIKVVFE
                     GNTPQSIKQEVIHNNCLSVISICLVENEIKDSLIHVIENEENTWNRHFSLVYHKDKLL
                     TDAIKSLINIVKDYRYIPSKAIVNPGILRK"
     gene            5555..7177
                     /locus_tag="PLB33_06530"
     CDS             5555..7177
                     /locus_tag="PLB33_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG63216.1"
                     /translation="MNIIIIGGVAAGTKVAAKLKRDNYNHNVKILTKGKDISYAGCGL
                     PYYVGNVIEKQEDLIQNTPKQFSELTGVEVLTEVEVTKVNPEEKTVEALNLITNETAT
                     YSYDILVIATGAEPIKPPIEGIDLDGVYFMRTPEDAIKLREAVDEGKIKRAVVVGGGF
                     IGLEVAENLTAQGVNTSVIDMAGQILPGFEPEIANYVDNYLADQRIITFTETGLEAIL
                     GDGKVEKIKTNNRTMKADAVVLSIGIRPNTAFLNGSGIELAPNRAIKVNEKLETNIKD
                     IYAVGDCAFVTNIITGKGAYAPMGSTANIEGRILAQNINGNNLNYKGAIGTAVVKLPG
                     INAGKTGLTEDAAKSEGYNVETVTTVMEDKAKYYPGASVFIIKMIADKVSKKLLGVQV
                     VGEGNIDKIVDMAVTAIGLDAKLEDIQNLDYAYAPPFSTAIHPFAATVNVLLNKINGN
                     LVSVTPREYKEGKADGYKIIDSSIVPKITGAPYVDISKAHEELPEFDKDEKLLLVCDK
                     GKRSYMLQQRLKDFGYTNTLVLEAGTMFNELK"
     gene            7210..8160
                     /locus_tag="PLB33_06535"
     CDS             7210..8160
                     /locus_tag="PLB33_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOG63217.1"
                     /translation="MANLKVTPAEEKTVKALGFLRNKGTDNFSARIITVNGKITAAQQ
                     KIIGEAAEKFGNGDVTFTSRLTIEVPGIPYEKITEFQDYIGKEGLVTGGTGSKVRPVV
                     ACKGTVCHHGLIDTFDVSEDIHNIFFEGYAPVKLPHKFKIGVGGCPNNCMKPSLNDLG
                     VIGQMVPNYDEELCMGCKKCSVEEACPIGAAKVVDGTLVIGDDCNNCGKCIGTCNFDA
                     IPDGDKGYKVFIGGMWGKRVNPGVPINKIFKTKEEVFEIIEKMILLYREQGQTGERVA
                     QTVNRIGFENVEAQLLSDEIFERKQEILEAELHMTGGATC"
     gene            8311..9351
                     /locus_tag="PLB33_06540"
     CDS             8311..9351
                     /locus_tag="PLB33_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOG63218.1"
                     /translation="MNKKKLFAIIVCIIIALVATYIGGAQKIIGAPMLGLLIGMAVVN
                     MIPSVDKDFKSGTSFAGKKFLNLGIILTGATLNFNQILGYGARAMPLLLFNICVAFLV
                     SFTIGKKLRVSANTSTLVGSGSCICGGTAIATMSSVIGAKETEIAYAMTAIFLFDILS
                     ALLYPYLATALNLTQNQFGFLAGTSINDTSSVVAAESTYNVLNNIDSSLAVTIKLART
                     TLLILLVVVFTVLKAKEQSVTNVAGESSKNVSIGRTVIKTFPKFILVFLLMAVLNTIG
                     VFDGISGAAVFFKKYSKFFITTALAGVGFKIQFKDLFTEGLKPIVLGGITWLSICISS
                     MLFITIFASYVG"
     gene            9585..10412
                     /locus_tag="PLB33_06545"
     CDS             9585..10412
                     /locus_tag="PLB33_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOG63219.1"
                     /translation="MKFISWNIDSLNAALTGDSPRAKLSQNVLNTIKEYNPEVIALQE
                     TKLSSNGPAKKHLQVLDEMFPDYEIVWNTSVEPARKGYAGTMFLYKKDLKPTVSFPKI
                     GAPGTMDAEGRIITLEFDNFYLTQVYTPNAGDNLNRLEDRQVWDIKFADYLESLDKEK
                     YVIATGDFNVAHKEIDLAHPDNNRRSAGFTDEEREGFTNLLNRGFTDTFRYIHGDVPG
                     VYSWWAQRAVTSKINNSGWRIDYWLVSNRIADKVIKSEMIDSGPRQDHTPILLEICL"
     gene            10485..11513
                     /locus_tag="PLB33_06550"
     CDS             10485..11513
                     /locus_tag="PLB33_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOG63220.1"
                     /translation="MSKVYFIRNTESDYSKLGKDAAELLKRIVSETGHKFEKEVPIKV
                     HFGEKGNKTFIPAVCYDDTIKYLKENDVSPFYIESNVLYRGSRTTTQKHIETAKSHGF
                     TQIPIIIADGDIGTEYNEIEINKEYIKTCKIGKAYGDYKQFIVMSHFKGHVEAGFGGA
                     IKQLGMGFASRGGKLEQHSGISPVVNEKKCISCGICIDKCNYSAITKTNVAVIDSSKC
                     IGCAGCIAVCPQGAIRNTWGGSNFFEKLAEYAYGAAKDKDIIFIAFVHNITKDCDCNG
                     THMKLIADNVGILAGKDPVSLDTACLDLVQKTEGIKLFDKGRNTLKHAEKIGLGEMNY
                     ELIKIKIP"
     gene            11510..11631
                     /locus_tag="PLB33_06555"
                     /pseudo
     CDS             11510..11631
                     /locus_tag="PLB33_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
BASE COUNT         3921 a         1894 c         2120 g         3696 t
ORIGIN      
        1 taggggaaca aaaccgtacg atttctttga ttatgttaga tattgatgac tttaatgcga
       61 tgtcaaccga aaaaacaggc aacataatga acttgttcaa agaggcagat gcgaatatgt
      121 atcaggataa agccctgtat catcaagggg cagaaactta aaaatatttt tctcctttag
      181 atattggagg tattaaacat gaacattaca tttccaataa agagcactat tgattctctg
      241 ttcgctaatg ttttttaaaa taataatata atccaacgat gcagactatt aaaccgatta
      301 atgcgggttt taaaatagac attcctgctt cgccgctgaa tgcaatactc caaataattt
      361 tcaaaagagc tgcacctatg acaacaccta aaagccattt taggtttctt ttccatgctg
      421 ctgtaagaag cataaaaaag aatagaggta cagctaaact cattattatt tttggtgctg
      481 tccatgcacc ctttaagtag tccatttcaa aagccaaaaa ttctaaaacc tgaggacttt
      541 tttctgccgg aacaggaaac cagaacatac ttgtaaatag agcaaatata gaagcgaata
      601 taccggtcaa acttttctta aaagcaaata taaccattgg tattataaat aagggtctga
      661 tataccagct taatatatta tgatgtcttt caaaagccca attgaaaaat atattattgg
      721 taagaaatat tactataaaa attatggtag cagctgcaaa tagcattcct aatataaaat
      781 ctgttttttt cttcatgcta agcatcctta aataattaat tttgttatga attccattat
      841 aatatattta taattgaata aacaatatta ttattaagtt tttaatttat aactaacttt
      901 atctttgaat acagggtttt gttttttttt gctttttaat aatggcaggt tttaaatttg
      961 tgtgtttatg ggtatataaa taaaaaacca aattaaacta atgttatcag gaaggaatcc
     1021 atatgaaaaa tataaatact gcaaataaaa aagagttaac catagaacaa tgtgaggaga
     1081 taattaaaat attgaaagac cgctttgaga aaaacatgaa tcgtcacaaa gaccttgtat
     1141 gggataaaat acaagtaaag ctgaaagcta atcccggaaa gctttggtca ctaaacgaaa
     1201 tggaaaaaac cggcggagag ccggatgtta ttgcttatga tgaaaatacg gatgaataca
     1261 ttttttttga ctgttctccg gaaagcccca ttggcagaag aaatttttgc tatgacaatg
     1321 aagcattgga gtcaagaaag aaaaacaaac ctgaaaacag cgcaattaat acagcccatg
     1381 aaatggggat tgagatttta accgaagaac agtaccgaga attacagaag cttggaagtt
     1441 ttgacctgaa aacatcaagc tgggtcaaaa cacccgataa aatcagaaaa ctcggaggcg
     1501 ccttattttg tgatcgccgc tatgatactg tgtttgtata ccacaatgga gcagaatcct
     1561 attatggtgc caggggattt cgaggctcgc taagagtata gcactcgagt gcgattaggc
     1621 tgacatatat gaaaatatat attgtttctc atatttatca taggcaccta cctcatgaca
     1681 ttttacgtta aatatttctt ctatttttcc gctctcatat accacttgag gtttatcgta
     1741 aatttttatt tctcccttat ccattaaaca aaccatatcg gaataagtga gggcgctgtt
     1801 taagtcatgt ataaccataa caacagtctt gcccattttt tttaaatctt ttacaatttc
     1861 caaaatttcc aactgatgat ttatatctaa gtatgtggtc ggctcgtcca agaaaactat
     1921 atctgtatct tgtgctaata ccattgctat atatactttc tgcctttgtc cgcctgaaag
     1981 ttctcttatg tttttgtttt tatatttttc aatttccaat acatgaaggg cttcctgtac
     2041 tttaatttta tcctcatatg caggtattct cgggaatcct aaataaggaa atctgccgtg
     2101 cataactaaa tcataaactc ttatattggg tatatctctg ttttgaggca ggtatgaaat
     2161 aattttggcg gattctttgc tcgtcatatc aaatagattt tttccgttta ataaaatatc
     2221 tccggaatag ggctcaataa ttttcgaagc tgtttttagg agggtggttt tcccgcatcc
     2281 gtttttaccg ataatgctta taattttgcc cttttcaaag gacaggctta tatttttaat
     2341 tatattgaca cccatatacc ctgatgttac attattcaat ttaatcatac ataagcctcc
     2401 ttttcttgtt aagcaataaa tatataaaaa acggacttcc caaaaatgac ataataattc
     2461 caacaggtac ctcataaggt gcaaatatag tacgagccaa tatatcgcaa aataatgcaa
     2521 aagagcttcc tattaaaaca cttgtcggaa ttaatattct gttgtcatag ccgactataa
     2581 gccttgatat atgtggaact ataagaccta tgaagcctaa gagccctgca aaacttatga
     2641 cactgcccga taacagtgaa gacagtatta taaataagaa acgggtttta tctacattta
     2701 gtccaagaga cttgctagtt tggtctccta aggataaaac atttaaatcg taactgaata
     2761 taaatgttgt tataagcgaa aaaaatatta tgattccagg cattaagagc ctgttcattg
     2821 taactcccga tagtccgccg attataaatg aagtcctgct tattacagta tccggaaaaa
     2881 gaattaaaac cgtatcggtc attgcaccta caaggctgga taccgcaaca cctgccaata
     2941 taattgtcat tcgtgatgcc cctgtttttc ttgccacgta atatactgct atggtggcca
     3001 aaaatgcacc taaaaatgca gctaggggca ttatgctaag gttagcagga aaaaaggctg
     3061 tagccagtgc tgtaaacaga cctgctcctg aatttacccc aattatagtg ggacttgcca
     3121 aggcattgtt taacacagac tgtaaaatca gtccggatat agctaatccg cttcctgcta
     3181 ttattgctga cagggtcctt ggtatccgta catagttaat aattctatat tcagatgtta
     3241 actgaccatt accggatatg gcattaaata tatcggatat ttcgatactt gaagaaccga
     3301 cggatatgct agtaaaaata agtactatta ataggattaa cagaaagctt ataacaaaaa
     3361 agttataagc ttttttgtaa ttaattaaag atttcgggat agataatttc tgcaaggtat
     3421 ctatagcttt caccccattt tgcatttggt ttatagtgga acaattcttt tggcagtatg
     3481 atatacctgt cattttctac agcagataag ctgttccagg ccggatttgt tcctatgccg
     3541 gtttttagtg catctattgc tttttgttca tctcccatag ttgttacaaa tatgaaatca
     3601 ggatcttctt caataatttc ttcaatgctt atatcctcca ataatgacgg gtgctgagct
     3661 gcaatgttga cggttcctaa attatccagc attgtacaag tcatattgtc atctttctta
     3721 gcttttgctc cgcttgaaaa ggcacgaaca aataaaactt taagttcttc tttttttgca
     3781 ccttctacct tatacaatat atcatcaata tcttttttga ttttaagtcc gttttcttca
     3841 tataagtctt ttctgcctgt tatatcagta catatattta acatgtttaa ataatcttca
     3901 aactcttcca ctttaaaaaa agcgtgatta atatttaatt gttttaaggt ttctgcaatt
     3961 ttcacctggt tgtcaacatc gggagtcagt attacaaaat cagggttcag tgataatatt
     4021 ccttctaaat ttggttcctt tacggtacct acaatttgaa tgtcatttgt tatttccatg
     4081 cgtccttcct tctgaacatc atcggtaaca cctgctaagc ttccacctgc caaagcccag
     4141 gtttccgaat aggaccccaa taaagatact actctttcag gttttctttc taaaacaacc
     4201 tctgtaccca atgcatctga aaatatgtaa taattttctt cagcatcttg attatctata
     4261 tttattgctt ccacattgtt tgtttctttg ctgcaagctg ctgtaatcag catcaatatc
     4321 agcgttaata taagaaacag atttttcttt ttgcttatca tactaccacc atctttccta
     4381 taaaaaaata tattatcatt gttaattaat acacatactt tatgtttata ataacatcta
     4441 agcagtttaa ttaaaaataa taatatatta tcactgttat aggtaaaacc tattgattaa
     4501 ttactttctt aaaatacccg gattaactat tgctttagag ggtatatacc tataatcctt
     4561 tacaatattt attagagact ttattgcatc agtcaataat ttatctttat ggtatactag
     4621 gctgaagtgc ctgttccaag tgttttcttc attttcaata acatgaataa gagaatcctt
     4681 tatttcattt tctaccaaac aaatagatat aactgacagg caattgttat gaataacttc
     4741 ctgcttgatg gattgcgggg tatttccttc aaaaacaacc tttatgggca ttccgttatt
     4801 aagcatgtat tcttcaaata gctccctggt tccgctgcct ttctctctca ttgcaaaatt
     4861 ttcatcacga agctcgtctg ttttaattat ctttttctga gcaaaggggt gtttagtact
     4921 gcatattaag actaaataat cttctaattc aggcttaaca attaagtctt gacttttaac
     4981 tttaccttct actattgcaa tatccaattc attgcttaat aatttttcct ctatcgtttc
     5041 agtgtttcct acaaaagaat atatttctac atttggattt tcttctatgc atctgtgtat
     5101 taaatcgctt aaaatacaat ctccgacaga tattgtagcc ccaactctta ttttatcttt
     5161 cttgttcaaa ttattcatct tactctctaa gctgtcgaat tcttttacca cttcttttgc
     5221 ataaaacaat agttttttac ctgcttcagt aatatacagc cttttgctga gccgttcaaa
     5281 taacaatacc ccgtaatgct cttctaattc tcttatagct tgacttgccg taggttgtga
     5341 aataaaaagc tttgaagctg ctgcagtcat attgccgcat tgtgccactt caataaatat
     5401 ttttaaatgc ctgatagtca tgagaactct ccttttcaac atcataggtt ttgcctatga
     5461 ttatgataag attttatcat tttactaatt ataaagcaag tgttatatta tacataaatg
     5521 tcttgttaaa taatacacaa aggaaggtgc gtttatgaat attattataa ttggaggcgt
     5581 agctgcaggt actaaggtag cggcaaagct aaagcgtgat aattataacc acaatgtaaa
     5641 gatacttact aagggtaaag atatatctta tgccgggtgc gggctgccct attatgtagg
     5701 aaatgtaatt gaaaaacaag aagacttaat ccaaaataca ccaaagcaat tttctgaatt
     5761 gacaggtgta gaagtattga ctgaagtaga agtaactaag gttaatcctg aagaaaaaac
     5821 cgtagaagca ttaaacttaa ttacaaatga aactgctaca tattcatatg acatattagt
     5881 aattgcaacc ggtgctgaac cgattaaacc tccgatagag ggaattgacc ttgatggagt
     5941 atactttatg agaactcccg aagatgccat aaaattacgt gaagcagttg atgaaggcaa
     6001 aataaagaga gcagtagttg tcggtggcgg atttatagga ttagaggttg ccgaaaactt
     6061 aacggctcaa ggcgtaaata cttcggtaat tgatatggcc ggacagattc tccccggatt
     6121 tgaaccggaa attgcaaatt atgttgataa ctatttggct gaccaacgaa ttataacatt
     6181 taccgaaacc ggtttagaag ctatattggg cgatggaaaa gttgaaaaaa tcaaaacaaa
     6241 caatcgtacc atgaaagcag atgcagtagt tttatcaata ggtataaggc ctaatacagc
     6301 ttttttaaac ggtagcggta ttgaattagc accaaataga gctatcaagg ttaatgaaaa
     6361 acttgaaacc aatatcaaag atatttatgc agtaggtgat tgtgcttttg ttacaaacat
     6421 tattaccggt aaaggtgctt atgctcccat gggctctact gccaatattg aaggaagaat
     6481 attagcacaa aacataaatg gaaacaattt aaattacaaa ggtgctattg gaacagcagt
     6541 tgttaaactt ccgggaatta atgccggaaa aaccggtttg acggaagacg ctgctaagtc
     6601 agagggttat aatgtagaaa ctgttactac cgtaatggaa gacaaagcaa aatattatcc
     6661 cggtgcttca gtttttatca taaagatgat tgcagataaa gtttcaaaga aactgttggg
     6721 tgtacaagtt gtcggtgaag gcaatattga caagatagta gatatggctg ttacggcaat
     6781 cggattagat gcaaagttag aagatattca aaatctcgac tatgcttatg ctcccccatt
     6841 ttcaacagca atacatcctt ttgcggctac cgtaaatgtt ttattaaata aaataaacgg
     6901 taatttggtt tctgttacac caagagaata taaagagggc aaggctgacg gatataagat
     6961 tatagattca tcaatagttc ctaaaattac aggtgctcct tatgtagata tttcaaaggc
     7021 acatgaagaa ttgcctgaat ttgataaaga tgaaaaacta ttgttagtat gtgataaagg
     7081 caagagatcc tatatgctgc aacagagatt aaaagatttc ggatatacaa ataccttagt
     7141 acttgaagcc ggtactatgt ttaatgaatt aaaataaata atatagattt agaagaggag
     7201 aataaaatta tggcaaattt aaaggttaca cctgcagaag aaaagactgt taaagctctt
     7261 ggttttttga gaaataaggg tacagacaat ttttctgctc gaattattac tgttaacgga
     7321 aagattacag cagctcaaca aaaaataata ggagaagctg cagagaaatt tggaaacgga
     7381 gatgtaacat ttaccagcag gttgacaatt gaagtaccag gcatacctta tgagaaaatc
     7441 actgaattcc aagattatat cggtaaagaa ggattagtta caggaggtac aggctctaag
     7501 gttcgtccgg tagtagcttg taagggtaca gtttgtcatc atggattaat tgatactttt
     7561 gatgtatcag aagatataca caatatattt tttgaaggtt atgcaccggt aaaattaccg
     7621 cataaattca aaataggtgt aggcggatgt ccaaataact gtatgaagcc ttcattaaat
     7681 gacctcggcg taatcgggca gatggtgcct aactatgatg aagaactttg catgggttgc
     7741 aagaaatgct ctgtagaaga agcatgtcca attggtgctg caaaagttgt tgatggtaca
     7801 ttagtaatcg gtgacgattg caacaactgc ggaaaatgta taggaacttg taatttcgat
     7861 gccattccgg atggtgataa aggatataaa gtatttatcg gcggaatgtg gggtaaaaga
     7921 gtaaatcccg gagttcccat aaacaaaata tttaaaacaa aggaagaagt attcgaaatt
     7981 attgaaaaaa tgatactcct ttacagggag caaggacaaa caggtgaaag agttgctcaa
     8041 acagtaaaca gaatcggatt tgaaaatgtt gaagctcaat tgttatctga tgaaatattt
     8101 gaaagaaaac aagaaatatt agaagctgaa cttcatatga caggcggagc tacctgctaa
     8161 agaaaactat atccggaaga atatttcttc cggagataat tttctcatac ttcgttaaaa
     8221 aaataattaa caaaccggag aaagattaaa ttttaagtat ggtgtttttg aacgcttggt
     8281 attattatgt aattagaagg agaaaatgtc atgaataaga agaaactttt tgcaataatt
     8341 gtctgcatta ttatagctct tgttgcaaca tatataggtg gagctcaaaa aataatcggt
     8401 gctccaatgt taggactatt gattggtatg gctgtagtaa acatgattcc gtctgtagac
     8461 aaagatttta aatcaggtac aagctttgca ggaaagaaat ttttgaattt aggtattata
     8521 ttaaccggtg caacacttaa tttcaatcaa atattaggct acggggcaag agctatgcct
     8581 ttattattat ttaacatatg tgtagctttt ttagtttcat tcactatagg taaaaaactt
     8641 agagtatccg ccaatacatc aaccctggta ggcagtggtt cttgtatttg cggcggtacc
     8701 gcaattgcaa caatgtcatc tgttattgga gcgaaagaaa ccgaaatagc ttatgcaatg
     8761 acagctatat ttctgtttga tatactttct gcgttattat atccatactt ggcaactgct
     8821 ttaaatttaa ctcaaaatca attcgggttt ttagccggta caagcattaa tgatacttca
     8881 agtgttgtag ctgctgaatc tacatataat gttttaaata atattgattc aagcttagcc
     8941 gttacgataa aacttgcaag aacaacatta ttaattcttc ttgtggttgt atttaccgtc
     9001 ttaaaagcaa aagagcaatc tgtaacaaat gttgccggtg aatcaagcaa aaatgtgtca
     9061 ataggaagaa ctgttattaa aacattccct aagtttattc ttgtattttt attgatggct
     9121 gttcttaata caatcggtgt atttgacgga atcagcggag cagcagtatt ctttaaaaaa
     9181 tactcaaaat tctttattac cactgcttta gccggggttg gatttaagat tcagtttaaa
     9241 gacctattta ctgaaggcct taagcccata gtattaggtg gaattacatg gttatctata
     9301 tgtatatcaa gtatgttatt tattacgata tttgcaagct atgttgggta gattaattta
     9361 aaacttgagc tttattagtc aaattcccta atgtgttgat agtatagtcc cgaccataat
     9421 attttattat ggcgggactc tttcttaaac gaactttgtt gaacgaatta tgcaagcgga
     9481 atggttacgg taaaagagat aaagattgca gattcttatt aaatagttta aaataaaaga
     9541 aacataataa ctatataatt acaaaaaata agggggataa ggcattgaag tttatttcat
     9601 ggaatattga ttcattaaat gcagcattaa caggtgattc tccacgtgct aaattatctc
     9661 aaaatgtatt aaatacaatt aaagaatata atccggaggt tattgccctt caggaaacaa
     9721 agctatcaag caatggtcct gctaaaaaac atttgcaagt cctggatgaa atgtttcccg
     9781 actatgaaat agtttggaat acttcggtag aaccggcccg caagggttat gcgggcacca
     9841 tgtttctata taaaaaggat ttaaaaccaa ctgtttcctt tccgaaaatc ggggcaccgg
     9901 gtactatgga cgctgaaggc agaatcataa ctttagaatt tgacaatttt tatttaaccc
     9961 aggtttatac tccaaacgcg ggggataatt taaatcgttt ggaagaccgc caggtttggg
    10021 atataaaatt tgcagactat ttggaaagtt tggataaaga aaagtatgtt attgcaacag
    10081 gtgattttaa tgtggcacac aaggaaatag acttggctca tcctgacaat aaccgtcgtt
    10141 cagccggttt taccgatgag gagcgtgaag gctttacaaa cctgttgaac agaggtttta
    10201 cggacacttt ccgttatatt cacggtgatg tgccgggtgt ttattcttgg tgggctcagc
    10261 gtgccgtaac cagtaaaatt aataattccg gttggagaat tgattattgg ctggtaagca
    10321 atcggattgc agataaggtt atcaagtctg aaatgattga ttcaggccca agacaagacc
    10381 atacacctat tttgcttgaa atatgcttat agcaattcta actaattccc ttggaccaaa
    10441 gtttgaggga atgataaaaa taatatgaaa gagaaggtgc acaggtgagt aaagtatact
    10501 ttattagaaa taccgaatct gattatagta aacttgggaa agatgcagca gaactattaa
    10561 aaagaatagt ttcagaaaca gggcataagt ttgaaaagga agtacctatt aaggttcatt
    10621 ttggggaaaa gggaaacaaa acattcattc ctgccgtatg ctatgatgat actataaaat
    10681 acttgaaaga aaatgacgtc tcaccctttt atattgaatc taacgtatta tacagaggtt
    10741 caagaacaac tacccagaaa catatagaga cagcgaaatc acacggtttt acgcaaattc
    10801 cgattataat agcagatgga gatataggaa ctgagtataa tgaaatagaa ataaacaagg
    10861 aatatataaa aacatgtaag attggcaagg cttatggaga ttataagcag tttatagtta
    10921 tgagtcattt taagggtcat gtagaagcag gctttggagg tgcaataaag caacttggta
    10981 tgggttttgc ctcaagggga ggaaaacttg agcagcattc aggcatatcc cccgtcgtaa
    11041 atgaaaagaa atgtatatcc tgcggcatat gtattgataa atgcaactac agtgcaataa
    11101 caaaaacgaa tgttgctgtt attgacagca gcaaatgtat cggttgtgcc ggttgtattg
    11161 cggtttgccc acaaggtgcc attaggaata catggggcgg ttcaaacttc tttgagaaac
    11221 ttgcagagta tgcctatgga gcagcaaagg acaaagatat tatatttatt gcatttgttc
    11281 ataatatcac taaggattgt gactgcaacg gaacacatat gaagctgatt gcagataatg
    11341 tcggtattct tgcaggtaaa gatcccgtat ccttagatac cgcatgttta gatttagtac
    11401 agaaaacaga gggcattaag ctttttgata aaggcaggaa tactttaaag catgcggaaa
    11461 aaatcggcct gggtgaaatg aattatgagc ttattaaaat aaagatacct taatagatgt
    11521 gcatattgtg gttgagcgcc ttgttcccaa gctggccggt ttaactttct gtaaaaaggg
    11581 acaaacaagt gaattggcaa caaaacgcta tcatgaaatc cttagaaatc t
//
LOCUS       DAOSHP010000058        11585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4677_length_11585_cov_9.762966, whole genome shotgun sequence.
ACCESSION   DAOSHP010000058 DAOSHP010000000
VERSION     DAOSHP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11585
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4677_length_11585_cov_9.762966"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLB33_06560"
     CDS             complement(<1..607)
                     /locus_tag="PLB33_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HOG63221.1"
                     /translation="MKKNDIIEVAINDLEFPNIGTTNFENKKIRIKNTLPGQKVRAKV
                     VRSRKESADARLIEVLENAYYEKEPLCEHFNLCGGCTYQTVPYKNQLEIKEKQVKNIL
                     DEILTEKYEFLPIVPSPKETEYRNKMEFSFGDEIKDGPLVLGMHKRNSFHGIVNTHGC
                     QIVDEDFRRILCHILEHFLGNSQTYYHKTTNIGFLRYLLVRK"
     gene            complement(740..1321)
                     /locus_tag="PLB33_06565"
     CDS             complement(740..1321)
                     /locus_tag="PLB33_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOG63222.1"
                     /translation="MSKIVSIVFHSICGNNFLISKEFYKEFKAHNADVQLLRVKDINF
                     DELAKQFTIGTQYKKEILNIEEAKVEKLLDSDIIILGSPTYFGNVSGAMKAFMDEFYP
                     YWQSEKFYGKKMLAFTTCANGEGGGDLCLNAMTVFAQNMGMITVPVPKNLVSGQNFPA
                     NGLIHYVGDYSNLRPSGKIIPAIKEMTQKLMEM"
     gene            complement(1318..3072)
                     /gene="ptsP"
                     /locus_tag="PLB33_06570"
     CDS             complement(1318..3072)
                     /gene="ptsP"
                     /locus_tag="PLB33_06570"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOG63223.1"
                     /translation="MKGLGVSPGIGIGKAVVIDKASINITKNYIKDAGNELDRLMNAI
                     EAAKEQLNELYVSTIDELGEKEAQIFKSHEMMLEDDTFISDVENRIKQEGVNAEYALN
                     ETSNVYIKMFQSIEDEYLRERAEDIKDVMNRVIRILMGISSVDFSNLKENSIIVAKDL
                     TPSDTAQIDRTKVAAMITEIGGKTSHAAIIARIMGIPTVVGLNNITDKIKNGDTVICD
                     GKTGKVLVNPNAKQLFYFTKKKNIEDEIRNELKNQIGLPSVTKDGFKISLTANIGVPN
                     DVDMALENDAEGIGLFRSEFIFMNREHQPTEDEQFEQYKEVLVKMGDKPVIIRTLDIG
                     GDKNVPYIDIPKEMNPFLGYRAIRLCLGNVEVFRTQLRAILRASVYGNVKIMIPMIST
                     MKELKDSKKILQQAKDELTKEGIKFKKDIEIGIMIEIPSAAIISDLLAREVDFFSIGT
                     NDLIQYTMAVDRMNSKLSYLYSQYHPALLRLIKGIIDNAHEAGIWVGMCGEAAGDPKL
                     IPVFVGMGLDEFSMNSPSILRARYIVRNLNKADMELIAKSTLNMENALEVEEYLSCLF
                     ADENESCLRTCKNRRRQE"
     gene            3401..4144
                     /gene="aroD"
                     /locus_tag="PLB33_06575"
     CDS             3401..4144
                     /gene="aroD"
                     /locus_tag="PLB33_06575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOG63224.1"
                     /translation="MENKRIKNINLNGIPKICVPVTGVTENEIIDEFSKLKNAPIDLV
                     ELRADYFEFVQDQTKVTDILQQIRKLYEKPLLFTLRTKNEGGVSYIDADYYFKLNNSV
                     IESKLVELIDIEFFSQAEKVDKTVKLARKNNVMTILSNHDFLKTPPEEEIISRVNKMM
                     DCGDIAKIAVMPNSEEDVLTLLSAALKLKKEKKGHFIAISMGPLGVISRISCELIGSC
                     ITYASYGDKSAPGQLDVRLVKELLEIMHV"
     gene            complement(4141..5445)
                     /locus_tag="PLB33_06580"
     CDS             complement(4141..5445)
                     /locus_tag="PLB33_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG63225.1"
                     /translation="MKNKIIIIGVVLIFLIAASYLIPQEVPMINIDDSIKMYIVADPH
                     YMSEKLTEDSEVFNNYLNTLDRMMKYTSVFLDIIEDDVKKNQPDIVVFPGDLTNNGSK
                     VNHLEFEERLKSIKSTGAKVYVVPGNHDINNEKALYFKDNEIRITESINKEEFVEIYK
                     SYGYNEAISRDENTLSYLVKPYKNLWLLMLDTTKDYPEPGGYINRDTLKWIASCSDMA
                     KEENAEIIVVMHHNLLDHSDIIWEDYTVDNNTSVINTFHECDIQLVLSGHIHIQDVKT
                     HTSVETGNTIYDIATSSLPVFTHQYGQLTYSPSRGYDYETIKLNVEEYALKNNIEDEN
                     LLSFNEYAEKFFVENLCRTHKDSIGLLDIPDEHKEEVFKTVTEMNKKYFSGYRNEALK
                     QLIETEGFKIVEELDECFIQEYIMGMIKDQNADNNKLHIPIN"
     gene            complement(5623..8748)
                     /gene="ileS"
                     /locus_tag="PLB33_06585"
     CDS             complement(5623..8748)
                     /gene="ileS"
                     /locus_tag="PLB33_06585"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOG63226.1"
                     /translation="MKLFKDISKADYKNNEHEVSKYWEEINLLEQSIETRKNKPSFVF
                     YEGPPTANGRPGIHHVISRTLKDSVCRYKTMQGYQVKRKAGWDTHGLPVEIEVEKQLN
                     LSTKQEIEEYGIDEFNKKCRESVFTYEALWREMTERMAYLIDLDNPYITLENDYIESE
                     WWILDKVFKEGLIYEGHKILPYCPRCGTGLASHEVAQGYEEIKTITAYAKFKKKDEDA
                     YFLAWTTTPWTLLSNVTLTVHPDVDYVKIRTKDKEDVKGETYILAKTLVDKVINEEYD
                     VLDEKKGSELEFVEYEQLLPYVEVPAGKKAFFVTLADYVTVEDGTGIVHTAPAFGEDD
                     YNTGLKYNLPVLNPVDDTGRFRGTPWSEMFVMDADQPILRWLKENGKLYKKEPVLHNY
                     PHCWRCHTPLLYYARPSWYIQMTKLKDLLVANNNTVNWYPDYVGEKRFGNWLENVNDW
                     AISRSRYWGTPLPIWRCECGHIESIGSRKELVEKAVEDIDESIELHRPYVDEVHIVCP
                     ECGKHMTRVKDVIDCWFDSGAMPFAQWHYPFENKERFDELFPADFINEGIDQTRGWFY
                     SLIAVSTLVTGKSPYKNVLVNDLVLDKYGKKMSKSRGNTVEPMDLFDRYGVDALRWYL
                     LYVSPVWTPTRFDEDGLKEVLSKFIGTIKNVYTFFALYANTDSIKPQDFFVEYKDRPE
                     LDRWIVSKYNNLVKYVTESMDVYDHNKSVRAIQNFVNEDLSNWYIRRSRRRFWETGLT
                     EDKKAVYNTTYEILVGISQLIAPFAPYLSEEIYRNLTGEVSVHLSYYPKANEELIDEN
                     IEKRMDLVKDIVSLGRASRENAKIKVRQPIQKIHIDGKYQELIDDLVPLIMEELNVKE
                     VVFEHDLNEFMDFTLKPNFKIAGPMLGNNVKAFAGILAKADALEIITKVQSGEKYELD
                     VNGQIIELDDQLLDIRIEAKEGYDVATGNNLFTIIDTNLTEELINEGYAREFISKVQQ
                     MRKNNGYEMMDRIKIFYTSSPEIDKAVKEFEDYIKEETLADLIDISNEEGMEVQNLNG
                     IDTGMKLEKMN"
     misc_binding    complement(8846..9059)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9179..10702
                     /locus_tag="PLB33_06590"
     CDS             9179..10702
                     /locus_tag="PLB33_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOG63227.1"
                     /translation="MEYPRRSYWHDTAEGIKKPAVENADLKNKKFDILIIGAGITGLT
                     TAYLLKDCGFNIGIIEATKSGYGVTGYTTAKITIQHDLFYDYLINSFSMEEAKQYLKA
                     NEEGIRLIKRIINENNIKCDYKEQTAYVYAASVNEVEDLKKELKAYEKLGIDGFYTDT
                     VPIPNKAYGAIGVRNQGQFNPLKYLYSLYNILNQSNCQIYENVRALNIVPHDEHVDVE
                     TESGTIHADKVIVASHYPFDNSFGLYFLRLYQEKEYVIAARTNEPPFEGMYINNNDPT
                     YSLRYQFSDKENLLILAGGNHRVGEKDDENESYNELERFLNSNFKNPQIVSKWSTQDC
                     MTYDKIPYIGRYTDSIDNLYVATGFKKWGMSHSGAAALILKNKILCIEDDYSGIFNPS
                     RFTPVQSSKEFVSSVKSIALGFLNRLEEDLEELSKVNPGEGKIVDYDGKKVGVYRNEA
                     GEYFCINPVCSHLKCALAFNEAEKTWDCPCHGSRFDIKGNILEGPAVLPIDKIKIKK"
     gene            complement(10795..11568)
                     /locus_tag="PLB33_06595"
     CDS             complement(10795..11568)
                     /locus_tag="PLB33_06595"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOG63228.1"
                     /translation="MEIKLKDNERIEDLQCKGLKIIQNKKWFCFGMDAVLLTNYCDIR
                     NNSKIVDLGTGTGIIPILLSGKRNYSKAYGIEIQPEVAEMAERSVKLNSLQEKIEILN
                     IDLKDTLKYLDAGSFDAVISNPPYKLNNSGIINPDDKKAISRHEIKCTLEDVISTASH
                     LLKQYGRFYMVHRPDRLADIICLLRKYRLEPKQIRFVHPKVKAKPNMVLIRASKNGNP
                     ELKFDPPLYIYDDEGNYTEDVHEIYGLENCQLYSCHCYE"
BASE COUNT         3461 a         2202 c         1795 g         4127 t
ORIGIN      
        1 cctttctcac taataagtat cttaaaaagc caatattagt ggttttgtga taataggttt
       61 gactatttcc gaggaaatgc tctaatatat gacataatat tcgtcgaaag tcttcatcta
      121 caatttggca tccatgggta ttaactatgc cgtgaaagct gtttctttta tgcattccca
      181 atactaaagg gccatctttt atttcgtcgc cgaatgaaaa ctccatttta ttcctatatt
      241 cagtttcctt aggacttgga actataggta gaaattcata tttttctgtc aatatttcat
      301 ctaatatatt tttaacctgt ttttctttta tttcaagctg gttcttgtat ggtacagttt
      361 gataggtaca gcccccgcat aagttaaaat gctcacagag cggctctttc tcataatatg
      421 cattttctaa tacttctatt agtcttgcat ctgcactttc ttttcgtgaa cgtacaacct
      481 tagcccttac cttttgtccg ggtaaagtgt tttttattct tattttttta ttttcaaaat
      541 ttgttgttcc tatattagga aactctaaat cattaattgc aacttcaatt atatcgtttt
      601 ttttcatttg tactccttga ttagattctt cactgcgttc agaatgacaa tgtatgagat
      661 tcttcgagct aatggtctca taatgacatg gggacacacc ttctgtcaag ataaaaggaa
      721 tgtccccatt tataataatt tacatttcca taagcttttg tgtcatttct tttattgcag
      781 gaattatttt accgcttggt cttagatttg aataatctcc tacataatga attaatccgt
      841 ttgccggaaa attctgaccg gagactaagt ttttgggaac cggcactgta atcattccca
      901 tattctgagc aaagacagtc attgcattca agcataagtc ccctccgcct tctccgttgg
      961 cacaggttgt aaaggctaac atctttttgc catagaactt ctcactctgc caatagggat
     1021 aaaattcatc catgaatgct ttcatggcac ctgaaacatt cccaaaatat gtgggtgaac
     1081 caagtattat tatatcgctg tcaagaagct tttctacttt tgcttcttct atattcaata
     1141 tttctttttt atattgagtg cctattgtaa attgctttgc aagttcatca aagtttatat
     1201 cctttactct taacaattgc acatctgcat tgtgagcttt gaattcttta taaaattctt
     1261 tcgatatgag aaagttattt ccgcaaatac tgtgaaatac gattgatact attttgctca
     1321 ttcttgtctt ctcctatttt tgcatgttct taagcagctt tcgttttcat ccgcaaacaa
     1381 acagctcaga tactcctcta cctcaagtgc gttttccata ttgagggtac tttttgctat
     1441 taactccata tctgctttat ttaaatttct cactatatat cttgccctga gaatcgaagg
     1501 agaattcatg ctgaattcgt ctagtcccat tcccacgaaa accggtataa gcttggggtc
     1561 tccggctgct tcaccacaca tgccgaccca gatgccggct tcatgtgcat tgtcaattat
     1621 accctttatt agtcttaaaa gtgccgggtg gtattggctg tacaaatatg ataatttgga
     1681 attcatcctg tcaacagcca tggtatattg aatcaaatca ttggttccaa tactgaagaa
     1741 atcaacctcc ctggctaaaa ggtcagatat tattgcagca gaagggattt caatcatgat
     1801 gcctatttct atatcttttt tgaattttat accttctttt gttaattcat cttttgcttg
     1861 ctgcagaatt ttctttgagt cttttaattc cttcatggtg gaaatcatag gaatcattat
     1921 tttaacatta ccgtaaacac tggcccttaa aattgctctc agctgtgttc tgaaaacttc
     1981 cacattgcca agacacagcc ttattgctct gtatcccaag aaagggttca tttcttttgg
     2041 aatatcgata tatggtacat tcttgtcccc gccgatgtcc aatgttctta ttattacagg
     2101 cttatccccc attttaacaa ggacttcctt atattgctca aattgttcgt cttcagtagg
     2161 ttgatgttct ctattcataa atataaattc gcttctgaat agtcctattc cttctgcatc
     2221 attttctaat gccatatcta catcattcgg aacacctata tttgctgtca aagatatttt
     2281 aaatccatct tttgttactg aaggcagtcc tatttgattt tttaattcat ttctaatttc
     2341 atcttctata ttttttttct tagtaaaata aaaaagctgt tttgcattgg gatttaccag
     2401 cactttacct gtcttcccat cgcagattac agtatcgccg ttttttattt tatctgttat
     2461 attgtttaaa cctacaacag tagggatgcc catgattcgt gcaattattg cagcatggga
     2521 tgtcttgcct cctatttccg taatcatggc tgctactttt gttctgtcta tttgtgcagt
     2581 gtcggaggga gtcaaatctt ttgcaacaat gatagagttt tctttcagat tggaaaaatc
     2641 aacacttgaa atacccatca gtattcttat gactctattc attacatctt tgatatcttc
     2701 tgctctttcc ctgaggtatt cgtcttcaat gctttgaaac attttaatat atacattgga
     2761 agtttcattc aaagcatatt cggcatttac accttcttgt tttattctgt tttctacatc
     2821 tgaaataaat gtatcatctt ccaacatcat ttcgtgagat ttaaaaatct gagcttcttt
     2881 ttcacctaat tcatcaattg ttgaaacata caattcgtta agctgctctt ttgctgcctc
     2941 aatggcattc ataagcctgt ctaattcatt tccggcatct ttaatataat tcttggttat
     3001 attaattgat gccttatcaa ttactacggc cttacctata ccaatacccg gtgacactcc
     3061 caatcctttc acaacttcac tacctttcag tcttttaata ctaaatttat attgacaaac
     3121 cttatataaa tttagtaatc tttttattgc attgctccca atttatataa ttatagtact
     3181 atatgcaatg cattgtcaat taaaaatatt gcaatttcaa cattttttgg gacaatgttt
     3241 tcattgttgt ctttctttaa ttcttattat ataatattac aatcgatgag caaagacctt
     3301 gcctttgcac taatgtataa tagtatcagt gtgaagcaag ccaatagtaa cttgcattaa
     3361 tgataaaata aataacaata ataaaatttg gagaatttag gtggagaata aacgaattaa
     3421 aaatataaat ttaaatggta taccaaaaat ttgtgtgccg gtaaccggtg ttacagagaa
     3481 tgaaataatt gatgaattta gcaagctaaa aaatgctccc atagatttgg ttgagcttag
     3541 agcagattat tttgaatttg tacaggacca gacaaaggta acagatattc ttcagcaaat
     3601 aagaaaatta tatgaaaaac ctcttctgtt tacattgcga acaaaaaatg aaggcggggt
     3661 ttcatacata gatgctgact attactttaa actaaacaac agtgtaatcg aaagcaagtt
     3721 ggttgagctt atagatattg aatttttctc tcaagccgaa aaagtagata aaaccgttaa
     3781 attagcaaga aaaaataatg tgatgacaat attgtccaac catgattttt taaaaactcc
     3841 tccggaggaa gaaataatct caagggttaa taagatgatg gattgcggag atattgccaa
     3901 aattgcagtg atgcctaatt ctgaagaaga tgtactgaca ctgctttctg cagcccttaa
     3961 attaaagaaa gaaaaaaaag gccatttcat agccatttct atggggcctc ttggagttat
     4021 ttcaagaata tcatgtgaat taatcggttc ctgcataacc tatgcttctt atggcgataa
     4081 gtcggcaccg ggtcaattag atgtcagact agttaaagag ctgttagaaa tcatgcatgt
     4141 ttagtttatg ggaatatgca atttattgtt gtctgcattt tggtccttaa tcattcccat
     4201 tatatattct tgtatgaagc attcatctaa ttcttcaacg attttaaacc cttcggtttc
     4261 tataagctgc ttcaatgcct catttctgta acctgagaag tattttttgt tcatttcggt
     4321 taccgttttg aatacttctt ctttatgctc gtcgggaatg tccaacagac ctatactatc
     4381 tttatgtgtt ctgcataaat tttcaacgaa aaacttctcg gcatattcat taaatgaaag
     4441 taaattctca tcttcgatat tattttttaa agcatactct tccacattca gcttgattgt
     4501 ttcataatca tagcctcttg acggagaata ggtcaactgt ccgtactggt gagtaaatac
     4561 tggcaggctg cttgtagcta tatcatatat tgtattgcct gtttctacgg atgtatgtgt
     4621 tttaacatct tgaatatgta tatgtcctga cagcacaagc tgtatatcgc attcatggaa
     4681 tgtattaatt acgctggtgt tgttgtccac cgtatagtct tcccatataa tatcgctgtg
     4741 gtcaagcaag ttgtggtgca ttacaactat tatttcggca ttttcttcct ttgccatatc
     4801 tgaacatgat gcaatccatt ttagtgtatc tctgtttata taaccgcccg gttccgggta
     4861 atcctttgtc gtatcaagca ttaacagcca caaattcttg taaggcttaa ctaagtagct
     4921 gagagtattt tcgtctcttg atattgcttc attatatccg tatgatttat atatttccac
     4981 aaactcttct ttgtttatac tttcagttat gcgtatttca ttatctttga agtataatgc
     5041 tttttcatta ttaatatcat ggtttcccgg aactacatat acctttgcac ccgttgattt
     5101 tatacttttt aaacgctctt caaattccaa gtgattaacc ttggagccgt tatttgttaa
     5161 atctcccgga aaaacaacaa tatcgggctg attcttttta acatcatctt ctatgatatc
     5221 aaggaaaaca ctggtatatt tcatcattct atcgagggta ttcaaatagt tgttaaatac
     5281 ttcgctatct tctgtcaatt tttcagacat ataatgaggg tcagcaacta tgtacatctt
     5341 aatactatca tcaatattaa tcataggtac ttcctgaggg atgaggtagg atgcagcaat
     5401 taaaaatatt aaaacaactc ctattatgat aatcttattc ttcattgctc caccctccgg
     5461 ttatataatt ctatttattt cattatacaa taaaatcaaa aaaataacaa tgtttttatt
     5521 ttttagtttt ccaataaaat ggcataaaaa ttggaacact cctctattat ggagtgctcc
     5581 attcctatat taagtatgaa tcagccatac tgtttttgag tattaattca ttttttctaa
     5641 tttcattcct gtatcaatac cgttcaggtt ttgaacttcc ataccttctt catttgatat
     5701 gtcaattaag tctgccagtg tttcttcctt gatataatct tcaaattctt ttacggcctt
     5761 gtcaatttca ggtgagcttg tgtagaatat ttttattctg tccatcatct catatccgtt
     5821 gttctttctc atttgctgta ctttggatat aaattcacgg gcatatcctt cgtttatcaa
     5881 ttcttccgta agattagtat caattatggt aaataaattg tttcctgttg caacatcata
     5941 accttctttt gcctcaattc tgatatccag caattggtca tcaagctcaa ttatttgacc
     6001 gttgacatcc aattcatact tttcgcctga ctgaactttt gtaattattt ctaatgcatc
     6061 tgcctttgcc aaaataccgg caaatgcctt tacattgttt ccaagcatag gtcctgcaat
     6121 cttaaaatta ggtttcaagg tgaaatccat aaattcattt aagtcatgct caaatacaac
     6181 ttccttaaca tttaattctt ccatgattaa tggaaccaaa tcgtcaatta attcctggta
     6241 ttttccgtca atatgaattt tttgaatcgg ttgtctaacc ttaattttag cattttctct
     6301 ggatgctctt cctaagctta caatatcttt taccaaatcc attctctttt caatattttc
     6361 gtctatcagc tcctcgtttg ccttaggata atagcttaaa tggactgata cttcgcctgt
     6421 taaattcctg taaatttctt ctgacaaata aggagcaaat ggagcaatca gttgagaaat
     6481 gccaaccaat atttcatatg tggtgttata aaccgccttt ttatcttcag taagtccagt
     6541 ctcccagaat cttcttcttg aacgtctaat gtaccagttt gacaaatctt cattaacaaa
     6601 attctgaatt gctcttacag atttgttatg gtcataaaca tccatacttt ctgtcacata
     6661 ttttaccagg ttgttatatt ttgatacaat ccatctgtcc agttccggtc tatccttgta
     6721 ttcaacaaag aagtcttgcg gtttgattga atcagtgttt gcatataaag caaagaaagt
     6781 atatacattt ttaattgttc ctataaactt gctcaatacc tcttttaacc cgtcttcatc
     6841 aaaacgagta ggtgtccata cgggcgacac gtacaagagg taccatctta gtgcgtcaac
     6901 accgtatcta tcgaaaaggt ccataggctc tactgtattg cctcttgatt ttgacatttt
     6961 ctttccatac ttatcaagaa ccaagtcgtt taccaataca ttcttatatg gtgacttacc
     7021 cgtaaccagg gtcgatacgg caattaatga ataaaaccat cctctggtct ggtcgatacc
     7081 ttcgttaata aaatcagccg ggaacaattc atcaaatctt tctttgtttt caaacggata
     7141 atgccattgt gcaaaaggca tggcacctga atcgaaccag cagtcgataa cgtcttttac
     7201 tcttgtcata tgctttccgc attcaggaca aacaatatgc acctcatcaa cgtatggtct
     7261 gtgaagctca attgattcat ctatatcttc aactgctttt tcaactaatt cttttcttga
     7321 accgatactt tcgatatgac cgcattcgca tctccaaatc ggaagaggtg ttccccaata
     7381 acggcttctc gaaatagccc agtcattaac attttcaagc cagttgccaa aacgcttttc
     7441 accgacataa tctggatacc aattaaccgt gttgttattt gcaacaagca aatcctttaa
     7501 ttttgtcatt tgaatatacc agcttggtct tgcatagtaa agcagcggtg tgtggcatct
     7561 ccagcagtgc ggataattat gaagaaccgg ttctttttta tataacttcc cgttttcctt
     7621 caaccatctt aatatcggct ggtctgcatc cattacaaac atttctgacc aaggtgtacc
     7681 tctgaacctt cctgtatcat caaccggatt taaaacaggc aaattgtatt ttaatcccgt
     7741 gttgtaatca tcttcaccaa aagcaggtgc agtgtgaaca atacctgtgc cgtcttcaac
     7801 agtaacataa tctgccaaag ttacaaagaa tgcttttttc cccgcaggaa cttcaacata
     7861 aggcagaagt tgttcatatt caacaaactc taattcactg cctttttttt catccaaaac
     7921 atcatattct tcatttatta ctttatccac taaggttttt gcaagaatat atgtttcacc
     7981 ttttacgtct tctttatctt tggttcttat tttaacatag tcaacatccg gatgtaccgt
     8041 caaggtaaca ttggataata aagtccatgg cgttgtggtc catgcaagaa aatatgcatc
     8101 ttcatccttc tttttaaatt tcgcataggc agttattgtt ttaatttctt catagccttg
     8161 tgctacctcg tgagaagcaa gacctgttcc gcaccttgga caatacggaa gaattttgtg
     8221 tccttcatat attaatccct ccttgaaaac tttgtccaat atccaccatt cggattcaat
     8281 gtagtcattt tcaagagtaa tataaggatt atccaaatca atcaaataag ccatacgctc
     8341 agtcatttca cgccataatg cttcgtaggt gaataccgat tcacgacatt ttttattaaa
     8401 ttcgtcaatt ccgtattctt cgatttcctg tttggtactt aagtttaatt gtttttcaac
     8461 ttcaatttca acaggaagtc cgtgagtgtc ccaccctgct tttcttttaa cctgatatcc
     8521 ttgcatagtc ttgtatctgc agacagagtc tttcaatgtc cttgagataa cgtgatgtat
     8581 tccgggtctc ccgttggctg tgggaggacc ttcataaaaa acaaatgatg gtttgttttt
     8641 tcttgtttca atgctttgtt ccaataggtt tatttcttcc caatatttcg atacttcatg
     8701 ctcattattt ttataatcag cttttgatat atctttaaat aatttcatat ttccctccta
     8761 tatgttaatc gtattatatt ttaattgatg tcaggacagg ctccatcacc aatttgtttg
     8821 ccattaaaaa gtcccatgtc aatagacatg ggacgaattt cgcggtacca cccaaattac
     8881 atatacaata atagcatatg tcactcgaaa atttaacgca tttgtacgta atttcttact
     8941 ggtatttcag aaattaaact cctggatgat cttcgttgca ttcagattta taatctcaca
     9001 ccaatcgatt atatctcttt gaatcctact gcaattactc ttccaatcac tgtttttgat
     9061 attgatttta ttctgtatta tgatatatga aaatatgaac aaagtcaagg aataaattga
     9121 aatatttatg aaacaataac actaatatta ttgaaaaata ttatgaaaag aggttactat
     9181 ggaatatcca agacgttcat attggcatga tacagctgaa ggaataaaaa aaccagctgt
     9241 agaaaatgcg gatttaaaaa acaaaaaatt cgacatttta attattggtg ccgggattac
     9301 gggactgact accgcatatt tattaaaaga ctgcgggttc aacattggaa ttattgaagc
     9361 cacaaaatcc ggatacggcg ttacaggcta tactacagca aaaattacta tacagcacga
     9421 tttgttttac gactatttaa taaacagctt cagcatggaa gaagcaaagc aatatttaaa
     9481 agccaatgaa gaaggaataa gattaataaa aagaattatt aacgaaaaca acattaaatg
     9541 cgactacaaa gaacagaccg catatgttta tgcagcatcc gttaatgaag ttgaagattt
     9601 gaaaaaagag ttaaaagcct atgaaaaatt aggtatagac ggattttata cagatactgt
     9661 tcctattcca aataaagctt acggcgcaat aggcgtaaga aaccaaggtc agttcaatcc
     9721 tttaaaatat ttatacagcc tttacaatat tttaaatcaa agcaattgtc aaatatatga
     9781 aaatgttaga gcattaaaca ttgtacctca cgacgagcat gtagacgtag aaacagaaag
     9841 tggaacaatt catgcagaca aggtaatagt tgcatcacat tatccctttg acaatagctt
     9901 cggactttat tttttgagac tgtatcagga aaaagaatat gttattgcag caagaacaaa
     9961 tgaaccaccc ttcgaaggaa tgtacattaa caataatgac cctacttatt ctttgagata
    10021 ccagttttca gacaaggaga atctgctgat attagctgga ggcaatcatc gcgtcggtga
    10081 aaaggatgat gaaaacgaat catataatga attggaaagg tttttgaaca gcaattttaa
    10141 aaatccgcaa attgtttcaa aatggtctac tcaagactgc atgacctacg ataaaattcc
    10201 gtatataggg agatatacag acagtataga taatttatac gtagcaaccg gatttaaaaa
    10261 atggggaatg tcccattccg gtgctgctgc attaatttta aaaaacaaaa tactctgcat
    10321 tgaagatgat tattcaggta tttttaatcc ttctagattt actcccgttc aatcatcaaa
    10381 agaatttgta tcttcggtga aaagtattgc cttaggattt ttgaacagat tagaagaaga
    10441 tttggaagaa ctttcaaaag ttaaccccgg cgaaggaaaa atagttgact atgacggtaa
    10501 aaaggttgga gtatatagaa atgaagcagg agaatatttt tgcatcaatc ctgtttgctc
    10561 acacttaaaa tgtgcgcttg catttaatga agccgagaaa acatgggatt gcccttgcca
    10621 cggctccagg tttgacataa agggaaatat tttggaaggt cctgccgtgc ttccaattga
    10681 caaaattaaa atcaaaaagt aatgagattc ttcattaatt tctttgtcat cctgagcgta
    10741 agcgaaggat ctcatgagat tcttcgagct aaagctttca gaatgacacg tagcttattc
    10801 atagcaatga catgaatata attgacaatt ctccaaccca tatatctcat ggacatcctc
    10861 cgtataattt ccttcatcat cgtaaatata aagaggaggg tcaaatttta attccggatt
    10921 gccgttttta gatgcacgaa ttagaaccat atttggcttt gctttaactt ttggatgaac
    10981 aaagcgaatt tgtttcggtt ctaaccggta ctttcttaat aggcaaatta tatctgccaa
    11041 cctgtcaggg cgatgaacca tgtagaatct gccgtattgt ttcagtaaat gtgaagcggt
    11101 cgatataaca tcctccaagg tgcatttaat ttcatgtctt gaaattgctt ttttgtcatc
    11161 cggattaatt attccgctgt tattaagttt atagggagga tttgatataa ccgcatcaaa
    11221 agaaccggca tccaagtatt ttaaagtatc ttttaaatca atatttaaaa tttctatttt
    11281 ttcctgaaga gaatttaatt ttacgcttct ttccgccatt tctgcaactt cgggctgaat
    11341 ttcaataccg tatgcttttg aataatttct tttaccgctt aacaggatgg gtattattcc
    11401 ggtacctgta cctaagtcaa caattttgga attatttctt atatcgcagt aatttgtcaa
    11461 aagaacggca tccatgccaa aacaaaacca ttttttgttt tgaatgattt ttaaaccttt
    11521 gcactgcaga tcttctattc tttcattatc tttcagtttg atttccattg taaaccttcc
    11581 atata
//
LOCUS       DAOSHP010000059        11330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4838_length_11330_cov_9.440355, whole genome shotgun sequence.
ACCESSION   DAOSHP010000059 DAOSHP010000000
VERSION     DAOSHP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4838_length_11330_cov_9.440355"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..399
                     /gene="rpsO"
                     /locus_tag="PLB33_06600"
     CDS             136..399
                     /gene="rpsO"
                     /locus_tag="PLB33_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOG63229.1"
                     /translation="MNKEIKTQIIEQYRINEKDTGSPEVQIALLTYRINNLTEHLKEN
                     KKDHHSRRGLLQMVGRRRGFLNYLKDNDIERYRSIVAKLGLRR"
     gene            497..2587
                     /locus_tag="PLB33_06605"
     CDS             497..2587
                     /locus_tag="PLB33_06605"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOG63230.1"
                     /translation="MEEKIFKYILAGRELKVTIGKIAELANGSCIVQYGDTVIMANVA
                     ASKEPREGIDFFPLSVDFEEKLYSVGKIPGGFIKREGRPGEKAILTSRLIDRPLRPLF
                     PKGYRNDVAVTVTVLSVDQDCSPEIAGMIGSSIALSISDIPFEGPTGAVNIGLIDGKF
                     IVNPNSKEREITELDLTVSGTKDAVMMVEAGANEVKEEVMLDAIMFAHEEIKKLCDFI
                     IDITAQVGKEKSAYTCFVCDENIEKEIREYAAPIINEAIIEEDKLKRQENIDNFKAQA
                     KEIFLEKYPENETDIDEALYTIVKEKIRENILLRGIRPDNRGIDEIRKISCEVGILPR
                     THGSGLFTRGQTQVLTVATLGVSSDEQTLDGISEETSKRYMHHYNFPSYSVGEARPSR
                     GPGRREIGHGALAERALEPVLPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLSLMD
                     AGVPIKAPVAGVAMGLMKEGDNVVVLTDIQGLEDFFGDMDFKVAGTREGITAIQMDIK
                     VHGIDKEILSKALQKAYVGRMFILDKMLECIDKPREEVSKYAPKIISMSIDPEKIRDV
                     IGSGGKVINKIIEETGVKIDIEDDGSVLIAAENVENAYKAKRIIEGIVKEIEVDEIIL
                     GKVVRIEPYGAFVELSDNKDGLLHISQIDNKRVDKVEDVLKIGDEVLVKVIGIDEKGK
                     IKLSRKAAMQQVQK"
     assembly_gap    2617..2716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2756..2938
                     /locus_tag="PLB33_06610"
     CDS             2756..2938
                     /locus_tag="PLB33_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63231.1"
                     /translation="MKFFCNVAFIRKLLIIIFLVIIIILLIIGRFRTMRVKMVGKEVW
                     GNTLMHCERDTHHNDG"
     gene            2998..4278
                     /locus_tag="PLB33_06615"
     CDS             2998..4278
                     /locus_tag="PLB33_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOG63232.1"
                     /translation="MIKKHTLDNGLRIVMEKIDYVKSASIGIWVNVGSYNENEETNGL
                     SHFIEHMLFKGTKNRKANEIAEEIDNLGAQMNAYTSKECTSFYVKVLEENINEALDIL
                     SDMFFNSLFSPEEIEKEISVVIEEIKMYEDSPEDIVYDKLSEIIFNDSPMAFNILGTE
                     KNLKTFTSDKVKKYMEENYSPSNTVISIAGSFDEDEFIKLIEEKFKHWENKDVKRLNK
                     NSDYQRKIRGINKDLEQLHICIGNKTFGRHDKNYHPLLVLNNLLGGTASSRIFQEVRE
                     KKGLVYSIYSFVTNYSDTGIFSIYAAMSYEQVEEALRTILKEMASIKNGNIKEAEFNR
                     AKQQIKGNFILSLESTSSRMSAIGRRELLYNEILYPDEIIESINNVKIDDVLDVAKEL
                     FNIDNLSITFAGNLHKHSDIEDKIYKVLGEEYEN"
     gene            4268..4702
                     /gene="dut"
                     /locus_tag="PLB33_06620"
     CDS             4268..4702
                     /gene="dut"
                     /locus_tag="PLB33_06620"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOG63233.1"
                     /translation="MKIKVVKKNPFKLPEYETKGSAGVDLQAYVDAPLILKPMERALV
                     PTGLFIELPEGYEAQVRARSGLAIKHGISLVNGIGTIDSDYRGEIKVILINLGGEDFT
                     INNGDRIAQMVIMKHERADFELVEDLNETERGQGGFGHTGIH"
     gene            4742..4975
                     /locus_tag="PLB33_06625"
     CDS             4742..4975
                     /locus_tag="PLB33_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOG63234.1"
                     /translation="MKLSELYSKEIINVDTGENLGIFGDCDLVIDEKTGEIISFISGG
                     NSPFAFFKEPKRKEILWKNIKKIGSDMIIIEND"
     gene            5057..5842
                     /locus_tag="PLB33_06630"
     CDS             5057..5842
                     /locus_tag="PLB33_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOG63235.1"
                     /translation="MENNKMNIPQQKTQPSLNQAQSSTEPINPFIFSGTPNDNEDGSQ
                     PIENVKQVGSITQVEPVKDICFISIIGEIEGHSSLPPQSKTTKYEHIIPMIIGAEMDP
                     QIKGILLLINTMGGDVEAGLAISELIASITKPTVSIVLGGGHSIGTALAVSADYSFIA
                     PTGTMTIHPIRTSGLVIGVPQTFRYFQKMQERITNFIVSNSHVDKQALLDYMYDTDEI
                     ANDVGTVLNADEVVNIGLIDEIGGFSKAMKKLRELIDNMEEEG"
     gene            5974..8301
                     /locus_tag="PLB33_06635"
     CDS             5974..8301
                     /locus_tag="PLB33_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOG63236.1"
                     /translation="MAKTTTNKTNKTNKKTSINKKSTSSKASGKNILIKEITGIFIIS
                     FAVFMIIVATKEAQTGIIGKSINTFTHSAFGAGAKVLPYFILAVGILRLLNIIILNDN
                     NQIAAIIGFFLCFIIFKAISDADVYAHMADSNDFQELIKASAEFGMRNKGGGVFGNAI
                     TFLFVKLIGKNGTYVALGALIFSSIVLLTNQSIIGILKLIKNYIQKFTNLIYRFIFTE
                     SDESKTKKEKPAKKQQEESIQIYDYMSAVQENKKAEITQLKIEDIQKNENIPEASKDV
                     PIEESKEKFKQKEITDEIITISDVKTDNYKLPSIKLLDPMPLHIRGDKEDLKKDGEKL
                     IETLNNFNIPCNILQINKGPTITRYEIQPAPGIKVSRITALSNDIALALATSEIRMEA
                     PIPGKAAIGIEVPNRAKTSVYLRSMIESREYRNAPTKIPFALGKDIAGKNIIASIDKM
                     PHLLIAGATGSGKSVCINSLIMSILFKAKPDEVKLILIDPKVVELSNYNGIPHLLIPV
                     VTDPRKAANALNWAISEMTNRYKIFAQSSVRDIDSYNEKMVTENKEKMPKIVIIIDEL
                     SDLMSVASAEVEEYITRIAQLARACGMHLVIATQRPSVDVITGVIKANIPSRIAFAVS
                     SHIDSRTILDTGGAEKLLGRGDMLYHPIGLPKPVRIQGTYVSDDEIKRVVDYIKEQQI
                     EIKMFKQNEIIKEIENQTNNEEVDEYLEQAIETVVNDGQASVSYIQRKFRVGYSRAAR
                     IIDQLEERGIVGGHEGSKPRKVLITKEELDKMRGN"
     gene            8309..9655
                     /gene="rimO"
                     /locus_tag="PLB33_06640"
     CDS             8309..9655
                     /gene="rimO"
                     /locus_tag="PLB33_06640"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOG63237.1"
                     /translation="MKKIYMHSLGCSKNLVDSENMLGILSKKGYKVTDYADKADYIII
                     NTCSFINDAQQESVNAILFAADIKNKVNKKTKIIVTGCLSQRFGEELSKEIPEIDIII
                     GTAGFERIDEYINEYEKNKELLIDISLELDIDKELPRNTMTEAWVSYLKIAEGCSNNC
                     TYCVIPKLRGLFKSRRIENLVEEAKVLASKGVKEIIIIAQDTSKYGIDIYGEKKLHEL
                     IRKISEIEGIRWIRIHYLYPEDIYDELINEFKNNKKLLKYFDIPMQHINDRILKRMNR
                     KTSREQITSLIQKIRVEVKDAVIRTSLITGFPGETWDEHEELKNFLKEYRLDRVGIFK
                     YSREEDTPAYKLPGQIDEDVKEQRLNELMEIQQEISYENNLLKIGKTYDVLIEEKDAE
                     NVYVGRTYMDSIEIDGCVYVASNKELRLGEIYKVRINDALEYDLIGDVSDEDSRLV"
     gene            9633..10196
                     /gene="pgsA"
                     /locus_tag="PLB33_06645"
     CDS             9633..10196
                     /gene="pgsA"
                     /locus_tag="PLB33_06645"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOG63238.1"
                     /translation="MKIVDWFKNLNLPNKLTVIRVISIPLFLLFLYISKGILRFLPLL
                     IFAGAAVTDLVDGYIARRDNLVSDFGKFMDPLADKLLTASAFIAFVELGYISAWVVVL
                     IISREFLVSGFRTLAASKGVTIAANAWGKIKTVFQMVLIIAILLNYTGYFGFTNTIIA
                     PLIAITVLLTITSGAVYINENIGVIKN"
     gene            10263..11291
                     /gene="recA"
                     /locus_tag="PLB33_06650"
     CDS             10263..11291
                     /gene="recA"
                     /locus_tag="PLB33_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOG63239.1"
                     /translation="MEKDKALELAIAQIEKQFGKGSIMKLGENAKLNIEAIPTGALPL
                     DIATGIGGVPKGRIIEIFGPESSGKTTVALHIVAEAQKKGGIAAFIDAEHALDPAYAK
                     ALGVTVEDLIISQPDTGEQALEIAEALVRSGAIDVIVVDSVAALVPKAEIDGEMGDSH
                     MGLQARLMSQALRKLAGAISKSNAVAIFINQLREKIGVMFGNPETTTGGRALKFYASM
                     RLDVRRVETLKKGDDVYGNRTRVKVVKNKVAPPFKQAEFDIIYGKGISKEGCILDMAV
                     EAGVVNKAGAWYSYENTRIGQGRENSKDYLLSNPELMDEIEKKVLEKYELGDLKVASD
                     ANDPLSYE"
BASE COUNT         4253 a         1665 c         2330 g         2982 t
ORIGIN      
        1 aaataaagct tcttaaaaaa ttgttgttta caaaaataaa taaatatgtt agactttata
       61 agttccatag tctaagttta tcgactcctc aacgatttgc tcggattatg gtgaaataaa
      121 tatatggagg taaaaatgaa taaggaaatc aaaacacaaa ttattgagca gtacagaatt
      181 aatgaaaaag acaccggttc tcctgaagtt caaatagctt tgttgactta cagaatcaac
      241 aatctgactg aacatttaaa ggagaataaa aaggaccatc attcaagaag aggtctttta
      301 caaatggttg gacgaagaag aggttttctg aactacttaa aagacaatga cattgaaaga
      361 tatagaagca tcgtagcaaa attaggatta agaagataag cagatgaccc aatttgcaac
      421 ttggttcgaa cgcttatact gtaagtgaaa ttaaagcggg ggaacccgct ttatatttta
      481 cgttaaagga ggtaacatgg aggaaaaaat atttaaatac atacttgccg gaagggaatt
      541 aaaagtaaca atcggcaaaa ttgctgaact tgcaaacggc tcttgcattg ttcaatacgg
      601 agacactgta ataatggcaa atgtggcagc ttcaaaagag ccgagagaag gtatcgactt
      661 cttcccgctc agtgtagatt ttgaggagaa actatactct gtaggaaaga taccgggtgg
      721 atttattaaa agagagggaa gacccgggga aaaggcaata ttgacttcac gtttaattga
      781 cagacctttg agaccacttt tcccaaaagg atacagaaat gatgttgcag tgactgttac
      841 tgttttgtcg gttgaccagg actgctcacc tgaaattgca ggtatgattg gttcgtcaat
      901 tgcattatca atatcagata taccgtttga aggtcctacg ggtgctgtaa atataggatt
      961 aattgacggg aagtttattg ttaacccaaa cagcaaggaa agagaaataa cagaacttga
     1021 tttaactgta tcaggaacaa aagacgcagt catgatggtt gaagcaggtg caaatgaagt
     1081 caaagaagaa gtaatgcttg atgcaataat gtttgcacat gaagaaataa aaaaactttg
     1141 tgattttata attgatatta cagcccaagt tggtaaagaa aaatcagctt atacctgctt
     1201 tgtatgcgat gaaaatatcg aaaaggaaat aagagaatat gccgctccca taataaacga
     1261 agcgattatt gaagaagaca aacttaaaag acaagaaaat atcgacaact ttaaagcaca
     1321 agctaaagaa atattcttgg aaaaataccc tgaaaatgaa acggatattg acgaagctct
     1381 gtatacaata gttaaggaaa agataagaga aaatatactt ctaagaggta tcaggcctga
     1441 taacagggga atagacgaaa taaggaaaat atcctgtgaa gtaggaatac ttcctagaac
     1501 acacggctcg ggacttttta caaggggaca gacacaggtc cttactgttg ccacacttgg
     1561 agtatcaagc gacgagcaga cattggacgg tatatccgaa gagacttcaa aacgttatat
     1621 gcatcattac aatttcccat cctattctgt cggtgaagca agaccgtcaa gaggaccggg
     1681 aagaagagaa ataggccacg gagctttggc ggaaagagcc ttagagcctg ttctgccaag
     1741 tgaagaagaa tttccttata ccataagact tgtttccgag gttttaagct ctaacgggtc
     1801 aacttcacaa gcaagcgtgt gcggctcaac attatcattg atggatgcgg gagttccaat
     1861 caaagcgcct gtagccggag ttgcaatggg acttatgaaa gaaggagaca atgtagttgt
     1921 tcttactgat attcaaggtc tggaagattt ctttggagac atggacttta aggtggcagg
     1981 tacaagagaa ggaattacag cgattcaaat ggatataaaa gtgcatggaa tcgataagga
     2041 aatattatca aaagctttgc agaaggctta tgtaggaaga atgttcattc tggataaaat
     2101 gcttgaatgc attgacaagc cgagagaaga agtaagcaag tatgcaccga agattatttc
     2161 aatgagcatt gaccctgaaa aaataagaga tgttataggc tcgggaggaa aagtaatcaa
     2221 caagattatt gaagaaaccg gagttaaaat tgacattgaa gatgacggct ccgttttgat
     2281 agcagccgaa aatgttgaaa atgcatacaa ggctaagaga ataatagaag gaattgttaa
     2341 agaaatcgaa gtagacgaaa taatcttagg aaaagttgta agaatagagc cctatggagc
     2401 ttttgtcgaa ctttctgata ataaggatgg attacttcat atttcacaaa ttgacaacaa
     2461 aagggttgac aaggttgaag atgttttgaa aataggtgac gaagtattag taaaagtaat
     2521 cggcatcgac gaaaaaggca aaatcaaact ttccagaaaa gcagcaatgc agcaagtaca
     2581 aaaataataa aacacttaag ataacaaata tcatatnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnncctc atgaataata catttaagta tagaggggga tttttatgaa
     2761 atttttttgc aatgttgctt tcatacgaaa attattgatt ataatttttc tggtaattat
     2821 aataatttta cttataatag gcagattcag gactatgaga gttaaaatgg taggtaaaga
     2881 ggtatgggga aacaccctaa tgcattgtga aagagatacg caccataatg acgggtaaga
     2941 ctatgaagtg agtccatgaa gaaatataag cattagcatg tagtaagcgg aggaaaaatg
     3001 ataaaaaaac acacattaga taacggctta aggattgtaa tggaaaaaat cgactacgta
     3061 aagtctgcat ccatcggtat atgggttaat gtcggttctt ataatgaaaa tgaagaaaca
     3121 aacggattgt ctcactttat tgagcacatg cttttcaagg gcacaaaaaa tagaaaagca
     3181 aatgaaattg cagaggaaat tgacaattta ggcgctcaga tgaatgcata cacaagcaag
     3241 gaatgcacca gcttttacgt aaaagttttg gaagaaaata taaacgaagc attggatata
     3301 ctatcggata tgttttttaa ctctttgttt tcacctgagg aaatagaaaa agaaatatca
     3361 gttgttattg aagaaattaa aatgtatgaa gattcgcccg aggatattgt atacgataaa
     3421 ttatctgaaa taatttttaa cgacagccct atggcattca atattctagg taccgaaaaa
     3481 aatttaaaaa cttttactag tgataaagta aaaaaatata tggaagaaaa ttattctccg
     3541 tctaatactg taatttcaat tgccggaagt tttgatgagg atgaattcat taagctgatt
     3601 gaggaaaaat ttaaacattg ggaaaataaa gatgtaaaac gtcttaataa aaactcagat
     3661 taccaaagaa aaataagggg tattaataag gatttggaac aactccacat atgcataggc
     3721 aataaaacat ttggaaggca tgacaaaaat tatcatcctc tcttagtatt gaacaatttg
     3781 ttggggggaa cggcgagctc aagaattttt caggaagtaa gagagaaaaa aggtctagta
     3841 tattctattt attcatttgt aaccaattat tccgatacag gtattttcag catttatgca
     3901 gccatgagct acgagcaggt cgaagaagct ttaagaacaa ttctaaagga aatggcaagc
     3961 atcaaaaacg gaaatattaa agaagcagaa tttaacagag caaagcagca aattaaagga
     4021 aattttatat tgagtcttga aagcacatcg agcagaatgt cagctatcgg cagaagagag
     4081 ctcttatata atgaaatcct gtatcctgat gaaataattg aaagcattaa caatgttaaa
     4141 attgatgatg ttttggatgt agcaaaagaa ttgttcaata ttgacaacct gagtattaca
     4201 tttgcgggga atttacataa acattctgat attgaagata aaatttataa agtactggga
     4261 gaagaatatg aaaattaaag tagtaaagaa aaatccattt aaattacctg aatatgaaac
     4321 aaaaggctct gcgggagtag acctccaggc ctatgttgat gcacccttaa tattaaagcc
     4381 gatggaaaga gccttggtgc ccacagggtt atttattgaa cttcccgaag gttacgaagc
     4441 acaagtgaga gctcgaagcg gtttggcaat caaacacggt ataagccttg taaacggtat
     4501 aggaactatc gactcggatt acagaggaga aataaaagta attttaatta atctcggtgg
     4561 cgaagatttt actataaaca acggtgacag aattgcccaa atggtaatta tgaagcatga
     4621 aagggcagat tttgaattag ttgaagactt aaacgaaaca gaaagaggtc agggaggttt
     4681 cggtcataca ggaattcatt aacattataa ttgaataaga aatagaaatg ggggggaata
     4741 tatgaaatta agcgagttat acagcaaaga aattattaat gttgacaccg gagaaaattt
     4801 gggaattttc ggtgactgtg acttggttat agatgaaaaa acaggcgaaa taatcagctt
     4861 tatatccggc ggcaattcac cttttgcatt tttcaaagaa ccaaaaagaa aagaaatact
     4921 gtggaaaaat ataaaaaaaa tcggaagcga catgattatc atagaaaatg actgacaatc
     4981 atgatgtcag tgtatatttt taataaaact tcaaagaata atcaggaata cctgattatt
     5041 tttttatgga ggaactatgg aaaataacaa gatgaatatc ccgcaacaga agacacagcc
     5101 atcacttaac caagctcaat cctcaacaga gccgataaac ccctttatat tcagcggtac
     5161 acctaatgat aatgaagatg gcagccagcc tatagaaaat gttaaacagg ttggaagcat
     5221 aactcaagtg gaaccggtaa aagatatttg ttttataagt ataatcggag aaattgaagg
     5281 acactcttca ttgcctcctc aaagcaaaac aacaaagtat gagcatataa ttcctatgat
     5341 aataggagct gaaatggacc ctcagataaa aggtatttta ttgctgatta atacgatggg
     5401 tggagatgtt gaagccggtc ttgccatttc agaattgata gcaagcataa cgaaacctac
     5461 ggtttcaatt gtattaggag ggggacacag cattggaaca gccttagcag tttctgccga
     5521 ttactctttc attgcaccta caggcacgat gacaattcat ccgatacgta ccagcggctt
     5581 agtaatagga gtacctcaaa ctttcaggta tttccagaaa atgcaggaaa ggattactaa
     5641 ttttatcgta tcaaattctc atgttgacaa acaagcctta ttagattata tgtatgatac
     5701 ggatgaaata gccaatgatg taggaacggt attaaacgct gatgaagttg taaatattgg
     5761 attgattgac gaaataggcg gattcagcaa agcaatgaaa aagctgagag agcttatcga
     5821 caatatggaa gaagagggtt aaaaataagg ggacattgta aataaaaaga gaaatgtccc
     5881 caatcattag gatatcgaca aattaccatt gcggaatgat aatatcctat gatataatta
     5941 aatttacaaa gaaacagggg gaaattgctt taaatggcta aaacaaccac aaataaaact
     6001 aataaaacaa ataaaaaaac aagcataaat aaaaagagta caagctctaa agcatccggc
     6061 aaaaatattt taattaaaga aataaccggt atttttatta tatcatttgc tgttttcatg
     6121 attatcgtag caactaagga agcccaaacc ggtataatcg gcaaaagtat aaataccttt
     6181 actcattccg cattcggggc aggcgctaag gtattgccgt attttatatt agctgtaggg
     6241 attttgagac ttttaaatat tattatattg aatgataata atcaaatagc cgcaattatc
     6301 ggattttttc tttgtttcat aatcttcaag gcaatatccg atgcggatgt atatgctcat
     6361 atggctgaca gcaacgactt tcaggaattg attaaagctt ctgcagagtt cggaatgcgt
     6421 aacaaaggcg gaggagtttt tggaaatgcg attacatttt tatttgtaaa gcttatcggt
     6481 aaaaacggaa catatgtagc cttgggcgca cttatctttt catctatagt tcttcttaca
     6541 aatcaaagta taatcggcat attgaaattg attaaaaatt acatacaaaa atttacaaac
     6601 cttatatata gattcatatt tacggaaagt gacgaaagca agactaaaaa agaaaaacct
     6661 gctaaaaaac agcaagaaga aagcatacaa atttatgatt atatgagtgc tgttcaagaa
     6721 aataaaaaag ctgaaattac tcaattaaaa atagaagata ttcaaaaaaa cgaaaatatt
     6781 cctgaagcat caaaagatgt gccgattgaa gaatctaagg aaaaattcaa gcaaaaagaa
     6841 ataactgatg aaattattac aatatcggat gttaaaacag acaattacaa gttgccgtct
     6901 attaagctat tagaccctat gccgcttcat attaggggtg ataaggaaga tttaaaaaaa
     6961 gacggtgaaa agcttattga aacattaaac aactttaata taccatgtaa tatacttcaa
     7021 ataaacaagg gtccgactat taccaggtat gaaattcaac ctgctccggg aataaaagta
     7081 agccgcataa cagcattatc aaatgatata gcactggctc tcgcaacctc ggaaatacgc
     7141 atggaagcac ccatacccgg caaagcggca attggaatcg aagtacccaa cagagcaaaa
     7201 accagcgtgt atttaagaag tatgattgaa tcaagagagt atagaaatgc gccgacaaaa
     7261 attccatttg cgcttggaaa ggatattgca ggcaagaata taattgcaag tatcgataaa
     7321 atgcctcact tgctaatagc cggagctacg ggctcaggta aaagcgtatg cataaattca
     7381 ctaataatga gtattttatt taaagcaaaa cccgatgaag taaagcttat actaattgac
     7441 ccgaaagttg ttgagctaag caattacaac ggcattccgc atttattgat acctgtggta
     7501 actgacccga gaaaagcagc caatgctctg aactgggcaa tttcggaaat gacaaaccgc
     7561 tacaagattt tcgctcagag cagcgtaaga gatatagact catacaatga aaaaatggta
     7621 acagaaaaca aagagaagat gcctaaaatc gtaataatta tagatgaatt gtcagacctt
     7681 atgtccgtag catcggcaga ggttgaagaa tatattacac gtatagctca gctggcaaga
     7741 gcttgcggta tgcacctcgt tattgctacg caaagacctt ccgtggatgt tatcacagga
     7801 gttataaaag caaatatacc ttcgcgtatt gcatttgccg tatcatcaca tattgattca
     7861 agaactatat tggatacagg cggagctgaa aagctccttg gaagagggga tatgctttat
     7921 cacccgatag gccttccaaa gcccgtaaga atacaaggaa catatgtatc ggatgacgaa
     7981 ataaaaagag ttgttgatta tattaaagaa cagcaaattg aaataaaaat gttcaaacaa
     8041 aacgaaataa tcaaagaaat agaaaaccaa acaaataatg aagaagttga tgaatatttg
     8101 gagcaggcaa tcgagacggt agtaaatgat ggccaggctt cggtatccta cattcaacga
     8161 aaatttagag tagggtattc aagagctgct cgaattattg accagcttga ggaaagaggt
     8221 atcgtggggg gacatgaagg aagtaaaccg aggaaagtcc ttattacaaa agaagaatta
     8281 gacaaaatga gaggaaatta aataataaat gaagaaaatc tacatgcatt cattgggatg
     8341 ttcaaaaaac cttgtcgatt cggaaaatat gctgggcata ctttccaaaa aagggtataa
     8401 ggttacggat tatgcagata aagccgatta tatcataata aacacatgct cctttataaa
     8461 tgatgcacag caggagtcgg taaatgcaat cctttttgca gcagacataa aaaacaaggt
     8521 aaacaaaaaa acaaaaataa tagtcacagg ctgcctatct caaagattcg gagaagaact
     8581 gtcaaaagaa atacctgaaa ttgacattat cattggtaca gccggttttg aaagaattga
     8641 cgaatatatt aatgaatacg aaaagaataa agaattgctt atagatatat cattggagct
     8701 tgatattgac aaggagcttc caagaaatac tatgactgag gcttgggttt cctatttaaa
     8761 aatagctgaa ggctgcagca ataattgtac ctattgcgtg attccgaagc ttagagggct
     8821 ttttaaaagc cgcagaattg aaaatttggt cgaggaagcg aaagttcttg ccagcaaggg
     8881 cgtaaaagaa ataataatta ttgctcagga cacgagcaag tacggaattg atatttacgg
     8941 tgaaaaaaaa cttcatgagc ttattcgaaa gatttcagaa atagaaggaa tccggtggat
     9001 tagaattcat tatttatatc cggaagatat atatgacgag cttataaatg aatttaagaa
     9061 taataaaaag cttcttaagt attttgatat tccaatgcag catatcaatg acagaatcct
     9121 aaaaagaatg aacagaaaaa caagcaggga acaaattaca tctcttatac aaaaaatccg
     9181 agtggaagtt aaagatgccg ttatacgaac atctttaata accggttttc cgggagaaac
     9241 ttgggatgag catgaagaat taaagaattt tttgaaagaa tacaggcttg accgagttgg
     9301 gatttttaaa tattcaaggg aagaagatac accggcatac aagcttccgg gacagataga
     9361 cgaagatgta aaagagcaaa gactaaatga gttaatggaa atccaacaag agatatctta
     9421 cgaaaataat ttattgaaaa tcggtaagac ctatgatgta ttgattgagg agaaggatgc
     9481 ggaaaatgtc tatgtgggga gaacttacat ggattcgatt gaaattgacg gctgtgttta
     9541 tgtagcatca aataaagaat taaggttggg agaaatttat aaagtcagaa ttaatgatgc
     9601 attagaatat gatttgatag gagatgtgag tgatgaagat agtagattgg tttaagaatt
     9661 taaatttacc aaacaaactt actgttataa gagtaatttc aataccttta tttctgttat
     9721 tcttatacat aagcaaagga attttaaggt ttctgccgct gttaatcttt gcaggcgcgg
     9781 ctgttactga tttagtcgac ggatacattg caaggagaga taatttagta tctgactttg
     9841 gaaaatttat ggaccctctt gcagacaagc ttttgacggc ttctgctttc atagcttttg
     9901 ttgagctcgg atatataagt gcatgggttg ttgtattgat tatatcaagg gaatttttgg
     9961 tttccggttt cagaacactg gctgcatcaa aaggtgtcac tattgctgcc aatgcatggg
    10021 gtaaaataaa aacagtgttc cagatggtat taattattgc aattttatta aactatacag
    10081 gatattttgg ttttacaaat actataatag ctcctttaat tgcaataacc gtactcttga
    10141 ccataacttc gggagcagta tatattaatg aaaatattgg tgttataaaa aattaaattg
    10201 ttgacaatac atattattct atgtataatc aaaggtgaac aaatgttcta aaggagtcgg
    10261 atatggaaaa agataaggca cttgagcttg ccattgctca aatagaaaag caattcggta
    10321 aaggttctat aatgaagctt ggagaaaatg cgaaattaaa tatagaagca ataccaaccg
    10381 gtgcactgcc tctcgatata gcaacgggaa tcggcggagt accaaaaggg agaattattg
    10441 aaatatttgg acctgaatca tcaggtaaga caacagtagc attacatata gtagcagaag
    10501 cacaaaaaaa gggaggaata gctgcattta ttgatgcaga gcatgcactt gaccctgctt
    10561 atgcaaaagc tctcggcgtt acggttgaag atttgattat atcacagcct gataccggtg
    10621 agcaagccct tgaaatagct gaagccttag tcagaagcgg tgccatagat gtcatagttg
    10681 tagactctgt agcagcctta gttccaaaag ctgaaataga cggagaaatg ggagattctc
    10741 acatgggact gcaggcaaga ttaatgtcgc aggctttaag aaaattagca ggagctataa
    10801 gcaaatcaaa tgcagtggct atatttatca accagctgcg tgaaaaaatt ggagtaatgt
    10861 ttggcaatcc tgaaaccaca accggcggac gtgctttaaa attttatgct tcaatgagat
    10921 tagacgtaag aagagtagaa accttgaaaa agggtgatga tgtttacggc aacagaacca
    10981 gagttaaagt tgttaaaaac aaagttgcac cgccttttaa gcaagctgaa tttgatatta
    11041 tatacggcaa aggtatatca aaagaaggat gcattcttga tatggctgtt gaagccggag
    11101 ttgtaaataa agccggagca tggtattcct acgaaaatac gagaatcgga caaggcagag
    11161 aaaattcaaa agactacctc ttgtcaaatc cggagcttat ggatgaaata gagaaaaagg
    11221 tacttgaaaa gtacgaactg ggagatttaa aagtagcatc tgatgccaat gacccattat
    11281 catatgaata actttgagaa ctttcgctta ggcactataa gtgccttttt
//
LOCUS       DAOSHP010000060        11311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4849_length_11311_cov_9.371446, whole genome shotgun sequence.
ACCESSION   DAOSHP010000060 DAOSHP010000000
VERSION     DAOSHP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_11311_cov_9.371446"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLB33_06655"
     CDS             complement(<1..405)
                     /locus_tag="PLB33_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63240.1"
                     /translation="MKKRICYLICIVFITLLLSNIFQYYCLSNFHFVVNVSAQEREYP
                     EYTDKIMGFGGYVNNKTIFPIKIREINPIGSRGMEYFATLNTTWGFSEIEQNKMGNYE
                     DIENKVILPFKEYDIGVFHKFNGDYVVNADAYE"
     gene            complement(481..642)
                     /locus_tag="PLB33_06660"
     CDS             complement(481..642)
                     /locus_tag="PLB33_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63241.1"
                     /translation="MFISNTTDFYSTKLTKIDDVTFKIEYYDNSKGEKFEDIFKWDGK
                     RFIIAQSIN"
     gene            complement(987..1769)
                     /locus_tag="PLB33_06665"
     CDS             complement(987..1769)
                     /locus_tag="PLB33_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOG63242.1"
                     /translation="MFDNQNDAVNNLLNKEGMTVKDRYLPIEGYTRVEYEEGSFAEFL
                     RNQKLKPYGEKVLYYDGRQKPAKGIYDSVFDVDIGNRDLHQCADAIMLLRAEYLYSQG
                     LYDKISFNFVSGFKAEYKKWMEGNRIDVQGNNAEYHKTAEPSNTYENFRKYMDMVFAY
                     SGTLSLEKELISVSIDNMSIGDVFIVGGSPGHAVIVVDMAVNSLGEKIFLLAQSYMPA
                     QQTQLLINPMDEDISPWYSLKGKEKLITPEWTFELDTLKSWY"
     gene            complement(1993..3639)
                     /locus_tag="PLB33_06670"
     CDS             complement(1993..3639)
                     /locus_tag="PLB33_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOG63243.1"
                     /translation="MKKSRFKKFVLVGLTCTIISTSAIFALADNFDANKAIRLQINNE
                     WVDAQNLYIENDRAMVPLDGIIEELGLKTESDLATNTVKIYDEELTIILTIGNNTAGV
                     IKVISGEIIEEANNLDVAPKIINNQIFVPIRFVAETFGLQVSWDDTLRAVIIEAASGI
                     SVERPIDFEVLDFQAIMDNDLLKNLYDNNYMTKGIYSLVDGEWMYVLAAAGEKPTGGY
                     DLTIDSITEVTKGTAYIHVTLTSPEEGSMVTQVLTYPTVFVKFNKGDIRDIQWDVTGD
                     VTEPETDEQEKVLIENLVKSFGEKLKMVSLLATEDMVKESIKEYYGEFFSQVLLEKWL
                     SDPAKALGRTTSSPWPERIDIISFEKLSDTEYEVKGNIIEMTSVELTQGGIAASRPAT
                     FIIRNIDGKWLIDDIKIGDYIDLNLLVYDNKEYGFYFTLPASWIGYKVLEDKWEGVSL
                     TESKENQSGPIIYIRHPEWTEKTPRQDIPIMIFTKDQWESLNNGEFSVGAAPVGPSIL
                     GENTSYVFALPARYNYEFLTGFEEVEEILENDSLQAYEVN"
     gene            complement(3882..5324)
                     /locus_tag="PLB33_06675"
     CDS             complement(3882..5324)
                     /locus_tag="PLB33_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63244.1"
                     /translation="MKKIYRTKFNVFIVVLICVFLFNIIVYANSAPIYMPDYPSTGIF
                     SVDENTPIEVLKENLLFDFTEEDDDTYSPKAKVIATYEMQNSSNEDVSVYMAFPFIER
                     FSEFSPDEIIITANDEIIPYDTYLGETINNSMNPFQEAENTDFNIEKIIKSITDKKYT
                     ANNFKENERGKLYRFKVSSADDKINFAVSFDFNHENTKIILNGFNRFERNDNNIRVAS
                     GTREEETREIYVLGEDISFDIKGYTSGNLEEETDKFEYEIITEEIDVETFLKNYAAYY
                     LNKYADNLEEMAKGQISEIQLYNLYAESLDKYLDRNLGVCTEGDIMSETWVPRIITLV
                     YSVNFPQNSKKEISVSYKTNGTMDRRETAEPQFTYDYILNPARNWKGFKNLNIKIITP
                     EEAPYIVNSSIELNKEDNNVYTAYSEELPEEDFSFTLYSKEKVTLSDRIEGYLNRTFG
                     YFTPIVIGFVFLITIIVILVVVKTKKRNKE"
     gene            complement(5506..7569)
                     /locus_tag="PLB33_06680"
     CDS             complement(5506..7569)
                     /locus_tag="PLB33_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63245.1"
                     /translation="MKSKVFLLIVLVVILSSCSFQTDEKVSDKNDLDTIGELNDENTD
                     EELNNVITEEELNSKENHADILDRIINDIKNKLEIYKDIKLADIKKIQDTYYVVVKVN
                     EAEMTDHDSMLWKYDMLPELLLTGNAIDILSMDNYYFVSYRESLQYGTKYTITMYDHY
                     DENYEKVIYEGGFSKFTFSPNNDYMYIQNDNEVIILDKDYEILFKNKLYNNAENIEDG
                     HYVDMQFISWTKDNKKLWVATGYHAYVMTFHLIDIENSTQDTFFTGESYGVSEIALDP
                     DNSCIVYSTKPEFYESSAYEEFNNNQTIVYLYFLNLVTNEKIEISKSVTRGFRPKWVN
                     NKIFEYNHPDGFMRLTFNIDNYYLNSGKENPENEENLWQYVEGITPFVNNKDGNWYYN
                     ETTYKINLKIPSIYHLDSPGVYFYWREDGKGCYISLTNYIYEFDKNTSFVENVKNIYF
                     LNNSYINENIFDNVIIEEGTTDNGYKYVYFADDITDYSNDRVLSYIFVQLDDYAICRL
                     DYALFSEDFGKFDADVIINSITHTLTEGRFRDEFFTVYNDDAVNLVNFKVPYFYIQNY
                     RGEYYYETSEKKGSFYPLDYFIDNKEEITLYIQDTDTEIEYMGEITEGYTKNGYKYSY
                     YVEKETVSYQPYGTEKWEEVTRFITNISVRLDKYSVLKMKYELSEEDFSLFDVNEILD
                     SVQLK"
     gene            8038..8355
                     /locus_tag="PLB33_06685"
     CDS             8038..8355
                     /locus_tag="PLB33_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOG63246.1"
                     /translation="MPREKYQTLTEQMFYILLCLQSECCGVDIMKMVSEMTEQRVTVG
                     PGTLYTLLDNFVSQGFITLTNMENRKKSYIITDKGREMLKKEYDRLHKLTEDYERFMK
                     KVI"
     gene            8356..9738
                     /locus_tag="PLB33_06690"
     CDS             8356..9738
                     /locus_tag="PLB33_06690"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOG63247.1"
                     /translation="MKNTKRLMLMNYSYYKEIEKKLELYAKKGLVLEKMGPYFWTFKK
                     TEPQNLKYTVTYFAEGSVFNPHPTDNQQTYFDYAKAAGWDFVCEYNQMQIFCSSLENP
                     PEFETDEKEKLENIHKCMKKSFVISQLLMLLVFALNLYLRFNILKRNPTDFLSSNIDL
                     ATLLMFISIILYSSYTLINYYMWYNKSKKSVDMGCSIIENFNKTQRYFDIGYLIFLFS
                     LVGYMFIHLLTNTAFGIIALSVVQLPLFALVFWGSITLLKRRKFSANANKIISTSLLI
                     LTGVLYLGFIFYSIPRFNFSERSNKPYTTVGEYRLYSDKIPLTCEDLYGHIDFEYYSY
                     EADEEDTPLLHRSHYSQTRPPMKNSPPELEYSIYEPKFEFVKNIVVSDLTKLNDWSDR
                     KLETLDNAVFSTEKAYAFYYEEANGKLFSGEYILVFTDKIIHIDAETPFTDEQIEIIK
                     SKLLSGNSKV"
     gene            complement(9863..10171)
                     /locus_tag="PLB33_06695"
     CDS             complement(9863..10171)
                     /locus_tag="PLB33_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63248.1"
                     /translation="MKNVILSHDDKVRLYSVPDEVADNLDKYCWEFAANWIWKNPNGA
                     KLLQEIRGQKVAIYGSTDFIDYLNEWIFPQQKSVLIEKLNYYDYELPEKYKDYPQYNF
                     "
     gene            complement(10272..>11311)
                     /locus_tag="PLB33_06700"
     CDS             complement(10272..>11311)
                     /locus_tag="PLB33_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63249.1"
                     /translation="VVKQMGNSIKKEYSSSLLSMSTGRRILGGSPIAFGENNTEGRIK
                     NILNYKNPSLWVLIVVSIVIVIFIIGLITNPKSNEAITWDRRPMIMVNGELYLDTGKE
                     IPVEIDESAIIGEISSSVDQSEKPTEEGQTNFGNIGAKYAHFEDNIVVLINNEWVLFE
                     KEVSTKSEETYVLPINREAVENVVRDKNSNWTITDEQNKEYQTTFVIKDRKDEPGRID
                     AGITVTIDSIGNEEERYLAVQVMYPNDYFVKQIQEEQVQNLPLLFDIASEIYGHVDSK
                     ALYDEFIKHLDGNENYEIKGVQWNHEVNGSHVFVKMTPLNNGIMYRKCAVFIMNDVSY
                     EKFKDGLTLNQ"
BASE COUNT         3560 a         2116 c         1500 g         4135 t
ORIGIN      
        1 ctcatatgca tcggcattta ctacataatc cccattgaat ttatggaaaa cacctatatc
       61 gtattcttta aatggcaaaa ttactttatt ctcaatatct tcatagttac ccattttatt
      121 ttgttcaatt tctgaaaaac cccaagttgt attaagagtt gcaaaatatt ccattcccct
      181 acttcctata ggattaattt ccctgatttt aataggaaaa attgttttat tatttacata
      241 cccgccaaat cccattattt tatcagtata ttcaggatat tccctttctt gtgctgaaac
      301 gttaactaca aaatgaaagt tgcttaaaca atagtattga aatatattac ttaacaaaag
      361 agtaataaaa acaatacata tcaaataaca gattctcttt ttcatatttc ctccatgaat
      421 aattctataa tttgaatgtt aacaatgatg agttgttaaa ataataatgc atatcatatg
      481 ttaatttatt gattgtgcaa taataaatct cttaccgtcc cacttaaaaa tatcttcaaa
      541 tttttcgcct tttgaattat cataatattc tattttaaat gtaacatcat ctattttcgt
      601 aagttttgtt gagtagaaat ccgtagtatt acttataaac aatgaataat gatccttaac
      661 aggtaattct tctaccgtcc catccttcct taatgtaaac agtttatatt ctctgccact
      721 cgaccatttt tagcttcaaa aactgatgtt ctccgttaag cgggaataaa tcattatttg
      781 acagtatcac catttcctta gctttattta aataatagtc atcaatattg aaagttaacc
      841 ttataaaacc atcagggtcg ttgtattcaa ttattttatt actaatccat ttcgggctga
      901 atcctttagt tattgatttt gctatttcaa tcttttcatt tcaattacag ttccctttaa
      961 taaaactaaa gatatttact tatctactaa taccagcttt taagtgtatc aagttcaaaa
     1021 gtccattcag gtgttattaa tttttctttt ccctttaatg aataccatgg actaatatcc
     1081 tcatccatcg gatttataag aagttgagtt tgctgggcgg gcatatagga ctgtgccagt
     1141 aaaaatattt tttcacctaa tgaattaact gccatgtcaa caactattac ggcatggccg
     1201 ggacttccgc caactataaa cacatcgcct atactcatat tatcaatact tacacttatc
     1261 agctcttttt ccaaagaaag agttccggaa tatgcaaaaa ccatatccat atattttctg
     1321 aaattttcat atgtatttga aggctctgca gtcttatgat actctgcatt gtttccctgt
     1381 acatcaattc tgttaccttc catccacttc ttatattctg ccttaaatcc tgatacaaaa
     1441 ttaaagctta ttttatcata tagtccttgt gaataaagat actctgccct taaaagcata
     1501 atagcatccg cacactgatg gaggtctctg ttgccgatat ctacatcaaa tacactatca
     1561 taaattcctt ttgccggctt ttgtctaccg tcataatata aaaccttttc gccatagggt
     1621 ttgagctttt gattcctcaa aaattccgca aagctacctt cttcatattc aacccttgtg
     1681 tatccttcta ttggcaaata tctgtcttta acagtcattc cttccttgtt aaggaggtta
     1741 tttacagcat cgttttgatt atcaaacata ttaaaaggta cttcagtcga atccaatccg
     1801 acatttttgt atcctgttat taacaaacct attaataaac cataaatgca taatcttctt
     1861 atggacacca tcacagtctc tccatattat taaaatagtt tttgccagca atttgggatg
     1921 ctcataaatg tagtttgctc tttgtataat aattatagta tatatgctca tgatttgaaa
     1981 tgctcaaaag aattagttta cttcatatgc ctgaagggag tcattttcca atatctcctc
     2041 tacttcttca aatccagtca aaaattcata attgtatctt gcaggcaagg caaaaacata
     2101 gctcgtattc tcacctaata tgcttgggcc gacaggtgct gcacccacgc tgaattcccc
     2161 gttattgagg gattcccact ggtccttggt aaatatcatg ataggtatat cctgtctggg
     2221 ggttttttcg gtccactccg gatgccttat ataaattatc ggaccggatt ggttttcttt
     2281 tgattctgtt aatgatacac cttcccattt atcttctaaa accttgtatc ctatccagct
     2341 tgctggcaag gtgaaataaa acccgtattc tttattatca tatactaata aattaaggtc
     2401 aatataatcc cctattttta tatcatctat tagccatttt ccatctatat ttcttattat
     2461 aaatgttgca ggtctgctgg cagctatacc cccttgtgtc aattcaacgc tggtcatttc
     2521 aattatattt ccttttactt catactcagt atcagacaat ttttcgaacg atataatatc
     2581 tatacgttcc ggccatgggc tggaagttgt tcgtccgaga gcctttgccg gatcacttag
     2641 ccacttttca agaagtacct gtgaaaaaaa ttccccgtag tattctttta tactttcttt
     2701 taccatgtcc tccgttgcca aaagggaaac cattttcagc ttttctccaa aacttttaac
     2761 taaattttct atcagcactt tttcttgttc atcagtttcc ggttcagtaa catcacctgt
     2821 tacatcccat tgaatatctc ttatgtcacc cttgttgaac tttacaaaaa ctgtgggata
     2881 agttaacacc tgggtcacca tgctgccttc ttcgggagaa gtcaaagtaa catgaatata
     2941 tgccgtaccc tttgtaactt ccgtaatact gtctatggtc aaatcatatc ctccagtcgg
     3001 tttctcccct gcagctgcca atacatacat ccattctccg tcaaccaatg aataaatacc
     3061 ttttgtcata taattattat catataaatt tttcagcaag tcattatcca taattgcctg
     3121 aaaatccaaa acctcgaaat caataggtct ttcaacgcta ataccggatg cggcttctat
     3181 tataaccgcc cttaatgtat catcccaact tacctgcaaa ccaaatgttt cagcaacaaa
     3241 gcgtatggga acaaatattt gattattaat tattttaggt gctacatcca agttattagc
     3301 ttcctcaata atttcaccgc ttataacctt tataaccccg gcagtattgt tacctattgt
     3361 taaaataatt gttaattctt cgtcataaat ttttaccgta tttgttgcta aatcactttc
     3421 agtctttaac cctaattctt ctatgatacc gtctaatgga accattgcac ggtcattttc
     3481 aatataaaga ttttgtgcat ccacccattc attgtttatt tgcaacctta ttgctttgtt
     3541 tgcatcaaaa ttgtccgcta atgcaaatat ggcacttgtg gatatgattg tgcaagttaa
     3601 tcctacaaga acaaatttct taaaccttga ttttttcatg tcttttcctc ctgtaacaaa
     3661 caaattgata tataaataag acgcaaattt atcttatttg ttcctcaaac cttaaatctt
     3721 actcaagcta gaaattttta atttattata gaaatggccg atatggattt catctatatc
     3781 agccaccctt aaatcatatt aattttttag ttgtttttag gattcttgta ttgaaccttg
     3841 tccggtgaga acagtccccg atggtcaaaa aactctttta tttattcttt atttcttttc
     3901 tttgttttaa caacaacaag aataacaata atagtaatta agaatacaaa acctattact
     3961 ataggtgtga aatacccgaa agttctgttt aaatatcctt caattctgtc tgataatgtg
     4021 actttttcct ttgagtaaag cgtgaaggaa aagtcttcct ccggtaattc ttctgaataa
     4081 gcagtataaa cattattatc ctctttattt aattcaatgc tgctgttaac aatataagga
     4141 gcttcctccg gagttatgat tttaatattt aagtttttaa atcctttcca gttccttgcc
     4201 ggatttaaaa tataatcata tgtaaattga ggttctgccg tttcccgcct atccatcgtt
     4261 ccattcgttt tgtagctcac acttatttcc tttttagaat tctggggaaa attcacagaa
     4321 tagactaatg taattatgcg tggcacccag gtttcagaca taatatcacc ttcggtacaa
     4381 actcctaaat tcctgtctaa gtatttatcc aaagactctg cgtaaaggtt atacaactgg
     4441 atttctgata tttgtccctt tgccatctcc tcaaggttat cagcatattt attcaaataa
     4501 taagccgcat aatttttcag gaatgtttct acatctattt cttctgttat tatttcatat
     4561 tcaaatttat cggtttcttc ctcaagattt ccgcttgtat aacctttaat atcaaaacta
     4621 atatcttcac ctaatacata gatctctctt gtctcttctt ctcttgtgcc ggaagcaact
     4681 ctgatattat tatcatttcg ttcaaagcga ttaaaaccat ttaaaataat tttggtattt
     4741 tcatgattaa aatcaaagct cacggcgaaa tttattttgt catctgcaga agatacctta
     4801 aacctgtaaa gcttacctct ttcattttct ttaaaattgt tagcggtata ctttttatct
     4861 gtaatagatt ttataatttt ctcaatatta aagtcagtat tttcagcttc ctgaaaagga
     4921 ttcattgaat tgttaattgt ttcacctaaa taggtatcat agggaataat ttcatcattt
     4981 gcagtgataa taatttcatc aggtgaaaac tcgctgaatc tttcaatgaa aggaaatgcc
     5041 atatatactg aaacatcctc atttgaagaa ttttgcattt cataggttgc aatgaccttg
     5101 gcctttggag aataggtatc gtcatcttct tcggtaaaat caaacagcaa attttccttt
     5161 aacacttcaa tcggagtgtt ttcatctacc gaaaatattc ctgttgaagg atagtccggc
     5221 atataaattg gtgctgaatt agcatataca attatgttaa acaaaaaaac gcaaataagc
     5281 actacaataa atacattaaa tttagtgcga tagatttttt tcatagcttt tcctgccttt
     5341 atataattaa ttttttcctt tattcataac tagctgtcac ctattcagac gaattaaaat
     5401 aagaaatgtt ccccacatgg tactcagccg atacccccta tatcttcttt aaaattcaaa
     5461 ttccttaaaa atatatttag gagccttttg ttatttatat aagcattatt tgagttgtac
     5521 agaatctaaa atttcattta catcaaataa actgaaatct tcttctgata gttcatattt
     5581 catttttagt actgaatatt tatctaatct gacggatata tttgttataa atcgcgtaac
     5641 ttcctcccat ttttctgttc cataaggctg ataagatact gtttcttttt ctacatagta
     5701 tgaatattta tatccatttt ttgtataacc ttctgtaatt tctcccatat attcaatttc
     5761 agtatcggta tcttgaatgt aaagtgtaat ttcttcctta ttatctatga aataatccaa
     5821 aggataaaag ctgcctttct tttcagaagt ttcgtaatag tattcaccgc gataattttg
     5881 gatataaaag tagggaactt taaaattaac taaattgact gcgtcatcat tatagacagt
     5941 gaaaaattca tccctgaaac gtccctctgt taatgtatgg gtaatcgaat taattattac
     6001 atcagcatca aattttccaa aatcctcaga aaacaaagca taatctaatc tgcatattgc
     6061 ataatcatct aactgaacaa atatatagga tagcaccctg tcattggaat aatcagtaat
     6121 atcatccgca aaataaacat atttatagcc attatcagta gttccttctt caattattac
     6181 attatcaaat atgttttcat ttatataaga attattaaga aaatatatat tctttacatt
     6241 ttcaacaaaa gatgtattct tatcaaattc atatatataa tttgtcaatg atatataaca
     6301 gcccttacca tcttccctcc agtaaaaata aaccccggga gagtctaaat gatatatgga
     6361 tggtatttta agattaattt tgtatgtggt ttcattataa taccaattgc catctttatt
     6421 atttacaaaa ggtgttatgc cttctacata ctgccataaa ttttcctcat tttcagggtt
     6481 ctccttaccc gaatttaaat aatagttatc aatattaaag gttaatctca taaatccgtc
     6541 aggatgatta tattcaaata ttttattgtt gacccattta ggtctgaagc ctcttgttac
     6601 cgatttggaa atctcaattt tctcattggt tactaaatta agaaaataca gataaactat
     6661 tgtttgatta ttgttaaatt cttcatatgc gctgctttca taaaattccg gttttgtact
     6721 gtaaacaatg cagctgttat caggatctaa agcaatttct gatactccgt agctttcacc
     6781 ggtaaaaaat gtatcttgag ttgaattttc gatatctatg agatggaatg tcattacata
     6841 agcatgataa cccgttgcaa cccatagttt tttattatct tttgtccagc tgataaattg
     6901 catatctaca tagtgaccat cttctatatt ttcagcattg ttatacaact tatttttaaa
     6961 taaaatttca taatctttat cgagtattat tacctcatta tcattttgaa tatacatgta
     7021 gtcgttgttt gggctgaaag tgaattttga aaaaccccct tcataaataa ctttttcata
     7081 attttcatca tagtggtcat acatggtaat agtgtattta gtgccgtatt gcaagctttc
     7141 acgataactt acgaaataat aattgtccat cgataatata tcaattgcat tacctgtaag
     7201 cagcaattcc ggtaacatgt cgtatttcca caacatagaa tcatggtctg tcatttcagc
     7261 ttcatttact tttacaacta cataataagt atcttgtatt tttttaatgt ctgctaattt
     7321 aatatcttta tatatttcta atttattttt tatatcatta attattctgt ccaaaatatc
     7381 tgcatgattt tctttactat tcagttcttc ttctgtaatt acattattca gctcttcatc
     7441 tgtattttca tcatttaatt ctccaatagt atctaaatca tttttatcac tcaccttttc
     7501 atcggtttga aatgaacaag agcttaatat aacaactaaa actattaata gaaaaacttt
     7561 gcttttcata cttgcctcca tgcaatattc attataaata gacgatttcg ttacccaatt
     7621 tgttccacaa aaatacaata gaagtatcaa gtcactttgt tgcagcagtt gaatattata
     7681 ataaaaccac ataccagatt cctcattaat ttatccgtgc atgaaaataa tactaccttt
     7741 agacgctgtg aatcaattaa aacttattaa taggcacatc cgaattggga taacaatata
     7801 tttggtcact catttttgtt ctcctgagta taatcagaga ttatttctct tctaatatct
     7861 tcttcgtaga attaaaacaa gaaatgttcc ccatatggca gttcagccat acccactatt
     7921 ttaaaattaa atttttaaat atatttatga tcctatataa gctattgaca atatcgatat
     7981 tcgatgtata ctggaaatat gaattattga tattcgatat ttgcggaggt gatctaaatg
     8041 ccgagagaga aatatcaaac attaacagag cagatgttct atatactgtt atgtctgcaa
     8101 agtgagtgtt gtggggtgga tattatgaag atggtatcag aaatgactga acagcgggtg
     8161 acggtaggcc ccggaaccct gtatacactg cttgacaact ttgtatctca aggtttcatc
     8221 acgctaacca atatggaaaa ccgaaaaaag agttacatta ttacagataa gggtcgagaa
     8281 atgctaaaaa aagagtatga ccggcttcac aaacttacag aggattacga aagatttatg
     8341 aaaaaggtga tataaatgaa aaatacaaaa agactcatgc ttatgaatta tagttactac
     8401 aaagagatcg agaaaaagct ggagctctat gcgaaaaaag gacttgtttt agaaaaaatg
     8461 ggaccttatt tctggacatt taagaaaact gaacctcaaa accttaaata taccgtcaca
     8521 tattttgccg agggctcggt ttttaatcct cacccaacag acaatcagca gacctatttt
     8581 gactacgcaa aggcggcagg atgggatttt gtttgcgagt ataatcaaat gcaaatattt
     8641 tgttcttccc ttgagaaccc tccggaattt gaaacggacg agaaagaaaa gcttgaaaat
     8701 attcacaaat gtatgaagaa aagctttgtg atatcacaac ttttaatgct tctagttttt
     8761 gcattaaatc tttatttacg atttaacatc ttgaagcgga atcccacgga ttttctgtca
     8821 agcaatatag atttagctac cttgctaatg tttattagca ttattctcta ttcatcctat
     8881 accctaataa attactatat gtggtacaat aaatccaaaa aatctgttga tatgggctgc
     8941 tctattattg aaaatttcaa taaaacccaa agatattttg atataggcta tcttatattc
     9001 ctgttttcac ttgtcgggta tatgtttatc catctgttaa ctaacaccgc ttttggcata
     9061 attgctcttt ctgtggtgca attaccgctg tttgctttag tattttgggg ttcaattaca
     9121 ttgctgaaaa gacgcaaatt ttctgcaaac gcaaataaaa taatctcaac ttcacttctt
     9181 attctaaccg gtgttttgta tcttggattt atattttatt ctatccctcg gtttaatttc
     9241 tcagaaaggt caaataaacc atacaccacc gttggagaat atcgccttta tagcgacaaa
     9301 atcccactta cttgtgagga tttatatgga catattgatt ttgaatacta ttcttatgaa
     9361 gcagatgaag aagacacccc tctgcttcat agaagccatt attcccagac ccgtccccca
     9421 atgaagaatt ctccaccgga acttgaatat agtatatatg agcctaaatt tgaatttgtc
     9481 aagaatattg ttgtatccga tttaacaaag cttaatgact ggtcagacag gaaacttgaa
     9541 acccttgaca acgctgtttt ttccaccgaa aaagcctatg ctttttatta cgaagaagca
     9601 aatggaaaac tgtttagcgg tgaatatata ttggttttta ctgataaaat cattcatatt
     9661 gatgcagaaa cacctttcac agacgagcaa attgaaataa ttaaaagcaa attacttagt
     9721 gggaacagta aagtataata gattacctgc caacgcttta ggcatttttt tatatttggt
     9781 tgattcgcat aatttgaaca ttacgacata acaactgaaa aaattatgct aactcctctc
     9841 tacaaattgg aatttgtata tctcaaaagt tgtattgtgg atagtcttta tacttttcgg
     9901 gtaattcata atcgtagtaa ttcagttttt caattagtac cgacttctgt tgtggaaata
     9961 tccactcatt caaataatca ataaaatcag ttgacccata aattgctact ttctgccctc
    10021 ttatttcctg caacagtttt gctccatttg ggtttttcca aatccaatta gcagcaaact
    10081 cccaacaata cttgtctaag ttatctgcca cctcatctgg aacagaatat aatcttactt
    10141 tatcatcatg actgagaata acatttttca tatataccct ccaaattcct atttaacgat
    10201 attatatata aataattgtt agttcgtaat tatttactga ttcgtaacat ttgcattatg
    10261 taactaatat tctattgatt tagagttaat ccatccttaa atttttcata agatacatca
    10321 ttcattatga aaacagcaca ctttctatac attatcccat tgttaagtgg tgtcatttta
    10381 acaaacacat ggctgccatt tacttcatga ttccactgca cacctttaat ttcataattt
    10441 tcgttgccat ctaaatgctt tataaattca tcatacaaag cttttgaatc gacatgtcca
    10501 tatatctctg aagcaatgtc aaataataaa ggtaaattct gaacttgctc ttcttgaatt
    10561 tgtttaacaa aataatcatt agggtacata acctgaactg ctaaatatct ttcttcttca
    10621 ttaccgattg aatcaatggt aaccgtgata cctgcatcta tccgtccggg ttcgtctttt
    10681 ctatccttta taacaaatgt agtttgatac tctttgtttt gttcatcagt aatggtccaa
    10741 ttcgaatttt tatcccgtac cacattttct acagcttctc tgttaatggg taacacatat
    10801 gtctcctcag acttagtact aacttctttt tcaaaaagaa cccattcatt attaatcaaa
    10861 acaacaatat tatcttcaaa atgtgcatat tttgcaccta tatttccaaa gtttgtttgg
    10921 ccttcttctg tcggtttttc cgattggtct accgaagaag aaatttctcc gataatagca
    10981 ctttcatcaa tttcaacagg aatttcttta cctgtatcca aatataactc accatttacc
    11041 ataatcattg gtcttctatc ccaggtaatt gcttcatttg atttaggatt ggttattaat
    11101 cctataataa atataacaat aactattgaa acaacgatta aaacccacaa tgatggattt
    11161 ttgtaattaa gaatattttt aatcctgcct tcggtattat tttcgccaaa tgctataggg
    11221 cttcctccta aaattctcct acctgtagac atggagagaa gtgaagatga atattccttt
    11281 tttatactgt ttcccatttg ttttactacc t
//
LOCUS       DAOSHP010000061        11081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_4964_length_11081_cov_8.368674, whole genome shotgun sequence.
ACCESSION   DAOSHP010000061 DAOSHP010000000
VERSION     DAOSHP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4964_length_11081_cov_8.368674"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLB33_06705"
     CDS             <1..180
                     /locus_tag="PLB33_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOG63250.1"
                     /translation="DSLEDLIKKAMANGVKMIACTMSMDVMGITKEELIDGVEFAGVG
                     TYLGDAEESNVNLFI"
     gene            265..2331
                     /locus_tag="PLB33_06710"
     CDS             265..2331
                     /locus_tag="PLB33_06710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG63251.1"
                     /translation="MVSSSLNKGLSIITYSKKHMENVIAQLTNLLGDEISIKGYSIQE
                     GINEEICDSLVLICHKYLKEEALKHITPGANVLVARRSLNHSKLEDILQIPEGEKVLF
                     VDVNKYLAESSISLLVELGINHISMYPYYPGQEFPEYADYSITTGETHLVPDYVKNII
                     DIGYSKIDLTTITEIMIKLGIMDERANLLSSRYLQDFAYSSKKIVETLSDNKHITEQL
                     NTILNVINDGIVGIDGNGEIMFSNNNANKLFNNTEINLNDINNFDIKSFQGKTFDVYR
                     EIHEIKGKKVLLTMMPIIEDNANNGIIITFKDLSEIMEMENIVRVNLFKKGHTAKYRF
                     SDIVGKSKNMSETIEIAKKLSRSNSTLLLTGESGTGKELFAHSIHNASKQQYGPFVAA
                     NFAALTESLLESELFGYEEGAFTGARKGGHAGLFEQAHKGTIFLDEIGDASPRIQARL
                     LRVLQEKEVLRVGGTKIIPIDVRVIASTNKDLHKLVQKGDFREDLYYRLNVLPINIPP
                     LRERKSDIPLLIKMFLKKFPNGSEMVISNEVMAKFLEYDWPGNVRELENVIEYLYNLA
                     GSKIELKDLPLDLAGGKKQEDIQTQNQQILEKTLKDLEMEIPLQDVLTILNAIKRSKD
                     EGYAYIGRKKLAELVKDEFTEQMIRSRLEILSQAGLIITERGPKGSRLSLKGEKFLDL
                     INNTLE"
     gene            complement(2421..3044)
                     /locus_tag="PLB33_06715"
     CDS             complement(2421..3044)
                     /locus_tag="PLB33_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63252.1"
                     /translation="MNEINDLRLRDWTRDFLVQRIFGRGRNLMTAENAIIENINTADR
                     TVYVTISYGVLGDFNVINMELVRLVVDRDTVILDQFGRPMSSRELREGMRVDAVFSAA
                     MTRSIPPQARAYSIIVIDDGMQSSFTEDTVIEVDLANRFLYTGIPFDILSQMRFVVTD
                     STTIRDRRGRRIRLRDLRPGDFVRIEHANFMTMSIPPQTTAYDIQVL"
     gene            complement(3122..3280)
                     /locus_tag="PLB33_06720"
     CDS             complement(3122..3280)
                     /locus_tag="PLB33_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOG63253.1"
                     /translation="MNFADGVILFIIAAIVAAVIKVIKKQNKNNGGCSGCSGCSKANS
                     CPSAKKLS"
     gene            complement(3283..5295)
                     /gene="feoB"
                     /locus_tag="PLB33_06725"
     CDS             complement(3283..5295)
                     /gene="feoB"
                     /locus_tag="PLB33_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOG63254.1"
                     /translation="MSVIALAGNQNSGKTTLYNKLTGSNQHVGNFPGVTIEKKEGQLK
                     KHKDISIVDLPGIYSLSPYTMEEVVTRDFLLKEKPDLILNIVDATNIERNLYLTLQLM
                     ELSIPMVIALNMMDEVTASGNSIDVQKLSSHLGIPVVPISASKGEGISELNDVVIRSI
                     KEKPIPKRHDFCVGEVHKAIHSISHLIADQAEKAGYPPRFAATKLVEGDEPTFKSLNI
                     TENQMHIIEHVVKDMEEALKTDREAALADMRYCYIEKICSESVIKKQETKEQQRSEKI
                     DEILTHKYFGIPIFFGIMLFIFWMTFNVIGAPLQEFLEVVIALLTETISNALINIGVS
                     DWVYSLVINGIFVGVGSVLSFLPIIVVLFFFLSLLEDSGYMARVAFLMDKLLRKIGLS
                     GRSFVPMLIGFGCSVPAIMATRTLASERDRKMTIVLTPFMSCSAKLPIYGMITAAFFP
                     DNAALVMIGLYVMGIAVAVLSGLLLKNTIFKGEPVPFVMELPAYRIPSRKSVLIHMWE
                     KAKDFIQRAFTIIFLASIVIWFLQGFNWSFEMVEDSSKSILAYLGSMISPIFRPLGFN
                     DWRASTALITGLTAKEAVVSTLSVLTGAATDAQLLEALFIIFTPISAFAFLTFVLLYM
                     PCVAAFAATKRELGSLKYAVLTALYQTVTAYTAGAIVYQIGSFIGG"
     gene            complement(5292..5525)
                     /locus_tag="PLB33_06730"
     CDS             complement(5292..5525)
                     /locus_tag="PLB33_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOG63255.1"
                     /translation="MTLDKIKPGQGGVIASIGGQGLLRRRLLDMGLTPNTCVKVRKVA
                     PMGDPIELSLRSYILTIRKDEAAKIELKEVFDI"
     gene            complement(5753..6106)
                     /locus_tag="PLB33_06735"
     CDS             complement(5753..6106)
                     /locus_tag="PLB33_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63256.1"
                     /translation="MFALVLILNDIYKLDEINELFYNEELGATSIDSQGIGKVLLDHN
                     INLPILDSVKKLIDGRKPYSKIIFSVIKDREKLDVVIKKLREELDYFEEPGTGFMFVI
                     PVIEVYSHSTENTEF"
     gene            complement(6173..6847)
                     /locus_tag="PLB33_06740"
     CDS             complement(6173..6847)
                     /locus_tag="PLB33_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOG63257.1"
                     /translation="MKLTFLGHSALLIEEGNFKGLVDPFITGNSLSKISVDDLVNITH
                     IFVTHGHGDHIGDTINIAKRNKALIITNHEISAYLSKFKLRTHAMHIGGRTKFDFGTV
                     KMTNALHGSGISENGNMIYGGNPGGFIIEVNGKKIYHAGDTGLTYDMKLLEDEKIDVA
                     FLPIGGNYTMDIEDALKAVDFIKPKTVVPMHYNTFGIINADPEIFKSKASSNVIILNV
                     NESMNV"
     gene            complement(6956..7675)
                     /locus_tag="PLB33_06745"
     CDS             complement(6956..7675)
                     /locus_tag="PLB33_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63258.1"
                     /translation="MLSVKNLNVFYGGIHALKGVTIDVQEGQIVSIIGSNGAGKSTLI
                     NAISGIVKYKDGEIIYKDEKLPKQAHKIVKAGICQVPEGRVIFANLTVMENLNMGAFL
                     RNDKDGIQKDLERIFKLFPILKERQKQMAGTLSGGEQQMLAMGRGLMSNPELILLDEP
                     SLGLAPLLINTIFDIIIEIKKMGKTILLVEQNAFKALSVADKAYVLEQGAIVMEGPAS
                     DLIKNERIQEAYLGKKKSDVG"
     gene            complement(7668..8441)
                     /locus_tag="PLB33_06750"
     CDS             complement(7668..8441)
                     /locus_tag="PLB33_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63259.1"
                     /translation="MSVLSLNNLTKKFGGLTAVDNVTFNIEEGEIFGLIGPNGAGKTT
                     IFNLITGIYSITSGEIFYYDKKIENLKPFQIANMGITRTFQNIRLFKKLTAYDNVLTA
                     CHKLADYNLFDSVIRFRKFKPQEKFINEKTAELLKLMGLWDYKDIVASNLPYGLQRKL
                     EIARALALEPKLLLLDEPAAGMNPEETIQLMGLIKEIRDEFKLTVLVIEHHMDLVMGV
                     CDRIFVLNFGIPLALGTPKEIQKDKRVIEAYLGKEEGHA"
     gene            complement(8438..9340)
                     /locus_tag="PLB33_06755"
     CDS             complement(8438..9340)
                     /locus_tag="PLB33_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG63260.1"
                     /translation="MSWGYLSGILILTGINLMTVLGLSLLTGFTGLFSFGHAGFMAIG
                     AYTAAMFTLKFNILPESMFQLQFYLALIAGGLVAMIFSLIIGKLTLNLKGDYFCIATL
                     GFGEAIRLILDNVDYFGGARGLSSITTQGTTTLTNVVIINIIAITVLVFIIKSRHGRN
                     MVAIREEELAAQTIGIDVFKYKMISFAISAFYAGIAGGLMAHYFGYIQPIMFKLVKST
                     ELTIIVIFGGLGSVSGSVVGTILLTFLPELLRSFANWRLVLYGAAVIIIMISRPQGLM
                     GGKEFSIKGLVRFIAKRRKEGVKS"
     gene            complement(9351..10232)
                     /locus_tag="PLB33_06760"
     CDS             complement(9351..10232)
                     /locus_tag="PLB33_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOG63261.1"
                     /translation="MSYFLQQLINGLSLGSVYALMSVGYSLVYSIMNFSNFAHGGVIM
                     IGAYIGYFCLTLLGVPFFTAFILSAVGSGILAVSMERIVYRPLRKRNAPFLYFIISAM
                     GASIFLENIVIASPIGPTFRSYPAVFSTSPIMIGGIALGRIDLLMFIISTLSLIGLMY
                     IIEKTKIGLSIRATSYSIKASTLMGVNVDKVIFIIFLLGGLLAGLAGMLFGMKYIVYP
                     QIGNITTKSFIAAVFGGLGSLPGAIIGSILLGLIETMTSGYLSSEYRDLIAFVLLIGV
                     LIFKPTGLMGKTTEDKA"
     gene            complement(10273..>11081)
                     /locus_tag="PLB33_06765"
     CDS             complement(10273..>11081)
                     /locus_tag="PLB33_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63262.1"
                     /translation="VLEEYKVPDISTVGTNPKVTVNDDGSVKEFNFRVCFIDPYQGAV
                     AAGYAYDRLGLRNAAVLYDVASDYSQGFTEFFEKTFIEKGGKIVAKEGFKEGDVEFKP
                     QLSKIKDANPDMILMPYYYKEVALSANQARELGIDAVLIGGDGWPSEQLMTMAADAIE
                     GSYIVNHLDFNDPEVKPLQEKYTAKYNLPMELNGYMAHDAFLLLKAAIEDAGVADPVK
                     IAESLTKVTVQGVTGTIKLSPEDHNPIGKEAAIVRVENADYIFQEKYGLQ"
BASE COUNT         3560 a         2231 c         1717 g         3573 t
ORIGIN      
        1 gattcattgg aagatttgat taaaaaggca atggctaacg gtgtaaaaat gattgcctgc
       61 accatgtcaa tggatgtaat gggcattaca aaagaagaat taatagatgg tgttgaattt
      121 gcaggagtag gcacttattt gggtgatgcc gaagaatcca atgtaaacct gtttatctga
      181 tactcatgta atgacgactt aaatgttgaa gaagatatac agttatgata taatttattt
      241 aatgactcat tgatgaggag gttcatggtg tcatcttctt taaacaaagg attatctatt
      301 ataacttatt cgaaaaaaca catggagaat gtaattgccc aattaactaa tcttcttgga
      361 gatgaaatca gcatcaaagg atattccata caagaaggaa tcaatgaaga aatttgcgat
      421 tccctggttc tgatatgtca taagtatctg aaggaagaag cgttaaagca cattactccc
      481 ggtgcaaatg tattggtagc cagaagatca cttaatcatt ctaagttaga ggatatttta
      541 caaatacctg aaggggaaaa agtgcttttt gttgacgtca acaaatattt ggcggaaagc
      601 tccatatctc tgcttgtaga actgggtatt aaccatatat cgatgtatcc ctattatccg
      661 ggtcaagaat ttccggagta tgccgactat tctataacaa ccggtgaaac tcacttagtg
      721 cccgattatg tcaaaaatat aattgatatc ggttatagca aaatagacct taccacaatc
      781 acagaaataa tgattaagct cggaattatg gatgaaagag caaatctatt gtcttcaaga
      841 tatttgcagg actttgcgta ttccagcaaa aaaatcgtag aaacactcag tgataataaa
      901 catattacgg agcagttaaa cacgatatta aatgtaataa atgacggtat agtaggtatt
      961 gacggtaatg gtgaaataat gttcagcaat aataatgcaa acaaactctt caacaatacc
     1021 gaaattaatc taaacgatat aaataacttt gatattaagt catttcaggg taagactttt
     1081 gatgtgtacc gtgaaattca tgagataaaa ggtaaaaaag ttcttctcac aatgatgccg
     1141 attattgaag ataatgctaa taatggtata ataatcactt ttaaggactt gtctgaaatt
     1201 atggagatgg aaaatatagt cagagtcaac ctcttcaaaa aaggacatac tgcaaaatac
     1261 cgttttagcg atattgtggg caagagtaaa aatatgagcg agacaataga aattgctaaa
     1321 aagctttcaa gaagcaattc aaccctcctc ttgaccggag aaagcggtac aggcaaggaa
     1381 ttatttgccc attcaattca taatgcttct aaacagcagt atggtccttt tgttgcagct
     1441 aactttgctg cacttaccga aagcttgctt gaaagtgaac ttttcggtta tgaagaagga
     1501 gcctttaccg gtgcaagaaa gggaggccat gcaggacttt ttgaacaggc acataaagga
     1561 acgatatttc tggatgaaat cggtgatgcc tcacccagaa tacaagcacg attgcttaga
     1621 gtacttcagg aaaaagaggt tcttcgtgtc ggaggaacca agattattcc aatagatgtg
     1681 agggttatcg cttcaactaa taaagacctt cataaacttg tacaaaaagg tgatttcaga
     1741 gaagacttat attatcggct taatgttctt ccaataaata ttcctccttt aagagagaga
     1801 aaaagcgata ttcccttgct gataaaaatg tttcttaaaa agtttccaaa tggaagtgag
     1861 atggtaataa gcaatgaggt tatggcgaaa tttttggaat atgactggcc gggtaatgtt
     1921 cgtgaacttg aaaatgtaat tgaatatctt tacaatctgg cgggaagcaa aattgaactt
     1981 aaagatttac ctttggattt agcaggcggg aaaaagcaag aagatattca aactcaaaat
     2041 caacaaatac ttgaaaaaac tcttaaagat ttagagatgg agataccgct tcaagatgtc
     2101 cttaccatac ttaatgctat aaaaaggtca aaagacgagg gatatgctta tataggacgt
     2161 aagaaactgg cagagctagt gaaagatgaa tttactgagc agatgattag aagccgtctt
     2221 gaaatactat cacaggcagg attaataatt accgaaagag gacctaaagg ttcaagatta
     2281 tcgttaaaag gagagaaatt cttagatttg ataaacaaca cgttagaata atccggaatt
     2341 cttgaaaaag ggagcagaac ttctgctccc tttaatctta taattgttag ttttcttcat
     2401 aactcatgat agttatttat ctaaagaact tgtatatcgt acgcagttgt ttgaggcggt
     2461 atgctcattg tcatgaaatt tgcatgttca attcttacaa aatcgcccgg tcttaaatct
     2521 cttaatctta ttcttcttcc tctcctatct ctgatggttg ttgaatccgt tactacaaac
     2581 ctcatttggc tcaatatatc aaacggaatc cctgtataca aaaatctatt tgctaaatca
     2641 acttctatga ctgtatcttc agtaaaactt gattgcatac catcatcaat aacaattatg
     2701 ctgtaggctc tcgcctgagg aggtatactt ctcgtcatag cagccgaaaa tacagcatcc
     2761 accctcattc cttctcttaa ttctctgctt gacataggac gaccaaattg atccaatata
     2821 acagtatctc tgtcaacaac caatctgact aactccatgt ttattacatt aaaatctccc
     2881 aatacaccat atgatatcgt tacatagact gttctgtcag ctgtatttat attttcaata
     2941 atagcgtttt ctgctgtcat taaatttctg cctcttccga aaattctttg tactaaaaaa
     3001 tctctggtcc aatctcttaa tcttagatca tttatttcat tcatattttc acctcgcata
     3061 tatatattat tatatatatt cagaagatat aaatatgtta ttgataaaaa aactgtctca
     3121 atcaggacag tttttttgca gaaggacagc tgtttgcttt gctgcatccg gagcagcccg
     3181 agcagccgcc attattttta ttttgttttt ttataacttt gattactgct gcaacaattg
     3241 ctgcaataat aaaaagaatt actccatcgg caaaattcat tatcagcctc ctataaagct
     3301 tcctatttga taaacaatag ctcctgctgt atatgcggta accgtctgat ataaggcagt
     3361 caatacagca tactttaacg agcccaactc cctttttgtt gccgcaaaag cagcaacaca
     3421 gggcatgtac aataaaacaa atgttaaaaa tgcaaatgcg ctaataggcg taaatattat
     3481 aaacaaagct tctaaaagct gtgcgtctgt tgcggctccc gtcaatacgc tcaaagtact
     3541 tacaacagct tccttggctg ttaatcctgt aataagtgcc gttgaagctc tccagtcatt
     3601 aaatcccaat ggtctaaaaa tcggtgaaat catggagcct aaataagcaa gtatgctttt
     3661 tgagctgtct tcaaccattt caaaggacca gttaaatcct tgcaaaaacc atatcactat
     3721 ggaagccaag aatattattg taaatgctct ttgaataaag tccttagcct tttcccacat
     3781 atgaatcaga acactttttc ttgaaggtat tctatatgca ggcagttcca ttacaaatgg
     3841 caccggctct cccttaaata tagtattttt caataacaaa cccgataata cggcaactgc
     3901 aatgcccatt acatacaagc caatcataac caatgcagca ttatcaggaa agaatgctgc
     3961 agtaatcatt ccataaatag gtagcttggc actgcaagac ataaaaggcg ttaaaacaat
     4021 tgtcattttt ctgtcccttt cgcttgccaa ggtccttgtt gccataattg caggaacact
     4081 gcatccaaag ccgataagca ttgggacaaa agaccttccc gacaacccga tttttcttag
     4141 aagcttatcc atcaaaaatg ctactcttgc catatagccg ctgtcttcca aaagggacaa
     4201 aaaaaagaat aagactacaa taatgggaag gaaggacaat acacttccaa cgccgacaaa
     4261 tataccgtta attacaagtg aatataccca atcgctaacc cctatattaa tcaatgcatt
     4321 tgatattgtt tcagttaata aagcaattac aacttccaaa aattcctgaa gaggagcccc
     4381 tataacatta aatgtcatcc aaaaaataaa aagcattatc ccaaagaata taggtatacc
     4441 aaaatattta tgtgtcaata tttcatcgat tttttcggag cgctgctgct cttttgtttc
     4501 ctgcttttta ataactgact ccgagcaaat cttttctata tagcaatatc tcatgtctgc
     4561 taaggctgct tctctgtcgg tttttaaagc ttcctccata tctttaacta catgctctat
     4621 aatatgcatt tgattttcag taatattcaa gctcttaaaa gtaggctcat ctccttcaac
     4681 aagcttagtt gcggcaaacc tcggaggata acctgctttt tctgcctggt cagctattaa
     4741 gtgtgaaatg gaatgtattg ctttatgcac ttcacctaca caaaaatcat gccttttagg
     4801 aattggtttc tccttaattg accttatgac aacatcattc agctcagata ttccttcacc
     4861 tttgcttgca gatatgggaa ctacaggtat tcccaaatga gaggataatt tttgtacatc
     4921 tatggaattc ccgctggctg taacttcatc catcatattc agtgcaatga ccataggaat
     4981 tgataactcc ataagctgga gtgtcaagta taaatttctt tcaatatttg ttgcatcaac
     5041 tatatttaat attaaatcag gtttctcctt aagtaaaaaa tccctggtaa caacttcttc
     5101 cattgtatat ggggacaaag aataaatccc cggcaaatcc actatggaaa tatccttgtg
     5161 ttttttcaat tgaccttctt ttttttctat tgtcacaccg ggaaaattac cgacatgttg
     5221 attgcttcct gtcagtttgt tgtataaagt agtttttccg gaattctgat ttcctgcaag
     5281 agcaataaca ctcatatgtc aaatacctct ttcagctcta tttttgctgc ttcatccttt
     5341 cgtattgtca atatatagct tctcaaagat aattctatgg gatcacccat gggtgcaacc
     5401 ttcctgacct tcacacaggt attaggtgtc aaacccatgt caagaagtct tcttctcaat
     5461 aaaccttgcc cgcctattga tgcaatgacc ccgccttgac ccggttttat tttatctaat
     5521 gtcatatcgc acctccaatt tcgatattga taatcattat catttagata ttttaattat
     5581 acccccattg atagcaattg tcaacaaaaa ttaaaaaaaa tatgacatat acggggacct
     5641 tccttcaaaa attatccgca atccatgcga tatctttaat acatccacga attatcggat
     5701 cttgaaggta tgctcccctt cgataacatc atattcttat tatttattaa ttttaaaatt
     5761 cagtattttc cgtactgtgg ctataaacct ctataacagg tattacaaac ataaagcctg
     5821 tgcccggttc ttcgaaataa tccaattctt cccttaattt tttaataaca acatcaagtt
     5881 tttctctatc ttttattacg ctgaatatta ttttactgta gggttttctg ccgtctatca
     5941 acttttttac gctgtcaagt atcggtaaat ttatattatg gtctaagaga accttgccta
     6001 tcccctgact gtctatagaa gtagctccta attcttcatt ataaaacaat tcatttattt
     6061 catcaagctt ataaatatca tttaatatca acactaaagc aaacatataa cctccctggt
     6121 tctgatgata ttgttattat accatatgag ttttctgcta ttcaacaatc atttacacat
     6181 tcatgctttc attaacattc aatattatta cattcgatga tgctttactc ttaaatattt
     6241 cagggtcagc attaatgatg ccaaatgtgt tataatgcat tggaaccacg gtctttggtt
     6301 ttataaaatc tactgcctta agggcatcct caatatccat ggtataattg ccacctatag
     6361 gcagaaaggc aacatctatt ttttcatcct ccaaaagttt catatcatat gtaagccctg
     6421 tatcaccagc atggtaaatc tttttaccgt ttacctcaat aataaatcct cccggattgc
     6481 ctccgtaaat catatttccg ttttcggaaa taccggagcc gtgcagagca tttgtcattt
     6541 ttaccgttcc aaaatcaaac tttgtcctgc cgccgatatg cattgcatga gttcttaatt
     6601 taaacttgct caaataagca gaaatttcgt gattagtaat tatcaatgct ttatttcttt
     6661 tcgctatatt aattgtatct cctatatgat caccatgccc atgagttaca aatatatggg
     6721 ttatattcac taaatcatca acacttattt tactcagaga atttcctgtt ataaaggggt
     6781 ccacaagtcc cttgaaattt ccttcctcaa ttaataatgc cgaatgtccc aagaaagtaa
     6841 gcttcatata atcacctctt ttttattatt aagtatataa tatctttata ttataataaa
     6901 atcaactaaa aaaagaaatc tcacatgaga tttccaaaaa tgacacttaa tttggctaac
     6961 cgacatctga ttttttcttt cctaaatatg cttcctgtat tctttcattt ttaattaaat
     7021 ctgaagcagg tccttccatt actattgcac cttgctccaa aacatatgcc ttatcggcaa
     7081 cagacaatgc cttaaatgca ttttgctcta ccaaaagaat ggtttttccc atctttttga
     7141 tttcaattat aatatcaaat atagtattta tcaataaagg tgcaagccct aatgaaggtt
     7201 catcaagaag gatgagctca ggatttgaca taagacctct tcccattgca agcatctgct
     7261 gctctcctcc cgaaagagtt cctgccattt gtttttgtct ttcctttaat atggggaata
     7321 gtttaaatat tctttccaaa tccttttgaa taccgtcttt atcatttctt aaaaaagcac
     7381 ccatatttaa attttccatt acggtgagat ttgcaaaaat aacccttcct tcaggaactt
     7441 gacaaattcc tgcttttact attttatgag cctgtttagg aagcttttca tccttgtaaa
     7501 taatttcacc atccttatat tttacaatcc cgctgattgc attgataagg gtagactttc
     7561 ctgctccgtt agagcctata atcgaaacta tctgaccttc ctgaacatct atggtgactc
     7621 ccttgagagc atgaattccg ccataaaaaa catttagatt tttaacacta agcatgaccc
     7681 tcctccttcc ctagataagc ttcaataact ctcttatctt tttgaatttc cttcggagtc
     7741 ccgagagcca aaggtatgcc gaaattaagg acaaatatcc tatcgcaaac acccattacc
     7801 agatccatat gatgctcaat tacgagcaca gtaagcttaa attcgtctct tatttcctta
     7861 atcagtccca tcagctgtat tgtttcttca ggattcatac ctgctgcggg ctcatcaagc
     7921 aataaaagct taggctccaa tgccaaggct cttgcaatct caagctttct ttgcagacca
     7981 tatggaagat ttgatgcaac aatatctttg tagtcccata aacccattaa cttcaacagc
     8041 tcagctgtct tttcatttat gaatttttcc tgaggtttaa attttctaaa ccttattaca
     8101 gaatcgaaaa gattataatc ggccagttta tgacatgcag taagcacatt atcataagca
     8161 gtcagctttt taaaaagtct gatgttttga aatgtccttg taattcccat atttgctatt
     8221 tgaaaaggtt taagattttc aatctttttg tcgtaataga aaatttctcc gcttgtaatg
     8281 ctgtaaattc ctgttattaa attaaagatg gtagttttgc cggcaccgtt ggggcctatt
     8341 aaaccaaaaa tctctccttc ttcaatatta aaggtcacat tgtctacagc agttaaccct
     8401 ccaaatttct ttgttaaatt attaagagaa agtacactca tgatttaacc ccctctttac
     8461 gtctttttgc aataaatctt actagtcctt taatactgaa ttcttttcct cccataagtc
     8521 cctgtggcct tgaaatcatg ataataatta cggcagctcc atacagaaca agcctccagt
     8581 ttgcaaagct tcgtaaaagt tccggtaaaa atgtaagcaa aatggttcct actacacttc
     8641 cggatacact tcccaaacct ccaaaaatta ctattatggt caattcggtt gacttaacca
     8701 gcttgaacat aataggctga atgtatccga agtaatgtgc catcaatcct cctgcaatac
     8761 ctgcataaaa tgcacttatg gcaaaggata tcattttata tttaaaaaca tctattccta
     8821 tagtctgggc agctaattct tcttctctta tggcaaccat atttcttcca tggcgggact
     8881 ttataataaa taccaatacc gttatggcaa ttatattaat tatcacaaca ttggtcaatg
     8941 ttgttgtccc ttgtgtagtt atactcgaaa gtcctcttgc acctccaaaa taatcaacat
     9001 tgtcaagaat aagtcttata gcttctccaa aacccaaggt tgcaatgcaa aaataatcac
     9061 cctttaaatt taaggttaac tttccaataa tcaaactaaa aatcatggca accagtccgc
     9121 ctgctatcaa tgccaaatag aattgcaatt ggaacatgga ttccggtaaa atgttaaatt
     9181 tcaatgtaaa catagcagct gtatatgcac cgattgccat gaatccggca tgtccaaaag
     9241 aaaatagacc cgtaaatccc gtaagcaatg ataaacctaa tacagtcatt aaatttattc
     9301 ctgttaatat caaaattcct gataaataac cccagctcat actctacctc ctaagcctta
     9361 tcttccgttg tttttcccat taatcccgta ggcttaaaaa ttagtacgcc tataagaagt
     9421 acaaatgcaa tcaaatctct atattctgaa gatagataac cggaagtcat tgtttctata
     9481 agtccaagga gtatggagcc gattatggca cccggcaaac tgcctaatcc tccgaaaaca
     9541 gcagctatga atgatttcgt tgtaatattg ccaatttggg gatatactat atatttcatt
     9601 ccaaaaagca tgcccgccag acctgccaat aaaccgccaa gcaaaaatat tatgaatata
     9661 accttatcca cattaactcc catcaaagta ctggctttta tactgtaaga tgttgccctg
     9721 attgacaatc caatcttggt tttttctatt atatacataa gacctattaa gcttagggta
     9781 gatattatga acattaataa gtcaattcga cccaatgcaa ttcctccaat cattatggga
     9841 gaagtagaaa aaacagcggg ataagatctg aatgtaggtc ctataggtga tgcaattact
     9901 atgttctcca aaaaaattga agcacccatt gcagatatta taaaataaag aaatggtgca
     9961 tttctttttc taagtggtct gtaaacaatc ctttccatgc ttacagccaa tattcctgag
    10021 ccgactgcgg ataatataaa ggcagtaaaa aaaggcaccc ccagcaacgt aagacagaaa
    10081 tatccaatat atgctccaat cataataacc ccgccatgag caaagttaga aaaattcata
    10141 atactatata caagagagta tcctacagac attaaggcat atacgctacc taaggacaaa
    10201 ccgttaatta attgctgtaa aaaatagctc atattacctc ctcaatgata ggcgggttta
    10261 acccgcctat ttttattgta atccatactt ttcttggaat atataatctg cattttcaac
    10321 tcttactatt gcagcttctt ttccaatcgg attgtgatct tcaggactca atttaattgt
    10381 tcctgttaca ccctgcacag ttacctttgt caaagattca gcaattttaa ccggatcagc
    10441 aactccggca tcctctattg ctgcttttaa cagaaggaat gcatcgtgag ccatataacc
    10501 gtttaattcc ataggtagat tatattttgc tgtatatttt tcttgaagcg gttttacctc
    10561 tggatcattg aaatccaagt gattcactat atagcttcct tcaatggcat cagcagccat
    10621 ggtcattaat tgctctgaag gccatccgtc tccacctatt aagactgcat caattcccaa
    10681 ttctcttgct tgatttgcac tcaatgcaac ttctttataa tagtaaggca tcaatatcat
    10741 atcaggattt gcatccttta ttttactcaa ctgaggtttg aattcaacat cgccttcttt
    10801 aaaaccttct tttgctacaa ttttcccgcc tttttctata aaggtttttt caaagaattc
    10861 tgtaaatcct tgtgaataat cacttgccac gtcatataaa actgctgcat tcctaagtcc
    10921 caatctatca tatgcatagc ctgcagctac cgcaccctgg tatgggtcta tgaaacatac
    10981 tctaaagttg aattctttta ctgagccgtc atcattaacg gttacttttg ggtttgttcc
    11041 aaccgtagaa atgtcaggaa ccttgtactc ttcaagaaca g
//
LOCUS       DAOSHP010000062        10959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_5028_length_10959_cov_9.322542, whole genome shotgun sequence.
ACCESSION   DAOSHP010000062 DAOSHP010000000
VERSION     DAOSHP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10959
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_10959_cov_9.322542"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLB33_06770"
     CDS             <1..1025
                     /locus_tag="PLB33_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008298798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG63263.1"
                     /translation="HLIKFLKEHTKLEIVDRGRWFYAVYKSAHGKKNIAFRADFDAVP
                     IDEYIDIPHGSKIKGVSHKCGHDGHSASLAGFALEIDRYGADNNVYFLFQHAEETGDG
                     AKEAAVFITENNIDEIYAYHNMSGIPFKAVGVKNGVSFCASKGMIIHMEGIPSHASMP
                     EKGRNPSFAIANIINSVPEFTSPDNYKGMVLCTVIQVAIGERAFGTSASKGELLLTIR
                     ALYEEEMNMLQKKIEELAKNEAKKYGLKVNFEYVDVFPETYNHGENVDKIRKAAKNIG
                     YELIEMKEAIRSSEDFGYYTKSTKGAIFLIGNGEDYPAVHINEFDFRDELIEVAADLF
                     KEILLH"
     gene            1094..1441
                     /locus_tag="PLB33_06775"
     CDS             1094..1441
                     /locus_tag="PLB33_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HOG63264.1"
                     /translation="MNKRFISLIVLLILAIVIVAVINHFILNNGETINKVSFNATVLE
                     NYGNSILVNPLEGSPELNSSDKIVVRVPVDNAALKDLSEFTVGSKVKITYDGMIMESY
                     PAQINAYNVEFAK"
     gene            1501..2052
                     /locus_tag="PLB33_06780"
     CDS             1501..2052
                     /locus_tag="PLB33_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63265.1"
                     /translation="MIFNEKTVTLKNGKVCVLRSPKASDAEEMLEYLKKTAAETDFLL
                     RYPEEVVMTIEEEIDFINFVRDNPRGIMICAFIDDKLVGTTHLMGLGDRFKISHRASF
                     GIAVLKEAWHLGIGNALITEMLKIAEDTGFELVELEVASTNHNAIELYKKFGFKIYGT
                     RENGFKFKDGTYCDEHLMLRRIK"
     gene            2146..3084
                     /locus_tag="PLB33_06785"
     CDS             2146..3084
                     /locus_tag="PLB33_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63266.1"
                     /translation="MYSELNKKLEEVQQKVYRLNKIDSILAELRNDAAELRQKVYELK
                     AILDKENLDVEKLDNTNVVSIFYSIIGKLEDKKEKEQREALSARLKYEQASYDLLSIE
                     AEISKLTQEQECYKGCINEFNSLYEQKKELLIKSCEASANKILELTNKINESKRNLKE
                     LKEAISAGQSAVYSLDSALGSLNSAQGWGTWDLLGGGLIADLAKHSHIDNAMREAEQA
                     HNELRRFKSELADVRINYDISFEMDGFAKFADFFFDGLIADWYMQSKINSSYESVSNV
                     KNQVTSTIYKLQQMESLEKQYLEKLENELKNIIIIS"
     gene            3102..3335
                     /locus_tag="PLB33_06790"
     CDS             3102..3335
                     /locus_tag="PLB33_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOG63267.1"
                     /translation="MDGGNNKDKPPKKYNEVFRALADFTQIGVSIAASVLIGVLLGQY
                     LDDKFGTEPWFLSVFSILGVALGIRTLFNIAKK"
     gene            complement(3452..4042)
                     /locus_tag="PLB33_06795"
     CDS             complement(3452..4042)
                     /locus_tag="PLB33_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HOG63268.1"
                     /translation="MNNHLLFKQNQQLAGAVNINSNLMNYTVNVPISLMKSIEGKYFV
                     GTAPNLRFGNATNAWARLYNPPNSGVNLFVNTWTVSDIFTTPYSVQIWFNSTPPGFIQ
                     FSQSVTPSNLTIIPQPQSHIQLQYAISVSGLPTGGIKAYGRYGLAGTTINSEEEGKFI
                     FPPGGSYLIFLSNPERPTLLATGNIAFGWWEEPIMQ"
     gene            4198..4956
                     /locus_tag="PLB33_06800"
     CDS             4198..4956
                     /locus_tag="PLB33_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOG63269.1"
                     /translation="MDNKNYKERIESNRTFMKSAFTEKGYKSDQQKQLPQPPLAKQSV
                     NENKIKLTKDFSSVIKEANILTIINERKSHRVFKNDSITLEQLSFLLWVTQGVKGIRG
                     DNYATERTVPSGGARHPFEVYIAVINVEGLEKGIYHYLSLSHELELIRLLENIENSVV
                     ESLDNQKWAGKSAAVFYYTAIPYRSEWRYTISAHKLVLLDAGHAVQNLYIGCGAIGCG
                     TCAAADYNQELADKLLQVDGEDEFVVYIAPLGLI"
     gene            4989..5507
                     /locus_tag="PLB33_06805"
     CDS             4989..5507
                     /locus_tag="PLB33_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HOG63270.1"
                     /translation="MKITIIGYSSSGKSTLAAKLGNIYNCPVLYLDRVHFKENWIERD
                     DETDKAMVKEFMDHNDNWVIDGNYTNLLREQRLKEADMIIFMCFSRLDCLRQAINRYK
                     TYKGKTRESMADNCSEKLDIEFIKWILLDGRSKEKKEQYAETIRKYNQKIVILRNKKE
                     TASFLEKITHRK"
     gene            5745..6824
                     /locus_tag="PLB33_06810"
     CDS             5745..6824
                     /locus_tag="PLB33_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG63271.1"
                     /translation="MADILILTASFGMGHKSVSNALKEQIEAEYKHVKVSIEDVLEIV
                     NPKVKKFSSKIYSELTESYPSIYNKLYDVKAKNKNNIIDNVLSSLYYKRFYEYMEIEK
                     PKVIISTFPLCSCIVAKVKEEYNDDVNLITVITDVVDSWEWIYEGTNKYLVPTDEIKD
                     RLIAKGVNEENILVTGIPVKKEFLDKKICFSTDKKNTILVVLSGIDSVSKKLLKKLDE
                     YTEYKIKIVTGRNEKLYGKLSDMEYSNLEIYGYVNNLDEMMDESVFIVTKPGGVTVFE
                     AINKEVPLIVLNSIIGQEIGNIEFIKKNKIGIVINDLDDLLYILDYNINNPDTSDKYV
                     KNIKRIKKTLNYSGLSIDSLFDGVI"
     gene            7039..8655
                     /locus_tag="PLB33_06815"
     CDS             7039..8655
                     /locus_tag="PLB33_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63272.1"
                     /translation="MKKIIFLSVMILTLTFVTAGCSAIKEPLDISTSTNKEPPDISIF
                     IDNKEIEYVCAKNKWDGSVFDREDTFVTIFKEQKDIPIFINGSIAEISFKNNPPDAFQ
                     IMDILIDEESRQIYTDKEIINIPVELINGKCSFKIEKHLASSLSSYYEPGKKDIRGFR
                     MIASWGENECEYAFIIKTYEIDRTEYLTGEIITDGDYEILSSGIGCICFVPDKESREI
                     IEDKYSSYDLYYLNDESYFLYYDDISVTEGLPDKLGIYKVKVKFDLKEIYNYDRFDLI
                     DINLVDNIGTVLYEGKEYETNNLDLDVKVKDKVCGLIVESVDKFEGGGFRVGFCGEIE
                     TEGYYNISYSEMNACNYGTIYYDDKYESNVPMMMGESNNKQLFLFQDKEELFSQLEEH
                     SSFGRGKFKISNFHIVYNVGMGREPTEILTEIVSLDERYKNMFEIIDKAETNIKGHNG
                     IFAIVSNTAEYDENNYAKSYDYYYINKNNPKKVYLLTTEEYYFLDEVVSETEFSLITE
                     GYNQATESYGVLNRINCKITDKGVELSYDK"
     gene            8716..9234
                     /locus_tag="PLB33_06820"
     CDS             8716..9234
                     /locus_tag="PLB33_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63273.1"
                     /translation="MNKKIILVILAILLVITGFFIREITQKDIELIFNNKDAIYSIGL
                     NQSYINKYTKELVEEGNADSLDTLLTMISYSRDVDSYGRLSRFIANTNNDYSILFIEF
                     KGLNGEYLDFLKESDVVQTRKFTLDEIVYIKEYLKISEEITGIIDSLIKDELYETDRT
                     VELITQIRKLRN"
     gene            9272..9655
                     /locus_tag="PLB33_06825"
     CDS             9272..9655
                     /locus_tag="PLB33_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63274.1"
                     /translation="MNFYKIIIYLFMLLPIFHLILIGMILRKSSGKISAKLISIFKIL
                     TVEIIIFLLSINVPIPDGGIMFYFIIALVIGIPVLIYQFKYYKNFLYDNRQFKMTISY
                     SIWVIAVIIGLIYWFAYYIANPLLG"
     gene            9843..10208
                     /locus_tag="PLB33_06830"
     CDS             9843..10208
                     /locus_tag="PLB33_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOG63275.1"
                     /translation="MNTYKLTESEEKFADLIWMKEPIGSGELVKICEKEMNWKKSTTY
                     TVLKKLCDKGIFKNENALVSSLIKKDEYYSKQSIRFVEDTFGGSLPKFLTSFISGKKL
                     SEHQAEELKRLIDEHKEAD"
     gene            10208..>10959
                     /locus_tag="PLB33_06835"
     CDS             10208..>10959
                     /locus_tag="PLB33_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOG63276.1"
                     /translation="MENLFLTVLNMSLKASYVIIFVIVIRLFLKRFPKVYSYALWFAV
                     LFRLICPFSFDSLFSLIPKSVNIPQDIAYSPKPEINSGIAAIDSAVNNVLPPPLNAAA
                     SANPMQIWIAIGEAIWLIGIAVLIIYSVYTTVKLYRNLRFAKHLEDNIYIINGYKTPF
                     VFGIVNPKIYLPNHLTESEKSYVLLHEQTHIKRLDHIVKLAFFIVTCIHWFNPLVWAA
                     FYLMGEDMELSCDEKVVKQMGNSIKKEYSSSLL"
BASE COUNT         4059 a         1431 c         2134 g         3335 t
ORIGIN      
        1 aacatttgat aaagtttttg aaagaacata caaaattaga aatagtggac agaggcagat
       61 ggttttatgc tgtatataaa tcagcccacg gaaagaaaaa tatagcattt cgtgcagatt
      121 ttgatgcagt cccgatagat gaatatatag atattccaca cgggtctaaa attaaaggtg
      181 tttcgcataa atgcggacat gacgggcatt ctgcttcatt agcaggtttt gcactggaaa
      241 ttgaccgata cggggcggac aataatgtct actttttatt tcagcatgct gaagaaaccg
      301 gagatggagc aaaagaagca gctgtattta taaccgaaaa taatattgat gaaatatatg
      361 cttatcacaa tatgagcggt atacctttta aagctgtcgg cgtgaaaaac ggagttagtt
      421 tttgtgcctc aaagggaatg attatacata tggaaggtat tccttcacac gccagtatgc
      481 cggagaaggg aaggaatcca tcctttgcta ttgcaaatat aataaattca gttcctgaat
      541 ttacatctcc cgataactat aaaggaatgg tcctttgtac agtaatacaa gtagctatag
      601 gggagagagc ttttggtaca agtgcaagca agggtgagct cttgctgact attcgcgctt
      661 tatatgaaga agaaatgaat atgctgcaga agaaaattga ggaactggcg aagaatgaag
      721 ctaaaaagta tggactaaag gtaaactttg aatacgttga tgttttcccc gaaacatata
      781 atcatggtga aaatgtggac aaaataagga aggcagcaaa aaatatagga tatgaattga
      841 tagaaatgaa ggaagcaatc agaagctcgg aagatttcgg gtattacaca aaatcaacca
      901 aaggtgccat ctttttaata ggtaatggag aagattaccc tgctgttcac attaacgaat
      961 ttgatttcag ggatgaactg attgaagttg ctgcagactt atttaaggaa atattgttgc
     1021 attaaaggaa cgtaagtgag tataattgga acatttcttt attaataacg tctatatact
     1081 tgaggtgata aaaatgaaca aaaggtttat atctttgata gtattattaa ttttggcaat
     1141 cgtaatagtt gctgttataa accattttat attaaataac ggtgaaacaa ttaacaaagt
     1201 atcttttaat gcaactgtac ttgaaaatta cggcaattca atactggtaa atccattaga
     1261 aggctcaccg gagcttaatt caagcgataa aattgttgtt agggttcctg tagataatgc
     1321 tgctttaaaa gatttatcgg aattcaccgt tggcagcaaa gtaaaaatta cttatgacgg
     1381 aatgattatg gaaagctatc ctgcacaaat aaatgcctat aacgtcgagt ttgcaaaata
     1441 aaattatatc atcctcccca ttatgtggag gaaatcgatt aaattcgaag gtggttaatt
     1501 atgatattca atgagaaaac tgttacatta aaaaatggga aagtatgtgt attaagaagt
     1561 ccaaaagcat ctgatgcaga ggaaatgctt gagtatttaa agaagacggc agctgagaca
     1621 gactttttgt tgcgttatcc tgaagaagta gtaatgacaa ttgaagaaga aatagatttt
     1681 ataaattttg taagagataa tccaaggggt ataatgattt gtgcttttat tgacgataag
     1741 cttgtcggaa caacacactt aatgggtctt ggtgacaggt ttaaaatctc tcacagagca
     1801 tctttcggta tagctgtttt gaaggaagca tggcatttag gaataggaaa tgcactaata
     1861 actgaaatgt taaagatagc agaggatacc gggttcgaac tggttgagtt agaggttgca
     1921 agcacaaatc acaatgcaat agagctatat aaaaaatttg gatttaaaat ctacggcaca
     1981 agagaaaacg gttttaaatt taaagacggg acatattgtg atgagcattt gatgctgaga
     2041 aggataaaat aaccgtgtca ttctgaacgt atgtgaagaa ctcatacatt aaagatagaa
     2101 tccttctcta tgctcaggat gataataaat tatctgggag atactatgta ttcagaatta
     2161 aacaagaagt tagaagaagt ccaacaaaaa gtatacagat taaataaaat cgattcaatt
     2221 cttgccgaat taagaaatga tgcggctgaa ttaagacaaa aagtatatga gctgaaggca
     2281 atattggata aggaaaacct tgatgtagag aaattggata atacaaatgt agtatctata
     2341 ttttactcta ttatcggtaa attggaagat aaaaaagaaa aggaacaaag agaagcactg
     2401 tcggcaagac ttaaatacga gcaagcaagt tatgatttgt taagtataga agcagaaatt
     2461 tctaaattaa cacaagagca agagtgctac aaaggctgta tcaatgaatt caatagtctg
     2521 tatgaacaaa aaaaagaatt actgatcaaa tcctgtgaag cttcagctaa taaaatatta
     2581 gagttaacaa ataaaataaa tgagtcaaaa agaaatttaa aagagcttaa ggaggcaatt
     2641 tcagcaggac aatccgcagt atacagcctc gattcagcct tgggcagtct aaattctgct
     2701 cagggttggg gaacatggga cttgttaggg ggcggactta ttgcagactt ggcaaaacat
     2761 tctcatattg ataatgcaat gagagaagcc gaacaagctc ataatgaatt gagaagattt
     2821 aaatctgaac ttgcagatgt aagaatcaac tatgatataa gttttgaaat ggatggtttt
     2881 gccaaatttg cggatttctt ttttgacgga ctcattgcag actggtatat gcaatctaaa
     2941 atcaatagtt cctatgaaag tgtatcgaat gttaaaaatc aggttacaag caccatatat
     3001 aagctgcagc agatggaaag cctggaaaaa caatatttag aaaaattaga aaatgagctg
     3061 aagaatataa ttatcatttc atagaaatgg aggggttatc tttggatggg ggaaacaata
     3121 aggataaacc accaaagaaa tacaatgaag tttttcgtgc tttagctgac tttacgcaga
     3181 taggagtgtc tatagcagca tcagtgttaa tcggcgtatt gttaggacag tatttagacg
     3241 ataaatttgg tactgaacca tggtttcttt cggttttttc tatattgggt gtggcgcttg
     3301 gaatcaggac cttattcaat atagctaaaa aataaaaatg ataaaaaaga gaattctaaa
     3361 attctgggga cggttctttt cgttctcaga atttttatga caaggggacg ggggtactgt
     3421 catcaaattt tttgctgtta tgtttagaca actattgcat aattggttcc tcccaccatc
     3481 cgaatgcaat atttcctgta gcgagcaggg ttggtctctc ggggtttgat aaaaaaatca
     3541 aatatgaacc gccgggagga aatataaatt ttccttcttc ctctgaattt atggtagtac
     3601 cggcaagacc gtatctgccg taagccttga ttccgcctgt tggcaaacca ctgaccgaaa
     3661 tagcatattg gagctgtata tgagactgcg gctgtggaat aattgtcaaa ttggatggag
     3721 taacagattg agaaaattgt ataaatcctg gaggtgtact gttaaaccaa atttgtacac
     3781 tgtatggagt tgtaaagatg tcacttacag tccaagtatt tacaaataaa tttacacccg
     3841 aattcggcgg gttgtacagc cttgcccatg cattggtagc attaccgaat cgcaggtttg
     3901 gagccgtccc gacaaaatat tttccctcaa tagatttcat taagcttatg ggaacattaa
     3961 cagtgtaatt cattaaattc gaattaatat ttacagctcc tgcaagctgt tgattttgtt
     4021 taaataatag atggttattc attagatttc aacctttcat attatagtta tttattatat
     4081 gatataatat atgaaaggtg aatttaccat gtctatctga actttacaaa cgtaatcttt
     4141 aataataaaa taatttagaa aggggaaaaa tggagcaatc cattagataa tggtattatg
     4201 gataacaaaa attataaaga gagaatagaa agcaacagaa cattcatgaa aagtgcattt
     4261 acggaaaaag gatataaatc agaccaacaa aagcagctgc ctcagccgcc tttagctaaa
     4321 caatcagtca atgaaaacaa aataaaactt acaaaagact tttcaagtgt aattaaagaa
     4381 gcaaatattt taacgattat caatgaaaga aaaagccacc gtgtttttaa aaacgatagt
     4441 attacattag agcagctgtc attcttatta tgggttactc aaggcgtaaa gggaataaga
     4501 ggcgataatt atgctacgga aagaactgtt ccgtcaggag gtgccagaca tccatttgaa
     4561 gtttacatag cagttataaa tgtagaaggt ttagaaaaag gaatttatca ttatctgtcg
     4621 ctaagccatg aattagagtt aatcaggtta ttagaaaata tagaaaactc tgtagtcgaa
     4681 agtctcgaca atcaaaaatg ggcagggaaa tcggcagctg tattctatta tactgccata
     4741 ccatatagaa gtgagtggag gtatacaatc agcgcgcata aattagtatt attggatgcc
     4801 ggtcatgcag ttcaaaactt atatattgga tgcggagcaa taggatgcgg aacttgtgcc
     4861 gcagcagatt ataatcagga attagccgat aaattactgc aagtggacgg cgaggatgaa
     4921 tttgtagtat atattgctcc tcttggactt atataagaga catcaaacaa taaatacgag
     4981 gtgttttcat gaagattaca ataattggtt acagcagttc aggcaagtcc accctggcag
     5041 ctaagctggg gaatatttat aattgtccgg ttttatattt ggatagagta cactttaaag
     5101 aaaattggat agaacgggat gatgaaacgg ataaggccat ggtgaaggaa tttatggacc
     5161 acaacgacaa ctgggtcatt gacggcaatt atactaatct tcttagagag caaagattaa
     5221 aagaagcaga tatgataata tttatgtgtt tttcaaggct ggattgcttg aggcaggcaa
     5281 ttaacagata caaaacatat aagggaaaaa ctcgtgaaag catggctgat aattgttctg
     5341 aaaaattgga tatcgagttc attaaatgga ttttactgga tggaagatca aaagaaaaaa
     5401 aagaacagta tgctgaaaca atccgtaaat ataatcaaaa aattgtgata ttaagaaaca
     5461 aaaaagaaac cgcttctttt ttggagaaaa ttacgcatag gaaatgaaat attattatgt
     5521 gttaaggata aaaagctctt cgaggtgtat tttgagccga acgaaccgca aaaatttatg
     5581 tattatgata attttttata aaaaggtaga tccaaattta ccttttttta atgaaaaatt
     5641 aataatattc tatattataa ttaatatata aatctaatac taactatatc attatatgtt
     5701 tatgtattta gaattcaatt acatatgaag aaaggagcat ttatatggct gacattttga
     5761 tattgaccgc atcttttgga atggggcata aaagtgtttc caatgcctta aaagaacaaa
     5821 ttgaagctga atataagcat gtgaaggtga gtattgaaga tgtattggaa atagtaaacc
     5881 ccaaggttaa aaaatttagt tcaaaaatat actcagaatt gacagaaagc tatccatcaa
     5941 tttataacaa gctgtatgac gtaaaggcaa aaaataagaa caatataata gacaatgtgc
     6001 tgagcagctt atattataaa aggttttatg aatatatgga aattgaaaaa ccgaaggtta
     6061 taatcagcac ttttcccttg tgttcatgca ttgttgccaa agttaaggaa gaatataacg
     6121 atgatgtaaa tttaattacg gttataaccg atgtcgtgga cagctgggag tggatttatg
     6181 agggaacaaa caaatatttg gttcccaccg acgagataaa agacaggtta attgccaaag
     6241 gagtcaatga ggaaaatata cttgtaacag gcatacctgt taaaaaagaa tttttggata
     6301 agaaaatatg tttttcaact gacaaaaaga acacaattct tgttgtgtta agcggaattg
     6361 acagtgtttc aaaaaagctc ttaaaaaaac ttgatgaata cacagaatat aaaatcaaaa
     6421 tcgttacggg aagaaacgag aagctatatg gaaaattatc cgacatggag tattctaacc
     6481 ttgaaattta cggatatgtg aataatttgg atgaaatgat ggatgaatcg gtttttattg
     6541 ttacaaagcc cggaggcgta acagtatttg aagcaattaa taaggaagtg cccttgatag
     6601 tactgaattc aatcatcggc caggaaatcg gaaatattga atttatcaag aaaaacaaaa
     6661 ttggtattgt aataaacgac cttgatgatc tgctctatat actggattat aatatcaata
     6721 acccggatac ttccgataaa tacgtaaaaa atataaaaag aatcaaaaag actttgaatt
     6781 acagcggtct ttcaattgat tccttgttcg acggggtaat ttaagtgaaa gataccctac
     6841 tgcttaacat ataaataaag aagatggtgt atccttttgg tttaaattta aataaagtta
     6901 aaactttcag cacagcttaa tgaacaaaac caatataata aacgtctaaa atatatacac
     6961 tatttaccaa gaaagcagga gtccatatat gacgttcttg aagaaaacgt tctcggtttt
     7021 aatataaggg gggatataat gaaaaaaatc atatttctat cagttatgat tttaacattg
     7081 acatttgtta cagcagggtg ttcagctata aaggagccat tagacatatc aacttcaacc
     7141 aataaagaac cgcctgacat ttcaatattt atcgacaaca aagagataga atatgtttgt
     7201 gcaaaaaata aatgggatgg aagtgttttt gacagagagg atacttttgt taccatattt
     7261 aaagaacaaa aagatattcc tatttttata aacggcagca ttgctgaaat ttcatttaaa
     7321 aacaatccgc cggacgcatt tcaaattatg gatattttaa ttgatgagga aagcagacaa
     7381 atttatacag acaaagaaat aataaatatt cctgttgaat taatcaatgg aaaatgctct
     7441 tttaaaatag aaaaacattt agcatcatcc ttaagttcat attatgagcc gggaaaaaag
     7501 gatataagag gatttagaat gattgcttcc tggggagaaa acgagtgtga gtatgccttt
     7561 ataattaaaa catatgaaat agacagaacc gagtatttga caggtgaaat aataactgac
     7621 ggtgattatg aaatactatc aagcggcata ggctgtattt gctttgttcc tgataaggag
     7681 agtagggaaa ttatcgaaga taaatacagt tcttatgacc tttactattt aaatgatgaa
     7741 agttactttt tatattatga cgatatatct gttacagaag gcttgcctga taaattgggg
     7801 atttataagg taaaggtaaa atttgattta aaggaaattt ataattacga tagattcgat
     7861 ttaatcgata taaatttagt tgataatata ggtacagttt tatatgaagg caaagagtat
     7921 gaaacaaata atttggattt ggatgttaag gtcaaagaca aggtttgcgg acttatagta
     7981 gaatcagtag ataaatttga gggtggagga tttagagttg gattttgcgg agaaatagaa
     8041 acagaaggct attacaatat atcttatagt gaaatgaatg catgtaatta tggaacaata
     8101 tattatgatg ataaatacga aagtaatgtt cctatgatga tgggagagag taataataaa
     8161 caattattct tatttcagga taaagaagag ttattcagtc aattagagga gcattcatca
     8221 tttggcagag gtaagtttaa aatttctaat tttcatatag tttataatgt tggtatggga
     8281 agagagccta cagaaatatt gacagaaatt gtatcattag atgaaaggta taagaatatg
     8341 tttgaaatca tagataaggc tgaaacaaat ataaagggcc ataatggcat ctttgcaata
     8401 gtatcaaata cagccgaata tgatgaaaac aattatgcta aatcttatga ctattattat
     8461 ataaataaaa acaatcctaa aaaagtgtat ttactgacta ccgaagagta ttatttctta
     8521 gatgaagttg tcagtgaaac agaattttca ttaataacag aagggtataa ccaagcaaca
     8581 gaatcttacg gcgttttaaa tagaattaat tgcaaaatta ctgacaaggg tgtagagtta
     8641 tcatatgata aatagatata tacaatttga aaatatggaa actttctaat tttcattatt
     8701 gagatggagg agagcatgaa caaaaaaata atcttggtta tattggcaat tttattggtt
     8761 ataaccggtt ttttcataag agagattaca caaaaagata ttgagctaat atttaacaac
     8821 aaagatgcta tctactctat tggtcttaac caaagctata ttaacaagta tacaaaagaa
     8881 ttagttgaag aaggtaatgc tgatagtttg gataccttgc ttacgatgat ttcatattca
     8941 agagatgtgg attcgtatgg taggctctcc agattcattg caaataccaa taatgattac
     9001 tctattttat ttatagagtt caaggggtta aatggagaat atctggattt tttgaaggag
     9061 tctgatgttg tacaaactag aaaatttaca ttagatgaaa ttgtctatat aaaggaatat
     9121 ttgaagataa gtgaagaaat aaccggtatt attgattcct taataaaaga tgaattatac
     9181 gagacggata ggactgtgga attaataaca caaataagaa aattgagaaa ttaattatta
     9241 tacataaggg gataacgaaa gggtgtttaa agtgaatttt tataaaataa ttatctattt
     9301 gtttatgttg ctgccaattt ttcatcttat tttaattgga atgatattaa gaaaaagcag
     9361 tggtaaaatt tcagcaaaac taatttccat ttttaaaata ttaactgttg agattataat
     9421 ctttttactt tccataaatg tacctatacc tgatggtgga ataatgtttt attttattat
     9481 tgcgcttgtt attgggatac cagtattaat ttatcaattc aaatattaca agaactttct
     9541 atatgataac cggcagttta aaatgacaat atcttattca atttgggtaa tagcagttat
     9601 tattggtttg atttactggt ttgcttatta tattgccaat ccattgcttg gatagttgtc
     9661 gtaagttaat ataataatct atctctatgt aatatgtaaa ttgcagccag gctgatattg
     9721 atggaatatc agctatatta ttatatataa cataaaatat ttgattattg ttttgacatt
     9781 ttggtctata tagtatagac ttaacttaag gtcgataaaa tgtagactta aggaggtcgc
     9841 ttatgaatac atataaactt acggaaagcg aagaaaaatt tgctgattta atatggatga
     9901 aggagcctat tggctcaggt gaacttgtga agatatgtga aaaagaaatg aattggaaaa
     9961 agtctactac atatacagtc ttgaaaaaac tgtgcgataa aggaatattt aaaaatgaga
    10021 atgctcttgt ttcatctttg ataaaaaagg atgaatatta ctcaaaacaa agcatacgtt
    10081 tcgttgagga tacctttgga ggctcattgc cgaaatttct gacatctttt attagcggta
    10141 aaaaattaag tgaacatcaa gcagaggaat tgaaaagatt gattgatgag cacaaggagg
    10201 cagactagtg gaaaatttat tcttaaccgt tttaaatatg agcctcaagg caagctatgt
    10261 aattattttt gtaatagtga ttagattatt tttgaaaaga ttccctaagg tttattccta
    10321 tgcattgtgg tttgcagtgc tgttcagact gatatgtcca ttttcctttg atagtttatt
    10381 cagcttaatt cctaaaagtg taaatattcc gcaggatatt gcttactccc caaaacctga
    10441 aattaacagt ggcatagcag caattgacag tgcagtaaac aatgtattgc caccgcccct
    10501 taatgcggct gcaagtgcta accctatgca aatatggatt gcaattggag aagcaatctg
    10561 gcttatcggt atagcggtac ttatcattta cagtgtttat acaactgtaa agctatatcg
    10621 aaatttaagg tttgcaaaac atttagaaga taatatttac ataattaatg gatataaaac
    10681 accatttgtt tttggtatag ttaatccaaa gatttatctt cctaaccatc ttacagaaag
    10741 tgaaaagtcc tatgtattat tacacgaaca gacacatatc aagagattag accacattgt
    10801 aaaattagct ttctttatag ttacatgtat acactggttt aatcctcttg tgtgggctgc
    10861 cttttatctt atgggcgaag atatggaact gtcatgtgat gaaaaggtag taaaacaaat
    10921 gggaaacagt ataaaaaagg aatattcatc ttcacttct
//
LOCUS       DAOSHP010000063        10799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_5139_length_10799_cov_9.449832, whole genome shotgun sequence.
ACCESSION   DAOSHP010000063 DAOSHP010000000
VERSION     DAOSHP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5139_length_10799_cov_9.449832"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..343)
                     /gene="fliE"
                     /locus_tag="PLB33_06840"
     CDS             complement(47..343)
                     /gene="fliE"
                     /locus_tag="PLB33_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOG63277.1"
                     /translation="MFIENIKPINASQINELNKPKEKISELAFKSIFDTAIENYKNAE
                     AEVDKDIYKLSIGETDDLHNLMINVEKAQISLDLVIQLRNKALEAYNEIMRMGV"
     gene            complement(346..777)
                     /gene="flgC"
                     /locus_tag="PLB33_06845"
     CDS             complement(346..777)
                     /gene="flgC"
                     /locus_tag="PLB33_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOG63278.1"
                     /translation="MSFLKTLNISGSGLTAQRARMDVISENIANIDTTRTEEGGPYRR
                     KMVVLKTSGDFKNMMVKNLNQYEPGGVEVAEIMEDNSEFKAVYNPEHPDADENGYVNM
                     PNVDSLKETIDIMEAFRAYQANITALNTVKQMAIKALEIGR"
     gene            complement(846..1229)
                     /gene="flgB"
                     /locus_tag="PLB33_06850"
     CDS             complement(846..1229)
                     /gene="flgB"
                     /locus_tag="PLB33_06850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOG63279.1"
                     /translation="MRVNVNLNLLKKSLDGLWLRQQTTMENIANYSTPGYKSKTVDFE
                     TILSANLKNTYGKTTSEINEQILSGNIIIKENDNLSMRLDGNNVDLEKENLQLAKTQI
                     QYMYTVAEINSYFSKLKSVISEGKK"
     regulatory      complement(1355..1437)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1735..1971)
                     /locus_tag="PLB33_06855"
     CDS             complement(1735..1971)
                     /locus_tag="PLB33_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOG63280.1"
                     /translation="MLVIKRKVSESILIGDDIEIIISEISQDKVKIAISAPQDIKITR
                     KELAETCEFNKIASEKINEISLNDIKNKLKAIKN"
     gene            complement(1965..2417)
                     /gene="fliW"
                     /locus_tag="PLB33_06860"
     CDS             complement(1965..2417)
                     /gene="fliW"
                     /locus_tag="PLB33_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOG63281.1"
                     /translation="MSEKMKIKTRDFGEILVGEEDIINFTTGIYGFDDYKKYIILKDS
                     PEDDIMFLQSLDNMELSFVLIDPYAIFIDYNPILNEDDLCELKSTGTAELKYLVIAII
                     KDDIKESVVNLKSPIAINPNNRTAKQVILQNEYPLRYNIVSTEEGNKC"
     gene            complement(2410..3015)
                     /locus_tag="PLB33_06865"
     CDS             complement(2410..3015)
                     /locus_tag="PLB33_06865"
                     /inference="COORDINATES: protein motif:HMM:NF039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HOG63282.1"
                     /translation="MEPLLEIKTIPMAFEMKINRARLELATTDATFEVSRDKGGLQMQ
                     MKPAKIKIDTVEARYSAGLKSAMHLISDVAKKGVQAAYEATATYAREGNLMLDINIMD
                     NPIPEIAMKKFISDVSFNIGFIPSTGPDISWDTGGISMSFAMDKLDFDWNVEKPQIKF
                     IPGSIEFIISEYPSVEIKYVGTPIFVPPSANPNYKEIDTLV"
     gene            complement(3031..4023)
                     /locus_tag="PLB33_06870"
     CDS             complement(3031..4023)
                     /locus_tag="PLB33_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63283.1"
                     /translation="MRITTSMMSNKYIKNLNKSAYEMNYLSEKVETGRKFVKGSEDPV
                     SAIKAYKLRREYRTTEIYDTNIRDVESFLTAAETNLTEISNNMESVYTSYLKGINGTM
                     NDEDREIIAKQLENLQRSILTSLNAKFEDRYVFGGTNKEEIPFTLDMNGNLLFKGLDV
                     NDPANKEALDKLANETINIDLGLGMTFNGDGELNTDTVFDMSMSGIKFMGYGGTDDNP
                     ENLYNLIGKIKDELRNPDFSIDKISPYIDRFEEQKKQVLVHITDIGAKTNYLDFLKTR
                     NEDNQFNLNKKLLEVEFEDPAEAIVNFKMQEYSYNAALSMGNRILQNSFIDFMK"
     gene            complement(4034..5650)
                     /gene="flgK"
                     /locus_tag="PLB33_06875"
     CDS             complement(4034..5650)
                     /gene="flgK"
                     /locus_tag="PLB33_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOG63284.1"
                     /translation="MLRSTFLGYKTATSALKVNQNHMDVVGQNISNINTKGYTRQRLD
                     ISSVAFNTSNLKYGTPGPVIGQGVNANGVSQFRDAFLDLRYRMETSKAGSEQVQLEAL
                     RDLESVFDEISMDKLDAQFSDLLTQLHSLTSSPSDPVMEGVVRTSAEILTQMFNDYSR
                     DIETIYNQQLNYLENGAVEKVNELTKNIAKLNQQIKESNISGNPALELNDERNMLVDE
                     LSNYIDIEVDIEPLNIGGGKTIDELVIRLSGTTYELVNRNNSNTISVNEQNGLIKIEL
                     TGATDTDDITNFIKGGQIDGYLKFLNGKGEFATVDDIDPVNGYEKAAEVKGIQYYNNM
                     LDTLANKFATVMNELNRKPDSFDIDGNVQTWITKPLFGARGADPSDPDTEIIRAGNIK
                     ISDDWASATDSYITNTIKEKAGVDEDNTGAVDNILRMINAFNAKNEFDVDRDPSTTND
                     ILFKGTFQEFLSYTTTKLNLQTADVQISFDTYSETQFHIDYARSSMSSVDLNEEGVNL
                     LQYSKSYNAAARLMTTLDEMLETLINRMGV"
     gene            complement(5670..6125)
                     /locus_tag="PLB33_06880"
     CDS             complement(5670..6125)
                     /locus_tag="PLB33_06880"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOG63285.1"
                     /translation="MDSLYQLLKEYTEIYKMFLELEYDKYEAVIEDDVIALDNIVSQE
                     EVYYLQMKGIEHKREKLLNSLELSGKTLKEIIDAAENESKIKLQEQFEELNNKIKELN
                     KINDLLKIVIEVRLRRINNKMNNLGEKENIYTNDKSKNVTEGLLISHKI"
     gene            complement(6135..6413)
                     /locus_tag="PLB33_06885"
     CDS             complement(6135..6413)
                     /locus_tag="PLB33_06885"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOG63286.1"
                     /translation="MKINNINNNNYINYKGNSNLIKKEEINKENKYDVIDFNNKNKEN
                     ISLQSIKKKVVSQINEETNADKINRIKDSINNKTYSIDVEEIVRRLLR"
     gene            complement(6484..6963)
                     /locus_tag="PLB33_06890"
     CDS             complement(6484..6963)
                     /locus_tag="PLB33_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG63287.1"
                     /translation="MNNENIMQMREVIPWLIFKLNETLYTVNSKYITSIVIKPDDVTF
                     VPNVAEHIIGLIHLRGNVIPLVDLKLLLNTEDESGNNSANNSKENKAMVVVLEKGNSF
                     IGLVVDEVLSVENIFAYEETEEIKQMCNNGFITGVAKGHKSEEVLLVIDEEKIMSNA"
     gene            complement(6956..7480)
                     /locus_tag="PLB33_06895"
     CDS             complement(6956..7480)
                     /locus_tag="PLB33_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOG63288.1"
                     /translation="MDKFNSLHTYIEEEDAQVNKYLTFYIENQVFGIPIAHVVQITGM
                     QEIEEVPEFPSYAKGIINLRGIIVPIIDVRIRLKKQEIEYNERTCIIVTNIEGLYIGF
                     IVDSVNEVINIYYENISEPPQVGNNYVITYITGIAKLDKKIVILIDLNKLLNEKEIGS
                     LELIKDEIMGDYNE"
     gene            complement(7633..9258)
                     /gene="groL"
                     /locus_tag="PLB33_06900"
     CDS             complement(7633..9258)
                     /gene="groL"
                     /locus_tag="PLB33_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOG63289.1"
                     /translation="MAKIIKYDEDARRAMERGVNALADTVKVTLGPKGRNVVLNKSFG
                     SPQITNDGVTIAREIELSDPYENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVAAGANPMILKKGIEKAVAAAVEELKNITKKVETSEEIAQIASISAGDEEIGKLI
                     SQAMDQVGKDGVITVEESKTMDTELVVVEGMQFDRGYLSAYMVTNTEKMEAVLEDPYI
                     LITDKKITNIQDVLPVLEQIVQTGKSLLIIAEDVEGEALATLVVNKLRGTFNCVAVKA
                     PGFGDRRKEMLRDIAILTGGQVISEELGLELKETTLDMLGNAGTVKIDKENTVIVNGA
                     GSKEEIEERARQIRAQHDESTSEFDREKLMERLAKLTGGVAVINVGAATETEMKEKKL
                     RIEDALNATRAAVEEGLVSGGGVALLTTIDKVNKVAEGLDGDAKTGAKIIEKALEEPI
                     RQIAINAGLEGSVIVEKIKNSEKGVGFDALNEKYVNMIEAGIVDPTKVVRSALQNAAS
                     VAAMLLTTEAAVTDEPKKEEPAMPPMGGGMGMM"
     gene            complement(9274..9555)
                     /locus_tag="PLB33_06905"
     CDS             complement(9274..9555)
                     /locus_tag="PLB33_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOG63290.1"
                     /translation="MTVKPLGERVVIKMIEAEEKTKSGIVLPGSAKEKPQVAEVLAVG
                     AGITNDEKKKDEIKVGDKVIYSKFAGTEVKIDGEELIIIKLADVLAVIE"
     gene            9910..>10799
                     /gene="fba"
                     /locus_tag="PLB33_06910"
     CDS             9910..>10799
                     /gene="fba"
                     /locus_tag="PLB33_06910"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOG63291.1"
                     /translation="MALVTSQEMFKKAYEGKYAVGAFNVNNMEIIQGIVEAAKQENAP
                     LILQVSAGARKYANPIYLRKLVEAAIEDTGLDICLHLDHGEDFEICKQCVDDGFTSVM
                     IDGSKYPFEENIALTKRVVEYAHSKGVVVEAELGKLAGVEDAVKVRTKDATYTDPDEA
                     VEFVERTGVDSLAIAIGTSHGAYKFKGDPTLDFERLETITNLLPGFPLVLHGSSSVPK
                     EFVDLCNKYGGQIPGAAGVPEEMLRRAAQLGVCKINIDTDLRLALTACIRQFFVEHPS
                     EFDPRKYLGPGRTAIKDMVA"
BASE COUNT         2986 a         2087 c         1535 g         4191 t
ORIGIN      
        1 ttttttaatt gttctgacat ttatagctcc taagatagta tgagacctaa acccccatac
       61 gcattatttc attgtaagct tctaaagctt tatttctaag ttgtattacc aggtctaaag
      121 aaatttgtgc tttttcaaca tttatcatta gattgtgcaa atcatccgtt tcacctattg
      181 ataatttgta aatgtcttta tctacttctg cttctgcatt cttataattt tcaattgcag
      241 tatcaaatat tgatttgaat gcgagttcgc ttattttctc tttcggctta tttaattcat
      301 taatttgaga tgcatttatc ggcttaatat tttcaataaa cataattacc tcccgatttc
      361 taatgcttta attgccattt gcttaaccgt gttaagagca gttatgttag cttgatatgc
      421 tctgaatgct tccattatat ctatcgtttc ttttaatgaa tcaacattag gcatattaac
      481 atatccgttt tcatccgcat cgggatgttc gggattgtat actgctttga actcactgtt
      541 atcttccata atttcagcaa cttccactcc acccggctca tactgattta aatttttcac
      601 catcatattt ttaaagtcgc cggaagtttt taatacaacc atttttcttc tgtaaggtcc
      661 tccttcttca gtccttgtag tatcaatgtt tgctatattt tccgaaatta catccatcct
      721 tgccctttgc gcagttaaac cagacccact tatattgagc gtttttaaaa aagacatatt
      781 accctccttg aaatcgttat cttatgtata ttatcattgt gtatctcatg ctaagtctat
      841 taagtttatt ttttcccttc ggatattaca gattttaatt tggaaaaata agaattgatt
      901 tctgcgacag tatacatata ctggatttgc gttttagcca gctgcaaatt ttctttttct
      961 aaatccacat tgttgccgtc taaacgcatg gataggttat cattttcctt aatgataata
     1021 ttaccggata aaatttgctc gtttatttca gatgtagttt ttccgtatgt attttttaga
     1081 ttagccgata gaatggtttc aaaatcaact gtcttgcttt tgtatccggg agttgaatag
     1141 ttggctatgt tttccattgt tgtttgttgt ctaagccata aaccatccaa acttttcttt
     1201 aacaaattta aatttacatt gactctcact tcttgcccct ccaacaaatt tatgaaataa
     1261 agagtgattt gaaaatatcc aaaaaagaaa tgtataatta ttgacagtta ccaaaataac
     1321 tatgaattat acatttcttt tgatataaat catacatggc aaccggctac catagacaag
     1381 tctttaggtc caacagcttt gcgtcactac ctttcgatag ttttgccctt tacacataag
     1441 tattaagttg tataaatttg taaagtaatc ccattataaa tgaggttaaa acaaaatgca
     1501 agcctttttt acaaaatttt acaaaaatct ttatacattt tcttgattta ttattcgtat
     1561 aatgccttta tatttataca tcctcatgcg ttattttctg gtgcattaca gcgaataaaa
     1621 aaatgggtat tgcaaaaaca cccataattg ttataaatat tatttttttt cgactagcaa
     1681 aattagcata attataaatt atgcatcaaa atatcaaaaa aatattacat ttttttaatt
     1741 ttttattgct tttaatttgt tttttatatc atttaagctt atttcattta ttttttcaga
     1801 agcaatttta ttaaattcac aagtttcagc caattctttt ctggtaattt ttatgtcttg
     1861 aggagcactg atagcaattt taactttatc ttgacttatt tctgaaataa ttatctcaat
     1921 atcatcaccg ataagtatgg attctgatac ctttcttttt atcactaaca tttattaccc
     1981 tcctcagttg atactatatt gtatctgagt ggatattcat tttgcagtat aacctgctta
     2041 gctgttctgt tattaggatt aattgcaatc gggcttttta aattaaccac ggattcttta
     2101 atatcgtctt ttataattgc aattaccaaa tacttcaatt ctgccgtgcc ggttgatttc
     2161 agctcacata aatcatcttc gtttaaaatc ggattataat ctataaaaat agcatacgga
     2221 tcaatcaaca caaaagatag ttccatatta tcaagtgatt gaagaaacat tatatcatcc
     2281 tcgggactat cctttaatat aatatatttt ttgtaatcat caaatccgta tatgcctgtg
     2341 gtgaaattta ttatatcttc ttcaccaacc aaaatttctc caaaatctct tgttttaatt
     2401 ttcatttttt cagacaaggg tatctatctc cttataatta ggattcgcac tgggcggcac
     2461 aaaaatcggg gtacctacat attttatttc tacgcttggg tattcactga taataaattc
     2521 tatacttccg ggaataaatt ttatttgagg tttttcaaca ttccagtcaa aatctaattt
     2581 atccattgca aaactcatgg aaattcctcc tgtatcccat gaaatatcag gacctgtgga
     2641 agggataaat ccaatattaa aactcacatc actgatgaac tttttcattg caatttcagg
     2701 tattggatta tccataatat tgatatccaa cataaggttg ccttcccttg cataagttgc
     2761 agttgcttca taagcagctt gtacaccttt tttggcaaca tcactaatca agtgcattgc
     2821 actttttaat cctgccgaat atcttgcttc tacagtatcg atttttattt tggcaggctt
     2881 catctgcatt tgaaggccgc ccttgtctct tgatacttca aaagtggcat cggtagttgc
     2941 cagttcaagg cgagctctat taattttcat ttcgaatgcc attggaatag ttttaatttc
     3001 taatagcggt tccatattat tttctccttg ctatttcata aaatctataa aactattttg
     3061 cagaatccta ttgcccattg ataatgctgc attataagaa tattcctgca tcttaaagtt
     3121 tactattgct tctgcagggt cttcaaattc aacttccaat aattttttat ttaaattgaa
     3181 ttgattatcc tcatttcttg tctttaaaaa gtccaaataa tttgttttag ccccaatatc
     3241 ggtaatatga actaatactt gttttttttg ctcttcaaac cggtctatgt atggtgaaat
     3301 tttatctatt gaaaagtccg gatttctcaa ttcgtccttt atttttccta ttaaattata
     3361 taggttttcc ggattatcgt ccgttcctcc ataccccata aactttatgc ccgacattga
     3421 catatcaaat actgtgtcag tgtttaattc tccgtcaccg ttaaaagtca ttcctaagcc
     3481 taagtctatg ttaattgttt cattcgctaa tttatctaag gcttccttat tggcaggatc
     3541 attaacatca agtcccttaa ataataaatt tccattcata tccaatgtaa aagggatttc
     3601 ttccttgttt gttccaccga aaacatatct gtcttcaaac tttgcattga gagatgtcag
     3661 tatggagcgc tgcaaatttt caagctgctt tgcaataatt tctctgtcct catcattcat
     3721 tgttccatta atacctttta aatatgaggt gtaaactgat tccatattgt tgcttatttc
     3781 cgtcaaattt gtttctgcag ctgtaagaaa tgattcaaca tctcgtatgt tggtatcgta
     3841 tatctcggtt gttctatatt ctcttctgag cttgtatgct tttattgcag atacagggtc
     3901 ttcagagcct ttaacaaact tcctgcctgt ttcaaccttc tcacttaaat aattcatttc
     3961 ataagcagat ttgtttaaat tttttatata tttatttgac atcatacttg tagttattct
     4021 catactatca cccctatact cccattctgt ttatcaatgt ttccagcatt tcatccaagg
     4081 tggtcataag ccttgcggct gcattgtatg atttgctgta ctgaagtaaa tttactcctt
     4141 cttcattcaa atccacggaa gacatgctgc ttcttgcata gtctatatga aattgggttt
     4201 ctgaatatgt atcaaatgat atttgtacat cagctgtctg cagatttaat tttgttgttg
     4261 tatatgacaa aaattcttgg aatgtacctt taaacagtat atcatttgta gtgcttgggt
     4321 ctctgtcaac atcaaattca ttcttagcat taaatgcatt tatcattcga agtatattgt
     4381 caacagctcc ggtattatcc tcatctacgc cagctttctc cttaatagtg ttggttatat
     4441 atgagtctgt tgcacttgcc cagtcatctg atatttttat gttgccggct cttatgattt
     4501 ctgtatcagg gtcagatgga tctgcacctc ttgctccgaa tagaggtttt gtaatccatg
     4561 tctgtacatt tccgtctatg tcaaatgaat ccggcttcct gttcagctca ttcataactg
     4621 ttgcgaattt atttgctaat gtgtctaaca tattattata atattgaata cctttaactt
     4681 ctgctgcctt ttcatatcca ttaaccgggt caatatcatc caccgtggca aattcgccct
     4741 ttccgttaag gaactttaag taaccgtcta tttgaccgcc ttttataaaa ttggtaatat
     4801 cgtccgtatc tgttgcacct gttaattcta ttttaattaa tccattttgc tcattaacag
     4861 aaattgtgtt gctattattt ctattaacca attcatatgt tgtcccgctt aatctgataa
     4921 ccaactcgtc tattgtcttg cctccaccta tattgagagg ctcaatatct acttctatat
     4981 ctatatagtt ggaaagctca tctaccagca tatttctttc atcattaagt tccaatgccg
     5041 gattgccgct tatattgctt tctttaattt gctgatttaa tttggctatg ttttttgtca
     5101 actcatttac tttttcaaca gctccatttt ccagataatt aagttgttga ttgtatatag
     5161 tttctatatc tcttgaataa tcattgaaca tttgagtaag aatttctgca gatgttctga
     5221 caacaccttc cattacaggg tctgaaggac tgcttgtcag tgaatgaagc tgtgttaaaa
     5281 ggtcggaaaa ttgcgcatct aatttatcca ttgatatttc atcaaacact gattccaagt
     5341 ctctcaaagc ttctaattga acctgctcgc tgcctgcctt tgaagtttcc attctatatc
     5401 tcaaatcaag aaatgcatct ctaaactggc tcacaccgtt tgcattaact ccttgtccta
     5461 ttacaggtcc cggagtgcca tactttaaat tgcttgtgtt aaaagcaaca gaacttatgt
     5521 caagacgctg cctggtgtat cccttagtat ttatattcga aatattttgc cccactacat
     5581 ccatgtggtt ttgatttact tttaatgcac ttgttgctgt tttatatcct aaaaaagttg
     5641 acctgagcat agcttctcct aactttcttt tatattttat gtgaaatcaa taaaccttcg
     5701 gttacatttt tgcttttgtc atttgtatat atattttctt tttctcctaa attgttcatt
     5761 ttattgttta tcctgcgaag tcttacttca ataacaatct ttaataaatc gtttatttta
     5821 tttaattcct taattttatt atttaattct tcaaattgtt cttgtaattt tattttagat
     5881 tcattttccg ccgcatcaat tatttctttc aaagtcttgc cgcttaattc caatgaattt
     5941 agaagttttt ctctcttatg ttcaattcct ttcatttgca gataatatac ttcttcttgt
     6001 gacactatat tatctaatgc aattacatca tcttctatta ctgcttcata tttatcatat
     6061 tctagttcca aaaacatttt gtaaatttcg gtgtattctt ttaataattg gtataaagaa
     6121 tccataatag ccccctacct taataacctt cttactattt cttcaacatc tatgctataa
     6181 gttttattgt ttatgctgtc cttaattctg ttaattttat cagcatttgt ttcttcgtta
     6241 atttgtgata ctactttctt cttaatagat tgaaggctga tattttcctt atttttatta
     6301 ttaaaatcta ttacatcata tttattttct ttattgattt cttctttttt gattagatta
     6361 gaattaccct tatagtttat ataattatta ttgttgatat tattaatttt cataacgtcc
     6421 ctcttctatt tattttcata taatattaat cgacagaaat acaaaaaaat ttagctaatt
     6481 tcatcatgca ttgctcatta ttttttcttc atctataact aaaagaacct cttcactttt
     6541 atgtcctttg gcaacacccg ttataaagcc gttattacac atttgtttaa tttcttcggt
     6601 ctcttcataa gcaaaaatat tctctacaga taaaacttca tctacaacga ggcctataaa
     6661 cgaattgccc ttttccaaga ctacaaccat agctttgttt tcttttgaat tattggccga
     6721 attgtttccg gattcatctt cggtattcaa taataatttt aagtctacca agggaattac
     6781 gtttcctctc agatgtatca atccgataat atgctccgct acattaggca caaatgtaac
     6841 atcatccggt ttgataacaa tcgatgttat atacttactg tttacggtgt ataaggtttc
     6901 attaagctta aatatcagcc agggaatgac ctctcgcatt tgcataatat tttcattatt
     6961 cattgtaatc tcccattatt tcatctttaa ttaattccaa ggatccaatt tctttttcgt
     7021 taagtaactt attaaggtca attaatataa ctattttttt atctaatttg gcaattcccg
     7081 ttatataagt aataacataa ttattgccca cctgcggcgg ctcggatata ttttcataat
     7141 atatatttat aacttcattg acggaatcaa caataaagcc gatgtataag ccttcgatgt
     7201 ttgtaacaat tatacatgtc ctttcattgt attcaatttc ctgttttttt agccttatcc
     7261 tgacatcaat tatgggaacg ataatccctc ttaaattgat aattcctttt gcatagcttg
     7321 gaaattcagg gacttcttca atctcttgca tccccgttat ttgtacaaca tgagcaatgg
     7381 gaattccgaa tacttgattt tcaatataaa aagtaagata tttattaact tgagcatctt
     7441 cttcttcaat atatgtatgc aagctattaa atttgtccat aacttcacct cgtgatatca
     7501 ttctatataa gttatcgtca tgtttaataa aaacattaga aaaatttaca taataccggt
     7561 taagctatgg ggcataaaaa aacagccgat agatttctct attgactgtt tagtattttt
     7621 ctttttacgg gattacatca ttcccattcc gccgcccatt ggtggcattg caggttcttc
     7681 ttttttaggt tcgtctgtta ctgccgcttc tgttgtcaat aacattgcag ctacgcttgc
     7741 tgcattttga agggctgatc ttactacctt tgttgggtct acgatacctg cttcaatcat
     7801 atttacatat ttttcgttta atgcatcaaa tccgacacct ttttcgctat ttttgatttt
     7861 ttcaactatt actgagcctt caagacctgc attgattgca atttgtctga ttggttcttc
     7921 caatgctttt tcgattatct tagcacctgt ctttgcatct ccgtccaatc cttctgctac
     7981 cttgttaact ttatcgattg ttgttaaaag tgcaacaccg cctcctgata ccaagccttc
     8041 ctcaactgct gctcttgtag catttaaagc atcttcaatt cttaatttct tttctttcat
     8101 ttcggtttct gtagctgctc caacattgat aactgccact ccgcctgtta atttagctaa
     8161 tctttccatc agcttttctc tgtcgaattc agaagttgat tcatcgtgct gagctctaat
     8221 ttgtctagct ctttcttcga tttcttcctt tgaacctgca ccgtttacta ttacggtatt
     8281 ttctttgtct attttaacag ttccggcatt accgagcata tctaaggttg tttcttttaa
     8341 ttctaatcct aactcttcgg atattacctg gcctcctgtc agaattgcga tgtctcttaa
     8401 catttctttt cttctgtcac caaagcccgg agcttttacc gctacacaat taaaggttcc
     8461 tcttaattta tttactacca atgtagctaa tgcttcccct tctacatctt ctgctataat
     8521 aagtaatgat ttgcctgttt gaacgatttg ttccaaaacg ggaagaacat cctgaatgtt
     8581 tgtaattttc ttgtctgtta ttaaaatata cggatcttct aaaacagctt ccattttttc
     8641 tgtattagtt accatatatg ccgataagta ccctctgtca aactgcatac cttcaacaac
     8701 gaccagctct gtatccatag tctttgattc ttcaacagtt ataacgccgt ccttgccaac
     8761 ctgatccatt gcttgtgata ttaattttcc tatttcttca tctcctgcgg aaatagaagc
     8821 aatttgagca atttcttctg atgtttcaac tttttttgtt atgtttttaa gttcttccac
     8881 tgctgctgca actgcttttt cgataccttt tttaagtatc atgggattag cgcctgctgc
     8941 aacatttttg agtccttctc ttattattgc ctgtgccaat aatgttgctg tagtagttcc
     9001 gtcacctgct acgtcatttg tttttgttgc aacttctttt accagttgtg ctcccatgtt
     9061 ttcatacggg tcagaaagtt caatttctct tgctattgta actccgtcat tagtaatctg
     9121 cggtgagccg aatgatttgt ttaaaacaac atttcttccc tttggcccta aggtaacttt
     9181 taccgtatct gctaatgcat taacgcctct ctccattgcg cggcgtgcat cttcatcata
     9241 tttaattatt tttgccatat ttatctctcc tttttattca ataactgcca atacatcagc
     9301 taattttatt ataattaatt cttcaccgtc aattttcact tcagttcctg caaatttaga
     9361 gtaaataacc ttgtctccta ctttaatttc atcttttttc ttttcatcgt ttgtaattcc
     9421 cgcaccaaca gcaagaactt cagcaacctg cggtttttct tttgcagagc cgggcaatac
     9481 aatgccgctt ttggtttttt cttcggcttc aatcatttta attactactc tttcgcctaa
     9541 tggttttact gtcattatca attcctccta tatttaatgt taatatatac gattttttgt
     9601 tagcactcaa ctccaaagag tgctaacacc tattctttat attactcatt tgcctttgtg
     9661 ttttcaaccc ctatttaccc caattaacac ccataaacat attaatatat aattattcct
     9721 accgttttct ttcctttgct ttaaattgct ccatatttca taacaaaaga ccgtgacaag
     9781 gggacggggg tattgtcata tgacaagggg acggggcgtt gtcataaaat atattttaaa
     9841 taatagatgt tttttatgcc gatacgttgt ataatgaatt taccagtata atacatagga
     9901 gggtttaaaa tggctcttgt aacatcacaa gaaatgttta agaaagcata tgagggcaaa
     9961 tatgctgtag gtgccttcaa tgtaaacaac atggaaatca tacaaggaat agtggaagca
    10021 gcaaaacagg aaaatgcacc tcttatactc caagtttcgg cgggcgcaag gaaatatgca
    10081 aatcctattt atttaagaaa gcttgtggaa gcagcaattg aagacacagg attagatatt
    10141 tgtctccact tagaccatgg tgaagatttt gaaatttgta agcaatgtgt tgatgatggt
    10201 ttcacctcag ttatgattga cggctctaaa tatccttttg aagaaaacat agctcttacg
    10261 aaaagagtgg ttgaatatgc tcactccaaa ggggttgtag tagaagctga gctgggtaaa
    10321 ttggcagggg tagaggatgc agttaaagta agaaccaaag atgctacata cacagaccct
    10381 gatgaagctg tggaattcgt ggaaagaact ggagtagact cattggcaat tgctatcgga
    10441 acaagccatg gtgcttataa attcaagggc gacccgactt tggactttga aagattggaa
    10501 actataacaa accttctccc gggtttcccc ttagtactgc atggttcatc ctcggtacca
    10561 aaggagtttg tggatttatg caataaatac ggaggtcaaa tcccgggagc tgcaggggtg
    10621 cctgaagaaa tgctaagacg tgccgctcaa ttaggggtat gtaaaataaa tatagataca
    10681 gatttaagac ttgctcttac cgcatgtatc agacaatttt ttgttgaaca tccttctgaa
    10741 ttcgacccga gaaaatattt aggacccgga agaacagcga ttaaagatat ggttgctca
//
LOCUS       DAOSHP010000064         9842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_5870_length_9842_cov_8.821600, whole genome shotgun sequence.
ACCESSION   DAOSHP010000064 DAOSHP010000000
VERSION     DAOSHP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5870_length_9842_cov_8.821600"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="whiA"
                     /locus_tag="PLB33_06915"
     CDS             complement(<1..937)
                     /gene="whiA"
                     /locus_tag="PLB33_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOG63292.1"
                     /translation="MTFSSRVKDELSRTEISDVLCANAEAAAFVRTVGYITIKGLNKV
                     EFEFLTENAAVARRIFKVLKIAHNINADVSVSKSNRLKKQNNYIIKIDEKLTKQFLSD
                     SQIIKDNDFNVMNFNYEVPEELLASDLCRRAFIKGSFMGSGSISNPEKSYHAEFVSNK
                     EVQSKTLQKLLKIYGINGKNIYRKNNYVTYIKESEQISDLLALMGANNAVLDFENIRA
                     VKETRNQINRVINCETANLDKIVDTSMRQINNIKILKKYKVIDQLPDNLRELAYLRLK
                     HSNASLKELGQMLDPPLGKSGVNHRLRKIEEIAEDL"
     gene            complement(946..1371)
                     /locus_tag="PLB33_06920"
     CDS             complement(946..1371)
                     /locus_tag="PLB33_06920"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOG63293.1"
                     /translation="MVDEISAGGIVFFKNNILMLKKYNGDWVLPKGRVEENESLKETA
                     LREVYEETKAKVTPIKYLGKISYEFSRTCYLDKIHIRKEVHWYLMMARNMNCTAQKNE
                     GFIDAKFLPFERSLTIARYDDERKIIKKAVDDIKFHSYK"
     gene            complement(1503..2645)
                     /locus_tag="PLB33_06925"
     CDS             complement(1503..2645)
                     /locus_tag="PLB33_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOG63294.1"
                     /translation="MEKIKLAAISAFGLEAVVKRELTDLGYENIVTDNGWMYFDAEIK
                     DICKTNINLRCADRVMLVMGQFETLSFEELFDKTYELPWEKWITKDGKFTVNGKSVKS
                     KLYSTPDCQRMVKKAISKKLCEYYDTDWMPETGAEFTILVSIHKDIATISIDTTGARE
                     GLFKRGYREKSMAAPLKETMAAALVKLSYWNKNRVLYDPLCGSGTIAIEAALIGRNIA
                     PGLNRNFASMEWPLVKDEYWKNEKIEARKRIDFDSEIKIYASDVSEKAIRIAKENAIE
                     AGVDDCIEFFVKDVTHIEKPMCSYGVLITNPPYAERIGDEEQLTKIHKKLGQVFGRDK
                     TWSVYVITSSVNFEKEFGRKADRKRKLFNGDMRVDYYQYFGEKMPE"
     gene            complement(2717..4144)
                     /locus_tag="PLB33_06930"
     CDS             complement(2717..4144)
                     /locus_tag="PLB33_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG63295.1"
                     /translation="MNNEIEFSKQDREKFEYILDNIPDAITIVDNYGKVIFFNSTSEE
                     YFNLKKSDIIDKDLVEFFPTAILLRVLDSKTSFYNIYNSPRENSFVVSSAAPLYDRNG
                     NMIGALARDRDITEIVRLSELLNKTQSNLLKLEKEYSKVTSSGEAYFSKIISNNAAFI
                     EIINLCKNISKTPLNILLKGESGTGKELFAKAIHYESGRKGKFIPINCSAIPRELFES
                     EIFGYEAGAFTGAKSEGKKGKFEEAEGGTIFLDEIADLPLELQPKLLRILEDGVVTRI
                     GGNKPKTIDVRIISATNKDIRELINEDKFRKDLYYRLDIFQVNIPPLRERKGDIILLA
                     NRFLQEFCMEQGISIVEIPDEIMSIFSEYRWEGNVRELRNVIQRCAVIASQRGKNKIE
                     REYLPSYMQDIEVDEIKKLINLNEIKSYSGLEEYIQQIEKRIIIKTLEEMNYNKKETA
                     KKLQIPRATLYYKMDKYGISRDDKY"
     gene            complement(4226..5377)
                     /locus_tag="PLB33_06935"
     CDS             complement(4226..5377)
                     /locus_tag="PLB33_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOG63296.1"
                     /translation="MNIDEIIKNSLTENKFIYCIFTTPRVKSQYKKITVRPIAIKDRE
                     YLQLEKFTETKAFHENLDYEVALDVLLNMVNEYRNINLFTTDADYQILVSKKGSIKIT
                     RSEPVKKLKKEEHNKKKQYLISENQPCDFLIILGIMNKDGKVYTKKYDKFKQINKFLE
                     IVDDSLAGKSIKDGFTIIDFGCGKAYLTFALYYYFYYIRRIKVKITGLDLKEEVIGFC
                     NETAKKLNYENLNFVYGDISDFEYKNKVNMIVTLHACDTATDAALVKAIAWDCDIILS
                     VPCCQHELFNKIENENLNPMLKHGLIKERMSSLITDSLRGLFLETKGYKVQLMEFISM
                     EHTPKNILIRAVKSNKNTESAEIEYEKFKKYWNLNNLFIENYYETIINP"
     gene            complement(5393..7075)
                     /locus_tag="PLB33_06940"
     CDS             complement(5393..7075)
                     /locus_tag="PLB33_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOG63297.1"
                     /translation="MSEFNNENENRESVNFIQKIINEDLEKGTYGNRVHTRFPPEPNG
                     FLHVGHAKSICLNYGLAKKNNGIFNLRFDDTNPTKEDDIFIESIIKDVKWLCPDWEER
                     IFYASNYFGKMYDYAVELIKKGLAFVDDLSADEIREYRGTLTEPGKESPYRNRTVEEN
                     LELFEKMKNGVFKDGEKVLRAKIDMASPNINMRDPVIYRILHESHYNTGDEWCIYPMY
                     DFAHPLEDAIEGITHSICTLEFEDHRPFYDWVLSNIDDFKDAPTKQIEFAKLFLSKTM
                     MGKRYLKQMVEEGYVDGWDDPRMPTIAGFRRRGYTPEAIHNFCEAIGVSKAQSVVDIA
                     MLEFAIRDDLGPKVPRTMAVLDPIKLIITNYPEDKVEYVETENNPDNPDMGSRMIPFT
                     REVYIEREDFMENPPKKYFRLFPGNEVRLRNAYFVKCVDFVKDEDGNVTEVHCTYDPE
                     TKSGSGFTGRKVKGTLHWVSAKHCIDAEVRLYDYMMEDEEYDKTNFLEKLNPESKVVL
                     KNCKVEPSMKEFKVGDRFQFLRHGYFIIDQDTTEDNLVFNRIVSLKSSFKGV"
     gene            complement(7314..8168)
                     /locus_tag="PLB33_06945"
     CDS             complement(7314..8168)
                     /locus_tag="PLB33_06945"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOG63298.1"
                     /translation="MEIKKICIVGTGTMAKGIVQTFAQAGYPVIVKGRPGNTFDTLET
                     VIGKGLSKLVAKGKIEQSKMDEILGNITKTTTYEECADADLFIEAVAEDMVTKHAMFA
                     DIEAVAKEDAILSTNTSSLSITEIAAKSKNPGRIIGMHFFNPVPVMKLVEIIKGKLTD
                     DEVHAKVFELAQKIGKTPVSVEEAPGFVVNRVLIPLVNEGIGILADGVASAEEIDTAM
                     KLGANHPMGPLELGDLIGLDVCLAIMEVLYNEFGDPKYRPHPLLRKMVRANLLGRKTG
                     KGFYDYSK"
     gene            8330..9166
                     /locus_tag="PLB33_06950"
     CDS             8330..9166
                     /locus_tag="PLB33_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOG63299.1"
                     /translation="MIKKFLIFATKLINKTKEDDILALSAQLSYYLILSIFPFLILAI
                     SLMCGYSEYIYSILNSLSDVIPEEVYRIIYNVLKYSVASCSKPYLTISMLIIIWSATS
                     GSAAIINGINIAYGFNTKKNFLFLRIRGILFTLALMVSMQIIFAAVVAGRTLLLFVKG
                     ISIFEDYNFIFIDILRFAVTFFIVLIILLASYKFLPYEKVKFSYAYPGAIFAAAGSIA
                     GSYLYSRYVSTRVVFINSIYGNLSGLFVFIIWIYILSIIFLMGAEVNYFAAKQKNGIN
                     GK"
     gene            complement(9227..9832)
                     /locus_tag="PLB33_06955"
     CDS             complement(9227..9832)
                     /locus_tag="PLB33_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016429 - tRNA
                     (adenine-N1-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HOG63300.1"
                     /translation="MEQYFTENPTTEKEIYKFEWNVGKEVLYFYTSNSVFSKKGVDFG
                     SMLLVETILNENENFSGSILDLGCGYGPLGIIVAKKLRKSFVTMCDVNERALELSRMN
                     AKENKVDDRIKVIASSAFENIKDNYDIIMTNPPIRAGKNVVFSFYEGAYEHLNNGGKF
                     YVVIQKKQGAPSTKAKLESLFGNCETADKKSGYFIFRCIKK"
BASE COUNT         2713 a         1965 c         1415 g         3749 t
ORIGIN      
        1 ttaaatcttc agcaatttct tctatttttc ttagtctgtg atttactcct gattttccaa
       61 ggggagggtc taacatttga cccagttcct ttaaacttgc attgctgtgt ttaagtctta
      121 agtatgcaag ttcccttaaa ttatccggaa gttggtctat aaccttgtat ttttttagta
      181 ttttaatatt gtttatctgc ctcattgatg tgtctacaat tttatccaga tttgcagttt
      241 cacaattaat tactctgttt atttgatttc ttgtttcctt aacggctctt atgttttcaa
      301 aatccaagac agcgttattt gcgcccatga gagctaacaa atcggaaatt tgctcgcttt
      361 ccttaatata tgtcacatag ttgttctttc tgtaaatatt cttaccgttt attccatata
      421 ttttcaaaag cttttgcaaa gtcttgctct gtacttcctt gttgcttaca aattcagcat
      481 gatatgattt ttcaggattg cttatcgaac cgctgcccat aaatgaacct tttataaagg
      541 ctctcctgca taagtcgctt gccagtaatt cttcaggtac ttcataatta aaattcatta
      601 cattaaaatc attatcttta ataatttgac tgtctgaaag aaattgcttg gttaattttt
      661 catctatttt aattatataa ttattttgct ttttcaatcg attgctttta gaaacactga
      721 catctgcatt tatattatgg gctattttta aaaccttgaa tatccgtctt gcaactgctg
      781 cattttcagt caaaaattca aattctacct tatttagacc cttaattgtg atatatccca
      841 cagttctgac aaatgctgcc gcttcagcat tagcacataa tacatcactt atttctgttc
      901 tggacagttc atctttaact ctcgatgaaa atgtcatggc atcacctact tatatgaatg
      961 aaatttaata tcatctacag cttttttaat gattttcctt tcatcgtcat atcgagctat
     1021 tgtcaatgat ctttcaaagg gcaaaaattt tgcatctata aatccttcat ttttttgagc
     1081 agtgcagttc atatttcttg ccatcattaa ataccaatgg acttcttttc ttatgtggat
     1141 tttatcaagg tagcaggtgc ggctgaattc gtaacttatt ttacctaaat acttaatagg
     1201 agttactttt gctttcgttt cttcatatac ttcccttaag gcagtctctt tcaaggactc
     1261 gttttcttca actctgcctt ttggaagaac ccaatcgccg ttatactttt ttaacatcaa
     1321 tatgttgttt ttaaagaaaa ctattcctcc tgcacttatt tcatctacca tacttgcctc
     1381 accttttaat attgtagtct ttataatacc acaatattaa gaaaaatcaa acatatattt
     1441 acatatttta agtttacaat aaaaataaat atatttcaat cttttcattt cggtgcgcag
     1501 tctcattcag gcattttttc tccgaaatac tgatagtaat caaccctcat atcgccattg
     1561 aaaagttttc ttttcctgtc tgcttttctc ccaaattctt tttcaaagtt tactgaagaa
     1621 gtgattacat atactgacca agttttgtcc ctgccaaaaa cttgccctaa ttttttatga
     1681 attttagtaa gctgttcttc atcacctatt ctttccgcat atggcggatt ggttatcaat
     1741 actccatagg aacacatcgg tttttcaata tgtgtaacat cctttacaaa aaactcgata
     1801 cagtcatcca ctcccgcttc aattgcattt tcttttgcta ttcttattgc cttttcactc
     1861 acatctgaag catatatttt aatttctgaa tcaaaatcaa ttctttttct tgcttcaatc
     1921 ttttcatttt tccaatattc atctttaacc aatggccatt ccatggaagc aaagtttcga
     1981 tttaaaccgg gagctatatt tcttccgatt aatgcagctt caattgcaat tgtacctgaa
     2041 ccgcataatg ggtcatagag cactctgttt ttattccagt aactgagctt taccaaagct
     2101 gcagccatag tttcctttaa aggtgccgcc attgattttt ctctgtatcc tcttttaaac
     2161 aatccttccc ttgccccggt tgtatctatt gaaatcgttg caatgtcttt gtgaattgaa
     2221 actaatatgg taaattcagc ccctgtttcc ggcatccagt ccgtatcata atattcacag
     2281 agttttttgg aaattgcctt tttaaccatc ctctggcagt caggggtgct gtagagtttt
     2341 gatttgacgg attttccgtt aacagtgaac tttccgtctt ttgtaatcca tttttcccaa
     2401 ggaagctcat aagttttgtc aaataattct tcaaaactta atgtttcaaa ttgtcccata
     2461 acgagcatta ctctgtcggc acacctcaaa ttgatatttg ttttgcatat atctttaatt
     2521 tctgcatcaa aatacatcca cccgttatca gtaactatat tttcatatcc cagatctgtc
     2581 aattcccttt ttacaactgc ttcaagacca aaggctgata ttgcagccaa ttttattttc
     2641 tccatacatt tcctcccaac ggggcatccc ttcatggcta caccccgtat attaagggga
     2701 cgggggtacc gtcatcctaa tatttatcat ccctgcttat tccgtattta tccatcttgt
     2761 aatacaaggt ggctcttggt atttgtaatt tctttgctgt ttctttctta ttatagttca
     2821 tttcttccaa ggttttaatg ataattcttt tttctatttg ttgaatgtat tcttccaatc
     2881 ccgaatagct ttttatttca tttaaattaa taagcttctt aatttcatca acttcgatat
     2941 cctgcatgta tgaaggaaga tattctcttt caattttatt ctttcctctc tgagatgcta
     3001 ttaccgcaca tctttgtatt acatttctca gttctcttac attaccttcc catctgtatt
     3061 cggaaaatat actcattatt tcgtcgggga tttccactat gctaataccc tgctccatgc
     3121 agaattcctg tagaaacctg tttgctaaaa gaattatatc tccttttctc tccctgagag
     3181 gaggtatgtt tacctgaaat atatcaagtc tgtaatagag gtccttcctg aatttatcct
     3241 catttatcag ttctcttata tccttattcg tggcagaaat tattctgacg tctattgtct
     3301 ttggcttatt tcctccaatt cgagttacta cgccatcttc caaaatcctt agaagcttgg
     3361 gctgaagttc taagggcaag tctgctattt catccaaaaa tatggttcct ccttcagctt
     3421 cctcgaattt tccttttttc ccttcagatt ttgctccagt aaaggcgcct gcttcatatc
     3481 cgaaaatttc actctcaaac aattctctcg gtatggcact gcagtttatc ggaataaatt
     3541 ttcctttcct tccgctttca taatgaattg cttttgcaaa gagctcctta cccgtgccgc
     3601 tttctccttt taacaatatg tttagaggtg ttttggatat gtttttgcac aaatttatta
     3661 tttcaataaa agctgcatta ttgctaatta ttttagagaa atatgcttct cccgaggaag
     3721 ttacttttga atattccttt tcaagcttca ataaatttga ctgggtttta ttcagtagtt
     3781 cgcttaatct tacgatttca gtaatgtccc tgtcccttgc caatgcacca atcatgtttc
     3841 cattcctgtc ataaagggga gcagcactgg acacaacgaa gctgttttcc ctggggctgt
     3901 tgtatatatt ataaaaagat gtcttgctgt ccaatactct taacaatatt gcagtgggaa
     3961 aaaattcaac aagatccttg tctattatgt cgcttttttt caaattaaaa tattcctctg
     4021 atgttgagtt gaaaaatatc actttgccgt aattatcaac tattgttatg gcatcaggaa
     4081 tgttgtccag tatgtattca aatttttctc tgtcttgttt gctgaattct atttcattat
     4141 tcatgtaatc ccccctggaa acaataatat tttgggcaag ctctcatctg ttgcatatac
     4201 caaagtggta tgcaataagt taaaattacg gatttatgat tgtctcgtag taattttcta
     4261 taaaaaggtt attgagattc caatatttct tgaatttttc gtattcaatt tccgctgatt
     4321 ctgtattttt atttgactta actgctctta tgagaatgtt tttaggagta tgctccattg
     4381 atatgaattc cattaactgc accttatacc ccttagtttc caaaaacaaa cccctcaatg
     4441 aatcggtaat taaggaggac attctctcct ttatcagccc gtgcttaagc atgggattta
     4501 aattttcatt ttcaatttta ttgaagagtt catgctggca acaaggtacg gacaaaataa
     4561 tatcgcagtc ccaggcaatt gcctttacaa gggctgcgtc tgttgccgta tcgcatgcat
     4621 gaagagttac tatcatattt actttgtttt tatattcaaa atcacttata tctccatata
     4681 caaaattaag attttcatag ttaagttttt tggctgtttc attgcaaaat ccaatcactt
     4741 cttcttttaa atcgagccct gttattttca cttttattct tctaatataa taaaaataat
     4801 aatacagggc aaatgtaaga tatgcttttc cacagccaaa atcaataatc gtaaagccat
     4861 ccttaatact ttttcccgcc agtgaatcat caacaatttc caagaattta tttatttgtt
     4921 tgaatttatc atacttcttg gtataaacct tgccatcttt attcataatt cccaggatta
     4981 taaggaaatc acatggttgg ttttcactta tcaaatattg ttttttcttg ttatgctctt
     5041 ctttcttcag cttttttaca ggctcacttc gagtaatctt tatacttcct tttttgctta
     5101 ccaatatttg ataatctgca tctgttgtaa ataaatttat attgcgatat tcattaacca
     5161 tgttaagcaa gacatcaaga gcaacctcat agtctaagtt ttcgtggaaa gcttttgtct
     5221 cggtaaactt ctccaactgc aggtactccc tgtcttttat tgcaataggc cttacagtta
     5281 tttttttata ttgggactta acccttgggg ttgtaaaaat acaatatatg aatttgtttt
     5341 ctgttagact gttttttatt atctcatcaa tattcaaaat accacctgta atttacacac
     5401 ctttaaagct gctttttaat gatactattc tgttaaatac caaattatct tctgttgtat
     5461 cctggtcaat aataaaatac ccgtgtctta aaaattggaa tctgtcacct actttgaatt
     5521 ccttcataga aggctcaacc ttgcagttct ttaacacaac ctttgattcc gggtttaact
     5581 tttccaagaa attggttttg tcgtattcct catcttccat catgtagtca taaagtctta
     5641 cttccgcatc gatgcaatgt tttgctgaaa cccagtgtag agtccccttt acttttctgc
     5701 cggtaaagcc tgatccgctc tttgtttctg gatcatatgt gcaatgaact tcagtaacat
     5761 ttccgtcttc atcctttaca aaatcaacac attttacaaa atatgcattt ctaagcctca
     5821 cttcattgcc ggggaagagc ctgaaatatt tcttcggagg attttccatg aaatcttctc
     5881 tttcaatata tacttccctt gtaaaaggta tcattctact tcccatgtca gggttatcgg
     5941 ggttgttttc cgtttcaaca tattcaacct tatcttcggg ataatttgtt attataagct
     6001 ttattgggtc taagacagcc attgttctcg gaactttagg tcccaaatca tctcttatgg
     6061 caaattcaag catagcaata tcaacaacgc tctgagcttt cgagactccg attgcttcac
     6121 agaaattatg tattgcttcg ggagtataac ctcttcttct gaatcctgca atagtaggca
     6181 tacgagggtc atcccagccg tccacatacc cttcttcaac catctgtttc aaatatcttt
     6241 tgcccatcat tgttttcgat aaaaatagtt tggcaaattc aatttgtttt gtgggagcat
     6301 ccttgaaatc gtctatattg cttaacaccc agtcataaaa cggtctgtgg tcttcaaatt
     6361 ccaaagtaca tattgagtgt gtaattcctt ctatggcatc ctccaaagga tgagcaaagt
     6421 catacatggg gtaaatacac cactcatcgc cggtgttgta gtgagattca tgaagaatgc
     6481 ggtaaataac agggtccctc atattaatat tgggacttgc catgtctatt tttgctctta
     6541 gaactttttc accgtctttg aatacaccgt ttttcatttt ttcaaaaagc tctaagtttt
     6601 cttcaaccgt tctgtttctg taagggcttt ccttgcccgg ctctgtcaag gtacctctgt
     6661 attcacggat ttcatctgcc gataaatcat caacaaaggc taatcccttc tttatcagtt
     6721 caactgcata atcgtacatc ttaccaaagt aatttgaagc ataaaaaatt ctctcttccc
     6781 aatcagggca aagccattta acatccttga taattgattc tatgaatata tcatcttcct
     6841 ttgtcggatt tgtatcatca aatcttaaat tgaaaattcc attattcttt tttgccaatc
     6901 cgtaattcaa gcatatggat ttggcatgac ccacatgcag aaatccatta ggctccggag
     6961 gaaatcttgt atgtacccta ttcccgtaag tgcctttttc aaggtcctca ttaattatct
     7021 tttggatgaa attaaccgat tccctgtttt cattttcgtt attaaattcg ctcatttttt
     7081 cctcccgcaa tatatataaa caaaataata ttattaaacc taattataat taacaaatag
     7141 ctataattca agtttttctt caatatttat tatatcctgt cttatataca aataatcatc
     7201 cggttatttc tttagtttga ctcaatatca ttaaaatacc cggtgacaac aataaacaaa
     7261 aagaaacggt cataaccgtt cctctttctg ccatcataag cgtaaaagga tctctatttg
     7321 ctgtaatcgt agaatccttt gcctgttttt cttccaagca gatttgctct taccattttg
     7381 cgaagtaatg gatgcggtct gtacttaggg tctccgaatt cattatagag aacctccata
     7441 atggcaagac aaacatcaag tcctattaaa tctccgagtt ctagcggtcc cataggatgg
     7501 tttgcaccca atttcattgc ggtgtctatt tcttctgcac tcgcaactcc atctgccaat
     7561 atacctatac cttcattaac caatggaatc aatactctat ttacaacgaa tccgggtgct
     7621 tcttcaacac ttaccggagt ttttcctatt ttttgagcta attcaaatac cttagcatga
     7681 acttcatcat cagtaagttt tcctttgatt atttcaacta atttcataac aggaacagga
     7741 ttgaaaaaat gcattcctat tattctgccc ggattttttg attttgctgc aatttcagtg
     7801 atggacaatg atgatgtatt agttgatagt attgcatctt ctttagctac agcttctatg
     7861 tcggcaaaca ttgcatgttt cgttaccatg tcttctgcaa ctgcctcaat gaataaatca
     7921 gcatccgcgc attcttcata tgtagtggtt ttcgtaatat ttcccaaaat ttcgtccatc
     7981 ttagactgtt ctattttacc ctttgctacc agcttgctta atcccttgcc tattacggtt
     8041 tctaatgtgt caaatgtatt gcctggtctt cctttaacta taacaggata tcctgcctgt
     8101 gcaaatgttt gtacaatacc cttggccatt gtaccggttc cgactatgca tatcttttta
     8161 atttccattt tatcctccaa ttattttttt ttgcctgttt taattataac attacgatat
     8221 aaaataaatc aagcatttta agaatacttg atgacggtcc ggttccctta ttattatggt
     8281 agattcataa taactgtatt atttgttaat aataatgtag ggtgaatcga tgattaaaaa
     8341 atttttaatt tttgcaacaa agcttataaa taaaacaaag gaagacgata ttctcgcact
     8401 gagtgctcaa ttatcatatt atttaatatt gagtattttc ccctttctta ttttggcaat
     8461 atctttgatg tgcggctata gtgaatatat ctacagtatc ttaaattcct tatccgatgt
     8521 gattccggaa gaagtgtaca gaattattta caatgtgtta aaatactctg ttgcaagctg
     8581 cagcaagcct tacttaacca tcagcatgtt gataataata tggtcggcta cttcgggaag
     8641 tgcggctatc ataaatggaa ttaatattgc atacggattc aataccaaaa agaatttctt
     8701 attcttaagg ataagaggaa ttttgtttac tcttgcactg atggtttcaa tgcaaattat
     8761 ttttgcggca gttgttgcag gaagaacttt attgctcttc gttaaaggaa tttctatttt
     8821 tgaggactat aattttatat ttattgatat cttaagattt gctgttacat tttttattgt
     8881 attaataatt ctattagctt catataaatt tttgccctat gaaaaggtta aattttcata
     8941 cgcatatccg ggagcaattt ttgcggcagc aggctcaata gccggttctt acctctattc
     9001 ccgctatgta agcaccagag tcgtttttat taattccatt tacggaaatt tgtccggatt
     9061 atttgtattt attatatgga tttatatttt atcgattata tttctgatgg gagctgaagt
     9121 aaactatttt gcagcaaaac aaaagaatgg tattaacgga aaatgaccag aggggacggt
     9181 tcttggtgga cagttttcaa ggcgggtgga taattaattc tgttggttat ttttttatac
     9241 acctaaaaat aaaataccct gattttttat ctgctgtttc acagttgccg aataaacttt
     9301 caagttttgc cttggtgctt ggtgctcctt gtttcttttg aattacaaca taaaacttgc
     9361 cgccattgtt taaatgttca tatgcacctt cataaaatga aaatacaaca tttttgcctg
     9421 ccctgatagg agggttggtc ataataatat cataattatc cttaatattt tcaaatgcag
     9481 aagaagcaat aacctttatt ctgtcatcaa ccttgttttc tttagcattc atccttgaca
     9541 attcaagtgc tctttcattc acatcgcaca tagtaacaaa ggattttctt aattttttgg
     9601 caacaataat acccaagggg ccgtatccgc aacctaagtc taaaatactg ccgctaaaat
     9661 tttcattttc atttaaaatt gtttctacta aaagcatgga gccaaaatcc actccttttt
     9721 tagaaaatac actgttgctc gtataaaaat ataaaacttc ttttcccaca ttccattcaa
     9781 atttgtatat ttctttctct gttgtcgggt tttcagtaaa atattgttcc atttttctct
     9841 cc
//
LOCUS       DAOSHP010000065         9629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_6029_length_9629_cov_8.882912, whole genome shotgun sequence.
ACCESSION   DAOSHP010000065 DAOSHP010000000
VERSION     DAOSHP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6029_length_9629_cov_8.882912"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..822
                     /gene="mraZ"
                     /locus_tag="PLB33_06960"
     CDS             385..822
                     /gene="mraZ"
                     /locus_tag="PLB33_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOG63301.1"
                     /translation="MFTGEYIHSFDEKGRVIIPSKFRNELGETFYIGKGLDKCLFVYP
                     IETWTEFVGKLKNLSTFNKEERFFLRRFVSGFSECSFDKQGRILIPPQLRSFSELNTD
                     AAIIGVIDRIEIWNKDTWDEYSNNEQFDFDSIAEKMSELGIGL"
     gene            832..1761
                     /gene="rsmH"
                     /locus_tag="PLB33_06965"
     CDS             832..1761
                     /gene="rsmH"
                     /locus_tag="PLB33_06965"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOG63302.1"
                     /translation="MDYIHKPVLLDESVKGLNIKEDGIYVDCTLGGGGHTEEILKKAT
                     KGKVIGIDQDDYAIERASEKLSGYKNFMPVKNNFSKIEEILNELSIDKIDGIIFDLGV
                     SSFQLDIPERGFSYNYDMPLDMRMDKSQTTTARHIVNGYEENELTRILWDYGEEKWAA
                     RIAKFIVEEREQEFIETTGQLVSIIKKAIPKSARQEGSHPAKKTFQAIRIEVNRELEI
                     LENTVKAAVERLTKKGRICVITFHSLEDRIIKNTFAELNKDCICPPEFPKCMCEHRRK
                     LKIITKKPITPSEEELTENRRAHSAKLRIGERV"
     gene            1765..2229
                     /locus_tag="PLB33_06970"
     CDS             1765..2229
                     /locus_tag="PLB33_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOG63303.1"
                     /translation="MSALRKEAVELHEHELEKEYSPKKRIKRNKRNNTFIESLKNSVM
                     IAITFILGILIVYNYAVITDKQMELNNLNIEIENLNNEIDKYNIALESIKNTNSIEEM
                     AKVYLGMNYPTRKQTVFVDFTYGPDAIEEKNIMAKDENLLVGLIDKVISFIR"
     gene            2243..4360
                     /locus_tag="PLB33_06975"
     CDS             2243..4360
                     /locus_tag="PLB33_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOG63304.1"
                     /translation="MKKRAKSTTNLQNKRRMLFFLVCFFLVTLALVVRLGYIQLIKGE
                     EYRKQAMENWSRDVTISAKRGTIYDAKGKKLAVSINSNTVTCFPADVKKSVNPTVDNS
                     IDEDDSILTAILKKLNRAMFTEETQAEQIPVNTKTPEEIAAILAEILEMDYDEVLEKI
                     TSNYNYVVVKKWITDEQAQKIREQELSGISIIEDNKRVYPYGNFAPYVLGFTNIDQDG
                     LYGVEATYNSYLTGIPGRTVVNTDAYGRELPFGYNEYYESQDGLGVVLTIDEVIQHYA
                     ESAAEKVMADYNAKRATIIVMDPNTSDILAMASKPDYDPNDPRTPIDENIKAEWANLS
                     NEELQNAWFDMWRNPAVNDVYEPGSTFKLITTATALEENVATLGSTYFCDGYVRQITS
                     HNIKCWRYYRPHGQQTLSEAIQHSCNDALAQIGLDIGKDVFYKYVKSFGFEEQSGIML
                     NGEATGIVKKPEYMKDVDVVTQAFGHGVAITPIQLINAVSAISNGGNLMVPRIVKQLI
                     DEDGNIIKNFDTEVRRKVISEETSKIMLQIMKDSAEAGTKQAYRPGYRIGGKSGTAQK
                     VIDGKYIEGKFISSFIGVAPVDNPRAVALVMVDEPEMSIGYYGSIVAGPVAADLLENI
                     LKYYDVEPVYTEEELGHVEKQMVTVPNLLGLTVAEATDKLISAGLESNITIEVDAERT
                     VKSQFPKAGEQVVKSSMVTLTLN"
     gene            4501..5985
                     /locus_tag="PLB33_06980"
     CDS             4501..5985
                     /locus_tag="PLB33_06980"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOG63305.1"
                     /translation="MNIKNLLKEMDYLSYIGEDDIEIRGITNDSRKVNRNDIFVAIKG
                     FTNDGHKFIENALENGASAVICENIPENVKGKSNFIIVKSARASMAKAANIIYGRPSE
                     KMKIAGVTGTNGKTSTTYLLNGIYDYLGVKSGIIGTMGVLIEGKKIKVDNTTPEAFDI
                     QHYLAMMLEKDITHCCMEVSSHALELNRIDEVKIDVGIFTNVTRDHLDFHKTMENYYQ
                     AKKKLFYLTKVNNIINVDDPYGLRLYNELIKEGIKAISVGIENDADIKASNLKTTMNG
                     TTFNLNVMGKEKTAHVNTPGTFSVLNSLGALGAAYALGGNIDDIIEALKQIKGVTGRF
                     EILPNKLDCTIILDFAHTPDGLQKVMETINEFAEGRKIVMFGAGGERDLSRRAPMGEI
                     AGKYCDLSILTSDNPRFEDPYEICKEIAEGVKKSNGEYKIIVERDQAIYYAIDKSQKK
                     DVILLAGKSTEPYQDMGTEKVPYDETMLAKRAIKEVEKKRGLSS"
     gene            6131..7105
                     /gene="mraY"
                     /locus_tag="PLB33_06985"
     CDS             6131..7105
                     /gene="mraY"
                     /locus_tag="PLB33_06985"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOG63306.1"
                     /translation="MQGNGQIIRVIIVSFLIALFLGPVVIPVLKRLKVGQSIREDGPQ
                     SHLAKSGTPTMGGIIIILAILTSIITGRLITKEVLAVIFFMLGFGLIGAIDDYKKVVM
                     RQNEGLKPYQKIVGQLIFSVLLAWYGSRYSAMKTALMIPFVNMHIDLGVLYIPFITFI
                     AVGTVNAVNLTDGLDGLNTGVTLIVMAAFSLIANSLKETNQIYEGISLICAATSGACL
                     GFLKHNANPAKVFMGDIGSLALGGVVSSVAVLSGMVLFIPIIGGIYFVEALSVIIQVF
                     HYKRTRRRVFKMAPLHHHFEMCGWKETKVVSAFWIATLILAFIGIYSI"
     gene            7147..8511
                     /gene="murD"
                     /locus_tag="PLB33_06990"
     CDS             7147..8511
                     /gene="murD"
                     /locus_tag="PLB33_06990"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOG63307.1"
                     /translation="MNVLVVGLGVSGIACIKGLSKTGANIYAFDDSNEEKLRDRLKEV
                     EGIEVEYFFGNEEINKIKFGNLDYAIKSPGIKYETPIIEKLVKNNIEVISDIEAAYRV
                     TNSPIVSITGTNGKTTTTTLIGEMVRESKKTYKVTGNIGFGIYYDALTAKEEEILVAE
                     VSSFQLAGTSKYKPHISVLTNITPDHLDYHHTVENYIEAKFKNVINQDENDYAILNYE
                     DETIRNHSDKIKAKKIFFSSERILEDGIYSENGQMFYRTGNNKLSIINTKDIFIPGKH
                     NLENAMAAGGAAIALGIDIEVISRVLREFKGVEHRLEFTGEYKKVKFYNDSKGTNPDA
                     SIKAIQGIEKPIILIAGGYDKKSSYDDFIKAFDNKVKALILLGQTAEDIQKCALKYGF
                     ENIYRVETMDEAVKKSFELAEEGDNVVLSPACASWGMYPNYEVRGRDFKERVEYYGRN
                     RKKS"
     gene            8486..9607
                     /gene="ftsW"
                     /locus_tag="PLB33_06995"
     CDS             8486..9607
                     /gene="ftsW"
                     /locus_tag="PLB33_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOG63308.1"
                     /translation="MEETVRKAKARSIDWVLVFIIVLLVLFGFLMVYSSSYPDGYYKY
                     GGDPFYFLRKQVIAAGAGLAGMIFFANLPYIIYKKFSKLILFTCIGLAMLTIIIGIAS
                     NGAQRWIEIGGISLQPSEIIKIGAVLYMADYFNRKRSELPSFTKGFLPSLFYIVLFCG
                     LIAIQDDLSTAVILAGTLMVMFFCAGARISHLILLFLLALAGIIALIAMQPYRISRII
                     VFFDPFKYPLDEGYQIINSLYALGTGGLFGMGVGKSRQKFFHLPEAYNDFIFSIIGEE
                     LGFVGAVFVMLGFVVFAWRGLRISMNTEDFFGSQTALGLTVIVLVQFLIHVAVATSSM
                     PPTGRALPFISAGGSSIMFLLCSMGILLNISKYSNTYRS"
BASE COUNT         3459 a         1462 c         2031 g         2677 t
ORIGIN      
        1 tattatgata taatataaag atgagattct tcgagctaaa gctctcagaa tgacatttct
       61 atactcttcg ctaatatgaa gagttttttt tatgcaatga ataagcttat gttatcttat
      121 gcaacagaga agagactcaa agattgtcat cctgagcgaa gcgaaggatc tcatgagatt
      181 cttcgagcta aagctctcag aatgacacgg atgagattct tcagctaaag ctctcagaat
      241 tacaggcaag tacttttcgc tgcgttaaaa tgacaagtat ttcactcaaa aaaatgacaa
      301 aaaaaaattt tttgtatttt ttaaaaataa ttgtggcatt ttatacccac tatgtggtat
      361 aatacaccca gtaggtggta gaaaatgttt actggtgaat atatacattc atttgatgaa
      421 aaaggaaggg ttataattcc ttcaaaattt agaaatgaac taggagaaac attttatatt
      481 ggaaaaggtt tggataaatg cttattcgtt tatcctattg aaacatggac agaattcgtt
      541 ggtaagctta aaaacttatc taccttcaac aaggaagaaa gatttttctt aagacgattt
      601 gtttcaggtt tttctgaatg ttctttcgat aaacagggaa gaatattaat accgcctcaa
      661 cttagaagtt tcagcgaatt aaatacagat gctgcaatta taggggttat agacagaatt
      721 gaaatttgga ataaagatac ttgggatgaa tattccaata atgagcaatt cgattttgac
      781 agcattgccg aaaaaatgtc ggaattgggg ataggcttat aaggaggaag catggattac
      841 atacataaac cggttttact tgacgaatcc gtaaagggac tgaatataaa agaagacggt
      901 atttatgttg attgcactct tgggggggga ggccatacag aagaaatctt aaaaaaggca
      961 actaagggga aagtcatagg aattgaccag gatgattatg ctattgaaag agcgtcagag
     1021 aaattaagcg gctacaaaaa ttttatgccg gtgaaaaata atttcagcaa aatcgaagaa
     1081 attttaaatg aacttagtat tgacaaaatt gacgggataa tatttgacct tggtgtttcg
     1141 tcatttcaac tggatatacc ggaaaggggt ttttcttata attatgatat gccccttgac
     1201 atgaggatgg acaaaagtca gacaaccact gcgagacata ttgtaaacgg ttatgaagaa
     1261 aatgaattga caagaattct ttgggattac ggtgaagaaa aatgggcagc aagaattgca
     1321 aaattcattg tagaggagag agagcaggaa tttattgaaa caacggggca gcttgtatca
     1381 attattaaaa aagcaatacc taaatcagcc aggcaagaag gctcccatcc tgctaagaaa
     1441 acatttcaag caataagaat tgaagttaac cgagagttgg aaatacttga aaatacagtt
     1501 aaggctgcag ttgaaagatt gaccaaaaaa gggagaatct gcgtaataac atttcattca
     1561 cttgaggata gaattataaa aaatacattt gcggaattaa acaaggattg tatttgtcct
     1621 cccgagtttc ccaaatgcat gtgcgaacac agaagaaaac taaaaataat taccaaaaaa
     1681 ccaataactc cgtcagaaga agaattaaca gaaaacagaa gagcacacag cgcgaaatta
     1741 agaataggtg agagggtgta aagaatgtca gcactgagaa aagaagctgt tgagttacat
     1801 gagcatgaat tagaaaaaga atattctcca aaaaagcgaa taaaaagaaa taagagaaat
     1861 aatactttta tagagagttt aaaaaattct gtcatgatag caattacttt tatattagga
     1921 attttaatag tctataatta tgccgtaatc actgacaagc aaatggagct gaataatctc
     1981 aatattgaaa tagaaaatct taacaacgaa attgacaaat ataatatagc tttggagagt
     2041 attaaaaaca ctaacagcat tgaagaaatg gcaaaagttt atttaggtat gaactatccc
     2101 acaaggaagc agacagtatt tgtagacttt acatatggcc ctgatgcaat tgaagaaaag
     2161 aatataatgg caaaggatga aaatctgtta gtaggattaa ttgacaaggt aataagtttc
     2221 attcgataag ggggattgcc atatgaagaa gcgtgcaaaa agcactacta accttcaaaa
     2281 taaaagaagg atgttgtttt ttttagtatg ctttttttta gttacactag ctttggttgt
     2341 aagactcggt tacatacagc tgataaaagg tgaagaatat agaaaacaag caatggaaaa
     2401 ctggtcaagg gatgttacca taagcgccaa aaggggaact atatacgatg ctaaaggtaa
     2461 aaaattagca gtcagcataa attccaacac tgtcacatgt tttcccgcgg acgttaagaa
     2521 aagcgtaaat cctaccgttg ataattcaat agatgaagat gacagtattc ttactgcaat
     2581 tttaaaaaag cttaacagag ctatgtttac tgaggaaact caagctgaac aaatacctgt
     2641 aaataccaaa acacctgaag aaatagctgc aattctggca gaaattttag agatggatta
     2701 cgatgaagtt cttgaaaaaa taacatctaa ttataactat gtagtagtaa aaaaatggat
     2761 tacagatgag caggcacaaa aaataaggga gcaggaatta agcggcataa gtataatcga
     2821 agacaataaa agggtttatc cctacggaaa ctttgcaccc tatgtgttag gctttacgaa
     2881 catagaccag gacggcttat atggagttga agccacatac aatagttatc tgacgggaat
     2941 accgggaaga acagtcgtaa ataccgatgc ttacggaaga gagcttcctt ttggctataa
     3001 tgaatattat gaatctcagg acggattagg tgttgtgctt accatagacg aggtaataca
     3061 gcattatgct gaatctgcag ccgagaaggt tatggcagat tacaatgcaa aacgagcaac
     3121 aatcatagtt atggacccta atactagcga tattttggca atggcatcaa agcctgatta
     3181 tgaccccaat gaccccagaa caccaataga tgaaaacatt aaagccgaat gggcaaattt
     3241 atctaatgaa gaacttcaaa atgcctggtt tgacatgtgg cgaaatcctg ctgttaatga
     3301 tgtatacgag cccggttcaa catttaagct gataaccacg gcaacagctc ttgaagagaa
     3361 tgttgctaca cttggctcaa cctatttctg cgacggttat gtaagacaaa tcacaagcca
     3421 taatattaaa tgttggagat attacagacc tcacggacag caaactttat cggaggcaat
     3481 acaacattca tgcaacgatg cacttgcaca aataggtctt gatataggaa aggatgtatt
     3541 ttacaaatat gtaaaatcat tcgggtttga agagcaatcg ggtattatgc tcaatggaga
     3601 agcaacaggt attgtaaaga agccggaata tatgaaggat gtggatgttg tgacacaggc
     3661 tttcggccac ggtgttgcaa taactcccat acaattgatt aatgccgttt cggcaatttc
     3721 aaacggcgga aatttaatgg tgccgagaat tgtaaagcag cttattgacg aagacggaaa
     3781 tattataaaa aatttcgata cagaagtaag aagaaaagta atatctgaag aaacatcaaa
     3841 aattatgctt caaattatga aagattccgc agaagcagga actaagcaag cttacagacc
     3901 gggatacaga ataggcggta aatcgggaac agctcaaaaa gtaatagacg gtaaatatat
     3961 tgaaggcaag tttatatctt cgtttatagg tgtggctccc gtagataatc ccagagccgt
     4021 ggcgcttgtt atggtcgatg agcccgaaat gagcatcggg tattacggaa gtatagtggc
     4081 aggaccggtc gctgcagatt tactggagaa tatattaaaa tattatgatg tagagccggt
     4141 ttataccgaa gaagagcttg gccatgtgga aaaacaaatg gtgacggttc cgaatctttt
     4201 gggacttacg gttgcggaag caacagataa attaataagc gcaggtttgg aaagcaacat
     4261 tacaattgaa gtggatgctg aaagaacggt aaaatctcaa ttcccgaaag ctggagagca
     4321 ggttgttaaa tcttccatgg ttacactgac actaaattaa tgggacattg gggacggtcc
     4381 tcagcgtccc gccaaagtga caaaaaggac cgtccccagt gtcacaagaa tcaattgcgc
     4441 agtttaatta attatgataa aattgcacat actatcatac atttaaatag gagtatactt
     4501 atgaatataa aaaatctgct caaagaaatg gattatttaa gctatatagg cgaagacgat
     4561 atagaaataa ggggcattac aaacgattca agaaaagtta acagaaatga tatatttgtg
     4621 gcaattaaag gatttacaaa cgatggtcac aaatttattg aaaatgcttt ggaaaatgga
     4681 gcatctgcgg ttatatgcga aaatattcct gaaaatgtaa aaggtaaaag caattttatc
     4741 attgttaaat cagcgagagc atcaatggct aaagcagcca acataatata cggacgacct
     4801 tccgaaaaaa tgaaaattgc cggagtaacc ggaactaacg gcaaaaccag cactacatat
     4861 ttattaaatg gaatatatga ttatttagga gtgaaatcag gaataatagg aacgatgggt
     4921 gttcttattg aaggtaaaaa aatcaaagta gacaacacta ctcctgaagc ttttgatata
     4981 cagcactatt tggcaatgat gcttgaaaag gatattacac attgttgtat ggaggtttca
     5041 tctcatgctc ttgaattgaa cagaattgac gaggttaaga tagatgtggg tattttcacc
     5101 aatgttacaa gagaccattt ggattttcat aaaacaatgg aaaactatta tcaagcaaag
     5161 aaaaagctgt tttatttaac aaaagttaac aatattataa atgtggatga cccttatggc
     5221 ttgagacttt acaatgaact tataaaagaa ggtataaaag caatatccgt aggtattgaa
     5281 aatgatgcgg atattaaagc ttcaaacttg aaaaccacga tgaatggaac aacttttaac
     5341 cttaatgtaa tggggaaaga gaaaacagcc catgtaaata ctcccggaac gttcagtgtt
     5401 cttaattccc ttggagcttt gggagcagca tatgcactgg gtggaaatat tgatgatata
     5461 attgaagctt taaaacaaat aaagggagtt accggaaggt ttgaaatcct ccctaacaag
     5521 cttgattgta ctataatact tgattttgcc catactcccg acggtcttca gaaggtaatg
     5581 gaaactataa atgagtttgc agaaggaaga aaaattgtta tgtttggagc cggcggagaa
     5641 agagatttgt caagacgtgc cccaatgggt gaaattgcag gcaagtactg tgatttaagc
     5701 atacttacat ccgacaatcc cagatttgaa gacccttacg aaatatgtaa ggaaatagcg
     5761 gaaggagtta aaaagtcaaa cggtgaatat aaaataatag ttgagcgcga ccaagccata
     5821 tactatgcta ttgacaagag ccaaaaaaaa gatgtaatat tgttagcggg caaatccaca
     5881 gagccttatc aggatatggg aaccgaaaaa gtaccttatg atgaaactat gcttgctaaa
     5941 agagcaataa aagaagtgga gaaaaaacga ggattgtcat cctgagcgat agctgccttt
     6001 ttgtcatcct gagcgcagcg aaggatctca gggtttataa tatgagattc ttcgagctaa
     6061 agctctcaga atgacacgta gctttttcat agcaatgaca attgatataa ccaatgaatt
     6121 aggagttatt atgcaaggta acggacaaat aataagagta ataatagtat cctttttaat
     6181 agcattgttc ttaggacccg ttgtaattcc ggtcttaaaa cgccttaaag taggacaaag
     6241 tatacgagag gacggacctc aaagccattt agccaagtcg ggtaccccta caatgggcgg
     6301 aattattata atattagcaa ttttaacttc gattatcacc ggaaggctca ttacaaaaga
     6361 agtactggct gtaatattct tcatgctggg ttttgggtta ataggagcca tagatgatta
     6421 taaaaaggtg gtaatgagac aaaatgaagg attaaaaccg tatcaaaaaa tcgtgggaca
     6481 gctgattttt tccgtcctgc ttgcttggta cggctcaaga tacagcgcca tgaaaactgc
     6541 attgatgatt ccattcgtta atatgcacat tgatttaggt gttttgtaca tacccttcat
     6601 tacatttatt gccgtaggta ctgtcaatgc cgtgaatttg accgatggac ttgacggtct
     6661 taacaccgga gtaacattga tagttatggc cgctttcagt ttgatagcaa acagtttaaa
     6721 agaaacaaat caaatatatg aaggaatatc attgatatgt gccgcaacaa gcggtgcctg
     6781 ccttggattt ttaaaacata acgcaaatcc tgccaaagta tttatgggag atataggctc
     6841 attggctttg ggaggtgttg tttcatcagt tgccgtttta tccggcatgg ttttatttat
     6901 acccataatt ggcggcatat actttgttga agcactttca gttattattc aggttttcca
     6961 ttataagaga acaagaagaa gagtatttaa aatggcacct ttgcaccatc atttcgaaat
     7021 gtgcgggtgg aaggaaacaa aggtagtgtc ggccttttgg attgctactc tgatattggc
     7081 atttataggt atttattcaa tataaacaac ataagataat aatgggcatt ataaggaagg
     7141 taaaaaatga atgtacttgt ggtaggatta ggagtttcgg gcattgcctg cataaaagga
     7201 ttaagtaaga caggtgcgaa tatttatgca tttgatgatt caaacgagga gaaattaaga
     7261 gacagattaa aagaagtaga aggtattgaa gttgaatact tcttcggcaa tgaagaaata
     7321 aataaaataa aattcgggaa tttggattat gccataaaaa gccccggtat caaatatgaa
     7381 acaccgatta tcgaaaaact tgttaaaaac aatattgaag taataagtga catagaagcg
     7441 gcttacaggg tgacaaattc acccatcgtt tccataacgg gaactaacgg caaaacaaca
     7501 accacaactc taattggcga aatggttaga gaaagcaaaa aaacctataa ggtaacggga
     7561 aatataggtt ttggaatata ttacgatgca ttaacggcaa aggaagaaga aattcttgtg
     7621 gctgaggtaa gttcttttca gttagcaggt acttcaaagt acaaacctca tataagcgta
     7681 ttaaccaata ttacacctga ccatttagac tatcatcaca cggtagaaaa ttatatagaa
     7741 gcgaaattta aaaatgtaat taatcaagat gaaaatgatt atgcaatatt aaattatgaa
     7801 gacgaaacaa tcaggaatca ttctgataaa ataaaggcaa aaaaaatatt cttcagctcc
     7861 gaaagaattt tggaggatgg aatatattca gaaaacggtc aaatgtttta cagaaccggt
     7921 aacaataaac tatccattat caatacaaag gatatattta taccggggaa acacaattta
     7981 gaaaatgcaa tggctgccgg cggtgctgca atagctcttg gaattgatat tgaagttata
     8041 tcaagagttt taagagaatt taaaggtgtt gagcatcgtc ttgaatttac cggcgaatac
     8101 aagaaggtta aattctataa tgattcaaaa ggaacaaatc ccgatgcatc aattaaggca
     8161 attcaaggaa tcgaaaaacc gattatcttg atagcaggcg gatatgataa gaagtcatcc
     8221 tacgatgatt ttattaaggc atttgacaat aaggtaaaag cattgatact tcttgggcag
     8281 actgccgaag atattcaaaa gtgtgctttg aaatatggtt ttgaaaatat ttacagagtt
     8341 gaaacaatgg atgaagccgt gaagaaaagc tttgaattgg ccgaagaagg agacaatgtt
     8401 gttttatccc cggcatgcgc cagctggggg atgtacccta attatgaagt cagaggcaga
     8461 gattttaaag aaagggtaga atattatgga agaaaccgta agaaaagcta aagccaggtc
     8521 aattgattgg gtattggttt ttataatagt attattggtc ttgtttggat ttttaatggt
     8581 ttacagctcc tcttatcccg acggatatta taaatatggg ggagatcctt tttatttttt
     8641 acgaaaacaa gttattgcag caggagcagg acttgccgga atgatatttt ttgcaaatct
     8701 tccatacata atctacaaaa aattcagtaa gctgatatta ttcacatgta taggattagc
     8761 aatgcttacc attataatag gaattgcatc aaatggcgcc cagaggtgga tagagattgg
     8821 cggaatatca ttgcagccct cggaaataat taaaatcgga gctgtacttt atatggctga
     8881 ttatttcaac agaaaaagaa gtgaattgcc atcctttacc aaaggttttt tgccatcgtt
     8941 gttttatata gtgctatttt gcgggttaat tgctatacag gatgatttaa gcacagctgt
     9001 aatattagcc ggaactctga tggttatgtt tttttgtgca ggagcaagaa tcagccattt
     9061 aatactgttg tttcttcttg cacttgcggg cataatcgca ctgattgcaa tgcagccata
     9121 tcgaatatca cgtataatag tcttttttga cccttttaaa tatcccttgg atgagggtta
     9181 tcaaataata aattcactgt atgccttggg taccggggga ttatttggta tgggagtagg
     9241 aaaaagcaga caaaagtttt ttcatttgcc ggaagcatac aatgacttta ttttctctat
     9301 aataggagaa gagctgggct ttgttggagc ggtctttgta atgctgggat ttgtggtatt
     9361 tgcatggaga ggacttagga tttcaatgaa cacagaagat tttttcggaa gccagacagc
     9421 cttaggcctg actgttatag tactggttca gtttttaata catgttgcgg ttgcaacatc
     9481 ttcgatgcca ccaacgggaa gagcccttcc ctttataagc gcaggtggct catcgataat
     9541 gtttttgctc tgctccatgg ggattttgct taatatatcg aaatattcca atacttacag
     9601 gtcatagtta tgtcatcctg agcgaagcg
//
LOCUS       DAOSHP010000066         9523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_6116_length_9523_cov_7.938741, whole genome shotgun sequence.
ACCESSION   DAOSHP010000066 DAOSHP010000000
VERSION     DAOSHP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6116_length_9523_cov_7.938741"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..1423
                     /locus_tag="PLB33_07000"
     CDS             356..1423
                     /locus_tag="PLB33_07000"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HOG63309.1"
                     /translation="MKKRWFFLWLIPVSVLINLFVKNNANYAEFYAVNIYPFFVKTVG
                     RLSFITRSSAAELIIFVLIVTIIILTALVTVGSIKYRTFEYIKKYIFGVASIISVVYF
                     LFVLFCGINYYRYEFTHYSGLEIQMSSKQELIELCELLIGDANECRAKLETHEGTAKL
                     SDKSYYTTAERAGDFMEGLSEEYEILKGNFSAPKPVRQSKIMSYFKITGVFFPYTFEA
                     NVNVHIPPYQIPFVMLHELVHLRGFMREDEANFISYLACIKSGYDDFYYSGTMSALTH
                     SMNALYSADYDEFVRLYNLYSDDVRKDIDFSIRYWKQFDTKVADAAKKVNDIYLKAHN
                     QQDGVRSYGRMVDLLLAYYRQ"
     gene            1484..1792
                     /locus_tag="PLB33_07005"
     CDS             1484..1792
                     /locus_tag="PLB33_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOG63310.1"
                     /translation="MAEIKCTVTNCYFNKKLGCTAPALTVNGRNADESRSTSCHTFIE
                     QKPGLRSSVEEPKSNTDILCAATKCIYNESERCEANSIIVNGKNASVPEETCCSTFRS
                     "
     gene            1925..4153
                     /locus_tag="PLB33_07010"
     CDS             1925..4153
                     /locus_tag="PLB33_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOG63311.1"
                     /translation="MMKNTYKIILLTLILILCFTLTSFAQDEIKVYVDNERVDFDVEP
                     IIENGRTLIPLRGVFEKLGARVDWNKNLLEVVIKDDNNEIEMILGKDKVMVNGIIKDI
                     DVPTRMINSRTFAPLRFIAENLGHTVRWDGTTSSVYITKNDTFPVEKGISTVGTKENL
                     IALLEYNSKLHNYIWRGRDIVVDFAEDSITEAQEAPKAEAESAAPGMDSSDTNVQVEG
                     VQEGDIIKNDGKYIYINTNRGLKIIDSNPDAPKIVYTVPVPENTSISEIFTAGNKLIV
                     IGQNNLFYIMDKSAIDSIDMLEPSIMPPRYYQDRTDVLIYNIENIEKPVLEREYLFDG
                     NYLSGRVIDDSFYLINNKYLNLGYGYYDNKDIDIPLPYYTDVLNYEDYKFGYDEIKYF
                     PNYIDTRYMYIIGIDLNDTKSKPDVDVYLGGTDTIYVSKENLYAAVADYSYNYIVRPL
                     EEFSPEYTTSTVIYKFELKDGKIGTAVQGEVPGTIINQFSMDEHNNTFRIATTTGDMW
                     RNTSKNNVYILDEDLKVLGKLEGLAEGERIYSTRFAGDRMYMVTFRQVDPLFVIDTSN
                     PRAPELKGMLKIPGYSTYLHMVDENHILGFGYDTEVNQWGGTVNGGLKISMFDVSNVN
                     KPKETFTEIIGKSGTYSELLYNHKALMFSLNKGLMAFPVNRTAENYKSDFSGAYVYNV
                     SNDSISLKNTITHKESEFDSYGDNIKRIIYIGDYLYTFSENKMQIHGINTNKKVNELI
                     IK"
     gene            4212..4802
                     /gene="yfbR"
                     /locus_tag="PLB33_07015"
     CDS             4212..4802
                     /gene="yfbR"
                     /locus_tag="PLB33_07015"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HOG63312.1"
                     /translation="MKKSNFFAFISRMKYIDRWGLMRNINKENVSEHSLDVAVIAHAL
                     AVIQKNRLNMDVNPEKTALYAIYHDVSEIFTGDMPTPVKYYNSVIKSAYKDVELSVNE
                     RLLNMLPEDFKDEFKDILIPKEEEKDIWRTVKAADKISAYIKCLEEEKSGNKEFLKAK
                     QSLLSTIRNMNMSEVKIFMDEFLEGYSLTLDEMEMS"
     gene            complement(4819..>5391)
                     /locus_tag="PLB33_07020"
     CDS             complement(4819..>5391)
                     /locus_tag="PLB33_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOG63313.1"
                     /translation="MPQAYEIGGFFIVTLGLVIGLIIVFYANDLIPLNKLKQYKGKKL
                     SYIKMSLIIIISISLHNFPEGVAVGSSYVYSDNLGVKIGLLIAAHDIPEGISVGVPLI
                     MAGLSPVMVVFLTLLTGIPTALGAVFGALVGGVSDYFIAFCLSAASSSMLYVSANELI
                     PEANLLYNGKSSSVLLLLGFMCGCYISFAV"
     assembly_gap    5392..5491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5613..6674
                     /locus_tag="PLB33_07025"
     CDS             5613..6674
                     /locus_tag="PLB33_07025"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOG63314.1"
                     /translation="MMENKINTKVVKVDPENIDCNIIKEAADIIKRGGLVVFPTETVY
                     GIGADALNDEAVDKIFKAKGRPQDNPLIVHIAEFHELYDLALEVPEKAELLAEKFWPG
                     PLTMILYKKKILSDKITAGLNTAAIRLPENKIALALIRESKKPIAAPSANTSGSPSPT
                     EASHVIEDLMGKVDMIIDGGSTYIGLESTVVDMTSSVPMILRPGGVTAEDIISVLGEC
                     SYDPAIIKSEEIVIPKSPGQKYRHYSPKAKVILYKGKIERIVEKINEDYDKFTSLGLK
                     TGIMSTAQTEEFYKGKINICIGDRTKLLTISSNLFKGLRDFDHMGVNVILAEAVDEKG
                     LGKAIMNRLGKSASETVEV"
     gene            6676..7164
                     /locus_tag="PLB33_07030"
     CDS             6676..7164
                     /locus_tag="PLB33_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOG63315.1"
                     /translation="MNILFVCTGNTCRSPMAEGIFKDMLMKNNINNISVSSAGISVFP
                     GEHASEKAIKALKEKGIDITGHRARQLLDEINNVDLILTMTLSHKMIISDYLKNETDE
                     KSPRLFTLKEFAAKLSGEKLTDTDIVDPYGKNYNFYKNSRDEIEKELIKILKNLDKLE
                     GI"
     gene            7161..7616
                     /gene="rpiB"
                     /locus_tag="PLB33_07035"
     CDS             7161..7616
                     /gene="rpiB"
                     /locus_tag="PLB33_07035"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOG63316.1"
                     /translation="MKIVLASDHGGFELKEAIKKHLIKKGYDINDIGVYDTKSVDYPD
                     YGKRAALMVADKEADRGIIVCGTGIGISIAANKIKGIRCALCTNEYMARMSRMHNNAN
                     MLALGGRVTGIGLATDMVDVWLSTEFEGGRHENRVNKIMDIEISKEEEK"
     gene            7616..8644
                     /locus_tag="PLB33_07040"
     CDS             7616..8644
                     /locus_tag="PLB33_07040"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOG63317.1"
                     /translation="MTANLIAFISAVVISFVMTPWARRLAIKVGALDIPKEARKVHNK
                     PMPYFGGLAIYVSIIACMFVYMPHTVTNYYIMAGATVIVLAGVVDDMYGMPAKIKLLI
                     QIIAALIAIRGGVQIHFITNPLSETGMSYLFDWLSFLITLFWLVGITNTINLIDGLDG
                     LASGVASIAATTLLFTAARLGHDFIVMQCAIIAGASLGFLPFNFNPAKIFMGDTGSLL
                     LGYMLAVTSVSGMVKSVAVVALAVPVFALGLPIFDTTFAIIRRYINKKPIMEADKEHL
                     HHKLMGVGLNQRQTVLIMYFISMLLGIVSVIIADADPFTGLTVATAVVIIAFIFAKKA
                     GLFVRKEQ"
     gene            8730..>9523
                     /gene="wecB"
                     /locus_tag="PLB33_07045"
     CDS             8730..>9523
                     /gene="wecB"
                     /locus_tag="PLB33_07045"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOG63318.1"
                     /translation="MKVLVVFGTRPEAVKMAPIVKALKKDEDNFISKICVTAQHRDML
                     DQVLRIFDIEPDYDLNIFENGQTLTQITCRALTGLEEVIEDFKPEIILVQGDTTTVFA
                     GALAAFYHQVKIGHVEAGLRSGNLYSPYPEEANRMMTGVLTDFHFAPTETSKKNLIRE
                     GYDENKIFITGNTSIDALRWVIDDNYKFEEEIINNIDFENKRVILLTAHRRENIGKPM
                     ENIFSALKDIAEENQDVEVIYPMHLNPKVREIAHRIFGNLENVHLI"
BASE COUNT         3312 a         1383 c         1973 g         2755 t
ORIGIN      
        1 ccagttgatg ttttagaaaa agctgtaaca gatgcaggat atgaagtagt ttaaaaggca
       61 ggggacttac tctgatacaa acccgagaaa tctcgggttt tttcatgcat aaggtatgta
      121 ctatttgtca tcctgagggt aagtgaagga tatcattgag attcattggt cttgacattt
      181 attgatataa ataatcagtg taattagtac tattatatga taaaatgaat acaatcaatg
      241 aagcaaagac ctgtgtcttt gcactgatgt attcattgta tcggtgtgaa gtaagccgat
      301 tttgcggctt gcacttatga taaaatttta atatattttt tggatgaggt agaaaatgaa
      361 aaaaagatgg ttttttcttt ggctgattcc tgtttctgtt ttgattaact tatttgtaaa
      421 aaacaatgcg aattatgcgg aattctatgc agttaatatt tacccgtttt ttgtgaaaac
      481 tgtaggcaga ttgtcattta ttacccgcag ctcagcagcc gaattaataa tttttgtatt
      541 aatagtgaca ataataatac ttacagccct tgtcacagta ggaagtataa aatatagaac
      601 atttgagtat ataaaaaagt atattttcgg ggttgcttca attatttctg tggtgtactt
      661 tttgtttgtt ttattctgcg gaataaacta ttacaggtat gaatttactc attacagcgg
      721 cttagaaatc caaatgtctt caaagcagga attgattgag ctttgtgaat tgctgattgg
      781 agatgcaaat gagtgcaggg caaagctcga aacccatgaa ggaaccgcaa agttatcaga
      841 taagagttat tatacaacag cagagagagc cggagatttc atggaaggtc tgtcggaaga
      901 atatgaaata ttgaaaggaa atttttctgc accgaagcct gtgagacagt caaagattat
      961 gtcctacttt aagataacag gagtcttttt tccttataca tttgaagcta atgtaaatgt
     1021 ccatatacct ccctatcaga ttcctttcgt aatgcttcat gaattggtgc atttaagggg
     1081 atttatgagg gaagatgaag caaacttcat atcatattta gcatgcatta aatccggata
     1141 tgatgatttc tattacagcg gtaccatgtc agctttgact cattcaatga atgctcttta
     1201 ctcggcggat tacgatgaat ttgtaagatt gtataatttg tattcggatg atgttagaaa
     1261 agatatagat ttctcgataa ggtattggaa acagtttgac acaaaggttg cagatgcagc
     1321 caagaaagta aatgatattt atttaaaagc gcataatcag caggatggtg taagaagtta
     1381 cggaagaatg gtggatttgc ttttggcata ttacagacaa taggcataat taaacatctt
     1441 tgggataaaa taaagaaaac tcattgatga aaggagaaag acaatggcag aaattaagtg
     1501 tacggtaaca aattgttact ttaataaaaa attaggctgc acagctccag ccttaaccgt
     1561 taacggaaga aatgccgatg aaagccgctc aacaagctgt catactttta ttgagcagaa
     1621 gccgggatta agaagttctg tagaagagcc aaaaagcaac acagatattt tatgcgcggc
     1681 aacaaaatgt atttataatg aatctgagcg ctgtgaagca aatagtatta ttgtgaacgg
     1741 taaaaatgca agtgttccgg aggaaacttg ctgcagtaca ttcagaagtt agattagagg
     1801 aaggggggac accaataaag atggcagatg aaaccatctt tttatatgtt tataattaga
     1861 attatttggt ataattaagg aacaagatat tgctaaaata cgtcttatga aaagagaggt
     1921 gaatatgatg aagaatacgt ataaaattat tttattaacg ttaattttaa ttttatgttt
     1981 tactttaaca tcatttgctc aagatgaaat aaaagtttat gttgataatg aaagagtgga
     2041 tttcgatgtt gagcctatta tagaaaatgg caggacattg ataccattga ggggtgtttt
     2101 tgaaaagctg ggagccaggg tagactggaa caaaaacctt ttggaagtgg ttattaagga
     2161 tgacaacaat gaaatagaaa tgatattagg caaggataaa gtaatggtta acggtattat
     2221 caaggatatt gatgtgccta caagaatgat aaacagcagg acctttgccc cccttagatt
     2281 tatagccgaa aacttagggc atactgttag gtgggatgga acaacaagct cggtatacat
     2341 aaccaaaaat gatacttttc ctgttgaaaa aggcatttca actgttggaa caaaggaaaa
     2401 tttaattgct ttattggagt ataattcaaa actccacaat tatatctgga ggggcagaga
     2461 tattgttgtc gattttgctg aagattccat tactgaagct caagaagcac caaaagccga
     2521 agcagaatct gctgctcccg gcatggacag ctcagataca aatgttcaag ttgaaggtgt
     2581 tcaggaaggg gatattatta aaaatgacgg aaaatacatt tatattaata caaacagagg
     2641 cttaaaaata attgattcaa atcctgacgc cccaaaaata gtttatacag ttcctgtccc
     2701 cgaaaataca agtatcagtg aaatttttac ggcaggaaat aagctgattg taatcggtca
     2761 gaacaacctt ttctatatta tggataaatc ggcaattgac agcattgata tgcttgagcc
     2821 ttccataatg ccgccgagat actatcagga cagaaccgac gtattgattt ataatataga
     2881 aaatattgaa aaaccggtac ttgaaaggga atatcttttt gacggcaact atttatcagg
     2941 cagggttatt gatgacagtt tttatttgat aaacaacaag tatcttaact taggatacgg
     3001 ttactacgat aataaggata ttgatatacc tcttccatat tatactgatg tgctgaatta
     3061 cgaggattat aaatttggat atgatgaaat caagtatttt ccaaattata ttgacacaag
     3121 atatatgtat ataataggaa ttgatttaaa cgatacaaaa tcaaaaccgg atgttgatgt
     3181 gtacttaggc ggcacggata caatttatgt ttcaaaggaa aacttgtatg cggcagtagc
     3241 cgattattca tataattata tagttcgtcc gcttgaggaa tttagccctg agtatactac
     3301 tagtacggta atctataaat ttgaattgaa agacggcaaa ataggaactg ctgtccaagg
     3361 tgaagttccg ggaactataa ttaatcaatt ttcaatggat gagcataaca acacctttag
     3421 aattgcaaca actaccggtg atatgtggag gaatacatcc aaaaacaatg tatatatttt
     3481 ggatgaagac ctaaaagttt tgggaaaatt ggaaggtttg gctgaaggtg aaagaattta
     3541 cagcacacgc ttcgccggag acagaatgta catggttaca ttcaggcagg ttgacccctt
     3601 gtttgtaatt gacacatcaa acccaagagc tcccgagtta aagggaatgc ttaaaatacc
     3661 gggatacagc acatatcttc atatggttga tgaaaatcac attttaggat ttgggtatga
     3721 tactgaagta aatcaatggg gcggaaccgt aaacggcggc ttaaaaattt caatgtttga
     3781 tgtttcaaat gtgaataaac caaaagaaac atttacggaa ataataggga agtcgggaac
     3841 atattcagag ctcttgtaca atcacaaggc attgatgttt tcattaaata agggattaat
     3901 ggcatttccc gtaaacagaa cagcagaaaa ttataaaagc gactttagcg gagcatatgt
     3961 atataatgtt tccaatgaca gcataagttt aaaaaataca ataactcaca aagaatctga
     4021 atttgattct tacggagata atatcaaaag gataatttat atcggtgatt atttgtacac
     4081 attttctgaa aataaaatgc aaattcacgg catcaataca aataagaagg ttaatgagtt
     4141 aataattaaa taaataaagg tgccaggcac ctttttgggt gcctggcacc aatataccta
     4201 tgggagagct tatgaaaaag tctaattttt ttgcatttat atcaagaatg aagtatattg
     4261 accgctgggg cttaatgaga aatataaaca aggaaaatgt atctgagcac agtcttgacg
     4321 ttgctgtaat tgctcatgca ttggctgtaa ttcaaaaaaa ccggttgaac atggacgtaa
     4381 atccggaaaa gactgccctc tatgcaattt atcacgatgt atcggaaata tttacgggag
     4441 atatgcccac acctgttaaa tattacaatt ctgttattaa gtctgcctat aaagatgtgg
     4501 agttgtcagt aaatgaaagg cttttaaata tgcttccgga agattttaaa gatgagttta
     4561 aggatatctt aattcctaag gaagaagaaa aagatatatg gaggaccgta aaggctgccg
     4621 ataaaataag tgcatatatt aagtgtttag aagaagaaaa gtccggcaat aaggaatttt
     4681 taaaagcaaa gcaatcattg ctttcgacaa taagaaatat gaatatgtct gaagtaaaaa
     4741 tatttatgga tgagttcctg gaaggttaca gccttacctt ggatgaaatg gagatgtcct
     4801 aaccctattt ttaaataatc ataccgcaaa ggatatataa catccgcaca tgaaacctag
     4861 aagcagcaat acagagctgc tttttccgtt ataaagtaag tttgcttcag gaattaattc
     4921 attggctgac acataaagca tgctgcttga tgcagcggaa agacaaaaag ctatgaagta
     4981 atccgataca cctcccacca aagcaccgaa tacggccccc agagcagttg gaatacctgt
     5041 aagaagagtc aaaaaaacta ccattacagg agataatcct gccataatca aaggaacacc
     5101 aacacttata ccttccggta tatcgtgagc tgcgatgagt aatcctattt ttactcctaa
     5161 attatctgaa tacacatagc ttgagcccac cgcaacacct tcggggaaat tgtgcaagct
     5221 tatactgatt attataatta agctcatctt gatataactt agcttttttc ctttatattg
     5281 ttttaattta ttcaagggaa taagatcatt tgcatagaaa actattataa ggcctattac
     5341 aagacctaag gtaactataa agaaccctcc gatttcatat gcttgaggca tnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntttaagaaa ttaaaaaatt attaatcagt
     5521 aaaattcttg tacataaagt tattgtagta tataatatta cgagaacatt ccttcatggg
     5581 atgacggcaa atcgccaagc aagagaaggt atatgatgga aaataaaata aatacaaagg
     5641 tagtaaaggt tgaccctgaa aatatagatt gtaatattat taaagaagct gcagatatta
     5701 ttaaaagggg aggtcttgtt gtttttccta cggaaacagt ctacggaatc ggtgctgatg
     5761 cattgaatga cgaagcagtc gataaaatat tcaaggcaaa gggacgtcct caggacaatc
     5821 ctttaattgt acatattgca gaatttcatg aattgtatga tttagctttg gaggtgccgg
     5881 agaaggcaga gcttttggca gaaaaatttt ggccgggacc actaacgatg attttatata
     5941 aaaagaaaat attatcagat aaaattactg caggattaaa tacagctgcc atacggctgc
     6001 ctgaaaataa aattgcactt gctttaataa gagaaagcaa aaaacctatt gcagcgccca
     6061 gcgcaaatac atcgggcagt ccaagtccta cggaagcttc tcacgtaata gaagatttaa
     6121 tgggaaaggt ggacatgata attgatggag gaagtactta tatcggtctt gaatcaactg
     6181 ttgtggatat gacaagctcc gtgcccatga tattaagacc gggcggagtg acagcagagg
     6241 atataattag tgttcttggt gaatgcagtt atgaccctgc aattataaaa agcgaagaaa
     6301 tagttattcc aaaatcaccg ggtcagaaat acaggcatta ttctcccaag gcaaaagtta
     6361 ttttgtataa gggtaaaata gaaagaattg tagaaaaaat caatgaagat tatgataaat
     6421 tcacgtcttt gggattaaag acggggatta tgtcaactgc tcaaacagaa gagttttata
     6481 aggggaaaat aaatatttgc attggagaca gaacaaaact gcttacaatt tcgtcaaact
     6541 tatttaaggg tttaagggat tttgaccaca tgggagtaaa tgtaatactt gcggaagcag
     6601 ttgacgaaaa aggtctcggg aaggccataa tgaacaggct cggtaagtca gccagcgaaa
     6661 cagtcgaggt atagaatgaa tattttgttt gtttgtacgg gcaatacatg cagaagccct
     6721 atggcagaag gtatttttaa ggatatgctt atgaagaata atattaataa tattagcgta
     6781 tcttctgccg ggataagtgt ttttcccgga gagcatgcca gtgaaaaggc aataaaggct
     6841 ttaaaagaaa agggaattga tataacaggg cacagagcaa gacagctgct tgatgagatt
     6901 aataatgttg acctgatatt aacaatgacc ttatctcata aaatgattat ttctgattat
     6961 ttaaaaaacg agacagatga aaagtctcct cgtcttttta ccttgaaaga atttgctgca
     7021 aaattaagcg gtgaaaaatt gacagatact gatattgtcg acccttatgg caaaaattat
     7081 aatttttaca aaaatagcag agatgaaatc gaaaaagaac taataaagat actcaaaaat
     7141 ttagataagc tggagggaat atgaaaatag tattggcaag cgaccatgga ggatttgaat
     7201 taaaggaagc aattaagaaa catttaataa aaaagggcta tgatataaat gatataggtg
     7261 tttatgatac aaaatccgtt gattaccctg attacggtaa gagggctgcc ttgatggtag
     7321 ctgataaaga agccgataga ggtattattg tttgcggaac cggaataggt atttctattg
     7381 cagcaaacaa aattaaagga ataagatgtg ccttgtgcac aaatgaatat atggccagaa
     7441 tgtcaagaat gcacaacaat gcaaacatgc ttgcattagg cggcagagtt accggtatag
     7501 gtcttgcaac ggatatggtt gatgtttggc tttctactga atttgaaggc ggaagacatg
     7561 aaaacagggt aaacaaaatt atggatatag aaatttctaa agaagaggaa aaataatgac
     7621 tgcaaattta atagctttca tatcagctgt tgttatatca ttcgtaatga ctccatgggc
     7681 aagaagacta gcaataaagg tgggagcatt ggatataccc aaggaagcaa gaaaagtcca
     7741 caacaagccg atgccttact ttggaggact tgcaatatat gtatcaataa ttgcatgtat
     7801 gtttgtttat atgcctcaca ctgtcaccaa ctattatatt atggcagggg cgacagtaat
     7861 tgtgcttgca ggtgttgttg atgatatgta cggcatgcct gcaaaaatta aactcttgat
     7921 acagataata gcagcattaa tagcaataag aggcggcgta cagatacatt ttataacaaa
     7981 tccgctttcg gaaacaggta tgagctactt gttcgattgg ttatcttttc ttattacatt
     8041 attctggtta gtgggtatta caaatacaat aaatttaatt gacgggcttg acgggcttgc
     8101 ttcaggggtt gccagcattg cagccacaac tcttttattt accgcagcaa gattgggaca
     8161 tgacttcatt gtgatgcagt gtgcaattat tgcaggtgct tcactggggt ttttgccttt
     8221 taattttaat ccggcaaaaa ttttcatggg agatacaggc tccttgttgc tggggtatat
     8281 gcttgcggtt acatctgttt cgggtatggt aaagagcgtt gcagttgttg cattggctgt
     8341 cccggtgttt gcattaggtc tgcctatatt tgatactacg tttgcaatta taagaagata
     8401 tataaataaa aaacctataa tggaagcaga caaggaacat cttcatcata agcttatggg
     8461 agtcggcctt aatcagcggc agacagtctt aataatgtat tttataagta tgcttttggg
     8521 aatagtttcg gtaataatag cagatgcaga ccccttcacc ggtttgacgg ttgctactgc
     8581 ggttgttata attgcattta tatttgcaaa aaaagcggga ctgtttgtaa ggaaagagca
     8641 ataagtgtca tcctgagcgt agcgaaggat ctcatgagat tcatcggact gatatattgt
     8701 tgcggttaat aggaggaaaa atggataaaa ttaaggtttt ggtagtgttc ggaacccgtc
     8761 cggaggctgt aaaaatggct ccaattgtga aggctcttaa aaaggatgag gataatttca
     8821 tatctaaaat ttgcgtaact gcccagcaca gggatatgct tgaccaagta ctgaggattt
     8881 ttgatattga gcccgattat gatttaaata tttttgaaaa cggtcaaaca cttacacaaa
     8941 ttacatgcag agcattaaca gggttagaag aagtaataga agattttaaa cctgaaataa
     9001 tactggtaca gggagataca acaacggtat ttgcaggagc tcttgctgct ttttatcatc
     9061 aggttaaaat aggtcatgtg gaagcaggac ttagaagcgg caacctgtat tcgccttatc
     9121 ctgaggaagc aaatagaatg atgacaggtg tattgaccga ttttcatttt gctccgacag
     9181 aaacatctaa gaaaaatctc ataagagaag gctacgacga aaataaaata tttataaccg
     9241 gaaatacttc aatagatgct ttaagatggg ttatagatga taattataaa tttgaggaag
     9301 aaataatcaa taatatagat tttgaaaata aaagggtaat actcttgact gctcacagaa
     9361 gagaaaatat cggcaaaccg atggaaaata tattttctgc tctgaaggat attgcagaag
     9421 aaaatcagga tgtagaggtt atatatccaa tgcacttaaa tcctaaggta agagaaattg
     9481 cccatagaat atttggcaat ttggaaaatg ttcatttgat tga
//
LOCUS       DAOSHP010000067         9425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_6189_length_9425_cov_9.336073, whole genome shotgun sequence.
ACCESSION   DAOSHP010000067 DAOSHP010000000
VERSION     DAOSHP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6189_length_9425_cov_9.336073"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      59..158
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            326..1630
                     /locus_tag="PLB33_07050"
     CDS             326..1630
                     /locus_tag="PLB33_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOG63319.1"
                     /translation="MKQFFKLEENNTNVKTEVLAGITTFMTMAYILIVNPIILSETGM
                     DFGGIFTATALSSLIATLMMALYANYPFALAPGMGLNAFFAFTVVLGPMGKSWEFALT
                     AVLIEGIIFILLSFIKAREAIFESIPLNLKNAVSVGIGIFISFIGLSGAGIVKAGNGT
                     LVALGELTSGAPLLAIIGIIITGLLLIKNVKGALLIGIVITTIIGIPMGVTILPENFS
                     TIVSMPPSLKDVAFKFVGFDQIFSFEMLVVVFTFLFVDIFDTVGTLAGVATKANMLDE
                     EGKLPRVGAALMSDAVGTVVGACLGTSTVTTYVESASGVAEGGRTGMTSLVTAAMFGV
                     ALFFAPLFTLVPSAATAPALVIVGLFMMSPITKINFNDYTEAIPAFLTIVMMPFAYSI
                     AEGIVFGMVSYVILKAISGKFKDISLVMWILSVLFVLRFIFI"
     misc_binding    1774..2002
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2112..2996
                     /gene="cysK"
                     /locus_tag="PLB33_07055"
     CDS             2112..2996
                     /gene="cysK"
                     /locus_tag="PLB33_07055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOG63320.1"
                     /translation="MINTLDLIGNTPVIKLPGENIYLKMEKFNPGGSVKDRAALGMIE
                     DAEAKGYLNKNSIIVEPTSGNTGIALALIGRLKGYKVIIVMPETMSMERRNIIEAHGA
                     TLILTEGQKGMSGAIARAHELLKDDNNYFMPDQFNNPANPRIHYDTTGQEIIRQLNDI
                     DIFTAGVGTGGTLTGVSKRLKEYNKNIITAGVEPSKSPVLSGGSPSPHKIQGIGAGFV
                     PGTYNGSFVDEIITITDEEAIDTAVEVSAKIGMLIGISSGANVAAARKLAQKYGSNKK
                     ILTISPDGGEKYLSVVKY"
     gene            3014..3571
                     /gene="cysE"
                     /locus_tag="PLB33_07060"
     CDS             3014..3571
                     /gene="cysE"
                     /locus_tag="PLB33_07060"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOG63321.1"
                     /translation="MIKFIKSIIEDIDSVFDRDPAARSRMEVLLLYPHIKALIAYKIS
                     HKLYLKRRYFLARLISNIARHRTGIEIHPGATIGKGLFIDHGMGVVIGETAVVGNNVT
                     MFHGATLGGTGNEKGKRHPTVGDNVVIGASAKILGNIYIASGTKVGANAVVLNDTNPN
                     STLVGIPAREVKKGNKIINLSEYAI"
     gene            complement(3700..4725)
                     /locus_tag="PLB33_07065"
     CDS             complement(3700..4725)
                     /locus_tag="PLB33_07065"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOG63322.1"
                     /translation="MKKLLVISIVLAVILTACNNIAVPPEEPDNGETVSIEEYYPIKE
                     NTKYSYEGEGNEFATYVAFIDYVSDNRIQNRTNNGGTETVNVYEIKDDQLTLLYSRGE
                     TYFRQNFLNKEYGEGKVLLKGPLEEGNSWAYDENTTATITDLSKEIVTTLGNYQAIEV
                     TLEGEQGKTVNYYAKDVGLIKTISSGEGYEVSSTLSSIENNIPLIQTITLFYPNTDGS
                     IDTIDVQIEFNTNDEPKDIIEEVVKELSVYEILSPNTIINELYFDEEENSVHIDLSDD
                     FITEMNAGSSFENLILQSLTNTLGNYYGVQEVYLTIDGGPYESGHIRIEEGEPLLVDY
                     DNVNTRE"
     gene            complement(4867..5676)
                     /locus_tag="PLB33_07070"
     CDS             complement(4867..5676)
                     /locus_tag="PLB33_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63323.1"
                     /translation="MDAMLTLKNVSAGYNGIDVINNISLCVKEGENLCIIGPNGCGKT
                     TLIKAISGIIPHTGIIKIGGTDINNMKREQVAKKIAVMSQISTIYFSYSIYETVLLGR
                     YLHMKGRTFKEPSAKDREYTDKCLKAVGLFNIKHKQISTLSGGQLQRVYLARTLAQEP
                     NIILLDEPTNHLDLKHQTELIDFLKDWSKYEGHSVIGVMHDLNLAIKLSDNMLVLDNG
                     NIAAYGSTNEIISSNLLNEVYDMNVTEYMVDSLKHWESFGLCHPEPKRRIS"
     gene            complement(5673..6698)
                     /locus_tag="PLB33_07075"
     CDS             complement(5673..6698)
                     /locus_tag="PLB33_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOG63324.1"
                     /translation="MKIKFKIILSIIFSIIVVIMGVGLGSVNVPPSHIISVILNKIFS
                     APLPSDIEGTTAISIIWKLRLPRTLLAFLVGGAVAVSGSVMQSVLKNPLASSYTLGVS
                     SGASLGAALVIVTGFNIPFLGMFTLPVTGLVFGMATVFLAVKFAAMMDKNLETNTIIL
                     VGMVFSLFVNAILTVITAMARDHLAQLTYWQMGSFSLKDWSSVIILFPIVLIGITVLQ
                     NYSKEMDIMTFGEEQAFTMGVDVHKVKWILLSISAALTGSAIAFVGVIGFIDLIAPHV
                     VRKIFGSNHKTVIPMSVFFGGSFMVLCDLIARTIISPSELPVGAITALIGAPFFAYVY
                     FSRRKGL"
     gene            complement(6695..7636)
                     /locus_tag="PLB33_07080"
     CDS             complement(6695..7636)
                     /locus_tag="PLB33_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63325.1"
                     /translation="MKRNSILALTLICIMALLTACAENTEVKSPQPEDINITLPTKDR
                     AGNEISIPNEINRIISISPANTEILIELGFGDKIIAVDEYSKDIEGLPNNIQLFDMMA
                     PDAEQMVALEPDIIYTTGMVMAEGNDPYKPIRDMGVCVAYIPASLSIEGIYEDILFFA
                     KSLGVEKKGIDLVNSMKSKIKEIEEIGKSIENKKTVYFEIAAAPTLYSFGKGVFLNEM
                     IEIIGAENVLSDHDWWISVSEETVIASDPDVIITNVDYIENPAEEIKSRSGWENMKAV
                     KNNDVYYVDNYATSHCNHNIITGLEQMARAIYPDIYQ"
     regulatory      complement(7838..8024)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8115..9287)
                     /locus_tag="PLB33_07085"
     CDS             complement(8115..9287)
                     /locus_tag="PLB33_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOG63326.1"
                     /translation="MDLREEALKLHKDNKGKIEVISKVRVENAKDLSLAYTPGVAEPC
                     KEIHANPELIYEYTNKGNMVAVVTDGSAVLGLGNIGSRAGMPVMEGKAVLFKSFANVD
                     AFPICIESQDVDIVVQTVKQIAPSFGGINLEDIAAPRCFEIEERLKKELDIPVFHDDQ
                     HGTAVVVLAGIINALKAVGKKIENVTAVVSGAGAAGIAISKLLLSSGINDVIICNSRG
                     ILDKEDERLDPIRKEMALKTNKANKKGALSDALKGADIFIGVSAPNIVTKEMIESMNE
                     NSIAFAMANPIPEILPEVALAGKVKVMGTGRSDFPNQINNVLAFPGIFRGALDVRAKE
                     INEEMKLAAAHAIAELVTEEELNEEYIIPKAFDKRVAKAVARAVSEAAIKTGVNRI"
BASE COUNT         2915 a         1738 c         1658 g         3114 t
ORIGIN      
        1 agcgttttaa gtatacatca ttttcctatt gacatattta aaacatattg gtattatcaa
       61 tatgaaaata aatatagctc atatatatct cattatatgg ttgagatgtt tctacaaaat
      121 gacctaatca tttggctatg agtgaaagtg tatctagggt tccgaagatt gcttgctgga
      181 ccgagcggta cagatacttt aagtattaca ccgaagggat aaaagcccag tcgggtaggt
      241 ttcacttcct acctcgacag gctttattta ttttttgtgg ttaattcata tgataaaata
      301 ttaaaaaatc aggaggaata atactttgaa acaatttttt aagcttgagg aaaataatac
      361 taatgtaaaa acagaagtat tagcaggcat tactactttt atgacaatgg cctatatttt
      421 aattgttaat cctatcattc tatcagaaac cggaatggat tttggcggta tatttactgc
      481 tacggcctta tcatcactta ttgcaacact tatgatggcc ctctatgcca actatccgtt
      541 tgccttagcc cccggtatgg gacttaatgc attctttgca ttcactgttg tattgggacc
      601 aatgggcaag tcatgggaat ttgctctaac tgctgtatta attgagggaa ttatatttat
      661 tttactatcc tttataaaag ccagagaagc aatatttgaa tctatacctt taaatttaaa
      721 gaatgctgtt tccgttggaa taggtatatt tatatctttt ataggtttat ccggtgcagg
      781 aatagtaaaa gcaggcaacg gtacgcttgt tgctctgggc gaattgacat caggtgctcc
      841 attattggct attataggaa tcattattac aggattattg ctgataaaaa acgttaaggg
      901 agcattgtta ataggaatag ttataacaac aattattgga attccgatgg gagttacaat
      961 actgccggag aatttcagta caattgtaag catgcctcct tcacttaagg acgttgcatt
     1021 taaatttgta ggatttgacc aaatattttc ttttgaaatg ctggtggtag tattcacatt
     1081 cctctttgta gacatttttg atactgtcgg aacattggca ggcgttgcca caaaggcaaa
     1141 tatgctggat gaagaaggga aattgccgag agtaggagct gctctaatgt ctgatgctgt
     1201 tggtacggtt gttggtgcat gtttaggaac aagtactgtt acaacatatg ttgaaagtgc
     1261 ttcaggcgtt gctgaaggcg gaagaacagg aatgacatcg cttgttactg cagcaatgtt
     1321 tggagttgca ctgtttttcg ctccattgtt cacattggtt ccatcagctg caactgcacc
     1381 tgcattggtt attgtaggtt tgtttatgat gagccctata accaaaataa atttcaacga
     1441 ttatacagaa gcaataccgg ctttcttaac aattgttatg atgccttttg catatagtat
     1501 tgcggaagga attgtatttg gaatggtatc atatgtgatt ttgaaagcta tatccggcaa
     1561 attcaaagat atatctttgg ttatgtggat tttatcggta ctatttgttt taagatttat
     1621 atttatttaa agtaaaagat aaaacagcct gccgttggag ataaacggca ggttttttta
     1681 tttttaaata gattaaaaaa atttttagaa aaagtgttgc attttataaa gcagtataat
     1741 ataataaaga aaatataccc aactaaatta gtaggaaata cggcgaaaaa ggaaagtagc
     1801 atataacgaa tttacagaga gagcatctca aggctggaag gatgcttatt ggaagtatat
     1861 gtgaagtgcc ctttggagta cagcaccgaa ttaagtaggc tgctgcggaa ccatccgtta
     1921 tagtggagta gcatgatggt gctatataag ggggctatag agccaattag agtggaaccg
     1981 cggatttcaa tccgtctctt tgcagaggcg gattttttgt atgtcctaac ctgcgaatac
     2041 cacttatgca tatatattcc gtttgtagct atattgatta tcgatttagt ggaaaagaaa
     2101 gaggtaataa tatgattaat acattagatt taataggaaa cactcctgtg attaaattac
     2161 cgggagaaaa tatttattta aaaatggaaa aattcaatcc cggcggcagt gttaaggaca
     2221 gagccgcatt agggatgatt gaagatgctg aagcaaaggg atatttaaat aaaaacagca
     2281 tcattgtgga gcctaccagc ggaaatacag gtattgcact ggctctaata ggaagattga
     2341 aaggatataa agtaataatc gttatgcctg aaactatgag tatggaaaga agaaacataa
     2401 tcgaagcaca tggtgccaca ttgatactta cggaaggaca aaaaggaatg agcggagcaa
     2461 tagcaagggc tcatgagctg cttaaagatg ataataatta ttttatgcct gaccagttta
     2521 ataatcctgc aaaccccagg attcactatg atactacagg ccaggaaatc attaggcagc
     2581 taaacgatat tgatatattc acagccggag tcggtaccgg aggaactcta accggagttt
     2641 cgaaaaggct gaaagaatat aacaaaaaca taattactgc aggagttgag ccttcaaagt
     2701 cacctgtatt atcaggcggc agcccgtcac ctcataaaat tcagggaata ggggcaggct
     2761 ttgtaccggg aacatacaat ggaagttttg tagatgaaat aataacaata accgatgagg
     2821 aagcaatcga tactgctgta gaagtttcag caaaaattgg aatgctcata ggaatttcgt
     2881 caggagctaa tgttgcggca gcaagaaaat tagcgcaaaa atacggcagc aataaaaaaa
     2941 tacttacaat atctccggac ggcggagaaa agtatttgtc ggtagttaag tattaattga
     3001 aaggaatgat atcatgatta aatttataaa atcaataatt gaagatatag attctgtgtt
     3061 tgacagggac cctgccgcaa gaagcaggat ggaagtctta cttttgtatc ctcatattaa
     3121 agccttgata gcatataaaa tttctcataa attatattta aaaagaagat attttttggc
     3181 aagacttata tcaaatattg caaggcacag gacaggaatt gaaatacatc ccggcgcaac
     3241 catcggtaaa ggacttttta tagaccacgg tatgggtgtt gtaataggag aaacagccgt
     3301 tgtagggaac aatgtgacaa tgttccatgg agctacattg ggaggtaccg gaaatgaaaa
     3361 agggaaaaga catcctactg tcggggataa tgtagtaata ggagcatcgg caaagatact
     3421 tggaaacatc tatattgctt caggaaccaa ggtaggagca aatgccgtcg tattgaatga
     3481 taccaaccct aattctactc ttgtcgggat tcctgcaaga gaggttaaaa aagggaacaa
     3541 aataattaac ttgagcgaat atgcaatata atgcatgggg acacaccttc aatatacagt
     3601 gattctttgt gagataatta ttgaaggaat gtccccgtaa tgagggggat atgcgtgtcc
     3661 ccaatggttt acataatgtt caattatgta aaccatattt tattctctcg tgttaacatt
     3721 atcgtagtct accaataagg gctcaccttc ttcgattctt atatgtcctg attcgtaagg
     3781 acctccgtca attgttaaat atacttcctg aactccgtag tagttgccta aggtgttggt
     3841 taaactttga agtatcaggt tttcaaaact tgaccctgca ttcatttctg ttatgaaatc
     3901 atcggacaaa tcaatatgaa cgctgttttc ttcctcatca aagtataatt cgttgattat
     3961 cgtattgggg cttaaaatct cataaacaga taactccttg acaacttctt ctataatatc
     4021 ttttggctcg tcattggtgt tgaattctat ttgaacatct attgtatcaa tggagccgtc
     4081 cgtattggga taaaacaaag taatagtctg tatcaggggt atattattct ctatagatga
     4141 taaagttgaa gatacctcat aaccttcacc agagcttatt gttttaatca atcccacatc
     4201 ctttgcataa tagtttactg tttttccttg ctctccttcc aaggtaactt caattgcctg
     4261 ataattgcca agtgtagtga ctatttcttt tgataagtcg gttatggttg cagtcgtgtt
     4321 ttcatcatat gcccacgaat tcccttcctc taagggacct ttcagaagaa cttttccctc
     4381 accatattct ttgtttaaaa agttttgtct aaaatatgtt tctcctcttg aatataataa
     4441 agtcaattga tcatccttta tttcatacac attaaccgtt tctgttccac cgttgtttgt
     4501 tctattttga atgcggttgt ctgaaacata atcaataaat gctacataag ttgcaaattc
     4561 atttccttca ccttcatatg aatactttgt attttcttta ataggataat attcttctat
     4621 tgatactgtc tcgccattat caggttcttc aggcgggaca gcaatgttgt tacatgctgt
     4681 aagaataaca gcaagcacta tcgaaatcac taataacttt ttcattaaat tctcctcttt
     4741 acataatttt gttaatttat tatacccaga tttatcacta ataattaccg ttataaaaaa
     4801 ataaccgggt ttcggttatt ataaatcctt cgattaggct cgggatgaca atcacagaga
     4861 agagtcttat gaaatccttc gcttaggctc aggatgacaa agtccaaagc tctcccaatg
     4921 ttttaaggaa tcaaccatat actctgttac attcatatca tagacttcat ttaaaagatt
     4981 agaagaaata atttcatttg tactgccgta agcagcaata tttccattat ctaataccag
     5041 catattatca gacaatttta ttgccaaatt taaatcatgc attacaccga ttacagaatg
     5101 gccttcatat tttgaccaat ccttcaaaaa gtcgattaac tctgtctgat gttttaaatc
     5161 caaatgattt gttggctcat caagaagtat aatgtttggc tcttgagcta aggttcttgc
     5221 caaataaacc ctttgaagct gccctcccga caaggtgctt atttgcttat gcttaatatt
     5281 aaataaacct acagctttta aacatttatc cgtatattct ctatcctttg cggaaggctc
     5341 tttaaatgtt cttcccttca tatgaagata tcttccgaga agtacggttt cgtaaatgct
     5401 gtatgaaaag taaatggttg aaatttggga cataacagct attttctttg caacttgctc
     5461 ccttttcata ttattaatat cagttccgcc tatcttaata attcccgtat gaggaataat
     5521 tcctgaaatt gccttaataa gcgtagtctt tccgcaaccg ttgggaccga taatgcaaag
     5581 attttcacct tccttaacgc ataaggaaat attatttatt acatcaattc cgttataacc
     5641 tgcagataca ttttttaaag taagcatagc atctataacc cctttcttct cgaaaagtat
     5701 acataagcaa aaaacggtgc acctattaat gccgtaattg ctcccaccgg caattcagaa
     5761 ggcgatataa tcgttctggc aatcaagtcg catagaacca tgaatgagcc tccgaaaaac
     5821 actgacatcg gtattactgt tttgtgattg gagccgaata tttttcttac cacatgaggt
     5881 gcaattaaat caataaatcc gattactccc acaaatgcaa tggcactgcc tgtcagtgct
     5941 gccgaaatgc tgagaagaat ccatttgact ttgtggacat ccactcccat ggtgaatgcc
     6001 tgctcctctc caaaagtcat tatatccatt tcttttgaat aattttgcaa tactgtaatt
     6061 cctattaaaa cgatgggaaa gagtattatt acactagacc agtcttttaa tgaaaaactt
     6121 cccatttgcc aataagtcag ctgtgcaaga tggtctcttg ccattgcagt tatgacagtc
     6181 agtatggcat ttacaaacaa tgaaaaaacc atacctacca aaattattgt attagtttct
     6241 aaatttttat ccatcatagc tgcgaattta acagccaaaa ataccgtcgc catgccaaat
     6301 accaagccgg taacaggcaa tgtgaacatt cccaggaagg gtatgttgaa cccggttact
     6361 attactagag ctgctcccaa tgaagcacct gaagataccc ctaatgtgta tgaggaagca
     6421 agaggatttt tcaaaaccga ctgcattacc gagccgctta cagctacagc accccccacc
     6481 aaaaatgcca agagtgttct tggaagtctt aacttccata ttattgatat agcagtggta
     6541 ccttctatat cggaaggaag cggtgctgaa aatattttgt ttaatattac acttataata
     6601 tgggaagggg gaacgttgac gcttcctaat cccactccca ttattactac tataatactg
     6661 aatattattg ataatattat tttaaatttt attttcattg atatatgtcc ggatagattg
     6721 ccctcgccat ttgctccaag cctgtaataa tgttgtggtt gcagtgggag gttgcataat
     6781 tgtctacata ataaacatca ttatttttaa cagccttcat attttcccat cctgagcggg
     6841 atttgatttc ttctgcggga ttttcaatat aatcaacatt ggttatgata acatcagggt
     6901 cagatgcaat aactgtttct tctgaaactg aaatccacca gtcatggtcc gataaaacat
     6961 tttcggcacc tattatttca atcatttcat ttaaaaatac tcccttgcca aaactgtaaa
     7021 gtgttggtgc tgctgcaatt tcaaaataca ctgtcttttt gttttcaata gatttgccga
     7081 tttcttcaat ttctttaatt ttagatttca tgctgtttac aaggtcaata cctttttttt
     7141 caacaccgag ggattttgca aaaaataata tatcttcata aattccctct atgcttaaac
     7201 tggccggtat atatgctaca caaacaccca tatccctgat aggtttgtaa gggtcgtttc
     7261 cttctgccat aaccattccg gtcgtataaa ttatgtccgg ctctaaggca accatctgct
     7321 cagcatcagg tgccatcata tcaaataatt gtatattatt aggcagtcct tcaatatctt
     7381 tagaatactc atcgactgca attattttat cgccaaaacc taattcaatt agtatttccg
     7441 tatttgcagg tgatatggaa attattctgt tgatttcgtt tggtattgat atttcattgc
     7501 ccgctctgtc ttttgtgggc aatgttatgt tgatatcctc cggttgagga gattttactt
     7561 cggtattctc ggcacaagca gtaagcaagg ccataataca aattaatgtt aaagctaaaa
     7621 tactgtttct tttcatttca tcctccattt ttgtttttta aacatctaag agaggcattg
     7681 cgtttgacct accatcaaga ctttctcctt ccggtcgaag tcttggctta gggcataaaa
     7741 aaacgtctcg ttcccgaaac gtaaattcca caaatatttt ggataaacca tttttgcgct
     7801 atcgctttta tactctgtaa agcatagcat ttgcggcatc tacgcaaata atactggcag
     7861 gttttccgac tcagaggtca tcgctggttt ttaaccttcc cggagatatc cagtggttgc
     7921 ataagcataa aaaccgctct ctcatcacgg cagcaggact gtttaggatt ttaacctaat
     7981 tccctattat cgcatttaag cgcaccaata tcgaaagatg tttaattaag ataagtatat
     8041 tacattaaat tagtattgtc aattaagaaa atatgcaaaa ttatatcgat acttatacta
     8101 aaactactta gcttttaaat tctatttacg ccggttttta ttgcagcttc agaaacagct
     8161 ctagctactg ctttggctac tcttttatcg aaagcttttg gaattatata ttcttcattt
     8221 aattcctctt cggttaccaa ttctgcaatt gcatgtgctg ctgctaattt catttcttca
     8281 ttaatttctt tagcccttac atcaagagca cctctgaaga taccggggaa tgctaataca
     8341 ttattaattt gattggggaa atcagatctt cctgttccca ttactttgac tttgcctgcc
     8401 aatgcgactt caggcaatat ttcaggtatc ggatttgcca tcgcaaaagc aattgaattt
     8461 tcattcatgc tttcaatcat ttcttttgta actatatttg gtgcagaaac acctataaat
     8521 atatccgctc cttttaaagc atccgaaaga gctccttttt tgtttgcctt atttgtcttt
     8581 aaggccatct cttttctaat cggatcaagc ctttcatctt ctttatctaa tatacctctg
     8641 ctgttacata taattacatc attgattcct gaactcagaa gcaatttaga tatagctata
     8701 ccggctgcac cggcaccact tacaacagct gttacatttt cgattttttt acctactgct
     8761 tttaacgcat tgattattcc tgctaataca acgacggcag taccatgttg atcatcatga
     8821 aatacgggaa tatccaattc tttctttagt ctttcttcta tttcaaagca ccttggtgca
     8881 gcaatatctt ccaaatttat accgccaaag gacggcgcaa tttgtttaac agtttgaact
     8941 actatatcaa catcttgact ttctatgcaa ataggaaaag catctacatt ggcaaatgat
     9001 ttaaataata ctgccttacc ttccataaca ggcattccgg ctcttgaacc tatattaccc
     9061 aaacctaata ctgcagagcc gtcagtaaca accgcaacca tattaccctt attagtatat
     9121 tcatatatta gttcaggatt cgcatggatt tccttgcacg gctcagctac tccgggtgta
     9181 taggctaagc ttaaatcctt tgcattttct actctgacct tacttataac ttcaatttta
     9241 cctttgttat ctttgtgtag ttttaaagct tcttctctta aatccatatt tactttctcc
     9301 ttcgtcctta attattaatt attataccat aactttacgt cttatacatt atttgaattt
     9361 tatattcatt taaaaaacaa aattaagaaa aagttgttga caaaaacatt aaattttaat
     9421 aatat
//
LOCUS       DAOSHP010000068         9338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_6285_length_9338_cov_9.813638, whole genome shotgun sequence.
ACCESSION   DAOSHP010000068 DAOSHP010000000
VERSION     DAOSHP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_9338_cov_9.813638"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLB33_07090"
     CDS             <1..474
                     /locus_tag="PLB33_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG63327.1"
                     /translation="ENPAMLFGIGLSSNVLGVLCGGFIGVFVTSIGLPDAEVAVIALT
                     MVCITLVMLPPLNNQLVLLLKNHAYLAVYDNMSRSQQTDIIRYAKTLDPLTVREEEVL
                     QLILTGKSNREIAELLFISENTVKTHARNIFSKYDVGSRAELISTLLKNQVSSIL"
     gene            683..2665
                     /locus_tag="PLB33_07095"
     CDS             683..2665
                     /locus_tag="PLB33_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63328.1"
                     /translation="MKNKIISLLLISSIIFNMVGFSGCSKAEEKLVDTSVQLPEHITG
                     TVSDENLTVSQDGVSFTIEEGSVLMDTDVEITLKKDLPPIIVGDEEIMLTAYDFSVAG
                     EEPLGVMTLEIPFTASDDEVFAAYYNLEKGEWEPVEFIYDAERKCAVIYTTHLSSYGL
                     FTVENENTRNALIAYTYNLELAEVNINEAIRMLENAMEDPDTFYEDCFDLISNSVSIH
                     SSITTNLVDVVSGLSSYVPTFSEGLKDNVTKFGYLLTGIEVVNAFMNDDKPAAVWKMF
                     DTAVGFNVDKIASLSGTASMAAGLFSVGLVSYALNKFISTALQGRTDMYERAYNLYYK
                     ENGRTTAEWVYLIRAMVENGKDPEKTGEAISNEIKRYVNEFWEDDLTIAEYGKDANPN
                     AWSSGSLGGLNDGIKKEISNYYQMTIERVVGNILNRIQIENKINMQSEMRKELFNFQK
                     KMNSIVTISVKDSKAENEPSHFAGAVIRFSELPSSIEDPEKWESKISEKGAARIQFRV
                     LAHLLAGSPRKMEIVKKSGDKEDIMVRFNAKFSLPESKLDLADLYPRSDYPKNIGGSY
                     TVSMYNTTTGKTIDDLMLKVTVTQEVSDGCYADFYLENSGSVYLNGNYFYRYATGKVN
                     YALGDMSFSGDDNNVSFKFDAYDNQKKVWGAFTGHK"
     gene            2710..3219
                     /locus_tag="PLB33_07100"
     CDS             2710..3219
                     /locus_tag="PLB33_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63329.1"
                     /translation="MKSINKMRNYYLVFFVIAALLTLWFALKFIYEAAFIFGNISIVL
                     LLYVIKQNRLLYDTRLIYDNCIFKIPSATVSTSNSEIKLAVEEIVVSAFGLIIGNKIY
                     KWGYHGAYGVKLKAIEINPERVYLTFGDASKTARVELLHGITNKQEVLDIKQKFWRET
                     GIDTVVNGW"
     gene            3312..3497
                     /locus_tag="PLB33_07105"
     CDS             3312..3497
                     /locus_tag="PLB33_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOG63330.1"
                     /translation="MKKITAIIGSARKGATYQAVREFEQNLKEYEEIDFEYIFLSDYN
                     LEFCRGCKICFDKGEAY"
     gene            3629..4711
                     /gene="rsgA"
                     /locus_tag="PLB33_07110"
     CDS             3629..4711
                     /gene="rsgA"
                     /locus_tag="PLB33_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOG63331.1"
                     /translation="MSKINMENIGLSQRFKNESTLYEGLYIGRIASQYKGLYKVITED
                     GILTAEVSGKFRFDAKTTSDYPAVGDFVMIDRNDDKDGNAIIHHVLTRKSAFVRKAAG
                     TSNEEQIVASNIDTVFICMSLNNDFNIRRIERYLGIAWDSGASPVIVLTKSDLCDNIS
                     EKLAELDTVAIGADVIVTSSMSEDGYLPIKRYLGSGKTIAFIGSSGVGKSTLINKLIG
                     EDVLETNGLRNDDKGRHTTTRREMLLIPDGGVVIDTPGMREIGIESADLTKTFADIDE
                     LSAKCKFSDCTHINEPKCAVQEAISNGLLSRDRFESYLKLKKEAKYDGLNSRQIENKK
                     LNEMFKDFGGMKNVRKMLKEKKKLER"
     gene            4800..5666
                     /locus_tag="PLB33_07115"
     CDS             4800..5666
                     /locus_tag="PLB33_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOG63332.1"
                     /translation="MLNELNRVIDYIEEHLTDDELSLEIISEYAGVSDYHFRKIFFYL
                     SGMTLSEYIKNRKLSEASMDLLHGEKVTDVAFKYGYQSMDGFTRAFKKWCGLLPSEVI
                     KNGISKSFPKLSFIITVKGGINMEFRIENKPAFNLVGVCKRVPMQFEGINNEIVKLAE
                     SITDEQREEMHSLQNIEPYEIVNASYEADADFLKEEGYLTHLIGVLTTKNEISDLLEK
                     VPVEAHTWAVFPNEGPFPSTLQETYAKIYSEWLPSSGYEVINAPTFSFTKMDKNKENY
                     AYSEVWTPIRKK"
     gene            complement(5717..6154)
                     /locus_tag="PLB33_07120"
     CDS             complement(5717..6154)
                     /locus_tag="PLB33_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63333.1"
                     /translation="MNNNQNTVEILSEIYRGAKMGVETINSMLNKVNDNKIYDELKYQ
                     LRSYDEIANEAYGELVKRNQEPKDISTVNKLSAKMSVGINTMISNTPSHIADMLIKGS
                     TMGVTEMTKSLNSYKDADPDIQALADRFIKLEQDNIDRLMKFL"
     gene            6382..6633
                     /locus_tag="PLB33_07125"
     CDS             6382..6633
                     /locus_tag="PLB33_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63334.1"
                     /translation="MSDKSKKSKDKYNIQWDINSMNASSSTELTGLIPVGPDSDEELA
                     SYDEILTYRAQNTVAESNEKSMNQTLHARKTPDFKNKKS"
     gene            complement(6681..7229)
                     /locus_tag="PLB33_07130"
     CDS             complement(6681..7229)
                     /locus_tag="PLB33_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63335.1"
                     /translation="MGLSIQYEIVSEDNIFIIKDLCNELMAYQKSKAYIHPEFFDNMS
                     FETRVLPAMKSSKENYIIAANDGDEVVGYAYSTIAPKTIYSGGFATLQCDAFFDFDSV
                     ETEDVGCLSQFFIKENYRNTGIGTAMFNVSMDWLNSFKEIKDIFIFVSNGNENALNFY
                     LNKGFKVSHEILDGFITVLRNK"
     gene            7359..7700
                     /locus_tag="PLB33_07135"
     CDS             7359..7700
                     /locus_tag="PLB33_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG63336.1"
                     /translation="MNDNAPYKNNYDSCPLTFALTLIGGKWRLPVIWSIHKNKTMRYN
                     ELRRDIQGITSMMLTQSLKELEAYGIVERKQYAEIPPRVEYSLTDNGENLIPALKALA
                     DWGDKMRKLKA"
     gene            7710..8279
                     /locus_tag="PLB33_07140"
     CDS             7710..8279
                     /locus_tag="PLB33_07140"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOG63337.1"
                     /translation="MYTIDELKKFCYRCHKCRLAETRNNVVFGEGNENADIMFVGEGP
                     GYYEDIQGRPFVGRAGQLLDKMIEAIGFKRNDVYIANVVKCRPPENRNPFDDECSACL
                     DYLRWQVKLIKPKIIVCLGAVSAKNLISPDLSISRQRGEFVIKGGIYFMPTYHPSYLL
                     RNESAKKDSWNDFKNIAAKIEEIRGTALD"
     gene            complement(8317..8949)
                     /locus_tag="PLB33_07145"
     CDS             complement(8317..8949)
                     /locus_tag="PLB33_07145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63338.1"
                     /translation="MEVMSTLQDYDVSQFIQIPDLVKSRKATPKDLSDIYKVACSVGS
                     KSKESYAGFLMDDYKSRPKYYQKFFLERIFELDYFYVAESNIGIVGFLMAYTKEQWLK
                     YNPTWIEDIMWRPDFSMEKTSDFVIVDKTAIMANLTGKGIGSTLYKALLKDLKKKNIK
                     HIFAETLVDPVPNFASLAFRQKQKYTLAGTRYENYMDTLYTDLIYYKPVN"
     regulatory      complement(9019..9150)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT         3161 a         1425 c         1855 g         2897 t
ORIGIN      
        1 gagaatccgg caatgctgtt tggcatcgga ctatcttcaa atgtgttagg tgttctttgc
       61 ggcggtttca taggagtgtt tgtgacatcc attggacttc ctgatgcgga ggtggcggta
      121 attgctctta ccatggtctg cattactctt gttatgctgc cgccacttaa caatcagctt
      181 gtactgctgc tgaaaaatca tgcttatctt gcggtttatg ataatatgag ccggtcacag
      241 caaacggaca tcatccgata tgctaaaaca ctggatccgt tgacagttcg ggaagaggag
      301 gtgttgcagc ttattttaac aggtaagtct aacagagaaa ttgccgagct tttattcatt
      361 agcgagaaca ccgtaaagac gcatgcaagg aacatttttt caaaatatga tgtaggcagt
      421 agggcagagt taataagcac cctcctcaaa aatcaagtca gttctatact ataagtttat
      481 ataaatattg ataaatagaa cccctcatac taaagtatga ggtttttttt gtaaattcat
      541 cctttcgaac gatgctttat taaggtttct ctcttaaaat atccaatata agaggatgta
      601 tcaaaactct catatacaaa aatctcataa cattataaag gaggaaaaac tatatgccaa
      661 ggataacaaa aaggaggaca aaatgaaaaa taaaataata tctctactat taattagttc
      721 aataatattt aatatggtcg gcttttccgg ttgcagtaaa gccgaagaaa agcttgttga
      781 tacatctgtt cagctgcctg aacatataac aggtaccgta tcagatgaaa acttgactgt
      841 ttctcaggat ggagtttcat ttaccataga ggaaggatca gtgctaatgg atactgatgt
      901 agaaataact ttaaaaaagg atttgcctcc gattatagtc ggagacgagg aaattatgct
      961 cactgcttat gatttcagcg ttgccggtga ggaaccctta ggtgtcatga cgttggaaat
     1021 tccttttact gcttcagatg atgaggtttt tgccgcctat tacaatttgg aaaagggaga
     1081 atgggagccg gtagagttta tatatgatgc ggaaagaaag tgcgctgtta tatataccac
     1141 tcatttatct tcctatggtc tatttactgt tgaaaacgag aatacacgaa atgcgctgat
     1201 tgcttacacc tacaatttgg aattggcgga agtcaatata aacgaggcaa taagaatgct
     1261 tgaaaatgct atggaagacc ctgatacctt ctatgaagat tgttttgatt taatttcaaa
     1321 cagtgtttct attcatagca gtattacaac taatctggtg gatgttgtgt ccggattgtc
     1381 ttcatatgtt cccactttta gtgaaggatt gaaggataat gttacaaaat ttggatattt
     1441 attgaccggt attgaggttg tgaatgcatt tatgaatgat gataaaccgg ccgcagtatg
     1501 gaagatgttt gatacagcag taggattcaa cgtggataaa atcgcatcct tgagcggtac
     1561 tgcctctatg gcggcaggtt tattctcggt cggacttgta agctatgcat taaataaatt
     1621 tatttcaacc gcattacaag gaagaaccga tatgtatgaa agggcgtata atctttatta
     1681 taaggaaaat ggcagaacaa ctgcagaatg ggtttattta attagagcaa tggtagagaa
     1741 cggcaaggac cccgagaaaa ccggcgaggc aatttctaat gagattaagc gatatgttaa
     1801 tgaattttgg gaggatgatc ttactattgc cgaatatggc aaagatgcca accctaatgc
     1861 atggtcatca ggctcattag gaggacttaa cgatggtatt aagaaagaaa taagtaacta
     1921 ctatcaaatg acaattgaaa gagttgtagg gaatatacta aatcgtattc aaatagaaaa
     1981 caaaatcaac atgcaatcag aaatgcgtaa agaattattt aattttcaga aaaaaatgaa
     2041 ttccatagtt actatatcag taaaagacag caaggcagaa aatgagccgt cgcactttgc
     2101 aggggctgtc ataagatttt ctgagcttcc ttcaagtatt gaagacccgg agaaatggga
     2161 aagtaaaatt tcagaaaagg gtgcagcaag aatccaattt cgagtattgg ctcatctttt
     2221 agccggcagt ccgagaaaga tggaaatagt gaagaagtcg ggtgacaaag aagatattat
     2281 ggtgagattc aatgcaaagt tcagtttgcc tgaatccaaa cttgatttag ctgaccttta
     2341 tccgagatca gattatccta aaaatatagg ggggagctat actgtttcaa tgtacaatac
     2401 aactacagga aaaacaattg atgatttgat gctgaaggtt accgttacac aagaggtttc
     2461 cgacggttgt tatgctgatt tctatcttga aaactcaggc tcggtatatt tgaatggaaa
     2521 ctacttttat cgctatgcaa caggtaaggt caattatgcc cttggagata tgagtttctc
     2581 aggtgacgat aacaatgtaa gctttaaatt tgatgcatat gacaatcaaa aaaaggtatg
     2641 gggggccttt accggacata aataacactc tttacagggc agaatagaca taaaaggggt
     2701 gtgatacaag tgaagtctat taataaaatg cgtaattatt atcttgtatt ttttgtaatt
     2761 gctgctttgt tgactctatg gtttgctttg aagtttattt atgaggcagc gtttatattt
     2821 gggaatataa gtattgtgct tcttctttat gtaattaaac aaaaccgact gctgtacgat
     2881 accaggctca tttatgataa ctgcattttt aaaataccat cagcaaccgt ctccacatca
     2941 aacagtgaaa taaaattagc ggtcgaagaa attgtggtgt ctgcttttgg acttattata
     3001 ggcaacaaaa tttacaaatg gggttatcac ggagcatatg gcgtaaaatt gaaagccatt
     3061 gagattaatc cggagcgagt ttatttaacc tttggtgatg catcaaaaac tgcgagggtc
     3121 gaattattgc acggcatcac aaataagcag gaagtgctgg atataaagca gaagttttgg
     3181 agagaaacag gtatagatac agttgttaac ggttggtaaa aaaacttctt gattatggaa
     3241 gaatatgtta taatgcatat aggtataaga aagtgtttta atctaaagta tttgttgtca
     3301 gggggaatat tgtgaaaaaa ataacagcaa ttataggcag cgcaagaaaa ggagcaactt
     3361 atcaggcagt gagggaattt gaacagaatt taaaagagta tgaagaaatt gattttgaat
     3421 acattttctt aagtgactac aatcttgaat tttgccgagg atgtaagatt tgttttgaca
     3481 agggagaagc gtattgatga aaggagttgg tattcatgtt taataagata gtaacggata
     3541 ttgcagtaaa tggggaggtg catacaaaat aataaagtat aaacctccaa aaagtcataa
     3601 cataatatga atattaggag gaataaaatt gagtaaaata aatatggaga atataggact
     3661 cagccaaagg ttcaaaaatg agtctacttt atatgaagga ctttatattg ggagaatagc
     3721 ttctcagtac aaaggtttat acaaggttat aacagaagat ggcatattaa cagcagaggt
     3781 ttcagggaaa tttcgttttg atgcaaaaac aacatctgat tatccggctg taggggattt
     3841 cgttatgatt gacagaaatg acgataaaga tggaaatgca ataattcatc atgtccttac
     3901 aagaaaaagt gcctttgtca gaaaagctgc aggaacatca aatgaagagc aaatagttgc
     3961 ttccaatatt gatactgttt ttatttgtat gtcgcttaat aatgatttca atattcgtag
     4021 aattgagcgc tacttaggga ttgcatggga cagcggagca agtccggtta tagtgcttac
     4081 caaatccgat ttatgcgata atatctctga aaaattggca gagcttgata ctgttgctat
     4141 cggagcagat gtaatagtta cttcaagcat gagcgaggat ggttatttac cgattaaaag
     4201 atatcttggc agcggaaaaa ccattgcctt tataggttct tcaggtgtcg gtaagtcaac
     4261 cttgattaac aaacttatcg gtgaagatgt ccttgaaact aacgggctaa gaaatgatga
     4321 taaaggcaga catacaacga ccagaagaga gatgcttctt atacctgacg gcggtgttgt
     4381 tattgatact ccgggaatga gggaaatagg tattgaaagt gctgatttaa caaagacttt
     4441 tgccgatatt gatgagttgt ccgctaagtg caagtttagc gattgtacgc atattaatga
     4501 gccaaaatgt gctgtgcaag aagccataag taacggctta ctgtcaagag atagatttga
     4561 aagttattta aaactaaaaa aagaagctaa atacgatgga ttaaattcca ggcaaatcga
     4621 gaataaaaag cttaatgaaa tgttcaagga ttttggcggc atgaagaatg tccgaaagat
     4681 gttgaaagag aagaaaaaat tagaacgttg atatcctttg attcgatatt ttttataata
     4741 aaaaaatcga gcattataaa aatatctttg gtattatgaa gacgaaggat ggggaatgaa
     4801 tgttaaacga attaaaccgt gtaattgatt atattgaaga acatttaacc gatgatgaac
     4861 tatctcttga aataatttct gaatatgccg gggtttccga ctatcacttt agaaagatat
     4921 tcttttatct ttcaggaatg acccttagcg agtatatcaa aaacagaaaa ttgtcggaag
     4981 cgagcatgga tttactgcat ggagaaaaag taacggatgt tgcttttaaa tacggttatc
     5041 agtcaatgga cggttttact cgagcattca aaaagtggtg cggattattg ccttcagaag
     5101 taataaaaaa tggaataagc aagtcctttc ccaaactttc atttataata acagtcaaag
     5161 gaggaattaa tatggaattt agaattgaaa ataaaccggc gtttaattta gtcggagttt
     5221 gcaagcgtgt ccccatgcaa tttgaaggaa taaataacga gattgtaaag cttgcggaaa
     5281 gtataactga cgagcaaaga gaagaaatgc attctcttca gaatattgaa ccttatgaaa
     5341 tagtcaatgc ttcttatgaa gcagatgctg atttcttaaa agaagaagga tatttaaccc
     5401 acttgatagg tgtgttaaca acaaaaaatg aaattagtga tttattggaa aaggttcctg
     5461 ttgaagctca cacatgggcg gtgtttccga atgaaggacc atttccgtct acgttacaag
     5521 aaacatatgc aaaaatatat tcggaatggc ttccatcttc cgggtatgaa gtaataaacg
     5581 ctcctacttt ttcttttacc aaaatggata aaaataaaga aaattacgcc tacagcgaag
     5641 tttggactcc cataagaaag aaataagcat ttaaaacccc cactatttaa aagtgggggt
     5701 tttttgtctg ggtttattat aagaatttca ttaacctgtc aatattatct tgttccaatt
     5761 ttataaacct gtctgccagc gcctgaatat cagggtctgc atctttgtac gaatttaagc
     5821 ttttagtcat ttccgtaact cccatggtac tgcctttgat aagcatatcg gcaatatgag
     5881 atggtgtatt actaatcata gtattaatac ctacgctcat tttggcgcta agcttattca
     5941 cggtggaaat atctttgggt tcttgatttc tttttaccag ttcgccatac gcttcattag
     6001 caatttcatc ataacttcta agttgatact taagttcatc gtatattttg ttgtcattaa
     6061 ctttatttag catactgtta attgtttcaa cgcccatttt agctccgcga taaatttctg
     6121 ataaaatttc tacagtattt tgattattat tcatttgttt acctcctttt tagtttatta
     6181 tttacaatat taaaattctg atacaaaaac ttagtaaatg ataatagatt gatgatctaa
     6241 ggcaataata taaaataaaa ctttgagcat ttaatgcagt tataaaatac ttacttagca
     6301 ttctcatttt aagggggaaa ttttgtttaa aatttctctt ttcatgacat actataaata
     6361 aaatacagaa ggagattcag catgagcgac aaaagtaaaa aatcaaaaga taaatataat
     6421 attcagtggg atattaattc tatgaatgcc agttcatcta cagagttaac gggattaatt
     6481 ccggtaggac ctgattcgga cgaagaatta gcatcttatg atgaaattct gacatataga
     6541 gctcaaaaca cagttgcaga gagcaatgaa aaaagtatga accagacact gcatgcaaga
     6601 aaaacacctg atttcaaaaa caaaaagagc taataaatga ttcccttgaa taataaaatt
     6661 caagggaatt tttgtgtata ttatttattt ctcaatacgg ttataaaccc gtcaagaatt
     6721 tcatggctta ccttgaaacc tttattcaga taaaaattaa gtgcattttc atttccgttt
     6781 gatacaaaga taaatatatc ttttatttct ttaaatgaat tcagccaatc catggacacg
     6841 ttgaacattg ccgtaccgat accggtattg cgataatttt cctttataaa aaattgagag
     6901 aggcatccta catcttccgt ttccacagaa tcaaaatcaa aaaaggcatc acattgtaaa
     6961 gtagcaaagc cgccggagta tattgtcttc ggtgcaattg tgctgtaagc atatccgaca
     7021 acttcatcgc cgtcattagc tgctattatg taattttctt ttgagctttt catggccggt
     7081 agcacccttg tttcaaaact catattgtca aaaaattcag gatggatata tgccttggat
     7141 ttttgataag ccattagctc attgcataaa tctttaataa taaaaatatt atcttctgaa
     7201 acaatttcat attgtatact caaacccatc atcctttcgt aataaaaatt tgactgatta
     7261 attaatcttc gattataatt atatcagcta aaaaaaatgt aacaagtatg caatatttta
     7321 tgtctaagta agaaaaaact gagtaatgga gtggttaaat gaatgacaat gcaccttata
     7381 agaataatta tgacagctgt ccgcttacat ttgccttaac tctcataggc ggaaagtgga
     7441 gattgcctgt tatttggtct attcataaaa ataaaactat gcgttataac gagttaagaa
     7501 gagatattca gggtattaca agcatgatgt taacccagtc gctaaaggaa ttggaagctt
     7561 acggtatcgt cgagagaaag cagtatgctg agataccgcc cagggtagaa tattccttaa
     7621 ctgataacgg agaaaatctt atcccggctc ttaaggcctt ggctgattgg ggagataaaa
     7681 tgaggaagct gaaagcctga ggaggtatta tgtatacgat tgatgaacta aaaaagtttt
     7741 gctatagatg tcataagtgc agattagctg aaacaagaaa taatgttgtt tttggagaag
     7801 gaaatgagaa tgctgacata atgtttgtag gggaaggtcc gggatattat gaggatattc
     7861 aagggaggcc ttttgtcggc agagcaggac agcttcttga taaaatgatc gaagcaatag
     7921 gctttaaacg aaatgatgta tatatagcca atgttgtaaa atgcaggcct cctgaaaaca
     7981 gaaatccttt tgatgatgaa tgcagtgcat gcctggatta tttaagatgg caggttaagt
     8041 tgattaaacc aaaaattatt gtatgtcttg gagcagtatc cgctaaaaat ctcataagcc
     8101 cggatttaag tatcagcagg caaaggggag agtttgtaat taagggaggc atttatttta
     8161 tgcctacata tcatccgtca tatctgttaa gaaacgaatc agctaagaag gattcatgga
     8221 atgattttaa aaatattgct gctaaaatcg aggaaatacg tggaacagcc cttgattaag
     8281 caaataaaaa atgactcttg atagagtcat taaggattag tttactggtt tataataaat
     8341 caagtcagta tatagagtat ccatataatt ttcataacgt gtgccggcta aagtatattt
     8401 ctgcttttgt ctaaaagcca gagaagcaaa attaggtacc gggtctacaa gcgtctctgc
     8461 aaatatatgt tttatatttt tctttttcaa atcttttaag agagctttat aaagtgtgct
     8521 tcctatacct ttaccggtta gatttgccat tatagctgtc ttgtcaacta tcacaaagtc
     8581 agaagttttt tccatcgaga agtccggtcg ccacataatg tcttcaatcc aagtgggatt
     8641 gtattttaac cattgttctt tggtgtaggc cattaaaaaa cctactatcc cgatattgct
     8701 ttcagctaca taaaagtaat ccagttcaaa aattctttcc aagaagaatt tttgataata
     8761 ttttggtctc gatttatagt catccataag gaaacctgcg taagattctt ttgattttga
     8821 accaaccgag catgcaactt tatagatgtc tgataaatct ttcggtgttg cttttcttga
     8881 cttaactagg tccggaattt gaataaattg acttacatca taatcttgta aagtgctcat
     8941 aacttccata ttgtcatcct ccctttaaaa ttagcgattg caaaaatagg aaaatgatat
     9001 atgcagttat ttgtttcatt ttcctcttcc aacgcttacg aggttagctg acgggttcgg
     9061 gtcgaaagaa gttaacccta ctcatagaga ttcaccccaa aaaattggtt tccccgcttt
     9121 ttcatacaaa aaattcagcg ttacaattta caatattaca atctacttca tagaagtata
     9181 ccatattttt gatttattgt aaagaataaa ttttgaaaaa tcatgtagac ctgcaattct
     9241 aagtggtgta cgtgttgcat cttgtcgaac ttattcttta ttcttgtcct ttatattctg
     9301 ccatggtata atttaacagg aacatatttg attgtttg
//
LOCUS       DAOSHP010000069         9261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_6346_length_9261_cov_8.498262, whole genome shotgun sequence.
ACCESSION   DAOSHP010000069 DAOSHP010000000
VERSION     DAOSHP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6346_length_9261_cov_8.498262"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..2333)
                     /locus_tag="PLB33_07150"
     CDS             complement(237..2333)
                     /locus_tag="PLB33_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOG63339.1"
                     /translation="MRIKYIKWPILILLLILIALVQYSAPDAYAEDSIKIVIDGKRIK
                     PDVDPYISNDRTLVPIRVIAEELDSVVEWDNDNRAVHISKEDIHILLRIDSYLVEYTN
                     DNETSYTIIDVTPQITGDRTFVPIRLISNALGVKIEWDNDKRTVYIDSSESSEVTKFF
                     DVEISSVKPGQTITGTTELYTKINNEIPKGAKEIKYLLLDRDTAKGFVIAGGSNINTP
                     YEWVPAMEDNGQKVLVSAFYDAQGNFLAGDSIPVTVKINPQIELKGIREGQLITAHSV
                     PLSTKLNFSASYVKYEMINPDNGAVYLSPGVDPEQSFTMIPVMEDNGNMSVRVIAYDT
                     KGNAYKGEYVNIGIDVDRYLYLGGVKNGQTIDGSVTLLAQRNFNVTDTEYIMVDNSTG
                     EETLLFKSGYGSYSWFPGPEDAGSKDLYVRVKDTAGNTHVSSRVTVNVIGTPKLMLQG
                     VGPGQVLTQAADLNIKTNVKLDSIKYILTNVRTGNEIIISEKSTGVIIPEKGDDGTWT
                     IRAQGTYGGRTIKSEEVKFTIYTGQLYSAKPVIEKDKYLDLVSGLAVNTRKTTGMSAA
                     LQVAQAILETGWGQSVPVDKYDGKFSYNLFGIKGEGTKGSVTSNTWEEYNGVAFRIDA
                     EFRAYNNEKESWQDHKDLLLLRERYAPFREVMYDSTKGAWALKRCGYATDSQYAIKLI
                     DIIERYDLKELDEVTI"
     gene            complement(2449..2958)
                     /locus_tag="PLB33_07155"
     CDS             complement(2449..2958)
                     /locus_tag="PLB33_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63340.1"
                     /translation="MRVKGNVKNTAGYKYFICFITGLLWGTVIGSLGISILVSHRMEE
                     LYEKIAYLEHTIQDKDEKLEKLESSINNANIVLKDISVILEFTGLSKEQIDLIDNIEI
                     EKAIKEKYVSSLGKEIKSLDADILVQVIDKRILRFDDTEYQLTVNKLVLSDILKLWVG
                     VSLLEVTSP"
     gene            complement(2958..3296)
                     /locus_tag="PLB33_07160"
     CDS             complement(2958..3296)
                     /locus_tag="PLB33_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HOG63341.1"
                     /translation="MSSFYSSIVHNFLIAFGVVIGASLFSGLGALMTGTPPLKVMTDV
                     AGSIKLWAIAASLGGTFSSFLVIEKGLFEGEIKTLLKQVFYIVISLTGANMGYEFIRL
                     LQRCGNIWGK"
     gene            complement(3427..4518)
                     /locus_tag="PLB33_07165"
     CDS             complement(3427..4518)
                     /locus_tag="PLB33_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG63342.1"
                     /translation="MKKFLSVFLTLALLLTLFPLTALASEPFSLEAPKNLTGELKIRD
                     DGIPYFELKLTVPESVKTINSNLVDNPDYYDGTSCDGIEIIFDYKYGNYDWNEGPSLY
                     WNTSMAVSDYLELGAYEYEPFDEGDFDTVDIKNEVYQFRARFNALWGYQGDWIDKDIY
                     SEYSNIVTIGNPAFYDDASDWAVVELEKANEAGLIPDILKGKDLTKSITREEFAELAL
                     LLYEKTTDKTVASVSENPFTDTTNPQIVKAFSIGITQGTSATTFSPNELINREQCATM
                     LFRTIKAIEPEGDFSIEGVKDFPDQADISSWAVEGTKFMAKLGIIKGDSNGNFMPKAV
                     TDAQKAVGYGMASREAAILMSVRTYEEYK"
     gene            complement(4621..>5363)
                     /locus_tag="PLB33_07170"
     CDS             complement(4621..>5363)
                     /locus_tag="PLB33_07170"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOG63343.1"
                     /translation="SNLLYHGCIPVNEDGTFKKVTVGRTGKAYSGKEYFDRLEILVRE
                     GYFHKTNPDAKQYGMDITWYLWTGPDSPLFGKDRMTTFERYFIDDKKTHKETKSPYFK
                     LEDSEEMCRRIFEEFGLNPECSHIINGHVPVKSKSGESPIKANGKLIVIDGGFSRAYQ
                     STTGIAGYTLIYNSYGLLLVSHDPFESTQKAIEEEKDIHSTTMVLEKEVERKRVKDTD
                     AGEVMKAQIKDLEMLLDAYRLGLIKEQG"
     assembly_gap    5364..5463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5464..6770)
                     /locus_tag="PLB33_07175"
     CDS             complement(<5464..6770)
                     /locus_tag="PLB33_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HOG63344.1"
                     /translation="MTASNDDAIRMLDEEIRYLKLLSKQYPTIASACTEIINLQAILN
                     LPKGTEHFLSDIHGEYESFNHVLRNGSGNIKRKINEVYGDIMSEKEIKSLATLIYYPE
                     QKLEIIRKEEEDIEDWYRITIYRLVVICRKAAFKYTRMKVRKALPKEFAYIIEELMHK
                     GPEEDKEEYYSEIINTIIRIGRADEFIIAMAKLIQRLVIDRLHIVGDIFDRGPGADKV
                     MDTIMNYHTVDIQWGNHDVLWMGAAAGSEACIATVLRICARYANLDIIEDGYGINVLP
                     LATFALEYYGDDECIQFKPKVESDIVYSDKDLKLIAKMHKAIAIIQFKLEGHIIKRRP
                     YFKMDDRLLLEKINYEEGTIDLYGKTYHLNDRNFPTIDPKNPYALTKEEEELVEKIKS
                     SFLNSEKLQKHIRFMLSNGSMYLKFNSNLLYHGCIPVNEDGTF"
     assembly_gap    6809..6908
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6988..8064
                     /gene="buk"
                     /locus_tag="PLB33_07180"
     CDS             6988..8064
                     /gene="buk"
                     /locus_tag="PLB33_07180"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOG63345.1"
                     /translation="MKNSYKILAINPGSTSTKIAVYENEKLIFKETISHSNSDLEKFD
                     KITDQFKYRKDIILDTIKKHNFDLNELDAIVGRGGNFKPVEGGTYKVNKEMINDLIIG
                     VMGHHASNLGAIIADAIANDLGLSAYIVDPVVVDELEDYARISGMPEIERKAKAHPLN
                     QKAVARLAAAELGGKYSDYNFVVAHMGGGISIGAHKNGRIVDINNCLDGEGTFTPERA
                     GGLPVGSLVEMCFSGKYTIQEIKKKITGKGGLVAYLGTNDAMEVKKRIAAGDKYAKLI
                     YETMAYQIAKDIGAAATVLKGKIDNIILTGGLAHDDVLVQWIKERVEFIAPVKVYPGE
                     FEMHAMVQGVIRVLTGEEELKIYR"
     gene            complement(8091..8252)
                     /locus_tag="PLB33_07185"
     CDS             complement(8091..8252)
                     /locus_tag="PLB33_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63346.1"
                     /translation="MINLKRFLKYRDNAPEFKEIKDEGRVGLPCIVIENGEEILFDYN
                     LLDIESLKE"
     gene            complement(8508..8973)
                     /locus_tag="PLB33_07190"
                     /pseudo
     CDS             complement(8508..8973)
                     /locus_tag="PLB33_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
BASE COUNT         2688 a         1795 c         1416 g         3162 t
ORIGIN      
        1 cttatgtgta ttaccggttg ataaaattat tcttctcttc attcatcctt ccccgatttt
       61 tctttagtta gatccttcgc ttacgctcag gatgatatct tatatttatt atatcctaat
      121 tggatttatt taacaattat attgtaaccc gaaggaaggg gacaaacctt caaaatcagt
      181 tttaaataat aatttttgaa ggatgtcccc aactttttat tgctataata ataactctat
      241 attgttactt catccagctc ttttaaatca tatctctcaa taatgtctat cagtttgatt
      301 gcatactgtg aatctgttgc atatccgcat ctttttagcg cccaagctcc ttttgtgcta
      361 tcatacataa cttccctaaa aggtgcgtat ctttctctta gcagcaataa atccttgtgg
      421 tcctgccagc tttctttttc attgttgtat gctctgaatt cagcatctat tctgaaagca
      481 acgccgttat attcttccca ggtattggag gttacggagc cctttgtccc ttcaccttta
      541 attccaaaaa gattgtatga aaatttacca tcatatttat ccacgggtac actttgtccc
      601 cagcctgttt ccaatatagc ctgtgcaacc tgcaatgcgg cggacatgcc tgtggttttt
      661 cttgtattta cggctaaacc ggaaaccaag tccaaatatt tgtctttttc tattacaggt
      721 tttgctgaat ataactgacc tgtataaata gtaaatttaa cctcttcgga ttttattgtc
      781 ctaccgccgt atgtcccttg ggctcttatg gtccatgttc cgtcatcacc tttttcggga
      841 ataattactc cggtgctttt ctccgagatg ataatttcat taccagtcct aacatttgta
      901 agaatatatt taatactgtc taattttaca ttagttttta tatttaggtc tgcagcttga
      961 gttaatactt gtccgggtcc aacgccttga agcataagct ttggtgtgcc gattacatta
     1021 accgttactc tgctgcttac gtgagtgttg cctgccgtat cttttaccct tacatacaaa
     1081 tccttgcttc ctgcatcttc gggtccaggg aaccaggaat atgaaccata acctgattta
     1141 aacaatagag tttcttcacc ggttgaattg tcaaccatta tatattcggt atcagttaca
     1201 ttgaaattcc tttgtgccag taaagttaca gagccatcaa tagtttgtcc atttttaact
     1261 ccgcctaaat atagatatct gtcaacgtca attccaatgt ttacatattc gcccttatat
     1321 gcatttccct tagtatcgta agcaataacc cttacggaca tattgccgtt gtcttccata
     1381 acgggaatca tagtgaatga ttgctccggg tcaacaccgg gagacagata gacagctccg
     1441 ttatcaggat ttatcatttc atattttaca tatgatgccg agaaatttaa ctttgtgctt
     1501 aaaggtaccg aatgagctgt aattaattga ccttccctta ttcctttcag ctctatctga
     1561 ggatttattt ttaccgtaac aggtatcgaa tcacctgcca agaagtttcc ctgtgcatca
     1621 taaaatgcgg aaacaagtac cttctgccca ttgtcttcca ttgcaggaac ccattcataa
     1681 ggggtattta tgttgctgcc tcctgcaata acaaatcctt ttgccgtatc tctatccaaa
     1741 agaagatatt taatttcttt tgctcctttc ggtatttcat tattaatttt tgtatacagc
     1801 tcagttgttc cggtaatggt ttgtccaggt tttaccgatg aaatttccac atcaaaaaac
     1861 ttcgtaactt ctgagctttc cgatgaatct atatataccg ttcttttatc attgtcccat
     1921 tctatcttca cacccaaggc attgcttatt aaacgtatgg gaacaaaggt acggtctccg
     1981 gttatttgag gagtcacatc aataattgta taagaagttt cattgtcatt tgtatattca
     2041 actaagtagc tgtcaattct aagaagtatg tgtatatctt ctttagatat atggactgcc
     2101 ctgttatcat tatcccattc aacgacagaa tccaattctt cagcaataac ccttatgggc
     2161 accaatgtcc tgtcattgct tatataagga tccacatcgg gttttattct cttgccatct
     2221 attactattt taattgaatc ttcagcataa gcgtcgggtg ccgaatattg aaccaaagca
     2281 atcaatatta acaacagaat aagaattggc catttaatat atttaattct catcgcccac
     2341 ctctttattt ctttttttac attataccat gaaaaccgac ataataaaca ttaacattat
     2401 attacaatat ctcctataga gtttgtccct tctgtcaaaa aaatattgtt agggcgaagt
     2461 tacttccaac agagacactc ctacccacaa ttttaaaata tcagacagca caagtttatt
     2521 aacggttagt tgatattctg tatcgtcaaa tcttaatatt cttttatcta taacctgcac
     2581 aagtatatct gcatccaaac ttttgatttc cttgcccaag gatgaaacat atttttcttt
     2641 aattgctttt tcaatttcaa tattgtctat caggtctatt tgttccttgc ttaaaccggt
     2701 aaattccaat atgacggaaa tatccttcag cactatattt gcattgttga ttgaactttc
     2761 aagcttttcc agtttctcat ctttgtcttg aatggtatgc tccaaatatg ctatcttttc
     2821 ataaagctct tccattctat ggctgactaa tatacttata cccaaagagc ctataactgt
     2881 tccccaaagc agtcccgtta taaagcaaat aaaatattta tatcctgcag tgttttttac
     2941 atttccttta accctcatca tttcccccat atattgccgc atctttgaag aagcctgata
     3001 aattcataac ccatatttgc gccggttaat gaaatcacta tataaaatac ttgttttaac
     3061 aatgttttta tttctccttc aaacagccct ttttcaatta ccaagaaaga tgaaaaggtc
     3121 ccacccaggg atgccgctat agcccataat ttgattgaac ctgcaacatc tgtcatcacc
     3181 tttaaagggg gtgtgcctgt cataagagct cccaacccgg aaaacaagct tgctcctata
     3241 acgactccaa atgcaattaa aaagttatgc actatacttg aataaaaaga agacatttta
     3301 tcaccatact ctctttttat aatatgagtg aaaaatgtct ttatgattga ttgtttagtt
     3361 tcttcaatga gttcagagtt acgaggtctg agccttcaaa tcgaattcat tacaataaca
     3421 gtgtatttat ttatattctt cgtatgttct gacagacata agaattgctg cttctcttga
     3481 tgccatgccg taaccgacag ctttttgagc atccgtcaca gccttaggca taaaattacc
     3541 gttactgtca ccttttatta ttcccagttt agccataaat ttggttcctt ccactgccca
     3601 tgatgaaata tctgcttggt caggaaagtc cttaacacct tcaatactaa agtcaccttc
     3661 aggctctatt gcttttattg ttctaaaaag catggttgcg cattgttctc gattaattaa
     3721 ttcatttggt gaaaatgtag ttgctgatgt tccttgagta attcctatag aaaatgcttt
     3781 tacaatctgg ggattggtcg tatcggtaaa tggattttca gatacgcttg ccactgtctt
     3841 gtcagtagtt ttttcgtaaa gcaagagagc cagctctgca aattcttcac gggttattga
     3901 ttttgtcaaa tcttttccct tcaatatatc aggaataagt cctgcttcat tggctttttc
     3961 caattcaact actgcccaat cgctggcatc gtcataaaat gccgggttac ctattgtaac
     4021 tatgtttgag tactctgaat aaatatcttt gtcaatccaa tcgccctgat agccccaaag
     4081 tgcattgaat cgagcacgga actgataaac ttcgtttttg atatcaacgg tatcaaaatc
     4141 tccttcatca aatggttcat attcataagc accaagttca agataatcag aaacagccat
     4201 tgaagtattc cagtataatg agggaccttc attccaatcg taattgccat atttgtaatc
     4261 aaaaataatt tcaatcccat cgcaacttgt accgtcataa taatcaggat tatctaccaa
     4321 attagaatta attgttttta cactttcggg aacggtaagc tttaattcaa aataaggaat
     4381 accgtcatcc ctaattttta attcgcctgt aagatttttt ggtgcttcca aagaaaaagg
     4441 ctcgcttgcc aatgctgtca atggaaataa tgtcaataaa agtgccagag tcaaaaagac
     4501 agataaaaat tttttcattg taaccccctt ataattattt atattattta taatatttat
     4561 attaattata ttggaatcaa caattatttc aatcaccctg agggtgattt ttcctattaa
     4621 tcacccctgt tcttttatta aacccaatct gtatgcatct aaaagcattt ccaaatcttt
     4681 tatctgggcc ttcataactt ctcccgcatc ggtatccttg actctttttc tttctacctc
     4741 tttttccaaa accatagttg ttgaatgaat atctttttct tcttctatag ctttttgcgt
     4801 tgattcaaaa gggtcatgag ataccaagag cagtccgtag gaattatata tgagagtgta
     4861 tcctgcaatt ccggtagtac tttgatatgc tcttgaaaag cctccgtcta taactattaa
     4921 ttttccgttt gcttttatag ggctttctcc acttttgctt ttaaccggta catgaccgtt
     4981 aataatgtga gagcactcag gattcaagcc gaattcctcg aaaatccttc tgcacatttc
     5041 ttcactgtct tctaatttaa aataagggct tttagtttcc ttgtgtgttt tcttatcatc
     5101 tatgaaatat ctttcaaagg tagtcattct atctttgccg aacaaaggag aatcaggacc
     5161 ggtccacaag taccaggtaa tatccatgcc gtattgttta gcatccggat ttgttttgtg
     5221 aaaatatcct tccctgacta atatttcaag tctgtcaaaa tattcttttc cgctgtatgc
     5281 ctttcctgta cgaccgactg ttaccttctt aaaggtaccg tcttcattaa cgggaataca
     5341 cccgtgatat aaaaggttcg aatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnttaaagg taccgtcttc attaacggga atacacccgt gatataaaag gttcgaattg
     5521 aatttcagat acatgcttcc gtttgaaagc ataaatctta tatgtttttg taatttttcg
     5581 ctgtttaaaa aagatgactt tatcttttca accaattctt cttcttcttt ggtaagcgca
     5641 tacgggtttt tagggtcaat tgtcggaaag tttctatcat tcaagtgata tgtcttgcca
     5701 tataaatcaa ttgttccttc ttcataattt attttttcaa gcagcagcct gtcatccatc
     5761 ttaaaatacg gacgtctttt tattatgtga ccttccagct taaactgtat gatggcaatg
     5821 gctttatgca tttttgcaat tagttttaaa tccttatctg aataaacgat atcactttct
     5881 accttaggtt taaactgaat gcactcatca tcaccgtaat attccagtgc aaatgttgct
     5941 aagggaagaa catttatgcc gtatccgtct tctatgatgt ctaaatttgc atatctggca
     6001 catattctta atactgtagc aatacatgct tcacttcccg cagcagcacc catccacaat
     6061 acatcatggt tgccccattg tatgtccaca gtatgataat tcataatagt atccattact
     6121 ttatccgcac cgggacctct gtcaaaaata tctcctacaa tatgaagcct gtctataacc
     6181 aatctttgaa taagctttgc cattgcaata ataaattcat cagctctgcc tattcttatt
     6241 atcgtattta ttatttcgct gtaatattct tctttatctt cttcaggccc tttatgcatc
     6301 agttcttcga ttatgtatgc aaactctttc ggaagggctt ttcttacctt catgcgagta
     6361 tacttgaaag ctgctttcct gcaaatcacc acaagcctgt aaatagtaat tctataccag
     6421 tcttcaatgt cttcttcttc cttacgaatt atttctagtt tctgttcggg ataataaatc
     6481 aatgtggcaa ggcttttaat ttccttctca ctcattatat ccccgtaaac ctcgttgatt
     6541 ttacgcttta tattgcctga gccgtttctt aaaacatgat tgaatgattc gtattcccca
     6601 tgtatatcgg atagaaaatg ctccgtacct ttgggtagat ttaaaatagc ttgtaagttt
     6661 ataatttctg tacatgcaga agctattgtc gggtattgtt ttgataaaag ctttaaatat
     6721 ctaatttcct catctaacat acgaattgca tcatcattag aagcagtcat gatatttctc
     6781 cttttattat atttccacaa cggatattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnat aataggtttt gtaaattcca aaaattttga tataatcaaa gaaaaagaat
     6961 atagttgtac ctatatcggg aggacttatg aaaaactcgt ataaaatttt agctattaat
     7021 ccggggtcta cctctacaaa aattgcagta tatgaaaatg agaaattaat atttaaagaa
     7081 acaatatctc atagcaattc agatttggag aaattcgata aaattactga ccagtttaaa
     7141 tatcgaaaag atataatatt ggacacgata aaaaaacata actttgacct taatgaactg
     7201 gatgcaattg taggcagagg cggtaacttt aagcctgtag aaggcggcac ttacaaggtt
     7261 aataaagaaa tgattaatga tttaataatt ggtgttatgg gacaccatgc ttcaaatctg
     7321 ggtgccataa tagctgatgc aatagccaat gatttgggat tatcagcata tatcgttgac
     7381 ccggtagtcg tagatgaact tgaagattat gcgagaattt cagggatgcc tgaaatagaa
     7441 aggaaagcca aagctcatcc tttaaatcag aaggcagtag ccaggctggc agccgccgaa
     7501 ttaggaggaa agtattccga ttataatttt gttgttgcac acatgggtgg aggtatttcg
     7561 attggagcac acaaaaatgg aagaatagta gatataaata attgtttgga tggcgaagga
     7621 acatttactc ctgaaagagc gggaggatta ccggtaggct cattagttga aatgtgcttt
     7681 tcgggaaagt acacaattca agaaataaag aaaaaaataa ccggcaaggg cggcttggta
     7741 gcatacttag gtacaaatga tgccatggag gtaaaaaaga gaatagcagc aggagataaa
     7801 tatgcaaaac ttatatatga aactatggca tatcagattg caaaagatat cggagcagca
     7861 gcaacggttc tcaaggggaa aattgataat ataatattga ccggcggatt agcacatgat
     7921 gatgtcctgg ttcaatggat aaaagaaaga gttgaattta tagcgcctgt aaaggtatat
     7981 cccggagaat ttgaaatgca cgcaatggta caaggtgtta tacgagtatt aaccggagaa
     8041 gaagagttaa aaatttacag atgattaaaa cccactatga tgtgggttaa ttattctttt
     8101 aaactctcta tatccaacaa attataatca aacaggattt cttctccgtt ttcgattacg
     8161 atgcaaggta acccaaccct gccttcatct ttaatttcct tgaattcagg cgcattgtcc
     8221 ctgtatttca aaaatcgttt aagattaatc atattttctg atatatccag atatatgtaa
     8281 ttaacttcat tttccgaaag aaactctttc gcaggttcac aatccggtca atgtttgctt
     8341 ccaaacacaa ttactttctt cattcgcttc tccttatatt aaatttacca taaaaataga
     8401 atgttattag taattaacac ctctctaaca aatttctatt tttgtattat ttatcattat
     8461 aagacaattg ataaaattat acaataataa atgcttcaat tataaactta acacgaatgc
     8521 tggcaatcag ggcaatagaa gcatttacga gaattgagtt ctattttaac aatagtacct
     8581 ccacatcgac tacatttctt gccctctctt ccataaacaa gagtatgagc attgtatccc
     8641 cctgtatgtt gatcccctaa aaataaagga ttatccatat acccgccaag agaaatagca
     8701 ttctttaaaa cagtttgaat ggaattataa accgagaaca gaccatcttt accaagagaa
     8761 tctactgtca cagtcggctt gagctttgct tcaaagcaaa tttcatcggc gttctatctt
     8821 tgttatctgt tttcccctta cttcctgaac aaattccaaa cttggtatat taattgactt
     8881 ctccctgtta atttcaacat cagtaatagt ctttcctaaa atctttttat caagtaattt
     8941 tcgatatgtt tccatttccg gaagctctgg catagtgtta ttctccttca tgttaaactg
     9001 taagtagtat tggcatttaa tgatttaata tttctaaaag attaaatcaa atattaatta
     9061 caaatgtttt tacatattgc cttattgttt ggttacatgt cggaaaacaa aaaagacacc
     9121 aaccctccgg ttggtgtccc tcgtcaagtg gttcttacat attgctgact ttttcctggc
     9181 tctgtgcttc tttatatttt aagcgagtag ctttacctcc cctaatgtgt ctttcagact
     9241 tatttttaag caaaactttc t
//
LOCUS       DAOSHP010000070         8469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7152_length_8469_cov_9.234371, whole genome shotgun sequence.
ACCESSION   DAOSHP010000070 DAOSHP010000000
VERSION     DAOSHP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7152_length_8469_cov_9.234371"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLB33_07195"
     CDS             <1..1136
                     /locus_tag="PLB33_07195"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOG63347.1"
                     /translation="TKYEEKLNWLRQMLEWQQETNDPQEFMEALKIDLYTDEVFVFTP
                     KGEVINLPTGSTPIDFAYRVHTAVGNKCVGAKVNGRIVPLDAKLKTGDQVEILTSQSS
                     VGPSRDWLKIAASSQAKSKIRKFFKDRDKDFNVEKGKELVEREIKKQGYQVADLLKDD
                     WIKTVADKYNMSAASNLFAAVGSGGITESQIVNRLKLIYLEKNKDKLELDRIENLEKS
                     LAKSPQRERHTSGVIIKGIDNIKVRFSKCCNPVPGDAIVGYITRGRGVSIHRADCTNI
                     HDLSDSDDQRFIEVQWDNKEKATFISELQIKALDRPKLLQDITSLYVDAKLNAVSLNL
                     RINKEKVAIVDIGFEINDIKQIEDLIKKMKKLDGVLEVYRNKK"
     gene            1159..1608
                     /gene="dtd"
                     /locus_tag="PLB33_07200"
     CDS             1159..1608
                     /gene="dtd"
                     /locus_tag="PLB33_07200"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG63348.1"
                     /translation="MRAVVQRVKSAHVKVKDKIIGSIGQGILQFLGVEETDQETDLEY
                     MCEKIPNLRIFEDENGKMNKSLLDVEGSILVISQFTLLGDARKGRRPSFTDAAKPEKA
                     IPMYEKFIACMKEKNITTQSGEFGAEMEVELINDGPVTILLDSKKVF"
     gene            1618..2238
                     /locus_tag="PLB33_07205"
     CDS             1618..2238
                     /locus_tag="PLB33_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOG63349.1"
                     /translation="MIFEALEVGIYCANCYIVGSEETKEAAIIDPGADFNRIEGKLHE
                     LGLTPKIIILTHHHGDHIGAVKEFTDKYGLKVYIHKEDAEALNNSSMNFTKSMFGKNI
                     LIKADVELEDGDVIYLGELKFEIIHTPGHTKGGICIKVGNIMMTGDTLFNSSIGRTDL
                     PGGSYEEIISSIKEKIFKFDDDTVIYPGHMSPSTIKKEKKFNPFVK"
     gene            2258..3724
                     /gene="hemZ"
                     /locus_tag="PLB33_07210"
     CDS             2258..3724
                     /gene="hemZ"
                     /locus_tag="PLB33_07210"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HOG63350.1"
                     /translation="MFKFYFFGHDYEYEARNALRVFDLNIDYEIMHGSFDGRSLSVLS
                     YLEEGEIISAKAQLYNDCELLYESCISENDIVLEKYSLKKLKKTLVIKTIHNVLKSYY
                     KVYPDYGILTGVRVVKILITAKKYSKSNEEINKILRDTYEVKEEKIKLLWDILNIEEK
                     YIDENINKKNYNLYIGIPFCPSKCSYCSFTSYVNCTDKKIFTYLDTLIYEIEKTIELA
                     KKKELNLHTIYIGGGTPSVLNEEQIETIFNAIKKYYDLSQVKEITFEAGRPDTINSEK
                     LICLKNNFVNRISINPQTMNDNTLLSIGRKHLGFEVVEKYLLAREIGFQTINMDIILG
                     LPGEDESDVKNTIEIIAGLNPENITVHSLAYKKHSELTRESAKLFKDYELIKKMHHVI
                     KEVCEVKCYKPYYMYRQKNIKGNSENIGYTKKDRECIYNIVMIEELETILGCGLGASS
                     KIMTGENKHETVRNFKSLEEYSKRIDEIINKKQTLLGG"
     gene            3725..3985
                     /locus_tag="PLB33_07215"
     CDS             3725..3985
                     /locus_tag="PLB33_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG63351.1"
                     /translation="MKKIKLFYIEGCPHCRKAFRILDELKAKNPEYSKIDVEYIDENK
                     DVVSANLYDYYYVPTFFVDDVKMHEGVPTEEKIERVLKEAIK"
     gene            complement(4030..4359)
                     /locus_tag="PLB33_07220"
     CDS             complement(4030..4359)
                     /locus_tag="PLB33_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOG63352.1"
                     /translation="MEHFMETAVHYVAYTLEIVSIIIVAYSAVTTLIKYLMSKFEFTN
                     KQAKIEFSRALEMALAYMLGAEILKSLIAHSKEQLITLAALVLLRAAIALIIHFETEA
                     DAKHCEK"
     gene            4585..5349
                     /gene="pdaA"
                     /locus_tag="PLB33_07225"
     CDS             4585..5349
                     /gene="pdaA"
                     /locus_tag="PLB33_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HOG63353.1"
                     /translation="MIVVLLFACLLIYNTFKNFSVTVSTNISNWGLKFNENGTAPMGN
                     AGKDFLKKYNAYYVGEPDKKYIYLTFDAGFENGNTPNILDTLKKHDVKAAFFLVGHYL
                     EKEPGLVKRMVREGHIVGNHTYHHPDMSLISDKESFRRELTSLEELYKEITGEEMPKY
                     YRPPQGKFSEENLRMADELGYKTIFWSLAYVDWYKDDQPTKEQAFSKLIPRIHPGAVV
                     LLHSTSDTNEMILDELLTKWKDLGYTFKTLDDLTAN"
     gene            5702..7474
                     /gene="aspS"
                     /locus_tag="PLB33_07230"
     CDS             5702..7474
                     /gene="aspS"
                     /locus_tag="PLB33_07230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOG63354.1"
                     /translation="METMGTLRRTNMCGELNKEHIGKEVVLMGWVQKRRSLGGLIFAD
                     LRDITGLIQIVFDADVNKEAFEKADSLRSEYVIAIKGKVFERQSKNLEMATGEIEVYA
                     QELRILDTAETPPIYIKDNDDVSEQMRLKYRYLDLRKPSMQKNLLMRSKTSKVIRDFL
                     YENGFNEIETPFLAKPTPEGARDYLVPSRISPGNFYALPQSPQIYKQLLMVSGMNRYF
                     QIVRCFRDEDLRANRQPEFTQVDIEMSFVDAEDVMAINERLIQRIFKEVKNIDIKLPL
                     RRMSYNEAMDKYGCDKPDLRFGYELKNITYVFKNSEFNAFKSVYENSGLIKCIKIDGA
                     SDDYSKKGISKLEKFVKSHGAKGLAWFKYENGLFDSPIAKFLSEGEINELISLLELKE
                     KDIVFIVADNESAVNTALSALRIEIAEERNLIDNNVYELVWITDFPLFEYSEEEERYV
                     AKHHPFTSPVDEDIDKLESAPEEVRAKAYDIVINGDEIGGGSIRITNRDLQNRMFKAL
                     GFTEEEAQKKFGYLLEAFKYGVPPHGGIAYGLDRFMMLLTGANSIKDVIAFPKTQSAS
                     CLMTESPSQVSEKQLDELKIEINE"
     gene            7497..7919
                     /locus_tag="PLB33_07235"
     CDS             7497..7919
                     /locus_tag="PLB33_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOG63355.1"
                     /translation="MDNIGKIEKIDGNMAVISVKRVSSCGDSCKSCSSACKVNSVIIE
                     TDISDVKDEINVGDYVEIRAENEVMLKHIAVLYGLPLLLMLVTVFIFQMILKGPNKDI
                     ISAVASLFSLIISFYLLKAYDKSEMKKNVLKFTVGKKL"
     gene            8016..>8469
                     /locus_tag="PLB33_07240"
     CDS             8016..>8469
                     /locus_tag="PLB33_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG63356.1"
                     /translation="MNIFEMQYQNSIKKEGPLAERMRPLNLSEFVGQEHIVGEGKILN
                     RAIEADKLTSAIFYGPPGTGKTTLAKIIANTTNNNFTTLNAVTSGIKDIKEKISYAVD
                     ELSIYNKRTILFIDEIHRFNKAQQDALLPYVENGTIILIGATTENPYFE"
BASE COUNT         3175 a         1195 c         1732 g         2367 t
ORIGIN      
        1 aaaccaagta tgaagaaaag ctgaattggc tgaggcaaat gcttgaatgg cagcaggaga
       61 ctaacgaccc tcaggaattt atggaagctt tgaaaattga cttatatact gacgaagtat
      121 ttgtatttac tccaaaggga gaagtgataa atttgcctac aggatccaca cccatagatt
      181 ttgcttacag agttcatact gcagtaggaa acaagtgtgt gggagcaaaa gttaacgggc
      241 gaattgttcc gctcgatgcg aagcttaaaa caggtgacca agtagaaata ttaacatccc
      301 agtcaagtgt gggaccaagc agggattggc tgaaaattgc cgcgtcaagt caggcaaaat
      361 ctaaaatcag aaaatttttc aaagatagag ataaagattt taatgttgaa aaaggaaaag
      421 aattagtaga aagagaaatt aaaaagcagg gataccaagt agcagatttg cttaaggatg
      481 actggattaa aactgttgcc gacaaataca atatgtctgc tgcaagcaac ctctttgcgg
      541 cagtaggcag cggaggtata acagaaagcc agatagtaaa caggcttaaa ttaatatatc
      601 ttgaaaagaa caaagataaa ctcgaacttg atagaattga aaatttagaa aagagtttgg
      661 caaaatcacc ccagagggaa agacatacat ccggcgttat aataaaaggt attgataata
      721 taaaagtccg tttttccaaa tgctgcaatc cggttcccgg agatgccata gttggatata
      781 tcacaagagg cagaggagta tcaattcaca gggctgactg cacaaacatt catgatttaa
      841 gcgattctga cgaccagaga tttattgagg ttcagtggga taataaagaa aaggcaacat
      901 tcatcagcga actacagatt aaagcgttgg acagaccgaa acttttgcag gatataactt
      961 ctttgtatgt agatgcaaag cttaatgccg tatcgcttaa tttaagaata aataaggaaa
     1021 aagtagcaat cgttgatatc ggatttgaaa taaatgatat taaacaaata gaagatttga
     1081 ttaaaaagat gaaaaagtta gacggagtat tggaagtcta cagaaataaa aagtgacgaa
     1141 aaaatgaaag gatgaaaaat gagagcagtt gtgcagcgcg taaaaagcgc ccacgttaaa
     1201 gtaaaggata aaataatagg aagcatcggt cagggaattt tgcaatttct cggtgttgaa
     1261 gaaacagacc aagaaacgga tttagaatat atgtgtgaaa agattccaaa tcttagaata
     1321 tttgaagatg aaaacgggaa aatgaataaa agcttgttgg atgtggaagg aagtatattg
     1381 gttatttcac agtttacatt actgggggat gcaagaaaag gaagaaggcc aagttttact
     1441 gatgcagcca aaccggaaaa ggcaattcct atgtatgaaa aatttattgc atgtatgaag
     1501 gagaaaaata ttacaacaca atcaggggag tttggtgctg aaatggaagt agagttaata
     1561 aatgacggtc ctgttaccat acttcttgac agcaaaaaag ttttttagga ggttcttatg
     1621 atatttgaag cattggaagt cggtatttat tgtgcaaact gttatatagt cggaagtgaa
     1681 gaaactaagg aagcagccat aatagacccc ggtgctgact ttaatagaat tgaagggaaa
     1741 ctgcatgaat tgggattaac ccctaaaata ataattttga ctcatcatca tggtgatcat
     1801 attggagcgg tgaaagaatt taccgataaa tacggattga aagtttatat tcataaggaa
     1861 gatgcggaag cactcaataa cagcagcatg aatttcacta aatcaatgtt cgggaagaat
     1921 attttaataa aggcagatgt tgagttagag gatggggacg taatatactt aggtgaatta
     1981 aaatttgaaa taattcatac tcccggacat acaaagggag gtatttgcat caaggttgga
     2041 aatataatga tgacgggaga cactttattc aactcttcca ttggaagaac cgaccttccc
     2101 ggaggctcct atgaagaaat tataagctca ataaaagaaa aaatatttaa attcgatgat
     2161 gatactgtaa tttatccggg tcacatgtca ccttcaacaa taaaaaaaga aaagaaattt
     2221 aatccgtttg taaaataaga tgctatcagg tagaaaaatg tttaagtttt atttttttgg
     2281 tcatgattac gaatatgaag ctagaaatgc gctcagagta ttcgatttaa acatagatta
     2341 tgaaataatg catggctcct ttgacggaag gagcttatct gttttatcat atcttgaaga
     2401 aggcgaaatt attagtgcaa aggcacagct ttataatgac tgtgaattgc tgtatgaatc
     2461 atgtatcagt gaaaatgaca tcgttttgga gaaatacagc ttaaaaaagc tgaaaaaaac
     2521 attggtaatt aaaactatac acaatgtttt gaaatcatat tacaaggtct atcccgatta
     2581 tggaatatta accggagtca gggtggtaaa aattttaatc acagcaaaaa aatactctaa
     2641 aagcaacgaa gaaataaata agatactgag agatacctat gaagtgaaag aggaaaaaat
     2701 aaagcttcta tgggatatat taaatataga agaaaaatat attgatgaaa atataaataa
     2761 aaaaaattac aacttataca taggtattcc tttttgccct tctaaatgca gctattgctc
     2821 ctttacctca tatgtaaatt gtactgataa aaaaatattt acctacttgg atactttgat
     2881 atatgaaatt gaaaaaacaa ttgaacttgc aaaaaagaaa gaattaaact tacatacgat
     2941 ttatataggc ggcgggactc cttcagtatt aaatgaagag cagatagaaa caatttttaa
     3001 tgcgataaaa aaatattacg atttgtctca agtaaaggaa attacttttg aagcaggtag
     3061 accggatacc ataaatagtg aaaaacttat ttgtcttaaa aataattttg taaacagaat
     3121 atccataaat ccccaaacga tgaatgataa tacattatta tcaatcggaa gaaaacactt
     3181 gggctttgaa gttgttgaaa agtacttatt agctagagaa atcggatttc aaacaataaa
     3241 tatggatatt attttgggac tgccgggaga agatgaatca gatgtaaaaa atacaattga
     3301 aataattgcg gggttaaatc cggaaaatat tacagtccac tcattggcat ataaaaaaca
     3361 ctctgagctt accagagaaa gtgcaaaact ttttaaggat tatgaactta taaaaaaaat
     3421 gcaccatgtt ataaaggagg tttgcgaagt taaatgttac aaaccatatt acatgtatag
     3481 acaaaagaac atcaagggca actccgaaaa catcggctat acaaagaaag acagggagtg
     3541 tatttacaac attgtaatga tagaagaatt agaaacaata cttgggtgcg gtcttggtgc
     3601 ttcaagcaaa ataatgacag gtgaaaacaa gcatgaaact gtacgcaatt ttaaaagcct
     3661 ggaagaatat tcaaagagaa ttgatgaaat aataaataaa aagcaaactc tattaggagg
     3721 ataaatgaaa aaaataaaac ttttttatat agaaggctgt cctcattgca ggaaagcatt
     3781 tagaatactt gatgaattga aagctaaaaa tcccgaatac tcaaaaattg atgtggaata
     3841 catagatgaa aacaaggatg tagtatcagc aaatttatat gattattact atgttccaac
     3901 attttttgtg gacgatgtga agatgcatga aggggtaccg acggaagaaa aaattgaaag
     3961 agtattgaaa gaggcaataa aataaatcaa aaaatcagga cagctcgtcc tgattttttt
     4021 tcaataaaat tatttttcac agtgctttgc atctgcttct gtttcaaaat gaataataag
     4081 tgctattgcc gctctcagta atactaaggc tgcaagtgtt atcaattgtt cttttgaatg
     4141 tgctattaaa gatttaagaa tttccgcgcc cagcatatat gccaaagcca tttccaacgc
     4201 ccttgaaaat tctattttag cttgtttatt agtaaattcg aatttgctca ttagatattt
     4261 tattaatgtt gttactgcac tgtaagcaac aattattatg cttacaattt ctaaggtgta
     4321 agcgacataa tgaactgcgg tttccatgaa gtgttccaat taaaaacctc caaatttata
     4381 ttatatttaa cgtatgctat tatattgcat tttattaata atgtcaaagc ccttttagag
     4441 tgattttgga aatatgcatc ctattataaa aaagaataat aaataattta tagccagtca
     4501 tattaagcct gtttatttat ataaataaat aaaggggtga gatatatggt attaagaata
     4561 aacaaaaagg tatttattat tataatgata gtagttttat tgtttgcatg tttattgatt
     4621 tacaatactt ttaagaattt ttcggtgaca gtttcaacaa atatatcgaa ttgggggcta
     4681 aaattcaatg aaaacggaac agcaccaatg ggtaatgcag gaaaagattt tttaaagaaa
     4741 tataatgcct attatgtagg ggagccggat aaaaagtata tttatctcac atttgatgcc
     4801 gggtttgaaa acggaaatac tcccaatata cttgatacat taaaaaaaca tgatgtgaag
     4861 gctgcatttt tcttagtggg acactatttg gaaaaagagc ccggacttgt caaaaggatg
     4921 gttagagagg gtcatatagt aggtaatcat acctaccatc atcccgatat gtcattgatt
     4981 tctgacaagg aatcatttag gagggagtta acatccttag aagaattata caaggaaata
     5041 accggtgaag agatgccaaa gtattacaga cctcctcaag gcaaattcag tgaagaaaat
     5101 ttaagaatgg ctgatgaatt gggatataaa acgatttttt ggagccttgc gtatgttgac
     5161 tggtataagg atgaccagcc taccaaggag caggcattca gcaaattaat tccccgaata
     5221 catcccggag cagtagtctt attgcactct acctccgata caaacgaaat gatattggat
     5281 gagctgttaa caaagtggaa agatttagga tatacattta aaacattgga tgatttaaca
     5341 gcaaactaaa tgacaagggg acgggggtac tgtcatcaaa atttaatttt attgttgaca
     5401 aacaagatgg ggtgtggtaa tttagttgta aataattgca ggggaagagt agtcataaag
     5461 caatgattta agcaagacag ggacgatggg agcctgttat caacttttga tgaagacacc
     5521 tcaaaaatgc aaattctaat gagtgggact tattagtcca aatagggtgg caacgcgggt
     5581 tatactcgtc cctaagcagg ggacgggttt ttattattat aggtattggg acacacctct
     5641 ttcaataaag attgcctttg ccagtcagag gaatgtccct acttaaacaa tggaggatac
     5701 tatggaaacg atgggaacac ttagaagaac aaatatgtgc ggagagttga ataaggaaca
     5761 cattggcaag gaagtcgtgc tgatgggatg ggtacaaaaa agaaggagct taggcggact
     5821 tatttttgcc gacttaaggg atattaccgg attaattcaa attgtttttg atgctgatgt
     5881 aaataaggaa gcttttgaaa aagcagatag tttaagaagt gaatatgtaa tagcaattaa
     5941 aggaaaagta tttgaaagac agtctaaaaa ccttgaaatg gctacgggag agatagaagt
     6001 ttacgcacag gagctaagaa tattagatac tgctgaaact cctcccattt atataaagga
     6061 taatgatgat gtttccgagc agatgaggtt aaaatacaga tatcttgatt tgagaaagcc
     6121 ttcaatgcaa aaaaatctat tgatgagaag taagacatca aaggttataa gggacttttt
     6181 atatgaaaac gggttcaatg aaatcgaaac accattttta gcaaaaccta cacctgaagg
     6241 agcaagggac tatctggtac caagcagaat ttcacccggt aatttctatg cattgcctca
     6301 atcgccgcaa atttataagc agcttttgat ggtttcggga atgaacagat atttccagat
     6361 tgtaagatgc ttcagagacg aagacttaag agcaaaccgt caacctgaat tcacacaggt
     6421 agacattgaa atgtcctttg tggatgcaga agatgtaatg gcgataaatg agaggcttat
     6481 tcaaagaata ttcaaggaag taaaaaatat cgatattaag cttccattaa gacgtatgtc
     6541 ttataatgaa gctatggata aatacggctg cgacaaacct gatttacgtt ttggatatga
     6601 gctgaaaaat attacttatg tgtttaaaaa ttctgaattt aatgcattca aatcagtcta
     6661 tgaaaacagc ggcttgatta aatgcataaa aattgacgga gcatcggatg attattcaaa
     6721 gaaaggcatt tctaagttag agaaatttgt aaaatcacac ggtgcaaaag gattagcctg
     6781 gtttaaatat gaaaacggcc tatttgattc tccgattgca aaatttttaa gtgaaggcga
     6841 aataaatgaa ctaatttctt tgttggagct aaaggaaaaa gatatagttt ttatagttgc
     6901 cgataatgaa tctgccgtaa atactgccct cagcgccttg agaatcgaaa tagccgagga
     6961 aagaaattta attgacaata atgtttatga attagtgtgg attactgatt ttccactgtt
     7021 tgaatacagc gaagaagaag aaagatacgt ggcaaaacac catccattta catcacctgt
     7081 agatgaggat atagataaat tagaatcagc tccggaagaa gtgagggcaa aagcatatga
     7141 catcgtaata aacggtgacg agattggcgg cggaagcata cgtataacaa acagggattt
     7201 gcagaacaga atgtttaaag cattaggatt tactgaagaa gaagcacaaa aaaaattcgg
     7261 ttatctgctt gaagcattta aatacggcgt gcctcctcac ggcggaattg catatggatt
     7321 ggaccgcttt atgatgcttt tgacaggcgc aaacagtata aaagatgtaa ttgcatttcc
     7381 aaaaacacaa agtgcttcct gcctgatgac agaatctccc agccaggtat ctgagaagca
     7441 gcttgatgaa ctgaaaattg aaattaatga gtaagtttta taggaaggaa gatcaaatgg
     7501 acaatatagg aaagattgaa aaaattgacg gtaacatggc tgttatttct gttaaaaggg
     7561 tatcatcctg cggggacagc tgcaaaagct gctcttccgc atgcaaagta aacagcgtta
     7621 ttatagaaac cgatatttcg gatgtaaaag atgaaataaa tgtcggagat tatgtggaaa
     7681 ttcgagcgga aaatgaagtg atgcttaagc atatagcagt tctttacgga cttcctttat
     7741 tattgatgtt agtaactgtt tttatttttc aaatgatatt aaagggtccc aataaggata
     7801 taatctctgc agtagcttca ttgttttcgt taataatttc tttttacctc ttaaaggctt
     7861 acgataagag tgaaatgaaa aagaatgtat taaaatttac tgttggtaaa aaactttaat
     7921 gcatacgatc aggatgacaa aggtcatctg ggcgcagtga tgaatcccgt gaggcgtcat
     7981 tctgaacgca ttgaagaatc ataaggattg atacaatgaa tatatttgaa atgcaatacc
     8041 aaaattccat aaaaaaagaa ggtcctctcg cagagcggat gagaccttta aatttaagtg
     8101 aatttgtcgg gcaagagcat attgtgggcg aaggcaaaat tttaaacaga gcaattgaag
     8161 ctgacaagct tacctctgcg attttttacg gacctccggg aaccggcaag acaaccttgg
     8221 ctaaaattat tgcaaatacc acaaacaata attttacgac tcttaacgcc gttacaagcg
     8281 gtattaagga tataaaggaa aaaataagct atgcagttga tgagctttca atttataata
     8341 agagaacaat tcttttcata gatgaaattc atcgtttcaa taaagctcag caagatgcct
     8401 tgcttcccta cgttgaaaat ggtacaataa tattgattgg agcaaccact gaaaatccgt
     8461 attttgaag
//
LOCUS       DAOSHP010000071         8355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7297_length_8355_cov_8.500120, whole genome shotgun sequence.
ACCESSION   DAOSHP010000071 DAOSHP010000000
VERSION     DAOSHP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7297_length_8355_cov_8.500120"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1082
                     /locus_tag="PLB33_07245"
     CDS             234..1082
                     /locus_tag="PLB33_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOG63357.1"
                     /translation="MRRNKKSNIKKTLIAVTALLFILFAVLLIKEKLNNDKLPPQEVQ
                     EEENPPNNQNKPEDEEETSTEPEKEPQEEEPKEPEPEQVPEDTEYGIIIQNPEKIDVL
                     VNRKRNLPDTYVPEDLVTLSEVPTVLSNPEVNQLRSAAYEALKELFNAAWEEAGFELC
                     ARSGYRSYNTQSSLYTSYVESYGQQAADKYSAKPGQSEHQTGLAIDITCEALNYKLDT
                     SFAETEEGKWVSENAHRFGFIIRYPKGKEEITGYAYEPWHIRYLGVELAEEIYESGLT
                     LEEYFQ"
     gene            complement(1382..3253)
                     /gene="htpG"
                     /locus_tag="PLB33_07250"
     CDS             complement(1382..3253)
                     /gene="htpG"
                     /locus_tag="PLB33_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOG63358.1"
                     /translation="MAKKQFKSESKRLLNLMINSIYTHKEIFLRELISNASDAIDKRY
                     YMSLRDNNIDFNKDDFYIRITPNEEERILRVSDTGIGMSKEELDSNLGIIAKSGSFDF
                     KENNEIKEDVDIIGQFGVGFYSAFMVSEKVTVISKRADSEEAYKWESTGADGYTITPC
                     KKDSVGTDIIMKIKPSTEDEDYDEFLDAYNIKSLVKKYSDFIKYPIKMMMKKRKLKEG
                     TENEYEDYFEDEILNSMVPIWRKNKSELKEEDYHNFYTEKHFGYEKPLKYTHVSAEGV
                     VSYNAILYIPARAPYDFYTKEFEKGLELYSNGVMIMKKCGDLLPDYFGFVQGLVDSPD
                     FSLNISREVLQHDKQLQFIAKKIKEKIKSELLSMLKEDRETYEKFFINFGKVLKYGLY
                     SDWGLNKEFLQDLVMFYSSYEEKLTTLDEYVSRMKEDQKYIYYAAGESVDKLSKLPQA
                     ELIADKGFEILYFTDEIDEFAIKVLLNYKDKEFKNISSSDLDIKSEDDNTDNVEENKE
                     LFAFMKESLGEKVKEVRASKRLKTHPVCLATEGELSIEMEKVLNALPDKPSSIHAEKI
                     LEINTSHQIFEKLKDAFHNDKEKFKKLSNVLFNQALLIEGLTIEDPVQYANDVYELI"
     gene            complement(3468..4010)
                     /locus_tag="PLB33_07255"
     CDS             complement(3468..4010)
                     /locus_tag="PLB33_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOG63359.1"
                     /translation="MVTDLSFNKNLVMVIQNHLNNIKNLYPSLPSVTPTGIYDEATQN
                     AVLIFQKIKGLSPTGFVDVPTLNALVHENHECVKKTQTPYKISFSTRDFVDVKLGDQR
                     DIVYVIKIMINGFCRRYANYNKLEVTNVFDEETEEAVRLFQQRSMLPPSGIVDIKTWN
                     ALVKIYDVCRLYKENSCVRN"
     gene            complement(4023..5441)
                     /locus_tag="PLB33_07260"
     CDS             complement(4023..5441)
                     /locus_tag="PLB33_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOG63360.1"
                     /translation="MSIPEIDVVIPELPLPHVIVPFPLYITVHLGAPDDEEALNVTVP
                     YLEYIKNVASSELYPTWPEEALRANIHAITSFAMNRIFTEWYRSRGYDFDITNTTQYD
                     QAYVHERGIFDTISNIANEIFNVYITREGHIEPLFAAFCDGRITQCDGMYQWGSVELA
                     NQGYTAEEILKYYYGEDITLVESTAAVEITGTYPGEPLSIGDSGIDVFRMQHSLNVIH
                     NNFPLIPAVRITGFFDEETENAVRIFQEAFNLPVTGIVDRDTWYRIRFIYIAVTNIAE
                     LVSAGLTYEELQELYANIVLQGATLPVVVYIEFFLNVLSQKYDAIEPVEIDTFYGPDT
                     DEAVRQFQIIMGLNPTGIVDQQTLNLLFREAYLILTETPVEELELPVLPFIGMELTEG
                     MGLEYPRILLLQIMLNAISAIHPEIRPVEIDGIYGPDTTAAVIIFQNLYGLPVTGIVN
                     EETWNKLNEVYQLSLLERETNT"
     assembly_gap    5482..5581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5582..6093)
                     /locus_tag="PLB33_07265"
     CDS             complement(<5582..6093)
                     /locus_tag="PLB33_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOG63361.1"
                     /translation="MSVTMIQQTYPESPLRLGDDNQHVLIAKMALNAIAVNYPAIPRI
                     SPVNSLFDENMEAAVKEFQNIFNLPVTGIIDLATWVEIGSKYATIVRLAEISTRGVLV
                     GDVVEDITEIEEGVQVVPRVQLVQYFLNVLSAYYDSIPAVDIDGILGPETRNAIIEFQ
                     KTMKLPLTGT"
     gene            complement(6103..6618)
                     /locus_tag="PLB33_07270"
     CDS             complement(6103..6618)
                     /locus_tag="PLB33_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63362.1"
                     /translation="MNRRGRNIPYVARNKIRKTEAPPELPLLNENQLNEDAAIQTGYV
                     NVSVNTALGALPVEDAVVTLYVTDQEGNQEALYHLVTDISGKVPRMALPVVYNPQDPS
                     ESREYFFSTYNLRIQAIGYYTQNILDIRVFPDTSTNFNVTLIPISQNGDEDEEERTLV
                     IPSSPIDRSNV"
     gene            complement(6704..6988)
                     /locus_tag="PLB33_07275"
     CDS             complement(6704..6988)
                     /locus_tag="PLB33_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOG63363.1"
                     /translation="MILLVRFEDIRLPLLPFLGVDLSEGMGPEYPRILLLEIMLNSIS
                     AMHPEIKPVEIGGIFDSDTRAAVIAFQSLYGLPVTGMADAQTFYHHLIIV"
     gene            7277..8170
                     /locus_tag="PLB33_07280"
     CDS             7277..8170
                     /locus_tag="PLB33_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG63364.1"
                     /translation="MISNEDLIRMHLINGLGRKTICKILACIESENIKLNNFSEVLKL
                     LSDMKIKRLKIDEEKIEKEASIIFECCKKNKIKIVGLYDEIYPYKFRLIEDKPLLLFV
                     DGDLELLNQANNIAIVGTRTPSQEGYEISSILAEKLTEEGCCIISGFASGCDEAAHYG
                     SLQANGRTIAVIPSGHINVLKQNRSLYNMIILNKGCIISELPPLAKADNNSFMDRNRL
                     IAAVSEGLIVIEGGLRGGTSHVVKFAKEYNKPVAYTANMCKITGQTLIFKDIDIIDSF
                     EKLVKFKSKSCNKILDKAIPQ"
     gene            8200..8282
                     /locus_tag="PLB33_07285"
     tRNA            8200..8282
                     /locus_tag="PLB33_07285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8234..8236,aa:Leu,seq:tag)
BASE COUNT         2589 a         1504 c         1447 g         2715 t
ORIGIN      
        1 taaaacctgt tgaggatgcc ttgataatgt ggaaacaaat agactgatga caaacattct
       61 aagcagaagc ctgtcattta tatgaataac atttttgtca ttctgagagc tttagctcga
      121 agaatctcat gagatctttc gcttactctc aggatggcat cccaaaatat aactgtttat
      181 cattcgttaa gcaagtgact atgaccggaa tgacattaac ataggagaaa acaatgagaa
      241 gaaataagaa aagtaatata aaaaagacat taattgctgt tactgctttg ctctttattt
      301 tatttgcagt attactgata aaggaaaaat taaataatga taaattacct cctcaagagg
      361 ttcaggagga ggagaatcct ccaaacaatc aaaataaacc cgaggatgag gaagaaactt
      421 caactgaacc ggaaaaagaa ccccaggaag aagagccaaa agagcctgaa ccggaacagg
      481 tgcctgaaga tactgaatat ggcataataa ttcaaaatcc tgaaaaaata gatgttctgg
      541 taaatagaaa aagaaatctg cctgatacct atgtgcctga agatttagta acgcttagcg
      601 aagtacctac agtgctttca aacccggaag taaatcaatt aagaagtgcc gcctatgagg
      661 cattgaagga attgttcaat gctgcatggg aagaagccgg ttttgaactg tgtgcacgct
      721 caggctacag gtcatataat acgcaatcat cattgtatac atcatatgtt gaaagctacg
      781 gtcagcaagc ggcagataaa tacagtgcca aaccgggtca aagcgaacac cagacaggtc
      841 ttgcaataga tattacatgt gaagccctaa attacaaatt ggatacttca tttgccgaaa
      901 cagaagaagg caagtgggtt tcagaaaacg ctcaccgttt cggatttatt atcagatatc
      961 ctaaggggaa ggaagaaatt acgggttatg catatgagcc atggcatata agatacttag
     1021 gggttgaact ggcagaagaa atatatgagt cgggactcac attggaagaa tattttcaat
     1081 aaaaagccac ggaatccccg tggttttatt tgatggaaag ttataaccca ttattatgaa
     1141 atccttcgct tatttctaag aaaaataaaa atatcaagca aacatttgca ggacacgata
     1201 ataattctta gcgaatgcag gttaaaaatc cgttaaacag cttgcgttgt ttgagcagag
     1261 cgagttcgca agctgtagga tttttagccg gaatgagctc ctagaattat tcgtggccga
     1321 aaccgtgagc ggatattttt atttttttta gtgtcctgag ccacatgatt ccatggcctt
     1381 tttatatcag ctcgtataca tcatttgcat attggacggg gtcttcgatt gtaagccctt
     1441 caataagcaa tgcttgatta aataatacgt ttgaaagctt tttaaatttt tctttatcat
     1501 tatgaaatgc atcttttaat ttttcaaaaa tttgatggct ggtgttaatt tcaagaattt
     1561 tctccgcatg gatgcttgaa ggtttatcag gcagtgcatt taacaccttt tccatttcta
     1621 tcgataattc tccttcggta gctaggcaca cgggatgagt tttaagtctt tttgaagctc
     1681 tcacctcttt aactttttca cccagtgatt ccttcatgaa agcaaacaac tctttatttt
     1741 cttcaacatt gtcggtattg tcatcttctg atttaatatc caggtcggag cttgatatat
     1801 tcttaaactc tttatctttg tagtttaata aaacttttat tgcaaattca tctatttcat
     1861 ctgtaaaata gagaatttca aatcccttgt cagcgatgag ttccgcctga ggaagttttg
     1921 ataatttatc aacactttct ccggctgcat aatatatgta tttttggtct tccttcattc
     1981 gactcacata ttcatcaagg gttgttagtt tttcttcata agatgaataa aacattacta
     2041 aatcctgaag aaattccttg tttaagcccc aatcgctgta taagccgtat tttaaaactt
     2101 tcccaaaatt tataaagaat ttttcataag tttctctatc ttccttcagc atagacaaga
     2161 gttcgctttt tattttttcc ttgattttct ttgctataaa ttgaagctgt ttgtcatgct
     2221 gcaatacttc tcttgaaata ttcagagaaa aatcgggcga atcaaccaat ccctgcacaa
     2281 aaccgaaata atcgggcaat aaatcaccgc attttttcat aatcattaca ccgtttgaat
     2341 aaagctcaag gcctttttcg aattcctttg tatagaaatc ataaggtgct cttgctggaa
     2401 tatataatat tgcattgtag cttactactc cttcagcgct tacatgagta tatttcaaag
     2461 gtttttcata gccgaaatgt ttttccgtat agaagttatg ataatcttct tccttaagct
     2521 cgcttttatt tttcctccat atggggacca tgctgtttaa aatttcatct tcaaaataat
     2581 cttcatattc attttccgtt ccttctttta gctttctttt tttcatcatc atcttgatgg
     2641 gatatttaat gaaatcggaa tatttcttaa ccagtgattt tatgttgtaa gcatcaagga
     2701 actcatcata atcttcatct tcagtgctag gtttgatttt catgattata tccgttccaa
     2761 cactatcttt tttgcatggt gtgattgtat atccgtctgc tcccgttgac tcccatttat
     2821 atgcttcctc ggaatctgct cttttgctta taaccgttac cttttctgaa accataaatg
     2881 cagaataaaa cccgactccg aattgtccga ttatatccac atcttctttt atttcattat
     2941 tctctttaaa atcaaaagag ccgctttttg ctatgatacc aagatttgaa tcgagctctt
     3001 cctttgacat gccgattccc gtgtcagata cccttaaaat tctttcctct tcatttgggg
     3061 taattcttat gtaaaagtca tctttgttaa aatcaatatt attgtccctt aaagacatat
     3121 agtatctctt gtcaatcgca tcactcgcat ttgaaataag ctctctcaga aatatttcct
     3181 tgtgagtgta aatggaatta atcattaagt tcaaaagtcg ctttgattct gatttaaatt
     3241 gttttttagc cattttatct tcctttctta aaatgatcta accctatttt ttaatttttt
     3301 gtattacatt gtattagcac tcggagcagt cgagtgctaa ccatatttta tcaaaactac
     3361 atagattgtc aatgggctaa ataaaaaatg agatccttcg ctatgcccag gatgacatgc
     3421 ctgtcattct gagagccagc acgaagaatc tcatctactg acgtttatca gtttctgaca
     3481 caactgtttt ctttgtataa tctgcaaaca tcatatattt taactaaggc attccatgtt
     3541 ttaatatcga ctattccgct tggggggagc atactccttt gctgaaaaag tcttactgct
     3601 tcttcggttt cttcatcaaa tacgtttgta acttctaatt tattataatt ggcatatctt
     3661 ctgcaaaatc cgttaatcat aatttttatc acatatacta tatctctttg gtcccctagt
     3721 ttcacgtcaa caaagtcacg ggtgctgaac gaaattttgt agggcgtttg agtttttttt
     3781 acacattcgt ggttttcatg tactaatgca ttcaatgttg gaacatccac gaaaccggta
     3841 ggagagagtc ccttgatttt ttgaaaaata aggactgcat tttgagttgc ttcatcatat
     3901 attcccgttg gggtaacaga aggcagtgaa ggatatagat tttttatgtt attaagatgg
     3961 ttttgaatta ccatcaccag atttttgtta aagcttaaat cagtaaccat aatatctcct
     4021 ttttatgtat tagtttctct ttcaagaaga gacaactgat atacttcgtt taatttattc
     4081 catgtttctt cattaacaat gccggtaaca ggcagcccat ataaattttg gaagataata
     4141 actgcagcag ttgtgtcagg accatatatg ccatcaatct caacaggtct tatttcggga
     4201 tgtatggcag aaattgcatt cagcataatt tgaagaagca gaattctcgg atattcaagc
     4261 cccatacctt cggttaattc cattccaatg aaaggaagaa cgggcagttc taattcctct
     4321 acaggagttt cagtaaggat taaatatgcc tcccgaaaca gcaaattcaa tgtttgttgg
     4381 tctactattc cggtaggatt caaccccata ataatttgaa attgtcttac tgcttcatca
     4441 gtatcaggac cgtagaacgt gtctatttca acaggctcta ttgcatcgta tttttgggat
     4501 aaaacgttta aaaaaaattc tatatatacc actacaggta aagtggctcc ctgcaataca
     4561 atatttgcat agagttcttg aagttcttca taggtaagtc ctgcggatac taactcggct
     4621 atgtttgtaa cagcaatata aataaagcgt attcggtacc atgtatctct atccacaatt
     4681 ccggtaacag gcaaattgaa tgcttcttga aaaattctta cggcattttc cgtttcttcg
     4741 tcaaaaaatc ctgttattct tacagccgga attaaaggga aattgttgtg tataacattt
     4801 agcgaatgct gcattctaaa aacatctatt cctgaatctc ctattgagag aggctcaccc
     4861 ggataggttc ctgtaatctc tactgcggca gtgctttcaa ctaatgttat gtcttcgcca
     4921 tagtagtatt taagaatttc ttcagctgta tatccttgat ttgccaattc aacacttccc
     4981 cattgataca tgccgtcgca ctgagtgatt cttccgtcgc agaaggctgc aaataatggt
     5041 tcgatatgac cttctctcgt aatatacaca ttaaagattt catttgcaat gttggaaata
     5101 gtgtcaaaaa tacctcgctc atgaacataa gcctggtcgt actgagttgt atttgttata
     5161 tcaaaatcat agcctcttga cctgtaccat tccgtgaata tcctattcat agcaaaagaa
     5221 gtaatagcat gaatatttgc ccttaaagct tcttccggcc atgtgggata taattcactg
     5281 gaggctacat tttttatata ttccaaatac ggtactgtta cattaagagc ttcttcatca
     5341 tcgggagctc ctaaatgcac agttatataa agaggaaaag gcacaatgac atgcgggagg
     5401 ggtaactcag ggataacaac atctatttca ggaatactca tactatcacc ttgctttcaa
     5461 ctattttaac cgccattcgt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nattgtgcct gttagaggaa gcttcatggt tttttgaaat tcaattattg cgttccttgt
     5641 ttcaggacct aagataccgt caatatcaac agcaggaatt gaatcataat aagctgacaa
     5701 aacatttaaa aagtattgaa ccaattgaac cctgggaaca acctgaactc cttcttctat
     5761 ctcagtaata tcttcgacta catcaccgac taagacccct cgggttgaaa tttctgcaag
     5821 ccttactata gttgcatatt ttgagccgat ttcaacccat gtagccaaat cgatgatacc
     5881 ggttaccggg agattaaata tattttgaaa ttctttcact gcagcttcca tattctcatc
     5941 aaatagagaa tttacagggg atattctggg tatggcaggg taatttacag ctatagcatt
     6001 taaggccatc tttgcaataa gtacatgttg gttgtcatca ccgagcctca gcggcgattc
     6061 cggatatgtt tgctgaatca ttgttacact catttataca cctcacacat ttgatctatc
     6121 aattgggctt gacggtatca ctaaggttcg ttcttcttca tcctcatcac cattttgact
     6181 tatcggtatg agagttacat taaaatttgt cgacgtgtcg gggaaaactc ttatgtcaag
     6241 aatattttgg gtataataac caattgcctg tattcttaaa ttatatgtag aaaaaaaata
     6301 ttcgcggctt tctgatgggt cttgagggtt gtatacaacc gggagagcca ttctcggaac
     6361 ttttccgctt atatcggtaa cgagatgata cagtgcttct tgatttcctt cttggtctgt
     6421 tacatacaat gtaacaacag catcttctac cggcaaggca cctaaggctg tgtttacgga
     6481 tacatttacg taacctgttt gtatagcggc gtcttcatta agctgatttt cgtttaataa
     6541 tggaagttca ggaggtgctt cagtttttct tattttattt ctggccacat acggtatatt
     6601 tcggcctctt ctattcataa aatcacacct ttctattgca taacggtaat attggtacat
     6661 ggtaattaat aagaatttgc ttacttaata tatatgtaca caactaaact attatgaggt
     6721 gatgatagaa ggtttgagca tctgccatac ccgtaaccgg cagaccatac aaactttgga
     6781 aggcaataac tgcagctctt gtgtctgaat caaatatgcc gcctatttca acaggcttta
     6841 tttcgggatg cattgcagaa atgctgttta acattatttc aagaaggagt attctcgggt
     6901 attccggtcc cataccctcg gataaatcta ctcccaaaaa gggaagcaag ggaagtctta
     6961 tatcttcgaa acgtactaat aatatcacct tgctttcata taaataaaac ttttcctcaa
     7021 attaagaaaa tcttatatcc atttaaatat atgtttataa cataaattta ttaaattttc
     7081 ttaactcttc tgaataaaaa gaagtgttct attttgtaaa aatttgtggt aaaatgtaaa
     7141 aacactcatt tcattttgct aaaatacaac acaataatat gttgttatat attaaaatgg
     7201 gtataggaat tgtcacttga atagcttgtc agattagaaa tttattaata gggtaaacaa
     7261 aaacaagggg agaaatatga taagcaatga ggacttaatc aggatgcacc tgattaacgg
     7321 gttaggcaga aaaactatct gcaaaatttt agcatgcatt gaatcagaaa atataaagct
     7381 taataatttt tcggaagttt tgaaactgtt aagcgatatg aaaatcaaaa gattaaaaat
     7441 tgatgaagaa aaaattgaaa aagaagcatc aataatattt gaatgctgca aaaaaaataa
     7501 aataaaaatt gttggtttat atgatgaaat atatccctac aaatttagat taatagaaga
     7561 taaacctttg ttgttgtttg ttgacggaga cttggaactt ttaaatcagg caaacaacat
     7621 agctatagta ggcacaagaa ctccatctca ggaaggatat gaaataagct cgatattagc
     7681 tgaaaaatta actgaagaag gttgttgtat cattagcggt tttgcatcag gatgtgatga
     7741 agcagctcac tacggaagcc ttcaggcaaa tggaaggact attgcagtaa taccttcagg
     7801 ccatatcaat gttttaaaac aaaacaggtc cttgtataat atgattattt taaacaaagg
     7861 ttgtattata tctgaactgc ctccgcttgc aaaggctgac aacaattctt tcatggacag
     7921 aaacaggctg atagcagctg tttcagaggg cctgatagta atagaagggg gcttgagggg
     7981 cggtacatca catgtggtta aatttgcaaa agaatacaac aagccggttg catatactgc
     8041 aaatatgtgc aaaataacgg gacaaacctt gatttttaaa gatatagata taatagacag
     8101 ctttgaaaaa ctggtaaaat ttaaaagcaa atcatgcaat aaaatccttg acaaagcaat
     8161 tcctcagtag tataatcagt tttgctgctc aacagttaag cggatgtggc ggaattggca
     8221 gacgcactag acttaggatc tagcgcagca gcgtgggggt tcgagtccct tcatccgcac
     8281 caaaacaata gctccgatat gaacggagct tttattttgc acatatagat taatttttga
     8341 aaaagataag ataat
//
LOCUS       DAOSHP010000072         8277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7387_length_8277_cov_13.745439, whole genome shotgun sequence.
ACCESSION   DAOSHP010000072 DAOSHP010000000
VERSION     DAOSHP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_8277_cov_13.745439"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..393)
                     /locus_tag="PLB33_07290"
     CDS             complement(67..393)
                     /locus_tag="PLB33_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG63365.1"
                     /translation="MMNGTNKKISHDTRELLHKWVWPDNVRELENAIEYAYYAAEEDE
                     ILPGHLQLKFTKFSDKHTPTLREAEAEAVKKALNQSKNNVTQAAKILGIGRNTLYKKI
                     KEYNIV"
     gene            705..>1243
                     /locus_tag="PLB33_07295"
     CDS             705..>1243
                     /locus_tag="PLB33_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63366.1"
                     /translation="MYKKILILLFVCAMLSSCKKDENVLPVVETPVEYNVEDKYELTA
                     LNSDKQGIDNTTVFQLTSKVELNDSFIKNNLEILPAEDYKIEKVSGTLHNIIPLADLR
                     NDQIYQIKINDKDEDYNYSWAFQTKKKFEVEGTLPADKSSYVPSNSGIEMYFSLSGLG
                     ELDDFFEIKPHVEGKFLYK"
     assembly_gap    1244..1343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1344..5333
                     /locus_tag="PLB33_07300"
     CDS             <1344..5333
                     /locus_tag="PLB33_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOG63367.1"
                     /translation="DIEGLTFLNALKQKPNIVNKYNNPKGLFELPDLPKGYYLLEFST
                     DDGETNYHFLQINNMLIYYALFSNQIVVTASDGADSKGIVDAAVVLNGEHMGFTDNNG
                     MLIIDRDTSVYENKAISLRVEAKGFNDFVYADSLYFYNYYYNSQYEAYKYLRYIDTDR
                     PVYLPTDTINVWGFARHKDDKSVNKVRIELVEVHTNLVFEEKTVDLTNIGTYQTEFEI
                     KNAASEYFNFQINVYDNDLRISTKYISIGKYTKPLFTISGEFDKKFVYSGESINYKVN
                     ANFFDGNPLPGLELKFGGFPTNYSGSIDYGQMDSTLKLNEKGETLISVNTNVNSDSWR
                     PVSIRTTLWNAKAEDKSVGVMDTFEIFPHHKMVEIEQNDMDNPENIRILLHELDITKY
                     DSQYFSGYEKLRGNPLSGTVLVSVTESYYEKYKKGEHYDYINKVNVIEYGYNHIKNIV
                     FNEITEITGGCGNVIIPDFNPQRTYEVAVEYMDENGVIREYSYVGSYYRFYERPYYYL
                     EKDDDNKQNYRLNDNINLNLKYSEENVESTDKDKLILLIMRDGLIDYKITDDTKLDFE
                     FKEEYIPNVMINSIYFKNGYMYPVEFINSLYYDTTERQIHFDVTTDKENYRPGEEVLL
                     SIKTKDENNKPIKADVNISVVDEAYFALFEKRVDTLNSLYGGGWSTGLNASYLSNIDL
                     SIESGNGAERGGGGDNFTGFRDEFKDTNIFKTITTDKNGNGELKFKLADNLTSWRITY
                     QGISDKKFAGSGTKNITVSLPFFVDIIMSEEYLKDDKISASLRVFGTEAKESEEVNYE
                     IKITNKETGKELKYAQTGIIGDYTNISFDKLSEGTYEISVYAAYKDFEDGIKEEFNVV
                     DSFVYFNNTTYYKLSEDTVLSKVYSNPVITLFNESRSDFYNSLESILGNYGKRIDQTV
                     CSMLASKYINDYFKTDLTFNEDDLISEINRYEIQTSGGYSLFTYSNTDAEITAKLMNL
                     VDNEYLRFKSKTYFKNILGNPDYNSKIPAALWGLSTFKEPVLLTVYDLLENNTLEIRD
                     KIYLSLALAEFGDFNTAKKYYKEFSDSFKESGDYLYFESGADENYKYSENYELTALLS
                     VLGCKLQDYDTSDKLFKYIYNNPSKFTLSNFEQLIYIMDRNIMNLDEIKDLFGEVTVT
                     SEGKDKTYKLNLFDRENFSVPKDKIQDIKFKNIKGSIACKVEALGNKDDLDKNKTEDF
                     SINANYTLKDSTESQMSYNHSDLVKVTIMPSFSDNVVEGNYEITYVVPAGFRFIELDR
                     KWPFWVEENGQKLRLFFYYNRQTVAAIPITFYIQAAQIGEYTVDYVVIKENLETRLNY
                     IDKEVLTVQ"
     gene            5425..6318
                     /gene="amrB"
                     /locus_tag="PLB33_07305"
     CDS             5425..6318
                     /gene="amrB"
                     /locus_tag="PLB33_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOG63368.1"
                     /translation="MSRILKITVLFLLFIAVTGSIFAYNKTVNNKEPEPYTYKGGKNI
                     ECLYYNATDFINIPYYETIKVGGDILGCIVPHHLAAKDLIHEVFQNTSKDKYKTVVLI
                     GPDHESIDKGKIFTTLSNWQTPTGILETGREITEELLKCSFVKEDADKLTIEHSASSI
                     IPFINYYLKDVKVVTLVFTKQVKIEDVETLTDILYENVDLEETLFIASVDFSHYLNLQ
                     NADKMDLVSMEAIKNKDIDKIMSFTNDNLDSPISIVTMLKLMDKAGSSENTVLNHSNS
                     ELELKKKIEETTSYITYLFYR"
     gene            6468..7127
                     /locus_tag="PLB33_07310"
     CDS             6468..7127
                     /locus_tag="PLB33_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63369.1"
                     /translation="MENYLKKLIRLIFGLFIYALGLVLCIHADIGLAPWDAFGIGINK
                     VTGISYGNVSIITGIIILIALVMIFKEKIGFATILNTILIGVFADLIINSKLIPYMQN
                     FFTGVLMLITGQIVICIASYLYIGIGLGAGPRDSLMVALGKKFPNVPIGAVRGAIEGT
                     VLLIGWLLGAKVGLGTVIYVFSISFVLQTTFRLLKFDVKAVVHESVFDTINMFKEMRA
                     S"
     gene            7142..7741
                     /locus_tag="PLB33_07315"
     CDS             7142..7741
                     /locus_tag="PLB33_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOG63370.1"
                     /translation="MCGRYYIDIDNNEIKKILEEAQRNIYENFKTGEIFPTNIAPIYI
                     EDDNKIKPALAKWGFPKWDGKGVIINARAESINERQMFKRLVLSSRCIVPASYYFEWK
                     KEEHKKDKYKITNPGSNIYMAGLYNIITDKNKQLSLFDESMDIYYTIITRSANDSVSR
                     VHSRMPLVFDVEEMNEWLKGKNIDELLKADHELFSEKTK"
     gene            complement(7764..8093)
                     /locus_tag="PLB33_07320"
     CDS             complement(7764..8093)
                     /locus_tag="PLB33_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOG63371.1"
                     /translation="MEIITSYEKLQELIEKNDMVLVYFGIDDCGVCKSMKPKVENMIK
                     RYPNIHAVIVKADKSPSISARFNVFTVPVILLYIEGKETIREVRIISLDNLEQKIARY
                     CQIFYVQ"
BASE COUNT         2927 a         1204 c         1484 g         2562 t
ORIGIN      
        1 ccctgatggc ggctgtatag cccccgggac cgccgcctat gattgctatc ttcatgaata
       61 cctccttcat acaatattgt attcttttat ttttttatat aaggtatttc gtcctatacc
      121 taatattttt gcagcttgag ttacgttgtt ttttgactga ttcaatgctt tttttacagc
      181 ttcagcttca gcttctctta gtgttggtgt atgtttatct gaaaatttgg tgaatttcag
      241 ctgtaaatgc ccaggcagga tttcatcttc ttctgcagca tagtaagcat attctattgc
      301 attttccaat tctcttacat tgtccggcca aacccattta tgaagcagct ccctggtatc
      361 atgtgatatt tttttattgg ttccattcat catattgtac tgatttaata cgtagtcaga
      421 aataatatcc agatcatcct gcctgtctct taaagtacag ctttaataat acaattatat
      481 cataagaatt tcaaatcaga agcggcattg tttaaatctt ttgaactttt ttaatcattg
      541 tatcgtcaaa tattaaatgg tataattgac ataaggcatc gaatcgcaat aatgaatacg
      601 aaaacttaca attcggcaaa ttgaataatc ttatgttttg tgaattccta aatctgttgg
      661 aaacggcata agctttgatt attactaaag aaaggcagga aattatgtat aaaaagatat
      721 tgatactttt atttgtttgt gccatgcttt cttcctgtaa gaaggatgaa aatgtattac
      781 ctgtagtaga aactccggtt gaatacaatg ttgaggataa gtatgaactt actgccctaa
      841 actcggataa acagggaatt gacaatacta ctgtttttca attgacttca aaagtagaat
      901 taaacgatag ctttattaaa aataatttag agatacttcc ggctgaagat tataaaattg
      961 aaaaagtatc aggaactcta cataatatta ttccccttgc agaccttcga aacgaccaaa
     1021 tctaccaaat caagataaat gataaagatg aagactacaa ttattcctgg gcttttcaaa
     1081 caaagaaaaa gtttgaagta gaaggtacgc tgcctgcaga taaaagcagc tatgtaccgt
     1141 caaattcagg aattgaaatg tatttttcct tgagcggctt aggagaattg gatgattttt
     1201 ttgaaattaa gcctcatgtt gaaggaaaat ttctgtataa aaannnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnngatattg aagggttaac ctttttaaat gctttaaaac
     1381 aaaagcctaa cattgtgaat aaatacaata atccaaaagg gttgtttgag ctgccggacc
     1441 ttccgaaggg atattattta ctggaattct caacagatga cggcgaaaca aattatcact
     1501 tcctgcagat taacaatatg cttatttact atgctttgtt ttcaaatcaa atagtggtta
     1561 ctgcatcgga tggtgcagac agcaagggga ttgtagatgc ggcagttgta ttgaacgggg
     1621 agcatatggg ctttacagac aataatggaa tgctgattat tgatagggat acgtctgttt
     1681 acgaaaataa ggcaatttcc ctaagagttg aagcaaaagg tttcaacgat tttgtgtatg
     1741 cagacagcct ttacttttac aactattatt acaacagtca atatgaagct tacaaatatt
     1801 taagatatat cgacacggac agacctgtat atcttcctac agatacaatt aatgtatggg
     1861 gatttgcaag gcataaggat gataaatctg ttaataaggt tagaattgaa ttagttgaag
     1921 tccacacaaa tttagtgttt gaggaaaaaa ctgtagacct tacaaatata ggcacctatc
     1981 aaacagagtt tgaaataaaa aatgctgcat cagaatactt taactttcaa ataaatgtat
     2041 acgacaatga tttaagaata tctacaaaat atatttcaat cggcaaatat acgaaacctc
     2101 tttttacaat aagtggcgaa tttgacaaaa aatttgttta cagcggtgaa agtattaatt
     2161 ataaggttaa tgccaatttc tttgacggca atcccctgcc cggattagag ctaaaattcg
     2221 gcggctttcc aaccaactac agcggaagca tagactacgg acaaatggat tcaactctta
     2281 agttaaatga aaagggagaa actctaataa gtgtaaatac aaatgtaaac agtgacagct
     2341 ggagacctgt ttctataaga acaacccttt ggaatgccaa agcagaggat aagtctgtgg
     2401 gggttatgga tacatttgaa attttccctc accataagat ggttgaaatt gagcaaaatg
     2461 atatggataa tcctgaaaat atccggattt tgctccatga attggacatt accaagtatg
     2521 acagccaata cttcagcggt tatgaaaaat tgaggggcaa tccattgagc ggcacagtgc
     2581 tcgtaagtgt gacagaaagt tattatgaaa agtataaaaa aggtgagcat tatgattata
     2641 taaataaggt aaatgtcatt gaatatggat ataaccatat taaaaatatt gttttcaatg
     2701 aaattacaga aataaccggc ggctgcggca atgttataat tcctgatttc aatcctcaaa
     2761 gaacttatga ggttgcggta gaatatatgg atgaaaacgg agttataaga gaatactcct
     2821 atgtaggaag ttattaccgc ttttatgaaa ggccttatta ttatttagaa aaggatgatg
     2881 ataataaaca aaattacagg ctaaatgaca atataaattt aaatcttaaa tacagcgaag
     2941 aaaatgttga aagcacagat aaggataaac ttatactctt aataatgaga gatggtctca
     3001 ttgattataa aattactgac gatacaaaac ttgactttga atttaaagaa gaatatatac
     3061 ctaatgttat gattaactct atatatttta aaaacgggta tatgtatccg gtggaattta
     3121 taaactcatt gtactatgat accacagaaa gacagattca ctttgatgta actacagaca
     3181 aggaaaatta cagacccggc gaagaagttc tattaagtat taaaacaaag gatgaaaaca
     3241 ataagccgat taaggcagat gttaatattt cagttgtaga tgaggcatat tttgccctct
     3301 ttgaaaaaag agttgatact ttaaattcat tgtatggcgg tggttggagt acaggcctta
     3361 atgcatctta tctgtcaaat attgatttga gcatagaatc gggaaacggt gccgaaagag
     3421 gcggcggtgg agataatttt acaggtttca gagatgaatt caaagataca aatatattta
     3481 aaacaataac aaccgataag aacggcaatg gagaattaaa atttaaatta gctgataact
     3541 taacatcctg gagaataacg tatcaaggaa tttcagataa aaagtttgca ggaagcggca
     3601 caaaaaatat tactgtaagt ttgccctttt tcgtagatat aataatgagt gaagaatacc
     3661 ttaaagatga taaaatatca gcttccttaa gggtatttgg aacggaagca aaagaatctg
     3721 aagaagtaaa ttatgaaatt aaaattacaa acaaagaaac aggtaaagag cttaaatacg
     3781 cccaaacagg tataatagga gattacacaa atatttcatt tgataaatta agtgaaggca
     3841 cctatgaaat ttccgtttat gctgcatata aggattttga agatggtata aaggaagaat
     3901 tcaatgtggt tgattcattc gtttatttta acaataccac ttattataaa ctgtcagaag
     3961 atacagtctt aagtaaagtg tattcaaatc ctgtaattac attgtttaat gaaagccgct
     4021 ctgactttta caacagtttg gaaagtattt taggcaacta cggtaaaaga attgaccaga
     4081 cagtatgctc gatgctggca tcaaagtata tcaatgatta ttttaaaaca gatttgactt
     4141 tcaacgagga tgatttgatt agtgaaataa acagatatga aatccaaaca agcggcggat
     4201 acagcctctt cacatattca aatactgatg cagagataac cgccaaattg atgaacttag
     4261 ttgataatga atacttaaga tttaagtcta agacatactt taaaaatata ttgggaaatc
     4321 ccgattacaa ttcaaaaatc ccggcagctc tttggggatt aagcaccttt aaggagccgg
     4381 tattattgac ggtttatgat ttattggaaa acaacacatt ggaaatcagg gataaaattt
     4441 atctatcttt agctttagcg gagtttggag actttaatac agctaagaaa tattacaaag
     4501 aattttcaga cagctttaaa gaatcaggag attatttgta ttttgaaagc ggagccgatg
     4561 aaaattataa gtattctgaa aactatgaat taacagcact cttgtccgtt ttaggatgca
     4621 aacttcaaga ttatgataca agcgacaaat tatttaaata tatttataat aatccttcta
     4681 agttcactct ttcaaatttt gagcagctta tatatattat ggacaggaat attatgaact
     4741 tggacgaaat aaaagacttg ttcggcgaag tgactgttac atcggaaggt aaagataaaa
     4801 cttataaatt gaatttattt gacagagaaa acttttctgt tccaaaggat aaaattcaag
     4861 acattaagtt taaaaatatt aaaggcagta tagcctgcaa ggttgaagca ttggggaaca
     4921 aagatgattt agataagaat aagaccgagg atttttcaat caatgcaaat tatacgctta
     4981 aagattctac agaaagtcaa atgtcataca atcattctga cctggttaaa gtaaccataa
     5041 tgccatcatt cagcgacaat gttgtagaag gtaattatga aataacatat gttgttccgg
     5101 caggtttcag atttattgag cttgacagga agtggccatt ttgggttgaa gaaaacggtc
     5161 agaaactaag acttttcttc tactataaca ggcagactgt cgctgcaatt cctattacat
     5221 tttatattca agctgcacaa ataggagaat atacggtgga ttatgtggtt ataaaagaaa
     5281 atctggaaac caggttaaat tatattgaca aagaagtttt aacggtacag taataactaa
     5341 ccaatatatt gggcaaaacc cttgtcattt ttggccgaat gtaaataatc tcacctttta
     5401 acaaagcaaa gtgaggtgta agtattgagc agaatactaa aaatcacagt tttatttctg
     5461 ttgtttatag cagtcacagg cagtatattt gcttataaca agacagtaaa taataaagaa
     5521 ccggagccgt atacttataa gggcggaaaa aatattgaat gcctctatta taatgcaaca
     5581 gactttatca acattcctta ctacgaaaca attaaagttg gtggtgatat actgggctgt
     5641 atagtacccc atcatttggc tgcaaaggac ttaattcatg aggtttttca aaatacgagc
     5701 aaggataaat ataaaaccgt agtattgatt ggtcccgacc acgaaagcat agataaaggc
     5761 aaaatattta caaccttaag caattggcag acacccacag gcatattaga aacaggcaga
     5821 gaaataacag aggaattatt gaaatgcagc tttgtaaaag aagatgctga taaattaaca
     5881 attgagcatt ctgcctcttc aattattcca tttataaatt attatttgaa agatgttaaa
     5941 gtagtaacct tggtttttac aaaacaggtg aaaatagaag atgtggaaac attgacagat
     6001 atattgtatg agaacgtaga tttggaggaa acattattca tagcttcagt cgacttttca
     6061 cactatttaa atttacagaa tgccgataaa atggatttgg taagcatgga agcaataaaa
     6121 aataaagata tcgataaaat tatgtctttt accaatgata atttagattc acccatatcc
     6181 atagttacaa tgcttaaact catggataaa gcgggctctt cagaaaatac cgttttaaat
     6241 cacagcaatt cagagcttga attaaaaaag aaaatagaag aaactacaag ctatataacc
     6301 tatttgtttt atcggtaggc atcgttttag gaaagggcgt taatgtcaat gcatgttacc
     6361 gttcgctcat ggaaaaataa gagccctatt ttaatacttt tttttattgt tcttgttatt
     6421 tttcatggta tcatataaaa ttagcttatt gaaagcagga ggatttaatg gagaattact
     6481 taaaaaaact tattcgatta atattcgggc tttttatata tgccctgggt ttggttttat
     6541 gtattcatgc tgacatagga cttgctccct gggatgcttt cggtatcggc attaataagg
     6601 ttaccggaat atcttacggc aatgtatcaa ttattacagg tattataatt ttaatagcac
     6661 tggttatgat atttaaagaa aaaataggtt ttgcaacaat attaaacaca atactgatag
     6721 gagtctttgc tgatttaatt ataaattcaa agctgattcc ctatatgcag aattttttta
     6781 ccggggtttt aatgctcata acagggcaga ttgtaatatg catagcatcc tatttgtata
     6841 taggaatagg tcttggggca ggaccaaggg attccttgat ggttgcttta ggaaagaaat
     6901 tcccgaatgt accgattgga gcagtcaggg gggcaataga gggtactgtt ttgttaatcg
     6961 gctggctttt gggagccaaa gtgggattag gaactgttat ttatgtattc agtataagtt
     7021 ttgtacttca gacaacattt aggctgctta aatttgatgt aaaagcagtt gttcatgaaa
     7081 gcgtatttga cacaataaat atgtttaaag aaatgcgggc ttcataaaca gtgaggtaga
     7141 tatgtgcgga agatattata ttgatataga taataatgaa attaaaaaaa tattggaaga
     7201 agcccaaaga aacatttatg agaattttaa aaccggtgaa atttttccta caaatattgc
     7261 tcctatttat atagaggatg ataataaaat caagccggcc ttggctaagt ggggattccc
     7321 caagtgggac ggcaaaggtg taataattaa tgcaagagct gaatcaataa atgaaagaca
     7381 gatgtttaaa agattggttt tatccagcag atgcattgtg cctgcttcct attattttga
     7441 atggaaaaag gaggaacata agaaggacaa atataaaata actaacccgg gctccaatat
     7501 ttacatggct ggattataca atataattac ggataaaaac aagcagttaa gcttgtttga
     7561 tgaaagcatg gatatttact atactattat aacccgcagt gccaatgatt ctgtttcccg
     7621 cgtacacagc agaatgccgc tggtttttga tgttgaagaa atgaatgaat ggctgaaagg
     7681 gaaaaacatc gatgaattat tgaaagcgga ccatgagctt ttcagcgaaa aaaccaaata
     7741 attgtaataa ttctattaag catctactga acataaaata tctggcaata ccttgcgatt
     7801 ttctgctcta aattgtccag gctgattatt cttacttccc tgattgtttc ttttccctca
     7861 atatataata atattacagg aactgtaaac acattaaatc ttgcagatat actcgggctt
     7921 ttatccgctt taacaattac agcgtgaata ttaggatatc ttttaatcat attttccact
     7981 ttaggcttca tggacttaca tacgccgcaa tcatcaatcc caaaataaac caataccatg
     8041 tcattttttt caataagttc ctgcagcttt tcataagatg ttataatttc cattgtagtc
     8101 tccatttatg tatttattta cattttacca caatattata aaataaccaa tcttttataa
     8161 atcaaaaaat atatcttgca ttttatattg tcttatgcta atattctaat atattaatat
     8221 actaatatga cgaggtgaaa atatctcatg cttctttagt tatgtatacc tttaaat
//
LOCUS       DAOSHP010000073         8058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7676_length_8058_cov_6.614769, whole genome shotgun sequence.
ACCESSION   DAOSHP010000073 DAOSHP010000000
VERSION     DAOSHP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7676_length_8058_cov_6.614769"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1117
                     /locus_tag="PLB33_07325"
     CDS             173..1117
                     /locus_tag="PLB33_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG63372.1"
                     /translation="MEILISLMAAVFIYSIFSILISSYGPGQVRNRISKYFTEYNADD
                     VHEQFIREKNEEESKRKKNRFKIASKEFSNYIASSGIRLKANEFIYFWAGLTLIPMIL
                     VLLFSGNIITGAALSIIGISIPPFIVSNATKKREDLFTKQLGDATVIMGNSIKGGFTF
                     LQSMENIAEEMQPPISTEFSKVLREIHFGVKQEDALSHMVERTKNKDLELLISAVMTS
                     AQVGSNLSEILDTISNTIRDRIRIKNEIKTLTAQGRMSGLVIGLLPIVLILAIMIMTP
                     SYFAGFFDSTIGKILISISVVLEITGFAIINKIVNIKV"
     gene            1125..2057
                     /locus_tag="PLB33_07330"
     CDS             1125..2057
                     /locus_tag="PLB33_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG63373.1"
                     /translation="MALYISGFFAIFIFSAIIIILSSFGLNHDKVNNRVSKLIDIESN
                     IIEDEELNKSLSERFISPIMNSIVKSLGKFLPDTGLNNQKSENLRKSLRQAGFTLKPK
                     EYSAIRIFIIWGNALLYFVLGLVLNVSYLYLFLLPLFGAYLAFTVMRFNLMFRITKRK
                     DAMERQLPDVLDMLSVNVEAGLGFEQALLHIIEHFEGALIDEFNITYREMSMGRSRRD
                     ALILLGERCDIDDLKSFTGSIIQANKLGISLKNVLHTQAASIRENRRSKIEEKAHKMS
                     IKILLPMVIFIFPVIFIILMGPAVVSIAEMFGGM"
     gene            2057..2389
                     /locus_tag="PLB33_07335"
     CDS             2057..2389
                     /locus_tag="PLB33_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOG63374.1"
                     /translation="MKLYVNNNLLSNNITIADSFKTKLIGLLNKKSLDKNEGLLLKNC
                     TGIHCFFMKFTIEAVYLSKDMKVLYKETVKPWEMGRLVRNCTHVLELPEGMAKDIEIG
                     DYVILEGL"
     gene            2389..3450
                     /locus_tag="PLB33_07340"
     CDS             2389..3450
                     /locus_tag="PLB33_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63375.1"
                     /translation="MESDNNIVGILRDKDEADLDGVLKTTFGGYTKQSVQEYLSLIHK
                     QQQTTKETFSKNLQNLFDEKEDLRKNNETLQARLNKLSSEYDNLAGSLKDIVLDDSEY
                     SAQNVLELKNKIVSLEEQIKVTNRENYSLERKTEQLNNEIDELKSKLALSIKEYEAQK
                     EMLKSERLELKRQRDVVADISNKLQEEKNEVKYLKGTISDGKFAELNSKIGQLSTQLS
                     AQTEVNSKLNEEIALKDKTIDSLNDEVSLLKEKTNNLMKTLQEYNIQNDKLLIANEAL
                     DYQLQEEYKKAIAFINEKSNITIDRLIAQKNLSVAEAKIAALEVQLLKTNSSIEVKKE
                     INRMDTGVSDKEQKENMSF"
     gene            3490..4701
                     /locus_tag="PLB33_07345"
     CDS             3490..4701
                     /locus_tag="PLB33_07345"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOG63376.1"
                     /translation="MFRKLYRNETGQTIVLVALMMVMLIGFGALTIDVGAMTFQKSNL
                     QNAADSAALAGVQNMTSVSDVKKAVINYARSNGLKVTEDGAPQSGDTITINTPYNGDS
                     KKVEVIITREVKHHLAGVLGLTQSNISARAVAEKSGFLGAAFNYAVFHGSEDDPLRFN
                     SSSIDVDGDFHSNHAIQFNSLYANIKGTLEAASLLLINSATIDIRDVCLVSSITKNSS
                     TLNVNGSTILNPAPIVEMPDFSDILKEEAQTGITYTGNKTFNSGNFSFNQPIYVTGDL
                     TLNSGNFYFDCPIYVEGKLTINSTNFIGKGIIYAKNDIKVNSASFATTAGTVSLYSGK
                     NIELNSMWADINGFMYAPNGTIIMNSMSSQVNGSVIGNEIKLNSGYLKVNYGGGNYDG
                     LPDGGSVKLIE"
     gene            4800..5183
                     /locus_tag="PLB33_07350"
     CDS             4800..5183
                     /locus_tag="PLB33_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HOG63377.1"
                     /translation="MKSLIRLRMSTQDAHYGGNLVDGARMLGLFGDVATELLIRNDGD
                     EGLFVAYDNVEFLAPVYAGEFIEAEGEIVSKGNSSRKMVFEARKVIVPRTDISDSACD
                     VLEEPVVVCRASGTCVVPKGKQRQK"
     gene            5511..7190
                     /locus_tag="PLB33_07355"
     CDS             5511..7190
                     /locus_tag="PLB33_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOG63378.1"
                     /translation="MQKSNWKLGELLLYAGKISKEQLNEALEDQKINKKKLGEIIVDK
                     AWLKFEEIVETLELQLGFPKVDLSKYEINSNVATLIPESIVKKYKLIAIDIKDDMLLV
                     AMSDPLNFFAIDDIKLYTKMGVIPCLSSVPDVERAIERYYSSILTQKALNEFSDAFIP
                     AEDDTDSEEEMLEVATAPIVKLINSIIEQAMRGNASDIHIEPSHNDIRVRFRIDGDLK
                     EIMVLPKSNLSAITTRVKIIGRMDIAEKRIPQDGRVEMKFNGNEVDLRISTLPTVYGE
                     KIVIRILDKSNFNFTKEGLGFSKDNLDKLNKMIAQPYGMLLVTGPTGSGKTTTLYTVL
                     RELNVQTKNVITVEDPVEYKLKGINQTQVNVKAGLTFAAGLRSILRQDPDTIMIGEIR
                     DSETAEIAVRAAITGHLVLSTLHTNDSPSTIARLIDMGLEPYLVSSAVIGIISQRLVK
                     LLCPKCKEKYKASDLERKILGIDGDKETTLFKPVGCNSCNNGYRGRAAVHEVMLVNEN
                     IRRLINTGANTDDIRSQALKDGMSTLLQSAADLALKGDTSFEEIMRAGYTL"
     gene            7210..8004
                     /locus_tag="PLB33_07360"
                     /pseudo
     CDS             7210..8004
                     /locus_tag="PLB33_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
BASE COUNT         3030 a         1176 c         1637 g         2215 t
ORIGIN      
        1 gggataccat cacattgcag gatatattta catacaaggt tgagggtatt gacagcaacg
       61 gaagaatgaa aggaaacttt tcatcaacag gcatacggcc taactttctt gataagctga
      121 catcaagcgg tataatcgtc agagatgact ggtttgtaaa ttgaggtgaa atatggaaat
      181 attaatatct cttatggctg cagtatttat ttattcaatt ttttctattt tgatatcctc
      241 atatggaccg ggacaggtca gaaacaggat ttcaaaatat tttacggagt ataatgccga
      301 tgacgttcat gagcaattca tcagggaaaa aaatgaagaa gaaagcaaaa gaaaaaagaa
      361 tagatttaaa attgcaagca aggaattttc aaattatata gcttcttcgg ggataaggtt
      421 gaaagcaaat gaattcatat atttttgggc aggcttaact ttaattccga tgattttggt
      481 tttgcttttc agcgggaata taattacagg agctgcctta tctattatag gtatttccat
      541 accgccgttt atagtcagta atgcaacaaa aaagcgtgag gacttgttta ctaagcaatt
      601 aggtgatgcc actgtaataa tgggaaacag cattaaaggc ggttttacat ttttgcagtc
      661 tatggaaaat attgcagagg aaatgcagcc gccaatatct actgaatttt caaaggttct
      721 tagagaaata cattttggcg tcaagcagga agatgccctc agtcacatgg tggaaaggac
      781 aaaaaataaa gatttggagc ttttgatatc cgctgtaatg acttcagcac aagtaggaag
      841 caatttatcg gaaattcttg ataccatttc aaatacaatc agagaccgca ttagaataaa
      901 aaatgaaatc aaaacattga cagcccaagg acgcatgtcc ggattagtta taggattgct
      961 tccaatagtt ctgattttag caataatgat tatgactcca agttattttg ccggtttttt
     1021 tgattctaca ataggcaaaa ttttaatctc aatatctgtt gtattagaaa ttacagggtt
     1081 tgcaattata aataaaatag taaatataaa agtgtgaggt gcatatggct ctttatattt
     1141 ccggattttt tgcaattttc atatttagtg caattattat tatattaagc agcttcggtt
     1201 tgaaccacga caaggttaat aacagggttt ctaaattaat tgatattgaa agtaatatca
     1261 ttgaggacga agagctgaat aaatcattgt cagaaaggtt catcagccct attatgaatt
     1321 ctattgttaa atccctaggc aagtttctgc ctgatacagg ccttaataat caaaaatcag
     1381 aaaatttacg aaaatcacta agacaggcag ggttcacttt aaagccaaag gaatattctg
     1441 ctataagaat atttattata tggggaaatg cacttttata ttttgtactt ggcttagtat
     1501 taaatgtcag ctatttatat ttatttttat taccgttatt tggagcatat ttagctttta
     1561 ccgtaatgcg gtttaactta atgttccgca taactaaaag aaaagatgca atggagagac
     1621 agcttcccga tgttttagat atgctaagcg ttaatgtaga ggcaggatta gggtttgaac
     1681 aagcactgct gcatattata gagcattttg agggagcgtt gattgatgaa tttaacatta
     1741 cttacagaga aatgtccatg ggcagaagca gaagagatgc actgatacta ttgggcgagc
     1801 gctgtgatat tgatgactta aaatccttta ccggctctat aattcaagcc aacaaattag
     1861 gtatatctct taaaaatgtg ctgcacacac aggcagcctc catcagagaa aacaggcgca
     1921 gcaaaataga agaaaaggca cacaaaatgt ccattaaaat tctattgccg atggttatat
     1981 ttattttccc tgttattttt ataatattaa tgggaccggc tgttgtgtcc atagcggaaa
     2041 tgtttggtgg gatgtaatga aactatatgt taataataac ctgctgtcaa ataatattac
     2101 aatagctgac agttttaaaa ccaaactgat tggactatta aataagaaaa gcttggataa
     2161 aaatgaaggg ctgcttttaa aaaactgtac cgggatacat tgctttttca tgaaattcac
     2221 cattgaagca gtctacctat ctaaagatat gaaagtatta tataaagaaa ctgtcaagcc
     2281 atgggaaatg gggaggcttg ttagaaactg cacccatgtt ttggagcttc ccgagggtat
     2341 ggcaaaagat attgaaattg gagattatgt aattttggag ggattataat ggaaagcgat
     2401 aataatatag taggaatatt gcgtgataaa gatgaagcgg atttggacgg tgttttaaaa
     2461 acaacctttg ggggctatac aaagcaatca gtgcaggaat acttatcatt aatccacaag
     2521 cagcagcaaa caacaaagga aacattttca aaaaacctcc aaaatttatt tgatgaaaag
     2581 gaagacttaa gaaaaaacaa tgaaacactt caagcacggc taaataaatt atcttcggaa
     2641 tacgacaatt tagccggctc attaaaggat atcgttcttg atgattctga atattcagct
     2701 caaaatgttt tggaattaaa aaacaaaatt gtatccttgg aagaacaaat aaaggtaaca
     2761 aatcgtgaaa actattcctt ggaaagaaaa actgagcaat taaacaatga aatagacgag
     2821 ttaaaatcca agctggcact ttcaataaag gaatatgaag ctcagaaaga aatgcttaag
     2881 tctgaaagac ttgaactgaa aagacaacga gatgttgtgg cagacatttc gaacaaattg
     2941 caggaagaaa aaaacgaagt aaaatattta aaaggaacaa tttctgacgg gaagtttgca
     3001 gaattaaatt caaaaattgg acaattaagc actcagttgt cagcacaaac agaagttaac
     3061 agcaaattaa acgaagaaat tgctttaaaa gataaaacca ttgattcgct taatgatgaa
     3121 gtttctctgc ttaaagagaa aacaaacaat ttaatgaaaa cattacagga atacaatatc
     3181 caaaatgata aattactgat tgcaaatgaa gctttagatt atcaattgca ggaagaatac
     3241 aaaaaagcaa ttgcattcat taatgagaaa tctaatatta caatagacag attaattgca
     3301 cagaaaaatt taagtgtggc agaagcaaaa attgctgcat tggaagtaca gcttcttaaa
     3361 acaaacagct caattgaggt aaaaaaagaa ataaatagga tggatacagg tgtttcagac
     3421 aaagagcaaa aagagaatat gtcattttaa ctgagagaga agctaatttt aataaagggg
     3481 gaaaataata tgtttaggaa actttacaga aatgaaaccg ggcaaacaat agttttagtt
     3541 gctttaatga tggtgatgct tataggattc ggtgcattga cgatagatgt aggagcgatg
     3601 acatttcaaa aaagcaactt gcagaatgcg gcagattcgg cggcattggc aggagtacag
     3661 aatatgacaa gcgtaagcga tgtcaaaaaa gcagttataa attatgccag aagcaatggg
     3721 ttaaaagtaa cagaggatgg agcacctcaa tcaggagaca caattacaat aaacactcca
     3781 tataatgggg attcaaaaaa agttgaagtt attataacca gagaagttaa gcatcattta
     3841 gcaggtgttt tgggattaac gcaatcaaat atatctgccc gagctgttgc cgagaaatcc
     3901 ggttttttag gtgcagcgtt taattatgcg gttttccatg gctccgaaga tgacccgttg
     3961 agatttaaca gcagcagtat tgatgttgat ggcgattttc actctaatca tgcgattcaa
     4021 tttaacagtt tgtatgctaa tattaaggga acactcgaag ctgcatcctt attattgata
     4081 aacagtgcta caatagatat tagggatgta tgtcttgttt caagcatcac aaagaattca
     4141 agcactctaa atgttaatgg aagtacaatt cttaatcctg caccgattgt tgagatgccg
     4201 gatttctcgg atatactgaa agaagaggca caaacaggta taacatacac cggaaacaaa
     4261 acatttaaca gtggaaattt ctcgtttaat cagcctatct atgtaacggg agacttaact
     4321 cttaacagtg ggaatttcta ttttgattgt cctatttatg tggaggggaa attaaccatt
     4381 aacagtacta attttatagg taaaggtatc atttatgcaa agaacgatat caaggtaaac
     4441 agtgccagtt ttgcgacaac agccggcaca gtgagtcttt attcagggaa gaatatagag
     4501 cttaatagta tgtgggcaga tattaacgga tttatgtacg ctccaaacgg gactattata
     4561 atgaacagca tgtcttctca ggttaatgga agtgttatcg gaaatgaaat aaaactcaat
     4621 agcggatatc ttaaagttaa ctatggaggc ggtaattatg acggtcttcc ggatggcggc
     4681 agtgtgaaac tgatagaata attaaaaatt tgacaagccc tctataaaat gctaatatta
     4741 agttgttgca gggcaaagca gcaagagaaa gcaagaaatt ttggagggtg aaatacgaaa
     4801 tgaaatcatt aatcagactt agaatgagta cacaggatgc tcattatggc ggcaatttag
     4861 ttgatggagc aaggatgctg ggattattcg gggatgtggc aacagagctt cttatcagaa
     4921 atgatggaga tgaaggtttg ttcgttgcat atgacaatgt ggaattcttg gcacccgtat
     4981 atgcagggga atttattgaa gcagagggtg aaatagtttc aaaaggtaat tcttcaagaa
     5041 aaatggtatt tgaagcaaga aaggtaatag taccacgtac agacataagt gattcggcat
     5101 gtgatgtttt agaagagcct gtagtggtgt gcagggcatc tggcacatgc gtagttccaa
     5161 aggggaaaca aagacaaaaa taagaacttg aatatatgaa aaatccgcag actgagcatt
     5221 gaattgaaac cggcaagagc ttaaacggag ttttggcacc tgtatatgat ttcatgtaat
     5281 taaaatgtaa ttaaaatgaa atagaaatat aattcataaa gaatagaaat gtaattgtaa
     5341 caatatatac taaattcaac ataaaggtaa acattatata ttaaatattg acaataaccg
     5401 cagattaaat aattaaattg acaaaaaatt tatattaaat tataatcaat caagctgcaa
     5461 tacactataa gtgtaatata gctgaataca aaaaacatga gggtaaacaa ttgcaaaaat
     5521 caaactggaa acttggagag ttattattat atgcaggtaa aatttcgaaa gagcaattaa
     5581 atgaggcatt ggaggaccaa aagataaata agaagaagtt aggggaaatc atcgttgaca
     5641 aggcgtggtt aaaatttgaa gaaattgtag aaacattaga gctgcagctt ggatttccca
     5701 aagttgattt aagtaaatac gaaataaact ccaatgtagc tacattgata cctgaaagta
     5761 ttgtaaaaaa atacaaatta atagcaatag atataaaaga cgatatgctg cttgttgcta
     5821 tgtcagaccc tttgaacttt tttgctatag acgatataaa attatatacg aagatgggtg
     5881 taataccttg tctttcatct gttccggacg tggaaagagc tattgaaaga tactacagca
     5941 gcatattgac acaaaaagca ttaaatgagt tttcagatgc cttcattccg gcagaagatg
     6001 atacggattc tgaagaagaa atgcttgaag ttgcaactgc acccatagta aaattaataa
     6061 actcaataat cgaacaagct atgaggggca atgcatcgga tatacatatt gagccaagtc
     6121 acaacgatat cagagtaaga tttcgtatag acggtgattt aaaagaaata atggtattgc
     6181 cgaaatcaaa tttgtcagcc ataaccacca gggtgaaaat cataggaaga atggacatag
     6241 cagaaaaaag aatcccccaa gacggcagag ttgagatgaa attcaacggc aatgaagttg
     6301 atttaagaat ttccacactg ccaacggtat acggagaaaa aatagttata agaatattgg
     6361 acaaaagcaa tttcaacttt accaaagagg ggttaggttt cagcaaagac aatttagata
     6421 aattaaacaa aatgatagca cagccttacg gtatgctgtt agtaacggga cctacgggaa
     6481 gcggtaaaac taccacactt tatacggtac taagagagct gaatgttcaa actaaaaatg
     6541 taatcacagt agaagacccc gttgagtata aattaaaagg tataaatcaa acacaagtaa
     6601 atgtaaaggc aggattgaca tttgccgcgg ggctccgctc aattctcagg caggacccgg
     6661 atactataat gataggggaa ataagagact ccgaaacggc agaaattgca gtcagggcag
     6721 ctattacagg tcacttggta ctgtccacgc tgcatacaaa cgacagccca tcaaccatag
     6781 caaggcttat agatatggga ttggagccat acttggtatc atctgcagta ataggaatta
     6841 tttcacagag attagtaaaa ctattatgtc ctaaatgcaa agaaaaatat aaagcaagtg
     6901 atttagaaag aaaaatattg ggtatagatg gggataaaga aacaacgctt ttcaaaccgg
     6961 ttggttgtaa ctcctgtaac aatggataca gaggcagagc agcggttcac gaggtaatgc
     7021 ttgtaaatga aaatataaga agacttataa acacaggtgc aaacacagac gatataagaa
     7081 gtcaggctct aaaagacggg atgagtacct tacttcaatc agctgctgat ttagccttaa
     7141 agggagatac ttcattcgaa gaaataatga gagcgggata tacattataa gaaggacaag
     7201 ggggacaata tgaacttaat agaattatta agtattggag tagaaaaaaa tgcttctgac
     7261 gttcatttaa ctgtaggaat accacctaca tatagaatag acggtgcact aatttcatta
     7321 gtggaaagaa aattaactgc cgaagacaca ttatatttag ttaaacaagc attaaatgaa
     7381 acaagaatga ataaattaga tgagacagga gaaattgact tttcatattc tataccaagc
     7441 ataggcagat tcagagtaaa tgcatttaag caaagaggaa gctatgcaat ggttctaaga
     7501 ataattccgt tagagattcc attgatggat gaattaggac ttcctacaat cttaaatgat
     7561 ttatcaaaat taccaagagg tcttattttg gtgacaggcc ctacaggaag cggaaaaacc
     7621 actacattgg catctattat taataagata aattccgaaa gaagatgcca cataataaca
     7681 cttgaagacc ccttagagta tttacataaa cacaaaaaat caatagtaaa ccaaagagaa
     7741 gtaggctcag atactctaaa ttttgcaaat ggattaagag gggcattgag agaggacccg
     7801 gacgttatat tggtaggaga aatgagagat ttagaaacta tttccacagc cataacagct
     7861 gcagaaaccg gtcacttagt attatccact cttcatacaa atggtgctgc taaaacaatg
     7921 gataggataa tagatgtatt tccgccattt caacagcaac aaataagagt acaattagca
     7981 tcagttattg aagctgttat atcttgtaga tgataaaatg gaattgacag aaaacgacaa
     8041 ataagatttg acagtttt
//
LOCUS       DAOSHP010000074         7947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7841_length_7947_cov_9.151673, whole genome shotgun sequence.
ACCESSION   DAOSHP010000074 DAOSHP010000000
VERSION     DAOSHP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7841_length_7947_cov_9.151673"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1172
                     /gene="hflK"
                     /locus_tag="PLB33_07365"
     CDS             99..1172
                     /gene="hflK"
                     /locus_tag="PLB33_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOG63379.1"
                     /translation="MNFRNLIGNLFRMIKKPNDFQDFQNYDFNNQDFQAKNKPPSKPG
                     KFKFLLILPVLIVAAAVILSCQYTVEETEQAVVTTLGKVTSVESAGLHFKLPYPLQSV
                     TKVAVNKTQKLQIGYSSDSEAASYFSTSIPDEAKMITGDFNIVNIDFFIEWKISDPVK
                     FLYNSDEPALILKMVSQSSARSIIGSKDVDGVLTTEKSIIQAEIKEKIVDKLESYDLG
                     IQLLDVKIQDSEPPTEEVIQAFKEVETAKQEKETRINEALAYRNKTLPAAESKADKLI
                     REAESYRESKINEAKGDVARFNAMYTEYAKNKDITRTRMYLETIEEILPGVKVYIDSS
                     EGGVQKLLPLESFNINSNNGGEN"
     gene            1172..2062
                     /locus_tag="PLB33_07370"
     CDS             1172..2062
                     /locus_tag="PLB33_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOG63380.1"
                     /translation="MQDTKKKSKLGFLVKLIIFIVLVISVTNTAYIVRENEYAYVTRF
                     SKFVKIQDTAGLHFKLPLLDKIEKIPQYRMMYDIPPSEVLTGDKKTLVVDNFTVWQID
                     DPYTFMRTVSRISEMENRIDAIVYNAVKNTLGTMDQTTIINSDNSSIDDVNSKITELV
                     NAQLKNYGVSTVAVEIKRFDLPKDNESAVYNRMISERTQMAESFRAEGNLEASKIVNE
                     TDKEVAILLSKASARAEELKGQGESEYMKIIAEAYSTEDRMNYYEFIRSLEALKVTMK
                     GEKTLILPADSFIVRVLNGY"
     gene            complement(2148..2369)
                     /locus_tag="PLB33_07375"
     CDS             complement(2148..2369)
                     /locus_tag="PLB33_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HOG63381.1"
                     /translation="MAEIKYEITEELAVLSESSRGWTKELNMVSWNDREPKFDIREWA
                     PNHERMGKGVTLNREEMKKIKDILNEIDL"
     assembly_gap    2459..2558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2647..3573
                     /locus_tag="PLB33_07380"
     CDS             2647..3573
                     /locus_tag="PLB33_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOG63382.1"
                     /translation="MKKKDILLSVLIVFLWGVNFTVIKLGLSGVPSMLLVSLRYLLST
                     FPAIFFIEKPKTEMKYIVLYGLTVGVGQFSCLFYAMQIGMPAGIASVVLQTQAFISPV
                     FAMIFLNEKLKSRQIIGFIVSAIGLIFIAMASIGDGMNNVPVLAFILMLAAPTFWAVS
                     NIIARIASDKAAAKGEKLNMISMVVWSGLIPPIPMFGLALMFDTPETLLGILTNLSGI
                     SIFAALYLAYGATLIGYGIWSILIASYPLNKVAPITLLVPIVALLSARIILTEQLSPL
                     QWIGFIVILTGLAISNANININKFKKDLHFGR"
     gene            complement(3642..4808)
                     /locus_tag="PLB33_07385"
     CDS             complement(3642..4808)
                     /locus_tag="PLB33_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOG63383.1"
                     /translation="MLNLSFFGKSKVEYNGKEIGDRLGNKAIALICLLVLNERRYLSR
                     EKIIGYLWPDSNIEAAKYNLRYNLWLIKKNIAEDKNHNLFLKVDNDCCSINNNYEFNC
                     DIIDIMKFKPSREDSVESLLKLKKLFRGDLLEGCYFNKCDEFNDLIIYERINFEQRKV
                     RILQRLVEVYENDKRYDDCIEILYEIMEIEPYDEKIALKLMDIYQKSGKRAVAINYFN
                     KFSYSLSCDLGINPSNELKNKYNEIKMAVSGDEFNDETNYNVINKDTNLKIVSYCIKN
                     VEYFWMADVIDKIIDSGVEDCIQQLSQKQLLDLSSIQSSISKFCNDNIDIINYRREIM
                     DVCIINSFIKLVEAVCRKRNVTIVILNYCDIDEISANVVEYLRKIKIKGLDIIE"
     gene            complement(4826..6361)
                     /locus_tag="PLB33_07390"
     CDS             complement(4826..6361)
                     /locus_tag="PLB33_07390"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOG63384.1"
                     /translation="MRADILIKNGRCMTMKDREIMDWIAIKKSKIIAVGHGLEYESFV
                     GNETVVFDVNGSTVLPGFIDSHFHMVQTALNSVSLDLSGVKTHEEVGEKIKEASNSFM
                     GDYIRGIRISEGQFQEKVYPGRIFIDKYCNDVPVVLNSMEYQVSILNTCALLYFKIPF
                     TAEGIEMDRKKMPTGIFRKHANAILRTNILKNISDSTRKDATSEIMSTLLMNGITTVN
                     AMEGGFMYCDKDAEFVFEHGNDFPIDMVLFYQTMDIDRIKAMGLNRIGGCLYIDGTFG
                     ARTAALSFEYADCPGTMGSLRMSQQELNDFVIQCYKNNLQLALYTIGDRAIESALIAH
                     ENALYQTGITGLRHRLEHAELVNDNQIERAKGLNIIFSMQPSYETYWGGRGKMYEQRL
                     GENYKRTNPFKKIIDKGVVICGGSDSDVTEPRPLLGIHSAVNHPVAEHRVDIYEALKM
                     FTCNGAYGIFEENNKGTLEVGKLADVVILDQDILSVPSNDIDKIKVNVTIKSGEILYN
                     SLG"
     gene            complement(6539..7741)
                     /locus_tag="PLB33_07395"
     CDS             complement(6539..7741)
                     /locus_tag="PLB33_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOG63385.1"
                     /translation="MYKAMYKVDKAQITKLLIDLVNIESPYFEEDKIIEFIYRWFKQN
                     NIDSFIHEYHEAKVTGFKGKNIVVNLEGNKCGPVICLNGHLDTVKLCNGWTYNPKGEL
                     IGDRLYGVGALDMKSGCAATMIALKGFVENHKEFNGKIIATFVSVEEGPYGMGTNALI
                     EEGYIKDVDFSIITEPSSGFTGNPYPDVSFGARGGYGLEVEFFGKSSHAATPELGINA
                     AEDASRVITELKNVEYIEDPHLGKGSACVVAVESDGGACSVPDYAKIRLFWHIVMGES
                     EETITNEIRKAIERAQIKSSYQINFREAPSEGSRGFLPYTITGEEPITKIFLDAVEEV
                     CETKPTISYFQSIGDFNYLGSRLDAPAVIFGAAGGNFHGSDEYVELESVVKTAEVIYA
                     FLEKVLCP"
BASE COUNT         2585 a         1479 c         1210 g         2573 t
ORIGIN      
        1 atgtattcat tgtatcggtg tgaagcaagc cgattttgcg gcttgcactg atgataaaaa
       61 taagatagat aaatcaattg aatagtaaag gagtaagtat gaattttaga aatctaattg
      121 gtaatttgtt tagaatgatt aagaaaccta atgactttca agattttcaa aattatgatt
      181 tcaataatca agactttcag gctaagaata aacctccgtc aaagcccggt aagtttaagt
      241 ttttgttgat tttacctgta ttgatagtgg cagctgctgt aatactttct tgtcagtaca
      301 cggtagagga aacagaacaa gcagtagtaa caacccttgg caaggtaaca agcgtggagt
      361 cggcaggatt gcactttaag ctgccctacc ccttacagtc agtaacaaag gtagcggtaa
      421 ataaaacaca aaaattacag ataggctact caagtgatag tgaagctgcc agctattttt
      481 ctacatccat acccgatgaa gcgaaaatga taacaggaga ctttaacatt gtaaacatag
      541 acttttttat tgaatggaaa atttcagatc ctgtaaaatt tttatataat tcagatgagc
      601 cggcactaat tttaaaaatg gtatcacaat catcagcaag aagcataatc ggctcaaaag
      661 atgtagatgg tgttttgaca actgagaaat caataatcca ggcagagatt aaagaaaaaa
      721 tagttgataa acttgaaagc tatgatttgg gaattcagct cttagatgtt aaaattcaag
      781 acagtgagcc tcccacagaa gaggtaatcc aagcttttaa agaagtagaa acagcaaaac
      841 aggagaagga aacaaggata aacgaagcat tggcttacag gaataaaact ctgcctgcgg
      901 ctgaaagcaa agcagataaa ttaataagag aagctgaatc ttacagggaa tcaaaaatta
      961 atgaagcaaa aggtgatgtg gcacgattca atgcaatgta tacagaatat gccaaaaaca
     1021 aggatataac aaggacccga atgtatttgg aaactataga agaaattctt cccggcgtaa
     1081 aagtatatat tgattcatca gaaggcggag tacaaaaatt actgcctttg gaaagcttta
     1141 atataaattc aaacaatggg ggtgaaaatt aatgcaggat actaaaaaga aaagcaaatt
     1201 aggatttctt gtaaagttaa taatatttat tgtattggta atatcagtta ccaacacagc
     1261 ctatatcgtc agagaaaacg aatatgctta tgtaaccaga ttttcaaaat ttgtaaaaat
     1321 tcaagatact gcaggattgc atttcaagct tcccctcttg gataaaatag agaaaatacc
     1381 tcagtataga atgatgtatg acatacctcc ttcagaagta ctgaccggtg acaaaaaaac
     1441 attggtggta gataatttta cggtatggca gatagatgac ccctacacat ttatgagaac
     1501 cgtatcaagg atatctgaaa tggaaaatcg tattgatgca atagtataca atgcagttaa
     1561 aaacacattg ggaacaatgg accaaactac aattataaat tcagacaaca gctcaattga
     1621 tgacgttaat agtaaaataa cggaattagt taatgctcag ctgaaaaact acggtgtatc
     1681 tacggtagcc gttgaaatta aaagatttga cctgccgaaa gacaatgaat ccgcagtata
     1741 taacagaatg atttccgaaa gaacacaaat ggcggaaagc ttcagagcag aaggtaattt
     1801 ggaagccagc aagatagtca atgaaacaga caaggaagtg gcaatactgc tgtctaaagc
     1861 ttctgcaaga gcagaggagc ttaaaggcca aggcgaaagc gaatatatga aaattatagc
     1921 cgaagcttac tcaacggaag acagaatgaa ttattacgaa ttcatcagaa gcctggaagc
     1981 tcttaaggta acaatgaagg gcgaaaaaac tttaatattg cctgcagaca gctttattgt
     2041 aagagtactg aacggatatt aaagcgctct aaagcataca attacaaagc tcattctgtt
     2101 atagaatgag cttttccttt aaacaagaaa agccggatat attttgacta aaggtcaatt
     2161 tcatttagta tatccttgat ttttttcatt tcttctctgt ttagtgtgac tccttttccc
     2221 atcctttcat gattcggtgc ccattctctt atatcaaact taggttccct atcgttccag
     2281 ctgaccatgt ttaattcctt tgtccaacct ctcgatgatt cagaaagcac agctaattct
     2341 tcggttattt catacttaat ttcagccatt ttaccctcca tctttattct aaaacgtata
     2401 ataatatcaa taatatattc atgatattat accgcaaatc agtaataatt actacattnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aactttccaa ctaattttaa
     2581 tttaattata tatcaaaata ttttaaacta tgttataatc gaaatatcaa cattaggagt
     2641 aactaaatga agaaaaagga tatattgctg tcagtattaa tagtattttt atggggtgtg
     2701 aattttaccg ttattaaatt aggcttaagc ggagttccgt caatgctttt agtttcattg
     2761 aggtatctgc tttcaacatt tccggcaata ttttttatag aaaagccaaa aacagaaatg
     2821 aaatatattg tgttatacgg gctaacggta ggagtagggc aattttcatg cttgttttat
     2881 gctatgcaaa tcggaatgcc cgcaggcata gcttccgttg tacttcaaac tcaagctttt
     2941 atatcgcctg tttttgccat gatattttta aacgaaaagt taaaatcaag gcaaattatt
     3001 ggatttatag tatcagccat aggtttgata tttatagcaa tggcttctat cggagacggt
     3061 atgaataacg tacctgtctt agcttttatt ctgatgttag cggcacctac attttgggct
     3121 gtttcaaata taattgcaag aattgcttct gacaaagcag ctgcaaaggg tgaaaaactg
     3181 aatatgatta gtatggttgt atggtcagga ctgattccgc caattccgat gtttggtttg
     3241 gcactcatgt ttgacacacc tgaaaccctt cttggtatat tgacaaactt aagcggcatt
     3301 tcaatatttg cagcactata tttagcttat ggtgcaacat taatcggata tggtatttgg
     3361 agcatattga tagcatctta ccccctgaac aaggtcgccc cgattaccct attggttccc
     3421 atagttgcat tattgtcggc aagaattatt cttactgagc agctgtcacc attgcagtgg
     3481 ataggattca tagttatttt aacaggccta gcaatttcta atgcaaatat taatataaat
     3541 aagtttaaaa aagatttgca tttcggaagg taaactcgtt caacaaaaaa cgtatataat
     3601 aaaattatag ctgcattcaa ctgaactgca gcttataata attattcaat tatatctaat
     3661 cccttaattt taatttttct aagatattca acaacatttg ccgaaatttc atctatgtca
     3721 caatagttta aaatcacgat agtcacattt cttttacgac agacggcttc caccagcttt
     3781 ataaaagaat ttataataca cacatccatt atttctcttc tataattgat tatatcaata
     3841 ttgtcattgc aaaattttga tatactgctt tgtatagagc ttaaatctaa caattgtttt
     3901 tgactcaatt gttgaataca atcttctaca cctgaatcaa taattttatc aattacatca
     3961 gccatccaaa aatattctac attcttgata caataggaaa caatttttag attggtatct
     4021 ttattaatca cattataatt agtttcatca ttaaattcgt cacctgatac agccattttg
     4081 atttcattat atttattttt aagttcatta gagggattta taccaaggtc acaggataga
     4141 ctatagctga atttattgaa ataatttatt gcaactgcac gtttcccgct tttctggtaa
     4201 atatccataa gttttaatgc tattttttca tcataaggct caatttccat tatctcatat
     4261 aatatttcaa tacagtcatc atatctttta tcattttcat aaacttcaac taaacgctga
     4321 agtatcctta ccttgcgctg ctcaaaattt attctttcgt atataatgag gtcattaaat
     4381 tcatcacact tgttaaaata gcaaccttcc aataagtctc cgcgaaaaag tttcttaagc
     4441 tttagtaagc tttctacaga atcctctctg cttggtttaa atttcataat atcaataata
     4501 tcacaattaa attcataatt attgtttata ctgcaacagt cattgtccac cttcaaaaat
     4561 aggttgtgat ttttatcctc agctatattt ttctttataa gccataaatt ataacgcaag
     4621 ttatactttg ctgcctcgat gttgctatcc ggccacaagt atccgataat tttctccctg
     4681 cttaaatatc ttctctcatt taacactaaa agacaaatca aagctatggc cttatttcct
     4741 aaacggtctc ctatttcttt accattgtat tcaactttag attttccaaa aaaacttaga
     4801 ttcaacatat cattttcctt tttatttacc caaggctgtt atacaatatt tcaccagatt
     4861 taatagttac gtttaccttt atcttatcta tatcatttga aggtactgaa aggatgtctt
     4921 gatcaagaat tacaacatct gccaattttc ctacttctaa agtaccttta ttgttttctt
     4981 caaaaatacc atatgcgcca ttgcaagtaa acatttttaa tgcttcatat atatcaactc
     5041 tgtgttctgc taccgggtga tttacagcag aatgaatccc caaaagaggt cttggctctg
     5101 taacatcact atctgagcca ccgcatataa caacaccttt atcgattatc tttttgaacg
     5161 gattagttcg tttgtaattt tctcctagcc tttgctcata catttttcct cgtccgcccc
     5221 aatatgtttc atatgatggt tgcatagaaa aaataatatt taaacctttc gccctttcaa
     5281 tttgattatc attaactaat tcagcatgtt ccaatctgtg acgaagacct gttataccgg
     5341 tttgatacag tgcattctca tgtgcaataa gtgctgattc tattgctctg tcaccgattg
     5401 tatacaatgc taactgcaga ttgtttttat agcattgtat tacaaaatca tttagctcct
     5461 gctgtgacat acggagactg cccatggttc ccggacaatc tgcatattca aaggaaagag
     5521 ctgctgttct tgcgccaaag gttccatcga tatagagaca tcctccaatc ctgttaagcc
     5581 ccattgcttt aattctatca atatccattg tctgataaaa aagcaccata tctatcggga
     5641 aatcatttcc atgctcaaaa acaaactctg catccttgtc acagtacata aatccgcctt
     5701 ccatggcatt tacggtagta ataccattca ttaaaagagt agacataatt tcggaagttg
     5761 catctttcct tgtgctatcc gatatgtttt ttaatatatt tgttctaaga atagcattag
     5821 catgttttct gaagattcct gtaggcatct tttttctgtc catttcaatt ccttcagctg
     5881 taaaaggtat tttaaaatat aacaaggcac aagtattaag aatgcttacc tgatactcca
     5941 tggaattaag aacgaccggt acatcattac agtacttatc aataaatatt cttcccggat
     6001 atactttttc ttggaattgt ccttctgaaa ttcgaattcc gcgaatataa tctcccataa
     6061 agctattgga ggcttcttta attttttcgc caacttcttc atgagttttt actccactca
     6121 aatctaaact tacagaatta agagctgtct gaaccatatg aaaatgacta tcaataaatc
     6181 ccggaagtac cgtactaccg ttcacatcaa aaacaactgt ttcatttcca acgaaagatt
     6241 catattctaa gccatgtcct accgcaatta ttttactttt tttaatggca atccagtcca
     6301 taatttctct atctttcatt gtcatgcatc ttccattttt aatcaaaata tccgcacgca
     6361 tcttaattcc tccaaataat gtcgtttcat ttttatgcag ttgaataatt aaatataaca
     6421 aatattatac cagtataaaa ccgatttttc aatagaataa aaaagccggt aaagctttta
     6481 tttaattaat gataaaagtc taagaaaagc ttgatggctt ttcttagacc ttaaaaaatt
     6541 atgggcacag taccttctca agaaaagcat aaataacctc tgcagttttt acgactgatt
     6601 ccaattccac atattcatca cttccatgga agttaccgcc tgcggctcca aaaattacag
     6661 caggtgcatc caatctggag cctaaataat taaaatctcc aatactttga aaataagata
     6721 tagtaggttt tgtttcgcac acttcttcaa cagcgtccaa aaagattttt gttataggtt
     6781 cttctcccgt aatagtataa ggcaaaaaac ccctggaacc ttcagaaggt gcttctctga
     6841 agttaatttg ataactgctt ttaatctgtg ctcgctcaat tgcttttctg atttcatttg
     6901 taatggtttc ttcactttct cccattacaa tatgccaaaa caatcgtatt tttgcataat
     6961 caggaacact gcaggcacca ccgtcgcttt caacagccac cacacaggca gaacccttcc
     7021 cgagatgggg gtcttcaata tattctacat tttttaattc ggttattact cttgaagcat
     7081 cttctgctgc attaattccc aattcgggag tggctgcatg ggatgatttg ccaaaaaatt
     7141 caacctcaag accatatcca ccccgagctc caaagctgac atccggatat ggatttccgg
     7201 taaagcctga agatggctca gtgataattg aaaaatctac gtcctttata tagccttctt
     7261 caataagggc attagttccc atgccatacg gaccctcttc aacagaaaca aatgttgcaa
     7321 ttattttccc gttaaactct ttatggtttt cgacaaatcc cttcaatgcg atcatagttg
     7381 ctgcacagcc tgacttcata tccaatgctc caactccata tagtctatcg cctattagtt
     7441 ctcccttcgg attataagtc caaccgttac aaagcttaac agtgtctaag tgcccgttaa
     7501 gacatattac aggaccacac ttattgcctt ctaaatttac aacaatattt ttacctttaa
     7561 aaccggtaac ttttgcttca tgatactcat gaatgaaaga atctatatta ttttgcttaa
     7621 accagcggta gataaactct ataattttgt cttcctcgaa ataaggactt tcaatattga
     7681 ctaagtctat cagcaatttt gttatttgag ccttatcgac tttatacata gctttataca
     7741 tacgctcacc tactgtcctt taacattttt aattatatca ttttgcacta tcagatttct
     7801 cccctaatca atatctgctg ctttttctga aacttcttca taccaaacaa acttcccggt
     7861 atatgcataa ataattgcaa taacaggagt tatataagcc aggaacgcat atgggaaata
     7921 ctctaaggta tttactccaa gtactcc
//
LOCUS       DAOSHP010000075         7920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7882_length_7920_cov_8.312397, whole genome shotgun sequence.
ACCESSION   DAOSHP010000075 DAOSHP010000000
VERSION     DAOSHP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7882_length_7920_cov_8.312397"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..723)
                     /locus_tag="PLB33_07400"
     CDS             complement(265..723)
                     /locus_tag="PLB33_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOG63386.1"
                     /translation="MNELKQKAYIFATIFTLSNRLQTLGDEFDENFTTKQWLFILAVS
                     RFKEPPTITEVANFIGYSRQNAKRIAADLQKAGHIILQKDENDARALRIIINPGCIEH
                     FKKRDKQEIEFLEQIFTGFDAELTNSVYKGFVKLDKNIKEMAQKKNLLYE"
     gene            complement(963..1385)
                     /locus_tag="PLB33_07405"
     CDS             complement(963..1385)
                     /locus_tag="PLB33_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOG63387.1"
                     /translation="MEYKRFDNTILARIDKGEEILEKLMEICEKEHVKLANVNALGAI
                     GEFTIGLFDTSEKKYYSANYTGDYEIVSFTGSISTMDGKLYNHIHLSAGARDNKVYGG
                     HLNKAVVSATCEMFIHIVNGTVNRKFDEEVGLNLLDFN"
     gene            complement(1402..2430)
                     /locus_tag="PLB33_07410"
     CDS             complement(1402..2430)
                     /locus_tag="PLB33_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63388.1"
                     /translation="MLLFRIGPRHLFLRTENIKEVSNFLVTKLKGEVVEFWEGFVKAS
                     EDSTLCFITGIDHEKTYVEDAKEIVLVNEASSPILCSIISNPVSDLLQKVDMGPASIV
                     MRIPPGNADKIIEKTKEIFDGQEVDWIEGIGIGEKDDTIISFTRQELSRAVTDFLDTK
                     LLISKPVNEVKDRLRLEGLRLITHSLDDSQWYELRINIYDSYGNYQQHYERLMFILSK
                     LEIGMVLGESWTKDFAVILYSVLTYQVRLFTFHTPIEIKKILMALEYTADGKRLVDFD
                     LYYKNKKVYWYEVNKNRGKGIDKSDEAKNQRQNLYKKLKPEDIKKLEKMEKDNLDALK
                     KVLSGSKN"
     gene            complement(2441..3064)
                     /locus_tag="PLB33_07415"
     CDS             complement(2441..3064)
                     /locus_tag="PLB33_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOG63389.1"
                     /translation="MKNIKDIYKGLLSLINYPHISIDFLKDLKGPLLLHISDTPSEIY
                     NNIFKIIDIIKPQYIVHTGDLADNIKLKIQKDRINSYCKGVKELVEGLEKNDSKVYYL
                     MGNHDDYEVVSQFTKKGEILEEGLLPIDNLTITVGHYYKEHSFKSDFNLYGHSFEPGH
                     YKKDGTIGLNGVLNINIIDLSNKEVFHINYPNGTNHLRCMKTKKIGL"
     gene            complement(3270..3914)
                     /locus_tag="PLB33_07420"
     CDS             complement(3270..3914)
                     /locus_tag="PLB33_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOG63390.1"
                     /translation="MKENLTELVFILDRSGSMSGLETDTIGGFNSMLEKQKKESGEAF
                     VTTVLFDDKYELLHDRYDIKGVNLLTEKEYYVRGSTALLDAIGITINNIGKALSDTNE
                     EDRPGKVLFVIITDGMENSSREFSYEKVKKMVEHQKSKYSWDFIFLGANIDAIKTANS
                     FGISADMATNFVSDSVGTQLNYKIVSEVVSSFRKGKNVDKSWKDEIEKDYKKRG"
     gene            complement(3989..>4387)
                     /locus_tag="PLB33_07425"
     CDS             complement(3989..>4387)
                     /locus_tag="PLB33_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase III inhibitor"
                     /protein_id="HOG63391.1"
                     /translation="PTLIKTTKDKKSLEDIVSQLDETFSEMLLRLIDEKEMTDVETYK
                     KANIDRKLFSKIRTDKFYRPRKTTALALCIALELNLDQTKDLLARAGYALSPSSKFDL
                     IVEYFIEQGIYNIFEINEALFAFEENLLGV"
     assembly_gap    4388..4487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4488..6175)
                     /locus_tag="PLB33_07430"
     CDS             complement(<4488..6175)
                     /locus_tag="PLB33_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOG63392.1"
                     /translation="MKTCTLTINNKKITVEAGTTLLEAAKSAGYFIPTLCNHPDQEIK
                     ANCRICVVEDKSAGKFVASCSTKVYDGAEIHTNTKEVRELQRGILELILANHPNDCLN
                     CIRNESCELRKLCERFHVRNYNLNHETDIYPIDNTSGVLVRDRNKCIKCMRCVEACQE
                     TQMVGILSPSYRSVNTNISAGFGENYDETLCTFCGQCSAVCPVGAIYEYDETEKVWEA
                     LHDPKKHVIVQTAPAVRVGLGEEFGMPRGSKVTGKMVSALKMLGFDKVFDTDFAADLT
                     IMEEGYELLSRIKNGGTLPMITSCSPGWINYIEGFYPELLDHLSTCKSPQQMFGAVAK
                     TYYAKKAGIDPADIFTVSVMPCTAKKFECKRPEMNSSGYRDVDVALTTRELARLIKNA
                     GIDFTNLEDEDFDSIMGSSTGAAVIFGATGGVMEAALRTVYEVVTGKTLEKIEFDEVR
                     GLEGIKEATIDLDGTPVKVAVAHGLANANKVMEMIKNGEADYTFIEIMSCTGGCAGGG
                     GQPVGANKSIKARRIDTIYNIDKEMPIRKSHDNPEVQQLYEEFLGEPNGHLSHKL"
     gene            complement(6184..7416)
                     /locus_tag="PLB33_07435"
     CDS             complement(6184..7416)
                     /locus_tag="PLB33_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOG63393.1"
                     /translation="MGKSLSILTKNFKNINVDTIDDYLSAGGYDGLKKVLSLTPAEVI
                     EVVKASGLRGRGGAAFPTGKKWEQAARVKEDSYILCNADEGEPGTFKDRYFLEYDPYR
                     IIEGMTIAAYAVGGREGYIYIREEYGRQKEIFLKAIEAAKAAGFLGENILGTDLSFDI
                     KVVSGAGAFICGETTALIQSIEGGSGRPRIKFQRTAVSGLFNRPTLANNVESYCVVTT
                     LFNNDAQEYLSHGTEKSKGTKVISLCGNVENPGVYEVPFGITLREIIYDIGGGIKDGR
                     DFKFAQIGGASGPLIPKSMLDIPYSYEDFGKEGYSLGSGAVLVADDTNSVADFMVTVQ
                     EFFVHESCGKCTPCREGNRQLLKLAHKIADKKVTVEDFLTVKRIANVMDKCSFCGLGT
                     AAPTAMVTAIQHFYDMYN"
     gene            complement(7401..7904)
                     /locus_tag="PLB33_07440"
     CDS             complement(7401..7904)
                     /locus_tag="PLB33_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOG63394.1"
                     /translation="MSKNLLDANQYEEIDKILEKHDMSKHNLVGILSDVQAMSPEHYI
                     TKEQAEYISDKLGIFLSNVYDTITFYAALSDKPKGKYVIQICNSTCCKVMKYEKIKDA
                     IEKELKVGIGEITPDGVFSFEYSSCFGACDISPAIRIGEKVYGNLDETKVIDIIKNYR
                     GGTWENL"
BASE COUNT         2212 a         1683 c         1113 g         2812 t
ORIGIN      
        1 ctattacata taattacatc atgttcataa atatacgcaa catgttactt atataatacg
       61 taacatgttt acatttgtca agagcttttt aaaaattttt ttaaattatt ttcagtaata
      121 agtggcatat tttttatttc aaaaacttaa atattttata tatactcata ccgataggca
      181 ttcttatata aaatagggca atccaactgc aataaattct taaattatgc gtagaatgcc
      241 ctattctcta ttccttaaat tgtcttattc ataaagtaaa ttcttttttt gggccatctc
      301 cttgatattt ttatccaact taacgaaacc tttgtaaaca ctatttgtta attctgcatc
      361 aaacccggta aaaatttgtt ccaaaaactc tatttcttgt ttatcccttt ttttaaagtg
      421 ttctatacat cccggattaa ttataattcg taaggccctt gcatcatttt catctttctg
      481 caatataata tgtcctgctt tctgtaagtc tgcagcaatt cgttttgcat tttgacgtga
      541 atatcctata aaattcgcca cttcggttat agtgggcggc tctttaaatc tcgatactgc
      601 taatatgaaa agccactgct tagtagtaaa gttttcgtca aattcatcgc ctaatgtctg
      661 cagcctgtta gataaggtaa aaattgttgc aaaaatatat gctttctgtt taagttcatt
      721 catcaatttc cctccagtat atgcaacatg ttacctatat tgtatatggc tttctgctct
      781 ttgtcaagaa aaaaagacaa agtataaaac tttgtccgag ttgttagggt atcccattat
      841 gtcatcctga gcttaagcga aggatctcag gttttataag atgagattct tcgagctaaa
      901 gctctcagaa tgacacgtag cttattcata gcaatgacaa tcattatata ctttgtctaa
      961 ctctagttaa agtcaagcag gttaagtccg acttcttcgt caaattttct gttaaccgta
     1021 ccgtttacta tatgtataaa catttcacat gtggcgctta caaccgcctt attcaaatga
     1081 cctccataaa ctttattgtc cctggcaccg gcacttaaat ggatgtggtt atatagttta
     1141 ccatccattg tagaaatact ccccgtaaat gatactattt cataatctcc tgtataattt
     1201 gcagaatagt attttttttc acttgtatca aataatccaa tcgtaaattc tccaatcgct
     1261 cccaaagcat ttacatttgc cagtttaaca tgttcctttt cgcaaatttc cattaacttc
     1321 tcaagtattt cttcaccttt atcaattctt gccaatattg tattatcaaa tcttttatat
     1381 tccatttttc actctcttcc tttaattttt agagccggat agaacttttt ttaaggcatc
     1441 caagttatcc ttctccattt tttcgagttt tttaatatct tccggcttaa gctttttata
     1501 caagttttgt ctttgatttt tagcttcatc agacttatct atacctttac ctctattctt
     1561 gttaacctcg taccagtaaa cttttttatt cttatagtac aaatcaaaat cgactaacct
     1621 cttgccatca gctgtatatt ccaatgccat taaaattttc ttaatttcta ttggagtatg
     1681 aaaagtaaac aatctcacct ggtaagtcaa tactgaataa agtatcacgg caaagtcctt
     1741 agtccagctt tctcccaata ccatgccgat ttccagctta gatagtataa acataagtct
     1801 ttcatagtgt tgctgataat tcccgtaaga atcataaata tttattctaa gctcatacca
     1861 ctggctgtca tccaagctat gagttattaa cctcagtccc tctaatcgca atctatcctt
     1921 aacctcatta acaggctttg aaatcaataa ttttgtatct aaaaaatctg taaccgctct
     1981 gctgagttcc tgtcttgtaa aagaaatgat agtatcatcc ttttctccaa ttccaatccc
     2041 ttctatccaa tcaacttctt gaccgtcaaa aatctccttg gttttttcaa ttattttgtc
     2101 tgcattaccg ggtggtatgc gcataactat tgaagcaggt cccatgtcaa ccttttgcag
     2161 caagtctgaa acaggattgc tgattattga gcagagtatt ggcgaagatg cttcgtttac
     2221 aagtacaatt tcctttgcat cctcaacata ggttttttca tggtcaatac cggtaataaa
     2281 acataacgta ctgtcctccg atgcttttac aaatccctcc caaaactcta caacttcccc
     2341 cttaagtttt gtaactaaaa aatttgaaac ttctttgata ttttcagttc ttagaaacaa
     2401 gtgccttggt ccgattctaa acagcagcat aacatacccc ctataagccg atttttttag
     2461 ttttcatgca tctcaaatga ttagtaccat taggataatt tatatgaaat acctctttat
     2521 ttgataaatc tattatattt atatttaata ccccgtttaa gccgattgta ccatcttttt
     2581 tgtaatgtcc cggctcaaaa ctatgaccgt aaaggttaaa atcagactta aatgaatgct
     2641 ccttgtagta atgccccact gtaattgtta aattatcaat aggtaaaagt ccttcctcta
     2701 aaatttcacc cttcttggta aattggctga caacttcata atcatcatgg ttgcccatca
     2761 aataataaac ctttgagtca ttcttctcca atccctcgac gagttcttta actcccttac
     2821 aatagctgtt gattctgtct ttttgaattt ttagcttaat attatcggcc aagtcaccag
     2881 tatgcacaat atattgcggt tttataatat cgattatttt aaaaatatta ttataaattt
     2941 ctgaaggggt atcgcttata tgaagcaata aaggtccttt taaatccttt aaaaaatcta
     3001 ttgaaatatg aggataatta attaaggata aaagtccctt ataaatatcc tttatgttct
     3061 tcattgcctg caattctcct ttttaatcaa attaagcagt cccccaatat gcaaattgaa
     3121 gttctgtcat cttgtcatta atctttattc agattttaat cattatatat aatttttatc
     3181 actaaagcaa tattaattta tggtgccaga atttatggtg ccaggcacca catcggtgcc
     3241 tggcaccatt cacagacacc attcacaatt tatccccttt ttttatagtc tttttctatt
     3301 tcatctttcc agctcttatc aacatttttc ccttttctga agctgcttac tacttcactg
     3361 acaattttgt aattcaattg tgttcctacg ctgtcgctta caaaattagt tgccatgtct
     3421 gcactgatgc caaaagagtt tgcagtctta atagcgtcaa tattggcacc tagaaaaata
     3481 aagtcccaag aatatttgga tttttgatgc tcaaccatct ttttaacttt ttcataagaa
     3541 aattctctgc ttgaattttc catgccgtcg gttataataa caaacaatac cttaccgggt
     3601 ctatcttctt catttgtatc ggacaaagct ttgccgatat tattgattgt aattcctatt
     3661 gcatcgagca gcgcggtaga acctcgcaca taatattcct tttccgtaag caagttaacg
     3721 ccttttatat catatctgtc atgaagtaat tcgtatttgt catcaaacaa taccgtggtg
     3781 acaaatgctt ctccgctttc ctttttctgc ttttcgagca tagaattaaa tccgcctatt
     3841 gtatccgttt caagaccgga cattgagccg cttctgtcca atatgaatac taattccgtt
     3901 aaattttctt tcatttcgat acctccaatc atttattgat tgaaggatat cacttttatg
     3961 aatataagtg gtcgctttaa atgcgacatt atactcctaa caaattttct tcaaatgcaa
     4021 ataaagcttc atttatttca aaaatattat aaataccctg ctcaataaaa tattctacta
     4081 ttaagtcaaa tttgctgctg ggagaaagag cataaccggc tcttgcaagc aagtccttag
     4141 tttggtctaa atttaattcc aaagcaatgc aaagagcaag agctgttgtc tttctcggcc
     4201 tgtaaaattt atctgtcctt attttagaaa aaagcttcct gtcaatatta gcttttttat
     4261 aggtttcaac atcagtcatt tccttttcat ctattaatct caaaagcatt tcagaaaagg
     4321 tttcatctaa ctgagataca atatcttcta aacttttttt atcctttgta gtctttatca
     4381 atgtcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagt ttatgagaca
     4501 aatgaccgtt tggctctcct aaaaactctt catacagttg ttgtacttca ggattatcat
     4561 gagattttct tatgggcatt tctttgtcaa tattataaat agtatcaatt cttcttgcct
     4621 taattgattt atttgctccc accggttgac cgccgccgcc tgcgcatcct cccgtacagc
     4681 tcattatttc gataaatgta taatccgctt caccattttt aatcatttcc ataactttat
     4741 tggcatttgc caatccatgg gcaacagcaa ctttaacagg tgtgccatcc aaatctatgg
     4801 tagcctcctt tattccttca aggcctctta cttcgtcaaa ctcgattttt tccaatgttt
     4861 ttccggtaac aacctcatac accgttctta aagcagcttc cattacacca cccgttgcac
     4921 cgaatataac ggctgccccg gttgatgaac ccattattga atcaaaatct tcatcctcca
     4981 ggttagtaaa atctattcct gcgtttttaa tcaacctggc aagttctctg gttgtaaggg
     5041 caacatcaac atccctgtat ccggaagaat tcatttcagg tctcttgcac tcaaattttt
     5101 tggcagtgca cggcataacc gatacggtaa atatatccgc gggatctatt cctgcctttt
     5161 tggcataata tgtttttgcc acggctccaa acatttgctg cggagacttg caggttgaaa
     5221 gatggtctaa cagttcagga taaaaacctt ctatataatt tatccacccc ggactgcagg
     5281 atgtaatcat tggtagggta ccgccgtttt taatcctcga caaaagctca tatccttctt
     5341 ccattattgt taagtctgca gcaaaatcag tatcaaaaac cttgtcaaat cccaacattt
     5401 taagtgccga taccatttta ccagtaactt ttgagcctct cggcattcca aattcttcac
     5461 ctaagccgac tcttaccgct ggagcagttt gaacgataac atgttttttc ggatcatgca
     5521 gggcttccca aaccttttct gtttcatcat actcataaat agcaccgacg gggcatactg
     5581 ccgagcattg tccgcagaat gtacaaagtg tttcatcata attttcacca aaacctgcac
     5641 tgatatttgt attaacagac ctgtaggaag ggcttaatat tccaaccatt tgtgtttctt
     5701 ggcatgcttc gacacatctc atgcatttta tgcatttatt tctatccctt accaatactc
     5761 ctgaagtatt atctatgggg tatatatcgg tttcgtgatt caaattgtag tttcttacat
     5821 ggaacctttc gcaaagcttt ctaagttcac aagattcatt gcgtatgcag tttaaacaat
     5881 cattgggatg attggctaat atcagctcca aaatacctct ttgcagctct cttacttctt
     5941 ttgtattcgt gtgaatttca gctccgtcat aaacctttgt agaacatgaa gcaacaaatt
     6001 taccggcact tttgtcctct accacacaaa ttctgcagtt tgcttttatt tcttggtcag
     6061 gatgattgca tagggtcggt ataaagtaac ctgctgattt tgctgcttct aataaggtag
     6121 ttcctgcctc tacagttatt ttcttattat ttatagtcaa tgtacaagtt ttcatgtctc
     6181 ctcctaatta tacatatcat aaaagtgttg tattgccgtt accattgctg taggtgcagc
     6241 agtacctaat ccgcagaatg agcatttatc cataacgtta gcaatgcgtt taacggttag
     6301 gaaatcttcc actgttactt ttttatccgc aattttatgt gccaatttca aaagctgcct
     6361 gtttccctca cggcaaggtg tgcatttgcc gcagctttca tgaacaaaga attcctgcac
     6421 ggtgaccata aaatctgcaa cgctgtttgt atcatcggcc accaatacag ctccggagcc
     6481 cagactgtaa ccttccttac caaaatcttc ataagagtat ggaatgtcta acatggattt
     6541 tggaataagg ggacctgaag ccccgcctat ttgagcaaat ttaaaatctc ttccgtcttt
     6601 tattccgccg cctatatcat atattatttc tcttaatgta ataccgaatg gaacctcata
     6661 aactcccggg ttttcaacat ttccgcacaa gcttataacc tttgttccct tgcttttttc
     6721 ggtaccgtga cttaagtatt cctgtgcatc attattaaac agggttgtaa caacgcaata
     6781 tgactcaaca ttattggcaa gcgtaggtct gttaaataaa cctgatacgg cagtcctttg
     6841 aaatttaatc cttggccttc cgctgccgcc ttcaatggat tgtataagag ctgttgtttc
     6901 gccgcaaatg aatgctcccg caccggaaac cacttttata tcaaaggata aatcagttcc
     6961 taaaatattt tctcccaaaa atcctgcagc cttagcagct tctattgcct ttaaaaatat
     7021 ttccttttgc ctgccgtatt cctctctgat gtatatataa ccttccctgc ctccgactgc
     7081 atatgctgca attgtcattc cttcaattat tctgtaagga tcatattcca gaaaatatct
     7141 gtctttaaat gttcccggtt ctccttcatc ggcattgcat aatatgtatg agtcttcttt
     7201 tactcttgct gcctgttccc atttcttgcc cgtaggaaaa gctgctcccc ctctgcctct
     7261 taatccggaa gccttgacaa cttctataac ctctgcagga gtcaatgaca acactttttt
     7321 taatccgtca taaccgcctg ccgacaagta atcatcaatt gtatcgacat taatattctt
     7381 aaaattctta gttaaaatgc ttaaagattt tcccatgttc cacctcgata gtttttaata
     7441 atatcaataa cctttgtctc gtcaaggttt ccgtaaacct tttctccaat cctgattgca
     7501 ggtgatatat cacaagcacc gaagcaagag ctgtattcaa acgaaaacac tccatcagga
     7561 gtaatttcgc ctattccaac ttttagttct ttttcaattg cgtctttaat tttttcatac
     7621 ttcattacct tacagcatgt agagttgcat atttgaatta catattttcc ttttggcttg
     7681 tccgatagag cagcataaaa tgtaatagta tcatatacat tgcttaaaaa tataccaagc
     7741 ttatcactga tatattctgc ttgctccttt gttatgtaat gctcgggaga catagcttgt
     7801 acatccgata atatccctac gagattatgc ttgctcatgt catgtttttc aagaatctta
     7861 tcaatttctt catactgatt tgcatctaat aagttttttg acatacactc ctccattttt
//
LOCUS       DAOSHP010000076         7898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_7909_length_7898_cov_8.111947, whole genome shotgun sequence.
ACCESSION   DAOSHP010000076 DAOSHP010000000
VERSION     DAOSHP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7909_length_7898_cov_8.111947"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLB33_07445"
     CDS             <1..405
                     /locus_tag="PLB33_07445"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG63395.1"
                     /translation="RNIVFPDMIRMYWKKFMLNVGFNQVTAVTRAPYGKVTNIETSLI
                     LFKEAMNEVLTIAKALNIDLREEDIEEFVALMDHFSPFGKTSMLQDVEAKRKTEVDYF
                     GGTVVELGKKLNIPTPVNHVLYCIIKSLEELY"
     gene            complement(497..1609)
                     /locus_tag="PLB33_07450"
     CDS             complement(497..1609)
                     /locus_tag="PLB33_07450"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOG63396.1"
                     /translation="MLYITKNHLKPGMVLAKDIYLYNRRNFNTFLLAKGQVLSSTYIN
                     RINFHNIDGVYIENDAFSDINVEPYIADDLKAESLQRISDTYYEFQVSSGKINDYMIN
                     RLSKTVKNIIIELLNKDDLSYNIMDFKNYDSYTFQHCLNVAMLSISTGISLKLSEQDL
                     LDLGLAGMLHDIGKMLIPIEILNKPSTLTKEEFEIIKTHPVNASKQLRNMVPHEVLRA
                     VEDHHERLDGTGYPHEKRDNNISLYARILSISDVYDSLISDRPYRKSIFPNEVVEYLM
                     GCADKQFDYNILKHFLKIIFAYPVGTFVKLSNEELAVVIKNHSENILRPIVRIIKNDG
                     TVGEDVDLLYDKDYMNITIIDMGYDLLSMYPLADSY"
     gene            complement(1644..2690)
                     /locus_tag="PLB33_07455"
     CDS             complement(1644..2690)
                     /locus_tag="PLB33_07455"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG63397.1"
                     /translation="MNENQNNIQYYDDEIDLRELIMALWKRKKMIIAFTLAVAILAAS
                     FSMFILSPVYKTSLDIIISMPEIYNTRYGEYKLPITTNEQYINLISSDDVLINTIKDM
                     NYDTEEISVENLNKRIAVIASNTKANTVQNSFTVTVSADNPEESHKLAKSLFENYIEF
                     LDVMVKERAINHYINLFSVELMALQNTLDVEKETLSKNEELLSQTPKVINTKSNVEIQ
                     SPLNDESNYIIPIETANPNYIKIENDIIQNKQSINSIESSINKTMEYIEELNVEKQAV
                     SKYYKNGRTEKLETSVIGIVENNVYLASQPAIPTQKSSPNNSLNTIIGAVLGGMVGVM
                     AALFMEYWFKKENN"
     gene            complement(2776..4056)
                     /locus_tag="PLB33_07460"
     CDS             complement(2776..4056)
                     /locus_tag="PLB33_07460"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG63398.1"
                     /translation="MESLKHVKLGILGMGTVASGLINIMEFNKDKVHGNINGELMVNK
                     VLVNNPNKIRNVNLPKEVYTTDAYEVINSDADIIVELIGGLSPAYEYIKAALNNKKHV
                     VTANKALIATYGEELHILAEKNNVKLMYEASVGGGIPILNTIRDNLCANEFEYIFGII
                     NGTTNYILTQMTENGLDYRQALKEAQALGFAEADPSSDVEGDDAAYKLSILSTLAFGQ
                     RIHLKDIPKEGITKISKEDIEYAKELGYNIKLLAAGIKKGNEIELNVQPAFIPAAHPL
                     STVKNEFNAVFLKGNAVGELMLYGKGAGSLPTGSAVLGDIIHIVKNAGSSYAKEGEKK
                     YVISNKGFKQYYINMKVIDKPGVLGSIASIFGKNKVSLASVVQRQKNGNDTVPLIFIT
                     HETERKNIDAALEETKKFDFVTELDSIIIVENFK"
     gene            4676..5038
                     /locus_tag="PLB33_07465"
     CDS             4676..5038
                     /locus_tag="PLB33_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008800843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOG63399.1"
                     /translation="MGKFVVKEVKTGFKFDLKATNGQVIASSEIYKTKASCLKGIDSV
                     INNAPIANLEDQTVENFKVEKNPKFEIFTDKAGEYRFRLKAKNGEIIATSEGYKAMDS
                     CKNGVESVRKNAANSTIE"
     gene            5179..5835
                     /locus_tag="PLB33_07470"
     CDS             5179..5835
                     /locus_tag="PLB33_07470"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HOG63400.1"
                     /translation="MKKSVKKIFLYVSGLLILAMGVNVSKAAQLGISPVSAIPYALEL
                     IWNIELGKAVFIFNIFLIALQIALLRKNFKPIQLLQIVCTFLFGLFVTYTGKEFLLFW
                     LPIPSLYIIKLLYLFVSIVIIGIGVSFYLIPDYIPLPAEGTMNSIVELSKGKLKFSNV
                     KVSVDSSMVAVSALLSIIFLGQLKSVREGTIIAALLIGKVVGFVFKRYKPQIVEWIEK
                     "
     gene            6003..7094
                     /locus_tag="PLB33_07475"
     CDS             6003..7094
                     /locus_tag="PLB33_07475"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOG63401.1"
                     /translation="MKYICSKCNTRMDVATKKPKCDCGGLWKLDYKPPKFDLSLVDKN
                     NWSIFRYRAFMPLLGNEWEKISLGEGLTPVIQFNEDVLLKMDYFMPTLSFKDRGAAVL
                     IAHCKAIGVDSVVQDSSGNAGNSVAAYCAKAGIDCEIFVPEGTSPKKIDMIKSHNAKV
                     NVVPGSRDNCANVCRAKSDKEGKYYANHVYNPFFYEGTKTYIYEVYEQLNRIPENIFI
                     PLGNGTLFIGIIKALEEFMESNIIDKMPNIIAVQSEYCDPFTKAFKIKASKPVKVISK
                     PTMAEGIAIGEPMRGEEILEYIYKYNIEVINSPETRILEARELLASKGIYVEHTTAAT
                     YAAYMKYCELKGRTPALIPMCGAGLKSDH"
     gene            7226..7813
                     /locus_tag="PLB33_07480"
     CDS             7226..7813
                     /locus_tag="PLB33_07480"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOG63402.1"
                     /translation="MAIKIFIDQGHNPPGFHNAGAEGNGLNEGEINFRAGIYLGNLLA
                     NDYRFEYMLSRPTPTTVLGTNTSTSLAERVRMANQWPANYFISLHCNANVNPSINGSE
                     MYIYQFNTQANWLAQHIMNGIVRNTRLRDNGIRENKSLYVLRNTNMPAVLVEMGYLTN
                     FNDAMILRDNLWNVAYGIYLGILNYFGFLPLNNIG"
BASE COUNT         2501 a         1338 c         1383 g         2676 t
ORIGIN      
        1 cgaaacattg tatttccgga tatgataagg atgtattgga aaaaattcat gctcaatgtg
       61 ggatttaacc aggttacggc agttacaagg gctccttacg gcaaggtgac aaacattgag
      121 acgagtttaa tattgtttaa ggaagcaatg aatgaagttc tgaccattgc gaaagcatta
      181 aacattgatt tgagggaaga ggatatagag gaatttgttg ccttaatgga ccacttttca
      241 ccctttggca aaacatccat gctgcaggat gttgaggcta aaagaaagac tgaagttgat
      301 tatttcggag ggacggttgt tgaattgggc aagaagctta atatacctac gcctgttaac
      361 catgtgcttt attgcataat taaatcatta gaagaattat attaagcatt aagaatataa
      421 ctaactttat gcggaactgc taaaacaaac atgggcgact aattgaatat cgcccttttt
      481 tactgttgga aacttattaa taagaatcag caaggggata catagaaagc aggtcatatc
      541 ccatatcaat tatcgtgata ttcatgtaat ccttatcata aagtaaatca acatcttcgc
      601 ctacagtgcc gtcatttttt ataattcgca ctattggtct taatatattt tcggaatgat
      661 ttttaattac taccgctaat tcttcgttgc ttagtttaac aaatgttcct accggatagg
      721 caaaaataat tttcaaaaag tgttttaaaa tattataatc aaattgtttg tcggcacatc
      781 ccatcaaata ctctacaact tcatttggaa agatagattt tctatatggc ctgtctgaaa
      841 ttaaagaatc gtacacgtcg cttattgaca atatccttgc gtatagactt atgttattgt
      901 ctcttttttc atgtggatat cctgtgccgt caagtctttc atgatggtct tcaactgctc
      961 ttaaaacttc atgtgggacc atatttctta attgctttga tgcattgaca ggatgtgtct
     1021 ttataatttc aaactcttct tttgtcagtg ttgacggctt attaagtatt tcaatgggta
     1081 ttaacatttt accgatatca tgcagcattc cggccaaacc taaatcaaga aggtcttgct
     1141 cgctgagttt caatgatatt cccgtagaaa tgcttagcat agccacattc aggcaatgct
     1201 gaaatgtgta gctgtcatag tttttaaaat ccataatgtt atatgataaa tcatctttat
     1261 tcaataactc tattataatg tttttaacgg tctttgacag ccggttaatc atataatcgt
     1321 ttattttccc cgaacttact tggaattcat aatatgtgtc actaatgcgt tgcagtgatt
     1381 ctgcctttaa gtcatcagca atatagggtt ccacattgat atcagaaaag gcatcgtttt
     1441 ctatataaac tccatcaata ttgtgaaaat tgattctgtt gatgtaagtg ctgctgagta
     1501 cttgtccctt agccaaaaga aaagtattaa aatttcttcg gttatataga taaatatctt
     1561 ttgccaatac catacccggc ttaaggtgat ttttagtgat atataacata gaattctcca
     1621 agcagtaata ttgtatagaa atattaatta ttttcttttt taaaccagta ttccataaat
     1681 aatgctgcca tgactccaac catacctcca aggacagcac cgattatagt attaagtgaa
     1741 ttgttgggac tggatttttg tgttggaata gcaggttgcg aagctaaata tacattgttt
     1801 tcaacaatac cgataacgct cgtttcaagc ttctcggtcc ttccgttttt atagtatttg
     1861 ctgaccgcct gtttttcaac attcaattcc tcaatgtact ccatggtttt gttaatcgag
     1921 ctttcaatag agttaatact ttgcttgttt tgaataatgt cattttcaat ttttatataa
     1981 ttaggattag ccgtttcaat aggaattata taattgcttt catcgtttag aggcgattga
     2041 atttcaacat ttgatttagt atttattact tttggagttt gtgaaagcaa ttcttcattt
     2101 tttgataagg tttccttttc tacatccaat gtattttgca aagccatcag ctcaacacta
     2161 aaaagattta tataatggtt tatggctctt tcttttacca taacatccaa aaattcaata
     2221 tagttttcaa ataaggattt tgcgagttta tgagattcct cagggttatc ggcagaaacc
     2281 gtaacagtaa agctgttttg tactgtgttt gccttagtat ttgatgctat tacagcaatt
     2341 cttttattca agttttcaac actgatttct tcagtatcat aattcatgtc ctttattgtg
     2401 ttaataagca catcatcgct gctgattaaa ttaatgtact gctcatttgt tgtaatagga
     2461 agtttgtact ccccgtatcg ggtgttgtaa atttcaggca tacttattat aatgtctaag
     2521 cttgttttat atacgggcga taatataaac atactaaatg atgctgcaag tattgcaaca
     2581 gccaaagtaa aggctattat catttttttt cttttccata aggccataat cagctctctt
     2641 aggtcaattt catcatcata gtattgtata ttattttgat tttcgttcat tattttactc
     2701 cttatttgtc ttattcatca aacaatatac attatatcag ttcagactgt tctgttcaat
     2761 aagatttacc gatttttact tgaaattttc aactattatt atgctgtcca attctgttac
     2821 aaaatcaaat ttcttagttt cttcaagggc agcatcaata ttttttcttt cggtttcatg
     2881 agtaataaat atcaagggaa cagtatcatt gccgtttttc tgtctttgca ctacagaagc
     2941 cagacttacc ttgttttttc cgaaaatact tgcaatactt ccaaggactc ccggcttatc
     3001 tataactttc atgttgatgt aatattgttt aaaacctttg ttggaaataa catacttttt
     3061 ctcgccctct tttgcatatg atgagcctgc atttttaaca atatgaatta tatctccaag
     3121 tactgcacta cccgtaggca gagagcctgc acctttgccg tacagcatta attcgccgac
     3181 tgcattacct ttcaaaaata cggcattaaa ttcattctta acggtcgata aagggtgagc
     3241 ggcaggaatg aatgcaggtt ggacattaag ctcaatttca tttccttttt tgattccggc
     3301 tgccaacagc ttgatattgt atcctaattc ctttgcatac tcaatatcct cttttgatat
     3361 tttcgtaata ccttcctttg gaatgtcttt aaggtggatt ctctgtccaa aagctaaggt
     3421 agacagtata gacagtttgt atgccgcatc atcaccttca acatcggaag aagggtcagc
     3481 ttctgcaaag ccgagagctt gtgcttcctt taaggcttgt ctataatcca gaccgttttc
     3541 agtcatttgc gtgagaatat aatttgttgt tccgttaata attccaaaaa tatattcaaa
     3601 ttcatttgca cagagattat ctcttattgt gtttagtatt ggaattcctc cacccacgct
     3661 tgcttcatac attaacttaa cattattttt ttctgccaat atatgcagtt cttctccgta
     3721 agttgcgata agagctttat ttgcggttac aacatgcttt ttgttattga gtgctgcctt
     3781 tatgtattcg taagctggac ttaatcctcc aattaattca actattatat ctgcatcgct
     3841 gtttattact tcatatgcat cggttgtgta aacttctttg ggcaagttaa catttcttat
     3901 tttattgggg ttgttaacaa gaaccttatt taccattaac tccccgttta tatttccatg
     3961 cactttatct ttattaaatt ccataatgtt aataagtcct gatgctactg tccccatgcc
     4021 taatattcct aattttacat gttttaatga ttccatttta atcctccctt gttcccggta
     4081 taaatacacg tgtactttta aaaaacacta tatcaatata gcacaatttt atattaagtt
     4141 caagaaaaat aatattccat tcatgaaagt cttcgggcaa gccataagaa tgatactcaa
     4201 ggggttaccc ccggcaacgc acataaatag cacccgaact tcttttcaat tcaaagccgg
     4261 gtgctattta tttgagtttt cttttccgta tcctgttgga atcacctcat ttgtcaatca
     4321 tctatattat caactttaca aatcttacct ctattttcat tcctcgatag acttttactg
     4381 cactttgaga tattcctata gaattctccc caaacaattt gattgcagcg accttataat
     4441 tactcacaga tttttcgtaa gactttgtat tgttgtataa ataatatacc caaaatttag
     4501 atgtttaaac agtaaacttt tataatacat attatgctat atataattat atgttactaa
     4561 ttgtgaaaaa gatgtagaat ttttaggaat catagttaga tataaaaaag ttcattgaca
     4621 cattttgaca aaattgatat actaaaaaaa gtctatcaat gaaaggagcg ggaacatggg
     4681 taaatttgtt gttaaagaag ttaaaacagg ttttaaattc gatttaaagg ctacaaatgg
     4741 acaagttatt gcaagctctg aaatttacaa gacaaaggca tcgtgtttga aaggcataga
     4801 cagcgttata aataatgcac ctattgctaa tttggaagat cagactgttg aaaattttaa
     4861 agtagaaaag aatcctaagt ttgaaatttt caccgacaag gcaggagagt acagattccg
     4921 cctaaaagcc aagaacggag aaattatagc tacatcggaa ggttataaag ccatggacag
     4981 ctgtaaaaac ggggtagaaa gcgtccgtaa aaacgcagca aattcaacta tagaatagta
     5041 atatgccata atgttacgaa gaacagcatc cgctgttctt tttttctaat ttcttaaaga
     5101 taaaaataat actatatatg acttattata tgttataata acattgaggt tcaaaaaaat
     5161 acatcggggg aagtttactt gaaaaagtca gtaaaaaaaa tatttttata tgtgagcggc
     5221 ctattaattc ttgcaatggg agttaatgtt tctaaggctg cacagcttgg tatatcaccg
     5281 gtcagcgcaa ttccttatgc attagaactt atatggaata tagagctcgg caaagctgtt
     5341 tttatattta acattttttt aattgcattg caaatagctt tattaaggaa aaatttcaag
     5401 cccatacaat tgctgcagat tgtatgcaca tttttattcg gtttgtttgt tacatatacc
     5461 ggcaaagaat tcttattatt ttggctgcca ataccatctt tgtatatcat aaaattatta
     5521 tatttgtttg tgagtatagt aattattggt ataggcgtat cattttatct tattccggac
     5581 tatataccgc ttcctgccga aggaactatg aattcaatag tcgaacttag taagggcaag
     5641 ttgaaatttt caaatgttaa ggtttcggtt gacagcagca tggtagctgt ttctgcttta
     5701 ctgtccataa tttttttagg acagctgaaa tcagtaagag aaggaactat catagcagcg
     5761 ctcttaatag ggaaggttgt aggatttgtc tttaagcgtt ataaaccaca aattgtcgaa
     5821 tggattgaaa aataatacaa aaaattttac aaatttttaa cgtgttaacc gcacgttgtc
     5881 atcctgagcg taagcgaagg atctcatgag attcttcgag ctaaagctct cagaatgaca
     5941 aaaatgttaa ccatagaaaa tataccaaat ggatataact atatgataag aaggtgaaac
     6001 aaatgaagta tatttgttct aaatgcaata caagaatgga tgttgcaaca aaaaagccaa
     6061 agtgcgactg cggaggactt tggaagctgg attataagcc ccctaagttt gacctttcct
     6121 tagtagacaa aaataattgg agtatttttc gttatcgtgc tttcatgcct cttttaggca
     6181 atgagtggga aaaaataagc ctgggagaag gtttgacacc tgttattcaa tttaatgaag
     6241 atgttttatt gaagatggat tacttcatgc ccaccttatc attcaaagac agaggggcag
     6301 cggttttgat agcacactgc aaagccatcg gagttgattc ggttgttcag gacagcagcg
     6361 gcaatgcagg aaacagtgtt gccgcatatt gtgcaaaagc cggcattgat tgtgaaattt
     6421 ttgtgcccga aggtacatcc ccaaagaaaa ttgatatgat taaatcccac aatgcaaagg
     6481 taaatgtagt tcccggatca agagacaact gtgcaaatgt ctgcagagca aagtcagaca
     6541 aagaagggaa gtattatgca aatcatgtgt ataatccttt cttttatgag ggtaccaaga
     6601 cctatattta tgaagtatat gaacagctta acagaattcc cgaaaatatt tttatacctt
     6661 tgggaaacgg caccttgttt atcggaataa taaaggcatt ggaggaattc atggaaagca
     6721 atatcataga taaaatgcct aatattattg cagtgcaaag cgaatactgc gaccccttta
     6781 caaaggcatt caaaataaaa gcttcaaaac ccgtaaaggt aatatccaag cctactatgg
     6841 cagagggaat tgcaataggt gagcccatga ggggagaaga aatattggaa tatatctata
     6901 agtataacat cgaggttatt aattctcctg aaacaagaat tttggaagca agagaactgc
     6961 ttgcttcaaa gggtatttat gttgagcata caacagcagc tacatatgca gcatacatga
     7021 aatattgtga attaaaaggg agaactccag ctttgattcc tatgtgcgga gcaggactca
     7081 agtcagacca ttagcgcgtt gggacacacc tctactaata aggacattct ttgatggcag
     7141 aggaatgtcc tcaaattaaa aaaaaatcac actttattat cgtacgaata aaatagttat
     7201 agcatataat aaaggaagtg attttttggc aatcaaaata tttatagatc aaggacacaa
     7261 tcctccggga tttcataatg caggggccga aggcaacggc ttaaacgaag gtgaaataaa
     7321 tttccgtgca ggaatttatc ttggaaattt attggctaat gattacagat tcgaatacat
     7381 gttatcaagg cctacaccaa ccacggtatt gggaacgaac acatcaacca gtctggcgga
     7441 aagagttcgc atggcaaatc aatggccggc aaattatttt atcagtcttc attgcaatgc
     7501 caatgttaat ccaagtatta acggctctga aatgtatata tatcaattta atacccaggc
     7561 taattggctg gcacagcata ttatgaacgg catagtaaga aacacacgct taagggataa
     7621 cgggataagg gaaaacaaat ctctttatgt attaagaaat acaaatatgc ctgctgtatt
     7681 ggttgaaatg ggttacttga ctaattttaa tgatgcaatg attttaaggg ataatttgtg
     7741 gaatgttgca tacgggattt atctaggcat tttgaattat tttggatttt tgccgcttaa
     7801 taatataggt tagaaaagga gagtatatta aaccaccggc tcaccggtgg ttttgacatt
     7861 ttagcttcct tgaactctgc ttgtatcgcc gaatctta
//
LOCUS       DAOSHP010000077         7827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8035_length_7827_cov_9.708827, whole genome shotgun sequence.
ACCESSION   DAOSHP010000077 DAOSHP010000000
VERSION     DAOSHP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7827
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8035_length_7827_cov_9.708827"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLB33_07485"
     CDS             complement(<1..410)
                     /locus_tag="PLB33_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63403.1"
                     /translation="MSKKFLTLISVVLIFTMIVTACSSGTGTPKEETIKIGWIGSLTG
                     DQAVWGTCESNTVKMLIEEVNASGGILGKQVEGIYYDTKGDAAEAVNATKRLASQDKV
                     VAIIGPNASGQAIAISSVLEEYKVPDISTVGTNP"
     gene            699..5015
                     /locus_tag="PLB33_07490"
     CDS             699..5015
                     /locus_tag="PLB33_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOG63404.1"
                     /translation="MEYFVGLDIGSTTVKIAVLDFFGKIVYKQYERHYSDITNTVTEI
                     INNSSEYLKDSFIRINVTGSGAIDVSKYLDIPFVQEVAASTRAIEKYIPSTDVVIELG
                     GEDAKITYLSGNLEQRMNGSCAGGTGAFIDQMAVLMKTDAAGLNELSRKHKTIYPIAS
                     RCGVFAKSDIQPLLNDGARQEDIAVSIFQAVVNQTISGLAQGRPIKGNVAFLGGPLYF
                     LSELRERFKETLNLTENNFVCPDDANYYVAIGAALMSIENKKISFNDFYDSIKTKIEN
                     SNFTYRKIDLNPLFKNKDEYNEFIERHNKHKAKRKNIKEYFGDAFLGIDAGSTTTKLV
                     LITKDGEILYSYYGSNEGNPLNSALDAVKGLYSQLHSGIKIAYTAVTGYGERLIKAAL
                     NVDIGEIETVAHYKAADFFLKGVDFIIDIGGQDMKSMVIKNGVIDSIMLNEACSSGCG
                     SFIETFAKSLDMTVEEFAQEATWSKNPVDLGTRCTVFMNSKVKQAQREGATISDISAG
                     ISFSVIKNALFKVIRLNNLDSLGEKIVVQGGTFYNDSVLRAFEQLTGREVVRPDIAGI
                     MGAFGAAVIAKNKYSEEKESIFVKAADLDNFSFNTSMERCGLCSNNCLLTVNKFSDGR
                     YYVSGNRCERGAEKYISITKKESLPNLYKYKTKRVFNYKPLPLSEARRGIIGIPRVLN
                     MYEDFPFWFTFFSELKYRVVLSGISSKKIYELGMGTIPSESVCYPGKIAHGHITDLIN
                     KKVKKIFYPCISYNIKEDESAGNHYNCPIVVSYPESIEANIDMKDVVFYKPFLNLNDK
                     ERLIEKLYRELGEAEGLTLFEIRSAAEKAYEELFSYKQDVRNEGERALTYISENNIKG
                     IVLAGRPYHIDPEINHGIPEMIESYGLAVLSEDSISDKGTVERPLRVIDQWSYHTRLY
                     KAATFVSNTDMLELIQLNSFGCGLDAVTTDQVREILEKNNKIYTVIKIDEITNLGAVR
                     IRLRSLLASMEEREKNGVVHKYSPSNFKRVIFTKEMKENHTILIPQMSPIHFQFISTA
                     AKMSGYRAVVLPAVDKKAVDAGLKVVNNDACYPSIIVIGQLINALASGKYDLNNTSLM
                     ISQTGGGCRATNYIGFIRKALKDSGYGDIPVISFNVVGLEKQPGFKLTAPFFNRLIMA
                     IVIGDTLMRTTYRTRPYEKIKGSINSLYEKWVKKAHMIIEKANIKEFKNLIQNIVTDF
                     DNVELDESIKKPKVGIVGEILVKFHPNANNDVFSLLEEEGAEVVVPDLLDFFSYTAYN
                     GVIKYDELLIGSKATKAINNIAVKIIEFYKRPSIDAFKNSKRFLTPVGIKELANKAKN
                     LLSLANQCGEGWFLTAEMIELLESGINNILCLQPFACLPNHITGKGMIKKLKEAYPWS
                     NIVPIDYDSGMSEVNQINRIKLMLTNAVRNMKQELDTALLNSIEENAPDSNLQNMKEI
                     EI"
     gene            complement(5041..6036)
                     /gene="argF"
                     /locus_tag="PLB33_07495"
     CDS             complement(5041..6036)
                     /gene="argF"
                     /locus_tag="PLB33_07495"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOG63405.1"
                     /translation="MAVNLKGRSFLTLMDFTPEEIRYLLDLSADLKRKKRSGSTDYLL
                     KGKNIVLLFEKTSTRTRCAFEVAALDEGAHVTFLDSGSSQMGKKESLEDTAKVLGRFF
                     DGIEYRGYEQKVVEDLAKFAGVPVWNGLTDVDHPTQILADMLTIEEHVAKPLNKVKVV
                     FAGDIRNNMSYAWMYGCAKMGMHFVAFGPKVLADQLDKEILKKVNEVAEYTGAKIEIS
                     DSIDSVKGADVIYTDVWASMGEEALIPDRVKLLTPYKVTMDLLKATENEDVIFMHCLP
                     AFHDFETKLAKEQKELGYDIREVTDEVFRSRHSVVFDEAENRLHTIKAVIVATIG"
     gene            complement(6119..7336)
                     /gene="arcA"
                     /locus_tag="PLB33_07500"
     CDS             complement(6119..7336)
                     /gene="arcA"
                     /locus_tag="PLB33_07500"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOG63406.1"
                     /translation="MNKSINVYSEIGKLKKVLLHRPGRELENLMPEYLDRLLFDDIPY
                     LKIAQQEHDAFANIFRENGAEVVYLEDLVVESLINDEVNSKFIDEYIEEAGIKEDRET
                     GILKEYFLSFSDKKDMVLKMMEGIRKSEIKDYRGPGLADFLSNQYPFIIDPMPNLYFT
                     RDPFAALGSGVSIHKMMTATRNRETLFGKYIFNYHPTYKDTKLFYDRTEDYSLEGGDI
                     LVLNKETIAVGISQRTNPNAIEKFANIILTEESSFKKVLAIDIPKTRAFMHLDTVFTM
                     VDYDKFTIHPNIKSDIVVYVLKKIDGKMDITEERATLEQVLEKELNIDKIKLIKCGGD
                     SVIDASREQWNDGSNTLCISPGEVIVYSRNYVTNKILEEEGIKIHIMPSSELSRGRGG
                     PRCMSMPLIRENV"
     gene            7562..>7827
                     /locus_tag="PLB33_07505"
     CDS             7562..>7827
                     /locus_tag="PLB33_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63407.1"
                     /translation="MFGSHVTRLGTNVIRNRTNIRGIRNGRIALYILSIDISATLAHT
                     NSDVPTGGVERPIIRLRINTIPRCTGSIPKLPISGIKNGIVIVMT"
BASE COUNT         2560 a         1425 c         1360 g         2482 t
ORIGIN      
        1 tttgggtttg ttccaaccgt agaaatgtca ggaaccttgt actcttcaag aacagaggaa
       61 atagctattg cctgaccact ggcattagga ccgataatcg ctacaacttt gtcctgtgat
      121 gcaagtctct ttgttgcatt tacagcctca gctgcatcgc ccttggtatc ataataaata
      181 ccttcaactt gtttacctag tataccgccg cttgcattaa cttcttcaat caacattttt
      241 accgtgttgc tttcacatgt tccccaaact gcttggtctc ctgttaaaga tccaatccag
      301 cctattttga ttgtttcttc ttttggtgta cctgtgccgc tactgcatgc agtaacaatc
      361 attgtgaaaa tcaaaaccac acttataagt gttaaaaact ttttactcat aaaaccctcc
      421 tgattcataa ttattagttt acttacaaaa ctgaggtcat gtaaacgtta ttattgtcca
      481 tataattata agcttttttt atgttaatta caagttacat tattgttata ttatcccaaa
      541 aatcatccaa atgttccaat aagccacaaa tttctttata catcggtacc ccacattggt
      601 gccaggcata aattaagttg attttgagca ttaaaatgat ataattattt atatttattg
      661 acagtacaat gcaaattgac ttaactccgg aggccattat ggaatatttc gtgggacttg
      721 atatcggctc aactacggta aaaatagctg tattagattt tttcggcaaa attgtatata
      781 aacaatatga aagacactac tcagacatta caaatacagt gacggaaata ataaacaatt
      841 catctgaata tcttaaggac agttttatca gaataaatgt tacaggttca ggtgctattg
      901 atgtgtcaaa atatttggac atccccttcg tacaagaagt tgctgcctct acaagagcta
      961 tagaaaagta tataccctcc acagatgtag taatagagtt aggcggagaa gatgcaaaaa
     1021 taacatatct ttcaggaaat ttagagcagc gcatgaatgg ctcctgcgca ggaggtacag
     1081 gtgccttcat tgaccaaatg gcagtattga tgaagaccga tgctgcaggt ttaaatgaat
     1141 tgtcgagaaa acataaaact atttatccta ttgcttcacg ctgcggtgta tttgccaaat
     1201 cagatatcca gcccttgtta aatgacggag caagacaaga agatattgcg gtatccattt
     1261 ttcaagcagt tgtaaatcaa accataagcg gacttgccca aggaagacct attaaaggaa
     1321 atgtagcgtt tttaggagga cctttgtatt tcttgtccga gcttagagaa agatttaaag
     1381 aaacactgaa tcttacggaa aataattttg tatgtcccga tgatgccaac tactatgttg
     1441 caattggcgc agcattaatg tccattgaaa ataaaaaaat atctttcaat gatttctatg
     1501 atagtattaa aacaaaaata gaaaattcaa attttacata tcgcaaaata gatttaaatc
     1561 ccttgttcaa aaataaagat gaatataatg aattcattga gcgtcacaat aaacataaag
     1621 ctaaaagaaa aaatataaaa gaatactttg gagatgcatt tttaggaatt gatgcaggtt
     1681 cgacaaccac taaattagta ctgattacaa aagacgggga aattctttat tcttattacg
     1741 gaagcaatga aggaaatcct ctgaattctg cccttgatgc agttaaagga ttatattctc
     1801 aattacattc aggcattaaa attgcatata cggcagtgac cggttatgga gaacgcttaa
     1861 taaaggctgc attaaatgtt gatataggag aaattgaaac cgttgctcac tataaagctg
     1921 cagatttctt tttaaaaggc gtggacttta ttattgacat aggcggccaa gatatgaaaa
     1981 gtatggttat taaaaatgga gttattgatt ccattatgct taatgaggca tgctcctcag
     2041 gctgcggctc ctttattgaa acatttgcga aatctctgga tatgactgtt gaagaatttg
     2101 cgcaagaagc aacatggtcc aaaaatcctg tagaccttgg cacaagatgc actgtattta
     2161 tgaattcaaa ggtaaagcaa gctcaaagag aaggagcaac cataagcgac atttctgccg
     2221 gaatttcatt ttcggtgatt aagaatgcct tatttaaagt aataagactt aataacctgg
     2281 attcattggg agaaaagatt gttgttcagg gaggaacatt ttacaacgat tctgttcttc
     2341 gcgcatttga gcaattaacg ggacgagaag ttgtaagacc tgacattgca ggtattatgg
     2401 gagctttcgg tgctgccgtg attgccaaaa ataaatatag cgaagaaaaa gaaagtattt
     2461 ttgttaaagc agccgacctt gataattttt ctttcaatac atccatggag cgctgcggtc
     2521 tttgctcaaa caattgctta ttaactgtca ataaattttc cgacggcaga tattatgtat
     2581 ccggcaatcg ctgtgaaaga ggtgctgaaa agtatatttc aattactaaa aaggaatctt
     2641 tgccaaacct gtacaaatat aaaaccaagc gtgtttttaa ttataaaccg ctgcccttga
     2701 gtgaagcaag aagaggaatc atcggcattc ctagggttct taatatgtat gaagattttc
     2761 ccttctggtt tacatttttc agcgaactta aatacagagt tgtgctttca ggaatatcaa
     2821 gcaagaaaat ttatgagctt ggaatgggaa caataccatc cgaatcagtt tgttatcccg
     2881 gtaaaattgc acatggacac ataaccgacc ttattaataa aaaggtaaaa aaaatattct
     2941 acccttgtat ttcttataat attaaggaag atgaaagtgc aggcaatcac tataactgtc
     3001 ctattgtagt atcttatccg gaatcaatcg aagctaacat tgatatgaag gatgtagtat
     3061 tttacaagcc attcttgaat ctgaacgata aggagagact cattgaaaaa ctttacagag
     3121 aattaggaga agctgaaggt ttaactctgt ttgaaatccg ttcagctgca gagaaagctt
     3181 atgaagaatt gttctcttac aagcaggatg taagaaatga gggggaaaga gcactaacat
     3241 atataagcga gaataatata aaaggtattg ttcttgcagg aagaccctac catatagatc
     3301 cggaaatcaa ccacggcata cctgaaatga tagaaagtta cggtttagct gtattgtcag
     3361 aggattccat cagtgataaa ggcactgttg aaagacccct tcgtgttatt gaccaatggt
     3421 cttatcatac aagactttac aaagcggcaa catttgtttc aaatactgat atgttggaat
     3481 taatccagct taattctttc ggatgcggct tggatgccgt aacaaccgac caggtaaggg
     3541 agatacttga aaaaaacaat aagatataca cggtaattaa aatagatgaa attactaacc
     3601 ttggggcagt cagaattcgt ttgcgctcat tgctggcttc tatggaggaa agagaaaaaa
     3661 acggagttgt tcataagtat tctccctcaa acttcaaaag ggttatcttt acaaaagaaa
     3721 tgaaggagaa tcatactata ctgatacctc aaatgtctcc cattcatttt caatttatta
     3781 gtactgctgc aaaaatgtcc ggttataggg ctgttgttct tcctgcagta gataaaaaag
     3841 ccgtggatgc aggattgaaa gtcgtaaaca atgatgcctg ctatccttca attatagtaa
     3901 taggacagct tattaatgcc ttggcatcgg gaaaatacga tttaaacaat acctcgctta
     3961 tgatttctca gacaggagga ggctgccgtg ctacaaatta tataggattt ataagaaaag
     4021 cactgaagga ctcaggttac ggagatattc cggtaatatc atttaatgtg gtaggtcttg
     4081 aaaagcaacc gggatttaag ctgactgctc ccttttttaa cagacttata atggctatag
     4141 taatagggga tactctgatg cgtaccactt atcgcacaag gccttatgaa aaaataaagg
     4201 gctccataaa cagcctgtat gaaaaatggg ttaaaaaagc tcacatgatt atagaaaaag
     4261 caaatattaa agaatttaaa aatctgatac aaaatatagt tacggatttt gacaatgtag
     4321 aattagatga aagcataaaa aagccaaaag tcggaattgt aggcgaaata ctcgtaaaat
     4381 ttcatccgaa tgcaaacaat gatgtgtttt cactattgga agaagaaggt gctgaagttg
     4441 tagtaccgga tcttctggat tttttttcct acaccgctta caacggagtt ataaaatatg
     4501 atgaactgct gattggcagc aaggcgacaa aggcaataaa caatattgca gtaaaaatta
     4561 tagagtttta taaaagaccg agtattgatg cattcaaaaa ttctaaaagg tttttaactc
     4621 ctgtcggtat taaggaattg gcaaataagg ctaaaaattt attgtcctta gccaatcaat
     4681 gcggagaagg gtggttttta acggcagaaa tgattgagtt attagagagc ggtattaaca
     4741 acatcttgtg tcttcagcca tttgcatgtc tgccgaatca cattacgggc aagggaatga
     4801 ttaaaaagct aaaggaagct tatccctggt ctaacatagt acccatagat tacgattcag
     4861 gaatgagcga agtaaatcaa ataaacagaa ttaaactgat gcttaccaat gctgtaagaa
     4921 atatgaaaca ggagctcgat acagctcttt taaactctat tgaagaaaat gcacctgatt
     4981 ctaatttaca aaatatgaaa gaaatcgaaa tataaagcac ctctaaaagg tgctttatta
     5041 ttatccgatt gttgctacaa taaccgcttt aattgtatgt aatctgtttt ctgcttcatc
     5101 gaaaacaaca gaatgtctgc tcctgaaaac ctcatccgta acttccctta tatcataacc
     5161 taattccttt tgctcttttg ccaatttagt ttcaaagtca tggaatgcag gaaggcaatg
     5221 catgaatatt acatcttcat tttctgttgc ttttaaaagg tccatggtta ctttgtatgg
     5281 tgtgagtaat tttactctat cgggaatcag agcctcttct cccatggagg cccaaacgtc
     5341 ggtatatatt acgtcggctc ctttaacact gtcgatactg tctgatatct caatttttgc
     5401 tccggtatat tcagcaactt cattaacctt ttttaatatt tctttatcta attggtcagc
     5461 aagtaccttc ggtccgaatg ctacaaaatg cattcccatc tttgcacagc cgtacatcca
     5521 tgcatatgac atattgtttc ttatatctcc tgcaaatact acttttacct tatttaaagg
     5581 ttttgccaca tgttcttcta ttgtaagcat gtctgccaat atctgtgtcg gatgatccac
     5641 gtcggttaag ccgttccaaa cgggaacgcc tgcaaattta gccaaatctt ctacaacctt
     5701 ttgctcgtat cctctgtatt caataccgtc gaagaatcta ccgagtacct ttgcagtatc
     5761 ttccaaagac tcttttttac ccatctgtga gcttcctgaa tccaggaaag ttacatgtgc
     5821 gccttcatct aatgcagcaa cttcaaaagc acatctggtt cttgtagatg ttttttcaaa
     5881 caataaaaca atgtttttac ctttcaataa ataatcggta gagcctgacc tcttttttcg
     5941 ttttaaatcc gctgataagt caagtaagta tctgatttct tccggtgtaa aatccattaa
     6001 tgtcaaaaaa cttcttccct ttaaatttac tgccatttac atcctccatt attaatttta
     6061 tatcctttgt atgcttaata tatcaaaaca ttttaaatat ttcaattctt ttacaacttt
     6121 atacgttttc cctgattaag ggcatgctca tacatcttgg tccgcccctg ccccttgata
     6181 actcagagct tggcattata tgaatcttta ttccttcttc ctcaagtatt ttattcgtaa
     6241 cgtaatttct tgaataaaca attacttcac ccggtgatat acataatgta ttggagccgt
     6301 cgttccattg ctccctggag gcatcaataa cactgtcgcc gccgcactta attaatttaa
     6361 tcttatcaat atttagttct ttttccaaca cctgttcaag ggtggctctt tcctctgtaa
     6421 tatccatttt tccatcaatt tttttaagaa cataaacaac tatatcagac ttaatatttg
     6481 gatgtattgt gaatttgtca taatcgacca tggtaaatac cgtgtccaaa tgcataaaag
     6541 ctctggtttt aggtatgtct attgccagta cctttttaaa ggaactctct tctgtcaata
     6601 ttatgtttgc aaacttttca attgcatttg gattagttct ttgtgaaata cccactgcta
     6661 tagtttcttt attcagcact aatatatctc cgccttcaag agaataatcc tcagttctat
     6721 cataaaataa ttttgtatcc ttatatgtag gatggtagtt aaatatatac ttcccgaata
     6781 aagtttccct gtttcttgtt gcagtcatca ttttgtgaat agatacaccg ctgcccagtg
     6841 cagcaaacgg atctcttgta aaatataaat ttggcatagg gtcaattata aatgggtatt
     6901 gattgcttaa aaagtcagct aaaccgggac ccctgtaatc ttttatttca gactttcgta
     6961 tcccttccat catttttagg accatatcct tcttatcgga aaaagacaga aaatattctt
     7021 tcaagatacc ggtttctcta tcttccttta tacctgcttc ctcaatatac tcatcaataa
     7081 actttgaatt aacttcatca ttaataagag actcaacgac caaatcttcc aaataaacaa
     7141 cttctgctcc attttctcta aaaatgtttg caaaagcatc atgttcctgc tgtgcaattt
     7201 ttaaatacgg aatatcgtca aaaagcaatc tgtcaagata ttccggcatt aaattttcta
     7261 attctcttcc aggtctgtgc aataaaactt tttttaattt cccgatttcg gaatatacat
     7321 ttatcgactt attcacatta tcaccaccta catatatact aataatcttt ccattgtttg
     7381 tcaagcttat ttaacatatt tataaagtag cattatattt atgcactttt aattaaggct
     7441 tgttaaacaa atcatcaggg gttgccgcct taatttatcg taaataaaaa acagttgaaa
     7501 tacaactgtt tcatataaat aaaatttaat ggccaggtaa gtattttgtt atttaagcat
     7561 catgtttggt agccatgtta caaggctcgg tacaaatgtt atcaggaaca gaacaaatat
     7621 aaggggaata aggaacggca gaattgcctt atacattctt tcaatagata tatctgcaac
     7681 cttggcacat acaaacagtg acgttccaac aggcggtgtg gaaagaccta tcataagatt
     7741 gagaatcaac actatcccaa ggtgtaccgg atcaattcca aaactcccca taagcggaat
     7801 caagaacggt atagttattg tcatgac
//
LOCUS       DAOSHP010000078         7750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8132_length_7750_cov_9.952307, whole genome shotgun sequence.
ACCESSION   DAOSHP010000078 DAOSHP010000000
VERSION     DAOSHP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8132_length_7750_cov_9.952307"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1383)
                     /gene="rseP"
                     /locus_tag="PLB33_07510"
     CDS             complement(142..1383)
                     /gene="rseP"
                     /locus_tag="PLB33_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOG63408.1"
                     /translation="MLTLIASILVFSAIVLVHEYGHYKAAKSVGIKIYEFAIGMGPTV
                     FKKEKKGIVYSIRAVPFGGFVQMEGEEEDSRTPTSFSTKTIAQRAKVIAAGPVMNFVL
                     ALVLFLIIAFSYGVYGNHIDYIDENSNEYKAGLRTGDKIISVNGERTYIWEDIFYELV
                     SPEESSYTLEYERNGQIDKVNIDKNYRKIIGVTSTIVNGKTTTTVTPSDMTSPAYKAG
                     IMDGDIVIKINDIPVNTWDELTTQVENSNKTVKVTVDRKGEIIDYNIDLVEQISVRFN
                     TELEKSFMTAISSSMYKTVYYIELMFKTIRLLVTGKLGSDALGGPVMVISMVGEAAQN
                     GILSLLSLAAFISINLGFMNLLPFPALDGSKLVFLAIEGIRGKALPVEKEGYIHFIGF
                     VFLIGFMLFITYKDLLRVFRI"
     gene            complement(1399..2166)
                     /locus_tag="PLB33_07515"
     CDS             complement(1399..2166)
                     /locus_tag="PLB33_07515"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOG63409.1"
                     /translation="MGRRVLTGFAGGAFIALITYLGGSLYDFVFLGITCIAIYELSKV
                     FFNDGFDYGAVMNYMFAIALFIEKITENRHFFDFFLFLYISIIFLIYIFNKKVTVKKV
                     SEIIFMGTYVVFFMYHMMLMNNSKFVWLVYIIAFGTDTFAYFTGKFFGKNKLYPEVSP
                     NKTVEGAIGGIIGSTILSLIYFNYLSINKYFYIIIFSVSASVFSMAGDLTASKIKREY
                     KIKDFGNLLPGHGGIMDRFDSVLFVAPTVYYFILHFI"
     gene            complement(2177..2899)
                     /locus_tag="PLB33_07520"
     CDS             complement(2177..2899)
                     /locus_tag="PLB33_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOG63410.1"
                     /translation="MNIEEKALSGKIPEHIAVILDGNRRWAKQKNLPVQAGHREGALR
                     VTDLVRNCGDLGVKYLSVYAFSTENWRREKKEIDYLMNLLVEFVFKELENLHKNDVKI
                     TMMGNMEDLPSKTRKEVERAIELTKNNKTLHLNIALSYGSRNEIVNAVKNIIKDYENN
                     KIDIEEINEESFKKYLFTNDMPDPDLLIRTSGELRLSNFLLYQLAYTEFYFTEVLWPD
                     FNKNELIKAIENYQQRKRRYGN"
     gene            complement(2952..3134)
                     /locus_tag="PLB33_07525"
     CDS             complement(2952..3134)
                     /locus_tag="PLB33_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63411.1"
                     /translation="MDSLIGYPLKEALQKLESLNVIINIVKVKGSNRRFNNLEKPYVI
                     KENYSDDTVTLYVSYF"
     gene            complement(3136..3693)
                     /gene="frr"
                     /locus_tag="PLB33_07530"
     CDS             complement(3136..3693)
                     /gene="frr"
                     /locus_tag="PLB33_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOG63412.1"
                     /translation="MYKDLIIKSEESMKKSINFLKEELATIRAGKANPKLIDKIQVSY
                     YGTMTPLNQIANISVPEPRMLLVQPWDANSLKDIERALLTSDLGINPTNDGKVIRLII
                     PVLTEERRKELLKLVKKEIENSKVAVRNIRRDTNEEIKRMEKQSEISKDDQKRAEDEM
                     QKLTDKYINLIDEIYKDKEKEVLEV"
     gene            complement(3695..4405)
                     /gene="pyrH"
                     /locus_tag="PLB33_07535"
     CDS             complement(3695..4405)
                     /gene="pyrH"
                     /locus_tag="PLB33_07535"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOG63413.1"
                     /translation="MCKYKRIVLKISGEALSGSAGHGINSEIVNKISNVIKRINEMGV
                     EVAIVVGGGNFWRGACAKDIDRTTSDYMGMLGTIINGLALQDALEKIDVETRLQTAIE
                     MRQIAEPYIRRRAVRHLEKGRVVIFSGGSGNPYFSTDTTAALRAAEINAEIILLAKNG
                     VDGVYSDDPNKNPDSVRYEELNYIDVLNKGLKIMDSTATSLCMDNKIPILVFGIETPE
                     NIEKIILGENIGTIIKEV"
     gene            complement(4469..5383)
                     /gene="tsf"
                     /locus_tag="PLB33_07540"
     CDS             complement(4469..5383)
                     /gene="tsf"
                     /locus_tag="PLB33_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOG63414.1"
                     /translation="MEITASMVKELREKTNAGMMDCKKALKETNGDIEKAIVYLREKG
                     LLQAAKKSSRIASEGLTTALISADEKKGVVLEVNSETDFVAKNEEFKSFVEETAKAVL
                     DNEPKDLEALKAVVLNDGKTVQEALTDKIAKIGENMSLRRFGEVKVDKGVVVDYIHGG
                     GKISVVVAMESEGDKEALKVLGKDIAMQVAAMSPKYISMEDVDAEFIAQEKEILTAQT
                     LNEGKPQNIVEKIVEGRLNKELKEICLLEQAFVKDADLTVKKVISDTAKKLGSDIKIT
                     GVLRYEVGEGIEKKSENFADEVAKQMGL"
     gene            complement(5451..6233)
                     /gene="rpsB"
                     /locus_tag="PLB33_07545"
     CDS             complement(5451..6233)
                     /gene="rpsB"
                     /locus_tag="PLB33_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOG63415.1"
                     /translation="MAIISMKKLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTSDKVDEAYNFIKEVVSNGDEILFVGTKKQAQESTKSEAIRCGMHYVSQRWLGGMLT
                     NYSTIRKRIDRLNELKTMEEDGTFDLLTKKEVFKLRNEAERLEKFLGGIKNMDKLPGA
                     LFVVDPRKERIAVQEAAILGIPVVAIVDTNCDPDEVDYVIPGNDDAIRAVKLLTATIA
                     DAVIEGKQGVQMTHEDTEDEVTEELEDLEELEELEEVEEELE"
     gene            complement(6414..>7750)
                     /locus_tag="PLB33_07550"
     CDS             complement(6414..>7750)
                     /locus_tag="PLB33_07550"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOG63416.1"
                     /translation="GVKVKGEGNVGLVTYIRTDSFRLSDEAVENAKQFILSNYGEKYH
                     KFRVYKKAKKNESKVQDAHEAIRPTSLLRTPEKIKDSLTTDQYKLYSLIWKRALACQM
                     ADAEYDVTKVLFNNNDNIFESNGKVLVFDGFRAVYGYADEKDEKMFPEVKINEVLDIS
                     KVIPEQHFTQPPARYTEASLIKDLEEKGIGRPSTYAPTITTITSRDYVAKEKGYLFPT
                     DMGFLVTDMMEKYFPKIVNEKFTADLENHLDDVASGNINWRNVISNFYESFKKELDVA
                     ENEMKEIEIKDEVSDVKCDNCGAFMVIKSGRFGKFLACPNYPECKNTKPLNGKEPPEE
                     VDETCEKCGSKMLKRKGKFGEFLACSNYPKCKNTKPIIKTIDAPCPRCGKQIAIKFSK
                     AGRRFYGCTGYPDCKYISWYEPTDKKCPECGEMMVLKNNKQTCINKKCKKSE"
BASE COUNT         2153 a         1579 c         1051 g         2967 t
ORIGIN      
        1 ctgattattt tatccatact tttctatcct ctccttataa aagatgatat tcttcactgc
       61 tttcagaata acaaaatgtt tatgaataca aaacaggtgt gtcaaatctc aataccagat
      121 gacgacaccc ccgtcccctt gtcatattct aaatactctc aataaatcct tataagttat
      181 aaacaacata aaacctatta aaaaaacaaa acctataaag tgtatataac cttctttttc
      241 aacaggcaat gctttacctc ttattccttc tattgccaga aataccagct tgcttccgtc
      301 taatgccggg aaaggaagta aattcataaa gcccaaatta atgcttataa aggctgcaag
      361 actgagaagt gataaaattc cgttttgggc tgcttctccg accatagata ttaccattac
      421 aggtccgcct aaggcatcac tgcccaattt gcccgttaca agcagtctta tggttttaaa
      481 cataagttca atataatata cagtcttata cattgaagaa gatatagccg tcataaatga
      541 tttttcaagc tcagtattaa atcttacaga tatttgctcc accaaatcta tattgtagtc
      601 tataatttct ccttttctgt ctacggtaac ctttactgtt ttatttgaat tttcaacctg
      661 ggttgtcagc tcatcccaag tgtttacagg tatgtcatta atttttatga caatatctcc
      721 gtccataatt cctgccttgt atgcaggaga agtcatatct gaaggagtta cggttgtagt
      781 ggtttttccg tttacgatag ttgaggtaac tcctattatt ttccgatagt ttttgtcgat
      841 attaacttta tcgatttgac catttctttc atattccaat gtgtaagaac tttcctcagg
      901 agatactaat tcataaaata tatcttccca tatatatgtt ctttcaccgt taacggaaat
      961 aattttatct ccggtcctta atcctgcttt gtattcattg gagttttcat ctatgtaatc
     1021 tatatgatta ccataaactc catatgaaaa agctattatt aaaaacaata caagagccaa
     1081 cacaaagttc atcacgggac ctgctgcaat tactttggct ctttgagcta tggtttttgt
     1141 actaaagctt gtaggtgttc ttgaatcttc ttcttcacct tccatttgaa caaacccgcc
     1201 gaacggaaca gctctaattg aatatactat tccttttttt tctttcttaa atacggttgg
     1261 acccattcct atggcaaatt catatatctt tataccaacg ctttttgcag ctttataatg
     1321 accatattca tggacaagaa caatagcaga aaaaacaaga atagacgcta ttaatgttaa
     1381 caatatagta acctccgttt atataaaatg cagaataaaa tagtacactg taggtgcaac
     1441 aaataaaaca ctgtcaaatc tgtccattat gcctccgtga ccgggcagaa ggttcccaaa
     1501 atcctttatt ttgtattctc ttttaatttt agatgctgtc aaatcgccgg ccattgaaaa
     1561 tacagatgca cttacactaa atattataat gtaaaaatat ttatttatgc ttaaataatt
     1621 aaaatatatc aatgataaaa tcgtgcttcc gataataccg ccgattgcac cctcgacagt
     1681 tttattcggg cttacctcag gatataattt atttttgccg aaaaatttac cggtaaaata
     1741 tgcaaatgtg tcagtgccga aagctataat atataccaac catacaaatt ttgaattatt
     1801 cataagcatc atatgataca tgaagaatac cacatatgta cccatgaaaa ttatttctga
     1861 aacttttttt acagttactt ttttattgaa tatatatatt aaaaatatta tagatatgta
     1921 taaaaacaag aaaaaatcaa aaaaatgtct gttttcagta attttctcaa taaataaagc
     1981 aattgcaaac atgtaattca taacggctcc ataatcaaag ccgtcattaa aaaaaacttt
     2041 tgataattca taaatagcta tgcatgttat gcccaaaaaa acaaagtcat ataaggagcc
     2101 gcctaaatat gttataagtg ctataaatgc tccgcctgca aaaccggtta acactcttct
     2161 acccatagta caccgcctaa tttccgtatc ttctttttct ttgctgatag ttttcgattg
     2221 ctttaattaa ttcattttta ttaaaatcag gccataaaac ttccgtgaaa taaaattccg
     2281 tatatgcaag ctgatacaag agaaagttgc ttaaccttaa ttctccgctg gtcctgatta
     2341 ataaatcagg gtctggcata tcattagtaa ataagtattt tttaaagctt tcctcattaa
     2401 tttcttctat gtcaatttta ttgttttcgt aatctttaat tatatttttt actgcattca
     2461 ctatctcatt tcttgagccg tagctcaatg caatattcag atgaagtgtt ttgttgtttt
     2521 tagtaagctc aattgcgcgc tctacctctt ttctcgtctt gcttggcaaa tcctccatat
     2581 tccccatcat tgttattttt acatcgtttt tatgaaggtt ttcaagctcc ttaaatacga
     2641 attcaaccaa gagattcatt aaataatcta tttctttttt ttctcttctc caattttcag
     2701 ttgaaaaagc ataaacagat agatatttta ctccaaggtc accgcaattt cttactaaat
     2761 cagtaactct taaagctcct tctctatgtc ctgcctgtac gggcaggttt ttttgttttg
     2821 cccatcttct gtttccatct aatataactg caatatgttc aggtattttc cctgacaacg
     2881 ctttttcttc aatattcata ataaacctca caattccaaa agtacggggt acatagaggt
     2941 gtccccgtac cttaaaagta agatacgtac aatgttacgg tatcatctga atagttttct
     3001 ttaattacat atggtttttc taagttattg aatcttctat tactgccttt tactttcact
     3061 atattaataa taacatttaa actttctaat ttttgaagtg cttcttttaa tggataaccg
     3121 attaaactat ccaacttata cctctaatac ttctttttct ttatctttat atatttcgtc
     3181 tataaggttt atgtatttgt cagtaagctt ctgcatttca tcttctgccc ttttttggtc
     3241 atctttggat atttcgcttt gtttttccat ccttttaatt tcttcgttag tatctcttct
     3301 gatatttcgt actgcaactt ttgaattctc aatttctttt ttaaccagtt ttaaaagttc
     3361 ttttcttctt tcttccgtaa gcaccggaat tatcagcctt ataacctttc cgtcatttgt
     3421 cgggttaatt cccaagtcgg aagttaacaa agctctttca atatctttta atgaatttgc
     3481 atcccaaggc tgaacaagaa gcattctcgg ttccggtacg gaaatattag ctatttgatt
     3541 taatggtgtc attgtaccgt aataacttac ttgaatttta tcaattaatt tcggattagc
     3601 tttgcctgct cttatggttg caagctcttc cttaagaaaa tttattgatt ttttcattga
     3661 ttcttctgat ttaattatta aatctttata catactatac ctccttaatt attgttccta
     3721 tgttttctcc caaaattatc ttttcaatat tttcaggagt ttctattcca aaaaccaaga
     3781 taggaatttt attatccata cataatgaag ttgccgttga gtccattatt ttcagacctt
     3841 tattcaagac atctatataa ttaagttctt cgtatcttac agaatcagga tttttattcg
     3901 ggtcgtcgct gtaaacacca tcaacaccgt ttttagctaa aaggataatt tctgcattta
     3961 tttcagcagc tcttaatgca gctgtggtat cagtggaaaa gtatgggttc ccggaacctc
     4021 ccgaaaatat tacaactctt cccttttcca aatgtcttac tgctcttctt cgtatgtaag
     4081 gttctgcaat ttgtctcatt tcaattgctg tctgtagtct tgtttccaca tcaatttttt
     4141 ctagggcatc ctgaagagca agtccgttta ttattgttcc aagcattccc atataatcag
     4201 atgttgttct gtctatgtct ttggcacagg cacctctcca aaagttcccg cctcccacaa
     4261 caattgcaac ttctactccc atttcattta tacgcttaat aacattggaa attttgttaa
     4321 ctatttctga atttatgccg tgacctgcgc tgccggacaa ggcttctccg ctgattttta
     4381 atactattcg tttatatttg cacattttat cctcctgata atttttattt taatggagaa
     4441 ccattggttc tccattgtga ttacaggatt ataaacccat ttgtttagct acttcatcag
     4501 caaaattttc agactttttc tctattcctt cgccaacttc atatcttaaa acaccggtta
     4561 tttttatatc tgagcctaat ttttttgctg tatcgcttat tactttcttt accgttaaat
     4621 cggcatcctt cacaaatgcc tgctctaaga gacagatttc tttaagctct ttatttagtc
     4681 ttccttcaac aattttttca actatgtttt gcggtttgcc ttcgtttaaa gtctgagccg
     4741 taagtatttc tttttcttgt gctatgaatt cagcatctac atcttccatt gaaatatatt
     4801 ttggactcat tgcagcaacc tgcattgcta tatccttgcc taagacttta agtgcttctt
     4861 tgtcaccttc agattccata gcaacaacaa cggaaatttt tcctccgccg tgaatgtagt
     4921 caactacaac acctttatca acctttactt ctccgaatct tcttagtgac atattttcac
     4981 caatttttgc tattttatct gttaaagctt cctgaactgt tttaccgtca tttagtacaa
     5041 cagcttttaa agcttccaaa tcttttggtt cattgtcaag aacagcctta gctgtttctt
     5101 caacaaagct tttgaattct tcatttttag caacgaaatc tgtttctgag ttcacttcaa
     5161 gaacgacacc ctttttctca tcagctgaaa ttagggctgt agtaagacct tccgatgcaa
     5221 ttctgcttga tttttttgca gcttgtaaaa gtcctttttc ccttaagtat acaattgcct
     5281 tttcaatatc accgtttgtt tcttttaaag cttttttaca gtccatcata cctgcatttg
     5341 ttttttctct tagttctttt accattgaag ctgtaatttc catcttatcc tccataattt
     5401 aaaaagaagg tgtaaggaaa taattcctaa ccccttactt tgttgtttta ttattctaat
     5461 tcttcttcta cttcttccaa ttcttctaac tcttcaagat cttctaattc ttcggttact
     5521 tcgtcttctg tatcttcgtg agtcatctga acgccttgtt tcccttcaat tactgcatct
     5581 gctatagttg ctgtcaacag ttttacggct ctgatagcat catcattgcc cggaataaca
     5641 taatctactt catcagggtc acaatttgtg tcaactattg ctactacagg aattcctaag
     5701 attgctgctt cctgaacagc tattctttct tttctcgggt caactacaaa caaagcaccc
     5761 ggcagtttat ccatattttt tattccgcct aagaattttt caagtctttc ggcttcattt
     5821 cttaatttga aaacttcttt tttggttaaa aggtcgaatg taccatcctc ttccattgtt
     5881 ttcaattcat taagcctgtc aattcttttt ctgatggttg agtagttagt aagcattcca
     5941 ccaagccatc tttggcttac ataatgcatt ccgcatctta tagcttcaga ctttgttgat
     6001 tcctgagctt gtttttttgt tccgacaaat agtatttcgt cgccgtttga tacaacttcc
     6061 tttataaagt tgtaagcttc atctacttta tcgcttgttt tctgtaaatc gataatatag
     6121 attccatttc tttccgtgaa aatatactct gccattttag gattccatct tcttgtttga
     6181 tgtccaaaat gaaccccggc ctcgagcaat tttttcatac tgataattgc cattattttt
     6241 ttcctccttt tgttttacct ccagaaatgt catatcatga aaaagaccgt ttccggcaca
     6301 tctctaacat gatcaaaatc tgtgtgtaat tatgattagt atatcatagc caattttatt
     6361 tggcaatatt taattacaaa aatgtgcata ttataagcta tttttcaata atattattcg
     6421 ctttttttac attttttatt tatgcatgtt tgcttattgt tttttaaaac catcatttcg
     6481 ccgcattcgg gacatttttt gtcggtcggc tcataccatg aaatatattt acaatcagga
     6541 taacctgtgc atccgtaaaa tctccgtcct gcttttgaaa acttgattgc aatttgcttg
     6601 ccgcatcgcg gacagggagc atcaatagtt ttaataatcg gttttgtatt tttacatttg
     6661 ggataattgg agcatgccaa aaattctccg aactttcctt tcctcttaag cattttagag
     6721 ccgcattttt cgcatgtttc atctacttcc tcaggcggtt ctttgccgtt taggggcttt
     6781 gtattcttac attcgggata attgggacat gcaagaaact ttccgaatct tccgctttta
     6841 ataaccatga atgcgccgca gttgtcacat ttgacatcac ttacttcatc tttgatttca
     6901 atttctttca tttcattttc cgctacatca agctcttttt taaagctttc gtaaaaattt
     6961 gaaattacgt tgcgccaatt aatatttccg cttgcaacat catctaagtg gttttctaaa
     7021 tctgcggtaa atttttcatt cacaattttc gggaagtatt tctccatcat atcggttact
     7081 aaaaaaccca tatctgttgg aaacaaatat cctttttctt tagccacata atccctgctt
     7141 gttatagttg taatagtagg tgcatatgtg cttggacgcc ctattccctt ttcctccaaa
     7201 tcctttatga gacttgcttc ggtataacga gcgggaggtt gggtaaaatg ctgctcaggt
     7261 attacttttg agatatctaa tacttcatta atttttactt cgggaaacat tttttcgtct
     7321 ttttcatcag catatccgta tacagctcta aaaccgtcaa aaacaagaac cttcccgttg
     7381 ctttcaaaaa tgttgtcatt gttattaaat aatactttag taacatcata ttccgcatct
     7441 gccatctgac aggcaagagc ccttttccat atcagactat acaacttata ttggtccgta
     7501 gtgaggctgt ctttaatttt ttccggtgtt cgaagcaatg aagtaggcct tatagcttca
     7561 tgtgcatcct gcaccttgct ttcgtttttc tttgcctttt tatatactct gaatttatga
     7621 tatttttctc cgtaatttga taaaataaat tgctttgcat tttcaacagc ttcatcagac
     7681 aatctgaatg aatctgttcg tatataagta accaagccca cattcccttc acctttgact
     7741 tttactcctt
//
LOCUS       DAOSHP010000079         7653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8269_length_7653_cov_8.308107, whole genome shotgun sequence.
ACCESSION   DAOSHP010000079 DAOSHP010000000
VERSION     DAOSHP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8269_length_7653_cov_8.308107"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1205)
                     /locus_tag="PLB33_07555"
     CDS             complement(15..1205)
                     /locus_tag="PLB33_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOG63417.1"
                     /translation="MTDFIIKKFIKDYENVNEQRVRESYGITASITGIILNTLLSIGK
                     ILTGIIFNSISVTADGVNNLSDGASSVITLIGFKISSKPADKDHPFGHARMEYLTGLI
                     LGIAVILVGVELIKSSFDKIMNPTKTIFSIEMIVVLIVSVLIKLWISLFYKKLGDKIS
                     SATLKASSTDSRNDVISTIVVILSLLVSNATGYEVDGYVGILVALFILYSGYDILRDI
                     LNPLLGEMPDREFIESIENKILSYDGIVNIHDLVVHNYGPNRYFASVHAEVDAKEDIM
                     KSHDLIDNIERDFAREFDISLVIHLDPVITDDEEINELRSMTDKIVKSIDERLTMHDF
                     RVVKGETHTNLIFDVVVPVDYDIKSSKLVSMIEKEIRNKDETYFAVVTVDKNYVSTYM
                     NDLK"
     gene            complement(1420..>1857)
                     /locus_tag="PLB33_07560"
     CDS             complement(1420..>1857)
                     /locus_tag="PLB33_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63418.1"
                     /translation="REKSERREESERSEKSEKPNKYEKPNEKDRKKNVIEVIEVTKKE
                     VDEAKKLKVTPGKLTLIKKLQEAANEVHRGYEIDQDEWRHESVQEINAKISEYREELR
                     QKQKETENKNHNKKDTSYKSYKNENKDDEDSHNSNDSKERWKH"
     assembly_gap    1858..1957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1958..2606)
                     /locus_tag="PLB33_07565"
     CDS             complement(<1958..2606)
                     /locus_tag="PLB33_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HOG63419.1"
                     /translation="MKAIIVEIKGRHAAVLTDDGVVSKIKNRNYCIGQEIVIKNNRNK
                     LIRMTAAAAAAIMLFVTPAWAYLTPYSYVSIDVNPSFEFLINRFDRVLTVRGFNDDGR
                     ELSKDINIDGLKNKEVQNAVKSVLNELKNKGYIIEGQEGGVIIATSSKSEAKKDVLFE
                     KLRTAVNEEEAPEQTKDNEIKTKIEEPKEPESTEKLEEKEESAAQQTETIEKTEEL"
     gene            complement(2587..3318)
                     /gene="sigI"
                     /locus_tag="PLB33_07570"
     CDS             complement(2587..3318)
                     /gene="sigI"
                     /locus_tag="PLB33_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HOG63420.1"
                     /translation="MSDLDLLAVEAKTNTDLMNNLIIQYENFILKCASSTTRSYISKS
                     DDEWSIALSAFTEAIKNYSADKGSFLNFAELVIRRRMIDFLRSKSRYAPEVLVNPSLF
                     NTDSQDEEEYSLQSEIAKKVSIVENNSIKMEIALINEIFSDYGFSFMDLTECSPKSHK
                     TKKSCAKVVAYLIKNPILTREIKIKKQLPLNIIEKYTNVPRKLLERHRKYIIAALEIM
                     SGDFPNLAGYMEYIRQELKNESNNS"
     gene            complement(3469..3708)
                     /locus_tag="PLB33_07575"
     CDS             complement(3469..3708)
                     /locus_tag="PLB33_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63421.1"
                     /translation="MKILNEPIKVMAVFNTNGKIEPVKFRLDDKVVRIEKIIKTYEEN
                     IVGNTRIVFVCLHKGKDIFELKYEVASKTWYLFKK"
     gene            complement(3719..4960)
                     /locus_tag="PLB33_07580"
     CDS             complement(3719..4960)
                     /locus_tag="PLB33_07580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOG63422.1"
                     /translation="MLGHIVMHIDVNSAFLSWTAAYEKQMGIERDVRTVPSVIGGNEQ
                     SRHGIVLAKSSPAKKFKIQTGMSLMEARQMCPNLLVIPPNYSVYVRASKALREYLKKY
                     TPDVEVFSIDECFLRFTDINREQAVKLAHKIKDGVREKFGYTVNVGISENKLLAKQAG
                     DLEKPDKCHTMFIEEIKEKLWPLPVEELFMVGRRTKPKLNRWGIYTIGELANADYKLI
                     STMLKSHGRLIYNYAWGRDISIFKERAPIKSVGNSSTLRFDVTDRETAHVVLLSLTEM
                     TAWRLREANMNCRVVSISIKDKDFGFKIKQRKLMYFTDCTRDIYMNACSLFDELWDKK
                     PIRALGVHVSDLEFSEVKQINFFQDKLSLKNEILDKAVDKIRAKYGDNSVIRGVFANS
                     DLKPILGGYPDDEYPGMSSIL"
     assembly_gap    5147..5246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5297..6076)
                     /locus_tag="PLB33_07585"
     CDS             complement(5297..6076)
                     /locus_tag="PLB33_07585"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOG63423.1"
                     /translation="MVLVVDIGNTNIVIGVYDGNELVGNWRLVTRDEKTSDEYGISIL
                     SLLRYNDINHKNINSAIVSSVVPNVMHSFENALKKFFHIDPIIVGPGVKTGITIITDN
                     PREVGAGRIVNIVAAHELYGNGDTIMAIDFGTATTYDIVNEKGEFRYGITSPGIQISA
                     DALWQRTAQLPKIEIKKPDSILGKNTVMSMQAGLIYGYIGQVEYIIKKVKEELHDENI
                     KVVATGGLGRLIYDGTSEIDIFDPHLVFKGLKIIHDKNYKK"
     gene            complement(6103..6633)
                     /locus_tag="PLB33_07590"
     CDS             complement(6103..6633)
                     /locus_tag="PLB33_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOG63424.1"
                     /translation="MREKEVKVLNIDKEAIEKKLVDMGAVLVKDENQINYRFDTNDYF
                     LKKTYNGYLRIRIIKDNLSGETKSTMTFKRSIKRDSLRVNEEINTDISDWKSTAKIIE
                     LLGYEQKRPGYKHRRSYMYENILFEIDTWDEQTYTKPYLEIEVTSDEELEKAIVLLNL
                     DRSQITTKPIDELRKD"
     gene            6858..7244
                     /locus_tag="PLB33_07595"
     CDS             6858..7244
                     /locus_tag="PLB33_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOG63425.1"
                     /translation="MCNKGKFFICETCGNLVGLVLEGGGELVCCGAPMKELVANTEEA
                     AYEKHIPEVKVEGNKVTVQVGSVLHPMVEEHYIQFIYLVTKEGAQKACLCPGDKPIAE
                     FALVEGDEVVAAYEFCNLHGLWKKEQ"
     gene            complement(7464..>7653)
                     /locus_tag="PLB33_07600"
     CDS             complement(7464..>7653)
                     /locus_tag="PLB33_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63426.1"
                     /translation="SGIAKWYKPEDLVGKKVVMIYNLEPVTLRGVESKGMILSAEKGK
                     MLSVVSTLEDMPSGATIS"
BASE COUNT         2154 a         1462 c         1051 g         2786 t
ORIGIN      
        1 ccgtcccctt gtcattattt taaatcattc atatatgttg aaacataatt tttgtcaact
       61 gtgacaacgg cgaaatatgt ttcatcttta tttcgtattt ccttttctat catactcact
      121 agttttgatg attttatatc atagtccacc ggtacaacca catcaaatat taaattggta
      181 tgggtttctc ccttcactac tctaaaatca tgcatggtaa gtctttcatc aatggatttt
      241 actattttat ccgtcatgct tctcaattca tttatttcct catcatccgt aataacgggg
      301 tctaaatgaa ttaccaggct gatatcaaat tctcttgcaa aatccctttc aatgttatcg
      361 attaaatcat gagatttcat tatatcttcc ttcgcatcaa cctctgcatg aactgatgca
      421 aagtatctgt taggtccgta attgtggact acaaggtcat gaatatttac tattccgtca
      481 taggataaaa tcttattctc aattgattca ataaattctc tatcaggcat ttctccaaga
      541 agcggattca atatatctct taatatgtca taacccgaat ataatatgaa taaagccact
      601 aatattccca cataaccatc gacttcatag cctgttgcat tggataccaa taaggataaa
      661 attacaacta ttgttgaaat tacatcattc ctcgaatccg tggaagaagc cttgagggtt
      721 gctgatgaaa ttttatcgcc aagtttttta tagaataaac taatccataa ttttattaaa
      781 acagaaacaa taagtacgac tatcatctca atggaaaata tagtttttgt cgggttcatg
      841 attttatcaa aggatgattt aataagctct acaccgacta aaatcacggc gattcctaat
      901 atgagccctg tcaggtattc cattcttgca tgaccgaaag gatggtcctt gtcggcaggt
      961 ttgcttgaaa ttttaaaacc tataagggta attacagaag atgcaccgtc ggataaatta
     1021 ttgactccgt cagcagtaac agaaatactg ttaaatatta tacctgtcag tattttccct
     1081 atacttaaaa gagtatttaa aataataccc gtaatgcttg ccgttatgcc gtaggattct
     1141 cttacccttt gctcattaac attttcatag tctttaataa attttttaat aatgaaatca
     1201 gtcatcgcaa ttctccaact ataataagct ctctaattag ccttaaataa attatatgca
     1261 ttatataatt atattcttct aaatttaatt catacataat caatatgaac caactgtttt
     1321 cttcttgcgc cgcagaaatt tagcattctt gtcatatgca aatacctgtg ggagtattcc
     1381 cacaggtata aattactata atatagtatt tttgtcaaat taatgtttcc atctttcttt
     1441 tgagtcattg ctgttatgac tgtcttcatc atccttattt tcgtttttgt acgatttata
     1501 tgatgtgtct tttttattgt gatttttatt ttctgtttct ttttgctttt gtcttaattc
     1561 ttccctatat tcacttatct tagcatttat ctcctggact gattcatgac gccattcatc
     1621 ctgatctatt tcatatcctc gatgtacctc atttgctgct tcctgaagtt tctttatcaa
     1681 agtaagttta cccggtgtaa ccttcaattt ctttgcttca tcaacctctt ttttggttac
     1741 ctctattact tcaataacat tcttttttct atccttttca tttggttttt catacttatt
     1801 tggcttttcg gatttttcac ttctttcaga ctcttctcgt ctttcagatt tttctcgnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntaa gttcttcagt tttttcaatt
     1981 gtttctgttt gctgtgctgc agattcttcc ttttcttcta gtttttccgt actttccggt
     2041 tccttgggct cttcaatttt tgttttaatt tcattatctt tcgtctgttc cggtgcttct
     2101 tcctcattaa cggcagttcg aagtttttca aacaacacat ctttttttgc ttctgacttg
     2161 cttgatgtgg caattattac gccgccttct tggccttcta taatatatcc cttatttttt
     2221 aattcattta atacactttt taccgcgttt tgaacttctt tatttttcaa accgtcaata
     2281 tttatatctt ttgacaattc tcttccatca tcattaaaac ccctcacagt cagaaccctg
     2341 tcgaatctgt ttatcaaaaa ttcaaaagat ggatttacat ctatgcttac ataggaataa
     2401 ggagtcaagt atgcccatgc aggtgtaaca aataacatga ttgcagcagc tgccgcagcc
     2461 gtcattctga ttaatttatt tctgttattt tttattacta tttcttgacc tatgcaatag
     2521 tttctgtttt taattttaga aacaacaccg tcatcagtca atactgctgc atgtcttcct
     2581 ttaatttcaa ctattattgc tttcattttt caactcctga cgaatatatt ccatataacc
     2641 tgctaaattc ggaaaatccc ccgacatgat ttctaatgct gctataatat attttcgatg
     2701 gcgttccaac aatttacggg gtacatttgt gtatttttct attatattta atggcaattg
     2761 cttcttaatt tttatttctc ttgtcaatat tggatttttt attaaataag ccacaacctt
     2821 agcgcaggac ttcttagttt tgtgagattt tggcgagcat tcggtcaaat ccataaatga
     2881 aaatccataa tcggaaaaaa tttcattgat gagggcaatt tccatcttaa ttgaattatt
     2941 ttcaacaatg gaaacctttt ttgcaatttc ggactgcaag gagtattctt cttcatcttg
     3001 agaatcagta ttaaacaatg aaggatttac taaaacttcc ggcgcatatc ttgacttgct
     3061 gcgcaagaaa tcaatcattc tccttcttat taccaattct gcaaagttta aaaaactgcc
     3121 cttatcggca gaataatttt taattgcttc agtaaaagca gacaaggcta ttgaccactc
     3181 atcatcactt tttgaaatat agcttcgggt agtagatgag gcacatttaa gtatgaaatt
     3241 ttcatattga attattaggt tattcattaa atctgtatta gtcttagctt caacagccaa
     3301 taggtccaaa tcgctcatgt tcttcccctt gtagttacaa ttacaaaaca ttatatcatt
     3361 cagtccataa tattacaaca ttccaaatct tacaaaattc ttaatgggga aacaaaggaa
     3421 gggggacaca ccttcctatt ttggaatagc ccctgccggc aggaagaatt actttttaaa
     3481 aagataccat gtcttgctgg ctacctcata tttaagctca aaaatatcct tccccttatg
     3541 gagacataca aatacaattc ttgtgttccc gacaatattt tcttcataag ttttaattat
     3601 tttttcaatc ctcacaacct tatcatcaag cctgaatttt acaggctcta tttttccgtt
     3661 tgtattaaaa acagccatta cttttatggg ctcatttaaa attttcatag taccacctct
     3721 ataaaatact gctcataccc ggatattcat catccggata cccccctaag ataggtttta
     3781 aatcactgtt ggcaaaaacc cccctgataa cggaattatc gccatactta gctcgtattt
     3841 tatctacagc cttatctaaa atttcatttt tcaaacttaa tttatcctga aaaaaattaa
     3901 tttgtttaac ctctgaaaac tccaagtctg atacatgaac ccccaatgcc cttatcggct
     3961 ttttatccca taattcatca aataaactgc atgcattcat ataaatatcc cttgtacagt
     4021 ctgtaaaata cataagcttt ctttgcttaa tcttaaaacc gaaatccttg tctttaatac
     4081 ttatgcttac aaccctgcaa ttcatatttg cttctcttaa tctccaggcg gtcatttcag
     4141 tcaaactcag caatacaaca tgagcagttt ccctgtctgt aacatcaaat ctaagggtac
     4201 tgctgtttcc tacactttta atcggggccc tttctttaaa aattgaaata tcccttcccc
     4261 aggcataatt gtaaattagt ctgccgtgag atttaagcat tgttgatatt aatttataat
     4321 ctgcattggc aagctctcct attgtataaa taccccatct gtttaatttt ggctttgttc
     4381 ttctaccaac cataaatagc tcttcaacag gcaaaggcca taatttttcc tttatttcct
     4441 caataaacat ggtatgacac ttgtcgggct tttctaaatc tcctgcttgt tttgcaagaa
     4501 gcttattttc agaaattccc acatttacgg tataaccgaa tttttcccga acgccgtctt
     4561 ttattttatg ggcaagttta acagcctgct ctctattgat gtcggtaaat cttaaaaaac
     4621 attcatcaat gctgaaaact tctacatcgg gagtatattt tttcaaatat tccctgagag
     4681 ctttgcttgc tcttacataa acgctgtaat tgggaggtat aaccagtaaa ttaggacaca
     4741 tttgtcttgc ttccatgagg gacattcccg tctgtatctt gaattttttg gcaggacttg
     4801 atttggcaag aacaattcca tgccggcttt gttcgtttcc tcctatgaca gacggcactg
     4861 tccgtacatc tctttctatt cccatttgtt tttcatatgc agccgtccag cttaaaaatg
     4921 ctgaatttac atcaatatgc atcactatat gccccaatat ttcacctcct gcttaccggg
     4981 tgccaggcac cctatgcttc atgattttcg aacatttgtt ctattattat aacacaattt
     5041 taaaatttta aaagcttttt ttattaattt tttttattta tcctaatatt gtatttgcaa
     5101 gggtataatg gggtttacat aaaaaaaatt ctgtaaacta tcacaannnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnggtt tacataaaaa aagatctgta aactattaca
     5281 aatctttatc aatatattat tttttataat ttttatcgtg aattattttt aatcctttaa
     5341 acaccagatg agggtcaaag atatcaattt ctgaggttcc atcatatatt aacctgccaa
     5401 gtcctcctgt agctacaact ttaatatttt catcatgcaa ttcttctttt acttttttaa
     5461 taatatattc tacctgtcct atatacccgt agattaatcc tgcctgcata ctcattaccg
     5521 tatttttgcc aagaattgaa tcaggttttt taatttcaat ttttggaagc tgtgcagttc
     5581 tttgccacaa agcatctgca gaaatctgga ttccgggaga tgtaatgcca tatctaaatt
     5641 ctcccttttc atttacaata tcataagttg ttgctgttcc aaagtctata gccattatgg
     5701 tatcaccgtt gccgtataac tcatgagctg ctacgatatt cacaatcctg cctgctccta
     5761 cctctcgagg attatcggtt attattgtaa ttcccgtttt caccccggga cctacaataa
     5821 tggggtcaat atgaaaaaac ttttttaagg cattttcaaa agaatgcatt acgttaggta
     5881 caaccgagga tacgattgca ctgttaatat ttttatggtt aatatcattg tatctcaata
     5941 aacttaatat agaaattcca tattcatcgg atgttttttc atctctggta accaatctcc
     6001 agtttcccac aagttcatta ccgtcgtaaa ctccgattac tatatttgtg tttccgatat
     6061 caacaactaa taccataatt tctcctccta tattatctat agttaatctt tcctcagctc
     6121 atctatgggt tttgttgtaa tctgacttct gtcaagattt aacaaaacta ttgctttttc
     6181 caattcttca tcggaagtca cttcaatttc aagataaggt ttagtatagg tttgctcatc
     6241 ccatgtatca atttcaaaca atatattttc atacatataa gatcttctgt gtttatagcc
     6301 gggtcttttt tgctcatatc ccaatagctc gattatctta gcagtacttt tccagtcaga
     6361 aatatccgtg ttgatttcct cattaactct taaggagtct ctttttatgc tcctcttaaa
     6421 tgtcatggtg cttttggttt ctcccgataa attatccttt attattctta ttctcaaata
     6481 gccgttgtaa gttttcttta aaaaataatc attcgtatca aatctatagt taatctgatt
     6541 ttcgtccttt acaagaacag ctcccatatc aacaagcttt ttttctattg cttctttgtc
     6601 aatatttaaa acttttactt ccttctctct cattatgtcc tccattaaat ttaggtaatt
     6661 ataccataat tatttagaat aataaacatt tattatcgtt tgcaaaaaat aatattactc
     6721 ttattaatca atatttgatg agagatgtat cataaatcgg tatcaataat taattaaaaa
     6781 aataagctaa ttttgtttta aatatattta gaagggtata tacttaatgt taaagaacaa
     6841 aacttaggag gtattatatg tgtaataaag gaaaattctt tatttgtgaa acttgcggta
     6901 atttagtagg attggtattg gaaggcggag gggaattagt ttgctgcgga gcccctatga
     6961 aggaacttgt tgcaaacact gaggaagctg cttatgaaaa gcatataccc gaagttaagg
     7021 ttgaaggaaa caaggtaact gttcaagttg gaagcgttct tcacccgatg gttgaagagc
     7081 actatattca atttatttac cttgtaacta aggaaggtgc ccagaaagca tgtttgtgtc
     7141 ccggagataa gccgatagct gaatttgctt tggttgaggg agatgaagtt gttgcagctt
     7201 atgaattctg caatctccat ggactttgga agaaagaaca ataaaggaag agggacacac
     7261 ctctctctag ggtaagtctt tggagtaaga ggaatgtccc tgtacttgta tggagacaca
     7321 cctattattg cttagggata atctttgaag taagaggaat gtccctaagt ccccatactt
     7381 gtccccattc aaaaaaggat ttgagagtaa ggtgccttac ttttcaaatc ctttaatttt
     7441 ttgggttagg tcataaaggt atattatgat attgttgctc cgcttggcat atcctccaaa
     7501 gtagatacaa cggaaagcat cttacccttt tctgccgata aaatcatacc tttagattca
     7561 acgcctctca gtgttacggg ttctaaatta taaatcatta caactttttt gccgaccaag
     7621 tcttcaggct tataccattt agcaattcct gat
//
LOCUS       DAOSHP010000080         7583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8375_length_7583_cov_8.596307, whole genome shotgun sequence.
ACCESSION   DAOSHP010000080 DAOSHP010000000
VERSION     DAOSHP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8375_length_7583_cov_8.596307"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLB33_07605"
     CDS             complement(<1..835)
                     /locus_tag="PLB33_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63427.1"
                     /translation="MQQDKVIVMPFLSNRNIENAASKLNVAYKDAEENFPEDNHNDVY
                     LEISNKLSNLISNIHGESQLIELFAQGGKIPDKKYLTNGVNSIRQNCLKITKTINNLI
                     ELRKLEKKQTCLVVNSVNIVEIIDNIVINASKHIKQEIIFDTNIEEKYMSCDINKLQK
                     AILIILSVAVRYSNKKEILVNLNVFEDNINITVSFNNNSKLLNFFIQKMDNLTPDNLD
                     ELSLSFYLCKSLIALHEGSISVEGNSYETMFTIKLPCENTDSINYLFMRDNKNEYLAE
                     QI"
     gene            1181..1465
                     /locus_tag="PLB33_07610"
     CDS             1181..1465
                     /locus_tag="PLB33_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63428.1"
                     /translation="MAFLNKIGKAIGEAAGTASEKAKDFAETTKLKNAISAEEKQINQ
                     YFLEIGKYVFEQERNNPDSPLAEVFSKITDSLRNIEELKRKITEIKLDNN"
     gene            complement(1532..1906)
                     /locus_tag="PLB33_07615"
     CDS             complement(1532..1906)
                     /locus_tag="PLB33_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG63429.1"
                     /translation="MFFIAGIYPKREELDYYEPIMCSCCTKYGRFEAYVEYSVLSLFF
                     IPLFKFNKKYYARTTCCNSLYLITNKEKGLMMERGQGHNVFLKDTDITLIHKGICENT
                     CPNCGFKVSEEHKYCPTCGNKL"
     gene            complement(1916..3040)
                     /locus_tag="PLB33_07620"
     CDS             complement(1916..3040)
                     /locus_tag="PLB33_07620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG63430.1"
                     /translation="MNDKKKYKISLLGEFIDKSIESEYLIDSLRSSSKITSYTALVIG
                     FIMMLFLVNGYIVDDRTPYFLNIILIRMLVVIISVIIFTVAKKIKEYRNFIYLITFYQ
                     AVMVVYYIFVLTQYNPLSYFSVLGLMVITLAIYILPNKIIISQIISIILSILFFIYPI
                     KKIEGLEEYEFYKIIVYQIILLIYCNVNIYWTEFNKRKEFAANKELMDLSNKDPLTGI
                     YNRLKFDDDMNKWISFSERYGNPFSIILFDIDGFKDINDKYGHLAGDSVAIKIAETIS
                     KSIRDTDIFARWGGDEFVLLLPNTDVQEAENMAERMRECISNNLCDPISNITCSFGVA
                     AYEKNDTTQSLLNKADKLLLQAKASGKDRVVSMDSCTAQD"
     gene            complement(3120..3428)
                     /locus_tag="PLB33_07625"
     CDS             complement(3120..3428)
                     /locus_tag="PLB33_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOG63431.1"
                     /translation="MEKVYKYTITNEEIMENIFKDEKILMNHVIVPPGKVFPKHKTDA
                     IVYALIIRGELSVAIENNEIKTYRAGQLVSIPKGVSTELGNRGEETLELFVVKHEYDL
                     "
     gene            complement(3682..4422)
                     /locus_tag="PLB33_07630"
     CDS             complement(3682..4422)
                     /locus_tag="PLB33_07630"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOG63432.1"
                     /translation="MVKNEFSEKIERLTRSSAYLDFCEEVYGYRVYLFNMMDKQQIDF
                     VLGSIPVTAEDTILDLGCGPGSILNLLVKKYGCRGIGIDQLDGDILDRTGKAFEYIIG
                     DIDRICDYSLKPTVTLSIDSIYFSKDLDKMVLQLNSLENNKMYLFYSQYIFDEAVEDK
                     SILNSHKTKIADALNKNNIFFKTIDFSENECLLYENSIRALQKYKKKFEDEGNLDLYE
                     QILKEDTTGIKLYDKNLAARYLYIIDGM"
     gene            complement(4545..5006)
                     /locus_tag="PLB33_07635"
     CDS             complement(4545..5006)
                     /locus_tag="PLB33_07635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63433.1"
                     /translation="MIVRMAKLSDIKDIYDLICDMENTKFDYEKFQEVFKKYLEDDRF
                     FCFVAEHCKTVIGCLNLRIEYQLHHTAKIAEILELAVMDKYRSKGVGRELFEKASQIA
                     KDSECLQIEVCCNQLRIRAHNFYEKQGMSKFHYKFTMNLHNEQIKENRLGI"
     gene            complement(5055..6506)
                     /locus_tag="PLB33_07640"
     CDS             complement(5055..6506)
                     /locus_tag="PLB33_07640"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOG63434.1"
                     /translation="MKKSWFILTSIIISLLVMLFSGSLQIMRNLRAHAPLDEFKAHMD
                     ERIPALMKLYGIPGCNIALVKDNEVVWTEGYGYADVSNRRALTVDTPMSVQSITKSVT
                     AWGVMRLWEKGLIDVDAPVSQYLKSWQFPLTDYRIEKITVRQLLSHTAGMPLGDFTNI
                     YSPGEAMPSNRDVMSGEAVLMREPGTGFSYSNTGYNLLEILIEDVTGQSFSDYIRREV
                     LLPLGMESAVFDIDKAVTPYPPTGYNLKGEPVPVYLYPSKASGGLFATAHDIARFAAS
                     GMQENPVLSIESINRMYKPESYKIGIYGLVFEAYGFGHYIEKLPNGMVSVSHGGQGNG
                     IMTHMQAVPETGDAIVILTNSQRSWPFIAYVLSNWAQWRGFPSVGMGRIIWGHYGFCF
                     VIGMLVSASLLVILSLVSTFYQQKRAGFRLLRVSAASILLGILIWCACQEYLFITSVF
                     PILSVWLGGAAFVFSIVLLLSVVLHQWPSKYKE"
     gene            complement(6466..6603)
                     /locus_tag="PLB33_07645"
                     /pseudo
     CDS             complement(6466..6603)
                     /locus_tag="PLB33_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
     gene            complement(6579..7454)
                     /locus_tag="PLB33_07650"
     CDS             complement(6579..7454)
                     /locus_tag="PLB33_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63435.1"
                     /translation="MEKLLGREAEIYFSHSKEKPDHLEITGLENVSDIITIDQSPIGR
                     TSRSNAATYTDVFFYIRDLYASLDESKKRNLLPKHFSFNVPGGRCEKCEGSGELTIAM
                     HFLPDVQVKCPVCRGRRFQKSILEVKYKRYSISDVLQLTIAEAANVFCDMPPIMDRLN
                     VLQKVGLGYLSLGQSATTLSGGEAQRIKLAKELGKRTSGHTLYLLDEPTTGLHPHDVK
                     KLVELLNELVGLGNTVICVEHNLDVIAMSDWVVDFGPEGGDAGGEIIVQGTPETVVKT
                     ARSKTGACLSAVMNR"
BASE COUNT         2347 a         1584 c         1174 g         2478 t
ORIGIN      
        1 ctatttgctc agccaaatat tcatttttat tgtctcgcat gaacaaataa ttaattgaat
       61 ctgtattttc gcatggaagc tttatagtaa acattgtttc ataagaattt ccttcgacag
      121 aaatacttcc ttcatgcagg gcaattaatg atttacataa ataaaagctc aaagataatt
      181 catctaaatt atccggcgta aggttgtcca ttttttgaat aaagaagttc aataacttac
      241 tattattatt aaatgaaaca gtaatattaa tattatcttc aaaaacattc agattaacta
      301 atatttcctt cttgttggag tacctgacgg ctacggataa aataattaga attgcttttt
      361 gaagcttatt gatatcacat gacatatatt tttcttcaat attagtatca aaaattattt
      421 cttgtttgat atgctttgat gcgttaataa ctatattatc aataatttcg acaatattta
      481 cagagtttac cactaaacaa gtttgtttct tttcaagttt tctcaattca atcaaattgt
      541 ttatagtctt tgtaatcttt agacaatttt gtcgtattga attaacgcca ttcgtcaaat
      601 attttttgtc cggaattttt ccaccttgtg caaaaagctc aataagctga ctttctccat
      661 gaatattact tataagattg cttaatttat ttgaaatttc caaatataca tcattatgat
      721 tatcttcagg aaaattttcc tctgcatcct tatatgctac attcaatttg cttgctgcat
      781 tttcgatatt tctgttgctt aaaaaaggca ttacaattac tttgtcttgt tgcaatatta
      841 tcacctcccc ccgctattaa ttaccataca tattatttag atttttatca taaaaaatat
      901 aataaaatca aaaaaggtca caaacgtaat cattataaat tgctaaatcg taaaaatgtg
      961 tcgaaatttg gcgctaataa aaaatatttt tgacgtgact taatccttgc tttgtcgtat
     1021 tttgcaacaa cactacaagt tatctgttaa atcaccctca gggtgattgc aaataattca
     1081 cattagtata aaataagtat ataaagtttt catcataaat gtttaaaaaa agaaatataa
     1141 ggtatataaa taacaatata tttgattgga ggggttaacc atggcattct taaacaaaat
     1201 aggcaaggca ataggtgaag ctgccggaac ggcttctgaa aaggcaaaag attttgctga
     1261 aacaaccaaa ctcaaaaatg caatttcagc tgaggaaaaa caaatcaacc aatatttttt
     1321 ggaaatcgga aaatatgttt ttgaacagga aagaaataat ccggatagtc cgttagcgga
     1381 agttttcagc aaaattaccg attccttaag aaatattgag gaattaaaga gaaaaattac
     1441 tgaaataaag cttgataaca attgattgaa aggagcggta ttgatacaat actgctccta
     1501 tattaaaatc aatagatgtt gttttccctt tttataattt attcccgcag gttgggcaat
     1561 atttatgctc ttcgcttact ttaaagccgc agttggggca tgtattttca catattcctt
     1621 tatggattag cgttatgtct gtatctttca aaaaaacatt gtgtccttgt cctctttcca
     1681 tcattagtcc tttttctttg tttgttatta aatacaagct gttgcagcaa gttgttcttg
     1741 cataatactt tttattaaat ttaaacaatg gtataaaaaa taaacttaat acactatatt
     1801 ccacatatgc ttcaaatcta ccgtattttg tgcagcagct gcacattatc ggttcataat
     1861 aatctaattc ttctcttttt ggatatatac ctgcaataaa aaacataaag acctcctaat
     1921 cctgagcagt acagctatcc atactcacga ctctatcctt cccgcttgcc ttagcttgta
     1981 aaagtaattt atctgcttta ttaagcaatg attgagtcgt atcatttttt tcataagctg
     2041 ctacaccaaa actgcaagta atattactta tcgggtcgca caagttatta ctgatacatt
     2101 ctctcattcg ttcagccata ttttctgctt cctgaacatc tgtatttggc agaagaagca
     2161 caaattcatc cccgccccat ctggcaaaaa tatctgtatc cctaatcgat ttactaattg
     2221 tttcagcaat ttttattgct acgctgtcac ctgccaaatg cccatattta tcatttatat
     2281 ctttaaaacc atctatatca aataatatta tagaaaaggg attgccatac ctttccgaaa
     2341 aacttatcca tttattcata tcatcatcaa actttaacct gttataaatg ccggttaaag
     2401 gatccttatt agataaatcc atcagttctt tgttggctgc gaattccttt cttttattaa
     2461 attcggtcca ataaatattt acgttgcaat agataagcaa tataatttga tatacaataa
     2521 tcttataaaa ttcatattct tcaagaccct ctatcttttt aatagggtat ataaaaaata
     2581 aaatgcttaa aataatagaa ataatttgtg aaattataat tttattagga agaatataaa
     2641 ttgctaatgt tatgaccatt aaacccagta cgctgaaata gctgagcgga ttgtattgtg
     2701 tcaatacaaa aatgtagtaa acaaccataa ctgcctgata aaaagttatt aaataaataa
     2761 aatttctata ttcttttatt ttctttgcaa cagtaaaaat tatcactgaa ataatgacga
     2821 ctagcatcct tataagtata atattcaaaa aatagggagt tctatcatca acaatataac
     2881 cgttaacaag gaacagcatc attataaaac caataacaag tgcggtatac gatgtaattt
     2941 tagatgaact tcttaaacta tcgattagat attcactttc aatagacttg tctataaatt
     3001 caccaagcaa agatatttta tacttcttct tatcattcat acacatacaa ccccaaaacc
     3061 aatattaata agtcctttat actactgctc ataatactcc gaccaggcaa gagtttcatt
     3121 taaaggtcat attcatgttt aacaacaaat aattctaaag tttcttcccc tctattgcct
     3181 aattcagtac tgacaccttt aggaatactg actaactgtc ctgctctata ggtcttaatc
     3241 tcattatttt ctattgctac gctcaactct cctcgaataa tcagtgcata tacaatcgca
     3301 tcagttttat gctttggaaa cactttaccg ggtggaacaa taacatgatt cataagaatt
     3361 ttctcatctt taaaaatatt ctccattatt tcttcgttcg ttatcgtata cttataaacc
     3421 ttttccattt attcctccta atccatagaa ggtttacgct atagtgaata ctaaccttaa
     3481 accttttcat atattatacc acttatcatt aagaaaactc cacacctttt tttgaaggac
     3541 ttgacagacg atcaagttag gtagggattt tagtttatac ttttcatgaa atcagcaagc
     3601 aagaaaattt gttactttct tctaggtgga ggttctacac cttcgcggat aaaaatcgcc
     3661 tggtacaaga gataccagcc attacattcc atctattata tacagatatc gagcggcaag
     3721 gtttttatca tacagtttta ttccagtcgt atcttcctta agtatttgct catacaaatc
     3781 taaatttcct tcgtcttcaa atttcttttt atacttttgc agtgctctta tagaattttc
     3841 atataataag cactcgtttt cactaaagtc aatagttttg aaaaaaatat tgtttttgtt
     3901 aagggcatcg gcaattttcg ttttatggct attaaggata ctcttatctt caacagcttc
     3961 atcaaatata tattgagaat aaaacaaata cattttattg ttttctaaac tgtttaattg
     4021 cagaaccatt ttatccaaat ctttgctaaa atagatacta tcaatggata gtgtaactgt
     4081 gggctttaaa ctatagtcac agatcctgtc aatgtcaccg atgatatatt caaatgcttt
     4141 gccggttcta tctaaaatat caccatccag ttgatcaatt ccaattcccc ggcatccgta
     4201 ttttttgact aaaaggttta aaatacttcc gggaccgcac cccaaatcta aaattgtatc
     4261 ttccgccgtt accggaatgg aacctaaaac aaaatctatt tgttgcttat ccatcatgtt
     4321 aaaaagatat actctgtatc catatacctc ttcacaaaag tctaaataag cgctgcttct
     4381 agttaatcgt tcaatttttt cactaaattc attttttacc attgctctgc ccttttttgc
     4441 tgcttcaatc ctgccggatt atttctcgtc tgctatgtgc atcaagacaa ctgtcttaac
     4501 gtcaagtttt taaatgcgta ttaacttaat aatcaagata gttttcatat tcctaatctg
     4561 ttctctttaa tttgttcatt atgtaaattc atagtaaatt tataatgaaa tttagacatg
     4621 ccttgtttct cataaaagtt atgtgctctt attcttaatt gattgcaaca tacctcaatt
     4681 tgaaggcact cactgtcttt tgcaatttgg gacgctttct caaaaagttc cctccctact
     4741 cctttagaac ggtatttatc cattactgcc aattctaaaa tttctgcaat tttcgcagtg
     4801 tgatgaagtt gatactctat ccgcagattt aaacaaccaa tgacagtttt gcaatgttcc
     4861 gccacaaaac agaaaaacct gtcatcttca agatattttt taaagacctc ttgaaatttt
     4921 tcataatcaa attttgtatt ttccatatca caaattaaat cataaatatc tttaatatca
     4981 gacaattttg ccattcttac aatcattttt ttaccctcct ttattcctcc cgaccactcg
     5041 ccgagctcat atgctcactc cttatacttg cttggccatt ggtgtaacac cacagacaac
     5101 agcagtacta tagagaacac aaatgccgca cccccaagcc atacagacag aatcggaaaa
     5161 accgaagtta taaataagta ttcctggcaa gcgcaccaga taagaattcc aagaagaata
     5221 gatgctgcac taactcgaag cagtctgaac cccgctcgct tttgttgata aaaggtcgag
     5281 accaggctca atatcaccag caggcttgca gagaccaaca taccgatcac gaaacaaaat
     5341 ccataatgcc cccagataat ccttcccatg ccgacagaag gaaaccctcg ccactgggcc
     5401 cagttgctta aaacataggc gatgaaaggc cagctacgtt ggctgtttgt aagaatcacg
     5461 atggcgtcgc ctgtctccgg cacagcttgc atatgcgtca tgatgccgtt gccctgaccg
     5521 ccgtgagaaa ctgagaccat accgtttggc agcttttcga tatagtgacc gaatccatat
     5581 gcctcaaata ccaaaccgta aatgccgatt ttatagctct caggtttata catccgatta
     5641 atgctctcga tacttagaac aggattttct tgcatccccg acgccgcaaa gcgggcaata
     5701 tcgtgagccg tggcaaaaag accgcctgaa gcctttgagg gatagagata gaccggaacc
     5761 ggctcccctt ttaggttata tcccgtaggt gggtaaggcg tcaccgcctt gtctatatca
     5821 aagacagcac tttccatgcc aagcgggagc aaaacttccc tgcgtatata atctgaaaag
     5881 ctttgtccgg tgacatcctc gattaatatt tctaataggt tatagcctgt gttagagtac
     5941 gaaaatcctg tcccgggttc gcgcatcagc accgcttccc cactcatcac atcccggttg
     6001 gagggcatcg cctcgccggg agaatagatg ttggtgaaat cccccaatgg catccccgca
     6061 gtgtgactta aaagctgccg tacagtaatt ttttctatcc ggtagtcagt caacggaaat
     6121 tgccagcttt ttagatactg cgacaccgga gcatccacgt cgatgaggcc tttctcccaa
     6181 agccgcataa ccccccaagc cgtaacagat tttgtaatgg attgaacact catgggggtg
     6241 tcaaccgtca gagctcttct atttgacaca tccgcatacc cataaccctc agtccatacg
     6301 acttcattgt ccttaacaag cgcgatgttg cagcccggaa ttccatacag cttcatcaga
     6361 gcaggaattc gttcatccat atgtgcctta aattcgtcaa gcggcgcatg ggcacgcaaa
     6421 tttcgcatga tttgcagact gccggaaaac agcatgacaa gtaagctaat tattatggat
     6481 gtaaggataa accatgattt tttcatacct aaacctcaaa tcattaaaag ccttattcaa
     6541 ttcttcctta gtattcatga caatagggcc gccccaagct acctgttcat aactgcgctt
     6601 agacatgcac ctgttttaga gcgggctgtt tttaccaccg tttccggcgt accctgcaca
     6661 ataatctcgc caccggcatc cccaccctcc ggtccgaagt caaccaccca atcggacatt
     6721 gcaatgacat cgagattgtg ttctacgcaa atcacagtgt tgccaagccc gaccaattca
     6781 ttcaatagct caaccagctt tttgacgtcg tggggatgaa gccccgtggt cggttcatcc
     6841 aaaagataca gcgtatgccc gcttgtacgc tttccgagtt cctttgccag ctttatgcgc
     6901 tgcgcttcgc cgcccgataa ggtcgtggcg gactgtccaa ggcttaaata tccaagcccg
     6961 actttctgca aaacgttcag acgatccata atgggtggca tgtcgcagaa cacattagcg
     7021 gcctcagcaa tggtcaattg taaaacgtcg gaaatgctgt atcttttgta ctttacctct
     7081 aaaatgcttt tctggaaccg tcgcccgcga cagacagggc atttaacctg aacatcaggc
     7141 aggaaatgca tagctatggt aagctcaccg ctcccctcgc atttttcaca tctgcctccc
     7201 ggaacattaa aggagaaatg ttttggcagc aaatttcttt ttttggattc atccaaagaa
     7261 gcatataaat ctctgatgta gaaaaagaca tccgtatagg tcgcggcgtt agaacggctt
     7321 gttctgccga tgggcgattg gtcgatggtt ataatatcgc tgacattctc caatcccgta
     7381 atttccaggt ggtcgggctt ttctttggaa tggctgaaat aaatctctgc ctctctgcca
     7441 agtagttttt ccaattttct gttcgcgaga atatgtcgat acaatgtagg atgccgttca
     7501 gtgtacaata aagattctgt atcaatccgg cttctcttta gcggtatatg ttattttctg
     7561 catacggcgc aaacgatagg act
//
LOCUS       DAOSHP010000081         7567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8404_length_7567_cov_9.197684, whole genome shotgun sequence.
ACCESSION   DAOSHP010000081 DAOSHP010000000
VERSION     DAOSHP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8404_length_7567_cov_9.197684"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLB33_07655"
     CDS             complement(<1..129)
                     /locus_tag="PLB33_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOG63436.1"
                     /translation="MMKALYSGVSGMRSHQTKMDVIGNNIANVNTYGYKTQRTTFRD"
     gene            complement(208..582)
                     /locus_tag="PLB33_07660"
     CDS             complement(208..582)
                     /locus_tag="PLB33_07660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOG63437.1"
                     /translation="MTDINFIKKISSLNPNIQPNYNAHRNDSNNFKELLSEKLKNEVS
                     FSKHANERIQQRNIDVSQEVTEKLNEAAVQAKDKGLKNVLVMIDNQAFILSTMNNKVI
                     TAVNSDDLKENIFTNIDGAVIK"
     gene            complement(595..1164)
                     /locus_tag="PLB33_07665"
     CDS             complement(595..1164)
                     /locus_tag="PLB33_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOG63438.1"
                     /translation="MEINAYNSSYKVNNINTANDLKIQKNNEGLNMQDFLNLLVAQIT
                     NQDAMNPMDNTEFIAQMAQFSTLQAMTDLTEMALQGQATSLIGKNVVVADYNGNGGLT
                     IDEGIVQRVTLHGGKTKLYVNDMEYEYSNVMEIKEAEKAEENSLTEMLGNISEKIDNL
                     NNTNNSNISPTDEIPPIDEEEALPYDETQ"
     gene            complement(1154..2233)
                     /locus_tag="PLB33_07670"
     CDS             complement(1154..2233)
                     /locus_tag="PLB33_07670"
                     /inference="COORDINATES: protein motif:HMM:NF021032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOG63439.1"
                     /translation="MNVNSLTISQLPGKVIRSDYSNSVNKNMFENMLNNAMAENQNMM
                     ELLGKNFGSILAANSIEIEDYSIEHENKDETQKQNFYENIYGIADIFFFRPNAAIEEV
                     TAENKITNVDYNFIDAKIQNGTDITAGSALKNLVSEIEIQRDLGISNDIKSSNEVQSM
                     KTILELGKKQKNEPAYLEMPDCIDLKNKIITISDGSTEIKSQVLSQVKDKIIIMTENS
                     QDSNGIKTITMELRPQALGKVDIKMSYENNKLTVEIKASNEETQKILSSNTDELKELL
                     NKTSETDVKIIVKPYEPKNQNILNYQDNRQGSENFYQDNEQNNQGRQRNKYYYDNDIK
                     TKEEDVFSELIDLNYSRIKEGMHGN"
     gene            complement(2424..2876)
                     /locus_tag="PLB33_07675"
     CDS             complement(2424..2876)
                     /locus_tag="PLB33_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63440.1"
                     /translation="MKKFVFSLQKVLELKNQLLENMKIELTNLNRECEKIEIFIKCLK
                     IKFAEIENEYVEKSYKSISAGEMSYYKMLSESILVQIENKEIEKAIVLKKIADKRQEI
                     ISMNTEISSLEKLKEKELEKHSKECLKKEELFIEEFVSNKTMTNEYAI"
     gene            complement(2919..4226)
                     /gene="fliI"
                     /locus_tag="PLB33_07680"
     CDS             complement(2919..4226)
                     /gene="fliI"
                     /locus_tag="PLB33_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOG63441.1"
                     /translation="MLVNEKFNDIRETLIKEDFYTHIGKVKRISGLMIEAHGGKYKIG
                     EICEIKTASNKTIRAEVVGFNDDRVLLMPYEDIKGIGLGNLVVSTNNKLKIPVGDFLI
                     GRIIDATGQAIDGGKKFISQEYCYVDNDYINPLSRPRISEPLSFGVKAIDGLLTIGKG
                     QRMGIFSGSGVGKSTLLGMVAKNVKADINVIALVGERGREVREFIEKDLGEEGLRRSV
                     LVIATSDQPAMLRVKCALTATTIAEYFKDKGKDVLLMMDSLTRFAMAQREIGLAAGEP
                     PVSRGYTPSIYAELPKLLERSGNFQEGSITGVYTVLVEGDDTNEPISDTVRGILDGHI
                     VLNRKLANSNHYPAIDINASISRLMDDIATEEHNNKAKKLRDILAEYYSNYDLISIGA
                     YKKGSNVKLDEAISKIEEVNNFLKQGVNEKYNFDEIINLMDKI"
     gene            complement(4216..4971)
                     /locus_tag="PLB33_07685"
     CDS             complement(4216..4971)
                     /locus_tag="PLB33_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOG63442.1"
                     /translation="MSNIIKAEYVICDIDKQKNNHIHRQVSTYREDLFRIYNQREILL
                     KEAEEKAAEIVSTAIENAQLEITECKKNAYEDGYNAGMEAGRNKGYKEGYDTGKSKIS
                     EILLEQNKEKLNEIAEMIKTIESEKLKIISRYEEELIKLSIEIAEKIIRHEIDTKDDV
                     VSGIIKNAIKDYRNVDWIKIYLSDKDDAEIIQADKELISELEKISKDIKIEVMDELQK
                     GGAIIETADGIVEASIDTQLKNLKEMVLNKNAG"
     gene            complement(4964..5968)
                     /gene="fliG"
                     /locus_tag="PLB33_07690"
     CDS             complement(4964..5968)
                     /gene="fliG"
                     /locus_tag="PLB33_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOG63443.1"
                     /translation="MAERLTGKQKAAAIIISLGAEDASRIYKFLKEDEIEQLTYEISR
                     LQHLSPQTMESTLKDFYELCLTQKVITEGGLEYAKNVLEKAFGTQVATSLLERVTKTL
                     RNKSFDFIRQADYKNLLTIIQNEHPQTIALILSYARADQAAAVIAELPKEKRIDVVER
                     IAKMDRTSPEIVKYVEKELLKKFNSMVSIDYTEIGGINYVADIMNNMDRGNEKYIFDE
                     LNKKDAKLSDEIRKKMFIFEDIVTLDSLSIQRFLREVDSKDLVLSIKGSNKDVSDVLF
                     NNMSTKMADTIRSELEYTHNVRLRDVEEAQQRIVSVIRRLEEEGEVIIAKSGKDDIIV
                     "
     gene            complement(5970..7532)
                     /gene="fliF"
                     /locus_tag="PLB33_07695"
     CDS             complement(5970..7532)
                     /gene="fliF"
                     /locus_tag="PLB33_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOG63444.1"
                     /translation="MSEQLKNIWKNVTEFWGKLNKRKKSLIIGGLLVLVIGALIITFI
                     LNNKDYVVLFRNMDEAESVEVMKQLQESKIDYKYQTDGTILIPKEQESLLRMQLAQAG
                     HPRTGINYDIFTENIDFMTTDYEKRQYEIFQLQERLQSSIETIDGVKEAIVTINLPQD
                     KSFAWETDKKESSASVKINLQNGYSLSPSQTNGIMQLLVKSVKGLKEENVAIIDTNGN
                     SLNISNEMLQTNTIKLKLSIEREFERETEEDVTEFLSRIYGPENVKVSAKCTINFDKK
                     ISEILQYLPDEETRLGVPGDTHSEKEITGPGETTGGIAGTETNAEVPTYPGVVVSGDD
                     IYFRDSNTINYLVSQLKEQIEHNPGTIEKLTVAVVINKANMSDKEAGEINQLIAFAAG
                     IGTEDIALSNMVFYDLEEPAVPVAATGIFERIQLKELLIYGGIALIAISILLIILILV
                     LRKRKKKKALDNAAITSDTDSSEYSWADIQDGIKVQETEEQAIKKQLKDFTKTSPEIV
                     SQLIRTWLKGDE"
BASE COUNT         1855 a         1508 c         1083 g         3121 t
ORIGIN      
        1 atctctaaaa gttgttcttt gtgtcttgta tccataagta tttacgtttg ctatattgtt
       61 gcctattacg tccatttttg tttggtggct tctcattccg gatacaccgg agtaaagtgc
      121 tttcatcata atttttattc tccttttaat tttctttgct gttagtccgt caagatatca
      181 ggaccttagc ctccttaaaa ggaccggcta ttttattaca gctccatcga tatttgtaaa
      241 aatattttct tttaagtcat cactgttcac tgccgttatt actttgttgt tcattgtgct
      301 tagtataaat gcctgattgt ctatcattac aagaacattt tttaaaccct tgtccttggc
      361 ctgaacagct gcttcattaa gtttttccgt tacttcttgg cttacgtcga tatttctttg
      421 ttgaattctt tcatttgcat gctttgagaa actaacttca ttttttaatt tctcactcag
      481 cagctcttta aagttgttgc tgtcatttct gtgtgcattg taatttggct gaatattggg
      541 atttaagctg cttatttttt ttataaaatt tatgtctgtc atatcatcac ctgcttattg
      601 tgtttcatca tacggcagtg cctcttcttc atcaatcggc ggtatttcat cagtaggcga
      661 aatatttgaa ttattggtat tgttcaaatt atctattttc tccgaaatgt ttcctaacat
      721 ttctgtcaag gaattttcct ccgctttttc agcttcctta atttccatta cattggaata
      781 ctcatattcc atatcattta catacagctt tgtttttccg ccgtgcagcg taactctttg
      841 aactattcct tcatcaattg ttaatccgcc gttaccgtta tagtcggcaa ccacaacatt
      901 tttccctatg agtgatgtag cttgtccctg caatgccatt tccgtcaggt ccgtcatagc
      961 ctgaagcgtt gaaaactgcg ccatctgtgc tataaattcc gtgttgtcca ttggattcat
     1021 agcatcctga ttagtaatct gagctacaag caaattcaaa aaatcctgca tatttaaacc
     1081 ttcattattt ttttgaattt ttaaatcatt tgctgtatta atgttgttta ctttgtagct
     1141 tgagttgtaa gcgttaattt ccatgcatac cctccttaat cctggaataa tttaagtcaa
     1201 taagctctga aaatacatct tcttcttttg tcttaatatc gttatcatag tagtatttgt
     1261 tgcgctgcct gccttgatta ttttgttcat tgtcttgata gaaattttcg ctgccttgcc
     1321 ggttgtcttg ataatttaaa atattctgat tttttggctc ataaggcttc acgatgattt
     1381 ttacatctgt ttctgatgtt ttatttaaca actcctttag ctcatctgta tttgaagata
     1441 gtattttttg agtttcttca tttgatgctt tgatttctac cgttaactta ttattttcat
     1501 agctcatttt aatatctacc ttgcctagag cttgaggtct gagctccatg gtaattgttt
     1561 ttatgccatt tgaatcctga ctgttttctg tcataataat tattttgtct tttacttgag
     1621 atagtacttg tgacttaatt tctgttgagc cgtcactaat agttattatt ttgtttttta
     1681 agtcaataca atccggcatc tctaaatatg ctggttcatt tttttgtttt ttgccaagct
     1741 caagtatagt cttcatactt tgtacttcat ttgagctttt gatgtcattg ctgattccta
     1801 gatctctttg gatttcaatc tctgacacca aatttttaag tgctgagccg gcagtgatat
     1861 cagtgccgtt ttgaatctta gcatcaataa aattataatc cacatttgtt attttatttt
     1921 cggctgtaac ttcttcaatt gctgcattgg gtctgaaaaa gaaaatatct gcaataccat
     1981 atatattttc ataaaaattt tgtttttgtg tttcatcttt gttttcatgc tcaatactat
     2041 aatcttcaat ttcaatactg tttgcagcca gaatgcttcc gaagtttttt cctagcaact
     2101 ccatcatatt ttgattttct gccattgcat tattgagcat attttcaaac atatttttat
     2161 tcacgctgtt tgaataatca cttcttataa ccttaccggg caattggctt atcgttaaac
     2221 tgtttacatt caaattctca cctcctttca atataattca gcagttgggg acacacacga
     2281 ggagtgtccc cgtacttaca ttcatatttc cctcctttct attgatattc aatggtttca
     2341 cctgtagttg ttatcccggg ctcagacttt gagatccttc gctgcgctca ggatgacgcg
     2401 ggctgcgcca tcaggtctcc aaattatata gcatattcat ttgtcatggt tttatttgaa
     2461 acaaattcct ctatgaaaag ttcttccttt tttaaacatt ccttgctgtg tttttctaat
     2521 tccttttctt ttagtttttc taaacttgaa atttctgtat tcatactgat tatttcttgt
     2581 cttttgtctg ctattttctt taatactatt gctttttcaa tttccttatt ttcaatctgt
     2641 actaatatgc tttcactgag cattttataa taagacattt caccagctga tattgattta
     2701 tatgattttt ctacatattc attttctatc tctgcaaact ttatttttaa gcatttaata
     2761 aatatttcaa ttttctcaca ttctcgattt aagtttgtga gttcgatttt catattttcc
     2821 aacagctgat tttttaattc taaaactttt tgaagtgaaa aaacaaattt cttcatttcc
     2881 tgcctcgttt aaaatggtat ttttgtaact agaaagtatc aaattttatc catcaaatta
     2941 attatttcat cgaaattata tttttcattt acaccttgtt ttaaaaagtt atttacttcc
     3001 tcaattttac taattgcttc atccaattta acattgcttc cttttttgta ggcacctatg
     3061 gaaattaaat cataattaga gtagtattca gctaatatat ctctgagttt tttggcctta
     3121 ttgttgtgct cttcggttgc aatatcatcc ataagtcttg atatgcttgc atttatgtcg
     3181 attgccggat aatgatttga attagccaat tttctgttta gtactatatg tccgtcaagt
     3241 attcctctga cggtatccga aataggctca ttggtgtcat ctccttcaac aaggactgta
     3301 taaacacccg taattgaacc ttcttggaag tttccgctcc tttctaacag ctttggcagc
     3361 tcggcatata ttgacggggt ataccctctt gatacaggcg gttcacctgc agccaggcct
     3421 atttctcttt gagccattgc aaatcttgtc agggagtcca tcattaataa aacatcttta
     3481 cctttatctt tgaaatattc tgctatggtt gttgctgtta atgcacattt tactctaagc
     3541 atggcaggtt ggtcggatgt tgctattacc aatacggacc ttcttaatcc ttcttctcct
     3601 aagtcttttt caatgaattc tcgtacttct cttcctcttt ctccaaccaa tgcgatgaca
     3661 tttatatccg cttttacatt ttttgcaacc atcccgagaa gggtgctttt cccaacgccg
     3721 cttcccgaaa atatgcccat acgctgtcct tttccgatgg ttaaaagtcc gtcaattgct
     3781 tttactccaa agcttaaagg ctcgcttatc cgaggtcttg acaaaggatt tatataatca
     3841 ttatcaacgt agcaatattc ctgactaata aattttttgc cgccatcaat agcttgtccc
     3901 gttgcatcta taattctgcc tatcaagaaa tctcccacgg gtatttttag cttgttgttt
     3961 gttgatacaa cgagattccc gagacctatg cccttaatat cctcataagg catcaagagc
     4021 actctgtcat cattgaagcc cactacttct gctctgattg ttttgtttga tgcagttttt
     4081 atttcgcata tttctccgat tttgtacttg ccgccgtgtg cttctatcat caatcccgat
     4141 attcttttga cttttcctat atgtgtatag aaatcttctt ttattaatgt ttctcgaata
     4201 tcattaaatt tttcattaac cagcattttt attaagtacc atttctttaa gattttttaa
     4261 ctgggtatca atacttgctt cgactattcc atctgctgtc tcgattattg caccgccttt
     4321 ttgcaattca tccataactt ctatttttat gtctttagat attttctcaa gttcacttat
     4381 taattcttta tctgcttgaa tgatttctgc atcgtcttta tccgaaagat aaatctttat
     4441 ccaatctaca ttgcggtaat cttttattgc atttttaatt attcccgata caacatcatc
     4501 ttttgtatca atttcatgcc tgataatctt ttctgctatt tctatagaga gtttgattaa
     4561 ttcttcttca tatcttgaaa ttatttttag tttttcggat tctatggttt taatcatttc
     4621 agctatttca tttaattttt ctttgttttg ctctaagagt atttctgata tcttagattt
     4681 tcccgtatca tatccttctt tgtagccttt gtttctgcct gcttccattc ctgcattata
     4741 gccgtcttcg taagcatttt ttttacattc tgttatttcc aattgggcat tttctattgc
     4801 agtactgaca atttcggcag ccttttcttc ggcttctttt agaagaattt ctctttgatt
     4861 ataaattcta aacaaatctt ccctgtaagt actcacttgc ctgtgtatat gattgttttt
     4921 ctgtttgtct atgtcacaaa tcacgtattc ggctttaatt atattagaca ataatatcat
     4981 ccttcccgct cttagcaatt ataacttctc cttcttcctc aaggcgtctg attactgata
     5041 caattctttg ctgagcttct tctacatctc ttaagcgtac attgtgagtg tattcaagtt
     5101 ctgaccttat tgtatctgcc atcttagtgg acatattatt gaataatacg tccgaaacat
     5161 ctttatttga acctttaatt gacaatacca agtctttgct gtcaacttca cgaaggaatc
     5221 tttgaatact caatgaatct aaggttacaa tatcctcgaa tatgaacatc ttctttctga
     5281 tttcatcact tagcttagca tcctttttat tgagttcatc aaatatatac ttttcgtttc
     5341 ctctgtccat attattcata atatcagcaa catagtttat gccgccgatt tccgtataat
     5401 caatagatac cattgaattg aatttcttca ataattcttt ttccacatac tttacgattt
     5461 ccggtgatgt acggtccatt tttgctattc tttctaccac atctattctt ttttcctttg
     5521 gcaattctgc aataactgcc gctgcttggt ctgcccttgc ataagatagt attaaagcta
     5581 ttgtttgagg atgctcgttt tgtataatgg ttaataaatt tttgtaatca gcttgtctaa
     5641 taaaatcaaa cgacttattg cgcagtgtct ttgttactct ttctaaaaga cttgttgcta
     5701 cttgagtgcc aaatgctttt tcaagtacat tttttgcata ttctaaaccg ccttctgtta
     5761 ttactttttg cgtaagacat agctcgtaaa aatctttgag cgttgattcc attgtttggg
     5821 gactaaggtg ctgcagcctg gaaatttcat atgttaattg ctcaatttcg tcttccttta
     5881 aaaatttata aattcttgat gcatcttcgg caccgagaga tataattatt gccgcagctt
     5941 tttgtttacc tgttagtctt tccgccatat tattcatccc cctttaacca ggttctgatt
     6001 agctgtgata cgatttccgg actggttttc gtaaagtctt taagttgttt cttaatcgct
     6061 tgctcctctg tttcttgaac cttgattcca tcttgtatat cagcccatga atactcgctt
     6121 gaatctgtat ctgatgttat ggctgcatta tctaaggcct ttttcttttt tctttttctt
     6181 aacacaagaa ttaatattat taataatatt gaaattgcaa ttaaggctat accgccgtaa
     6241 ataagcagct cttttaattg aatcctttca aagatacctg ttgcagccac cggtactgcc
     6301 ggttcttcta agtcataaaa caccatattg ctcaatgcaa tatcttcggt tccgatacct
     6361 gcagcaaatg ctatgagttg attaatttct cctgcttctt tatcgctcat gtttgctttg
     6421 ttaattacta ctgctaccgt cagcttctca attgttcccg gattgtgctc aatttgctcc
     6481 tttaactggc ttaccaggta attaattgtg tttgaatccc tgaaatatat atcatctccg
     6541 ctgactacca caccgggata agttggtact tctgcattag tttcagttcc tgcaattccc
     6601 cctgttgttt ctccgggacc agtaatttct ttttcgctat gagtatcgcc gggtacaccc
     6661 agtcttgttt cctcgtccgg taaatactgc aatatttcgc tgattttttt gtcaaaattg
     6721 atagtacatt ttgccgaaac cttgacattt tcaggtccgt atattcttga aagaaattct
     6781 gttacatctt cttcagtttc tctttcaaat tctctttcta tactaagttt taattttatt
     6841 gtatttgttt ggagcatttc attgctaata ttcaaactat tcccgttggt atcaattatt
     6901 gcaacatttt cttcctttaa tcctttaacg cttttaacca gcaactgcat tataccgttt
     6961 gtctgggaag gagataggga atatccgttt tgcaaattta ttttaacaga ggcagaagat
     7021 tcctttttat cggtttccca tgcaaaactc ttgtcttgcg gtaaatttat agttacaatt
     7081 gcttccttaa caccgtcaat agtttctatt gagctttgca gtctttcttg taactgaaaa
     7141 atttcatatt gcctcttttc ataatctgtt gtcatgaaat caatattttc ggtaaagata
     7201 tcgtaattta taccggttct gggatggccg gcttgagcta attgcattct taataagctt
     7261 tcttgttctt taggtatcaa tatggttcca tctgtttgat atttatagtc aattttgctt
     7321 tcttgcagct gtttcataac ttcaaccgat tcagcttcat ccatatttcg aaataaaact
     7381 acataatctt tgttattcag tataaaggtt attattagtg caccaattac caatacaaga
     7441 agtccgccta ttataaggct ttttttcctt ttatttagtt ttccccaaaa ctcagtaaca
     7501 tttttccata tgttttttaa ttgttctgac atttatagct cctaagatag tatgagacct
     7561 aaacccc
//
LOCUS       DAOSHP010000082         7534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8454_length_7534_cov_9.717877, whole genome shotgun sequence.
ACCESSION   DAOSHP010000082 DAOSHP010000000
VERSION     DAOSHP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8454_length_7534_cov_9.717877"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLB33_07700"
     CDS             complement(<1..223)
                     /locus_tag="PLB33_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOG63445.1"
                     /translation="MNITKEKAWELLKKYNKDEFHLQHADTVSFVMEYFAKKLGFEDE
                     AEFWGIVGILHDIDFEMYPDEHCVKMQEIL"
     gene            514..1278
                     /locus_tag="PLB33_07705"
     CDS             514..1278
                     /locus_tag="PLB33_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOG63446.1"
                     /translation="MKDITRVQSIDRAVAILDCFSEEKKELKLSEISERLGLNKSTVH
                     GIISTLKYHGFISQDEETQKYKLGIRFVQFGDLVINSMNIRNAAVPVIDAVCEKIEET
                     VHVAMLDGLDVVWIEKRECTKSIKTSTKIGARLPAYTTADGKIIICYQNKDKIKNYLP
                     KRIPQYTNNTITNKGEFIKKLEEMKKNGYAIDNEEYVEGLKCVAAPIFDHDGKVRFSL
                     STTGPAFRMNEERIKELIVIIKEAANEISQRIGYMK"
     gene            1602..2687
                     /locus_tag="PLB33_07710"
     CDS             1602..2687
                     /locus_tag="PLB33_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63447.1"
                     /translation="MKKLLAIVLILAMSLFAFVACTQDQPGETPKNEEPKTEEPKTEE
                     PKEEEPLAEGAVKTGLAVISSVEKSKDAGEKDGLAQTDSVIVAVTVDKDGKIVQTIID
                     TAQTKIEFSKEGKIIPDLNTVFKSKQELKEEYGMGKASSIEKEWYEQANALAEYVKGK
                     TVEEVKAIAVDETGVTTEADLVSSVTIKINPYKEAIEKAVANAENLGADASDKLGLGV
                     ITTIAKSKDATADAEGLAQAYSTYTVATFDADGKVTSSIIDASQANVKFDNTGKITTD
                     LAVAPLTKVELGEDYGMKKASAIGKEWYEQADAFADYVVGKTVEEIKGIAVDESGHAT
                     EEDLVSSVTVGIVDLKTIFEKAYNNSI"
     gene            2761..3807
                     /locus_tag="PLB33_07715"
     CDS             2761..3807
                     /locus_tag="PLB33_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOG63448.1"
                     /translation="MAKKIISVILLILIIISLTGCSDKEPIRYQSEFIMLFDTVTRIV
                     GYSKSKEEFTEYAQIIYDNLKEYHQLYDIYNDYEGINNIKTINDNAGIKPVKVDKRII
                     DLIKFSKDMYEKTNGKTNIAFGAVLRIWHEYRTIGIEDPANAALPPEEELRIASEHTN
                     IEDIIIDEEQSTVFLRDPFMSLDVGAVAKGYATEQVSKIAKESGMNSALLSVGGNVRA
                     INKNVTSNEPWNVGIQNPDTEAEESVIKIVKLDDSSLVTSGDYERFYIVDGEKYNHII
                     NPDTLYPAKYFTAISIICKDSGLADALSTAVFCMPFEEGLEFINSLPEAEAMWIFSDK
                     TIKYSDNFESFIKK"
     gene            3902..5707
                     /locus_tag="PLB33_07720"
     CDS             3902..5707
                     /locus_tag="PLB33_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63449.1"
                     /translation="MKKESELKIIPILSKGFKRFFFISAAATILKVSASFAVPQVIRF
                     TVDHIIDDYPLNYIQLVMNTINNIGGIEFLKENLWLLTFFILVLAVITGVSDYIGKIT
                     LAKGTEGIIKNIRDKLFEHIQKLPYSWHIKNQTGDIIQRSTSDVEVVRNFISTQLVEI
                     FRVIMLVIISLILMFSMNFHLTAAVLVFIPVVVLYSTIFYGILTKKFLVADEAEGKLF
                     TAIQENLTGVRVVRAFGREKHEIEKFTERNNTFSELWVKLGYQLGYYWGIGDLVTGVL
                     LMVIVVYGTIFAINDRITLGEFLAFASYTVMISWPLRSFGRILSELSKAKVSLKRICE
                     ILKEPVEDDSPDSVTPDILKDIEFKNVTFKYDGETEVLKNVSFTIKAGSTFAILGGTG
                     SGKTTLMYLLNRLYDLPDHMGCITIGGVSIKNIKREHLRKNIGMVLQEPFLFSRTIKE
                     NIAIVEPDTSLDEIKKAASIAQIDESIEEFSKGYDTIVGERGVTLSGGQKQRVAIARM
                     LLQKTPIMVFDDSLSAVDADTDAKIRSSLIKSKGSSTIIIISHRITTLMEADTILVLD
                     KGEVSDIGTHEELINRDGIYKDIFNVQMNLSEDIE"
     gene            5704..7527
                     /locus_tag="PLB33_07725"
     CDS             5704..7527
                     /locus_tag="PLB33_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63450.1"
                     /translation="MNYYEEESYSSFNIKPWLKLGRFFKPYTGKLLGIIFFMFITALM
                     DLSIPLFQKYAVDSFIAKNTTEGINGFIALYTAVLVIMAISVNIFIRLAIVVEMHIGM
                     DLRKEIFEHLQNLSLSYYNTTPVGYIMARTLSDTNKIGSMIAWGLVDVFWSFAYVTGA
                     FIAMLLLNVQLTVIIAIVIPVMAVITVYFQNKILLTSRKLRKINSKMTGSFNEGIIGA
                     TTSKTLVIEDKNLVQFKNISSEMKMTAVNLARLNALYIPIIVFFGSVLTALVLAKGGR
                     LAMDKIIQIGTLSAFLSYAISIFEPVQQLARTFAEVISLQANIERVTDLLEKEPLIKD
                     SEEVVKIYGDTINPKKENWEEIIGEIEFKNVSFKYPDGDEEVLQNFNLHIPAGTNVAI
                     VGETGAGKSTLVNLACRFFEPTEGEILIDGKDYRERSQLWLHSNIGYVLQDPHLFSGT
                     IKENIRYGKLDATDEEIMEAAKIVSVDTIINKLEKGYDSHVGEGGDRLSTGEKQLISI
                     ARAILSNPKIFILDEATSSVDTQTEILIQKGIEYLLKGRTSFIIAHRLSTIKKADIIL
                     VVNEGKIIEQGTHNELIAKRGHYYNLYTKQFEEEKTLEVLK"
BASE COUNT         2775 a         1040 c         1469 g         2250 t
ORIGIN      
        1 acagtatctc ctgcattttt acgcaatgtt catcaggata catttcaaag tcaatgtcat
       61 gcaaaattcc tacaattccc caaaattctg cttcatcttc aaaacccagt tttttcgcaa
      121 aatattccat tacaaagctt acagtatctg catgctgcag atgaaattca tccttgttgt
      181 actttttaag caattcccat gctttttctt tagtaatatt cattatatcc cttccttata
      241 atttaatatc ataattttaa cttatttaac ataacatgtc tataacaaaa aattttttat
      301 ttgacaaatt ttcatacaga ttgacaaata atttacatag tgatatagta tcattataaa
      361 ttgtattgta gtatttaaac attgaaatta aaatacttat agcataatat taaaaaaatt
      421 aataaataat tggatactgc acaattcgtt tgttaataaa ttatttatat tgattttttt
      481 aaaatcacat gctataattt tggagtatcg tttatgaaag atataacgag agttcaatcc
      541 attgacaggg cagttgcaat attagattgt ttcagcgaag aaaaaaaaga acttaagctg
      601 tcggaaattt ctgaaagatt aggtttaaac aagagtactg ttcatggaat tatatctact
      661 ttaaagtatc atgggtttat ttctcaagat gaagaaactc aaaaatacaa attaggtata
      721 cggtttgtcc aatttggaga cttagtaatt aattcaatga atatacgaaa tgcagcggta
      781 cctgttattg atgcagtttg cgaaaaaatt gaagaaacag ttcatgtagc aatgcttgac
      841 ggactggatg ttgtttggat agaaaaaaga gaatgcacca aatcaattaa aacttcaacc
      901 aaaataggag caaggctgcc ggcctataca actgcagatg gtaaaataat tatatgttat
      961 caaaataaag ataaaattaa aaattattta ccgaaaagaa ttcctcagta tactaataat
     1021 actataacga ataaaggtga gttcattaag aaattagaag aaatgaagaa aaacggttac
     1081 gcaattgaca atgaagaata tgttgaagga ttaaaatgtg tagctgcacc gatatttgat
     1141 catgatggga aagtaagatt cagtttaagc acaacaggtc ctgctttcag gatgaatgag
     1201 gaaagaataa aggaattaat tgttattatt aaagaagctg cgaatgaaat atcacaaaga
     1261 attggatata tgaaataaat ttaggattgt cggggaacca gatcctttta aaagcatcat
     1321 acgccagaat gcagtttggc aataatgaac ataagttgaa aaataagtgt taactaatat
     1381 aatacaattg agataatttt gaggattgtt gggaaaccaa tccttttaaa gaagcttatg
     1441 tcagaacgca gtttgttaat gatgaacatt agttaaaaaa ttttcaaaaa agtgttgact
     1501 aatgtaatac aatttgttaa tattgtaccg atttaatcac aaagttataa gcagctaaat
     1561 gctgattata tatatagatt aagaatttat ggaggaaagt aatgaaaaag ttattagcaa
     1621 tagtgttgat cttagcgatg tctctatttg ctttcgtagc atgtacgcag gatcaaccgg
     1681 gtgaaacacc aaaaaatgaa gaaccaaaaa ctgaagaacc aaagactgaa gagcctaaag
     1741 aagaagaacc attagctgaa ggagcagtta aaactggttt ggcagttata tcatctgttg
     1801 agaaatcaaa ggatgcaggc gaaaaagacg gattagctca aacagattca gtaattgttg
     1861 cagttacagt tgacaaagac ggaaaaatag tgcaaactat aatcgatact gcacaaacta
     1921 aaattgaatt cagcaaagaa ggtaaaatta tacctgattt aaatactgtg tttaagtcta
     1981 aacaagagct taaagaagaa tacggaatgg gtaaagcttc aagcatagaa aaagaatggt
     2041 atgaacaagc taatgctttg gctgaatatg taaaaggcaa gactgttgaa gaagttaaag
     2101 ctattgcagt tgatgaaact ggagttacaa cagaagctga cttagtatca tccgtaacaa
     2161 tcaaaattaa tccttataaa gaagctattg aaaaagctgt agcaaatgct gagaatttag
     2221 gtgctgatgc ttctgataaa ttaggattgg gagttataac tacaattgca aaatcaaaag
     2281 atgcaacagc tgatgcagaa ggattagctc aagcttactc aacttatact gtagcaacat
     2341 ttgatgcaga cggaaaagta acaagctcaa taatagatgc atctcaagct aacgtaaaat
     2401 ttgataatac aggtaaaata actacagacc tagcagttgc acctttaact aaggttgagt
     2461 taggcgaaga ttacggaatg aaaaaagctt cagcaatagg caaagaatgg tatgaacaag
     2521 cagatgcatt tgcagattat gtggtaggca agactgttga agaaattaaa ggtatagcag
     2581 ttgatgaaag cggccatgct accgaagaag atttagtatc atctgttaca gttggaattg
     2641 tagacttaaa aaccattttt gagaaggctt ataacaatag tatttaacaa agtataacaa
     2701 tcaaagcgaa tcattattgg aggggcagat gctgcccctt gtttttatta gaggtgtaag
     2761 ttggcaaaga aaatcatttc cgtaatattg ttaatattaa taataataag tttaaccgga
     2821 tgcagtgata aagaacctat tcgctatcaa tccgaattta taatgctgtt tgatactgtt
     2881 acaagaattg tgggatattc aaagagcaaa gaagagttca ctgagtacgc acagataatt
     2941 tatgacaatt taaaggaata tcatcagctt tatgatatat ataatgacta tgaaggcata
     3001 aacaatatta agactatcaa cgataatgca ggaataaagc ctgtcaaagt agacaaaagg
     3061 ataattgatt taataaaatt ttcaaaggat atgtatgaga aaacaaacgg taaaacaaat
     3121 atagcttttg gtgccgtatt aagaatttgg catgaatatc gaaccatcgg tattgaagac
     3181 cctgcgaatg cagctcttcc accggaagaa gaattaagaa ttgcatctga gcacactaac
     3241 attgaagata taattattga tgaggagcaa tcaacagttt ttttaagaga tccttttatg
     3301 agtcttgacg ttggtgccgt agcgaaggga tatgcaaccg agcaggtaag taaaatagca
     3361 aaggaaagtg gaatgaattc cgcattattg tccgtaggag gaaacgtacg agccataaat
     3421 aaaaatgtta cctcgaatga accatggaat gtaggaatcc aaaatcccga taccgaagct
     3481 gaagaatcag ttattaaaat agttaagtta gacgacagct ctttggtaac aagcggtgat
     3541 tatgaaagat tttatatagt tgacggggag aagtacaatc atataattaa tcctgacaca
     3601 ttatatcctg caaaatattt tactgccatt tccataatat gcaaggattc gggattggct
     3661 gatgcattat caacagctgt tttctgcatg ccttttgaag agggtcttga atttataaat
     3721 tcattgcctg aagcggaagc aatgtggatt ttcagcgata aaacaataaa atacagcgat
     3781 aattttgaaa gttttattaa aaaataaagc atgagtttaa attgacacta aatgaataat
     3841 aatatagaat taataagcaa tcctgatgga ttggttattt atttttttat ttgaggataa
     3901 tatgaaaaag gaatcagagc ttaagattat accaatatta tcaaaaggat ttaaaaggtt
     3961 tttctttata tctgctgcag caactatact aaaggtttct gcaagttttg cagtgcctca
     4021 agttattagg tttaccgtag accatatcat agacgattat cccttgaact acattcaatt
     4081 ggttatgaat acaataaata atataggcgg gatagaattt ttaaaagaaa atttatggct
     4141 ccttactttt tttatattag tcttggcagt tataacggga gtaagcgatt atatcggaaa
     4201 aattaccctt gctaagggaa cggaaggaat tataaaaaat ataagggata agttgtttga
     4261 gcacatacaa aaattgccat acagctggca tattaaaaat caaacaggag atataataca
     4321 aaggtcaaca tcagatgtag aagtagttcg taattttatc agtactcaat tggtcgaaat
     4381 attcagggta atcatgctgg ttataatatc gttgatttta atgttttcaa tgaattttca
     4441 ccttactgcg gcagtattgg tatttatacc tgtagttgtg ctatattcaa cgatttttta
     4501 tggtatattg accaaaaaat tcttggttgc ggatgaagcc gaagggaagc tttttactgc
     4561 aattcaggaa aatttaacag gtgttcgtgt tgttagggcc tttggcagag aaaaacatga
     4621 aatagagaaa tttacggaaa gaaataatac attttcagag ctatgggtta agctgggata
     4681 tcagttagga tattattggg gaatcggcga tttggtaacc ggggtactcc ttatggtcat
     4741 tgttgtatac ggtactatat ttgctataaa tgatagaatt acacttggag aatttctggc
     4801 atttgcttct tataccgtaa tgatttcatg gccgcttcgc agttttggga ggattttatc
     4861 agagcttagc aaagctaagg tttcattaaa aagaatttgt gaaatattaa aggaacctgt
     4921 tgaggatgac agtccggatt ccgtcactcc ggatatctta aaggatattg aatttaaaaa
     4981 tgttacattt aaatatgatg gagaaactga ggttttgaaa aatgtcagct ttacaataaa
     5041 ggcaggaagc acatttgcta tattaggagg taccggctcc ggaaaaacta ctcttatgta
     5101 tcttttaaac cgcttgtatg atttgcctga ccatatgggc tgcattacaa taggcggagt
     5161 gagtataaaa aatattaaaa gggaacattt aagaaaaaat attggaatgg tacttcagga
     5221 gcccttttta ttttctcgaa cgattaagga aaatatagca atagtcgagc ctgatacttc
     5281 cttggatgaa attaagaaag ctgcatcaat agcgcagatt gatgaatcta tagaagaatt
     5341 ttcaaaaggc tatgatacaa ttgttggaga aagaggagta acattatcgg gaggtcaaaa
     5401 acaacgggtg gcaatagcca ggatgctgct tcaaaaaaca ccgattatgg tgtttgatga
     5461 ttcattgtct gcagtcgatg ccgacacaga tgcaaaaatt cgttcatcac ttataaaaag
     5521 caagggcagt tcgacaatta taattatttc acatcgcata acaactttaa tggaagctga
     5581 tactatttta gtcttggaca agggagaagt atccgatata ggaacccatg aagaattaat
     5641 aaacagagat ggtatttata aagacatatt caatgttcag atgaacctat cggaggatat
     5701 agaatgaatt attatgagga agagagttat agctcattta atataaagcc ttggttaaaa
     5761 ttaggaagat tttttaaacc ctacacaggg aaactgttgg gtattatctt ttttatgttt
     5821 attaccgccc taatggattt atccattcct ttgtttcaaa aatatgccgt ggacagtttt
     5881 atagcaaaga atactactga aggtataaat ggttttattg ctctttatac agcagtctta
     5941 gtgattatgg ccatatccgt aaatatattt atacgattgg caatagtagt tgaaatgcac
     6001 attgggatgg atctaagaaa ggaaatattc gaacaccttc aaaatctttc gttgtcatat
     6061 tacaacacaa ctcccgtggg ttatataatg gcaagaactt taagcgatac caataaaata
     6121 ggaagtatga ttgcatgggg attggtagat gtattttggt catttgctta tgtaacaggt
     6181 gcctttattg caatgctttt gctgaatgtg caacttactg ttattattgc gattgtaatt
     6241 cctgtaatgg cggttataac tgtttatttt caaaataaga ttttattaac cagcagaaaa
     6301 ttaagaaaaa tcaattctaa aatgacagga agtttcaatg aagggataat aggtgcaaca
     6361 acctccaaga cattggtaat agaggacaaa aacttggtgc aattcaaaaa tataagcagt
     6421 gaaatgaaga tgacggctgt taatttagca cgattaaatg ccttgtacat tcccataata
     6481 gtatttttcg gctccgtttt gacagcattg gtcttggcaa aaggaggcag attggcaatg
     6541 gataaaatta ttcaaatagg aacactgtct gcttttcttt cttatgcaat aagcatattt
     6601 gaacctgttc agcagttggc aagaacattt gcagaagtca tatcactgca ggctaatatt
     6661 gaaagagtaa cggatttatt ggaaaaggaa cccttaataa aagacagtga agaagtagtg
     6721 aaaatttacg gagacactat taatccaaaa aaggaaaact gggaagaaat cataggggaa
     6781 attgaattta aaaatgtcag ttttaaatat ccggatggag atgaagaggt tttgcaaaat
     6841 tttaatcttc atattcccgc aggtaccaat gttgcaattg taggtgaaac gggtgccggt
     6901 aaaagcactc tggtaaatct tgcatgcaga tttttcgagc ctactgaagg tgaaatactg
     6961 atagatggca aggattacag agaaagatct cagctttggc ttcattcgaa cattggatat
     7021 gtgctgcaag acccgcattt gttttccgga acaatcaagg aaaacataag atacggtaaa
     7081 ttagatgcaa cagatgaaga aataatggaa gccgcaaaaa ttgtttcagt cgataccata
     7141 atcaacaaat tggaaaaggg ttatgacagc catgtcggcg aagggggaga caggctttct
     7201 acaggtgaga agcagttgat ttccatagca agggcaatac tttcaaaccc taaaattttt
     7261 attttggatg aagcaacatc ttcggtagat acacaaacag aaatacttat ccaaaaagga
     7321 attgaatatt tgttaaaagg cagaacttca tttataattg ctcacagact ttcaactatt
     7381 aaaaaggcag atattatatt agtggttaat gagggcaaaa taatagagca agggacacac
     7441 aatgaactga tagctaaaag gggccattat tataatttat atactaaaca gtttgaagag
     7501 gaaaaaaccc ttgaagttct aaaatgaggc aaat
//
LOCUS       DAOSHP010000083         7525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8463_length_7525_cov_9.158099, whole genome shotgun sequence.
ACCESSION   DAOSHP010000083 DAOSHP010000000
VERSION     DAOSHP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8463_length_7525_cov_9.158099"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1605
                     /gene="cls"
                     /locus_tag="PLB33_07730"
     CDS             73..1605
                     /gene="cls"
                     /locus_tag="PLB33_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOG63451.1"
                     /translation="MEKIISFFVSRATLVTLALLAQIITLALMIWRFSNYFVIFDIIF
                     MVISVLVVLYILNRKSDPTYKIAWIIPIMLFPVFGGLFYLMFGGTGLSSKMKDKMHTI
                     VDKMKESLYQHPVTLNNLRKENTIAFNQAKYIEEYSSCPVYDNSPTKFLASGEEFFKH
                     LTEELKKAQKYIFIESFIIEEGIMWNTILEILKEKAKQGLDIRVIYDDFGCVTRLPHN
                     YNKILEGYGIKCSVFNPYIPILSFRLNNRNHRKIAVIDGKTAYTGGVNLADEYINAVE
                     KFGHWRDSMIEIKGDAVWSLTAMFLSMWGYLRKIDENYRDFRPDDYDMSEECHGYVQP
                     FDDSPLDSEHVGETVYLNLINQAEKYIYIETPYLIIDNKMMVALTMAAKRGVDVRILT
                     PFIPDKWYAFAVTQSNYEDLMDVGVSIYQYTPGFNHGKTFVVDDEYAVVGTINLDFRS
                     LYLHFECGVWMYNTESVGQVKESILEALKISRQVRMEDIKKTARFKKIARTVLRIFAP
                     LM"
     gene            1622..2191
                     /locus_tag="PLB33_07735"
     CDS             1622..2191
                     /locus_tag="PLB33_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG63452.1"
                     /translation="MKKNIKFYWKLFLSTFTLSAFTFGGGYVIVPLMRKKFVEELNWI
                     EEEEMLNIVAISQSAPGMIAVNTSIMTGYRLAGVFGAIIATFGTVLPPLIIITIIAGF
                     YTAFKENRIINALLLGMRAGVAAVIIDVIIKMARNVIKTKNNISIAIMVTALIAAVVF
                     DINAALIIILSGVFGGFYYNIVKRTGESQ"
     gene            2188..2760
                     /locus_tag="PLB33_07740"
     CDS             2188..2760
                     /locus_tag="PLB33_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOG63453.1"
                     /translation="MIYLEIFLSFFQIGLFSFGGGYAALPLIEAQVLEYHNWLTIEEF
                     ADLLTISQMTPGPIAINAATFVGTKVAGLPGAVIATIGSVTPSFIIVLTLSYFYFRYK
                     NLSSIQNILKGLRPAVVSLIASAGLSILMLTVFKTEGITLLNIKLSNINFVAIIMVVI
                     SVFILRKFKIDPIKVMLGTGVLGVLVYGLF"
     gene            2775..6251
                     /locus_tag="PLB33_07745"
     CDS             2775..6251
                     /locus_tag="PLB33_07745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOG63454.1"
                     /translation="MSNNKFTHLHLHTEYSLLDGACRIKELVKRAKELNMDSLAITDH
                     GSMYGVVEFYKQARKEGIKPILGFEAYVSPRKMTDKDPQNDKTQYHLVLLAENREGWQ
                     NIIKLCSIGFVDGYYYKPRIDHETLKEYSSGIIALSACLAGEVQTYLLEDNYDEALKT
                     ALLYKDIFGENNFFLELQDHGMEEQKAVNSSLLKLSKETGIKLVATNDVHYINKDDAY
                     FHDVLLCIQIQKTIVDEDRMKFPSDEFYLKSYEEMAELFPEEVLRNTADIAERCNVEL
                     DFNTVHLPEFKVPKGYEKSEYFKKICQEGLKERYKNITEEIQQRLDYEIGVIEQMGYV
                     DYFLIVWDFIKYARDNNIMVGPGRGSAAGSLVAYSMKITNIDPLKYNLIFERFLNPER
                     ISMPDIDVDFCYERREEVIDYVKEKYGQERVAQIITFGTMAARGSIRDVGRALNMPYG
                     EVDFIAKKIPMTLGMTISTALEVNKELKDLYDSDIKVNKLINLAMKVEGLPRHSSTHA
                     AGVLISKEEVTKYVPLSRNKDVVTTQFNMVELEELGLLKMDFLGLRTLTVIRDAIELI
                     KENHNIKIDFQNCNYDDKEVYKLFANAETLGIFQFESSGMRAFLSELKPSEFENLSAA
                     NALYRPGPMGQIPVYIKNKLNPDKVTYLHPKLEPILNVTYGCMVYQEQVMQIARDIGG
                     FTMGRSDSLRRAMGKKKMDVMLKERQNFIYGKRAENGEPEIKGAVKNGVDEKTANEIY
                     DLMVEFAKYAFPKAHSVAYAALAYETAWLKTYYPVEFMAALISSIMGSAGTVSLYIRE
                     CKRLGIEILPPDINESMGKFTVENGKIRFGLAAVKNVGVNVIQEIVKAREKKGKFTSF
                     ADFCSKVDPLVLNKRQIESLIKCGAFDFLNVRRSQLMAIYERTIDSVLAQKKRNIEGQ
                     FSLFDSMETEVDIVDELPELPEYNEKALLSMEKEMVGIYLSGHPLSEYEKILEKYATT
                     NSSEISEIMDANEGNVLYDGSRVVMGGIIIKKQNKITKNNNMMAFITLEDLYGTVEGI
                     IFPKIYDDCKDILYEDNIVLVEGTIDTSEEESPKLIINKVTELKKEYENDIKRSKLYI
                     KVKDTESYKNLKKDLFYCICKHKGEDCVIIYNEKDKASMVLSDKYRVNTEDEILFKDL
                     KKLVGEKNIAII"
     gene            6270..7229
                     /gene="pfkA"
                     /locus_tag="PLB33_07750"
     CDS             6270..7229
                     /gene="pfkA"
                     /locus_tag="PLB33_07750"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOG63455.1"
                     /translation="MRKLAVLTSGGDSPGMNAAIRAVVRYSIYNNISVIGIRNGFQGL
                     VEGDHTEMTLSSVADIIHRGGTILGSARSDEFMKEEGFSKALKNLKYLGIEGLVIIGG
                     NGSFKGAQKLEKAGVSTVCIPGTIDNDLPYTRYTLGFFTALTTVLESVEKIRDTSSSH
                     NRSNVIEVMGRNCGDIALYAGLAGGAEMVIIPEIEYNIDEICDKIMQGRTRGKRHSII
                     ILSEGAGDAREISELIEKKTGIITRYSILGYTQRGGAPAAYDRLIGSQMGAYAVKLLC
                     GNLRNRAVGVNGGGIFDMDLDEAVSVEKVFDKTTYELTKILSI"
     gene            7355..>7525
                     /locus_tag="PLB33_07755"
     CDS             7355..>7525
                     /locus_tag="PLB33_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOG63456.1"
                     /translation="MRKTKIVCTIGPSSQGEETFKELVINGLNVARLNFSHGTHEEHK
                     EKINTIKKVREEL"
BASE COUNT         2684 a         1164 c         1620 g         2057 t
ORIGIN      
        1 ctttaaaact catttacaaa tacttcattt tttgatacaa tttacgtaat atatatagaa
       61 aaagggagct ttatggaaaa aattattagc ttttttgtaa gcagagccac attggtaaca
      121 ctggcactct tggctcaaat aattacactg gcattgatga tatggagatt cagcaattat
      181 ttcgtaattt tcgatataat cttcatggtc atcagcgttc tggtggtatt gtacatatta
      241 aatcgaaaaa gcgaccccac ttataaaata gcatggatta ttccaattat gctttttcca
      301 gtgtttggcg gattgtttta tttgatgttc ggagggacag gattatcaag taaaatgaaa
      361 gacaaaatgc atacgattgt agataaaatg aaagaatccc tttatcaaca tcccgttact
      421 ttgaataatc ttagaaaaga aaatactatt gcgttcaatc aagccaagta catagaggaa
      481 tactcgtcat gtcctgttta cgataattct ccaacaaaat ttttggcatc aggtgaagag
      541 tttttcaagc atttgactga agaactaaaa aaagcccaga agtatatatt tatagagtct
      601 tttataattg aagaaggtat aatgtggaat acaattttgg aaattctaaa agaaaaagca
      661 aagcagggct tggacataag agttatttat gatgacttcg gatgtgtgac aagacttcct
      721 cacaactata ataaaatact agagggatat ggaattaaat gcagtgtttt caacccctac
      781 ataccgattt tgtcatttcg cctcaataac agaaaccaca ggaaaatagc ggtaatagac
      841 gggaaaaccg cttatactgg aggagtaaac ctagcggatg aatatattaa tgccgttgaa
      901 aaattcggac actggagaga cagtatgatt gaaataaaag gagatgcagt gtggagttta
      961 acggcgatgt ttttatccat gtggggctat ctaaggaaaa tcgatgaaaa ctatcgggac
     1021 tttagacctg atgattatga tatgagtgaa gaatgccacg gatacgtcca gcctttcgat
     1081 gacagcccct tagacagtga gcatgtcggc gaaaccgtat atttgaattt aatcaaccaa
     1141 gcggaaaaat acatatacat agaaacacca tacctcatta tcgataacaa aatgatggta
     1201 gcattaacaa tggcagcaaa aagaggagtg gatgtaagaa ttcttactcc cttcataccg
     1261 gacaaatggt atgcatttgc tgtaactcaa tctaattatg aggatttgat ggatgtagga
     1321 gtgtctattt accaatacac tccgggcttt aaccacggca agacatttgt tgtagatgat
     1381 gaatatgcgg tggtaggcac cataaattta gatttcagaa gcctttatct gcattttgaa
     1441 tgcggagtat ggatgtataa cacagaaagc gtcgggcagg ttaaagagag tatattggaa
     1501 gctttaaaaa taagcaggca agtaagaatg gaagatatta aaaaaacagc tagatttaaa
     1561 aaaatagcaa ggactgtact cagaatattt gccccgctga tgtagacaaa cgaggaccta
     1621 tatgaaaaaa aacattaaat tttactggaa attattttta tcaactttta cgttaagcgc
     1681 ttttactttc ggaggaggat atgtaatagt acctcttatg aggaagaaat ttgtcgaaga
     1741 acttaattgg atagaagaag aagaaatgct gaacatagtt gccatatccc agtctgcacc
     1801 aggcatgatt gctgtcaaca cttcaattat gaccggctac aggcttgcag gagtatttgg
     1861 agctattata gctacttttg gaacagtact gccgccttta atcataataa cgataatagc
     1921 cggattttat acagctttta aagaaaaccg cattataaat gcccttttat tggggatgcg
     1981 tgccggtgtt gctgccgtaa taatcgatgt aataataaag atggcaagaa atgttataaa
     2041 aaccaagaat aatatttcca tagcaattat ggttactgcc ttaattgctg ccgtagtttt
     2101 tgacataaat gccgctttaa taataatact tagcggagtt tttggaggat tctattacaa
     2161 catagttaaa aggaccggtg aaagtcaatg atatatttag aaatattctt gagttttttt
     2221 cagataggat tattcagctt cggaggaggt tatgctgcac ttcctttaat agaagctcaa
     2281 gttttggaat atcataactg gcttacaata gaagaatttg cagatttgct gaccatatcc
     2341 cagatgacgc cgggacctat tgcaattaat gcagcaacat ttgtgggaac taaggttgcg
     2401 gggctccccg gagccgtcat tgctacaata gggagtgtaa ccccttcttt cattatagta
     2461 ttaacactgt catatttcta ttttagatat aagaatttat catcaattca aaatatactg
     2521 aagggattaa gaccggcggt tgtatcatta attgcttctg cggggctatc cattctaatg
     2581 ttgacagtat tcaaaacaga aggaataacc ctgttgaata ttaaattaag caatatcaat
     2641 tttgttgcaa taataatggt ggtaataagt gtttttatac ttagaaagtt caaaattgac
     2701 ccgataaaag taatgttagg gacaggggta ttaggagtat tggtttacgg actgttttga
     2761 tataaggaga aaaaatgtca aataacaaat ttacacatct tcatttgcat acagaataca
     2821 gccttcttga cggagcctgc agaattaaag agcttgtaaa aagagccaag gaattaaata
     2881 tggattctct ggctataacc gaccatggga gcatgtacgg agtagttgag ttttataaac
     2941 aagcaagaaa agaaggaatt aaaccgatac ttggatttga agcatatgta tctcctcgta
     3001 aaatgaccga taaggaccct caaaatgata aaactcagta tcatttagtg ctcttggcag
     3061 aaaacaggga gggctggcaa aatataatca agctttgttc cataggtttt gtggatggtt
     3121 actactacaa gccaagaata gaccatgaaa cattgaaaga atacagcagc ggaattattg
     3181 cattaagtgc atgtcttgca ggtgaagtac agacatattt attggaagat aattacgatg
     3241 aagcattaaa gacagccttg ttgtacaagg acatattcgg agaaaacaac tttttccttg
     3301 aattgcagga ccacggcatg gaagagcaaa aagccgtgaa cagctccttg ttgaagctca
     3361 gcaaagaaac aggcataaag ttggttgcaa caaatgacgt tcattatata aacaaggatg
     3421 atgcttactt tcacgatgtg cttctttgta ttcaaatcca gaaaacaata gtggatgaag
     3481 ataggatgaa attcccgtcg gacgaatttt acctgaaatc ctatgaagaa atggctgaac
     3541 ttttccccga agaagtactg agaaataccg cagatattgc agagagatgc aatgtagagc
     3601 tagattttaa cactgtgcac ctgcctgaat ttaaagttcc taaggggtat gagaaatcag
     3661 aatatttcaa aaaaatatgc caagagggat tgaaggaaag atataaaaat atcactgaag
     3721 aaattcagca aagattggac tatgaaatag gtgtaattga gcagatggga tatgtggatt
     3781 attttctcat agtgtgggat tttatcaaat atgccaggga caacaacatt atggtgggac
     3841 ccggaagagg ctctgcagca ggaagcttgg ttgcatattc catgaaaata acaaatattg
     3901 accctttaaa atacaacttg atttttgaaa gatttttaaa tcccgagcgt atcagtatgc
     3961 cggatattga cgttgatttt tgctatgagc gccgtgaaga agtaatagac tatgtaaaag
     4021 agaaatacgg tcaggaaaga gttgcccaga taataacatt cggcacaatg gcagcaagag
     4081 gctccatccg tgatgttgga agagcattaa acatgcccta cggcgaagtg gattttatcg
     4141 ccaaaaaaat ccccatgaca ttgggaatga caataagcac agccttggag gtaaacaaag
     4201 aattaaagga tttatatgac agcgatataa aagtcaataa gctgataaat ctggccatga
     4261 aagttgaggg gcttccaaga cattcttcaa cgcatgcagc cggtgttctc atatccaagg
     4321 aagaagtaac aaagtacgtt cctctttcaa gaaataaaga cgttgtaaca actcaattca
     4381 atatggttga gcttgaagag ctgggactgt taaaaatgga cttcctaggg ttaagaactc
     4441 ttacggtaat acgtgatgcc atagaactaa ttaaagaaaa ccataatata aaaatagatt
     4501 ttcaaaattg caattatgat gacaaggaag tttacaagct ttttgcaaat gctgaaacgc
     4561 tgggtatttt tcagtttgaa agttcaggca tgagagcatt tttaagcgag ctgaagcctt
     4621 cggaatttga aaacctctca gccgccaatg ctctttacag gccgggcccc atggggcaga
     4681 tacccgtata tataaaaaat aagctaaatc ctgataaggt tacctatctt catccgaaat
     4741 tagaacccat tcttaacgta acctatggct gcatggtata ccaggaacaa gttatgcaaa
     4801 tagcaagaga tatcggcgga tttacaatgg gtcgctccga ttccttaagg cgtgccatgg
     4861 gtaagaaaaa aatggacgtc atgctaaagg aaaggcaaaa cttcatttac gggaaaagag
     4921 cggaaaacgg ggagcctgaa ataaaaggtg ctgttaaaaa cggtgttgat gaaaaaacag
     4981 ccaatgaaat ttatgattta atggttgaat ttgctaaata tgcatttcca aaggcgcatt
     5041 ccgtagctta tgccgccctt gcatatgaaa ctgcatggct aaaaacctat tatcccgttg
     5101 aatttatggc agcgctcata tcaagcatta tgggaagcgc cgggacagta tctctataca
     5161 ttagagagtg caaaagactt ggaatagaaa tacttccgcc ggatattaat gaaagtatgg
     5221 gaaaatttac ggtggaaaac gggaaaatac gctttggctt agctgccgta aaaaatgtag
     5281 gggtaaatgt aatacaagaa atagttaagg caagagaaaa aaagggtaag tttacaagct
     5341 ttgctgattt ttgctcgaag gttgaccccc ttgtattaaa caaaaggcaa attgaatcat
     5401 tgataaaatg cggcgctttt gactttttaa atgtaagacg ctcacagctt atggcaattt
     5461 atgagaggac aatcgattca gtgttggctc aaaagaaaag aaatatcgaa ggtcagtttt
     5521 ccctttttga ttcaatggaa acagaagtcg acattgtgga tgaactgccg gagctgcctg
     5581 aatataacga aaaagctctt ttatccatgg aaaaagaaat ggttggaata tatttaagcg
     5641 ggcatcctct ttctgaatac gaaaagatat tggaaaagta tgctacaaca aattccagtg
     5701 aaatatcaga aataatggat gctaatgaag gaaatgtttt atacgatggc agcagggttg
     5761 ttatgggcgg aataattata aaaaaacaaa ataaaataac gaaaaataac aatatgatgg
     5821 catttataac attggaagac ttgtacggaa ccgtggaagg aataattttt ccgaaaattt
     5881 acgatgactg taaggatatt ctatatgaag acaatatagt attggttgaa ggaacaatag
     5941 acacctccga agaagaatca ccaaagctta ttataaataa agtgaccgaa ctgaaaaaag
     6001 agtatgaaaa tgatattaaa cgaagtaaat tgtatataaa ggtgaaggat acagagagct
     6061 ataagaatct aaaaaaagat ttattttatt gtatatgtaa acacaaagga gaggactgtg
     6121 ttattatcta taacgaaaaa gataaagcaa gtatggtttt atcagataaa tatagagtaa
     6181 atacagaaga tgaaatatta tttaaagact taaaaaaact ggttggagaa aagaatatcg
     6241 caattatata gttgtgagag gaggtagcga tgagaaaatt agcagtatta acaagcggag
     6301 gcgactcacc gggcatgaat gcagccataa gggcagttgt cagatactct atttataata
     6361 acataagtgt aataggaatt cgcaacggat tccagggttt ggttgaggga gaccatacgg
     6421 aaatgactct atcctctgtt gcagacatta ttcacagagg agggactatc cttggaagtg
     6481 ctagaagcga tgagtttatg aaggaagaag gctttagcaa agccttaaaa aatttaaagt
     6541 acttggggat agaaggattg gtcataatag gaggaaacgg ctcttttaag ggcgcacaaa
     6601 agcttgaaaa agcaggtgtt tccactgtgt gtattcccgg gaccatcgat aatgatttac
     6661 catatacgag gtatacctta ggatttttca cagctctcac aactgtattg gaatcggttg
     6721 aaaaaataag agatacatca tcatctcaca acaggtcaaa tgttattgag gttatgggaa
     6781 gaaactgcgg cgatattgca ctttacgcag ggcttgcagg cggggctgaa atggttatta
     6841 ttcctgaaat tgaatataat atagatgaaa tatgcgataa aataatgcag ggaagaacca
     6901 gagggaaaag acacagcatt ataattcttt cggaaggagc aggtgacgcg agggaaatat
     6961 cagagctgat agagaaaaag acagggataa taacaagata ttccatactt ggttatacgc
     7021 aaagaggcgg agcacctgca gcttacgaca gattaatcgg aagccagatg ggagcttatg
     7081 ccgtgaaatt attatgcggt aatttaagaa acagagccgt gggagttaac ggaggcggaa
     7141 tttttgatat ggatttggat gaagccgttt ctgttgaaaa agtatttgat aaaacaacat
     7201 atgaattaac aaagatactg tcaatctaat gtatagggaa aacggagaca ctggggacga
     7261 tcctttttgt cccacttaat gacactaccc ccgtcccctt gtcattttag ggacaacgaa
     7321 gcaccatacg agggagttga aatatatgct gaatatgcgc aaaacaaaaa tagtatgtac
     7381 catcggtcct tcatctcaag gtgaagaaac gttcaaagaa ttggttataa acggtttaaa
     7441 tgtggcaaga ttgaactttt cccacggtac acatgaagaa cataaagaaa aaataaatac
     7501 tataaaaaag gtgcgagaag aacta
//
LOCUS       DAOSHP010000084         7386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_8693_length_7386_cov_9.100123, whole genome shotgun sequence.
ACCESSION   DAOSHP010000084 DAOSHP010000000
VERSION     DAOSHP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_7386_cov_9.100123"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1426)
                     /locus_tag="PLB33_07760"
     CDS             complement(158..1426)
                     /locus_tag="PLB33_07760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOG63457.1"
                     /translation="MAKEVLNPLENSQMQVKKACDALGLHPDVYEILKEPQRVIEVNI
                     PVRMDDGSMKVFKGFRASHNDAVGPAKGGVRFHPGVTLEEVKALSIWMTFKGGVMGLP
                     YGGGKGGVICDPLTLSQGELERISRGYIQKLYKYIGEKEDIPAPDAGTNGQIMAWMVD
                     EYIKLTGGSASLGVITGKPVAWGGSEGRNEATGFGIAVVTREAAKKYGIDLTKAKVAV
                     QGFGNVGRYTVKNIQRQGGTVVAVGEWAPKVGTYAIYNEDGLDFEDLLAYFNEHKNLV
                     DYPKAKMISLDEFFKLEVDILIPAALENAITKEVAENIKAKLVCEAANGPTTPEADQV
                     LNERGIPVTPDILTNAGGVVVSYFEWVQNLQGYYWDEKEVEEKQEIKMVKAFNDVWAL
                     KEQQNCTIREATYMISVKKVAEVMKLRGWY"
     gene            complement(1649..2296)
                     /locus_tag="PLB33_07765"
     CDS             complement(1649..2296)
                     /locus_tag="PLB33_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOG63458.1"
                     /translation="MDKHQKISSAVIRRLPKYYRYLGELNKVGITKTSSRELSEMTGF
                     SASQIRQDLNNFGGFGQQGFGYDVGNLRNEIGKILGLDKKYKIIIVGAGNIGQAIANY
                     TGFYEADYEVVALFDKNPKLVGLSIRNALIMDSDNLEEFLKKEHIDIAVICTPKSVSQ
                     QVAEQLVRCGIRAIWNFAPKDLKMPEEVYVENVHLNESLFSLTYYYNKMKKESGK"
     gene            complement(2399..3016)
                     /locus_tag="PLB33_07770"
     CDS             complement(2399..3016)
                     /locus_tag="PLB33_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOG63459.1"
                     /translation="MARKTDYFEGFIKLAEYSYNAAKLLDDTLRDFNKDSLQKTMKLM
                     HEIEHTADLEGHEITKKLLKEFITPIEREDIMLLIHKIDDITDCIEDVLLHMYMYNVR
                     VIKEDAIKFSGLILSSCEELIGALKEFKNFKKSKEIRSRIIAVNKIEEEGDNLYTEIV
                     RKLHLTSMNAIDIMTWTIAYNRLEKCCDACEEAANVIESIILKNL"
     gene            complement(3028..4080)
                     /locus_tag="PLB33_07775"
     CDS             complement(3028..4080)
                     /locus_tag="PLB33_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOG63460.1"
                     /translation="MAISFSDYLMQITDNPMLLITSILTMAVILVNGWTDAPNAIATC
                     VSTRSISAKSAIVMAAIFNFLGVLLMTVINANVAQTIYNMVDFGGNSYDALVALCAAL
                     FAIVIWATAAWWFGIPTSESHALIAGISGAAIALHNGLQGINGSEWIKVLYGLGLSTF
                     LGFTLGWIVVRMVEIIFRSMSRMKANILFTNAQVASGAAMAFMHGAQDGQKFMGVFML
                     GIYLAGGQANVTTFQIPLWMMILCSAIMGIGTSIGGYRIIKSVGMDMCRLQKYQGFSA
                     DMAAATCLFISSMTGIPVSTTHTKTTAIMGVGAARRFSSVNWGIVGEMVMTWILTFPG
                     CGIIGYLMAKLFINIF"
     gene            4244..5251
                     /locus_tag="PLB33_07780"
     CDS             4244..5251
                     /locus_tag="PLB33_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOG63461.1"
                     /translation="MKKFISLLVVMLLITSLVLTGCNEKPTEYQKVRLIEVTHSIFYT
                     PQYVALEMGLFEKQGLSIELTNGGGADKCMAALVSGEADIAFMGPEASVYVYLQGRDD
                     LAVNFAQLTQRDGSFIVGREKDDNFTIDKLKGHTILGGRKGGMPLMALEYVLKQNGLT
                     PGVDVNVRTDVQFDMMAGAFASGEADYTTLFEPLATSFELQGNGYVVESVGRLAGYIP
                     YTCYSALESYIEKNPEIIQGFSNALYEAMVWVDEHTAMEVAEVIQPQFADTDIDFLAK
                     IVQTYRDLDAWNPDLVLGEDGYNRLIDIMKLAGEIEEGAPYSVIQTPEFAIKAKENYK
                     K"
     gene            complement(5343..6407)
                     /locus_tag="PLB33_07785"
     CDS             complement(5343..6407)
                     /locus_tag="PLB33_07785"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOG63462.1"
                     /translation="MAHNKIISLILIIFIAVCLTACGTLDMMVGLKDENNPDIADLSD
                     LTDFEIVEDTIGEEALEEPYEEVIETVTISTEEAKMTNIMAYYEDEYGFVVPVNTKIP
                     WEEGIAKATLRSMVRGSETEKRIAQSGLRGVLPEGTEIRGMSIKDGVCRVDFSKNILN
                     TSTLKQEENLISAITYALTEFPTIDKVELLVDGQALSSLTNGYAINKGFERENINLYG
                     SADGVNYTVYYKAPDTEVMGHYVPITFSAAKVGNPVEFVMEKLFEGPPADTTLSNNIP
                     VGINLRDVKVIGDSAVVNLGVEAVNLSEEDYKDMNSIVVLCLEQYGISDVEFNIEGLS
                     FEQAGLYFEDSNVTPVFNQY"
     gene            complement(6478..7278)
                     /gene="pgeF"
                     /locus_tag="PLB33_07790"
     CDS             complement(6478..7278)
                     /gene="pgeF"
                     /locus_tag="PLB33_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOG63463.1"
                     /translation="MYKLNKIKDFEYLTFPIFDGYDELFHCFTTRKGGVSKGDFESMN
                     LGFNTGDDEEDVRENYYILSECLNINLEDIVKTFQSHTDNIRYVTEEDKGRVMKKAKY
                     TDIDGLYTDRKNIALMSFHADCNAVFFYDPVKKIIGLSHAGWKGTMTNITGKMIKALK
                     DLGSNPSNLKAAISPSLGQCCFEVDKDVADMFINTDIKYKDFMIKKGEKYHFDLWGIN
                     KYNIIREGMKEENIEVAGMCTKCRNDLFFSHRGQKGKRGLMSGIIMMK"
BASE COUNT         2236 a         1520 c         1080 g         2550 t
ORIGIN      
        1 aactgttaaa tatttaaatg atgaagggaa aataaaattg tttaaatgat gagatacttc
       61 acttcgttca aaatgacgct ccgccatatt catagcaatg acaaataaaa agcaaggctg
      121 aaccttgctt tttatatttg acgacgtgta tcttatatta ataccatcct cttaatttca
      181 taacttcagc taccttctta actgaaatca tataagtagc ttctctgatt gtacagtttt
      241 gttgttcctt aagagcccat acatcattaa atgcttttac cattttaatt tcttgttttt
      301 cttctacttc tttttcatcc caataatatc cttgaaggtt ctgtacccat tcaaagtatg
      361 aaacaaccac tccgccggca tttgttaata tatcaggagt aaccgggatt cctctttcat
      421 tcaatacttg gtctgcttca ggagttgtcg gaccgttagc tgcttcacat actaatttag
      481 ccttaatatt ttctgctact tctttagtta tagcattttc caaagctgca gggattaata
      541 tgtcaacttc taatttaaag aattcatcaa gactaatcat tttagctttt ggataatcaa
      601 ctaaattctt atgttcatta aagtaagcta ataagtcttc aaaatccaat ccatcttcat
      661 tgtatatagc atatgttcca acttttggtg cccattctcc tacagctact actgtaccgc
      721 cttgtctctg aatattttta actgtgtaac ggcctacgtt accaaatccc tgtacggcaa
      781 cttttgcttt tgtaagatca attccatatt tttttgctgc ttctctggta actactgcaa
      841 taccaaaacc tgttgcttca tttctgcctt ctgagccgcc ccatgctacg ggttttccgg
      901 ttataacacc taatgatgca gagccgccgg ttaatttgat atattcatcc accatccaag
      961 ccataatttg tccgtttgtt cctgcgtcag gtgccggtat atcttctttt tctcctatgt
     1021 acttatacag tttttggata tagcctctag aaattctttc taattctcct tgagataatg
     1081 tcaatggatc acatataact ccacctttac ccccgccata aggaagaccc attactccgc
     1141 ctttaaacgt catccagata gaaagagctt taacttcttc caatgttacg ccgggatgga
     1201 atctaactcc gccttttgca ggaccaacag catcattatg cgatgctctg aagcctttga
     1261 atactttcat gcttccatca tccattctaa caggaatgtt gacttcaatt actctttgtg
     1321 gttcctttaa aatctcatat acatccgggt gcaatcctaa agcgtcacaa gcttttttta
     1381 cttgcatttg agagttttcc aatggattta aaacctcttt tgccattact atacctccaa
     1441 atttattagt ttataagtat ttttatcttt aatattgcta aattaataac aatattatcg
     1501 gacaatctta ttctatcacc catttcaaat tatttcaagg ataaaaaata ttatttgcac
     1561 taacaagaaa atttattaat ataaattttt ttcatacaaa aaaccattga atgcaaaagc
     1621 agaagcctag acttctgctt attatgcatt attttccgct ttcttttttc atcttgttgt
     1681 aataataagt tagagaaaat aaactttcat ttaaatgaac gttttcaaca tatacttctt
     1741 caggcatttt taaatccttt ggagcaaagt tccaaatagc ccttatgccg caacgcacca
     1801 attgctcggc aacttgttgg cttacactct ttggagtgca gataactgca atgtctatat
     1861 gctctttttt aagaaattct tctaaattat ctgaatccat tatcaatgca tttctgatag
     1921 ataatcctac aagcttcgga tttttatcaa acaacgcaac aacttcatag tcagcttcat
     1981 aaaaacctgt ataattagca attgcctggc ctatgtttcc tgcacctaca attataattt
     2041 tatacttttt gtctaatcct agtatttttc caatttcatt acgcagattt cccacatcgt
     2101 agccaaatcc ctgctgtccg aatcccccaa agttatttaa atcttgtctt atttgtgaag
     2161 cagagaaacc tgtcatttca cttagttctc ttgaagaagt tttggtaatt ccaactttgt
     2221 ttaattcccc taagtaccta taatatttag gtaaccttct tattactgca gatgaaattt
     2281 tttgatgttt atccatatat gctgcctcct atggatatat tttatcaatc ttgcatttat
     2341 attgcaacac attttttaac aaatatagag aattttttaa caatctttat cttttcgctt
     2401 ataaattctt caaaataata ctttctatga cattagcagc ttcttcacaa gcatcacaac
     2461 atttttcaag tcgattgtac gctattgtcc atgtcataat atctatggca ttcattgatg
     2521 taagatgcag ctttcttaca atttctgtat aaagattatc tccttcttcc tcaatcttat
     2581 tcacagcaat aattctgctt cgtatttcct tcgatttttt gaaattttta aattctttta
     2641 atgctccgat taattcttca caactgctta aaatcaatcc ggaaaatttt attgcatcct
     2701 ctttgataac ccttacgtta tacatataca tatgaagcag cacatcttca atacaatctg
     2761 taatatcgtc aattttgtgt atcaaaagca taatatcttc acgttcaata ggggtaataa
     2821 attcctttaa aagcttttta gttatttcat gaccttccaa atcggctgta tgctctattt
     2881 catgcattaa tttcatcgtt ttctgtaaag aatctttatt aaagtccctc aatgtatcgt
     2941 ctaagagctt tgcagcatta tagctgtatt cggctaattt tataaagcct tcaaagtaat
     3001 ctgttttcct tgccatattt cacctcatta aaatatattt ataaataatt tcgccattaa
     3061 atatccgatt attccacatc ccgggaatgt caaaatccat gtcataacca tttcaccgac
     3121 aattccccaa tttactgaag aaaatcttct tgctgctccc acacccatta ttgcagtagt
     3181 cttagtatga gtagtgctta ccggtattcc ggtcattgaa gaaataaaca aacaagtggc
     3241 agcagccata tccgcagaaa aaccttgata tttttgcaac ctgcacatat ccattccaac
     3301 tgatttgatt atcctataac cacctattga tgttccgatg cccataatag ctgagcataa
     3361 tatcatcatc cataaaggga tttgaaaagt ggttacattt gcttgtccac ctgctaaata
     3421 tataccaagc ataaatactc ccataaactt ttgtccgtct tgagcaccgt gcataaatgc
     3481 catagctgct ccgcttgcaa cttgagcatt tgtaaaaagg atattggctt tcatcctgct
     3541 cattgaccta aaaataatct ctaccatcct tacaacaatc caacctaggg taaagcctaa
     3601 aaaggtagat aaacctaatc catacaatac cttaatccat tcactgccgt ttattccttg
     3661 taacccgtta tgcaaggcaa tggcagcacc tgatataccc gctatcagtg catggctttc
     3721 acttgttgga attccaaacc accatgcagc ggtagcccat attacgattg caaataatgc
     3781 tgcacataaa gcaaccagtg catcatatga attacctcca aagtcaacca tgttataaat
     3841 tgtttgtgcc acatttgcat taattactgt catcaataaa acacccaaaa aattaaaaat
     3901 tgcagccatt acaatcgctg atttcgctga tatggaacgg gtagaaacgc atgtggcaat
     3961 agcgtttggt gcatcagtcc aaccattgac caagataacc gccatggtta aaatcgaagt
     4021 aataagcaac atggggttat cagtaatctg cattaaataa tccgaaaaag atatagccat
     4081 attattctcc gtctgctact catgttttta ttatatatat attgcataca aagataatat
     4141 accatagaat ccattacttt tccactactt ttataaagag ttatgtaacg ctttagagat
     4201 aaattgaata ttatattcga aagactaatt ttgggaggat gaaatgaaaa agtttatttc
     4261 tctgcttgtt gttatgctgc tcattacatc attagtactt acaggatgca atgaaaaacc
     4321 cacggaatat caaaaagtac ggcttataga agtaactcac tcgatttttt atacacctca
     4381 gtatgttgca cttgaaatgg gattatttga aaagcaaggt ctaagtattg aattgactaa
     4441 cggcggaggt gccgacaaat gtatggcggc cttggtaagc ggcgaagccg atatagcttt
     4501 tatgggacct gaagcaagcg tctatgtcta cctacaggga agagatgact tggcagttaa
     4561 ctttgcacag cttactcaac gtgatggctc atttattgtc ggaagggaaa aagacgataa
     4621 ctttaccatt gacaaattaa aaggtcatac gattcttggc ggcagaaaag gcggaatgcc
     4681 cttaatggca ttggaatatg tattaaagca aaatggattg actcccggcg tggatgtaaa
     4741 tgtaagaaca gatgttcaat ttgatatgat ggctggagca tttgcttccg gagaagcaga
     4801 ttacactact ttgtttgagc cgttggctac atcatttgaa cttcaaggga acggatatgt
     4861 tgttgaatcc gttggaagat tagcaggata tatcccttat acatgctata gcgcattgga
     4921 aagttatatt gaaaagaatc ctgaaattat tcaaggattt tcaaatgcct tgtatgaagc
     4981 aatggtatgg gtagacgaac atactgcaat ggaagtagct gaagtaattc agcctcaatt
     5041 tgctgatacc gatattgatt ttttagcaaa aatagttcag acatataggg atttagatgc
     5101 ctggaaccct gaccttgtgc ttggagagga tggttataac agacttattg acataatgaa
     5161 gttagcaggt gaaattgaag aaggtgctcc atattcagta atacagacac ctgaattcgc
     5221 aataaaagca aaagaaaact acaaaaaata acatacaaaa tccgaggaga ataactcctc
     5281 ggatttttac ttactccacg cagtcattat atgaactgtc atcctgattg tcagcgtttg
     5341 tgctaatatt ggttaaacac gggtgttacg ttactgtcct caaaatataa tcctgcctgc
     5401 tcgaaagaca atccttcaat attgaactca acatcgctta tgccatactg ctctaaacaa
     5461 agcactacta ttgaattcat atccttgtag tcttcttccg acaaattaac agcttcaaca
     5521 cctagattaa ctactgcact atccccgata acttttacat cccttaaatt aatacccaca
     5581 ggtatattgt tgctgagagt tgtatctgca ggaggtcctt caaacaattt ttccataaca
     5641 aattctacag gattgcccac cttagctgct gaaaatgtta ttggtacata atgccccatt
     5701 acttcagtat cgggagcctt gtaataaacg gtataattta caccatctgc cgagccgtat
     5761 aaattaatat tttctctttc aaatccctta ttgattgcat acccgttggt aagggaagac
     5821 aatgcctgcc cgtctaccaa taattcaacc ttatcaatgg ttgggaattc agtaagcgca
     5881 tatgtaattg cagaaatcaa attttcttcc tgttttaagg tagaagtatt taaaatattt
     5941 ttgctgaagt ccactctgca tacgccgtcc ttgatagaca tgccccttat ttcggtacct
     6001 tcgggaagta cgccgcgtag tcctgattgt gcaattcttt tttcagtttc acttcctcta
     6061 accatacttc taagtgttgc ttttgcaatt ccttcttccc acggaatttt tgtgtttaca
     6121 ggaaccacaa agccgtattc atcttcataa tatgccatta tgtttgtcat tttagcttct
     6181 tcggtagaaa ttgtaacagt ttctatgact tcttcatatg gttcttctaa tgcttcttct
     6241 ccaattgtgt cttcaacgat ttcaaaatct gtcaaatcag acaaatcggc tatatccgga
     6301 ttattttcgt ccttcagacc caccatcata tctaaggtgc cgcatgcagt taagcatact
     6361 gcaataaaaa taatcagtat cagagatatt attttattat gagccaattt cataccctcc
     6421 tcacgcctta tatatattat tatatttagc atgtaccgaa agtatgactt taaaaaatta
     6481 tttcatcatt attattcctg acatgagacc cctttttcct ttttgacctc tgtgggaaaa
     6541 gaacaaatca ttgcggcatt tggtacacat acctgcaacc tctatgtttt cttctttcat
     6601 tccttctctt attatattat atttattgat tccccacaaa tcaaaatgat acttttctcc
     6661 cttttttatc ataaaatctt tgtatttaat atctgtgttt ataaacatat ctgcaacatc
     6721 cttatcaact tcaaaacaac actgccccaa agaagggctt atggctgcct ttaagtttga
     6781 aggattgctc ccaagatctt ttaatgcctt aatcattttt cccgtaatat ttgtcattgt
     6841 tcctttccat cctgcatgac ttagtccgat tattttttta accgggtcgt aaaagaatac
     6901 tgcattgcaa tctgcatgaa aagacatcaa tgcaatattt ttcctgtctg tataaagacc
     6961 gtctatatca gtgtatttag ctttcttcat aactctgccc ttatcttctt ccgttacata
     7021 tctgatatta tcggtatgag attgaaatgt tttgacaata tcctctaaat taatatttag
     7081 gcattcagac aatatataat aattttccct tacatcttct tcatcatcgc ctgtattaaa
     7141 acctaaattc atggattcaa aatcaccttt gctgactccg ccttttcttg tggtaaagca
     7201 gtgaaatagt tcatcatatc catcaaaaat cggaaaagtc aaatattcaa aatcttttat
     7261 tttatttaat ttatacataa taattcctct cttcaagttc attatggaat tcatatacta
     7321 tgtaaaataa taaaatatcc gctctcggtt tcggccacga ataattctaa agctcattcc
     7381 ggctaa
//
LOCUS       DAOSHP010000085         7099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_9211_length_7099_cov_6.535775, whole genome shotgun sequence.
ACCESSION   DAOSHP010000085 DAOSHP010000000
VERSION     DAOSHP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9211_length_7099_cov_6.535775"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1708
                     /locus_tag="PLB33_07795"
     CDS             326..1708
                     /locus_tag="PLB33_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOG63464.1"
                     /translation="MDVFLYFGMLLALTLIIAAGIYSGKKVKVSSDFSVGSRKAGYMI
                     VAGTITGTLVGGASTVGTSELAFTYGFSAWWFTLGAGIGCLLLGLVFVESIYRNGNET
                     VSQILADEYGNKVGLVSSVFVSIGMFINIVAQILSATAIIVSVFNITSFTASCIAALL
                     MIIYVVFGGVWSTGIVGIVKLLLLYISVIIGGSMALKLGGGISHFIEVLPSEQYFNLF
                     ARGKSIDLGAGFSLIVGVLSTQTYIQAVLSGKDKSASQKGALLSAVLIPPIGLAGIFI
                     GMYMKLNYPDIAPSSTLPHFVLNYMPPFVSGIIMAALLVTVVGTGAGLALGISTVITK
                     DVYKNYIKRNSDELTLLNISRLLIVVVLAAAVIFTAGNMKSYILEWSFMSMGLRGAAV
                     FVPLCSAFFLKGRITSRFALAGVLLSPVSVLIGKTVMHTEIDSLFIGIAVNVIIFTTG
                     ILSNKKADIN"
     gene            1805..3712
                     /locus_tag="PLB33_07800"
     CDS             1805..3712
                     /locus_tag="PLB33_07800"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOG63465.1"
                     /translation="MQNRILLVSSNDELTKLAEKVSKNLNINYDLYEGGITKDGHIHA
                     KNNSHKYDVIISSGGTAEAIRKIVSKPVVSVEISAADILYAIYKAKKFKNKIGIVVYK
                     NDELAQFYKLKNFLNLDCEIFEYETKSEIINKMADATSLGRLTLVGIGGCIKETAIDH
                     NLDYVIIESSEKSIRDALINAANICELTKNDKERAKRYQAILDYSGEGIISYDKNNKV
                     TSINAAAEKILDVKYNDVFNKSIEAINDNFKVLLGDGNKLDNKLTKINGKQVLVNRIP
                     IIIDNDTLSVVVNIQETSNIQKIERNLRAELYRKGLVAKHTFSDIIGNSEIMKDTINK
                     AKRIGKTESTILITSETGCGKELFAHSMHNISSRNGGPFVAVNCAALPENLLESELFG
                     YEEGAFTGAKKGGKIGMFELAHKGTIFLDEISEIPLDLQGRLLRVLQEREIQRVGGDS
                     VVNVDIRIIVASNKNLFEKVINGSFREDLFFRINVLDLYIPPIRERKQDIPLLLDNCF
                     KTIGSRIRVNNFSREALNWIMKYDWKGNVREIENFAQRMFVLLEDCADINENSLENII
                     NYKLISSLSTKYSLENKDNIIQIEIGNIKEMESKIILEAAKKYSGDKTMLADKLGISR
                     TTLWKKLKEAD"
     gene            3986..5332
                     /locus_tag="PLB33_07805"
     CDS             3986..5332
                     /locus_tag="PLB33_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HOG63466.1"
                     /translation="MVLVMAVLTYIIPAGEFVRVEDAATGRTVIDPTSYHAVDQNPTT
                     FFELWKAVPRGMVDAASIIFFMFIISGSFGVISSTGAIEAFISRVALMLKGKDKIMVP
                     MIVFIFSIFGGTIGMAEETIIFIPLGIVLARALGYDAIVGTSMILLGCACGFTSGFMN
                     SFTVGVAQGLAQLPLFSGIGLRIVIWFVFVIFTSVFILRYGAKIKADPSKSMVYELEV
                     AEKDTIVDLSNLTEMKREHYFVMITMIIGFIAIIFGVFKEGWYITEISAAFLAMGVIS
                     GLLGKMGPSKIAQQFIIGAKDIVFGALVVGIARGILMVMKDGVIIDTIVNTLAMIIQT
                     LPRSISVLGMYIVQVILSIIIPSGSGLAATTMPIMVPLSDVLEINRQVAVLTYQFGDG
                     ISNSFIPTAASCMGALSVAKISYEKWLKWSVPLIIGWLILGGIFCMIGMAINYGPF"
     gene            5389..6576
                     /locus_tag="PLB33_07810"
     CDS             5389..6576
                     /locus_tag="PLB33_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOG63467.1"
                     /translation="MMEYTESMNLTEAVKLTQELVRIPSHKDVEDRESAVAEFIYNYC
                     KENGLEVEYQVVEGKRRNVIARLRGNGTGKNLMFSGHIDTVPPYDMEYDDPYCADIVD
                     GYLLGRGCNDMKGAVACMITAMVNIKNKGKVLNGDIILAAVVGEEEKSDGTAFVVKSG
                     ITADGAIEGEPSNYGYSLGHRGLEWIEIKFIGKRAHGCTPELGINAISKASKFIQKIE
                     TELMPKLKERKNEWMGPDIMNFGYIHGGAAPSFVADNCVLQFDRRYTPGETVDDVIKD
                     YQDIIDELKAEDSQFNAEIIRMEENLMDDFDHAPLIAEPDSQIAKTVYKAIKEFIGKE
                     PEIVQRGWTDAGLLSTYGKIPTVVTGPGNVKWSHAKDEKIPVADLVNYVEIYTNIAEQ
                     FCI"
     gene            6771..6959
                     /locus_tag="PLB33_07815"
     CDS             6771..6959
                     /locus_tag="PLB33_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOG63468.1"
                     /translation="MQGKVQKMDHHNPGIKCVVNTCYYYSDGDHCNAQKIEVQKRDAS
                     TSKETDCATFTNNHQSMS"
BASE COUNT         2508 a          949 c         1457 g         2185 t
ORIGIN      
        1 gaaaatatgt ttttctttca tttcttgcct cctcaattat attttcatta tatgaggggt
       61 ttttaaactt gttaaattaa attctatatt tacaaattta atggcacgct aattgcaaat
      121 ataattttaa aataaataat tttatatttt tgtctttaat gtctgttata tggattatta
      181 ttgttaatcg aataaagtgt tttttaatta ttttatattt gcagtcgtat taagcatttt
      241 atgtagtaaa agttaacata atcagttaac ataatgaaca aaacagtata atatctgaaa
      301 atcaattatc ttatggagac aaattatgga tgtgttttta tattttggga tgttattagc
      361 tttaacactg attatagctg cagggatata ttcagggaaa aaagtaaaag tttcgtctga
      421 tttttctgtt ggcagccgaa aagcaggata tatgattgtt gcaggaacta ttaccgggac
      481 tcttgtcgga ggtgcttcaa cagttggaac gtctgaatta gcatttacct acggattctc
      541 tgcatggtgg tttaccttgg gagcaggaat aggatgtttg ttgttaggtc ttgtatttgt
      601 tgaatctata tatcgaaacg gtaatgaaac agtttctcaa atattagctg atgaatacgg
      661 aaataaggta ggtctggttt catcagtctt tgtatcgatt ggtatgttta ttaatattgt
      721 agctcaaata ctttcagcaa cagcaattat cgtctctgtg tttaatatta caagctttac
      781 agcttcatgt attgccgcct tattgatgat tatttatgtt gtgtttggcg gtgtatggag
      841 tacaggcatt gttggaattg tgaaactgct gctattgtac atatctgtaa taataggagg
      901 cagcatggct ttaaaattag gtggtggaat ttcgcatttt attgaagtgc ttccatcaga
      961 gcaatatttt aacctgtttg ccagaggtaa gagcattgat ttgggggcag gattttcatt
     1021 gatagtgggt gtattgtcaa ctcaaacata catacaggct gttctaagcg gaaaagataa
     1081 atcagcttca caaaaaggag cattgctaag tgctgtgctt atacctccca taggcttggc
     1141 aggaatattt ataggaatgt atatgaagtt gaattaccct gatatagcac catcatccac
     1201 attacctcat tttgtcctga attatatgcc gccatttgtt tcaggaatta taatggctgc
     1261 tttgctggta acggttgtag gtaccggagc aggattggcg ttaggaataa gtactgttat
     1321 tactaaggat gtttataaaa actatataaa aagaaattca gatgaattaa cgcttttaaa
     1381 tatttcaaga ttattgattg ttgttgttct tgcagcagct gtgatattta ctgcaggaaa
     1441 tatgaaatcc tacatattgg aatggagttt tatgtcaatg ggattaagag gtgcagctgt
     1501 ttttgtaccc ttgtgcagcg ccttcttcct gaagggccgg ataaccagtc gatttgcttt
     1561 ggctggcgtc ttattaagtc ctgtttcggt tttaatcgga aaaactgtca tgcatacaga
     1621 aatagactca ttatttattg gtattgcagt taatgtcata atttttacga caggaattct
     1681 gtcgaataaa aaagcagaca taaactgaat ttttggttat actttgtaaa gtttatcaat
     1741 aaatatttgc attttattat atttcaccat ataattacat aacaatatat aagaggatag
     1801 aattatgcag aacagaatat tattggtttc atcaaatgat gagcttacaa aattagctga
     1861 gaaagtatcc aaaaatttaa atataaatta tgacttgtat gaaggcggaa ttacaaagga
     1921 tgggcatatt catgccaaga ataactccca caaatatgat gtaattatca gcagcggagg
     1981 cacagctgaa gctatccgaa aaattgtaag caagcctgtt gtttcggtgg aaataagtgc
     2041 tgcagatatt ctatatgcta tttataaagc gaaaaaattc aaaaataaaa ttggtatcgt
     2101 agtatataaa aatgatgaac ttgctcagtt ttacaagttg aaaaattttt taaatttaga
     2161 ctgtgaaata tttgaatatg aaactaaaag cgaaataata aataagatgg ctgatgccac
     2221 atcattagga aggcttacat tggtaggaat aggaggatgt attaaggaaa cagccatcga
     2281 tcacaatttg gattatgtca tcattgaaag cagtgaaaaa agcataaggg acgctcttat
     2341 aaatgctgca aatatatgcg aactgacaaa aaacgataag gaaagagcaa aaagatatca
     2401 agcaattctt gattattcag gagaaggaat aatttcttac gataaaaata acaaagtaac
     2461 atccataaat gcagctgcgg aaaagatatt ggatgtaaaa tataatgatg tattcaataa
     2521 gagcattgag gcaataaacg ataatttcaa agtgcttctt ggagatggaa acaaattaga
     2581 taataagctt acaaaaataa acggcaaaca ggttcttgtc aacagaatac ccataattat
     2641 tgacaatgac actcttagtg ttgtagtaaa tatccaggaa acctccaata ttcagaaaat
     2701 cgagagaaat ttgagagcag agctttaccg caagggactt gtggctaagc acacattttc
     2761 tgatataatc ggcaatagtg aaattatgaa agacactatt aataaagcta agagaattgg
     2821 gaaaacagaa agtactattt taattacttc tgaaacaggc tgtggcaaag agctgtttgc
     2881 acatagtatg cataatataa gcagcagaaa cggaggtcct tttgtagcag taaattgtgc
     2941 tgcacttcct gaaaatcttt tggaaagtga acttttcggc tatgaagaag gggcttttac
     3001 aggtgcaaaa aaaggcggta agatcggaat gttcgaactt gcccacaagg gaactatttt
     3061 tcttgatgag ataagtgaaa ttcctttgga tcttcaaggg agattattaa gagttttgca
     3121 ggaaagagaa atacaaagag tcggcggtga ttctgtagtt aatgtggata taagaataat
     3181 agttgcttcg aataaaaatt tatttgaaaa ggttattaat ggtagcttta gggaggactt
     3241 gttttttaga atcaatgtcc ttgatttgta tatacctcct ataagagaaa gaaagcaaga
     3301 catacctcta cttttggata attgttttaa aacaatagga agcagaataa gagttaataa
     3361 ttttagcagg gaagcattaa attggattat gaaatatgac tggaagggaa atgttaggga
     3421 aatagagaac tttgctcaaa gaatgtttgt attgcttgaa gactgcgcag atataaatga
     3481 aaatagctta gaaaatatta taaattataa attaataagt agtttaagca cgaaatactc
     3541 tcttgaaaac aaggataata tcatacaaat agaaataggt aatattaaag aaatggagag
     3601 taagataatt ttggaagcag ccaaaaagta tagcggtgac aaaactatgt tagctgataa
     3661 acttggcata agtagaacaa ccttgtggaa aaaactaaaa gaggcggatt gacttgttaa
     3721 aaaaataaac aaatataaaa ataattaaca agaataaata acacgaaagt ttttgactag
     3781 agaaatcaag taattgtaac aaaaattgct tgattttttt ttatgcagtt ggcacgtcaa
     3841 ttgcaataac ctttatagat aaatcttaat agataaatcg aaataaatat taatattaaa
     3901 aggaggcaat atgtcagagc aaggtgcaaa aattaaagaa aaaaagaaat tcaaggtacc
     3961 gcatacttag gtaattatat tttccattgt tttagtcatg gctgtgctga cctatataat
     4021 tcccgcaggc gaatttgtca gagttgaaga cgcagcaaca ggaaggactg taattgaccc
     4081 tacttcttat catgctgttg accagaatcc cacaactttc ttcgaactat ggaaggcagt
     4141 accaagaggt atggtggatg cggcgtcaat aatatttttc atgtttatta tttctggttc
     4201 attcggcgtt atttcatcaa caggagcaat tgaagccttc attagcaggg tggcattgat
     4261 gcttaaaggc aaagacaaaa tcatggtacc gatgattgtc ttcattttct caatattcgg
     4321 cggtacgata ggtatggctg aagaaacaat aatatttatt ccgttaggaa ttgttttagc
     4381 gagagcctta ggctatgatg caatagtagg aacatccatg attcttttag gctgtgcatg
     4441 cggttttaca tccggattca tgaattcatt tacagtaggt gttgcacaag gactggcaca
     4501 actgcctttg ttttcgggaa ttggattaag aattgttatt tggtttgtat ttgttatatt
     4561 cacaagtgta ttcattttaa gatatggagc caaaataaag gcagatccaa gtaagagcat
     4621 ggtatatgag ttagaggtag ctgagaagga tactatagtt gacttaagta atttgacaga
     4681 aatgaaaaga gagcattatt tcgtaatgat aacaatgata attgggttca ttgcaataat
     4741 attcggtgta tttaaagaag gatggtacat aactgaaatt tcagctgcat ttttggcaat
     4801 gggagttatc agtgggcttt tggggaaaat gggtcctagt aaaatagcac aacaatttat
     4861 tattggcgct aaagatattg tgtttggagc attagtagta ggtatagcaa gaggcatcct
     4921 gatggttatg aaggatggtg taataattga tacaatagtt aacacacttg caatgataat
     4981 acagacactt ccaagatcaa tatctgtact aggaatgtac atagtgcagg taattttaag
     5041 tataatcata ccgtccggaa gcggtcttgc tgcaacaact atgccaataa tggtaccatt
     5101 gtcagatgtt ttggaaataa acagacaagt tgccgtatta acttatcagt ttggtgatgg
     5161 tatatcaaat tctttcatac ctactgccgc atcatgtatg ggtgcattgt cagtagctaa
     5221 gatttcttat gaaaaatggt taaaatggtc agtgccatta ataataggct ggttgatttt
     5281 aggaggtata ttctgtatga taggaatggc aataaactac gggccattct aagaaaaaaa
     5341 tcacattatt aaactatttg attatcatat ttttattaag gaggaattat gatggaatac
     5401 actgagagta tgaatttaac tgaagctgtg aaactaactc aggaactggt aagaatacca
     5461 agccataaag acgtagaaga tagggaatca gcagtggcag aattcattta taattattgc
     5521 aaagaaaatg gacttgaagt tgagtatcag gtagttgaag gtaaacgtag aaatgtgatt
     5581 gcaaggctta gaggaaatgg aacaggcaaa aacctaatgt ttagcgggca tattgacaca
     5641 gtaccgccat atgatatgga atatgatgat ccttattgtg ctgatattgt tgacggatat
     5701 ttgcttggaa gaggctgcaa tgacatgaaa ggtgctgttg catgcatgat tacagcaatg
     5761 gttaatatta agaataaagg gaaagtttta aacggtgaca ttattttagc agcagttgta
     5821 ggcgaagaag aaaaaagtga cggaacagca tttgttgtaa aaagcggaat tactgctgac
     5881 ggagctatcg agggagaacc atcaaactac gggtattctt taggacatag aggacttgaa
     5941 tggattgaaa taaagttcat aggaaaaaga gctcacggat gcactccgga gttaggcata
     6001 aatgccatat ctaaggcatc aaaattcatt caaaagatag aaacagagct tatgccaaag
     6061 ttaaaagaaa gaaaaaacga atggatggga ccggatataa tgaattttgg atacattcat
     6121 gggggcgcag cacctagctt tgttgcagat aattgtgttc ttcaatttga cagaagatat
     6181 actcccggag agacagttga tgatgttatt aaggattatc aagatataat cgatgaatta
     6241 aaggctgaag attcacaatt taatgcagaa ataatcagaa tggaagaaaa cctgatggat
     6301 gattttgatc atgcaccatt aatagcagag cctgattcac aaattgcaaa aacagtttac
     6361 aaagctataa aagaatttat aggcaaagag cctgagatag tacaaagagg atggacagat
     6421 gccggacttc tttcaacata tggaaagatt cccactgttg ttacaggtcc gggtaatgtt
     6481 aaatggtctc atgcaaagga tgaaaaaatt cctgtcgctg atttagtaaa ctatgtagaa
     6541 atatatacaa atatagccga gcaattttgt atttaatgta aatgatgatt tattaataaa
     6601 aatgagattc ttaaaactat gcatatatgt ttatgtatag caaatagcta tatttggttt
     6661 tataagaatc tcttttatat tatttagtaa taaatttatt ataaaaagaa ctttcaaggg
     6721 cataatattt ttgaaagtat atctttcaat aatgaataag gagacataat atgcaaggca
     6781 aagtacaaaa aatggaccat cataatcccg ggattaaatg tgttgtgaac acatgttact
     6841 attattccga cggagatcac tgcaacgctc agaaaattga agtgcaaaag cgcgatgctt
     6901 ctacatccaa ggaaacagat tgtgcaacat ttacaaacaa ccatcaaagc atgtcataga
     6961 tgacgatgat ctaacccaag acttcgaccg atagggagaa agaaattgat ggtatgtcaa
     7021 atgcacaaaa ttcctaattt atgcgatgca ggagcaaata gaccagtgaa tttacctgca
     7081 agccctaatc gagactgca
//
LOCUS       DAOSHP010000086         7097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_9217_length_7097_cov_8.033797, whole genome shotgun sequence.
ACCESSION   DAOSHP010000086 DAOSHP010000000
VERSION     DAOSHP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9217_length_7097_cov_8.033797"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..565
                     /locus_tag="PLB33_07820"
     CDS             137..565
                     /locus_tag="PLB33_07820"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG63469.1"
                     /translation="MLNKGFTLIELVVAISILCIVSMLAVAIIDIDMFYLEKMADEFA
                     MDVRYVQMECMKGETDNHRICIDITNGCYHIYNVAVIKKTVVFKNRYKIDYSNQNMSV
                     GFTHDGTPVNPGTFTIIDTKTNETLKISIVPTTGRTVIKE"
     gene            608..1165
                     /gene="efp"
                     /locus_tag="PLB33_07825"
     CDS             608..1165
                     /gene="efp"
                     /locus_tag="PLB33_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOG63470.1"
                     /translation="MITAGDFKKGITLSWNNGLWQVVDFQHVKPGKGAAFVRAKLKNI
                     VTGTIKEETFNPTEKFQLAHIETKDMQYLYNDGQLYYFMDPETYEQIPLNYEQVEEAI
                     VYLKENDFAQMRFYEGNPFEVSAPNFVELEIVETEPGFKGDTATGANKPATTETGAKI
                     MVPLFVEIGDKVKIDTRTGEYLSRV"
     gene            1177..1548
                     /locus_tag="PLB33_07830"
     CDS             1177..1548
                     /locus_tag="PLB33_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63471.1"
                     /translation="MDFLYEKISYLRGLADGLEVKDDTKEGKLFYALMEVIEEIADSL
                     SDIVEEQDEVNEYLDLLDEDLSVVEEELFGDLELYEDEDDYDYDDDDDYDFDFDEDDE
                     ENYDDEFEIEEHPCDCAKLED"
     gene            complement(1783..2652)
                     /locus_tag="PLB33_07835"
     CDS             complement(1783..2652)
                     /locus_tag="PLB33_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63472.1"
                     /translation="MKEKKYKSNQKKALIAFITIIIIIAAIATVEKLIEPMGYFRPDY
                     DKRDITGILEKEVLSDDDYKELFYQTGLGKTAVDEILKDKSGKEKILEFQKNFFTEYN
                     ISSEKFAVIVNHESLVDEKGKIIYGFDLAPYENGYVLITKATHSLGWRHGHAGIITDA
                     ENEETLEAVLLGQDSMLQNINKWRSFPSFIMLKLKDASQEELDKIAEFAKINLLDVPY
                     GLSVGLTSKKNPEPSNITTTQCAHIVWYPFMQFGYDIDSDGTWLVTPKDIANSDLFEI
                     VQIYGVDPDEIWP"
     gene            3162..5018
                     /locus_tag="PLB33_07840"
     CDS             3162..5018
                     /locus_tag="PLB33_07840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOG63473.1"
                     /translation="MKKIKGLELSSQQINAASFDKGNALVLSTAGSGKTTVIISRAGR
                     LLFENKCSKKILTITFSKMAANDMKKRFACIGNCQNYETKTEFSTIHAFSYKIVRDFY
                     RRKGINFGLLTNNYQALSEILKNHYSKSYFSYVSDEEIENLSSAIGYVNNMMIAPEEY
                     KDYGIEIKGFDKLYNDYKMYKSQNRLIDFDDMLDYSYKILSDYANYKEMILNTFEYVQ
                     IDEMQDTSKIQHELIKLITRDNLFMVGDDDQAIYSFRGSYPDFMLKFKDIFNDGKIFY
                     LDNNYRSDKYIVEGARNFIEINKNRYKKAIKTENSKENEINIIKVKDRAEQAKLITDI
                     VAKAKYENKTIGILYRYNMSAMIPANNLYENNIDFYIKEDKTKFFSSAVLYDILAFLN
                     LAINPKDKDSYARIYYKSYTYFTKGMCKMVLESNEDKSVLDILYGYKYFDYNVYDKIK
                     QFKTDIDYLNKLKPSNAIRFIKFDLEYLNYLERMQEEGRYSLSTSLHILEILEEIGSY
                     CSDIKEFLRKINNLRDVITRASENGNALVTLTTIHSAKGLEYDYVYMIDNLNELNIDK
                     NLSVEDYQSKLEEERRIFYVGMTRAKEKLNIIVPGEPSIFVNELIISNKCMH"
     gene            5134..6321
                     /locus_tag="PLB33_07845"
     CDS             5134..6321
                     /locus_tag="PLB33_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HOG63474.1"
                     /translation="MKFYISTLGEFDIKVDGQSILRDSNRMYRIYKLFEYFLTFRNKK
                     LLPETIIDNLLSDSESEDPKNMLRTQIFRLRKIINALIPKGELPEKYMNLNFTNGYYC
                     LEIGENTIVDIDVFENLIQKGDKEREYDIESAIESYQNAISLYKGLYLSDNAYEVWLV
                     PTRNYYQRLFLKTLNKYIDLLKRNEENEKIVNLCEKSLLIEPFEESIHIELIEALLKI
                     GQNKSAMQHYEYALNMLEKELDAKPSAKFINYINIIQNYTSRNNDLDIEAINKSLEEE
                     LPEGAMYCSLEYFKFLFNLQKRKSKRNNEYDYLLSIMINSNNNKSINDVFSVLKKSLR
                     KGDVFTSWNDNQILVMLHNVKENGTETIKERLTDNLINYTRINRNEIRLIFQPVSMEN
                     TIL"
     gene            6529..>7097
                     /locus_tag="PLB33_07850"
     CDS             6529..>7097
                     /locus_tag="PLB33_07850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG63475.1"
                     /translation="MEYPSKYDFYLKFKPVYNKEGYFLGYVLIYVSDFFINDINIGAD
                     LTIGKKFSEIVVDSDILGLKEFYFNIIPKSKMKLELYNEDLKRWYLINAFTDMSNRDE
                     HLIIHYADITEIKQNQQTVSLDNNIYYLNDRERLLYKDKLTGLYNKTYFEEELSRLDT
                     KRQLPISLIMGDINGLKLINDAFGHSMGD"
BASE COUNT         2574 a         1000 c         1341 g         2182 t
ORIGIN      
        1 tgtccgctga gatgacaagg gtgtcatcct gagcgatagc gaaggatctc ataaggcccg
       61 actaaattta tataaaattc attgacaaac acccaaataa tttatagaat aaactatggt
      121 atattttagg taaactatgc taaacaaagg ttttacatta attgaattag ttgttgcaat
      181 ttcaattcta tgcattgtat cgatgttagc cgtggcaatt attgatattg atatgttcta
      241 cttggaaaag atggctgatg aatttgctat ggacgtaaga tatgtgcaga tggaatgcat
      301 gaagggtgaa accgataacc acaggatatg cattgatatt accaatggat gttatcatat
      361 atacaatgtt gctgttataa aaaaaaccgt agtatttaaa aacaggtata aaatagatta
      421 ttcaaatcaa aatatgtctg tcggatttac gcatgatggt acccctgtta atccgggaac
      481 ttttaccata atagatacaa agacaaacga aactttgaaa atatccattg ttcccactac
      541 aggaagaacg gttattaagg aataagcagc aatctgtagg ttataatata cataaggagg
      601 aagcaaattg attacagcgg gcgattttaa aaagggaatt actttatcct ggaataacgg
      661 attgtggcag gttgttgatt tccagcacgt taaaccggga aagggagctg ctttcgttag
      721 ggcaaaatta aaaaatattg tgacagggac tataaaagag gaaactttta acccaaccga
      781 aaaattccaa ttggcgcata ttgaaaccaa ggatatgcaa tatttatata atgacggaca
      841 attgtattat tttatggacc ccgaaacata tgaacaaatt ccgctaaatt atgaacaagt
      901 ggaagaagct attgtatatt tgaaggaaaa tgactttgct caaatgagat tctatgaagg
      961 aaatccgttt gaagtatctg cgcctaactt tgttgagttg gaaattgttg aaacagagcc
     1021 gggctttaag ggtgataccg caacaggagc taataagcct gcaacaactg aaaccggtgc
     1081 aaaaataatg gtgccgcttt ttgttgaaat aggggataaa gttaagattg acaccagaac
     1141 aggtgaatat ttgtcaagag tataaggagg tataagatgg attttttata tgaaaaaata
     1201 tcatatttaa gaggtttggc tgacggcctg gaagtaaagg atgacacaaa agagggcaag
     1261 ttgttttatg ctttgatgga agtaattgaa gaaattgctg attcattgag tgatatagtt
     1321 gaagaacaag atgaggtaaa tgaatatttg gatttactgg atgaagattt gtctgtagtt
     1381 gaagaagaat tattcggcga tttggaatta tatgaagatg aagatgatta tgattacgat
     1441 gacgatgatg attatgactt cgattttgat gaagatgatg aagaaaatta tgatgacgag
     1501 tttgaaattg aggaacatcc ctgcgactgt gctaaactag aagattaata actaatgtac
     1561 ataagaagaa catttcttta gagagatgtt ctttttctta tgagattctt cgagctaaag
     1621 ctctcagaat gacaaaatgt tattctgagc aataacttat agtcatcctg agcggagcga
     1681 aggatcacat gagattcttc gggctaaagc tctcggaatg acaaaatgtt attctgagca
     1741 ataacttata gtcatcctga gcggagcgaa ggacctcata tattatggcc agatttcatc
     1801 ggggtcaacc ccgtatattt gaactatctc aaacaaatca ctgtttgcaa tatcttttgg
     1861 tgtcacaagc caagtaccgt ccgaatcaat atcgtatccg aactgcataa atggatacca
     1921 aacaatgtga gcacactggg tagttgttat attgctcggt tcaggatttt ttttgcttgt
     1981 tagtcctaca gaaagcccgt agggaacgtc taataaattt attttggcaa attcagctat
     2041 tttatctaat tcttcctgag atgcgtcttt taatttaagc attataaaag aagggaaact
     2101 tctccactta ttaatatttt gaagcattga atcctgacct aatagtaccg cttccaaagt
     2161 ttcttcgttt tctgcatctg ttatgattcc tgcatgacca tgcctccagc ctaatgaatg
     2221 ggttgctttt gttatcaata catatccgtt ttcataggga gcaaggtcaa atccgtatat
     2281 tatttttcct ttttcatcaa caagagattc atggtttact attactgcaa atttttcgct
     2341 tgatatattg tattccgtga aaaaattttt ctgaaactcg agtatttttt ccttgccgga
     2401 cttatctttc aaaatttcat caacggctgt tttaccgagg cctgtttgat agaacagctc
     2461 tttgtagtca tcgtcactta atacttcctt ttccaatata ccggtaatat ctcttttatc
     2521 gtaatcagga cgaaaatagc ccattggctc aatgagcttt tcaactgttg ctattgcagc
     2581 aattataata attattgtta taaaagcaat aagtgctttc ttttgatttg atttatactt
     2641 tttttctttc atagtttcta ccattgtttc ttatagtgtc ttacgtggag agtcattctg
     2701 agggctagcc cgaagaatct catgagatcc ttcgcttccg ctcaggatga caaaaccgag
     2761 atccttcgct tccgctcagg atgacaaaac cgagatcctt cgcttccgct caggatgaca
     2821 aagcggattt tactatatta tatagacaat aattaaaaat tacaatggca aaggggcatt
     2881 ttttatgtta caatatgtat ataagtaagt tggaggaatg tacatgaaca aatataaaaa
     2941 agctgataga atgaagagag ttatttgttt aatcttggcc gtaggaatgg ttttaaccgc
     3001 atttatgagt ttattggcat acttttttta aatagtataa ttcttatata tgggacgttt
     3061 aaaaaccgta cccgtcaggt tgcgaagaat tgtcactctg agcaaagcaa acgagcctca
     3121 aacgtctcta attgtggtga tatatggagt actttgatta tttgaaaaaa attaaggggt
     3181 tagaattaag cagccaacag ataaatgccg cctcttttga taaaggaaat gcactggttc
     3241 tttccactgc aggctcaggc aagactaccg ttatcatttc acgggcagga aggcttttat
     3301 ttgagaataa atgcagcaaa aaaattctaa ccattacatt ttctaaaatg gcagccaatg
     3361 acatgaagaa aagatttgca tgtattggaa attgtcaaaa ttatgaaaca aaaactgaat
     3421 tttcaactat tcatgccttt tcttacaaaa ttgtgaggga tttttacagg aggaagggca
     3481 ttaattttgg attgcttacg aataattatc aggccctttc ggaaatactt aaaaatcatt
     3541 attccaaatc atatttcagc tatgtgagtg atgaagaaat tgaaaattta tcttctgcaa
     3601 tcggatatgt gaacaatatg atgattgctc ccgaagaata caaagattat ggaatagaaa
     3661 taaaaggttt tgataagctt tacaatgatt acaaaatgta caagtcacaa aacagattga
     3721 ttgattttga cgacatgctg gactattcat ataaaatatt atcagactat gcaaattaca
     3781 aagagatgat tttaaataca tttgaatatg tgcagattga tgaaatgcag gatacttcta
     3841 aaattcagca cgagctgatt aagcttataa cacgtgacaa tttatttatg gtaggagatg
     3901 atgaccaagc catatattca ttcaggggaa gctatccgga tttcatgctt aagtttaaag
     3961 atatttttaa tgacggtaaa attttttact tggacaacaa ttaccgctca gacaaatata
     4021 ttgtggaagg tgcaaggaat tttattgaaa taaataagaa cagatataaa aaagctataa
     4081 aaacagaaaa ttcaaaagaa aatgaaatta atataatcaa ggttaaagac agagcagagc
     4141 aggctaagtt gattacggat atagttgcaa aagcaaagta tgaaaataag accataggta
     4201 ttttatacag atacaatatg tcggctatga taccggcaaa caatctctat gaaaataata
     4261 ttgattttta tattaaagag gataaaacta agtttttcag cagtgctgta ctttatgata
     4321 ttcttgcatt tttgaatttg gcaataaacc cgaaggacaa ggattcatat gcaaggatat
     4381 attacaaaag ctacacctat tttaccaagg gtatgtgtaa gatggtactg gaaagcaatg
     4441 aagacaagag tgttcttgat attttgtacg ggtacaagta ttttgactat aacgtttatg
     4501 acaaaattaa gcagtttaaa acggatattg attatttaaa caagttgaag ccatccaatg
     4561 cgataagatt tataaaattt gatttggaat atttaaatta tttggagaga atgcaggagg
     4621 aaggacgata cagcttatca acctctcttc acatattgga gattttggag gaaataggct
     4681 catactgcag cgatataaag gaatttttaa gaaaaatcaa taatttgaga gatgtaataa
     4741 ctagagcttc agagaacggc aatgctcttg taacattgac tacaatccac agtgccaagg
     4801 gattggaata tgattatgtt tatatgattg ataatttaaa tgaattaaat attgataaaa
     4861 acttatccgt tgaagactat caaagcaaat tagaggaaga acgcagaatt ttttatgtgg
     4921 gcatgaccag agcaaaagaa aaattaaata taattgttcc aggagaaccg tctatatttg
     4981 taaatgaatt gattatatca aataaatgca tgcattgatt aaacagcttc aaaaaaaata
     5041 ttgaaatatg ctttaattaa gtatataatg ttaataattg acaagggaaa attaacaggg
     5101 tttattagag gggattattc aaggggggaa atcatgaagt tttatattag tactctggga
     5161 gaatttgata taaaggttga cggacaatca atattgagag attcaaatcg catgtacagg
     5221 atatacaagc tatttgagta ttttctaaca ttcagaaata aaaaattatt acctgaaaca
     5281 atcattgaca acctgctgtc cgacagtgaa tcggaagacc ctaagaacat gcttcgaaca
     5341 cagatttttc gccttagaaa gataataaat gctttaattc ctaaaggcga acttccggaa
     5401 aaatatatga atttaaactt tacaaacgga tattactgtc ttgaaatagg tgaaaatacc
     5461 atagttgata ttgacgtatt cgaaaacctt atccaaaaag gggacaagga acgagaatat
     5521 gacattgaaa gtgcaattga gagttatcaa aatgcaatca gcctttataa gggactgtac
     5581 ttatccgata acgcatatga agtatggttg gtgcctacaa gaaattacta tcaaagatta
     5641 tttttaaaaa cattgaataa atatatagat ttattaaaaa gaaatgaaga aaacgaaaaa
     5701 attgtaaatc tatgcgagaa atcgctattg attgagccgt ttgaagaaag tatacatatt
     5761 gaattgattg aagcgcttct taaaataggg cagaacaaat ctgcgatgca gcattacgaa
     5821 tatgcattaa atatgcttga aaaggaattg gatgcaaagc cttccgctaa gttcataaac
     5881 tacataaata taatacaaaa ctacacttca agaaacaatg atttggatat tgaagcaatt
     5941 aataaaagct tggaggaaga gcttccagaa ggcgcaatgt attgcagtct tgagtatttt
     6001 aaattccttt ttaatttaca aaagagaaaa tccaagagga ataatgaata tgattatctt
     6061 ttatcaataa tgattaacag caataataac aaaagtataa atgatgtatt ttccgtatta
     6121 aaaaaatcgt tgaggaaagg tgatgttttc accagctgga acgataatca aattttagta
     6181 atgcttcata atgtgaaaga aaacggtaca gaaacaatta aagaaagact gaccgacaat
     6241 ttaataaatt atacaagaat taacagaaat gaaatcagac tgatttttca acctgtcagc
     6301 atggaaaaca cgatactgta gatgtgttgg aagcaaagca aaaagtccgt tattgtggac
     6361 tttttttatt gcccctgata aaaagtggtg tgtcccagca ccttatgtaa ttaaattgta
     6421 attaaaaagt aatcaatatt aaattgcata ataatagaaa tgtaattcat agagattata
     6481 attaaaataa atatgaagga aaaacgtcta agaaggcgag gagataaagt ggagtaccct
     6541 tcaaaatacg atttttattt aaaatttaaa ccggtataca acaaggaagg ctattttttg
     6601 ggttatgtct tgatatatgt cagtgatttt tttattaatg atatcaatat cggggctgat
     6661 ttgaccatag gcaagaaatt ttccgaaata gttgtagatt cggatatttt aggtcttaag
     6721 gaattttatt ttaacatcat acccaaatct aaaatgaagc ttgaattata caatgaagat
     6781 ttaaaaagat ggtacctcat aaatgccttt acggatatga gcaacaggga tgagcatctt
     6841 ataattcatt atgcggatat aacagagata aaacaaaacc aacaaaccgt atctttagat
     6901 aataatatat attatttaaa tgacagggaa agacttttat ataaggataa gttaaccgga
     6961 ttatacaaca aaacctattt tgaggaagaa ctgtcaaggc ttgacaccaa aagacagctt
     7021 cctataagtc tgataatggg ggatataaac ggactcaaac ttatcaatga tgcatttggt
     7081 cacagcatgg gtgacag
//
LOCUS       DAOSHP010000087         7090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_9225_length_7090_cov_6.158778, whole genome shotgun sequence.
ACCESSION   DAOSHP010000087 DAOSHP010000000
VERSION     DAOSHP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9225_length_7090_cov_6.158778"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..774)
                     /locus_tag="PLB33_07855"
     CDS             complement(217..774)
                     /locus_tag="PLB33_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOG63476.1"
                     /translation="MIMEHSILIAGSGGQGIMLIGKLLSYAAIEAGKEATFYPAYGAE
                     MRGGTANCTVVISDEEISSPILERFTTIIALNEPSYIKFHSQVIDGGNILINSSLIAS
                     ISEELGKKASVYAVPVNNIALELGSEKISNIVLIGAYIGITGIIDPEVFRNTIRKMLY
                     KDEELMNLNLIAFDKGLAAGQDFRL"
     gene            complement(771..1517)
                     /locus_tag="PLB33_07860"
     CDS             complement(771..1517)
                     /locus_tag="PLB33_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOG63477.1"
                     /translation="MNKVYEKPKLIPADLSNSWCPGCGHGTIHKLIANTIDTYRMKEE
                     TIMISPVGCGTMNTATGWFDCNSVACAHGRASAAAYAIKKCYPDRLVFAYQGDGDLAS
                     IGMAETIHTANRGGNLTIIFVNNGNFGMTGGQMAPTSLIGQRTTTSQEGRNFKDHGNP
                     IGVCELIATLEMPKYVVRVSLHDAKHVIQTQKAIEKAFRMQSEGKGFSFVEVLSPCPT
                     NWKMSPVESMKHINDVVIKTYPLGVFKEDK"
     gene            complement(1510..2592)
                     /gene="vorB"
                     /locus_tag="PLB33_07865"
     CDS             complement(1510..2592)
                     /gene="vorB"
                     /locus_tag="PLB33_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOG63478.1"
                     /translation="MEKIFLKGNDAMAEAAIRAGCRFFAGYPITPQNEIPEYLSKRLP
                     EVNGVFIQGESEIASISMVYGVASTGIRSMTSSSGPGLCLKTEGINFLVAAEIPAVII
                     DVMRAGPAIGGIQPAQQDYTFITGGAGPGGVRCFVLAPYTVQEAVDLVYEAFDIADKF
                     RNPVIVMVDGAIGNMMENIILPELRELDTLPQHEDWKITKRDADGKARIIQTYIHSVI
                     GLEEANKRMAEKYKIWEDYEQRYEAIMLEDAEIVVTAYGIVARMAKAAVKELRKEGIK
                     VGMIRPIAIYPFPKKAFDSLDYGRVKQIWDIELSIPSQMFRDVDYTVRGRAPVYQLLR
                     SGGMMTSKESIIKAIREKALEGENYE"
     gene            complement(2603..2806)
                     /locus_tag="PLB33_07870"
     CDS             complement(2603..2806)
                     /locus_tag="PLB33_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG63479.1"
                     /translation="MAKIIISENLCKGCSLCVTQCPKNIIKISNKINSKGYTIAEQTD
                     DTQCTGCTLCAIVCPDSAIEVYK"
     gene            complement(2849..4360)
                     /locus_tag="PLB33_07875"
     CDS             complement(2849..4360)
                     /locus_tag="PLB33_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOG63480.1"
                     /translation="MEKQSELSKAKQIDNNERGQWSSKIGFLMASIGSSVGLGNIWGF
                     PYKMGANGGFAFLIIYLALLVFVGFAALLGEFTLGRMTGEGIIGTYRTLNKKFTFVGW
                     FGVISAFLVISFYCVLGGFVLRYAVGFLMEIFSAGSGFGGLGSGEFFGSFIVNGFSSA
                     FYFFIFLGLTSVIVMGGVKGGLEKFTTFAMPTLSIIMIIVIIKSLTLEGAIEGVKFMF
                     EPNWQAFSEIGFVKILKTAAGQMFFSLSLGMGIMVIYGSYLSKQEDIQKSSIVIGVAD
                     TIFAVLAGLMIMPAVGAFMAQGVEGITYGAGPGLLFITMHQVFSVGMGGFMGNLFGFM
                     FYMLVFFAAITSSISVLEVCIAFFVDKRLKEGKSSQRIKITAIVCIVIFILGLPVTLD
                     GLGSGTAGGAAIDIPAVMFGMSEVRVAFDCWLDFYDMISEGLLMPLGTMLMCLFIGWI
                     IGTKAIVKELEETPKVKIISYRFWEICFKYIAPVIVFIVFLGQIDDFFGLGIF"
     gene            4681..5379
                     /locus_tag="PLB33_07880"
     CDS             4681..5379
                     /locus_tag="PLB33_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOG63481.1"
                     /translation="MNKKTLELYIESNPFEQLNKIVFYMLLKEISSLKYKPGQKLNVS
                     QISSDLQISRTPITEALKMLVEYGLVKDNLDKQGYYVSVLSRKEAIDIHKARKALESM
                     AAFICAENGSCPNIDKMEELAYRFVDFFKKEKYEIVDAVDVPFHKLIIESSGNKYIQR
                     CYKLLERQLYRYQCYSMSNVMNDNINPISNELEYQHISIVNAIKLHLPNLARQSMEQH
                     IDSCMMLLNGYMRN"
     gene            complement(5486..6118)
                     /locus_tag="PLB33_07885"
     CDS             complement(5486..6118)
                     /locus_tag="PLB33_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOG63482.1"
                     /translation="MIDTVIFDFDGTLADTNNLVLNSFKHIYNKYYDGKWDEEYVLST
                     FGEPLALTLKRDFSGFAYEEVLASYREYQADRFEKEVTLYDTVSETVKYLHKKDIKIG
                     IATSRIKPPTLFALKNFDLDKYFQVVISAEDVEKHKPDKEPLIKAIQGLDSSAENTLY
                     VGDSKFDMECALNAEVTPVLVGWQKNSGELAEKYKIKHVLSKMWDLTWLI"
     gene            complement(6111..>7090)
                     /locus_tag="PLB33_07890"
     CDS             complement(6111..>7090)
                     /locus_tag="PLB33_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOG63483.1"
                     /translation="LQDPLAELVKIDPKSIGVGQYQHDVNQTKLGQELDGIVEDCVNS
                     VGVDLNTASPALLQYVSGITKTISKNLVKYREENGKFVNRQELMKIKMLGGKAFEQCA
                     GFLRISDGENPLDKTAVHPEAYEIAEKLLKKLGYTINDLLKGNLRDINERIMKIPVKE
                     DKKAKMPAKNVRLKGFEELAQISFKEDKKPSKEKIKDRIKVLSEELNVGVPTLTDIIE
                     ELKKPGRDPRDEMPKAVFRSDVVSFEDMRVGMMLTGTVRNVVDFGAFVDIGVKQDGLI
                     HLSEMSDKYIKNPMEVVQVGDTVNVTIISIDKERQRIGLSMKKSRSKND"
BASE COUNT         2164 a         1506 c         1073 g         2347 t
ORIGIN      
        1 tatagtgcat attctttcat ttttttcgac aatcaaattt ttaaattcca taactcctcc
       61 tataaattct tttcagcttt attatagcat ttaaaggaag atttttcatt aatttattca
      121 tataaaatta aagcaaaaaa caagtaccca atatgacaga tgtatatgtt ctaccgggta
      181 cttcaataag tatatctgaa cagctgattt taacagttat aaacgaaagt cctgacctgc
      241 cgccaaccct ttatcaaaag caattaaatt caggttcata agttcttcat ctttatataa
      301 catttttctt atagtatttc tgaaaacctc cggatctatt attccggtaa ttcctatata
      361 ggcaccaatc aaaactatgt ttgagatttt ctcgctgcct agctcaagtg caatattatt
      421 gacagggaca gcataaacag atgctttttt ccccagttct tcagaaatcg aagctatcaa
      481 tgacgaatta ataagtatat ttcctccatc aattacctgt gagtggaatt ttatataact
      541 tggttcatta agggcaataa tcgtagtgaa acgctctaaa ataggagagg atatctcttc
      601 gtctgaaatt acaactgtac aattcgcagt accacctctc atttcagcgc cataagcagg
      661 ataaaatgtc gcttccttac cagcttcaat tgctgcatag cttagcaact tgcctattaa
      721 catgattccc tggccgcctg atccggcgat tagtatagag tgttccataa tcatttatcc
      781 tccttaaata cacctaaagg gtaagtctta ataacgacat cattaatatg tttcattgat
      841 tccaccggac tcattttcca atttgtagga caaggtgaaa gaacctcgac aaatgaaaat
      901 cccttgcctt cactctgcat tcgaaaagct ttttctattg ccttttgtgt ttgtattaca
      961 tgctttgcat catgcagaga aacccgcaca acatatttag gcatttccaa agtagcaatt
     1021 aattcacata caccaatcgg gttgccatgg tctttaaaat ttcttccttc ttgcgatgta
     1081 gtcgtcctct ggccaattaa gctggtaggt gccatttgac cgccggtcat tccaaaattt
     1141 ccgttattca caaaaattat cgttagatta cctccacggt tagcagtgtg aattgtttct
     1201 gccatgccta tggaagcaag atccccatct ccctgatacg caaaaaccag tctgtcaggg
     1261 taacactttt ttatggcgta agcagcagca gaagcacggc catgtgcgca agcaactgag
     1321 ttgcaatcaa accaaccagt tgcagtattc attgtgccgc atcccacagg acttatcatg
     1381 attgtctctt ctttcatgcg ataagtatcg atagtatttg caatcaattt gtgtatagtt
     1441 ccgtgaccgc aaccggggca ccaggaatta cttaaatctg caggtattaa tttgggtttt
     1501 tcatatactt tattcataat tctctccttc cagtgccttt tcccttatag ccttaattat
     1561 gctttcttta cttgtcatca ttccacctga tcttaacagt tgataaactg gtgctcttcc
     1621 gcgaactgta taatctacat cccggaacat ttgcgatggt atggaaagtt ctatatccca
     1681 aatttgttta accctgccat aatccaatga atcaaacgct ttcttaggga atggatatat
     1741 tgcaatcgga cgtatcatcc ctactttgat gccttctttt cttaattctt taacagcagc
     1801 ttttgccatg cgggctacta ttccatatgc tgtaacaaca atttcagcat cctccaacat
     1861 tattgcttca tacctttgtt cataatcctc ccaaattttg tatttctctg ccatgcgttt
     1921 atttgcttct tccaatccga ttactgaatg aatgtacgtt tgtattattc tagctttacc
     1981 atctgcatcc cttttagtaa ttttccaatc ctcatgttgc ggcaaagtat caagctccct
     2041 caattcggga agaattatat tttccatcat atttccaatc gcgccatcca ccattacaat
     2101 aaccggattg cggaatttat cagctatgtc aaaggcttca tagacaaggt ctactgcttc
     2161 ttgcacggta tatggggcaa gaacaaaaca tcgtacacct ccgggtcctg cccctccggt
     2221 aataaatgtg taatcttgtt gtgctggttg aattcctcca attgccgggc cggccctcat
     2281 aacatcaatt atcacggctg gtatttcagc tgccactaaa aaattaatac cttcagtttt
     2341 taaacaaagt cctggtcctg atgaagatgt catgcttctg ataccggtag atgcaacacc
     2401 atataccata gaaattgatg caatttcgct ttctccctga ataaatactc cattaacttc
     2461 cggaagtctt ttagaaagat attcaggaat ttcattttgt ggtgtaatag gatatccggc
     2521 aaaaaaacgg catccagctc ttattgctgc ttctgccatt gcatcgtttc cctttagaaa
     2581 aattttttcc atcttcatcc tcctatttat aaacttcaat agctgagtcg ggacacacta
     2641 tagcacatag tgtacaacct gtgcattgtg tatcgtctgt ctgttctgct attgtataac
     2701 cctttgaatt gattttattg ctgattttta tgatattctt cggacattgg gtcacacaga
     2761 gactgcatcc cttacataaa ttttctgaaa ttataatttt agccatacag gttctcctta
     2821 ttaagagttt tataaaaaaa ccagtttttt agaatattcc taatccaaag aaatcatcta
     2881 tctgccccaa aaatacgatg aaaacaatta ccggtgcaat atacttaaag caaatttccc
     2941 agaaacgata actaattatt tttactttag gagtctcttc taactctttt actattgcct
     3001 ttgttcctat aatccacccg ataaacaaac acatcagcat tgtgcctaac ggcataagca
     3061 aaccttcact aatcatatca taaaaatcca gccagcagtc gaaagcaacc ctgacctcac
     3121 tcataccaaa catgacagca ggaatatcaa tagctgcgcc tccggctgta cctgagccaa
     3181 gaccatccag cgtaaccggt aaccccagaa tgaatataac tatacaaacg atagcagtta
     3241 tcttgattct ttggctgctt tttccttctt ttaaacgttt atctacaaaa aaagctatac
     3301 atacctcaag cacagaaata gatgaggtaa tggctgcaaa aaatacaagc atgtaaaaca
     3361 taaatccaaa aagatttccc atgaaacctc ccatccccac actaaatact tgatgcattg
     3421 taataaacag taacccagga cctgcaccgt atgtgatacc ctcaacacct tgtgccataa
     3481 aagcccctac tgcaggcatt atcattagtc ctgcaagcac tgcaaatata gtgtctgcca
     3541 ctccaataac aatagaactt ttctgaatat cttcttgttt gcttaaatat gacccatata
     3601 taaccattat acccattcca agagacagtg agaaaaacat ttgtccggct gctgttttca
     3661 gaattttaac aaaacctatt tccgaaaacg cttgccaatt tggttcgaac ataaatttta
     3721 ctccttcaat tgcaccctca agagttaggc ttttaattat tacaatgatc ataataatag
     3781 aaagggttgg cattgcaaat gtcgtgaact tttctaatcc tccttttact ccgcccatta
     3841 caataactga tgttaatccc agaaatatga agaaataaaa agcgctggaa aagccattca
     3901 caataaagga accaaaaaat tcaccgctgc ctaagccacc aaatccagaa ccggcactaa
     3961 atatttccat caaaaatcca actgcatatc gtaatacaaa tccacccagg acacaataga
     4021 aggatataac taaaaacgct gaaatcactc cgaaccagcc gacaaaagtg aattttttgt
     4081 ttaaagttct gtatgtgccg ataatacctt cacccgtcat tcttcccagt gtaaattcac
     4141 ctaataacgc tgcaaaacca acaaaaacta atagcgctaa atagatgatc agaaaagcaa
     4201 acccgccgtt tgcacccatc ttataaggaa atccccaaat gtttcccaaa ccaacagatg
     4261 aaccgatgga tgccattaga aaacctattt ttgaactcca ttgacctctt tcattgttgt
     4321 caatttgttt tgcttttgat aactcagatt gtttttccat aaataaatcg tcctcctatt
     4381 agattatatt agtacataaa tacataaata catattaaca actaacattc actgtgtcaa
     4441 aattcaaacg tttttctgca taaatactta aataatacta tattaagcac aatatattag
     4501 aaaaaatagt ttttctgcat ttattaaaaa agttttctgt gtacaactta ttttaattgt
     4561 gttataataa aaaaactaca taaaaattgt cataaccggc acttgataat ttttaaactg
     4621 ttatatatat aaaacagttc aattttacca ttcaatctaa gattagacag gagtaatatt
     4681 atgaataaaa aaacattaga actatatatt gagtcaaatc cttttgaaca gcttaataag
     4741 attgttttct acatgttatt aaaagaaatt tcttccctga aatataagcc ggggcaaaaa
     4801 ttgaatgttt cacaaatctc ttctgatttg cagataagtc gcactcctat aaccgaggct
     4861 ttgaaaatgt tggtggaata cggattagtt aaagataatc tggataaaca agggtattat
     4921 gtttctgttt taagcaggaa agaggcaatc gacatacata aagcacgaaa agcacttgaa
     4981 agcatggctg catttatatg tgcagaaaac ggcagctgtc cgaatattga taaaatggaa
     5041 gaattagctt ataggtttgt tgattttttt aaaaaggaaa aatatgagat agtagatgca
     5101 gtagatgtcc cgtttcacaa gttaattatt gaatcaagcg gaaataagta tatacagcga
     5161 tgctataaat tattggagcg tcagctttat cgataccagt gttattctat gtctaacgta
     5221 atgaacgaca atattaatcc aatctctaat gaattagaat atcagcatat ttcaatagtt
     5281 aatgctatta agcttcatct ccctaattta gccaggcaat ctatggagca gcatattgat
     5341 tcctgcatga tgcttttaaa tggatacatg cgaaattaat acaaagataa ctgaagatac
     5401 ttttaattta aaaaacaaaa aaatgctcca atcatgttga tattacataa ttggagatat
     5461 atattaagta aatagtttat aaaagctata ttaaccaggt taaatcccac attttactga
     5521 gcacgtgctt aatcttgtat ttttccgcca attcaccgga gtttttctgc caccccacaa
     5581 ggacaggagt aacttctgca ttcagggcac attccatgtc aaatttagag tcgcctacat
     5641 acaaggtatt ttccgcactg ctgtctaatc cctggatggc ctttatcaag ggctctttat
     5701 caggcttgtg tttttctaca tcttcagcag aaataacaac ttgaaaatat ttgtcaagat
     5761 cgaaattttt taaggcaaat aaggtgggag gtttaatcct tgatgtggct attccgattt
     5821 ttatatcctt tttgtgcaga tactttacag tctcactgac agtgtcatat agggttactt
     5881 ctttttcaaa cctgtcagcc tgatactctc tgtaggatgc taaaacttct tcataggcga
     5941 agccgctgaa atcccgtttt aaagttaatg caagaggttc tccgaatgtg cttaaaacat
     6001 attcttcatc ccatttacca tcataatatt tgttgtaaat atgcttaaaa gagtttagta
     6061 ccaaattatt tgtatctgcc agtgttccgt caaaatcaaa tattaccgtg tcaatcattt
     6121 ttactcctgc tttttttcat gcttagacct attctttgtc tttctttatc tatgcttata
     6181 attgttacat tgacagtatc tcctacctga accacttcca tgggattttt gatatatttg
     6241 tcgctcattt cagaaagatg gatcaaaccg tcctgtttaa ctcctatatc aacaaatgca
     6301 ccgaagtcaa ccacgtttct cactgttcct gtaagcatca ttcctaccct catatcttca
     6361 aagctcacaa catcgctcct gaatactgcc ttgggcattt cgtcacgtgg gtctcttccg
     6421 ggttttttaa gctcttctat aatatctgtc aatgtaggta ctcccacatt taattcttct
     6481 gacaaaactt ttattctgtc tttgattttt tcctttgaag gctttttgtc ttccttgaag
     6541 cttatttgag ctaattcttc aaagcctttc agtctgacat ttttagcagg catttttgct
     6601 ttcttgtctt cttttacggg aattttcatt attctttcat ttatgtccct taaatttccc
     6661 tttagcaggt catttattgt atagcctaat ttctttagaa gtttttctgc aatttcatat
     6721 gcttcaggat gaactgcagt tttgtcaagg gggttttctc cgtcggagat tcttaaaaat
     6781 cctgcacatt gctcaaaagc ttttccacca agcattttta tcttcatgag ctcttgtctg
     6841 tttacaaatt tcccgttttc ttccctgtat tttactaaat ttttagaaat tgtctttgtt
     6901 attccggata catactgtag cagggccgga gatgctgtgt ttaaatcaac acctacgctg
     6961 ttaacgcagt cttccacaat tccgtcaagc tcctgtccca gctttgtctg gtttacatca
     7021 tgctgatatt gtcccacacc gatgcttttg ggatcaatct taacaagctc ggccaagggg
     7081 tcctgaagtc
//
LOCUS       DAOSHP010000088         7018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_9346_length_7018_cov_8.425822, whole genome shotgun sequence.
ACCESSION   DAOSHP010000088 DAOSHP010000000
VERSION     DAOSHP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9346_length_7018_cov_8.425822"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLB33_07895"
     CDS             <1..326
                     /locus_tag="PLB33_07895"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOG63484.1"
                     /translation="FDITEVDYAILETSGKLTVLKKQKFDPITKKDMPIPVKQNRNLP
                     VQIINNGKIIESVMTEFNLDIDWVYSEAKRLGYKNIKDIFYAGINCDGQFFIVPLFNE
                     KKYKQ"
     gene            complement(336..1304)
                     /locus_tag="PLB33_07900"
     CDS             complement(336..1304)
                     /locus_tag="PLB33_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOG63485.1"
                     /translation="MINISDKIKKKLDMLPMEPGIYKMIDAKGNVIYIGKSKMLNKRV
                     KTYFANNPNWDKINRMVRLIDDIDFIVTDTHLEARILECSLIKELKPIFNLQMKHDRG
                     YVYLKLENYNKNRALSVVESREENCFGPFRKKFHLNETINSLKNIYPIIKKGESYSFE
                     FHLFPLTLNEIEFNKNRYSLYEILTDNSKAGIFIKELENKMKREALLCDFEKASFYKD
                     ILINVRYLINSINKYNELLKSNVIVSIPFDEGYKLFYISKGIILKKEVFKAPNKKDIE
                     IFSKAALTSPDDSFINMDEKTLMDYKDIICSEIISLSDTVVKYMDI"
     gene            complement(1438..1824)
                     /locus_tag="PLB33_07905"
     CDS             complement(1438..1824)
                     /locus_tag="PLB33_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOG63486.1"
                     /translation="MSNDILNILIIEDNKDLVDIMCEMLVLSGYRALSAKNGTEGIAK
                     ARENRPDVIICDIGLPDMSGYEVAKIIRKDSELKDTYLIASSGYAQPEDVERSRETGF
                     NIHLAKPISFNILGKVLKGVKLKCMQ"
     gene            complement(1848..3428)
                     /locus_tag="PLB33_07910"
     CDS             complement(1848..3428)
                     /locus_tag="PLB33_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63487.1"
                     /translation="MSIVNTFIHETDFYRILFENLPQAIQINQIVFNETGEPVDYLLL
                     DVNRTAEKYMGLKREEIVGKRIKEIYSFIEKEWFIRYGEAVKTGKFDRLEIYIEALGI
                     WFDVQVIPLGSQNKFAILFQDITELKNKEASEKKALNDRKKIEIEKSHYMKIAEERAA
                     WLQTIMDLMPAGVWISDYSGKVIMVNQEAINMYRGSSSMTDIPKEYTSYRLFLPDTDE
                     PVTFEPYPLKEALVGVMLDFERFDGTRGTQVASTEALRDKDGNIINYVAVAMDITSLR
                     QAERALAESEKNALDLVEKLRQADHNKNVFINMLSHELRNPLASIIMGLELLEKAPSD
                     GKQAYMAMDIVKRQAKLLTDLVDDLLDVTRITHNKVYLKKDTLEINNLIGKVVQDYQP
                     QFIDKNVKLEFKSTIPVYIEADSSRLAQAIGNLLHNAVKFTNEKDLVIVSAALDTNSS
                     EAVIAVEDTGRGIDQTDQPNLFEPFMQVDKSLDRKLGGLGLGLTIVKRVVELHGGRVE
                     VFSEGVGKGSKFTIRLPI"
     gene            complement(3677..4603)
                     /locus_tag="PLB33_07915"
     CDS             complement(3677..4603)
                     /locus_tag="PLB33_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOG63488.1"
                     /translation="MMKKYLDINPKVKTALEQGKPVVALESTIISHGMPYPKNVETAL
                     NVEKIIRDNGAVPATIAILSGKLKVGLTEKEIEYLGKATNVIKASRRDIPYIVSKKLD
                     GATTVASTMIVASLAGIKVFATGGIGGVHRGATETFDISADLEELANTNVAVVCAGAK
                     SILDIGLTLEYLETHGVPVVGYQTEEMPAFYTRKSGYKVDYKLDTPEEIAKMLKAKWD
                     LGLKGGMVVANPIEEQYQMDYDIITDAINKAVKQADENGIKGKDTTPFLLAKVKEITG
                     GDSLESNIMLVYNNASLGAKIAVELSKIYEIE"
     gene            complement(4658..6325)
                     /locus_tag="PLB33_07920"
     CDS             complement(4658..6325)
                     /locus_tag="PLB33_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOG63489.1"
                     /translation="MKTRIFIILILTLLMTVAPFVTEDVYGAEYVKGQSIVAEDQEKA
                     SRGLEFPGLDITWLDIGDSHIQSYSQMDIFSEGIFTLRLSGQTICFDTTGKIIAVGDY
                     NGIHEFSDGMAKVYKYIPRDEPDVPGRLMAPPGQMEGFIDSKGNEVIPLGMHSNMGDM
                     FHEGFTTIGGYEENKGFINKTGEIVIPQIYKDAGNFSEGLAAVQDIETELWGYINKDG
                     DIVIPMLYDEAKPFREEVAYVVKNGMAGYIDKEGNIIIDFMLMPETDKYVDNSFYGGL
                     AVAWDSSGKYGYINKNGDYVLPVKYKEANPFIGEAVYVVIENQNYPNGYGSSFLINRD
                     GERLTPLWQYGRYAGEYMRDGLIRALSTYGPSPKEFIVMLNKYGAEVIPASLNIQYLS
                     SFNEGYALIIADNNGGTAIGLVELPENIDAYKDRKLIRVFIDDKLLNFEDTDPIIENS
                     RTLVPMRAIFEALGAEVEWDDTNKTAIARKDNTTISLKIDDNTAYINNEPVELDVPAK
                     IKNSRTLVPVRFIAESLNADVKWNDEGRAVLIKTSGIDNTAVDDYQE"
     gene            complement(6362..>7018)
                     /locus_tag="PLB33_07925"
     CDS             complement(6362..>7018)
                     /locus_tag="PLB33_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63490.1"
                     /translation="IANETQPWDESISYTIKDTKWYRGVIYVGATLATFAAFFMISSA
                     QQLPPNKGDINIAEFAENYNYYAKLYKIDFGNEYLDEDGKWAEKETDGTVIYINLTEK
                     PEYKFTIENGYVTGVSFDIEMKNHDYMISSYDKHMFLASLALAGAQNEMGLFSKIPKR
                     IARSIENNILNDYQFVEAGIAFNCDIEHSGYNKITNILVPSENTKENYFSLKFSMNKE
                     "
BASE COUNT         2044 a         1399 c          988 g         2587 t
ORIGIN      
        1 tttttgatat taccgaagtt gattatgcta ttcttgagac aagcggcaaa ttaacagtat
       61 taaaaaagca aaaattcgac cctataacga aaaaagatat gcccataccg gttaagcaaa
      121 atagaaactt gcctgtgcaa ataattaata acggtaaaat tatcgaaagt gttatgacag
      181 aatttaatct tgatattgac tgggtatata gcgaagcaaa aagactggga tataaaaata
      241 taaaagatat tttttatgca ggtatcaatt gcgacgggca atttttcatt gttccgctct
      301 ttaatgaaaa aaagtataaa caataaatca atggcctaga tatccatata ttttaccaca
      361 gtatcggaca aagaaattat ttctgagcaa ataatatcct tgtaatccat aagggtcttc
      421 tcatccatat tgataaaact atcatccgga gaagttaagg cagctttaga aaatatttca
      481 atatcttttt tattaggagc tttaaaaact tcttttttaa gtattattcc ctttgaaata
      541 taaaacaact tgtagccttc atcaaacgga attgatacaa ttacgttact tttaagaagt
      601 tcattgtatt tattgattga attaataaga tatcttacat taattaagat gtctttgtag
      661 aaagaagcct tttcaaaatc acataataaa gcttctcttt tcattttatt ttctaactct
      721 ttaataaata tgcctgcctt tgaattatct gttaatattt catataaaga atatctgttt
      781 ttgttaaatt caatctcatt tagtgttaga ggaaacaggt gaaattcaaa ggaatatgat
      841 tcgccctttt taattatggg gtatatattt tttaaactgt tgatagtttc atttaaatga
      901 aatttttttc taaaaggtcc aaaacaattt tcttctctgg attcaacaac tgataaggct
      961 ctgtttttat tataattctc aagctttaaa taaacataac ccctgtcatg cttcatttgt
     1021 aaattaaaaa taggttttaa ttcttttatt aaactgcatt ccaaaatacg agcctctaga
     1081 tgagtatccg ttacaataaa atcaatatca tctattaaac gaaccattct gttaatcttg
     1141 tcccaattag gattgtttgc aaagtatgtt tttactcttt tattgagcat tttgcttttt
     1201 cctatataaa taacgttacc ctttgcatca atcattttgt atattcccgg ctccatagga
     1261 agcatatcta attttttctt tattttgtca gaaatattta tcaataaatc acctgtttct
     1321 tcctggttag tgttctttac tattaaagct ttacatacag tttcattata ttagttaaag
     1381 acttttcttc tccttgtttc aaacggcact ctgctcttaa aatataatat tatctgttca
     1441 ctgcatgcat tttaatttta cacctttaag tacttttccc aatatgttaa agcttatagg
     1501 tttagccaaa tgtatgttaa atcccgtttc ccttgaacgc tcaacatctt caggctgagc
     1561 atagccggaa gaagcaataa gatatgtatc tttcaattca gagtcctttc ggattatttt
     1621 tgcaacctcg tatccgctca tatccggaag acctatgtca catattatta catccggtct
     1681 attttccctt gctttagcaa taccttcagt tccgttctta gccgataatg ctctgtaacc
     1741 tgataaaaca agcatctcac acattatatc aaccaaatct ttattatctt caattattaa
     1801 aatatttagt atatcatttg acatagcaaa tatcctccga tatttattta aataggtagc
     1861 cttattgtga atttagaacc ttttcctacc ccttcgctaa atacttctac tctgccgcca
     1921 tgaagttcca ctactctttt gacaatagtc aaaccaagtc caagtcctcc aagttttcga
     1981 tccaatgatt tgtccacctg catgaatggt tcaaacagat ttggctgatc tgtctggtct
     2041 ataccccgtc cggtatcttc aacagcgatt acagcttcac tgctattggt gtccagtgct
     2101 gcagaaacaa ttaccaaatc cttttcattt gtgaacttta cagcgttgtg cagcaggttg
     2161 ccaattgctt gagctaatcg tgaagaatct gcttctatat acactggtat ggtcgattta
     2221 aattccaact ttacattttt atcaataaat tgaggctggt aatcttgaac tactttgcct
     2281 ataagattgt tgatttctaa agtatctttt tttaaataaa ccttattgtg tgtaatgcgg
     2341 gttacatcta atagatcgtc aaccaaatca gtaaggagtt ttgcttgacg tttgacgata
     2401 tccatcgcca tataagcttg tttcccgtca gaaggtgcct tttccaagag ctccagcccc
     2461 ataattattg atgcaagcgg atttctcaat tcgtgagaaa gcatattgat aaaaacattc
     2521 ttattatggt cagcctgacg aagtttctcc actaaatcta atgcattttt ttcactttcc
     2581 gcaagagctc tttcagcttg tcttagcgat gttatgtcca ttgcaacagc cacataattg
     2641 ataatattcc cgtccttatc acggagagct tcggttgaag caacctgagt gcctctcgta
     2701 ccgtcaaacc gttcaaaatc caacatcaca ccaaccagcg cttctttaag tggatatggt
     2761 tcaaaagtta ccggttcgtc ggtatcgggc agaaataatc tatatgacgt gtattcttta
     2821 ggaatatctg tcattgaaga actgccccga tacatattta tcgcttcttg atttaccata
     2881 atcacttttc cgctataatc cgaaatccaa acgcctgcag gcattaaatc cattatggtt
     2941 tgaagccaag cagctctttc ttctgcaatt ttcatataat gacttttttc tatctcgatt
     3001 tttttgcggt cgttaagggc ttttttttct gatgcttctt tatttttcaa ttctgttata
     3061 tcctgaaata aaatcgcaaa cttgttctga cttccaagcg gaatcacttg aacatcaaac
     3121 catataccaa gtgcttcaat gtatatttct aacctatcaa atttacccgt ttttacggct
     3181 tcaccatatc gtataaacca ttctttttca ataaaagaat agatttcttt aattcttttt
     3241 cctacaattt cttcccgttt taaccccata tacttttcag cggtacgatt tacgtctaaa
     3301 agcaaataat caaccggttc ccccgtttca ttaaagacta tctggttgat ttgaattgct
     3361 tgcggtaaat tttcaaataa tattcgataa aagtctgttt catgaataaa ggtattaact
     3421 atgctcattt aaatacctcc taacatggtt ctcggattat aggttaattg caataaaata
     3481 attatgttat ttcatatatt atacactaag aagattggga attttgtctt attttagtaa
     3541 tttttgttat atttttatga attttcttta atatttctta ataaataacc agttaagaac
     3601 caataaacag ttaatacaat atagactttc accatatgac ttcagctttt gattcctcaa
     3661 tatgacagta caacaattat tcaatttcat atattttaga gagttcaaca gctatttttg
     3721 ccccaagtga ggcattgttg tatactaaca taatatttga ttcaaggctg tcgccgccgg
     3781 ttatctcttt tacttttgcc agcaaaaacg gagtagtatc ttttcccttt ataccgtttt
     3841 catcagcttg tttaacagcc ttattaattg catccgtaat tatatcataa tccatctgat
     3901 attgttcttc tatagggttt gctactacca tacccccttt aagtccaagg tcccattttg
     3961 cttttagcat ttttgcaatt tcttcaggag tatcaagctt ataatcaact ttgtagccgc
     4021 tttttctggt ataaaatgcc ggcatttctt ctgtttgata tcctactacg ggaactccat
     4081 gagtttctaa gtattccaaa gttaaaccta tatccaaaat ggattttgct cccgcgcata
     4141 caaccgccac atttgtattt gctaattctt caaggtctgc agaaatgtca aatgtttcag
     4201 ttgctcctct atgaacaccg ccgatacccc ccgttgcaaa cactttaatt cctgcaagag
     4261 aagctacaat cattgtcgaa gcgacagttg tagccccgtc taattttttg gaaacgatgt
     4321 atggaatgtc tcttcttgat gctttaatta catttgttgc ttttcccaag tattctattt
     4381 ctttttcagt gagtccgacc ttcagtttac cgcttaaaat tgctattgtg gcaggcacgg
     4441 ccccgttatc tcgtataatc ttttctacat taagtgctgt ttcaacattc ttaggatatg
     4501 gcataccgtg tgatataatt gttgattcta atgctacaac aggttttccc tgttctaatg
     4561 ctgtttttac tttcggatta atatctaaat attttttcat cataattcaa ctccttcatc
     4621 cctccaacat tccatctatt ccaccggttt ctagaattta ttcttggtaa tcatcaactg
     4681 cagtgttatc tatgcccgat gttttgataa gaacggctct tccttcatca ttccatttta
     4741 catctgcatt taagctttct gcaatgaatc taacaggtac taatgttctt gaatttttaa
     4801 tttttgcagg aacatctaac tccaccggct cattgttaat atatgctgta ttatcgtcaa
     4861 tttttaaaga tatggttgta ttatctttcc tagcaatagc tgttttattt gtatcatccc
     4921 actcaacttc cgctccaaga gcttcaaata ttgctctcat cggcacaagg gttcttgagt
     4981 tttctataat tggatctgta tcctcaaaat ttagaagctt gtcatctata aatactctga
     5041 ttagttttct gtccttataa gcatcaatgt tttccggtaa ttctaccaag cctatagctg
     5101 tacctccatt attgtcagct ataataagag catagccttc attaaaagaa cttaaatatt
     5161 gtatgtttaa cgatgccgga attacttcag caccatattt atttagcatg actatgaatt
     5221 cttttgggct tggaccatag gttgacaatg ctcttatgag accatctctc atgtattctc
     5281 ctgcgtatct accatattgc caaaggggag tcaacctttc accatctcta ttaattagaa
     5341 aacttgagcc gtagccattt ggataatttt gattttctat aacaacatag acagcttctc
     5401 ctataaacgg atttgcttct ttgtatttta caggtaatac atagtctcca tttttattta
     5461 tatacccgta ttttcctgaa ctgtcccagg caactgcaag acctccatag aaactgttgt
     5521 caacatattt gtcggtttca ggcattagca taaaatcaat tataatgttt ccttctttgt
     5581 ctatgtaacc tgccataccg tttttgacca cataggcaac ttcttctcta aatggcttag
     5641 cttcatcata tagcataggt ataacaatat cgccatcttt atttatgtac ccccaaagct
     5701 ctgtctcaat atcttgcaca gcagcaagac cttctgagaa atttcctgca tctttatata
     5761 tttgaggaat tactatttct cccgttttat taataaatcc tttattttct tcataaccgc
     5821 caatagtcgt aaacccttca tgaaacatat ctcccatatt actatgcata ccaagaggta
     5881 ttacttcatt tcctttactg tctatgaaac cttccatttg acctggaggt gccataagtc
     5941 tccccggaac atcaggttca tctcgtggta tgtatttata tacttttgcc ataccatcag
     6001 aaaattcatg tattccattg taatcaccaa cagctattat cttaccggtt gtgtcaaaac
     6061 atatagtttg tcctgataat cttagagtaa agatcccttc actgaatata tccatctgtg
     6121 aataagactg tatatgtgag tctccaatat caagccaagt gatatcaagt cccgggaatt
     6181 caagtcccct gcttgctttt tcttggtctt ccgcaacaat tgactgccct tttacatatt
     6241 cagctccata tacatcttct gtaacaaaag gtgcaactgt cattagcaga gttaaaatta
     6301 gtatgataaa aatcctggtt ttcattgtat ttcctcctat agtattactc ttcaaatatt
     6361 attattcttt attcattgaa aattttaaac tgaaataatt ttctttcgta ttttctgacg
     6421 gcactaatat atttgttatc ttattgtagc ctgaatgctc tatatcgcaa ttaaatgcta
     6481 ttccggcttc aacaaattgg tagtcattta gaatattatt ctctatactt cttgctattc
     6541 gtttcgggat ttttgaaaat agccccattt cattctgtgc ccctgctaaa gcaagggatg
     6601 ccaaaaacat atgcttatca tatgaactaa tcatataatc atggtttttc atttcaatgt
     6661 caaatgatac acctgtcaca tatccgtttt ctatcgtaaa tttatattcc ggcttttcag
     6721 ttaaattgat ataaattaca gtcccgtcag tttccttctc tgcccatttc ccgtcttcat
     6781 ccaagtattc attaccaaaa tcaattttgt acagctttgc ataatagttg taattctctg
     6841 caaattctgc tatatttata tctcctttgt tgggaggaag ttgctgtgca gaggatatca
     6901 taaaaaaagc agcaaatgtt gccaaagttg caccgacata aatcacgcct ctataccatt
     6961 tcgtatcctt aatagtgtaa gatattgatt catcccaagg ctgtgtttca ttcgctat
//
LOCUS       DAOSHP010000089         6950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_9463_length_6950_cov_9.307324, whole genome shotgun sequence.
ACCESSION   DAOSHP010000089 DAOSHP010000000
VERSION     DAOSHP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9463_length_6950_cov_9.307324"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..695)
                     /locus_tag="PLB33_07930"
     CDS             complement(147..695)
                     /locus_tag="PLB33_07930"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOG63491.1"
                     /translation="MKIYTKTGDKGQTSLYDGTRVDKDSLRVESYGTVDELNSYIGFA
                     MKFVNDESINESLKKIQMRLFFVAGELATVEEGKYKYKVKEEDIAALEKIIDDYLPKI
                     SGVDKFIVPGSSEASAALHVARTICRRAERRIISLKRHEEVSDILLKFVNRLSDVLYT
                     YARFLESNLTIMDFEKAYNEKL"
     gene            complement(704..1573)
                     /gene="rsmA"
                     /locus_tag="PLB33_07935"
     CDS             complement(704..1573)
                     /gene="rsmA"
                     /locus_tag="PLB33_07935"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOG63492.1"
                     /translation="MDEIKLYSPKVIKDILDRYGFKFSKSLGQNFLIDGNIIKRIVEI
                     AGLDEKSGVLEIGPGFGTLTQLLCKKANKVIAIEVDKSLTEVHKGTLDYPNLKIIYDD
                     FLKVDVNKLIEEEFKGLDVKIVANLPYYITTPIIMKILEEKYKVSKIVVMVQKEMAQR
                     LNSKPGTKNYGAITLAVQYRADTNIAMIVPNSVFMPKPKVDSAVIEFKILPKPRIDVC
                     DEKMLFKVIKASFAQRRKTILNGLSNSLNFSKDIINESLVSAGMNPGIRGEKLTLEEF
                     GRISDEISKRVKD"
     gene            complement(1566..2114)
                     /gene="rnmV"
                     /locus_tag="PLB33_07940"
     CDS             complement(1566..2114)
                     /gene="rnmV"
                     /locus_tag="PLB33_07940"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HOG63493.1"
                     /translation="MIKEIIVVEGKADVSAVKRAVDAQVISTNGLGINDKIINVIKKA
                     SKNKGIIILTDPDYPGKKIRNILASQIENCKHAFIPRDKANKDGDIGVENASKEAIIE
                     ALKNARAEVAENRQEFSYQDMVRYGLVGNDNASKRRSAIGDELGIGYCSAKQFLKRLN
                     SFGISREELEDAIKKTIEDING"
     gene            complement(2107..2766)
                     /gene="udk"
                     /locus_tag="PLB33_07945"
     CDS             complement(2107..2766)
                     /gene="udk"
                     /locus_tag="PLB33_07945"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOG63494.1"
                     /translation="MKKPIFIGVSGGTGSGKTTIVNRICSEVPEKSVAIMEHDSYYKD
                     QSSMTYEERCQTNYDHPFAFDTDLFVEHIKELKKGNSIQKPIYDYSIHNRKKETIIVE
                     PKEIIIVEGLLVFYEERIRELLDIKIYVDTDADIRILRRIIRDMNERSRSLDSVITQY
                     LNTVRPAHEQFIEPSKKYADIIVTEGGNNLVAVDLMVTKIKSLLNMSNSDTITHGANI
                     D"
     gene            complement(3103..3744)
                     /locus_tag="PLB33_07950"
     CDS             complement(3103..3744)
                     /locus_tag="PLB33_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOG63495.1"
                     /translation="MKIKIIYPILILTLAMSFIITYGISEGKFETYAFAEIFEKSEEA
                     EESEAYDEIKDINEITARNMEAKLTLKELKYHIERDSLEQETSLEYKNDQYKKETTSR
                     GGFRFNNAMDMVATAYDLSYESCGKYPDDPEYGITASGTKAQPGTVAVDPDVIPLGTK
                     LYIASTDGSPDYGFATALDTGSAIKGYRIDLFMENNQDAMDFGRRQVKVYILD"
     gene            complement(4091..4960)
                     /locus_tag="PLB33_07955"
     CDS             complement(4091..4960)
                     /locus_tag="PLB33_07955"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOG63496.1"
                     /translation="MKEIPKIMAVQDMSGVGRCSLTVIIPIMSALGCQVCPLPTALLS
                     NHSEYKEFYFFDFTDHMEEYFSYWEKNDFWFDCVYSGFIGSEKQINIIVDIIEKVKNK
                     NNKALIVIDPVMGDCGCTYATYNSEMITKMSQLVKKADIITPNLTEACILLGRKYYSD
                     KITLPQTKECLKDLCSLGPKISLITGIMTSEEEHINVCYDSEQDKYWLAPFSYIDKRY
                     PGTGDLFTSLFLGYYMKGKSLPEAMEEAARFVSLSVRISSEAEAPSSEGVIFEKIMKE
                     LYNEISEYKYTSI"
     gene            complement(5387..6877)
                     /locus_tag="PLB33_07960"
     CDS             complement(5387..6877)
                     /locus_tag="PLB33_07960"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOG63497.1"
                     /translation="MAFIINEPSRTFGEYLLLPNLTTKDCIVENVDLSTPLTRFKRGE
                     APEIKLNIPFSSAIMQSVSDNNMAVALAKNGGISFIYCSQPIESEAEMVRKVKKYKAG
                     FVISDSNLTPEHTLADVLNLKAITGHSTMAITEDGSPTGKLLGIVTGRDYRTSRDSLD
                     KKVKDFMTSREDLIVGREGITLSEANDIIWEHKLNSLPIVDKDFNLVYMVFRKDYSED
                     KENPNSLLDAKKRYVVGAGINSRDYKERVPALVEAGADILCIDSSEGFSEWQHETINW
                     IKQRYDGKVKVGAGNVVERDGFKYLADAGADFVKVGIGGGSICITREQKGIGRGQASA
                     VMDVAAARDEYFEKTGEYVPICSDGGIVLDYHITLALAMGADFVMLGRYFAGFDESPT
                     NKIKYGSGYVKEYWGEGSNRARNWARYDLGGNAGLHFEEGVDSYVPYAGKLDDGLKTT
                     LHKIKSTMCNVGTKTIKELQQNARLTVVSATSLTEGGAHDVILKNQ"
BASE COUNT         2001 a         1468 c          969 g         2512 t
ORIGIN      
        1 tgagcgaagc gaaggatctc atgagattct tcgagctaaa gctctcagaa tgacattcca
       61 tgggctacta atatcaatga caccctgcaa tatatgaata cgctttgtca tcctgagcga
      121 agcgaaggat ctcataatag tgtattctaa agtttttcat tataagcctt ttcaaaatcc
      181 attatggtca aattactttc taaaaatctc gcatatgtgt ataatacatc cgacagcctg
      241 tttacaaact tcaaaagtat gtcacttact tcttcgtggc gttttaaaga tataattctc
      301 ctttcagccc tgcggcaaat cgttcttgca acatgaagtg cagctgaagc ttccgaagag
      361 ccgggtacta taaatttatc gactccgctt attttaggaa gataatcatc tattattttt
      421 tccaatgcgg ctatatcttc ttctttaacc ttatacttgt atttcccttc ttcaacggta
      481 gccaattctc ccgccacaaa aaataagcgc atttgaattt tcttaaggct ctcgtttata
      541 ctttcatcat taacaaattt catagcaaat ccgatataag aatttaattc gtcaaccgtt
      601 ccgtatgatt ccactcttaa tgaatcctta tctactcttg tgccgtcata taaagatgtt
      661 tgacctttat caccggtctt ggtataaatt ttcataattc ctcctaatct tttactcttt
      721 tacttatttc atccgatatt cttccgaatt cctccagtgt tagtttttct cctcttatac
      781 ccggattcat gcctgcacta accagagatt catttattat gtctttagaa aaatttaaac
      841 tattgcttaa tccgttaagt attgtctttc gtctttgagc aaatgaagct ttaatcactt
      901 taaaaagcat cttttcatca catacatcga ttctcggttt cggtaaaatt ttaaactcaa
      961 taacagcaga atcaactttt ggttttggca taaataccga atttggaact atcattgcaa
     1021 tatttgtgtc tgccctgtac tgaacggcaa gagtaatagc accataattc ttggttccgg
     1081 gttttgaatt aagcctttga gccatttcct tttgtaccat tacaacaatt tttgaaactt
     1141 tatatttttc ctccaatatt ttcataatga tgggtgtagt aatgtaataa ggtaaatttg
     1201 caactatttt tacatcaagc cccttaaatt cttcttcaat taatttattt acatcaacct
     1261 tcaaaaagtc gtcataaata atttttaagt tggggtaatc aagtgtaccc ttatgaactt
     1321 ctgttaagct tttatctacc tctatggcaa ttaccttgtt tgctttcttg cacaaaagct
     1381 gggtcaaggt gccaaagccc ggtcctattt ccaatactcc cgacttttca tccaaaccgg
     1441 ctatttctac aattcttttt attatattcc cgtcaataag gaaattctgt cccagactct
     1501 tgctgaattt aaatccgtac ctgtcaagta tgtccttaat aacttttggt gaatacagct
     1561 ttatttcatc cattaatatc ctcgattgtt tttttgattg catcttctaa ctcttccctg
     1621 cttataccga aggaattaag cctctttaaa aattgttttg cgctgcaata tcctatacct
     1681 aattcatcac ctatggcact tcttctttta gatgcattat cattcccaac caatccatag
     1741 cgcaccatgt cttggtaaga aaactcctgt ctgttttctg cgacttcagc ccttgcattt
     1801 ttcagcgctt caattattgc ttcttttgaa gcattttcaa ctcctatatc cccgtcttta
     1861 tttgccttat ccctgggtat gaatgcatgc ttgcagttct ctatttggga agcaagaata
     1921 tttcttattt tctttcccgg atagtcaggg tcggtcagta taataattcc cttgtttttc
     1981 gatgcctttt taataacatt aataatttta tcatttatac ctaatccatt ggttgaaatc
     2041 acctgtgcat caacagccct cttgactgcc gaaacatctg ccttgccttc aaccacaata
     2101 atttccttaa tcaatattcg ctccatgtgt aatagtatcc gaattgctca tatttaacaa
     2161 agactttatt tttgtaacca tcaaatcaac tgccactaaa ttattgcctc cttcggttac
     2221 aataatatca gcatattttt tacttggctc tataaactgt tcatgagcag gccttaccgt
     2281 atttaaatac tgggtaataa cggaatcaag acttctgctc ctttcattca tatctcttat
     2341 aattcttcta agaattctta tatctgcatc tgtatcgaca taaattttaa tatcgagaag
     2401 ttctcttatt ctttcttcat aaaataccaa aagtccctcc acaattatta tttcctttgg
     2461 ctcaacaata attgtttcct ttttcctgtt atgtatgctg taatcataaa taggtttttg
     2521 aattgaattt ccttttttca attccttaat atgctcaaca aacaaatccg tatcaaatgc
     2581 aaatggatgg tcgtaatttg tttgacatct ttcttcataa gtcatcgaac tttggtcttt
     2641 ataataagaa tcatgctcca taattgctac gcttttttcg ggaacttctg agcaaattcg
     2701 atttacaatt gttgttttac cggaaccggt tccacccgat acccctataa aaattggttt
     2761 cttcataatt tcctctcaaa tgtttattat aataataata tacaacattt tttcgaattt
     2821 taccacatac aaataaatca taacaaaaaa ataatgttca atcattaaat aatatgctaa
     2881 gtgtcattct gagagcttta gctcgaagaa tctcatcttt ataaaacctg agatccttcg
     2941 catacgctca ggatgacaat caaagagcat atttgtttat cgtggcttgg taaaaacatg
     3001 atatttctga atctcttttt atacgtgaaa aaattgctgt ctccagcaat ttttcttcgg
     3061 ggacatttct cgcatgtccc cataactttt aacttaatga tattagtcta atatatagac
     3121 ttttacctgt ctcctgccaa aatccattgc atcttggttg ttttccataa acaagtctat
     3181 tctgtaccct tttatcgctg agcctgtatc aagggctgtt gcgaaaccat agtcaggact
     3241 gccatcagta gaagcgatat acagcttcgt acctaaaggt attacatcag ggtctaccgc
     3301 aaccgtaccc ggctgagctt ttgtgccgga tgctgttatt ccgtattcag ggtcgtccgg
     3361 atatttccca cagctttcat atgataaatc ataagctgta gctaccatat ccattgcatt
     3421 attaaatctg aaaccaccac gagaggttgt ttctttttta tattgatcgt ttttatattc
     3481 taaagatgtc tcttgttcta aactgtctct ctcaatatga tactttaatt cttttaatgt
     3541 aagttttgct tccatattcc ttgcagttat ttcattaatg tctttaattt catcatatgc
     3601 ttctgattct tcagcctctt ccgacttttc aaaaatctct gcaaaagcat aggtttcaaa
     3661 cttaccttca ctgataccat aagttataat aaagctcatt gccaatgtta aaatcaaaat
     3721 agggtatatt attttaattt tcatattacc accaacctta tatttgaatt gactaagacg
     3781 tctattaagg tataaatttc ttccggaaat ttatgttttg cataagtatg agcttatgca
     3841 ttattggcag tcccttttta aaaaactcat aatttttggc ataaggagaa ttatacttta
     3901 aaaaaaaagc ccggtcaagt ctttttttga aaaatctgcc ttctaatgcc tatattgttg
     3961 caattgccta ctttttatcc aatcctttat tttaactttt ctgctataat aaggcagaat
     4021 ttttagaaca ttttccaaaa atggcataca aaaacagcct gtaaaaggct gtttttcact
     4081 gcaatatttt tcatatactc gtatatttat actccgaaat ttcattgtac aattctttca
     4141 taatcttttc aaaaattact ccttctgagc ttggtgcttc agcctcggaa cttatacgaa
     4201 ccgaaagcga aacaaatctt gctgcttctt ccattgcttc aggaaggctc ttccctttca
     4261 tataataacc caagaataag gaggtaaata aatcccccgt acccggatat cttttatcta
     4321 tatagctgaa aggtgccagc caatacttgt cctgctcgct gtcgtaacaa acattgatat
     4381 gttcctcctc agatgtcata attccggtta ttaatgatat tttaggtccc agactgcata
     4441 aatcctttag gcattccttt gtttgaggaa gagtaatttt gtcgctgtaa tattttcttc
     4501 caagcaatat acaagcttct gtcaaattcg gtgttataat atcagctttt tttaccaatt
     4561 gtgacatttt tgtaatcatt tcactgttat atgtggcata agtgcaaccg cagtcaccca
     4621 ttacagggtc tatcacaatc aatgctttat tatttttatt cttaaccttt tcaataatat
     4681 cgacaataat gtttatttgt ttttcagagc ctataaaccc gctgtaaaca caatcaaacc
     4741 aaaaatcatt cttttcccag tatgaaaaat actcttccat atggtctgtg aaatcaaaaa
     4801 aataaaattc cttgtattcg gagtgattgc ttaataaagc tgttggtaag gggcacacct
     4861 gacatcccaa agcagacata atcgggataa tcacagtcaa tgagcatctc cctactcccg
     4921 acatatcttg aacagccata attttaggaa tttctttcat aatttatcct ttcattatag
     4981 ggattttacc tgatacgggg acattccttc agtccccata catattttaa cttattcttg
     5041 tactttcaaa aggtacagat ttatcaaatt ttaagaggac agttttcata tcatacgcat
     5101 tgaatgcatc aaatttacta aaaaaaatga tttctatata aatagaaatc attttttaca
     5161 gggtaaagat ttattaaata actctttaca aggggggcta atttatttca taatatatat
     5221 tcgagcttgt acaatgtttt tttggtgtta ttctcaaaaa atattttacc agttttttac
     5281 accatattct acaaaattct ctacatatag tgtagcaaat ccattgcaca tttgcaagaa
     5341 aaattatttg aaaattagaa atttctgtaa agttttacta atctaactat tgattcttca
     5401 aaattacatc atgagctccg ccttcggtaa gacttgttgc agaaaccaca gtaagccttg
     5461 cgttttgctg aagttccttt attgtttttg taccaacgtt gcacatggtc gattttattt
     5521 tgtgaagtgt tgttttaaga ccgtcatcaa gcttgcctgc ataaggaaca tatgaatcaa
     5581 cgccttcttc aaaatgaagt cccgcatttc cgcctaaatc atatcttgcc cagtttcttg
     5641 ccctgttgga gccttctccc caatattcct ttacgtatcc gctcccgtat tttattttat
     5701 ttgtagggct ttcatcaaat cctgcaaaat accttccaag catcacgaaa tcagctccca
     5761 ttgccaaagc aagggttata tgataatcca ggacaattcc tccgtcagag catattggaa
     5821 catattctcc ggttttttca aaatattcat cacgggcagc agcaacatcc attacagccg
     5881 aagcctggcc tcttcctatt cccttctgct cacgcgttat gcatattgag cctccgccta
     5941 tacctacctt aacaaagtca gcacctgcat cggccaaata tttaaaacca tccctctcaa
     6001 ctacattgcc ggcacctacc ttaaccttgc cgtcatatct ttgctttatc caattaattg
     6061 tttcatgctg ccattcacta aaaccttcgg atgagtctat acaaagaata tccgctccgg
     6121 cttcaactag ggcaggaacc ctttccttgt aatctcttga attaatacct gctcccacaa
     6181 catatctttt cttagcatcc aacaaggaat tagggttttc cttgtcttcg gaataatcct
     6241 ttctaaatac catgtatacc aaattaaaat ctttgtctac gatgggaaga gaattcaact
     6301 tgtgctccca aattatatca ttggcttccg aaagagtaat accttctctt cctacgatta
     6361 gatcttctct gcttgtcata aagtccttaa cctttttatc cagggaatcg cgagatgtcc
     6421 tgtaatccct gccggttaca attccaagaa gctttcccgt tggagagccg tcttctgtta
     6481 tggccattgt cgaatgaccg gttatagctt tcaaatttaa aacatccgct aaggtatgct
     6541 ccggtgttaa gttggaatcg cttatcacga atccggcttt atatttttta actttcctta
     6601 ccatttctgc ttcactttct atcggctgtg agcaatatat aaaagatata cctccgtttt
     6661 tcgccaaagc tactgccata ttgttatcgg aaacagattg catgatagct gaagaaaaag
     6721 gtatattaag ctttatttca ggagcttcgc ctcttttaaa ccgagttaac ggtgtactta
     6781 aatcaacatt ctcaacaata cagtcttttg tggtcaaatt aggtaaaagt aaatattctc
     6841 caaaagttct tgaaggctcg tttataataa atgccatctt aatctccttt tcttattgtt
     6901 aatcgttttt taataattgc tatcatacca tattactaat gtcaactcaa
//
LOCUS       DAOSHP010000090         6387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_10669_length_6387_cov_9.355180, whole genome shotgun sequence.
ACCESSION   DAOSHP010000090 DAOSHP010000000
VERSION     DAOSHP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10669_length_6387_cov_9.355180"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1525
                     /locus_tag="PLB33_07965"
     CDS             143..1525
                     /locus_tag="PLB33_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOG63498.1"
                     /translation="MNENKELFESFPIPKALAALAIPTIISQLITMIYNMTDTFFIGR
                     TNDPYKVAAASLAYVLFFIITALSNLFGIGGGSLISRLLGGQQEHEARKVCSFSFYGT
                     IIVTLIYSACCFLFMNPLLRIMGASDNTIGYASSYTLWVVVIGGVPSTLNMTMAHLLR
                     SEGYAKQASFGVGMGGLLNIALDPIFMFILLEPGYEVTGAAIATMLSNVCALIYFFIV
                     FAYLRKKTVLSLSPRLILPGTKYIREILLVGLPSALGTFLACVSNTTINRLASGYGDI
                     PVAAIGIVKKIDMLPMNVGKGLCQGMMPLVAYNYASKDYKRMHSAAKCSRICGMTFAG
                     ICIVTYEIFAESIVGLFIKDAQTLNMGINFLRICCIATPLMICNVQMSYTFQAMGKGP
                     QSLLLSSCRQGLVNIPLLFLMNALFGLYGVVWTQLLADGITLIISFTLYQKLYNTLKS
                     HYGELISVSN"
     gene            complement(1565..2905)
                     /locus_tag="PLB33_07970"
     CDS             complement(1565..2905)
                     /locus_tag="PLB33_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOG63499.1"
                     /translation="MQNSLEQITIKLGGLTCTACEYKIEKALKKLDGVDSVKVSFASS
                     MAEIIYNSDKRDLNTIYDTIEKTGYEVVYDYDEIEEDSSNTSTFLILLIIAGLYIIIK
                     NTVGFNFLPQITQSMGYGMLFIAGLFTSIHCIAMCGGISLSQCAGCGDCENKISSKLK
                     PGFLYNLGRVISYTILGGIVGALGSVFSLSLKGKSIISLIAGAFMIVMGINMLNITPA
                     LRKLMPRLPKNLTEKIDSKKNNKGAFIVGLLNGFMPCGPLQAMQLYALGTGSFLTGAL
                     SMFIFSLGTVPLMFAFGAFSSLLSLKFTRQMMRITSVLVIILGVVMGNRGLLLSGINV
                     SEAILSPMGISLSKDNKNADAVIKDGYQTVTTNLEPGRYQPINVYAGVPLKWTIVVEE
                     GKLNGCNNELIIPAYGKQQKLEIGENVIEFTPTQAGTITYTCWMGMISSTITIK"
     gene            complement(2914..3441)
                     /locus_tag="PLB33_07975"
     CDS             complement(2914..3441)
                     /locus_tag="PLB33_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HOG63500.1"
                     /translation="MKANKTKKVKKNNNLVLGVVAVIIIGIAAFTFLNSGKDDEIAKN
                     SKPTAISATDENGDMVIQTSDITEIASFYGYDELDTYIEVLAVKASDGTIRTAFNTCQ
                     VCYSSGRGYYVQQGDYLICQNCGNRFKLDDVEVTRGGCNPVPILDDMKEVTDTTITIP
                     KDLFAEAEVIFANWR"
     gene            3622..5643
                     /gene="fusA"
                     /locus_tag="PLB33_07980"
     CDS             3622..5643
                     /gene="fusA"
                     /locus_tag="PLB33_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOG63501.1"
                     /translation="MKTYNADKIRNVALLGHSSCGKTTITEALLLNTKVTNRMGKVED
                     GNTVSDFDKEEKERQVSIGTSVIPIEYKDTKINFLDTPGYFDFVGEVYGALRVADSAV
                     LVIDASSGIEVGTEKAWKYLEQRNMPRIIFINKLDKENIKFDELLDNLRDTFGKKVIP
                     FTLPIGKGENIDGYMDILKGQSYIDSKPVETFPDTAAKAEELKAILMESVAETDEELL
                     EKYFEGEEFTEEEIQTGLKKAVLEGELVPVIVGSAIKGLGIDMLMNTVVEYLPNALDA
                     KSLKGSPSDSLSAFIFKTIVDPFVGKISLYKVMSGKMKKDTDVYNANTGDTERIGSVF
                     SLRGKEQLEVSEVEAGDIGATSKLQYFKTGDTISLKTAPVSYDKIDFPKPCYFMAVKG
                     KTKDSDEKIGTGLQRLNEEDPTFSIERNIETKELIVGGQGNIQLDVLASKLKNNYKVD
                     VELSPPKVAYRETIKGKSDVQGKHKKQSGGAGQYGDVRIRFEPSQEEFEFTEEIFGGS
                     VPKNYIPAVEKGLRESLEKGVLAGCKVVNVKATLYDGSYHEVDSNEMAFKIAASLAFK
                     KGMLEAKPILLEPIAKVEINIPDEYLGDIMGDMNKRRGRILGMEQQADGSQLVKAEAP
                     MAEMYTYAIELKSMTQARGTFEVEFLRYEEMPNNLAEKVIAEYKAANEN"
     gene            complement(5809..>6387)
                     /locus_tag="PLB33_07985"
     CDS             complement(5809..>6387)
                     /locus_tag="PLB33_07985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOG63502.1"
                     /translation="YEKHMSLDSVFQLQAMNQMMKEQFYTYPIKSIMIFGIAGGNGLE
                     HINNQVINRVYGIDINEKYLNTCKKRYPELEGILETLCKDLTQDIELPYADLLVANLL
                     IEYIGYEYFQKAVKKVNPKYVSCIIQVNTNDSFVSDSPYLHVFDRLDEILHPVEESGL
                     VEAMRNINYKKDRLQERKLPNGKKFIRIDFTR"
BASE COUNT         2077 a         1116 c         1173 g         2021 t
ORIGIN      
        1 aaggttctat acgttgcagt caacatttag attattggct gctttttctt attgaaatat
       61 tgacgtaaaa tgaaagtagt gatacgatat atcgtatgcg ataacatgaa ttccgccaga
      121 cagaaagagg taagtaacaa ccatgaatga gaataaggaa ctttttgaat cattccctat
      181 acctaaagcg ttagcagcct tggcaattcc tacaattatt agccaattga ttacaatgat
      241 ctacaacatg acagatacat ttttcattgg aagaaccaac gatccttata aggttgcagc
      301 tgcatccctt gcttatgtgc ttttcttcat tattactgcg ctttctaatt tgtttggtat
      361 tggcggcggc agtttaatat cccgattgct gggcggtcag caggagcacg aagcaaggaa
      421 agtatgctcc ttcagctttt atgggactat cattgttaca ctgatttatt ctgcctgctg
      481 ctttctgttt atgaatccgc ttttgcgtat tatgggagca agtgataata cgatcggata
      541 tgcttcttct tatacattgt gggtcgttgt aatcggtggt gttccgtcta ctctcaacat
      601 gacaatggct catctgctcc gtagtgaagg ttatgcaaag caagcaagtt ttggtgttgg
      661 tatgggcgga cttttgaata ttgcactgga tccgatcttt atgtttattc tccttgaacc
      721 gggatacgaa gtaaccggtg ctgccatagc aacaatgctt tccaatgtat gtgcgttgat
      781 ttactttttt atcgttttcg cctatttgag aaagaagaca gttttatccc tatctccccg
      841 cttaatctta cccggaacaa aatatatcag ggaaatctta ttggttggat taccttctgc
      901 attgggtaca tttctcgctt gcgtttccaa tacaacaata aacagactgg cttccggata
      961 cggcgatatt ccggtagcag caataggaat cgtaaagaag atcgatatgc ttccaatgaa
     1021 tgtgggaaag ggcttatgcc agggaatgat gccgctggtt gcctataatt acgcatcaaa
     1081 agattacaaa cgaatgcatt ctgccgcaaa gtgctccaga atctgcggca tgacttttgc
     1141 cggaatctgt attgttacat atgaaatatt tgccgaaagc atcgttggtt tatttatcaa
     1201 agatgcgcag actctaaata tgggaatcaa cttcctgcga atttgttgta ttgctacacc
     1261 tttaatgatt tgcaatgttc aaatgagcta tacatttcag gcgatgggta aaggaccgca
     1321 gtcattgctt ctgtcttcat gccgtcaggg actagttaat attcctttac tgtttctgat
     1381 gaacgctctg tttggattgt atggtgttgt ctggacacag ttgttggcag atggtatcac
     1441 tctgataatt tcttttactt tgtatcagaa gctatacaat acactaaaaa gtcactatgg
     1501 tgaattaatt agtgtttcaa attagagtga gaaggtctta aatggaacta cccttcatga
     1561 gcttttattt tattgtaatt gtgctgctta tcattcccat ccaacaggta taggtaatag
     1621 tacctgcttg tgtaggagta aattctatta cattttcacc tatttcaagt ttttgctgct
     1681 tcccatatgc cggaataatt aattcgttgt tacagccatt tagttttcct tcttcaacaa
     1741 ctatagtcca tttcaaagga acacctgcat agacgttaat aggttgatat cttccgggtt
     1801 caagatttgt tgttacagtc tgataaccat cttttataac agcatcagcg ttcttattgt
     1861 ctttagataa acttatcccc ataggtgaca atattgcttc agatacatta atacctgaca
     1921 gcaataagcc tctgtttccc ataactacac ccagtattat tactaaaacc gatgtgattc
     1981 tcatcatctg tctagtaaat ttcaagctca gtaatgagct gaatgcacca aatgcaaaca
     2041 ttaatgggac agtacccaga ctaaaaataa acatggacaa agctcccgtt aaaaagcttc
     2101 ctgtaccaag ggcatatagt tgcatggcct gcaaaggtcc gcaaggcata aatccattaa
     2161 gtaatcctac tataaatgct cccttattat ttttcttgct gtcaattttt tcggttaaat
     2221 tcttaggcag gcggggcatg agttttctta acgcaggcgt aatatttagc atattaatac
     2281 ccatgacgat catgaaagca cctgcaataa gcgaaattat tgatttacct ttaagtgata
     2341 aactaaaaac agaaccaagg gcaccgacta ttcctccaag aattgtatag gatataaccc
     2401 ttccaagatt atataaaaaa cccggcttta gtttacttga tattttgttt tcgcaatcac
     2461 cacatcctgc acattgtgac aaacttatcc caccgcacat tgcaatacag tgtattgacg
     2521 taaacagtcc ggctataaaa agcattccgt aacccatgct ttgagtaatt tgcggtaaaa
     2581 agttaaatcc gacagtattt ttaataatta tatacaatcc ggcaattatt aataaaatta
     2641 aaaaggttga tgtattagat gaatcttctt caatttcatc ataatcatat acaacttcat
     2701 atcctgtttt ttcaatggtg tcataaatag tatttaaatc tcttttatcg ctattgtata
     2761 taatttctgc catggatgaa gcaaagctta ctttaacgga atcaacacca tctaattttt
     2821 ttaatgcctt ttctatttta tactcacaag ccgtgcaggt taatccgcct aattttattg
     2881 ttatttgctc taatgagttt tgcataatat accttatctc caatttgcaa aaataacttc
     2941 agcttcggca aataagtctt taggaattgt tattgttgta tcggttactt ctttcatatc
     3001 atcaagtatg ggaaccgggt tacatccacc ccttgtcact tcaacatcat ccaatttaaa
     3061 cctgttgccg cagttttgac aaatcaggta atctccttgc tgaacataat atcctctgcc
     3121 tgatgaatag caaacctggc aagtattaaa tgcagttctt attgtcccgt ctgatgcttt
     3181 taccgccaat acttcaatat aagtgtcaag ctcatcataa ccatagaatg atgcgatttc
     3241 agttatatct gaagtttgaa taaccatatc tccattttca tcggttgctg atatggcggt
     3301 tggttttgag tttttcgcta tttcatcatc ttttccggaa tttaaaaagg tgaatgctgc
     3361 tataccaata attataacag ccacaacacc taatactaaa ttattgtttt tcttaacttt
     3421 ttttgtttta ttagctttca tatcgggttc ctttccatat taatttgaaa aattataaaa
     3481 aaactttatg aagattttgt gaagatatgt tactctattc tttatgcatc taatatcaac
     3541 aatgtaaaat tgtattgatt atttaataaa tttaatgtaa aatgaaataa ataagcacaa
     3601 cccataggag gaataaagtt aatgaagaca tataacgcag acaaaatcag aaacgtagcc
     3661 ctgcttggcc acagcagctg tggcaaaact acaataacgg aagctctttt gttaaataca
     3721 aaagtaacaa acagaatggg taaagtagaa gacggaaata ctgtatccga ttttgataaa
     3781 gaagaaaagg aacgtcaagt ttcaattgga acatcagtaa taccgattga atacaaagat
     3841 actaaaatta atttcttaga tactcccgga tattttgact tcgttggtga agtttatgga
     3901 gcacttaggg ttgcagacag tgcagtatta gttatcgatg ccagctccgg aatagaagta
     3961 ggaacagaaa aagcatggaa atacttagag caaagaaata tgcctcgaat aatattcatt
     4021 aataaattag acaaagaaaa tatcaagttt gatgaactgc ttgataattt aagagataca
     4081 tttggcaaga aagtaattcc gtttactcta cccataggca agggagaaaa tattgacgga
     4141 tatatggata tattgaaagg acaaagctat atagattcaa aaccggttga aacattcccg
     4201 gacacagcgg caaaggcgga agaattaaaa gcaattctaa tggaatccgt agccgaaaca
     4261 gatgaagaat tacttgaaaa atactttgaa ggcgaagaat ttactgaaga agaaattcaa
     4321 accggattaa aaaaagcagt attagaaggc gaattggttc ctgtaatagt tggttccgca
     4381 ataaaaggat taggcattga catgctgatg aatacagtag tagagtattt gcctaacgcc
     4441 ttggatgcaa aatcacttaa aggaagtcct tcagattcat tatctgcatt tatattcaag
     4501 actatagttg acccctttgt aggaaaaatt tccttatata aggtaatgtc aggaaagatg
     4561 aaaaaagata cggatgttta taatgcaaac accggtgata ctgagagaat aggcagcgta
     4621 ttttccttaa gaggaaaaga gcaattagaa gtttctgaag ttgaagcagg tgatataggt
     4681 gcaacttcca agcttcagta ttttaaaacc ggtgatacta tttcattaaa gactgctccg
     4741 gtatcttatg ataaaattga ttttccgaaa ccttgctact ttatggcagt taaagggaag
     4801 acaaaagaca gtgacgaaaa aatcggcacg ggacttcaaa gacttaacga agaagacccg
     4861 acgttctcaa tagaaagaaa tattgaaact aaagagctta tagtaggcgg acagggaaac
     4921 attcagttgg atgtacttgc cagcaagctt aagaataact acaaggttga tgttgaactc
     4981 agtccaccga aggttgcata cagagaaaca attaaaggca aatcagatgt tcagggaaaa
     5041 cacaaaaaac aatcaggcgg cgcaggccag tatggagatg ttcgtatcag atttgagcca
     5101 tctcaggaag aatttgaatt tactgaagaa attttcggag gatcagtacc gaaaaactat
     5161 atcccggcag tagaaaaggg acttagagaa tcacttgaaa aaggtgtatt ggcaggatgt
     5221 aaagttgtta atgttaaagc aacattgtat gacggctctt accacgaagt tgactcaaac
     5281 gaaatggctt ttaaaattgc agcatcatta gcattcaaaa aaggtatgct ggaagcaaaa
     5341 cctatattgc ttgagcccat tgcaaaggtt gaaatcaata ttcccgatga gtacttggga
     5401 gatataatgg gtgatatgaa caagagaaga ggaagaattc tcggtatgga acagcaagcg
     5461 gacggaagcc agctggtaaa ggcagaagct ccaatggcag aaatgtacac atatgcaatc
     5521 gagcttaagt ccatgactca agcaagaggt acttttgaag tcgaattctt aagatatgaa
     5581 gaaatgccga acaacttagc tgaaaaagtt atagctgaat acaaggcagc gaatgaaaac
     5641 taaagacatg tacgactata ataaagttat taaataataa aaagttgttg taaaaaatca
     5701 cttaaaagat ttattattat cttttaagtg atttgttttt cttactaatt agcttaattt
     5761 gaagatttta taataaatta tgttcataat atttgcaata agccaaattt aacgagtaaa
     5821 atctattctt ataaattttt tcccgtttgg taattttctt tcttgaagcc tatctttttt
     5881 gtagttgata ttacgcatag cttcaactaa cccactttct tccacagggt ggaggatttc
     5941 atctaaacgg tcaaatacat ggagatatgg agaatcggat acaaaagaat catttgtatt
     6001 gacttgaata atacacgaca catattttgg gtttactttc ttaaccgctt tttggaagta
     6061 ttcatatcca atatactcaa ttaagagatt tgccacaagt aaatctgcat atggcaattc
     6121 tatgtcttga gttaaatctt tacaaagtgt ttcaagtatg ccttccagtt ccgggtatcg
     6181 ttttttgcac gtattcaaat atttttcatt aatgtcgatt ccataaactc tattgataac
     6241 ctggttatta atatgttcta acccgtttcc gcccgctatt ccaaaaatca taatggattt
     6301 tatcggatat gtataaaatt gttctttcat catttgattc attgcttgaa gctgaaacac
     6361 actgtctaaa ctcatatgtt tttcata
//
LOCUS       DAOSHP010000091         6376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_10695_length_6376_cov_9.263408, whole genome shotgun sequence.
ACCESSION   DAOSHP010000091 DAOSHP010000000
VERSION     DAOSHP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10695_length_6376_cov_9.263408"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1463
                     /gene="rpoN"
                     /locus_tag="PLB33_07990"
     CDS             66..1463
                     /gene="rpoN"
                     /locus_tag="PLB33_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOG63503.1"
                     /translation="MKMGFELNLSQTQKLIMTPELRQAIQILQFNNVELTDFINKQLE
                     VNPFLEAVDNKSDVSSNEEHMNKNIENEEIDWRELAEKYDDLSYKSYERNIDNENKQS
                     FESYTSKKLSLKDHLMIQLGITVKTNKDKRIGEFIIESLDNKGYLGCSIQDISLLINE
                     DVVDVERVLRLIQTFDPVGVASRNLSECLMIQLKEKGIQDKNAYIIAEKYLEDIATNK
                     IQKIAKELHISVNRVQNICDIIKMLEPKPSRGFIVDSDNIRYIVPDATIEKLNDDYII
                     IINDSYLPTLTINNYYKNMVNELDDKEASRFLADKLNSSMWLIKSIEQRRITLYKVIE
                     SIIKFQRKFFDEGKMALRPLVLKDVADDIGMHESTISRATNGKYVQTPRGLYELKYFF
                     ASSLSEADGSGVSSTSVKSFIRKLIDEENPQKPLSDQKITERLNEQGINISRRTVAKY
                     RDEMRIPSSSMRRRY"
     gene            1627..2634
                     /gene="gap"
                     /locus_tag="PLB33_07995"
     CDS             1627..2634
                     /gene="gap"
                     /locus_tag="PLB33_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOG63504.1"
                     /translation="MVRVAINGFGRIGRLALRLMAQDEDFKVVAINTNSDAETLTYLL
                     KYDTAHGNFMTDKISYKNNLMYVDGKEIITSRVKEPENCNWKELDIDLVIDCTGKFND
                     KEKAEAHIKAGAKKVLLSAPGKGDLKTVVYNVNHNILDGSETVVSAASCTTNCLAPLA
                     KVLNDEFGIVKGLMTTVHAYTNDQNIHDNSHKKGIMARRGRAAAANIVPASTGAAKTV
                     GVVLPELKGRLDGIALRVPTLTGSVVDLTVELGKNVTVEEVNQAVKNAANETIAYTED
                     PIVSSDVIGSRYACVFDALSTQIIEADGKQLVKLIAWYDNEMSFTAQLVRVAKYWGQM
                     R"
     gene            2709..3902
                     /locus_tag="PLB33_08000"
     CDS             2709..3902
                     /locus_tag="PLB33_08000"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOG63505.1"
                     /translation="MNKKTLKDINVKNKKILVRCDFNVPLDKEGNITDDIRIKASLPT
                     INYLLDNGAAVILLSHLGRPDGEANKKYSLQPVAQRLSSLLGRGVCFEDCDVVVDDRV
                     KTNAAKLKPGDIMLLQNTRFRKEETKNAGTFAKELASLGELFVNDAFGTAHRAHSSNV
                     GICEYLHSALGFLVEKEVEIMGNALKTPKRPLTAILGGAKVSDKIAVIENLLNIADNV
                     LIGGGMMYTFLKAKGYNTGNSLLEEDKVELAKNIIEKASEKVVKLILPVDTVVAKEFK
                     NDTEFFTTDVDKIPDEYIGLDIGEKTVEIYKEIIGNSKTIIWNGPLGVFEMDNFANGT
                     NAVAKAIADNEDAISIIGGGDSAAAVEKTGLSENITHISTGGGASLEFLEGKMLPGIE
                     AIDNK"
     gene            3931..4671
                     /gene="tpiA"
                     /locus_tag="PLB33_08005"
     CDS             3931..4671
                     /gene="tpiA"
                     /locus_tag="PLB33_08005"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOG63506.1"
                     /translation="MRKKLIAGNWKMNKDVEESIKLVNILKSSQLNKDVDVLVCPPFT
                     SLYPVIHELKNSSIKVGAQNMHFENSGAFTGEVSPPMLKNIGVEYVIIGHSERRQHFG
                     ETDQMVNKKVKAALNNGLKPILCVGESLEQREAGVEKETVRSQLIKDLDGITEGLNIV
                     VAYEPIWAIGTGRNATSAQANEMSSFIRSVLGDVFTADIADNIVIQYGGSVKASNAKE
                     ILEQSDIDGALVGGASLMADEFIKIINF"
     gene            4685..6220
                     /gene="gpmI"
                     /locus_tag="PLB33_08010"
     CDS             4685..6220
                     /gene="gpmI"
                     /locus_tag="PLB33_08010"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOG63507.1"
                     /translation="MMNKVTALIILDGWGIGPDYEGNAIKRAKTPNFDVLLRRYPNTT
                     LSTSGYDVGLPKGQMGNSEVGHLNIGAGRIVYQDFTRITMSIENGEFEKNEALLSAIN
                     FAKKNNSTLHLIGLLSDGGVHSHNTHLYSLIQLAKKNGLIDVEIHCITDGRDVSPTSS
                     PNYIKELMKKTKQIGVGHIASIMGRYYGMDRNRQWDRVELAYNALINGEGEIYKDPLE
                     AIKSSYLKGVTDEFIKPLLIEKDNGELGTVKDNDAVIFFNFRPDRAREITRAFVDDDF
                     SYFDRKKINVKYVCMTMYDKTIENVEIAYKPNFVQNTLGEYLSRLGIKQLRVAETEKY
                     AHVTYFFNGEIEEPFENEVRILIPSPDVPTYDLKPEMSAFEVKNTVMEQLEKDMYKVM
                     IINFANPDMVGHTGDIEAAIKAVEAVDKCFGEIVNYIIRAEGTAIITSDHGNCEEMFD
                     RETLDIITSHTGNRVPFIVVDNKNFKLREGILADVAPTILELMGLEKPAEMTGKSLIV
                     HNE"
     gene            6232..>6376
                     /gene="eno"
                     /locus_tag="PLB33_08015"
     CDS             6232..>6376
                     /gene="eno"
                     /locus_tag="PLB33_08015"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOG63508.1"
                     /translation="MSIITDIYAREILDSRANPTIEVEVWTESGGYGRAGVPSGASTG
                     AFEA"
BASE COUNT         2405 a          888 c         1331 g         1752 t
ORIGIN      
        1 ttgctttata attatattga aaatttatga atttttgata taatacaatt gtgaggagtg
       61 ataccatgaa aatgggattt gaattaaatc tttcccaaac tcaaaagctt ataatgacac
      121 cggaattaag gcaagccatt caaatcttgc aatttaataa tgtagaatta actgatttta
      181 tcaataagca gttagaagtc aatccttttt tggaagctgt cgataacaaa agtgatgtct
      241 cttcaaatga agaacatatg aataaaaata tagaaaatga agaaatcgac tggagagaat
      301 tagctgaaaa gtatgatgat ttaagttata aatcatatga aagaaatata gataatgaga
      361 ataaacagtc atttgaaagc tatacttcca aaaaactttc actgaaagac cacttgatga
      421 ttcagttagg tatcactgtc aaaacaaaca aagataagcg tataggcgaa ttcattattg
      481 aatcattgga taataaagga tatttgggat gttccattca ggatatcagt ttattaataa
      541 atgaggatgt tgttgatgtt gaaagagttt taaggctcat acaaactttt gaccctgtag
      601 gagttgcatc cagaaactta agtgaatgct tgatgataca gcttaaggaa aaaggaatcc
      661 aggataagaa tgcttatatt attgcagaaa aatatcttga agatattgca acaaacaaaa
      721 ttcaaaagat agctaaagaa ctccatatat cagttaacag ggtgcaaaat atttgcgata
      781 taataaaaat gctggagccc aaaccgtcaa gaggattcat agtagacagt gataatataa
      841 ggtacattgt acctgatgca actatagaaa aactcaacga tgattatata attataatta
      901 atgacagcta cctgcccact cttaccataa acaattatta caaaaacatg gtaaatgaat
      961 tagacgataa agaggcaagc agatttttag ctgacaaatt aaattcatca atgtggctga
     1021 taaaaagtat tgagcaaaga aggattactt tgtacaaagt aattgaatca ataataaaat
     1081 ttcaaaggaa gttttttgat gaaggtaaga tggcattaag gcccttagtt ttgaaagatg
     1141 ttgcagatga tataggcatg catgaatcaa caataagcag agctactaac ggtaaatatg
     1201 tacaaacgcc aagaggtctt tatgaactga aatatttttt cgcaagcagc ttaagtgaag
     1261 ctgatggcag cggagtgtca tccacaagcg ttaaatcttt tataagaaaa ttaatagatg
     1321 aagaaaaccc gcaaaaaccg ttaagcgacc aaaaaataac cgaaagatta aacgaacagg
     1381 gaataaatat atcaagaagg accgtagcaa aatatagaga tgaaatgaga ataccgtcat
     1441 cttccatgag gagaagatat taatagaaaa atatgcaaaa agatatgcat atttttttat
     1501 tataatatgc tacacattat atatttagta tgatataatc tgtttgttac atattatcct
     1561 tattgcaacg gatttattaa taaggattta gaattataat aaatgttatt tctataggag
     1621 gattaaatgg taagagtagc aattaacgga tttggcagga ttggaagatt agcattaagg
     1681 cttatggctc aggatgaaga tttcaaggtt gtagcaatca acacaaattc agatgctgaa
     1741 acattgactt atttattaaa gtatgatact gcgcacggta attttatgac cgataaaatt
     1801 tcctacaaaa ataatttaat gtatgttgat ggcaaagaaa taataacatc aagagtaaaa
     1861 gagcccgaaa actgcaattg gaaagaatta gacattgatt tggtaattga ctgcaccgga
     1921 aaattcaacg acaaggaaaa agcagaagct catattaagg caggagcaaa aaaggtattg
     1981 ctttcagcgc cgggcaaagg cgatttaaaa acagtcgtat acaatgtaaa tcacaatata
     2041 ttagatggaa gtgaaacggt tgtatctgca gcatcatgca caacaaactg tttagctccg
     2101 cttgcaaaag tattaaatga tgaatttgga atagttaaag gacttatgac cactgttcac
     2161 gcttatacaa atgaccaaaa tattcatgat aactcgcata aaaaaggtat aatggcaagg
     2221 agaggacgtg cagcagcagc aaatatcgtt cctgcatcta caggagctgc taagaccgtt
     2281 ggagtagttt tgcctgaatt aaagggcagg cttgacggta tagctcttcg tgttcccact
     2341 cttaccggtt cagttgtaga tttaaccgtt gaacttggaa aaaatgttac tgttgaagaa
     2401 gtaaatcaag ctgttaaaaa tgcagccaat gaaacaatag catatactga agacccgatt
     2461 gtatcaagcg atgttattgg aagcagatat gcatgtgtat ttgatgcatt gtccactcaa
     2521 ataatagaag ctgacggtaa acagctggtt aaattaattg cctggtatga caatgaaatg
     2581 tcatttacag cgcaacttgt gagagtagct aagtattggg gacaaatgag atgatttaac
     2641 caaagacttc gaccgcgtag agatatggtc aacccttaat gacatataaa cgttaacgga
     2701 ggtttatcat gaataaaaaa actttaaagg atataaacgt taaaaataaa aaaatattag
     2761 taagatgtga ttttaatgtt ccccttgaca aagaggggaa cataactgat gatataagaa
     2821 ttaaagcatc tcttccaaca ataaattacc ttttggataa tggtgcggca gtaatattat
     2881 tgtcccattt gggtagacct gatggagaag caaataagaa atattctctg cagcctgttg
     2941 cacaaagatt aagctcactc ttaggaagag gtgtttgttt tgaagactgt gatgtcgtag
     3001 tagatgatag agtaaaaaca aatgcagcaa agcttaaacc tggagacata atgcttttgc
     3061 agaatactag gtttagaaaa gaagaaacga agaatgcagg tacatttgca aaagagctgg
     3121 catcactggg agaactgttc gttaatgatg cattcggcac tgctcacaga gcacattcct
     3181 caaatgtggg catctgtgaa tatcttcatt ctgcacttgg gtttttggta gaaaaagaag
     3241 ttgagataat gggaaatgca ttaaaaactc caaaaagacc tcttactgcc atcttaggcg
     3301 gagcaaaggt ttcggataaa atagcagtaa ttgaaaattt attgaatatt gctgataatg
     3361 ttttaattgg cggaggtatg atgtatactt tccttaaggc aaaaggatat aatacgggaa
     3421 actctctttt ggaagaagat aaagttgaac ttgccaagaa tataattgag aaagcatcag
     3481 aaaaagtagt aaaattaata ttaccggttg atacagtagt agcaaaagaa tttaaaaatg
     3541 atacggaatt cttcacaaca gatgttgata aaattcctga tgaatacata ggacttgata
     3601 tcggtgaaaa gactgttgag atatataaag aaataatagg aaattcaaaa acaataattt
     3661 ggaacggtcc cttaggtgtt tttgaaatgg acaactttgc caacgggacc aatgcagtag
     3721 caaaagcaat tgcagataac gaagatgcaa tatccataat aggcggagga gacagtgcag
     3781 cagctgttga aaagaccgga ctgtcagaaa atataaccca tatttctacg ggaggaggag
     3841 cttcgctgga attcttggaa ggaaaaatgt tacccggcat cgaagcaata gataataaat
     3901 aagaatgccc ctgcatgcgg aggaggtaaa atgagaaaaa aattaattgc aggcaactgg
     3961 aaaatgaata aggatgtgga ggaaagtata aaacttgtta atatattaaa gtcttctcaa
     4021 ttgaataaag atgttgatgt gttggtttgt ccgcccttta catcattata tcctgtaatt
     4081 catgaactta aaaattcttc aataaaagta ggagcacaaa atatgcactt tgaaaacagc
     4141 ggtgctttta caggtgaagt ttcaccgcca atgttaaaaa atattggtgt tgaatacgta
     4201 ataatcgggc actccgaaag aagacaacac tttggcgaaa cagaccaaat ggtaaacaaa
     4261 aaagtcaaag ctgccttaaa taacggtctt aaacctattc tttgtgtcgg ggaatcattg
     4321 gagcaaagag aagccggagt tgaaaaggaa actgttagaa gtcagttaat taaagattta
     4381 gatggtataa cggaaggttt aaatatagtt gtagcatatg agcctatatg ggctataggt
     4441 acaggcagga atgctacaag cgctcaggct aatgaaatgt catcatttat aaggtcagta
     4501 ttaggagatg tttttactgc agatattgct gataatatag tgatacaata tggaggcagt
     4561 gtaaaagctt ccaatgcaaa agaaatattg gagcagtccg atattgacgg agcattggtt
     4621 ggaggagcca gcttaatggc tgatgaattt atcaaaatta ttaattttta agtagaaagg
     4681 ttgaattatg aataaagtaa ctgcattgat tattcttgat ggatggggta ttggacctga
     4741 ttatgagggg aatgctataa aaagagcaaa aactcccaat tttgatgtat tattgagaag
     4801 gtatccaaat acaactttat ctacaagtgg ttatgatgta ggtcttccca aaggacaaat
     4861 gggaaattca gaggtaggac atttaaacat aggcgcaggc agaattgtgt atcaggattt
     4921 caccagaatt actatgtcta ttgagaatgg tgagtttgaa aaaaatgaag cattgcttag
     4981 tgccataaat tttgcaaaga agaataattc aactctgcat ttaatagggc ttttatctga
     5041 tgggggagta catagtcaca atactcattt gtatagttta attcaactcg cgaagaaaaa
     5101 cggcttaatc gatgttgaaa ttcattgcat cactgacggt cgagatgtaa gccctacaag
     5161 cagcccaaat tacataaaag aattgatgaa aaaaacaaaa cagattggcg taggacacat
     5221 tgcctcaatt atgggaagat actacggcat ggatagaaac agacagtggg acagagtaga
     5281 attggcatac aatgctttaa taaatggtga gggtgaaatt tacaaagacc ctcttgaagc
     5341 aataaagagc tcatatttaa agggagttac agacgagttt ataaaacccc tattgattga
     5401 aaaagacaac ggagagcttg gtactgtcaa agataacgat gcggttattt tctttaattt
     5461 cagacctgac agggcaagag aaataaccag ggcatttgtt gatgatgact tttcatattt
     5521 tgacagaaaa aagattaatg tgaaatacgt atgtatgaca atgtatgata agactattga
     5581 aaatgtggaa attgcttaca agcctaattt tgtgcaaaat accttaggtg aatatttaag
     5641 cagactgggc attaagcagt taagagttgc agaaacggaa aaatatgctc atgttacata
     5701 tttttttaac ggtgaaatag aagaaccttt tgaaaatgag gtaagaattt taataccttc
     5761 acctgatgtg cccacttatg acttaaagcc cgaaatgagt gcttttgagg taaaaaacac
     5821 tgttatggag caattagaaa aggatatgta taaggttatg ataattaatt ttgcaaatcc
     5881 tgatatggtg ggacacacgg gagatataga ggcagctata aaggcagtgg aagcagtgga
     5941 taaatgtttc ggtgaaatag taaactatat aataagggcg gaaggaacag caataattac
     6001 atcagaccat ggaaactgcg aagaaatgtt tgacagagaa acccttgata taatcacttc
     6061 tcacaccggc aacagagtgc cctttatagt tgttgacaac aaaaatttca aattaagaga
     6121 aggaattctt gcagatgtag cccctacgat tcttgagctt atgggactcg aaaaacctgc
     6181 agaaatgaca ggaaaaagtt tgatagttca taatgaataa aggagaatag tatgtcaata
     6241 ataacagata tttatgcaag agaaatattg gattccagag cgaatcctac aattgaagta
     6301 gaagtatgga cagaaagcgg cgggtacgga agggcaggcg taccatccgg agcttcaaca
     6361 ggagcatttg aagctg
//
LOCUS       DAOSHP010000092         6364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_10723_length_6364_cov_8.223173, whole genome shotgun sequence.
ACCESSION   DAOSHP010000092 DAOSHP010000000
VERSION     DAOSHP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10723_length_6364_cov_8.223173"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..512
                     /locus_tag="PLB33_08020"
     CDS             174..512
                     /locus_tag="PLB33_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63509.1"
                     /translation="MYNENKFEKGHPYCPPVYGNETCNTTEIKEILEVLVPVTVAPEV
                     EVGNAAVTVVGEPCVRPLPCSSLSRNGICKFLVTQKLCVQVPITFNATARANDGLSTD
                     CFENRCPRQC"
     gene            626..973
                     /locus_tag="PLB33_08025"
     CDS             626..973
                     /locus_tag="PLB33_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63510.1"
                     /translation="MNSKDNKNTSNSFSGGIFVTADELNNMNFLSGKQLGDGKEQFSN
                     IKQWTKSMKDIEAEQKNKQEIIAEIRKLEEKIDYVVKKLEQIEPNTGTNAGINNATNY
                     KTNSTWKDKIKMW"
     gene            complement(1014..1715)
                     /locus_tag="PLB33_08030"
     CDS             complement(1014..1715)
                     /locus_tag="PLB33_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63511.1"
                     /translation="MACDLIPRLNSLSTEFGITRELNEERDLRCVDICVPHVLLIHAF
                     DPFEKFIPWPNATTNPNRDCRGVFDIPPGLPPENCFFRVTCANEEFNCVEGVPTITVT
                     VEGQLVLEFVVNGTSIFWVKEITVLDETLNAASNWYRTDTGNEIVDTGFAFFMTLIDG
                     SCIVIDSQCEIDTEGQQVIFTANVVDKLWKHENVWVEGVTPYLQLRFNQPPTATNITV
                     HEEFVDHAIPSCIIP"
     gene            complement(1815..2387)
                     /locus_tag="PLB33_08035"
     CDS             complement(1815..2387)
                     /locus_tag="PLB33_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOG63512.1"
                     /translation="MSSITLIFTSLGLSMDACAVSISNGMCYGNIHKKQIITTAAAFG
                     FFQAFMPVLGFFIGSAFSNAISFLDHWIALVLLGFIGVKMIVEAVRELKYPEVCITGA
                     KHLTSKTLFFQALATSIDALAVGIGFAVMKVNIFSAALFIGIITFVNSIIGSNLGKKC
                     GQLFKQKAEILGGVILVGIGIKIFLEHTLV"
     gene            2692..4419
                     /locus_tag="PLB33_08040"
     CDS             2692..4419
                     /locus_tag="PLB33_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOG63513.1"
                     /translation="MVYLLIAFGVFILIEGLIIYKLIMKNNKRLFSQPINAEKIINKE
                     NDLSGKYLELISRFSFSVEEIYMEMKEISGKVENIISESEEQTASMISISEVIENIYN
                     NVEINLAGSQTSSEISKKTHEEIYKKIMQLQDNIDELSRVRSLLDNISNLIADLETKT
                     LNAESLISRINNISQQTNLLAINASIEASRAGEYGKGFSVIAVEIRSLAAETNEVSNV
                     ISEIIKELRSSFVNTKKELIDVLDKIEEQTISIDKTIVSFKNIENISKELYNKTSDIS
                     FNSQKIVEQIYNLKEFINSTTLSVEEVTKSIVDVNQSVLLETKSVDNLNSSISKFEEV
                     NFEFLQLVKDYYPDNRIIIATMPYEPFIIYDIEKNIVSGIDIDMINEIYSEAGYQVEY
                     KIVPWETSINMAKKGLVDILPTIEINEEREEFLDFSINYRNESRYNFYTSKENNISIG
                     NIDDLSGKRVGVLTGYKYYAEFDQNTSFDRNYSFKEEIMFQKLLKGQIDVVILNSYSG
                     NYLFNKLNLVDRVKEEKYKYAHPDTSESRIGFVKAKNNKHLLDLFNKRFKELEKDGTI
                     KKITEKYLT"
     gene            4561..6111
                     /locus_tag="PLB33_08045"
     CDS             4561..6111
                     /locus_tag="PLB33_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOG63514.1"
                     /translation="MTYSPALGSTISNTKMRTPENVSPYSGMCSVCTANCIGTCEIGL
                     SAVRGSEATFPYRRDINQFASEKDYPLDFSHLSINGRVFGALGCEEDANKATYTNVKT
                     ETEFGIKNKVKMKMPIILPAIAKLNWKDYFVGAALAGVSVVIGEDAIPNDKNLVLENG
                     KVVSAPLVGEMVDMFRKYSRGYGEIIMQANYDDENSGVLDYVIPKLGVKSVELKFGQA
                     AKGIQGMGRTNSLEEALKLQNKGYLVYPDPSDEKVAENFKNGKGPIFEKIGKLPIWNE
                     EILKNRIAHLRELGAERICFKTGPFDPKDLIRILKIASENEVDLVTFDGAGGGTGNSP
                     VKMMNEWGMPTVYLESMLYDILKRMDKKGYFLPQVAITGGFTLEDHIFKGLALGAPYI
                     QFVAVGRAAMAAAMVGKQVGELIEAGNIPKEYQSFGSTKEEIFADIRELKLYYDNAQD
                     LPTGAVGVYSYISRIAAGIKQLMALNRKFDIKYIDRSDIFPLTDLAAQVTGLDTYEDV
                     YVRELEKL"
BASE COUNT         2438 a          898 c         1122 g         1906 t
ORIGIN      
        1 cattaactta tgtggcggct atggcaagcg gtttggcaca gctcatgaga ttaattctta
       61 tcagaaaccg gagaagataa ttaatagaaa aaattaaaac tatatgatat tttgtaacca
      121 agccggcatg tcttcataat atatattaaa acaaaatatt ggagtttgat aatatgtata
      181 atgaaaataa atttgaaaaa gggcatcctt attgccctcc cgtatatgga aatgagacat
      241 gtaatactac agaaataaaa gaaatattgg aagtattagt accggtaacg gttgccccag
      301 aggtagaagt tggtaatgct gctgtgaccg ttgtaggcga gccgtgcgtt agaccgcttc
      361 cttgctcaag cttgagcaga aatgggattt gtaaattttt agtaactcaa aaactgtgtg
      421 tgcaagtgcc tatcactttt aatgccactg caagagcaaa tgacggatta tcaacagatt
      481 gttttgaaaa tagatgccca agacaatgtt gattcaaatc aaaatttata tataagttga
      541 attataaaag ctccggtttt ttctggagtt tttatatact gaatcaatca taatggaaga
      601 tgtctattgc atggaggtgt aataattgaa tagtaaagat aataaaaata cttccaacag
      661 cttttcagga ggaatatttg taacagcaga tgaactaaat aatatgaatt ttttaagcgg
      721 caaacaatta ggtgacggca aagaacaatt cagcaatatt aagcaatgga ccaaaagtat
      781 gaaagatata gaagcagaac aaaaaaataa gcaggaaata attgctgaaa taagaaaact
      841 agaagaaaaa attgattatg tagtaaaaaa acttgaacaa attgaaccca atacaggaac
      901 caatgccgga atcaataacg caactaatta taaaactaat tctacatgga aagataaaat
      961 aaaaatgtgg taaacagata aaaaatagcc agaattttct ggctattata ttatcatgga
     1021 ataatacaac ttggtattgc gtgatcaaca aattcttcat gcactgtgat attagtagct
     1081 gtaggaggtt gattgaatct tagttgcaga taaggtgtta cgccttctac ccatacattt
     1141 tcatgcttcc ataatttatc aactacattt gctgtaaata taacttgttg cccttctgta
     1201 tcaatttcac attgtgaatc aattactatg catgatccat ctattaaagt cataaagaaa
     1261 gcgaaaccag tatcaacaat ttcattacct gtatcagttc tgtaccaatt agacgcagca
     1321 tttaaagttt catcaagtac tgtaatttct tttacccaga atatagaggt gccgttaaca
     1381 acaaattcta atacaagctg accttcaacg gttactgtaa tagtaggcac tccctcaacg
     1441 caattaaatt cttcatttgc acaagttact ctaaagaaac agttttcagg cggtaacccc
     1501 ggtggtatat caaatacgcc cctgcaatca cggtttggat ttgttgttgc atttggccaa
     1561 ggaataaatt tttcaaatgg atcaaatgcg tgtattagta aaacatgagg tacacaaata
     1621 tctacacaac gcaaatctct ttcttcattt aattccctcg ttattccaaa ctccgtacta
     1681 agactgttaa gacgaggtat taaatcacat gccataaaat catctccgaa aatatttatt
     1741 aaaccataca taatctatgg ttaatatata atatgacttg tctcaataaa tgttctcaaa
     1801 ttaatcaaat ttttttatac caaagtgtgt tctaaaaaaa ttttaattcc tattcctacc
     1861 aagattactc caccgagaat ttctgctttt tgtttaaaca attgcccaca ttttttccct
     1921 aaattagaac ctattataga attaacaaaa gtaattatac ctatgaacag ggcagcactg
     1981 aaaatgttca ctttcataac ggcaaatcct atacctacag ctaatgcatc tatacttgtt
     2041 gctaaagctt gaaagaacaa tgtttttgat gttaggtgtt ttgcacctgt tatgcatact
     2101 tccggatatt taagttcccg gactgcttca acaatcattt ttacgcctat gaaaccaagt
     2161 aaaaccagtg caatccaatg gtctaaaaat gatatggcat tactgaatgc tgagcctata
     2221 aagaaaccta aaacaggcat aaatgcttga aagaaaccga aagcagccgc agttgtaata
     2281 atttgttttt tatgtatatt tccgtagcac ataccgtttg atattgatac ggcacaagcg
     2341 tccatggata atcctaagga tgtaaatatt agtgttatgg aactcatttt gcctccaaag
     2401 tttatagctc atattattga gtttttctat atggtttata actatattat aatttgttta
     2461 atacaaaaat tatatcattc aatattgttt atttcaaggc tattgtgtag aaagtgacag
     2521 ccaactaact ttcaagttag tgacataaag gataatccgt gaaaagtaca ttgacataaa
     2581 tagtcaatta ttgtatattt atatcaataa ttactttaca aggggctagg caagacataa
     2641 ataaaatgca aaattctata tggattcaaa aatcttggag gaattaaaat tatggtatat
     2701 ttattaattg catttggagt gtttatttta attgaaggtt taatcattta taaactaatt
     2761 atgaaaaata ataaaagatt atttagtcaa ccaattaatg cagaaaaaat tattaacaag
     2821 gaaaacgatt tatccggcaa atatttagag ctcataagca ggttctcatt ttcagttgag
     2881 gaaatatata tggaaatgaa ggaaatatca ggtaaagttg aaaatataat atcagaatca
     2941 gaagaacaga cagccagtat gatatccata tcagaagtaa tagaaaacat ttataataat
     3001 gttgaaataa atcttgccgg ttcacaaact tcatctgaaa tatcaaaaaa aactcatgaa
     3061 gaaatataca aaaaaataat gcagcttcaa gacaacattg atgaattatc aagagtaaga
     3121 agtttattag ataatataag caatttaatt gcagatttag aaacgaagac tttaaatgcg
     3181 gaatccttaa taagtagaat aaataatatt tcacagcaaa ctaatttatt agcaataaat
     3241 gcatctattg aagcttcaag agccggcgaa tatggtaagg gtttttctgt aatagcagtt
     3301 gagattaggt cattagcagc tgaaactaat gaagtttcta atgttatatc tgagataata
     3361 aaagagttaa ggagttcatt tgtcaatact aaaaaagagt taatagatgt tcttgacaaa
     3421 atcgaagaac aaactataag tattgataaa accattgtaa gttttaaaaa tatagaaaac
     3481 atatccaagg agctatataa taaaaccagt gatatatcat ttaattcaca aaagattgtt
     3541 gaacaaatat ataatcttaa agaattcatt aattcaacaa cactttcagt tgaagaagta
     3601 acaaaatcaa ttgttgatgt taatcaatca gtattattag aaactaaatc agtagataat
     3661 ttaaatagca gtatcagtaa attcgaagaa gtaaattttg aatttttaca attagtaaag
     3721 gattattatc ctgataacag gattattata gctacgatgc cttatgagcc atttataatt
     3781 tatgatattg aaaaaaatat tgtttcggga atagatattg atatgattaa tgaaatctat
     3841 tcagaagcag gttatcaagt tgaatataaa atagtaccat gggaaacttc tattaatatg
     3901 gccaaaaagg gtttagtaga tattcttcct acaatagaaa ttaatgaaga aagagaagaa
     3961 tttttggatt tttctattaa ttatagaaat gaaagcagat acaattttta tacaagcaaa
     4021 gaaaataata tttctattgg caatatagat gatttaagcg gtaaacgtgt aggggtatta
     4081 acaggatata aatattacgc agaatttgat caaaacacct ccttcgatag aaattatagc
     4141 tttaaagagg aaataatgtt tcaaaaatta ttgaaagggc aaatagatgt agtaattttg
     4201 aattcgtatt ccggaaatta cttatttaat aagttaaatc ttgttgatag agtaaaggaa
     4261 gaaaaataca aatatgctca cccggataca agtgaatcaa gaatagggtt tgttaaagct
     4321 aaaaataaca agcaccttct tgatttgttt aataagagat ttaaagaatt agaaaaagac
     4381 ggcacaataa agaagataac agaaaaatat ttaacttgag tcataatgat agtctagaaa
     4441 actgagtgct cacattaact ttgagatagc ataaataaaa tatagaaaaa tcaaatatat
     4501 tattgacata tgtcacaata gaggtataat taatataaac aatgaattag gaggtttatt
     4561 atgacttata gccctgcttt aggaagtacg ataagcaata caaaaatgag gacgccggaa
     4621 aacgtttctc cgtattccgg aatgtgttca gtatgcacag ctaattgtat cggtacctgt
     4681 gaaataggat tatcggcagt gagaggctca gaagcaactt ttccatatcg tagagatata
     4741 aatcaatttg cttcagaaaa agattatccg ttagactttt ctcatctatc tattaatgga
     4801 agagtattcg gagcattggg atgtgaagag gatgctaata aagctacata cacgaatgtg
     4861 aaaactgaaa ctgaatttgg cataaaaaac aaagtaaaaa tgaaaatgcc gataattttg
     4921 cctgccattg caaaattaaa ttggaaagat tactttgtag gggctgctct tgcaggagta
     4981 tcggtggtaa tcggcgaaga tgctattcca aatgataaaa atttagtatt ggaaaacgga
     5041 aaagtagttt cagctcctct agttggagaa atggtagaca tgttcaggaa atactccaga
     5101 gggtatgggg aaatcattat gcaggcaaac tatgatgatg agaattccgg agtattagat
     5161 tacgttatac caaaattagg cgtaaaatcc gtggagctta aattcggcca agctgcaaaa
     5221 ggaattcaag gaatgggaag gactaattca ttagaggaag ctctaaaact tcaaaataaa
     5281 ggatatttgg tgtatcctga cccatcggac gaaaaagttg ctgaaaattt caaaaatgga
     5341 aaaggaccta tatttgaaaa aatcgggaag cttcctatat ggaatgaaga aattctcaaa
     5401 aaccgcattg cacatttaag agaattggga gcagaaagga tttgctttaa aacaggtccc
     5461 ttcgacccaa aagacttaat caggatttta aaaattgcat cagaaaatga agtggatttg
     5521 gttacatttg acggtgccgg aggaggcaca gggaacagcc ccgttaaaat gatgaatgaa
     5581 tggggaatgc ctaccgttta tttggaaagt atgctgtatg atatactgaa acgaatggat
     5641 aagaaaggat atttcttgcc tcaagttgca attacgggag gatttacttt agaagaccac
     5701 atatttaagg gattggctct aggagctcca tatattcaat ttgtagctgt cggaagagcc
     5761 gcaatggctg cggccatggt aggaaaacaa gtgggcgaat tgattgaagc aggcaatata
     5821 cctaaagaat atcaaagttt cggaagcaca aaggaagaaa tttttgctga cataagagaa
     5881 ttaaagcttt attatgacaa tgcacaagac ttaccgacag gagccgtagg agtgtattca
     5941 tatataagca gaatagctgc aggaataaag caattaatgg ccttgaaccg taaatttgac
     6001 ataaaatata tagacagaag cgacatcttc ccattgactg atttagcagc acaagtaaca
     6061 ggcttagata catatgaaga tgtttatgta agagaattag aaaaactgta ggaacaaata
     6121 ccatttgtca tcctgagcgt aagcgaagga tctcagggtt taaaaaatga gattcttcga
     6181 gcttaagctc tcagaatgac acatagctta ttcatagcaa tgataaaaat gttaatcatt
     6241 gcaagacact aacatgtatt catataaaat aattaaaact gcaaaaattt ttttgcagtt
     6301 tttttaaaaa agttattgac atatgtcgga tatagtgtat aatataagat aaatgacata
     6361 tgtc
//
LOCUS       DAOSHP010000093         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_10985_length_6258_cov_10.341931, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000093 DAOSHP010000000
VERSION     DAOSHP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10985_length_6258_cov_10.341931"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..542
                     /locus_tag="PLB33_08050"
     CDS             84..542
                     /locus_tag="PLB33_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOG63515.1"
                     /translation="MRIIIDGDACPQKVREICEKAAKDFGVGLIIVSDIDHYIVSDFE
                     VIVVEQGRDSVDYKIVQIFQQGDILITQDYGLASLLLGKASAIIHTAGFFINKNNIDS
                     LLQSRYISDKIRKQGGKSKGPSKRRKEQDENFKKCLYKVLREYMSNKKGR"
     gene            complement(600..1697)
                     /locus_tag="PLB33_08055"
     CDS             complement(600..1697)
                     /locus_tag="PLB33_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOG63516.1"
                     /translation="MDKNILITTAPFGNGHKMVATAIKNAFIKKGYNNVVVVDLFTET
                     HPKITEAIKKAYIRSYDFGKAYSKLYYGSEKLTDKKVVELYRNFGFSKLKDISEVFKP
                     DVIINTFPILASLKLKNEQGENIPVFNIVTDYYAHKLWISKEIDKFYIATYELQEEFK
                     KMNIPPEKIVVSGIPIRDAFEEIDNISKLHAKYKFNRRKRIILINSGAFGVLKDIKKV
                     CTELCKNKNIQVAVVCGNNIYQKTKLESLRLRNLKVFGFVEDIHELYKIACCMITKPG
                     GITLTEALSVQLPLIIIKPVPGQEKENARYFEKKGAAIIANNTYQIINSAIDLINNPE
                     LLSAMRKNMKKMYNKSSSDRIVDDVLGSLEE"
     gene            1802..2353
                     /locus_tag="PLB33_08060"
     CDS             1802..2353
                     /locus_tag="PLB33_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOG63517.1"
                     /translation="MYKEFLETFILVFVAEMGDKTQLMLMTLAAKYKIIDVLLGIFLG
                     VLLNHGTAVFIGSCISNLIDEYLLHVFAGVVFIIFGIITIVFEDEDENKKQYGLGPVI
                     TTALTFFLGEMGDKTQLTCMTLSMDAYYPSAVLAGSVTAMLAIGLAGIIIGTSLTKFI
                     PSYIIKTISGLIFIIFGVVRMII"
     gene            2354..2998
                     /locus_tag="PLB33_08065"
     CDS             2354..2998
                     /locus_tag="PLB33_08065"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOG63518.1"
                     /translation="MNLKNIKDKLKNIKPKPIDFDVSFAVLVPIIEIDGELNLIFEVR
                     SNSITQPGEISFPGGRIEEGESPRKAAIRETSEELLLNKENIEIISELNYASSKSGAF
                     VFTFLGLIKDVDPDEIEYSIDEVSEVFFVPLSFFLENRPEKYYMNYYPKADKDFPYHM
                     VNNGEDYNWGNIRYPVYFYKYYNYIIWGLTAKITYSLIKELTNSDVKKRNNNES"
     gene            2988..3884
                     /locus_tag="PLB33_08070"
     CDS             2988..3884
                     /locus_tag="PLB33_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOG63519.1"
                     /translation="MKASIFNIQKFSIHDGPGIRTTVFFKGCPLQCIWCHNPESQNLG
                     KEILYDKNKCTLCGSCIKICQNNAIELKDNDLEINMDKCTFCGDCTVCCINSAKQIAG
                     KEYTVDEVMKEVLKDRVFYKNSKGGVTLSGGEPLIYAAFVEELLMELKKENIHTAVDT
                     CGAVDFKVLERISKYTDLFLYDIKSMDEEKHILYTGVSNKNIINNLINLSKIHNNINL
                     RLPIIEGINADENHIFEILKLIKNTNIKKINLLPYHDIAMHKYEKLGRKYYEYMKRPA
                     DEKLKRYIDIIEKEGYRVKIGG"
     gene            3886..6258
                     /locus_tag="PLB33_08075"
     CDS             3886..6258
                     /locus_tag="PLB33_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOG63520.1"
                     /translation="MRGYTERTKRLREISTRTQPSVSLERALIETSVYEKYYGTVEIP
                     VLRALNFKNLMEKRKLYIGEDELIVGEKGEGPQVAPTFPELCCHTVEDMTVMNDRKYI
                     NFKVKEEDKKLQQEKIIPYWEKRSIRHKILESMTQEWKDCYAAGMYTEFMEQRAPGHT
                     VADGKIYEKGFLDFKNEIEEEIEKLDFMNDPDAYDKKAQLEGMAISCDAIIIYGQRYA
                     QYARELASKESNPKRKEELLWIANNCDVVPAHKPETFAQALQMYWFVHIGITTELNTW
                     DSFSPGRLDQYIYPFYKKEKEEGTIDYHKARELLECFWIKFNNQPAPPKVGITLKESG
                     TYTDFANINTGGINPYTGEDGVNEISYMILDVMDELKLLQPSSNVQISVKTPDEFLKR
                     AVEISRKGWGQPAFYNTEELIQELLNAGKTLEDARFGGASGCVETGCHGREAYVLTGY
                     LNVPKVLELTLNNGFDRYTNKQIGLKTGNPEDFKTYEELYDAFVKQLKYIIDVKIRGN
                     NVIEKIFAEHMPSTFMSVLTDGCKESGRDYNAGGSKYNTRYIQVVGIATITDCLSSIK
                     YNVYDNKRFTMGELLKALEANFEGYAHIKNLVSNHTPKYGNDDDYADDIMIDIFTDVR
                     DLIKGRKTVCGGTYQIDMLPTTCHVYFGSVMGASPNGRLAKKPVSDGISPEKGADVNG
                     PTAVIKSCSKMDHTSTGGTLLNQKFSPSSIAGEEGIENVSSLIRSYFELMGHHIQFNV
                     IDKKTLLDAQKHPEEYKDLIVRVAGYSDHFNNLEKSLQDEIIERTEQSFN"
BASE COUNT         2212 a          942 c         1223 g         1881 t
ORIGIN      
        1 tgcaatgacg gatttataaa attatttgca actgttaaca aaaaagatat aatatcataa
       61 cttaattaaa aaaggagttt ttcatgcgaa ttataattga tggagatgca tgcccgcaga
      121 aggtaagaga aatatgtgaa aaagcagcca aggattttgg agtgggcttg ataattgttt
      181 cagacattga ccattatatt gtcagtgatt ttgaagttat tgttgttgag cagggaagag
      241 attccgttga ttataaaata gttcaaatat ttcagcaagg agatatatta ataactcaag
      301 actacggact tgcaagcctt cttttgggga aagcatcagc aatcattcat acagcagggt
      361 tttttataaa taagaataat attgacagcc tgcttcaatc cagatatata agtgataaaa
      421 taagaaaaca aggcggaaaa tcaaagggac cgtcaaaaag aagaaaagaa caggatgaaa
      481 actttaaaaa atgcctgtat aaagttttaa gagaatatat gtcaaataaa aagggcaggt
      541 aattctaaac gaaaaaaatc atatcatagg aagggggaca taaagtaatg tcccttactt
      601 tattcctcta agctccctaa cacatcatca acaattctgt ctgatgaaga tttattgtac
      661 attttcttca tgtttttgcg cattgcggac agaagttcag gattatttat taaatcaata
      721 gcgctgttta taatttgata agtattgttg gctattattg ctgcaccttt tttttcaaaa
      781 tatcttgcat tttctttttc ctgaccgggt acaggcttga ttataataag aggcagctgt
      841 accgataatg cttccgtcaa tgtaattcca cccggtttgg taatcataca gcatgcaatt
      901 ttgtatagtt catgaatatc ttcaacaaac ccgaacactt ttagatttct gagcctcaag
      961 gattccaatt ttgttttctg atatatattg ttgccgcaaa ctactgctac ctgaatattt
     1021 ttatttttgc acaactccgt acatactttt ttaatatcct ttaatacacc aaatgcgcct
     1081 gagtttataa gaattattct ttttcgtctg ttaaatttat atttggcatg gagtttcgaa
     1141 atattgtcaa tttcttcaaa agcatccctg atgggtattc cggatacgac tattttttcc
     1201 ggaggtatat tcattttttt gaattcttcc tgcaattcgt atgtcgcaat ataaaacttg
     1261 tcaatttcct tgcttatcca aagtttatga gcataatagt cggttactat attgaaaaca
     1321 ggtatatttt ctccttgttc attcttcagc tttaaagatg ccaaaatggg gaaagtatta
     1381 attataacat caggtttaaa tacttcagat atatccttta attttgaaaa tccgaaattt
     1441 ctgtataatt caactacctt cttatccgtt agtttttctg agccataata aagttttgag
     1501 taagcttttc caaaatcata gcttctgata tatgcctttt ttatagcttc ggtgattttg
     1561 ggatgtgttt cagtaaacaa atctacaaca accacattgt tatacccctt tttaataaat
     1621 gcatttttta ttgcagttgc aaccatttta tggccgtttc caaagggggc ggtggttatc
     1681 aatatatttt tatccataat acctcccgaa atgattaatg ttgttctgtt tgtacgtcgc
     1741 ttttaatatt ataatatata actcggataa aagaaagaga gtatattgtc agggagggaa
     1801 aatgtataaa gaatttctgg agaccttcat cttggtcttt gttgctgaaa tgggagataa
     1861 aacccagctg atgcttatga ccttggcagc caagtataaa ataattgatg tgcttttagg
     1921 aatttttctc ggtgttttat taaatcatgg aactgcggtc tttataggca gctgtatctc
     1981 taatttaata gatgaatatc ttcttcatgt atttgcaggt gttgttttca taatttttgg
     2041 aattattact attgtatttg aagacgagga cgaaaacaaa aagcaatacg gcctgggtcc
     2101 tgttattacc actgctctta ctttttttct cggagaaatg ggagacaaaa ctcagcttac
     2161 atgcatgacc ttatccatgg atgcatacta cccttcagca gtattggcag gctccgtaac
     2221 agcaatgctt gcaataggtt tggcaggaat tattatcgga acttcattga ctaaatttat
     2281 tccttcctat ataataaaaa caatatccgg actgatattt ataatattcg gagtagtaag
     2341 gatgataata taaatgaatt taaaaaacat aaaagataag cttaaaaata taaaaccgaa
     2401 acccatagat tttgatgtaa gctttgcagt attggttccc ataattgaaa ttgacggaga
     2461 attaaactta atattcgagg ttagatcaaa ttccattact caaccgggag aaatttcgtt
     2521 tccgggagga agaattgaag aaggtgaaag cccaaggaaa gctgcaataa gagaaacttc
     2581 agaagagcta ttgttaaata aggaaaatat agaaataatt tcagagctga attatgcttc
     2641 aagcaaaagc ggggcttttg tatttacatt tttagggctg attaaggatg tcgacccgga
     2701 tgaaatagaa tacagcattg atgaagtttc ggaagttttc tttgtaccct tgtcattttt
     2761 tttagaaaat cggcctgaaa aatattatat gaattattat ccaaaggccg acaaggattt
     2821 tccttatcat atggttaaca acggggaaga ttacaattgg gggaatattc gatatcctgt
     2881 ttatttttac aagtactaca attatataat atgggggttg acagctaaaa taacttacag
     2941 ccttattaaa gagcttacaa acagtgacgt caaaaagagg aataataatg aaagctagta
     3001 tattcaacat tcagaaattt tctatacatg acggacccgg aataagaacg acggtattct
     3061 ttaaaggatg ccccttacag tgcatatggt gccataatcc ggaaagccaa aatttaggta
     3121 aagaaatttt atacgacaaa aataaatgca cattatgcgg atcttgtatt aagatatgtc
     3181 aaaacaatgc tatagaatta aaagacaatg accttgaaat aaatatggac aagtgtactt
     3241 tttgcgggga ttgcactgtt tgctgcataa actccgcaaa acagattgcc ggaaaagaat
     3301 ataccgttga tgaagtaatg aaagaagtac taaaagacag agtattttat aaaaactcaa
     3361 agggcggtgt aacattgtcc ggaggagagc ctcttatata tgcagccttt gtcgaggaat
     3421 tgttaatgga gttaaaaaaa gaaaatattc atacagcagt tgatacttgc ggagcggttg
     3481 actttaaagt gttggaaagg atatctaaat atacggactt atttctatat gacataaaat
     3541 ccatggatga agagaagcat atactatata cgggagtttc aaacaaaaat ataattaata
     3601 atttaattaa tctgtctaaa attcataaca acataaattt gagattacct ataattgaag
     3661 gaatcaatgc cgatgaaaat cacattttcg aaatattaaa attaattaag aatacaaata
     3721 ttaaaaaaat taatttgctg ccttaccacg atatagcaat gcataaatat gaaaaactgg
     3781 gtcgaaagta ttatgagtac atgaaaagac ctgcagatga aaaattgaaa cgatatatag
     3841 atataattga aaaagaaggt tacagagtca aaataggagg ctgacatgag aggatacact
     3901 gaaagaacca agagattaag agaaatcagc acaagaacac aaccatcggt tagtttagag
     3961 agagctttaa tagagacttc agtttatgaa aaatactacg gtacagtgga aattcctgta
     4021 ttgagggcgt taaattttaa aaatttgatg gaaaaaagga aactttatat aggggaagat
     4081 gaactcatag tcggagaaaa aggcgaaggg cctcaagtag cccccacatt tccggagctt
     4141 tgctgccaca ctgtcgaaga tatgaccgtg atgaatgaca gaaaatatat aaactttaag
     4201 gttaaagaag aagataagaa actgcagcag gagaaaatta tcccatattg ggaaaagcgc
     4261 tccataagac ataaaattct tgaatcaatg acacaggaat ggaaggattg ttatgctgca
     4321 ggaatgtaca ctgagtttat ggagcaaaga gctcccgggc atacagttgc agacggaaaa
     4381 atttacgaaa aaggatttct ggattttaag aatgaaattg aagaagaaat tgaaaaactt
     4441 gatttcatga atgaccctga tgcctacgac aaaaaggcac agctggaagg tatggccatt
     4501 tcatgtgatg caataataat ttacggtcaa agatatgcac agtatgcaag agagttagct
     4561 tctaaagagt caaatccgaa aagaaaagaa gaattattgt ggattgcaaa taactgtgac
     4621 gtagtgcctg ctcataaacc tgaaactttt gcacaagctc tgcagatgta ttggtttgtg
     4681 catataggca taacaacaga gcttaatact tgggactcct tttcgcctgg aagactagat
     4741 caatatattt atcccttcta taaaaaggaa aaggaagagg gtacaattga ttaccataag
     4801 gcaagggagt tgttggaatg cttttggatt aagtttaaca accagcctgc tccccccaag
     4861 gtaggaataa cattgaagga aagcggaact tatacggact ttgcaaatat taacacgggg
     4921 ggaataaacc cctatacggg agaagacggt gtaaatgaaa tatcttatat gatacttgac
     4981 gtaatggatg agttgaagct tctccaacca agctcaaatg tccagataag tgttaaaact
     5041 cccgatgaat ttttaaaaag ggctgttgaa atttcaagaa aaggctgggg tcagccggca
     5101 ttttacaata cggaggaatt gattcaggag ttgttgaatg caggcaagac attggaagat
     5161 gcacgatttg gtggagccag cggatgtgtt gaaacaggct gtcatggaag ggaagcctat
     5221 gttttaacag gttatttgaa tgttcccaaa gtattggagc ttacattgaa taacggcttt
     5281 gacagatata caaataaaca gattgggtta aaaacaggaa accctgaaga ttttaagaca
     5341 tatgaagagc tttatgatgc ttttgtaaag cagcttaaat atattattga tgttaaaatt
     5401 agaggtaata atgtaattga aaaaatcttt gcagagcata tgccttcaac atttatgtca
     5461 gtgctgaccg acggatgcaa ggaaagcggc agggactaca atgcaggagg ctcaaagtac
     5521 aataccagat atattcaagt tgttggaata gctactatta ccgattgttt atcttcaatt
     5581 aaatacaatg tatatgataa taaaagattt acgatgggtg aattgttaaa agcattggaa
     5641 gcaaattttg aaggatatgc tcatataaaa aatttggtat caaatcacac gcccaaatac
     5701 ggcaatgatg atgattatgc tgatgatata atgatagata tatttactga cgtaagagat
     5761 ttgataaaag gaagaaagac tgtgtgcggg ggtacatatc aaattgatat gcttcctact
     5821 acttgccacg tatatttcgg ctcggtaatg ggtgcttctc caaacggaag gttagcaaaa
     5881 aaacctgtgt cagacggtat atcccccgaa aaaggtgcag atgtgaacgg ccctactgcg
     5941 gtaatcaaat catgctctaa aatggaccat acatctacgg gaggaacctt gctgaatcaa
     6001 aaattttcac cttcatccat agcaggcgag gaaggaatag aaaatgtatc ttcactaata
     6061 aggtcatatt ttgaattgat gggtcatcac attcaattta acgtaattga taaaaagact
     6121 cttttagatg cacaaaaaca tccggaggaa tacaaggatt taatagttag ggtagccggt
     6181 tactcggacc actttaacaa tcttgaaaaa tctttgcagg atgaaataat tgaaagaaca
     6241 gagcagtctt tcaattaa
//
LOCUS       DAOSHP010000094         6007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_11633_length_6007_cov_6.285114, whole genome shotgun sequence.
ACCESSION   DAOSHP010000094 DAOSHP010000000
VERSION     DAOSHP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11633_length_6007_cov_6.285114"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1576)
                     /locus_tag="PLB33_08080"
     CDS             complement(77..1576)
                     /locus_tag="PLB33_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOG63521.1"
                     /translation="MEFFQYAIQAATPENMLWLVIGCIIGAILGALPGMSADTGLAIF
                     IPLTFNLDPTTALITLGAIYVTGAYGGNITAVLVNTPGTSDSVFMTIDGYPMTQRGEG
                     LKAIGVTTFSSFFGGMVGAASLIFLAPIIAKMAVLFGPWELFLVTMMGMVVILGMVKE
                     GVLKGLVSASFGFLCSLIGMDGVTGLTRLSFGIKPILDSLPLLPVVLGLFAVSQMFDL
                     AATHSATIAITDSAIKGSPFLSKKEHKAMFKNNLRSSIVGTIIGIVPGAATTAAAGIS
                     YNMAKKDDPHPETFGKGNPQGLACVSAANNAEVTGALIPLLTLGIPGNGTSALFLGAL
                     LVHGLAPGTQLFAKTPEIAYGMFFGIIMATFAILLIGLYGAPIYSKITKVPNSILIPI
                     IGSLCVLGAFTYRNLFFDTLLILVFGIIGFYMQRSNFPLAPFVLTFVLGKTSEMAFRR
                     TMIITGGDLTSVMFKPLALALFAIDLILLIWPFWGDIKDYLKKDKTKTI"
     gene            complement(1614..2069)
                     /locus_tag="PLB33_08085"
     CDS             complement(1614..2069)
                     /locus_tag="PLB33_08085"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOG63522.1"
                     /translation="MENGYNKKFNSNYLSAAFIYLVSISFLINSFSIKDPQSRMFPTV
                     ICITTIILATLLVIRTKFMTTKKADEDNLTGMTTALKMLALLFLYVVGIEIVSFYIAT
                     PIYLSFTMLALGQKNKKLVVIVSLLFTLGVYLFFDLLLGMAVPMGRFLS"
     gene            complement(2148..3188)
                     /locus_tag="PLB33_08090"
     CDS             complement(2148..3188)
                     /locus_tag="PLB33_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOG63523.1"
                     /translation="MIKRKTISIIALLLVILLSLAMAGCSQKEEPAEKPSENKEGVVP
                     RPEGLPEDYPNKEITFIYAFSAGSPLEAYIRILGDKMQEMQGWKNGVVISFKEGASGR
                     IGWNASAEAEPDGYFTGFAPSAMLISAVSEDVSYGVDKLDFIFNTMSDPGAIGVITGS
                     PYKTLGDLVQAAKENPGKISVGVTSVIGQEGLTIKMIERAAGVDFNEVAFDGEGAIFA
                     AVLGKHLDAFCLNVSDATSFIEDKSITILATGDSDRSPFLPDVPTYKEAGYDVTQVNM
                     RAVSCPKGTPEPIRKYLEQCFIAAANDPEVAAKAAEMKLPIDTLPGDEVKAIFTQISE
                     GYAKLWAEDPWN"
     gene            complement(3428..4675)
                     /locus_tag="PLB33_08095"
     CDS             complement(3428..4675)
                     /locus_tag="PLB33_08095"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOG63524.1"
                     /translation="MNRYTEILKEELIPAFGCTEPIALAYAAAKATQVLGHIPERIIC
                     SCSGNIVKNAKSVVVPGTDGLKGIEISVVLGAIAGDPDKELEVLNGIEKNKVAEAKKL
                     AEEGMCQIKLVPNVANLYIKIQSFYEDECAAVTLKDGHLNIIELHKNNDLLYKKDSNS
                     EEEDDDIEIFRFKNIYNYANNVNIEEVKLILDKQIEYNLNIAKEGLENDYGARVGKTI
                     YSKSPNDINEIAKAYAAAGSDARMSGCELPVVINSGSGNQGISVSLPIIIYASSLNSP
                     KEELYRALALGNLIAIYLKRGIGKLSAYCGVSCAAAASAAGIAYLYKEPLNVIEATVS
                     NSLATISGMICDGAKPSCASKIAIAFDSAMLGYELAKGSNNFKGGDGIVKDDIDSTIR
                     SISKMAKDGMKETDNEILNIMLE"
     gene            complement(4693..5682)
                     /locus_tag="PLB33_08100"
     CDS             complement(4693..5682)
                     /locus_tag="PLB33_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOG63525.1"
                     /translation="MNKMLKKERVNAAIHKEEVDRPPFSIWYHVPHVDQDPVALAEEQ
                     IRLARNYDLDFIKMMPFGNYGAHDFGLSVDFFCTKTQPAFERKFAIDDIGEWKEIEPL
                     PGSFGSHGKQLLFARQMQKRLKGNDIPYVQTIFSPLTTAKKLAGPRIFNDLREKPEYL
                     HQALKAITETTVDFVKLNIEAGVAGFFFATQCATYDFMTESEYDEFGVKYDLEVINSY
                     KDLTYFNIAHVHGDNTMFNKLAQYPCNCINWHDRWTAPSMSEARKITDKCLLGGINEK
                     FLTKASPDEIYAHIHEGALNAGLRGLMITPGCVAELETPEVNFYAARLAVESL"
BASE COUNT         1779 a         1233 c          958 g         2037 t
ORIGIN      
        1 ctccatatag tatcaactat ttaatgctat taattctttt tgaataaatt tgtatataat
       61 cctgcagata ctaaaatcat atggttttag ttttatcttt ttttaaataa tccttaatat
      121 ctccccagaa aggccaaatc agcaatatta agtcaattgc aaataatgcc agagccaatg
      181 gtttaaacat aacagatgtt aaatctcctc ctgttattat catggttctt ctgaatgcca
      241 tttctgaagt ttttccaaga acaaatgtaa gaacaaaggg tgccaggggg aaattggatc
      301 tctgcatata aaaaccgatt attccgaata ccaatattaa taatgtatca aagaaaagat
      361 ttctgtaggt gaatgcacca agtacgcaaa gtgaacctat tatagggatt agtatagaat
      421 tgggcacttt tgttattttt gaataaatag gggcgccgta caatcctata agtaaaattg
      481 cgaatgttgc cataattatt ccaaaaaaca ttccatatgc aatttccggt gtttttgcaa
      541 ataactgagt tccgggagca agaccatgta ccagaagtgc gcccagaaat aatgcagacg
      601 ttccgttacc tggaattccc agtgtcaaaa gaggtattaa tgcgccggtt acttcggcat
      661 tgtttgctgc tgatacacat gccaaaccct gaggatttcc tttgccgaat gtttcaggat
      721 gaggatcgtc ttttttcgcc atgttgtagc ttataccggc tgctgcagtt gtagcggcac
      781 ctggaacaat acctataatt gtacctacga tggatgatct caaattattt ttaaacattg
      841 ctttatgttc ttttttagat aaaaatgggc ttccttttat ggcagaatct gtaatagcta
      901 ttgtagccga atgagttgca gccaaatcaa acatttgcga aacagcaaac aagcccagaa
      961 caacaggaag cagcggcaat gaatcaagta ttggttttat gccaaagctt aaacgagtca
     1021 atcctgttac tccatccata ccgattagag aacataaaaa accaaatgaa gcacttacaa
     1081 gaccttttaa aacaccttcc ttaaccattc ccaatattac aaccataccc atcattgtaa
     1141 ccagaaacag ttcccatggc ccgaacagta ccgccatttt agcaataatc ggagccagga
     1201 aaatcaaaga agcagcacca accattcctc caaagaatga actgaatgtt gtaactccaa
     1261 tagccttcaa cccctctccc cgctgagtca taggataacc gtcaattgtc ataaatacag
     1321 aatcagatgt accgggtgtg tttaccagta cagcagttat attgcctcca taggctcctg
     1381 ttacatagat agcccctaaa gtaataagag cagttgtggg atccagatta aaagtcaaag
     1441 ggataaatat tgcaagccct gtatcggcag acatgccggg cagtgcaccg agaatggcac
     1501 ctatgataca cccgattacc agccatagca tattctccgg tgttgctgct tgtatagcat
     1561 attgaaaaaa ttccatattt ttcaactcct taataaaaaa ttgtttcaaa attttaactt
     1621 aaaaatcgac ccattggaac tgccataccc agcagcaaat cgaaaaacaa gtacactccc
     1681 aatgtgaaca gcaatgatac aatcacgacc agtttcttat ttttctgtcc cagcgccagc
     1741 attgtaaaac ttagatatat aggtgttgca atataaaagc ttactatttc tattcctacc
     1801 acatataaaa agagtaaagc aagcattttt aatgcagtag tcataccggt tagattatct
     1861 tcatcagctt tctttgttgt catgaatttt gttctaataa caagcaatgt agctaaaatt
     1921 atggtagtaa tgcagattac tgtcgggaac atcctggatt ggggatcttt aattgaaaat
     1981 gagttaatta aaaatgatat cgatacaagg taaatgaaag cagctgacag atagttgctg
     2041 ttaaattttt tattataacc attttccatc tgaaatcacc tcctgttgat tccttttcta
     2101 aggagtcgta cgactcctta gaaatgttat ggataattac tttatcttta gttccatgga
     2161 tcctctgccc ataacttggc atatccttca gatatttgag taaatatagc ctttacttca
     2221 tctcccggca atgtatctat gggaagtttc atttcagcag cttttgcagc tacttcaggg
     2281 tcgtttgcag cagctataaa acattgttca aggtattttc ttataggttc aggtgtgcct
     2341 ttaggacacg aaacagctct catgttaacc tgtgtaacat catatcctgc ttccttgtat
     2401 gtgggaacat caggaaggaa tggagaacgg tcagaatctc ctgttgctaa tatagtaatt
     2461 gatttgtcct caataaagct cgtagcatca gaaacattca aacaaaaggc atcaagatgt
     2521 ttacccaaaa cagcagcaaa tattgcgcct tcgccgtcaa atgccacttc attgaaatct
     2581 actcctgctg ctctttcaat catttttatg gttagtcctt cttgccctat aacagaagta
     2641 acacctacag aaattttacc cgggttttct tttgcagctt gtacaagatc tcctagtgtt
     2701 ttataaggtg aaccggtaat aacgcctata gcacccggat ctgacatagt gttaaatatg
     2761 aaatctaatt tatcaactcc gtatgaaaca tcttcagata cagcagatat cagcattgca
     2821 ctaggtgcaa atcctgtaaa atatccatca ggctctgctt ctgcagaggc attccatccg
     2881 attcttccgc tggctccttc tttgaagctg attacaactc catttttcca gccttgcatt
     2941 tcctgcatct tatcacctag aattcttata taggcttcta agggtgaacc cgccgagaag
     3001 gcatagataa atgtaatttc tttattagga taatcttccg gcagtccttc cggtcttgga
     3061 acaactcctt ctttgttttc agatggtttt tcagcaggct cctccttttg gctgcagcct
     3121 gccattgcta aacttaacaa tataactaaa agcaaagcaa ttatggatat agtttttctc
     3181 ttaatcattt tctttctcct tataattatt ttttaatcta ttgtgatact tttatttgca
     3241 atatttatgc cataaatata aagcacttga aatatatttt tgttgaaaga gcagaatgta
     3301 tttattaaaa aactatctgc catataacca aattattaat aaaagctttt tcatcattga
     3361 aaagtttttc aaatttgaaa attttttctt ttttgaaaac aaattttgtt taatatggtt
     3421 atacttttta ctccaacatt atatttaata tttcattatc ggtctccttc attccatcct
     3481 ttgccatttt acttatgctt cttatagtac tgtctatatc atctttaact attccatcgc
     3541 ctcctttaaa attgttgctt ccttttgcaa gttcatatcc aagcatggca gaatcgaaag
     3601 ctattgcaat ttttgaagcg caggatggct tcgctccatc gcaaatcatg cctgatatgg
     3661 tagctaatga atttgaaaca gtagcttcaa taacattcag cggctccttg tataagtatg
     3721 ctattcctgc tgcactagcc gccgcagcac aggatactcc gcaatatgca gaaagctttc
     3781 ctattcctct ttttaagtat attgcaatta aatttcctaa tgctaatgcc ctgtataatt
     3841 cttcctttgg tgaatttaaa cttgacgcat agatgataat cggcagcgat acagatattc
     3901 cctggtttcc gcttccggag tttattacca caggcaattc gcatccgctc attcttgcat
     3961 ctgagcctgc tgcagcataa gctttcgcta tttcattaat atcgttaggg cttttactat
     4021 aaatagtttt gcctaccctt gcaccataat cattctctaa gccttcttta gcaatattca
     4081 gattgtattc tatctgttta tccagtatta attttacttc ttcaatattg acattgtttg
     4141 cataattata tatattttta aatctgaata tttcaatatc atcatcttct tcttccgaat
     4201 tgctgtcttt tttatatagc aaatcattat ttttgtgtaa ttcaataatg tttaaatgtc
     4261 catcctttaa tgttacggca gcacattcat cttcgtaaaa agattgaatt ttaatataaa
     4321 gatttgccac gttgggaacg agctttatct ggcacattcc ttcttcagca agctttttag
     4381 cttctgcgac cttgtttttc tcaattccgt taagcacttc aagttcttta tccgggtcac
     4441 cggctatggc accaagaaca acgcttattt ctataccttt taatccgtct gtacccggta
     4501 caacaacgct tttggcattc tttacaatat tcccgctgca gctgcatatt attctttcag
     4561 gaatgtgccc taaaacctgc gtagctttag ctgctgcata tgcaagtgct atgggttctg
     4621 tacaaccaaa tgcaggtatt aactcttctt ttaaaatttc agtatatctg ttcataataa
     4681 tcccctattt tcttataagc tttcaactgc taatcttgca gcatagaaat ttacttccgg
     4741 tgtttccagt tctgcaacgc agccgggtgt aatcatcaat cccctaaggc ctgcattcaa
     4801 tgcgccttca tgaatatggg catatatttc atcagggctt gcttttgtta agaatttttc
     4861 gtttattccg cccagcaggc atttatcagt aatttttctt gcttcactca tgcttggggc
     4921 agtccacctg tcatgccagt ttatacagtt acagggatat tgggcaagct tgttaaacat
     4981 tgtgttgtct ccatgaacgt gggcaatatt gaaataagtt aaatccttat atgaatttat
     5041 tacttccaga tcatatttta ctccaaactc atcatattcg gattccgtca taaaatcata
     5101 tgttgcacat tgagtagcaa agaaaaatcc ggcaactccg gcttcaatat ttaattttac
     5161 aaaatcaaca gtagtttctg ttatagcttt taatgcttga tgcagatact ccggtttttc
     5221 tctcaaatca ttaaaaattc taggaccagc caattttttt gctgtagtaa ggggactgaa
     5281 tattgtttga acatatggta tgtcatttcc cttcaatctt ttttgcattt gtctcgcaaa
     5341 cagcagctgt ttaccgtgtg aaccgaaaga tcccggcaag ggttcaattt ccttccattc
     5401 gcctatatca tcaattgcaa attttctttc gaaggccggt tgggttttag tacaaaaaaa
     5461 gtcaacgctt agtccgaaat catgtgcccc gtaatttcca aaaggcatca tcttaataaa
     5521 atccaaatca taatttcttg ccagtcttat ttgttcttct gcaagagcta caggatcctg
     5581 atccacatgc ggtacgtgat accagatgga aaacgggggc ctgtcaacct cttccttatg
     5641 aatagctgca tttactcttt ccttttttaa catcttattc attttatact ccttttaaat
     5701 tattaaagta tattttgttt gattatatat attgcaaatt tcatgccatt aaacatcaaa
     5761 tctttttgct taggtttaca ttcattacac actttatatt tgtggataca ttataactca
     5821 tttaatcaca tctgagaaat tattttttga aaaaattttt attaatgata gcattaactt
     5881 gtataaatgt caaaacctgt taattattat gccttgatac catgaaacac aggtttaccg
     5941 cacacggctc cttattttta ttacattgcc cgtgtgcgaa aagaaacagc gacctctttt
     6001 cagaaat
//
LOCUS       DAOSHP010000095         5883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_11954_length_5883_cov_9.331846, whole genome shotgun sequence.
ACCESSION   DAOSHP010000095 DAOSHP010000000
VERSION     DAOSHP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11954_length_5883_cov_9.331846"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLB33_08105"
     CDS             <1..245
                     /locus_tag="PLB33_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose dehydrogenase"
                     /protein_id="HOG63526.1"
                     /translation="FIDFVRRGSTPARGVLAYTKVRTECKLNDYSIIEPNYNFGTQSA
                     YYVSLGANLTQSRLYLGVYGSSNVTDFNQGTVFEIV"
     gene            complement(371..2806)
                     /locus_tag="PLB33_08110"
     CDS             complement(371..2806)
                     /locus_tag="PLB33_08110"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG63527.1"
                     /translation="MKKGIILLLIITLILSLFQTAFAQIYDISNHWASKEINYLVERE
                     VLSGYPDGTFKPDNPITKAEFYKVINKLLNFEERADITYSDVNETDWYYEDVGKALAA
                     GYISPATVLLPNEYITRQEVARILGFVYNLDDESESASVFSDSESILPEIRGYIGVLF
                     ENNLLHGYPDGTFKPSANITRAEVVKLIYNVLEKLNNLPIKVSANESEKVSDSSHRDT
                     SVKRYTLTEGTNEITTHVKIRTATVIGNVTIEYTGSADLKLPAGLTVDGDLTVIAPKA
                     TVNNYARVTGNIIIRDVASTTWNEYADGNILIVEDENGLVLHVVGNVRGITVSESTDG
                     NITIALSRDVNTDAIEFIAIKGPVTLITQKLIQATIKEGTTIIVKSSIDGKEIEFTGT
                     GEEVEILDGYPIVPVTINYLDEKTKEVIASVIEYSIDNGENWIAGPGSELDLIPGKDV
                     IFRIAEMPSRELQTLIVPERPEGISFSYADSVSDYLMGLPAGATNIEARILAGERVYQ
                     DWTDITVDSDGKASLIHGGGDLIQVRTKAVTGSAFAGDIVQHYSKKSISELDIGDLVV
                     DNSWQWEYKTGSGYSGTGELKPVTWIVAAKEHYGMDSGVTLLSKDLIGQFTFDNRTDV
                     HHNGVNLWGPSNHIRPWLNSVGIYEENGFYNAFSIEFMNSVLMTDVPNEGYWEGKYTT
                     QDKVFIPSITELGAPEVALEYYDEELDETFEIGITYVIGTVYPYFENASNSDRIAKLG
                     ASNFSYWTRSPEDWLKDIVHCILNDGSRGIYWGENLQAKDNNGVRPAVNIAPDTLVSG
                     IPNENGAYEIFYE"
     gene            2989..3609
                     /locus_tag="PLB33_08115"
     CDS             2989..3609
                     /locus_tag="PLB33_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG63528.1"
                     /translation="MEKKNINKRKLKAIETKNKIYEAARKLSLEHGIEKVSVDTIVEA
                     AGVSKGAFYVHFESKDALTAELVDEYTNKADIDYKAFLNSLPDNMPVLDILVLLAEEI
                     CDFIETNIGLENMRVLYKAHLTKTINTSSAMSYNRELYNLFTEVLEKGLMRGEIRQDI
                     PVNNMAKHLVLAIRGITYEWCIRYPDIDLKEYVLDHFKILLYGLES"
     gene            3768..4688
                     /locus_tag="PLB33_08120"
     CDS             3768..4688
                     /locus_tag="PLB33_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG63529.1"
                     /translation="MKTKLMADSTCDLSQEILNRYNICLAPLVVSIEGKEYRDRIDIS
                     NDEFYKNLMTFKPYPTTSMPSPESYIACIKHAIEQGYEKILCICMSSGTSGSYQSALL
                     AKELFYEQNPKSNIQFEIIDSVSMSHGSGWLIMKCAMFIENGYTFEELIEYCEIIKYR
                     IKHFLSVDDLDSLIRSGRLSNTSGVIGKLLNVKPIMSMKNKKGTVVAKKRGIKQVYNY
                     YIQEFINRVDYEQTDFVIIGYTSDINKAENLKALLLKANYKGDIYIMQMGVAVGTHVG
                     LGGLSMFFVEKPHEDFINHMKEKIISHIHS"
     gene            complement(4752..5855)
                     /locus_tag="PLB33_08125"
     CDS             complement(4752..5855)
                     /locus_tag="PLB33_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HOG63530.1"
                     /translation="MNFLQLDTPALLIDKDIMLNNLKHMQDYANEKKVALRPHTKTHK
                     MSKLALLQEELGAKGITVAKVGEAEVMAANGLRDIFIANEIVGETKLSRVKKLAETID
                     ISFGLDSIAQADMVEKVFEGSKRANVLIEIEVGENRSGVIEEDDYIELLNYLKQCSNI
                     NLKGVFSHDGHSYSAENIEACKEIHLEAQRRTLRFANIANEMGFKLETVSIGSTPSLM
                     HNFPILEGVTEIRPGTYIFMDASQANAYGNVDRNAATILTTVISLPTPERVITDVGAK
                     GITMQARSKGFTATKGLGLIKGWPEVSIYAVYDEHAIIYNKKFHDSMKVGDKVEIIPN
                     HICPVVNLYETAYIIENGRVVDEIPVDCRGKLK"
BASE COUNT         1836 a         1147 c          933 g         1967 t
ORIGIN      
        1 tttttataga ttttgtcagg agaggaagca cacctgcaag aggagtattg gcttacacaa
       61 aagtgcggac ggaatgcaaa ctgaatgatt acagcattat tgaacccaat tataattttg
      121 gaactcaatc agcttactat gtaagtttgg gagcaaacct cactcaatca agactatatt
      181 taggtgtata cggctcatcc aatgtaactg attttaatca gggaactgtt tttgaaattg
      241 tataacctaa tcatgaattg attaaatgtt aatcagtatt catataataa tataaggcca
      301 gtatagataa tcaaataacc aactggcctt ttttctattt ataatcttaa tttatcttgc
      361 tgcagtcaac ctattcatag aaaatctcgt aagccccatt ttcattcggg atacccgata
      421 ccagtgtatc aggtgctata tttacagcag gtcgaacacc attattatct tttgcttgta
      481 gattttcacc ccaataaatt ccacgacttc cgtcatttaa gatgcaatgc actatatctt
      541 tcagccaatc ttccggagac cgggtccagt agctaaaatt gcttgctcct aatttagcaa
      601 ttctatctga attggatgca ttttcaaaat atggataaac tgtacctatt acgtaagtaa
      661 ttcctatttc aaaagtttca tcaagttctt catcataata ttccaaagcc acctctggag
      721 cacccaattc tgtaattgat ggaataaaca ctttatcttg tgttgtgtat ttgccttccc
      781 aataaccttc atttggtaca tcagtcatta ataccgaatt cataaattct attgaaaaag
      841 cattgtaaaa tccattttcc tcatatatcc ccacactatt cagccatggg cggatatggt
      901 tgctgggtcc ccaaagatta actccgttat gatgtacatc agtcctattg tcaaaggtaa
      961 attgacctat taaatcttta gacaacaagg tcactccgga atccatgccg taatgttctt
     1021 ttgctgctac aatccaagta accggtttta gttcaccggt tcccgaatat ccgctgccgg
     1081 ttttatactc ccactgccaa gaattatcta caaccaaatc tccaatatct aattcactga
     1141 tacttttttt ggaataatgc tgaacaatat ctccggcaaa ggcggacccg gttacagcct
     1201 ttgtcctgac ttgaattaaa tctcccccac cgtgaattaa acttgccttt ccatcagagt
     1261 caactgttat atcagtccaa tcctgataaa ccctttcgcc ggctaaaatt cttgcttcta
     1321 tattagttgc gcctgcaggc aaccccatta aataatcact gacactatca gcataggaaa
     1381 atgatatacc ttctggccgt tccggtacta taagtgtttg taattccctc gacggcatct
     1441 cagcaattct aaaaataaca tcctttccgg gtatcaaatc caattcactg ccgggtccgg
     1501 ctatccagtt ttctccatta tcaatgctat actcaataac tgaagctata acttccttag
     1561 tcttttcatc taaataatta attgtaaccg gaactatggg ataaccgtct aatatttcaa
     1621 cttcctcgcc ggtacctgta aattcaattt cttttccatc aatactggat ttgacaatga
     1681 ttgttgttcc ttccttaata gttgcttgta taagcttttg cgttattaat gtaacaggtc
     1741 ccttgattgc tataaattct atagcatccg tattgacatc tctgcttaaa gcaattgtta
     1801 tatttccatc tgttgattcg cttactgtaa tacccctgac atttccaact acatgtaata
     1861 ctaagccatt ttcatcttca acaattaata tattgccatc tgcatattca ttccaagttg
     1921 tgcttgctac atcccggatt attatattcc cggttactct tgcatagttg tttacagtag
     1981 cctttggtgc tattacggtt aaatccccat ctacagtcaa tccggcaggt aattttaaat
     2041 ctgcactgcc ggtgtattca attgtcacgt ttccaatcac tgtagctgtt cttattttaa
     2101 cgtgtgtagt tatttcattt gttccttctg ttaatgtata tctttttact gaagtatccc
     2161 tgtgagatga atcagatacc ttctctgatt cattggcact tacctttatc ggtaaattat
     2221 tcagcttttc aagaacattg taaatcagct taactacttc agctctggta atatttgctg
     2281 aaggcttaaa tgttccatcc ggatatccat gtaatagatt gttttcgaat aatacaccaa
     2341 tataacctct tatttcaggc aatattgact cagaatccga aaatacgctt gcagattctg
     2401 actcatcatc aagattataa acaaatccta agattcttgc tacctcttgc cttgttatat
     2461 attcatttgg caacaatact gttgcagggc tgatgtatcc tgctgccaat gcctttccca
     2521 catcttcata ataccagtct gtttcattta cgtctgaata tgtaatatct gctctttctt
     2581 caaaatttaa taatttatta attactttgt aaaactccgc ctttgttatt ggattatccg
     2641 gtttaaaagt tccatccggg tatccgctta atacctctct ctctaccaaa taattaattt
     2701 ctttgcttgc ccaatggtta cttatatcat atatttgagc aaatgctgtt tggaataaag
     2761 atagaataag cgtaataatc agtagcaata taataccttt tttcataaat cttcctccta
     2821 ttatgacttg tctaataagt gaaacttaag agtttctttt ttaatatcca tctatacaaa
     2881 aaatggaata tatgttataa tttaaaaaat gcagtataat gactgcagtc acataccgta
     2941 gtcattatac aatatttgtt ccaatcagtc aatcacaagg agggaagaat ggagaaaaaa
     3001 aatataaaca aaagaaaatt gaaagcaatt gaaacaaaaa ataaaattta tgaagccgcc
     3061 cgaaaattat ctttggagca tggaatcgaa aaagtaagtg ttgataccat tgtagaggcg
     3121 gcaggagtat ccaaaggtgc attctatgta cactttgaat caaaagatgc gcttacagct
     3181 gaacttgtag atgaatatac aaacaaagca gacattgatt ataaagcttt tctaaattct
     3241 cttcctgata atatgcctgt ccttgatatc cttgttctat tggcagaaga aatttgcgat
     3301 tttattgaaa caaatatcgg attagaaaat atgagagtgc tttataaagc tcacttgaca
     3361 aagactataa acacatcatc tgcaatgagt tataacaggg agctgtataa tttgttcact
     3421 gaagtattgg aaaaagggct catgcggggt gagataagac aagacattcc tgttaataat
     3481 atggcgaaac acttggtctt agctatcaga ggcattacat atgaatggtg tatccgatat
     3541 cccgatattg atttgaagga gtatgttctg gaccacttta aaatactgct ttacgggtta
     3601 gaaagttaaa aatgcaatat atctcttgta tatgcttacc aatttaatat acgagctgct
     3661 aaaaattgta tttcaaagaa ttacgaaaaa tgttgaatat tgtcgaatag gatggtatga
     3721 tagtgcaaat atacaagcac taaaatacta taagaacggg agcttatatg aagacaaaat
     3781 taatggcaga ttcaacgtgt gatttatctc aagaaatttt aaatcgttac aatatatgtt
     3841 tagctccact ggttgtttca atagaaggaa aggaatacag agaccgaata gatatttcaa
     3901 acgatgaatt ttataagaac ctgatgactt tcaaaccata tccgaccact tcaatgccaa
     3961 gtcctgaatc atatattgcc tgtatcaaac atgccataga gcaaggatat gagaaaatac
     4021 tctgtatttg catgtcaagc ggaacaagcg ggtcttatca aagcgcttta ctggcaaaag
     4081 aattatttta cgaacaaaat ccaaagtcta atattcaatt cgaaataata gactcggtta
     4141 gcatgagtca tggtagcgga tggcttatta tgaaatgcgc catgtttatt gaaaacggat
     4201 atacgtttga agagcttatt gaatattgtg aaataataaa atacagaatc aaacactttt
     4261 tatccgtaga tgatttggat agtctgatac gcagcggaag gttatcaaat acaagcggcg
     4321 ttataggcaa attgctaaat gtcaagccga ttatgtcaat gaaaaataaa aaaggcactg
     4381 tagtggcaaa aaaacgagga attaaacaag tttataatta ttatattcaa gaatttataa
     4441 accgggtaga ttatgaacaa actgattttg ttatcatcgg atatacatcc gacattaata
     4501 aagcagaaaa tttgaaggcc ctgctcctta aagcaaacta taagggtgac atatacatta
     4561 tgcaaatggg agtagctgtt ggtactcacg taggtttagg cggtctttcc atgttctttg
     4621 tcgaaaaacc tcatgaagac ttcataaatc acatgaaaga aaaaataata agtcatattc
     4681 atagttagtg ataaagggga tgttaagcac atccccttta ttttaaattc aactatttac
     4741 gttcacttat cctatttaag ctttcctctg caatcaacgg gaatctcatc caccacacgg
     4801 ccattctcaa taatataggc tgtttcatac aagtttacca caggacatat atgattggga
     4861 attatctcta ccttatctcc gactttcatt gaatcgtgaa atttcttgtt atatataatt
     4921 gcatgttcat cgtatacagc ataaatgctt acttccggcc atccttttat aagacccaag
     4981 ccttttgttg cagtgaatcc cttacttctt gcctgcattg taattccctt ggcaccgaca
     5041 tccgtaataa ccctctcagg tgtaggtaga cttattacag tagttaagat tgtagctgca
     5101 ttcctgtcca catttccgta tgcattagct tgagaagcat ccataaaaat atatgtgccc
     5161 ggtctgattt cagtgactcc ttccaatata ggaaaattgt gcattaaaga aggagttgag
     5221 ccgatactga ctgtttctaa tttaaatccc atttcatttg caatatttgc aaatcgcagg
     5281 gttcttcttt gagcttccaa atgtatttct ttgcatgctt caatattttc ggctgaataa
     5341 gaatgaccat cgtgagaaaa aactcccttt aaatttatgt tgctgcattg tttcaaataa
     5401 tttagaagct ctatgtaatc atcttcttca ataactccgg agcggttttc tcccacttca
     5461 atttcaatta atacatttgc tcttttgctg ccctcgaata ctttctcgac catatctgcc
     5521 tgggctatgc tatcaagtcc aaatgaaata tctattgttt cagccagttt tttaacacgt
     5581 gaaagcttcg tttcgcctac aatctcatta gcaataaaaa tatcccttag tccgtttgca
     5641 gccattactt ccgcttcacc aaccttagcc accgttatgc cttttgcccc caattcttcc
     5701 tgcaataaag ctaatttaga cattttatgt gtcttggtgt gaggccttaa tgctactttt
     5761 ttttcatttg cgtaatcctg catatgtttt aaattattca acataatatc cttatcgatt
     5821 aacaaagccg gtgtatctaa ttgtagaaag ttcatgtttt ttctccttac tttaactgat
     5881 att
//
LOCUS       DAOSHP010000096         5844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12068_length_5844_cov_11.235965, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000096 DAOSHP010000000
VERSION     DAOSHP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12068_length_5844_cov_11.235965"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1587
                     /locus_tag="PLB33_08130"
     CDS             337..1587
                     /locus_tag="PLB33_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63531.1"
                     /translation="MKFNRKSVIISIFVLGVLIFATSAFADIMLGSGYNSLKDSVKAT
                     AEKLTHEADNFSVDYMVSIKVDGKTVDESTENGKYDLINQAQESNSTYFEKGQRKERY
                     SYNDPNKRIYKNFEDGSYNVVEKRKYSNERPFIENPFEEEQAKDAEKIMDAFVGSLKD
                     VIQIEESGGNKMYMGNIGDSEIPPLINAISSFALKYSILNEYTAERLNIPSPKSNVYV
                     KDASGKAIENEDGILESGIFTASMSAEDSNGTEHIYTLEFTIDIKDINNTIVAAPDLD
                     GQKVTYSKEGFEFDEKYIGKYKNNIVKVEDNSFVKIGERIIEITSVEDGNVKGRYYEV
                     YSEEYEPDIVRNFEFFSNHDDSEFYTILNYTNNNGEKKQGVMHRTNLQDLYVSFNITF
                     DEDGSGYSAANIDDFNSEFVRVFE"
     gene            1647..2372
                     /locus_tag="PLB33_08135"
     CDS             1647..2372
                     /locus_tag="PLB33_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOG63532.1"
                     /translation="MDKAIVIENLSKTYKNSRGIQDINLEIQRGEIFGFLGPNGSGKS
                     TTMKVMVGLMKADKGDVRINGYSISKDYEQAMKRVGCIIENVAPFPYLTAYENMKLCG
                     RFHENVDEGRIDECLDITGLLKYKNEKIKNYSLGMKQRIGIAMAILSNPEVLILDEPL
                     NGLDVKAMVEMRKLFLNLSNQHKKTFFISSHLIHDIELTCSKVGILYDGKLLGVDSTQ
                     NILKNYSSLENYYLSEVDLNGAS"
     gene            2359..3102
                     /locus_tag="PLB33_08140"
     CDS             2359..3102
                     /locus_tag="PLB33_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63533.1"
                     /translation="MEPLKAVYINELFKISKKKKIKAALIFSILSVIVAAIAVYFLNN
                     FAGIRITGSSDFSIMVLTVLSYTLFPLFTAFICIDMFAGEFADDTIKFTLTGPAPRFK
                     VFSGKILAIAGFIIVNLIFVMILSLIISIFINGNMTNVLKILLAYIMEFFPQLIFALT
                     VVFISNITRGTTSAFMLSILIFLLFNGLGFIFPSYKSLFFTSAFDWYTLILGGYINFS
                     KILRVFLILLGYGIMLYTASYYLFEKKEV"
     gene            3110..4429
                     /locus_tag="PLB33_08145"
     CDS             3110..4429
                     /locus_tag="PLB33_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOG63534.1"
                     /translation="MNLKQRFLKQSILTLIITIIITGCVGFVYDYFYNLLNKSSVYVG
                     LGKTATVVIENDNIIYNDEEFSSLQIKEMMMNISVESNYYEHDNTKFILDTETFTTVS
                     GDEFMIITLNAVTDASDYYRSLMLLVFIIFLIVFIVTNIVVQKQNMINIINPIINLTK
                     ETERLRTGELETAIIEQGYGEVRELGSAVEQLRLQLKNSIYYQQKFDENRKFLISSIS
                     HDLKTPVTSIRGYLDGVLDGVADTDEKKKYYLSKAVDKTKLINNMIEDLLLYSKLDLN
                     QMPFEKDRINIVKYIESCLEDNSAEFERENKGIILDNKLSDEYLVNIDREKFKRVIQN
                     IVDNAKRNIKKEFGKLTIILRETNSSVIMEFKDNGKGISKDDLPYVFDKFYRADTARA
                     VEGSSGLGLAIAKQIVEGLGGRIWAISEIDEGTSIIISLKKAIERKW"
     gene            4431..5114
                     /locus_tag="PLB33_08150"
     CDS             4431..5114
                     /locus_tag="PLB33_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG63535.1"
                     /translation="MKRILIIEDDKSISELQKDYLEMSGYEVLCVFDGETGLEHIIND
                     DFDMVILDLMLPQKDGFDILKEISDSKQIPVLIVSAKSDEMYKIKGLNLGADDYITKP
                     FSMGELVARVNSHLKTYERFNNNSNKKQISAGAISIDKEDRRVYLENNEVFLTQKEFD
                     LLLFLVENPNRVYSKDELYEKVWGYDALSDTTTITVHIARIREKIEIYPNGHQYIETV
                     WGAGYRFKI"
     gene            5204..5632
                     /locus_tag="PLB33_08155"
     CDS             5204..5632
                     /locus_tag="PLB33_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63536.1"
                     /translation="MDIEVFVKRVYTVSFRLTGQEQVAEEIASQAIIHTFKEMNEDYK
                     EKVTENMFHLTIMELVKIFLNNPNSQCNDNLKGIKRALLELKPINRAVVIWKDVLGYK
                     IHDNIPVSDYTYEELYEELVCGRKELKEYKILRASGEQFS"
BASE COUNT         2199 a          719 c         1095 g         1831 t
ORIGIN      
        1 gctaaatcta aaaaaaagac tttatttata atagagcctg atacattaaa tattaaatcg
       61 ccattaagaa tttgcagata attgctgaaa tctaaagctt ttattctttc aagtttatta
      121 tattaagatt atataatgac ggaaagaaat atattagaga aaaagttggg tagaagatag
      181 attttctaat tttcataaat ctttactaag tctgaagatt taataaattg ttaataatta
      241 gtttacagat attcaagact aacaacgtag tatgtatata ttacataaac gtttatgatt
      301 ttgtggtaat taaaatcaaa ttaggaggcg gggaaaatga aatttaacag aaaatcagtt
      361 ataatcagca tttttgttct tggtgtattg atttttgcaa catcagcatt tgcagatatt
      421 atgcttggtt caggatataa cagcttaaaa gattctgtaa aggcaacggc tgaaaaattg
      481 acacatgagg cagataactt cagtgtagat tatatggtat ctataaaagt tgacggcaag
      541 acagttgatg aatctactga aaatggaaag tatgacttga taaatcaagc acaggaatca
      601 aacagtacat attttgaaaa aggtcaaaga aaagaaaggt attcctacaa tgacccaaat
      661 aaaagaatct acaaaaattt tgaagacggc tcatacaatg ttgtagaaaa acgaaaatat
      721 agtaatgaaa gaccatttat tgaaaatcca tttgaagaag agcaagccaa ggatgcagaa
      781 aaaattatgg atgcatttgt aggaagctta aaagatgtta ttcaaattga agaatcaggt
      841 ggaaataaaa tgtatatggg caatatcgga gattctgaaa ttcctccact tattaatgct
      901 atctcatcct ttgcattaaa gtattcaata cttaatgaat atacagcgga gagactaaat
      961 attccttcgc ctaaatcaaa tgtatacgtt aaagacgcat caggcaaggc aattgaaaat
     1021 gaagatggaa ttttagaaag cggaatcttt actgccagca tgtctgcgga agacagcaac
     1081 ggcactgaac atatttatac tttagaattt acaattgata ttaaggatat taataataca
     1141 atagttgctg ctccggattt ggatggacaa aaggttactt acagcaagga aggctttgaa
     1201 tttgatgaga aatatatcgg aaaatataaa aataatattg taaaggtaga ggacaattcc
     1261 tttgtaaaaa taggcgaaag aattatagaa ataacttctg ttgaagatgg caatgtaaag
     1321 ggcagatatt atgaggtata tagtgaagag tatgaaccgg atattgtaag aaactttgaa
     1381 ttcttttcaa atcatgacga ttcagaattt tacacaatat taaactatac aaacaacaat
     1441 ggcgaaaaga aacagggagt tatgcaccgt acaaatttac aagacctata tgtatcattt
     1501 aatattacat ttgatgaaga tggcagtgga tattcagctg ctaatattga tgacttcaat
     1561 agcgaatttg taagagtatt tgaatagcaa tgcaggcgca aggcttgggt ctttgcgcct
     1621 caaagcaatt ttttaaggag gatatgttgg ataaagctat tgtaattgaa aatctttcaa
     1681 aaacctataa aaacagccgc ggaatacaag acatcaattt ggaaattcaa cgtggtgaaa
     1741 tattcggatt tttaggtcct aatggttcag ggaagagcac tacaatgaaa gtcatggtcg
     1801 gcctcatgaa agcagacaaa ggcgatgtca ggataaatgg ttacagtatt tccaaggatt
     1861 atgaacaggc aatgaaaagg gttggttgta ttatagagaa tgtagccccc tttccttatt
     1921 tgaccgcata tgaaaatatg aaattgtgcg ggagatttca tgaaaatgtt gatgaaggac
     1981 gaattgatga atgcttggat attacaggac ttcttaaata taaaaacgag aagataaaga
     2041 actattcctt aggtatgaag cagagaatag gtatagccat ggccatttta tcaaatcctg
     2101 aggttttgat attagacgag cctctaaatg gtttagatgt taaagctatg gtagaaatga
     2161 gaaaattatt tcttaacctg tcaaatcagc acaaaaaaac attttttata tccagtcatt
     2221 taatacatga tatagaactt acgtgcagca aagtaggcat tttatatgat ggtaaattat
     2281 taggagtgga cagcacacaa aacattttaa aaaattattc ttccttggaa aactattatt
     2341 taagtgaggt tgatctaaat ggagcctctt aaagcagtgt acataaatga attattcaaa
     2401 atatcaaaaa agaagaaaat taaagcagcc ttgatatttt caattctatc ggtcatagtt
     2461 gcggccattg cagtatattt tttaaataac tttgcaggga tacgtatcac aggaagctct
     2521 gacttttcca tcatggtttt aactgtatta agctatacac ttttccctct ttttactgct
     2581 tttatatgta tagacatgtt tgccggagaa tttgcagatg atactattaa atttaccttg
     2641 acagggcctg cgccgagatt taaagtattc tcgggaaaaa ttcttgcaat tgccggcttt
     2701 ataatagtaa atcttatttt tgtaatgatt ttatccttaa taatctctat atttataaat
     2761 ggtaatatga caaatgtttt aaaaatattg ttagcatata ttatggaatt tttcccgcag
     2821 cttatttttg cattaacagt tgttttcatt tcaaatatta caaggggaac aacaagcgcc
     2881 tttatgcttt ctatacttat atttttattg tttaatggat tgggatttat ttttccatct
     2941 tacaaaagtt tatttttcac atctgctttt gattggtaca cattgatttt aggaggctat
     3001 ataaatttta gcaaaatact gagagtattt ctaattttgc tcggttatgg tataatgtta
     3061 tatacagcaa gctactatct ttttgaaaaa aaggaagttt aggtatacta tgaatttaaa
     3121 acaaaggttt ttaaaacaaa gcattctaac acttataatc acaataatta ttaccggatg
     3181 tgtcggcttt gtctatgatt atttttacaa tttattaaat aaatcttctg tttatgttgg
     3241 tttgggaaaa acagccactg tcgtcattga aaatgataat atcatatata atgacgaaga
     3301 attcagcagc ctgcaaataa aagaaatgat gatgaatata agtgttgaaa gtaattatta
     3361 tgaacacgat aatactaaat ttatcttgga tacagaaact tttacaacag ttagcggcga
     3421 tgaatttatg attataactc tcaatgcagt cacagatgca agtgattact acaggtcact
     3481 tatgctatta gtttttataa tatttttaat agtttttatt gtaacaaaca tagttgtaca
     3541 aaagcaaaac atgataaata tcataaatcc tattataaat ttgacaaagg aaacggaaag
     3601 gttgagaacc ggggagcttg aaacagcaat tatagagcaa ggttatggtg aagtcagaga
     3661 gttaggaagt gctgtagagc aattgcgcct tcagttaaaa aactctatat attatcagca
     3721 gaagtttgat gaaaatagaa aatttttaat atcaagcata tcacatgacc ttaaaactcc
     3781 tgttacatcc ataagaggat atttagacgg agtattagac ggtgttgccg atactgatga
     3841 aaagaagaaa tattatttat caaaagccgt tgacaaaaca aagctcataa acaacatgat
     3901 tgaggattta ttgctgtatt ccaagcttga tttaaatcaa atgccattcg aaaaagatag
     3961 gattaacatt gtaaaataca tagaaagctg ccttgaggat aattcagcgg aatttgaacg
     4021 tgaaaacaag ggaattattt tagataataa gctttcagat gaatatctcg ttaatataga
     4081 tagggaaaaa tttaaacgtg tgatacaaaa tatagttgat aatgccaaaa gaaatataaa
     4141 aaaagaattt ggaaaactta caattatact aagagaaaca aattcttcag taataatgga
     4201 gtttaaggat aacggcaaag gaatcagtaa ggatgatttg ccttatgttt ttgataagtt
     4261 ttacagggca gacacagcaa gagcagttga aggttcaagt ggtttgggtc tggcaattgc
     4321 aaaacaaata gtagaaggtt taggcggacg aatttgggca atttctgaaa ttgatgaagg
     4381 aacatccatt attatttcat taaaaaaggc tattgagagg aagtggtaaa ttgaaaagga
     4441 tattaattat tgaagatgat aaaagtattt cagagctgca aaaagattat ttagaaatga
     4501 gcggttatga ggtcctatgt gtttttgacg gcgaaaccgg gcttgagcat ataataaatg
     4561 atgacttcga tatggttatt ttagatttga tgcttcctca aaaggatggc tttgatatat
     4621 taaaagaaat atccgattca aaacaaatac cggtgcttat tgtgtcagca aaatctgatg
     4681 aaatgtataa aattaaagga ttaaacttag gagcagatga ttacataact aaacccttta
     4741 gcatgggtga attagttgca cgggtaaaca gtcacttaaa aacatatgaa aggtttaata
     4801 acaattcaaa taagaaacaa ataagtgcag gagccatatc tatcgataaa gaagaccgta
     4861 gagtttattt agaaaacaat gaagtttttt tgactcaaaa ggaatttgat ttgcttttat
     4921 ttttagtaga aaatccaaat agggtttata gcaaggacga gttatacgaa aaggtttggg
     4981 gttatgatgc tttgtcagat acaacaacaa taactgttca tatagcaaga attagagaaa
     5041 aaatcgaaat atatcctaat ggacatcaat atattgaaac tgtatgggga gcaggataca
     5101 gatttaaaat ttagtataaa taacatatga ttttatttgc cttttaattt atagtctagc
     5161 aattttttta atatgaggat ttgttaatat aggtggggga gttatggata ttgaagtatt
     5221 tgttaaaaga gtatatacag tttcattcag gcttaccggc caagagcagg ttgcagaaga
     5281 aatagcttct caggcaataa tacatacttt taaagaaatg aatgaagatt ataaagaaaa
     5341 agtaacagaa aacatgtttc acttgacaat tatggagttg gtaaagatat tcttaaacaa
     5401 tccgaattcg caatgcaacg ataacttaaa aggaattaaa agggctctat tagaactcaa
     5461 acctataaac agagcagtag ttatttggaa ggatgtatta ggctataaaa tacatgataa
     5521 cattcctgta tctgattaca cttatgaaga gttatatgaa gaacttgttt gcggtagaaa
     5581 agagctgaag gaatataaaa ttcttagggc ttctggagag caatttagtt gaagaggaaa
     5641 aatgcgaatt gacaatctaa agtttaatgt ttctgaaata atccttgaca aactcattct
     5701 acaatcgtag aatgagttta ataatataat ttaaatttat tttgagagga tgtgaggtgg
     5761 tggaaatgga tactattaaa agaattcctg acagtgaatt ggaaataatg atgataattt
     5821 gggaagcaaa tgagccggta tcat
//
LOCUS       DAOSHP010000097         5793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12213_length_5793_cov_11.347159, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000097 DAOSHP010000000
VERSION     DAOSHP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12213_length_5793_cov_11.347159"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLB33_08160"
     CDS             complement(<1..792)
                     /locus_tag="PLB33_08160"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOG63537.1"
                     /translation="MFGYVTINKMELKFKEYYSYKGYYCGLCKCLKTMYGNKSRLTLN
                     YDMTFLILLLSSLYEPENKIYNERCIVHPKNKQLIIQNEITEYAASLNVLLSYYNMLD
                     NWVDDRDYKSLAAAKILQSQLQKSVSNLNDITKTIKLRLNNISKLEKEDTEDIDAVSN
                     EFGHLMEEIFLYKKDHWEQSLRKIGFYLGKYIYFIDAYEDMKKDLENKSYNPFNKLNI
                     ENKEEYANNLLMLNLSFLSDEIEKLPLVRDKGIIDNIIYSGMLNKF"
     gene            1135..1782
                     /locus_tag="PLB33_08165"
     CDS             1135..1782
                     /locus_tag="PLB33_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOG63538.1"
                     /translation="MNTDKKEITSALTHLGGAIFGIIALILLLSAAIKEDSLIALASF
                     LIFGLSMIFLYCTSCIYHFIDSSKKKAKVIMRKLDHIMIFVLISGTYTPVCLLILTKN
                     IGYKLLALVWSITIIGALIKIFWIDAPRWVSAGLYLGMGWMSVLVFMPLVRSMETGGI
                     IWLCLGGLLYTAGGIIYGLKKPAINKQWFGFHELFHIFVLAGSFCHFMMMYFYVA"
     gene            complement(1825..2037)
                     /locus_tag="PLB33_08170"
     CDS             complement(1825..2037)
                     /locus_tag="PLB33_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63539.1"
                     /translation="MIKIKLSTVDSDVENIDLDYKDITIDEIITELKIDKQHIGAVLV
                     NGIPKKFSDKVEDNSEVFFLPILSGG"
     gene            complement(2009..>2967)
                     /locus_tag="PLB33_08175"
     CDS             complement(2009..>2967)
                     /locus_tag="PLB33_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOG63540.1"
                     /translation="NDLTGLPVTNFKGAKFEHAEEISGQSLYELIKERGGEGKTAHAC
                     MPGCIIQCSNVVPDEEGKTIVSPLEYETIGLMGSNLGIGDLDTIAKLNAVCNDLGVDT
                     IETGAVMGMAMEAGIVEFGDNEAALNLLDEIKTGTELGKLLGEGTLIAGKALGVKNIP
                     VINGQAMPAYDPRAIKAMGVTFATSPMGADHTYGPVVKAEDAVEASKAAQIMMAKIDC
                     LGVCMFLRGAVAKHMDLFVDLVNAVHGWDLDMDWLEDIALEAIRDEHKFNELAGFTKE
                     DYRMPKAFTNREIDSTNQVFDIDKDELDELRLWHDKNKIIYR"
     assembly_gap    2968..3067
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3068..3345)
                     /locus_tag="PLB33_08180"
     CDS             complement(<3068..3345)
                     /locus_tag="PLB33_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOG63541.1"
                     /translation="MKKILRINLRTKQITYQDASREYLETGGRGLIAKIMLNEVNPLC
                     DPLGRENKLIFANGLLTGTNVSSASRIAIGAKSPLTGGIKESNGGGITA"
     gene            complement(3501..5588)
                     /locus_tag="PLB33_08185"
     CDS             complement(3501..5588)
                     /locus_tag="PLB33_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG63542.1"
                     /translation="MGYGSMFEKELNKLIESEDNIECIKDIISNDISNTEQNKEYIER
                     LLEFSTENKLYRSEAWGYYFKGWHYIDNSEYEKAVENFMISYELFDKLNNKYEIAYAC
                     NGLTNVYCLMGQYKLSNEWGLKGISFCEETKNSKAMIILLINTCINYIQMEYYDKALE
                     IIESIEEIDYDLTKSQEISHMLAKAEIEINIGNPDKALKNIEQAMKIESVNNIKSTMG
                     EMLKLKGMAYLKTGKYDLAEDQFKKSYNFCVKYDLIYEKCSTMLQWSKLCILTDNYEK
                     AIDLLNQIIIVGSSNKFNTIMREAFHSLYSIYKKLEDTEKALNYLEKYIKINNEMYDY
                     EQSQLMAEINLNNTKRMAEQYKQLYKRTELLSTIGQKIISNLDISSIIRIINEEIKKL
                     IDLDYFGIAVYDSENNRSEYYFMRDYKIVCSTVNYDEENTLGGYCIKNKEDIIIGNEK
                     LEFKKYLYSEPNYIRGFEEEGVKISSIIYTPMIINDKVVGLMTAQSEKENLYGKDELN
                     ILKILANYTAIAVENAKVYKEIENIATYDSLTNFLTKNEILKIGDSIYEKAKCNNQKF
                     TVIMMDLDDFKIVNDTYGHVYGDKAMSLIAETMSKCIRNTDYIGRFGGDEFLLICTGT
                     GLEEALYVAERIRKTIQSSIFVLSDEIKVRVTTSLGVHEYDENDNSFTDVVKRADQCL
                     YLAKETRNTVMSK"
BASE COUNT         1634 a         1091 c          817 g         2151 t
ORIGIN      
        1 aaatttattc aacatgccgg aatatataat attatctatt attcctttgt ccctgactaa
       61 gggtaatttt tcgatttcat cgcttaaaaa tgacaaatta agcattaaaa gattattagc
      121 gtattcttcc ttgttttcaa tatttaattt attaaatgga ttatatgatt tattttctaa
      181 atccttcttc atatcttcat aagcatctat gaagtaaata tattttccaa gatagaaacc
      241 tatttttctt aagctttgct cccagtggtc ttttttatat aaaaatattt cttccatcaa
      301 atggccgaat tcgttggata cggcatcgat atcttccgtg tcttcttttt caagctttga
      361 aatgttatta agcctcagtt taattgtttt tgtaatatca ttaagattag atacggattt
      421 ttgtaattga ctctgcaata ttttagccgc tgccaatgac ttgtaatccc tgtcatcaac
      481 ccagttatca agcatattgt aatatgaaag caagacattt aaagaagcgg catattctgt
      541 tatttcattt tgtataataa gctgcttatt tttgggatga actatgcacc tttcattata
      601 aattttattt tcgggttcat agagggagct taacaaaagt attaagaaag tcatatcata
      661 attcaatgtc agccttgatt tattcccgta cattgttttt aaacatttgc acagaccgca
      721 gtaataaccc ttgtagctgt aatattcctt gaattttaat tccattttat ttatcgttac
      781 gtaaccgaac atttactcac tcatttctat gttgttatta aattgtatca attcctcctt
      841 gctttttcaa tacaaatttt aacctcagag agctaagaat tatcacgtaa ttacaacacc
      901 acataataaa tacgccttgt caccctgagc gtaagcgaaa gatttgttaa ttataaaaaa
      961 tgttaataaa ttatacaatt gacattcatg tatttggata atattatata taaatacaaa
     1021 taatgaagca tagaattagc ttttgcattt atataatcat tgtgtcttgt agagcaagca
     1081 ataacagctt gcacttaggc acttatgata gaatgagtat ccggggaggt agaaatgaat
     1141 acggataaaa aagagataac cagtgcttta actcaccttg gaggagcaat tttcgggata
     1201 attgccttaa ttttgttgtt gagtgctgct ataaaggaag acagtttaat cgccttagca
     1261 tcatttttaa tttttggttt aagcatgata tttttatatt gcacctcttg tatttaccat
     1321 tttattgaca gctcaaagaa aaaggcaaag gtaataatga ggaaacttga ccacatcatg
     1381 attttcgtat tgatatcagg tacatataca cctgtatgcc ttttaatatt aacaaaaaat
     1441 ataggttaca aactacttgc cctagtttgg tcgattacaa taataggagc attaattaaa
     1501 atattttgga tagatgcccc gcgatgggta agtgcaggat tatatttagg catgggatgg
     1561 atgtcagtac ttgtttttat gcccttggta agaagcatgg aaacaggcgg aatcatctgg
     1621 ctttgtctcg ggggactatt atacacagcc ggcggaataa tttacggttt aaaaaaaccc
     1681 gccataaata aacaatggtt cgggtttcac gaattgtttc atatatttgt attagcaggt
     1741 tccttctgtc actttatgat gatgtatttt tatgtagctt aatttattaa ggtcatccat
     1801 agcgtaagca aatgacacag gcttctagcc gccgctcagt atgggaagga agaatacttc
     1861 tgaattatct tcgactttat ctgagaattt ctttggaata ccgttgacaa gcacagcacc
     1921 tatgtgctgc ttgtcaattt ttaattccgt aattatttca tcaatcgtaa tatctttata
     1981 gtctaaatca atattctcca catcgctgtc aacggtagat aattttattt ttatcatgcc
     2041 acaacctcaa ctcatccagt tcatccttat caatatcaaa aacttgattc gtactatcaa
     2101 tttccctgtt tgtaaatgct tttggcatac gatagtcttc tttggtaaaa cctgcaagtt
     2161 cattaaattt atgctcatct cttatagctt cgagagctat atcttcaagc caatccatat
     2221 ctaaatccca gccgtgcact gcatttacca agtccacgaa taggtccata tgttttgcaa
     2281 cagctcctct taaaaacata catactccca aacagtctat ttttgccatc ataatttgtg
     2341 ctgcttttga tgcttcgaca gcatcctccg cctttaccac gggaccgtaa gtatggtccg
     2401 ctcccattgg tgatgttgca aatgtaactc ccattgcctt tatggctctc gggtcataag
     2461 caggcattgc ttgaccgttt ataacaggga tattttttac tcccagtgcc tttcccgcaa
     2521 taagtgttcc ttccccgagc agtttcccga gctctgtacc ggtttttatt tcatcaagca
     2581 aatttaaagc tgcttcatta tcgccaaatt ccacaatacc tgcttccatt gccattccca
     2641 tgacagctcc tgtctcgatt gtgtcaactc ctaaatcatt gcagactgca tttagttttg
     2701 caattgtgtc taaatctcct attccgaggt tggagcccat caatccgatt gtttcatatt
     2761 ccaatggaga tactattgtt tttccttctt cgtcgggaac aacattagag cattgaatta
     2821 tgcagccagg catacatgca tgagcagtct ttccttctcc gcccctttct tttataagct
     2881 catataagct ctgccctgaa atttcttctg catgctcaaa ttttgcacct ttgaagttcg
     2941 ttaccggaag tcctgtcaaa tcatttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnngct gtaattcctc ctccgttgct ttccttgatt ccgcctgtta agggactttt
     3121 agctccgata gcaattctgc ttgcactgga cacatttgta ccggtcaata gtccgttggc
     3181 aaaaataagt ttattctctc tgcctaaagg gtcgcataga ggatttactt catttagcat
     3241 tatttttgct attagccccc tgcctcctgt ttctaaatat tcccgagaag catcttggta
     3301 agtaatttgt tttgttctta aatttattct cagaattttt ttcatattgc tctcctattt
     3361 aaaatgtcaa aattagtgta cccctcagca ataaatttaa tcataataat tttgacactg
     3421 ttatgtctaa tttggaccca aaggggacgg ttgtccactg agaaccgtcc cctccgggtt
     3481 ttcccctccg ggtttgttat ttatttactc ataacggtat ttcttgtttc ttttgcaaga
     3541 tagaggcatt ggtctgctct ttttaccaca tcggtaaatg agttgtcatt ctcgtcatat
     3601 tcatgtaccc ctaaactcgt tgttaccctt acttttattt cgtcacttaa tacaaatata
     3661 ctgctttgta ttgtttttct tattctttcg gcaacgtata aggcttcttc taatcctgtt
     3721 ccggtacata tgagcaaaaa ctcatctcct ccgaatcttc ctatatagtc agtattccta
     3781 atgcattttg acatggtttc ggcaataaga ctcattgctt tatctccgta aacatgaccg
     3841 tatgtatcat ttactatttt aaagtcatcc aaatccatca taattacggt aaatttttga
     3901 ttgttgcact tggccttttc gtaaatacta tctccaattt tcagtatttc atttttagtc
     3961 agaaaatttg tcagactgtc ataagttgca atattctcga tttctttgta taccttggca
     4021 ttttcaacag caattgcagt ataatttgct agaattttca agatattcaa ttcatccttg
     4081 ccgtaaaggt tttctttttc actttgagca gtcatcaatc cgacaacttt gtcatttata
     4141 atcatgggag tataaataat ggaagaaatt tttacgcctt cctcttcaaa tcctctgata
     4201 tagttgggtt cagaatataa gtatttctta aattccagct tttcatttcc gataataata
     4261 tcttccttat ttttaataca atatcctccc agagtatttt cctcatcata atttacagtg
     4321 ctgcaaacta ttttatagtc tctcataaaa taatattccg accggttatt ttcactgtca
     4381 tacacagcaa ttccaaaata atcgagatca atcagctttt ttatttcttc gtttatgatg
     4441 cggataattg aactaatatc taaatttgat attattttct gccctatggt tgaaagaagt
     4501 tcagtccttt tataaagctg tttatattgt tctgccatac gttttgtatt gttcaaattt
     4561 atttcagcca taagctggct ttgttcataa tcatacattt cattatttat ctttatatac
     4621 ttttcaaggt aattcaatgc cttttcagta tcttccagtt ttttataaat agaatataaa
     4681 gaatgaaatg cttctctcat tattgtgtta aacttatttg atgaaccgac aataataatt
     4741 tgatttaata agtcaattgc cttttcataa ttatcagtca aaatacataa tttagaccac
     4801 tgcagcatgg tggagcactt ttcatatatc aaatcatatt taacacaaaa attataagac
     4861 tttttaaact ggtcttcagc taaatcatat ttacctgttt ttaaataagc cataccctta
     4921 agttttaaca tttcacccat ggtagattta atattattta ctgattctat tttcatcgct
     4981 tgctcaatat ttttaagggc tttgtcgggg ttgccgatat ttatttcaat ttcggctttt
     5041 gccagcatgt gcgaaatttc ttgacttttt gttaaatcat aatctatttc ttcaatactt
     5101 tctataattt ctaacgcttt gtcataatac tccatttgaa tatagttaat acatgtattg
     5161 ataagaagga taatcattgc cttagagttt ttcgtttctt cacaaaacga aatgccttta
     5221 agaccccatt catttgataa tttatactgt cccatcaaac aataaacatt tgtcaatcca
     5281 ttgcaggcat acgctatttc atatttattg tttagcttat cgaacagctc ataagaaatc
     5341 ataaaatttt ctactgcttt ttcatattca gaattatcga tataatgcca gcctttaaaa
     5401 taataacccc acgcttcgga tctgtatagc ttattctctg tggaaaattc caacaatctt
     5461 tcaatatatt ctttattctg ctctgtattg cttatgtcat tcgaaattat atcttttatg
     5521 cattcaatat tatcttcgct ttcaatcagt ttatttaatt ctttttcaaa catactcccg
     5581 taacccaatt ttatcctcct tgagaatcga ttagaaatat aatatattga acgctaaaaa
     5641 ttgtcaaata cttattacaa aaaaaccttg cggactcccg caaggttttt gattttcttt
     5701 attattcagc taatcttgct ttagcttgat aatttgctat atactcttcc aattctttcg
     5761 gtgatttctt aaccttcatt ttttgcttat att
//
LOCUS       DAOSHP010000098         5762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12303_length_5762_cov_8.953391, whole genome shotgun sequence.
ACCESSION   DAOSHP010000098 DAOSHP010000000
VERSION     DAOSHP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12303_length_5762_cov_8.953391"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..664)
                     /locus_tag="PLB33_08190"
     CDS             complement(164..664)
                     /locus_tag="PLB33_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOG63543.1"
                     /translation="MKRNNRFITRTALIAAVYAALTYAFAWMSYEQIQFRISEVLVLF
                     AFIEPRYGLGLVLGCILANLASPFGVIDIAVGSFATFLAIVFIVKIRKLFGLNKKALI
                     IASLGPVITNALLVGAELTYLFNTPFLLNALYVGAGEFAVVVFIGTVVVNLIMKNNVL
                     VEKLSF"
     gene            complement(807..1196)
                     /gene="rpsI"
                     /locus_tag="PLB33_08195"
     CDS             complement(807..1196)
                     /gene="rpsI"
                     /locus_tag="PLB33_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOG63544.1"
                     /translation="MEAQYRGTGRRKKAIARVRLVPGTGKININERDLDDYIKYETLR
                     NLVREPLMITDTFGQYDVLVNVKGGGYTGQAGAIRHGISRALLQVDKELRPLLKKAGF
                     LTRDSRMKERKKYGLKKARRASQFSKR"
     gene            complement(1215..1643)
                     /gene="rplM"
                     /locus_tag="PLB33_08200"
     CDS             complement(1215..1643)
                     /gene="rplM"
                     /locus_tag="PLB33_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOG63545.1"
                     /translation="MKSFLAKPNEVERAWYVIDAEGKTLGRLATEVAKLLRGKHKAIY
                     TPHVDTGDFVIVVNAEKVVLTGKKLDQKLYRHHSLYPGGLKEVPYRNLFRSKPTQPVY
                     LAVKGMLPKNSLGRKMLKKLKVYAGPEHNNQAQKPQVYEF"
     gene            complement(1815..2555)
                     /gene="truA"
                     /locus_tag="PLB33_08205"
     CDS             complement(1815..2555)
                     /gene="truA"
                     /locus_tag="PLB33_08205"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOG63546.1"
                     /translation="MVRNIKLLIAYDGTAYHGWQTQLNKVTIQETIEKALSIVMKQKI
                     DLTGSGRTDSGVHALGQVANFKADTKIPEDKIKIALNANLPPDIRILESVDVDMSFNA
                     RFDALDKTYMYQIYNDRVGNPFYSRYSCFIPQSLDIDKMKEALKLIKGTHDFKGFMAS
                     GSYVKTTVRTIYDTELIKEENLLKLYIKGDGFLYNMVRIIAGTLIDIGKGIKSIDCIE
                     DALINKNRKVLGQTAPPQGLFLMKVNYK"
     gene            complement(2549..3346)
                     /locus_tag="PLB33_08210"
     CDS             complement(2549..3346)
                     /locus_tag="PLB33_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOG63547.1"
                     /translation="MFKNLTIGQHYPVDSPIHNLDPRLKIIMTFIFIISLFLIESFTG
                     FIFVIIFLTVIITISKVPVKFVIKGLRPILFIILITFIINLLMTPGRVIYTIGVIKIT
                     EEGLRQAGFMAVRLTLLIMGTSMLTLTTSPIILTDGIESLLKPFKRFGLPAHELAMMM
                     TIALRFIPTLMEETEKIMKAQKSRGADFESGNIMSRAKNLVPLLVPLFISAFRRADEL
                     AMAMEARCYRGGENRTRMRQMKISKGDYAAAFVFTVYFAAVLIIKIW"
     gene            complement(3339..4199)
                     /locus_tag="PLB33_08215"
     CDS             complement(3339..4199)
                     /locus_tag="PLB33_08215"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOG63548.1"
                     /translation="MSIRTENIKYIYSEGTPFRTLALDNVSVTINQGEFVGIIGHTGS
                     GKSTLIQLFNGLELPTEGKVEVDGTIVGKDKKELKLIRQTVGLVFQYPEYQLFEETVA
                     KDVAFGPMNLNISQQEIDFRVKEALELVGFNYNKIKDVSPFELSGGQKRRVAIAGVLA
                     MKPQYLILDEPTAGLDPAGRNEIFNQIKKLHEESKFTVVLVSHSMEDIAKLVDRVIVL
                     SKGKIHMEGSPKEIYSQAEELEKIGLGIPQTASIVRELKNRGFNIKQDILTIEEAKEE
                     ILKEVRRRNV"
     assembly_gap    4222..4321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4322..4845)
                     /locus_tag="PLB33_08220"
     CDS             complement(<4322..4845)
                     /locus_tag="PLB33_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG63549.1"
                     /translation="MIEYIIKIDNVKFKYDKNSSYAVNGVSLNIKSGEFTAIIGRNGS
                     GKSTLAKLINSILLPTEGKIFVKGMDTSDDSKLWDIRQTAGMVFQNPDNQLVATIIEE
                     DIAFGPENLGVQPEEIRRRVDNALNTVGMYEYRKRPPHLLSGGQKQRIAIAGILALNS
                     ECIILDEPTAMLDPS"
     gene            5201..5668
                     /locus_tag="PLB33_08225"
     CDS             5201..5668
                     /locus_tag="PLB33_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63550.1"
                     /translation="MFCLRNKINICDFKPDIAQNLGNLSLTVRNRSSNEPVAFAEVSV
                     YYLTIYGVYGEGGEASMMVRHITDENGRIPVIELPVIDRINQPNNSYFVTVRHFRYYP
                     VNIMNIPIYPDITTEYNVMITPLTEQHPEYEFHISPELIRQRENLQNNYKTLF"
BASE COUNT         1641 a         1152 c          869 g         2000 t
ORIGIN      
        1 aaccgaaaat cattcatttg tgtaaatttg gggatagttc aattttttct catccggtgc
       61 aagtattcgg tgcctgaagt attcggtgcc agggaaacct ccatattttc ggaggtttcc
      121 ctggcaccgg ataaatggca ccggataaag aaactgtcct cgtttagaat gaaagttttt
      181 caaccaggac attgtttttc attattaaat ttacaacgac agtgcctatg aaaacaacta
      241 ccgcaaactc gcctgctcca acatataatg cattcaataa aaagggagtg ttaaacaaat
      301 aagttaattc tgctcctacc agtaaagcgt ttgtaattac aggtcccaaa cttgctataa
      361 tcagcgcttt tttattcaat ccgaataatt ttcttatctt tacaatgaat actattgcca
      421 aaaatgttgc aaatgagcct accgcaatgt cgattacccc aaaggggctc gctaagttag
      481 ccaatatgca tcccaatact aagccgagac catatcttgg ctctataaat gcaaacaaga
      541 ccaatacttc tgatattcta aactgtatct gctcgtagct catccatgca aaggcatagg
      601 tcaaagcagc ataaaccgcc gctataagag cagtgcgtgt tataaaccga ttgtttcttt
      661 tcattaaaaa aactcctttc gaatatattc tcggagtcac taaaaaacct tgatttttta
      721 agtgggtgcc aagaaaacac tatagattaa gtttttaagg gacggtcctt tttgttttct
      781 taaaaaagaa tcgtccccaa atatttttat ctcttgctga actgtgaagc tcttcttgct
      841 tttttcaaac cgtatttctt tctttctttc attctggaat ctcttgttaa gaatccggct
      901 ttttttagca agggtcttaa ttccttgtct acctgtaaaa gcgctcttga tataccatgt
      961 ctgattgctc cggcctgacc tgtatatcct ccgcccttta catttaccag tacatcatat
     1021 tgaccgaatg tatcagtaat cattaaaggc tctcttacaa ggtttcttaa agtttcatat
     1081 ttgatataat catctaaatc tctttcattt atgtttattt tccctgtccc gggaactaat
     1141 cttactcttg ctatcgcttt ttttcttctg cctgttcctc tgtactgtgc ttccatcgta
     1201 ttcctcctct cccattaaaa ctcgtatact tgcggttttt gagcctgatt attgtgctcg
     1261 ggacctgcgt atacttttaa tttctttaac atttttcttc caaggctgtt ctttggaagc
     1321 attcccttta ctgccagata aacaggctgt gttggtttac ttcggaataa attcctgtaa
     1381 ggtacttcct tcaaaccacc cggatataga gagtggtgtc tgtataattt ttgatctaat
     1441 tttttacctg tcaatacaac cttttcagca tttactacta ttacaaagtc tcctgtatca
     1501 acatgcggtg tatatatagc tttatgttta cctctaagaa gtttggcaac ttctgttgca
     1561 agtctaccta atgtcttacc ttcggcatct atgacatacc atgccctttc aacttcatta
     1621 ggtttagcta agaagctttt cattttcttt cctcctgtaa ttctactgta ttaaattatc
     1681 tgtttatcta ttcaaaaaac ctaactccgg ggcattggaa cggttttttt ctcttttcat
     1741 acctaaagta ttttatatta aataaattat aatgtcaagc acttttcagg cactttttca
     1801 ttaatatccc ggctttattt ataatttacc ttcattaaaa acaacccttg gggaggagcc
     1861 gtctgcccta atactttcct gtttttatta attaaagcat cttctatgca atcaatgctt
     1921 tttattcctt tcccaatatc aattaaggtt cctgcaataa ttcttaccat attgtacaaa
     1981 aatccatcgc ccttaatata gagttttaac aaattttcct cttttataag ctcggtatcg
     2041 taaatagttc tcaccgttgt ttttacatag gagcctgaag ccataaatcc tttgaaatca
     2101 tgggttccct taatcaattt aagggcttct ttcattttat caatatccaa agactgcggg
     2161 ataaaacatg aataccttga ataaaacgga ttcccgaccc tatcattgta aatttgatac
     2221 atatatgttt tatcaagtgc atcaaatctt gcattaaaac tcatatcaac atcaacagat
     2281 tccaatatcc ttatatccgg gggcaaattt gcattaagcg caattttaat cttgtcttcg
     2341 ggtattttgg tgtctgcttt gaaatttgca acctgcccca aggcgtgaac gccgctgtca
     2401 gtccttcctg agcccgttaa atcaattttt tgcttcatta ctatacttaa tgccttttca
     2461 atcgtttctt gaattgtaac cttatttaat tgggtttgcc acccgtgata agcagtaccg
     2521 tcataggcaa ttaaaagttt aatatttctt accatatctt aattattaat actgctgcaa
     2581 aataaaccgt aaaaacaaat gctgcagcat aatcaccctt tgatattttc atttgcctca
     2641 ttcttgttct gttctctcct ccacgatagc atcttgcttc catcgccatt gccaattcat
     2701 cggcacgtct gaatgcactt atgaaaagcg gaaccaagag aggaactaaa ttttttgccc
     2761 tgctcataat attgccgctt tcaaaatcag cacctcttga tttctgtgcc ttcattattt
     2821 tttctgtttc ttccataagg gtaggaataa agcgaagagc aattgtcatc atcattgcca
     2881 gttcatgtgc aggaaggccg aatcttttaa acggctttaa cagactttcg ataccgtcag
     2941 tcaatataat gggcgacgtt gtcagtgtca gcatagatgt tcccattatt agaagagtaa
     3001 gtcttacagc cataaatcct gcctgcctca aaccttcttc agtaatcttt attactccga
     3061 tggtatatat aactcttccc ggcgtcatca aaagattgat aataaatgta attaatatga
     3121 tgaataatat gggtcttaat cctttgatta caaatttaac gggtactttt gaaatagtaa
     3181 taataacggt caaaaatata ataacaaata tgaaccctgt aaagctttca atcaaaaata
     3241 atgaaattat aaaaataaat gtcatgatta ttttaagcct ggggtcaagg ttgtgtatgg
     3301 gagaatcaac aggataatgc tgtccgatag ttaaattctt aaacatttct tctcctcact
     3361 tcctttaata tttcttcttt tgcttcttca attgtgagta tatcctgttt aatattaaat
     3421 cctctattct taagctccct tacgatagaa gctgtctgag gaattccaag gcctattttt
     3481 tccaattctt cagcttgtga ataaatttct tttggactgc cctccatatg tattttcccc
     3541 ttggataaaa ctataactct gtccaccagt tttgctatat cctccatact gtgtgatacc
     3601 aacacaacgg taaatttaga ctcctcatgc aattttttta tttgattaaa tatttcattc
     3661 ctgcctgcag ggtcgagacc ggcagtgggc tcatctaata taaggtactg cggtttcatt
     3721 gcaagtacgc ctgcaattgc aactcttctt ttttgacctc ccgataactc gaagggagat
     3781 acatctttta ttttattata attaaaaccg actaattcca atgcctcttt tacccgaaaa
     3841 tcaatttctt gctgggagat atttaaattc attggtccaa aggctacatc ctttgcaact
     3901 gtttcttcaa aaagctggta ttcggggtat tgaaatacta atcctactgt ttgacgaatc
     3961 aactttaatt ctttcttatc tttgccgact attgtgccgt caacttcaac tttaccttca
     4021 gtgggaagct cgaggccgtt aaaaagctgt atcaaagttg atttgccgga gccggtatgt
     4081 ccgattattc ccacaaattc gccttggttt atcgtaacgc tcacattatc caatgccaag
     4141 gttctaaacg gagtgccttc actgtatata tattttatgt tttctgttct tattgacata
     4201 atatgtccac caattccttt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 ngaagggtct aacattgcag taggctcatc caatattatg cactccgaat ttaaagctaa
     4381 tatacctgct atggcaattc tttgtttttg tcctccagat aagaggtgag ggggtctttt
     4441 tctgtattca tacatgccta ctgtatttaa ggcattatca acccttcttc taatttcttc
     4501 aggttgcacg cctaaatttt cgggaccgaa tgcaatgtct tcctcaatga tggttgccac
     4561 taactgatta tcaggatttt ggaacaccat accggctgtc tgtcttatat cccaaagttt
     4621 agaatcatct gaggtatcca ttcctttaac aaaaattttc ccttcggtag gaagtaaaat
     4681 tgaatttatt aatttagcca gcgttgattt cccagagcca ttgcggccta taattgcagt
     4741 aaactctccg ctttttatat tcagggaaac tccgtttact gcataagagg aatttttatc
     4801 atatttaaat tttacattgt ctattttgat tatgtattct atcattttac acccttattt
     4861 gaatttttgc gttaaaagct gcataagaat aatatcatca ttcgcattat tttacaacag
     4921 taatttacac taatgttacg gttatcggtt tctttggaat tacaaaaaaa taaaaggctg
     4981 agcaaagtat atcactctgc gcacctttta ttttattcgt ttaataataa ttcatcatcc
     5041 tgttttcaca caatatttat gataacaaaa gagcgccttt ggaattttct aaaattattg
     5101 tataattatg cgcccactcc aaaagcactg aacttgttaa tataatcaat aattcataat
     5161 tttcatataa taggttataa ccatatggga ggaaatataa atgttttgtt taagaaataa
     5221 aataaatata tgtgacttta aacctgacat tgcccaaaac ttaggtaatt tatccttaac
     5281 tgtgcgtaac agatcaagca atgagcctgt tgcttttgct gaagtaagtg tttattattt
     5341 gactatatat ggagtttatg gcgagggagg agaagcaagt atgatggtaa ggcacataac
     5401 ggatgaaaac ggcagaatcc ctgttattga actgcctgtc attgacagaa tcaatcagcc
     5461 taataactcg tactttgtta ccgtaaggca tttcagatat taccctgtta atataatgaa
     5521 tataccgata taccctgata ttaccaccga atacaatgtg atgataacac cgctgaccga
     5581 acaacatcct gaatatgaat ttcatataag tccggaattg atacgacaac gggagaattt
     5641 gcaaaacaat tacaaaacac tgttttaacg aggcgtcttt acccgcctcg ttaaagtata
     5701 tatacagctc tttcatttca tttatatgtc atgctaatct cttactatta taaaattatc
     5761 tt
//
LOCUS       DAOSHP010000099         5749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12340_length_5749_cov_8.183000, whole genome shotgun sequence.
ACCESSION   DAOSHP010000099 DAOSHP010000000
VERSION     DAOSHP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12340_length_5749_cov_8.183000"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..674
                     /locus_tag="PLB33_08230"
     CDS             72..674
                     /locus_tag="PLB33_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOG63551.1"
                     /translation="MKYEKIIKALIHNGYKVSYFENSNEAAKYIVDNVKNTTVGFGDS
                     ATLESINLAELLSKNNTVIDPAPYSGDEFNEVAKKALLTDVFFTSVNGVAETGELVNI
                     DGTGNRVAGSLFGHKKIYFVFGTNKIEPNLEKAIWRARNIAAPQNSKRLGYNTPCAVK
                     ADRCYDCSSSERICNTLNIHLKKMKYVEAECIIINENLGM"
     regulatory      760..934
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1066..2178
                     /gene="cobT"
                     /locus_tag="PLB33_08235"
     CDS             1066..2178
                     /gene="cobT"
                     /locus_tag="PLB33_08235"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOG63552.1"
                     /translation="MHVEFYKNFNIKEASEKELESVLNEIIKGIKKPDSISAEGMQKR
                     LDAKIKPLRSLGVLEDIAVQLAGIQGTEFPEVKGKAVLLMAGDHGVVKEGVSAAPQEV
                     TAQLYYSYLNGGGGINVLTGHAGAKLICNDIGICGRLEPPDLMANRIKNGTDNIAEGP
                     AMTREEALRSLLTGAKIASEAIESGINILATGEVGIGNTTPSAALITVLTGCSVEEAT
                     GSGTGLKGDALKHKCNIIKKAIEINKPNLNDPIDILSKIGGLEIAAMVGAILEAAYQN
                     VPTILDGIISSAAALIASKLNPIVVEYMIPSHSSEEKGELVALKHMGLEPRLFLNMRL
                     GEGTGAALMFHIVEAAVKIADEMATFETSGVTTGDF"
     gene            2302..2889
                     /locus_tag="PLB33_08240"
     CDS             2302..2889
                     /locus_tag="PLB33_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63553.1"
                     /translation="MKNRHIYIILTRTNTVLSNLISIIKNDEYTHASISLNKNLNTMY
                     SFGRKYTYNPFIGCFVKEDLYEGVFGFHNKLKGQVLELNATEEQYDKVVKLLDEFILN
                     SKYLKYNYLGLINSLFNRESICEDRFLCSEFVYYILNKAGIAELNKPRNLVRPQDLLN
                     LKSRIIFKGNLKSKKFIKKYENSFIEKYSAVGTLS"
     gene            3018..3476
                     /locus_tag="PLB33_08245"
     CDS             3018..3476
                     /locus_tag="PLB33_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOG63554.1"
                     /translation="MKTEHKEITGKGHNFEYIVSACLCGEKTRYDGKVFVSDKIKRLV
                     DEGKAITVCPEVLGGLKVPRLPCEIKNSRVINIASDDKTEHFVDGAFKTLEIAKKYGI
                     KKAILKEYSPSCGSKYIYDGTFTKTLIPGQGITTRLLQLNDIEVISDQEF"
     gene            3582..5228
                     /locus_tag="PLB33_08250"
     CDS             3582..5228
                     /locus_tag="PLB33_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOG63555.1"
                     /translation="MQTKYLKRIIAFVIVLSMVFTTVAMAMPYGQAKKLIKNKYSWAQ
                     VQKYVKGRGIMKGYPDGMFRENDKVKRADAIIMIDRAFKLSALIDIINRDYKDVFNDV
                     DKNEYYFDAVHIARLLGITKGKGNNNFHPKQAITIREAILLIERAMDRNKYFDFDDDI
                     DLWKEYEEKELDKSATRGDIAAMLYYVLTGSKYKGVPQEYDLEEISLTIEKDEILRFD
                     DEYGKKDSIIESIEDMVDDLEYVQFTKPLNKDNTFLYYDYNSRSRSNSFVTNRNKYYI
                     DPDDEQPDLSKVSVVPKTSGVLEIEYIAYDEDGESYKGLIKITVEGKYDDLDDITFTG
                     YENTPIKFMISEFKKVFNKEFEYTPDDEISFELPDEKFGTLYFDENKDGELKDAEEIE
                     AKNSFKLKQLNFIYFNPAEDYVDEDEPVEIKYTVEDQEGNLYEGKINITVKSLLGELT
                     IDEDDDFGIEVKDYLDEFDDYDFQKIKFEKADKDDKSTLTIETNTGTRSVIGMELNIR
                     RLQDIEFEPEKDFDGTTFKYTVTTDDGEQFIGLVTVEMDD"
     gene            5370..5663
                     /locus_tag="PLB33_08255"
     CDS             5370..5663
                     /locus_tag="PLB33_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HOG63556.1"
                     /translation="MAKFNETVEKLIPTLDKFVPIVARVHGGHHPEFHDVRKIFDVIK
                     EKVKEAGAEKPNLSEEFAELREITDNYTVPGDVCESYEAVYKMLAELDEAYQA"
BASE COUNT         2166 a          763 c         1172 g         1648 t
ORIGIN      
        1 ttgtccttta tattctgcca tggtataatt taacaggaac atatttgatt gtttgggagg
       61 aggaaggttt gatgaaatat gaaaagataa ttaaagcatt aatccataac ggctataagg
      121 tttcgtactt tgagaattca aatgaagcgg caaaatatat tgtcgataat gtaaaaaata
      181 ctacagtcgg ttttggtgat tccgctacac ttgaatctat aaatttagct gagcttttat
      241 caaaaaataa tacagtaata gacccggcac catattccgg agatgaattt aacgaagttg
      301 ctaaaaaagc gcttctgaca gatgtatttt ttacgtcggt caacggtgta gctgaaaccg
      361 gagaactggt aaatatagat ggaacaggaa atcgtgtagc aggttcgctg tttggacata
      421 agaaaattta ctttgttttc ggaacaaata aaatcgagcc taatttagaa aaggctatct
      481 ggcgtgcaag aaacattgct gctcctcaaa attcaaaacg tctgggttat aatactccgt
      541 gtgcggtaaa agctgaccgc tgctatgatt gttcaagttc ggaaagaata tgcaacacgc
      601 tgaatattca tcttaaaaag atgaaatatg ttgaagcaga gtgtataata ataaatgaaa
      661 atttaggaat gtaaaaaagc gttactgata acatccaact gttattagcc tgagtgttaa
      721 ttaaaataat tgacatggtt taatctttgt attagaatag cattaataaa attcataggt
      781 gcctaagaag gagaataggg aaaccggtga aaatccggta cgtacccgac actgtgaaga
      841 ttagtccttt actataccac tggtatatgc cgggaaggag taaaaggacg tttgagtctg
      901 agtcaggaga cctgcctatg aatagaagtc tttcgattga aagcaaacgg cgatgaaggt
      961 aaagtcccga acataataat tttattatgt cgggattttt ttgttggttg ccgggaaact
     1021 attattgttt gatgagagtt acatatatga aattggaggt aaagaatgca cgtagaattt
     1081 tacaagaatt ttaacattaa agaagcaagt gaaaaagaat tagagtcggt attaaatgaa
     1141 attataaaag gaattaaaaa gcctgattca atttcagccg aaggaatgca gaagagactt
     1201 gatgccaaga taaaaccgct tagaagtctg ggtgttttag aagacattgc cgttcagctg
     1261 gcaggcatac aaggcaccga attccctgaa gttaagggaa aagcagtact tttaatggca
     1321 ggggaccatg gtgttgtaaa agaaggagtg agtgcagcgc cacaagaggt aactgctcaa
     1381 ttgtattata gttaccttaa cggaggaggc ggtattaacg tattaaccgg acatgcaggc
     1441 gcaaagctta tatgcaatga tataggtata tgtggtcgtc ttgagcctcc ggatctaatg
     1501 gcaaatcgta taaaaaacgg cacggataat attgccgaag gtcctgccat gacaagggaa
     1561 gaagctttaa gaagcctgtt aaccggtgca aaaattgcat cagaagcaat cgaatccgga
     1621 ataaatattt tggcaaccgg ggaggttgga atcggaaata ctactccaag tgcagcttta
     1681 atcacggtat taacaggatg cagcgttgaa gaagctacag gcagcggtac cggattaaag
     1741 ggtgatgcat taaagcataa atgtaatata ataaaaaagg caattgaaat aaacaaacct
     1801 aatcttaatg acccgataga tatattatca aaaataggcg ggttagaaat tgctgcaatg
     1861 gttggtgcaa ttttagaagc tgcttatcaa aatgttccaa cgatattgga tggaataata
     1921 tcatccgctg cagcacttat agcatcaaaa ctcaatccga ttgttgttga atatatgata
     1981 ccatctcatt catcggagga aaaaggtgaa ctggtagctt tgaaacacat gggattagag
     2041 ccaagattat ttttaaatat gagattaggg gaaggtacag gtgctgcgct tatgtttcac
     2101 atagttgaag cagccgtaaa aatcgctgat gaaatggcta cctttgaaac ttcaggagta
     2161 acgaccggag acttttaatc ttgtggaagt tagcgataat ttcattaagt aaattaaata
     2221 tgattttaat gaatcattaa tatatttttt atatatattt aagtctgttt tgccataatc
     2281 aatccgtacg taggagggat tatgaaaaac agacatattt atattattct aacaagaacc
     2341 aatacggtat tatcaaatct tatcagcata attaaaaatg atgagtacac acatgcttct
     2401 atatccttaa ataagaattt aaacaccatg tatagttttg ggagaaaata tacctataat
     2461 ccatttatcg ggtgtttcgt gaaagaagat ttatatgaag gagttttcgg atttcacaat
     2521 aaacttaagg gacaagtatt agaacttaat gccaccgaag agcaatatga taaggttgta
     2581 aaattattgg atgagtttat tttaaacagc aaatatctaa aatacaatta tttaggactg
     2641 ataaacagtt tgtttaatag agaatccatt tgcgaagaca gatttttatg ctctgaattt
     2701 gtatattata ttttaaacaa agccggtatt gcagaactta acaaacccag aaatttagta
     2761 aggccacagg atttattaaa tctgaagagc aggattatct ttaaaggaaa tttaaaaagt
     2821 aaaaaattca taaagaaata tgagaatagt tttatagaaa aatattcggc ggtgggtact
     2881 ttatcataac taaaaaatta tttggataag tccgataaaa tggtaaaata ttattatatg
     2941 tggtaaaata tattaaattt tatatggaga tataattgat gaatacggat gcaggggaag
     3001 aaggtgattt gttgaatatg aaaaccgagc ataaagaaat aacagggaag ggacataatt
     3061 tcgaatatat tgtaagcgca tgcctttgcg gagaaaaaac aagatatgat ggaaaggtgt
     3121 ttgtttccga taaaatcaaa agattagttg atgaaggaaa agcaattacg gtatgcccgg
     3181 aagtattggg cggcttaaag gtgccgaggc ttccttgtga aataaaaaat agcagagtaa
     3241 ttaatattgc ttctgacgat aagacggagc attttgtgga tggtgctttc aaaacgttgg
     3301 aaattgctaa aaaatatggc ataaaaaaag caattttgaa ggaatatagc ccatcctgcg
     3361 gcagcaagta catatatgac ggtactttta caaagacatt gattcccggt cagggaataa
     3421 ctaccagact tcttcaatta aacgacattg aggtaataag tgaccaagaa ttttagctgc
     3481 ttaaaaatac attaacatct cataccccta aatatttaac agagataacg aagaatgtaa
     3541 tacaagaaaa taatgatata aatcaaacat ggaggtttag aatgcaaaca aaatatttaa
     3601 aaagaattat tgcttttgta attgtattgt ccatggtatt taccacggta gctatggcta
     3661 tgccttacgg acaggcgaaa aagcttatta aaaacaagta cagctgggcg caggttcaaa
     3721 aatatgtaaa gggaaggggc ataatgaagg gatatcccga tggtatgttc cgtgaaaatg
     3781 ataaagttaa gcgtgccgat gccattataa tgattgatag ggcatttaaa ttaagtgctt
     3841 tgatagacat aatcaatagg gattataaag atgtatttaa cgatgttgac aaaaatgaat
     3901 attattttga tgcagtacac atagctagac tattaggaat aacaaagggc aaaggaaata
     3961 ataatttcca ccctaaacaa gccataacta tcagagaagc tatattgtta atagaacgtg
     4021 caatggacag aaacaaatac tttgattttg atgatgacat tgatttatgg aaagaatatg
     4081 aagaaaaaga attagataaa tctgctacca gaggcgatat agccgctatg ctttactatg
     4141 ttctaacagg aagtaaatat aaaggtgttc ctcaagaata tgaccttgaa gaaatatctc
     4201 ttacaataga aaaagatgaa attttaagat ttgatgatga atatggaaaa aaggacagta
     4261 ttattgagtc tattgaagat atggtagatg atttagaata tgttcaattt acaaagccct
     4321 taaataaaga caacaccttc ttatattatg attataacag ccgtagtcgt tctaattcat
     4381 ttgtaaccaa ccgtaataaa tattatattg acccggacga tgagcagccg gatctatcaa
     4441 aagtatcagt tgtgcctaaa acaagcggtg tcttagaaat agaatacata gcttatgatg
     4501 aagatggaga atcatataaa ggtttaatca aaatcactgt tgaaggtaaa tatgatgatt
     4561 tagatgatat tacatttacg ggatatgaga atactcctat aaaattcatg atttcagagt
     4621 ttaaaaaagt gtttaataaa gaatttgaat atacaccgga tgatgaaata agctttgaac
     4681 ttccggatga aaaattcggg accttgtatt ttgatgaaaa caaagacggc gaactaaaag
     4741 atgcggaaga gattgaagca aagaattcat tcaaattaaa acaactgaat tttatttatt
     4801 tcaatcctgc tgaagattat gttgatgaag atgagccggt agaaattaaa tacacggtcg
     4861 aagaccaaga aggcaattta tatgaaggca aaataaatat aacagtaaaa tctctgcttg
     4921 gtgaattgac tattgatgaa gatgatgatt tcggcataga agttaaagac tacttggatg
     4981 aatttgatga ttatgatttt caaaaaatta aatttgaaaa ggcagataaa gatgataagt
     5041 caactctaac aatagaaaca aacacaggaa caaggtcagt aattggaatg gaactaaata
     5101 ttagacggtt acaagatata gaatttgaac ctgaaaaaga ctttgacgga accacattta
     5161 aatacacggt taccacagat gatggagaac aatttatcgg attagttaca gttgaaatgg
     5221 acgattaaat atataatagt taaaggactg agtttgataa ccaagctcaa tcctttttta
     5281 tttttaatta ttgatttaaa tcaatttatt ttttcaaata atttggtaga atatagacat
     5341 cataaaaaag gagaaaagaa ggaggaaata tggcaaaatt taatgaaaca gttgaaaaac
     5401 ttattccgac attggataaa ttcgtaccga ttgtagcaag agttcatgga gggcatcacc
     5461 cggaatttca tgatgttaga aagatatttg acgtaatcaa agaaaaagta aaagaagcgg
     5521 gagcagaaaa acccaacttg agtgaagaat ttgctgaact gcgtgaaata accgataatt
     5581 acaccgttcc cggtgatgta tgcgaaagtt atgaagcagt gtataagatg ttagctgaat
     5641 tagacgaagc gtaccaggca taaacagaag ctgtttttta aaataatcac attttccgtt
     5701 gattgttaac ggcatattgt attatgatat aaatgataat atcagttaa
//
LOCUS       DAOSHP010000100         5745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12352_length_5745_cov_8.733392, whole genome shotgun sequence.
ACCESSION   DAOSHP010000100 DAOSHP010000000
VERSION     DAOSHP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_5745_cov_8.733392"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1240)
                     /locus_tag="PLB33_08260"
     CDS             complement(44..1240)
                     /locus_tag="PLB33_08260"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOG63557.1"
                     /translation="MNVLVINCGSSSLKYQLINMDDESVLAKGLVERIGIDGSLLKHE
                     RTGLDKLVIEEDLKNHKDAVNLMLRALLDEKYGAVKSLDEIDAIGHRVVHGGEKFACS
                     VVINDEVIDTMKENIGLAPLHNPPNIIGIEACKELLPDVPMAGVFDTAFHQTMPPVSF
                     IYPLPYELYEEMRIRRYGFHGTSHKYVSERVAEITGKSLEGTKIVTCHLGNGASLTAI
                     KDGKSFDTSMGMTPLEGLVMGTRCGDIDPAIVTYLICKKDMTAEQVDNLMNKKSGVLG
                     ISGVSNDFRDIEAEADKGNKRARLALDKFAYTVKKYIGSYAAAMGGLDYIVFTAGLGE
                     NSATARKEICEGLEFLGVEIDDEKNKIRGKEAEISKDGSRVKVFVIPTNEELMIARET
                     KELTSK"
     gene            complement(1297..1872)
                     /locus_tag="PLB33_08265"
     CDS             complement(1297..1872)
                     /locus_tag="PLB33_08265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOG63558.1"
                     /translation="MIYLVRHGESESNISRRFSGITDVELTETGALQAVKAGKKLRGK
                     TIDNIFSSPLKRAKNTAEIIADEIGFNKKDIIIENRLTEVNFGIFENLTWEEILEQYA
                     DETERWIEFKHKYKFPKGEGYDDIIYRVSGFIDNVPDNSLILTHYGVIQGILLYYKIV
                     DDNTLWNYQISNCDIFILNNKKLENIIKCVH"
     gene            complement(1869..2606)
                     /gene="cobS"
                     /locus_tag="PLB33_08270"
     CDS             complement(1869..2606)
                     /gene="cobS"
                     /locus_tag="PLB33_08270"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOG63559.1"
                     /translation="MASGFLILISFFTRIPVGEKIEYNEENFVKALNLFTLVGVVIGF
                     LLAVVYLIFNNQISFIRGLVITVMYILISGGIHLDGACDTADGLLSGRTGDRIFEIMS
                     DSHIGAFGVIALILIILSQFVLFSFCSIETVFMMPLVGRASTIVSCWNKKYAKKIKGM
                     GTVFIENIDKNVLLINLLILFVFSMIPWYRAIMFTACFAAIAVSYFISILIEEKIGGM
                     TGDTCGFITEISQIVFMTIVLFLMRQI"
     gene            complement(2588..3181)
                     /gene="cobU"
                     /locus_tag="PLB33_08275"
     CDS             complement(2588..3181)
                     /gene="cobU"
                     /locus_tag="PLB33_08275"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOG63560.1"
                     /translation="MSLIFVIGGARSGKSTFAELKAKEYSENVLYIATAAVTDEAMAD
                     RVKKHREQRPAFWQTLEMYSDFKTLINKAEFNQSEVVLLDCITTMIGNYMVESKLDFD
                     DCSVEDVNILENKITEEVFSLINVCKENNKKLIIVSNETGMGLVPSYYMGNYFRDISG
                     RINNKIGVQAEHMYFIFSGIPIKLKHKGEMIKWPQDF"
     gene            3406..4605
                     /locus_tag="PLB33_08280"
     CDS             3406..4605
                     /locus_tag="PLB33_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOG63561.1"
                     /translation="MRITGVVAEYNPFHSGHEYQLRMARRLSGCDCIAVVMSGNYVQR
                     GEPAIIDKFKRAEAAIYGGADIVIELPMPFSCQNAEMFAHAAIMELKKLNVDSISFGC
                     ETYNVRLLKKIALLQLTPGFNNYIKEEMKKGLSYPNAMSNALKFILNEEAEAVYSPNN
                     VLAVEYIKSAVKLNLELQYYPVKRVGMAHNDTEISGLFHSATAIRNAIMEGSFDNLKL
                     TEKSIELIKKFCKEHKKYNILNNYRDYLYYKIITCDDLEEIYEINEGLNNKIISQVYK
                     HETIDEFIMSLKSKRYTYSKLRRSLLNILLGIKKVDIINIMSTNDKYVKVLAFNNTGR
                     KAIKNAVNNGTIVINRYSDYKKNNLTPEKLMRFNITNKTSNIYYLPLENRKMNSEYFE
                     NAVYVDV"
     gene            4711..5661
                     /locus_tag="PLB33_08285"
     CDS             4711..5661
                     /locus_tag="PLB33_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63562.1"
                     /translation="MLRRNFIPVFIILIICLFVMKPGIVSEGVFNGLKIWVNNMIPYL
                     LPMSILSNILLQYNFLYRISEKLSFLSNKIFNSKYAFIPYFISFVVGYPSGAMTVNTM
                     ASFKRIDSSEANALMVFTNNCSFQFMAGAVSFSMLGDLKLAKYIAIPHLLGALLIGII
                     IKKGSISTAFTKEKKHKYISFYDAFNSSIYKSITSILSIGGVIVIFSVFSNFFDNLLI
                     SVSKYLPLSINLSDIIHSVLTGILEISNGCSVTASSVLPLDVKLIIINFLISFSGISV
                     IFQTIAVANDFDFKISNYVFYRAIQGILSVIICIGMLILS"
BASE COUNT         1870 a         1038 c          832 g         2005 t
ORIGIN      
        1 attatatgag aaaagaaaat atttgtcaag gtaatatttc ttattatttg cttgttaatt
       61 cctttgtttc tcttgcaatc atcaattctt cgtttgtagg aattacaaat acttttactc
      121 tggagccatc ctttgatatt tcggcttctt ttcctcgtat tttgttcttt tcatcatcta
      181 tttcaactcc aagaaattcc aagccttcgc atatctcttt tcttgcagtt gcggaatttt
      241 cacctaaacc cgcagtaaat acgatataat ccaatcctcc cattgctgct gcataagagc
      301 cgatatattt tttaactgta tatgcaaatt tgtctaaggc caacctggct ctcttatttc
      361 ctttatcagc ttcggcctca atatctctaa aatcgtttga aactcctgaa atacccaaaa
      421 ctccggactt tttattcatt aaattgtcaa cctgttcagc cgtcatatct ttcttacaga
      481 ttaaatatgt tactattgca gggtctatgt ctccgcatct cgttcccatt accaaacctt
      541 caagaggagt cattcccata cttgtatcaa atgattttcc atcctttatt gcagttaagc
      601 ttgctccgtt tcccaaatga catgtaacta ttttggttcc ttcaaggctc ttgcctgtta
      661 tttcagcaac tctttcagaa acatatttat gcgatgttcc gtggaatccg tatcttctta
      721 ttctcatttc ttcatataat tcataaggca atggatatat aaaggatact ggaggcattg
      781 tctgatgaaa tgcagtatca aatactcctg ccatcggtac gtcaggcaat aattccttgc
      841 atgcttcaat acctatgatg tttggaggat tgtgaagagg tgccaatcca atattttctt
      901 tcattgtatc aataacttca tcgttaatta ctactgaaca tgcaaatttt tctccgccgt
      961 gaactaccct gtgtccgata gcatcaattt cgtccaatga ttttactgct ccgtactttt
     1021 catctaacag tgccctcaac ataaggttga ctgcatcctt gtgatttttt aaatcttcct
     1081 caataaccag tttgtctaat cctgttcttt catgttttaa taagctgccg tcgattccta
     1141 ttctttcaac taagccttta gctaaaacac tttcatcatc catgtttatc aactggtatt
     1201 ttaatgatga actcccgcaa ttaattacta atacattcat tttattcctc catagtttta
     1261 aaataataat aataatatct aaaatccctt aagattttaa tgaacacatt taataatatt
     1321 ttctagtttt ttgttgttta aaataaatat atcacagttt gatatttgat aattccacag
     1381 tgtattatca tcaactattt tataatacaa tagtatcccc tggattacac cgtaatgagt
     1441 caagattaat gaattgtcag gcacattgtc tataaagcct gaaaccctat aaattatatc
     1501 atcataacct tcacctttgg gaaatttata tttatgttta aattcaatcc atctttctgt
     1561 ttcatctgca tattgctcta atatttcctc ccaggtcaaa ttttcaaaaa tgccaaagtt
     1621 cacttctgtt aaacggtttt caataattat atctttttta ttgaaaccta tttcgtctgc
     1681 tattatttcc gctgtatttt ttgctctttt taaggggctt gaaaaaatat tatctattgt
     1741 tttacctctt aatttcttgc ctgcctttac tgcctgcaaa gcacctgttt cagtcaattc
     1801 aacatctgta ataccgctga atctcctgct tatatttgat tcggattctc cgtgacgaac
     1861 taaatatatc atatctgcct cattaagaaa agtacgattg tcataaagac tatctggctt
     1921 atttctgtga taaatccgca tgtatcccct gtcattccgc ctattttttc ttctatcagt
     1981 atggatatga aatatgaaac agcaattgca gcaaaacatg cagtaaacat tatagcccgg
     2041 taccatggta tcattgaaaa aacaaataat atcaataaat taatcaataa tacattttta
     2101 tcaatatttt ctataaatac agttcccata ccttttattt tcttagcata ttttttattc
     2161 caacaagaaa ctattgtgct tgcccgtcct acaagaggca tcataaaaac agtttcaatg
     2221 ctgcaaaaag aaaacagaac aaattgtgaa agtataatta aaatcaatgc tataacacca
     2281 aaagctccta tgtgggaatc actcattatt tcaaagattc tatcccctgt ccttccggat
     2341 aataagccgt ctgcagtatc acaagcacca tccaaatgta taccgccgct tataagaatg
     2401 tacataacag taattaccag ccctcttata aaggatattt gattgttaaa aattaaataa
     2461 acaacagcca aaagaaaacc tattaccact cctactaaag tgaaaagatt caatgctttt
     2521 acaaagtttt cttcattata ctctattttt tccccaacag gaatccgagt aaaaaaggaa
     2581 atgagaatta gaaatcctga ggccatttta tcatttctcc tttatgttta agtttaattg
     2641 gtattcctga aaaaataaaa tacatatgct cggcttggac tccaatttta ttgttaatcc
     2701 gcccgcttat atccctgaaa taatttccca tgtagtaaga aggtacaaga cccattcctg
     2761 tttcattaga gactattata agttttttat tgttttcctt gcatacatta attaatgaaa
     2821 aaacctcttc ggtaattttg ttttccaata tatttacatc ttcaacactg caatcatcaa
     2881 aatccaattt gctttcaacc atgtaatttc caatcatcgt tgttatgcag tccaaaagaa
     2941 ctacttcaga ctgattgaat tcagctttat taataagtgt tttaaaatca gagtacatct
     3001 ccaatgtctg ccagaaagcc ggtctttgct ccctgtgctt tttaactcta tctgccatag
     3061 cctcgtctgt tacggctgca gttgctatat acaatacgtt ttccgaatat tcttttgctt
     3121 ttaattcagc aaaggtgctc ttcccgcttc ttgcaccgcc gataacaaat ataagactca
     3181 ttctatcacc atttaatatt tttaaccatt aacatttaac agtatatcat ttatttaata
     3241 aaaaataaac aatgaatttt aaaattttaa ggacgggtgt tctcgcggtg cctggcaccg
     3301 ggctgcatta acaaaggacg gttccaaata gaaccgtccc gaaattttat tgacattgat
     3361 attgattata attaagatat atgtaaaggg cgaaaggata aaattatgag aataacaggt
     3421 gttgtggcgg aatataatcc atttcattca ggacatgaat atcagttaag gatggcaaga
     3481 aggctatcag gctgtgattg tattgcagtt gtaatgagcg gaaattatgt acagcgcgga
     3541 gagcctgcaa ttatagataa atttaaacgg gcggaggctg caatttatgg cggtgctgat
     3601 attgttatag agcttcccat gcctttttca tgtcaaaatg ctgaaatgtt tgctcatgca
     3661 gcaataatgg aattaaaaaa gcttaatgtt gattcaataa gctttggctg tgaaacctac
     3721 aatgtaagac tattgaaaaa aattgcttta ttacaattaa cacccggttt taacaattat
     3781 attaaggaag aaatgaaaaa aggattatca tatcccaatg ccatgtctaa tgcacttaaa
     3841 tttatactta atgaagaagc agaagccgta tattcaccta ataatgtctt ggctgtcgaa
     3901 tatataaaaa gtgccgtgaa acttaactta gaacttcaat actatcctgt aaaacgtgtg
     3961 ggaatggctc acaatgacac cgaaatttca gggctctttc acagtgctac cgcaataaga
     4021 aatgccataa tggaaggctc ttttgataat cttaagctta cggaaaaaag tattgaattg
     4081 ataaaaaaat tctgcaagga gcacaaaaaa tataatatat tgaacaatta tagggattat
     4141 ttgtattata aaataattac atgtgacgat ttagaagaaa tttatgaaat aaatgaagga
     4201 ttaaacaata aaataatatc acaggtttac aagcatgaaa ctattgatga atttataatg
     4261 tctcttaagt caaagcgtta tacctattca aagttgagaa gaagccttct gaatatctta
     4321 ctcggcatca aaaaggttga cataattaat attatgtcaa ccaatgataa atatgttaag
     4381 gttcttgctt ttaataatac cggcaggaag gcaataaaaa atgccgtaaa taatggaacc
     4441 atagtaataa acagatattc cgattataaa aagaataatt taactcctga aaaacttatg
     4501 aggtttaata taacaaacaa aacatcaaac atttattatc ttccgttgga aaacagaaaa
     4561 atgaacagcg aatatttcga aaatgcagtc tatgtagatg tataatttta gggggcccgt
     4621 ttatttactg aagaattgtg attgtcataa taattatata ctaagcatat aaagcactct
     4681 tggacaataa aataaaaagg gggtgttttt atgcttagaa gaaattttat tcctgttttc
     4741 ataatcctta ttatttgctt attcgttatg aaaccgggca tagtatcgga aggtgttttc
     4801 aacggtctta aaatatgggt caacaacatg attccgtatt tgcttcccat gtctatttta
     4861 tcaaatattt tgctgcaata taatttttta tatagaatat ctgagaaact ttccttttta
     4921 tccaataaaa tttttaacag caaatatgca ttcatcccct actttataag ctttgtggta
     4981 ggctatcctt caggtgcgat gacagtaaat accatggctt cgtttaaaag aatagacagc
     5041 agtgaagcaa atgctctgat ggttttcaca aacaactgca gctttcaatt tatggcagga
     5101 gcagtatctt tttcaatgct cggcgattta aaactagcca agtatattgc cattcctcat
     5161 ttgttaggtg cattacttat agggattatc ataaaaaaag gcagtatatc tactgctttt
     5221 acaaaggaaa aaaaacataa atacatttca ttttacgatg ctttcaattc ttcaatttat
     5281 aaatcgatta cctcaatttt atccatcggg ggcgtaattg ttatattttc tgtattttcc
     5341 aatttttttg acaacctcct aatatcagtt tcaaaatatc tccctctaag tataaatcta
     5401 agcgatataa tacattctgt tttaacagga atattagaaa tttcaaacgg ctgcagcgtt
     5461 acagcttcct ctgtacttcc cttggacgta aagcttataa taatcaattt tcttatcagc
     5521 ttctcaggaa tatcagtaat ttttcaaacc attgccgttg caaatgattt tgattttaaa
     5581 attagcaatt atgtttttta tagagctata caaggaatat tatcggttat aatatgtatc
     5641 gggatgttaa ttctatcttg aaaagtattg taagtatata tataatggta ttacttaaga
     5701 aaaaggtgat ataatgataa acgttacttt aatcggtaca ggcgg
//
LOCUS       DAOSHP010000101         5702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12475_length_5702_cov_9.331858, whole genome shotgun sequence.
ACCESSION   DAOSHP010000101 DAOSHP010000000
VERSION     DAOSHP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12475_length_5702_cov_9.331858"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1622
                     /locus_tag="PLB33_08290"
     CDS             72..1622
                     /locus_tag="PLB33_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOG63563.1"
                     /translation="MKIKYFPLIIFFIVYILLFATLPQNIKLPLLYFTPLLLYTMFIS
                     KAMFYYMKNKKQDDDMEVNEIASTVTYFDKKKIDEQKIQISFNDVAGLEEIKEDLMDI
                     IDFLNNEDKYRLMDAKVPRGILLYGPPGTGKTLIAKAIAGEAKATFIYSSGSEFVEKY
                     VGVGAKRVRTIFEKARKEAPSIIFIDEVDALGAKRNADSNNEKDQTLNQLLIELDGFN
                     NTSNVIVIAATNRLDLLDEALLRPGRFDRKIYVGNPNFHSRLKILEVHTKNKPLDKKV
                     QLKDIAIKTHGFSGAQLANIANEAALKAIKEKSKKITNENFEFAIEKIAAGLEIKHST
                     VTDKEKRIVAYHEAGHAVAAKILNIDQVQKISIIPRDKALGYVLKFPTEDKFLYTKNE
                     LCNKVIVLLAGRASEEIFIGEISTGASNDIKESTNIIYEIICNYGMGKNTQNTCIDER
                     FIKYYLDNIKDEAQEMTKEAYESAMAIIRENEAVVDKIAQYLLKYETMNAEKLDELMD
                     ETKKTALI"
     gene            complement(1743..2780)
                     /locus_tag="PLB33_08295"
     CDS             complement(1743..2780)
                     /locus_tag="PLB33_08295"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOG63564.1"
                     /translation="MRIGIVGFGGVGRAFLNLIYNKKNKLESEGIYFQVNYIIGRTGG
                     VYNKHGIDLHKFINFLFDEKDITKYCDGGKAEDINFELMLNNKDIDLMVEMTPTNKET
                     GEPGMTHITRCLENNINVVTSNKGPILLAYHKLYNLAKANKVQLGIGCTTGGALPSIN
                     GGFIDLAGADIISIEGVLNGTTNFILKEMEDTGCNYDDALKEAQRLGIAETNPALDVE
                     GFDTASKLLILTNVLMNTEKTMDDIFIEGITKLTPHDINRAKSMNKKYKLVGKTQILD
                     NKIEMEVKLQLLDPSNPLYGVEGKNKAVRYISDTLGELTIMGGASGVTPAAASILRDI
                     ININRGYKFVK"
     gene            complement(2852..3976)
                     /locus_tag="PLB33_08300"
     CDS             complement(2852..3976)
                     /locus_tag="PLB33_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOG63565.1"
                     /translation="MFRKCVAVILLIITIIGIFPANAGGSIADLRYKQKQIEQQIKDI
                     RKKAEELKGEKKSIEAEIQILDSELRALTLETEKQEVQKQELNMRIAESEQKIAELTD
                     EIDKNNMLLEQRLRAMYMNGTGGYLEVILNAENLIDALTRMDLIQLIVKSDVELLKTI
                     NEQKKEVEELKAAQEIRRHELTAVINDLNIKQNEVLIASRSKENYMSSLQNNIEEILR
                     QEAAMEEQSKQIEKDIIALQRAVEYAGGELLWPLPGHYRITSEYGGRIHPITGVWSTH
                     GGTDIAAPYGTNILSSNDGVVIYAGYHYSYGNYIIVDHGGGIATLYAHCSKLLASEGQ
                     AVTKGEPIAKVGSTGESTGNHLHYEVRLNGVRKNPMEYLK"
     gene            complement(3987..4892)
                     /gene="ftsX"
                     /locus_tag="PLB33_08305"
     CDS             complement(3987..4892)
                     /gene="ftsX"
                     /locus_tag="PLB33_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOG63566.1"
                     /translation="MNPKKISYIFRQAFKSMWRNRMMGFASIGSVSAVLIILGFVLIL
                     VLNINNMAMVAKETFDQIAVYIMEGVDDEQIEEMGNSFRKIDGVMGVAFQTKDYALEK
                     VKEDWGEDAILLQDLRHNPFPNTYIVQLSDVSKADKVIAQLQTFEGVDKVRYYQDAVN
                     TLIKLADFVKKFGAGIIIILLLISVFIISNTIKITVLSRQREIELMQYIGATNGYVRG
                     PFILEGVMLGLLGSVLAILLIMLGYNYTIDYLAGRYVALISGMSGYLVGVEMVLYDLI
                     IIFMTIGIGIGVLGSLVSLKKFLSI"
     gene            complement(4873..5568)
                     /gene="ftsE"
                     /locus_tag="PLB33_08310"
     CDS             complement(4873..5568)
                     /gene="ftsE"
                     /locus_tag="PLB33_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOG63567.1"
                     /translation="MIEFNNVTKIYKKTFTAVKNVNLKIDKGEFIFIVGKSGAGKSTL
                     IKLLLREISPSEGIITYNNQDINKIRKRSVAEYRRRIGFIFQDYRLLPKMTVYENIAF
                     AMEMICCQAKNIRREVPLVLSMVGLSDKAKYYPDELSGGEQQRVAIARAIINKPETVI
                     ADEPTGNLDMETSKEIMKILTEINRRGTTVIMATHDREIVNQYQRRVVELKNGIVIRD
                     IKAGGYSYESEED"
BASE COUNT         1776 a         1128 c          875 g         1923 t
ORIGIN      
        1 aacttttttt gtaatggtaa agataaagat atcaataaaa atatttagag ttttcgacat
       61 aaggaggtaa tatgaaaatt aaatatttcc cattaataat tttctttata gtatatattc
      121 ttttatttgc tacactacct caaaacatta aactaccact actatatttt actccgttac
      181 tattatatac aatgtttatt tctaaagcaa tgttttatta tatgaaaaat aagaaacaag
      241 atgatgatat ggaagtaaat gagatagctt ccaccgtaac atattttgat aagaaaaaaa
      301 ttgatgagca aaaaattcaa ataagcttta atgacgtggc aggacttgag gagattaaag
      361 aagatttaat ggatattata gattttttga ataatgaaga taaatacagg ttaatggatg
      421 caaaggttcc aagaggcata ctcttatacg gacctccggg aaccggcaaa acacttattg
      481 ctaaagcaat agcaggagaa gcaaaagcaa cattcattta ttcgagcggc tcggaatttg
      541 tagaaaaata tgtaggtgta ggtgctaaaa gggtgagaac catatttgaa aaagctcgaa
      601 aggaagcacc cagtataatt tttatagatg aagtggatgc tcttggtgcc aaaagaaatg
      661 ccgacagcaa caatgaaaag gatcaaaccc taaaccagct attaattgag cttgacggat
      721 ttaacaacac cagcaatgta attgttattg ctgcaaccaa caggctggac ttattggatg
      781 aagccttgct gaggcccggc agatttgaca gaaaaattta tgtcggaaac cctaattttc
      841 attccagact taaaatcttg gaagttcaca ctaaaaacaa acctcttgac aaaaaggttc
      901 agcttaagga tattgccatt aaaactcacg ggttttccgg agctcagctt gccaacatag
      961 caaatgaagc agcacttaaa gcaataaagg aaaaatcaaa aaaaattacc aatgaaaatt
     1021 ttgaatttgc catcgaaaaa attgccgcgg gtttggaaat aaaacattcc acggtaacag
     1081 acaaggaaaa acgaattgtt gcatatcatg aggcaggtca tgcagttgct gctaaaattc
     1141 ttaatataga ccaagttcag aaaatttcaa taattccaag agacaaagcc ctgggatatg
     1201 ttttaaaatt ccctacggaa gataaattcc tctacacgaa aaacgagctt tgcaacaaag
     1261 taattgtact tcttgcaggc agagcttccg aagaaatatt tatcggtgaa atatcgacgg
     1321 gtgcttcaaa tgatattaaa gagtccacaa atattattta tgaaattatc tgcaattacg
     1381 gcatgggcaa aaatactcaa aacacatgca ttgatgaaag atttattaaa tattatttag
     1441 acaatatcaa ggatgaagct caagaaatga caaaagaagc atatgaaagc gccatggcga
     1501 taattagaga aaatgaagca gttgttgata aaattgctca atacttattg aaatatgaaa
     1561 ccatgaacgc agaaaaatta gacgaattga tggatgaaac caaaaaaaca gctctgattt
     1621 gagatccttc gcttacgctc aggatgacaa acacagagta tactcgatta tcgtggcgtg
     1681 aaagtaggga cggttctttt tgtttcaaga acaaaaagaa ccgtccccag ccaactaaaa
     1741 atctacttta caaacttgta acctctgtta atatttatta tgtctcgcaa tattgaagct
     1801 gctgcaggag taactcccga agctccgccc ataatcgtta attcccctaa tgtgtcggat
     1861 atatatctta ctgctttatt cttaccctca actccgtata aaggattgga ggggtccagc
     1921 aattgcagtt ttacttccat ttctatttta ttatcaagaa tttgtgtttt acctaccagc
     1981 ttatattttt tgttcatgga ttttgctcta tttatatcat ggggtgttaa ttttgtaatg
     2041 ccttctataa atatatcatc catagttttt tccgtattca tcaatacatt tgttaatatt
     2101 aacagttttg aagcagtatc aaatccttca acatcgagag ccggatttgt ttcagctata
     2161 cccagccttt gagcttcctt cagggcatcg tcataattac agcctgtatc ctccatctct
     2221 tttaaaatga agttcgtagt tccgtttaaa actccttcaa tagaaataat atcggctccg
     2281 gccaaatcga taaatcctcc gttaatagaa ggcagtgccc ctcccgttgt acaaccgata
     2341 cccagttgta ccttattagc tttagccaaa ttatataact tatgataagc cagcagtatg
     2401 ggtcctttgt tagatgtcac tacattgata ttgttttcaa gacaccttgt gatatgagtc
     2461 atgccgggct ctccggtctc tttattggta ggtgtcattt caaccattaa gtctatatcc
     2521 ttattattga gcataagctc aaaatttata tcttctgctt ttcccccgtc acaatacttg
     2581 gttatgtctt tttcatcgaa aagaaagtta ataaatttat gtaaatctat gccatgctta
     2641 ttataaactc caccggttct gcctattata taattaacct gaaaataaat tccttcactt
     2701 tccaatttat tttttttgtt ataaattaaa tttaaaaatg ctctgcctac tcctccaaaa
     2761 ccgactattc caattctcat aattactcct tatttagaat atcaaggacg gctcttttcg
     2821 tgtgattgaa aagaaccgtc cctaactatc attactttaa atattccatc gggtttttcc
     2881 tgactccgtt aagtcttact tcgtaatgaa ggtgatttcc cgtagattct cctgttgagc
     2941 caacctttgc aatgggctcg cctttagtta ctgcttgtcc ctctgaagcc aacaactttg
     3001 agcaatgggc atagagtgtt gcaattcctc ccccatggtc aacaattatg taattgccgt
     3061 aagaataatg gtatcctgca tatattacaa ccccgtcatt tgatgataga atattcgttc
     3121 cgtatggagc tgcaatatcc gtacctccat gagttgacca aacccctgtt atcggatgaa
     3181 tccttcctcc atattcggat gtaatcctgt aatgtccggg gagaggccac agtaattcgc
     3241 ctcctgcata ttcaacagca cgctgtaatg ctataatgtc tttttcgatt tgctttgact
     3301 gctcttccat ggcagcttcc tgtcttagaa tttcttcaat attattttgc aggctggaca
     3361 tgtagttttc ttttgaacga gaagctatta atacttcatt ttgcttaata tttaaatcat
     3421 taattactgc agtcagctca tgccttctga tttcctgggc agcttttaat tcctcaactt
     3481 ctttcttttg ttcatttata gtttttagta attcaacatc gcttttaacg attaattgaa
     3541 tcaaatccat tcttgttaat gcatcgataa ggttttcggc attgagaatt acttccagat
     3601 atccgcctgt tccgttcata tacatagctc ttaagcgttg ctccaaaagc atattattct
     3661 tatctatttc atcagtcagc tcagcaattt tttgctctga ttctgctatt cgcatattta
     3721 gctcttgctt ttgaacttct tgtttttcgg tttccagagt taatgctctg agctcactgt
     3781 caagaatttg aatttcagct tcaatacttt tcttttcgcc tttaagttct tctgcctttt
     3841 tacgtatgtc tttgatttgc tgctctattt gtttctgctt ataccttaga tcagcaatac
     3901 ttcctcctgc atttgccgga aaaataccta ttattgttat tattaacaga ataacagcga
     3961 cacattttct aaacataata cactccttaa atgctcaaga attttttcaa tgaaaccaag
     4021 cttcctaata ctccgatacc tattcctatt gtcataaaaa ttatgataag gtcatacagt
     4081 accatttcaa cgccgaccaa gtatcctgac attcccgata taagtgcaac ataacgaccg
     4141 gcgagataat cgatggtata attataacca agcataataa gtaaaattgc caatacagag
     4201 cccaatagtc ccaacatgac accttccagt ataaaaggtc ctctgacata tccgttggta
     4261 gctccgatat attgcataag ttcaatttcc ctttgtctgc ttaaaaccgt aatcttgatt
     4321 gtattggata taataaatac acttattaat aaaagtatta ttattatgcc tgctccaaat
     4381 tttttaacaa aatcagctaa ctttataagg gtgtttacgg catcctgata ataccttacc
     4441 ttatcaacac cttcaaaagt ctgcagttgt gcaattactt tatctgcctt gcttacatcg
     4501 gaaagctgta caatatatgt gtttggaaaa ggattgtgtc ttaaatcctg cagtaaaata
     4561 gcatcttcgc cccaatcttc cttaaccttt tcaagagcat agtcctttgt ttggaatgca
     4621 actcccatta ctccgtcgat ttttctgaag gagttaccca tttcctctat ttgctcatca
     4681 tccacgcctt ccattatata tactgcaatt tggtcgaaag tttccttggc aaccattgcc
     4741 atattattta tatttaatac cagtattaaa acaaatccga gaattatcag tactgccgag
     4801 actgagccta tggaagcaaa acccatcatt cgatttcgcc acatactttt aaatgcctgt
     4861 ctgaatatat agctaatctt cttcggattc atagctgtat ccccctgcct tgatgtctct
     4921 gataacaata ccgtttttaa gctcaactac tcttctttga tactgattta ctatttctct
     4981 gtcgtgtgtt gccataataa ccgtagttcc tcgcctgttt atttccgtca atattttcat
     5041 tatttcctta gatgtttcca tatccaaatt acctgtaggc tcgtctgcaa ttacggtttc
     5101 aggtttatta ataatagctc ttgcaatagc aacacgctgc tgctctcctc ctgacaattc
     5161 atcgggataa tatttggcct tatcgcttaa tcccaccatg ctcaaaacca gtggaacctc
     5221 tcttcgaatg ttttttgcct gacaacaaat catttccatg gcaaatgcaa tattttcata
     5281 tacagtcatc ttaggaagca gcctgtagtc ttgaaaaata aagccgatac gccttctgta
     5341 ttccgccacg cttctttttc tgattttatt aatatcctga ttattatatg taataattcc
     5401 ttccgaagga cttatttccc taagcaataa ttttattaag gtacttttac ctgctccgct
     5461 ttttcctaca atgaagataa attcgccttt atcaattttt aaattgacat tttttacagc
     5521 ggtaaaagtt ttcttatata tctttgtaac attattaaac tcaatcactg tttcacctca
     5581 tagcgtataa tattaatata acacaaaatg acaaaatttc aactattatt ataatatttt
     5641 actttatttt taaaaaaaat gaaatgttta tttttaacat atataaaaat atatgtaatc
     5701 ct
//
LOCUS       DAOSHP010000102         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12676_length_5640_cov_10.206088, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000102 DAOSHP010000000
VERSION     DAOSHP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12676_length_5640_cov_10.206088"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /gene="recR"
                     /locus_tag="PLB33_08315"
     CDS             <1..531
                     /gene="recR"
                     /locus_tag="PLB33_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOG63568.1"
                     /translation="RKSAQRLAFHILEMKSSDVIELAKAIVNAKKYTKHCQRCGNLTE
                     GELCGICRSLKRDSSTICVVEDARDISAMERTKEYKGVYHVLHGTISPLDGIGPDDIN
                     IKSLISRLNTDIKEVILATNPTIEGEATAVYISRLIKPMGIKVTRIAHGIPVGGDIEY
                     ADEVTLMRAMEGRREM"
     gene            636..1685
                     /locus_tag="PLB33_08320"
     CDS             636..1685
                     /locus_tag="PLB33_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOG63569.1"
                     /translation="MNKKDEPESDSKFKSFLRRKNIEISAQRYLIEALGLMAYGLFAS
                     LLIGTILNTIGKKFGISFLTETVWPICSQMTGAAIGVAVAYGLKAPPLVLFASTITGT
                     AGNILGGPAGAFCSALVGAEFGKLVSKETKIDIIITPAVTIIAGVLIASLVGPAIGAF
                     MSATGEFIMYATTLRPFLMGILVSVVVGIVLTLPISSAALCMMLGLSGIAAGAATAGC
                     CAHMVGFAFLSFKENGWGGVAAQGLGTSMLQMPNLVRNPRCWIPAVIVSAITGPMATV
                     LFHMENSPIGAGMGTSGLVGIIETLNTMGGGMKVWLGILILYILIPVVLTPLIGNILR
                     KVGWIKDGDLKLDLV"
     gene            1785..2423
                     /locus_tag="PLB33_08325"
     CDS             1785..2423
                     /locus_tag="PLB33_08325"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOG63570.1"
                     /translation="MAQLYFRYSTMGAGKSIEVQKVAFNYEERGQNPLIFTSSIDNRF
                     AESGIVASRIGLSRRAIPVYDDMNIFEVIKEYNDIFGVDAVIIDECQFMKKHHIQELT
                     DVVDELNIPVMCYGLRADFRNELFEGSYWLFALADKIEEIKTICWCGKKALTNARIVD
                     GKVVYVGEQIFIGGNESYMPLCRKHYKEGKIMPYREKGEDLIIRYEKLKKEK"
     gene            2463..2792
                     /locus_tag="PLB33_08330"
     CDS             2463..2792
                     /locus_tag="PLB33_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOG63571.1"
                     /translation="MAIFEDFMKLDIRVGEIVEAKNFEKARKPAYKLIVDFGHEIGTK
                     KSSAQITECYTVDELIGKQVLAVVNFPPRQIADFISEVLVLGVYTEKGVVLIKPDQRV
                     EKGAKLG"
     gene            2819..3283
                     /locus_tag="PLB33_08335"
     CDS             2819..3283
                     /locus_tag="PLB33_08335"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOG63572.1"
                     /translation="MKSVFYYDTDIGRIGIAEDGNFITNVFFENEELEDIKINETPLI
                     KEAHKQIEEYLRGERKSFDLPISASGTEFQQRVWNALKTIPYGKTVSYKDIAIAIGNE
                     KSCRAVGMANNRNPISIIIPCHRVIGKNGKMVGYGGGIEIKEYLLKLENNNL"
     gene            3369..3614
                     /locus_tag="PLB33_08340"
     CDS             3369..3614
                     /locus_tag="PLB33_08340"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HOG63573.1"
                     /translation="MNDVQRKISIKSIIESFKKNKSADEEMFKNIENAKREWEDAKNI
                     FENVSHPDLVDYAIYKVEAAEQKYIYLLKQFKSNNLT"
     gene            complement(3683..3925)
                     /locus_tag="PLB33_08345"
     CDS             complement(3683..3925)
                     /locus_tag="PLB33_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG63574.1"
                     /translation="MVVREAVINRINEICDDRGIAVNKLANLSGITPSTVYSIFNSGR
                     KDIGIVLIKKICDGFDITLDEFFSSDLFKSLEQELL"
     gene            4167..5219
                     /locus_tag="PLB33_08350"
     CDS             4167..5219
                     /locus_tag="PLB33_08350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOG63575.1"
                     /translation="MPPIAVEQIIKKRDKLYELDYMRFIACFAVMIVHITATGVSEYI
                     HGTFPYILMLTVNRSLKFTTPVFILLSGVTSFYSYSNKNREFKYMEFLKKRLAKVIVA
                     YLIWCIIYYAAYIYFGYYAIDIKFFVKSVLQGTMSYHFYFVIIIIQMYIVGPIFYFIL
                     KNSGKKVTILIFAAVITYLCAEFIRFDLSDRIFLKYIVFYMLGIYIAMEYDNYSLWIN
                     KNKLYIILGYIMMSIIYIVVSYYDSVASTFAWFIFSTMSLFFVYLIGLLMKDKLKNIY
                     GFIEVFGQSSYYVYLMHPLVLTIMILFAEKNFLSVTDKIILNTFTVIPVTVISCLTYT
                     FIKNKFKKKEKTLAVN"
     gene            complement(5245..>5640)
                     /locus_tag="PLB33_08355"
     CDS             complement(5245..>5640)
                     /locus_tag="PLB33_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOG63576.1"
                     /translation="IDFEMYPDEHCVKMQEILSQNGVDEKIIKSAASHGYGLTDVTIK
                     PEHTMEKILFATDELTGLIGASALMRPSKSTLDMELKSLKKKFKSPGFAAGCSREAIK
                     TGAEMLGWELDYLLDETLKAMQEYEKARN"
BASE COUNT         1912 a          808 c         1131 g         1789 t
ORIGIN      
        1 agaaaatctg ctcaacggct tgcatttcat attttggaaa tgaagagcag cgacgttata
       61 gagttggcaa aagcgatagt aaatgcaaaa aaatatacaa aacattgcca aaggtgcgga
      121 aatttaacgg aaggcgaact gtgcggcatt tgccgcagtt taaagaggga cagctcaacc
      181 atatgtgttg ttgaagatgc aagagatatt tcagctatgg agcgaaccaa ggaatacaaa
      241 ggagtctatc atgttcttca tgggacaatt tctcctcttg atggaatcgg tcctgatgat
      301 ataaatatta aaagtttaat atcaaggctt aatacagata taaaagaagt aatcttagca
      361 accaatccca caatagaggg agaagcaacg gctgtttata tatcccgcct cataaaaccc
      421 atgggaataa aggtaacacg catagctcat ggaattccgg taggcggaga catagaatat
      481 gccgatgaag taactcttat gagagcgatg gaaggcagaa gagaaatgta aaaagaattc
      541 cggggacgga cctcaaaatt ccggtaatac tgggatgaag tcaccggaag gtccgttccc
      601 gtaattatgg aataaaaata acaagaggga tgagtatgaa taaaaaagac gaaccggaaa
      661 gcgattccaa atttaaaagt tttctaagaa gaaaaaatat tgaaatttct gctcaaagat
      721 acctgattga agcattgggt ttaatggctt acggtctttt tgcatctttg ctgataggta
      781 caattctcaa tactatcggc aaaaagtttg gcatttcttt tttaaccgag acagtatggc
      841 ctatttgcag tcagatgacg ggagctgcaa taggtgttgc cgtagcttat ggactaaagg
      901 ctccgccttt agtcttgttt gcatctacaa ttacaggaac ggcaggtaat atcttaggtg
      961 ggcctgcagg tgctttttgc agtgctttgg tgggtgctga atttggtaaa ttagtttcaa
     1021 aggaaacaaa aatagatatt attattactc cggctgttac tattatagca ggtgttttaa
     1081 ttgcttcatt ggtaggacct gctataggag catttatgag tgcaacgggt gaatttataa
     1141 tgtatgctac aaccctgagg ccttttttaa tgggtatatt ggtttcagtt gtggtaggca
     1201 tagtattaac actgcctata agcagtgcag ctctttgtat gatgcttggt ctttcaggca
     1261 tagccgccgg cgcagcaact gcaggatgct gtgcacatat ggtaggtttt gcttttttga
     1321 gttttaagga aaacggctgg ggaggagttg cagctcaagg attgggaaca tcaatgctgc
     1381 agatgccgaa tcttgtgagg aatcccaggt gctggattcc tgccgtaata gtatctgcca
     1441 ttacgggtcc catggcaacg gttttattcc atatggaaaa cagtcccata ggtgccggta
     1501 tgggaaccag cggcttggta ggaataattg aaacattaaa taccatgggc ggagggatga
     1561 aggtgtggtt gggaatacta atactataca ttttaattcc ggtggtactc actccgctta
     1621 tcggtaatat tttaagaaag gtcggttgga ttaaagatgg cgatttgaag ttagatttag
     1681 tctagcctaa tgtatgaccc ttaatccgta tattaggttt acataattga ttttatgtaa
     1741 accttagtga taaaagaaag taaaaaataa tatagaggag agttatggca caactttatt
     1801 tcagatacag caccatgggt gctggaaaga gtatagaagt acaaaaggtt gcatttaatt
     1861 atgaagaaag aggacaaaac cccttaatat ttacatcttc aattgataac agatttgcag
     1921 aatcaggaat tgttgcttcg agaatcgggc tgagcaggag agcaatacct gtttatgatg
     1981 atatgaatat ttttgaagtc attaaagagt ataatgatat attcggtgtt gatgccgtaa
     2041 taattgacga atgtcaattt atgaaaaaac accatataca agagcttacg gatgtagttg
     2101 atgaattaaa tatacctgtt atgtgctatg gattgagagc tgatttcaga aatgaattat
     2161 tcgaaggctc ttactggctt tttgcattag ctgataaaat tgaagaaatt aagactattt
     2221 gttggtgcgg caaaaaagca ttaactaatg cccgtattgt ggacggtaaa gtagtatatg
     2281 tgggcgaaca aatttttata ggaggcaatg aaagttacat gcctctttgc aggaagcact
     2341 ataaggaagg aaagattatg ccttacaggg aaaaaggcga ggatttaata ataagatatg
     2401 aaaagcttaa aaaagaaaaa tagatatgat tactcaatag tgattatata aggagatatc
     2461 atatggcaat atttgaagat tttatgaagt tagatatcag ggtgggagaa atagtagaag
     2521 caaaaaactt tgaaaaggca aggaagcctg cttataaact gattgttgat tttggacatg
     2581 aaatcggaac taaaaaatca agcgcccaga ttacagaatg ttatacagtt gatgagctta
     2641 taggcaaaca ggttttggca gttgtgaatt ttcctccgag acaaattgcg gatttcatat
     2701 ctgaagtgct agtgcttggg gtttacacag aaaagggagt agtcttgatt aagcctgacc
     2761 aaagagtaga aaaaggtgcc aaattaggat aaagagaggt ataataagag gaggaattat
     2821 gaaaagtgta ttttattatg atacggatat cggcagaata ggtattgcag aggatgggaa
     2881 tttcataacg aatgtttttt ttgaaaacga ggagcttgag gatattaaaa taaatgaaac
     2941 tcctttaata aaggaagctc ataaacaaat agaagaatat ctgagaggtg aaagaaaatc
     3001 ttttgatttg ccgatatctg catccggtac cgaatttcag caaagggtgt ggaatgccct
     3061 taaaactatt ccttacggaa agaccgtaag ctataaggat attgcaatcg caataggaaa
     3121 tgaaaaatcg tgcagggctg taggaatggc aaacaaccgt aatccaattt caattattat
     3181 tccctgtcat agagtaatag gaaaaaacgg caagatggta ggatacgggg gaggcataga
     3241 aataaaagaa tacctgttaa agcttgaaaa taataattta tagttataca taatgcaaat
     3301 tatttttgga atattaggaa taaaaaggaa cttcctgtct ataataataa ggataggagg
     3361 ttttttccat gaatgatgtt cagagaaaga ttagtattaa aagtataata gagagtttta
     3421 aaaaaaataa atccgcagat gaggaaatgt ttaaaaatat tgaaaatgca aaaagagagt
     3481 gggaagatgc aaaaaacata tttgaaaatg tttcacatcc tgatttagtg gactatgcaa
     3541 tatataaggt tgaagctgca gagcaaaaat atatatatct tttaaaacaa ttcaagagta
     3601 ataatttaac ataagaaggc ggaatattaa ggggggacac ttttaagcgt ccctcttctt
     3661 tctttatctt tcatatcttc ctttataata attcctgctc taaggactta aacaaatctg
     3721 aagaaaagaa ttcatccaat gttatatcaa atccatcaca aatctttttt atcagcacaa
     3781 ttccgatatc tttcctgccg ctgttaaata tactgtagac agtagatggt gtaattcccg
     3841 ataagtttgc aagcttattt acggctatgc ccctgtcatc acaaatttca tttattctgt
     3901 ttattacggc ttctcttaca accatgtcgt cacctccaat gaatacttaa taaaacaatt
     3961 atagcacaat ttcgcaatta cgtatacgca aaaacgtaga tttttaataa aattgcaaat
     4021 aaatttataa aataacgcta ttatgtatct tttgtcatta ataatgaata attatgatat
     4081 atgtgaatat atatatgaat ttctaaaata attgattttc ttctgtataa atgttaaaat
     4141 ggtcaaaata tagaaagagt gatgatatgc cgccaattgc agtcgagcaa attataaaaa
     4201 aaagagacaa gctttatgaa ttggattata tgagatttat tgcatgtttt gcagttatga
     4261 tagtccatat aacagcaacg ggagtttctg aatacattca tgggacattc ccctatattt
     4321 tgatgcttac cgtaaataga agtttaaagt ttactacacc cgtctttatc ctgttaagcg
     4381 gagtgacaag cttttacagc tacagtaata aaaaccgtga atttaagtat atggaatttc
     4441 tcaaaaaacg acttgccaaa gtaattgttg catatttaat atggtgtatt atttattacg
     4501 cggcttatat atattttgga tattatgcga ttgatattaa attttttgtg aaaagtgttt
     4561 tacagggaac aatgtcatat cacttttatt ttgtaataat tattatacaa atgtacattg
     4621 taggtcccat attttatttt attttgaaaa attccggaaa gaaagtgaca atccttatat
     4681 ttgctgcagt aataacatat ttgtgtgctg aattcataag atttgatttg tcggacagga
     4741 tatttttgaa atacattgtg ttttatatgt tgggtatata tattgctatg gaatacgata
     4801 actattcttt atggattaat aaaaataaat tatatattat attagggtat attatgatga
     4861 gtataattta catcgttgtt tcatattatg attctgtggc atctactttt gcttggttta
     4921 tattttcaac tatgtctttg ttttttgtat atttaatagg actattaatg aaggataaat
     4981 taaaaaatat ttacgggttt attgaagtat tcggacaaag ctcttactac gtttatttaa
     5041 tgcatccttt ggttttgact attatgatac tatttgcaga aaaaaacttt ttatctgtta
     5101 ccgataaaat aattctcaat acttttactg ttatacccgt cactgtaatt tcttgtttaa
     5161 catacacctt tataaaaaac aaatttaaga aaaaggaaaa aacactagct gtaaattaaa
     5221 aaggatatgc atggcatatc cgttttaatt tcttgctttt tcgtattcct gcattgcttt
     5281 taacgtttca tccaacaaat agtctaattc ccatcccaac atttcagcac cggtttttat
     5341 tgcttctctg gagcagcctg cagcaaagcc gggtgatttg aattttttct ttaatgactt
     5401 aagctccata tccaatgtac tctttgaagg acgcatcaat gcagaagcac ctatcaatcc
     5461 tgtcagttca tcggttgcaa aaagtatttt ttccatagta tgttcaggtt ttattgtaac
     5521 atctgtaagt ccgtatccat ggcttgcagc tgatttaata attttttcgt ctactccgtt
     5581 ttgggacagt atctcctgca tttttacgca atgttcatca ggatacattt caaagtcaat
//
LOCUS       DAOSHP010000103         5546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_12992_length_5546_cov_10.825168, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000103 DAOSHP010000000
VERSION     DAOSHP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12992_length_5546_cov_10.825168"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..545
                     /locus_tag="PLB33_08360"
     CDS             186..545
                     /locus_tag="PLB33_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:216..218,aa:Sec)
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HOG63577.1"
                     /translation="MKYTFICYSKUGTCKKAKDWLIKNNVDFTERPIKEENPNETELK
                     KWIEKSGFPLKKFFNTSGILYKDLNIKEKLAYMSEEEQIRLLSTDGMLVKRPLLIGED
                     VVLVGFKENEWEKISKN"
     gene            complement(561..1577)
                     /locus_tag="PLB33_08365"
     CDS             complement(561..1577)
                     /locus_tag="PLB33_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HOG63578.1"
                     /translation="MQEKINRVAPGILLSASVAVIAMFLSSLIPGDIIGATVMALLVG
                     MALNPLFSKYEQFNKGVSFTGKMILRLGIILMGVNMNFAEVLDVGKYSLFVMIFTMGT
                     AFGAGNLVGKLFGMNWKLTNLLSVSTAICGGSAVAAVGPVIKAKDEDVAYAISSTFIF
                     DVLTVVVIPWIGMALGMSNMGYGLWVGTAVNDTSSVVAAGYAFSEFAGNTAVIVKLTR
                     TLFIIPYVLIFSFITERIEAKTVSGQVHKPVNIKKIFPYFIIMFLVVVLFRSTGIIPE
                     TIVPGLSKSSKFFMVMALSAIGLKTSYSDIKNIGYKPMILGFIVDTLVVFVSIGIQVA
                     TGRF"
     gene            1688..2587
                     /locus_tag="PLB33_08370"
     CDS             1688..2587
                     /locus_tag="PLB33_08370"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOG63579.1"
                     /translation="MLDNKLQTFLTLCETQNYSQAAKKLNITQPAVSQHIQYLENYYG
                     VLLISNKGKNFELTDEGKALREYGGTLKANSERILPLLQSIKNKSKPLNFGATLTIGE
                     YTVPPILSQIFKEDPEINISMVIENTVVLQKMLWEGKIDFALLEGHFNQEQFEFKLFS
                     NETFIGVCSPENEIANKVYDLEELLEQNLILREIGSGTRDIFEQALYNQNLSIMSFKR
                     RIEINSMNAIIELCHQNIGITFMYREAAKKEISLGYLKEIPIRNFNISHPFCFVYLKN
                     NPNKSQLEFWFEKFILLRNPLGQ"
     gene            complement(2747..3469)
                     /gene="uppS"
                     /locus_tag="PLB33_08375"
     CDS             complement(2747..3469)
                     /gene="uppS"
                     /locus_tag="PLB33_08375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOG63580.1"
                     /translation="MRIPRHIGLIPDGNRRWAQDKGLQKQDGYDFGLDPGLAALKLSV
                     EYGIDEITYYGFTTDNCKRPKIQIESFVKACVDAVNIIAKENVSLLVLGNTKSPMFPK
                     ELLKYTKRTNIGNGGIKVNFLVNYGWDWDISNINVTSSNRKTIMDCLHSKDISRIDLI
                     IRWGGRKRLSGFLPVQSVYSDIYTIDDLWPDFTPEQFHNAITDSCRYNLFIQTFIQLM
                     ICGLISLLNNFIMQLNGTTIRM"
     gene            3707..4102
                     /gene="tsaA"
                     /locus_tag="PLB33_08380"
     CDS             3707..4102
                     /gene="tsaA"
                     /locus_tag="PLB33_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOG63581.1"
                     /translation="MEIKMKPIGYIRTNYKYDSEIPRQSINNMNEKGTIEILDEFKDG
                     IKDIEIGSYVIVLFYFHKSDYWHLISEKRDNKGVYSTRSPRRPNGIGMSIVKIINSEN
                     STLEFLGVDMIDGSPVLDIKPYMDFSPII"
     gene            4343..5290
                     /gene="arcC"
                     /locus_tag="PLB33_08385"
     CDS             4343..5290
                     /gene="arcC"
                     /locus_tag="PLB33_08385"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOG63582.1"
                     /translation="MNRIPRVVIALGGNALEDKSMPSTAEAQLEVVKKTSEYIADISC
                     SNYEIAIVHGNGPQVGRILLASEAAKDITPAMPFDVCGAMSQGYIGYHIQQSMNLALA
                     KRNRNVQVATVVTQVVVDKDDPAFQNPTKPIGPFYSEEEAKKLAEEKGYTVKEDAGRG
                     WRRVVASPIPKRIVEIDTIKNLWDTTITVACGGGGIPVIENEDGSLKGVEAVIDKDFA
                     AERLAEDMDSDILLILTEVEKVSINYKKPNQEDLSVLTLEQAEKYIAEGHFAPGSMLP
                     KVMAAVKFAGSRPGRKAIITSLYKALDALEGATGTVIQG"
BASE COUNT         1996 a          884 c         1025 g         1641 t
ORIGIN      
        1 aagagttttt gcattggcac aataataatg tttatttagg gtaaatatta ttatagagga
       61 atatgacatt agatgatgct caagatgatt tagttagttt attatctcaa agtaagacag
      121 aaaaacagct tatgaaggaa aataaaatag attgattata aaataatgaa acttaggagg
      181 acgaaatgaa atatacgttt atttgttatt caaaatgagg cacatgcaaa aaggctaagg
      241 attggctgat aaagaataat gtagatttta cagaacgacc tataaaggaa gagaatccca
      301 atgaaactga gctcaagaag tggattgaaa aaagcggatt tcccttaaag aagtttttta
      361 atacaagcgg tattttgtat aaggatttaa atataaaaga aaaattagca tatatgtctg
      421 aagaagaaca aatcagactt ttatcaacag acggtatgct tgttaaaaga cctttactca
      481 ttggagagga tgttgtttta gttggattta aggaaaatga gtgggagaaa ataagtaaaa
      541 attaaggcag gttttgaaaa tcaaaacctg cctgttgcta cctgtatgcc tatggatacg
      601 aaaaccacca atgtatcaac aataaatcca agaatcattg gtttatatcc aatatttttt
      661 atatcagagt agcttgtttt taaaccgata gcggacaatg ccataaccat gaagaatttt
      721 gatgattttg acagccccgg cactattgtc tcagggatta taccggtgct gcgaaacaac
      781 acaacaacaa gaaacattat aataaagtaa gggaaaattt tcttgatgtt aacaggttta
      841 tgtacttgtc ctgaaactgt ttttgcctca attctttctg ttataaaaga gaatattaat
      901 acgtagggaa taataaacag tgttcttgta agttttacta ttacggctgt atttcctgca
      961 aattctgaaa aagcatatcc tgccgctaca actgacgagg tatcattaac agccgttcct
     1021 acccacaacc catatcccat attagacatt ccaagagcca tgccaatcca tggtattact
     1081 acaactgtta gaacatcgaa aataaaggtt gacgaaattg catatgctac atcctcatct
     1141 ttggctttaa taaccggtcc aaccgcagca acagcagagc cgccgcatat agctgtagaa
     1201 actgacaaaa gatttgtaag cttccagttc attccaaaga gttttccgac taaattccct
     1261 gcaccaaaag cagttcccat agtaaaaatc ataacaaaaa gtgagtattt gccaacatct
     1321 aaaacctctg caaagttcat attgacaccc ataagtatta ttcctaaacg aagaatcatt
     1381 ttccccgtaa agcttactcc tttgttaaat tgctcatatt tactaaataa agggttcagt
     1441 gccataccta ccaatagtgc cataacagta gctcctatga tgtcgccggg aattaagctg
     1501 cttaaaaaca ttgctataac ggcaacactt gcactcagta aaattccagg tgccacccta
     1561 tttatttttt cttgcataaa aatacctcca cttaatttta aataatatat tgattatatc
     1621 attgaaatct tataaaatat aattataata tattatagtc atataagcta aacttatgga
     1681 ggcaaacatg ttagataata agttgcagac atttctcaca ttatgtgaaa ctcaaaatta
     1741 ttcgcaagcg gctaaaaagc taaatataac acaaccggct gtttcacagc atatacagta
     1801 tcttgagaat tattacgggg tattattaat atctaataaa gggaaaaatt ttgaacttac
     1861 cgacgaaggc aaagctttgc gtgaatatgg tggaacactt aaggcgaact ctgagaggat
     1921 tttaccttta ttacaaagca ttaaaaataa atcaaaaccg ttaaactttg gagcaacttt
     1981 gaccatagga gaatatacgg ttccgcctat attatcacaa atattcaaag aagatcctga
     2041 aattaatatt tccatggtta tagaaaatac agtagtgctc caaaagatgc tgtgggaagg
     2101 gaaaatagac tttgctttat tggaagggca ttttaatcag gaacaatttg aatttaaact
     2161 cttttccaat gaaacattta ttggggtatg ttctcccgaa aatgagattg caaataaggt
     2221 ttatgactta gaggaattgc ttgagcaaaa tctcatactt cgggagatag gaagcggtac
     2281 ccgtgatata ttcgaacaag cactgtataa tcaaaattta agcatcatga gttttaaaag
     2341 acgaattgaa ataaacagta tgaatgctat aatagagcta tgtcatcaaa atatcggaat
     2401 tacatttatg tacagagaag ctgcaaaaaa agaaatttct ttaggttatc tcaaagaaat
     2461 tccaataaga aattttaata taagtcatcc tttttgtttt gtttatttaa aaaataatcc
     2521 caataaatca caattagagt tttggtttga aaaattcata ttattacgca acccattggg
     2581 gcagtaggtc aatcgcaatg agttcccaat ttatgctacg tcaggagcaa ataaattagt
     2641 gaacgagact tcggacgtat catcactgtc ccattaatta tatttaaaac aggcgtatat
     2701 cagtgaatgg gattgatttt atataacccg gcctaccccc ctaatgttac atcctgattg
     2761 tcgtaccatt taattgcatt atgaaattgt tcaggagtga aatcaggcca caaatcatca
     2821 attgtataaa tgtctgaata aacagattgt accggcaaga atccgtaatt gcattatgaa
     2881 attgttcagg agtgaaatca ggccacaaat catcaattgt ataaatgtct gaataaacag
     2941 attgtaccgg caagaatccg gacagtcttt ttcttcctcc ccatcgtatt attaaatcaa
     3001 ttcttgatat atccttggaa tgaagacaat ccataatggt ttttcggtta gagctagtta
     3061 cattaatgtt tgatatatcc caatcccatc cgtaattaac aaggaagtta acctttatgc
     3121 caccatttcc tatattagtt cttttagtat attttaataa ttcttttgga aacattggtg
     3181 atttagtatt gccaagaaca agcagcgaaa cattttcttt tgcaattata ttgacggcat
     3241 ctacacaagc cttaacaaat gattcaattt gaatttttgg cctcttgcag ttatcagtag
     3301 taaaaccgta atatgtaatt tcatcaattc catattctac agataatttt aaagctgcaa
     3361 gtccggggtc aagcccaaaa tcataaccat cctgcttttg cagtccttta tcttgagccc
     3421 atcttctgtt tccatcaggt ataagcccta tatgtcttgg tattcgcata tacacacacc
     3481 tttcttatga tgatggtatt atttgcaata atttcacaat tattctgcac ataatatttg
     3541 ctaataacag accgttttat agtacactac acatcatgat gtataataag tgtcgaataa
     3601 acaaaaattt cattatgttg aaagatgtaa caatttggta tataattaat gcaaatgttt
     3661 accgaccttt aagaaaaaag gattggaaaa tttgagggac aataaaatgg aaataaaaat
     3721 gaagccaatc ggctatatca ggacaaatta caaatatgat tctgaaatcc cgagacaatc
     3781 aatcaataat atgaatgaaa aaggtaccat tgaaatattg gatgaattta aagacgggat
     3841 caaggatatt gaaataggtt catatgtaat tgtcttgttt tattttcata aatctgatta
     3901 ctggcatttg atttctgaaa aacgggataa taagggtgtc tattcaacaa ggtcacctcg
     3961 cagacctaat ggcataggaa tgtccattgt gaagataatt aattcggaaa attccacatt
     4021 ggaatttctt ggtgtagata tgatagacgg cagtccggtt ttggatatta agccatatat
     4081 ggactttagt ccaataatat agtaaatatg aacaattact gggtaaggag gggtgaaatt
     4141 atgtgcgaat tagtgaataa ggtgtttggt ggttgaaagg tttattgggc atggacttta
     4201 atgaggattg cgaatcaaaa attaataaat aatacttgaa ttaatattat atcatttata
     4261 taatatattt gcttacaaaa aacaagataa aaccgcaaca gtatcacaag aaattaaaaa
     4321 attaaatcat aagaggtaaa taatgaacag gataccaaga gtagtaattg cattgggagg
     4381 caatgcgtta gaagataaga gcatgccctc aacagcagaa gcccagttgg aagtagttaa
     4441 gaaaacaagt gaatatatag ctgacattag ttgctcaaat tatgaaatag cgattgtgca
     4501 tggaaatgga ccacaggttg gaagaatatt gttagcttca gaagctgcca aggatataac
     4561 ccctgcaatg ccttttgacg tatgcggggc aatgagccag ggctatatcg gttatcacat
     4621 acagcagagt atgaatttag cacttgcaaa aagaaacagg aatgtacagg tggcaacagt
     4681 ggtaacacag gtggttgttg ataaggatga tccggcattt caaaatccta ccaaacccat
     4741 aggacctttc tattctgaag aagaagctaa aaaacttgct gaagaaaaag gatatacagt
     4801 taaagaagat gcaggaagag gatggagaag agttgtagca tcaccgatac caaagaggat
     4861 agtcgaaatt gataccatta agaacttatg ggatactacc ataacagtag cctgcggcgg
     4921 cggaggaatt cctgtaatcg agaacgaaga cgggtccctt aagggggttg aagctgttat
     4981 cgacaaggat ttcgctgctg aaagacttgc agaggacatg gacagcgata ttcttctgat
     5041 actgacggag gttgaaaagg tgtccataaa ctacaagaag ccaaaccagg aagacttatc
     5101 ggtattgact ttggaacagg ctgaaaaata catagcagaa gggcactttg ctccaggcag
     5161 catgcttcca aaggtgatgg cggcagtgaa atttgcaggt tcaagaccgg gcagaaaagc
     5221 aattatcaca tccctttaca aggcacttga tgcactggaa ggagcaaccg gaacagtgat
     5281 acaaggatag aaaagaatat gaaatggctg ttgctttatg atggtttcca ttaccggggc
     5341 accggccatt tttgattaat ttgggagtta aaaatgtata attatgagtc tgtattaaat
     5401 ggcattgaag atgatattaa ggatattttg ttttcatata taaaaactga aagttataca
     5461 agtaccgaaa aagaaagaga agctgaaaat ttttttatgg actatttttc atctttaact
     5521 tattttaagg aaaacaaaca ctcata
//
LOCUS       DAOSHP010000104         5496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_13123_length_5496_cov_6.695277, whole genome shotgun sequence.
ACCESSION   DAOSHP010000104 DAOSHP010000000
VERSION     DAOSHP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13123_length_5496_cov_6.695277"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLB33_08390"
     CDS             complement(<1..190)
                     /locus_tag="PLB33_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63583.1"
                     /translation="MVKNNVKFKPIVLIFIVAIIGFFVSGIPFRVFAKLSVGDEYKNI
                     YAEYENMNQDINLKSLYEE"
     gene            complement(365..1795)
                     /locus_tag="PLB33_08395"
     CDS             complement(365..1795)
                     /locus_tag="PLB33_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63584.1"
                     /translation="MAVILSIDIDNNIKIIEASKKGETLSVLRCMTIESSGAKDGKII
                     DMDGIVNVINEEFKNNGIKTRKAIFVINSSKIMIRKIKLPLLKKSPEILSMLQIELQQ
                     IVSVDLSKYKIVYEISNATNENRIPYAEYIVYCVPVILVSECVELAAKLKLKLLKIDI
                     SHNCINSLYKNNIKINDVGLNNGENAAFISVNKNFISFSTAHNGFCDFHISSDLEEIV
                     AQLTKFMRYYYSVSGNRSIEKIYIYGNNNHMLKEVIKTKLDLNSEIIESISGLSAETL
                     PDGFELSKYLNNVLTLFSNNKEQALNKSCSSKLKNNYGYAAILLVIAAASTVMMGFFN
                     SRAAMKNELTAMNAFIHDGKNNEIYSVIEEIKNETDYLKFYLQQAQELKAAAAANDYV
                     DTNILREVYRIKPFETRVTSIYSGRDSTQLLCLSPSMSEAALFFSDLKEIEQVKSAYM
                     PAVQSKSGEPFSYSIVLKLKEVSSEE"
     gene            complement(1800..2216)
                     /locus_tag="PLB33_08400"
     CDS             complement(1800..2216)
                     /locus_tag="PLB33_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG63585.1"
                     /translation="MLSAYPACIYKEKDGGYSVIFPDLNHLATCGETLDEALAMAVDC
                     LAGYLFEAEKEGTTVAPPSNMNDIDISAEYDNYVQAFVNIITVDVEEYAKKHFERSIK
                     KTLTIPLWLNNMAVSRDINFSQVLQSALKEKLNIRE"
     gene            complement(2258..2443)
                     /locus_tag="PLB33_08405"
     CDS             complement(2258..2443)
                     /locus_tag="PLB33_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOG63586.1"
                     /translation="MTKFRDLEKLILTDGWRLKSCVGSHFQYVHPIKPGKVTIPSHTG
                     DIPKGVVNSVLKQAGLK"
     gene            complement(2499..3236)
                     /locus_tag="PLB33_08410"
     CDS             complement(2499..3236)
                     /locus_tag="PLB33_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOG63587.1"
                     /translation="MIIVFFITGLLIGSFINVCIYRIPLHKSIIKPPSSCAACGHRLN
                     YYDMLPVANYVINSGKCRYCGERYSIEYPLIELFNGLFYGAIAVKYGFGLALVLYCIL
                     GSILIAVSVIDLKHKIIPDSLNISVAAIGIVFVLYDGSIILDRVFGAFIGFALFFAIA
                     LATDAMGGGDIKLIAGLGLVFGVQGVLFIMFFSFVIGAVISVILLITKFKSRKDEIPF
                     GPFISTAALLYIFYGSEIINLYLSAVL"
     gene            complement(3309..3737)
                     /locus_tag="PLB33_08415"
     CDS             complement(3309..3737)
                     /locus_tag="PLB33_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOG63588.1"
                     /translation="MGRSINKLRSKKGFTLIELIVVLAVLAIIMAIAVPRFVGVQEEA
                     KVESDRATLANIVKISEFYLVKGEMVKGVVGTPQEYTSETTPSLESIIGEDFPAIEFQ
                     SEGNEDAVLADVTVSITDLGVVSAEVGVEGEGTTLVKYPE"
     gene            complement(3819..5021)
                     /locus_tag="PLB33_08420"
     CDS             complement(3819..5021)
                     /locus_tag="PLB33_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG63589.1"
                     /translation="MVSYKCNVIDISGKKRNISRAGDSKEDIIDFLKQQKFTVIEIKH
                     KQTVDLNRFTSKKIKSKDLAVFCKQMHAMLKAGVNIVNILDILKQQTENRKLRSYISL
                     MHEDLQKGYTFSEALRHQKVTFPEIFISMIEAGELSGNIDVIMDRLSKHYEKEYKIEN
                     KIKSAMTYPIILSIVCTLVVIFLLTSIMPTFIEMYVSSGVSLPKLTMIIINFSNLLKQ
                     SWFLFVLGIAVFIFAISFAGKIPQVKEKRDYIKTRIPLVKYLVIKVAASRFSRTLSTL
                     LGSGAALLNALETVSGVTGNLYISNKILEIREDVRRGLPLSLLLQNQGTFPPMVYYMI
                     KIGEDSGSMEEVLDKTADFYDEEIETAIQRLTTLLEPLMIVVMAIVIGFIVISMVLPM
                     FEMVKTVQ"
     gene            complement(5152..>5496)
                     /locus_tag="PLB33_08425"
     CDS             complement(5152..>5496)
                     /locus_tag="PLB33_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HOG63590.1"
                     /translation="QRLVKKVCLNCREEYAANKAEKRILGINSDVTLQRGRGCPKCYY
                     TGYSGRIAIHEILKIDRVIRDAIQNENSNDEIAVLAEKAGMKTLKDNCRDLVLSGVTT
                     IEEFSRVVYEMP"
BASE COUNT         1681 a         1206 c          765 g         1844 t
ORIGIN      
        1 tttcttcata caagcttttt agatttatgt cctggttcat attttcatat tcagcataaa
       61 tatttttgta ttcatccccc actgatagct ttgcaaatac cctgaaaggt attccggaca
      121 caaaaaaacc tattattgca actatgaata ttaaaactat cggtttgaat ttaacattat
      181 ttttcaccac tgcccacttc cttcagtatt aaattaatta taaaaattac cgcccaattc
      241 atcccttcgg tagcaaacta aatataagaa ttacctctca catctttcag tttcaataca
      301 accctagtgc ttactttctt cggtttcaat actaataatc agaatcaaca gtttgaatat
      361 aaaattattc ttcactgctc acttccttca atttcaacac tattgaatag gaaaatggct
      421 cgccggattt tgactgaacc gccggcatat aagcactctt aacctgttca atttccttca
      481 aatcagaaaa aaataaggca gcttcactca tggaaggaga aaggcacaaa agctgtgtgc
      541 tgtccctgcc cgaataaatt gaagttaccc tggtttcaaa aggttttatc ctgtaaactt
      601 ctcttaaaat atttgtatca acataatcat ttgctgccgc tgccgcctta agctcttgag
      661 cttgctgtaa ataaaatttc aaataatccg tttcattttt tatttcttca attacactgt
      721 aaatttcatt gtttttcccg tcatgaataa aagcattcat tgctgtcagt tcatttttca
      781 tggcagcccg ggaattgaaa aaccccatca tcaccgtgga tgctgcggca attaccaata
      841 aaatggcagc ataaccatag ttatttttaa gctttgagct gcagctttta ttcagtgcct
      901 gctctttgtt attagaaaac aaagttaaaa cattgtttaa gtatttgctc aactcaaaac
      961 catcaggaag cgtttctgca gataaacccg aaatgctttc aataatctca ctgtttaaat
     1021 ccagtttggt tttgattact tctttgagca tatgattgtt attgccgtaa atataaattt
     1081 tttctataga cctgttgccg cttacagaat aataataccg cataaacttg gttagttgtg
     1141 cgacaatttc ctctagatca gaactaatat gaaaatcaca aaatccatta tgtgcagtcg
     1201 aaaaggaaat gaagttttta ttcacactaa taaatgccgc attttcacca ttgtttaaac
     1261 caacatcatt aatcttaata ttatttttat acaatgaatt gatacaatta tgggaaatat
     1321 caattttcaa tagtttaagc ttcagctttg cagccagctc aacacactca ctgactaaaa
     1381 tcaccggaac gcaatacaca atatactcgg catatggtat tctattctca tttgtagcat
     1441 tggatatttc atacactatt ttgtacttgc ttaaatcgac cgaaacaatt tgctgcagtt
     1501 ctatttgaag cattgataaa atttccgggc tcttctttaa taaaggcagc ttaatttttc
     1561 taatcataat tttgcttgaa ttaattacaa aaattgcctt ccttgttttt attccattat
     1621 tcttaaattc ctcattaatt acattgacaa tcccatccat gtcaataatt ttaccatcct
     1681 tggcaccaga actctcaata gtcatacacc ttagaacaga cagtgtttca cctttttttg
     1741 aagcctcaat aatttttata ttattatcta tatcaataga caaaatcact gccataatat
     1801 cactccctga tattcagctt ttcctttaat gccgattgta aaacctggga aaaattaata
     1861 tccctgctta cagccatatt gtttagccac aatggtattg taagtgtttt tttaattgac
     1921 ctttcaaaat gcttctttgc atactcttct acatcaacag taataatatt aacaaatgct
     1981 tgaacataat tgtcatattc tgccgatatg tctatatcgt tcatatttga tggaggagcc
     2041 actgtagtac cttccttttc agcttcaaac aaataacctg caagacaatc cacagccata
     2101 gccaaagctt catccaaagt ttcaccgcag gtagccaaat ggtttaagtc tggaaaaata
     2161 accgaatacc ctccatcctt ttccttataa atacacgccg gatatgcaga caacataata
     2221 atttaccttt ccttttttta atagtatgac atacctctca tttcaatcct gcctgtttca
     2281 gcacagagtt gacaactcct tttggtatat cacccgtatg gcttggaata gttactttcc
     2341 cgggttttat aggatgtaca tattgaaaat gtgatcccac acaagatttt agcctccacc
     2401 cgtcagttaa tattagcttt tctaaatctc taaatttcgt aataaccact ccaatactat
     2461 tatattatcc aatacgtatt atgtcaatac gtattaaatt ataaaactgc cgataggtat
     2521 aaattaatta tttcagagcc atagaatatg tatagtaatg ctgctgttga aataaatggt
     2581 ccaaagggga tttcatcttt tctgctttta aattttgtaa tcaataaaat aactgaaatt
     2641 acagcaccaa ttacaaatga gaaaaacata ataaacaaaa ccccttgaac accaaataca
     2701 agacccaatc cggctataag ctttatgtct cctccgccca tggcatcagt tgccaatgca
     2761 atggcgaaaa acaaagcaaa tcctataaat gcaccaaata ctcggtctaa tataatcgaa
     2821 ccgtcataca atacaaacac aataccaata gcagctactg aaatatttaa actgtcagga
     2881 attattttat gctttaggtc aataacactg actgcaatga gtatgctccc taatatgcag
     2941 tacaaaacaa gagctaaacc aaaaccatat tttacagcga ttgctccata aaacaacccg
     3001 ttaaataatt caatcaaagg atactcaatt gaatacctct caccgcaata cctgcacttg
     3061 ccgctgttta ttacatagtt agctacggga agcatgtcat aatagtttag tctgtgcccg
     3121 caggccgcgc aggaggatgg cggctttatt atggacttat gtaatggtat gcggtagata
     3181 catacattta taaatgaacc tatcaataaa ccggttataa aaaatactat tatcatagat
     3241 tgtccttatt tttcctattg ggacattcct ttgtatcccc ttgatattta tggtgtaggc
     3301 accacgattt attcaggata ctttacaagt gtagtgcctt ctccttctac accaacttct
     3361 gcagatacta ctcctaaatc tgttatactt acagttacat cagctagaac agcatcctcg
     3421 ttcccctcag actgaaactc aatagcagga aaatcttctc caattattga ttctaatgat
     3481 ggagtagttt cacttgtata ttcctgtggt gtccctacaa ctccttttac catttcaccc
     3541 ttcaccaaat aaaactccga tatcttaaca atattagcca aagttgccct gtcagattcc
     3601 acttttgctt cctcctgcac ccccacaaac ctcggcactg caatagccat aattatggca
     3661 agcaccgcca acaccacaat caattcaatc aacgtaaacc cctttttgct gcgcagctta
     3721 ttaatgcttc ttcccatctt tttactctcc ttttcttttt attaataaca ttcaagagat
     3781 tagcaagatg caaacattat gtttcttgaa ttttattttc attgtacagt tttcaccatt
     3841 tcaaacatgg gcaggaccat tgatattaca ataaacccta ttacaattgc cataaccact
     3901 atcattaatg gctctagcaa ggttgttaat ctttgaattg cagtttcaat ctcttcatca
     3961 taaaaatccg cagtcttgtc caatacctct tccattgagc cggaatcctc tccgatttta
     4021 atcatgtaat aaaccatagg cggaaaggtt ccttggtttt gcagcaatag ggaaagcggc
     4081 aatcctcttc ttacatcttc tctaatttct aaaattttgt tgctgatata taaatttccc
     4141 gttacccctg atacggtttc caatgcatta agaagggctg caccgcttcc cagcagagtt
     4201 gacaaggttc ttgaaaatct tgaagcagca accttgataa ccagatactt aaccagcgga
     4261 atcctcgttt taatatagtc tcttttttcc tttacctgcg gtatttttcc tgcaaaagaa
     4321 attgcaaata taaaaacagc aatccccaaa acaaacaaaa accaggattg ctttaataaa
     4381 ttgctgaaat taattattat cattgtcagt tttggaagtg aaactcctga actaacgtac
     4441 atttctatga atgtaggcat gattgacgtt aggagaaata ttaccacaag ggtgcagaca
     4501 attgaaagta tgatgggata agtcattgcg gattttattt tgttctcgat tttatactct
     4561 ttttcataat gctttgagag cctgtccatg attacatcaa tattgccgct cagttctccg
     4621 gcttcaatca tacttataaa aatctccgga aatgtaactt tttgatgccg caaagcttct
     4681 gaaaatgtat atcctttctg caggtcttca tgcattaaac tgatgtaact tcttaacttc
     4741 cgattttcag tctgctgctt taaaatatcc aaaatgttga caatattaac tcccgccttt
     4801 agcattgcat gcatctgctt gcaaaataca gccaaatcct tagatttaat tttcttcgaa
     4861 gtaaatctat tcaggtctac agtttgttta tgtttaattt caatgacagt aaacttctgc
     4921 tgcttcaaga aatcaattat atcctcttta gaatccccgg ctcgagaaat atttcgcttc
     4981 tttccggata tatctataac attgcatttg tacgaaacca tttgttcaac ctccttatcc
     5041 ccctcgcagt ctcgttgcac aattttgttt gctcctatcg tcgcacaaaa ttggcactcg
     5101 ttgcgaatga cctaccgcta tttttctctc cctttggtcg aaaaaatagg gttagggcat
     5161 ctcatatacc acccgggaga attcctcaat tgtcgtaacc cctgacaata ccaaatccct
     5221 gcagttgtcc ttcagtgttt tcatccctgc cttttcagcc aaaaccgcta tctcgtcgtt
     5281 gctgttctca ttttgaatgg catcccttat aaccctgtca atttttaata tttcatgtat
     5341 ggcgattctc ccgctgtagc cggtgtaata acacttcgga caccccctgc ctctttgcag
     5401 ggtaacatca gaattaattc ccagtattct tttctcggct ttatttgcag catactcttc
     5461 cctgcagttt aaacatacct ttttaaccag tctttg
//
LOCUS       DAOSHP010000105         5437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_13304_length_5437_cov_8.294686, whole genome shotgun sequence.
ACCESSION   DAOSHP010000105 DAOSHP010000000
VERSION     DAOSHP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13304_length_5437_cov_8.294686"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1052)
                     /locus_tag="PLB33_08430"
     CDS             complement(294..1052)
                     /locus_tag="PLB33_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOG63591.1"
                     /translation="MEGIQKVDLTKIKPYGDTLNDGMVQMSFTLPVPYGDEANEAAKQ
                     LVSKMGFENPAVVFSKDLGVGYTYFIMYGKCTHTVDYTSIQVPKADIEFMQREEVEEY
                     IKENIKRDVVIVGACTGTDAHTVGIDAIMNMKGFDGNYGLERYKGIEAINMGSQVPNE
                     ELIARAIEVKADAILVSQVVTQKNIHISNLTQLVEMVEAEGLRDKVILVCGGPRINHE
                     LAQELGYDAGFGTGSYANHVASFVVKQIVQRNLI"
     gene            complement(1052..2608)
                     /locus_tag="PLB33_08435"
     CDS             complement(1052..2608)
                     /locus_tag="PLB33_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOG63592.1"
                     /translation="MESKLNLDPRLIDSARIAARNIADDVQKFIDKNTTVTVERTIAR
                     LMGVDGVDEIEKPLPNVLIDSIKEGGGLSRGAGFWIGNAMVNTGKTPQEIAEKVSSGE
                     IEITRLPIADENKIMEAVYETAKQMVKKIKNSRAKRDEYLKKLGEGRKPYLYVIVATG
                     NIYEDVLQAQAAAKQGADVIAVIRTTAQSLLDYVPYGPTTEGFGGTYATQENFRIMRK
                     ALDEVGEEVGRYIRLCNYCSGLCMPEIAAMGAIERLDVMLNDALYGILFRDINMQRTL
                     VDQYFSRIINGFAGIIINTGEDNYLTTADAYEEAHTVLASQFINEQFALKAGLPEEQM
                     GLGHAFEMNPMLENSFLYELAQAQMAREIFPKAPLKYMPPTKYMTGNIFRGHLQDAMF
                     NLISIWTHQGIQLLGMPTEAIHTPHLQDRMLSIENAQYIFNNARDIGNEIEYKKDGII
                     QKRAQEVLAKAEELLKDIEKEGIFNTIEKGKFGGVKRSFTGGKGLEGVVEKDEKYFNP
                     FIDLMLGGAK"
     gene            complement(2685..4283)
                     /locus_tag="PLB33_08440"
     CDS             complement(2685..4283)
                     /locus_tag="PLB33_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOG63593.1"
                     /translation="MNSFMTENTIKNVNFDYIFNEVKPITEYGIRTKQEAKPFIKGQE
                     ADLKAEFNKIGAFIESRQRRDIIDVLKHIKNILETIQRAKSNQVLDEVELFEIKNFLI
                     LVEKMDKILRGISNNDLKLLQLTPLPQLYEKLDPAHEKLNTFYIYDEYSQKLKKIRNE
                     KRQIEKNISLLKKRIREEIQEKHNIKLNLKDEVTISKSQKDKINELNKEVNLRLFGEN
                     YLNIIYKIKNSEEIDDLEKRYEELTFEEDDEEYKIRQKLSVSIKEFSHILQKNTEIIG
                     EIDYLIAKANYAQKTCSVEPEIINELQITIKGGRNLKLEKILKDKHIEYVPIDLNLNK
                     NVSCITGANMGGKTVTLRMIGQIAAMASFGMFVPCEYARLCLFERIFISAGDNQSIEK
                     GLSTFGAEIVNLKEAIKSSDLRCLILIDELAGGTNPKEGYAITKAVVNYLKKKDCITV
                     LTTHYDNIANDEDIKNLQVKGLSLPDETDFSKQDNIDSIQKYMDYRLIEVNYSGDIPK
                     DALRIAKMAGIDEEIIKDAENYLK"
     gene            complement(4280..5308)
                     /locus_tag="PLB33_08445"
     CDS             complement(4280..5308)
                     /locus_tag="PLB33_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63594.1"
                     /translation="MRLIETIKQNNYETVSIIGLAKNAGKTVTLNYLIEEAINLNIKT
                     GITSTGRDGENTDLITKTQKPAISVTEGMYVATAKKTLMLSNAKAEILETTGISTAMG
                     EVIIIRVRQKGNIQIAGPVSAKDMKYVARRLKYYGAEVVFIDGAIDRKAVSSPAITDA
                     CVIATGAVLSRDMKKVLEKTAHAVECYSLTGTDEHVKNIVRKINKTCIISEDGKTLVP
                     DIKTSITSGKKISEMISEKTTHVFVKGAVTSALLKDLWENKYLRGIKLIIEDGTKIFT
                     DINMWNEMRRKGLKVETINTINVLAVTLNPVSPEGYFFDSEVLKENMKKVLTGIKIVD
                     VVSGGEED"
BASE COUNT         1400 a         1152 c          808 g         2077 t
ORIGIN      
        1 ctatattatt ggccaaaaaa agcacaaaga ttactataag cactatcagc ctcggctgat
       61 gtgcattttc aaaataaagt gccgataaaa ataagatgat ggttatatta aatttaagaa
      121 aatataaaat atttttggtt ttcatcataa aaactccgta aaacgtgaac tttatagctg
      181 ttactatttt atttatttta ccattttttt gtatttttgc aatggatgtt ttagaaaaca
      241 taaaaaaaag gtgccaggca ccttttcagg tgcctggcac ctcaatataa ctcttagatt
      301 aaatttcttt gaacaatttg ttttaccaca aatgatgcaa cgtggttagc atagcttcct
      361 gtcccaaaac ctgcatcata tcctaattcc tgtgcaagct catgatttat acggggccct
      421 ccgcaaacta aaatcacttt atccctgagt ccttctgctt ctaccatctc aactaattgt
      481 gtcaggttgg aaatatgaat atttttttgt gtaactactt gggataccaa tattgcatct
      541 gcttttactt caatggccct tgcaataagt tcttcgtttg gtacctggct gcccatgttg
      601 attgcttcaa tgcccttgta tctttcaagt ccgtaattac cgtcaaagcc tttcatattc
      661 attatggcat ctattccaac ggtgtgagca tcagtacctg tgcatgctcc tacaatcact
      721 acgtcgcgct tgatattttc ctttatatat tcttcaactt cttctctttg cataaattca
      781 atatctgcct ttggaacttg tatcgaagta taatcaacgg tatgagtgca ttttccatac
      841 attatgaaat atgtgtatcc tactcccaaa tcttttgaaa aaacaaccgc cggattttca
      901 aatcccattt tagataccaa ttgtttcgct gcttcatttg cttcgtcgcc gtaaggaacg
      961 ggtaatgtaa aactcatttg aaccattcca tcattcaatg tatctccata tggtttgatt
     1021 ttcgttaaat ctactttctg gataccttcc attattttgc accccccaac attaaatcaa
     1081 taaatgggtt aaagtatttt tcgtcttttt caacaacgcc ttccaatcct ttgccgccgg
     1141 taaagcttcg ttttacacca ccgaatttac ctttttcaat tgtattgaat attccttctt
     1201 tttcaatatc ctttaacagc tcttcagctt tagccagcac ctcctgtgct cttttctgaa
     1261 ttattccgtc ttttttgtat tcaatttcat tgcctatatc acgagcatta ttgaaaatat
     1321 actgtgcatt ttctattgaa agcattctgt cctgaagatg cggtgtatga attgcttccg
     1381 taggcatccc caacaattga attccctgat gagtccatat ggaaattaaa ttaaacattg
     1441 catcctgtaa atgtcctctg aatatatttc ctgtcatata ttttgttgga ggcatgtatt
     1501 taagcggtgc tttggggaaa atttctcttg ccatctgagc ctgagccaat tcgtacaaga
     1561 agctgttttc taacatcggg ttcatttcaa aagcgtggcc gagacccatt tgctcctctg
     1621 gaagtcctgc ctttaatgca aactgctcgt taataaactg cgatgccaat acggtgtgag
     1681 cttcttcata agcatctgcc gtagtcagat agttgtcttc tcccgtattt atgataatac
     1741 ctgcaaagcc gttgattatt cttgaaaaat attggtcaac caatgttctt tgcatgttta
     1801 tatctctgaa aagaattccg taaagagcat cgttaagcat tacatcaagc ctttctattg
     1861 ctcccattgc tgctatttca ggcatgcaaa gacctgagca gtagttgcaa agacgaatat
     1921 atcttcctac ttcttctcca acctcatcaa gagctttacg cattatcctg aagttttcct
     1981 gagttgcata agttccgccg aagccctcgg ttgtaggtcc gtaaggaaca tagtcaagca
     2041 agctttgagc tgttgttctt ataacggcaa ttacatcggc accttgcttt gcagccgcct
     2101 gcgcctgtag aacatcttca taaatattgc ctgttgcaac tattacatac aaatatggtt
     2161 ttcttccttc accgagtttt tttaagtatt catccctttt tgctctgctg tttttaatct
     2221 ttttaaccat ttgctttgca gtttcataaa ctgcttccat aattttgttt tcatcggcaa
     2281 ttggcagtct tgttatttca atttctcccg agcttacttt ttctgctatt tcctgagggg
     2341 tttttccggt attaaccatg gcatttccta tccagaatcc tgctcctctg gataatccgc
     2401 cgccttcctt aatagaatct atcaatacat tgggaagggg tttttctatt tcatcaacgc
     2461 catctacccc catgagacgt gcaatagtcc gttcaactgt aacagttgta tttttatcaa
     2521 tgaatttttg tacatcgtct gcaatatttc ttgctgcaat acgtgcactg tcaattaatc
     2581 ggggatcgag atttaattta ctttccatac atcctcctct tacggggaca ttccttcatg
     2641 gtccggcctc aacggcttac tctccatgaa gagtgtcgcc cttcctattt taaataattc
     2701 tctgcatctt ttattatttc ttcgtctatt cctgccattt ttgctatcct taaggcatct
     2761 ttcggaatat ctccggaata attcacttct ataagcctgt aatccatata tttttgaatg
     2821 ctatcgatgt tgtcttgttt gctaaaatca gtctcatcag gaagactaag cccttttacc
     2881 tgcaaatttt ttatgtcttc atcatttgca atattgtcat aatgagttgt aagtactgtt
     2941 atgcaatcct tttttttaag ataatttact actgcctttg taattgcata accttccttt
     3001 ggattagtac ctcccgccag ctcatcaatc aaaatgagac atcttaagtc agagcttttt
     3061 attgcttctt ttagatttac tatttcagct ccaaaggtag aaagaccttt ttcaattgac
     3121 tgattgtcac cggcagaaat aaatatgcgc tcaaaaagac ataatcttgc atattcacag
     3181 ggtacaaaca taccaaagct agccattgct gcaatttggc ctatcatcct aagggttacg
     3241 gttttccccc ccatatttgc ccctgttatg caagacacat ttttattaag atttaaatca
     3301 atgggaacat attcaatatg tttatctttc aatatttttt caagtttaag gtttctgcct
     3361 cccttaattg tgatttgcag ctcatttatt atttccggct ctacggagca ggttttttgt
     3421 gcataatttg cttttgcaat aagatagtcg atttcaccga taatttctgt gttcttttgc
     3481 aatatatggc taaattcttt tattgacacc gagagttttt gtcgaatttt gtattcttcg
     3541 tcatcttctt caaaagtgag ttcttcgtac cttttctcca ggtcatcaat ttcttcggaa
     3601 tttttaattt tataaataat atttaaataa ttttctccga ataatcttaa attgacttcc
     3661 ttattcagct cattaatctt atctttttgt gacttgctta ttgttacttc atcttttaaa
     3721 tttaatttaa tattatgctt ttcttgtatt tcttctctga ttcttttttt taataaactg
     3781 atatttttct ctatctgtcg tttttcattt cgaatttttt ttaatttttg cgaatattcg
     3841 tcgtaaatat aaaatgtgtt taacttttca tgagccgggt caagtttttc atatagttgt
     3901 ggcaggggcg tcaattgtaa tagctttaaa tcattattgg aaatgcctct taaaatttta
     3961 tccatctttt cgactaaaat caaaaagttt tttatttcaa acaattctac ttcatcaaga
     4021 acctgattgc tttttgccct ttggatagtt tcgagtatat ttttaatgtg cttcaaaaca
     4081 tcgattatgt cccgcctttg tctgctttca ataaatgccc cgattttatt gaactccgcc
     4141 ttaaggtctg cctcctgacc ttttataaaa ggcttagctt cctgttttgt ccttatcccg
     4201 tattctgtaa tcggttttac ttcattgaaa atataatcga aatttacatt ctttattgta
     4261 ttttccgtca taaagctatt caatcttctt caccgcccga caccacatca actattttta
     4321 ttcccgtcag gacttttttc atattttctt ttaaaacttc actgtcaaag aaatatcctt
     4381 ccggagatac cggatttaaa gttaccgcca gaacatttat tgtatttatg gtttcaactt
     4441 taagtccttt ccttctcatt tcattccaca tatttatgtc cgtaaatatt tttgttccgt
     4501 cctctataat taatttaatg cctcttaaat atttgttttc ccataaatct ttcaacaagg
     4561 cagatgtaac cgcaccttta acaaacacgt gagttgtttt ttcactaatc atttcactga
     4621 tttttttgcc gctcgtaata cttgttttaa tatcgggaac aagagtttta ccgtcttctg
     4681 atatgatgca tgttttattg attttccgca ctatattctt aacatgctca tccgtacctg
     4741 taagcgagta gcattccaca gcatgggcag tcttttctaa aacctttttc atatctctgc
     4801 taagtacagc acctgttgca ataacacatg catctgtaat agccggagat gaaacagctt
     4861 tcctgtctat tgctccatct atgaatacca cttctgctcc gtaatattta aggcgcctcg
     4921 caacatattt catatctttt gcgctgacag gtccggcaat ctgtatattg cctttttgtc
     4981 tcacccttat aattattact tcgcccatgg cagttgatat ccccgtagtt tccaatattt
     5041 cagccttagc gtttgaaagc atcaatgttt ttttcgcagt ggcaacatac attccttcag
     5101 ttaccgaaat tgcaggcttt tgtgtttttg ttattaaatc tgtattttca ccgtcgcgcc
     5161 ctgttgaagt aatacctgtt ttaatgttta aatttattgc ttcttctatg agataattaa
     5221 gtgtcacggt ctttcctgca ttttttgcaa gacctattat ggatacagtt tcataattat
     5281 tttgttttat ggtttcaatt aatctcatag aaaacctctt atagtcagtt cattgttatc
     5341 attatagcgc agtcccgctg cacaatttcg tctgctcctt tcgtcgcata aaattggcgc
     5401 cggttagggc atctcgcagt ctcattcacc aatgtat
//
LOCUS       DAOSHP010000106         5388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_13475_length_5388_cov_11.481530, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000106 DAOSHP010000000
VERSION     DAOSHP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13475_length_5388_cov_11.481530"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..527
                     /locus_tag="PLB33_08450"
     CDS             60..527
                     /locus_tag="PLB33_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63595.1"
                     /translation="MKRIIVLLILITITATGCSQEVSDPTGAYSLVIDKLYNEDSALN
                     HEIKYIAVDTSLIKNLSEEQKSALLKEVEKYGFEVLDMTIEELKEEGYIEDLFFKEGI
                     LFKLEDEPIGGNSILMNVSKWRSGLGAIGYDDLKVKYKNGKWQITDLGRAWIS"
     gene            complement(550..1119)
                     /locus_tag="PLB33_08455"
     CDS             complement(550..1119)
                     /locus_tag="PLB33_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOG63596.1"
                     /translation="MNEFKREYPLFSLCGLNCGLCPRYHTNGTSKCPGCGGEDFYLKH
                     PSCAVINCSRKHGNSEYCYQCKLFPCEKYTKPNTSDSFISYNNVNTDLEKAKCDIINY
                     KSELNEKVEILRFLLDNFNDGKRKNFYCLAVNLLELEDLREIAAEIKDKISSMASSDK
                     DKIQNIVLLLQNKAGEYGVDIKLRKQEAK"
     gene            complement(1255..2412)
                     /locus_tag="PLB33_08460"
     CDS             complement(1255..2412)
                     /locus_tag="PLB33_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG63597.1"
                     /translation="MSNVVFLGLISFFADISAEMVYPIIPLYLTSSFGATPAMVGLIE
                     GIAESLASLLKVFSGYITDKYQKKKPIAFVGYATGIIYKAALLFAQSWTGILGARVID
                     RIGKGIRTAPRDVMISDSSQDRHGGTAFGIHKALDMAGSALGILIAYLILKSSNGIYD
                     YKQIFVVSIIPAALSLLMFFKIKEKKEERFVQERRPFWKNVKEVDNQLKLYLFIAFLF
                     TLGNSSNAFLLLKAKSIGFDDTTVILLYFIYSITASILAVPAGNRSDIAGRKKLLVAG
                     YIIFALVYFGFAFVNNKRFMIVLFVLYGVYTAMTAGVERAYISEISPKELKGTMLGLH
                     ATVTGIALLPASAIAGLLWNSFGARAPFIFGSSLSLAAAFTLINFMNEVKH"
     gene            complement(2558..4324)
                     /locus_tag="PLB33_08465"
     CDS             complement(2558..4324)
                     /locus_tag="PLB33_08465"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOG63598.1"
                     /translation="MIEGVNLKKIFKVYNLVLLIILTCFLIGCGSPAVNTAKMGNTNA
                     NIVTGGTSVKSDDWIYFVNYADSNFLYKIKTDGTGETKISDDAAFCLNFYKDWLYYVN
                     SSDNNKIYKIKTDGSERTVVSDISSANLLVYDDWIYFINNTFEESSDDYNKVFRIKTD
                     GTGKEKINNDKTSMFNIVGDNLYYVEQNEQKMYRIKTDGSDKTKIIDAKISFFNVVGN
                     NIFYLDAEDENNNIMKMNIDGTGTVKLSEDKAAPFNVSGDWIYYGNTIKDSPDMELKR
                     MKLDGTGAAVINDDNAMVINVHDDWLVYIGINFNDFSIKQTIIKEDGSGRKDYLYTQT
                     PQAQNAERYPMKETVSIGDISVTVNSAYSTNIVKYDQPGYDSQIFDYVTDGVYLYINT
                     TIANEGIKEIDLMQMTGIIEDIEAMGLNVTWGPMADITDEPGKDNISFHLANDRYMES
                     IMIESGEAKDLQLYYELYYGLDKTLYPVYLGIFDGTNTNPLAIFEVFPDDEYYVTSYE
                     SAVEIMNERFPEYEITMLQGVGHKFEGEDIEKMYYLFETKKKGASETEYFLVKRDTGE
                     IFNGKYDQRFPDYPAVPVSLLE"
     gene            complement(4466..5257)
                     /locus_tag="PLB33_08470"
     CDS             complement(4466..5257)
                     /locus_tag="PLB33_08470"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG63599.1"
                     /translation="MIYNNINVTNKPERSSITIGSSAPDFIALSTKGYIRLSDYKGKW
                     LILSSHPSAFGAVSTTELISAAQNYQFYKDRNIEIMGLTTDNINANLAWVNDIYQRTG
                     IEIPFPIISDNDLAISEAYGMISPDRMFGETVRGIFIIDPTGHIKTIFTLPVSTGASR
                     QEFTRIIDSILLNDEYKVSTPANWRPGAPVIVPNPVSYEEMVSRAENNERLGYYCPFW
                     YLCYTNLSTAPGQGNNNTNVPPKVNNNMNVTPQGNNIFKFLDAVF"
BASE COUNT         1679 a         1023 c          803 g         1883 t
ORIGIN      
        1 attaatgttt agaacactta aaaagtagtt ctaaacatat tatacgagga ggaattaaca
       61 tgaaaagaat aatagtactt ttaatattaa taactataac tgcaacaggc tgcagtcagg
      121 aagtatctga tccgacgggt gcctattcct tagtgattga caagttatat aacgaagatt
      181 cggccttaaa tcatgaaatt aaatacatcg ctgttgatac gtcattgata aaaaatttat
      241 cagaggaaca gaaatccgcc ttgttaaaag aggttgaaaa gtatggattt gaagtgcttg
      301 atatgactat tgaagagctt aaagaagaag gttatattga ggatttgttt ttcaaggaag
      361 gaatcttgtt taaattagaa gatgaaccaa taggcggaaa ctcaatactc atgaatgttt
      421 caaaatggcg ttccggtctt ggggctattg gttatgatga tttgaaggtt aaatataaaa
      481 acgggaaatg gcaaattaca gaccttggaa gggcttggat tagttagtat tatcctgtag
      541 ataataatat cacttggctt cttgtttcct tagttttata tctactccat attcaccagc
      601 cttattctga agcaataata caatattctg aattttatcc ttatcagagc tcgccatact
      661 gcttatttta tcttttattt ctgcagcaat ttcccttaaa tcttctaatt caaggagatt
      721 aactgcaagg caatagaagt tttttctttt cccatcattg aaattatcca ataaaaaccg
      781 gagaatttca actttttcat ttaattcgct tttgtaatta attatatcac attttgcttt
      841 ttctaaatct gtattgacgt tattatatga aataaaggaa tctgaagtat ttggcttagt
      901 atatttctcg catggaaaca atttgcattg ataacaatat tcactgttgc catgtttcct
      961 gctgcagttg ataacagcac aggatggatg ttttaaataa aagtcttctc cgccgcaccc
     1021 cggacacttt gatgttccat tagtatgata acgtggacaa agtccgcaat ttaatccgca
     1081 taatgaaaat aatggatatt ccctcttgaa ttcattcact cttatcaccc ctgctccttt
     1141 tataatcaga tttatttaca aatattgaaa taatgaattt taaaaattca tattattttg
     1201 ttattcttgc tgccggataa ttccccgaaa acattgaaaa tattaagaac aactttaatg
     1261 ttttacttca ttcataaaat taattagtgt aaatgctgct gctaatgaca aactactgcc
     1321 aaatataaaa ggtgctctag caccaaaaga attccaaagg agtcctgcaa ttgcacttgc
     1381 aggaagcagt gctatccctg taactgtagc gtgaagtcca agcattgttc cttttaattc
     1441 tttcggagaa atttctgata tataagccct ttctactcct gcagtcatgg cagtataaac
     1501 accgtagaga acaaaaagca caatcataaa tcttttattg ttaacaaatg caaatccaaa
     1561 atacaccaaa gcaaaaataa tatatcctgc aacaagcagt tttttccttc ctgctatatc
     1621 agatctattt cctgcaggta cagcaagtat tgaggcagtt atactgtaaa taaaataaag
     1681 caaaataacg gtggtatcat caaacccgat gcttttagct tttaacagca gaaaagcatt
     1741 ggatgaattc cctaaagtaa acaaaaatgc tatgaataga tatagcttca attgattatc
     1801 tacttctttt acattcttcc agaatggtct gcgttcttgt acaaaccgct cttctttttt
     1861 ttctttgatc ttaaagaaca taagcaggct aagagccgca ggtattattg aaacaacaaa
     1921 tatttgctta taatcataaa ttccatttga acttttaaga attaagtagg caattaaaat
     1981 tcctaaagct gaaccggcca tatcaagagc tttatgtatt ccgaatgctg ttcctccatg
     2041 cctatcttga gaactatcac ttatcatcac atctcttgga gctgttctta ttccttttcc
     2101 aattctgtca ataacccttg ctcccagaat gcctgtccag gattgtgcaa ataacagtgc
     2161 tgctttatat ataatcccgg ttgcataacc aacaaatgct attggtttct ttttctgata
     2221 tttatcagta atgtatccgc taaatacctt caatagactt gccagacttt cagcaattcc
     2281 ttcaataagg ccaaccattg ccggtgttgc cccaaatgat gatgttaaat acaagggtat
     2341 aataggatat accatttctg cactaatatc agcaaaaaaa cttattaatc ctaaaaaaac
     2401 aacatttgac acttttgtca ctccttcaat aattgtccag gcattggcta tattatacca
     2461 aattaatttt aaaattacta ttaaaaaccg gcctcgttga aaataaaagg ccggttatta
     2521 atatcttttt catgttccgt ttcaaattca tttgaagcta ttccaacaga cttacaggta
     2581 ctgccggata gtcgggaaac ctctggtcat atttgccatt gaatatttct cctgtatcac
     2641 gctttaccag gaaatattca gtttcagatg cacccttctt tttagtttca aacaagtaat
     2701 acattttctc tatatcttcg ccttcaaatt tatgccccac cccttgaagc atagttatct
     2761 catattccgg gaacctttca ttcatgattt ctacagctga ttcataactt gtaacataat
     2821 attcatcatc agggaagact tcaaagatag caagaggatt tgtatttgta ccgtcaaata
     2881 ttcccagata aactggataa agagttttgt ccagcccata gtataattca tagtataatt
     2941 gcagatcttt tgcttcgccc gattctatca ttattgattc catgtaccta tcattggcaa
     3001 gatggaaaga tatattatct ttacccggtt catctgtaat atcagccatc ggaccccagg
     3061 ttacattcaa tcccatcgct tctatatctt caattatacc tgtcatctgc attaaatcga
     3121 tttcttttat accttcatta gctattgttg tattaatata taagtatacc ccatcagtaa
     3181 cataatcaaa tatctgggag tcatagccgg gttggtcgta ttttacaatg ttggtcgaat
     3241 aagctgaatt tacagttaca gaaatatccc ctatacttac agtttccttc ataggatatc
     3301 tttcagcatt ttgtgcttga ggagtctgag tgtataaata atccttcctg ccactaccgt
     3361 cttccttaat tattgtttgt ttgatgctga aatcattaaa atttattcca atataaacga
     3421 gccagtcatc gtggacattt attaccatag cattatcatc atttattacg gctgccccgg
     3481 ttccgtccag tttcattctt ttcagttcca tatcaggtga atcttttatt gtatttccgt
     3541 aataaatcca atcaccgctt acattgaaag gcgctgcctt gtcctctgac agttttacag
     3601 tacctgtacc atcaatattc attttcatta tattgttgtt ttcatcttca gcatctaaat
     3661 agaaaatgtt attcccaaca acattaaaaa atgaaatctt agcatcgata atctttgttt
     3721 tatcggaacc gtcagtcttt atcctataca ttttctgttc attttgttca acatagtaca
     3781 ggttatctcc cacaatatta aacatgcttg tcttgtcatt atttatcttc tcttttcctg
     3841 taccatcggt tttaatcctg aaaaccttat tgtaatcatc agaagattct tcaaatgtat
     3901 tattaataaa gtagatccag tcatcatata caagcaaatt tgcagatgaa atatcactta
     3961 ccacagttct ttcagatcca tctgtcttga ttttataaat tttattattg tcactgctat
     4021 tgacatagta taaccagtcc ttatagaagt ttaggcaaaa tgcggcatca tcacttatct
     4081 ttgtctctcc tgtaccgtcg gtttttatct tgtaaagaaa attggaatcc gcataattca
     4141 cgaaataaat ccagtcatca ctctttacag aagtaccgcc agttacgatg tttgcattgg
     4201 tatttcccat ttttgctgta ttaacagcag gtgaaccgca tccaataaga aaacaggtta
     4261 agataataag taaaacaaga ttgtatacct taaaaatttt ttttaaatta accccttcta
     4321 tcattttatg tccctcctat accgaatatg tggagggcag ttgttcaccg cctccacact
     4381 ttatctttac ccaaatttac atattaataa cactgttaat aaatatattt ttattctttg
     4441 aaaagattca caatccttta atgagttaaa aaacagcatc taagaattta aagatattat
     4501 tcccctgtgg agttacattc atattgttgt taaccttcgg aggtacattg gtattattat
     4561 ttccttgacc aggtgcagtg cttaggtttg tatagcataa ataccaaaag ggacaatagt
     4621 atcctaacct ttcattgttc tcagctctgg aaaccatttc ttcatatgaa accggattag
     4681 gtactataac cggggctccc ggtctccaat ttgcaggagt acttacctta tattcgtcat
     4741 ttaatagaat cgaatcaatt attcttgtga attcttgacg gcttgctccc gtagatacag
     4801 gcaatgtaaa aattgttttt atatgacccg tggggtctat aataaaaata cctcttacag
     4861 tttctccaaa cattctatca ggacttatca taccgtaagc ttctgaaata gctaagtcgt
     4921 tgtcagaaat tatgggaaaa ggaatttcaa tccctgttct ttgatatata tcattcaccc
     4981 aggctaaatt tgcattaata ttgtcggttg ttaaacccat aatttctata tttctatctt
     5041 tataaaactg ataattttgt gcagcactta ttagttctgt agtgctgact gccccgaatg
     5101 cagacgggtg gctggataat attaaccact tgcctttata gtctgataat ctgatataac
     5161 ctttagtgga cagtgcgata aaatcaggtg ctgagcttcc tatggtgata gaacttcttt
     5221 caggcttatt cgttacattt atattattat atatcatggc atctccttta cacataaata
     5281 tataatattt tatgatactg atattagaat gttaatattt tatataatcc ttatactcct
     5341 tccttttctt gcacctattt caagagactt acaggtactg ccggcgga
//
LOCUS       DAOSHP010000107         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_13891_length_5274_cov_7.423644, whole genome shotgun sequence.
ACCESSION   DAOSHP010000107 DAOSHP010000000
VERSION     DAOSHP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13891_length_5274_cov_7.423644"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLB33_08475"
     CDS             complement(<1..1216)
                     /locus_tag="PLB33_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63600.1"
                     /translation="MTMILSVEIDNNIKIIEASKKGKELSVLRCMSIDTACVKDGKII
                     DMEGAVKIINEELKRSEVKTRKAYFVINSTEIMIRTIKLPFLKKNSDILSMLQIELQQ
                     RISADLSKYKIIYEVSNVTDENKIQYAEYIVYCVPLILIGQYIELAAKLKLKLLKIDI
                     SLNCINALYKNNFKFNDVVLNNSEIISFFNVSKNSVSFAVANNGLCDFHISSEFENNY
                     IEKSAEPHSQHGYWEVATDDDLFAALIAKFMRCYYSVSGNRSIDKMYIYGSFNQKYKE
                     AIKTKLNLSSEVISTVSDLTVEIPDNFELSKYFNTFLMLLCNNKNLDLNTDRKLKFKN
                     KCRYAAILLIIAAASTALSGFFNSQAVMRNEVTAMSSYIDDGENNQIYSVIEGIKNET
                     DYLKFYLQQAQEL"
     gene            complement(1221..1637)
                     /locus_tag="PLB33_08480"
     CDS             complement(1221..1637)
                     /locus_tag="PLB33_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOG63601.1"
                     /translation="MLSAYPACIYKERDGEYSVIFPDLNHLATCGETLDEALYMAVDC
                     LAGYLFEAEKEGMKVNSPSNMNDIDLSAEYDDYAEAFVNIITVDVEEYAKKHFEKSIK
                     KTLTIPLWLNNMAVDKNVNFSQVLQSALKEKLNIRE"
     gene            complement(1679..1864)
                     /locus_tag="PLB33_08485"
     CDS             complement(1679..1864)
                     /locus_tag="PLB33_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOG63602.1"
                     /translation="MTKFRDLEKIILSDGWKLKSCVGSHFQYVHPVKTGKVTIPNHAG
                     DIPKGVVSSVLKQAGLK"
     gene            complement(1961..3160)
                     /locus_tag="PLB33_08490"
     CDS             complement(1961..3160)
                     /locus_tag="PLB33_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOG63603.1"
                     /translation="MISYKCNVIDISGNKQRITCAGNSRKDVIDFLKLQEYTVVKIND
                     KKTINLGNISKNVKSKDLAVFCKQTHAMLKAGVTIVSILKILKQQTENRKLRGYIGLM
                     HEELQKGHTFSETLRLRKGAFPETFISMAEAGELSGNFDVIMDRLSIHYEKENKIENK
                     IKSAMTYPIILSIVCIAVVIFLLTSIMPTFVEMYASSGVPLPKLTLVIINIGNLLKNF
                     WFLIVLWIAAVIFITSSILRIHKFRVKKDYLKLQIPLVKNMIIKVAASRFSRTLSTML
                     GSGASLLGALETVSGVTGNLYICSKILEVKEDVRKGLPLSLLLQNQGVFPPMVYYMIK
                     IGEDSGSLEEVLNKTADFYDEEIETAIHRLTTLLEPVMIVIMAIVIGFIVISMVLPMF
                     EMVNTVQ"
     gene            complement(3240..4925)
                     /locus_tag="PLB33_08495"
     CDS             complement(3240..4925)
                     /locus_tag="PLB33_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOG63604.1"
                     /translation="MNSVCSFSKIGEILLNQNEITYAQLNEALKQQHNRKLGEILIKN
                     NYITEIILLRALSEQLKIDVVNLDNYYIDSDAANLLSEITAKRTLSIPLKAWEGKILI
                     AMEDPLNIFYIEDIETETGRKAEIVLACKSQIIESIEKHMGKRSAEKAVEDFDKEKLP
                     VSEETADENDENIISIANSPVVRLINSLISQALKMGASDIHIEPLENRIRVRVRVDGE
                     LQEILTPSKQTQSAIVTRIKIIAGMNIAEKRLPQDGRIEMKIDDTVVDLRISVIPTVY
                     GEKIAIRILDRNNFLKTKEELGFTLSNMEKFNSLLDVPNGIILAAGPTGSGKTTTLYA
                     ALYELNKLNKNIITIEEPVEYKIEGINQLQVNIKAGLLFSTGLRSILRQDPNIIMVGE
                     IRDEETAEIAVRAAITGHMVISTIHTNDAVSTIMRLKDMGIKPFLVAASLRGIVSQRL
                     VKKICNNCREEYAANVAEKSLMGIKNNLMLYKGRGCPKCYYTGYSSRIAIHEVLIIDK
                     AMRSAIQNEGSNDELAAIAEGAGMTTLRDNCRELVLAGVTTIEEFSQVIYEMP"
BASE COUNT         1631 a         1074 c          681 g         1888 t
ORIGIN      
        1 taagctcttg agcttgctgt aaataaaatt tcaaataatc cgtttcattt tttatccctt
       61 caatcacact gtaaatttga ttattttccc catcatcaat atatgaactc attgcagtaa
      121 cttcatttct catgacagct tgggaattaa aaaatccgga taacgcggtg gatgctgctg
      181 caattatcaa taaaattgca gcataacggc atttattttt aaactttaat ttcctatctg
      241 tgtttagatc taaattcttg ttattacaaa gcaacattaa aaaggtgttg aagtatttac
      301 ttaactcaaa attatcagga atttctactg ttaaatcgga tacagtgctg attacttcac
      361 tgcttaaatt caacttagtt ttgattgctt ctttgtattt ctgattaaaa ctgccgtaaa
      421 tatacatctt atctatagac ctgttcccgc tgaccgaata ataacatcgc ataaacttag
      481 caattagtgc tgcaaataaa tcgtcatcag ttgcaacctc ccaatacccg tgctgagaat
      541 gtggctctgc agacttttca atatagttat tttcaaactc agagctaata tgaaaatcac
      601 aaagaccatt atttgcaact gcaaaggaaa cagagttttt acttacattg aaaaatgaaa
      661 taatttcaga attattcaaa acaacatcat taaacttaaa attattttta tacaatgcat
      721 ttatacaatt gagggaaata tcaattttca ataattttag ttttaatttt gcagctaact
      781 ctatatattg accgattaaa atcaaaggaa cacaataaac aatatattcc gcatattgta
      841 ttttattttc atcagtaaca ttagatactt catagattat tttatactta cttaaatctg
      901 cagaaattct ttgttgcagc tctatttgca gcatggacag aatatccgag tttttcttta
      961 aaaaaggcag cttaattgtt ctaatcataa tttcggttga attaattaca aaataagcct
     1021 tccttgtttt tacttcgctc ctttttaatt cttcattaat tattttgaca gccccctcca
     1081 tgtcaatgat tttcccatcc ttaacacagg cagtgtcaat ggacatacat cttaaaacag
     1141 acaattcttt accctttttt gacgcttcga taatttttat attattatct atttcaacag
     1201 ataaaatcat tgtcataata tcactccctg atatttaact tctcctttaa tgccgattgc
     1261 aatacttgag aaaaattaac attcttatct acagccatat tatttaacca caatggtatt
     1321 gtaagagttt ttttaattga tttttcaaag tgtttctttg catattcttc aacatccaca
     1381 gtaataatat taacaaatgc ttcagcataa tcatcgtatt ctgccgataa gtctatatca
     1441 ttcatatttg aaggagaatt tactttcatt ccttcctttt cagcttcaaa caaatatcct
     1501 gcaaggcaat cgactgccat atacaaagct tcatctaaag tttcaccgca agttgccaaa
     1561 tgatttaagt caggaaaaat aaccgaatat tctccatccc tttccttata aatacacgcc
     1621 ggatacgcag acaacataat aactcacctt tccttatttt aatagtatga tatactcatc
     1681 atttcaatcc tgcctgtttt aatacagaac tgacaactcc ctttggtata tcacccgcat
     1741 ggttcggaat agttactttc cccgttttta cgggatgtac atattgaaaa tgtgacccaa
     1801 cgcatgattt taatttccac ccgtcagata atattatctt ttctaaatct ctaaatttcg
     1861 taataaccac tccattatta ttatattatc atatacgtat tgtgtcaata cgtatccaga
     1921 ttttagggac aataaatata actcaactat tcaggcatgc tcattgcaca gtattaacca
     1981 tttcaaacat aggcaagacc attgatatca caatgaaccc tattacaatt gccataatca
     2041 caatcattac aggttccaac aatgttgtta atcgatgaat tgcagtttca atctcctcat
     2101 cataaaagtc agcggtcttg tttaatactt cttctaaaga gcctgaatcc tctccgattt
     2161 taatcatgta ataaaccatt ggcgggaaaa ccccttgatt ttgaagtaaa agagaaagtg
     2221 gtaatccttt ccttacatct tctttaacct ctaaaatctt gctgcaaata taaagatttc
     2281 ccgttactcc cgaaacagtt tccaatgcac caagaagtga agcaccactg cctaacatag
     2341 ttgacaaagt tcttgaaaac ctcgaagcag caacctttat aatcatattt ttaaccagtg
     2401 gaatttgaag tttgagataa tcctttttta ccctgaattt atgtattctc aaaatagaag
     2461 aagtaataaa aataacggca gcaatccata gaacaatcaa aaaccaaaaa ttctttaata
     2521 aattgccgat atttattatt accaatgtta atttaggaag cggaacaccg gaacttgcat
     2581 acatttcaac gaatgtaggc atgattgatg taagaaggaa tattaccacc gcaatacaaa
     2641 caatggaaag aataattgga taagtcattg cagattttat tttgttttca attttatttt
     2701 ctttttcata atgaattgaa agtctgtcca taattacatc aaaattaccg ctcaattctc
     2761 cggcttcagc catacttata aaagtctccg gaaatgcacc ttttctcaac ctcaatgttt
     2821 ctgaaaatgt atgtcctttt tgcagctcct catgcattaa ccctatgtac cctcttaatt
     2881 ttctattttc agtctgctgc tttaatattt tcaaaatgct gacaatggta actcctgcct
     2941 ttaacattgc atgtgtttgc ttgcaaaata cagccaaatc ctttgacttt acattcttcg
     3001 agatgtttcc caaatttata gtttttttat cgttaatttt tacaactgta tattcctgca
     3061 acttcaagaa atcaattaca tcctttcgag aattacctgc acaggtaatc ctttgcttat
     3121 tccctgatat atctataaca ttgcatttgt acgaaatcat aattactcct catgagctcc
     3181 ttcggcttcg cctcaggatg acaaattaca aatggggtta gggcatctga caaatggggt
     3241 tagggcatct cgtaaatcac ctgtgaaaat tcttctatgg tcgtaacacc tgccaatacc
     3301 aattccctgc aattatccct caatgttgtc atccctgccc cttcggcgat ggcagcaagt
     3361 tcatcattac ttccttcatt ttgaatagca gatctcatgg ccttatcgat tattaatact
     3421 tcgtgtatag caattctact gctgtatccg gtgtagtaac attttggaca tcccctgccc
     3481 ttgtacagca ttaaattatt tttaatcccc atcaaactct tctcagccac attagctgca
     3541 tactcttccc tacagttatt acatattttt tttaccaatc tttgagacac aattcccctt
     3601 aatgaagcag caaccaaaaa tggcttaatt cccatatcct ttagacgcat aattgtagac
     3661 acagcatcat tggtatgtat tgttgaaatg accatgtgcc ctgttatagc tgccctcacg
     3721 gcaatttccg ccgtttcttc atccctgatt tcgcctacca taataatatt aggatcctgt
     3781 cttaatatag accttaatcc tgtagaaaac aaaagtcccg ccttaatatt gacttgaagc
     3841 tgattaatcc cttcaatttt gtattccaca ggctcttcaa ttgtaataat atttttattc
     3901 agcttattta attcatacaa agccgcatat aacgttgttg ttttgccgct tcccgttggt
     3961 cctgccgcca aaattattcc atttggaaca tccaataatg aattaaactt ttccatattg
     4021 ctcaatgtga atcctaattc ctcttttgtt ttaagaaaat tattcctgtc caatattcga
     4081 attgcaattt tttcaccata cacagttgga atcactgaaa tacgaaggtc aacaaccgta
     4141 tcatcaattt tcatttcaat tcgcccgtcc tgaggcagcc tcttttccgc aatattcatt
     4201 ccggctatta ttttaatcct tgttactatc gctgactgtg tctgctttga aggagtaaga
     4261 atttcctgca gctcaccgtc tactctgacc cttaccctta ttctgttttc taatggctca
     4321 atatgaatgt cgcttgctcc catcttcagt gcttgactga ttaatgaatt tattaatctt
     4381 acaacaggtg aattagctat gcttataata ttttcatcat tctcatccgc cgtttcttct
     4441 gaaaccggta atttttcctt gtcaaaatcc tcaactgcct tttctgcact tcttttgccc
     4501 atgtgctttt caattgattc aataatctga gatttacaag caagcacaat ttctgccttt
     4561 cttcctgttt cagtctcaat atcctctatg taaaatatat tcagaggatc ctccattgca
     4621 ataagtattt ttccttccca tgcttttagc ggtatggata aagttctttt agctgtaatt
     4681 tctgacaata aatttgccgc atcagaatca atataataat tatctaaatt tacaacgtca
     4741 atttttaact gttcggataa tgcccttaac aagataattt ccgtaatata attattttta
     4801 atcaatattt ccccgagctt ccggttatgc tgctgtttca aagcttcatt caactgtgca
     4861 tacgttattt cattttgatt caataagatt tccccaatct ttgaaaaact gcatacgcta
     4921 ttcataaatt cactctcttc aagctatatt tgtttagtta taacttatta tgtatcttta
     4981 ggggtttcat tatattattc aaccacacct gtgtcaattt aactaaacgt tttattgatt
     5041 acagtaaaag ctacatttaa ttacaatcaa ttaatacaaa taaagtaaat gctgttatga
     5101 ttttccattt atgttatgaa ctttttataa ccttatacaa taaaaaatga cgattttcgc
     5161 gtcattgtaa tgaataagcg gacaaatgaa gatttaagcg gtacctatct atattgattg
     5221 ggtcaagtcc tttttttggt gtaaattcat tcggtaagaa tgtaatataa aata
//
LOCUS       DAOSHP010000108         5178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_14254_length_5178_cov_10.066367, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000108 DAOSHP010000000
VERSION     DAOSHP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14254_length_5178_cov_10.066367"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..443)
                     /locus_tag="PLB33_08500"
     CDS             complement(273..443)
                     /locus_tag="PLB33_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63605.1"
                     /translation="MRKFILMILLILVMLSFDGCDTRKDVSETPNTMSPSIMADGELY
                     STTEGEMPIEPY"
     gene            821..1963
                     /locus_tag="PLB33_08505"
     CDS             821..1963
                     /locus_tag="PLB33_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63606.1"
                     /translation="METLLEKLSQFEFLYSVKTNPYLPIVNFIVSRGFGADAASTEEV
                     MIAHGLGLSHEKILFSSPGKTRKDIEKTLDKSIIVADSYNELALINDVAKQRGLHIKA
                     GLRINPDYSMDFGKGASSKFGVDEETIVCQRDFLNNLTNISIVGIHVHLRSQVLEHSK
                     LYRYYEKIFELALFCKESMGWEMEFINFGGGLGIVYSSLNDIPLNLELLSVEFEKLLL
                     RFKDKISARLIIETGRFVVCEAGKYVTRIVDIKESRGTKYLVVENGLNGFLRPSIAEL
                     LTAYTPQDIKLQGCEPLFTSKDAFEFAILGKEKLPAEKVSIVGNLCTSADIMAKDIIL
                     PKADIGDILVVSKAGSYSYTLTPTLFSSHPLPLQFYLNENCEKCIL"
     gene            2021..2701
                     /gene="hypB"
                     /locus_tag="PLB33_08510"
     CDS             2021..2701
                     /gene="hypB"
                     /locus_tag="PLB33_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOG63607.1"
                     /translation="MTDIKLIKIKESITADNDRSADLIRNELANNKVFMINIMSSPGA
                     GKTSLIIATMKILQNKYHINVIEGDIDSTVDSEKIIRNGGDAVQIQTGGDCHVDASMI
                     KAAIETIDLKDYDVIFIENVGNLVCPAEFDTGANLKVMILSVPEGDDKILKYPLMFNV
                     CDALIINKIDCIEFFDFDFNVLKNRVSLINPKMKIFQVSAKTGEGIIEWCDWLSKQID
                     CYNKSLNL"
     gene            2729..3064
                     /locus_tag="PLB33_08515"
     CDS             2729..3064
                     /locus_tag="PLB33_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOG63608.1"
                     /translation="MTQKILSVVLQYAEFNDAEKVLEVCIEIGELRDFVESITQKYWD
                     YLSKGTIAEGAKIKFYRVPVSSICHSCSNIFSFDFRKPEKSSCPVCGSFDSELLTGTE
                     LEVKEILIL"
     gene            3140..4279
                     /locus_tag="PLB33_08520"
     CDS             3140..4279
                     /locus_tag="PLB33_08520"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG63609.1"
                     /translation="MENDLYKKLAKHLDNLPGGFPETDSGVEIRILKRLFTPEDAELA
                     LTLTLIEEEPHVIAKRAGISAEEATKRLSEMEKKGLIYGTHSKDNIDRYMALHFIIGI
                     WEFQVDKLNPDLVRDVDEYLPYWADVDTWKNVPQIRSIPVGEAIDSQLEITSYENAEE
                     LVDKQDKIAVAPCICRTEQQMIGKGCDKPLETCLSFGSGAEFYVRNGMGRFITKDEAF
                     NILKQANTAGLVLSPGNSQKATFICACCGCCCGVLRTLKTYPKPAEIVSSPFVARHNK
                     DTCIDCSVCVERCQMDALQSNNGKITLDKERCIGCGLCVTTCPTDSLILVRKPEDWQH
                     AVPKNFTLMNIKLGQHRGKLSMGRLMKMLVKSKLDRINSPKKWNL"
     gene            complement(4403..4891)
                     /locus_tag="PLB33_08525"
     CDS             complement(4403..4891)
                     /locus_tag="PLB33_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63610.1"
                     /translation="MNPMISKFVDSKFMTEEQAQYVENAINNNESIVVSGHRSTGTRP
                     FMATLMGVAKKQYSTVQVKGFDDINADTEYLLILALDNLDFEALVGDAIGAKAPFISI
                     KEPEHPVSLMKVMKQQFKLGKGIGKKIHTIEAKKVDGVPYVDKIIEFCMDESGKIVKT
                     DL"
BASE COUNT         1740 a          779 c          973 g         1686 t
ORIGIN      
        1 agaaatagaa aaaatcaaaa tacaaaatat tttttagatt atagagttaa aaaatgatat
       61 ttccataacc ttctacactg gctatgattt gagttttata taactgtact ccaattccgg
      121 tcataaatgt ggtaaacgaa ccggccagct taatattctt ctacgccatt aattttcgca
      181 tactttgcat tcggaactgg aaaattgatt tccccctctt ttgctggtaa ttcagtttcg
      241 ttaattattg aagtaacagt attaattgca ctctaatatg gctctattgg catctctccc
      301 tcggttgtag agtacaactc tccatctgcc attattgatg gagacattgt gtttggtgtt
      361 tcagaaacat cctttcgagt atcacatcca tcaaaggata gcatcactaa aattaacagt
      421 atcattaaaa taaattttct catcttactg ctccttatac tacagtttca tatatacgac
      481 gcatagtaat actttattcg gctcaatata agaaaaatgc gaattaatat aaccatatgc
      541 ccaaatgact tgatttttga tttttgcaaa ggtctgcttt ttcaatgtga tttaccttta
      601 aaatttgcat ttaatatgta aataagttac aattatagca tgtttcaatt atatattatt
      661 actattaaat atgacacact tttatctatg tctcatattt ttttaaaatt tgcaataaag
      721 aggatttatt tatggaaaat aatttagaca ttattaaaaa attgatggaa gaatatgaag
      781 acctttctac atttataatg aaggtgttat ttctaagcaa attgaaacac ttttagaaaa
      841 attatcacag ttcgagtttt tatattcggt aaaaactaat ccctatttac ctattgttaa
      901 ttttatagta tccaggggat ttggagcaga tgcagcttca actgaggaag taatgatagc
      961 acatggatta ggcttatctc atgaaaaaat attattttca tctccgggaa aaacacgtaa
     1021 ggatattgaa aaaactttag ataaatcgat aatagtagca gacagctata atgagttagc
     1081 tttgattaat gatgttgcaa aacagagagg attacatata aaggcaggat taaggataaa
     1141 tcctgattac tctatggatt ttggaaaagg tgcaagcagc aaatttggcg ttgatgagga
     1201 aacaatagtt tgtcagagag actttttaaa taatttgact aatataagta tcgttggtat
     1261 tcatgttcat ctgcgctctc aggttcttga acacagtaag ctttatcgat actatgagaa
     1321 aatctttgaa ttagctttat tttgtaaaga gagcatgggt tgggagatgg aatttattaa
     1381 tttcggagga gggcttggta ttgtatattc atcattaaat gatattccac ttaatcttga
     1441 attgcttagt gttgaatttg aaaaattgct cctaagattt aaggataaga ttagtgcaag
     1501 gctaatcatt gaaacaggca ggtttgtagt ctgtgaagcc ggtaaatatg ttactcgtat
     1561 tgtagatata aaggagtcca gaggaaccaa atacttagta gtagaaaacg gacttaatgg
     1621 ttttctaagg ccttctattg cagagctgtt aacagcatat actcctcaag atataaagtt
     1681 gcagggttgt gaaccattat ttacatctaa agatgccttt gaatttgcta ttcttggtaa
     1741 ggaaaagctc cctgctgaaa aagtaagcat agttggtaac ttatgtacgt cagctgatat
     1801 aatggcgaaa gatataatac ttccaaaggc tgatattgga gatatattag tagtgtcaaa
     1861 ggctggaagt tattcatata cattgacacc aacattattt tcaagtcatc ctcttccatt
     1921 acagttttat ttgaatgaaa actgtgaaaa gtgtatactc taagcagtaa atctattttg
     1981 ggtataaata aataagaaat aaaattattg ggaggcagca atgacagata taaagttgat
     2041 taaaattaaa gaaagcataa ctgcagataa tgaccgttct gcagatctaa tacgcaacga
     2101 actggcaaat aacaaggtat ttatgattaa tattatgtct tcgcccggtg caggtaaaac
     2161 aagtttaatt attgccacta tgaaaatact gcagaacaaa tatcatatca atgtaattga
     2221 aggtgacata gattcaacgg ttgactccga aaaaattatt cgaaatggag gcgatgcagt
     2281 acagatacag acaggtggtg actgtcatgt tgatgcttcg atgataaaag ctgctataga
     2341 aactattgat ttgaaagatt atgacgtaat ttttatcgag aatgtcggaa accttgtgtg
     2401 tccggcagaa tttgacacgg gtgctaacct gaaagtaatg attctcagcg tacctgaagg
     2461 ggatgataag attcttaaat atcctcttat gtttaatgta tgtgatgcat taattatcaa
     2521 taaaattgat tgcatagaat tttttgactt tgactttaat gttttaaaaa accgtgtttc
     2581 tttgattaat cctaaaatga aaatatttca ggtttctgcc aagacagggg aaggaataat
     2641 tgagtggtgt gactggttat caaaacaaat agactgttac aataaaagtc ttaatttata
     2701 ggaggtgtgt cctattcatg aattgccttt gacacaaaag attctatctg tcgtattaca
     2761 atatgcagaa tttaatgatg ctgaaaaagt tttagaggtt tgtattgaaa ttggagaatt
     2821 gagagacttt gttgaatcaa taacacaaaa gtattgggat tatctgagca aaggaacaat
     2881 agccgaagga gcaaaaatta agttttatcg agttccggtt tcatcaattt gtcacagctg
     2941 cagcaatatt tttagttttg attttcgaaa gccggagaaa tcaagttgtc ctgtatgcgg
     3001 aagctttgac tcggaacttt taacaggaac agaacttgaa gtaaaggaaa ttttaatttt
     3061 gtaatgaatc tgcaaataaa taattcggtt gttatgagaa aaatacaatt tagaacaatt
     3121 ttttcagtgg gtgaaaataa tggaaaatga tttatataaa aaattagcta agcatttaga
     3181 caatttaccg ggaggatttc cggaaactga tagcggggta gaaatacgaa tattaaaacg
     3241 cctttttaca cctgaagatg ctgaactagc tttaacactg acattaattg aagaggaacc
     3301 tcacgtgatt gcaaagcgtg ctggaatatc tgctgaggaa gcaactaaac gattatctga
     3361 aatggaaaaa aaggggctca tctacggcac gcattcaaaa gataacatcg acagatatat
     3421 ggctttacat tttattattg gcatatggga atttcaagtg gataaattaa atcctgattt
     3481 ggtacgtgat gttgatgaat acttgcctta ttgggcagat gttgatactt ggaaaaatgt
     3541 gcctcaaatc agatcaatac cggtgggaga agcaatagac tcacagttgg aaatcacatc
     3601 ctacgagaat gcggaagagt tagttgataa acaagataaa attgcagtgg caccgtgtat
     3661 atgcagaact gaacagcaaa tgatcggaaa aggctgtgac aagcctttag aaacatgtct
     3721 gtcattcgga tcaggagctg aattttatgt gcgaaatgga atgggacgtt ttattactaa
     3781 ggatgaagcg tttaatatcc taaagcaggc aaatactgcc ggacttgttt tatccccggg
     3841 aaactcacag aaagctacct ttatttgcgc ctgctgcggt tgttgttgcg gggtgctaag
     3901 aacattaaaa acttatccta aaccagctga aatcgtatct tcaccatttg tagcccgtca
     3961 taataaggat acctgcatag attgtagtgt ttgtgttgaa cgatgtcaaa tggatgcatt
     4021 gcagtcaaat aacggaaaaa taaccctgga taaagagcgc tgcataggat gtggtttatg
     4081 tgttacaacc tgcccaacag attcattaat tcttgttaga aaaccagaag attggcagca
     4141 tgctgttccg aagaatttta ccctgatgaa cattaagctg gggcagcatc gtggaaagct
     4201 ttcaatgggc agattgatga aaatgttggt aaagtctaaa ttagacagaa taaattcacc
     4261 aaagaaatgg aacttataaa actcctttat tcaatgttaa gattttctaa tcccaacaat
     4321 ttataaatgg ccggaataat aaaggggcat agttgtcagt tgacaccatg tccctttgtt
     4381 taatgtaaat attcttttta cattaaaggt ctgtcttaac tatttttccg ctttcatcca
     4441 tgcagaattc tataatttta tcaacgtatg gaacgccatc gactttctta gcttctatag
     4501 tatgaatctt tttgccaatg cctttaccca gcttgaattg ttgtttcata accttcatta
     4561 atgatactgg atgctcaggc tcttttattg agatgaatgg cgctttagca ccaattgcat
     4621 ccccaacaag tgcttcgaag tctaagttat caagtgctag aattaatagg tattctgtat
     4681 ctgcatttat atcatcaaag ccttttacct gaacagttga atattgtttt ttagctactc
     4741 ccattagtgt agccatgaag ggacgagtac ctgttgatct atggcccgat actacgattg
     4801 attcattatt gttgatagca ttctcaacat attgtgcctg ttcttctgtc atgaattttg
     4861 agtctacgaa cttactgatc attggattca tttaaatcac tcctataaaa attatatatg
     4921 tgtattgtaa cacttttcct aatgattata aagtcttttt tcaaaaagac tgtgacatta
     4981 tatagaataa cataatggct aattgatagg atagaattta gatacaaaag taaatctgtc
     5041 ctgaatctgt cctaaggtgc ttttaattta atgctgatta ttattccata tttaaccaga
     5101 tgtgtaagaa atggaatgaa aactaaaaag acaccaaccc gttccggtca gtgtctctcg
     5161 tcaagtggtt aattacat
//
LOCUS       DAOSHP010000109         5107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_14530_length_5107_cov_9.289588, whole genome shotgun sequence.
ACCESSION   DAOSHP010000109 DAOSHP010000000
VERSION     DAOSHP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14530_length_5107_cov_9.289588"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /gene="uvsE"
                     /locus_tag="PLB33_08530"
     CDS             complement(<1..685)
                     /gene="uvsE"
                     /locus_tag="PLB33_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HOG63611.1"
                     /translation="MKIGYACLTVGVPGTKLRSCTMKNASPEVLEALIQSNLDSLNNV
                     LDYNINNGIKLFRISSDIIPFGSHPVNSLNWQEKFSSQLTEIGQKALFSGTRLSMHPG
                     QYTVLNSPDEKVVERAVDDLRYHAEFLDSLGLGEEHKIILHIGGIYGDKKAAMERFIH
                     QYNLLEANIRNRLVIENDDKQYNISDVLKIGFSQGIPVVFDNLHHQVNPDGKFTESEW
                     IKRCSATWKK"
     gene            complement(824..1357)
                     /locus_tag="PLB33_08535"
     CDS             complement(824..1357)
                     /locus_tag="PLB33_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63612.1"
                     /translation="MEGLVILIIWALINSFIRSANKKDNEGKTQKRTTQTKRKNIFST
                     FIEEIEKEMQSEKEKHRLEGPAQRTQPSQSARTTQWAKPPQPALPKKQEQPSMLSDLP
                     KSKVQTKPAPPPKPVGLEIDAIGGDEEIGGIKEARISKGSKNNLDLNLNRDILKGIIY
                     SEILSKPKSLRNMKIRC"
     gene            complement(1362..2351)
                     /gene="floA"
                     /locus_tag="PLB33_08540"
     CDS             complement(1362..2351)
                     /gene="floA"
                     /locus_tag="PLB33_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720829.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOG63613.1"
                     /translation="MPELLLTGGLAIFIIILLILFFTFVPVGLWITAFFSGVRVKIST
                     LIGMRLRRVVPSRIVNPMIKATKAGLDLNIDELEAHYLAGGNVNTLVDALIAAQRANI
                     PLVFERAAAIDLAGRNVLEAVQVSVNPKVIETPNISAVAKNGIEVIVKARVTVRANIE
                     RLVGGAGEETIIARVGEGIVTTVGSSAAHADVLENPDSISRTVLNKGLDSGTAFEILS
                     IDIADVDVGRNIGAKLQMDQADADKKIAQAKAEERRAMAVAKEQEMIAEVQAMKAKVV
                     EAESQVPLAIAAALKEGNMGVMDYYNMKNVLADTDMRDSISKATKEDNNKSGM"
     gene            complement(2353..3642)
                     /locus_tag="PLB33_08545"
     CDS             complement(2353..3642)
                     /locus_tag="PLB33_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOG63614.1"
                     /translation="MKLRKFGIIFIILLLLSSIALGAEVNTSDKVYVIPINGEINRAT
                     NYFVQNTIEELEREGAKAIIFEIDTYGGLIDQAQQIKDSIIGTDIPTISFVNNNAQSA
                     GVLITIASEKIVMAKNSTLGSAETIPNTEKVLSMWRAVLRDTARYRGRNSDIIEAMAD
                     KDIEIEGINEKGKLINLTSNEAVEYGIADLTANDYESILEYFNIQASEIVKVKEDFQV
                     KIAKAVTSSTLSTLLLAIGFIGIAVEIFTPGFGVGGIASIIAFGLYFGGNILAGNSNW
                     ISLTLFVIGLILIVVEGMVPGFGLPGISGIIFIVVGIIMAMNNLRSAVLSLSIAIILT
                     AIVVIILVKLGYRSKIFNNIILSNKLHKDKGYIPSEDMEHLLGEEGRALSELRPAGFI
                     EIGNNKYDALSDGGFITRNDKIKVVKVEGSKIFVRRI"
     gene            complement(3910..5025)
                     /locus_tag="PLB33_08550"
     CDS             complement(3910..5025)
                     /locus_tag="PLB33_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOG63615.1"
                     /translation="MKIVEGRFNSAVVFTDDLEDSSAAQIKTLCDQEYTKGSKIRIMP
                     DVHAGAGCTIGTTMTITDKLVPNLVGVDIGCGMETVKIKNSHIELSRLDKLIYEKIPS
                     GFSIRNAAHKYNEEIDLTRLRCIEGGKINLRRAILSLGTLGGGNHFIEAGRDEEGRTY
                     IVVHSGSRHLGFQVANFYQNQAYKYINKDIKTQIPKSLAYVSDKLMEDYIHDMKLVQK
                     FAMLNRKAIMDEIIKGMKLKAEDEFTTIHNYIDTENMILRKGAVSAQKGERLLIPINM
                     RDGSLICTGKGNSDWNCSAPHGAGRLMSRNQAKSSFTLSEFKKQMDGIYTSTVNNETL
                     DECPMAYKGIEDIIRNISDTAEVIKIIKPIYNFKAVE"
BASE COUNT         1387 a         1065 c          737 g         1918 t
ORIGIN      
        1 actttttcca ggtggctgaa cacctcttta tccattctga ttctgtaaat ttaccgtcag
       61 gattaacctg atggtggagg ttgtcaaaaa ctacgggtat cccctggcta aatccgattt
      121 tgagcacatc ggatatatta tactgcttgt catcattttc tataactaaa cgattcctga
      181 tattagcttc caaaagatta tattgatgta taaaacgctc catagcagct tttttgtctc
      241 cgtaaatgcc gcctatgtga aggataatct tatgttcttc tcctaagccc agtgaatcca
      301 gaaattctgc atgataccgc aaatcatcta ctgccctttc cacaaccttt tcatcgggag
      361 aatttaaaac agtgtattga ccagggtgca tagaaagacg cgtgccgctg aatagggctt
      421 tttgtccgat ttctgtaagc tggctgctga atttttcctg ccagtttaag ctattaaccg
      481 gatgcgaccc aaaaggtatt atatctgagc ttatgcgaaa cagctttatg ccgttattta
      541 tattatagtc taacacatta tttaaagaat ccaggtttga ctgaatcaaa gcttctaaaa
      601 cttcaggaga agcatttttc attgtacagg atctgagctt cgttcccgga acaccgacag
      661 taaggcatgc ataaccaatt ttcataaatc acctaaatct tctgaatttt acaagatctc
      721 ccttgttttt tattaaaact tatatatgac agctgccaca tttttaataa aacagccctt
      781 agttagattt taggtctaaa taagaggccg gattgttgtt gttttaacat cttatcttca
      841 tatttcttaa actctttggt ttggacaaaa tctcagaata tatgatgcct tttaatatat
      901 ctctatttaa gtttaaatct aagttattct ttgatccttt agaaattctg gcttccttaa
      961 ttcccccaat ttcctcgtct ccaccaatag catcaatttc taaacctaca ggtttaggtg
     1021 gcggtgctgg ttttgtttgg acttttgatt tagggagatc tgacaacatg gatggctgtt
     1081 cctgtttttt cggaagtgcc ggttgtggcg gttttgccca ttgtgtcgtt cttgccgatt
     1141 gcgacggttg tgtccgttgt gccggtcctt ctaatctatg cttttctttt tcactttgca
     1201 tttctttttc tatttcttct ataaatgtgc tgaaaatatt ttttcttttt gtttgagtag
     1261 ttcttttttg ggtctttcct tcattgtctt ttttattagc gcttctaata aaagagttta
     1321 tcaaagccca gataataaga atgaccagtc cttccattta atcacatccc acttttattg
     1381 ttatcctctt tagttgcttt agaaatagaa tccctcatat ccgtatctgc taatacattt
     1441 ttcatattat aataatccat tacacccata ttgccttctt ttaaagctgc agcgattgcc
     1501 aatggcacct gtgattctgc ttctactact tttgctttca tggcttggac ttcagcaatc
     1561 atttcttgtt ccttagctac tgccatcgct cttctttctt cagcttttgc ttgtgcaatc
     1621 ttcttgtcag catctgcttg atccatttgt agtttcgcac ctatgtttct tcctacatct
     1681 acatctgcaa tatcaattga taatatttca aaggcagttc cggaatctaa gcctttatta
     1741 agtactgtcc ttgatatgct gtcaggattc tctaatacat ctgcatgagc tgcagaagaa
     1801 ccaacagtgg taacaatacc ttcgccgact ctggctataa ttgtttcttc tcctgctcca
     1861 cctaccagtc tttcaatatt agcccttact gttaccctgg cttttacaat tacttcaata
     1921 ccattcttag caactgctga tatattagga gtttctataa ctttagggtt tacacttact
     1981 tgaactgctt ccaatacatt tctgcctgct aaatcgatag ctgccgctct ttcaaatacc
     2041 aagggtatat tagctctttg tgctgctatt aaagcatcta caagtgtatt tacattaccg
     2101 cctgccaaat aatgagcttc taattcatca atatttaaat caagacctgc ttttgttgct
     2161 ttaatcatag ggtttactat tctgcttggt actacccttc taagcctcat tccaataaga
     2221 gtagatatct taactctaac tcctgagaag aatgccgtaa tccataaacc aaccggtaca
     2281 aatgtaaaaa ataaaattag cagtataatt atgaaaattg ctaatcctcc tgttaataat
     2341 agttctggca tattaaatcc tccttacaaa tatttttgat ccttcaactt ttactacctt
     2401 tatcttatca tttcttgtta taaaaccacc atctgacaat gcgtcatatt tgttattccc
     2461 tatttcaata aaaccggcag gtctaagttc agataaagcc ctgccttcct cacctaatag
     2521 gtgttccata tcttcagatg gaatatatcc cttatcctta tgcagtttat tgcttaagat
     2581 tatattattg aaaattttac tccgataacc taattttact aatattatta ccacaattgc
     2641 cgtcaatatt attgctatac ttagtgataa taccgctgaa cgcaaattat tcattgccat
     2701 tattatacct acgacaataa atattatccc ggaaattccg ggcaggccaa atccgggtac
     2761 catgccttca actactatca ggattaagcc tattacaaat aacgtcagag atatccaatt
     2821 tgaattgcct gctaagatat tgcctccaaa atataatcca aaggcaatta tacttgctat
     2881 gcctcctact ccaaaaccgg gagtaaatat ctccactgct ataccaataa atcctattgc
     2941 caataataaa gtgcttaagg tagaacttgt aacagctttt gcaatcttaa cttgaaaatc
     3001 ttcttttact ttaacgattt cacttgcttg aatattaaaa tattccagta tactttcgta
     3061 atcattagct gttaaatcag ctataccata ttctactgct tcattgcttg tcaaattaat
     3121 caatttgcct ttttcattaa taccttctat ttcaatatcc ttatctgcca tggcttcaat
     3181 tatgtcagaa ttcctgcctc tataccgggc ggtatctctt aatacagctc tccacataga
     3241 taatactttt tcagtatttg gaatagtttc tgcagaacct aaagtagaat tttttgccat
     3301 aactattttt tcgcttgcta tagtgataag cacacctgct gattgagcat tgttattaac
     3361 gaatgaaata gttggtatat ctgtacctat tatagaatct tttatttgtt gagcttgatc
     3421 aattagtccg ccataggtat ctatttcaaa aattatagct tttgcccctt cccgctctaa
     3481 ttcttctatt gtattttgaa caaaataatt agttgctcta tttatttcgc cattaattgg
     3541 aattacatat actttatcac ttgtattaac ttctgcacca agagctattg aacttagcaa
     3601 caataaaatt ataaaaataa ttccaaattt tcttaatttc atatgagtat cacctgcctt
     3661 tcatttaata atgctggtct tttatcttat acccatattt cttagtaaat aacaatattt
     3721 tttctttgta ggtcataaat ttaatataac ataatatttt gtctcatagt gtaaattttt
     3781 tatcaaaaat agtctgctgt ttttaaccac cggactagtg ctctattctc tctattcttt
     3841 tggctcttct cttttgatat ttaaatgctc cttttttata attagttaaa ttttgattta
     3901 ttgcttttac tattcaactg ctttaaaatt ataaattggt tttattattt tgatgacttc
     3961 agcagtatca ctgatgttcc tgataatatc ttctattccc ttgtaagcca tggggcattc
     4021 atccaaagtt tcattattca cagttgatgt ataaattccg tccatttgtt ttttaaactc
     4081 tgataatgta aatgaagatt tggcttgatt cctggacatt aatctgcctg ccccgtgggg
     4141 cgccgaacag ttccagtctg aatttccttt gccggtacaa attaaggacc cgtcccgcat
     4201 gtttatcggt atcagaagcc tttctccttt ttgtgcagat actgcccctt ttcttaaaat
     4261 catattttca gtgtcaatat aattatgtat ggtagtaaat tcatcctctg ctttcagctt
     4321 catgcccttt attatttcat ccataatagc tttcctgttt agcatggcga acttttgaac
     4381 cagtttcata tcatgtatat agtcttccat gagcttatct gatacatatg ctaatgattt
     4441 tggaatctgt gttttaatat ctttatttat gtatttatag gcctggtttt gataaaagtt
     4501 tgcaacctga aatcccaggt gccttgaacc tgaatgcacc actatataag ttctgccctc
     4561 ttcatccctg ccggcttcaa taaaatgatt gccccctcca agggtcccaa gagaaagaat
     4621 tgctcttctt aaattgattt tgcctccttc tatgcatcta agccgcgtca agtctatttc
     4681 ttcattatat ttgtgggctg catttctaat gctgaaacct gaagggattt tttcataaat
     4741 gagtttgtca agtctgctaa gctctatatg cgaatttttt atcttaacag tttccattcc
     4801 acatccgatg tctactccta ccaaattagg taccagctta tctgttatag tcatggttgt
     4861 tcctatggta catcctgccc ctgcatgaac atcgggcata atgcggattt tagaaccttt
     4921 cgtgtattcc tggtcacata aggttttaat ctgcgctgca gacgaatcct ctaaatcatc
     4981 agtaaataca acggcactgt taaaccttcc ttctactatt ttcatcttat caaatctctc
     5041 tcctatatcc atgggtggtt ttatgtcagc ttttccataa taaaatcctt ttctataaaa
     5101 aattcct
//
LOCUS       DAOSHP010000110         4998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_14952_length_4998_cov_6.007890, whole genome shotgun sequence.
ACCESSION   DAOSHP010000110 DAOSHP010000000
VERSION     DAOSHP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14952_length_4998_cov_6.007890"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..4845)
                     /locus_tag="PLB33_08555"
     CDS             complement(55..4845)
                     /locus_tag="PLB33_08555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOG63616.1"
                     /translation="MKQISFIDAEVVPESGKISDFGAIRPNGEYIHGASPDVIRGFLL
                     ETDFLCGHNIIAHDLKYLNKYLGQIKCAGVIDTLHLSALLFPQRPYHSLVKDDKLLTE
                     EINNPVSDCKKAMNLFYDETGAFNLLPDEMKRILYILLNDKKEFKDFFKYIDYKNEGT
                     NLEAEAGIRKFFYGKLCENSDIKNIIYTHPIELAYCLSLICADDEYSITPPWVLMTYP
                     QVDNVMNQLRGTKCIPGCEYCNETMDAVKGLNRFFGYDAYRSFDGVPLQEQAVKSAIN
                     GDSLLAVFPTGGGKSITFQVPALMAGRNTKSLSVIISPLQSLMKDQVDNLENFNITDA
                     VTINGLLDPIERAEAIKRVETGDAHILYISPESLRSKSIEKLLQDRKIARFIIDEAHC
                     FSAWGQDFRVDYMYIAEFIKKICEKKNLDYIIPVSCFTATAKQNVIDDICKYFKENLN
                     LELKLYTADSGRKNLTYKIIKNESSEKYETLRNLIEYNNCPTIIYVSRIKNAMELAKR
                     LTDDGYEARPYHGKMDKKEKSKNQEEFIKGEVDIMVATSAFGMGVDKKDVGMVVHYDI
                     PGSLEDYVQEAGRAGRDQSINARCYVLFNDGDLDKHFILLNQTKISIQEIQQIWIAIK
                     NTTKFRSNMSNSALEIAKKAGWDEGMYDLETRVYTAVAALENAGYIKRGQNMPRVYAD
                     SIMARNMKEVSEKIAGSDIFSETEKQQAARIMNKLISKRSRSRAGSEDAESRVDYISD
                     HLGIEKAQVIHVIQLLRQAGILSDAKDLTIFMDESSYGKKSFDKLYEYIELERFLLSQ
                     ISEITTTKNIKQLNELALDAGVKNSNPDKIKTIFNFWSIKGLVKRNAKGNNNHIKINL
                     LRDRDKALEILEARWAIAEFILKHIQEINVNNENTLEFSVLELMGEYNFNMQLFNKKA
                     APWQIEDSLFYLSKIGILKIEGGFFVIYNAISIERLIDDNRIRYKAEDYKTLKDFYKH
                     KIEMIHIVGEYAKKMMEDYNSALKFADDYFRLEYSSFLRKYFKGPKGDEIQRNMTPER
                     FRQLFGQLSPAQLGIINDKVSKYILVAAGPGSGKTRILVHKLASVLQLEDVKNEQLLM
                     ITFSRAAATEFKKRLGDLIGTKANYIEIKTFHSYCFDLLGLVGNIEKSNDIINNAIDA
                     ILNNEVEQSRITKTVLVIDEAQDMDANEYRLVKTLMERNEDMRVIAVGDDDQNIFSFR
                     GSDSKYMSYILKFPNSKMYELVENYRSSRSIVDFSNYFVQNIKERLKSMPITAVTKEE
                     GNVAITKYKSSNLIVPVINKLLKDGILGSTCILTKTNEEALQIYALLDKNGIKAGMVQ
                     RGDNYDLKNLLEVRCFTEVLKLSNEAPLINDEEWEYAKKKTFNRFVKSENLPLLKQII
                     SDFEEIASKYKYKSDFSMFIEESQEEDFYNDTDKVTVSTIHKSKGKEFDHVIMVLKDL
                     NLNNEEIKRQLYVGMTRAKKTLSIHYEGEFIQRKKGFMGMGLRNIKYEFDNEEYPASE
                     IIAFQLKYSDVYLSNFYRTTRLVRRLNSGDMLRFDENGCLDKNGTQILLFSAKFKNEL
                     KTYVEKGYKPLSAKVNNILFWKQEDREKETLIVLPQLTFKKNPL"
BASE COUNT         1368 a          990 c          750 g         1890 t
ORIGIN      
        1 ccctacatgg aaatacccct tggaaaataa attttgtgac ataacctcat caagttataa
       61 tggatttttc ttaaatgtca gctgggggag gactatcaat gtttcttttt ctctgtcttc
      121 ctgcttccag aataatatat tgtttacttt ggcagacaat ggtttgtaac ctttttcaac
      181 atatgttttt aattcatttt taaactttgc tgaaaaaagt aaaatttggg tgccattttt
      241 gtctaaacat ccattttcgt caaatcgcag catatcaccg ctgttcaacc ttctaacaag
      301 tcttgttgtc ctgtaaaagt ttgatagata cacatcgctg tattttagtt gaaaagctat
      361 aatttctgaa gccgggtatt cttcattatc aaattcatat ttaatgtttc ttaagcccat
      421 acccatgaat cctttttttc tttggatgaa ttctccctcg taatgaatgg acaaagtttt
      481 tttagccctt gtcattccta cataaagctg tctttttatt tcttcattat ttaaatttaa
      541 gtcttttaat accattataa catggtcaaa ttccttccct ttggatttat gaattgttga
      601 gactgttact ttgtctgtat cattataaaa atcttcttcc tgagattctt caataaacat
      661 tgaaaaatcg gacttatatt tgtatttact tgcaatttct tcgaaatctg agattatttg
      721 cttcagaagc ggcaaattct cgctttttac aaacctattg aatgtttttt tcttcgcgta
      781 ttcccattct tcatcattta ttaatggagc ttcatttgat agttttaata cttccgtgaa
      841 gcatctgact tcaaggaggt ttttaaggtc ataattgtct cctctttgta ccattccggc
      901 ttttattcca tttttatcaa gcagtgcata tatttgaaga gcttcttcgt tggtttttgt
      961 tagaatacag gtagatccaa gtattccatc cttaagcagt ttatttataa cgggcacaat
     1021 gagattgctg cttttgtact ttgttatggc tacattgccc tcttcctttg ttactgctgt
     1081 aattggcata cttttcagtc gttctttaat gttttggaca aaataattgc tgaaatcaac
     1141 aatgcttctt gagcttctgt aattttccac taattcatac attttggaat ttgggaattt
     1201 caaaatatag ctcatatatt ttgagtctga acctctaaat gaaaagatat tctggtcatc
     1261 atcgcctaca gctattaccc tcatatcttc atttctttcc ataagagttt tcacaagtct
     1321 atattcattt gcatccatat cctgggcttc atctataacc aaaacagtct ttgtaattct
     1381 tgactgctct acttcattgt ttaaaatggc atctatcgca ttgtttatta tatcatttga
     1441 tttttctata ttcccaacta aacccagcaa gtcaaaacaa taggaatgaa atgtttttat
     1501 ttcaatgtaa tttgcctttg tccctattag gtccccaagt cttttcttaa actctgttgc
     1561 tgcagccctt gaaaatgtaa tcattaacag ctgctcattt tttacatctt caagttgcaa
     1621 aacagatgcc agtttgtgta caagaattct cgtctttccg cttcccggcc ctgctgccac
     1681 taatatatat tttgatacct tatcatttat aattcctaat tgagccggtg aaagctgccc
     1741 gaaaagctgt ctgaatcttt caggcgtcat atttctttgg atttcatcac ctttaggtcc
     1801 tttaaaatat tttcgcaaaa atgagctgta ttccaatcgg aaataatcat ctgcaaattt
     1861 aagcgcggaa ttgtagtctt ccatcatctt ttttgcatat tctcccacta tgtgtatcat
     1921 ttcaatctta tgtttgtaaa agtcttttag cgttttataa tcttcagcct tgtatctgat
     1981 tctattgtca tctatgagtc tttctatgct gatggcgttg tatattacaa aaaatccccc
     2041 ttcaatctta agtattccta tctttgaaag gtaaaaaagc gaatcttcta tttgccaggg
     2101 tgcagctttt ttattgaata gctgcatgtt gaaattatac tcacccatta attcgaggac
     2161 agaaaattcc aatgtatttt cattgtttac attaatctct tgaatgtgtt tcaatataaa
     2221 ttctgcaata gcccatcttg cctctaatat ctccaatgcc ttatctctgt cccttaaaag
     2281 atttatttta atatgattat tattcccttt ggcatttctt ttaacaagac cttttatcga
     2341 ccaaaaatta aaaatagttt taattttatc ggggttggaa ttttttacac ctgcatccag
     2401 agcaagttca tttaattgct tgatattttt agttgtagtt atctctgata tctgcgatag
     2461 gagaaagcgc tccaattcaa tatattcgta aagcttatca aaggattttt taccatagct
     2521 gctttcatcc ataaatatag tcaaatcctt tgcatcggat aatatcccag cttgtctcaa
     2581 aagctgaatt acatgtataa cctgtgcctt ttcaattccc aaatgatcgg aaatataatc
     2641 aaccctggat tctgcatctt cgctgcctgc tctgctccgg cttcttttgg atataagttt
     2701 gttcattatt ctcgcggctt gttgtttttc tgtctcacta aatatatcag agccggcaat
     2761 tttttcagaa acttccttca tatttcttgc cataatactg tctgcatata cccttggcat
     2821 attttgtcct cgtttaatgt aacctgcgtt ttccaatgct gcaactgctg tataaactct
     2881 tgtttccaag tcatacattc cttcatccca cccagctttc tttgcaattt caagtgctga
     2941 atttgacatg ttagatctaa acttagttgt attctttatg gcaatccaaa tttgctgaat
     3001 ttcttgtatg cttatttttg tttgatttaa aagaatgaaa tgtttgtcta aatctccatc
     3061 gttgaaaaga acatagcagc gagcatttat gctttggtct ctgcccgccc ttcctgcttc
     3121 ctgtacataa tcttccagtg agccgggaat atcgtagtgt actaccatcc ccacatcttt
     3181 tttatctaca cccatgccaa aggctgaagt tgcaaccatt atatccactt cgcccttaat
     3241 gaattcctct tgatttttac tcttttcttt tttatccatc ttcccatggt aaggtcttgc
     3301 ctcataaccg tcgtcagtaa gtcttttggc aagctccatg gcatttttta ttctggaaac
     3361 atatataatg gtaggacaat tgttgtattc gataaggttt cttaatgttt cgtatttttc
     3421 agaactttca ttcttgatta ttttataagt aaggtttttt cttcctgaat ctgctgtata
     3481 gagctttaat tccaaattga gattctcttt gaaatacttg caaatgtcgt ctattacatt
     3541 ttgttttgct gtagctgtaa agcatgatac cggtattatg taatcaagat ttttcttttc
     3601 gcagatcttt ttaataaatt ctgcaatata catataatca accctgaaat cctgccccca
     3661 ggctgaaaag caatgcgctt catcaattat aaatcttgca attttccgat cctgcaacag
     3721 cttttctatg gactttgatc tgagtgattc cggtgaaata tagagaatat gagcatcgcc
     3781 ggtctcaact ctctttatag cctcagctct ttctatggga tccaaaagtc cgttaatagt
     3841 tacggcatct gttatattga aattttccaa attatcgacc tgatctttca ttaaagattg
     3901 aagaggtgat attataacgc taagagattt tgtatttctt cctgccataa gtgcaggcac
     3961 ctggaatgtg attgatttgc ctccgcctgt ggggaaaact gccaaaagag aatctccgtt
     4021 tattgcgctt ttaactgctt gctcctgcaa aggaactccg tcaaaacttc tatatgcatc
     4081 ataaccgaaa aacctattca gcccctttac tgcatccatt gtttcattgc agtactcgca
     4141 gcctggaatg cactttgttc cccgcagctg attcattaca ttgtccacct ggggatatgt
     4201 cataagtacc caaggaggag taatggaata ctcgtcgtct gcacatatga gagacaggca
     4261 ataggcaagc tctattggat gagtataaat aatattttta atatctgaat tctcgcataa
     4321 cttgccgtag aaaaactttc ttatacctgc ttctgcttct aaatttgttc cttcattttt
     4381 gtaatcaata tatttaaaga aatctttaaa ctccttttta tcattcaaca atatataaag
     4441 tatccttttc atttcatcag gcagtaaatt aaaggctcct gtttcatcat agaaaaggtt
     4501 catggctttc ttacaatcgc taacaggatt gttaatctct tcagtcagca gcttatcgtc
     4561 ctttaccaat gaatgatagg gtctttgagg aaataaaaga gcagataaat gcaaggtgtc
     4621 tataacacca gcacatttaa tctgccctaa atatttattt aagtatttta aatcatgtgc
     4681 aattatatta tgtccacata aaaagtctgt ttctaaaagg aatcccctga tgacatcagg
     4741 tgaagcacca tgaatatatt ctccgtttgg tcttatagca ccaaagtcgc taatcttacc
     4801 actttccggc actacttctg catcaataaa tgatatctgc ttcataatac ccgccatctt
     4861 aagttgaata atatgtattt agctcattat atagcatatt gaaaataatt tcaatattga
     4921 tatgacaagg agatggggat tactttaggc gttcttctat cagttttgcc agctgggcgg
     4981 catggaagtt tatttctt
//
LOCUS       DAOSHP010000111         4772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_15960_length_4772_cov_9.368031, whole genome shotgun sequence.
ACCESSION   DAOSHP010000111 DAOSHP010000000
VERSION     DAOSHP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15960_length_4772_cov_9.368031"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1057
                     /locus_tag="PLB33_08560"
     CDS             <1..>1057
                     /locus_tag="PLB33_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63617.1"
                     /translation="DMHQINSIDKVDRFIDYIRSNERQLPFVLTVEYKNPVDFNTLVK
                     TKPNFDINNLLNRKIEDLIATTKVEDKEEIFEDRMRELAGKKMGYAQFGFLPYRYINN
                     FNDKLGCNISGGDVCVFYPERYNRKNLFKRDSIKLNEKKFLKDLETELQEFPKYKNIN
                     FGNVIFINEARIEELNKIIELSNSKEEIIKAIEDKIEIIEQKYEDKIAVLNNEMHEKD
                     EKIDTLKAEIKELNDKFDKFYLQVEKLKSDYNDQINKLNLKIEWKDNKLKQPRKTEDI
                     PEWVEKQFAGKLIFHNRAIGEIKKTPTGEIDMSLLCDAIEYLANEYRDELLGLISEVE
                     SNNICSDKYNRSFVVAPT"
     assembly_gap    1058..1157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1185..2051
                     /gene="panB"
                     /locus_tag="PLB33_08565"
     CDS             1185..2051
                     /gene="panB"
                     /locus_tag="PLB33_08565"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOG63618.1"
                     /translation="MAKKKTILDLYKMKENGEKAVWITAYDCCFAAYAEKAGMDMILV
                     GDSMGMIVYGYQGTTPVTMDMCINHCQAVRRGAPNTYIIGDMPFGSYHESVEDAVRNA
                     VRFIKEADVDAIKLEGGVSVADKIKAIAQAGIVVFGHVGLTPQSSAALGGFKAQGRTT
                     QSARAVIEDAIAVYEAGAKFILLEGVPEEVCAFVHKILPIPVYGIGAGADTDGQVLVI
                     GDALGMFEAFTPKFTKKYADIAGIATKAFADYVKDVKEGIFPAPEHKYKISGDMAEFE
                     KLFDEIEKDIKF"
     gene            2149..3345
                     /locus_tag="PLB33_08570"
     CDS             2149..3345
                     /locus_tag="PLB33_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG63619.1"
                     /translation="MKYDFTSIIDRRGMDSVAVDLRPGSGPEAPKEGFDVIPMWIADM
                     NFPTVPTIQEAIIERAKHPMFGYFLPKEEYFDSIIKWHEVRNGVTGLTKEHIGYENGV
                     LGCAMSAVNAFSAPGDCVLLHSPTYIGFTSSLENNGRKIIHSDLVRDEAGVWRMDYDD
                     MDAKLKKYNIHVAVFCSPHNPCGRVWERKEIEKAMEVYKRNDCVVISDEIWSDIILYG
                     NKHIPTQSVNEDAKNRTIALYAPSKTFNLAGLVGAYHIIYNKYLRDRVRAQGSKSHYN
                     ELNIFSMHALIGAYKKEGYEWVDELCQVLGENIDYASEHIINNYHGIKFFKPEGTYMM
                     YLDCEEWLKEHNMTLYDLYKKGTDVGVMWQDGRPFNHPYAIRLNLALPKSKVIEAFER
                     LDKYVF"
     gene            complement(3441..3911)
                     /locus_tag="PLB33_08575"
     CDS             complement(3441..3911)
                     /locus_tag="PLB33_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63620.1"
                     /translation="MKYYSNPNKYRNSYINKFNMNNNSNNEGTGFLEITVSDAQTGMP
                     VANVDIEVFKMTIMGEYAERAVSELVVRYSTFEDGTIPLIELPVIEWPLNRYFARLDT
                     FGYYDVSIVNIPIYDGVKTIYEVKLNRITSPTRIREYIRTPTRTEYYTPPVWFY"
     gene            complement(4191..4673)
                     /locus_tag="PLB33_08580"
     CDS             complement(4191..4673)
                     /locus_tag="PLB33_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOG63621.1"
                     /translation="MNKNKLTALILIMLFVLSPVAFAFGTITASNPGYEERVVQLVNA
                     ERARYGLDALYMDSRISDVARLKSKDMADLNYFSHYSPTYGMASDMLLRFGINYSAWG
                     ENIASGQDTPEIVVSQWMNSPSHRENILSPYFTFIGVGYYVGADGTPFWTQMFTSDDK
                     "
BASE COUNT         1583 a          693 c          958 g         1438 t
ORIGIN      
        1 gggatatgca tcaaatcaac agcattgata aggttgatag atttattgat tatatcagaa
       61 gcaatgaaag acagcttcct ttcgttttga ctgtcgaata taaaaatccg gttgatttca
      121 atactttagt aaaaaccaaa ccgaattttg acattaataa tctattgaac cgcaaaattg
      181 aagatttaat tgcaactacg aaggttgaag ataaagaaga aatatttgaa gatagaatga
      241 gagaattggc aggtaaaaaa atggggtatg cgcagtttgg atttttgcct tatagatata
      301 ttaataattt taatgataaa ctcgggtgca atatttcagg cggtgatgta tgtgtatttt
      361 atcctgaaag gtataatcgt aaaaatttat ttaaacgtga tagtattaaa ttaaatgaaa
      421 agaaattctt aaaagattta gagactgaac tgcaggaatt tccaaaatac aaaaacataa
      481 atttcggcaa tgtaattttt ataaatgaag caagaatcga agagcttaac aagattattg
      541 aattaagcaa ttcaaaagaa gaaattatta aagctattga agataagata gaaattattg
      601 agcagaaata tgaagataaa atagctgtct taaataatga gatgcatgaa aaggatgaga
      661 aaattgacac attaaaagca gaaataaaag aacttaatga caagtttgat aagttttatc
      721 tgcaagtaga gaaacttaag agtgattaca atgaccagat taacaagctg aatttaaaaa
      781 ttgaatggaa ggataataaa cttaagcagc cgaggaagac ggaagatata cctgaatggg
      841 ttgaaaaaca atttgccgga aaacttattt ttcacaacag ggcaatcggt gagattaaaa
      901 agactcctac aggtgaaata gatatgagtt tgctttgtga tgctattgag tatcttgcaa
      961 atgaatatcg cgatgaacta ttaggtttaa tcagtgaggt ggaaagcaat aatatttgtt
     1021 ccgataaata taacagaagt tttgttgtag ctcccacnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnaaa ctatgatgaa agaggggtgc cgatatggct aagaaaaaaa
     1201 ctattttaga cttatataaa atgaaagaaa atggtgaaaa ggcagtatgg attactgcat
     1261 atgactgttg ctttgcagca tatgcggaaa aagccggcat ggatatgatt ttggtgggag
     1321 attcaatggg aatgatagtc tacggatatc aaggcactac tcccgttact atggatatgt
     1381 gcattaatca ttgtcaagca gtaagaagag gagctcccaa tacatatata atcggtgata
     1441 tgccatttgg ctcatatcat gaaagtgttg aggatgctgt gcgtaacgca gtacgtttca
     1501 taaaagaagc tgatgttgat gccataaaat tagagggcgg cgtttctgtt gcagataaaa
     1561 ttaaagctat agcacaagca ggaatagttg tatttgggca tgtcggatta actcctcaaa
     1621 gttctgctgc tttaggcggg tttaaggctc aggggcgtac aacgcaaagt gcacgtgctg
     1681 taattgaaga tgccatagct gtttatgaag caggagcaaa atttatattg ttggaaggtg
     1741 ttcccgaaga ggtctgtgct tttgttcata aaatattgcc tataccggtt tatggaatcg
     1801 gggcaggagc tgatacggac ggtcaagtat tggttatcgg cgatgctctt ggaatgtttg
     1861 aagcatttac acctaaattc acaaagaaat atgccgacat agcaggcatt gcaacaaaag
     1921 cctttgcgga ttatgtcaaa gatgtaaaag aaggaatttt cccggcacct gagcataaat
     1981 ataaaatatc cggagacatg gcagagtttg aaaagctgtt tgatgaaata gaaaaggata
     2041 ttaaattttg atttataatt tgaaggatgt cagacgatgc atcagcacgc ggcatttttg
     2101 tcatgtgtaa gaacaagtca tactaaatta atgaggggaa tattaaatat gaaatatgat
     2161 tttacaagta ttatcgatag acgcggcatg gattccgttg ccgttgattt aagacccggt
     2221 tcgggaccgg aagctccaaa ggaaggattt gacgtaattc caatgtggat tgcagatatg
     2281 aattttccta cggttcctac cattcaggag gcaattattg agcgtgcaaa gcatccgatg
     2341 tttggatatt ttttgcctaa agaagagtat tttgattcaa ttataaaatg gcatgaagtt
     2401 cgaaacggag taacaggatt gaccaaagaa catatcggtt atgaaaatgg tgtgcttggt
     2461 tgtgcaatgt ctgcagtgaa tgcattttct gctccggggg actgcgtgct tcttcattca
     2521 cctacttata taggatttac atcatctttg gaaaacaacg gccgaaaaat tatacattct
     2581 gacctcgtac gggatgaagc cggagtatgg cgtatggatt atgatgatat ggatgcaaag
     2641 cttaagaaat ataatattca cgtagctgtt ttttgttctc cgcacaatcc atgcgggcgc
     2701 gtatgggaga gaaaagaaat tgagaaggca atggaagtat ataaaaggaa tgattgtgtt
     2761 gtaatttccg atgaaatttg gagcgatatt attctctacg gaaacaaaca tataccaaca
     2821 cagagcgtaa atgaagatgc taagaatcgc accattgctc tttatgctcc cagcaagacg
     2881 tttaatcttg ccggtttagt cggagcttat catatcattt ataacaagta tctgcgtgac
     2941 cgtgtcagag cccaaggcag caagtctcat tacaatgagc tcaatatttt ttctatgcat
     3001 gcattgattg gagcttacaa aaaggaaggt tatgagtggg ttgatgaatt gtgtcaggtg
     3061 cttggtgaaa atatagatta tgcaagtgag cacattatta ataactacca tggaattaaa
     3121 ttttttaaac ctgaaggaac ctacatgatg tatcttgact gcgaggagtg gttaaaggag
     3181 cataatatga cactttacga tttgtacaaa aaaggtaccg atgtaggtgt tatgtggcag
     3241 gatggacgcc ctttcaatca tccctatgcg attcgtttaa acttagcatt gcctaaatca
     3301 aaagtaatag aagcttttga gcgtttggat aaatatgtgt tttgacataa tagggacaca
     3361 cctctactac ttggaatagc tttggtaatg gaggaatgtc ccttatttgt taaatctatt
     3421 gctgtttctt atgaaatcac ctagtagaac caaacaggag gagtgtagta ctctgttctt
     3481 gtgggagtcc ttatatattc gcgtatacga gtaggagaag taattctgtt caatttgact
     3541 tcgtatatag ttttaacacc atcatatatg gggatgttaa ctatggaaac atcatagtat
     3601 ccgaaagtat ccaaacgtgc aaaataccgg tttaagggcc attcaattac aggcagctct
     3661 attaagggaa ttgtaccatc ctcaaatgta gaatacctaa ctactaattc tgatacggct
     3721 ctttcagcat attcacccat tatagtcatt ttaaacactt caatatctac attggcaaca
     3781 ggcatgccgg tttgagcatc tgacacggtt atttctaaaa aacctgttcc ttcattattg
     3841 gaattattat tcatattgaa tttatttata tatgagtttc tatatttgtt aggatttgaa
     3901 tagtatttca tttttaacct ccattcatca taaccgatgc aacttgcttt attatatgta
     3961 aaggttgtaa ttaagttatg aattaaatat agataggaat taagacaagt tgaataagaa
     4021 aaaatacatt ttaggaaggg tagcactaaa atgtattttt ttggatttag gtgttattta
     4081 ttaaattata tatattaaca aaatggtttt caatcaccct tagggtgatt tttcctaaaa
     4141 aatacgttct ttaaaaaaag aacgtatttt ttggcgatta gaacacctgc ttatttatcg
     4201 tcactggtaa acatttgagt ccagaatgga gttccgtcag caccaacata atatcctaca
     4261 ccgataaaag taaaataagg tgaaagtata ttttctctgt gagacgggga attcatccat
     4321 tgagaaacta caatttcagg agtatcctgt cccgaagcta tattttcgcc ccaggcagaa
     4381 taattgattc caaatctaag cagcatgtcg ctagccatac cataggttgg tgaatagtga
     4441 ctaaaataat ttaaatcagc catatctttt gattttagcc tggctacgtc tgaaattcta
     4501 ctgtccatat acagtgcatc caatccgtat cttgctcttt cggcattaac taactggacg
     4561 actctttctt cataaccggg gtttgatgcc gttattgtac cgaaagcaaa tgcaaccggt
     4621 gacaatacaa agagcataat caaaatcaat gctgttaact tatttttgtt catacaacct
     4681 cctgttctga aacaaaaaaa tttgtttcaa taatattgtt tcaatttatt cataatatat
     4741 aatttcatat atttgatagg ttgtatattt gt
//
LOCUS       DAOSHP010000112         4580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_16897_length_4580_cov_9.410387, whole genome shotgun sequence.
ACCESSION   DAOSHP010000112 DAOSHP010000000
VERSION     DAOSHP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16897_length_4580_cov_9.410387"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLB33_08585"
     CDS             <1..1221
                     /locus_tag="PLB33_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOG63622.1"
                     /translation="LERSWILYDVGNSAFILLISTIIPIYFKNIASSDGVLPSDSTAY
                     WGYAMSTSTLIVAILGPVLGALADTKGYKKPLFTFFLMSGVLGCAALSLPVPWIIFLA
                     LFVFARVGISGSLIFYDAMLPDVTSDERMDLLSSHGYAWGYIGSCIPFTISLVLILFA
                     DKIGIATALATGIAFILNALWWFLVSLPLLKNYKQLHYVEVDNAPMAMAFKRLGRTFG
                     DIKKHKEIFIFLLAFFFYIDGVDTIIGMATSYGKDVGISDNNLLLALLLTQVVAFPFA
                     IIFGKLSKRYKTSELISVCIIGYFLITLFALQLDKAWEFWFLAVCVAVFQGAIQALSR
                     SYYAKIIPKESSSEYFGFYDIFGKGASFTGTTLMGITTQITGNSKFGVLTICIMFIIG
                     YVIFRKAVKMQTVS"
     gene            1283..2668
                     /gene="amrA"
                     /locus_tag="PLB33_08590"
     CDS             1283..2668
                     /gene="amrA"
                     /locus_tag="PLB33_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOG63623.1"
                     /translation="MMIVNSFIVPHPPLIIPEIGRGEEIKIQKTIDAYHNIAKQISEI
                     KPDTIIITTPHSIMYSDYIHISPGKRAKGSFREFGQRDVTMEVEYDVELVGEISNEAE
                     KAGISAGTLGEKNSSLDHGVMVPLYFVNKYYNNYKLVRISISGLSYLEHYKFGKCIKK
                     AAEKSNKKIVFIASGDLSHMLKETGPYGYREEGVIFDREVTKAMDSGNFSEFLKFDED
                     FCEAAAECGLRSFIIMAGALDGKKLSSKLLSYQGPFGVGYAVAEFKVMGDDEGRRFDE
                     IYLKTEMERIESLKSEEDEYVRLARQTLESYVINRKVIKRPDNLSPELIKNKAGVFVS
                     LKLDGSLRGCIGTISPTTSSIADEIIRNAISAGTEDPRFPPVTEDELSRLEYSVDVLG
                     KAEKIESMDELDPLRYGVIVSKGHRRGLLLPNLEGVETAEKQISIALRKAGISPNEDY
                     QLERFEVVRHK"
     gene            2665..3495
                     /gene="amrS"
                     /locus_tag="PLB33_08595"
     CDS             2665..3495
                     /gene="amrS"
                     /locus_tag="PLB33_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOG63624.1"
                     /translation="MSKIICNICPHHCNLNEGQTGLCRARKNINGEITAINYGQLTAL
                     ALDPIEKKPLNRFYPGSNILSVGSFGCNLKCLFCQNYHISMASKNEVKTQFVKPEELI
                     NIALDLKDRKNIGIAYTYNEPLVGYEYVLDCAKLARDKNLKNVIVTNGCFCEEPMKKL
                     LPFIDALNIDLKGFTYEFYKKIGGDLDTVKNFIKLAAKHCHVEITTLIIPGENDTEDE
                     IRELSEFIFSVSPEIPLHVSRFFPCYKMLDKRPTDVKKVYDLANIAREKLKYVYEGNC
                     "
     gene            3590..4405
                     /locus_tag="PLB33_08600"
     CDS             3590..4405
                     /locus_tag="PLB33_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOG63625.1"
                     /translation="MNFMSGFLKGLALGIAAIAPGISGGALAVIFGLYDKITYFIAHF
                     TRNFKENVLYFMPVGIGGVVGVLIFSRIIEYLFHFHEVEVKYAFIGLMFGTIPLVVKE
                     ANKKGYKQRYLIPFIITLSITLVAAYFEKDEINIIMNSGTAPLSLIIYGIIIGFGTII
                     PGISASILLMYMGAYEIVISAISNIQISIIFFLAIGFILSILLFSKIICMLFEKAYGI
                     TYYAILGLVLGSVLSIFPGFYLNFDYLLNLIILLACCLLSYSLSKLGKGKTIS"
BASE COUNT         1499 a          700 c          953 g         1428 t
ORIGIN      
        1 ttggaaaggt cctggatttt gtatgatgtc gggaattctg catttattct gctcatatca
       61 acaatcatac caatctattt caaaaatatt gcttcatcgg acggagtttt accttccgat
      121 tcgactgctt attggggata tgccatgtcg acttcaactt tgatagttgc gatattagga
      181 cctgtattag gagcattagc agatacaaaa ggatataaga aacctttgtt tacctttttt
      241 ttaatgtccg gggtacttgg gtgcgcagca ctatcacttc ccgttccgtg gataatattt
      301 ttagccttgt ttgtatttgc aagggtaggt atttcaggaa gtctgatatt ttacgatgcc
      361 atgcttccgg atgtaacaag tgatgaaagg atggatttat tgtcttccca cggttatgcc
      421 tggggttata taggaagctg tattcctttt accatcagtc tcgtgttgat tttatttgca
      481 gataaaattg gaattgccac cgcccttgca acgggaatag cttttatatt aaatgcatta
      541 tggtggtttt tagtaagcct gcctcttctt aaaaattaca agcagcttca ttacgtggag
      601 gtggataatg ctcccatggc tatggcattt aagcggcttg gaagaacttt tggcgatata
      661 aaaaaacata aggaaatatt catatttttg cttgcctttt tcttttatat agacggggta
      721 gatacaatta taggaatggc cacatcatac ggcaaggacg ttggaataag tgacaataat
      781 ctattattgg cgcttctttt aacacaggtg gttgcttttc cttttgcaat aatatttggc
      841 aagctgtcta aaaggtataa gacttcagag ctaatcagcg tgtgcatcat aggttatttt
      901 ttaattacac tgtttgcgct gcagcttgat aaagcttggg aattttggtt tctggcagta
      961 tgcgtagcgg ttttccaggg agcgattcaa gctttgtccc gctcatatta cgcaaaaata
     1021 ataccaaagg agagttcaag cgaatatttc ggcttttacg atatttttgg taaaggtgca
     1081 tcctttaccg gaacaacgct tatgggaatt acgacacaaa ttacgggcaa ctcaaaattc
     1141 ggtgtattga ccatttgtat tatgttcata ataggatatg taatattcag aaaagcagta
     1201 aaaatgcaga ctgtctcatg agatagcttt tggaatgaca tatgttattc atagcaatca
     1261 tataagaaag aaaggagaaa ttatgatgat tgtgaatagt tttatagttc cgcatcctcc
     1321 attgataatt cctgagatag gaagaggaga agaaattaaa attcaaaaga caatagatgc
     1381 atatcacaat attgcaaaac aaatttctga aattaaacct gacacaataa ttataacaac
     1441 tccccattca ataatgtatt cagattatat acatatttca ccggggaaga gggcaaaagg
     1501 cagcttccgg gaatttggcc aacgtgatgt tacaatggaa gtagaatatg atgttgagtt
     1561 agtcggtgaa atttcaaatg aagcagagaa agctgggatt tctgccggaa ctttgggtga
     1621 gaaaaacagc tcgcttgacc acggtgttat ggtgcctctt tatttcgtga ataaatacta
     1681 caataattat aagttggtgc gcatttccat atcgggactt tcttatttag agcattataa
     1741 atttggaaaa tgcattaaaa aggcagcaga aaaaagcaac aaaaaaattg tttttatagc
     1801 aagcggggat ttgtctcaca tgcttaagga aacaggccct tacggttaca gggaagaggg
     1861 agtgatattt gacagggaag taaccaaggc aatggatagc ggaaatttct cggagtttct
     1921 taaatttgac gaagatttct gtgaagctgc tgctgaatgc ggacttcgct ccttcataat
     1981 aatggcaggc gcattggacg ggaaaaaatt aagctcaaag cttctgtctt atcaaggacc
     2041 ttttggagta ggctatgcag ttgcagaatt taaggttatg ggagatgatg aagggcgtcg
     2101 atttgatgaa atatacttaa aaaccgaaat ggaaaggatt gagtcattga aatcagaaga
     2161 ggatgaatat gtgcgccttg cccgccaaac attggaaagc tatgtaataa accgaaaggt
     2221 aattaagaga ccggacaatt tgagccctga acttattaaa aacaaggcag gagtatttgt
     2281 atccttgaaa ctagacggca gcttaagagg ctgcattgga acaatttctc cgacaacatc
     2341 aagcatagct gatgaaataa taaggaatgc aataagtgcc ggtacagaag acccgaggtt
     2401 tccgcctgtc actgaagatg aactctcaag actggaatac agcgtagatg ttctcggtaa
     2461 agcagaaaaa atagagtcaa tggatgaact tgatccccta agatacggtg tcattgtttc
     2521 aaaaggacat agaagagggc ttttattacc caatttggag ggggttgaaa cagctgaaaa
     2581 acaaatctcc atagctttga gaaaagcagg tatttctcca aatgaagact atcagttgga
     2641 aagatttgag gtggtaaggc acaaatgagc aaaataattt gcaatatatg cccgcatcac
     2701 tgtaatttaa atgaaggtca aactggttta tgcagggcaa ggaaaaatat taacggtgaa
     2761 attacggcaa tcaattacgg tcaattaaca gctcttgccc ttgacccaat cgaaaaaaag
     2821 cctttgaaca ggttttatcc cggcagcaat atactgtctg taggaagctt cggatgcaac
     2881 ttaaaatgtt tattttgcca gaattatcat atatccatgg catccaaaaa tgaggttaaa
     2941 actcaatttg taaaacctga agaattaata aatatagcat tggatttaaa ggacagaaaa
     3001 aatataggca tagcatatac ctacaacgag cctttggtag gatatgaata tgttttagac
     3061 tgtgctaaat tggcaagaga taagaattta aagaatgtga ttgttacaaa cggttgtttt
     3121 tgtgaagagc cgatgaaaaa actacttcct tttattgatg ctctgaatat tgatttaaaa
     3181 gggtttactt atgagtttta taaaaaaatc ggcggggact tggacacagt taaaaatttt
     3241 attaagcttg cagcaaaaca ttgtcatgtg gaaataacaa ctcttataat acctggtgaa
     3301 aatgacacgg aggatgaaat aagggagctc tcggaattta tattttcagt gtcgcctgaa
     3361 atacctcttc atgtttctag gtttttccct tgctacaaga tgcttgataa aaggccgaca
     3421 gacgtaaaaa aggtttatga tttggcaaat atagcaagag aaaaactcaa atacgtctat
     3481 gaaggaaact gctgaaagat ttggtgaatg agactgcgta cgatttttta ttgctaaaat
     3541 taccatatgg acatataatt aaacaagagt atactggcag gtgtgtgtga tgaattttat
     3601 gtcgggattt ttaaagggtt tggcattggg cattgcggct atagctcccg gaatcagcgg
     3661 aggagccttg gctgtaatat ttggtttgta tgacaaaatt acatacttta ttgctcattt
     3721 taccaggaat tttaaggaaa atgtattgta tttcatgcct gtcggtattg ggggagtcgt
     3781 tggagtatta attttcagca gaattattga ataccttttc cattttcacg aagtcgaagt
     3841 aaaatatgct tttataggct tgatgtttgg taccatacct ctggtagtaa aagaagcaaa
     3901 taaaaaaggc tataaacaaa gatatttaat accttttata ataaccctga gcattacatt
     3961 agttgctgca tattttgaaa aagatgaaat taatataata atgaactcag gaaccgctcc
     4021 tctgagcctg attatttatg gcatcataat tggatttgga acaattatac ccggtatcag
     4081 cgcttctata ctgcttatgt atatgggtgc ttatgaaata gtcataagtg caatttcaaa
     4141 tattcaaata tccataattt tttttctcgc cataggcttt atactgagta tattgttatt
     4201 ttcaaaaata atatgcatgc tttttgaaaa ggcatatgga attacatact atgcaattct
     4261 aggccttgta ttaggttccg ttctttcgat attccctggt ttttatttga attttgatta
     4321 tttattaaac ttaataatac tattagcatg ctgtttactg tcatattcat tgtcaaaatt
     4381 aggaaaagga aaaacaattt cttaagttgt ttatactttt actaggagag ttggaaattg
     4441 ttacaaaatc ttttcattta attgaaaaat aaacaatatt ttttaactta ccttgactgg
     4501 ataacagctt agtgatacaa taaaatatga aaaataggga ggtgaaatac aagggtattc
     4561 cggtaaaagg aagtactcaa
//
LOCUS       DAOSHP010000113         4530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_17175_length_4530_cov_11.843352, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000113 DAOSHP010000000
VERSION     DAOSHP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17175_length_4530_cov_11.843352"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLB33_08605"
     CDS             <1..586
                     /locus_tag="PLB33_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63626.1"
                     /translation="TNILQVIDMVFNEKTITLKNGERCLLRSPEAKDAAEMLNYLKKS
                     AAETDFLLRYPEEVVMTIEEEVNFINFLREAPRGVMICAFINNKLVGTTHLMAIGERF
                     KIFHRASFGIAVLKEAWHIGIGNALITEILKIAEDIGFELVELEVASPNHNAIGLYKK
                     FGFKIYGTRENGFKFKDGTYCDEHLMMRKIGSRI"
     gene            819..1052
                     /locus_tag="PLB33_08610"
     CDS             819..1052
                     /locus_tag="PLB33_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOG63627.1"
                     /translation="MGDHHNNDKPSKKYSDMFAALAAFSQIGFTIAVSVLGGVLLGQY
                     LDDKFGTDPWFLLAFSILGVGVGIRALFNIAKK"
     gene            complement(1233..1820)
                     /locus_tag="PLB33_08615"
     CDS             complement(1233..1820)
                     /locus_tag="PLB33_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HOG63628.1"
                     /translation="MNSHILYNQALHAGALNTNTSLMNYSVNIPISLMKSIEGKYFVG
                     IAPNLQFGNATKAWARLYNPPNSGVNLFVNTWTVSDIFSTPYSVQIWFNSTPPGLIQF
                     SRSVTPSNLAISPPPQSNIQLQYAISVSGFPAVGVKAYSRYGLAGTTINSEEEGKFIF
                     APGGSYLIFLSNPERPTIPASGNIAFGWWEEPIYQ"
     gene            2216..2794
                     /locus_tag="PLB33_08620"
     CDS             2216..2794
                     /locus_tag="PLB33_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63629.1"
                     /translation="MSNLVTSIGIIAGIFYIIYVIMDFMADNFIYEKQNILLMLVMAI
                     LMNIAGFMHSRYYYYDKTSTMLVNIAVVTALLGGYLVFQRKRIYYFRGIDNKLINKYK
                     NEITKIIEDYKADNLGRKADINFINNKLIFSKVSKDKTKKCLSLIGTFLDENRREYKL
                     KDYLAYFIRGHLFPAVTAILIFFIFYKLMQLI"
     gene            2815..3573
                     /locus_tag="PLB33_08625"
     CDS             2815..3573
                     /locus_tag="PLB33_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOG63630.1"
                     /translation="MDNKDYLTRIENNRELMKSVFTERGHKSDQQSQVPQPPLAKESV
                     NENKMVLTKDFSGLIKESNVLTIINERKSHRVFKKESLTLDHLSFLLWASQGVKGIKG
                     DNYATERTVPSGGARHAFEVYLAAINVKGLEKGIYHYLPLNHELELIRLVDNIENAVA
                     ESLDDQKWAGKSAAVFYYTAIPYRSEWRYTINAAKLILLDAGHAVQNLYIGCGAIGCG
                     TCAVADYNQNLSDELLQVDSKDEFVVYAAPVGLI"
     gene            3640..>4530
                     /locus_tag="PLB33_08630"
     CDS             3640..>4530
                     /locus_tag="PLB33_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOG63631.1"
                     /translation="MIEIGVSNLTKAYGADVIFENVTFDVKTKDRIGLLGRNGTGKTT
                     LMKIMAGFEDYQKGQISKRKCLSIGYLEQIPNYEDDSIVLDVLNEAFSQLNELKLQMD
                     ELEKSFESLKGDELKKAVDKYSSIHDTYELGGGYNTKEKLNRILFGLEIPDDMQKRQF
                     NQLSGGEKTRVILGKILLEEPELLLLDEPSNHLDIKSVEWLEEYLGSYGGTMVIVSHD
                     RYFLDRVVNKIIELESDGVEIYHGNYSKYVAEKELRFLLAMKEYENQQRKIKKMEEQI
                     QRYRIWGEMRDSDKMYKRAKE"
BASE COUNT         1628 a          649 c          945 g         1308 t
ORIGIN      
        1 tactaatata ttgcaggtga ttgatatggt attcaatgag aaaactatta cattaaaaaa
       61 tggtgaaaga tgtttattaa ggagccccga agctaaagat gcagctgaaa tgcttaatta
      121 tttaaagaag tcagcagcag aaacggattt tttgcttcgt tatcctgaag aagtagtaat
      181 gacaattgaa gaagaagtaa atttcataaa ttttttaagg gaagctccaa gaggtgtaat
      241 gatttgtgct tttattaata ataaacttgt tggaaccact cacttaatgg ctatcggtga
      301 aaggtttaaa atctttcata gagcatcttt tggcatagcg gttctaaagg aagcatggca
      361 cattggaata gggaacgccc tgataactga aatattaaag attgcagagg atatcggttt
      421 cgaactggtt gagctggagg ttgcaagtcc aaatcacaat gcaatagggc tttataagaa
      481 attcggattt aaaatatacg gcacaaggga aaacggcttt aaatttaagg acggcacata
      541 ttgtgacgag catttgatga tgagaaagat aggaagcagg atttaaaata tattactggt
      601 aaaatttcat atgaacagga agtagatatg gcaggcgatg attcaaaata taccttcctg
      661 accacctttc ggaaaacgaa gaatcctatg ttttattgca tgaacagatt ctaaaacttg
      721 acattataac gacatatatg ttaagatttt agagagcaaa aatggtgtcc gacttatttc
      781 aacaggacac tatgttttta tcgaggagga tcctatattt gggcgaccac cacaataatg
      841 ataaaccatc aaaaaaatac agtgacatgt tcgctgcctt agctgccttt tcacagatag
      901 gatttacaat agcagtatcg gtactgggcg gggtattact gggacaatat ttagacgaca
      961 aattcggtac agacccatgg tttcttttgg ctttttctat tttaggcgtt ggggttggaa
     1021 tcagggcctt atttaatatt gccaagaaat aaaaatgaca aatcgtaaga ttacgggaag
     1081 tggagaagta ttttggggac ggtttttttc gttctcagat ttttttcagg atgttttctc
     1141 aaagaaacgt agacacaaca aaagaagtat ggcgacacag acataccaat cataatttat
     1201 ataagaataa ttttcagtta aacaataagc ggctattgat aaattggttc ctcccaccat
     1261 ccgaatgcaa tattacctga agcaggtata gttggtcttt cggggtttga taaaaaaatt
     1321 aaatatgaac cgcctggagc aaatataaac tttccttctt cctctgaatt tatggtagta
     1381 ccggcaagac catatctgct gtaagcctta actcctactg ctggaaaacc actgactgaa
     1441 atagcatatt gaagctgtat attagactgt ggaggcggag aaattgccaa attagatggg
     1501 gtaacagatc gggaaaattg gattaatccc ggcggtgtac tgttaaacca aatttgtaca
     1561 ctatatggtg ttgaaaagat atcacttaca gtccaagtat ttacaaataa attgacaccg
     1621 gaatttggcg gattgtatag ccttgcccat gctttggtgg cattgccaaa ttgcaggttt
     1681 ggggcaattc cgacaaaata ctttccctca atagacttca ttaagcttat aggaatatta
     1741 acactgtaat tcatcaagct ggtattagta tttaaagctc ctgcatgtag agcttggtta
     1801 tataatatat ggctattcat taaatttgca acctttcata tcatagttat ttattatatg
     1861 atagaagtat atgaaaggtg aattcagagt tttagggatg gtttggtcaa gactcctttt
     1921 ggtatacact ttgggaacga ttctaaacgt taaaagaata atcttatatt aaattaagta
     1981 acgggaacgc gctagtaccc gttttttatt gaaataagga acaattttat gttaatttag
     2041 tcatttgatg tggtataatg taattattaa cagaaagctg tttgcgtctt tagtatgaat
     2101 aaccttaaaa ttaattagag acgacagaaa taaacatcct aaattaagcg taataatctt
     2161 ttaagaacaa aagaaccgtc ccaaaaaaga ggcaaggtaa ggaagggttg aagatatgag
     2221 taatttagtg acatcaatag gaataatagc aggaatcttt tatattatct acgtgataat
     2281 ggactttatg gctgacaact tcatatatga aaaacagaat atcttgttaa tgttagtaat
     2341 ggcaatactg atgaatatag caggctttat gcacagccgt tactattatt atgacaaaac
     2401 ttcaacaatg ctggtcaata tagcagtggt tactgcacta ttgggcggct atttggtttt
     2461 ccaaagaaaa agaatctatt atttcagggg aatagacaat aaactaatta ataaatataa
     2521 aaatgaaata acaaaaataa ttgaggatta caaagcagat aatttaggta gaaaggctga
     2581 tattaatttt atcaataaca aattaatatt cagtaaagta agcaaagaca aaactaaaaa
     2641 atgcttatct ctaatcggaa cttttttgga tgaaaacagg agggaatata aattaaagga
     2701 ctatttggca tattttatta gaggacacct tttccctgca gttactgcaa ttttaatatt
     2761 ttttatattt tataaattga tgcagttaat ataaaataaa gaaaggatta ttatatggat
     2821 aataaagatt acttaacaag aatagaaaac aacagagaat taatgaaaag tgtatttaca
     2881 gaaagaggac ataagtcaga ccagcagagt caggtgcctc agccaccctt agctaaagaa
     2941 tcagtcaacg aaaacaaaat ggtacttact aaagacttct caggacttat taaggagtca
     3001 aatgttttaa cgattataaa tgaaagaaaa agccaccggg tttttaaaaa agaaagtctt
     3061 acattagacc acctgtcatt tttattgtgg gctagtcaag gggtaaaggg aatcaaagga
     3121 gacaattatg caacagaaag aaccgtcccc tcaggaggag cgaggcatgc atttgaagta
     3181 tacttagcag ccatcaatgt gaaaggctta gaaaaaggaa tatatcatta tcttcccctt
     3241 aaccatgaac tagagttaat tagattagta gacaatatag aaaacgctgt agctgaaagt
     3301 ttggatgacc agaaatgggc aggaaaatca gcagcagtat tttattatac tgccatcccc
     3361 tatagaagcg agtggaggta tacaatcaac gcagctaagc tcatactttt agatgccggt
     3421 catgcagtcc aaaacctata tataggctgc ggtgccatag gctgcggaac ctgtgcagtg
     3481 gcagattaca accagaattt atccgatgaa ttattgcagg tagacagcaa ggatgaattt
     3541 gtagtatatg ctgccccggt aggattaata tagacttaag gagcattggg actgaatagt
     3601 actctagaat actaagcagc tgttaattat gaggtaatta tgatagaaat aggcgttagc
     3661 aatttaacca aggcatatgg ggcagatgta atattcgaaa atgtaacctt tgatgttaaa
     3721 actaaggata gaatagggct attggggaga aacggcacgg gcaaaacaac tttgatgaaa
     3781 attatggccg gatttgaaga ttatcagaag ggtcagatca gcaaacgaaa gtgtctgtca
     3841 attggttatc tggagcagat accgaattat gaggatgata gtattgtttt agatgtatta
     3901 aatgaagcat tcagtcaatt aaatgagctg aagctccaaa tggatgagct cgaaaagtca
     3961 tttgaaagct taaaaggtga tgagctaaag aaggctgttg acaaatacag ctctattcat
     4021 gatacatatg aattaggagg cggttataat actaaggaaa aattaaaccg gatactcttt
     4081 ggtcttgaaa tacctgatga tatgcagaaa agacaattta atcagcttag cggaggtgaa
     4141 aaaaccaggg ttattttagg aaaaatattg ctggaagaac cggagcttct gctacttgac
     4201 gaaccgtcaa atcatttgga tattaagtct gtggaatggc tggaggagta tcttggttca
     4261 tacggcggaa caatggttat agtatcccat gacaggtatt ttttggacag ggttgttaat
     4321 aaaataatag aattagaatc tgacggtgta gagatttatc acggcaatta ttccaaatat
     4381 gttgctgaaa aggaattaag atttcttttg gcaatgaaag aatacgaaaa tcagcagaga
     4441 aaaattaaaa agatggaaga acagatccaa aggtaccgca tttggggaga aatgcgggac
     4501 agtgataaaa tgtacaaaag agccaaggaa
//
LOCUS       DAOSHP010000114         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_18505_length_4300_cov_8.038398, whole genome shotgun sequence.
ACCESSION   DAOSHP010000114 DAOSHP010000000
VERSION     DAOSHP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18505_length_4300_cov_8.038398"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1409)
                     /locus_tag="PLB33_08635"
     CDS             complement(18..1409)
                     /locus_tag="PLB33_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOG63632.1"
                     /translation="MSIEIPGLTQVVIPAAITVHLGAPDEPGENVTVPFLDYIKNVAS
                     SELYPTWPEAALRANIYAITSTALNRIFTEWYRAQGYNFDITNDTRFDQAFVHNRGIF
                     DTVSVLADELFDSYIIRRGQIQPLYAQFCDGRVSWCPGLLQWGTVELAEQGYTPYEML
                     QYYYGEDIDIKTDTPIAEAFETYPGISLQLGDNNPYVLLMQIRLNTIATNYPAIQRIP
                     NPTGTFDEATQEAVRQFQDIFNFPVTGIIDKSTWYGIRRIYAAVTKLAELTSRGIMLS
                     EIPEFTPTPGPEEVIPRVQIVQYFLNVLSAYYNSIPSVDISGVLNTQTRTALMEFQKT
                     FNLPITGLVDEQTWNTMFNAVSGILETLPPSAIALPALLWPGITYRVGSEGPGVYLLQ
                     QYLSYIASVLEGITPTDPDGIFGPQTELAVRTFQEFFGIDVTGVVDRYTWDRIVLIYR
                     NLRFGDTSRVQGS"
     gene            complement(1420..1887)
                     /locus_tag="PLB33_08640"
     CDS             complement(1420..1887)
                     /locus_tag="PLB33_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOG63633.1"
                     /translation="MKYNPFDGRIYKAQVPPGSIPFEQEVGYLSVSVFTASGALPVEG
                     ALVTVYIRDESGAEHEIVHHITDANGRIPLITLPVMYDRLDPFESGRYYFTTYNLRVQ
                     AINYYTVNVIDLRIFPNTTTRFQINMIPVAIGPVEGPDTTIVIPPSPIDISND"
     gene            2076..3278
                     /locus_tag="PLB33_08645"
     CDS             2076..3278
                     /locus_tag="PLB33_08645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG63634.1"
                     /translation="MLARAFKLIKNLFSTSKTDDCKKEFAIDFCRINLAKLIISAKTL
                     IALGIIQLIVYCIVSKGNLFTKPQIYFGAMNFILLFAMTIFLSVIVKLEKDAAKNVVS
                     IHNAQIFFINFILLWSGGISLLHQLTHGQTIVYIAAVIITAGALYIEPSVSFLIYFTS
                     HLIFSVLLPNFQKSPDLLFGIYLNTTSLVIMSFAISYTRYKRQEQDFKNRKIMQENSD
                     ELKRINKELEEANKKLKIISQYDGLTGIFNRHTFDTKIREEWNRCKRHSMPLSLIMAD
                     IDHFKSFNDKFGHQAGDNCLKLVAEILSECAKRSSDVVARYGGEEFVILLPQTERERA
                     YDLAEKMRKRVEELNITISLGVNTTIPSDESSVREFIKNTDKALYIAKNKRNSTEVYL
                     PIDTFGFS"
     gene            complement(3249..4202)
                     /locus_tag="PLB33_08650"
     CDS             complement(3249..4202)
                     /locus_tag="PLB33_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOG63635.1"
                     /translation="MTKVKKAVYYICDNHDWGYVSYLVWDILKEEGYLNNKVDFTFDG
                     REVYKYIDNKNNEFYFVPTDIALCRDYPRYLPDMNRYFSDFDMSGMVTWHEGTNALDK
                     VITVHSIGDVNSGCYGPANPLYMHNLMWAMEKLRKEIELDFSVVTEATHWSGVYEGQG
                     DPELLLKYPVPIVDIEIGSTPESWVNMDACRVLALSLLHIFDKDIDDIKLHNLLCVGG
                     VHFDPNFAEAVHTCWNENEFFGVSHIIANQWLVSGEYEGENGIEKASECVDAIIGGID
                     AIVFHDKLKGCYKDVVRALGEKYKVPILKHQALRKPESINW"
BASE COUNT         1349 a          814 c          780 g         1357 t
ORIGIN      
        1 ccggtggttt tgacatttta gcttccttga actctgcttg tatcgccgaa tcttaaatta
       61 cgatatataa gtactattct atcccaagta tatctgtcaa ccactcctgt tacatctatt
      121 ccaaagaatt cctgaaatgt tcttaccgca agctcagtct gaggtccgaa aattccatcc
      181 gggtcagtcg gtgtaattcc ttccaggact gaagcaatat aagacaaata ttgttgcagt
      241 aagtaaacac cgggaccctc ggagcctacc cgatatgtga tacccggcca taacaaggcg
      301 ggaagggcaa tcgcggaagg gggaagtgtt tctaaaattc ctgatacagc attgaacatt
      361 gtattccagg tctgctcatc aacaagacct gttattggca agttaaatgt tttttggaat
      421 tccattaggg cagtcctcgt ttgggtattc aatacaccgc tgatatccac agaaggaata
      481 gagttgtagt atgcggacag aacattgaga aaatattgca caatttgaac tctcggaatt
      541 acctcttcag gaccgggagt aggagtgaat tcgggaattt cgctcaacat aattcctctt
      601 gaagtcaatt ccgctaattt tgtaactgca gcatatattc ttcttatacc gtaccatgtt
      661 gatttgtcaa taattcccgt caccgggaaa ttaaatatat cctggaactg tcttacagct
      721 tcttgcgttg cttcatcaaa ggttcctgta ggattaggta ttctttgtat agcaggatag
      781 tttgtggcta ttgtgtttaa acgtatctgc atcaataata catatggatt attgtctcct
      841 aattgaagag aaattccggg ataagtttca aaggcttcag ctatcggagt atctgttttt
      901 atatcaatat cttcaccata gtaatattga agcatttcat atggggtgta gccttgctct
      961 gccaattcaa ccgtacccca ttggagaagc cccggacacc aggagacgcg accgtcacaa
     1021 aattgagcat aaagaggctg aatttgtccc cgtcttatta tgtaactgtc aaaaagctca
     1081 tctgcaagca ccgatacagt gtcaaaaata cctctgttat gaacaaatgc ctggtcaaat
     1141 cttgtatcat ttgttatatc gaaattataa ccttgagctc ggtaccattc agtgaatata
     1201 cggtttaatg cggtagaagt aatggcatat atatttgctc taagtgcagc ttccggccaa
     1261 gtaggatata gttcgcttga ggcaacattt tttatataat caagaaacgg aaccgtaaca
     1321 ttttcacccg gttcatccgg tgcacctaaa tgaactgtaa ttgctgcagg tataaccact
     1381 tgtgtaagtc cgggaatttc tatactcata atatcacctc taatcatttg atatgtcaat
     1441 cggactggga ggaataacaa ttgttgtatc aggaccttct accggtccga ttgcaacagg
     1501 aatcatattt atttgaaaac gggtagtggt attcggaaaa attcttaaat caattacatt
     1561 taccgtataa tagtttattg cctgaactct taaattataa gtagtaaaat aatatctgcc
     1621 gctttcgaaa gggtcaaggc ggtcatacat tacgggtaat gtgataagcg gaattcgacc
     1681 gttggcatcg gttatatgat gcactatttc atgttctgca ccactttcat cacggatata
     1741 aaccgttact aaggcaccct ctaccggcaa tgctccggat gcggtaaaaa cacttaccga
     1801 taagtagccc acttcctgct caaaaggtat ggagcccggt ggaacttgtg ctttgtaaat
     1861 tcgaccatcg aatggattat atttcaaaat atttacacct ctctatctac ataacgaatt
     1921 atattattat atgcatccta aaatattttg tttatacagt tttggagcgg ctagattgta
     1981 gcgtcgggta attggattat gattttgctt gaaaattttc tgtaaaaatg ttacaattaa
     2041 ataaaagcat atcctaaaaa tgaggtggct taatattgct agccagagca ttcaaattaa
     2101 ttaaaaacct tttttcaaca tctaagacgg atgattgcaa aaaggaattt gctattgatt
     2161 tttgtaggat taatttagct aagttaataa tctctgcaaa aacacttatc gcattaggaa
     2221 ttattcagct cattgtatat tgtatagtca gtaagggaaa tctttttaca aagccacaga
     2281 tatatttcgg agcaatgaat tttattttgc tgtttgcaat gactatattt ttatcggtta
     2341 ttgttaagct ggaaaaggat gcagctaaaa atgtggtcag tattcacaat gctcaaatat
     2401 tctttataaa cttcatatta ttatggagcg gtgggatatc attgctgcat caactgacac
     2461 acggtcagac aatagtatat attgcagcgg ttataataac cgcaggcgct ttatatatag
     2521 aaccctctgt ttcatttctt atttatttta cttcacactt gattttttcc gtactattgc
     2581 ctaattttca aaagtcgccg gatttgttgt ttggaatata tttgaatact acttcattgg
     2641 taataatgtc atttgctatt tcctatacga gatataagag acaggagcag gattttaaaa
     2701 acagaaaaat catgcaggaa aacagcgatg aactgaagcg gattaataaa gaattagaag
     2761 aagcgaataa aaagctaaaa atcatatccc aatatgatgg attaacaggt attttcaatc
     2821 gacatacctt cgataccaaa attagagaag aatggaacag atgtaaaagg cattcaatgc
     2881 cattatcatt aataatggca gatatagacc attttaaatc atttaacgat aaattcgggc
     2941 accaggccgg tgataattgt ttaaaactgg ttgcagaaat tttatcggaa tgtgcaaagc
     3001 gctcttccga tgtagtggcc agatatggag gggaagaatt tgtgatattg cttcctcaaa
     3061 cagaaagaga aagagcatat gatttagctg aaaaaatgag aaaaagagta gaagaattaa
     3121 atataacaat cagcttaggt gttaatacta caatcccatc agatgaatca tccgttaggg
     3181 aattcataaa aaataccgat aaagctctat atatagctaa gaataaacgt aacagcacag
     3241 aagtatactt accaattgat actttcgggt tttcttaatg cttgatgctt taatataggc
     3301 accttgtatt tttctcccag ggcgcgtaca acatccttat agcatccttt taatttatca
     3361 tgaaaaacaa ttgcatcaat tccgccaatt atggcatcta cacattcaga tgctttttct
     3421 attccgtttt ctccttcgta ttcgcctgaa acaagccatt gatttgcaat tatgtgagaa
     3481 acaccgaaga attcattttc attccaacat gtgtggactg cctctgcaaa attcgggtca
     3541 aaatgtactc ctcccacgca aagaagatta tgcagcttta tatcatcaat atctttgtca
     3601 aatatatgaa gcagagaaag agcaagaacc ctgcaggcat ccatatttac ccagctttcc
     3661 ggtgtacttc ctatttctat gtctactatg ggtacgggat attttaagag aagctcaggg
     3721 tccccctgcc cttcataaac acccgaccaa tgggtcgctt cagttactac gctgaaatcc
     3781 aattcgattt cttttcttaa tttctccata gcccacataa gattgtgcat atataaagga
     3841 tttgcaggac cgtaacaacc tgaattaaca tcacctatac tatgtacggt aataacctta
     3901 tcaagagcat ttgtaccttc gtgccatgta accattccgg acatatcaaa atcagaaaaa
     3961 tacctgttca tatccggaag atacctggga taatccctgc ataatgctat atctgtagga
     4021 acaaaataga attcattatt tttattatcg atatatttat agacttctct tccgtcaaag
     4081 gtaaagtcaa ccttattgtt taagtatccc tcttccttta gtatatccca aaccaagtag
     4141 ctgacatatc cccaatcatg attgtcacag atataatata cggctttttt tacttttgtc
     4201 attatgccac tcctataatt attttaatta gtattatacc acctttttgc acaatgacaa
     4261 ggggacgtga caaggggacg ggggtattgt catctttttg
//
LOCUS       DAOSHP010000115         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_18610_length_4281_cov_10.906768, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000115 DAOSHP010000000
VERSION     DAOSHP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18610_length_4281_cov_10.906768"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..1257)
                     /locus_tag="PLB33_08655"
     CDS             complement(541..1257)
                     /locus_tag="PLB33_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63636.1"
                     /translation="MGYGGFPKYESAAENKEKANKSLAKLKKKNPDIEPIIIEGRTLA
                     KSWWGKSWNLNLESYADYTNRIDRGKSYVRNNMVLDLKITKGKVLAKVQGKRAKPYDV
                     EVSIDALSSEKWGHIISLCNNRIDSLEQLIEGKFPKELEVLFKEKKYGLFPSPKEIHF
                     DCSCPDWASMCKHVAATLYGIGTRLDSNPMLFFELRDIDSKELVRKSVEQKLESMLKN
                     AGKKSNREIAESDITEIFGL"
     gene            complement(1257..3902)
                     /locus_tag="PLB33_08660"
     CDS             complement(1257..3902)
                     /locus_tag="PLB33_08660"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOG63637.1"
                     /translation="MARMEIKEQELDAYFLANGFVLDEVSTLTEALIPWKEAFDNDKY
                     KALFHLGFLTKEKWFSPSMEYLHKISETLIKKLSQQPEIELVRDMVQAELTEDEIYQF
                     KDEIPFVIGMEYVDDNWLLNLWESILAVFKSEIKSYDGTVARYFADYNSNINVVGRVF
                     FHLVENKNEEYPFAFMATYTTKTVKSKRAIHTPLKNALSEFKGDEKKLISLISTVIKA
                     ADKSLFISGLLESGELFSPLRLKAEEAYTVLKEIAIYEEAGIMCRVPNWWRKKSNSIG
                     IEVKVGEKEPSKVGLSSIIDFTPTLKLGDEDITEKELIEFLKMAEGLIQYKGKWVEIN
                     KEKLEAALKAFERVKNFAFDENLTLSEAMKLELNMDKALDIPSDDIDVSISNGQWLRG
                     LKETMLHPEIMEKVSIVPSLNANLRKYQEKGYYWLNTMCRYGLGVCLADDMGLGKTVQ
                     MIAFLENKRVNNGGRALLILPASLIGNWQKEIEKFAPQMSYQILHKSDLKNSETIQIR
                     EGVFLYITTYGMALRLEELKEEKWDYLILDEAQAIKNPGTKQTKAIKALPAQMRIAMT
                     GTPIENRLSDLWSLFDFLNQGLLGTTKEFTNFTKELKENAVGYTKLRKMIQPFMLRRL
                     KTDKTIITDLPDKMEMNEYSDLTKKQIALYKQLIIQIEQKLNEAEGIERKGLVLSSIM
                     KFKQICNHPDQYLGREEYKAEQSGKFDKLREICETIYEKREKVIVFTQFKEMTEPLSD
                     FLKGIFTKEGFVLHGGTPVKKRNEMVSEFNSDKYIPYMVLSLKAGGVGLNLTAANHVI
                     HFDRWWNPAVENQATDRAFRIGQTKNVMVHKFVTKGTIEEKIDAIIEEKQKLSKDLLT
                     STGEQWITEYNNSEIMKIFALGGDL"
BASE COUNT         1275 a          893 c          515 g         1598 t
ORIGIN      
        1 aaaggatgct gtcaatatca agatgtcagt catgtaaatg ctcctttctt catatcatat
       61 aaaactttat cattcaaaaa atgtatatta tcttatcttt aatttacaat gtttatatta
      121 aactaaaaat aatattgcat taaaaaaagt aaaaataatg ctcctaattt ttattataat
      181 tataataccc ataccattac attttataac tgctttcaaa ttttttgagt tatgttcccc
      241 tgccttgttt taattgaata atgaataata aaaactaata acacaaacta aaatataatt
      301 aaatatgagc aaaataaagg atatttttta tgcaggcatc aactgcgacg ggcaattttt
      361 ccttgttcct caatacaatg aaaaaagata tcaaaacctt taaatcaatg gtttccgcct
      421 atccacatat ttaaccatgg atacaggtaa tgaaataatt tcagaaccgt aaaatttaat
      481 ttccgaatta tacggtattc ttatgccctt aaatcaagaa aagaaacggg aaaaggagtg
      541 ctacaatcca aatatttcag ttatatcact ttctgcaatt tccctgttgc tttttttgcc
      601 tgcattttta agcatacttt caagcttttg ctcaactgat tttctaacaa gttctttgct
      661 gtctatatcc ctaagctcga aaaaaagcat tggatttgaa tctaatctcg ttcctattcc
      721 atacaaggtt gctgctacat gcttacacat acttgcccaa tcaggacagc tgcaatcaaa
      781 gtgaatttct tttggagatg gaaataaacc atactttttc tctttaaata aaacttctaa
      841 ttctttaggg aatttacctt caatcaattg ttccaacgaa tcaatcctat tgttgcataa
      901 cgaaataata tgtccccatt tttcactgct taaagcatca attgaaactt caacatcgta
      961 tggctttgcc cttttaccct gtaccttagc caaaactttc ccttttgtta tctttaaatc
     1021 caaaaccatg ttgtttcgaa cataactttt tcctctgtct attctatttg tatagtctgc
     1081 ataactttca aggtttaaat tccaagactt cccccaccaa cttttagcca atgttcttcc
     1141 ttcaataatt attggttcaa tatccgggtt cttcttcttt aactttgcca aagacttatt
     1201 tgccttttcc ttattttctg ctgctgattc gtattttgga aatccgccgt aacccattat
     1261 aaatcaccgc ctaatgcaaa tatcttcata atttcactat tattgtattc tgttatccac
     1321 tgttcacctg ttgaagttaa taaatctttt gacagttttt gtttttcttc aataatggca
     1381 tcaatttttt cttcaattgt cccttttgtt acaaatttat gcaccattac attcttagtc
     1441 tgtcctatac gaaaagctct gtccgttgct tggttttcaa ctgccggatt ccaccatctg
     1501 tcaaaatgta tcacatgatt tgctgctgtc aggttaagac ctactccccc cgcctttaaa
     1561 gatagcacca tgtaaggtat atacttatcg ctgttaaact cgctaaccat ttcatttcgc
     1621 ttctttacag gagtgcctcc atgaagaaca aagccttctt ttgtaaatat gccctttaaa
     1681 aagtcagata atggctcagt catctcttta aattgcgtaa atactataac cttttctctt
     1741 ttctcatata ttgtttcaca tatttctctt aatttatcaa atttaccact ttgctcagct
     1801 ttatactctt ctcttcccaa atattggtcg ggatgattgc aaatttgttt aaacttcata
     1861 atgcttgata gaacaagccc cttacgttca attccttctg cttcatttaa cttttgctca
     1921 atctgaatta taagctgctt ataaagggca atttgtttct tagttaagtc tgagtattca
     1981 ttcatttcca tcttgtcagg caaatcagta ataatagttt tgtcggtttt caacctccta
     2041 agcataaaag gttgaatcat cttgcgaagt tttgtatatc caacagcgtt ttctttaagc
     2101 tcttttgtaa agttggtaaa ttctttggtc gttcccagta gcccctgatt aagaaaatca
     2161 aacaatgacc acaaatcgct taatctattt tcaataggcg ttccagtcat tgctattctc
     2221 atctgtgcag gtaatgcctt aattgccttt gtttgttttg ttccaggatt ctttatggct
     2281 tgtgcttcat ctaatattaa ataatcccac ttttcttctt ttaattcttc taatcttaaa
     2341 gccatgccat aggtagtaat atataagaat acaccttctc taatctgaat tgtttccgag
     2401 ttttttaaat cgcttttatg aagaatctga tatgacattt gaggagcaaa tttttcaatt
     2461 tctttttgcc agtttccaat tagcgatgca ggaagtataa gcaaagcccg cccgccatta
     2521 ttcactctct tgttttccaa aaatgcaatc atttgaacag tcttccctaa acccatatca
     2581 tccgccaaac aaacaccaag tccatatcgg cacattgtat taagccaata ataccctttt
     2641 tcctgatact ttcttaaatt tgcattaagt gatggcacaa tactaacctt ttccattatt
     2701 tccggatgaa gcattgtttc ttttagccct cttaaccact gtccattaga tattgatacg
     2761 tcaatgtcat cacttggaat atccaatgcc ttatccatat ttaattctaa cttcattgct
     2821 tcacttaaag tcaaattttc atcaaatgca aagttcttga ccctctcaaa tgccttcagt
     2881 gcagcctcta acttttcttt attgatttca acccatttac ccttatactg gattagaccc
     2941 tctgccattt ttaagaattc tattaattct ttttcggtta tatcttcatc tcctaatttt
     3001 aaagtaggtg taaagtctat gattgaggac aatcctactt tagatggctc cttctctcca
     3061 acctttactt caataccgat ggaattgctt tttttacgcc accagttagg aactctgcac
     3121 ataattcctg cttcttcata aattgcaatt tcttttaaaa ccgtatatgc ttcttctgct
     3181 ttcagtctta atggagaaaa caattcaccg ctttcaagca gtcctgaaat aaataaactt
     3241 ttatcagctg ccttaataac tgttgaaatc aatgaaatta atttcttttc atccccttta
     3301 aattctgata atgcattttt aagaggtgtg tgtatcgcac gcttactttt aactgtcttt
     3361 gttgtataag tagccataaa tgcaaaagga tattcttcat tcttattttc taccaaatga
     3421 aaaaacactc taccgactac attaatatta gaattataat ctgcaaaata tcttgcaact
     3481 gttccatcat aactttttat ttccgattta aatacagcca atattgattc ccacaaattt
     3541 aacagccaat tatcatcaac atattccatc cctataacaa acggaatttc atccttaaat
     3601 tgatagattt catcctcagt caattccgct tgcaccatat ccctaaccaa ttcaatttcc
     3661 ggttgctggc ttaacttttt tattagagtt tcagaaattt tatgcaaata ttccattgat
     3721 ggagaaaacc atttttcttt agttaaaaaa ccaaggtgaa ataaggcctt atatttatca
     3781 ttatcaaaag cttccttcca tggtattagt gcctctgtca gtgtagacac ttcgtctaat
     3841 acaaatccat tcgctaaaaa atatgcatct aattcttgtt cttttatttc cattcgtgcc
     3901 atctataatt ctcctctata tgtatagcgg tatattaatt acatcccctt gacatggatt
     3961 acacccattc taaggttaac taaaagtatt tcacggtcta tccccgtatt cccataaatt
     4021 tgccgcaaat attattatat tctctaaagc tgtaatattc aataataata tcatttcttt
     4081 ggcaattctt ctttatttgt taaagcacct gaacttttaa ttgaaatcat aagcccaagc
     4141 agccaatata cagacacatt cacaaagctt tataaatatt tcttgccgtt gcttgatgcg
     4201 tactggccta ttgtaaaatc tatatcccca ccatcattat tcattccagc cttttaacgt
     4261 atctaaatca aatgtccatt g
//
LOCUS       DAOSHP010000116         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_18897_length_4236_cov_8.684764, whole genome shotgun sequence.
ACCESSION   DAOSHP010000116 DAOSHP010000000
VERSION     DAOSHP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18897_length_4236_cov_8.684764"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..993
                     /locus_tag="PLB33_08665"
     CDS             46..993
                     /locus_tag="PLB33_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOG63638.1"
                     /translation="MKYLSKPFNNGIINLKNRLIMPPMATAKSETNGRITQDLIDYYD
                     EKSKGGYISLIIIEHSYISEDGKASKNQISIAEDSVIDDLRKLSDVIHKNGSKTVMQI
                     NHAGSRALEVGNSIHRGPLIDKDEIKEIVYNFRKAAIRVKEAGFDGVEIHSAHNYLLN
                     QFLSPITNKREDEYGGNIHGRIRIHLDVIKAVREAVGEEFPILIRLGATDDDVEGLTL
                     EDAVEAAKCLEKAGVDMIDVSGGMCRYMPSNSNEQGYFSTQSEEIKKAVNIPVMLTGG
                     ITDPYIADRMLENGKADFIGVGRSILYDSLWAKRAMELQ"
     gene            complement(1074..>2048)
                     /locus_tag="PLB33_08670"
     CDS             complement(1074..>2048)
                     /locus_tag="PLB33_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOG63639.1"
                     /translation="IQIIDIATPPMTHIDIAAKALKAGKHVICEKPLSGYFGQEGDVA
                     PIGKTVPKEKMFKKVMEEIDEFKKVVEESGKLFMYAENYIYCPSVTKAAEIIAHKKSK
                     LLFMKGEESLRGSSSPVAGRWDKTGGGSFIRVGTHPLSGILWLKQVEAKARGEKITVV
                     SVSADMGNTASMLNQYDRRHLTLETYDVEDFANVTLTFSDNTKAIVIGADNVLGGTKN
                     YIEIYANDTSLMCNITPANNLQSYFLDEDNLEDVSISEMLPGKTGWNNVFICDEILRG
                     YTFEMQDFVESVAYNRQPKSGIELAYETTKILYAAYQAAYEQKTVFLV"
     assembly_gap    2049..2148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2237..>4236
                     /locus_tag="PLB33_08675"
     CDS             2237..>4236
                     /locus_tag="PLB33_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOG63640.1"
                     /translation="MSSDLDIQNTIYDTIFEQVPFGIAVSYNCYPMKVSENKNASINA
                     MYEKITGRKREEMIKLGWAAITHPDDLQENLDKYNQLQEGKIDSYSMQKRFIRPDGSI
                     VWVDMTVARLNFEGSRYNHISIVQDITERKQMEDALIESERSKAVLLSNLPGMAYRCN
                     YDRDWTMQFVSEGCYELTGYKSGSLINNRDLSFNDLIAPEYQDILWNVWKKILIKKKP
                     FKYEYEIVSATGQRKWVLELGQGIYDEQGTVVALEGIIIDISERKEHENKLKYISEHY
                     ELTGLYNQRYFEELLVKESKLKRCDKKAIILINLKKINSVSLTYGYNFSRRLIVELAE
                     KLNQLNNNNIILFQISLDRFAFYIKKYKNEFELIDFCSKVFCKIKEVQILNNLGCKIG
                     IYEIEEDNWDLESIIKNVSVAADSVNNKSIFNYCFYNDELKAKVTRENDIRDELFKEI
                     TDDNSKTIYLQYQPILNLKTGKISGFEALARFKSQKLGLVQPSEFIPIAEEMQLIVPI
                     GLRVINKACNFIKKLETAGFDNINVSVNVSVIELLRDEFIDALKTLIKNAEINAKNLG
                     IEVTESIIMDNFEIINEKLYKLREMGIEVSIDDFGTGYSSLSRESELNVCNLKIDKYF
                     IDKINEQNTDKIIAGDIISMAHKLGHFVVAEGVENKEQYEYLL"
BASE COUNT         1473 a          614 c          805 g         1244 t
ORIGIN      
        1 ggcaccaaac tgaaaggcac agtgataaaa aaggagaaga tatctatgaa atatttatca
       61 aaaccattca ataacggaat aattaattta aaaaacagac tgataatgcc gccaatggct
      121 actgcaaagt cagaaaccaa tggcaggata acccaagact taattgatta ttatgatgaa
      181 aaatcaaaag gggggtacat atcactaata ataattgaac acagctatat ttcggaggat
      241 gggaaggcat ccaaaaacca gatttctatt gctgaagaca gtgtgataga tgatttaaga
      301 aaattatccg atgttataca taagaatggt tcaaaaacag tgatgcagat taatcatgca
      361 ggaagtcggg cattagaagt gggtaacagt attcatagag gaccattaat agacaaggat
      421 gaaattaaag aaattgttta taattttagg aaggctgcaa ttcgtgtcaa ggaagcaggc
      481 tttgacggtg tcgaaataca ttctgctcac aattatctct taaatcagtt tttatctcct
      541 ataacaaata agagagaaga tgagtatggc ggaaatatac atggcagaat aagaattcat
      601 ttagatgtta ttaaggcagt aagagaggct gtaggagaag aatttcccat tttgattcgc
      661 cttggtgcca cagacgacga tgttgaagga ttaacgctgg aggatgccgt cgaagcagca
      721 aaatgtctcg aaaaagccgg agtggatatg attgatgttt caggcggaat gtgcagatat
      781 atgcctagta acagcaatga gcaaggatat ttttcaactc agtctgaaga aattaaaaag
      841 gccgttaata ttcctgttat gttaacaggt ggaataacag acccatatat tgccgataga
      901 atgctcgaga atggaaaagc agattttatc ggtgtaggaa gatctatatt gtatgattct
      961 ttatgggcga aaagagcaat ggagctccaa taggggcgta aaagacgcca ggcaccaaac
     1021 gcaaccggtg cctggcacca ctttgttctt gtgtactcta tacaaggtgg tatctaaact
     1081 aaaaatacag ttttttgctc ataggctgcc tgataagctg cataaagtat ctttgttgtt
     1141 tcataagcca attcaattcc ggatttcggt tgcctgttat aagctacact ttctacaaaa
     1201 tcctgcattt caaaagtata tccacgaagt atctcatcgc agatgaatac attattccag
     1261 cctgtttttc ccggaagcat ctctgaaata cttacatctt ccaaattatc ctcgtctaag
     1321 aaataggact gcagattatt tgccggagtt atattacaca taagcgatgt atcatttgca
     1381 tagatttcaa tataattctt ggtgcctcct agaacattgt cagctccaat tacaattgcc
     1441 ttagtattgt ctgaaaaagt caaagttaca tttgcaaaat cctctacatc ataagtctca
     1501 agggtcaaat gtcttctatc atattgatta agcatagaag cagtattacc catgtcagct
     1561 gatacactga caactgtgat tttttcacct ctcgctttgg cttctacttg tttaagccac
     1621 aatattcccg aaagaggatg agttccgact ctaataaatg agccgccacc tgttttatcc
     1681 catcttccgg caacaggtga ggatgaaccc cttaaacttt cttcgccttt cataaacaat
     1741 aatttgcttt ttttatgggc aattatttct gcagcttttg taacagaagg acaatatatg
     1801 taattttcag catacataaa aagtttgccg ctttcttcaa ctactttctt aaattcatca
     1861 atttcttcca ttaccttctt aaacatcttt tctttaggaa cggtcttacc tattggagct
     1921 acatctcctt cttgtccaaa ataaccggat aatggttttt cgcaaataac atgtttgccg
     1981 gcttttaagg cttttgcagc tatatcaata tgggtcattg gaggagttgc aatgtcaata
     2041 atttgaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat gttttttgtt
     2161 gtaaaatttt acatataatg atataattat ataaataata ccaaattctg attataacag
     2221 acaatttgga ggaataatga gcagtgattt agacatacag aacacaatat atgatactat
     2281 ttttgaacaa gttccatttg gtattgccgt ttcatacaat tgctatccaa tgaaagttag
     2341 cgaaaacaaa aatgccagca ttaatgctat gtatgaaaag attacgggca gaaaaagaga
     2401 agaaatgata aagcttggtt gggccgcaat aacacatccg gatgacttgc aagaaaacct
     2461 tgataaatac aaccaattac aagagggtaa gattgacagc tattcaatgc aaaaacgttt
     2521 tataaggccg gacggttcaa ttgtatgggt tgacatgact gttgccaggc ttaattttga
     2581 aggatcaaga tataatcata taagtatagt tcaagatatt accgaaagaa agcaaatgga
     2641 agatgctttg atagagagtg agcgaagcaa ggcagtactt ctgtccaatt tgcccggtat
     2701 ggcttaccgc tgcaactatg atagggattg gacaatgcag tttgtttctg agggttgtta
     2761 tgaattgacg ggatataaat ctggcagctt aatcaataat agggacttgt cttttaatga
     2821 tttgattgct ccggaatacc aagatatatt gtggaatgtg tggaaaaaaa ttcttatcaa
     2881 gaaaaaacct tttaagtatg aatatgaaat tgtatctgcc accggacaaa gaaaatgggt
     2941 tttggaatta ggccagggta tttatgatga gcagggtacc gttgtagcac ttgaaggaat
     3001 tattattgat atttcagaaa gaaaagaaca cgaaaacaaa ctgaaatata taagtgagca
     3061 ttatgagcta acgggtttat ataatcaaag gtattttgaa gagctcttgg ttaaagaatc
     3121 aaaattaaaa agatgcgata aaaaagcaat tatattaata aatttaaaaa aaataaactc
     3181 ggtgagttta acttacggat ataatttcag cagaagactt atagtagaat tagcagaaaa
     3241 actgaatcag ttgaataaca acaatataat attgtttcaa atttctttgg acagatttgc
     3301 tttttatatt aaaaagtata aaaacgaatt tgaattaatc gatttttgca gtaaagtttt
     3361 ctgcaaaatt aaggaagtgc aaatattaaa taatttgggc tgcaaaatag gaatatatga
     3421 aattgaagag gataattggg atttggaaag tataataaaa aatgtttctg ttgcggctga
     3481 cagcgttaat aataaaagca tatttaatta ttgtttttac aatgatgaat taaaggctaa
     3541 agtaacgaga gaaaacgata taagggatga attatttaaa gaaataacgg atgataattc
     3601 taaaacaatt tatcttcagt atcaaccgat tttgaatcta aaaacaggga aaataagcgg
     3661 gtttgaagct ttagcccgct ttaaaagcca aaaacttggt cttgtacaac cttccgaatt
     3721 catacctata gctgaggaga tgcagcttat tgtaccaatc ggattaaggg ttataaataa
     3781 ggcatgcaat tttataaaaa agttggagac ggccggtttt gacaatataa atgtatctgt
     3841 aaatgtatct gtaatagaat tgctaagaga tgaattcatt gatgctttga aaacattgat
     3901 taaaaatgca gaaatcaatg cgaaaaattt aggtatagaa gtaaccgaat ctattattat
     3961 ggataatttc gaaattatta acgagaagct gtataaatta agggaaatgg ggatagaggt
     4021 ttcaattgat gatttcggaa caggatattc ttcattatcc cgtgaaagcg aactaaatgt
     4081 atgtaatttg aaaatcgata aatacttcat cgataaaata aatgagcaaa atactgataa
     4141 gattattgca ggtgatatta tatcaatggc tcataagctg ggacacttcg tggtggctga
     4201 aggtgttgaa aataaagagc agtatgaata tcttct
//
LOCUS       DAOSHP010000117         4162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_19347_length_4162_cov_8.371317, whole genome shotgun sequence.
ACCESSION   DAOSHP010000117 DAOSHP010000000
VERSION     DAOSHP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19347_length_4162_cov_8.371317"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLB33_08680"
     CDS             <1..410
                     /locus_tag="PLB33_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63641.1"
                     /translation="GRNFVGFDRCYTDFCYASFNEHNVIYRITERNDIMRKNFKFIFV
                     LSIIILIAYMYIAVDSAQRKSDEESYKILSDAIIRSAVQCYAIEGFYPPDIEYLENNY
                     GLLVDHNKYFVSYSVFASNIIPEVQVFLKNPNQ"
     gene            427..909
                     /locus_tag="PLB33_08685"
     CDS             427..909
                     /locus_tag="PLB33_08685"
                     /inference="COORDINATES: protein motif:HMM:NF027478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HOG63642.1"
                     /translation="MDRQNQFSFQFIFIMLLYLVIVILSVIVISLGKNIYDKINEDRN
                     KNYELQVSLSYIANKIRQSDINDAVEIKDLNGIDAVVINEVYDEEKYQTWIYFYDGAI
                     YEMFTDAGSTFELSDGSKVVEADLFKIEKVKDNLYKFTAVSKEENSELYLSLYSKAVT
                     "
     gene            909..1394
                     /locus_tag="PLB33_08690"
     CDS             909..1394
                     /locus_tag="PLB33_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63643.1"
                     /translation="MNKVSLNSKHSLLEIILSVLIFLAAGIIMLNCFGIARFTQIRAN
                     DKVTAGAIIQSDFEIIKSLNSTDELYEFLNTYGTKESGSINTYTKYYDENWIQGSGKE
                     YAVTIVIDDENTSSGTLININISAQKNNPYPFIKAGGQQIYSIESKKFFPSYGGAYEY
                     K"
     gene            1381..1851
                     /locus_tag="PLB33_08695"
     CDS             1381..1851
                     /locus_tag="PLB33_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63644.1"
                     /translation="MSISKTNVSVGMGGTLIITIFVVLCLMIFAVLSFVTAYSDLKLS
                     NKAQEITADYYYIHGKAEEKLAEISDVLYGIDINEEMDNILKKLSDIDGVYVDEENFK
                     LQYEVFGEENQKISVTLNLLHKDRFFYEIEAWNLSKIELPVYEDEIIDIWEGIE"
     gene            1853..2914
                     /locus_tag="PLB33_08700"
     CDS             1853..2914
                     /locus_tag="PLB33_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOG63645.1"
                     /translation="MDIINIFKTAIEKKASDIFIIAGRPLCYKILGDIVTFDEGILSS
                     EDTFKIISDIFNIANEKDLSVLEDKGDADFSFSLQKLGRFRVSAYKQRNSYAAVIRVV
                     FFELPDPKKLGIPDVVMDLYKKTKGLVLITGPAGSGKSTTLACIIDKINQERNCHIMT
                     FEDPIEFIHKHKKSIVSQREIYTDTNSYISSLRSALRQAPDVMLIGEMRDLETIEIAL
                     TAAETGHLVLSTLHTTGAANTIDRIIDVFPSNQQQQVRVQLSMQLQAVISQHLIPSVP
                     SGRVAAFEVMLTNNAVSNLIRESKVYQLNSIIHSSEYMGMKTMDASILELYKKGLITK
                     ENALIYATEPDSLLKQLNR"
     gene            complement(3030..3908)
                     /locus_tag="PLB33_08705"
     CDS             complement(3030..3908)
                     /locus_tag="PLB33_08705"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOG63646.1"
                     /translation="MRQMRKITIILMIVLFIASFTYCYGADNIYKGTKSEIDASNVED
                     GYVKIKYLKETTKKLKVIIEKDKGKYTYDLNNKGEYDVYPLQMGDGKYKIRVFENISG
                     NKYATKQTVTINVKLKEDKVPFLVPNQLVNFTNTSETVKKAAELTEGITDEFGKIDAI
                     YDYIITNIKYDTEKASTVKSGYLPTVDEILKTSKGICFDYASLMAAMLRSQGIPAKLV
                     TGYSSNLNVFHAWNEVYTEETGWIKLNEMYFDGFQWKLMDSTIASSGKQSNSPKVMEY
                     TNKLIDSKFYTKQFEY"
     regulatory      complement(4020..4106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1382 a          674 c          723 g         1383 t
ORIGIN      
        1 ctggtaggaa ttttgtcggt tttgacaggt gttatactga tttctgttat gcttccttta
       61 atgaacataa tgtcatctat aggataactg aaaggaatga tattatgaga aaaaacttta
      121 aatttatttt tgttctgtca ataatcattt tgatagccta tatgtacata gctgttgata
      181 gtgcccaaag aaaaagtgac gaggaaagtt acaaaatttt atctgatgca ataattagga
      241 gtgccgttca atgctatgct attgaaggat tttatcctcc cgatatagaa tatcttgaaa
      301 ataactacgg tttgttagta gaccataata aatattttgt aagctacagt gtttttgctt
      361 ccaacataat tcccgaagta caggtatttc taaaaaaccc gaatcaataa ttaggaagga
      421 aaaaatatgg acagacaaaa tcaattttca tttcaattta tattcattat gcttctttac
      481 cttgttattg taatcctgtc tgtaatagtt atttctttgg ggaaaaatat atatgataag
      541 ataaatgaag acagaaacaa aaattatgag ctgcaggttt ccctttcata catagcaaat
      601 aaaatcaggc agtctgatat aaatgatgct gttgaaatta aggatttgaa cggtatcgat
      661 gccgttgtga taaatgaagt atacgatgaa gaaaaatatc aaacttggat atatttttat
      721 gacggtgcga tttatgaaat gtttaccgat gcaggcagta cctttgaatt gtctgacggc
      781 tctaaggtag tggaagcgga cctctttaaa attgaaaaag tcaaggataa tttatataaa
      841 ttcactgcag taagcaagga agaaaattca gagctgtatt tatccctgta ctcaaaggcg
      901 gtgacttgat gaataaagta tccctgaatt caaagcattc acttttggaa ataattttat
      961 ccgttttaat atttttagcc gccggaataa taatgttaaa ttgcttcggc atagcaaggt
     1021 ttacacaaat aagagcaaac gataaggtaa cagccggtgc aataatacaa tcagattttg
     1081 aaattataaa atcattaaat agcaccgatg aattatatga atttctgaac acttacggca
     1141 caaaagaatc aggaagcatc aatacataca ccaaatatta cgatgaaaac tggattcaag
     1201 gcagcggtaa ggaatatgcc gtaaccatag taatagacga tgaaaatacg agctcaggca
     1261 cattgataaa tataaatatt tctgcccaaa aaaataatcc ctatcctttt attaaagcag
     1321 ggggacaaca aatttactct attgaaagca aaaagttttt cccttcctac ggaggtgcct
     1381 atgagtataa gtaagactaa tgtttctgta ggaatgggcg ggactctaat aataactata
     1441 tttgtagtgc tgtgcttaat gatttttgct gtattgtcat ttgtaactgc ttactcggat
     1501 ttgaaactgt ccaataaagc tcaggaaatt acggctgact attactatat tcacggcaag
     1561 gctgaagaaa agctggctga aataagcgat gtattgtatg gcattgacat aaatgaagaa
     1621 atggataata ttttaaaaaa attatccgat attgacgggg tttatgttga tgaagaaaat
     1681 tttaaacttc aatatgaagt atttggagag gagaatcaaa aaataagcgt aactttaaat
     1741 ttacttcata aagacagatt tttttatgaa atagaagcat ggaatttatc aaaaatcgag
     1801 ctgcctgttt atgaagacga aattatagat atatgggaag gaattgaata gtatggatat
     1861 tattaatatt ttcaaaactg ctatagagaa aaaagcttcg gatattttta taatcgcagg
     1921 cagacctcta tgctataaaa ttttgggtga cattgtgacc tttgatgaag gaatactttc
     1981 ttcggaagat acttttaaga ttattagtga cattttcaac atcgccaatg aaaaagattt
     2041 atctgtttta gaggataaag gagatgcgga tttttctttt tcactgcaaa agcttggaag
     2101 attcagggta agtgcatata agcagcgcaa ttcttatgct gccgtaatca gagtagtgtt
     2161 ttttgaactt cccgacccta agaaattagg cataccggat gttgtgatgg atttatacaa
     2221 aaaaactaag gggctggttc ttattaccgg tcctgccggc agcggaaaat ccacaaccct
     2281 ggcatgtata atcgacaaaa ttaaccagga gagaaactgt catataatga cctttgaaga
     2341 ccccatagaa tttattcaca agcataaaaa atcaattgta agccaaagag aaatttacac
     2401 agatacaaac agttatattt cttctcttcg gtctgcctta agacaagctc ctgatgtaat
     2461 gctgattgga gaaatgaggg atttggaaac tatcgaaata gctttaacgg ctgcggaaac
     2521 cggacatttg gttttatcaa cactgcatac cacgggtgct gcaaatacca tcgacagaat
     2581 aattgatgtt ttcccttcta atcaacagca gcaggttaga gtccagcttt caatgcagct
     2641 gcaggcggtt atttcacagc atctcattcc ttcagtgccg tcaggaaggg ttgcagcttt
     2701 tgaggttatg cttacaaaca atgccgtttc caatttaata agggaatcca aagtctatca
     2761 gcttaacagc ataattcatt cttctgaata tatgggtatg aaaacaatgg atgcaagtat
     2821 tttggaatta tataagaaag gtttgattac aaaagaaaat gcattgatat atgcaacaga
     2881 gccggattca ttacttaaac aacttaatcg gtagatagac acgcctctaa ctaaaaaatg
     2941 accctgtttg gtcatttttt agcgtgatga ggagattaat tcgaaattag gaaggaaaaa
     3001 cccgcccccc ttgtaatgcg ggataatcat taatattcaa attgtttcgt ataaaacttg
     3061 ctgtcaatta acttatttgt atattccata actttcggac tgtttgactg cttgcccgat
     3121 gatgcaatgg tactgtccat aagcttccat tgaaagccgt caaaatacat ttcgttcaat
     3181 tttatccaac ctgtttcttc cgtatacacc tcattccatg catgaaatac atttaagttt
     3241 gaagaataac cggttaccag tttagccggt ataccttgtg agcgaagcat tgcggccatt
     3301 aacgaagcat aatcaaagca tattcctttg ctggttttaa gtatttcatc tacggtaggc
     3361 agatatccgc tttttaccgt actggccttt tcagtgtcat attttatatt ggttattata
     3421 taatcataaa ttgcatctat ttttccaaat tcatctgtta ttccttcggt taattcggct
     3481 gctttcttta cagtttcaga tgtattcgta aaattaacca attgatttgg aactaaaaac
     3541 ggcactttgt cttcttttaa tttaacgttt atggttaccg tttgtttggt tgcatactta
     3601 ttaccgctta tattttcaaa tactcttatc ttgtatttcc cgtctcccat ctggagagga
     3661 tatacgtcat attcaccctt attgtttaaa tcgtatgtat attttccttt atctttttcg
     3721 ataataactt ttaatttttt tgttgtttct tttaaatatt ttattttaac ataaccatct
     3781 tcaacattag aagcatctat ttcggatttg gttcccttat atatgttatc tgctccataa
     3841 caatatgtaa atgaagcaat aaataacact atcatcagta taattgtaat ttttctcatt
     3901 tgtctcattg cttgtcttcc tttccttcaa tcttaactat agattgtcca cataatttat
     3961 aataaaaaag ccttgctgaa aacagcaaag ccaaccgtca tattaatgta ctcaataagt
     4021 acagcaactg gctgtcatac cattttcatg ggttagaccc tatagctttg cgtcattttc
     4081 tttagaaaat tttgccacat atttaattta aataataata acatgaatcc ttatacatgt
     4141 caacatattt tgacaatttt ta
//
LOCUS       DAOSHP010000118         4151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_19433_length_4151_cov_8.882568, whole genome shotgun sequence.
ACCESSION   DAOSHP010000118 DAOSHP010000000
VERSION     DAOSHP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19433_length_4151_cov_8.882568"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1099..2301
                     /locus_tag="PLB33_08710"
     CDS             1099..2301
                     /locus_tag="PLB33_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63647.1"
                     /translation="MKLFKMGSILIIVLASFTIIAQNSYSKKDIKEYSSFINQFIAVY
                     ADALVPLISTFKSDTVSENKITVALDQFGKKTNAYATVFADETKYVLMRDENLVELWP
                     KLNIDAKKKELLLERIGYLKEAMFNYTQKHDKQIFASEKVVNSIEQLRCFVDTIGSNY
                     EEIKAIEGLIQLNDSTNLGNSSSAHDNEQAEESATSEDDVQEYNARQIIDILNNDVFS
                     YYKDDLKDYDTPLKKQVFKKTDEYKELLSTIREIAKKIKNGKVKMIDEPGDSLSDYDL
                     KRNGFYYNVGANFTDYGAGNYPISKNGFCLDRIPTSKTKDIGGTIWRIFLPCDADTAV
                     NIEKNNDIIFVYTFKVTGVVDKVFTTFVMGTGYTVSYKAKYVSINNIELKIVNKTSNE
                     VLKTYFYK"
     gene            2438..2743
                     /locus_tag="PLB33_08715"
     CDS             2438..2743
                     /locus_tag="PLB33_08715"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOG63648.1"
                     /translation="MCSHICHQIPERSFNVLGVQFPICARCTGIYTGVIFGIIVKTKL
                     VRMKYLELFLLILSALALNTLSYINFFDVNILRYLFGFIFGLFCGQVIRKSVKEIVY"
     gene            2780..3142
                     /locus_tag="PLB33_08720"
     CDS             2780..3142
                     /locus_tag="PLB33_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63649.1"
                     /translation="MNFKNSRVVAFSSLICVIGLLFGVMIACGGSGSSNKEAEKISEV
                     SEVKKMSRDEFKKAVMYKNQKQVLEIFGKPDSTADMGKGVDWTYIRRTFDPINEKNDH
                     SVTVRFWYGRVEYLTFVQ"
     gene            3293..3829
                     /locus_tag="PLB33_08725"
     CDS             3293..3829
                     /locus_tag="PLB33_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63650.1"
                     /translation="MKNFLIMASTEIALVIAMLFLPSTTIADEYSSFKRTVNENGTET
                     FVIPVKSKEGQSILKLISTLESPGKPSPSETLKVFSPLKKDSWDLDLRYLRHVITGDD
                     PYYVYYIMFTWQSKDEDGIIHNIILAWSVFLDNNRNVKSWSPDASKWALNGELVSELS
                     EDDSWELAKLICSGEYFK"
BASE COUNT         1421 a          566 c          816 g         1348 t
ORIGIN      
        1 tctcttttgt aagcaagctt aaggagcatt tgatactagc cgttcccggc agcggtttcg
       61 gaaagaaagg atatttcaga cttacattct gtgtaggtga aaagacaata tcaaattctg
      121 cagaggcttt taagaaatca ataagtgcat gacgtcatgc atacgcaaaa ggtgcgatgc
      181 accttttgaa tctgtttttc ccgagagtca ggatcaacac aaggcttcgc cttatgaatc
      241 tttttttgca tggatcatgc aataatatca aagatgcccg cgaggcgggc agacacaaaa
      301 ggtgtttcac accttttaaa tccgtttgtc catttctttt gctgtgccca aaagaaatgg
      361 acgaaagaaa agggcaccgc tgcgcggggc ttgtcgggcg aattcctcgc accggctttg
      421 ccggatgctg cggtcgcccg agttgagtcc gacttcgtcg gactttttaa tggatattat
      481 ctctcattca tgttttgctg aagaattaag atgaatgaga taaagccctc tcccgtgatt
      541 gactatcttt attggctccg ccagtatatg tttcattttg gaagtgggca tgtagttctt
      601 agtgtatcaa ttgttttatt ttgagtttga caatataata atggcatcta caaagattat
      661 atgaatattg caattaaagc atttgcttta aagaggtgtt atattcagga aatactctga
      721 cacgcatcga gtaaaatggc tggaagatat gcgtatgtaa gcacgcatat aataaacaat
      781 atcctgagtc ggcttgatga tctatgagtt tcgtaaatta cgcctgatag tactgtttta
      841 tgcagggcat atgtcccgat aaatagggat cagttcaact tatgggatat aaggaaacgt
      901 ctgaaacggt ttagtatata tcagcaggtg ctatcattta tgcattggtt agagagcttg
      961 tgaaattact atggattcga ctccgaacct ttctcaataa aaaacaatca gcagaatttt
     1021 aatcattaat tatttattct tgacatacag tagatatatg tctaggttta tatgaaatta
     1081 aatatgccaa ggtgtaaaat gaaactgttt aaaatgggtt ctattttaat aattgtatta
     1141 gcttcattta caataatagc acagaatagc tactcaaaaa aagatatcaa agaatattct
     1201 tcgtttatta atcaatttat tgccgtttat gcagatgctc ttgttccttt aatctcaacc
     1261 ttcaaatctg atactgtttc agaaaataaa atcacggtag ctcttgatca attcggtaag
     1321 aagacaaatg catatgctac tgtatttgct gatgaaacaa aatatgtatt gatgcgtgat
     1381 gaaaatcttg ttgaattatg gcctaagttg aatattgatg ccaaaaaaaa agagctatta
     1441 cttgaaagga tcggatatct gaaagaagct atgtttaatt atacacaaaa acacgataaa
     1501 caaatatttg cttcagagaa agtagttaat agtatagaac aattgcgttg ttttgttgac
     1561 acaataggat ctaattatga agaaataaag gctattgaag gtttaattca gctgaatgat
     1621 tcgaccaatt tagggaattc atcttcagcc catgataatg aacaagcaga agaaagtgct
     1681 acatctgaag atgatgttca agaatataat gcacggcaaa ttattgatat attaaataat
     1741 gatgtttttt catattacaa agatgacttg aaagattatg atactccatt aaaaaaacag
     1801 gtgtttaaga aaactgatga gtacaaagaa cttttatcaa caattcgaga gatcgcaaaa
     1861 aaaataaaaa acggaaaagt gaagatgata gatgaacctg gagactcgct ttccgattat
     1921 gatttgaaaa gaaatggatt ctattataat gttggagcga attttacaga ttacggtgct
     1981 ggtaattacc caatttctaa aaatggtttt tgtcttgatc ggattcctac tagtaagact
     2041 aaagatattg gaggaactat ttggagaata tttttgccat gtgatgctga tacggcagta
     2101 aatattgaaa aaaacaatga tataattttt gtttatacat ttaaggtaac aggtgttgta
     2161 gacaaagtat ttacaacatt tgttatggga actggttata ccgtaagtta taaagctaaa
     2221 tatgtaagta taaataatat agaattaaaa attgtaaata aaacaagtaa tgaagtatta
     2281 aaaacctatt tctataagta atagaattta atttggcttt atatataaga atataataac
     2341 aagtattaga ttaattgtat taaataaaga ttcaaataat atttatgtaa aagacttgaa
     2401 attggaaata aatcatttat atattaaatt attaaatatg tgttcacata tatgccatca
     2461 aattcctgaa agatcattta atgttttagg cgttcaattt ccaatttgtg ctcgttgtac
     2521 tggtatttat actggtgtta ttttcggaat tattgttaaa acaaagcttg taaggatgaa
     2581 atatttagaa ttgtttttgt taatattatc agcacttgct ttaaataccc tttcttatat
     2641 taatttcttt gatgttaata ttttaaggta tctatttggg tttatatttg gattattttg
     2701 cggccaggtg ataaggaaat cagtaaagga aatagtttat taaattggtt tttaaaaaat
     2761 agtatatatg aggtagaata tgaattttaa aaattctcgt gtcgtggcat tttcatcttt
     2821 gatctgtgtt attggtttat tgtttggcgt catgattgcc tgtggtggga gtggaagctc
     2881 taacaaagaa gctgagaaaa ttagtgaagt atctgaagta aagaaaatgt ctagggatga
     2941 attcaaaaaa gctgtaatgt ataaaaatca aaaacaagta ctggaaatat ttgggaaacc
     3001 agattcgact gctgatatgg gaaaaggagt tgattggact tatattcgaa gaacatttga
     3061 ccctattaat gaaaaaaatg atcatagcgt tactgtacga ttttggtatg gtagagttga
     3121 atatcttact tttgtccaat aattactaat ttcactaaat tgacttctta atactttatt
     3181 aaaacataag aataaataca tggtggatta tatcaaataa tcattcaatc agagagtttt
     3241 gggaatgtag ttgctattaa attcaaataa aatagtagag gtgttttaat agatgaaaaa
     3301 ctttttaatt atggcatcaa ctgaaatagc attggtaatc gcaatgctat ttcttcccag
     3361 cacaaccatt gcagatgaat actcatcatt taaaaggact gttaatgaaa atggtactga
     3421 aacatttgta attcctgtta aatccaagga aggtcagagt attttaaaac ttataagcac
     3481 attggaatct ccgggtaagc caagtccatc ggaaacattg aaggttttta gcccgctcaa
     3541 aaaagatagc tgggatttag acttgcgata tctacggcat gtaataactg gtgatgatcc
     3601 ttattatgta tactatatca tgtttacatg gcaatccaaa gatgaagatg gaattattca
     3661 taatattata ttagcatggt ctgtattttt ggataataat cgaaatgtta agagttggtc
     3721 accggatgca tctaagtggg cattgaatgg agaactggtc agtgaattat ctgaagatga
     3781 ttcgtgggaa ttagctaaac ttatttgtag tggtgaatat tttaaataat aatttctaca
     3841 atgatggtat tcgccttaca tattttttgg aaatatatac gacaatcggc tccttcggga
     3901 gtcgattgtc gtataataga agcaaaagaa aagtgggacg gaaacagaga ggagaaaaaa
     3961 cagaatttgc cgaaggcaaa tttatctctg atcggagcgc aggggtcttg tctgagccga
     4021 atccgctgaa agcggttcgg cgagttctgc gcgacaaaga gcgtgagggg gttgcaaagg
     4081 ggcgagagtc ggcgactgag actttcgccc ctttgatctt ctgcatgaga ccatgcgaaa
     4141 cccggataaa g
//
LOCUS       DAOSHP010000119         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_20339_length_4022_cov_8.885304, whole genome shotgun sequence.
ACCESSION   DAOSHP010000119 DAOSHP010000000
VERSION     DAOSHP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20339_length_4022_cov_8.885304"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..783
                     /locus_tag="PLB33_08730"
     CDS             28..783
                     /locus_tag="PLB33_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOG63651.1"
                     /translation="MADFIKSNYIIEEKVKLKEIPAIIFRPRDAKEPIPTVVFYHGWS
                     SNKETQRMRGFILASVGYQVVIPDAIYHGERIPLADYSREAAIEYFWDVVFKNVEEYS
                     IIKNELVSKHAADSNRIAIMGNSMGGFTAGGIFTHNKDIRALIVLNGCCWWENFNRNA
                     DVTEKFERVEERVKELDPMNHLELLTDRPILLLHGDSDTSVPIESQNIFYNKLSYMYK
                     NKERIRLIEYHNLNHFVTTNMMEESINWLGRYL"
     gene            933..1529
                     /locus_tag="PLB33_08735"
     CDS             933..1529
                     /locus_tag="PLB33_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG63652.1"
                     /translation="MVTRREKEIEAMKELIISTAKEIIAREGFDNLSIRKIANKIEYS
                     PSIIYHYFKDKEEIVNIVMRRGYMKIVNAVYAADIESLTPRERLKRMTINYIEEALKM
                     PDEFLAAQINQSPQAHKHTSYLYKGASKEKPALGALYNCIKEIYRDADDEQIELKAQI
                     IAASTMGLIIKLVLEKNIGEEQRQNIINYFIEEVILKI"
     gene            1546..1932
                     /locus_tag="PLB33_08740"
     CDS             1546..1932
                     /locus_tag="PLB33_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG63653.1"
                     /translation="MKLVLKVLGIVFLLFVVVSVLSMFYLSRGLEEGLNVNIAPVDLI
                     LKEDGVYKGSYDFGRWSNELTITVKNNRITKIEITDDMMFVNPDVRNELFKRVMEKQN
                     TTVDAVSGGTVSCKAYLKSIENALNN"
     gene            1946..2428
                     /locus_tag="PLB33_08745"
     CDS             1946..2428
                     /locus_tag="PLB33_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOG63654.1"
                     /translation="MNTLIAYGTKYGCAEKCAIELSNELDGMVDTVNLKETNKIELAK
                     YDRVIIGGSAYIGKIQKEVTDFINLNLNELMKKEIGLFICGMQEGEELKKEIAENFPE
                     QLLEKAISVKHFGGEFIFRKMNFFERAIVKKISKVTSDKSDIKHDNIKKLAIELCNMN
                     "
     gene            complement(2580..3998)
                     /locus_tag="PLB33_08750"
     CDS             complement(2580..3998)
                     /locus_tag="PLB33_08750"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOG63655.1"
                     /translation="MKKELKKILVLLLVSMFVVASTTIGFAIPVTSENALRIHDDAIV
                     IDTHNDTMTRVIDRNTWLPVNDIGSLTSGQLDIPKMEAGGLDVAFFGSYTGGYTEIGR
                     AGSRLLALINALHWTERMNSEKFGLATSVNEIEKLVHEGKSVGVPTIEGAYSLEEYNA
                     IELLHQYYDLGIRVVGLCWSNSNSLGEGVNERYVDGTPSEGGLTELGAKVVKEMNKMG
                     MVVDVSHMNEETFWDVIEVAEAPIMASHSNVMGLYSHPRNLTDEQIIAIANTGGIVQQ
                     NFYAGYLGPTGDRDLKKLVDHIEYVIDLVGIDHVGLGSDFDGGGMPVDLPNASFYYRI
                     TEELVERGYKKSEIEQILGKNTLRVIKQVQNLAEKNPDNVGQGLTIIPELEMGEGVSG
                     NTPLLKAEVLRLNGSNIDESSFRVIVDGIVYTPEFNAETGELSLQVEEPLVEKFHVVT
                     FEAVNNSGKISRETRIFYIEEN"
BASE COUNT         1437 a          656 c          729 g         1200 t
ORIGIN      
        1 ggacaatata atatacaagg agaatttatg gctgatttta taaaatcaaa ttacataata
       61 gaggaaaaag ttaagctgaa agaaatcccg gcaattattt ttagaccgag agatgctaag
      121 gagccaatac ctacagtagt attctaccat ggctggagtt caaataaaga aactcagcgc
      181 atgagaggat ttatattagc ctcagtcggg tatcaggtag taattccgga tgccatttat
      241 cacggtgaaa ggataccgtt ggctgattac agccgggaag cagcgataga atatttctgg
      301 gacgttgtat ttaaaaatgt tgaagaatat agcattataa agaatgagct ggtgtcaaag
      361 catgctgcag actccaatag aattgcaata atggggaatt cgatgggcgg ctttactgcc
      421 ggcggaattt ttacacacaa caaggatata agagcactta tagtgttaaa cggctgctgt
      481 tggtgggaga atttcaacag gaatgcagat gttacagaga aatttgaaag agttgaagaa
      541 agggttaaag aattggaccc tatgaatcat ttagaactgt taacagacag acctatatta
      601 ctgctgcatg gtgatagtga cacttcagtg cccattgaaa gccaaaatat attttataat
      661 aaactaagtt acatgtacaa aaacaaagaa agaattagat taatagaata tcacaattta
      721 aatcattttg tcaccacaaa tatgatggaa gaaagcatta actggttggg cagatatttg
      781 tagaaattaa tattatgaat actgatgcta aggtatcagt atttttttat ttaaaaaaaa
      841 ataaaaaaaa gtattgacaa acagttatct aaatgatatt ttattaacga acggtgttca
      901 attaaagaac agtgttcagc gaaggaggta atatggttac tagaagagaa aaagaaattg
      961 aagcaatgaa ggaattaatc atatcgacag ccaaggaaat aattgcaagg gaaggctttg
     1021 acaatttgtc aattagaaag atagccaata aaattgaata ttcaccttca atcatatatc
     1081 actattttaa agataaagaa gaaattgtaa acattgtaat gagaagaggc tatatgaaaa
     1141 ttgttaatgc ggtttatgca gctgatatag aaagcttaac accaagggaa aggcttaaga
     1201 gaatgacaat aaactacata gaggaagcat taaaaatgcc tgatgaattt ctggcagcac
     1261 aaataaacca atcacctcaa gcacataagc atacttcgta tctttataaa ggagcttcaa
     1321 aagaaaagcc tgcattgggg gctctttata attgtataaa ggaaatctac agggatgccg
     1381 atgatgaaca aatcgaatta aaagcacaaa taattgcagc ttcaaccatg ggattgataa
     1441 taaaattagt gcttgaaaaa aatattgggg aagaacaaag gcaaaatata ataaattatt
     1501 ttattgaaga agtaatattg aaaatttaaa taaagggagt agaaaatgaa attagtattg
     1561 aaggtattag gaattgtgtt tttattattt gtagttgttt ctgtattaag tatgttctat
     1621 ttgagcagag gattggaaga gggattaaat gtaaatattg ctcctgtgga tttaatactt
     1681 aaagaggatg gcgtttataa aggaagctat gactttggca gatggtcaaa tgaattgaca
     1741 ataaccgtaa aaaacaacag aataacaaaa atagaaataa cagacgatat gatgtttgta
     1801 aatcctgatg taagaaacga gctttttaaa agagttatgg aaaaacagaa tactacagtc
     1861 gatgctgtat ccggtggaac tgtcagctgc aaagcatatc tgaaatctat cgaaaatgca
     1921 ttaaataatt aatcggagga aaataatgaa tacattaatt gcttatggaa caaaatacgg
     1981 ttgtgcggag aaatgcgcaa ttgaattatc aaatgaacta gatggcatgg ttgatactgt
     2041 aaacttaaaa gaaactaaca aaatagaact tgcaaaatat gaccgtgtaa taataggagg
     2101 gtctgcttat atcggtaaaa ttcaaaagga agtaacagac tttataaact taaatttaaa
     2161 tgagcttatg aaaaaggaaa taggattatt catttgcggc atgcaggaag gggaagagct
     2221 taaaaaggaa atagctgaaa actttcccga acagcttttg gaaaaagcaa tatcggttaa
     2281 acactttgga ggagagttta tcttcaggaa aatgaacttc ttcgaaaggg caatagttaa
     2341 gaaaatttct aaagttacat ctgacaaatc agacataaaa catgacaaca ttaaaaaact
     2401 ggcaatagaa ttatgtaata tgaattaata gggaaaatct taaaattgct ccggtaagat
     2461 tcaaatttgg agttgcacgg acacaaacgg gagaaaaaat ggaaatagac aaggctattt
     2521 ccattttata tacttcaatt cattcaatag ggagtaacct tttttttcaa aactatgagc
     2581 taattttctt caatatagaa aattctggtt tcgcgtgaaa ttttaccgct gttatttaca
     2641 gcttcaaaag tcacgacatg gaatttttct accaaaggtt cctcaacctg taaacttaat
     2701 tctccggttt cagcattaaa ttcaggtgtg taaactatac cgtcaactat tactctgaag
     2761 cttgactcat ctatattact tccattgagt cttagtactt cagcttttaa tagcggtgta
     2821 ttaccgctta caccttctcc catttcaagt tcagggataa ttgttaatcc ttgacctaca
     2881 ttatcagggt ttttttcagc taagttttga acttgcttta ttaccctcag agtatttttt
     2941 cccaatattt gttctatttc ggacttttta taaccacgtt ccacaagttc ttcggttatt
     3001 ctgtaataga acgatgcatt cggcagatct accggcattc ctcctccatc aaaatcggaa
     3061 cctaatccga catgatcgat gcctaccaaa tcaataacat attcaatatg gtcgactaat
     3121 tttttaaggt ccctgtcacc tgtaggtccc aaatatccgg catagaaatt ctgctgaaca
     3181 atgccgcctg tatttgctat tgcaattatt tgttcatcag taaggtttct cggatgagaa
     3241 tacaaaccca taacatttga gtgtgaagcc ataataggag cctctgcaac ttctatgaca
     3301 tcccaaaatg tttcttcatt catatgagat acgtcaacta ccatacccat tttattcatt
     3361 tctttaacta ctttagcacc gagttctgtt aatccgcctt ctgaaggggt gccatccaca
     3421 tacctctcat taacaccttc tccaagtgaa ttggaattgc tccaacataa tccaacgact
     3481 ctgattccaa ggtcataata ttgatgaagc aattcaatgg cattgtattc ttcaagagaa
     3541 taagctcctt ctatagttgg aacacctact gattttcctt catgcaccaa tttttctatt
     3601 tcatttaccg acgttgctaa cccgaatttc tctgaattca tacgctcagt ccagtgcagt
     3661 gcattaataa gtgccagcaa cctgctgcct gcacgtccaa tttccgtgta gcctccggta
     3721 tatgaaccaa agaaggccac atctagcccg cctgcttcca tttttggaat atctaattgt
     3781 ccggaagtta aacttccaat atcatttacc ggcaaccaag tatttctgtc aataactctc
     3841 gtcattgtat cattatgcgt atcaatcact atagcatcat catggattct caaggcgttt
     3901 tcagaagtaa caggaattgc gaatccaatt gttgtgcttg caacaacaaa cattgaaact
     3961 aacaacaaaa ctaaaatttt tttcaattct tttttcataa ttcgcctcct aataattttt
     4021 tt
//
LOCUS       DAOSHP010000120         3961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_20828_length_3961_cov_8.384537, whole genome shotgun sequence.
ACCESSION   DAOSHP010000120 DAOSHP010000000
VERSION     DAOSHP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20828_length_3961_cov_8.384537"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..379
                     /locus_tag="PLB33_08755"
     CDS             2..379
                     /locus_tag="PLB33_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOG63656.1"
                     /translation="MASIEEVQIILDEIAEELPPEIYKDLNGGIILLPQVKYHKKSVD
                     NDLYVMGEYRNEMISGRYIIVYYGSVERLYGNLTRNRLKNKLRSVVKHEFLHHLESLA
                     GEKHLEIEDERKLSEYLKKKDSF"
     gene            575..1873
                     /gene="lysA"
                     /locus_tag="PLB33_08760"
     CDS             575..1873
                     /gene="lysA"
                     /locus_tag="PLB33_08760"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOG63657.1"
                     /translation="MVREIKGNTLYFDGCNTVELANKYGTPLYVMSETAMVDKCKEIK
                     DTFLNKYERTRAAYASKAFLTLAMCKIIEREGLCLDVVSGGELYTAIKADFPAERIEF
                     NGNNKSIEELELAIDYNIGRIIVDGFDELGIIEDICKKKGKKTNILYRITPGVKSDSH
                     DYIVTGKKDSKFGFPLDDDVIFPAIEKAINSPYINFLGFHFHVGSQLHNNESHLKALK
                     ISLKLIKDTIDKYNYVTQELNVGGGFGIRYTDADDKKPYAYFLDPMMAEIEEFSKRMD
                     IKRPEIVIEPGRSIVGEAGITLYTIGTIKDIEGIRKYVSVDGGMTDNIRPALYQAKYE
                     CVIANKASQPSTDLVTISGKCCESGDILIRDAYIPLAERGDILAIFSTGAYGYAMASN
                     YNKNPFPATVLVKEGKSEVIVKRQTYEHMIENEVIPKSLV"
     gene            1893..2729
                     /gene="dapF"
                     /locus_tag="PLB33_08765"
     CDS             1893..2729
                     /gene="dapF"
                     /locus_tag="PLB33_08765"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOG63658.1"
                     /translation="MEFCKMQGAGNDFIIINNMKLNIPVDKLPAIAKKLCQRKVSIGA
                     DGLMAVDIPDGDADFKMLFFNRDGSIGEMCGNGARCISRYAYINNIAGKKMIFETGAG
                     IVSAEVLEGRLVKVMLNNPEVINLYNDIEIDGIKYELSYIELGNPGLPHAVVKYNLKQ
                     ADENKLREEGIKIRHYEGFPKGANVNFFEVLDENTALVKTYERGVEDFTLACGTGSAS
                     TALALILKGYLKNNQAKMINDGGELIIEVEKTDNKIEKLFLIGDTNIVAIGKIMDEDL
                     II"
     gene            2751..>3961
                     /locus_tag="PLB33_08770"
     CDS             2751..>3961
                     /locus_tag="PLB33_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOG63659.1"
                     /translation="MSTSKKTVWENYRFSVILIISIAIGSFIGLVFGDRAVILKPFGD
                     IFLNLMFTAVTPLVFVTIASAVGSMVNMRRLGRILGNMLLVFVITGLFAAVLIIFVVS
                     VYPPAEGVNIQIEAAGELEKLTFADQAVSAITVNDFSKILSRSNMLPLIVFSILFGYC
                     VSAVGGEDNIVARALDALSKVMMKFISVIMLYAPIGLGAYFASLIGEFGPQLLGAYAR
                     AMVVYYPLCVFYFFVFFALYTYYSGGMEAVKSYFKNIVEPSVTSIATQSSIATLPTEL
                     RAAQRIGVPKDIRDIILPIGATAHMDGTVLSSILKISFLFGIFGQEFAGIGTYLTAIG
                     LSILGGVVMSGVPGGGLIGEMLIVNMYGFPPEAFPIIATIGFLVDPPATWINSTGDTV
                     AAMMVTRIIEG"
BASE COUNT         1388 a          561 c          836 g         1176 t
ORIGIN      
        1 tatggcgtca atagaagaag tacaaattat attggatgaa atagcagagg agttacctcc
       61 tgaaatttac aaagacctta acggaggtat tattttgctg ccccaagtaa aatatcataa
      121 aaaaagcgtt gataatgatc tttatgtgat gggtgaatac cgcaacgaaa tgatttcagg
      181 aaggtatata attgtatact atggctctgt cgaaaggctt tatggcaatc ttacaagaaa
      241 caggcttaaa aataaactca ggtcagttgt caaacatgaa tttttgcatc atctagaatc
      301 attggccgga gaaaagcatt tagaaataga agatgaacgt aaattatcag agtatctgaa
      361 aaagaaggat tctttttaat tatatgaatt gaattccttc attaatacta ttgtaaacat
      421 agaaatacgg tttttatttt tgatttatag agccgttttt tttatttcaa taatttatct
      481 tgatttaaag gtaaaggctg cttataatat tgcataatta ttatataaag cgttataatt
      541 tttaatacta acacaaaatt tataaggaga taatatggtg cgagaaatta aaggtaatac
      601 attgtatttt gacggatgca atacggttga attggcaaac aagtatggga cgccgctgta
      661 tgtgatgagt gaaacggcaa tggtcgataa gtgcaaagaa ataaaagata catttttaaa
      721 caagtatgaa agaacaaggg ctgcatatgc ttcaaaagca tttttaactc ttgctatgtg
      781 caagataatt gaaagagaag gcctttgttt ggatgtggtt tcgggaggag aactgtacac
      841 agcaataaag gccgattttc ctgcagaaag aattgaattt aacggcaata acaaatcaat
      901 tgaagaatta gagcttgcaa tagattacaa tattggaaga ataattgtgg acggctttga
      961 tgaattggga attattgaag atatttgtaa gaaaaaaggg aaaaagacaa atattcttta
     1021 cagaataact ccgggtgtta aaagtgattc tcatgattat atagttacag ggaaaaagga
     1081 ttccaagttt ggatttccgt tggatgatga tgttatattt cctgcaattg aaaaagcaat
     1141 taattcaccc tacataaatt ttctgggttt tcattttcac gtaggctcac agcttcataa
     1201 caatgaatca catttaaaag cattaaaaat ttcattaaaa ttaataaaag acacaattga
     1261 taaatataat tatgtaacac aagaattaaa tgtcggcgga ggatttggaa taaggtatac
     1321 agatgctgat gataagaagc cctatgcata ttttttagac cccatgatgg cggaaattga
     1381 agagttttca aagagaatgg atatcaaaag acctgaaatt gttatcgagc cgggcagaag
     1441 tattgtagga gaggccggaa taacacttta tacaatcggc actataaagg atattgaagg
     1501 tataagaaaa tatgtgtccg ttgacggcgg aatgaccgac aatataagac ccgcattata
     1561 ccaggcaaag tatgaatgtg ttattgctaa caaagcatct cagccaagca ccgatttagt
     1621 aacaataagc ggaaaatgct gcgaatcggg ggatatattg ataagggatg catatatacc
     1681 attagctgaa agaggagata tacttgccat attttcaaca ggagcatacg gttatgctat
     1741 ggcaagcaac tacaataaaa acccttttcc tgcaacagtc ttggtcaagg aaggaaaatc
     1801 ggaagtaata gtaaaaagac agacatatga acatatgatt gaaaacgaag ttataccgaa
     1861 atcgctagta taaaataagg aagtgagcgc ttatggaatt ttgcaaaatg cagggagcag
     1921 ggaatgattt tataattatt aataatatga agttgaatat tcctgtagat aaattacctg
     1981 ccattgcaaa aaaactatgc caaagaaaag tatcaatcgg agcagacggg ttgatggcgg
     2041 ttgatatccc tgatggagat gcggatttta agatgctttt ttttaaccga gacggaagca
     2101 taggagaaat gtgcggcaat ggagcaagat gtatttctcg ctatgcatat ataaataata
     2161 tagcaggaaa aaaaatgatt tttgaaacag gagcaggaat tgtcagcgct gaagttttag
     2221 aagggcgttt ggtaaaagtt atgctaaata atccggaagt aattaatcta tataatgata
     2281 ttgaaataga cggaataaaa tatgaattat catatataga attaggcaat cccgggcttc
     2341 ctcatgcagt agttaagtat aaccttaagc aggcggatga aaacaaatta cgtgaagaag
     2401 gaatcaaaat aaggcattac gaaggctttc caaagggtgc caatgtaaac ttttttgagg
     2461 tgctggatga aaatacggca ttggtaaaaa cctatgaaag gggggtagaa gactttacat
     2521 tagcttgcgg caccggctct gcgtcaacag cccttgcctt aatactaaaa ggctatttga
     2581 aaaataatca agctaaaatg ataaatgatg gtggggaact gattatagaa gttgaaaaaa
     2641 cagacaataa aatagaaaaa ctgtttctta taggtgatac taacatagta gcaatcggaa
     2701 aaattatgga tgaagattta attatttaaa aattgttaaa ggagaataat atgagtacat
     2761 ctaaaaaaac agtatgggaa aactacaggt tttccgtaat attgattatt tcaatagcta
     2821 tcggtagttt tatcggtctt gtatttggtg atagagccgt aatattgaaa ccctttggag
     2881 atatattttt gaatctcatg tttacagcag ttactccatt agtatttgtt acaattgcaa
     2941 gtgctgtagg cagtatggtc aatatgaggc gtcttggcag gatattggga aacatgcttt
     3001 tagtatttgt aataaccggt ttatttgcag ctgttttaat tatctttgtt gttagtgtat
     3061 atcctcctgc agaaggagta aacatacaga tagaagccgc cggagaatta gaaaaattaa
     3121 cgtttgctga ccaagccgtt tctgcaatta cagtgaatga cttcagcaag attttatcac
     3181 gttccaatat gctgccctta attgtatttt ctattctttt cggctattgt gtaagtgcag
     3241 taggaggaga agacaatatt gtagcaaggg ctttagatgc attatctaaa gttatgatga
     3301 aatttataag tgtaattatg ctttacgcac cgataggact tggagcgtac tttgcgagtt
     3361 tgataggaga attcggaccg cagcttcttg gagcatatgc aagagccatg gtagtttatt
     3421 atcctctatg cgttttctat ttctttgtat tctttgcatt atatacatat tattcgggag
     3481 gaatggaagc ggtaaaatca tattttaaaa atattgtaga accatcggta acatcaattg
     3541 caacacaaag ctcaattgcg acacttccca cggaactaag agctgcccag cgtataggag
     3601 tgccaaaaga tatccgtgat atcatacttc ctataggcgc tactgcacat atggatggaa
     3661 cagtcttatc atcaatatta aaaatatcat tcttgttcgg catattcggt caggaatttg
     3721 cgggaatagg tacttacctc acagccatcg gtctgtctat attaggcgga gttgttatgt
     3781 ccggagttcc cggaggcgga ctgataggtg aaatgcttat agttaacatg tatggattcc
     3841 ctcctgaagc atttccaata atagcgacaa ttgggttctt ggttgacccg cctgccacat
     3901 ggataaactc tacaggagat actgttgctg caatgatggt aactcgtata attgaaggaa
     3961 a
//
LOCUS       DAOSHP010000121         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_21036_length_3933_cov_7.607530, whole genome shotgun sequence.
ACCESSION   DAOSHP010000121 DAOSHP010000000
VERSION     DAOSHP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21036_length_3933_cov_7.607530"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLB33_08775"
     CDS             complement(<1..1125)
                     /locus_tag="PLB33_08775"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG63660.1"
                     /translation="MENNSDDLYKRLAKYLDNLPGGFPETDSGVELRILKRLFTPEDA
                     ELALSLSLIAEEPRVIAHRANISVEEAAARLDEMEKKGLIYGKHYKDRPPKYTALQFI
                     VGIWEFQVNKLTPELVDDVNEYIPYWVNTDVWKRVPQIRSIPIDEAIDSELEVMSYES
                     AINLVQAQDRITVSPCICRTEQRTMGKGCDKPLETCLSFGDGADFYVRNGMGRSINKE
                     EALSILKKADAAGLVLSPGNSRKANFICACCGCCCGVLRTLKNFPHPADIVSSPFTAE
                     LNTENCIGCGICLERCQMEALKLDEKIILDKDRCIGCGLCVTTCPTNSLKLKRKIKDI
                     QHAVPRNFIEMNIKLGRARGKLSTGRLMKMIVKSKLDRMNA"
     gene            complement(1153..1494)
                     /locus_tag="PLB33_08780"
     CDS             complement(1153..1494)
                     /locus_tag="PLB33_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOG63661.1"
                     /translation="MPLTQKILDIALKYGEKNQSEKIITVCIRVGDLRDFVEEITQRY
                     WDYLSIGTIAYGAKIKFERVPVSAICNNCENIFYFDWHDEDKPCCTKCGNLDNRLITG
                     MELEVKEISIL"
     gene            complement(1532..2191)
                     /gene="hypB"
                     /locus_tag="PLB33_08785"
     CDS             complement(1532..2191)
                     /gene="hypB"
                     /locus_tag="PLB33_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOG63662.1"
                     /translation="MSDIKLINVKESILTDNDNDALFLREKLEKENVFMLNIMSSPGA
                     GKTSLIIATLKTLQDKYRIGVIEGDIDSMVDSEKIVQNGGHAVQINTGGDCHLDSFMI
                     KSAIDTMDLDNYDVIIIENIGNLVCPAEFDTGANLNVMILSVPEGDDKILKYPLMFQV
                     CDVLIINKIDCVKMFDFNFDALMNRAALVNPKMKIFQVSAKTGEGIEAWCDWLSEKIT
                     V"
     gene            complement(2252..3115)
                     /locus_tag="PLB33_08790"
     CDS             complement(2252..3115)
                     /locus_tag="PLB33_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOG63663.1"
                     /translation="MNENKIAIMTDSCSDVPRNLLERFHIYEMALSINYKDRSYRDRV
                     DITPEEVYENLQIEIPHTSLPTIGEIHETIEKIIADGYDQIIIPVISSGLSGTFKAIE
                     MVCEDFKNIKTKVIDTKNIAIGSGFLSIYAAQLVEKNFNFEEIVKKVESKIEDSNIYY
                     SLQTLHYLIKGGRLGRVEGMIGSILQIKPIISCDKDGIYYTVEKVRGRKQSINKLIEI
                     IQDEVKDKKNYYLAVCHGYAQEEADKIKERMQPYIDNATLFMEGQISPALGVHTGPGL
                     VGVGFLNLDED"
     gene            complement(3112..3672)
                     /locus_tag="PLB33_08795"
     CDS             complement(3112..3672)
                     /locus_tag="PLB33_08795"
                     /inference="COORDINATES: protein motif:HMM:NF020450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HOG63664.1"
                     /translation="MNNLELYEYSKTMSKVKLPVWDDLPDFPIYCDQLLQIVSDGISF
                     MQNGDEKLITKSMVNNYVKWGMMPKPVKKKYEKMHIAYVIVITILKQVLPISKIKDGI
                     HLQVALQGNEKTAYNSFCEAFEESMREVFVPILEKNSPYIMEEREIKYEKLAISSITT
                     ALSSKLLTEKIIETKIKNINNPGEQL"
BASE COUNT         1161 a          817 c          542 g         1413 t
ORIGIN      
        1 tgcattcatt ctatctaatt tggactttac tatcattttc attaacctgc cagtagaaag
       61 tttgccacgg gctcgcccaa gcttgatgtt catttctatg aagtttcttg gcacagcatg
      121 ttgaatatcc ttaattttac gcttcaattt aagcgaattg gttgggcatg ttgttacaca
      181 tagcccgcat cctatacaac ggtctttatc caaaatgatt ttttcatcca gcttaagtgc
      241 ttccatctga catctttcta aacatatgcc acagcctatg caattttctg tattgagctc
      301 tgctgtaaaa ggagaagaaa caatatcagc cggatgagga aagtttttca aagtcctaag
      361 aacaccacag cagcaaccgc agcaagcaca aatgaaatta gccttccggg agttacccgg
      421 cgaaagcacc aatcccgcag catcagcctt ctttagtatg gacaaggctt cttctttatt
      481 tattgaccgc cccattccgt ttcgcacata aaaatctgct ccatctccaa aagacaagca
      541 ggtttctaaa ggtttgtcac aacccttccc catggtgcgt tgttctgttc tgcaaataca
      601 tggcgataca gttattctgt cctgtgcttg aactaaattt atggcacttt cataagacat
      661 tacttccaat tctgaatcta tcgcttcatc tatgggaatt gacctgattt gtggcaccct
      721 tttccaaaca tccgtattaa cccaatatgg tatgtattcg ttgacatcat ccacaagctc
      781 cggtgtaagt ttattaacct ggaattccca aattcctact ataaattgaa gcgcagtata
      841 ttttggaggt ctgtctttat aatgtttgcc atatataaga ccttttttct ccatttcatc
      901 taaacgcgcg gcagcctcct ccacagaaat attcgcacga tgagcaatca cacgaggttc
      961 ttctgctatc aaagacaagg ataatgcaag ctcggcatct tcaggagtaa ataaacgctt
     1021 caatatacgc agttccactc cgctgtcagt ctcagggaat ccacctggta aattatctaa
     1081 gtatttcgcc aatcttttat ataaatcatc tgaattattt tccactactt cacttcctta
     1141 taacttcttt tattacagaa ttgaaatttc ttttacttca agctccattc cggttataag
     1201 ccggttatct aaatttccac attttgtaca gcatggctta tcttcatcat gccagtcaaa
     1261 ataaaaaatg ttttcacaat tattacaaat tgcagaaaca ggcacccgtt caaactttat
     1321 tttagctcca tatgcaatag ttccaatgct caaataatcc caatatcttt gcgttatttc
     1381 ttcaacaaaa tctcttaaat ctccaactct tatacacaca gttattattt tttcagattg
     1441 atttttttct ccatacttta atgcaatatc taaaattttt tgagttaacg gcaattcgtg
     1501 aataaggcac cactccttct aaacagcaaa cttagacagt aattttttca gaaagccagt
     1561 cacaccatgc ttctatgcct tctcctgttt tggcagaaac ttgaaatatc ttcattttag
     1621 gattgactaa tgctgcacgg ttcattaaag catcaaaatt aaaatcaaac atttttacgc
     1681 aatcaatttt atttattatc aatacatcgc aaacttgaaa cataagagga tatttgagta
     1741 tcttgtcatc gccttccggc acactgagta tcatgacatt tagattcgca cctgtatcaa
     1801 attcagctgg acaaacaaga tttcctatat tttcaataat tataacatca taattgtcca
     1861 ggtccatggt atctatcgca gattttatca taaaagaatc aagatggcag tcaccgcctg
     1921 tgtttatctg gacagcgtga ccaccatttt gaactatttt ttcagaatca accatggagt
     1981 ctatgtcgcc ttcaattacg ccaattctgt atttatcttg caaggtttta agcgttgcaa
     2041 taatcaagct tgttttgcct gccccaggtg aagacattat attaagcata aatacatttt
     2101 ctttttctaa cttctcacga agaaacaatg catcattatc attatctgtt aatatacttt
     2161 cttttacatt aataagcttg atatctgaca tcaatttcac ctcatcattt aatccgtaag
     2221 ttttatttgt tataattaat atttagagct tttaatcttc atctagattt agaaatccta
     2281 caccaacaag tcccggtcct gtatggacac caagagcagg ggaaatttgt ccttccataa
     2341 acaatgttgc attatctatg tatggctgca ttctttcttt gattttatct gcttcctctt
     2401 gagcataacc atggcatact gcaagataat aatttttctt atccttaact tcatcctgta
     2461 ttatttctat cagcttatta atgctttgtt tccttcctcg cactttttct actgtataat
     2521 aaatcccatc cttgtcacaa gaaataatag gtttgatttg aagaatactt ccaatcattc
     2581 cttctacgcg tcccaaacgt ccgcccttaa taagataatg taaggtttgt agactatagt
     2641 agatatttga atcttcaatt ttactttcta cctttttaac aatttcttca aaattgaaat
     2701 ttttttcaac taattgtgcc gcataaatgg acaaaaaccc ggatccaatt gcaatatttt
     2761 ttgtatctat aactttcgtt tttatattct taaaatcctc acaaaccatt tcaattgctt
     2821 tgaatgttcc gctcaatcct gaagatatta caggtattat tatttgatcg tatccgtcag
     2881 ctattatttt ctctattgtt tcatgaattt cgccaattgt tggcaaactt gtgtgaggta
     2941 tttcaatttg cagattttca taaacttctt ccggtgttat atcaaccctg tcacgataag
     3001 atctgtcttt ataattgatg ctgagtgcca tttcataaat atgaaatctt tccaataaat
     3061 ttcgaggtac atctgaacaa gaatcagtca ttattgcaat tttattttca ttcataattg
     3121 ttctcctgga ttatttatat tttttatttt tgtttcaatg attttttctg ttaacagttt
     3181 gcttgacaac gctgtagtaa tggaagaaat agcaagcttt tcatatttta tttctctttc
     3241 ctccattata taaggactgt ttttttctaa aattggaaca aagacttctc tcattgattc
     3301 ctcaaatgct tcacaaaagg aattataagc tgttttttca ttcccttgca atgcaacctg
     3361 caaatgaatt ccatctttaa tttttgaaat aggaagcacc tgttttagta tggtaatgac
     3421 aataacatat gcaatgtgca ttttttcata cttcttcttt accggtttcg gcatcatgcc
     3481 ccattttaca taattgttca ccatgctttt agtaattagc ttttcatcac cattttgcat
     3541 aaaagaaatt ccatcactga caatttgtag caactggtca caatagattg gaaaatcagg
     3601 taagtcatcc cacacaggaa gcttaacttt gctcattgtt ttgctgtatt catataattc
     3661 taaattattc atagttattt cctttattga taacgattat atgtggttat aataactact
     3721 taaataataa tccaaatata agtatctgtc aaatacaatt ttgtaattta aaatagaaac
     3781 aagggagaac agaatagctg ttcccccttg gaaaaattca ttgcctcatt gttcaacttt
     3841 taaggcttct gaatatcaat cctggcaaca aaactttgca gtatccccgc tttcatcaag
     3901 gctttttaca cacctactat ttcacccttg aca
//
LOCUS       DAOSHP010000122         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_21789_length_3843_cov_9.572598, whole genome shotgun sequence.
ACCESSION   DAOSHP010000122 DAOSHP010000000
VERSION     DAOSHP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21789_length_3843_cov_9.572598"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..833
                     /gene="nagB"
                     /locus_tag="PLB33_08800"
     CDS             111..833
                     /gene="nagB"
                     /locus_tag="PLB33_08800"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOG63665.1"
                     /translation="MEVIIKNNYEEISKLAADYLINTVKAKNDAILGLPTGSTPIGMY
                     QEVINRYKDNISFQNVRTFNLDEYVGLDKSNINSYRYFMDENLFSHIDIKEENIHIPN
                     GVARDIEKECINYENLLKTTGQMDIMYLGIGHNGHIGFNEPDEFFEPYTHIVKLTEDT
                     IESNKRFFDNIESVPQTAITMGIKTIMSAKKIILLASGESKAEAILKTVKGKITPRVP
                     ASILQLHNDVTLIIDKDAAKFL"
     gene            1043..2875
                     /gene="uvrC"
                     /locus_tag="PLB33_08805"
     CDS             1043..2875
                     /gene="uvrC"
                     /locus_tag="PLB33_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOG63666.1"
                     /translation="MFNIEEELKKLPDSPGVYQMKNAFGEIIYVGKAKNLKKRVRQYF
                     QSKNHIPKIQAMIKNIYEFEYIITDNEVEALILEANYIKKYRPKYNTLLRDDKQYPYI
                     KISVQERYPRIYKVREVKKDGAKYFGPYPSAYAVNEIIDIIGNLYKLRKCSLKLDGTK
                     KSQRPCVYYHINRCTAPCSGNVDIKEYSQEVNKATALLTGSEGIIKSLNEKMIEASEN
                     MEYEEAAKYRDQIKALEVISEKQKVVSQAGIDQDVIGSAIGIDEACIQIFFIRNGKII
                     GSEHYLLTNIENETREQIISSFLTQFYTGTVYIPKEIYIETKIEDMEVFEKLLSQKRG
                     NKVTLKVPVKGEKSMLIKMIKKNALEILEKGSRRIKKNIDEGMEAIKELSNVLNLDKT
                     PERIEAYDISNIQGVESVGSMVVFENGIPNKSNYRRFKIKTVEGPNDYKSLEEVLERR
                     INRGLGDGKQGFNKMPDLILIDGGKGQTNIAEEVISNFGLSIPVCGMIKDDKHTTKGL
                     IYKNKEIELRKTDIVYKLIYRIQEEAHRFAIEYHRNLRDKTLFKSELDNIKNIGEKRK
                     INLLKKFGSIENIKTKSINDLLSVPGMNTVAAESVYNYFHKEKN"
     gene            2917..3828
                     /gene="hprK"
                     /locus_tag="PLB33_08810"
     CDS             2917..3828
                     /gene="hprK"
                     /locus_tag="PLB33_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOG63667.1"
                     /translation="MQYVKLEDLVSYTGIEPVYYPEDVQSRIYTPDIVRPGLQMAGYF
                     EWFSFERVQVMGKAEILYIETLDEEVKKERLDKFFSFPIPALIVANEMEVDDLIIYYA
                     KKYKRPVFKSNERTDKLVNIMINFLEEELAEETTLHGVCLEVFGVGVLLRGKSSVGKS
                     ETALELINRGHRLVADDAVIIKKVEGGLKATCPELTRHLMEIRGIGILDIQHLFGVGS
                     IRIEQFVELVVDLEEWDENKEYDRIGKDEEYTEILGVKVSTVIIPVRPGRNTSAIIEI
                     AAKNYRQKLLGFNPLDTYNKKFRNMIR"
BASE COUNT         1518 a          480 c          755 g         1090 t
ORIGIN      
        1 gattgttttt tgccaaattt ttattgttta tttgcatttt ttgtgatacc atcaaaaaat
       61 aatttgggta tatcaaataa tcaatatata gaaaggaatg gattgaaaaa gtggaagtaa
      121 ttatcaaaaa taattatgag gaaataagta aattggcagc agattattta attaatacgg
      181 ttaaagccaa aaatgatgct atattaggac ttcctaccgg ttcaacaccg attggcatgt
      241 atcaagaagt aattaatcgg tacaaagaca acatttcatt tcaaaatgtg cgaacattta
      301 atttggatga atatgtagga ctagataaat caaacataaa cagctacaga tattttatgg
      361 atgaaaattt gttttctcat atagatataa aagaagagaa tattcatatt ccaaatggtg
      421 ttgcaaggga tattgagaag gaatgcataa attatgaaaa tttattaaaa acaactggac
      481 agatggatat aatgtatctt ggaataggac ataacggaca tatcggtttt aacgagccgg
      541 atgaattttt tgagccctat actcatatcg taaaacttac tgaagatact atagaatcta
      601 ataaaagatt tttcgataat attgaaagtg tgcctcagac tgcaataacc atgggtatta
      661 aaacaattat gtccgcaaag aaaataatac ttctggcttc gggagagtca aaggctgaag
      721 caatattaaa aacagtaaaa ggaaaaatca ctccccgggt tccggcttcc atattacagc
      781 ttcataatga cgtaacattg ataattgaca aggatgctgc aaaattcttg taagttatta
      841 tctttaacaa acaatattaa tctaagaatt tttaagtgca cctcaaatga ttagtcaatt
      901 gaaggtgcat tttatatata gacttaatgc gcagttaatt aaaaatagac gtgtaatatt
      961 cttaatataa aattaattta ttatttatgt taataataag gcaaatgtgg tataatatat
     1021 gtagtaatta tcaggagtaa aaatgtttaa tatagaggaa gaattaaaga aactgccaga
     1081 tagccccggt gtttaccaga tgaaaaatgc ttttggtgaa ataatatatg tgggcaaggc
     1141 taaaaattta aaaaagaggg tcagacaata ttttcagtct aaaaatcata tacctaaaat
     1201 tcaggctatg ataaaaaata tttatgagtt tgaatatatt attacggaca atgaagttga
     1261 agctctgatt ctggaagcaa attatataaa aaaatacaga cctaaataca atactctttt
     1321 aagagatgat aagcaatatc cgtatattaa aatatctgtt caagaaagat acccaagaat
     1381 ttataaggtc agagaagtaa aaaaagatgg ggcaaaatat ttcggaccct atccgagtgc
     1441 ttatgccgta aatgaaataa tagatattat aggaaactta tacaagctta gaaaatgctc
     1501 tttaaaactt gacggtacaa agaaaagcca aagaccatgc gtatattatc atataaacag
     1561 gtgtaccgca ccctgtagtg gaaacgttga cataaaagaa tacagccaag aagtaaataa
     1621 agcaacagct ctattgacag gcagtgaagg cattattaaa tcgttaaacg aaaaaatgat
     1681 tgaagcttca gaaaatatgg aatacgaaga agctgccaaa tatagagacc agattaaggc
     1741 tcttgaagta atttcagaaa aacaaaaagt tgtttctcaa gcaggaatag accaagatgt
     1801 tataggaagc gcaattggta ttgatgaggc ctgcatacag attttcttca ttcgaaacgg
     1861 taaaattata ggcagcgagc attatttgct tacgaatata gaaaatgaaa caagggaaca
     1921 aattatcagc tcatttttaa cacaattcta tacaggtaca gtatatattc caaaagagat
     1981 atatatagaa accaaaatag aagatatgga ggtttttgaa aaactcttgt cccaaaaaag
     2041 aggaaacaag gttactttaa aagttccggt taaaggcgaa aagagcatgc ttattaaaat
     2101 gataaagaaa aatgctcttg aaatattaga aaagggaagc aggagaataa agaagaatat
     2161 tgatgaaggc atggaggcaa tcaaagagct gagtaatgtt ttaaatttgg ataaaactcc
     2221 cgaaaggatt gaagcttatg atatttcgaa tatacaagga gttgaatctg taggctccat
     2281 ggttgttttt gaaaacggaa ttcccaataa aagcaattac cgtcgtttca aaataaagac
     2341 agtagaggga ccaaatgact ataaaagttt ggaagaagta ttggaaagaa gaattaacag
     2401 agggttagga gatggcaagc aaggtttcaa caaaatgccc gacttaattt taattgacgg
     2461 cggaaaggga caaacaaata ttgcagaaga agtaataagt aattttggtt taagtattcc
     2521 tgtttgcggt atgattaagg atgacaaaca tacaactaaa ggattaatat ataaaaacaa
     2581 ggaaattgaa ctcagaaaga ccgatatagt ttataaatta atttacagga ttcaggaaga
     2641 agctcataga tttgcaattg agtaccacag gaatttaagg gacaagacac tgtttaaatc
     2701 agagcttgat aatattaaaa atatcggaga aaaaagaaaa attaatttac tcaaaaaatt
     2761 cggctcaatt gaaaatatta aaactaaaag tattaatgat cttttgtcgg tacccggaat
     2821 gaatactgta gcggcagaat cggtttacaa ttattttcac aaggaaaaaa actagtcatt
     2881 agattatgat tacttaatat agagggatgg tacaatatgc agtatgtaaa acttgaagat
     2941 ttagtcagtt acacgggaat agaaccggtt tattatcctg aggatgttca atccagaata
     3001 tatacacctg atatagtaag gccgggtctt caaatggcag gatattttga atggttttcc
     3061 tttgagagag ttcaagtaat gggaaaagca gaaattttat atatcgaaac attggatgaa
     3121 gaagtaaaaa aagaaagact ggacaaattt ttcagctttc ctataccggc attaattgtg
     3181 gcaaatgaaa tggaagttga tgatcttatt atttattatg cgaaaaaata taaaagacct
     3241 gtttttaaat ctaatgagag aacagataaa ttagttaata taatgattaa cttccttgaa
     3301 gaagaattgg ctgaagaaac aactttgcac ggtgtttgct tggaagtatt cggggttggg
     3361 gtattattaa gggggaaaag cagtgtcggc aaaagcgaaa cagccttaga gctgataaac
     3421 agggggcaca gacttgtggc tgatgatgcg gtaataataa aaaaggttga gggagggctt
     3481 aaagcaacct gccctgaatt aaccagacac ttaatggaaa taaggggtat aggaatactt
     3541 gatattcagc atttgtttgg cgtaggctcc atcaggattg agcagtttgt agaattggtg
     3601 gttgatttgg aagagtggga tgaaaacaag gaatatgaca gaatcggcaa ggatgaagag
     3661 tatacggaaa tattaggtgt taaggtatct acggttatta taccggtcag accgggaaga
     3721 aatacatcag ctattattga aatagctgcc aaaaattaca gacagaaact tcttggtttt
     3781 aatcctcttg atacttacaa taaaaaattt cgaaatatga ttcggtaaaa atgcaacttt
     3841 att
//
LOCUS       DAOSHP010000123         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_22086_length_3809_cov_9.775173, whole genome shotgun sequence.
ACCESSION   DAOSHP010000123 DAOSHP010000000
VERSION     DAOSHP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22086_length_3809_cov_9.775173"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLB33_08815"
     CDS             complement(<1..640)
                     /locus_tag="PLB33_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOG63668.1"
                     /translation="MTDNLNIFNAMKIKLSDELPEYPVLKENVRRAPKREVTLNEREI
                     KLALKNALRYVPGSLHEKLAPEFLDELMTYGRIYGYRFMPKERIYGKPIDEYKGKCIE
                     GKAFQVMIDNNLDHEVALYPYELVTYGETGQVCQNWMQYQLIKKYLEALTEEQTLALM
                     SGHPLGLFKSHKNSPRVIISNGLMVGMFDNPQDWAKATAMGCSSYGQMTAGGW"
     gene            complement(780..1691)
                     /locus_tag="PLB33_08820"
     CDS             complement(780..1691)
                     /locus_tag="PLB33_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOG63669.1"
                     /translation="MLNFKKIELEDKEWIIPILKESVITGCHQNFGNLFAWRDINNTR
                     VANINNFLVAKQLVNGKDVYLYPMGKGDVKPVIEEIIRSADKNLIFAGLSHDEMIELN
                     ELYPGKFIYDQVRNDFDYIYLLEKMVSLKGKKLHRKRNHISAFLRNNPVWSFETITEE
                     NKAECIEMNKKWCIESGCKDDESLMDENCATKYFFKYYDELELEGGLIRVEDKIVAYT
                     IGEVLSNDTYVLHIEKAFKDVQGAYPMINQRFASWIKDKYPHLIYINREEDMGSEGLR
                     KAKLSYYPDKMAIKYRAVTKKNYALHK"
     gene            complement(1797..2375)
                     /locus_tag="PLB33_08825"
     CDS             complement(1797..2375)
                     /locus_tag="PLB33_08825"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOG63670.1"
                     /translation="MNLEFAEYSEIFIKYLETWGPAAAIILPVFEAFLPILPLVGFVI
                     INVAAFGFFFGYLYSWIGNCLGSFLLFLLLRKIGGKRINEKIKSSKFRYTLEKVKKKQ
                     LNVLFLLYCFPFAPSFLLSGTAALTNMRTEMFLAILLPSKFVMLLSLAFIGENVSSFF
                     ENPAKSIFYIIFIVLLNLLVKYLFEKYEQRKQ"
     gene            2622..3122
                     /locus_tag="PLB33_08830"
     CDS             2622..3122
                     /locus_tag="PLB33_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOG63671.1"
                     /translation="MKYFIGAPIPDNYKNKIEMLRAEFRFFTTEPHITLVPPPALPDE
                     DDFIEEVVEVCKKVKPFDIRLNNLDQFGSRVLYVSVDSPGLIDLHNKIYEKLCLQHER
                     RGFTPHLTIVKQRPKRPVDIATIRKRAEIKLIPSPEYTLNSITVYHQPKEHSIYIPYM
                     EIPLGE"
     gene            complement(3156..>3809)
                     /locus_tag="PLB33_08835"
     CDS             complement(3156..>3809)
                     /locus_tag="PLB33_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOG63672.1"
                     /translation="SNFEESSGKYKYKSDFSMFIEESREEDFYTGTDKITVSTIHKSK
                     GKEFDHVIMVLRDLNLNREETKRLIYVGMTRAKKSLSIHFNGKFIERKIGFIDMILRN
                     IKYEFDNEEYPPAEIISMQLKYNDVYLSYFYKTIKLVQKLNSGDILHFDENGCKDENG
                     VYILLFSSKFKNEIQTYIEKGYKPFSAKVNHILFWKEENKEKETLIVLPQLTFIKSN"
BASE COUNT         1227 a          715 c          519 g         1348 t
ORIGIN      
        1 tccagccgcc tgccgtcatt tgcccatagc ttgaacatcc cattgcggtt gccttcgccc
       61 aatcctgcgg attgtcaaac attcctacca taagtccgtt tgatattata actctggggc
      121 tgtttttatg ggatttaaac aatcccaacg gatgaccgga cataagggct aatgtttgct
      181 cttccgttaa tgcttcaaga tattttttta ttaattgata ctgcatccaa ttttggcaaa
      241 cctgacctgt ttccccgtag gtaaccaatt catacgggta aagggcaact tcatggtcta
      301 aattattgtc aatcataact tggaatgctt tgccttctat acatttcccc ttgtactcat
      361 ctataggctt accgtaaatt ctttctttag gcatgaaacg gtagccgtaa atccttccat
      421 atgtcatcaa ttcatccaaa aattcgggag ctaatttttc atggagactt cccggcacat
      481 accttaatgc attcttcaat gccagcttta tttctctttc atttaaagta acttctcttt
      541 tgggagcacg tcttacattt tcctttaaaa ccggatactc tggtaattca tcgcttaact
      601 tgattttcat tgcattaaaa atatttaaat tatcagtcat attacacctc ctaaaacaaa
      661 ttaaaaaaaa ttttatatct taagattact taaagctttt tcgggtaaac tatatataag
      721 ttaaatagta aatttaactt ccctctcccc cttgtttttt tataaacggt tattatgcct
      781 tacttatgta aggcataatt ttttttagtc acagcgcgat atttaatcgc catcttgtca
      841 ggataatatg aaagttttgc tttccttaag ccttcagagc ccatgtcttc ttctctgttt
      901 atgtaaatca aatgaggata tttatcttta atccaggaag caaatcgttg gtttatcatc
      961 ggatatgccc cttgaacatc tttaaacgct ttttcgatat gtaatacata tgtatcatta
     1021 cttagaactt cacctatggt ataagccaca attttatctt ctaccctgat taaaccgcct
     1081 tcaagctcga gctcgtcata atatttaaag aaatatttag tggcacagtt ttcatccata
     1141 agactttcat cgtctttaca tcctgattca atgcaccatt ttttgttcat ttcgatgcat
     1201 tcagctttat tttcctcagt tattgtttca aatgaccaaa caggattatt tcttagaaag
     1261 gcactgatat ggtttctttt tctgtgaagc tttttccctt ttaatgaaac cattttttcc
     1321 aacagataaa tatagtcaaa gtcatttctt acttgatcat aaataaattt ccccggatac
     1381 aattcattca gttcaatcat ttcatcatga gataatcctg caaagattag atttttatct
     1441 gcagacctta ttatttcttc aattacgggt ttaacatctc cttttcccat tggataaaga
     1501 tatacatctt ttccattaac taattgtttg gctaccaaaa aattattgat attagcaact
     1561 ctagtgttat ttatgtccct ccatgcaaac aaatttccga aattctgatg acatcctgta
     1621 ataaccgatt ccttcaatat gggaataatc cattctttat cttccagttc aatcttttta
     1681 aagttcagca ttaataattt tctccttgtc acgttgtcca atcgtatagt attttttcct
     1741 cattagaaat atattacatt acacattata cactatatca taaaaaaaat caccaattac
     1801 tgttttcgtt gttcgtattt ttcgaacaaa tacttcacta atagatttaa cagcacaata
     1861 aaaataatat agaatattga ctttgcagga ttttcaaaaa atgagcttac attttcaccg
     1921 ataaaagcca atgaaagaag catcacaaat tttgacggca gtaatatagc aaggaacatt
     1981 tccgtcctca tattagtcaa agctgcagtg ccgctcagta aaaatgacgg agcaaaagga
     2041 aagcagtata ataaaaacag cacgtttaat tgttttttct ttaccttttc aagggtgtag
     2101 cgaaatttgc tactttttat tttttcattt attctttttc ctccgatttt ccttaacaat
     2161 aagaataaaa gaaacgatcc taagcaatta ccaatccaag aatataagta tccgaaaaaa
     2221 aatccaaaag cagctacatt tattattaca aatcctacca atggcagtat gggtaaaaat
     2281 gcttcaaaca caggaagaat tatggctgca gcaggtcccc acgtttctaa atattttatg
     2341 aaaatttcag aatattccgc aaattccaaa ttcattatca aacctccacc ggtcagacta
     2401 tctatattta ttatacccat cattagattt acatacaaaa caaacaaaac atttatataa
     2461 attaagtgaa acactattaa tattaacata aatgcttgat aattttaaat aaaaaatata
     2521 aaattatctc atgagattct tcacagtgtt cagaatgaca atatgtgtat tcatggcaag
     2581 acagggtaaa taattataaa aaagatatag aaaggattta tatgaaatat ttcataggag
     2641 cccccatacc tgataactat aaaaataaaa ttgaaatgct aagagctgaa tttagatttt
     2701 tcacaaccga acctcacatt actttggttc ctcctcctgc acttcctgat gaggatgact
     2761 ttattgagga agtagttgaa gtatgcaaaa aagttaagcc ttttgatata aggttgaata
     2821 atcttgacca gtttggcagc agagttttat atgtaagtgt tgattctccc ggtttaatcg
     2881 atttacataa taaaatatat gaaaaattat gcttgcagca tgagagaagg ggatttaccc
     2941 cacatttaac aattgtaaag caaagaccca aaagacctgt agatatagcc acaataagaa
     3001 aaagggcaga aataaaactt attccttccc cagaatatac tttgaattca attaccgtct
     3061 accatcaacc aaaggagcat tccatttata ttccctacat ggaaattcct ttgggagaat
     3121 aacacagact gttcttctct gaaattcctc tgtaatcagt ttgatttgat aaatgttagt
     3181 tgagggagaa ctatcaatgt ttccttttcc ttgttctctt ccttccagaa taatatgtga
     3241 ttaactttag cagaaaaagg tttgtagcct ttctctatat aagtttgaat ttcgttttta
     3301 aactttgatg aaaaaagtaa tatatataca ccattttcgt ccttacagcc gttttcgtca
     3361 aaatgtagta tatcaccact attcagcttt tgaactaatt ttatggtttt gtaaaaatac
     3421 gacagataca catcgttata ctttaattgc atggaaataa tttctgcagg cggatattct
     3481 tcattatcga attcatattt aatgtttctc aaaatcatat caatgaatcc gatttttctt
     3541 tcaataaatt taccgttgaa atggatggat aaggactttt tagctcttgt cattccaaca
     3601 tatatcagcc tttttgtttc ttcccggttt aaattcaagt cccttaatac cataatgaca
     3661 tggtcaaact cttttccttt ggatttatga atagtggaga ccgttatttt atcagtgccg
     3721 gtataaaaat cttcttctct agattcttca ataaacatgg aaaaatcaga cttatattta
     3781 tattttccgg aactttcttc gaaattgga
//
LOCUS       DAOSHP010000124         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_22814_length_3725_cov_9.267030, whole genome shotgun sequence.
ACCESSION   DAOSHP010000124 DAOSHP010000000
VERSION     DAOSHP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22814_length_3725_cov_9.267030"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(219..302)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            602..1003
                     /locus_tag="PLB33_08840"
     CDS             602..1003
                     /locus_tag="PLB33_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOG63673.1"
                     /translation="MGKKIRVKPSKAQSTAAFFVGIVFCLIGLFVAIPMAGPFGIFWT
                     LVAVIITVMHGKNAFTEEGVSTHEIIIEDNDFIKKTKLINNTAEETENEDYVNISDSK
                     KRLEEIKLLYENGLINKDEYEEKRKSIIDNI"
     gene            complement(1066..2451)
                     /locus_tag="PLB33_08845"
     CDS             complement(1066..2451)
                     /locus_tag="PLB33_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOG63674.1"
                     /translation="MDVKYVAEPFRIKMVEPLKMLSREEREQKIKEANYNVFNLKAED
                     VYIDLLTDSGTNAMSSYQWAGVMLGDESYAGGTSYYKLIEAGQDIFGYKIIQPVHQGR
                     AAEKVFFPILLSKGKYAIANMFFDTTRAHVEIAGARAIDCVVEEAKDPTLRAPFKGNM
                     DVEKLEKLINELGRENVGVVVMTITNNSAGGQPVSVQNMRDVSAVCKKYNIPLDIDAA
                     RFAENAYFVKQREEEFKDWSIKDITREMFSYADFFTMSAKKDAIINMGGMIGIKEDNQ
                     EFYQKVKANCISFEGFTTYGGLSGRDLETMAIGLYEGLDENFLRYRIGQLEYLAAKLD
                     KAGIAYQAPVGGHGLFIDAKAMFPHIPYYEFPAQALAIELYKEAGIRSCDIGSYMLGN
                     DPDTGEQLKSEFEFTRLAVPRRVYTQAHFDIMAEALIAIKERAASVKGYRITWEPKIL
                     RHFQASLEPIE"
     gene            complement(2600..3562)
                     /locus_tag="PLB33_08850"
     CDS             complement(2600..3562)
                     /locus_tag="PLB33_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOG63675.1"
                     /translation="MRRILITGALGQIGSELTEEMRKIYGSDNVVASSRRIKEGHEKL
                     IESGPFEIINVNDPKTLASAVDKYKINTIVNLAAVLSAVGEKEPQQCWNINMNGLYNI
                     LEIAKEKNCMVYTPSSIAAFGPSTPKDNTPQDTIQRPTTMYGVTKVAGELLCDYYYKR
                     FGVDTRGVRFPGLISYKTLPGGGTTDYAVHIYYEALKNKKYTSFINKGTYMDMMYMPD
                     ALRAIIDLIEANPDRLKHRNAFNVSAMSFEPEMLAESIKKFIPEFKLEYDVDPVRQEI
                     AESWPNSLDDSDAREEWDWNPQYDLEAMTKDMLEKLRIKLNIQE"
BASE COUNT         1117 a          679 c          574 g         1355 t
ORIGIN      
        1 aaactaaaat ttttttcaat tcttttttca taattcgcct cctaataatt ttttttattt
       61 ttgataaagt caggatgtca atttatatcc gacgcttatc cctcctttct catgcataaa
      121 aatttctatc cggttgatgt caaaataaaa actgtatgcc aaggcataca gtttaagtac
      181 caaaatacac taatacataa tgcattagtt catcactgtt cggcaacctg gcaatcatcg
      241 catttcacat tagacccatg gctttgcgtc cttgcctttc gacaagtttg ctattatcgt
      301 aggataatat taaatttttc tatttgtgca tttattttac tatatttgat ataattatga
      361 aagatttttt ttaaaaataa atagaatttt gttgaatttc ttttgactaa tttcgattta
      421 tttcgtagta ttaactgaag aattgttaaa actctatttc ttctctatgc ttatgatgac
      481 agattaattg ggcgtaaact gtaattgctc gatgtagcga taatatgaaa ggtaaaattt
      541 attgtaaatg tagatgattg ttgatataat taacaaagat attaattatt aaggagtgtt
      601 tatgggcaaa aaaattagag ttaagccaag taaggctcaa tcaactgccg cattttttgt
      661 tggaattgta ttttgtttaa taggtttatt tgttgcaata ccgatggcag gtccatttgg
      721 catattttgg actcttgtag cagttatcat tacggttatg catggcaaaa atgcttttac
      781 tgaagaggga gtttcaactc atgaaataat aatagaagat aatgatttta ttaaaaaaac
      841 taaattaatt aataataccg ctgaagaaac agaaaatgaa gattatgtaa atatttcaga
      901 ttctaaaaaa agactggaag aaataaagct tttgtatgaa aatggtttaa taaacaaaga
      961 tgagtatgaa gaaaaacgaa aaagtattat cgacaatatt taataattca taatttcggg
     1021 gacagacttt aataaagtcg tccccggtat tagaatgata ttatgctatt cgataggctc
     1081 caggcttgct tggaaatgac gaagtatttt tggttcccaa gtaatcctgt atcctttaac
     1141 acttgctgct ctttccttta ttgctattaa agcttctgcc attatatcga aatgagcttg
     1201 agtatacact cttcttggaa ctgccagcct tgtaaattca aattcagatt ttaactgttc
     1261 tcccgtatca ggatcgttgc ctaacatata tgaaccgata tcgcaggatc tgattcctgc
     1321 ttctttgtat aattcaattg ctaaagcttg tgccgggaat tcataataag gaatatgcgg
     1381 gaacatagcc tttgcatcta tgaataatcc gtgcccgcca accggtgctt gatatgctat
     1441 acctgcttta tctaattttg ctgctaagta ttccaattgt cctatcctat atcgtaagaa
     1501 attttcatca agaccttcgt atagaccaat tgccattgtt tccaaatctc tgccggataa
     1561 tccgccgtat gttgtaaagc cttcaaatga aatacagttg gcttttactt tttgataaaa
     1621 ctcttgattg tcttccttga ttcctatcat accgcccata ttaataattg catctttctt
     1681 agcactcatt gtaaagaaat ccgcatagct gaacatttct cttgttatat cttttattga
     1741 ccagtctttg aattcttctt ccctttgctt aacaaagtat gcattttcag cgaatctggc
     1801 agcatcgata tctaatggaa tattatattt cttacataca gccgatacat ctctcatgtt
     1861 ctgaactgat acaggctgtc cgccggctga gttattggtt atagtcatta caactacacc
     1921 tacattttct cttccaagct cgttaataag tttttctaac ttttcaacat ccatatttcc
     1981 tttaaaggga gctcttaaag taggatcctt agcttcttcc actacacaat caattgctct
     2041 tgcaccggca atttccacat gggctctggt tgtatcaaag aacatatttg caatggcgta
     2101 tttacctttg gataacaaaa tcgggaaaaa tactttttct gcagcacggc cttgatgaac
     2161 cggctgaatg attttgtatc cgaatatgtc ctgaccggct tcaataagct tgtaataact
     2221 agttccgccg gcatatgatt catctccgag cattactcct gcccattggt acgagctcat
     2281 agcatttgta ccgctgtctg tcaataaatc gatgtaaacg tcttccgctt taagattgaa
     2341 cacattataa tttgcttctt taatcttttg ctccctctct tcgcgagata acattttgag
     2401 tggttctacc atcttaattc taaacggttc tgctacatac ttaacatcca tattgttttc
     2461 tccttttcaa ttgttagaaa attcttaatt acaatttatc attttaataa aatttttgca
     2521 atataaaatt gagttaattc acggtaagca ttcatcaaaa atttgggaca ttccttgaag
     2581 gaatgtccca aagtataaat tattcttgta tatttaattt aattcttaat ttttcaagca
     2641 tatcttttgt cattgcttct aagtcatatt gcggattcca atcccattct tctctcgcat
     2701 cggaatcatc taaggaatta ggccatgatt cagctatttc ctgccttacc ggatcaacat
     2761 catattcaag cttaaattca ggtataaatt tttttataga ttcagccaac atttcaggtt
     2821 caaaactcat ggctgaaacg ttaaaagcat tgcggtgttt aagtctgtcc ggatttgctt
     2881 ctattaagtc aattattgct cttagtgcat ccggcatata catcatatcc atatatgttc
     2941 ctttattgat aaagcttgtg tattttttat tcttcaaagc ttcatagtaa atatgtactg
     3001 cgtaatcagt tgttcctccc cccggtaacg tcttgtatga aataagtccc gggaaacgaa
     3061 cacctctagt gtctacaccg aatcttttgt aataatagtc acataataat tcaccggcaa
     3121 ctttagttac tccatacatg gttgtgggtc tttgaattgt atcttgggga gtattgtcct
     3181 ttggggttga aggaccaaaa gctgcaatgg aagatggtgt gtaaaccatg caattttttt
     3241 ctttggcaat ttctaagatg ttgtacagac cgttcatatt aatattccaa cattgttgag
     3301 gttctttttc tccaacggca gacaacacgg ctgcaaggtt tacaatggtg tttattttat
     3361 atttatctac cgctgatgcc aatgttttcg ggtcattgac atttatgatt tcaaatggac
     3421 ctgattcaat tagtttctca tgtccttcct ttattcttct gctgctggct actacattgt
     3481 ctgaaccgta aatctttcgc atttcttcag ttaattcgga gcctatttga cccaatgccc
     3541 ctgttattaa aatccttctc aattaattac ctcccataaa tatactatga tactatataa
     3601 ttatgtatat ataataacat aaaacttata aaatataaac aaaagttttc taaaaaatac
     3661 tattttattc aacaaaaaat aagaattgtc tccaaataga ggacgattat ctgattgatt
     3721 ataaa
//
LOCUS       DAOSHP010000125         3631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_23628_length_3631_cov_9.448826, whole genome shotgun sequence.
ACCESSION   DAOSHP010000125 DAOSHP010000000
VERSION     DAOSHP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23628_length_3631_cov_9.448826"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLB33_08855"
     CDS             <1..231
                     /locus_tag="PLB33_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63676.1"
                     /translation="WSKGIKVIPLCCFGLTISSLPLPFNRWQAANANREDSLNRLMKI
                     IAKEFELKYNEIRWKDTIKKYSLLIKGIEENR"
     regulatory      305..380
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            426..797
                     /locus_tag="PLB33_08860"
     CDS             426..797
                     /locus_tag="PLB33_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63677.1"
                     /translation="MKKRIKKALLLVLIMITVLALFAQSAFAISQKLLDEIDKTNEQI
                     FKEVEKAQEKAEKEALKGKGEEEFEEFLDKLIEKLIESTEEKAAKLIKKAAKEGVTLE
                     KTYTEVEIYDRVVTVDPFYAH"
     gene            882..1784
                     /locus_tag="PLB33_08865"
     CDS             882..1784
                     /locus_tag="PLB33_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG63678.1"
                     /translation="MDGLFIYKDKDYIDQVIGTSTLSLLAKKDNFEIMLYEIKADRPN
                     SITPGDSSDLMEFYYIVEGALEIEDGNTLVKLEKGDYFYVSNIKETVPVRTIEDTKML
                     YISSQPVYNLLYTYTGDLRNLLDKSEQKDKYTHNHSNRVQDLSVKIAHRLGLSSEINY
                     TMALASLFHDIGKFFIPDEILNKPSKLSDEELNYIKMHSTYSSELLKGKFIEDISQIV
                     EQHHERLDGSGYPKGLKGNQIRIEARIIGVADSYDAMTTNRAYRSAMTPKEAIEELKS
                     LAGKTYDKNVVIALEQVLIDEGTL"
     gene            complement(1858..2142)
                     /locus_tag="PLB33_08870"
     CDS             complement(1858..2142)
                     /locus_tag="PLB33_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63679.1"
                     /translation="MSCRDDFDVAGDFGRRRKDDIAGDFGRRCECVFECLFELLEDAF
                     EDRRCRRRNFVSPDVGFDSRRRCDCDCVFECLLELLEAAEDDRRCCSRRF"
     gene            complement(2290..2574)
                     /locus_tag="PLB33_08875"
     CDS             complement(2290..2574)
                     /locus_tag="PLB33_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63680.1"
                     /translation="MFNDFMTLDVLTTFAGLTATTMLIVQFTKFLVKKKFGDSYVRVY
                     SFIIALILTFLFARQGGNAQGIVMTIINAILITIAATGGYEILTDPFARK"
     gene            complement(2665..3564)
                     /locus_tag="PLB33_08880"
     CDS             complement(2665..3564)
                     /locus_tag="PLB33_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG63681.1"
                     /translation="MVTKEIKSVFILLLTAAIWGFAFVAQRVGMGFLGAFTFNGIRFA
                     LGSISLLPVIYFFDKKFKSENSAERVDADIKTTIKSGVIAGCILYIAASLQQVGLIYT
                     TAAKAGFITSLYIVLVPILGIFLKQKTHSTTWLGAITAAVGLYFLSINEGFTIEFGDL
                     LQVIGALFWAVHIQVIDKFVKNVDAIKLSSTQFATCSVLSLITAFIFEDISMSGVVSA
                     IVPLLYGGVMSAGVAYTLQAVGQRYAKPSHAAIALSMEAVFAAIGGILILNETMSLRG
                     YLGCALMLAGMLISQAENIKKPE"
BASE COUNT         1281 a          649 c          635 g         1066 t
ORIGIN      
        1 tggtcaaagg gaataaaggt aattccattg tgctgcttcg gtttaaccat aagcagtctg
       61 cctttgcctt ttaaccgctg gcaggcagct aatgcaaaca gagaagacag tttaaaccgg
      121 cttatgaaaa ttatagctaa agaatttgag cttaaataca atgaaataag gtggaaagat
      181 actattaaaa agtatagctt gcttattaaa ggaatagaag aaaatagata aaaattatgt
      241 cgaaaatgtt tgcataagtt aatatattgt gatattatta aataaatcaa taaaatataa
      301 cttcaaccga taatggcaaa gctaacgaaa gttagtgacg caaagctaca gggcctttaa
      361 aatggcagcc agcttccgaa ggaagttttt tacgttcctt ctttatggtt gaaaaggagg
      421 gtaaaatgaa aaaaagaatt aaaaaagctt tattattggt tttaatcatg attacagttt
      481 tggctttatt tgcacaatca gcgtttgcaa taagtcaaaa actattggac gaaattgata
      541 aaaccaatga acaaattttc aaagaggttg agaaagctca agagaaggca gaaaaagaag
      601 ctcttaaagg taagggtgaa gaagagtttg aagagttttt agacaagtta attgaaaaat
      661 tgattgagag tacagaggaa aaggcagcta agctaattaa aaaagctgca aaagaaggcg
      721 ttacacttga aaagacatac acagaagttg aaatttatga cagagtagta acggtagacc
      781 cattttacgc acactgatta tggtcgggaa tgctgattgc attcccgaaa ttttaaaact
      841 tactttattg aggcaatatg accggcacag gagcagagta tatggacgga ttatttattt
      901 ataaagataa agattacatt gatcaagtta taggtacttc tactttaagt ttactagcca
      961 aaaaggataa ttttgaaatt atgctgtatg aaataaaagc cgataggcct aacagcataa
     1021 ctcccggtga ttcgtcagat ttaatggaat tttactatat tgtagaggga gctcttgaaa
     1081 ttgaagacgg caatactttg gttaagcttg aaaaaggtga ttatttttac gtaagcaaca
     1141 taaaagaaac agtaccggtt agaactatag aagatacaaa gatgttatat atttcttccc
     1201 agcctgttta caatttatta tatacataca ccggcgatct tcgtaacctt ttggacaaat
     1261 ctgagcaaaa agacaaatat actcataatc acagcaaccg agtacaggat ttatcggtta
     1321 aaattgccca cagacttggt ctttcaagtg aaattaatta taccatggct ttagcctcat
     1381 tgtttcatga tatcgggaag ttctttatac ctgatgaaat acttaataag ccgtctaagt
     1441 taagcgacga ggaattaaat tacataaaaa tgcacagtac atatagctct gagcttctta
     1501 aaggaaagtt tatagaagac atatctcaga ttgtagagca gcaccacgaa aggcttgacg
     1561 gttccggata ccccaaaggt ttaaagggta atcaaatacg cattgaagca agaattatag
     1621 gagtggcgga ttcatatgat gccatgacta caaacagagc ttatcgcagt gcaatgactc
     1681 caaaagaagc aattgaagaa ttaaaaagtt tggccggtaa aacatacgac aaaaatgtag
     1741 tcattgcttt ggagcaagta ttaatagatg aagggacttt atagaacaac atagtaatat
     1801 aatagatagg gtatacaaac agcatctgtc ttgatttaga cagatgctgt ttatcagtta
     1861 aaagcgtcga ctgcagcagc ggcggtcatc ttcagcagct tctaataatt caagtaagca
     1921 ttcaaataca cagtcgcagt cgcatcttct gcgggaatca aatcctacgt caggacttac
     1981 gaaattacga cggcggcagc gcctgtcttc aaaagcatct tccagcaatt caaacaagca
     2041 ttcaaataca cattcacatc ttctgccaaa atctccggca atatcgtctt tgcgtcttct
     2101 gccgaaatcg cctgctacat caaaatcatc tctacagctc ataaaaaaca ccttttcatt
     2161 aaaatttagg gccaagccca ttaatatact attcttttaa tgaaaaagtg tgataacttg
     2221 tctatgtgtt ttttgcgcta attaacatgt aatagtgctt aattttaatt gacgttcagt
     2281 atcataccgc tattttcttg caaaggggtc tgttaatatt tcgtagcctc ctgtagcagc
     2341 aatcgttatt agtattgcat ttataattgt cattactata ccttgtgcgt ttcctccctg
     2401 gcgtgcaaac aaaaatgtaa gaattaatgc tattataaag ctgtaaactc tcacataaga
     2461 atcaccaaat ttctttttca caagaaattt cgtgaattgt actatcagca ttgtagttgc
     2521 cgtaagacct gcaaaagtag tcaagacatc caaagtcata aaatcattaa acatatatcc
     2581 acctcttttc atttttagta gattatatgc atcattatca agaatatgaa gggacaagga
     2641 gctacttgca gggctagtcc aaccttattc cggtttttta atattttcgg cttgtgaaat
     2701 aagcattcct gcgagcataa gggcacatcc taagtatcct cttaaagaca ttgtttcatt
     2761 aagaatcaat attcctccta ttgctgcaaa cacagcttcc atacttaaag caatggctgc
     2821 atgagagggc ttggcatatc tttgtcccac agcctgcaat gtgtaagcaa cacctgccga
     2881 cattacaccc ccgtataata acggaactat ggcactaaca actccgctca tacttatatc
     2941 ttcaaaaata aaagcagtta ttaaacttaa aactgaacat gtagcaaatt gagtgctgga
     3001 taattttatt gcatccacat tcttaacgaa tttatctatt acttgtatat ggactgccca
     3061 gaataatgct cctataactt gaagcaggtc accgaattca attgtaaaac cttcatttat
     3121 gcttaaaaaa taaagtccga cagctgccgt tattgcacca agccaagtgg tggaatgggt
     3181 tttttgcttt aaaaatattc ctaatatagg caccaatact atgtataaac ttgtaataaa
     3241 ccccgctttt gccgcagtgg tataaataag tcccacttgc tggagagatg ctgcaatata
     3301 caatatacat cctgctatca ctcctgattt tattgttgtt ttaatgtctg catctactct
     3361 ttcagctgaa ttttccgact taaatttttt gtcaaaaaaa taaattacag gcagcagtga
     3421 aatacttccc aaggcaaatc ttataccgtt aaaagtaaat gcacccagaa agcccatacc
     3481 cacacgttgt gcaacaaatg caaatcccca aatagctgca gtaagcagca aaataaatac
     3541 tgacttaatc tcttttgtta ccataataac tccccttctt agtaataaca acatattaac
     3601 acaaaaaaca aataattaaa acagtaattt t
//
LOCUS       DAOSHP010000126         3473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_25178_length_3473_cov_8.006729, whole genome shotgun sequence.
ACCESSION   DAOSHP010000126 DAOSHP010000000
VERSION     DAOSHP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25178_length_3473_cov_8.006729"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..386)
                     /locus_tag="PLB33_08885"
     CDS             complement(51..386)
                     /locus_tag="PLB33_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63682.1"
                     /translation="MKNNFDLTTADGRKKALQAFDKYGWAISLPLWLVKKALEVFSTS
                     DVNTIEAQRKVAIDLIKSGRDNNVDEMTITMDQTAGLDLASEVEGIPIKCKIGKSGHM
                     VVDVRYKSA"
     gene            complement(793..1500)
                     /locus_tag="PLB33_08890"
     CDS             complement(793..1500)
                     /locus_tag="PLB33_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63683.1"
                     /translation="MKNDDFFNKVVDFAKDKIKETAIEISNDAVDKLRNKAMETSNEA
                     IDMLKNKVCDTINSSMSTNINNTKEVVIKKSRSLNNIDVTDSMTNEEEEIFLDNLTSS
                     LRNVGLSAINDPKQALEVVKTLVHATGEVAKFQELQITKRAEIESIRQQALAKIDKQK
                     SLLMTYLERTFDERKEIFTQHFKVVDAALSSGNIQQLAIGLNSINTLAASSPFKNLVS
                     IESTGKALDDPNTEWDF"
     gene            complement(1518..2498)
                     /locus_tag="PLB33_08895"
     CDS             complement(1518..2498)
                     /locus_tag="PLB33_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63684.1"
                     /translation="MGIIKILKKVSGIQAYQDRREASNIKTNADSKYEDASQKTEQLR
                     ESLNGSIQEFGRMRLESLKSTVGIFISYLKDIEQKNKTSQYDFLKSIDIKDEKIKELE
                     SLDMSASKILQGTIASTAIGAAAVAGVPTAITGAVTALATASTGTAISSLSGVAASNA
                     VLAWLGGGTLAMGGGGVAAGAAVLTAATWTVTGGLAVLTAGLIASAHYSKKLTEAKEY
                     EKEVDIRVAEMEKAWVVMEGIEKRIEELKDVTHKLSSRTVNELKYLTPLIPDFDSTDI
                     YYKEVFQKTGLLIKAMGELAKTALFGEDGNLSESSGLIVGEVQSLLNTQL"
     gene            complement(2520..2879)
                     /locus_tag="PLB33_08900"
     CDS             complement(2520..2879)
                     /locus_tag="PLB33_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63685.1"
                     /translation="MNENVNLIIPLVLLVLLTICILAYYLIKPKKVIEKYDSGEIRKK
                     YFVKSGNKVGIENVYYRDGKINKVKEYKNGKIHGIVTTYYPTGVKYIEANYEEGVLTG
                     EYKIFEEDGNIKEIKKY"
     gene            complement(3067..3327)
                     /locus_tag="PLB33_08905"
     CDS             complement(3067..3327)
                     /locus_tag="PLB33_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63686.1"
                     /translation="MEQVIISTSVIISFLVGAGLSWSLFRMLHQQEIENIKQFETVQN
                     EIITIKESLYKADAENLALKIEMEKIICQAVSIETRGTLLSI"
BASE COUNT          967 a          737 c          514 g         1255 t
ORIGIN      
        1 aggatattga cattgttttc tatggatcga gaaactgtca tgattaaatc ctaagcgctt
       61 ttgtaacgca catcaacaac catatgtcca cttttcccaa ttttacattt aatgggaata
      121 ccttctactt cggaagcaag atcaagacct gccgtttgat ccatggtgat tgtcatctcg
      181 tccacattgt tgtctctacc agacttaata agatcaattg caaccttcct ttgcgcttcg
      241 atcgtattaa catcagacgt tgaaaatact tccaatgctt ttttcacaag ccaaagtggc
      301 aaagatattg cccatccgta tttatcaaaa gcttgcagag ccttcttcct gccatcagca
      361 gtggtaagat caaaattatt tttcatttat tatctcctgt ttgaatacag cggcgctgat
      421 cggtggcgga gcttgcaggg atgccgagcg ttccatgtaa gactgtgaca accattcaag
      481 cgccttgttc ggcgtcctct ataaagatac acctaaaagg ttttgagtat gcttaaagtc
      541 ttaagcctcc atcttttcca tctgtgctac tccgaataaa aggcgcgcta aacgatacag
      601 cagcatcgca gtgtccggcg gaaacagttt tctcagatat aataaatttg aacattagag
      661 aatctgttta ttatcttgag atccccgctg aagcaatcat gatgattgca ccaaaacatt
      721 tcggttcaat ctgtaagatt gaaccagggt aaaggctgat tgatatattc atagtgaccc
      781 tttgttgtcc ttttagaaat cccattcagt attcgggtca tcaagagctt tccccgtact
      841 ttcaatggat accaaatttt taaaagggct agaagcagca agagtattta tactatttaa
      901 gccgatggct aactgttgta tattaccgct tgacaaggca gcgtcaacaa ctttaaagtg
      961 ctgcgtaaat atttcttttc tttcatcaaa tgttctttcc aagtaggtca ttaataatga
     1021 cttttgttta tcaattttag caagagcctg ttggcgaata ctttcaattt ctgctctctt
     1081 agtaatttgt aattcctgaa actttgcaac ttcaccagtt gcgtgtacta atgttttaac
     1141 aacttctaat gcctgcttcg ggtcattaat tgctgacaaa ccaacattcc ttagacttga
     1201 agttaaatta tccaggaaaa tttcttcttc ttcatttgtc atgctgtctg taacatctat
     1261 attatttaaa cttcgacttt ttttaatcac tacttctttt gtattattta tgttagtcga
     1321 catagagcta ttaatcgtgt cacagacctt attttttaac atgtcgattg cttcgttact
     1381 ggtttccatt gctttattcc taagcttatc aaccgcatca ttgctaatct ctatagctgt
     1441 ttcctttatt ttatctttgg caaaatctac tactttattg aaaaaatcat cgtttttcat
     1501 atctaaccct cgataattta aagttgtgta tttagcaatg attgaacctc acccacaatc
     1561 aaaccactac tttcggagag attaccgtct tcgccaaata atgctgtttt tgctaactct
     1621 cccatagctt taataagcaa accagttttt tggaatactt ccttgtaata aatgtctgtt
     1681 gaatcaaaat ctggaattaa gggtgtcaaa tacttcaatt catttacagt tctacttgat
     1741 aatttatggg taacgtcttt taattcttct attcgtttct caatgccctc cataactacc
     1801 caagcttttt ccatttcagc cactcgaata tctacttcct tttcatattc ttttgcttct
     1861 gttaattttt tcgaataatg agctgaagca attaatcctg ccgtgagaac tgccaatcca
     1921 ccagttactg tccaagtagc agcagttaaa acggcggcac cagccgcgac accaccgcca
     1981 cccattgcta atgtaccacc acccaaccat gcaagaactg cattcgaagc tgcaacaccc
     2041 gataaagaac ttattgccgt tccggttgaa gctgtagcta aggcagttac agcacctgta
     2101 attgcagttg gtacacccgc tacagctgct gcgccaatag ccgttgatgc aattgttcct
     2161 tgaagtattt tagacgcgga catgtctaaa ctttcaagct ctttgatttt ttcgtcttta
     2221 atatcaattg attttaaaaa atcatattgg ctggttttgt ttttctgctc tatatctttt
     2281 aaatacgaaa taaaaatacc aaccgtactt tttaagcttt ctaatctcat tcgtccaaac
     2341 tcttgaatgc ttccatttaa agattctctc agctgttcag ttttctggct tgcatcctcg
     2401 tactttgaat cggcgtttgt ttttatatta cttgcttccc ttctgtcttg atacgcttga
     2461 ataccggata ctttttttaa aatttttatt attcccatat tcccctcagc acaaattaat
     2521 taatattttt ttatttcttt aatgtttcca tcctcttcaa atattttata ttcaccggtc
     2581 aggacacctt cttcataatt agcttctata tatttaacac cagttggata ataagttgtc
     2641 acaataccat gtatctttcc gttcttatat tctttaactt tattgatctt tccgtcacga
     2701 taataaacat tttcaatgcc caccttatta cctgatttta caaaatattt tttccttatt
     2761 tctcctgaat cgtatttttc gatgactttt ttaggtttaa ttaaataata agctaaaata
     2821 caaattgtta ataagactaa taatactaat ggaattatca gattaacgtt ttcattcatg
     2881 atatttctcc atgcaaaatt tcaaatcatc taaaatgata ctatctttct caaaacaact
     2941 tctcccactg ctgccacttt aaacaggagc aaatggggac aacctaaagt tgttaagtat
     3001 ctttttgatt gctgacggaa atttacccca ttccgtcgga ttcaaaatga ttttaattat
     3061 aataacttaa atacttaaga gtgtccctct cgtttctatt gatacggctt ggcagattat
     3121 tttttccatc tcaattttta gagctaaatt ttctgcatcg gctttatata gagattcctt
     3181 aatggtgata atttcatttt gaaccgtttc aaattgctta atattttcaa tctcctgttg
     3241 atgaagcatt ctgaataatg accaactcaa tcccgcgcct actaaaaaag atataataac
     3301 cgatgtggat ataattactt gctccataaa ggacctcctt ttttgttttt gatatctctg
     3361 atcaacttaa actttttccc acagctgcca tttcagacag gtggttgccg ccgtcgcatt
     3421 tttacctttc tgagctatac agtctgcatg tcccgcatcg gcatttatgt tga
//
LOCUS       DAOSHP010000127         3461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_25323_length_3461_cov_7.537287, whole genome shotgun sequence.
ACCESSION   DAOSHP010000127 DAOSHP010000000
VERSION     DAOSHP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25323_length_3461_cov_7.537287"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1116
                     /locus_tag="PLB33_08910"
     CDS             385..1116
                     /locus_tag="PLB33_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG63687.1"
                     /translation="MQKNNYYIKTNRIFNHVREYGWIATVLIAIGGLWEPKLGLAVLL
                     VMAGLTITSFFSGRYWCGNVCFHGSLFDKIILPVSRNTKIPDFFKSKPFIIGFFIFFM
                     ANFTRRIITVSQYWGTYDIWDKLGALFSNTYLMVLIVGGALAILINPRTWCQFCPMGT
                     MQKLSHSLGKKLGITKKTEKKVTISDKDRCISCGKCAKVCPFQLEPYKEFSDNNQFDN
                     IDCIKCSTCVANCPLKILSLKKDAA"
     gene            complement(1169..1561)
                     /locus_tag="PLB33_08915"
     CDS             complement(1169..1561)
                     /locus_tag="PLB33_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63688.1"
                     /translation="MRWEPHRSIKETELKINDWINRYKNNNFYQWAITFKDIDEPIGA
                     FGLFVVNENDMTGDIAYIISRKYWGMSIATEALKTVLKFAFEVVGFNRIESYHSVNNP
                     ASGKVMQKAGMKLEGLAEQLQPPCNPRT"
     gene            complement(1717..2940)
                     /locus_tag="PLB33_08920"
     CDS             complement(1717..2940)
                     /locus_tag="PLB33_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG63689.1"
                     /translation="MITDNAYVTILLCSYLGVNNGNYKPYTALQWSELGNKIINSKIG
                     EPAKLLNMDKESIINSIKIREEEADRIVNLLSRGANIAFLLEDLSRKGISIVTRSDIE
                     YPKKLKSTLKKYSPPILFYCGDLSLANKIGIGIVGSRNIDDEGIKFTQLLAKKAVEEK
                     LVVFSGGAKGVDEISETTALNNQGYVVSILADSLSKKIMSKAIRDNILSGKQLLLTAN
                     NPDAPFSVANAMNRNKYIYALSNGTFVVASDYNKGGTWAGAVENIKKGWVNTFVWNNN
                     KYIGNTELIKKGGVGIEDLNNISIKELISNKVKRMDQLNIFDFDKTDKRVPFERENII
                     GENEDEYSSSDYDVYNKAINLMLNLLKEPKSIEEVTASLNITKSQAITWINRALNEGK
                     IIKTNRPVRYVVNNE"
     gene            complement(2941..>3461)
                     /locus_tag="PLB33_08925"
     CDS             complement(2941..>3461)
                     /locus_tag="PLB33_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOG63690.1"
                     /translation="KNKIPESYICQNGLALSSYGDAGWGRIVKQNKYVDKYFSDDLVD
                     ASAEVLKSIIDDNDIVWVTSIPSFRHPELVGSFAKRLANNLKLPYYESIIKTKNVKQQ
                     KELHNSNMQFKNAWESFEVNEVKSGNVLLVDDMVDSRWTFTVCGYKLIEKGSGKVYPF
                     TLSNTAGSGGDD"
BASE COUNT         1066 a          677 c          466 g         1252 t
ORIGIN      
        1 gcatacacat atacactttc acctttaatt tgatatataa gtaaatatct ttttaaaaat
       61 accagtttac gatatttccc ctccggtatc aattggtcaa taaattgtta ccttatacaa
      121 ttcattccat tgctcaatac ttgcttcttt tctcatgttc tctcctataa aaatgttttc
      181 atctacgaca tattcatgtg ttttctttaa tattataaca atgttccaaa attgtccaca
      241 ataaaaacat atgcttacgc tctatacttc caaggaggag acagaagaat acaatcggct
      301 gcaactcctg tgtaaccaag tcacggaacc aaaaaaattc ccggcatatt atattagggt
      361 tatgtgaaaa agcaggaggt tattatgcag aagaataatt attacattaa aactaacaga
      421 atctttaatc acgtaagaga atacgggtgg atagcgacgg tactgattgc aataggcgga
      481 ttatgggagc cgaaattagg attggcagta ttattggtga tggcaggttt gaccataact
      541 tcctttttca gcggaagata ctggtgcggc aatgtatgtt ttcatggcag cttgtttgac
      601 aaaataattt tacctgtaag caggaatacc aagataccgg atttcttcaa atcgaagccc
      661 ttcatcatcg gattttttat attttttatg gctaacttta caaggagaat tataaccgta
      721 tcccaatatt ggggtactta tgatatttgg gataagttag gcgccttatt ctcaaacact
      781 tatttaatgg ttttgattgt aggcggtgcc cttgcaatac ttataaaccc cagaacctgg
      841 tgccagtttt gtcccatggg caccatgcaa aagctctctc attccttagg taaaaaatta
      901 ggaatcacca aaaaaacaga aaagaaagta acaatttcag ataaagacag gtgcataagc
      961 tgcggaaaat gtgcaaaggt gtgcccgttt cagcttgagc cttacaagga attctcggat
     1021 aataaccaat ttgataatat tgattgcata aaatgcagca cctgtgtagc taattgtccc
     1081 ttaaaaattc tttccctcaa aaaagatgca gcataaattc tataaaaaac ggtgccggac
     1141 acaaaagccc ggcatcattt acatatgcct atgttctcgg gttacaagga ggctgcaatt
     1201 gttcagccaa accttccaat ttcatacctg ccttttgcat tacttttcct gatgcaggat
     1261 tatttactga atgataggat tcaattctat taaaccccac aacttcaaac gcaaacttaa
     1321 gcacagtctt cagtgcctcc gttgctatgc tcatccccca gtatttcctg cttataatat
     1381 aggcaatatc acctgtcata tcattttcat taactacaaa caaaccaaac gcacctatag
     1441 gttcatctat atctttgaat gtaatagccc attgataaaa attattattt ttgtatcgat
     1501 taatccaatc atttattttt aattcagttt ctttaatact tctatgaggt tcccatctca
     1561 tatagttcct gtatgattta atttattgac tttgctgttc cttacatctt ctcgaataat
     1621 atccgggatg tttatcattt catcttgttt gtcagttttt attttatgtt ccataacgat
     1681 acctcataaa taaattccat ccaaacggta tctctcctat tcattattga caacatatcg
     1741 aacaggccta ttagttttaa taatcttacc ttcgttgaga gccctattta tccaagtaat
     1801 tgcttgagat tttgttatat ttaatgaagc tgttacttct tctatggatt taggctcctt
     1861 taataaattc aacattaaat taattgcttt attataaaca tcataatcag atgagctata
     1921 ttcatcctca ttttcgccaa taatattttc tctctcaaac ggaacccttt tatccgtctt
     1981 atcaaaatca aaaatattta gctgatccat ccttttaact ttattgctta ttaattcttt
     2041 aatggaaata ttgttaaggt cttctatgcc tactccaccc tttttaatca actcagtatt
     2101 tccaatatat ttgttgttat tccagacaaa tgtatttacc catccttttt taatgttttc
     2161 aacagcacct gcccaagttc cccctttgtt ataatcagat gcaacaacaa atgtaccatt
     2221 tgataatgca tagatatatt tattcctatt catggcattt gcaacactaa aaggagcatc
     2281 aggattattt gcagttaata atagctgttt cccactcaaa atattatcgc gtatagcttt
     2341 actcataatt tttttactta aagagtctgc caatattgat acaacatatc cctgattatt
     2401 taaagcagta gtttcggata tttcatcaac gcctttggct cctcccgaaa atactacaag
     2461 tttttcttct acagcttttt tagctaatag ttgagtgaat tttatcccct catcatcaat
     2521 atttcttgat cctacaattc ctatccctat cttattagct aatgataaat ctccgcaata
     2581 aaacagaatt ggtggggaat atttcttaag tgttgattta agctttttag gatattctat
     2641 atcacttctt gttactattg agattccttt tctgcttaaa tcctctaaca agaaagctat
     2701 atttgctcct cttgaaagca agttaacaat tctatcagct tcttcttctc ttatctttat
     2761 actatttata atgctttctt tatccatatt taataatttt gcaggctctc caatttttga
     2821 gttaataatt ttatttccaa gctcactcca ttgcaaagca gtatacggtt tataattacc
     2881 attattaact ccaaggtagc tacataatag aattgtaaca taagcgttat ctgtaatcat
     2941 ttaatcatct cctccagaac ctgcggtatt cgataaagta aatggatata ctttaccgga
     3001 acctttctca attaatttat atccacaaac tgtaaatgtc catcttgaat caaccatatc
     3061 gtcaacaagt aaaacattac ctgatttaac ttcgttaact tcaaagcttt cccatgcatt
     3121 tttaaactgc atattgctat tatgtaattc tttttgctgt ttaacgtttt tagttttaat
     3181 tatagattca taatatggta attttaaatt atttgcaagt cgttttgcaa aacttcctac
     3241 caattcagga tgcctaaatg atggaatcga agtcacccat actatatcat tatcatctat
     3301 tatactttta agaacttctg ctgatgcatc cactaaatca tcagaaaagt atttatctac
     3361 atatttattc tgttttacaa tcctacccca acctgcatca ccatagctgc ttaaagctaa
     3421 tccgttttga catatatatg attctggtat tttgttttta t
//
LOCUS       DAOSHP010000128         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_25400_length_3453_cov_8.452619, whole genome shotgun sequence.
ACCESSION   DAOSHP010000128 DAOSHP010000000
VERSION     DAOSHP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25400_length_3453_cov_8.452619"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /gene="istA"
                     /locus_tag="PLB33_08930"
     CDS             complement(<1..1039)
                     /gene="istA"
                     /locus_tag="PLB33_08930"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOG63691.1"
                     /translation="MIIEVDIYSAIRLRYTEGESIRSISQSLGISRQTVKKYCEGNTH
                     PDVRKTYERSSAVITSDVKSFILSCFIEDETENLKKQKHTAKRIYDRLVNEQSFTGSY
                     SSVRDAVRRLRAEHAVPPQSDIPLAYDPGEAVQYDWGEATAYLDCKKTKLNLFCARLC
                     NSCDIIVQAYQTQTQEAFLEAQQRAFDFFDGVPRRAIFDNAKVAVKEGFGLHAKPQDK
                     YLSFSAHYAFTLDFCNPAKGNEKGLVENLVGYARRNFFVPVPRVVDIKSLNEQLWQSC
                     LSYRANHKVAGRNQSVSELYAIEKEYLNAIPRFRFDTSKTAYAKVDDFSTVRYDKNNY
                     SVPTRYLRKEVT"
     gene            complement(1166..1372)
                     /locus_tag="PLB33_08935"
                     /pseudo
     CDS             complement(1166..1372)
                     /locus_tag="PLB33_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1383..2072)
                     /locus_tag="PLB33_08940"
     CDS             complement(1383..2072)
                     /locus_tag="PLB33_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63692.1"
                     /translation="MKKRRIVIIFTVILILILAYGYHRFTCNEITDTYDWTTLKNIYI
                     TLDDLLDTYEKQNNNNILDEDEIKNNLIFKEYFHQMNTYEWYLSLSPRLNSISYDIRQ
                     IADIDDGGISKQELEYLINLRDKLKIYNDKINGELEEKSIRRMFGIFRKSNVDELEAV
                     FADLLPKNNDITENDNTDSAKNVIDLTFTPIPKHTLKEGHTKEEWIKGKSIYFGKLSD
                     QIESTLDLYLV"
     gene            complement(2278..3051)
                     /locus_tag="PLB33_08945"
     CDS             complement(2278..3051)
                     /locus_tag="PLB33_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOG63693.1"
                     /translation="MRQYVVDAFTEKVFGGNPAAICIMDEWLPDDMMLNITRENNLSE
                     TAFAVKEGENYILRWFTPGGEIDLCGHATLATAYIIFRFIETEKSELCFQTLSGLLKV
                     VKKDDFYEMDFPAYELKKAGITKAMIDAIGLKPSEAYIARDLLCILESEEEVRNLKPN
                     MEKLKMLEGLLLHVTAKGKEFDCVSRSFAPKLNVNEDPVCGSGHCHIIPYWAEKSGKN
                     KLIAYQASERGGTLFCEYQGDRVKIGGKAALYSTADLHI"
     gene            complement(3068..3265)
                     /locus_tag="PLB33_08950"
     CDS             complement(3068..3265)
                     /locus_tag="PLB33_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63694.1"
                     /translation="MITKDMVILNIVEDYPSTEDIFRSYDEIAGKCTMCHNLFDTLEE
                     FAKLYDISLDDLINKLNRKIN"
     gene            complement(3306..>3453)
                     /locus_tag="PLB33_08955"
     CDS             complement(3306..>3453)
                     /locus_tag="PLB33_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63695.1"
                     /translation="EGCGRLEWWCLDTNKPSIDFYLSLNAQPMDEWTVFRITGDTLTE
                     LAKE"
BASE COUNT         1026 a          699 c          490 g         1238 t
ORIGIN      
        1 ccgtaacttc tttccgaaga tatctcgtag gaacggagta gttgttttta tcataacgca
       61 cagtagaaaa atcatctact tttgcataag cagttttgct ggtatcaaat ctaaatcttg
      121 gtatagcatt taaatattct ttttcaatgg cataaagttc ggatactgat tgattcctgc
      181 ctgctacttt atggtttgcc cgataactta aacatgattg ccataattgt tcgtttaagg
      241 atttaatatc aacaacccgt gggaccggaa caaaaaagtt tctcctggca taaccaacaa
      301 gattttctac taagcccttt tcgttacctt tagcaggatt acaaaaatcc aaagtaaagg
      361 cataatgagc actgaaagat aaatatttgt cctgtggttt agcatgaagc ccaaatcctt
      421 cttttacagc gacttttgca ttgtcgaaga tagctctgcg aggcacaccg tcaaagaaat
      481 caaatgccct ttgctgtgct tctaagaaag cctcctgagt ttgagtttgg taagcttgaa
      541 ctattatgtc acaactgttg catagccttg cacaaaacag attcaatttg gtctttttac
      601 agtcaagata ggcagtagct tcgccccaat catattggac tgcttcgcca ggatcgtaag
      661 ctaaagggat atcactttga ggcggtacag catgctcagc tctgagcctg cggactgcgt
      721 ctctgactga tgaatatgaa cctgtaaaac tttgttcgtt tactaatctg tcgtaaatgc
      781 gtttagcagt atgcttttgt ttcttaagat tctcagtttc atcttcgata aaacaactga
      841 gaatgaatga tttaacatcc gatgtaatta ctgctgaaga tctttcatat gttttgcgta
      901 catcaggatg ggtgttgcct tcacaatatt ttttaacggt ctgcctcgat atgccaagac
      961 tttgtgaaat tgaccggata gattcacctt ctgtatatcg aaggcgtata gcagaataaa
     1021 tgtccacttc tatgatcaca cctttcatcc tccttcaaat gtatatttac attttaaagg
     1081 attttatatg ttaggtggta aactttttca ctgtcattca tcatcaaagt ggcacacttt
     1141 tagagtatca tttacacatt ttatgccact tttcgtatgt cgcaatattt tttagttcgc
     1201 atgtatcaaa gctttctctt gttattacac atatgcttct tggttcatta tcatacaaag
     1261 caaacatctc cccacgctca agatatttgt ctatcataga ttctgactcg tcagcaagca
     1321 agagcaaatc aagaaactgc tttttatcct cttttacaat tttaattttc acagaatacc
     1381 ccctacacta aatataaatc tagtgttgat tctatctggt ctgataattt gccgaaataa
     1441 atgctctttc ctttaatcca ttcttctttt gtgtgaccct cttttaaagt atgcttaggt
     1501 atcggcgtaa aagttaggtc tataacattt ttagcagaat cagtattatc attttctgta
     1561 atatcattat ttttcggtag gagatctgca aaaacagctt ctaattcatc tacatttgat
     1621 ttgcgaaaga ttccgaacat tcttcttata gatttttcct ccagctcacc attaatttta
     1681 tcattatata tttttagttt atcacgcaaa tttataagat actctaattc ttgtttacta
     1741 attcccccgt catcaatatc tgctatttgt ctaatgtcat aacttatgga attaagacgg
     1801 ggagacaagg ataaatacca ttcgtaggta ttcatctggt gaaaatactc cttaaaaatt
     1861 aaattatttt ttatttcatc ttcatctaaa atattattat tattttgctt ttcatatgta
     1921 tctaataaat catcaagtgt aatataaata ttctttaatg tagtccaatc ataagtatca
     1981 gtaatctcat tgcatgtaaa acgatgatat ccataagcca atatgagaat taaaattaca
     2041 gtaaatataa taacaatcct tctctttttc acaacgcgct cctttaaata accataatta
     2101 cattgtaaga aacagtcttt tagtttgtat tcataaagca ttacttacct gcctgaaaaa
     2161 agtatatata tctattacag acgtttattt tttagttttg ttccttatta taccatattt
     2221 agtggcatgt cgtcctagaa accttgttaa catttcttca agcttataat taattaacta
     2281 gatatgtaaa tcagctgttg aatataaggc tgccttccct cctattttta ctctgtcacc
     2341 ttgatattcg cagaataatg tgccgcctct ttcagatgcc tggtaagcaa ttaatttatt
     2401 ctttcctgat ttttctgccc agtaagggat aatatggcaa tgacctgacc cgcacaccgg
     2461 atcttcatta acattaagct taggtgcaaa agatctcgac acacagtcaa attcttttcc
     2521 tttagccgta acatgtaaaa gcaatccttc cagcattttt aacttttcca tatttggttt
     2581 taagtttcgt acctcttcct ctgattctaa tatacagagt aagtctcttg ctatatatgc
     2641 ctctgaaggc tttaatccta tagcatctat cattgctttt gtaattcctg ccttttttag
     2701 ttcataagcc ggaaaatcca tttcatagaa atcatctttt ttaacaacct tcaacaatcc
     2761 gctgagagtt tgaaaacaaa gctcagattt ttctgtttca atgaacctga aaataatata
     2821 agctgttgca agagttgcat gcccgcataa atcaatttct ccgcctggcg taaaccatct
     2881 taatatataa ttctctcctt ctttaaccgc aaaggctgtt tcagacaagt tattttctct
     2941 tgtaatgtta agcatcatat catctggcag ccactcatcc ataatacata ttgctgcggg
     3001 attcccacca aacacttttt ctgtaaaagc atcaactaca tattgcctca tccttacatc
     3061 ccccccatta attaattttt ctattgagtt tattgattaa atcatccaaa cttatatcat
     3121 acaattttgc aaattcttcc aatgtatcga ataaattatg acacatagta cacttgcctg
     3181 ctatttcatc atagcttcta aatatatctt ccgtactggg ataatcttca actatattta
     3241 aaattaccat atcttttgtg atcattaaaa taacctccat aaccctccat aaccctcttt
     3301 ttaaattatt cttttgctaa ttcagtaagg gtatcaccgg ttatacgaaa cacagtccat
     3361 tcatccattg gctgtgcatt caaggacaaa taaaaatcta tgcttggctt gtttgtatcc
     3421 agacaccacc attctaatcg gccgcatcct tct
//
LOCUS       DAOSHP010000129         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_29326_length_3125_cov_9.357980, whole genome shotgun sequence.
ACCESSION   DAOSHP010000129 DAOSHP010000000
VERSION     DAOSHP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29326_length_3125_cov_9.357980"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLB33_08960"
     CDS             <1..656
                     /locus_tag="PLB33_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOG63696.1"
                     /translation="KLGIDIKQSKVAIQGFGNVGRYTVKNVQRQGAKIVAIGEWAPKV
                     GTYAIYNEDGLDFEEMLAYFNEHRNLVDYPKAKMISLDEFWNLDVDILIPAALENAIT
                     KEVAEKIKAKLVCEAANGPTTPEADQVLNERKIPVTPDILTNSGGVLVSYFEWVQNLQ
                     GYYWSEKEVEEKQEVKMVKAFNDVWALKEDKNCTIRQAAYMISVKKVAEVMKIRGWY"
     gene            complement(851..1939)
                     /gene="ytvI"
                     /locus_tag="PLB33_08965"
     CDS             complement(851..1939)
                     /gene="ytvI"
                     /locus_tag="PLB33_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOG63697.1"
                     /translation="MEAYLLTEIDKKKFILDVAFIVTVYAVLYFIFVYVIHWVMPFII
                     GFLIALFLRPVTRFVNKFVKSKGKGVALFVIAMFYGVIALIIWFLVSFLITQITDLIY
                     TMPRLYFNRVEPLLLEFNEWIVTNAKAVSPDMASTISQIITNGINYIADFIKNLSISF
                     VQFATRLISNFPLYLISVIFTIVLSVFISLEYDNITSFFRRQLPERFSDIFEQARKFV
                     TGTLWKMIKSYIIIMMITFVELLIILSILRVNYALPIAAIIAVLDILPVIGTGGIVIP
                     WAIVELILKNYFLGVGLFILYFVVLVVRHIIEPRIVGRQIGLHPIITITAMYAGLRLF
                     SVAGLLMGPVVAITVKYLNDEGKIKLFK"
     gene            2177..3022
                     /locus_tag="PLB33_08970"
     CDS             2177..3022
                     /locus_tag="PLB33_08970"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG63698.1"
                     /translation="MREINVEEITRTVEKLCIESNYYLPEDVKKALEEALEKEESPLG
                     VEILSDILKNQEIARTKDVPICQDTGLAVIFLELGQDVRLVGGNLNEAIDEGVRRGYK
                     KGYLRKSAVDDPFIVRKNTGDNTPAIIHTKVVSGENIKLILAPKGGGSENMSALAMLK
                     PSEGIKGIKKFVLDTVEKAGSNPCPPIIVGIGIGGTIEKTTLIAKQSLLRKVGDHNPN
                     PKVAELEKELLEEINKLGIGPQGFGGRTTALAVNIETFPAHIASMPVAINIQCHVARH
                     QEAII"
     gene            3032..>3125
                     /locus_tag="PLB33_08975"
     CDS             3032..>3125
                     /locus_tag="PLB33_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HOG63699.1"
                     /translation="MAKIINMPLTEEVVNDLKAGDRVLLNGIIYT"
BASE COUNT         1152 a          510 c          596 g          867 t
ORIGIN      
        1 aaaaacttgg aattgatatt aaacaatcta aagttgccat tcagggattt ggaaacgtag
       61 gacgttatac agttaaaaat gttcaaagac aaggagccaa aatagtagcc atcggagaat
      121 gggcacctaa ggttggaaca tatgcaatat ataatgaaga tggcttggat tttgaagaaa
      181 tgttggctta cttcaatgaa cacagaaatt tagttgatta tccgaaagct aaaatgatta
      241 gtcttgatga attctggaat ttggatgttg acatactgat tccggcagca ttggaaaatg
      301 ctataactaa ggaagtagca gaaaaaatta aagctaaact tgtgtgcgaa gcagctaacg
      361 gtcctacaac tcctgaggct gaccaggtac tcaatgaaag aaaaatcccg gttactcccg
      421 atatcttaac taactccggc ggagtattag tttcatactt tgaatgggta caaaatcttc
      481 aaggttatta ttggagtgaa aaagaagtag aagaaaaaca agaagttaaa atggttaaag
      541 catttaacga tgtatgggct cttaaggaag ataaaaattg tacaataaga caagcagctt
      601 acatgatttc agttaaaaaa gtagctgaag taatgaaaat aagaggctgg tattaagatg
      661 ccctaaccct atttttcaga gtgttttttc tgaaaaatag tggtaggtca ttcgcaacga
      721 gtgccaattt tgtacgacga aaagagcaaa caaaattgtg caacgagact gcgggatctc
      781 atgggattct tcgagatacg ctttcaaata aaaaagcttc tttgacaaac gtcaaagaag
      841 cctttttcat ttatttaaac aattttattt ttccttcatc atttaagtat ttaactgtta
      901 tggccaccac aggacccatt aaaagacctg caaccgaaaa cagccttaaa cctgcataca
      961 tagccgtgat agttattata ggatgcaagc ctatctggcg gccgacaatt cttggctcta
     1021 taatatgtct tactacaagc acgacaaaat acaatatgaa taatccgact ccaagaaaat
     1081 agttctttaa tattaattcc actattgccc aagggatgac tataccgccg gttcctataa
     1141 cgggcagaat atcaagcact gcaatgattg cagcaatagg cagggcataa ttgaccctca
     1201 gtattgacaa gattattaat aattcaacaa aggtaatcat cattattatt atgtaggatt
     1261 taatcatttt ccacaaggta cctgtaacga attttcttgc ttgttcaaaa atatcactaa
     1321 atctttccgg cagctgtctt ctgaaaaagg atgttatatt atcgtattcc aggctgatga
     1381 atactgaaag aacaattgta aatattacgg atatcagata cagggggaaa ttggatatta
     1441 accttgtggc aaactgtaca aaggatatag aaagattttt aataaaatcc gctatataat
     1501 tgatgccgtt tgttattatt tgtgaaatgg tacttgccat atccggcgaa accgcttttg
     1561 catttgtaac gatccactca ttaaattcaa gaagcagggg ctcaaccctg ttaaaataca
     1621 acctgggcat tgtataaata agatctgtaa tctgggtgat tagaaaggat acaagaaacc
     1681 atatgattaa tgcgataaca ccataaaaca ttgcaattac aaacagggca acgcctttgc
     1741 ccttgctttt cacgaacttg ttgacaaatc tcgtaacagg tctgagaaac aaggctatta
     1801 aaaaccctat tataaatggc ataacccagt gaatcacata aacaaagata aaatataaaa
     1861 ccgcatatac agtaacaata aaagcgacat ccaatataaa ttttttctta tcgatttcag
     1921 ttaacaaata agcctccata ggggtatctc ctcattgagc tgtccccttt ccttatttta
     1981 gattataata tcattgtata ttatttgcaa tagaataaaa aatattacac caattaacac
     2041 tattggagtt taataccata taataaagaa ttatttaaca gctcttttaa aatttataga
     2101 caatatttaa tattattgat ataaatatat tatgatagac ttggacaaga aggatatgta
     2161 tttataggag cagcaaatga gagaaataaa cgttgaagaa ataacaagga cagtagaaaa
     2221 actatgcata gaatctaatt attatcttcc tgaggatgta aaaaaagcct tggaagaagc
     2281 tttggaaaaa gaggagtctc ccttaggagt ggagatattg tctgatatat tgaagaacca
     2341 ggaaattgca aggacaaagg atgttcccat atgccaggac acaggacttg cagttatttt
     2401 tcttgaactg gggcaggatg tgagattggt tgggggaaac ttaaacgaag caattgatga
     2461 aggagtaaga agaggttata aaaaaggata ccttcgaaaa tccgctgttg acgacccctt
     2521 tattgttaga aaaaataccg gcgataatac tcctgcaatc atacatacaa aagtagtttc
     2581 cggagaaaac atcaagctaa tacttgcgcc aaagggcggc ggaagcgaaa atatgagtgc
     2641 tcttgcaatg ctaaagcctt ctgaaggaat aaaaggtatc aaaaaattcg tattggatac
     2701 agttgaaaaa gcaggttcca atccttgccc tcccataata gtgggaatag gaataggcgg
     2761 aacaatagaa aaaacaacct tgattgcaaa acagtccctt cttagaaaag taggtgacca
     2821 taatccaaat ccaaaggtag ctgaattgga aaaagaactg cttgaagaaa taaacaaatt
     2881 gggtataggg ccccagggct ttggaggaag aacaacagca cttgctgtaa atattgaaac
     2941 cttccctgct catatagcaa gcatgcctgt tgcaataaat attcaatgtc atgttgcaag
     3001 gcatcaagaa gcaataatat aggaggtttt catggctaaa attattaata tgcctttaac
     3061 tgaagaagtt gtaaatgacc ttaaggcagg agacagagtt ttattaaacg gaataatata
     3121 tacag
//
LOCUS       DAOSHP010000130         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_30029_length_3073_cov_8.937044, whole genome shotgun sequence.
ACCESSION   DAOSHP010000130 DAOSHP010000000
VERSION     DAOSHP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30029_length_3073_cov_8.937044"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="PLB33_08980"
     CDS             complement(<1..94)
                     /locus_tag="PLB33_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HOG63700.1"
                     /translation="MEKIINMPLTEEIVNDLKAGDRVLLNGIIYT"
     gene            complement(104..949)
                     /locus_tag="PLB33_08985"
     CDS             complement(104..949)
                     /locus_tag="PLB33_08985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG63701.1"
                     /translation="MREINVEEITRTVERLCIESNYYLPQDVKKALEDAMEKEESPLG
                     VEILFDILKNQEIARTKDVPICQDTGLAVIFLELGQDVRLTGGNLNEAIDEGVRRGYK
                     NGYLRKSAVDDPFIERINTKDNTPAIIHTIIVPGDKIKLILAPKGGGSENMSALAMLK
                     PSDGIKGIKKFVLDTVEKAGSNPCPPIIVGIGIGGTIEKTTLIAKQSLLRKVGDHNPN
                     PKVAELEKELLEEINKLGIGPQGFGGRTTALAVNIETFPAHIASMPVAINIQCHVARH
                     QEAII"
     gene            1187..2275
                     /gene="ytvI"
                     /locus_tag="PLB33_08990"
     CDS             1187..2275
                     /gene="ytvI"
                     /locus_tag="PLB33_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOG63702.1"
                     /translation="MEAYLLTEIDKKKFILDVAFIVTVYAVLYFIFVYVIHWVMPFII
                     GFLIALFLRPVTRFVNKFVKSKGKGVALFVIAMFYGISALIIWFFVSFLITQITDLIY
                     TMPRLYFDRVEPLLLEFNDWIVINTKMVSPDMASTISQIITNGINYIADFIKNLSISF
                     VQLATRIISNFPLYLISVIFTIVLSVFISLEYDNITAFFRRQLPDRFSEIFEQARTFV
                     TGTLWKMIKSYIIIMMITFVELFIILSILRVNYALPIAAIIAVLDILPVIGTGGIVIP
                     WAIVELILKNYFLGVGLFILYFVVLVVRHIIEPRIVGRQIGLHPIITITAMYAGLRLF
                     SVAGLLMGPVVAITVKYLNDEGKINLFK"
     gene            complement(2418..>3073)
                     /locus_tag="PLB33_08995"
     CDS             complement(2418..>3073)
                     /locus_tag="PLB33_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOG63703.1"
                     /translation="KLGIDIKQSKVAIQGFGNVGRYTVKNVQRQGAKIVAIGEWAPKV
                     GTYAIYNEDGLDFEEMLAYFNEHRNLVDYPKAKMISLDEFWDLDVDILIPAALENAIT
                     KEVAEKIKVKLVCEAANGPTTPEADQILNQRGIPVTPDILTNSGGVLVSYFEWVQNLQ
                     GYYWSEKEVEEKQEVKMVKAFNDVWALKEDKNCTIRQAAYMISVKKVAEVMKIRGWY"
BASE COUNT          860 a          584 c          499 g         1130 t
ORIGIN      
        1 ctgtatatat tattccgttt aataaaactc tgtctcctgc cttaaggtca tttactattt
       61 cttccgttaa gggcatatta ataatttttt ccatagcagc ctcctaaatt attgcttcct
      121 gatgccttgc aacatggcat tgaatattta ttgcaacagg catacttgct atgtgagcag
      181 ggaaggtttc aatatttacg gcaagtgctg ttgttcttcc accaaagcct tgcggtccta
      241 tgcccagctt gtttatttct tcaagcagct ctttttccag ttccgccacc tttggatttg
      301 gattatggtc tcctaccttt ctaagaaggg actgttttgc aatcaaggtt gttttttcta
      361 ttgttccgcc tattcctatt cccactatta tgggagggca aggattggaa cctgcttttt
      421 caactgtatc caatacgaat tttttgatgc cttttattcc gtcagaaggc ttcagcattg
      481 caagagcgct catattctcg cttccgccgc cttttggagc taatattagt ttaattttat
      541 ctcctggaac tattatggta tgaattatag caggagtgtt gtctttggta ttaattcttt
      601 ctataaaagg atcatcaaca gcggattttc gaaggtatcc gtttttatac cctcttctta
      661 caccttcgtc aattgcttcg tttaagtttc cgccagtcag tctcacatcc tgtcccagct
      721 caagaaaaat aactgcaagt cctgtgtcct gacatatggg aacatccttt gtccttgcaa
      781 tttcttggtt ttttaatata tcaaacaata tctccactcc taatggagac tcctcttttt
      841 ccatagcatc ttccaaagcc ttttttacat cctgcggaag ataataatta gattctatgc
      901 atagcctttc tactgtcctg gttatttctt caacgttaat ttctctcatt tgctgctcct
      961 ataaatacat acccttcttg tccaagtcca tcataatata tttatatcaa taatattaaa
     1021 tcttgtctat aaattttaaa agagttgtta aaaaattctt tattatatgg cattaaactc
     1081 caatagtgtt aattggtgta atatttttta ttctattgca aataatatac aatgatatta
     1141 taatctaaaa taaggaaagg ggacagctca atgaggagat acccctatgg aggcttattt
     1201 gttaactgaa atcgataaga aaaaatttat attggatgtc gcttttattg ttactgtata
     1261 tgcggtttta tattttatct ttgtttatgt gattcactgg gttatgccat ttataatagg
     1321 gtttttaata gccttgtttc tcagacctgt tacgagattt gtcaacaagt tcgtgaaaag
     1381 caagggcaaa ggcgtagccc tgtttgttat tgcaatgttt tacggcattt cggcattaat
     1441 aatatggttt tttgtatcat ttttaatcac ccagattaca gatcttattt atacaatgcc
     1501 tagattgtat tttgacaggg ttgagcccct gcttcttgaa tttaatgatt ggatagttat
     1561 aaatacaaaa atggtttcgc cggatatggc aagtaccatt tcacaaataa taacaaacgg
     1621 tatcaattat attgcagatt tcataaaaaa tctttctata tcctttgtac agcttgccac
     1681 aagaataata tccaatttcc ccctgtatct gatatccgta atatttacaa ttgttctttc
     1741 agtattcatc agtttggaat acgataatat aacagccttt ttcagaagac agctgccgga
     1801 tagatttagt gaaatttttg aacaggcaag aacattcgtt acaggtacct tgtggaaaat
     1861 gataaaatcc tacataataa tcatgatgat tacctttgtt gaattattta taatcttgtc
     1921 aatactgagg gtaaattatg ccctgcctat tgctgcaatc atagcagtgc ttgatattct
     1981 gcctgttata ggaaccggcg gtatagtcat cccgtgggca attgtggaat taatattaaa
     2041 gaactatttt cttggagtcg gattattcat attgtatttt gtcgtgcttg tagtaagaca
     2101 tattatagag ccaagaattg tcggccgcca gataggcttg catcctataa taactatcac
     2161 agctatgtat gcaggtttaa ggctgttttc ggttgcaggc cttttaatgg gtcctgtggt
     2221 ggcaataaca gtcaagtact taaatgatga aggaaaaata aatcttttta aatagcctct
     2281 tttatcatgg gagcagaaaa ggacctcatc atctattaag catagaaaac ccgcagtttc
     2341 gcataacgat aaggttattc aaataaatga caagtaaaaa gcaaggctgt taaaccttgc
     2401 ttttatttat tgacttatta ataccagcct cttattttca ttacttcagc tactttttta
     2461 actgaaatca tgtaagctgc ttgtcttatt gtacaatttt tatcttcctt aagagcccat
     2521 acatcgttaa atgctttaac cattttaact tcttgttttt cttctacttc tttttcactc
     2581 caataatatc cctggaggtt ttgtacccat tcaaagtatg aaactaatac tcctccggag
     2641 ttagttaaga tatccggagt tacaggaatc cctctttggt tgagtatctg gtcagcttca
     2701 ggagttgtag gaccgttagc tgcttcacac acaagtttaa ctttgatttt ttctgctact
     2761 tctttagtta tagcattttc caatgctgcc ggaatcaata tgtcaacatc caaatcccag
     2821 aattcatcaa gactgatcat tttagctttt ggataatcaa ctaaatttct gtgttcatta
     2881 aagtaggcca acatttcttc aaagtccaag ccatcttcat tatatattgc gtatgtacca
     2941 accttaggcg cccattctcc gatagctact attttagctc cttgcctttg aacattttta
     3001 actgtataac gacctacatt tccaaatccc tgaatggcaa ctttagattg tttaatatca
     3061 attccaagtt ttt
//
LOCUS       DAOSHP010000131         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_30271_length_3056_cov_8.937687, whole genome shotgun sequence.
ACCESSION   DAOSHP010000131 DAOSHP010000000
VERSION     DAOSHP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30271_length_3056_cov_8.937687"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLB33_09000"
     CDS             <1..555
                     /locus_tag="PLB33_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOG63704.1"
                     /translation="KISISRASGSLTFPAKFMMVASMNPCPCGYLGDPTHECSCSQGQ
                     IDKYLGKISGPLLNRIDIQIEVLPVKYDDLKDKRAEESSAVIKKRIVKARNIQRERYR
                     NIGILTNSELSTKGISKFCKVNKESEILLKNAFETLGLSVRAYNKILKVARTIADLEN
                     YENIETKHIAEAIRYRSLDRKYWR"
     gene            557..1678
                     /gene="dprA"
                     /locus_tag="PLB33_09005"
     CDS             557..1678
                     /gene="dprA"
                     /locus_tag="PLB33_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOG63705.1"
                     /translation="MDNLNINQKTLLWLSSGNVSNYNISKLLDYFGSVQNLWENFENE
                     QLNLNFLKVETVNKLSKIKIDFEERILEKLNSEKAQIVTIFDNDYPSKLEQIEGAPYL
                     LYYKGSLEDINNISIAVIGSRKATSYGKWAAEKLTKELSDIGVNIVSGLAVGIDTIAH
                     KTALKYNAKTYGIIGCGINIVYPKNNRELFKQIAENKGAVITEYTFDIQPVAFNFHDR
                     NRIISGLSEGVLVIEAQEKSGTLITAGHAANQGREIFAVPGNIDSLYSKGTNALIRDG
                     AKITVSIDDIAEEILELKERVSLKKTFIDLGTLTDDELKIIDCLKSGKNTIYVLEQET
                     KMETGELLGNLTFLEMKGAIKVSADKISLNLSFPSISNK"
     gene            complement(1667..2893)
                     /locus_tag="PLB33_09010"
     CDS             complement(1667..2893)
                     /locus_tag="PLB33_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOG63706.1"
                     /translation="MTQLFIVFIVFLMIPLLSRFKIQLSYILLIACCILALLSGMGAE
                     AALQSVKNTFTELSSVNTILSVMMVSVLGGLMKHYKILDKIVESIYKIIRNKKIILMV
                     LPALMGVLIVPGGALLSAPFIDEIGEEMKIPAPRRAAINLVFRHIAMFILPFSTGLLV
                     ISAALPEINISKLIFLNLFYVIPTTFVGYFLFIKDIKYENTQKVKLSGRNFLTLLLYT
                     SPIYICVIINSVTGLPFFVALIFSVLAVYLLCDKKQFFSILIKSVNYHTVLTVAAILI
                     MKELILNMEELIILFKSMFNAESSMLSILLVFLVSSVFFGIITGNQTATIAIIVPMIS
                     QLGISTDMIYIYTYFSFACSYAGYYFSPIHLCQAFTIQYMNVTTMELYKEYRLYVIVS
                     FIVPFVSVYLMKFLFV"
BASE COUNT         1170 a          454 c          550 g          882 t
ORIGIN      
        1 aagatttcaa tttcaagagc aagcggttcc cttacatttc ctgcaaagtt catgatggtt
       61 gcttccatga acccatgtcc gtgcgggtat ttaggcgacc ctacacatga gtgctcctgc
      121 agccaaggac aaatcgataa atatttagga aaaatttcag gtcccttgtt gaaccgtatt
      181 gatattcaaa ttgaagtgct gcccgttaaa tatgatgatt taaaagataa gagggctgaa
      241 gaaagttcgg cggtaattaa aaagagaata gtaaaggcga gaaatattca gagagaaaga
      301 tacagaaata ttgggatttt gactaattct gagctttcaa ccaaaggaat atccaagttt
      361 tgcaaagtta ataaggaatc tgaaattttg cttaagaatg cattcgaaac attaggctta
      421 agtgtaagag catacaataa aattctaaag gttgcaagga caattgctga cttggaaaac
      481 tatgaaaata ttgaaacaaa gcatatagcg gaagcaattc ggtacaggag tttagaccgc
      541 aaatactgga ggtaaaatgg ataatttaaa tattaatcaa aaaacattat tatggctttc
      601 atccggcaac gtgtcaaatt acaatatttc aaagctcctc gattatttcg gatcggtaca
      661 aaatttatgg gaaaactttg aaaacgagca attaaatttg aattttctta aagttgaaac
      721 agtaaataag ctttccaaaa ttaaaattga ttttgaagaa agaatattgg agaagttaaa
      781 tagtgaaaag gctcaaatag taactatttt tgataatgat tatccttcta aactagaaca
      841 aattgaaggt gctccttatt tgttatacta caaaggcagt ttagaagata ttaacaatat
      901 atcaattgca gtcataggct ccagaaaagc tacaagctat ggaaaatggg ctgcagagaa
      961 actcacaaag gaattgtcgg atattggtgt aaacatagta agcggtctgg cagtcggaat
     1021 tgatacaatt gctcataaaa cagctttgaa atacaatgcc aaaacatatg gtataatcgg
     1081 ttgcggcata aatattgtat acccgaagaa taacagggag ctttttaaac aaatagccga
     1141 aaataaggga gctgtaatta ccgaatatac ttttgatata caaccggttg cttttaattt
     1201 tcatgacagg aatagaatta tcagcggact ttccgaggga gtattggtca ttgaagctca
     1261 ggaaaaaagc ggtacattga ttacagcggg tcatgcagct aatcagggaa gagaaatatt
     1321 tgcggttccc ggaaatatag acagcctgta cagcaaaggc accaatgctc ttatcagaga
     1381 tggagctaaa ataacagtat caatcgatga tattgcggaa gaaattttag aattgaagga
     1441 aagagtaagt cttaaaaaaa catttattga tttaggcaca ttaaccgatg acgaactaaa
     1501 aattatagac tgtcttaagt caggtaaaaa tacaatatat gtattagagc aggaaacaaa
     1561 aatggaaacc ggggaattgc ttggcaattt aacattcttg gaaatgaaag gtgcaataaa
     1621 ggtgagtgcc gataaaattt ctctgaattt aagtttccct tcgatatcaa acaaataaaa
     1681 atttcatcaa atatactgaa acgaagggca ctatgaaaga aacaattaca tacagcctgt
     1741 attccttata gagctccatt gttgtaacat tcatatattg aattgtgaag gcctgacaca
     1801 agtgaatcgg tgagaaataa taacctgcat atgagcatgc aaaagaaaaa tatgtataga
     1861 tgtaaatcat atcagtagat ataccaagct gagaaatcat cgggacaata attgcaatgg
     1921 tagctgtttg attacccgtt ataattccaa aaaatacgga cgatactaag aataccaata
     1981 aaatcgacaa catgctgctt tctgcattaa acatactttt gaataatatt ataagttctt
     2041 ccatatttaa aataagttct ttcatgatta gaatcgctgc aaccgttaat acagtatggt
     2101 aatttacaga tttaatcaat atagagaaga attgtttttt atcacataat aaatagacag
     2161 ctaatacact gaaaatcaga gcaacaaaaa agggaagacc tgttacggaa ttaataatta
     2221 cacaaatata tattggagat gtataaagta aaagagtcaa aaaatttctt cctgataatt
     2281 ttactttttg tgtattttcg tatttaatat ctttaataaa cagaaaataa ccaacaaatg
     2341 ttgttggtat aacgtaaaac aggtttaaaa aaataagttt tgatatattt atttccggaa
     2401 gagcagcgga aataaccaat agtccggttg agaagggcag tataaacatt gcaatatgtc
     2461 tgaataccaa attgatagct gctcgtctgg gagcaggaat tttcatttct tcgccgattt
     2521 catctatgaa aggtgcggat aaaagggcac caccgggaac tattaataca cccattaaag
     2581 caggaagaac catcaatata atttttttgt ttcgtattat cttataaata gattccacta
     2641 ttttatcaag aattttataa tgcttcatta agccgcctaa aacgcttacc atcataactg
     2701 atagtattgt gtttactgat gaaagttccg taaaagtatt ttttaccgac tgcaatgcag
     2761 cttctgctcc cattccggac agcaaagcta aaatacagca tgctatcaaa agaatataac
     2821 ttaactgaat tttaaatcta cttaataaag gtatcattaa gaaaacaatg aatacgataa
     2881 ataattgagt catacgtccc ctccgtgcaa attaacataa ttatataaaa ctattgagaa
     2941 taaatcaata tattattaga catgagcatt cctaaccgca tgataataaa tcttaacaat
     3001 caaaaaggtg aattgacaaa gtaagtgcaa cttttttagt aaaaaagatg gtggat
//
LOCUS       DAOSHP010000132         3022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_30731_length_3022_cov_9.358611, whole genome shotgun sequence.
ACCESSION   DAOSHP010000132 DAOSHP010000000
VERSION     DAOSHP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30731_length_3022_cov_9.358611"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLB33_09015"
     CDS             complement(<1..524)
                     /locus_tag="PLB33_09015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOG63707.1"
                     /translation="MNYIYLGDCIKIMRDDIPENCIDIVYADPPYNLSGKALSLTNNK
                     TGGPFYKMNEDWDTWDYNDYLIFSETWIKKAWNILKNNGSLYISCTYHNIAEIILTAK
                     RAGFKLNNIITWYKTNAMPNITKRTFTHSTEFICWFVKGNKWKFNYEVIKNFNPYKTK
                     DGKPKQLRDFIDFI"
     gene            complement(524..2320)
                     /locus_tag="PLB33_09020"
     CDS             complement(524..2320)
                     /locus_tag="PLB33_09020"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HOG63708.1"
                     /translation="MAKPWSISTTVRNPERLRDFLRVLKKLEGQNFDTANQIKYQVLL
                     IKECIYKPQNIPIPYKKYYEQPEIEVPYNVAEEIFNLQKYKDPPMRGRQSVNPLNKLG
                     FSIAREGYGPVIITELGNKFLSENYDVGYIFFKSMLKLQFPNPWSADFSEKEGFDIMP
                     LIAVMKLIKNINGKAEKKGINRKEFNLLVPTLINYKQIDEYTSRILELRKAKNKDDYI
                     QHFVNKFYGTTTIPAKKMNNLFDYGDNILRYFRLTRYFKVSMGPLGNNWIIDLEPSRK
                     VEIEQLLDMYLGASIKFETVTDYLKYLSDIEQPILPWEKRENLEKVVQTLKLDAINLI
                     MKEKLQISKEQQRFLDIDIHGLDKIQLEQHNSQIRSLNLELKERIKKVKLMSDLNKVE
                     EIISTLQNKKKLREYEPEQFEQLITEALKIINDEILIKPNYPVDDDGEPVSHSPAHKP
                     DIECYYKKFKAIIEVTLNSSKLQWVLEGQPVMRHLRDFEIQHQDDDVFCIFIAPRIHI
                     DTYSQFWISVKYEYNGIPQKIVPMTSEQFAMLLETLLILLKKGIRFSHNELYELYTNI
                     VNESKELLSFSEWAIFIPKSIEDWQQRIIKRC"
     gene            complement(2434..>3022)
                     /locus_tag="PLB33_09025"
     CDS             complement(2434..>3022)
                     /locus_tag="PLB33_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOG63709.1"
                     /translation="LSLNGFPVVIYDTAGLRESPDDIEKEGIAKSYALMQESDLILYL
                     VEANKVTNPNLQIAELLSLSTIPPELYSKTLAVFSKADLLPKNAPEIPEGIYCSVITE
                     KGLKDLTDAISRQLSLSTELLNKPIIINNRHLIALSKCLQFLHQAKHSIEEKQGYEFI
                     AFELISASNALEEILGIITTDDLLNKIFSEFCIGK"
BASE COUNT          902 a          517 c          450 g         1153 t
ORIGIN      
        1 tcaataaagt caataaaatc ccttaattgt ttcggctttc catcttttgt cttgtaagga
       61 ttaaaattct taattacttc ataattgaat ttccatttat taccttttac gaaccagcaa
      121 ataaattccg ttgaatgtgt aaaagttcgt ttagtgatat ttggcatagc attggtctta
      181 taccaagtta taatattatt tagcttgaaa ccggctcttt ttgcagttaa tataatttct
      241 gcgatgttat gatatgtgca ggagatatat aaactgccat tattttttaa aatattccat
      301 gcttttttaa tccaagtttc agaaaagata agataatcat tgtagtccca agtgtcccaa
      361 tcctcattca ttttatagaa cggacctccg gtcttattat ttgttaaaga taatgcttta
      421 cctgacaaat tatatggtgg gtctgcatat actatatcaa tgcaattttc agggatatca
      481 tctctcataa ttttgataca atcgcctaaa taaatatagt tcatcagcat ctttttataa
      541 tcctttgttg ccaatcttca atggacttgg gaataaatat agcccattca gagaaactta
      601 acaattcttt tgattcgtta acaatatttg tatataactc atataattca ttatgcgaaa
      661 atctaatgcc ttttttaagc agtattaaca gtgtctctaa tagcatagca aattgttctg
      721 aagtcattgg gactattttt tgaggaatgc cattatactc atatttaaca gaaatccaaa
      781 attgagaata ggtatcaata tgaatacgag gtgcaataaa aatgcagaac acatcatcat
      841 cctgatgttg aatttcaaaa tctcttaaat gtctcattac cggttgccct tccagcaccc
      901 attgaagttt agatgaattt agagttacct ctataattgc tttgaatttt ttgtaataac
      961 attctatatc cggtttatgg gcaggggaat gagaaactgg ttctccatca tcatctacgg
     1021 gataatttgg ttttattaga atttcgtcat taatgatttt tagcgcttcc gttattaatt
     1081 gttcaaattg ctctggttca tattctctta actttttttt attttgtagc gtacttatta
     1141 tttcttctac tttatttaaa tcactcatta actttacttt cttaattcgt tcttttaatt
     1201 ccaaattgag cgatcttatc tgagaattat gttgctctaa ttgaatttta tccaaaccgt
     1261 gaatgtcaat atctaaaaac ctctgttgtt ctttactaat ttgtaacttc tccttcataa
     1321 ttaaattaat agcatcaagt tttagcgtct ggactacctt ttctaaattc tctcttttct
     1381 cccatggtaa tatgggctgt tcaatatcag aaaggtattt caaataatca gttacagttt
     1441 caaatttaat agatgccccc agatacatat ctaaaagctg ttctatttcc acctttcttg
     1501 acggttcaag atcaataatc caattgttgc ctaacggacc cattgagacc ttaaaatatc
     1561 ttgtaagccg aaaatatctt aatatattat ccccataatc aaacaaatta ttcatctttt
     1621 ttgctggaat agtagtcgtc ccatagaatt tattcacaaa atgctgaata tagtcatctt
     1681 tgttttttgc ttttctcaat tctaaaattc tactcgtata ttcatctatt tgtttataat
     1741 tgataagagt aggaactaac aaattgaatt cttttctatt tattcctttc ttttccgctt
     1801 ttccattgat gttttttatt aatttcatta cggcaattaa aggcattata tcaaaacctt
     1861 ctttctccga aaagtccgca ctccaagggt ttggaaactg aagtttgagc atacttttaa
     1921 aaaatatata tcctacatcg taattttcgg ataaaaattt gtttccaagc tctgtaatta
     1981 taactggtcc atacccttcc cttgctattg aaaatccaag tttatttaat ggattaacag
     2041 attgcctacc tctcattggt gggtctttat atttttgtaa attgaagatt tcttcagcta
     2101 cattgtaagg tacttcaatt tctggctgtt cataatattt tttatacggt atgggaatat
     2161 tctgcggttt gtaaatacat tctttaatta aaagaacttg atattttatc tgattagcag
     2221 tatcaaaatt ttgaccttct aatttcttaa gtactcttaa aaaatctctt aaccgctcag
     2281 ggtttctcac tgtagtcgaa attgaccaag gttttgccat tatctcgcct cttttaatgt
     2341 ttaattttat ttatattcta tgcagtaaac tcgtcaagga ttatttctga aatacaactc
     2401 aaagggcata attactttct gtaaagtggt gggttatttt ccgatgcaga attcgctgaa
     2461 gattttattt aacaggtcgt cggtagtgat aattcctaaa atttcttcca aggcattgct
     2521 ggcggaaata agttcaaagg caataaattc gtagccctgt ttttcttcta ttgagtgttt
     2581 tgcttgatgc agaaactgca ggcatttaga tagagcaatc agatgtctgt tattgatgat
     2641 gataggttta ttcagtaatt cagtgctcag cgataactgc cgggaaattg catcggtaag
     2701 gtctttcaaa cccttttccg ttattacgct gcaataaatt ccctctggta tttcaggtgc
     2761 atttttcggc agcaaatccg ctttactgaa aacggcaagg gttttggaat aaagttcagg
     2821 aggaatagtg gataaagaaa gtaattccgc tatttgcagg ttaggattag ttaccttatt
     2881 agcttccacc aaataaagta taaggtctga ttcctgcatc aatgcgtagc ttttagcgat
     2941 tccttctttt tcaatgtcat caggagattc tcttagtcct gctgtatcat aaataactac
     3001 aggaaatcca tttagggata aa
//
LOCUS       DAOSHP010000133         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_31515_length_2970_cov_6.891938, whole genome shotgun sequence.
ACCESSION   DAOSHP010000133 DAOSHP010000000
VERSION     DAOSHP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31515_length_2970_cov_6.891938"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLB33_09030"
     CDS             complement(<1..482)
                     /locus_tag="PLB33_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005026049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG63710.1"
                     /translation="MEFDMLNLIMKRRSIRKFKDEKLSEKQIQELVNAGILAPTSRNN
                     KAVELIVVDDADTILKLKDCRNMGTKGLQTAPCAIVVIGDSEKSDVWVEDASISASYM
                     QLRAEDLGLGSVWIQMRKRVSDKDSSENEVKKVLNIPDKYGVLCILAIGYKDENKEPY
                     "
     gene            complement(464..595)
                     /locus_tag="PLB33_09035"
     CDS             complement(464..595)
                     /locus_tag="PLB33_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63711.1"
                     /translation="MSKVIIQECKNYSVDDIMGKINSGIEKLGGWKRKRRIQWNLIC"
     gene            complement(601..>1179)
                     /locus_tag="PLB33_09040"
     CDS             complement(601..>1179)
                     /locus_tag="PLB33_09040"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOG63712.1"
                     /translation="DAMGYFSYLPFMECYHDIISMSHKLKGFISVNNKVIDFNEGKGY
                     IEKDWGRSFPNEYIWLQSNHFEGNEASIMFSLAHIPFIGASFQGFICNLIINNHEYRF
                     ATYNKSKIKSVSYSDDYLEIDIVKDKFGLNIKAVVCRDSGELKAPRNGAMDILIKEGL
                     SGAAEIRLLKNSKVVFEGKANPCAIEMMYRKE"
     assembly_gap    1180..1279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1290..1745)
                     /locus_tag="PLB33_09045"
     CDS             complement(1290..1745)
                     /locus_tag="PLB33_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOG63713.1"
                     /translation="MKYDAKTFEEYLAILPEDRKYPFKKLRDIVAENIPKGFKERYSD
                     SFLQFVVPHEIYPNGYHVNPKEPLPFIGIASQKHFIGFYHLGIYAFDDILKWFVEEWP
                     KHVTTKLDMGKSCIRFKKMDQIPYELIAELCQKITVDMWVEKYESSRKK"
     gene            complement(1936..2859)
                     /locus_tag="PLB33_09050"
     CDS             complement(1936..2859)
                     /locus_tag="PLB33_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOG63714.1"
                     /translation="MSTRMDFVRHSLELHLFFARIMKEHSFFLEVAFTPRDSNYTQKA
                     DEFRREFDRLLGEVVSLSNGVVSNSVLQSGEVITPYTQNAEAATSYFTGIRIPTELTQ
                     MEANLMGGGLTGFNPMMEERVYLLNQKAMALISDLIQFKSNILSNVLSCKMFTLNYPL
                     LIDHIMREAKLYLTMIQRLQNREVVNLQQEAAEQEIFWNRIMAEHAKFIRGLLDPTEE
                     QLFEAANNFGNEFDQLTREAVMALNSTVPLQQVTEDSLAATQAIRDFKVQGTQGILDC
                     KIKSIIIPLLGDHTIREANHFIRLLRMYRNI"
BASE COUNT          842 a          592 c          394 g         1042 t
ORIGIN      
        1 ttataaggtt ctttgttttc gtctttatag ccgattgcca atatacaaag aactccatac
       61 ttatcaggta tatttaatac ctttttgact tcattttctg agctgtcttt gtcacttacc
      121 ctctttctca tttgtatcca aaccgagcct aatccgaggt cttcagctct taattgcatg
      181 taggaagctg aaattgatgc atcttctacc catacatcac ttttttcact gtcgccgata
      241 actacaattg cacaaggcgc ggtctgcagt cctttggttc ccatattcct gcaatccttt
      301 aatttaagaa ttgtatcagc atcatcgact acaataagtt caaccgcttt attgttcctt
      361 gatgttggag ctaagattcc ggcattaacc aattcttgga tttgtttttc tgatagtttt
      421 tcatctttaa actttcttat agaccttctt ttcattatta aattcaacat atcaaattcc
      481 attgtatcct ccttttcctt ttccatccgc ctaatttttc aataccactg tttattttac
      541 ccattatatc atctacagaa tagtttttac attcttgaat tataactttg ctcatttaaa
      601 tcattccttt ctgtacatca tttctatagc acatggattt gcttttcctt caaaaacaac
      661 cttcgaattc tttaataatc ttatttctgc tgcaccggat aatccttctt tgattaaaat
      721 atccatagca ccatttctcg gtgcctttag ttcccctgag tcccggcaaa caactgcttt
      781 aatattaagc ccaaacttat ccttaactat atcaatctct aaataatcat ccgaataact
      841 gacgcttttt atcttgcttt tattgtaggt agcaaatctg tactcatgat tattaataat
      901 taaattgcaa ataaaccctt gaaaagatgc tccgataaac ggtatgtggg caagcgagaa
      961 cataatactt gcttcatttc cttcaaaatg attggattga agccatatat attcattagg
     1021 gaatgatcta ccccagtctt tttctatata acctttacct tcattaaaat caataacctt
     1081 gttattaacc gaaataaaac ctttcagctt atggctcatg ctaataatat catgatagca
     1141 ttccataaaa ggaaggtatg aaaaataccc cattgcatcn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnc aattgccatt tatttcttcc tggaactttc atacttttcc
     1321 acccacatat caacggttat tttttgacaa agctctgcaa tcagctcata tggaatttgg
     1381 tccatttttt taaacctgat gcagcttttc cccatatcaa gcttcgtggt aacatgtttt
     1441 ggccattctt caacaaacca ttttaatata tcatcaaaag catatattcc taaatggtaa
     1501 aacccgataa aatgcttctg agacgctatg cctataaaag gaagaggttc ttttggattt
     1561 acatgatacc cattaggata tatttcatga ggaacaacaa attgtaaaaa gctgtctgaa
     1621 tagcgctctt taaaaccctt agggatattt tccgctacaa tatctcttag tttcttaaac
     1681 ggatatttcc tgtcttcggg caagattgcc agatattctt caaatgtttt tgcatcatat
     1741 ttcatgattt ttctcctctt ttataatata acataaatta tacccgctat gatattttag
     1801 aaacataagt atgcagccct ttctacatat attatagccg aatatcattt tattacaatc
     1861 attaacttat ttgggtcaat aaaaaaatag tgcagtatca ttacagatac tacaccgtgc
     1921 tctttccatt tcatactaaa tgtttctata cattcttaaa agtcttataa agtgatttgc
     1981 ttcacgaatt gtatggtcgc cgagaagcgg aattatgatt gatttaatct tgcagtccag
     2041 tatcccttgt gtaccttgga ccttgaaatc ccttattgcc tgagttgccg ccagactgtc
     2101 ttctgtaact tgttgcaagg gcacagttga attcaaagcc attacagcct ctctcgtcag
     2161 ttggtcaaac tcatttccaa aattattggc tgcttcaaac agttgttctt cagttgggtc
     2221 aagcaaccct cttataaact tggcatgctc tgccattatc ctgttccaga atatttcttg
     2281 ctccgcagct tcttgctgca aattaacaac ctctctgttt tgaagccttt gtatcatagt
     2341 taaatacagc ttagcttcac gcataatatg gtcaattaat aagggataat ttagtgtaaa
     2401 catcttgcat gataaaacat tgctcaatat attgctttta aactgtatca aatctgatat
     2461 taatgccatt gctttctggt ttaataaata aaccctttct tccatcattg ggttgaaacc
     2521 ggttaagccg cctcccatca aattagcttc catttgcgta agctctgtgg gaatccttat
     2581 tcctgtaaaa tacgaggttg ctgcttcagc attttgtgta taaggggtta taacttctcc
     2641 tgattgaaga acgctgttgc ttacaactcc atttgaaagt gatacaacct ctcctaagag
     2701 cctgtcaaat tctcttctga attcatctgc tttttgtgta taattactat cccttggggt
     2761 aaatgctact tcaaggaaaa aagagtgttc cttcattatt cttgcaaaaa aaaggtgcaa
     2821 ttctaatgaa tggcgaacaa aatccattct tgtagacatc ataatcctcc ttaaaaattt
     2881 aatgtttaca atatattatg aatcagaaga caaattgctt acaaaatatt taatttatct
     2941 attgacaatt aggtgataat aattatatac
//
LOCUS       DAOSHP010000134         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_36049_length_2709_cov_6.666541, whole genome shotgun sequence.
ACCESSION   DAOSHP010000134 DAOSHP010000000
VERSION     DAOSHP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36049_length_2709_cov_6.666541"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..545
                     /locus_tag="PLB33_09055"
     CDS             183..545
                     /locus_tag="PLB33_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63715.1"
                     /translation="MNKFFKLKVILPIILGVIIGGLLFAFGELDDAPGMCAIGLSVGY
                     ILIMIGINKTGIIKKGLLTPILLLSFAAFLALITTSVLLEGEFGDNPLYSVFGFVVAI
                     VLLLIGLLRIRIFHKSKP"
     gene            complement(645..1937)
                     /locus_tag="PLB33_09060"
     CDS             complement(645..1937)
                     /locus_tag="PLB33_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOG63716.1"
                     /translation="MCAANRIDGTMKFGRAWMIPKEEKLQRGGILMVQDIIKNILIER
                     TVGSENNSTVLLYLEKQLEAMGYDIQKLPFLCTTWETKNSYLKLNERIIGINASPFSE
                     PCDVCGNLAFAKSLEELKGMECQNNILILGGDLVSSPLQPKNYPFYYPDEHRNLIELL
                     EKKQPAAIIAATGKHPLCGLEPFPLFEDGNFLIPSAYISENMLTEIQNSNTQSLIHIS
                     IQSKKIKQNSYQLVAKKKAKKTIGKIVICAHMDSKYNTLGALDNAVGIAVLLEAARKL
                     NSSIFDIDIVPFNGEEYYEASGEVEYLKFIDDNQDKIFFVVNIDSPCHVGSEIAVSLY
                     NLKADTKKLINCLMGERQNVVYGPEWYAGDHAAFAFRGIPCIVLSSSDLFDGALNATH
                     TMKDNIQTVDLSLIEYAADYINDFISVISQKDNPIRCI"
     gene            2311..2571
                     /locus_tag="PLB33_09065"
     CDS             2311..2571
                     /locus_tag="PLB33_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOG63717.1"
                     /translation="MGELLKLTNIGKEIEGQLNKAGIFTYNYLKDIGAKQAWLKILEI
                     DDSACINKLLALEGAIRGIKKSELPPKCKADLKDFYNQHKAK"
BASE COUNT          873 a          443 c          494 g          899 t
ORIGIN      
        1 aaatacattt gaagccctgt ttgaagaagg aataataaaa ccagcataaa tatttgatgg
       61 ataaagctga aaaagtgtca tggcggttat attatcgggg cgaggaaaga aattcgagcg
      121 aaaatcggca gacagccggg taatataaaa aaaattacca tttaagaaag agagaactca
      181 gtatgaacaa attttttaaa ctaaaggtta ttttgcctat cattctgggg gttattattg
      241 gcggactgct ctttgccttt ggtgagttag atgatgcgcc aggaatgtgt gctattggat
      301 tgtctgtagg ttatatccta attatgatcg gaatcaacaa aaccggtatc ataaaaaagg
      361 gactgcttac gccaatttta ctgttgtctt ttgctgcttt cctcgcattg atcaccacat
      421 cagtcctgct tgagggagag tttggtgaca atccgttgta ttccgttttc ggattcgtag
      481 tggcaattgt gttgttacta ataggattat taagaatacg aatatttcac aagtcaaaac
      541 catagggaaa tttagatggg agagattgtt catgtgcgca aagcaatcgc agtaattagc
      601 gggttgacta tttctgtatg ggtatatttt tataacaacc catactaaat gcagcggatt
      661 ggattatctt tttgtgagat taccgaaatg aagtcattga tatagtcagc tgcatattca
      721 attaggctta ggtcaacagt ttgaatgtta tctttcattg tatgagtagc atttaatgct
      781 ccatcaaaca aatcagatga gcttaaaaca atacagggga ttcctctgaa tgcaaatgcg
      841 gcatggtcgc ctgcatacca ttctggacca tagacaacat tttgcctttc tcccatcaag
      901 cagttaataa gcttttttgt atcagctttt aaattataaa gagatacagc tatttcagaa
      961 ccaacatgac aaggagagtc aatatttaca acaaagaaaa ttttgtcttg attgtcatca
     1021 ataaacttca gatattcaac ttctccgctg gcttcatagt attcctcacc attaaacggt
     1081 acaatatcta tatcaaaaat ggaactattc aattttcttg ctgcttccaa taaaactgct
     1141 atacctactg cattgtcaag tgctccaagt gtgttatact tagaatccat atgcgcacat
     1201 atcacgattt ttccaatggt tttttttgct ttcttctttg cgacaagctg ataactattt
     1261 tgctttatct tttttgattg tatcgatata tgtattaagg attgggtatt actattttgg
     1321 atttcggtta acatattttc acttatgtat gcagatggaa tcaaaaaatt accatcttca
     1381 aaaagtggaa atggttccaa gccacatagc gggtgttttc ctgttgctgc aataattgct
     1441 gcgggttgtt ttttctcaag caattcaata agatttctat gttcatccgg atagtaaaaa
     1501 ggatagttct taggctgtag aggactggat accaggtcac cgcctaaaat caaaatattg
     1561 ttttgacatt ccatgccttt taattcttct aaagattttg cgaatgctaa atttccacat
     1621 acgtcacaag gctctgaaaa agggcttgca tttattccga taatgcgttc atttagtttc
     1681 agataggaat ttttcgtttc ccaagtagtg cataaaaaag gcagcttttg tatgtcgtat
     1741 cccatagctt ccagttgttt ttccaaatat aaaagaactg tgctgttgtt ttccgaacct
     1801 acagtgcgtt ctattaatat gttttttatt atatcttgta ccattaaaat tcctcctctc
     1861 tgtaatttct cctctttggg tatcatccaa gcccggccaa atttcatggt gccatcaatg
     1921 cgatttgctg cacacaattt ttgtattttt cgttctgaaa tgcccgtttt ctgaagccgc
     1981 tttcacagaa atgcaatcta tacaatcagc ttcctatcat taaaacaata tacactcatt
     2041 atatacgata ttgcggaaaa cagcaaaaat aaatatttca tgcatgtttt caccttcatt
     2101 tttatgggta aaagtattat atagaaatga aggaagcaaa caattgaaaa cttaaagaat
     2161 attgtattgg acaacaagat tattgccgat tttattcctt tgcttattgt ataataacaa
     2221 aacaatattt atctatgcca caataattca ttgaggggat tcatttgtca aagacgaaaa
     2281 tttggattat actaaacagg agtttttatt atgggggaat tattaaaatt aacgaatatc
     2341 ggaaaagaaa ttgaagggca gttaaataaa gcaggcatat ttacctataa ttatttaaaa
     2401 gatattggag caaaacaagc atggctaaaa atactggaaa ttgatgattc tgcgtgtatt
     2461 aataaattgt tagcactgga aggtgcaatt cgcgggatca agaaatcaga gctaccaccg
     2521 aagtgtaaag ctgaccttaa agatttttat aatcagcata aagctaaata gaaaatcttt
     2581 ctgtgcaagg aatgtggaaa acctgaaaga taagaataaa ctattataaa attcgatatg
     2641 gagggggatt attgtttaat gaaagactta tacgagaaat ttgcttatga ctacgacgaa
     2701 tttggccaa
//
LOCUS       DAOSHP010000135         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_37590_length_2634_cov_9.794106, whole genome shotgun sequence.
ACCESSION   DAOSHP010000135 DAOSHP010000000
VERSION     DAOSHP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37590_length_2634_cov_9.794106"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /gene="rpmE"
                     /locus_tag="PLB33_09070"
     CDS             complement(<1..179)
                     /gene="rpmE"
                     /locus_tag="PLB33_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008338223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOG63718.1"
                     /translation="MKKDIHPNYKTVTVKCACGNTFETGSVLDEIRVEICSECHPFFT
                     GKQKFVDKGGRVDRF"
     gene            complement(281..1675)
                     /gene="rho"
                     /locus_tag="PLB33_09075"
     CDS             complement(281..1675)
                     /gene="rho"
                     /locus_tag="PLB33_09075"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOG63719.1"
                     /translation="MELRNLKLSELKELAKSKGIHDAYKYKKDELILKLNEIESDNND
                     ESKETINDLKIPEDKKETEQEMELPDKIVEEIESSGTEALKVTGILETHSDGYGFLRT
                     NNYLTSDDDVYISPSQIRRFHMQTGDKISGITRPPKQGEKFKALLYVKEINDQNPELA
                     RNRKEFDTLIPVYPDQRIKLEISPIDIAMRIIDLIAPIGRGQRGMIVAPPKAGKTTIL
                     KNIANSIAENYPEIEIIMLLIDERPEEVTDMKRNVKGDVVYSTFDELPKNHIKVAEMV
                     LERAKRLVEHGKDVVILLDSITRLARAYNLTITPTGRTLSGGLDPGALYGPKRFFGAA
                     RNIENGGSLTILASALIETGSRMDDLIFEEFKGTGNMEIHLDRKLSEKRVFPAIDINK
                     SGTRREELLLNNEEMQTIWQLRKAMSNFSTADVTERIIEGLIRTKTNDEFVKQAAQIL
                     NIEKMKADLNDRYY"
     gene            1928..>2634
                     /locus_tag="PLB33_09080"
     CDS             1928..>2634
                     /locus_tag="PLB33_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOG63720.1"
                     /translation="MEKLIITACLTGAEVTKEQQPNLPITPDEIAEAAYECFLAGASM
                     VHVHARDEEGNPTQDYKYYKAIKEKIEAKCNIIFQPSTGGATWHTPEQRMQPIELKPE
                     MATLSMGTCNFGNDVFMNTEENIIKFAKRMYELGVKPELECFEKGMIDTSIRMAKKGI
                     LKTPMHYDFVLGVPGAMGAELRDLLYMVESIPAGSTWTAAAVGRFQLPINMAAIMLGG
                     HVRTGFEDNIYYKKGELA"
BASE COUNT          790 a          484 c          441 g          919 t
ORIGIN      
        1 ttaaatctgt caactcttcc gcctttatca acaaattttt gtttgccggt aaagaaagga
       61 tggcattctg aacatatttc aactctaatt tcgtctaata cagacccggt ttcaaatgta
      121 tttccacatg cacattttac agtaacagtt ttataattgg gatgtatatc ttttttcata
      181 ccgttcacct ctttcttact ctatataaat gtattatctt caattaacct tgagtattat
      241 aacataaggt tttatataat acaatgattt atttttaaaa ttaatagtat ctgtcattta
      301 aatcagcttt cattttttcg atattcaaaa tctgtgcagc ttgttttacg aattcatcat
      361 ttgtcttggt cctaatcaat ccttcaatta ttctttcggt aacatctgca gtagaaaaat
      421 tgctcattgc ttttcttaat tgccatattg tctgcatttc ttcattatta agcagcaatt
      481 cttctcttct tgttccggat ttatttatat caatggcagg aaatactctt ttttccgaaa
      541 gcttcctgtc taagtgaatt tccatattgc cggtgccttt gaattcttca aatattaaat
      601 catccatcct gctgcctgtt tcaattaaag cgctggcaag gattgtaagg cttccgccgt
      661 tttcaatatt tctggcagcg ccaaaaaatc tcttcggacc gtacagagct cccggatcaa
      721 gcccgcctga caaggtcctt cccgtagggg taatagtaag attgtatgct ctggcaagtc
      781 ttgtaatgct gtctaataga ataacaacat ctttaccatg ctcaacaagt cttttcgccc
      841 tttccaatac catttcagct actttaatgt gattttttgg caattcatca aaggttgaat
      901 aaacaacgtc accctttaca tttcgtttca tatccgttac ttcttcggga cgttcatcta
      961 ttaggagcat aataatttca atttcaggat agttttcggc tatactgttt gctatgtttt
     1021 ttaaaattgt tgttttacct gcctttggag gagctacaat catacctctt tgcccgcgtc
     1081 ctatgggggc aattaaatca ataattctca tcgcaatgtc tatgggtgat atttctaact
     1141 tgattctttg atcaggataa acggggataa gcgtatcgaa ctcttttctg ttccttgcta
     1201 attcaggatt ctggtcgttt atttctttaa cataaagcaa tgccttaaat ttttctcctt
     1261 gtttaggagg tcttgtaata cctgaaattt tgtcaccggt ttgcatatga aatcttctga
     1321 tttgagaagg ggatatgtag acatcatcat cgcttgttaa atagttatta gttcttaaaa
     1381 agccgtagcc gtcagaatgg gtttcaagta tgcctgttac ttttaaggct tccgtaccgc
     1441 tggattcaat ttcttcaact attttatcag gcaattccat ttcttgttca gtttcttttt
     1501 tgtcttccgg aattttcaaa tcgttaatcg tttctttact ttcatcatta ttatcagatt
     1561 caatttcgtt taattttaaa atcaattcat ctttcttata tttataggcg tcatgtatgc
     1621 cttttgactt agccaattct tttaattcag aaagctttaa atttctaagc tccatactac
     1681 ctccctattc aattaatcaa cattatggaa aaatacggat aagttatcga cgagtgttta
     1741 aataatatca tttaatatta taatagtcaa gaaaataatt gtttctgtat ttcccttctc
     1801 taagtaataa tactattatt aaatattgtc actgaattat attatgacat gtctttaaca
     1861 atcgattgac tatattgaat ttatacgata aaataaacat aggcaaaaaa ttataatgga
     1921 ggttaaaatg gaaaaattaa ttattactgc ttgtcttacc ggagcagaag taacaaaaga
     1981 gcaacagcct aatttgccga ttacaccgga tgaaattgct gaagctgcat atgaatgctt
     2041 cttggcggga gcatcaatgg tacatgttca tgccagggat gaagagggaa accctactca
     2101 ggactataag tactacaaag caatcaagga aaaaattgaa gcaaagtgca acattatatt
     2161 ccagccatct accggcggag ctacatggca cacaccggag caaagaatgc agccgataga
     2221 attaaaacct gaaatggcaa cactaagcat gggaacatgc aactttggca atgatgtttt
     2281 catgaatact gaagaaaata ttattaaatt tgcaaaacgc atgtatgaac ttggtgttaa
     2341 acctgaattg gaatgttttg aaaagggtat gattgacaca tcaatcagaa tggctaaaaa
     2401 aggcatactt aaaacaccaa tgcactatga ctttgtgctt ggagtgcccg gagcaatggg
     2461 agcagagctc agggacttgt tatacatggt agaatcaatt cctgcaggct ctacatggac
     2521 tgctgcagca gtaggaagat tccagctgcc tataaacatg gcagcaataa tgttaggcgg
     2581 tcatgtaagg acaggttttg aagataatat ttattataaa aaaggcgaac ttgc
//
LOCUS       DAOSHP010000136         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_38438_length_2596_cov_10.475010, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000136 DAOSHP010000000
VERSION     DAOSHP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38438_length_2596_cov_10.475010"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..458
                     /locus_tag="PLB33_09085"
     CDS             138..458
                     /locus_tag="PLB33_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63721.1"
                     /translation="MGKYNDGTLIMPNNYTILNNEEMTYTDGGLSFQPKWWGYYLYLT
                     NNETKLVCTGWKGIAKLPIPSYAKAALKGLSYLFKKKNKSRGIKIRMTGLYTNAVITG
                     CWSR"
     gene            709..1011
                     /locus_tag="PLB33_09090"
     CDS             709..1011
                     /locus_tag="PLB33_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63722.1"
                     /translation="MPKNYAVVNNEEMEYVDGGWSGRQVLKNVIGMTACFSLGYIGNA
                     FAAFVRANQGLSYGKLLAKCGVAAWNFIKALPWQAKVVVGVALGATIWALGQYDLW"
     gene            1115..1783
                     /locus_tag="PLB33_09095"
     CDS             1115..1783
                     /locus_tag="PLB33_09095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOG63723.1"
                     /translation="MIKSNIRYLMIIINFVLAYTHVYKNAVLKADVFSNKNVMEILVY
                     SFVTIISIALALNEIISSVKGFNSDNINDVLSYTILALLISSVFSIFITNNTTNNMYD
                     KSVLVLLINIMISVVLVPIYEEIVFRLAFYKLSSCILNISNNKMLLISINAILFSFLH
                     LINFKDINWIQFIPYFGVYLVLGIATSYIYLKKDNIVFSILLHSFWNGFMYLGSLAVT
                     LTRK"
     gene            1806..2270
                     /locus_tag="PLB33_09100"
     CDS             1806..2270
                     /locus_tag="PLB33_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63724.1"
                     /translation="MNKPIEISNNKTLKLSNVLIKEVNVKDGDDYDTIVLQMENYVKS
                     KGALPIGPLIQKTCYSVNEDGQVELHVFLLRQASNYIHNIESPYKMESVLKVQNCMYA
                     RFVGTEENLRIAHDKINVTAYENNIQLNNENYTVYVDQQDDNIVADIFVEKK"
     gene            2271..>2596
                     /locus_tag="PLB33_09105"
     CDS             2271..>2596
                     /locus_tag="PLB33_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOG63725.1"
                     /translation="MRNSSVKEIRDIKDSRLLYEKDLPPFGYIIVSVITLLVIAVVIW
                     SINTQKSYVIKSQGVIESTNKNYIMSSYTGEINKMNISEGSFVETGDVLFTVKSTDLD
                     LQEEQIT"
BASE COUNT          927 a          351 c          490 g          828 t
ORIGIN      
        1 acgatgatga cactttagtt tacacataag gtgaatacgc tttgtgcaga aaaagtgttc
       61 acaatagatg atatgatctg caacatatca aacccaatag ctgagacata tcttatatgt
      121 cggaaagaaa gtccaaaatg ggaaagtata atgatggcac actgataatg ccaaataatt
      181 acacaattct taataatgag gaaatgactt acaccgatgg cggcttaagc tttcagccca
      241 aatggtgggg atactatctt tacctaacaa acaatgaaac caagcttgtc tgcactggat
      301 ggaaaggaat agccaagctg cctatcccct cctatgcaaa agctgcttta aaagggcttt
      361 cttatctctt caagaagaag aataagagca gaggtatcaa aatacgtatg actgggctgt
      421 atacaaatgc agtcattaca ggatgttggt caagataagc aaagattaaa gaaaccaaac
      481 atagtagtgt atcttctctt atgtaaaagt aagagaagac acatgtatgt ctaaatatcg
      541 gtgatatagt tctatgcgca ggagctttca cataaattca atatgagtgt acagcatatt
      601 gatacaaacg aatataaaca atatgatatg atactaaaaa acttactatg tgaagtgtat
      661 caggaaggac tatttaaaat ggaaatgtgt tatgatggtg cattagtaat gcccaagaac
      721 tacgcagtag ttaataacga agagatggag tatgtcgatg gtggctggtc tggcagacag
      781 gtacttaaaa atgttatcgg catgacagct tgcttctcat taggttatat tggaaatgct
      841 tttgctgcat ttgtaagagc aaatcaggga ctttcctatg gaaagcttct tgctaagtgc
      901 ggtgttgctg cttggaattt tatcaaggca cttccatggc aggcgaaggt tgttgtcgga
      961 gtagctcttg gagcaacaat atgggcatta ggtcagtatg atctttggtg attaagtata
     1021 acgataccta tggctaaata aaagttgggg attctttctt gaatagcatt aagttttcaa
     1081 gaaagaatcc ttgatttata gaaaggaatt tattgtgatt aaatccaata ttcgctattt
     1141 aatgataatc ataaattttg ttttggcgta cactcatgta tataaaaatg cagttttaaa
     1201 agcagatgta ttttcaaaca aaaatgtcat ggaaatatta gtctatagtt ttgtgactat
     1261 tatttcaata gctttagctt taaatgaaat tatcagtagt gtgaaaggct ttaattcaga
     1321 taatatcaat gatgtattat cgtatacaat tttagcattg ctaatatctt ctgtattcag
     1381 cattttcatt accaataata ctacaaataa tatgtatgat aaaagcgtcc ttgtattatt
     1441 gataaacatt atgatatctg ttgtgcttgt gccgatatat gaagaaatcg tttttaggtt
     1501 ggcattttac aaattgagta gttgtatact taatatcagc aataataaaa tgcttctaat
     1561 cagtataaat gctattttgt ttagcttttt gcatttaatt aattttaaag atattaattg
     1621 gattcagttt attccctatt ttggtgtata tttggtttta ggcattgcaa catcatatat
     1681 atatctaaaa aaagataata tagttttttc aatattattg cattcttttt ggaatggttt
     1741 tatgtatctc ggtagtttag ctgttacatt aacaaggaaa taacaaaata ggaaggtaaa
     1801 atattatgaa taagcctatt gaaattagta ataacaaaac attaaagtta tcaaacgttc
     1861 ttatcaaaga ggtaaatgta aaagatgggg atgattatga tacaatagtt ctgcaaatgg
     1921 aaaactacgt aaagtcaaaa ggggcattac ctattggacc tttgattcag aaaacctgtt
     1981 actctgtgaa tgaagatgga caagtagaac ttcatgtgtt tttattgagg caggcatcga
     2041 actatattca taacattgaa tctccatata aaatggaatc tgttcttaaa gtacagaatt
     2101 gtatgtatgc tcgcttcgtg ggaacggaag aaaacctgcg aattgctcat gataaaataa
     2161 atgttaccgc atatgaaaat aatatccaac tgaataatga aaactacaca gtgtatgttg
     2221 atcaacaaga tgacaacatt gttgcagata tttttgtgga gaaaaaataa atgcgtaata
     2281 gttcagttaa agaaattaga gatataaaag atagtcgtct gttatatgaa aaagatttac
     2341 cgcccttcgg gtatattatt gtgtcagtaa ttacattatt agtcatagca gttgtgatat
     2401 ggagcattaa tacacaaaaa tcgtatgtga ttaaaagtca aggagtgatt gaaagcacaa
     2461 acaaaaacta cattatgtct tcatatacag gcgaaataaa caagatgaat atatcagagg
     2521 gaagtttcgt agaaactggc gatgttctat ttacagtgaa aagtaccgat cttgatcttc
     2581 aagaagaaca aatcac
//
LOCUS       DAOSHP010000137         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_38727_length_2582_cov_13.459438, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000137 DAOSHP010000000
VERSION     DAOSHP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38727_length_2582_cov_13.459438"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PLB33_09110"
     CDS             complement(<1..505)
                     /locus_tag="PLB33_09110"
                     /inference="COORDINATES: protein motif:HMM:NF018601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HOG63726.1"
                     /translation="MKYLRRTCCFIDYDYNMSEQSIDQKTEFRYKLKEIISSLIKDYR
                     VNYFISGVGLGFEQSAVEIVLELKEEEYSPLTIEAVLPYESLSIEWNKLQRDKYYSIM
                     QKIDKETLMQYQYTKDCIRKKNIYIINKSDFIIILCKNTCEINDLIRYAKSKKKTLFI
                     IEPDTLNI"
     gene            625..954
                     /locus_tag="PLB33_09115"
     CDS             625..954
                     /locus_tag="PLB33_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOG63727.1"
                     /translation="MIQIGKRLRRHRENMNLTREEFAEKANISPQFLAEIENGKKGMS
                     VDTLYKICKNLSISADYILFGLLSNNALSDSAKNVIGNLPEPLLSYTEDIIQIVNNIV
                     AESKGKY"
     gene            complement(1030..1371)
                     /locus_tag="PLB33_09120"
     CDS             complement(1030..1371)
                     /locus_tag="PLB33_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63728.1"
                     /translation="MKNFTAYIFFTVFGLFLMKLSGQPIEFGIKEGAFFFNISTKMLL
                     ALFFYTASFMLWIGIVAKNDLSFIVPFSSAIVNVLSVLIGVLIFHEHLNTYKAIGIAM
                     AIIGVILMNYK"
     gene            complement(1558..>2582)
                     /locus_tag="PLB33_09125"
     CDS             complement(1558..>2582)
                     /locus_tag="PLB33_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63729.1"
                     /translation="PSVGINVVTQWKDIPFTVGLIWGTLIILKLFFDGNYFGKIINAV
                     EFAACMLIISLFRFNGILVFIVMLLYTFVFVLKSSGSIQKRNYFITTTLILAVFITVN
                     ILVPKQLNAVPNPPGMKLRSIYQGYSAIYVYGGEDELDTKCRKLIETVCTPEQMHEYY
                     NPYFADTISSNTPRFLNNLSKISTKDAIKMYIEGFAAHPGVILGDKFNLTITMWSVTY
                     DRYSYNNAYTTAIQKEMIDEFGVQRKENKFTDIITEYARYTMYENYLSNTLIWRTGFY
                     LSLESILFLYLLLKKDRRISLFIPVICNAIIVFLTMPAQDYRYLWFIFLLFPFLALAC
                     SVSCYE"
BASE COUNT          872 a          432 c          375 g          903 t
ORIGIN      
        1 taatatttaa tgtatcaggc tctattataa ataaagtctt ttttttagat ttagcatatc
       61 gtattaaatc attaatttca catgtatttt tgcatagtat tattatgaag tctgatttat
      121 tgataatata gatatttttc tttcttatac agtcttttgt atattgatat tgcattagtg
      181 tttctttatc aattttttgc atgattgaat aatatttgtc cctttggagc ttattccact
      241 ctatagataa agattcgtag ggcaatactg cttctatggt taatggtgag tattcttctt
      301 cttttaattc aagaacaatt tcaacagccg attgttcaaa cccaagtcct accccgctga
      361 taaaataatt tacgcgataa tcttttatta aactactgat tatttcttta agcttgtacc
      421 taaattcagt tttttgatct atactttgtt ctgacatatt gtaatcatag tcaataaaac
      481 aacatgttct tcttaaatat ttcaccttaa cacctcaaaa ttataattta cgctcatagc
      541 gtactattat aattatagcc tattcaatta tggtttagca atagcttatt atataatagg
      601 gtgataaaat tggatttaaa tattatgata caaataggta aaaggctaag acgacataga
      661 gagaatatga atttgacaag agaagaattt gctgaaaagg ctaatatctc tcctcaattc
      721 ttagctgaaa tcgaaaacgg aaaaaaagga atgtctgtag acactcttta caaaatatgc
      781 aaaaatttat ctatatctgc tgattacata ttatttggtc ttttatcaaa taatgctttg
      841 tccgattcag caaaaaatgt aataggtaat ttgcctgagc ctttattgtc atataccgaa
      901 gatataattc aaattgtaaa taatatagtt gcagaatcta aaggcaaata ttaactaacc
      961 tcctgaattt gccggcgatg gtgtgctttt atgatttgga ccaatatact ttaaactttt
     1021 tacttgcttt tatttgtaat tcatcagaat aacgccaatt attgccattg ctatacctat
     1081 ggctttatat gtatttaaat gttcatgaaa aatgagtacc cctataagta ctgaaagaac
     1141 attaacaatt gcagaagaaa aaggtacaat gaagcttaaa tcattttttg caacaattcc
     1201 aatccataac ataaagctgg cagtgtagaa aaacaatgcc aaaagcattt ttgtacttat
     1261 gttaaagaaa aaagctcctt cttttattcc gaactctata ggctgcccgg atagtttcat
     1321 caagaaaagc ccaaacacag taaaaaatat gtatgctgta aagtttttca tttttcctcc
     1381 attctctgaa tcgttcaaat aatgagattt ttttgtctac gccttctcaa ttatcacgtg
     1441 ttatccacac caaggtatac aaataagtta tgctttgtca tcttgagcgt aagcgaaaga
     1501 tctcatcctt tatgagattc ttcgagctaa agctctcaga atgacactta cgttaggtta
     1561 ttcataacaa ctaactgagc aagctaatgc caaaaatgga aacagcagaa atataaacca
     1621 cagatatctg taatcttgag ccggcatagt cagaaatact atgattgcat tgcatatcac
     1681 agggataaat aagcttattc gtctgtcctt ttttagcaac aaatacagaa ataaaatgct
     1741 ttccaatgaa agataaaaac ctgtacgcca aattaatgta ttggataaat aattttcata
     1801 catggtatat cttgcatatt ccgttataat atcagtaaat ttgttttctt tcctttgcac
     1861 accaaactca tcaatcattt ccttttgtat agcagttgta taggcattgt tatagctgta
     1921 tctgtcatat gtaacagacc acatagttat cgtaagatta aatttatctc caagtatgac
     1981 gccgggatga gctgcaaatc cctcaatgta catttttatt gcatcctttg tgcttatttt
     2041 tgaaagattg tttaaaaacc gaggagtatt acttgaaatt gtatctgcaa aatatggatt
     2101 gtaatactca tgcatctgct ccggagtaca taccgtttct attaattttc ggcattttgt
     2161 atcaagctca tcttcccctc cataaacata aattgcactg taaccttggt atatggacct
     2221 tagcttcatg ccgggaggat taggaactgc atttaattgt tttggaacta atatatttac
     2281 tgtaataaat acagccaaaa taagtgttgt tgttatgaaa taatttcttt tttgaatact
     2341 gccgctgctt ttgagtacaa atacaaaagt gtatagaagc ataacaataa atactaagat
     2401 tccattgaaa cggaacaagg atataatcag catacaagca gcaaattcca cagcatttat
     2461 tattttcccg aaataattac catcaaagaa caatttgagt attatcagcg tgccccaaat
     2521 cagtcccacc gtgaagggta tatccttcca ttgagttaca acatttattc ccacagaagg
     2581 aa
//
LOCUS       DAOSHP010000138         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_38790_length_2579_cov_8.652932, whole genome shotgun sequence.
ACCESSION   DAOSHP010000138 DAOSHP010000000
VERSION     DAOSHP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38790_length_2579_cov_8.652932"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PLB33_09130"
     CDS             <1..665
                     /locus_tag="PLB33_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOG63730.1"
                     /translation="SDLGKKYNIPVIEDIGSGVLADLSSYGLTYEPTVQNSIKAGVDI
                     VCFSGDKLLGGPQAGIIAGKKQYIDKIQKHPLNRALRIDKFTAAVLESIFHEYIDEKR
                     AIKNIPVLKMINLNIEDIKIRAEKLLAKLEDMSEYLDFGVEECLSQIGGGALPLERMK
                     SYAVSIKPKTMSCAKFERELRNSDVPIIGRVVNDTFYIDIRTVLEGEEQIIYDEIKNL
                     FK"
     gene            681..>2579
                     /gene="selB"
                     /locus_tag="PLB33_09135"
     CDS             681..>2579
                     /gene="selB"
                     /locus_tag="PLB33_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOG63731.1"
                     /translation="MRNIIIGTAGHIDHGKTTLIKALTGRETDRWEEEKRRGITIDLG
                     FTYFDLPDGNKAGIIDVPGHEKFIKNMLAGVVGMDMVLLVIAADEGIMPQTTEHINIL
                     NLLGVENGIVVITKCDIVDQEWISLVREDITETISSTFLEGAPIVEVSSKTGQGIEKL
                     IQEIINIADKAVKERELNTIPRLPIDRVFSIQGFGTVITGTLITGILKKGEEVEIYPV
                     NKICRIRNIQVHSSDVEKAYAGQRTAINLSNVKKDEIYRGCVIAPVNSMKNTMMLDVK
                     LNLLKSSKRVVINRSRLHLYTGTSEILTRVVLLDRDELTPGESCYAQLRLEEEIAVRR
                     GDKFIVRFYSPLETIGGGEIIEPLPLKRKRFDENLIEELKIKEKGTGADVIEKIIKET
                     KDLLSVSGLAKTTALTETEVQDNIEILEQEEKITLFKVKNDMYVWHKSFEIEIEEKLE
                     KYLFNYHKENKYATGAKKSEIKSKFFPNLKQLLFDVIIQAFVEKGLIKQSDEFISLTY
                     FTIEYDADYRKIKERVLMILDEVKFELLKIDELSEKINHPMTDDVLSLMLTEKDLVRI
                     NDLVTTRELYEEAKNILVEFLNKNKKISAAQYRDLINTNRKTAIALLEHFDMLKLTKR
                     IENDRILLN"
BASE COUNT         1003 a          339 c          528 g          709 t
ORIGIN      
        1 tatccgattt aggtaaaaaa tataatattc ctgttattga agatatcggg agcggagtgt
       61 tggctgattt aagcagttac gggttgacat atgagcccac ggttcaaaac tcaataaagg
      121 caggagttga tattgtatgt ttcagcggag acaaactttt gggaggtcct caggcaggta
      181 ttattgcagg aaaaaaacaa tatatagata aaatacaaaa acatccttta aacagggcat
      241 tgagaataga caaatttacg gctgcagtat tggaaagtat atttcatgag tatattgacg
      301 aaaaaagagc aataaaaaac atccctgtgc ttaaaatgat aaacttgaat attgaagaca
      361 taaaaatacg tgcagaaaag cttttggcta agcttgaaga tatgtctgag tatttggatt
      421 ttggtgtaga agaatgtttg tctcaaatag gagggggagc acttccttta gaaagaatga
      481 aaagctatgc tgtttccata aaaccaaaaa caatgagctg tgcaaagttt gaaagagaat
      541 taagaaattc tgatgttccg attataggaa gagttgttaa tgatactttc tatatagata
      601 taagaactgt attagaaggt gaagagcaga taatttatga cgagattaag aatttattca
      661 aataatactc tggaggtatt atgagaaata ttattattgg tacagccgga catatagacc
      721 atggtaaaac cacattaata aaagcactga caggaagaga aacagacaga tgggaagaag
      781 aaaagagaag gggaataacc attgatttag gatttactta ttttgacctt cccgatggta
      841 acaaggccgg cataatagat gtgcccggtc atgagaaatt tataaaaaac atgctggccg
      901 gagtagtggg aatggacatg gtgctattgg ttattgcagc cgatgaagga attatgccgc
      961 agaccacaga gcatataaat attttaaact tgttaggcgt ggaaaatgga attgttgtta
     1021 ttactaaatg cgatattgtt gaccaagaat ggataagcct cgttagagaa gatataactg
     1081 aaaccattag ctccacattt ttagaagggg caccgatagt tgaagtatca tccaagacag
     1141 gtcaaggcat agaaaagctt attcaagaaa tcattaatat tgccgataag gcagtaaagg
     1201 agagagaatt aaacacaatt ccaaggcttc ccatagacag agttttttcc atacaaggtt
     1261 ttggtactgt aataacaggt actttgatta caggtatcct aaaaaaggga gaagaagtag
     1321 aaatttatcc tgttaataaa atttgcagga taagaaatat tcaggttcat tcctctgatg
     1381 tggaaaaggc ttatgccggt caaagaaccg caataaactt gtcaaatgtt aaaaaggatg
     1441 aaatttacag gggatgtgtt attgctcctg ttaattcaat gaaaaatacc atgatgctgg
     1501 atgttaagct taatttgcta aaaagctcca agcgagtggt aataaaccgt tcaagacttc
     1561 acctttacac cggtaccagc gaaattctta cacgggtagt gcttctcgac agagatgaat
     1621 taacaccggg agaaagctgc tatgctcagc tcagattaga agaagaaatt gccgtgagaa
     1681 ggggagacaa atttattgta aggttttatt ctcctttaga aacaatcgga ggaggagaaa
     1741 ttattgagcc tttgccttta aaaagaaaaa gatttgatga gaaccttatc gaagagctta
     1801 aaattaaaga aaagggaaca ggtgccgatg ttatagaaaa aataattaag gaaacaaagg
     1861 atttactctc tgtttcgggc ttggcaaaaa caactgccct tacagaaaca gaagttcaag
     1921 acaatattga aatattggaa caggaagaaa aaataactct attcaaagtg aagaatgaca
     1981 tgtatgtatg gcataaaagc tttgaaatcg aaattgaaga aaaattagaa aaatatttgt
     2041 ttaattatca taaagaaaac aaatatgcaa caggagcaaa gaaatctgaa attaaaagca
     2101 aatttttccc caatcttaaa caattattgt ttgatgtaat cattcaggct tttgtagaaa
     2161 agggtttaat aaaacaaagt gatgaattta tatcacttac ttattttaca attgaatatg
     2221 atgcggatta cagaaagatt aaagaaaggg tactaatgat tttagacgaa gttaagttcg
     2281 aattattaaa aatcgatgaa ctgtcagaaa aaattaacca tcccatgaca gatgatgttc
     2341 tttctttaat gttaacagaa aaggatttag taagaataaa tgatttggtt acaacaagag
     2401 aattatatga agaagcaaaa aacatattag ttgaattttt aaataaaaac aaaaaaataa
     2461 gtgcagctca gtacagggat ttgattaata ccaacaggaa gactgcaata gctcttttgg
     2521 aacacttcga tatgttaaaa cttacaaaaa gaatagaaaa tgatagaata ctactaaat
//
LOCUS       DAOSHP010000139         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_39174_length_2562_cov_9.498604, whole genome shotgun sequence.
ACCESSION   DAOSHP010000139 DAOSHP010000000
VERSION     DAOSHP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39174_length_2562_cov_9.498604"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PLB33_09140"
     CDS             complement(<1..799)
                     /locus_tag="PLB33_09140"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOG63732.1"
                     /translation="MNYNFNHIYFIGIGGISMSALAEIMLEEGIKVSGSDRSDSKIIR
                     KLQTSGAKIYLNHDSKHISIDIDLVVYTSAISDDNPELIRARELNIEIMDRAEFLGYV
                     MKNYKNAICVSGTHGKTTTTGMLSSVLIETDLAPTIFLGGEMDSLGGNLLHGSYELLL
                     TESCEYKRNFLKFNPTMEVVLNIEEDHLDYYKDLNDIESAFVEYADKLPPDGYLIVNE
                     SYRHLFDNVKCNIVTFGLDKGAYYARNIKLVPEPSYTLMHGDEEIAEI"
     gene            1293..1760
                     /gene="tsaE"
                     /locus_tag="PLB33_09145"
     CDS             1293..1760
                     /gene="tsaE"
                     /locus_tag="PLB33_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOG63733.1"
                     /translation="MKIIANNLKDTEKLGRIIAKCAEKGTVICLDGELGAGKTSLARF
                     IAKELGVKDYIVSPTFTIIKEYEGRLPFYHMDVYRIDSEDDMYDLGYDEYIYSEGVTV
                     IEWSKLIEGILPEERINIEIKRINDTKREINIDGKGFIFEKLVSELSNENTFN"
     gene            1741..2430
                     /gene="tsaB"
                     /locus_tag="PLB33_09150"
     CDS             1741..2430
                     /gene="tsaB"
                     /locus_tag="PLB33_09150"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOG63734.1"
                     /translation="MKILSIESASVTASCAVSDDEKILGEYTLRHKKTHSEKLMPLIE
                     ELMGELNLKINDIDVISISKGPGSYTGLRIGAAIAKSLAYAADIKIVSIPTMKSLAAN
                     VYDEHKLIVPIMDAKAGRIYTGIYKWDNGECKEVLEQFPCNIDELIQILNEYEEPIIF
                     NGDGSVNYRKRIEDGLNKKAYFAPEVFNYLKASTLAFIGYKMAVQGDIVSANEFNPQY
                     LRLSQAERNRK"
BASE COUNT          878 a          396 c          488 g          800 t
ORIGIN      
        1 taatttcagc tatttcctca tcaccgtgca taagagtata tgaaggctcc gggacaagct
       61 ttatatttcg tgcataataa gcacctttat caagaccgaa ggttactata ttgcatttta
      121 cattatcgaa caaatgcctg tatgattcat tgacaatcaa gtacccgtca ggaggcagtt
      181 tatctgcata ttcaacaaat gcactttcaa tatcatttaa atctttgtaa taatccagat
      241 ggtcttcttc aatattcagt actacctcca tagtggggtt gaatttcaga aagtttcttt
      301 tatattcaca agattcagtc agcagaagtt catatgagcc gtgaagcaag tttcctccca
      361 aggaatccat ttcaccgccc aaaaaaatgg tgggggctaa atcagtttca atcaaaaccg
      421 atgaaagcat tccggtggta gttgttttgc cgtgagttcc ggaaacgcat attgcatttt
      481 tgtaattttt catcacatat cctagaaatt cagctctgtc cattatttca atatttaatt
      541 ccctagctcg tataagctca ggattgtcat ccgaaattgc agatgtatat acaaccaagt
      601 caatatcaat acttatgtgt ttgctgtcat ggtttaagta aatttttgct cctgaagttt
      661 gaagctttct gattattttg gaatcacttc tgtcagagcc cgataccttg attccttcct
      721 caagcattat ttcagctaag gcactcatgc ttattccgcc gattccgata aaatatatat
      781 gattgaagtt ataattcatt gcagtctccc cgggtttcat tattttgttt aatattattc
      841 aattattttg caattaacac agttgtaata aaatttgttg attttgatta ttatatggat
      901 aaatactcat agtattatag catgagtaaa aacattgttc aataaaattt tcttcgatca
      961 tcacagatgg ctatctctat ttaggataag ggcatcgcgg tcgggaaaat agggttaggg
     1021 catctgaaaa atagggttag ggcatcttta tttcttgatt tttaaaatat ataaatgtat
     1081 aatagcaatg ttatgattag aacaaaattg tcatcccgag tgtggtatag gattccaggg
     1141 caatgtcatc ctgagcgaaa gcgaaggatc tcatgagatt cttcgggcta gctctcagaa
     1201 tgacacgtag cttattcata gcgatgacgt tctataagtt atttattgta attacaaata
     1261 tttagtagta aaattataaa gttaggattc atatgaaaat aatagcaaac aatttaaaag
     1321 atacagaaaa gctgggaaga ataatagcaa aatgcgctga aaaaggaaca gtaatttgcc
     1381 tggatggaga attaggagca ggcaaaactt ctcttgcacg ttttattgca aaggaattag
     1441 gagttaaaga ctatattgtc agccctacat ttacaattat taaagaatat gaagggcggc
     1501 ttcctttcta tcatatggat gtgtaccgca ttgactcgga agatgatatg tatgatttgg
     1561 gctacgatga atatatatat tctgagggag ttaccgtaat agaatggtct aagctaatcg
     1621 aagggatact gcccgaagaa agaataaata ttgaaattaa aagaattaat gatacaaaaa
     1681 gagaaataaa cattgatgga aaaggattta tttttgagaa actggtatcg gagttaagca
     1741 atgaaaatac tttcaattga atcagcatct gtaacagctt cttgtgcagt atctgatgat
     1801 gaaaaaattc tgggagaata cacgttaaga cataaaaaaa ctcattcgga aaaacttatg
     1861 cccttaatcg aagaattgat gggagaatta aaccttaaaa ttaacgatat tgatgttatt
     1921 tcaatttcta aaggtcccgg ctcctataca gggttaagaa taggggctgc aattgcaaaa
     1981 agcttggcat atgctgcaga tattaaaatt gtaagcattc caaccatgaa atcactagca
     2041 gccaatgtct acgatgagca caaacttatt gtacctataa tggatgcaaa ggcaggcagg
     2101 atatataccg gcatatataa atgggataat ggagaatgca aggaagtatt agaacaattt
     2161 ccatgcaata tcgatgaatt gattcaaata ctgaatgaat atgaagagcc tataattttt
     2221 aacggtgacg gttccgtaaa ttacagaaaa agaattgagg atggtttaaa caaaaaagca
     2281 tattttgcac ctgaagtttt taattatctt aaagcatcaa cactagcctt tattggctat
     2341 aaaatggcgg ttcaggggga tatcgtctcg gcaaatgaat ttaatccgca atatttgcga
     2401 ttatctcaag cagaaagaaa cagaaagtaa gaggtgcata tgggggaagt tataattagg
     2461 ggaatgcttc ctaaggatat agaccaagta atgaatattg aaagaatttg tttttcattg
     2521 ccatggtcta gggcatcctt tgaaaatgaa ttaaaaaatg aa
//
LOCUS       DAOSHP010000140         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_39258_length_2559_cov_7.416534, whole genome shotgun sequence.
ACCESSION   DAOSHP010000140 DAOSHP010000000
VERSION     DAOSHP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39258_length_2559_cov_7.416534"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLB33_09155"
     CDS             <1..938
                     /locus_tag="PLB33_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOG63735.1"
                     /translation="MAVANVTKRDDNILIVSNGFFGDRFIEICNRKGLNVDVLSAEWG
                     DVVSPEAIEEKLKEKNYAAITVTHVDTSTGARAPIEKIGEVLKKFPDTIYIVDGVAAS
                     AGEREYVDDMNIDILFTGSQKAFGVAPGLAIVWAGKKALARRKSLGTIPEYFIDFEKW
                     IPIMNDPSKYYATPAVNMVWALKESLRLIKEEGLEERYERHAKVSNAFNDSLEAMGFK
                     LLAKKEYRCNTLSTVLYPEGINDAEFRKVLAEEGAVVAGGLAAYAGKMFRIGHMGNID
                     DHTVYSAIAAIERTLIRLGHKYEVGIGLKTLLEKM"
     gene            1187..2503
                     /locus_tag="PLB33_09160"
     CDS             1187..2503
                     /locus_tag="PLB33_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOG63736.1"
                     /translation="MSKPVLSAHFQSRAPSAVRLSQMKYAERKNKPEAVINVGIGNVS
                     LPTNPAMQKRMFNLDAPESPFNKGVIRYTATNGFAETQDAFKHILKCQGFDTSKLSVV
                     VTDGGSAGMELLIMGVCGPAATDEKPLMMIDPSYSNYISFAERIGRKTVTVKRHLNED
                     GKFSLPEIDKIEETIKAYKPGALLVIPYDNPTGQFYDKETLISLAELCVKYNMWMASD
                     EAYRELFYVEGSDLVSIWGITDKDVPGIEGRRISIETASKVWNACGLRIGAVITDSPE
                     FGNRCAAEYTANLCANAIGQYIFGALAHETKEQIAAWCKDIRNYYGDMIKNVTAEFKK
                     LEPDLIVSSPDASIYTVVDVRNVVKPGFDSIEFCLYTAGEGAVDLDGVQTTLLVAPMK
                     GFYDIKKGEHNPGSTQFRLSYVETPENMMKVPKLFVELLKQYEASR"
BASE COUNT          934 a          387 c          538 g          700 t
ORIGIN      
        1 aaatggcagt tgcaaatgtt acaaaaagag atgataatat attgatagta tctaacggat
       61 tttttggtga cagatttatt gaaatctgta acaggaaagg tcttaatgta gatgtattgt
      121 ctgctgaatg gggagatgtt gtttctcctg aagctatcga agaaaaattg aaagaaaaaa
      181 attatgcggc aattaccgta acccatgtgg atacttcaac gggtgcacgg gcaccaattg
      241 aaaaaatagg tgaagtttta aagaaattcc ctgacacaat atacatagtt gacggagttg
      301 cagcttcagc aggtgaaaga gaatatgttg atgatatgaa tattgatatc ttgtttacag
      361 gctctcaaaa ggcatttgga gttgcaccgg gtttagcaat agtatgggca ggaaagaaag
      421 cccttgcaag aagaaaatcc ttaggtacta tccctgaata tttcattgat tttgaaaaat
      481 ggataccgat aatgaacgac ccgtcaaaat attatgctac tcctgccgta aatatggttt
      541 gggctcttaa agaatcatta agacttataa aagaagaagg acttgaagaa agatatgaaa
      601 gacatgcaaa agtatccaat gctttcaacg attctttaga agcaatggga tttaagttat
      661 tggcaaaaaa agaatacaga tgcaatacac tttcaacagt tttgtatcct gaaggaataa
      721 atgatgcaga attcagaaag gtactggctg aagaaggcgc cgtggtagca ggaggacttg
      781 ccgcatatgc aggcaaaatg tttagaatag gacatatggg taatattgat gaccacacgg
      841 tttattcagc aattgcagct atcgaaagaa cattgattag attaggacac aagtatgaag
      901 taggaatagg tttaaaaaca ttgcttgaaa aaatgtaaat aatatataaa gcagctacga
      961 ttagctgctt tatttttgca aataaaaaaa atacccttta ccgattacag ttcacacata
     1021 atagcagcaa atgaatattt ggcgaaacag taacaagcaa tatatattta aaaatgccat
     1081 ataattataa atgtatggac aaaaaaccta aagttcgtta taattatatt taataggtac
     1141 tattaaattt tataaaaata acaatattaa taggagggtt taataaatga gtaaaccagt
     1201 attgtcagca cattttcaat caagagcacc ttcagccgta agactgtcac aaatgaaata
     1261 tgcagagcga aagaataaac ctgaagctgt aatcaatgta ggaatcggta atgtttcatt
     1321 gccaacaaat ccggctatgc aaaaaagaat gtttaactta gatgctcctg aaagcccgtt
     1381 taataagggt gtaatcagat acactgccac caacggattt gcagagacac aggatgcatt
     1441 taaacacatt ttaaaatgtc aaggttttga cacaagcaag ctgagcgttg tagttacaga
     1501 cggcggttca gccggtatgg aattgctaat aatgggagtt tgcggacctg cggcaacaga
     1561 tgaaaaacct ttgatgatga ttgacccatc atacagtaat tatatttctt ttgctgaaag
     1621 aataggacgt aagactgtta cagttaaaag acatttaaat gaagatggaa aattcagcct
     1681 tccggaaatt gacaaaatag aagaaacaat aaaagcttac aagccgggag cacttcttgt
     1741 tataccttat gacaacccta caggccagtt ttatgacaaa gaaacattaa tttcacttgc
     1801 agagctatgc gttaaataca atatgtggat ggcaagtgat gaagcttatc gtgaattatt
     1861 ctatgttgaa ggaagcgatt tagtaagtat ctggggaatt acagataaag atgtgccggg
     1921 tattgaagga agaagaataa gcattgaaac tgcatcaaag gtatggaatg catgcggatt
     1981 aagaatagga gccgttatta ccgacagccc cgaattcggt aatcgctgcg cggcagaata
     2041 tacggcaaac ctatgtgcaa atgcaatcgg acagtatata tttggagcat tggctcatga
     2101 aacaaaagaa caaatagcag cttggtgtaa agacattcgt aattactatg gcgatatgat
     2161 aaaaaatgta acggcagagt ttaaaaaatt agagcccgat ttaattgttt caagccctga
     2221 tgcatcaatt tacactgtag tggatgtcag gaatgttgta aaaccgggat ttgattcaat
     2281 tgaattctgc ctgtatactg ccggagaagg tgctgtagac ttagacggag ttcaaacaac
     2341 actattggtt gctcctatga agggattcta tgatattaaa aagggtgaac acaaccctgg
     2401 aagtactcaa ttccgcttgt catatgttga aactccggaa aatatgatga aagttccaaa
     2461 gttatttgta gagcttttaa aacaatacga agcaagcaga tagtatgaca atgtgatata
     2521 ccttaatcag taagagctga cttattagtc agcccttat
//
LOCUS       DAOSHP010000141         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_39427_length_2551_cov_8.784054, whole genome shotgun sequence.
ACCESSION   DAOSHP010000141 DAOSHP010000000
VERSION     DAOSHP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39427_length_2551_cov_8.784054"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..750
                     /locus_tag="PLB33_09165"
     CDS             46..750
                     /locus_tag="PLB33_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOG63737.1"
                     /translation="MSVSGNERNSCLTIGRMAPDFTALSTDGIVTLSQYRGKWVVIIS
                     EPGNFASTSTSSLIALSTYFDEFAKRNVQILVVTIDNNFSNIEWKMNIFNTYGIIVPF
                     PILEDRDAQIANMYGMVNPDRIYEESVRDIFIINPQGRIKAILTYPVSCGRNTFEILR
                     IIDSLQLTDAYNVYTPANWIPGDPVVLPTTQNFNEAMLRQQGQSGFNCPNWYTCYLDY
                     NSLTQRDNISELKKRS"
     gene            complement(839..1123)
                     /locus_tag="PLB33_09170"
     CDS             complement(839..1123)
                     /locus_tag="PLB33_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOG63738.1"
                     /translation="MYGIHTHGFEGPTTINDNHRHFYEGISSADPNIPGHVHNITATT
                     TFADGHRHSFSVNTAPAVPVRQGHIHPYMGTTTFDDGHVHYFKGNTTVSR"
     gene            1323..1796
                     /locus_tag="PLB33_09175"
     CDS             1323..1796
                     /locus_tag="PLB33_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOG63739.1"
                     /translation="MSNLSDIIESFIKELLDANNNDVIEIQRNILAQQFDCSPSQINY
                     VLTTRFTNDRGYVVESRRGGGGYIRIFRVRSSMEDELKRILNETIGDSITLNKSIDLI
                     NALMEREVITEGERAIMQSVLSDRVLNIVAYEDRNKLRADLLKEMILVSVENNDY"
     gene            1815..2324
                     /locus_tag="PLB33_09180"
     CDS             1815..2324
                     /locus_tag="PLB33_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOG63740.1"
                     /translation="MLCNECGKNEANVHLTHIINGKKTESHLCEECAKKNQTIFNPSF
                     SMQNLFSAMLNNSFNNKTYLPAKGCSNCGMTYENFRTTGKFGCSQCIDSFKGRLKPVI
                     KSIQGYDRHTGKIPKRTGGAYKVQMDIERLKNELRCAVEREEYELAAELRDKIYELEK
                     KKGDEQFEK"
     gene            2314..>2551
                     /locus_tag="PLB33_09185"
     CDS             2314..>2551
                     /locus_tag="PLB33_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOG63741.1"
                     /translation="MKNNDIVITSRIRLARNLKNYKFPIYMTESESKSVINEIYNAIS
                     KHENYKLTYMKDLSDVKKNSLIENHIISPALANKR"
BASE COUNT          928 a          371 c          502 g          750 t
ORIGIN      
        1 agaaaggaga ggcatattta ttattgaata ggccgttagg atattatgag tgttagcgga
       61 aatgaaagaa actcttgctt aacaataggg agaatggctc ctgacttcac tgcactttct
      121 acagacggta ttgtaacatt gtcgcaatac agagggaaat gggtagtgat tataagcgag
      181 cccggaaatt ttgcctctac atcaacctca tctcttatag ccctgtctac gtattttgac
      241 gaatttgcta aaagaaatgt ccaaatatta gttgtaacaa tagataacaa tttttcgaac
      301 atagagtgga aaatgaatat ttttaacaca tatggaataa tagtaccatt tccgatttta
      361 gaggatagag atgcccaaat agcaaatatg tatggaatgg ttaatcctga cagaatatat
      421 gaagaaagtg ttagggatat ttttattatc aatccccaag gaagaataaa agctatattg
      481 acttatccgg tttcttgcgg caggaataca tttgaaattt taagaattat tgattctctt
      541 caactaacgg atgcttacaa tgtttacaca cctgccaact ggatacccgg agaccctgtt
      601 gtgttgccta ctacacaaaa ttttaatgaa gccatgttaa gacagcaagg acaaagcgga
      661 tttaactgtc caaattggta cacttgttat ttagattata attctctaac tcagcgagat
      721 aatatttctg agctgaagaa gagaagttga ttataaaact gatcttagat ttctaattat
      781 tgttcaggat aaaaggcaca aaaaaatcgg cttcataaaa tgatgccgat tattattttt
      841 atcttgaaac tgtagtattc cctttaaaat aatgaacatg cccgtcatca aaggttgttg
      901 ttcccatata cgggtgaata tgcccttgtc ttactggaac tgcaggagcc gtgtttacac
      961 tgaagctgtg tctgtgccca tcagcaaatg ttgttgtagc agttatatta tgaacgtgtc
     1021 ccggaatatt agggtctgca gaagatattc cttcgtaaaa gtgtctatga ttgtcattta
     1081 tggttgtggg accttcaaat ccatgagtat gaatgccata cattatataa tcacctctat
     1141 aataaattaa ttgcttacag tattttatgt cctataatat gctttgttac tgtagactac
     1201 gttttaattg ttattttttt caaaaaaccc cttgcatttt atttttaata gtgttaaaat
     1261 aagtttaagg tcaaagatag tcaaagtcaa agacagtaaa agacaaagat aggaggtgcg
     1321 ggatgtcaaa tttaagtgat ataattgaaa gctttataaa agaattattg gatgcaaaca
     1381 ataatgatgt aatcgaaata caaagaaata ttttggcaca gcagtttgat tgttctccat
     1441 ctcagattaa ttacgttctt actactcgct ttaccaatga caggggatat gttgtagaaa
     1501 gcagaagagg cggaggagga tatatacgaa tttttagggt tcgctcttct atggaggatg
     1561 aactgaagcg catattaaat gaaactatag gagacagcat aacattaaat aaatcaattg
     1621 atttaatcaa tgcactgatg gaaagagaag taataacgga aggtgaaaga gcaattatgc
     1681 aaagtgtatt aagcgacaga gttctaaata tcgttgctta tgaagacagg aataagctgc
     1741 gggccgacct tttaaaggaa atgatattgg tatccgtaga gaataatgat tattgattta
     1801 taggaggggt ataaatgctt tgtaacgaat gcggcaaaaa tgaagccaat gttcatttaa
     1861 ctcacataat caacggaaaa aagacagaaa gccatttgtg tgaagaatgt gcaaaaaaga
     1921 accagacaat ttttaatccg agtttttcaa tgcagaattt gttctctgca atgctgaaca
     1981 attcttttaa taataaaaca tacttacctg caaagggatg ctcaaactgc ggcatgacat
     2041 acgaaaactt cagaactacg ggaaaattcg gatgcagcca atgcattgat tcattcaagg
     2101 gcaggttgaa acctgttata aaaagtatac agggatatga cagacataca gggaaaattc
     2161 ctaaacgaac aggcggagct tacaaggttc aaatggatat tgaaagattg aaaaatgagt
     2221 taagatgtgc cgttgagaga gaggaatatg aacttgcggc ggaacttaga gataaaatct
     2281 acgaattaga aaagaaaaag ggggatgagc agtttgaaaa ataatgatat cgtaataacg
     2341 agcagaatca gacttgcaag aaatttaaaa aattataaat tccctatata tatgaccgag
     2401 tcagaatcaa aaagtgtaat taatgaaatt tataatgcaa taagcaaaca tgaaaattac
     2461 aaattaacat atatgaaaga cttatcagat gtaaagaaaa attcattgat cgaaaaccat
     2521 attatctctc cggcattggc aaataaaagg a
//
LOCUS       DAOSHP010000142         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_39454_length_2550_cov_9.269339, whole genome shotgun sequence.
ACCESSION   DAOSHP010000142 DAOSHP010000000
VERSION     DAOSHP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39454_length_2550_cov_9.269339"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PLB33_09190"
     CDS             <1..1068
                     /locus_tag="PLB33_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOG63742.1"
                     /translation="PRLIVLIVIFVLFLANNIAKYRLKAQNKILISLLVDIALIYILE
                     ANSRLLINYFFHSFYIIIFLEASLLLPLKKGITIGIITVFISMIKYAYLIYYKFNLSN
                     VSQMVFFLMVNILILVIAAFAQHTKDEKEKKDILYRELLDTHKQLKESTDELKRLSAI
                     EERNRIARDIHDTLGHNMTALIMQLQMAEHYAKSDEVKSSQMLNNSLNTAKESLSKIR
                     EVVETLRGNEVTPKMEIKTLVKEFSEKTGAEIELKIEGEISNNHDVNSAIYHILQEGM
                     TNAIRHGNASKIWINLIYSNEYIKFNIKDNGAGALAFIEGFGMKGIRERAESFGGNVE
                     FKSQDGFIIDGFIPLVCHPER"
     gene            1256..1903
                     /locus_tag="PLB33_09195"
     CDS             1256..1903
                     /locus_tag="PLB33_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOG63743.1"
                     /translation="MIKLLLADDQDILTEGLKLLLGAEEDIEIVGTANNGKKAYDLCR
                     IRKPDLVLMDIQMPEVNGVEATAMIKKDFPQTKIIVLTTFNDDEYIYDALKNGASGYM
                     LKDASPKEILEAVRTVYNGGALIQSEVAVKVIDQFSQLAKDTGDKHIDPNVGLLTDRE
                     IEICRLIAEGKNNKEIADELYLSQGTVKNYITKMLLKLDLRDRTQLAIFTIKNNL"
     gene            2156..>2550
                     /locus_tag="PLB33_09200"
     CDS             2156..>2550
                     /locus_tag="PLB33_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63744.1"
                     /translation="MKRKIIFVTVFILILLILLTSCNTNNLIEYKKASDKTEQIIKGQ
                     TAGEFTMTTEINPAGLTAEEIKELNYIKDMNGSFSVVFDDEEEKTIIRNYLNFGGLGY
                     DFEVYINGDEVSVKLPVVGKYLRINEEMLS"
BASE COUNT          945 a          357 c          490 g          758 t
ORIGIN      
        1 ccgaggctga tagtgcttat agtaatcttt gtgctttttt tggccaataa tatagccaaa
       61 taccgtttaa aagctcaaaa taaaatttta atatcattac ttgttgatat agcacttatt
      121 tatattttgg aagcaaattc aaggctttta ataaattatt tttttcactc tttttatata
      181 ataatatttc ttgaagcatc tcttttactg ccccttaaaa aaggaattac aatcggaatt
      241 attacggtat tcatatccat gataaaatac gcatatttga tttattataa atttaatttg
      301 tccaatgtat cccagatggt cttttttctc atggtaaata tattgatttt ggtaattgca
      361 gcatttgcac aacacaccaa ggatgaaaaa gaaaaaaagg atattttgta cagagagctt
      421 ttggatactc acaagcagct taaagaatct accgatgaat taaaaaggct gtccgccatt
      481 gaagaaagaa acagaatagc cagagacata cacgatacct tgggtcacaa tatgacagcc
      541 ttgataatgc agcttcagat ggcagaacat tatgcaaagt ctgatgaggt aaaatcgtca
      601 cagatgctaa ataactcatt aaatactgca aaagaaagct tatctaaaat aagagaagtt
      661 gtggaaactt tgaggggtaa tgaagttaca ccgaaaatgg aaataaaaac tttagttaaa
      721 gaattttccg agaaaaccgg tgcagaaatt gaattgaaaa ttgaaggtga aatatcaaac
      781 aaccacgatg ttaactcggc aatataccac atattgcagg aagggatgac aaatgctata
      841 agacacggca atgcatcaaa aatttggatt aatttaatct attccaatga gtatataaaa
      901 tttaacataa aggataacgg agcaggagct ttggctttta ttgaaggatt tggtatgaaa
      961 ggaataagag aaagagctga atcattcggc gggaatgtcg aatttaaatc gcaagacggt
     1021 tttataatag acggatttat ccctttggta tgtcatcctg agcggtagcg aaggatctaa
     1081 tgaatggcat tattctaaca cagtaaagaa tcctatatgc agtgtcattc tgaacgcagt
     1141 gaagaattct atatgcagtg tcattctgaa cgcagtgaag aattctatat gcagtgtcat
     1201 tctgaacgca gtgaagaatc tcatctttta atggcaaggg aattggagga aattaatgat
     1261 taaattacta ttggcagatg accaggatat tttgactgaa gggcttaagc tgttacttgg
     1321 tgctgaagaa gatattgaaa ttgtaggtac cgcaaacaac ggcaaaaaag catatgattt
     1381 atgcagaatc agaaaacccg atcttgttct catggacata caaatgccag aagtaaacgg
     1441 tgttgaagca acagcaatga taaaaaaaga ttttccgcaa acaaaaataa ttgtacttac
     1501 tacttttaat gacgatgaat atatttatga tgcattaaaa aacggtgcat cgggatatat
     1561 gttgaaagat gcttcaccaa aggaaatttt agaagccgta cgaactgttt ataatggggg
     1621 agcattgatt caatcagagg ttgcagttaa agtaatagac caattttcac agttggcaaa
     1681 agacacaggt gacaaacata ttgaccctaa tgtaggactt ttgacagaca gggaaattga
     1741 aatatgccgc cttatagcag aaggcaaaaa caataaggaa attgcggatg aattatatct
     1801 aagccaggga acagttaaaa attatataac aaaaatgtta ttaaaattag atttaagaga
     1861 ccgtacccaa ttagccattt tcacaataaa aaataatttg tagaagaatc tcttttttaa
     1921 aacctgagat ccttcgctta cgctcaggat gacaattaca gagcatattc gtttattgtg
     1981 gtgtggtaaa cacgtgataa ttctgagtgt attggtagaa tcttttttta aacagtgcca
     2041 aaagtcacat tattttttca agatttagtg atttaaggta ctgttattta actttttatt
     2101 ttaatattat gataccaatc aaagtattag gatacaaaga attggaggaa tagaaatgaa
     2161 aagaaaaata atttttgtaa cagtatttat acttatattg ctgattttgc ttacatcttg
     2221 caatacaaat aatttaattg aatataaaaa ggcatctgat aaaacagaac aaattattaa
     2281 aggccagaca gcaggagaat ttaccatgac gacggaaatc aacccggcag gattgacggc
     2341 ggaggaaata aaggagctta attatattaa ggacatgaat ggcagtttca gcgtagtatt
     2401 tgatgacgaa gaagaaaaga ctatcataag aaattatttg aattttggag gtttgggata
     2461 tgattttgaa gtctatatca atggggatga agtatccgta aagcttccgg ttgtcggtaa
     2521 atacctaaga attaacgagg aaatgctctc
//
LOCUS       DAOSHP010000143         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_41201_length_2477_cov_10.311726, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000143 DAOSHP010000000
VERSION     DAOSHP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41201_length_2477_cov_10.311726"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..244
                     /locus_tag="PLB33_09205"
                     /pseudo
     CDS             64..244
                     /locus_tag="PLB33_09205"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(328..903)
                     /locus_tag="PLB33_09210"
     CDS             complement(328..903)
                     /locus_tag="PLB33_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63745.1"
                     /translation="MDLRELSRKDFTSNNSLKSIPRICRRIFGLSEELRKHTLNKYEF
                     ELEKCLINEYVKSKIDSNISSKYNGNCNYGETLLNCYLDLIISFTTHKTEKEIEAYPD
                     FLVNPITGKNMEIDILFTGFKLGFEFQGEHHYNGRDIKAMDKDKEKLVLCVSQGTILI
                     PVNACQLNSNQLFNLISNSVKDYLNLGDISN"
     gene            1384..>2477
                     /gene="mef(A)"
                     /locus_tag="PLB33_09215"
     CDS             1384..>2477
                     /gene="mef(A)"
                     /locus_tag="PLB33_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(A)"
                     /protein_id="HOG63746.1"
                     /translation="MEKYINWKLKFYTIWAGQAVSLITSAILQMAIIFYLTEKTGSAM
                     VLSMASLVGFLPYAVFGPAIGVLVDRHDRKKIMIGADLIIAAAGAVLAIVALYTELSV
                     WMVMVVLFIRSIGTAFHSPALNAVTPLLVPEEQLTKCAGYSQSVQSISYIISPAAAAL
                     LYSVWKLNAIIAIDVLGAVIASITVAIVSIPKLGDQVQSLKPNFLREMKEGIVALRQN
                     KGLFALLLLGTLYTFVYMPINALFPLISMEYFNGTPVHISITEIAFASGMLVGGLLLG
                     RLGNFEKRVLLITGSFLIMGASLAVSGLLPPSGFVIFVACCAVMGLSVPFYSGVQTAL
                     FQEKIKPEYLGRVFSLTGSIMSLAMPIGLIL"
BASE COUNT          759 a          386 c          475 g          857 t
ORIGIN      
        1 tgcattttca gtatagtcta ttaaaaaact gtcggaaaaa taagggcaac ttacaaagct
       61 ttcagcttgg gtttggtggt ataataacga gagactgcat tcgtcacttg gctataaatc
      121 gcctgttgaa agccgtaaat gctgtgaggt tggagttact gacgaaaaaa ttgccaacaa
      181 gaaatatcaa aaattttgga agaaaatcac gttacataag caaaaggtag cgattgacaa
      241 ctaaaatcct atgcggtata atattattat cttacattgc attttcagaa tagtctattg
      301 aaaaactgtc gggaaaataa gggtaactta atttgatata tctccaaggt ttaaatagtc
      361 ttttacagaa ttggatatca aattaaagag ttgatttgaa ttcaactgac aagcatttac
      421 tggaatcaat attgttcctt gcgaaacgca aagaactagt ttctctttat ctttatccat
      481 tgcttttata tcacgaccgt tataatgatg ttctccttga aattcaaaac caagtttaaa
      541 tcctgtaaaa agaatatcta tctccatatt cttccctgta attggattta ccaaaaaatc
      601 ggggtaagct tcaatttctt tttcggtttt atgtgtagta aacgatatta tcaaatctaa
      661 ataacaattt aacaatgttt ctccataatt acagttgccg ttatattttg agctaatatt
      721 actatcaatt tttgatttta catattcatt tattaaacac ttttctaatt caaactcata
      781 cttattcaaa gtgtgttttc tgagttcttc ggataaacca aatattcttc tgcaaatcct
      841 tggaattgat ttgaggctat tgtttgatgt aaaatctttt ctgcttagct cccttaaatc
      901 catactcaga tgcttaaagc tgttctgaaa gttcaaatta tcgtttaaga agaccacttc
      961 gtcatagcct tcaaatcagg tttcgaaatg gaagtatgag atgcatttga ttatttttgt
     1021 attgaacaca tcgttttgtt gtgttattct ataggttgat ataaataaag atgtaggagg
     1081 aaccgaaact atgacagcat caatacgttt aagataagct ggcaataaaa aaggcagaat
     1141 ctatcccaat gataggcttt tttgttgtgc ttatttatac gatattgagc attcattagt
     1201 taaggtgagg atataggtta tttaactata cctttattta actatgtctt taatatgaat
     1261 gtttccagat tgtatgtatg cagaccaaaa gccacattgt ggattttggc ctgcattttt
     1321 tattgcctag aatactattt aaaaaagaaa tttaagcaaa ataatatgca ggagataata
     1381 gaaatggaaa aatatataaa ttggaaactt aagttttata ctatatgggc agggcaggcg
     1441 gtatctttaa tcactagtgc catcctgcaa atggcgataa ttttttacct tacagaaaaa
     1501 acaggatctg cgatggtctt gtctatggct tcactagtag ggtttttacc ctatgcggtc
     1561 tttggaccag ccattggtgt attagtggat cgtcatgata ggaagaagat aatgattggt
     1621 gctgatttaa ttatagcagc agctggagca gtgctcgcta ttgttgcatt gtatacggag
     1681 ttatctgtat ggatggttat ggtagtattg tttatccgta gcattggaac ggcttttcat
     1741 tctccggctc tcaatgcggt tacgccgctt ttagtaccag aagaacagct tacgaaatgt
     1801 gcaggttata gtcagtctgt gcagtcgata agctatatta ttagtccggc ggctgcagca
     1861 ttgttatact ccgtttggaa attaaatgca attattgcta tcgatgtatt gggcgctgtg
     1921 attgcatcta ttacggtagc aattgtaagt attcctaagc tgggcgatca agtgcaaagt
     1981 ttgaaaccaa atttcttaag agaaatgaaa gaaggaattg tcgcattgag acaaaacaaa
     2041 ggcttatttg ccttattact cttaggaacg ctatatacgt ttgtgtatat gccaattaat
     2101 gcactatttc ctttaattag catggaatac tttaatggaa cacctgtgca tatttccatt
     2161 accgaaatcg cttttgcatc tggaatgcta gtaggcggtc tactattagg aaggttgggg
     2221 aactttgaaa agcgtgtatt actaataaca ggttcattcc ttataatggg agccagttta
     2281 gccgtttcag gattacttcc tccaagtgga tttgttatat ttgtagcttg ctgtgcagta
     2341 atggggcttt cggtgccatt ttatagcggt gtgcaaacag ctctttttca ggagaaaatt
     2401 aagcctgaat atttaggacg tgtattttct ttgaccggaa gtatcatgtc acttgctatg
     2461 ccaattgggt taattct
//
LOCUS       DAOSHP010000144         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_42376_length_2429_cov_9.770008, whole genome shotgun sequence.
ACCESSION   DAOSHP010000144 DAOSHP010000000
VERSION     DAOSHP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42376_length_2429_cov_9.770008"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..624
                     /locus_tag="PLB33_09220"
     CDS             391..624
                     /locus_tag="PLB33_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOG63747.1"
                     /translation="MQKATIQLETLTCPSCMLKIEGAVKSIDGVDKDSLNVSFNTSKV
                     KLNFNDEKTSIKDIENAITKLGYEVKKSTVKPL"
     gene            714..1466
                     /locus_tag="PLB33_09225"
     CDS             714..1466
                     /locus_tag="PLB33_09225"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOG63748.1"
                     /translation="MTNIYKDDAIQEGIKALAGLMKESNYTVVLTGAGMDTESNIPDF
                     RGKDGLWRKIDPRLVASVDTLEENYSMFREFYIERINRLKGVNPHEGHYILAEFEEKG
                     IIKSIATQNVSALHELAGSKNVNELHGNIRSIRCNNCNKEANIDDFINNHKCEFCGSN
                     ILRPDVVLFGESLPAGPWNQAQSDIRKSDLLIVIGTSLEVSPVNQLPLLTRGKRALIN
                     NEDVYTYYDFDIKLIGKAKDILIRLRDTYNKI"
     gene            complement(1513..2424)
                     /locus_tag="PLB33_09230"
     CDS             complement(1513..2424)
                     /locus_tag="PLB33_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG63749.1"
                     /translation="MTMREFIQTHTGEIAALATAVCFTLNAISFESAGKKVGSLSVSY
                     IRLFIAFILLSISAYITRGLIMPTDATTSAWFWLLISGLFGFVLGDLFLFEAFVEIGS
                     RISLLIMSASPPITALAGFLFMREKLSIFALIGMTLNMLGISLVILSRGADEKKVEFN
                     RPVKGIIFACLGALGQAAGLIFSKIGMGSYNPLAATQIRIIAAVIGYTIIITWRNRWQ
                     EIKDSFKNKKALMQIAAGSVTGPYIGVVLSLVALQHTAAGIVSSITSISPIIIIPASI
                     VIFKEKILPKEILGAFISIAGVMLLFL"
BASE COUNT          861 a          415 c          461 g          692 t
ORIGIN      
        1 tgctccgata cggtgccaga aactcgtaga ggttcaggta tcaaaaacta gtaagtaaaa
       61 acaacatata attttgattt aaattaattc aaatagaaaa atattgtgta taatatatat
      121 aattctatcg tcatcctgag tagaatgaaa gatatcaggt cttgagattt ttcactacgt
      181 tcagaattat cacgtgttac caaaccacga taaacgaata tactctgtga ttgtcatcct
      241 gagcgcaagc gaaggatctc agggttaaaa aatgaggttc ttcgagctaa agctctcaga
      301 atgacacgta gcttattcat agcaatgaca attaaaaaac ggattaggaa tgccatataa
      361 aaataaataa attaaaaagg agagattaaa atgcaaaagg caactattca attagaaaca
      421 ttgacttgtc catcatgtat gctgaaaata gaaggtgcag taaaatctat agatggagta
      481 gataaagaca gcttaaacgt atcattcaac acaagcaagg taaaattaaa ttttaacgat
      541 gaaaaaactt caataaaaga tattgaaaat gcaatcacta aattgggata tgaagtaaaa
      601 aagtctactg taaaacctct gtaatacaac gtggtgcctt cccgtggtgc ctggcacccg
      661 aagcggtgcc tggcaccatt ggagtataaa tgtcaatgca aggagatgaa aaaatgacaa
      721 atatttataa ggatgatgca attcaagaag gtataaaggc tcttgccggc ttaatgaaag
      781 aatcaaatta tacagtagtg ctcacaggag ccgggatgga tacagagagc aatatacctg
      841 attttagagg aaaagacgga ttatggagga aaatagaccc aagattagtc gcaagcgtag
      901 atactcttga agagaattac tccatgtttc gtgagtttta tattgaaaga ataaacaggt
      961 taaaaggtgt aaatcctcat gaaggtcatt atatacttgc agaatttgaa gaaaaaggaa
     1021 taattaaaag tattgccact caaaatgttt cagctttgca tgaattagcc ggaagtaaaa
     1081 atgtaaatga gctgcatgga aacattaggt ctataaggtg caataattgt aataaggaag
     1141 caaatataga tgactttatt aataatcata aatgtgaatt ttgcggcagc aatattttaa
     1201 ggcctgatgt agtattgttt ggagaatcac tgcctgccgg tccttggaat caagcccaat
     1261 cagatattag aaaatcggat ttgctgatag tcataggaac aagcttagaa gtgtctccgg
     1321 taaatcagct accgctgctt acaagaggaa agcgtgcgct aattaataat gaagatgtat
     1381 atacttatta tgattttgac attaagctta ttggaaaggc taaagatata ttaataagac
     1441 taagggatac ttacaataaa atatgaaatc ggtgtcaggc accgtatgtg tgtgcctggc
     1501 acccctattt ggttacaaga ataacaacat tacccctgct atggaaatga atgctcccaa
     1561 tatttctttc ggtagaattt tctctttaaa aataacaatt gaagcaggga taataattat
     1621 aggcgatatg gatgtaatgg atgaaactat tcctgcagca gtatgctgca gtgccaccag
     1681 cgaaagaaca acaccaatat aaggtcctgt tacagagcct gcggctattt gcattaatgc
     1741 ttttttattt ttgaaggagt cttttatttc ctgccatctg tttctccagg ttattattat
     1801 tgtataaccg atgacagcgg caattattct tatttgcgta gccgccaaag gattatatga
     1861 acccatccct attttgctga atattaatcc tgcagcttgt ccaagagcac ccaaacaagc
     1921 aaatataatt cctttcacgg ggcggttgaa ttcaaccttc ttttcatcag ctcccctgct
     1981 aagtataaca agactaatac caagcatgtt taaagtcatt cctatcaagg caaaaattga
     2041 taatttttcc ctcataaaca gaaatcccgc cagtgccgta attggcggag atgctgacat
     2101 tataagcaat gatattcttg agcctatttc tacaaatgct tcaaataaaa ataaatcccc
     2161 tagtacaaat ccaaacaatc cggatatcaa tagccaaaac catgcgcttg ttgttgcgtc
     2221 ggtgggcata ataagtcctc ttgtaatata agcactgatg ctcagcaaaa tgaaagcaat
     2281 gaataacctt atgtagctta cagataagga gccaactttc ttacctgccg attcaaaaga
     2341 aattgcattc aatgtgaagc atactgctgt agccaatgcg gctatttctc ctgtgtgtgt
     2401 ttgtataaat tctctcatag tcattccct
//
LOCUS       DAOSHP010000145         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_42402_length_2428_cov_10.053940, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000145 DAOSHP010000000
VERSION     DAOSHP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42402_length_2428_cov_10.053940"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..599)
                     /locus_tag="PLB33_09235"
     CDS             complement(21..599)
                     /locus_tag="PLB33_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63750.1"
                     /translation="MNAIRKRIIIYISIICFLIAAWAQLFSLQGGNFNAQTCVITIIT
                     VAGGIIPIYLLFKDINNLKIARLIKENFILKVKTAVISEISEKEIPYKPINIEDTEIF
                     VSYFGILFNGKIAKFNQDGIRLTAMDIGNDYISFTYGNEKQMKNIRIVRPDIEPDAMS
                     EMINKFRFEMGITPTMVGKIITNKCNNEVKYG"
     gene            complement(798..2213)
                     /locus_tag="PLB33_09240"
     CDS             complement(798..2213)
                     /locus_tag="PLB33_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HOG63751.1"
                     /translation="MIKVKRNIPFIDLLNERNRAVLVHSLFSSWMLSLIFEGQIFQYL
                     AKEYNFEVGSIITASSAAMFLGLLAGGFIIKTKLHAKRLFLFSYPFFAALSLIFFFSP
                     SLLWAIGIIAGSLISGCCVAAWGFYFKTVSKKNERLKTAADVLIISNILMIILNSATI
                     NISTYVGLSLSIIILIFAFMIALKLPSDFNESYRYKKEESLSISTLLLFFYLFIVILT
                     INSGLMYHVVNPAFEHLTALISWYWAVPYVGAILILRNFSGKIKSSYILYVAIAMIGL
                     SFVAFITLDRSATSYFIINTLMLGSYGVYDLFFWSILGEMLNYHENPAKILGIGLSAN
                     VFGVALGGLIGNNLIANNLRSLSPSLLAFAIVCITLVLLPPLNKHLSSLLKGQTFLTE
                     FIEMPESKQKSEIDKLSTFGNLSEREKQVASLLLQGKTYKTIAAELFISENTVKYYVK
                     NIYSKFNIQSRAELIDIIVEK"
BASE COUNT          820 a          423 c          380 g          805 t
ORIGIN      
        1 ttccataacc aacaaatttt ttagccatat ttcacctcat tgttacattt atttgtaatt
       61 atcttaccga ccattgtggg tgtaatcccc atttcaaaac gaaatttatt aatcatttca
      121 ctcatggcat caggctcaat atcggggcgc acaatacgta tgtttttcat ttgtttttca
      181 ttgccgtaag taaatgatat ataatcattt cctatatcca ttgctgtaag ccgtatgccg
      241 tcttgattaa attttgcgat tttaccgtta aaaagaattc caaaatatga tacaaatatt
      301 tcagtatctt ctatgtttat tggtttataa ggaatttctt tttctgatat ttcgcttata
      361 actgcagttt tgactttcaa tatgaagttt tcctttatca gccttgctat tttcaaatta
      421 ttaatatcct taaataaaag gtatatcggt ataatccctc cggctactgt gattattgtt
      481 ataacacagg tttgagcatt aaagtttccg ccttgaaggg aaaaaagctg agcccatgca
      541 gctattaaaa agcatattat tgaaatgtag ataattattc tttttctgat tgcattcata
      601 atctccaccg catcttatat tgtttatccg ttacagacct tatattatgt ttatcatcct
      661 gagtgaaggc aagcctcatc ttcttaatga taaattgttt ttagtaatat tatagcagca
      721 aaaatgcaaa aaaaaatcac ccgaaagggg tagttttcaa aaaacagacc tacccttttc
      781 gggtaggtgg ttatttttca tttttctact attatatcaa tgagctctgc tcggctttga
      841 atattaaatt tagaataaat attctttacg taatatttta ctgtgttttc gctgatgaaa
      901 agctcagcag caattgtctt atatgtttta ccctgcaaaa gaagtgacgc cacctgtttt
      961 tctctttccg acaaattgcc gaatgttgaa agcttatcta tttcactttt ttgtttggat
     1021 tcaggcattt caataaattc agtaagaaag gtttgtcctt taagcaatga agaaaggtgc
     1081 ttatttagag gaggtaaaag caccaatgta atacaaacta tagcaaaagc aagcagggat
     1141 ggacttaaac ttcgtagatt atttgcaatt agattgttac cgataagtcc ccctaatgct
     1201 actccaaata cattggcaga aagacctatg cccagaattt ttgcagggtt ttcatggtaa
     1261 ttaagcatct cacctaatat gctccagaaa aacaagtcat agacaccata ggagccaagc
     1321 atcaacgtat taattataaa atagcttgtt gcagaccggt caagtgttat aaaagctaca
     1381 aacgaaagac caatcattgc aattgcaaca tatagtatat atgaagattt tattttccct
     1441 gaaaaatttc ttagaatcaa aattgcccca acataaggaa ctgcccagta ccagctaatg
     1501 agagctgtaa gatgctcaaa agccggattt accacatggt acatcaagcc tgaattaatt
     1561 gtcaatatta caataaacag ataaaaaaat aataagaggg ttgaaatact tagactttcc
     1621 tctttcttat acctgtagct ttcattaaaa tccgaaggaa gtttcagagc aatcataaat
     1681 gcaaatatca gtataataat ggacaatgac agtccgacat atgtggatat atttatagtg
     1741 gctgaattta gtattatcat taaaatgttt gaaattatga gaacatctgc tgctgttttt
     1801 aaacgttcgt tcttctttga aacagtttta aaataaaatc cccatgctgc aacacagcat
     1861 cccgaaatca aggagccggc aattattccg atagcccaca gtaatgaggg tgaaaagaaa
     1921 aatattaatg ataaagcagc aaaaaatgga taggaaaaaa ggaaaagccg ttttgcatgg
     1981 agttttgttt ttataataaa tccgccggcc agtaatccta aaaacatagc ggcagaacta
     2041 gcggtaatta ttgatcccac ttcaaaattg tactctttgg caagatattg aaatatttgc
     2101 ccttcaaata ttaatgacaa catccatgag gaaaacaatg aatgaaccaa taccgcccta
     2161 ttgcgctcat ttaacaaatc aataaaggga atatttcgtt taaccttaat catgatttag
     2221 cccgccctcc atgaatcatt tgcttatact taattatatt catattcata aactaaagtt
     2281 taaactgcat gataatatac attatactaa attataatac aaataacaat ctgctttttc
     2341 atattgacac ttttccctgc atatattata ctgtggttgg tggttgaacc acgataagga
     2401 gaggagtgat tatatggtta cgagtaac
//
LOCUS       DAOSHP010000146         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_44020_length_2369_cov_9.287813, whole genome shotgun sequence.
ACCESSION   DAOSHP010000146 DAOSHP010000000
VERSION     DAOSHP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44020_length_2369_cov_9.287813"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..196)
                     /locus_tag="PLB33_09245"
     CDS             complement(14..196)
                     /locus_tag="PLB33_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOG63752.1"
                     /translation="MEKNKKDKEKKQLLLRLSSSLWEELASWAEDDFRSINGQIEYLL
                     NECVKKRKKTSKKSAE"
     gene            complement(263..1264)
                     /locus_tag="PLB33_09250"
     CDS             complement(263..1264)
                     /locus_tag="PLB33_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOG63753.1"
                     /translation="MNKEKLITEEIVPSSMNGMFMLILNIVLMLGSILLFIISLSSLE
                     GALQVVMIVISILYFSVIGPVLFAGLKVLKPNEALVLTLFGKYYGTLKGEGFYFVNPF
                     VTSINPAGNAATSALLSIEGVSKDKKNPDLNINMKSKKISLKTMTLNNDRQKINDALG
                     NPITIGIVVIWRVVNTAKAVFNVDNYTEYLSIQCDSALRNIVRLYPYDTFGDDNEKTL
                     RGSSLEIANKLKEEIQEKVEVAGLEIIEARITHLAYAPEIAAAMLQRQQASAIIDARQ
                     MIVEGAVGMVEMALEKLSENNVVELDEERKAAMVSNLLVVLCGNKDAQPIVNSGSLY"
     gene            complement(1464..>2369)
                     /locus_tag="PLB33_09255"
     CDS             complement(1464..>2369)
                     /locus_tag="PLB33_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOG63754.1"
                     /translation="FLACLILIGTISALIYKLLPQIIENVNNIMSFIMEGDVDIAKIV
                     ADIKNRMDNKYIEYITDYILQAGESFKNIINNLLNYLYNMLMSLITNIGSSTFTVFIS
                     FIISIYMLSEKDDLLARSRRFIHAYFSDKKAKDILDVFEKANKIFKSFLGGKLLDSAI
                     VGIMFVIAFALAKVPYAPLMGTLTGFFNIIPYFGPLIGSVPVIIVSFFVNPTKALTAL
                     IIIIIIQQIDANILEPKIVGENVGVSPFWIITSVTVGGNLFGIPGLILGVPVVVLLKT
                     VLEGSMEMRLIEKDKEDIEKTNLRK"
BASE COUNT          682 a          476 c          341 g          870 t
ORIGIN      
        1 atacatttac tgcttattcg gcagattttt tgcttgtttt tttccttttc tttacacact
       61 cattgagcaa atattctatt tgaccgttta ttgagcgaaa atcatcctca gcccaacttg
      121 ctaattcctc ccaaagagat gatgataatc tcaataataa ttgtttcttt tctttgtcct
      181 ttttattttt ttctatgtcc gttttatttt tttctattat caccagctcc tttccatagt
      241 ttctcacact aatcacttac cgttaataaa gtgagcccga gtttactatg ggctgtgcat
      301 ctttgttacc gcaaagaaca actaacaaat tgctgaccat tgccgctttt ctttcttcat
      361 ctaattctac aacattattt tcactaagtt tttcaagtgc catttcaacc attccaacgg
      421 cgccttccac tatcatctgc cttgcatcga ttatagctga agcttgttgt ctttgaagca
      481 ttgctgcagc tatttcaggt gcatatgcca agtgagtgat tcttgcttca attatttcaa
      541 gacctgcaac ttctactttt tcctgaattt cctctttaag cttattggca atttccaaac
      601 tgctgcccct taatgttttt tcgttgtcgt cgccgaatgt atcgtaaggg taaagtctca
      661 caatatttct taaggcagaa tcacattgaa ttgacaaata ttcggtgtag ttatccacgt
      721 tgaaaactgc ctttgctgta ttaactactc tccaaataac cactattcct atagtaatgg
      781 gatttcctaa ggcatcattt attttttgcc tgtcattgtt taatgtcatt gtttttaatg
      841 atattttttt gcttttcatg tttatattca aatcaggatt ttttttatct ttactaactc
      901 cttcaattga aagtagtgct gatgttgcag cattgccggc cggatttatg gaagttacga
      961 acggattaac aaaatagaat ccttcacctt ttagcgtacc gtaatatttt ccgaataatg
     1021 tgagaacaag tgcttcattc ggttttaaca cctttagtcc tgcaaacaaa acaggaccta
     1081 tgacggaaaa atataaaatg cttattacaa tcataacaac ttgcagtgca ccttccaaag
     1141 aacttaaact aataataaac agcaatatcg agcctaacat taatacaata ttaagaatca
     1201 gcataaacat gccattcata gaacttggta ctatttcttc agtaatcaat ttttctttat
     1261 tcatttttga tatttcctcc ttatttgata tttcatgata tcaatttgat atcattatta
     1321 tattatacca tttcgctggc gtcaatatat tttgtagaaa ttatttatca taaattacta
     1381 aaaaaatatc gctacagctt tatgcgatat tttctgattt attctttatg acagtacccc
     1441 cgtccccttg tcacgtcccc ttgtcatttt cggaggttgg ttttttctat gtcttcctta
     1501 tccttttcta tcagacgcat ttccatggag ccctctaaga ctgtcttaag caggactaca
     1561 accggtaccc ctaatatcaa tcccggaatg ccaaaaagat ttcctccaac tgttacgctt
     1621 gtaataatcc aaaaagggct tactcccaca ttttctccta ctattttcgg ctccaatata
     1681 tttgcgtcta tctgctgtat gatgataata attatcagag cagtcaacgc tttggtagga
     1741 ttgacgaaaa atgatacaat aatcaccgga acagaaccta ttaagggacc gaagtaaggt
     1801 attatattaa aaaatcctgt cagagttccc atcaagggag cgtatggcac ctttgcaaga
     1861 gcaaatgcta ttacaaacat tattcccact atggcggaat ccagcaattt accgcctaaa
     1921 aaacttttaa atattttatt tgctttttca aagacatcaa gtatatcttt tgcttttttg
     1981 tcgctgaagt aggcgtgtat aaatctcctg cttcttgcca aaaggtcatc cttttcagac
     2041 aacatgtaga tactgattat aaagctgata aacacggtaa atgtactgga accgatgttt
     2101 gtaatcaatg acatcagcat attatagagg tagtttaaaa ggttatttat aatattttta
     2161 aaagattctc cggcctgcag aatgtaatcg gtaatatact caatatattt attatccatt
     2221 ctgtttttaa tgtctgccac tatctttgct atatcaacgt ccccttccat tataaacgac
     2281 attatgttat ttacgttttc tattatctgg ggtaatagtt tataaattaa ggcgcttatg
     2341 gttcctatta aaattaagca cgcgagaaa
//
LOCUS       DAOSHP010000147         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_46990_length_2265_cov_10.043891, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000147 DAOSHP010000000
VERSION     DAOSHP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46990_length_2265_cov_10.043891"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLB33_09260"
     CDS             <1..236
                     /locus_tag="PLB33_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63755.1"
                     /translation="SKTISSSFYFILFIRNRINNIPVIIDNMFTIYFIMLLSNKSYLS
                     KTEKPRIDIIKTNTTNTINIKAIFSTFFIFNMR"
     gene            complement(372..1112)
                     /locus_tag="PLB33_09265"
     CDS             complement(372..1112)
                     /locus_tag="PLB33_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOG63756.1"
                     /translation="MKKIKNKIIVISIILVFILGAGFYNYLSDYYKAEPSAVELMNKD
                     QNISIVDDENMITFKPGNSSSDIGLVFYPGGKVEHLAYIPLMQKIAERGYTCVLMKMP
                     FNLAVFDIDAADRAFKEIAEVDFWYVGGHSLGGAMSSEFAQKNPEKIKGIILLGAYTS
                     GDLSNTDLKLLSIYGSEDKILNLEALEESKTKEPKDSRLQIIKGGNHAYFGNYKEQKG
                     DGIAYITNDEQQNMTADMIAEFFEASNE"
     gene            complement(1164..1997)
                     /locus_tag="PLB33_09270"
     CDS             complement(1164..1997)
                     /locus_tag="PLB33_09270"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOG63757.1"
                     /translation="MEYKKSIINLIKERTSTRTYDGSGLEKEHLDDLNAYLDKVNQEI
                     KIKARFKIISNLGTGKKESKKLGTYGFIQGASTYIIGILDKDDNDALEFGYVFEKIVL
                     FATDLGVQTCWLGGTFSKDNFAELADLNENEFIAIISPVGYKKEKMRTFETTLRATIG
                     ADKRKPWNELFFNKSMKPLEEDEAGEFKMPLEMVRLGPSASNKQPWRIIKDEDMFHFF
                     ISRNKGYGITAYDLQKNDLGIAKCHFDLTLNELGIKGNWQELKDVLVPNDWEYAVSWT
                     L"
     gene            complement(2027..>2265)
                     /locus_tag="PLB33_09275"
     CDS             complement(2027..>2265)
                     /locus_tag="PLB33_09275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOG63758.1"
                     /translation="YFEVDEHTKTQLWKLVDECKEIVDKKFNPAGYNIGINCGEAAGQ
                     TVMHLRIHLIPRYLGDIENPRGGVRGVIPNKRIY"
BASE COUNT          697 a          482 c          265 g          821 t
ORIGIN      
        1 aatccaaaac aatttcttct tccttttatt tcattttatt tataagaaat cgtataaaca
       61 atattcctgt gataattgat aatatgttta caatatactt cataatgcta ttatctaata
      121 aatcatatct ctctaaaaca gaaaagccta gaatagatat aataaaaaca aatactacaa
      181 atacaataaa cattaaagca atttttagta ctttcttcat atttaacatg cgttagctcc
      241 ttaccttcat gttgcataaa tttaccgtta ttaatcccta atccatgaca ccattatatc
      301 agctgccgat gctccatatt catttgattt tcattattag acatatcata gcgcccatag
      361 acattttatc cttattcatt agacgcttca aaaaactcag ctatcatatc tgcagtcata
      421 ttctgctgtt catcatttgt aatataagca ataccatcgc ctttttgctc cttgtaattg
      481 ccaaaataag catggttgcc tcctttaata atttgaagtc ttgaatcctt tggttctttt
      541 gttttactct cttctaaagc ttcaaggttt aatattttat cctcagaacc ataaatagat
      601 agcaatttaa gatctgtatt gcttaaatct ccggaagtat aagcccctaa taaaataata
      661 ccttttatct tttcaggatt tttttgggca aactctgatg acatagcccc accaagagaa
      721 tgtccgccta cataccaaaa atctacttcc gcaatttcct taaaagctct gtcggctgcg
      781 tcaatatcga aaacagcaag attaaacggc atcttcatca gaacacatgt ataacctctt
      841 tcagcaatct tttgcattaa aggaatatac gccaaatgtt caacctttcc gccaggataa
      901 aatacaagac caatatcaga agaagaattc ccaggcttaa atgtaatcat attttcatca
      961 tcaacaatgc taatattttg atctttattc atcaattcga ctgctgaagg ttcagcttta
     1021 taataatctg ataaataatt ataaaaccca gcacctagga taaaaaccaa aattattgat
     1081 ataacaatta tcttattttt tatttttttc atataactct attcctattt ctatatcaag
     1141 tttattgaaa tattttttaa taattataaa gtccatgaaa ctgcatattc ccagtcattt
     1201 ggaactagga catcctttag ttcctgccaa ttgcctttta ttcctagttc atttagtgtt
     1261 aaatcaaaat gacatttagc aatacccagg tcattttttt gtaagtcata agctgttatg
     1321 ccatatccct tatttctgct aatgaagaaa tgaaacatat cctcatcctt aataattctc
     1381 cagggctgtt tatttgaagc agaaggacca agtctaacca tttcaagagg cattttaaat
     1441 tcaccggctt catcttcttc caaaggcttc atactcttat taaaaaacaa ctcattccag
     1501 ggctttcttt tatcagcacc gattgttgct ctcaatgtcg tttcaaaagt cctcattttt
     1561 tcctttttat atcctacagg cgatattata gctataaatt cattttcatt caaatctgcc
     1621 aattccgcaa aattatcctt gctgaatgtt cctcccagcc agcatgtctg aacacctaaa
     1681 tcagtggcaa ataaaactat tttttcaaat acatacccaa actctaaggc atcattgtca
     1741 tccttatcca aaataccaat aatataagtg ctggctccct gaataaatcc ataagttcct
     1801 aactttttgg attctttttt tcctgtccca agattggata taatcttaaa ccttgccttg
     1861 atttttatct cctggtttac tttgtccaaa tatgcattca ggtcgtctaa atgctccttt
     1921 tccagcccac tgccgtcata tgtcctggta gatgttcttt cttttattag attaattatc
     1981 gatttcttat attccatagt gatctcctat tctttgttta cataccctaa taaatgcgct
     2041 tattaggaat aactccccgc acccctcctc ttgggttttc aatgtcaccc agataccttg
     2101 gaattaaatg aatgcgcaag tgcatcactg tttgacctgc tgcttctccg caatttattc
     2161 ctatgttgta tcctgcaggg ttaaattttt tatccacaat ttccttgcat tcatctacta
     2221 atttccacaa ttgtgtcttt gtatgctcat caacctcgaa atagt
//
LOCUS       DAOSHP010000148         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_47481_length_2250_cov_11.547153, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000148 DAOSHP010000000
VERSION     DAOSHP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47481_length_2250_cov_11.547153"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1080)
                     /gene="ytvI"
                     /locus_tag="PLB33_09280"
     CDS             complement(7..1080)
                     /gene="ytvI"
                     /locus_tag="PLB33_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOG63759.1"
                     /translation="MTESEKKKFILDIAFIVTVYAVLYFIFVYVIHWVMPFIVGFLIA
                     LALRPVSRFVNRFVRSTGKGVALFVITMFYVVLAIIIWFLTSFFITQISGLIHTIPRL
                     YFNRIEPVLLEFNDWVVQNAETLSPNVASTISQIITNGINYLADFIKNISISFVQLAT
                     RLISNFPLYLISVIFTIVLSVFISLEYDNITAFFRRQMPEKFKDTFEEARKFITGTLW
                     KMIKSYILIMIITFIETFTGLSILKVNYALPIAAIIAVLDVMPVLGTGGVLIPWAIFE
                     FILKNYFLGFGLLALYIAVTVIRHIIEPRIVGHQIGLHPIITITAMYAGLRLFSVAGV
                     ILGPIVAITVKYLNDEGKIKLFK"
     gene            1387..2232
                     /locus_tag="PLB33_09285"
     CDS             1387..2232
                     /locus_tag="PLB33_09285"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOG63760.1"
                     /translation="MREINVEVITKTVEKLCIESNYYLPEDVKNALEDAMEKEESPLG
                     KEILSDILKNQKIARTKGVPICQDTGLAVIFLELGQEVRLVGGDLNEAINEGVRRGYL
                     NGYLRKSAVDDPFIERKNTGDNTPAIIHTKIVPGDKIKLIFAPKGGGSENMSALAMLK
                     PSDGIKGIKEFVLNTVEKAGANPCPPIIVGIGIGGTIEKTTLIAKQSLLRNVGEHNTN
                     PKVAKLEEELLEEINKLGIGPQGFGGRITALAVNIETFPAHIASMPVAINIQCHAARH
                     REAII"
BASE COUNT          830 a          356 c          405 g          659 t
ORIGIN      
        1 atctcatcat ttaaacaatt ttattttccc ttcatcattt aaatatttaa cagttatggc
       61 aacaatagga cctaatatta ctcctgccac tgaaaatagc cttaaacctg catacatagc
      121 ggttattgtt attataggat gaagtcctat ctggtgacct acaattcttg gctctataat
      181 atgtcttata acggtaactg caatgtataa agctaataat ccaaacccaa gaaaataatt
      241 ttttaaaata aattcaaaaa ttgcccaagg tatgagtaca ccacctgttc ctaaaaccgg
      301 cataacatca agaaccgcta taattgcagc aattggcagg gcataattaa cctttaatat
      361 agacaagccg gtaaatgttt caataaatgt aattatcatt attagtatgt aggattttat
      421 cattttccac aaggtaccgg ttatgaattt tcgtgcttct tcaaatgtat ctttaaattt
      481 ttcaggcatc tgtcttctga aaaaagctgt aatattatca tattccaagc tgataaatac
      541 cgaaagtaca atagtaaata ttacagatat tagatacagc gggaaatttg atattaatct
      601 tgtagcaagt tgtacaaaag aaatagatat attttttata aaatctgcaa gataattgat
      661 gccgtttgtt atgatttgtg aaatggtact tgcaacattt ggtgaaagtg tttctgcatt
      721 ttgaacaacc caatcgttaa actcaagaag caccggctca attctgttaa aatataatct
      781 tggtattgta tggataagtc ctgaaatttg ggtgatgaaa aaggatgtga ggaaccatat
      841 gattattgct aaaacaacgt aaaacattgt tattacaaat agagcaacgc ctttcccggt
      901 actcctgaca aacctgttta caaatcttga aacaggcctg agagccaaag ctattaaaaa
      961 gcccactata aaaggcataa cccaatgaat tacataaaca aagataaaat aaagaaccgc
     1021 atatactgta acaataaaag caatatctaa tataaatttt ttcttttcgc tttcagtcat
     1081 caacaagacc tcgatttttt ttaattggga acattcctaa aggcgtgtcc tatactatac
     1141 catgaaggcg tgtccctcat gaacgggtac ctcgtgtccc cgtactttat tttcaattat
     1201 aatattatta tttattattt gcaatagata agaaaattat tacatgaatt tacaaatgtt
     1261 agaaaatagt tccatatggt cgatgttata aaaattatta tttattatag acaagtttta
     1321 aaattattga tataaatata tgaagaggtt aattacggga cgtgaatatt atagaaggag
     1381 taatgcatga gagagattaa cgttgaagtc ataaccaaga cagtagaaaa actatgtata
     1441 gaatctaatt actatcttcc tgaggatgta aaaaatgctt tggaagatgc aatggaaaaa
     1501 gaagaatctc ctttgggtaa agaaatactg tctgacatac tgaaaaatca aaaaatagca
     1561 aggacaaaag gtgttcccat atgtcaggac acaggacttg cagttatttt tcttgagttg
     1621 ggacaggaag taagattggt gggcggagat ttaaatgaag caattaacga aggagtaaga
     1681 agaggttatt taaacggata tcttcgaaag tctgctgtcg atgacccctt tatcgaaaga
     1741 aaaaatacag gcgataatac tccagcaatt attcacacaa agatagtacc cggagataag
     1801 ataaaattaa tatttgcccc aaagggcggc gggagcgaaa atatgagcgc tcttgcaatg
     1861 ttaaaacctt ctgatggtat aaaaggcatc aaagaatttg tattgaacac ggtagaaaaa
     1921 gcaggcgcta atccatgccc tcccataata gtgggaatag gtattggcgg aacgatagaa
     1981 aaaacaacct tgattgcaaa acagtctctt cttagaaatg taggagagca taatacaaat
     2041 ccaaaggtag ctaaattaga agaagaattg ctggaagaaa ttaataagct gggtatagga
     2101 ccgcaaggct ttggaggaag aataacagca cttgccgtaa atatagaaac cttccctgct
     2161 catatagcaa gcatgcctgt tgcaataaat atacaatgtc acgctgcaag gcaccgggaa
     2221 gcaataattt aggaggttgt tatggcgaaa
//
LOCUS       DAOSHP010000149         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_48726_length_2211_cov_12.539889, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000149 DAOSHP010000000
VERSION     DAOSHP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48726_length_2211_cov_12.539889"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(793..1323)
                     /gene="raiA"
                     /locus_tag="PLB33_09290"
     CDS             complement(793..1323)
                     /gene="raiA"
                     /locus_tag="PLB33_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOG63761.1"
                     /translation="MKISIYSKNLQLTDSLREASIKKLRRLDKFFQQDIEAKVVLSIE
                     KKIHKVEVTIPFNGRIVRVEEYSDDMYNALDEAVESLEQQIRKYKTKLQDKRHSNESI
                     KFENIEPLEAEDDEEFKVVKTKRFAIKPMNIEEAILQMDLLKHNFFVFLNADTEEVNV
                     VYKRKDANYGLIEPEL"
     gene            complement(1522..2010)
                     /locus_tag="PLB33_09295"
     CDS             complement(1522..2010)
                     /locus_tag="PLB33_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOG63762.1"
                     /translation="MLYLDNRFMEVALPIIEHQEYQQMRYIKHHDESVFEHSVKVAFY
                     AYQIAYKRNLDWKATIRGALLHDFFLYKFKKCFSLRIVSDSIKHALAHPKIAFDNAVK
                     YFDLDEKEENIIKGHMFPFGVPKSKEAWIVSFVDKYIAVFEYSSNFKKMIQRKQPAYQ
                     AE"
BASE COUNT          717 a          360 c          331 g          803 t
ORIGIN      
        1 cctgcttttg tactatcttt gtataattct tctgcttcaa caatatattt tatagaccgg
       61 cttctaagct tgtcatgagc tctgaagtag gccgacaatg caccgaaaat tatggtcaat
      121 attggaatta caatatcctt gattatttct aacatattat cacatccttg gacattatat
      181 tcaataggag gaatttttgt tcttatgtgc agaaagtaag gcaattatga agaatgtgta
      241 taaaaattat ttgttttata aaattacaga gggatttggc tgttaattat tcataaaaat
      301 aaaaaattaa catcccggac tttttcataa agtgtttaac taattaaatt agattaaaaa
      361 ccgattaaat aaatttaaca atcagggaaa aaagaagata tgttctatat gttttcactt
      421 tatccacagc tgtggatgaa attgttaaca atttttaaat tcgaagactt taaggaaaat
      481 atttgtcaag tgtttttttt aagaaattca aaaatatttt tcattgaaat tccaacattt
      541 aatatatttt aaaattatac aaattatcaa cggctgtgga caactttatt tatacactta
      601 atttatcagc caggtataat tataagaata gttaaatgct aaatttatac aatattcgac
      661 aaaaattcaa gcatatttat gtaagcagtc agtgacacaa tttataagga tatgtaggta
      721 ttgcaggaag agataaaagc aaaaagacgc tcattgcgtc ttcctgcttt ttataattaa
      781 gaggtaaagt ttctaaagct ccggttcaat taaaccgtaa ttagcatctt tccttttata
      841 tactacattt acttcttctg tatctgcatt taggaaaaca aagaaattat gtttcaataa
      901 atccatttga agtattgctt cttctatatt catgggctta attgcaaagc gttttgtttt
      961 tactaccttg aattcttcat catcttcggc ttccaaaggc tctatatttt caaatttgat
     1021 tgattcattt gagtgtctct tatcctgcag ctttgtttta tatttcctaa tttgctgttc
     1081 cagggattca actgcttcat ccaaggcatt gtacatgtcg tctgagtatt cttcaacacg
     1141 tacgattctg ccgttgaagg gaatggtaac ctcaaccttg tgtattttct tttcaatact
     1201 taaaacaacc ttagcttcaa tatcctgttg aaaaaattta tccagcctcc ttaatttttt
     1261 tattgatgcc tctcttaacg aatcggtaag ttgcaaattt ttgctataga tagatatttt
     1321 catttagtca gcccctttca agtatttatt atatatattt aaatgttacc caattatgtg
     1381 aaaattaaac agtatattta caaataaaaa aaaagacaaa cagggtatgg ggacacacct
     1441 ccagtataaa gatagtctct gcgagcacga ggaatttccc caaataaaaa agacaaacat
     1501 ctgtttgtct ctcgctgcat cttattccgc ctgatatgcc ggctgtttcc tctgtatcat
     1561 tttcttaaaa tttgatgagt attcaaatac tgcaatatat ttgtccacaa agcttacaat
     1621 ccatgcttcc tttgattttg ggacgccgaa tggaaacata tgtcccttta taatattttc
     1681 ttctttttcg tccagatcaa aatatttaac cgcattatca aaggcaattt taggatgagc
     1741 caaggcatgt tttattgaat ctgaaactat tcttaaactg aaacattttt taaatttata
     1801 gaggaaaaag tcgtggagca atgctcctct tattgtcgcc ttccagtcta aatttctttt
     1861 atatgctatt tgataggcat agaaggcaac ttttactgaa tgttcaaaca cgctttcatc
     1921 atggtgctta atatatctca tctgttgata ttcctggtgc tcaatgattg gaagtgccac
     1981 ttccataaat ctattatcta aatataacat ctgaccgtcc tttaatagta gtagggggtg
     2041 ggttatttca taatactttc acatcttatt acgaaactgc ataaatagca agaagaattt
     2101 tttgaaaaag catgtatatt ttgtcattgt tatcaggggc agggggatac acctagagga
     2161 taagattcaa ccggctgtac cttcgaaatg acaaattaaa gaaatgtttt t
//
LOCUS       DAOSHP010000150         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_49213_length_2196_cov_10.230733, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000150 DAOSHP010000000
VERSION     DAOSHP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49213_length_2196_cov_10.230733"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLB33_09300"
     CDS             <1..329
                     /locus_tag="PLB33_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOG63763.1"
                     /translation="AGPTTSGRMDSYAPLLMEHGLKGMIGKGIRNQQVVDSIIKYNAV
                     YFAAIGGAGALLSEAIKEAEVIAFPDLGAEAIYKLRVENFPVTVIIDNKGNDLYKLGK
                     EKYKIV"
     gene            392..1831
                     /locus_tag="PLB33_09305"
     CDS             392..1831
                     /locus_tag="PLB33_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOG63764.1"
                     /translation="MNLKVVLKVLGTVLFWESVLMVPPLIISIIDNSYDRDAFIVTIV
                     LVGFIGIMLKSIKVTVNIRKREAYASVALSWLLMSLFGALPYYVSGTAPNYIDALFET
                     ASGFTTTGATIFTNVEILPRSILFWRSFTLWIGGMGVLVFTLALAPSLGARSIFLMRA
                     EFPGPSSGKLVPKLSDTAKILYIIYAIMTGVTIILFLIGGMPIFDAFIHAFGTAGTGG
                     LSLKASGLGYYGSSFIEWTTLVLMFLFGINFSIYYGALKGNWKTVKNEELIYYFILVI
                     SSVFLIFINILPVYNYSISDSIRNSAFHVTSIITTTGFATTDINMWPLLSKMVLLILM
                     VTGACSGSTTGGLKMIRVIILFKSVIRGINHTIHPSSVSKIKIGGKTVDDEVVDSTLI
                     FFFTYILILFVSATILSLDNFDFMTTFSAALTSISNLGSGFGLIGPLGNFAGFSNLSK
                     FTMTLCMIIGRLEVLPVIALLSPTMWKKQ"
     gene            1919..>2196
                     /locus_tag="PLB33_09310"
     CDS             1919..>2196
                     /locus_tag="PLB33_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63765.1"
                     /translation="MNRYPYMDFLPIYPEYILAQQQEDTRQIMQTIRSEHSDVYSELQ
                     RMGLNRNLADLLITFIVNFTIRQERNLQPRQIYDRFRNQTPWFNLILR"
BASE COUNT          700 a          347 c          448 g          701 t
ORIGIN      
        1 cagcaggacc tacaaccagc ggaagaatgg attcatatgc acctttatta atggagcacg
       61 gtcttaaggg tatgataggc aagggtatca ggaaccaaca agtggttgat tcgataatca
      121 agtacaatgc agtatatttt gcggctatcg gaggtgccgg agcactgctt tcagaagcaa
      181 taaaagaagc agaagtaatt gcattccccg atttgggagc agaagcaata tataaattga
      241 gagtggaaaa ttttcctgtt accgtgataa ttgacaataa aggaaatgac ttatataaac
      301 tagggaaaga aaagtacaaa atagtttaat aaagatacag ttgttcaggc aaagagaccg
      361 gaacagaaca aaaaaacttt ccggaggaaa aatgaattta aaggttgttc ttaaagtact
      421 tggaactgtt ttgttttggg aatcggtatt aatggttccg cctcttatta tttcaataat
      481 agataacagt tatgacagag atgcatttat tgttacaatt gttttagttg gctttatagg
      541 aattatgctg aaaagtatta aagttaccgt gaatatcaga aaacgtgaag cttatgcttc
      601 agtagcgctg agctggcttt tgatgtcttt atttggagct ctgccatact acgtcagcgg
      661 tactgcaccc aattatatag atgctttgtt tgaaactgct tccgggttta cgactacagg
      721 agcaactata tttacaaatg tagaaattct tcctcgaagc atattgtttt ggaggagttt
      781 taccctttgg attggaggaa tgggagtatt ggtgttcacc ttggcgttag caccttcact
      841 gggggcacgc tcaatatttt tgatgcgggc tgaatttccg ggaccctctt ccggaaaatt
      901 agttccaaag ctttcagata cagcaaaaat attgtatatt atatatgcga taatgacggg
      961 agttacaata attttatttt tgataggcgg catgcccata tttgatgcgt ttattcatgc
     1021 attcggaact gcaggtacag gcggtttatc actgaaagca agcggcttag gatattacgg
     1081 cagttcgttt atagaatgga caaccctagt gttaatgttt ttattcggaa ttaatttttc
     1141 tatttactat ggagcgttga agggcaactg gaaaactgtc aaaaatgagg aattgatata
     1201 ttattttata ttagttattt catctgtatt cttaatattt ataaatatat tacctgttta
     1261 taattattct atatctgatt ccataaggaa ctcagccttc cacgtaactt caataattac
     1321 tactaccggt tttgcaacaa ctgacataaa catgtggccc ttgttgtcca agatggtctt
     1381 gctgatattg atggttaccg gtgcctgctc cggctcaaca acaggcggtt taaaaatgat
     1441 acgtgtaata atactgttta aatcagttat aagaggaatt aatcatacca tacatccaag
     1501 ttctgtcagc aagataaaaa taggcggtaa gacagttgat gatgaagtag tagatagtac
     1561 actgattttt ttctttacct atatattaat tttatttgtt tcggctacga tattgtcatt
     1621 ggataatttt gactttatga caacattttc ggctgccttg acatccataa gcaacttagg
     1681 ctcaggattt ggtctgattg gacctttggg aaactttgca ggattttcaa acttaagcaa
     1741 gtttacaatg accttgtgca tgataatagg aagattggaa gtgctgccgg taatagcatt
     1801 attatctcca actatgtgga agaagcaata attattgtta ataattacct ccgttaaggg
     1861 aacaaatctt gctctgttaa ataaaataga gtgagattat ttatttaggg aggttattat
     1921 gaacagatat ccatatatgg attttcttcc aatatatccg gaatatattt tagctcagca
     1981 gcaagaagat actcgtcaaa ttatgcaaac catccgctca gaacacagtg atgtatactc
     2041 agaactgcag aggatggggt taaacagaaa tcttgctgat ttactgataa cttttattgt
     2101 taacttcaca ataaggcaag agagaaatct acagccgaga cagatatatg acagattcag
     2161 aaatcaaact ccatggttta acttaatatt aaggca
//
LOCUS       DAOSHP010000151         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_49518_length_2187_cov_11.294559, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000151 DAOSHP010000000
VERSION     DAOSHP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49518_length_2187_cov_11.294559"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..648
                     /gene="greA"
                     /locus_tag="PLB33_09315"
     CDS             178..648
                     /gene="greA"
                     /locus_tag="PLB33_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOG63766.1"
                     /translation="MKGNETLITIEGLEELKRELEELKIVKRKEISEKIKAALAFGDI
                     SENAEYDEAKNEQAIVEARIAKLETMIKNAKVIKTTKKNGVVCIGSKVRIKDLEFDEE
                     MEYTIVGSAEADPFKGRISNVSPLGKALLGKKLGDIIEVASPAGDMIKYEILTV"
     gene            681..2153
                     /gene="lysS"
                     /locus_tag="PLB33_09320"
     CDS             681..2153
                     /gene="lysS"
                     /locus_tag="PLB33_09320"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOG63767.1"
                     /translation="MDEQQISDLRQIRISKLKDLINAGRDPYKVSKYEVTSNSKQIKD
                     NFEEMEGKFVSIAGRIMSKRGQGKVGFYDIQDHLGRIQMFVKLDNIGEEEYNWLKTYD
                     IGDIIGVKGEVFKTKTGEVSVRAEEIKLLSKSLQTLPEKFHGLKDMDLRYRQRYVDLI
                     VNPEIKEVFLMRNKIIRGIREYLDGEGYLEVETPILSPIAGGANARPFITHHNTLDID
                     MYMRIANELFLKRLVVGGLGDGVYEMGKMFRNEGMDANHNPEYTAMEVYKPYADFNDM
                     MTLTENIVAYVAKKARGTTKIEYQGKMIDFTPPWRRVKMQDMVKEYAGVDFDLINTDK
                     EAIEIAKEKGIEITPLMTRGHVISELFEKYCEEHLEQPTFVTHHPVEISPLAKRNAED
                     PRLTDRFEAFANTWEIANAFSELNDPIDQRERFEAQVRQKEAGDEEAHMMDTDFINAI
                     EVGLPPTGGLGIGVDRVIMLITNQPTIRDIILFPTMKPLN"
BASE COUNT          878 a          299 c          456 g          554 t
ORIGIN      
        1 accttaacgg tttaaattaa caaattaact tgacaataat cgtttacttg tttataatgt
       61 tctaattaat tgaaagattt gtcatcaaat tatcataata tattaaaatg ataataaaat
      121 gaaatatata ttgatgacat gtaaatataa ggtaacatta ttaaaaggag caatattatg
      181 aaaggtaatg aaactttgat tacaattgag ggtttggaag aattaaaaag ggaattagaa
      241 gaattaaaaa tagtaaaaag aaaagaaata tccgaaaaaa ttaaggctgc tttagctttc
      301 ggggatataa gcgaaaatgc cgaatatgac gaagccaaga atgaacaagc aatcgttgaa
      361 gcgagaattg caaagcttga gacaatgata aaaaatgcga aggtgataaa aacaacaaag
      421 aaaaacggag ttgtatgcat aggctcaaaa gttagaataa aggatttgga atttgacgag
      481 gaaatggaat atacaatcgt aggttccgct gaagcagatc cgtttaaagg aagaatatct
      541 aatgtttcac ctttgggaaa agctttattg ggaaagaaac tcggtgatat aatagaagtt
      601 gcatcccctg ccggagatat gataaaatat gaaatactaa ccgtttaaat aaaacaacta
      661 atgatgaggt ggtgttaaaa ttggatgagc agcaaataag cgacttaaga caaataagga
      721 ttagcaaatt aaaagattta attaatgcag gaagagaccc ctacaaagtt tcaaaatacg
      781 aagtaacctc taattcgaaa caaatcaagg ataattttga agaaatggaa ggaaaattcg
      841 tatcaattgc cggaagaata atgtctaaga gaggacaggg aaaggttgga ttctacgata
      901 tacaggacca cttgggaaga atccagatgt ttgttaaact ggacaacata ggcgaggaag
      961 aatacaattg gctgaaaact tacgatattg gcgatataat aggcgttaag ggtgaagtat
     1021 tcaaaacgaa aacaggagaa gtttccgtaa gagccgaaga aataaaactt ttatccaaat
     1081 ccttgcaaac acttcctgaa aagttccacg gattaaagga tatggattta agatatagac
     1141 aaagatatgt ggatttaatt gttaaccccg aaatcaaaga agttttttta atgcgtaata
     1201 aaataataag agggattaga gaatatttag acggagaagg atatctagag gttgaaactc
     1261 ctattttaag tccaatagca ggcggagcaa atgcaagacc attcattact catcataata
     1321 cattggatat tgacatgtat atgcgcattg ctaatgagtt atttttaaag agacttgtgg
     1381 ttggagggct tggagacggc gtttacgaaa tgggaaaaat gtttagaaat gaaggaatgg
     1441 atgcaaacca caaccctgag tataccgcca tggaagtata taaaccatat gcagacttca
     1501 atgatatgat gactttaaca gaaaatatag ttgcatatgt tgcaaagaaa gcaagaggta
     1561 caactaaaat agaataccag ggcaaaatga tagactttac tccgccttgg agaagagtta
     1621 aaatgcagga tatggttaag gaatatgcag gagttgattt cgacctgatt aataccgata
     1681 aagaagcaat tgaaattgca aaagagaaag gaattgaaat cacgcctctt atgacaaggg
     1741 gtcatgtaat aagcgaattg tttgaaaagt attgtgaaga gcatttggaa cagccaacct
     1801 ttgtaacaca tcatcctgtt gaaatatcac ctttggctaa gagaaatgca gaagacccgc
     1861 gattaacaga tagatttgaa gcatttgcaa acacatggga aattgcaaac gcattttcgg
     1921 aattaaatga ccctattgac caaagagaaa gatttgaagc acaagttagg caaaaagaag
     1981 ccggtgatga agaagcccat atgatggata cagacttcat taatgcaatt gaagtaggtc
     2041 tccccccaac aggcggactc ggcataggag ttgacagagt aattatgtta ataacaaacc
     2101 agcccacaat aagagacatc atattgttcc caacgatgaa accattgaat taattataaa
     2161 aaaatggcaa taattgaacc gacctcc
//
LOCUS       DAOSHP010000152         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_50247_length_2165_cov_9.448815, whole genome shotgun sequence.
ACCESSION   DAOSHP010000152 DAOSHP010000000
VERSION     DAOSHP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50247_length_2165_cov_9.448815"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1137)
                     /locus_tag="PLB33_09325"
     CDS             complement(199..1137)
                     /locus_tag="PLB33_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOG63768.1"
                     /translation="MKTIITEEMRYRQRVVEYAIKHDNNARAARRYHTSRQQVQRWRK
                     KYDGTVESLANRSRRPKFHPNQHTQEELNLIRHKHRYHGQEGYAQVYRKLRDVGYRRS
                     YDSMCRQIRKMKLNVARTRDSYPKSSYHKEAVTYPGERVQIDVKYVPNECIGFASHHS
                     RYYQITAIDEYSRKRYMELVDENSTYTTSRFLERLEYNLGFKVKLAQTDNGFEFVNDT
                     ERTNKKTKFQKTLEKLNIKHKRTRPYSPWQNGIVERSHRIDNDLFYNKRRFNSYNQML
                     KSFNRYKNRYNNIARKILGFKTPNEIVEEYFSKNAA"
     gene            complement(1248..2039)
                     /locus_tag="PLB33_09330"
     CDS             complement(1248..2039)
                     /locus_tag="PLB33_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOG63769.1"
                     /translation="MDKITIVEFATEFLLNDLNHVSRELAIRDELAGMKIFNEPIFAY
                     GDADDNGFQSLKETTVVGEHFMLPKEWLPEAKTVIAYFFPFTDEIIESNRMDMLWPSN
                     EWLHGRIEGQNFLLEFSRFLYSKLNDAGYKSLVPGLDHRFKTKGFTSNWSERHVSYVC
                     GLGTFGLSKGIITEKGMAGRLGSIITELKLEPGIKKYKDVYEYCTLCGNCAINCPVDA
                     ISVEEGKDHRKCSDFLDTVREKHKTRYGCGKCQVSVPCEKGIPRG"
BASE COUNT          594 a          425 c          295 g          851 t
ORIGIN      
        1 ataacttatt tatgaaatat gtcaataact cagaattacc ctcttggtat tccctctaga
       61 gaagtcaaac atatgtgaca cagacctctg aaaattttct acgaataatg ctgtggtttt
      121 ttttaattgc taataattat tttgtcaagg aggaaagcga tagcgatcct tgattaaata
      181 attattagta ataccatctt aagcagcatt tttagagaaa tactcctcaa ctatttcatt
      241 tggagtttta aaaccaagga tttttcttgc tatattatta tatctgtttt tatatctatt
      301 aaatgacttt aacatttgat tatagctgtt aaatcgtctt ttattataaa ataaatcatt
      361 atctattcta tggctacgct ccactatgcc gttttgccat ggcgaataag gcctagtcct
      421 tttatgcttt atattaagct tttctaaggt cttctgaaac tttgtcttct tatttgttcg
      481 ttcagtatca tttacaaatt caaatccatt atctgtctga gctaatttta ctttaaatcc
      541 taaattatac tctaatcgct ctaaaaacct tgaagttgta tatgtactat tctcatctac
      601 cagttccata tatctttttc ttgaatattc atcaatagct gttatttgat aatatcgact
      661 atgatggctc gcaaatccaa tacattcatt cggaacatat tttacatcta tttgaactct
      721 ttctcccgga taagtaacag cctctttatg atagcttgat ttaggataac tatctcttgt
      781 ccttgctaca tttagcttca tttttcttat ctgcctacac atagagtcat agcttctcct
      841 ataaccaaca tctcttaatt tgcgataaac ttgcgcgtat ccttcttgcc catgatatcg
      901 gtgcttatgc cttattaagt ttagttcttc ttgtgtatgt tggttaggat gaaacttagg
      961 tcttctgctt ctgtttgcta aagattctac tgtaccgtca tattttttcc tccagcgttg
     1021 aacctgctgg cggcttgtat gatatcttct tgctgctcta gcgttgttat catgttttat
     1081 tgcatattct actactcgtt gacgatatct catctcttct gttataattg tcttcatagg
     1141 aaaggttccc tccgattttt gtttgttgta gtaaactaca ttatatcaga ggcctttcct
     1201 ttttttatgt tttattgtca catatgtatt atctcatatc atcagaatta ccctcttggt
     1261 attccctttt cacagggaac agatacctga cattttccgc agccgtaacg tgttttatgt
     1321 ttttctctga ctgtatccaa aaaatcagaa catttcctgt ggtcttttcc ctcttcaaca
     1381 gatattgcgt caacagggca attaatagca caattaccac acaatgtgca atattcatac
     1441 acatccttat atttttttat gcctggttct aattttaatt ctgttataat acttcccaat
     1501 cttcctgcca ttcctttttc tgtaattatt ccttttgaca agccaaaggt tccaaggccg
     1561 catacataag atacatgcct ctctgaccag ttgctggtga aaccctttgt tttgaatcta
     1621 tggtctaatc ccgggacaag gcttttataa cctgcatcat ttaatttgga atataagaat
     1681 ctgctgaatt ctaataaaaa attctgacct tcaatacgtc cgtgcagcca ttcatttgac
     1741 ggccagagca tatccattct gttgctttct attatttcgt ctgtaaatgg aaagaaataa
     1801 gcaataactg tttttgcttc tggaagccat tcctttggca gcataaaatg ctcacctaca
     1861 acagtggtct ctttcaatga ttggaatcca ttgtcatctg catccccata agcaaatatc
     1921 ggctcgttaa atattttcat tcctgcaagc tcatctctta ttgctaattc tcttgataca
     1981 tgattgaggt catttagtaa aaattcagtt gcaaattcta cgattgttat tttgtccata
     2041 ttttcacccc taaatttatt aaaattattt tttattaaac ttatttgact tattaaagca
     2101 taagatatac ccttattata caatatttcc ctgattaagt gttattgaaa ttcaaataaa
     2161 gttat
//
LOCUS       DAOSHP010000153         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_54129_length_2060_cov_8.774065, whole genome shotgun sequence.
ACCESSION   DAOSHP010000153 DAOSHP010000000
VERSION     DAOSHP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54129_length_2060_cov_8.774065"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLB33_09335"
     CDS             <1..197
                     /locus_tag="PLB33_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOG63770.1"
                     /translation="AAKTKKGKWIFGIIIGVVTVLIRALSLFNGGMMFAILMGNTFAP
                     IIDHFIKEAEKRKKSKAVAA"
     gene            194..787
                     /locus_tag="PLB33_09340"
     CDS             194..787
                     /locus_tag="PLB33_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOG63771.1"
                     /translation="MKNSFIYPIIFMMAVTAIFISVLAGLNFVTADTISFNQESQLQQ
                     KILYIFDILPEGGTEKDIERVFNDSVIEKEWGELRGYALIQGGKETAYAIPVNGAGLW
                     GSIIGYIGLNADYTEIIGIEFVTQNETPGLGGRISEDSYKEQFRGIDISGKTENFIIS
                     SPEPGSNVDAIAGATQTSNAVVKLINDDIKIFLREVE"
     gene            789..1412
                     /locus_tag="PLB33_09345"
     CDS             789..1412
                     /locus_tag="PLB33_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOG63772.1"
                     /translation="MTKTEVKKIFKKGIYTDNPIFVQVIGICSALAVTNLMINSLIMG
                     IALALVTAFSSLTVSLLKDHTPKHIRMMVQVLIISAYVIMVDIYLKAYMPEISKALGP
                     YVGLIITNCIIMGRAEGFARSNPPIPSFFDGLASGIGYTFVLMSISFIREILGFGSIF
                     GISIIPETFTRWTLMVMPPSAFFILALIMWVFNNNRLKKQREGVERK"
     gene            1409..1999
                     /locus_tag="PLB33_09350"
     CDS             1409..1999
                     /locus_tag="PLB33_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOG63773.1"
                     /translation="MIQVHPFIIFIASIFTSNMIFSNFLGMCSFIAVSKEIPTALGLG
                     IAVTMVLSATTVLNYLVYWYIIMPLGLEYLMYILFILVIAAFVQLLEMTMDRYLPTLY
                     YALGIFLPLITVNCAILGVSLFMVTRQYNFFQTFGFAVGSGIGWALAITAMAGIRSKL
                     NENSIPEGLRGVPITLIITGIMALAFIGFSGMVQIQ"
BASE COUNT          628 a          315 c          440 g          677 t
ORIGIN      
        1 gtgccgccaa aacaaaaaag ggcaagtgga ttttcggaat aataataggt gttgtaaccg
       61 ttctgattcg ggctctgtcg ctttttaacg gcggtatgat gtttgcaata ttaatgggta
      121 atacatttgc acctataatt gatcatttta ttaaggaagc tgaaaagaga aagaaaagta
      181 aggcggtggc agcatgaaaa attcttttat ttatccaata atatttatga tggcagttac
      241 cgcaatattt atttcagtct tggcaggttt gaatttcgtt accgcagata caatttcctt
      301 taatcaagaa agccagctgc agcaaaaaat tctttatatt tttgatatat tgccggaagg
      361 cgggacggag aaggatatag aaagagtatt taatgatagt gtaattgaaa aagagtgggg
      421 agagctaaga ggttatgcat tgattcaagg cggtaaagaa acagcatatg caattcctgt
      481 taacggtgca ggactttggg gctccataat aggatatatt ggattaaatg cagattacac
      541 agaaattata ggtattgagt ttgtaacaca aaatgaaacc ccgggacttg gcggcagaat
      601 atcagaggat tcatacaagg agcagtttag gggtatagat atatccggta agacagaaaa
      661 ttttattatt tcatctcctg agcctggcag caatgttgat gctattgccg gtgcaaccca
      721 aacgtccaat gccgttgtaa agctcataaa tgatgatata aagatatttt tacgggaggt
      781 ggaataaatt gaccaagaca gaagtaaaaa agatatttaa aaagggaata tacaccgaca
      841 atccgatatt tgtgcaggta ataggaattt gctctgcatt agctgtaact aatttaatga
      901 taaattcgct gattatggga attgctcttg ctctggtaac tgcattttct tcattgacgg
      961 tatctctgct taaagaccac actcccaaac atattcgtat gatggtacag gtattaatta
     1021 tttcagcata tgtaattatg gttgatattt atttgaaagc ttacatgcct gaaataagta
     1081 aagcattagg accttatgta ggacttataa ttacaaattg tatcataatg ggaagggctg
     1141 aaggatttgc ccggtccaat ccgccaatac cgtcattttt tgacggtctt gcatccggta
     1201 taggttatac ctttgtttta atgtctattt ccttcataag agaaatttta ggttttggaa
     1261 gtatttttgg aatcagtatt attcctgaaa cttttacaag gtggacatta atggttatgc
     1321 ctccttcggc atttttcata cttgcattaa taatgtgggt ttttaacaac aatcgtctaa
     1381 aaaaacagag agaaggagtt gagagaaaat gattcaggtc catcctttta taatatttat
     1441 tgcttctatt tttacaagca acatgatttt ttcaaatttt ttggggatgt gctcattcat
     1501 tgccgtttca aaggaaatac caactgcctt gggcttggga attgctgtta caatggtgct
     1561 ttcggctaca accgtgttaa attatcttgt atattggtac attataatgc ccttaggatt
     1621 ggaatactta atgtatatat tgttcatttt ggttattgca gcatttgtac agcttttgga
     1681 aatgacaatg gacagatatt taccgactct atactatgcc ttgggaattt ttctgcctct
     1741 tataaccgtt aattgtgcca tacttggagt ttcattgttc atggttacca ggcagtataa
     1801 ttttttccag acttttggtt ttgcagtagg ctcaggtatc ggttgggctc ttgccataac
     1861 tgcgatggcg ggaataaggt caaagcttaa tgaaaactcc ataccggagg ggttaagggg
     1921 agttccgatt actttaataa ttaccggaat tatggcctta gcattcatcg gtttcagcgg
     1981 tatggttcaa atacagtagg aggcgtagga ggcattatgg atacaataat tatgacaatt
     2041 gtaatattct ccggaatttc
//
LOCUS       DAOSHP010000154         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_54326_length_2055_cov_9.232500, whole genome shotgun sequence.
ACCESSION   DAOSHP010000154 DAOSHP010000000
VERSION     DAOSHP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54326_length_2055_cov_9.232500"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            509..751
                     /locus_tag="PLB33_09355"
     CDS             509..751
                     /locus_tag="PLB33_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63774.1"
                     /translation="MKTKKGSILDELKFNQLSRESESKIISGKSIWDYNWSTCTPTGK
                     LYHYIDDAGISHLVLDERITDLNEIIACGDNWAAPC"
     gene            843..1694
                     /locus_tag="PLB33_09360"
     CDS             843..1694
                     /locus_tag="PLB33_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63775.1"
                     /translation="MNTIFHNFKYITVISIVVTMHFSCTNRINLNKISGEWLLDTDRK
                     SKYIFRKDGTYDYKFKCYSIYENYGTPRYKFYSKNDTLWLDFKYKYPQIDGKIVTQRD
                     KAAIQINGDYLTKVTFKKGAGINNHIDEYSTWIKDITSPRRILDCKNNMTFVIPTNFY
                     GNLWIAFNQPSGEIVEYDSIGREILRIPESGKHLTQLPENAFAVANKCYNIISTDSLS
                     MNYNQIPFIDINEMIDSTFINNYERYAIVYGFNQYPRERINQIFGKSITGNVLKIYIG
                     SNNISKK"
BASE COUNT          708 a          304 c          376 g          667 t
ORIGIN      
        1 caaatggtaa agttgtttac cagaacagcg gctatgagca gcttttcacg gtgccattag
       61 ctaattcctt caaggcatga actattctga aattaagcgc tttaccgatt atctggatta
      121 cctgttgcgg tgcggcaatg ccggaaccgc agccgagatt gccgacaggc tcggcacttg
      181 tgagcgcacc gtacgttcct acttcgagca gctaaagagc atgggcgatc ccctggagaa
      241 cgattgcgtt cgcagaacct ggcgctacac ccgttccgga aggctcactc tggggtttgt
      301 tgacgaagat tgcggtgctg catgcaatgc cgatgcttct acttcccccc cctaaaaaca
      361 tgttgtagaa cataaaaaaa attacaggcc ggaaaggtag ctgccgacct ggtcttttac
      421 tttgcagtaa ccaatccatt aaaataagca gcgctggatt gtttttgtag tttgaaggag
      481 tttattgttc aactaaaact aaagagacat gaagactaaa aaaggatcta ttttagacga
      541 attgaaattt aatcaattat caagagagag tgagagcaaa ataataagtg ggaaaagtat
      601 ttgggattat aattggtcaa cttgtacgcc aacgggaaaa ctatatcatt atattgacga
      661 tgctggaatt tctcatttag tacttgatga gcgaataact gatttgaatg aaataattgc
      721 atgtggagac aattgggctg ccccttgtta atgttaaaaa tagattttac ttttacatta
      781 ataaatagac gggtaagtat ttaatactat cccgtttgtt ttttaaataa agataatgga
      841 agatgaatac aatatttcat aattttaagt acataactgt aatttcaata gttgttacaa
      901 tgcatttctc ttgtacaaat agaattaatt taaataaaat atctggagaa tggttattgg
      961 atacggatag aaaatccaaa tacatattta ggaaggatgg gacttatgat tacaaattta
     1021 aatgttattc aatttatgaa aattatggga ctccaagata taagttttat tctaaaaatg
     1081 atactttgtg gttagatttt aaatataaat accctcaaat tgatgggaaa attgttacgc
     1141 aaagagataa ggcagctatt caaattaatg gggattacct tactaaggtt acttttaaaa
     1201 aaggggcagg tattaataat catattgatg aatatagtac atggataaaa gatattacaa
     1261 gtcctagaag gattctggat tgtaaaaata atatgacgtt tgtaattcca acaaatttct
     1321 atggtaattt atggattgca tttaatcaac catcaggcga aattgtagaa tatgatagta
     1381 ttggcagaga aatattaaga atacccgaaa gtggtaaaca ccttacacag ttacccgaaa
     1441 atgcttttgc agttgcaaat aagtgttata acatcataag cactgatagt ttatcaatga
     1501 attataatca aattcctttt attgatataa atgaaatgat tgattcaaca tttataaata
     1561 attatgagag atatgctatt gtttatggat ttaatcaata tcctcgtgag cgaataaatc
     1621 aaatatttgg aaaaagtatt actggtaatg tcctaaaaat atatataggt tcaaataaca
     1681 tttcaaaaaa gtaaattatt tgggtgttgt tgttttaatt ttctcaagat gagtgtttta
     1741 attattacgg ataaagaaga tcattcattt actgatgtaa ttgaatggct taagaataag
     1801 aatgtcaatt atttgattac ctaaaatccg caagtgaaaa aattggtttg aaaaatttca
     1861 tgttttaggt ctatttcaag gaatttaatt cattttttat cgacggttac gtctcttttt
     1921 ccaaaaaatt gtgaatctgc aaccgcggca atgcctactt ctcatcccac ccgattcata
     1981 accacaagca atttttcttt tgtgtttaat gcacataatc aactaattaa gttttactca
     2041 ttggcatagc ttcct
//
LOCUS       DAOSHP010000155         1890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_61737_length_1890_cov_7.859946, whole genome shotgun sequence.
ACCESSION   DAOSHP010000155 DAOSHP010000000
VERSION     DAOSHP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61737_length_1890_cov_7.859946"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..792
                     /locus_tag="PLB33_09365"
     CDS             151..792
                     /locus_tag="PLB33_09365"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOG63776.1"
                     /translation="MESISCQEMKEMDSYAINTVGIPSIVLMENAALKVINNIDLNYN
                     NYTVVCGRGNNGGDGLAIARHLIIKGKKVRIYIAGDLKSGTEDFNINLNILKNLKAEI
                     INIKDEKELLQLKEDLSVSDITIDALFGIGLKRNIEGLYLEIIKMINNESKQIISVDI
                     PSGIDGDTGKPMGICVKADKTVTFHKMKKGLLNCGNYTGEIIVEDIGIPNIQF"
     gene            complement(845..>1890)
                     /locus_tag="PLB33_09370"
     CDS             complement(845..>1890)
                     /locus_tag="PLB33_09370"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOG63777.1"
                     /translation="EEYDYEQLILCQDKSTGLKAIIAIHDTTLGPALGGTRICEYESD
                     EEAIEDALRLARGMTYKNAAAGLNLGGGKAVIIGDPRKIKSEALFRAFGRFVEGLNGR
                     YITGEDMNATQEDASYINCETEYIVGLETGSGNPSPVTAYGVFKGIQAALNEVYGSDD
                     LTGKTVAIQGLGAVGRRLAERLHEAGAKLYVTTRDKAKIDKAVAELGATAVSLDEIYG
                     VECDVFSPCARGAIINDKTIEQFKCKIIAGAANNQLAESRHGDMLHEKGILYVPDYII
                     NSGGVINIIDDISGREYNRENAMKNTARIYDSCKKVFEIAKRDNIPTYKAADIMAEER
                     IAAVGRIKKIYNR"
BASE COUNT          593 a          341 c          314 g          642 t
ORIGIN      
        1 ttattcttgt ttattcggct aaaattttat ataataaaaa ataagtattt ttatcggagg
       61 tgctgcacag atgggtagga aaccgaggaa aaatttgatg agcaggcacc tgaaatttct
      121 gagattgaga gtaataaggg agaatggatt atggaatcga taagttgtca agaaatgaag
      181 gaaatggaca gctatgccat taacacagtt ggtataccaa gtattgtatt aatggaaaat
      241 gcagcgctta aagttataaa taatattgat ttgaattata ataattatac tgttgtatgc
      301 ggaagaggaa acaacggcgg tgatggactt gctattgctc gccatttaat tataaaaggt
      361 aaaaaagtaa ggatatatat tgccggagat ttaaaaagcg gcacagaaga ttttaatatt
      421 aatttaaata ttttaaaaaa tcttaaggcg gaaatcataa atataaaaga tgaaaaagaa
      481 cttctgcaat taaaggaaga tttatctgtc agcgacatta caattgatgc attgtttgga
      541 attggtctaa aaagaaatat tgaaggtttg taccttgaaa taattaaaat gataaataat
      601 gaatcaaaac aaattatatc agttgatatt ccatccggaa tagacgggga tacaggaaag
      661 ccaatgggta tttgcgtaaa ggctgacaaa accgttacat ttcataaaat gaaaaaggga
      721 ctgttaaatt gcggtaacta taccggcgaa attattgtag aagatatagg cataccaaat
      781 atacaatttt aaaaaccaat cttccgaaat ttacggttga ttggtttaat actaaatttt
      841 agatttatct attatagatt tttttaattc ttcctactgc tgctattctc tcttctgcca
      901 taatatcagc agccttatat gttggaatat tatctctttt agctatctca aacaccttct
      961 tgcaagaatc ataaattctt gcggtatttt tcattgcatt ttccctattg tattcacgtc
     1021 ccgatatatc atcaattata ttaatcacac cgccgctgtt tataatataa tccggcacat
     1081 aaagtattcc tttctcatgc agcatatcac cgtgtcttga ctcagctaac tgattgttgg
     1141 ctgcgccggc tatgatttta catttaaatt gttctattgt tttatcgttt attatagcac
     1201 ctctagcaca aggtgaaaat acatcacatt caacaccata tatttcatcc aggcttactg
     1261 ctgttgcacc cagttctgct acagctttat caatttttgc cttatctctt gtagtaacgt
     1321 ataatttagc acctgcttca tgtaaacgct cggctaaacg tctgccgacg gcacctagac
     1381 cttgaattgc tacggttttt ccggttaaat catcgctgcc gtaaacttca tttaaagctg
     1441 cctgtattcc tttaaatact ccataagcgg ttacaggtga aggattgccg cttccggttt
     1501 ctaaacctac tatatattct gtttcacaat taatatatga agcatcttct tgtgttgcat
     1561 tcatatcttc tccggttatg tatctgccat ttaaaccttc gacaaatctg ccgaaagctc
     1621 taaacaatgc ttcgcttttt atcttccttg ggtctcctat aataactgct ttcccgccgc
     1681 caaggtttaa tcctgcagct gcatttttat atgtcatacc cctggctaat cttagcgcat
     1741 cttcaatcgc ttcttcatca gactcatatt cgcaaattct tgttcctcct agtgccggac
     1801 ctaatgtagt atcatgaata gcaataattg cctttaatcc ggtactttta tcttgacaaa
     1861 gtattaattg ttcgtaatca tattcttcca
//
LOCUS       DAOSHP010000156         1878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_62387_length_1878_cov_10.906747, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000156 DAOSHP010000000
VERSION     DAOSHP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62387_length_1878_cov_10.906747"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            984..1583
                     /locus_tag="PLB33_09375"
     CDS             984..1583
                     /locus_tag="PLB33_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOG63778.1"
                     /translation="MSVATNKKKLILDIATQLFTRFGFAKTSLDEIAAAAQIAKGTVY
                     YYFPSKEDLFIASVEAKTREYFTILRQHINSTSGFEEKLTEFLSFPIKLIYENMPILI
                     ECLINIPQSYQEQLIQFRSKSREVMMQILSEIIQLGKQEDLIDERFPEDRLCEVIMDW
                     FMVGEPSLKIVDVPRLLTMIERDNDFIIKMLLYGIVKRG"
     gene            1587..>1878
                     /locus_tag="PLB33_09380"
     CDS             1587..>1878
                     /locus_tag="PLB33_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOG63779.1"
                     /translation="MKKTLTLFVLLTLLASCVFAIDLENSISKAKQNNKELLMAMEEI
                     KKADESYKQVRGTAYPQLNLQGAYGLSKTYYPDSAIPAKMDISSGLDDTASDN"
BASE COUNT          627 a          307 c          355 g          589 t
ORIGIN      
        1 tttacaaaga gaaaaagcaa ttaaaggtga attgagaata aacagtgaag ggtgaaaagt
       61 tgagccgccc agtaaaaatt gaaataataa atccctgcaa acttttaaaa aaatcctttt
      121 aatttgggtt gccagcaaat ccggaaagta taatacttat gatgcataag cgtagttaaa
      181 attatcgtcc agaacatatt tattaaagtt ctaataattc ctgacttaca tagaggacgg
      241 aaatgacgag gaagtcattt attccggaaa aatatgataa ctctaattgt tttgctatct
      301 tcgccctcgt tctcttctgc cttgcaagct ccatcctgat ttcttcactt gttataattt
      361 attgacttga catcatcttc tcctttattt ttacttttat taacaaagtc cttccacttt
      421 cattttgctt ctctatcatc ttatattatt atattgttac ctcttattaa tgtccatttt
      481 atttttgcta aaccttttgt caccccataa gtacattttc tattcggata taatttagaa
      541 gaagaaaaca gcgcagaaga tgaagacaag gaaaatgcgg agaaaaaaga gttggcaaga
      601 agataaaaaa gattataata tcaatgtgaa acatctggag cgcctgcccg cggggaaaga
      661 tgtgaactgg tcaaatagca ggcaatattg atgatcagcc caataataag agctacgcaa
      721 gtagctctta tttattgtat gctcctatgc cttgcctaat atcgttaata tcatttaagc
      781 gttgaattaa gatattatca gagtatattt cggcaataat cataaggatt tggcaatttt
      841 ttgacaaatg ttatggcaac tgataacagt ctccatttca taaattttgt cttgacgatt
      901 atatactgtt tgactgttat gggtcagttg ttcagttata aagctgtaac atacagatag
      961 acaataaaat ataaggaagg agaatgagtg ttgcaacgaa caaaaaaaag ttgatactgg
     1021 atatagctac tcagctattt acccgtttcg gctttgccaa gacctccctg gatgaaattg
     1081 ctgctgctgc tcagatagct aagggaacag tatattatta ctttcccagc aaggaagacc
     1141 tctttattgc ttcagtggaa gccaagacaa gagagtattt cacaattctg agacagcata
     1201 tcaattctac ttcaggtttt gaagagaagc tgacggaatt tttaagtttt cctatcaaac
     1261 tgatttacga aaatatgcct atcctgattg agtgtttaat taatataccg caaagttatc
     1321 aggaacaatt gattcagttc cgaagtaaaa gcagggaagt gatgatgcag attctttctg
     1381 aaataatcca actgggaaaa caggaagatt taattgatga gcgcttcccg gaagaccgtc
     1441 tttgtgaagt aattatggat tggtttatgg ttggtgaacc gagtttgaaa atagtagatg
     1501 ttccccgctt attgacaatg attgagcggg ataatgattt tattattaag atgctactct
     1561 atggaattgt taaacgagga taaaaaatga aaaagacatt aactttattc gtcctgctaa
     1621 cgcttttagc aagttgtgta ttcgccattg acctggaaaa tagcatctcc aaagctaaac
     1681 agaataacaa ggaactgctg atggcaatgg aagaaataaa gaaagcagat gagtcatata
     1741 aacaggttcg tggcactgct tatccacaat tgaatttaca gggtgcttac ggtttaagta
     1801 agacctatta tcctgattca gctatacctg ctaagatgga tattagttcc ggtttggatg
     1861 ataccgcaag cgataatg
//
LOCUS       DAOSHP010000157         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_62545_length_1875_cov_11.596703, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000157 DAOSHP010000000
VERSION     DAOSHP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62545_length_1875_cov_11.596703"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..357)
                     /locus_tag="PLB33_09385"
     CDS             complement(154..357)
                     /locus_tag="PLB33_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63780.1"
                     /translation="MRTVENLNELQANMKTLDKYILSKKEPEYNFGLNLVKKGTCSLQ
                     GFQTDFMEIYLRVYMEKYLPFTS"
     gene            complement(513..>1875)
                     /locus_tag="PLB33_09390"
     CDS             complement(513..>1875)
                     /locus_tag="PLB33_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOG63781.1"
                     /translation="FRFICDIICYENGGTTQGKSNNEFDLLKEYFSGQNKNALVNIKT
                     LEDYFDCWCSFKGDNTVDKFLERFISYEHQAGMIKFEKRYAINIFNDCVRNYADISGN
                     GNRLFPLNRIILLYAIICYLLNKTMISNDELIRRLRIVNNLIQNSEDEISDSELRTSG
                     NRMPAIFKQVSTIIKTGRIDDTIEKNFNLIQLAEESLKIIWVENNPEKAEMLFELEDH
                     DLLQGQISIIGLEHLDYYKRFQSLFSCKWDLIDCALMSIGNYGQREKNGWRYQLGSKR
                     NMKAWRNLFHKSGNDGFNRTKDILAVLLSRADTFTDEILSEIIDKYIYDCETSNNYEW
                     RYYYIKYDVFRPGSFGKYYWSMFDNKPYEFSVMKTESKVSENTYQPFLMTVYKNKLSK
                     DSYGQRAIDGDNYIVCLNSAYVVKSIATDEEVERITISQNEKGIDTEDRIKKFQMTLG
                     KIV"
BASE COUNT          583 a          370 c          242 g          680 t
ORIGIN      
        1 agtgataaga ttattataac acttaatatt ataacaatgt atacaaatta tctatatttc
       61 ctctacagca ataatctctt ggatatttgt taaccagccc agatttattg aaatattatc
      121 ccccatattt ctcattctta tctttgatct cagttatgat gtaaacggca agtacttttc
      181 catataaaca cgcaaatata tttccataaa atccgtctgg aacccctgca aagaacaagt
      241 gccttttttt actaagttta gcccaaaatt atattccggt tctttttttg ataatatgta
      301 cttgtccaat gttttcatat tggcctgtaa ttcatttaaa ttctctactg tcctcatttt
      361 gaaactctct tctattataa aatttgcaaa aattaatatc aataattcaa aaataacaat
      421 caactcaata tgtctaattt tataaacagc cctacaaata actgatgtaa gctaacaact
      481 caaataagtt aaattttgct ggcttccaac ctttaaacaa ttttccccaa ggtcatctga
      541 aattttttaa tcctgtcttc tgtgtcaata cccttttcgt tttgtgaaat agttatcctt
      601 tcgacttcct catcagtagc aatgctttta acaacatatg cagagttcaa gcaaactata
      661 taattgtcac catcaatggc gcgttgccca taagagtctt ttgatagctt atttttataa
      721 actgtcatta aaaatggctg atatgtgttt tcagaaacct tagactctgt tttcataacg
      781 gaaaactcgt atggcttatt atcaaacata ctccagtaat atttcccgaa acttccaggt
      841 ctgaaaacgt cgtacttaat gtagtaatat ctccactcat agttattgct agtttcgcaa
      901 tcatagatgt atttgtcaat tatttctgac aaaatctcat cggtaaacgt atcagcacga
      961 gatagcaaaa ctgcgagaat atcctttgtt ctgttaaatc catcattacc gcttttatgg
     1021 aacagattcc tccaagcctt catgttcctt ttagaaccta attgatatct ccatccgttt
     1081 ttttctcttt gcccatagtt tccaatagac ataagtgcac aatcaataag gtcccactta
     1141 caggagaaca atgattgaaa acgcttataa taatcaaggt gttccaggcc aataatactg
     1201 atttgtcctt gtagcaaatc atggtcctct aattcgaata gcatttcagc cttctccggg
     1261 ttattttcga cccatataat ctttaaggat tcctccgcta attgaattaa gttaaagttt
     1321 ttctcaatag tgtcatcgat tctgccggtt ttaataatgg tactaacctg cttaaaaata
     1381 gccggcattc tatttccgct tgttctgagt tcactatcgc taatctcatc ttcggaattt
     1441 tgtattaagt tgttaactat tcttagtctt cgtattaatt catcattgga tatcattgtt
     1501 ttattcaaga gatagcatat aatagcatat aataaaatta tcctgtttaa cggaaaaagc
     1561 ctgttaccat tgcctgatat atcagcgtaa ttacgcacac agtcattgaa aatgttaatt
     1621 gcgtaccttt tttcaaattt tatcattcct gcttggtgtt catatgatat aaacctttcc
     1681 aaaaacttat caactgtgtt atctccctta aaactgcacc aacagtcaaa atagtcttca
     1741 agtgttttga tattaaccaa ggcattttta ttttgaccag aaaaatattc ttttaaaagg
     1801 tcgaattcat tgttgctctt accttgagtt gtcccgccat tttcgtaaca aataatatca
     1861 caaatgaacc tgaag
//
LOCUS       DAOSHP010000158         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_62918_length_1868_cov_10.020960, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000158 DAOSHP010000000
VERSION     DAOSHP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62918_length_1868_cov_10.020960"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="PLB33_09395"
     CDS             <1..1457
                     /locus_tag="PLB33_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOG63782.1"
                     /translation="LIGAGQATALLLEELESNNPNNYLVKCIIDDDDAKIGRSMYKIN
                     IVGNTAKILEMSEKHEIEIIIVVIPSIDKENKKRILDICSKTQCKLKILPEFCSLIPY
                     DSINSFSESIRDVQIEDLLGREPIALDCELTKGYLNNKTVLVTGGGGSIGSELCRQIA
                     SCYPKKLIILDEYENNAYEIQRDLLCKYGDKLNLEIEIASVRDGRKLEKVFLSNEISI
                     IFHAAAHKHVPLMEHNPEEAVKNNIIGTYNTVRMADKFIADKFILISTDKAVNPTSIM
                     GATKRICEMIVQSYAKHSSTNFSAVRFGNVLGSNGSVIPLFKQQIKEGGPVTITHENI
                     TRYFMTIPEAVQLVLKAASIAEGGEIFVLDMGEPVKIKDLAYNLIRLSGLIPEDDIKV
                     EYIGLRPGEKLYEELLIAGDASQTKTEIEKIFIEKPMEFDEKQFFESLEKMVMAAKNN
                     ERDSIVSLIEKTVPTYKKIPEGQEKGFNVQQTVI"
     gene            1487..>1868
                     /locus_tag="PLB33_09400"
     CDS             1487..>1868
                     /locus_tag="PLB33_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG63783.1"
                     /translation="MYEEEFNEIDLREIFQIIRKYLLLIIIIPMVFAGIGAVVSIYLI
                     QPVYESSTTLIVREDKDAEEEISISDVNLSKSLVYTYAEMAKSYTVLDNTAKTLNIDK
                     LNRSSITVSPVKDTQILKVAVQNTD"
BASE COUNT          691 a          273 c          394 g          510 t
ORIGIN      
        1 tgctcatcgg tgccggacaa gcgactgctt tattgttaga agagctcgaa tcaaataacc
       61 ccaataatta cttggtgaaa tgcatcatag atgatgatga tgcgaaaatt ggaaggtcta
      121 tgtacaaaat aaatattgtg ggaaatacag caaagatttt agaaatgtct gaaaagcatg
      181 aaattgaaat aataattgtt gttataccct ccattgataa agaaaataaa aagcggattc
      241 tagatatttg ttcgaaaaca cagtgcaagc taaaaatatt gcctgaattt tgttcattaa
      301 taccatatga tagtattaat tcattttcag aaagcataag agacgtacag atagaagatc
      361 ttttgggtag agagccgatt gcacttgatt gtgaattgac aaaaggttat ttgaataata
      421 agacagtgct tgttaccgga gggggagggt ccatagggtc agaactgtgc agacagatcg
      481 catcttgtta tccgaaaaag ctgattatat tggatgaata tgaaaataat gcttatgaga
      541 ttcaaagaga cttgctatgt aaatatggag ataagttaaa ccttgaaata gaaattgctt
      601 ctgtgaggga tgggcggaaa ttagaaaaag tatttttatc caatgaaata agtattatct
      661 ttcatgctgc tgcccataag catgtgcctc ttatggagca caacccggag gaagctgtaa
      721 aaaacaacat aataggtact tataatactg taaggatggc agacaagttt attgcagaca
      781 agtttatttt gatatccacc gacaaggcag tgaaccccac aagtattatg ggagcaacaa
      841 aaaggatttg tgaaatgata gtccagtcat atgcaaaaca cagctctaca aacttttcag
      901 ccgtaaggtt tggcaatgta ttaggaagca atggctccgt aataccactt tttaagcaac
      961 agataaaaga aggaggtcct gttaccataa cacatgaaaa tataaccaga tattttatga
     1021 caatacctga agcagtgcag cttgtactta aagctgcatc aattgccgaa ggaggagaaa
     1081 tatttgtttt agacatgggt gaaccggtga agataaagga tttggcctat aatcttatca
     1141 ggttgtcagg tcttattcct gaagatgaca taaaggttga atatatcggt ttaagaccgg
     1201 gggaaaagct ttatgaagaa cttttgatag ctggtgatgc atctcaaact aaaacagaaa
     1261 ttgaaaagat atttatagaa aaacctatgg aatttgacga aaagcaattt tttgaatcat
     1321 tggaaaagat ggtgatggct gccaagaata atgaaagaga tagcattgta agcctaatag
     1381 aaaaaactgt gcctacctac aaaaaaatac cagaagggca ggagaaaggg tttaatgttc
     1441 aacaaacagt catataaaca atcataaaac ggaggaaaag gcggctatgt acgaagaaga
     1501 gttcaatgaa atagacttga gggagatatt tcagataatt agaaaatatc ttttattaat
     1561 aattataata cctatggtat ttgcgggaat cggagctgtt gtaagcattt atttaatcca
     1621 gcccgtatat gagtcttcta ctacattgat agttagagaa gacaaggatg ccgaggagga
     1681 aataagcata tcagatgtta atttaagcaa aagcctggtt tatacgtatg ctgaaatggc
     1741 caaatcctat acggtattgg ataacactgc caaaacattg aatatagaca agcttaacag
     1801 aagtagcatt accgtatcac ctgttaagga cacacagata cttaaggttg cagtccaaaa
     1861 tactgacc
//
LOCUS       DAOSHP010000159         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_62920_length_1868_cov_9.085494, whole genome shotgun sequence.
ACCESSION   DAOSHP010000159 DAOSHP010000000
VERSION     DAOSHP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62920_length_1868_cov_9.085494"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="PLB33_09405"
     CDS             <1..1457
                     /locus_tag="PLB33_09405"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOG63784.1"
                     /translation="LIGAGQATALLLEELESNNPNNYLVKCIIDDDDVKIGRSMYKIN
                     IVGNTANILEMSEKHEIDIIIVVIPSIDKENKRRILDICSKTQCKLKILPEFCSLIPY
                     DSINSFTESIRDVQIEDLLGREPIALDCELTKGYLNNKTVLVTGGGGSIGSELCRQIA
                     SCYPKKLIILDEYENNAYEIQRDLLCKYGDKLNLEIEIASVRDGRKLEKVFISNEINI
                     IFHAAAHKHVPLMEHNPEEAVKNNIIGTYNTVRMADKFIADKFILISTDKAVNPTSIM
                     GATKRICEMIIQAYARHSSTNFSAVRFGNVLGSNGSVIPLFKQQIKEGGPVTITHENI
                     TRYFMTIPEAVQLVLKAASIAEGGEIFVLDMGEPVKIKDLAYNLIRLSGLIPEDDIKV
                     EYIGLRPGEKLYEELLIAGDASQTKTEIEKIFIEKPMEFDEKQFFESLEKMVMAAKNN
                     ERDSIVSLIEKTVPTYKKIPEGQEKGFNVQQTVI"
     gene            1487..>1868
                     /locus_tag="PLB33_09410"
     CDS             1487..>1868
                     /locus_tag="PLB33_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOG63785.1"
                     /translation="MYEEEFNEIDLVEIFKIIRKYLLLIIIIPMVFAGIGAVVSIYLI
                     QPVYESSTTLIVREDKDAEEKISISDVNLSKSLVYTYAEMAKSYTVLDNTAKTLNIDK
                     LDGNSITVSPVKDTQILKVAVQNTD"
BASE COUNT          701 a          273 c          390 g          504 t
ORIGIN      
        1 tgctcatcgg tgccggacaa gcgactgctt tattgttaga agagctcgaa tcaaacaacc
       61 ccaataatta cttggtgaaa tgcattatag atgatgatga tgtgaaaatt ggaaggtcta
      121 tgtacaaaat aaatattgtt ggaaatacag caaatatttt agaaatgtct gaaaagcatg
      181 aaattgacat aataattgta gttataccct ccattgataa agaaaataaa aggcggattc
      241 tagatatttg ttcgaaaaca caatgcaagt taaaaatatt gcctgaattt tgttccttaa
      301 taccatacga tagtattaat tcatttacag aaagcataag agacgtacag atagaagatc
      361 ttttgggtag agagccgatt gcacttgatt gtgaattgac aaaaggttat ttgaataata
      421 agacagtgct tgttaccgga gggggagggt ccatagggtc agaactgtgc agacagatcg
      481 catcttgtta tccgaaaaag ctgattatat tggatgaata tgaaaataat gcttatgaga
      541 ttcaaagaga cttgctatgt aaatatggag ataagttaaa ccttgaaata gaaattgctt
      601 ctgtgaggga tggacgtaaa ttggaaaaag tatttatatc aaatgaaata aatataatct
      661 ttcatgcagc tgcccataaa catgtgcctc ttatggagca taacccggag gaagccgtaa
      721 aaaacaacat aattggtact tacaatactg taaggatggc agacaaattt attgcagaca
      781 agtttatttt aatatccacc gacaaggcag tgaaccctac aagtattatg ggagcaacaa
      841 aaaggatttg tgaaatgata atccaggcat atgcaagaca cagctctaca aatttttcag
      901 ctgtaaggtt tggcaatgta ttaggaagca acggctccgt gataccactt tttaagcagc
      961 agataaaaga aggaggacct gttaccataa cacatgaaaa tataaccaga tactttatga
     1021 caatacctga agcagtgcag cttgtactta aagcagcatc aattgccgaa ggaggagaaa
     1081 tatttgtgct ggacatgggt gaaccggtga agataaagga tttggcctat aatcttatca
     1141 ggttgtcagg tcttattcct gaagatgaca taaaggttga atatatcggt ttaagaccgg
     1201 gggaaaagct ttatgaagaa cttttgatag ctggtgatgc atctcaaact aaaacagaaa
     1261 ttgaaaagat atttatagaa aaacctatgg aatttgacga aaagcaattt tttgaatcat
     1321 tggaaaagat ggtgatggct gccaagaata atgaaagaga tagcattgta agcctaatag
     1381 aaaaaactgt gcctacctac aaaaaaatac cagaagggca ggagaaaggg tttaatgttc
     1441 aacaaacagt catataaaca atcataaaac ggaggaaaag gcggctatgt acgaagaaga
     1501 gttcaatgaa atagacttgg tggagatatt taagataatt agaaaatatc ttttattaat
     1561 aattataata ccaatggtat ttgcgggaat tggagctgtt gtaagcattt atttaatcca
     1621 gccggtgtat gaatcctcta ctacattgat agtaagagaa gacaaggatg ccgaggagaa
     1681 aataagcata tctgatgtca atttaagcaa aagcctagtt tatacatatg cagaaatggc
     1741 caaatcctat actgtattgg ataacacagc caaaaccttg aacatagaca agcttgatgg
     1801 caatagtatt acagtatcac ctgttaagga cacacagata cttaaggttg cagtccaaaa
     1861 tactgacc
//
LOCUS       DAOSHP010000160         1847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_63972_length_1847_cov_6.026786, whole genome shotgun sequence.
ACCESSION   DAOSHP010000160 DAOSHP010000000
VERSION     DAOSHP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63972_length_1847_cov_6.026786"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..948
                     /locus_tag="PLB33_09415"
     CDS             76..948
                     /locus_tag="PLB33_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOG63786.1"
                     /translation="MNKTKGLTFGILAGFIYGFTPILGKLTYLEGSNPISLTFYRNLL
                     SIPFFFVMLKYNNVPLKVTKSQTKQLAILGLLASATALSLYGSYNYISVGMSTTIHYI
                     YPVLVTAACIIIFGEKISRDKVVSLILSTMGITLFFEGSINITGILIAFMSGVFFAGY
                     LLFMDKSGLNTIYQFKITFYTAAFSSMYLFIFGIISKNLVFTMTFKGWFFTVLVAVFV
                     SFFANTFVALGVKYVGPTVTSIVGMLEPITSIVMGILFINEPITLRNILACVLILLGV
                     LIVSLSKEKASEDN"
     gene            complement(978..>1847)
                     /locus_tag="PLB33_09420"
     CDS             complement(978..>1847)
                     /locus_tag="PLB33_09420"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOG63787.1"
                     /translation="HGGPFANIAHGCNSVIATRMSCKIADCAVTEAGFGADLGAEKFV
                     DIKCRKTGLKPSAVVIVASVRALKHHGGAAKADLGKENLEALDKGLPNLLKHVENITQ
                     VFKIPAVVAINKFPTDTEAELKLIKEKCMELGVNVKLSEVWGKGSEGGVDLANEVIRL
                     TEQESSLEFAYDLDISIKDKIRAIARKIYGADDVEFSSKASKEIANFEKLGFGNLPIC
                     MAKNQYSFTDDSKVFGRPTGFNITIRDITISAGAGFLVALTGDIMKMPGLPKVPAAEN
                     IDIDVDGRIKGLF"
BASE COUNT          544 a          358 c          298 g          647 t
ORIGIN      
        1 tgacccatgc ccctgcggtt caggattaaa atataaaaag tgctgcggaa aataaaaatg
       61 atataggagg gaattatgaa taaaacaaaa ggcttaacat tcggtatctt agccggattt
      121 atttatggtt tcactcctat attgggaaaa ttaacgtatt tggaaggaag caatcccata
      181 tcacttacat tctatagaaa tcttctttcc attccgtttt tttttgttat gttaaaatat
      241 aataatgtac ctttaaaagt tacaaagagc cagacaaaac aattagcaat attaggattg
      301 ttagcttccg ctaccgcact cagcctttac ggctcctata attatatatc tgttggaatg
      361 agcacaacta ttcattatat ttatcctgta ttggttactg ctgcgtgtat tattattttt
      421 ggtgaaaaaa taagcagaga caaggtagtt tctttgatac tatccacaat gggaattact
      481 ttatttttcg aaggaagtat caatataacg ggaatactga tagcttttat gtcaggtgta
      541 ttttttgcag gatatttatt atttatggac aaaagcggtt tgaatacaat atatcaattt
      601 aaaataacat tttatacagc cgccttttca tctatgtacc tgtttatttt tggtataata
      661 agcaaaaacc ttgtttttac aatgacattt aagggatggt tttttacggt cttagttgca
      721 gtctttgttt cattttttgc aaatacattc gtagccctgg gagtaaaata tgtaggtcct
      781 acagttacga gtatagttgg aatgcttgaa cctattacaa gcatagtgat gggtatattg
      841 tttataaatg aaccgataac attaagaaat atccttgcat gcgtgctgat attgctcggt
      901 gttctgatag tgtcgctatc gaaagaaaaa gctagcgagg ataattaata aagaagggga
      961 cattccgtcc cctctcatta aaacagtcct tttatccttc cgtcaacatc tatatctata
     1021 ttttctgctg caggtacctt aggaagcccc ggcatcttca ttatatctcc cgtcaatgca
     1081 actaaaaatc ctgctccggc tgatattgtt atatctctga ttgttatatt aaaacctgta
     1141 ggcctgccaa atacctttga atcatcagta aaggaatatt ggtttttagc catgcatata
     1201 ggaagatttc caaatccaag tttttcaaaa ttagcaattt cttttgatgc ttttgatgaa
     1261 aattccacat catcggcacc atatatcttt cttgcaattg cccttatctt atccttgatg
     1321 gaaatatcta aatcataggc aaattccaat gagctttcct gctctgtaag tcttataact
     1381 tcattggcaa gatcaactcc gccttcgctt cctttgcccc atacttctga cagctttaca
     1441 tttacaccca actccataca tttttctttt atcaatttca attctgcttc cgtatctgta
     1501 gggaacttgt ttattgcaac aacagcaggt attttaaaca cctgagtaat attctcaaca
     1561 tgctttaata aattaggaag tcctttatcc aatgcttcca ggttctcttt tcctaaatct
     1621 gcctttgctg ctccaccatg gtgtttcaat gctctaaccg atgcaactat aacaactgca
     1681 cttggtttta atcctgtttt tctgcatttt atatccacaa atttttcagc tcccaagtct
     1741 gctccaaaac ctgcctcagt taccgcgcag tcagcaattt tacaagacat tcttgtagca
     1801 attactgaat tacagccgtg ggctatgttt gcaaaaggac ctccgtg
//
LOCUS       DAOSHP010000161         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_64697_length_1833_cov_9.641170, whole genome shotgun sequence.
ACCESSION   DAOSHP010000161 DAOSHP010000000
VERSION     DAOSHP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64697_length_1833_cov_9.641170"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(85..168)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(273..1136)
                     /locus_tag="PLB33_09425"
     CDS             complement(273..1136)
                     /locus_tag="PLB33_09425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOG63788.1"
                     /translation="MKNNSIKLKEILDINDFNDINNLQNICTDYDKTHLKIEIDYKLG
                     KGDKNSENMNFINEFMFYDDNKLVGYAGICDFGGDEIEINGMVHPDYRGRGIFTRLFS
                     LVKDEFYKRDNKAMLLLSDNNSIAGINFIKNITDDYDHSEYDMYLDMDVVHNLSFDNL
                     IFRKAVYEEVNKITKENFEFFNANDIEEISSGSTYIIETGNEIIGKVRLEIIDDIGGI
                     YGLEILPDYRGKGFGRELLIQSINKLKEYKVKTVNLQVETENRNALNLYKSCGFKENY
                     IMDYYRLIKGI"
     gene            complement(1167..1646)
                     /locus_tag="PLB33_09430"
     CDS             complement(1167..1646)
                     /locus_tag="PLB33_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOG63789.1"
                     /translation="MNTLIAYGTKYGCTEKCATELSKEIDGNVEIVNLKNNNKIDLNK
                     YDRVIIGGSVYIGKIQKEVTEFINSNLNELFKKQIGLFICGMQEEGETLDNEITDNFH
                     PDLLASAKSVQNFGGEFIFNKMNFMEKVIIKKIIKITSDKSDIKHDNIKKLAMEMNN"
BASE COUNT          552 a          327 c          223 g          731 t
ORIGIN      
        1 cttataatat atttgggata aatacgaatt aaaaaagctc tgtatgggtc gccaaacaga
       61 gccaatactt ttatgtatct gcattttggt aacccggctt ccttggtttt taccttaagg
      121 cccgtggctt tgcgtcctca gctttcacta agtttgcctt tatcaattcg ctaatttatt
      181 cctgcaaatt cattatattc cttttaggta ttttgtcaaa taatatattt ttaggatttt
      241 atttatttga atctatcgcg aactgaagat gtttaaatac cttttatcag cctgtaataa
      301 tccatgatgt agttttcttt gaaaccacat gacttataaa gatttaaagc gtttctattt
      361 tcagtttcta cctgcaggtt aacggttttt actttgtatt cctttaactt attaattgac
      421 tggatcaata attctctccc gaagccttta cctctatagt caggaagaat ttccaaaccg
      481 tatatgcccc caatatcatc aataatttct aatcttactt tccctattat ctcattgccg
      541 gtttcaatta tataagttga gccggaagaa atctcttcaa tatcatttgc attaaaaaac
      601 tcgaagtttt cttttgtgat tttgtttact tcttcataaa cagccttcct gaaaatcaaa
      661 ttatcaaaac tcaaattatg tactacatcc atatccaaat acatgtcata ttcagaatga
      721 tcataatcat cggtgatgtt ttttataaaa ttaataccgg caatagaatt attgtcactt
      781 aacaaaagca ttgccttgtt atccctctta tagaattcat ctttgaccaa agaaaacagt
      841 cttgtaaaaa ttcctcgccc tctgtaatcc ggatgtacca tgccgtttat ttcaatctca
      901 tctccgccaa aatcacatat tccggcatat ccgacgagtt tattatcatc ataaaacata
      961 aattcattta taaaattcat attttcgctg tttttatcac ccttgcccag cttgtaatca
     1021 atttctattt tcaaatgtgt tttgtcataa tcagtacaaa tattttgtaa attattgatg
     1081 tcattaaagt cattaatatc taatatttct tttaatttaa tcgaattgtt tttcatttat
     1141 acctcatcaa ttaatgaatt atcatactaa ttattcattt ccatcgctaa ttttttaatg
     1201 ttgtcatgtt ttatgtctga tttgtctgat gttattttga tgattttctt aattataact
     1261 ttttccatga agttcatctt gttaaatata aattctcccc cgaaattctg aaccgatttt
     1321 gcactggcta acagatccgg atgaaagtta tcagttattt cattatcaag ggtttctcct
     1381 tcttcctgca taccgcaaat aaatagaccg atttgctttt taaagagttc gtttaagttt
     1441 gaatttataa attccgttac ttctttttgg atttttccaa tatagacaga accgccaatt
     1501 attacacggt cgtatttgtt caggtcgatt ttgttgttgt tttttaagtt aactatttca
     1561 acattaccgt caatttcctt tgataattct gttgcacatt tttctgtaca tccgtacttt
     1621 gttccataag caattaatgt attcatattc cctccaaatt aaatattaaa tacttattat
     1681 aatataataa tactgcgaac taaagatatt gtaaataact tatcaaccat aaaatagttt
     1741 agtttatttt ttaagaatta acctattgac atttacgctg cgtaaaggta tacacttgtc
     1801 ttaggagtga tgaaatggaa tatacaatta ata
//
LOCUS       DAOSHP010000162         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_66855_length_1793_cov_6.882048, whole genome shotgun sequence.
ACCESSION   DAOSHP010000162 DAOSHP010000000
VERSION     DAOSHP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66855_length_1793_cov_6.882048"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLB33_09435"
     CDS             complement(<1..203)
                     /locus_tag="PLB33_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOG63790.1"
                     /translation="MAKKNLGLAQKVLIGLAIGFVVGLIVHYMPKGTLRDDVLIDGVF
                     QFVGQVFLRGIMMCVVPLVFVSLV"
     gene            complement(454..1191)
                     /locus_tag="PLB33_09440"
     CDS             complement(454..1191)
                     /locus_tag="PLB33_09440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOG63791.1"
                     /translation="MFRYFCADENIKNNTVNVVGGDAKHLKTILRAQPSDKISVVTES
                     KEYIAEIKEINTENIVCTLVEEIFSNNETKINITLCQGIPKKTKMETIIQQNVELGVK
                     SFIPLITERTVVKLNEKDREQKKLDRWQKIAKESAKQSKRNIIPTVEPIITVSELIEK
                     LKNIDAEIIVPYELEDVKILKDVLKEPKDNYYIVIGPEGGFDIKEIEMFQEIGAHIVT
                     LGKRILRTETAGIVTASVIMYACNEMV"
     gene            complement(1268..>1793)
                     /locus_tag="PLB33_09445"
     CDS             complement(1268..>1793)
                     /locus_tag="PLB33_09445"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOG63792.1"
                     /translation="IDPGMSFGTGQHHTTRLCIEQIIKYMNKDMEVLDLGCGSGILSI
                     TSLLLGAKKCVGVDIDENSVRIARENAELNNINQDRFTVYLGDVTEDKALQEKIGYNK
                     YDMIAVNIIAQVIMSMSYTFPKFLKKGGLVIASGIIKKYLQEVTDNFENLGFEIIEIN
                     ESEEWVSVTAKLPS"
BASE COUNT          512 a          374 c          233 g          674 t
ORIGIN      
        1 actaaggaaa caaataccaa aggaactacg cacatcatta ttcctcttag gaatacctga
       61 ccaacaaatt ggaatacacc gtcaattaaa acatcatccc taagtgtgcc ttttggcata
      121 taatgaacta taagacctac gacaaaacca atggctaacc ctataaggac tttctgtgct
      181 aatcccagat ttttctttgc cattataacc ctccttttaa aaatttaaaa aactttataa
      241 acaatcaact gtttacgttt acattgtata tattcccgta taatttgtca atcaaatcgt
      301 atgaattttg tccaacaaat atttacaatt tatacaaaac ctccttttat aataaacgta
      361 ttagataata gtatgtttta ttccttttgt ttcttttgta aaataaaaaa acccggaaaa
      421 ttccgatgaa ctttccggag aacttttata ccgctatacc atctcgttac aggcatacat
      481 tatgacagaa gctgttacga taccggcagt ttcagtcctt agaattcttt tgcctaaggt
      541 tacgatatga gcgccgattt cctgaaacat ttcaatttct ttaatatcaa aacctccctc
      601 agggcctatt actatgtaat aattatcctt cggctctttt aaaacatcct ttaatatttt
      661 tacatcctct aattcataag gtacaattat ttccgcatct atattcttaa gtttttcaat
      721 tagctcactt acagtaatta taggttcgac tgtgggaatg atatttcttt tgctctgttt
      781 agctgattct ttggctatct tttgccatct gtccagtttc ttttgttctc tgtccttttc
      841 attaagttta acaacggttc tttctgttat taaaggaata aagcttttca cacccaactc
      901 tacattttgc tgtataattg tttccatttt agttttttta ggtattccct gacaaagggt
      961 tatgtttatt tttgtttcat tattggaaaa tatctcttca accaaggtgc agactatatt
     1021 ttccgtattt atttctttta tttcagcaat gtattccttg ctttcagtca caactgaaat
     1081 tttatcactc ggctgtgccc gaagaatagt ttttaaatgc tttgcatcgc cgcctacaac
     1141 attaaccgta ttatttttta tattttcatc cgcacaaaaa tatctgaaca tttcttcctc
     1201 cttaatatta cacttccctg atgagatcct tcgcttacgc tcaggatgac agttttataa
     1261 ttatttctca ggatggcaat ttagcggtta cggataccca ttcttcgctt tcattgattt
     1321 ctattatttc aaatcccaag ttttcaaaat tatctgtaac ttcctgcaaa tattttttaa
     1381 ttattcctga agctataact aaaccgccct tttttaaaaa tttcgggaaa gtataactca
     1441 tgctcataat tacttgagca attatattaa ctgcaatcat gtcatactta ttgtatccga
     1501 ttttttcttg caaggccttg tcctcggtta catcacccaa gtacaccgtg aatctgtctt
     1561 ggttaatatt gttgagttca gcattttctc ttgctattcg aacagaattt tcgtcgatat
     1621 cgaccccgac acatttcttg gctccaagca gcaatgatgt tattgataat ataccgcttc
     1681 cgcatcccaa atccaacact tccatatctt tattcatgta tttaattatc tgctcaatac
     1741 aaaggcgtgt ggtatgatgc tgtcccgttc caaagctcat accggggtct att
//
LOCUS       DAOSHP010000163         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_67678_length_1779_cov_10.208817, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000163 DAOSHP010000000
VERSION     DAOSHP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67678_length_1779_cov_10.208817"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1641
                     /locus_tag="PLB33_09450"
     CDS             196..1641
                     /locus_tag="PLB33_09450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOG63793.1"
                     /translation="MEYNSTNDFYLIFKPVYNKDGKLTDYVLKYVSDSFCDAAGISPG
                     LILGKCFSEIAVENSDKLGFKDLYMVMNPYSKLKYATYLDNLERWYIISIFNADQNHK
                     GDLIIYYIDITHIKQGSRSRFVYSESFHNKLFHIKDRQKILYRDKLTGLYNKNFFEEE
                     LARLDTKRQLPISIIMGDINGLKLINDAFGHKMGDSILQKAAEIMTASFREEDIICRV
                     GGDEFVVLLPKTSEETALEIIERVKYKCEFNPLDYIRVNISLGAATKESEDTDIYEVL
                     KKAENKMYFKKLKESKEAKISMIKSLKSRLEKITYETKSHYERLKNLSMKMADILNLS
                     NREKEELMLLCEFHDIGKIGVSKSVLQKQGCLNDEEWEDIKRHSEIGYYIAKECKNAS
                     PVDELILLHHERWDGKGYPGLLKKDEIPVVARVFAIADAYDIMVTGTPYKDPMGNKQA
                     LNEIRDKAGSQFDPNLAEIFIKIMGEVEQVV"
BASE COUNT          695 a          252 c          354 g          478 t
ORIGIN      
        1 ataggtactt atactgacag gttgattgta aaaggcgttt tgctgaataa tgattaatca
       61 gaatcaaatt atcggtaatc aaaatgtaat tgaaatgtaa ccgaaaacaa attgctaaat
      121 aatagaattg taataggctt aagatataat attgataaac atatttatat aagacaatgt
      181 tttcgaggtg gcgagatgga atacaattca acaaatgatt tttatttaat atttaagcct
      241 gtatacaata aagatggaaa attaacagac tatgttttga aatatgtaag cgattctttt
      301 tgcgatgcag ccggcataag ccccggttta atattgggga aatgcttttc cgaaatagca
      361 gtagaaaatt ccgataaatt gggatttaaa gacttatata tggttatgaa tccatattct
      421 aaattgaagt atgcaacata tttagataac ttggagcgct ggtatattat cagtatattt
      481 aacgccgacc aaaatcataa gggtgatttg attatttact atattgatat tacacatatc
      541 aagcaaggaa gcaggagcag gtttgtttat tcagagagtt ttcataacaa actatttcat
      601 ataaaagaca gacaaaaaat attatacagg gacaaactga cgggtttata caacaagaat
      661 ttctttgaag aagagcttgc aagattagac acaaaaagac aactgcctat cagtataata
      721 atgggggata ttaacgggct gaaattaatc aatgatgcat tcggtcataa aatgggagac
      781 agcatattac aaaaggcagc tgaaattatg actgcttcat tcagggaaga agatataata
      841 tgcagagtgg gaggagatga atttgtagta ctgcttccca aaacatctga agaaactgca
      901 ttggaaatca ttgaaagagt aaaatataaa tgtgaattta atcctttgga ttacattagg
      961 gttaacataa gcttgggagc tgccacaaag gaatccgaag atacagatat atatgaagta
     1021 ttaaaaaaag cagaaaacaa aatgtatttc aaaaagctca aagaaagcaa agaagccaag
     1081 atatcgatga ttaagtccct aaaatcaagg cttgaaaaaa taacatacga aacaaagtct
     1141 cactatgaga gacttaagaa tctaagcatg aaaatggcgg atatattgaa tttgtcaaac
     1201 cgagaaaaag aagagctcat gcttctctgt gaattccatg atattgggaa aataggagtt
     1261 tcaaaaagtg ttctccaaaa gcaaggctgt ctaaacgacg aagaatggga agatataaag
     1321 cgccacagcg aaataggata ctatattgcc aaggaatgca agaacgcttc tccagtagac
     1381 gaacttatac tgctccatca cgagcgatgg gacggcaagg gttatccggg tttgcttaaa
     1441 aaggatgaaa tacctgttgt ggcaagagtc tttgcaatag cggatgcata tgacatcatg
     1501 gtaaccggca caccctacaa ggatcctatg ggaaataaac aagctctcaa tgaaataaga
     1561 gataaagcag gaagccagtt cgacccgaat cttgcggaga tatttattaa aataatggga
     1621 gaagtggagc aagtggtata acaagattga catcctgaac cacatagatg ttattctgaa
     1681 cgaagagaaa gccctcaatg attaatggta aaaaaatgtt taacaaccat ttattgaggt
     1741 atatataatt tacaatcact caaaattaaa actgattta
//
LOCUS       DAOSHP010000164         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_71797_length_1710_cov_15.446526, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000164 DAOSHP010000000
VERSION     DAOSHP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71797_length_1710_cov_15.446526"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLB33_09455"
     CDS             <1..282
                     /locus_tag="PLB33_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63794.1"
                     /translation="HKKAQALGFLTALTGLSTGNISAAGAGLLGASKKSMYTNFKKVK
                     SVKFSPGMNTVYLNESLNKNQIYAEKEDYPFIKEFILENCPKNVKIIEK"
     gene            318..>1710
                     /locus_tag="PLB33_09460"
     CDS             318..>1710
                     /locus_tag="PLB33_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOG63795.1"
                     /translation="MNKVKYCRKCGNAISDGVKFCRHCGALQYNQSKSPSISDSQPSK
                     KNTKGPDVIDNSIKQKQKEKYFNNNETKTVKKKRGIFGKIAALLLIIFIAVNIINFLS
                     NKPNEPGSILTTTGNDENKSISEYVLSDVKISDDDLNIPAQSGFVSPDEPEITIENTF
                     INFSSYNLEGTKEVKVKRLNQISDSEKGYTIQAYDFEIEGISEFNVPVYIEMPYYPVI
                     EDNLYEESTIFVQYYNENESKWEMIPSWINAEANTVAILTNHFSPFGVFQDDNLEDAK
                     KIAHLFQYYGKYKGPTTPVYTTGTNIQRYYQFVDEKTFQTYIDYKTVPVNDFATSFLN
                     VMNHGASAGEYSLYALSPEIYKDTISGFSNAGKLFVFSKIAYQWYNGVATSDIIISNI
                     FDLTEIAISGAGTLLAAPELTVIAGGVWLTGLTYDVGTTAYEYMSENEPRYLAYRLAS
                     ESGYIKYLPDEEIC"
BASE COUNT          649 a          265 c          311 g          485 t
ORIGIN      
        1 cataaaaaag cacaggcctt gggatttctt acggctttaa caggcctttc tacaggaaat
       61 atttctgcag caggagcagg gcttttggga gcatccaaaa aaagtatgta cacaaatttt
      121 aaaaaggtaa aatctgttaa attcagccct gggatgaata cagtttattt aaatgaatcc
      181 ttaaataaaa accagattta tgcagaaaaa gaagattatc catttataaa ggaatttatt
      241 ttggagaatt gtccgaaaaa tgtaaaaatt attgaaaaat agcaagcctc aaaatataaa
      301 tataaggggg aattgccttg aataaagtaa aatattgcag gaaatgcggc aatgcaatca
      361 gtgatggtgt taaattctgc agacattgcg gtgctttgca gtacaatcag tcaaaatcac
      421 catctattag cgattcgcaa ccttcgaaga aaaatacaaa aggaccggat gttattgata
      481 atagcattaa gcaaaagcaa aaagaaaaat atttcaacaa taatgaaaca aaaacagtga
      541 aaaagaaaag aggaatattt gggaaaatag ccgcactttt gctgataatt tttatagcag
      601 ttaatataat aaacttctta tcaaataagc ctaatgaacc aggcagtatt ttaaccacca
      661 ctggaaatga tgaaaacaaa agcataagtg aatatgtatt aagtgatgta aaaataagtg
      721 atgatgattt aaatatccct gcacagtcag gattcgtgag ccctgatgag ccggaaataa
      781 ctattgaaaa cacctttatc aattttagtt cttataattt ggaaggaaca aaggaagtaa
      841 aagtaaaaag attaaatcaa atcagtgaca gtgaaaaagg ctatacaatc caggcctatg
      901 attttgaaat agaaggtatt tctgaattta acgtgcctgt ctatattgaa atgccatact
      961 atccggtaat tgaagacaat ctttatgaag aaagcaccat atttgtccag tattacaatg
     1021 aaaacgaaag caaatgggag atgatacctt cctggataaa tgccgaagcc aacactgtag
     1081 caattctaac caatcatttt tcaccctttg gcgtatttca ggatgataat ttggaagatg
     1141 ctaaaaagat tgcccactta tttcaatatt atggaaagta taaaggacct acgacacctg
     1201 tatataccac cggaacaaat atccaaagat actatcaatt tgttgatgaa aaaacatttc
     1261 aaacctatat tgattataaa acagtaccgg taaatgactt tgcaacaagt tttttaaatg
     1321 ttatgaatca tggagcatca gcgggagagt attctttata tgctttatct cctgaaattt
     1381 acaaagatac tattagtggt ttcagcaatg cagggaaact tttcgttttt tcaaaaattg
     1441 cataccaatg gtacaatggg gtagctacat cagatattat aattagtaat atttttgacc
     1501 tcaccgaaat agccatatcc ggagccggaa cacttttagc agcacctgaa ctcacagtaa
     1561 ttgcaggagg agtctggctt acaggtttga cctatgatgt tggaactaca gcttatgaat
     1621 atatgtctga gaatgaacca agatatttag cctatcgttt agcaagtgaa tccggttaca
     1681 ttaaatattt gccggatgaa gaaatatgcg
//
LOCUS       DAOSHP010000165         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_72764_length_1695_cov_11.518902, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000165 DAOSHP010000000
VERSION     DAOSHP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72764_length_1695_cov_11.518902"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1695)
                     /locus_tag="PLB33_09465"
     CDS             complement(<1..>1695)
                     /locus_tag="PLB33_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63796.1"
                     /translation="IPYFWVKTDGRNLNLYLSHSNIGSSEIEGNIKISDLDQKIQLDN
                     ANVAANIDLGMLNKDLQGYVKVIAKGQGTMDKPELELNIVSEQAKYLKYTVSNLNLSG
                     TYNNDELSFNLPGLVFENQIINCSGNINPFYRTINAHLETLPISVKSQPYLATANLDV
                     YAELWDKYPYVDATIHQLDFNYNLVTLQGISGKIKFLPLFSPDNLYLDANISGDNGYN
                     IKVIGDILSRNLLLDAKFDNMEVAALYSQPEVIELNPYLNGDIKAIITGENIVLQTLL
                     GVKLDKPVPVNTPLDALGTFNYKTREGTLHLISENGMVNYQPLNLELSAMLKDNLLTV
                     QGFKFNDLISLSGAINLSDYQDLNFALNLNNIGYLDIVHYFPQLDINLPEFYGLNVFA
                     EYNRQNSGVLNANVYLSYIDLLAVTPFSLKLGLNGPVDSIQVSGNIHNTEQNILNLSG
                     FIGFKPEPNISLDASFKDLTIQDVLINSPVTGKLNGKAGITWHKIEKQKPEIELRADL
                     LSENLTIEDYTINTAIIKGQQTPRKLIVDSLLVQSTGLFKLNGSGAINYNALYNEY"
BASE COUNT          534 a          292 c          293 g          576 t
ORIGIN      
        1 aatattcgtt gtaaagtgca ttataattaa tagcgcctga gccgttaagc ttaaaaagac
       61 ctgtggattg aactaacaaa ctgtctacaa taagtttgcg gggtgtttgt tgacctttaa
      121 taatagctgt attaatagtg taatcttcaa tagtcaggtt ttccgaaaga aggtctgctc
      181 tcagttcaat ttccggtttt tgtttttcaa ttttatgcca ggtgattcct gcttttccat
      241 ttaattttcc tgttaccgga gaattgatga gaacatcttg aatggtgagg tctttaaaag
      301 aggcatccaa actaatattc ggctccggtt taaaaccaat aaaaccgctt aaattcagaa
      361 tgttttgttc cgtattgtga atattgcctg aaacctgaat gctgtctaca ggtccgttta
      421 atcctaactt cagagaaaaa ggagtaactg ctaaaagatc aatgtaagat aaataaacat
      481 tagcattcag aactcccgaa ttctggcgat tgtattcagc aaaaacattc agtccgtaaa
      541 attcaggtag attgatatcc agctgaggaa aatagtgcac aatatccaaa taaccgatgt
      601 tattgaggtt taaagcaaaa ttcagatcct gatagtcgga aagattaatt gctccgctta
      661 aactgattag gtcgttaaat ttaaaaccct ggacggtaag taagttatct tttagcatag
      721 cagataattc caaattcaga ggttgataat tcaccattcc gttttcactt ataagatgca
      781 aagttccttc cctagtttta taattaaagg ttcccaaggc atctaaagga gtatttacag
      841 gcacaggttt atccaacttt actcctaaaa gggtctgtaa tactatattt tcacctgtaa
      901 tgatggcttt tatgtctcca ttcagatagg gattcagctc aataacttca ggttgagaat
      961 agagtgctgc tacttccata ttatcaaact ttgcatctaa tagaagatta cgacttagaa
     1021 tatcacctat aaccttaata ttataaccgt tatctcctga tatattggca tctaaatata
     1081 gattatccgg gctgaaaaga ggtaaaaatt ttatcttacc gcttatacct tgtagcgtta
     1141 ccaggttata attaaaatcc agttggtgga tagtagcatc aacataagga tatttatccc
     1201 ataattctgc ataaacatct aaattggcag ttgccagata gggttgggac tttacgctta
     1261 tcggtaatgt ttccagatgc gcattgattg ttctgtaaaa aggatttata ttacctgaac
     1321 agttaattat ttgattttca aaaacgagcc caggtaaatt aaaagaaagc tcatcattat
     1381 tgtaggttcc ggataaatta agattgctga cagtatattt taggtatttt gcttgttcag
     1441 aaacaatatt aagttccaat tcgggtttgt ccattgtgcc ttgtcctttt gctattacct
     1501 ttacatagcc ctgaaggtct ttattaagca tacccaaatc aatatttgca gcgacattgg
     1561 cattatccaa ctgtatcttt tgatctaaat cgcttatttt tatatttcct tcaatttcac
     1621 tgctgccaat attgctgtga gaaaggtata aattaagatt tctgccatct gtttttaccc
     1681 aaaaataagg aatta
//
LOCUS       DAOSHP010000166         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_73302_length_1687_cov_4.525735, whole genome shotgun sequence.
ACCESSION   DAOSHP010000166 DAOSHP010000000
VERSION     DAOSHP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73302_length_1687_cov_4.525735"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1254
                     /locus_tag="PLB33_09470"
     CDS             76..1254
                     /locus_tag="PLB33_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOG63797.1"
                     /translation="MLYKNNYIEKLLGIKGVILRNIEENNTSITISIELEVKDHICPC
                     CGNKTRKIHDYRTQIIKEAPMFGKHTYYKYRKRRYVCSICNKRFYEDVSFAPRYHRMT
                     SRLVSLIIEKMRSTHSMKSIAEECNISVNTITRVFNYVNYSFRHLPGVLSIDEFKGNA
                     GKYKYQCILTDPKNKKVLDILEGREQHILTDYFKDFKDRKNVKYIIMDMWKPYKEVAE
                     AFFKNSTIVIDKYHFIRQANWAFERVRKSEQKKFADIRRKYFKRSRFLLLKRMKTLNE
                     EQLQAVEVMLKTSPKLSDAYILKEKFYEFVDSPDLESAKRNLKAWYLFAAACNVPEFN
                     ECVKTINNWEKYILNSFTCPYTNGYTEGTNNKIKVLKRNAYGVRNFKRFRNRILHVMS
                     "
     gene            complement(1456..>1687)
                     /locus_tag="PLB33_09475"
     CDS             complement(1456..>1687)
                     /locus_tag="PLB33_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63798.1"
                     /translation="DAEVDAVSVKNNMIKLGDKWFVVADNALMYTLDGNEYTDFDEFV
                     DLYDVAEGDTVDYVLNTDEEVAFMILYVEDNK"
BASE COUNT          644 a          269 c          290 g          484 t
ORIGIN      
        1 tataaaaaaa tgtagagcaa aatagaaaat tccattagaa taaagtcacc acaaccattc
       61 aaaggaggtt tctaaatgct ctacaaaaat aattatatcg aaaaactatt aggaattaaa
      121 ggggtaattt taagaaatat tgaagaaaat aatacaagca ttaccataag tattgaatta
      181 gaggtaaaag accatatatg tccttgctgt ggcaataaaa ccagaaagat acatgactac
      241 agaactcaaa taatcaaaga agctccaatg tttgggaagc atacatatta caaatacaga
      301 aaaagaagat atgtttgtag tatttgcaat aaaaggtttt atgaggatgt gtcttttgcc
      361 ccgagatatc acaggatgac atcaaggctt gtcagcctga ttattgaaaa gatgagaagc
      421 acacattcaa tgaaaagtat tgctgaagaa tgcaatattt ctgtaaatac cattaccagg
      481 gtttttaatt atgttaatta ctcttttagg cacctgccgg gtgtattatc catagatgaa
      541 tttaaaggta atgcaggcaa gtataaatat caatgtatat taaccgatcc taaaaacaaa
      601 aaagtacttg atatcttaga aggaagagaa caacatatat tgactgacta ttttaaagac
      661 tttaaagata gaaaaaatgt taaatatatt attatggaca tgtggaagcc atataaggaa
      721 gtagctgaag cgtttttcaa aaattccacc atagtaatcg ataaatatca ttttatacgt
      781 caggcaaact gggcatttga aagggtaaga aagagcgagc agaagaagtt tgccgatatc
      841 agaaggaaat atttcaaaag aagcaggttt ttgctgttaa aaaggatgaa aacccttaac
      901 gaagagcagc tccaggcagt cgaagtaatg ctaaaaacct cgccaaaatt aagtgatgca
      961 tacatattga aagaaaaatt ttatgaattt gtggacagcc ctgatcttga aagtgcgaaa
     1021 agaaatttaa aggcttggta cctgtttgca gctgcctgta atgtacccga atttaatgaa
     1081 tgtgtaaaga ctattaacaa ttgggaaaaa tatattttaa actcttttac ctgtccatat
     1141 acaaatggat atacggaagg gacaaacaac aaaattaagg ttcttaaaag gaacgcatac
     1201 ggtgtcagga atttcaaaag attcagaaac aggatacttc atgtgatgag ttagtcgacc
     1261 ggtgcaaact ctgaaacata gcaagtacac ccgcatgcgg gtgtacttgc tatgtttcaa
     1321 aacctataat taacataaat atgggtggtt aagctaaaaa ctgctcaaac acccatatta
     1381 ttaatcatat ttggtacctt tattctatgt caggattttc taaaccccaa caattgacaa
     1441 agaaccttaa ccatactatt tgttgtcttc tacatataga atcatgaaag caacttcttc
     1501 gtcagtattt aatacgtaat caactgtgtc accttctgct acatcataca agtcaacgaa
     1561 ttcatcgaag tctgtgtatt cattaccatc taatgtatac atcaatgcat tatcagcaac
     1621 tacaaaccat ttgtcaccta atttaatcat gttgtttttc acgcttacag cgtcaacttc
     1681 agcatct
//
LOCUS       DAOSHP010000167         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_74610_length_1668_cov_7.230006, whole genome shotgun sequence.
ACCESSION   DAOSHP010000167 DAOSHP010000000
VERSION     DAOSHP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74610_length_1668_cov_7.230006"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLB33_09480"
     CDS             complement(<1..373)
                     /locus_tag="PLB33_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63799.1"
                     /translation="MSISVKLKSNNQDGVSADCTRYYVHYLANLCNGGNTIEKHKSLL
                     KKSLPILISSLIILLIVFSPGTFSWIVDSFKPVIYGLVIAYLLDPVVMFLMRKLKVRR
                     KQGIFLACLILIGTISALIYKL"
     gene            complement(366..1668)
                     /gene="pyk"
                     /locus_tag="PLB33_09485"
                     /pseudo
     CDS             complement(366..1668)
                     /gene="pyk"
                     /locus_tag="PLB33_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
BASE COUNT          448 a          328 c          274 g          618 t
ORIGIN      
        1 atagtttata aattaaggcg cttatggttc ctattaaaat taagcacgcg agaaaaatac
       61 cctgttttct tcttaccttc agttttctca tcaaaaacat gacaacaggg tccagcaggt
      121 atgcaattac aagaccgtaa attaccggtt taaaggaatc aacaatccaa ctgaatgtgc
      181 cgggactgaa cactattagt aaaataataa gtgatgaaat caatattgga agagactttt
      241 tcaaaaggct tttatgtttt tctatcgtgt tacctccgtt acataagttt gctaagtaat
      301 gtacataata ccgagtacag tcagctgaaa ctccatcttg attattggat ttcagcttta
      361 cgctaattga caatatgcac ctttaataaa tttgtaatgc cggaaactcc tacgggtatt
      421 cctgcagtta ttattgctat atctcccttt tttaaatatc ctaattttag tgcagtttct
      481 actgattttt caattataag gtccgtagac tgcatttttc ctatctttat cgtatatact
      541 ccccaataaa gagccagttt cctcataacc ctatcgcttt gtgtagctgc aattatgggt
      601 gcaacgggtc tgtatcttga aacatttgca gctgtatagc ctgacgatgt agcggaaatt
      661 attgctgatg catttaaatc taaacaagac ttgcaggctg catagctgat agaatcggtt
      721 atgctgtaag ttgagccgag cctttttctt tccaaaagct cgccatagtt taatgagctt
      781 tcaatcataa gagatattct tgccattgtt tttaccgctt caagagggta gcagcctgaa
      841 gcagtttcac ctgaaagcat tatggcatct gtaccttcaa atattgctgt tgccacatcg
      901 gatacctcag ctctcgtagg tctgggattt ctaatcattg aatcgagcat ttgggttgcg
      961 gtaataaccg gctttcctgc ttgattgcat tttttgataa tcattttttg tacaagcggt
     1021 acttcttctg ccggtatttc cactcctaaa tctccccggg caaccattat tccatccgat
     1081 gcctcaataa tttcatctat gttttcaacg ccctgcctgt tttctatttt tgaaataatc
     1141 attatgtgag ctgcattttc tttttctaaa atttttctta tttgaataac atcatcagcc
     1201 tttctgataa atgatgctgc tatataatcg atttcttgct ctattccgaa tttaatatcg
     1261 cttacatcct tatcggtaag tgcaggtaaa ttaatttgaa catcgggtac attgatgcct
     1321 tttttgtttt taataatacc tccgtttttt acaaggcatt ttatatcagt attattgata
     1381 atttcaacag cttccatact tataaggccg tcgtctatga gtatcgtatc accttttttt
     1441 atgtcatttg caaattggtc gtaggtgaca ctgacaatag agtcatttcc ttggagctcc
     1501 cttgttgtaa gaataaattc ctgacccttt tttaattcaa cttgaccgtc tataaaatct
     1561 ctgattcgta tttcaggacc tttggtatca agcattatcg ctatagaggt atttagttct
     1621 tctcgcacct tttttatagt atttattttt tctttatgtt cttcatgt
//
LOCUS       DAOSHP010000168         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_74925_length_1664_cov_6.235550, whole genome shotgun sequence.
ACCESSION   DAOSHP010000168 DAOSHP010000000
VERSION     DAOSHP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74925_length_1664_cov_6.235550"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1664
                     /locus_tag="PLB33_09490"
     CDS             <1..>1664
                     /locus_tag="PLB33_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOG63800.1"
                     /translation="ARLSIDTRFITDNDDERVDVRAVSVDTNENKINELFIKKGKRTI
                     SDREIIVIEQFALAREIDTGDEIVLNINGRNHRFIVSAIASSPEYVYLMENEQVLLPD
                     YENFGVVYIEEDYLRKISGRGSFNEVVIRVDNQDNIDKTKDYLEDGLKKYGVMRVIGR
                     DDQLSNSMMNEEITGLEKVSQSIPIIFLLFAGIMLAVMLSRIVKKDRTSIGVLKAVGF
                     FDREIIVHYLKYAASVGIIGGFLGSIIGTLISGAMTEMYLEFINIPMLTFQIYYEKIL
                     VSVVLSLIFCVASGFWGIRNIIKINPAESMMPESPKQGKKILLEDIKFFWSKLSFSWR
                     MVYRNIFREKKKFLFIAAAVAITCSLMIMTMWMLEIIDVMLVKHYTEFAIMEYNVGFN
                     GFQDKRVLNEIKENISFNNMEGRIELPFEIKNGRHSKIVNVIGLEENTVFYTFRDLDG
                     NKVAIPKDGIVLSSNLANSLNADIGDRILMESFIPGRDGEYVTVKGIIEQSLGINGYI
                     NINYVNQKFLHKGIINGVYINSDDDVTAKLDDIINIMSIQSQEEMQ"
BASE COUNT          581 a          220 c          353 g          510 t
ORIGIN      
        1 atgcaaggct gtccatagat acaagattta tcaccgacaa cgacgatgaa agagtggatg
       61 taagggctgt ttctgttgac acaaatgaaa ataaaattaa tgaattattt ataaaaaaag
      121 gaaaacggac tatttctgat agagaaatta ttgttattga gcagtttgcc ctggcaaggg
      181 aaattgatac cggggatgaa atagttctta atataaacgg caggaaccac aggtttatag
      241 tatctgcgat tgcttcaagc ccggaatatg tgtacttgat ggaaaatgaa caggtgcttc
      301 taccggatta tgaaaacttc ggagttgttt atattgaaga agattatctg cgaaagatat
      361 ccggcagagg atcttttaat gaagtggtaa ttagagtaga caatcaggat aatattgata
      421 aaacaaagga ttatttagag gacgggctta aaaaatatgg tgtgatgagg gttataggca
      481 gagatgatca gctgagcaac agcatgatga atgaagagat taccggcctt gaaaaggtat
      541 ctcagtccat tcccatcata tttttgcttt ttgccggcat aatgcttgct gtaatgcttt
      601 cccgcattgt caaaaaggac agaacatcaa tcggagtatt aaaggcagta ggtttttttg
      661 atagagaaat aatcgttcac tatttgaaat atgctgcttc tgtaggaatt atcggaggtt
      721 ttttaggttc aataataggg actttgatct caggggcaat gactgaaatg tatttggaat
      781 ttattaatat tcccatgctg acctttcaaa tttattatga aaagattctt gtttcagtag
      841 tattatcatt aatattttgt gttgcttcag gtttttgggg tatcagaaac ataattaaaa
      901 ttaatcctgc cgagtctatg atgccggaat ctccaaagca gggaaaaaaa atacttttag
      961 aagatattaa atttttttgg agcaaattat cattttcatg gagaatggta tatcgaaata
     1021 tattcagaga aaagaaaaaa ttcctattca ttgcggctgc tgtcgctatt acctgcagcc
     1081 ttatgattat gaccatgtgg atgcttgaaa taattgacgt tatgctggta aagcattata
     1141 cggaatttgc cataatggaa tacaatgtgg gatttaacgg atttcaggat aaaagagttc
     1201 ttaatgaaat taaagaaaat ataagtttca ataatatgga gggaagaatt gaactgccct
     1261 ttgaaattaa aaacggcagg cattctaaaa tagtaaatgt aataggtctt gaagaaaata
     1321 ctgtatttta tacctttcgt gacttggatg gaaataaagt tgccattccc aaagatggaa
     1381 tagtactttc atcaaatttg gcaaactcac ttaatgcgga tataggagac aggatattga
     1441 tggaaagttt catacctggc agggacgggg aatatgtaac agtaaagggt ataatagagc
     1501 agagcttagg aattaacggt tacataaata ttaattatgt taaccaaaaa tttcttcata
     1561 aaggaattat caatggcgta tatatcaatt ccgatgacga tgtgaccgct aaattggatg
     1621 acataattaa tattatgtca attcaatcac aagaggaaat gcag
//
LOCUS       DAOSHP010000169         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_79433_length_1602_cov_8.457660, whole genome shotgun sequence.
ACCESSION   DAOSHP010000169 DAOSHP010000000
VERSION     DAOSHP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79433_length_1602_cov_8.457660"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1170)
                     /locus_tag="PLB33_09495"
     CDS             complement(610..1170)
                     /locus_tag="PLB33_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOG63801.1"
                     /translation="MDTNFKQTDYNIGEWALINKETSEIRELKKGKITAITPTGQVRV
                     NSKSYVFLDTERLKILRENGMTDGELGMLVLMSVNLSFVNNICMQDDNSPHTSSSISQ
                     KMGQTQQAVQRKLKRLIALGVLVKGKLPMQKHLGIVYMVNPYFIRRGKNFNAHLSTVF
                     NDIVKKQPPTIAEAHQKELDGNKFSQ"
BASE COUNT          470 a          316 c          278 g          538 t
ORIGIN      
        1 cataagcatc tgtaattcac agggttttaa gtgagcctct catgggactc gaacctaatt
       61 tatatgcagc ttaaaatcaa ttatatacat ctctcaaagt tcaataggtc ccgaatttga
      121 cacctattgc tcaaatttgc tcaatgaact ctctattgta aaaataggaa aaatatggaa
      181 tccgtatcat atggaaagta gaattttatc tcaaaatctg tgcttcattt ttcgtggatg
      241 agcaccattc attttgctgc taacgatctt ttatcagaca ggagaaaagc gatgtatccc
      301 catcaccccc attccccgct ttttcttttt ttcttacacc ctaccattcc attcaattat
      361 aaaatagcat taaaggccat taatgtctta atatataatt tggtttctac tattgatttt
      421 gtattccggc atgaatgatt attacctgac cagattttat ttcctctgaa tgaagcaggc
      481 tgacttggag gaagaatgaa cccctttttg acatgaacat ggacataact ctcccgttag
      541 gaaccgaatg ccatattaag gggcgtacga ctcttccatt tggtaaatac cagccaaggg
      601 tgtttaatgt cactgactga atttattgcc gtctaattcc ttctggtggg cttcagcgat
      661 agtcggtggt tgtttcttca caatatcatt gaataccgtg gatagatgtg cgttgaaatt
      721 cttgcctctg cgtatgaagt agggattcac catgtacacg atgcccaggt gtttttgcat
      781 gggtaatttg ccttttacca acacaccaag ggcaatcagc cttttgagct tcctttgtac
      841 tgcctgttgt gtttgtccca ttttctggct tatggaggat gaggtgtgtg gtgaattgtc
      901 gtcttgcatg cagatgttat tgacaaacga caggttgacc gacatcaaga ccaacattcc
      961 tagctcaccg tccgtcattc cgttctcccg taagatcttc agtcgttcag tatcaagaaa
     1021 aacatacgac ttggagttca cccttacctg cccggtggga gtgatggctg ttatctttcc
     1081 tttcttaagc tccctgattt cacttgtttc tttatttatc agagcccatt cgccgatatt
     1141 gtagtctgtt tgtttgaaat ttgtgtccat aaactttcta aaatttttac aaaagtaaca
     1201 aaatgaactt atatgttcat atctcatcat attttgaact ttaaagttca tataaaatct
     1261 gttataatgt tcttaattag ctctttataa tttcgccctt atatattatt attaaatact
     1321 attagtactt tcattttata actattcggc aatgaggaat aacctttaaa taaaagatat
     1381 aaatatccga atagacttaa taaaagcctg atttaataag caagagtggg tagagtgaac
     1441 cacggggaaa aagaaacggg aatattgaac acgccgaata aactctctct agataggaga
     1501 atatcacgag cgaattaata ttaccgaaca gaaaacccat ggccgtcata ttatatacag
     1561 tattttacaa gaaaaaatcg tcttctcctt gataatcaac tt
//
LOCUS       DAOSHP010000170         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_79865_length_1596_cov_9.555483, whole genome shotgun sequence.
ACCESSION   DAOSHP010000170 DAOSHP010000000
VERSION     DAOSHP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79865_length_1596_cov_9.555483"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLB33_09500"
     CDS             complement(<1..190)
                     /locus_tag="PLB33_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOG63802.1"
                     /translation="MKKAVTKNNGNLIETKVNTSRGRAKTHKIEWTEKTWNASAGCTK
                     ISSGCKNCYAETMAKRLQG"
     gene            complement(206..532)
                     /locus_tag="PLB33_09505"
     CDS             complement(206..532)
                     /locus_tag="PLB33_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOG63803.1"
                     /translation="MRKAVLQSEVDAILTDEKIMARLQHEKNHLKRLIQKYRLLGDRI
                     WAIDREYCAHTGSGVHEAYYSGGILTDKYLKGLRAFRIKVGALKPYGGFSKAYVVDIP
                     IERLNQ"
BASE COUNT          443 a          315 c          249 g          589 t
ORIGIN      
        1 ttccttgtaa acgtttagcc atagtttcgg catagcaatt tttacagcca gaacttattt
       61 tcgtgcaacc tgccgaagca ttccacgttt tctccgtcca ttcaatttta tgcgtctttg
      121 ctcttcctct tgatgtgtta acttttgttt caatcaagtt tccattgttt tttgttacag
      181 cttttttcat gatattctct tttaattatt gatttaatct ttcaatcggg atgtccacca
      241 catatgcttt tgagaagcca ccataaggtt taagtgcacc caccttaata cgaaatgctc
      301 tcaacccttt caggtattta tcggtcaata ttcctcctga ataatatgct tcatgaacac
      361 cgctacctgt atgagcacaa tactcacggt caattgccca aattctatcc ccaagcaatc
      421 tgtatttctg aattaacctt ttaaggtgat ttttttcgtg ctgcaatcgt gccattatct
      481 tttcatcagt caggattgca tcaacttctg attgtaaaac tgcttttctc ataatataac
      541 tatttaataa ttaataaatt ccacataaat aaccgccaaa atattgaact ccaattcatt
      601 gtccctaaaa catgactatc atgattatac cagagcataa tccgtagccg tgcttatgaa
      661 agcaaagcat tcaaaatgtt tcatttgtgg ttattgtggc agcctgaatt ggctacaatc
      721 tacagagcgt ttcttttgtg gttatggtag gtcgcctgta gagtgatgta aaagtaaata
      781 cttcgaattt ctctgtcaat agcttcaaat ttcactttga cgaatgaccc cctcttaatt
      841 cccaataaaa tgtaaagtgc tgattatcaa tttaactatt cttaattaag aatgcttatt
      901 gacgaaggct ttaaattttt acattaaggt ttatgggaaa tgccaatatt tgactgtttc
      961 atttgtaact attctcccat aaaacgtcag attttggttt gagggatgag tattatatct
     1021 cacaaaagag ttcactcata tctaattcca acgtctcgca gagttcaatt aatgtcagta
     1081 cggtcatgtt attcgcccct tctgctctgg aaagagtatt gcgatgcaaa ttcagatttt
     1141 tggcgagttc catcttggag tagccgttat ttagacgata ctcatgaata agtgaagcta
     1201 tgttttcaag tttcttataa tattcctgcg gatacgtttt ctttatcata tgttaatgtt
     1261 tcatataaat acatctctgc ccaatagatt gtgaggcaac acctacgata aatgttcttt
     1321 tctgttccat tcagcaccaa tattcccaat ttattgtccc attttcaatg cagtatctgc
     1381 tattaaatgg ctgttttaac gctgaatggg aagattttta gtgttatttc ggattttttg
     1441 tgcccacacg ttgattatca ccatcaattt tcttcaggaa atcataataa ttcactttat
     1501 ggaaatccag tatcttgagc aatgacgaaa ttttgaaata ggttttgcca ttttccaact
     1561 ggctgtatgc atttcgatgc atgtgtgtcc cttcaa
//
LOCUS       DAOSHP010000171         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1724
            NODE_84294_length_1542_cov_13.730330, whole genome shotgun
            sequence.
ACCESSION   DAOSHP010000171 DAOSHP010000000
VERSION     DAOSHP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863253
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:37:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,902
            CDSs (total)                      :: 1,885
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84294_length_1542_cov_13.730330"
                     /isolate="AMR_MDS_1724"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            467..>1542
                     /locus_tag="PLB33_09510"
     CDS             467..>1542
                     /locus_tag="PLB33_09510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOG63804.1"
                     /translation="MIKLEYPSKFDFYLKFKPIYNKEGKFIDYILTYASDSFADAVNI
                     SPKMILGKKFSEILVDMDVLGFKEFYSNIIPKSKIKHELYIEKFRRWYIINSFTDMSN
                     YENELIIYYVDITDIKNEQSIFTIDNNIYYLKDREILLYKDKLTGLYNKTFFEEELSR
                     LDTKRQLPISLIMGDINGLKLINDAFGHSMGDSVLKKAAEIMTSSFRDEDIISRVGGD
                     EFVILLPMTMEKTALEIVERVKKKCEKNPLEYIKINISFGVATKTSMEEDINKIYKKA
                     EDRMYFNKLKESKEAKLSMIEFLKHRLGKITYETKSHYDRLKELTMMMAEALDLPELE
                     KEELRLLSEFHDIGKIGVPMNILQ"
BASE COUNT          588 a          207 c          302 g          445 t
ORIGIN      
        1 gaagaaaacg gcgatgaact ctggacagga ttaaaagcaa ataaggtatg gccgtaatat
       61 taggaatatt aaaacaacca taaaataatt aatacgtata acccaaatgc agccatttca
      121 aatggttgca ttttttcata aggtttttgt ttattgcatg taatgtaggg tatatatatt
      181 ttaatgtaga aacatctata tttaatttga aggcatttcg ctggcaacta agtcttgctt
      241 gtatgaaaga attttttgct tatacccttg ctattcagag acatttctct gaccaccagg
      301 actggttttt aagaaagaga attgtccata tacagttaca caccaatctg taattgaaat
      361 gtaatcaaaa agtaattgaa atgaaactga tgaaaaatag aaatgtaatt gatgcggtct
      421 ataataagaa ttagcagtaa aggaagaata tcaatacgga ggtgaggtga taaaattgga
      481 atacccttct aaattcgact tttatttaaa gtttaagcca atatacaata aagaaggcaa
      541 atttattgat tatatattga cttatgccag tgattctttt gccgatgcgg ttaacatcag
      601 ccctaagatg attttaggca aaaaattctc agaaatatta gtggatatgg atgtacttgg
      661 atttaaggaa ttttattcaa atattattcc gaaatctaaa atcaaacatg aattgtatat
      721 agaaaagttc cgaagatggt acattattaa ttcatttact gatatgagca attatgaaaa
      781 tgagttgata atatactatg tggatataac agacataaag aacgagcaat ccatatttac
      841 tatagataat aacatttatt atctaaagga cagagaaata cttttataca aggataagtt
      901 gacaggatta tacaataaaa ccttctttga ggaagagctg tcaaggcttg atacgaaaag
      961 acagctgccc ataagtctga taatggggga tattaacgga cttaaactaa ttaatgacgc
     1021 atttgggcat agcatgggtg acagtgtact gaaaaaagca gcagagataa tgactagttc
     1081 atttagagat gaagatatta tcagcagggt aggcggcgat gagtttgtga ttttacttcc
     1141 gatgacaatg gagaaaactg ctttggaaat tgtcgaaaga gtaaagaaaa aatgcgagaa
     1201 aaatcctttg gaatatatta aaattaatat tagcttcgga gtggcaacca agacatctat
     1261 ggaagaagat attaacaaga tatacaaaaa agcagaagac aggatgtatt ttaacaaact
     1321 gaaggaaagc aaggaagcaa agctttccat gattgaattc ttgaagcaca ggctggggaa
     1381 aatcacatat gagactaaat ctcattatga ccggcttaaa gaacttacta tgatgatggc
     1441 agaggcattg gatcttccgg aactcgaaaa ggaagaactg agacttctca gtgaattcca
     1501 cgatatcggc aaaatcgggg tgccaatgaa tatccttcaa aa
//
